von Willebrand factor
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009111 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8Note: Hover over icons to view figure legend
Homology
BLAST of von Willebrand factor vs. Ensembl Human
Match: VWF (von Willebrand factor [Source:HGNC Symbol;Acc:HGNC:12726]) HSP 1 Score: 499.59 bits (1285), Expect = 3.751e-144 Identity = 353/1264 (27.93%), Postives = 557/1264 (44.07%), Query Frame = 2
Query: 443 STYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKG-IDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG---------------------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPF-----MCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNC------GNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVI------RECEECNC 4081
S+ CS+ + TFDG+++S+ GY ++ G + QN +S+ + +G I N + + +P+ S+G+ + + + K+ G D N V K CGLCG FN DD+ + + F NSW S + ER P ++ + + + + CQ + ++ C V + ++ +C+ +CEC C +Y+ C +G+ + + + C P C G E+ +C S C + C L + C+ +C+ GC CP + D +CV +CPCV Y G L +C TCIC + +W C NEEC G C ++G H+ +FD R+F F G C+Y D+ SI I+ C D+ + C R++ V + + + + L + + ++ + LP + I T +RL +++ W+G R+ +KLS Y K G+CGN+NGN+ DD +T G+ E ++ F +WK + C+ + ++ CAL+ + ++ + C L S F+ CH A + + C +DVC+C R C C A +SYA CA + ++WR C CP + Y QCG+ C TCR L + +C E C+EGCFCP G DE +CV + C C + G + P +I D C C +G CT + G K CP + C KTC N DL C C + GC CP V H C+ CPC+ K Y+ +T+ G N C+C+D KW C + C C T G HY +FDG + F G C YV+ +D+CG+ GTF+++ N C S C K + + + G N P T F + + IL + + V WD+ +S+ +++ + YQ KVCGLCGNFD +ND+ + + ++E+D V+F +SWKV S C +K P C ++ + C IL SD F+ C+ +V+ E Y C+ D C C+ DC CFC + Y C ++ + WRT CP +C + + +N N C +C + + C C+EGC CP G L CV+ + C + + ++ +G KV C+ C+C
Sbjct: 30 SSTARCSLFGSDFVNTFDGSMYSF---AGYCSYLLAGGCQKRSFSIIG--------DFQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYASKGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWAL---SSGEQWCERASPPSSSCNISSGEMQKGLWEQCQLLKSTSVFARCHPLVDPEPFVALCEKTLCEC----------AGGLECACPALLEYARTCAQEGMVLYGWTDHSACS-PVCPAGMEYRQCVSPCARTCQSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVHSG--KRYPPGTSLSRDCNTCIC-RNSQWIC-SNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCQDHSF---------SIVIETVQCADDRDAVCTRSVTVRLPGLHNSL-VKLKHGAGVAMDGQDVQLPLL---KGDLRIQHTVTASVRLSYGEDLQMDWDGRGRLLVKLSPVYAGKTCGLCGNYNGNQGDDFLTPSGLAEPRVEDFGNAWKLHGDCQDLQK--QHSDPCALNPRMTRFSEEACAVL-TSPTFEACHRAVSPLPYLRNCRYDVCSCSDGRE-----CLCGALASYAAACAGRGVRVAWREPGRCELNCPKGQV-YLQCGTPCNLTCRSLSYPDEECNEACLEGCFCPPGLYMDERG-DCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTMSGVPGSLLPDAVLSSPLSHRSKRSLSCRPPMVKLVCPADNLRAEGLECTKTCQNYDLECMSMGC-VSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGETVKIG----CNTCVCQDRKWNC-TDHVCDATCSTIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILVGNKGCSHPSVKCKKRVTILVEGGEIELFDGE-----VNVKRPMKDETHFE-VVESGRYIILLLGKALSVVWDRHLSISVVLKQTYQEKVCGLCGNFDGIQNNDLTSSNLQVEEDPVDFGNSWKVSSQCADTRKVPLDSSPATCHNNIMKQTMVDSSCRILTSDVFQDCNKLVDPEPYLDVCIYDTCSCESIGDCACFCDTIAAYAHVCAQHGKVVTWRTATLCPQSCEERNLRENGYECEWRYNSCAPAC-QVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQTCVDPEDCPVCE-VAGRRFASGKKVTLNPSDPEHCQICHC 1227 HSP 2 Score: 130.568 bits (327), Expect = 2.314e-29 Identity = 97/386 (25.13%), Postives = 171/386 (44.30%), Query Frame = 2
Query: 2984 ICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKF-IYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMC---SKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
+C +G + + + C +G +FDG + F G CSY+++ G + +F +I + K + +YL F FV G+ + + P Y ++ + + G + D + + ++++ ++Y NK CGLCGNF++ ++D + G + D +FA+SW + S C P C S + W + C++LKS S F +CH +V+ E + C CEC G +C C A+ +Y C + + L W + C C ++ C + C +C +++ N C++ C++GC CP+G CVE C CV + +Y G + R+C C C + C+
Sbjct: 21 LCAEGT----RGRSSTARCSLFGSDFVNTFDGSMYSFAGYCSYLLAG---GCQKRSFSIIGD--------FQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYAS-KGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWALSSGEQWCERASPPSSSCNISSGEMQKGLWEQ--CQLLKSTSVFARCHPLVDPEPFVALCEKTLCECAGGLECAC--PALLEYARTCAQEGMVLYGWTDHSACSPVCPAGMEYRQCVSPCARTC----QSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVH--SGKRYPPGTSLSRDCNTCICRNSQWICS 380 HSP 3 Score: 101.679 bits (252), Expect = 1.520e-20 Identity = 118/461 (25.60%), Postives = 178/461 (38.61%), Query Frame = 2
Query: 1601 CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETF-------DEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ--KTCTNLDLVCPKGQC----------QLPGCFCPENTVLHN-GECIKPFECPC 2911
C S H +TFD ++F G C Y VL N L + + N C + GC ++I V +++ L S E+ VN +S+PY GN +V + +R + + +++LS K + K G+CG + N +D M G D V W ++ + I+E + C + S C L F +CH F+ C D C H E CE +SYA C + + WRT + C CP ++ YN C C C S C + EGCFCP + +EA +C+ + +F + ++ C CTC +G + CT+ CP + A CG+C+ + N D CP+ +C +P C L N GEC F C C
Sbjct: 1950 CTGSSTRHIVTFDGQNFKLTGSCSY---------VLFQNKE-QDLEVILHNGACSPGARQGCMKSIEV----KHSALSVELHSDMEVTVNGRLVSVPYV-GGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQ--CLVPDSSH------CQVLL-LPLFAECHKVLAPATFYAICQQDSC-----------HQEQVCEVIASYAHLCRTNGVCVDWRTPDFCAMSCP-PSLVYNHCEHGCPRHCD-GNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQ----PCQICTCLSGRKVNCTTQP------CPTAKAP-TCGLCEVARLRQNADQCCPEYECVCDPVSCDLPPVPHCERGLQPTLTNPGECRPNFTCAC 2362 HSP 4 Score: 90.8929 bits (224), Expect = 2.446e-17 Identity = 92/371 (24.80%), Postives = 141/371 (38.01%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXX-CTKSIKFIYLNRVFQF-------VRG---SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWA--KAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV--DPITNLKYNTGDKVIRECEECNCYEG 4090
C +C H +FDG+ F+ GSCSYV+ + NK ++I N AC + C KSI+ + + V G S P V N G + H FT + + + + P+ + +K GLCG D ND R G + D W V Q+P C + + + C++L F +CH V+ +Y C D+C ++ C + Y C + + WRT + C +C V+ +C + CP C NV+ C EGCFCP CV +AC +C+ D + + + C+ C C G
Sbjct: 1946 CPCVCTGSSTRHIVTFDGQNFKLTGSCSYVLFQ----NKEQDLEVILHNGACSPGARQGCMKSIEVKHSALSVELHSDMEVTVNGRLVSVPYVGGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQ-------LSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTV-------QRPGQTCQPILEEQCLVPDSSHCQVLLLPLFAECHKVLAPATFYAICQQDSCHQEQ------VCEVIASYAHLCRTNGVCVDWRTPDFCAMSCPPSLVYNHCEHGCPRHC---DGNVSS---CGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQPCQICTCLSG 2286 HSP 5 Score: 56.225 bits (134), Expect = 8.054e-7 Identity = 73/351 (20.80%), Postives = 126/351 (35.90%), Query Frame = 2
Query: 476 HHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVY-----------APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCP-CVRDNG 1492
HI TFDG F Y LF + L + N + + C S+++ ++ L E V +P+ + V+ G+ + E+ F+ +I+ + +P T K GLCG + ++D+ + + + KT V W + P EQ L + + + L L L C + + +CQ + C Q C++ Y++ C G+ VDWR+ C C ++ C C ++C G C GC+CP ++ CVP+E C C+ ++G
Sbjct: 1956 RHIVTFDGQNFKLTGSCSYVLFQN--KEQDLEVILHNGA---CSPGARQGCMKSIEVKHSALSVELHSDMEVT-VNGRLVSVPYVGGNMEVNVYGAIMH-------EVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQC------LVPDSSHCQVLLL-------PLFAECHKVLAPATFYAICQQDSCHQEQV--------------CEVIASYAHLCRTNGVCVDWRTP-DFCAM-SCPPSLVYNHCEHGCPRHCD--GNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDG 2262
BLAST of von Willebrand factor vs. Ensembl Human
Match: MUC5AC (mucin 5AC, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7515]) HSP 1 Score: 490.73 bits (1262), Expect = 5.554e-141 Identity = 367/1280 (28.67%), Postives = 565/1280 (44.14%), Query Frame = 2
Query: 416 VTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISM-KITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT----FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEI--KLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCIDI-GKNVTDNTGCKKVCIEGCF--CPDG---LHWQNNECVERKACECVDP---ITNLKYNTGDKVI--RECEECNCYEGAFRCT 4105
V R +N ++ CS H KTFDG+VF +P Y +F + GAA Y FN +++Q + ++ ++ + +++ LV + +PF+ G+++ S+ K++ L ++++ D ++ + K CGLCG FNG+ ++++ N K F N DP+D C + +C++++ L C + V + YL+ C+ ++C C E++ + +C C +YS CT G+ DWR C Q +C N ++ EC S C C CE C+ GC+CP D T CVP KC CV + + Y G +C C C G+W C E C G C + G H+ TFD + + G C Y VL + ++ + C C ++ V + D Q + + +S E+ +N + LP V I + ST + ++ G+++ +++ +L+ K R + G+CGNFN + DD T GV E F ++KT C + R ++ C+LS YA C +L +++ F +CH+A ++ C D C C + C C A SSY CA + + L + +C K CP ++M Y+ S C TCR L E ++GC CP+G D+ K CV+ S+C C G E D CTCT+G C C M +FDC CQK+C LD+ C QC +PGC CP+ V G CI +CPC N Y TI G C N C C W C + C C +G+ HY +FDG+ + F G C Y + ++ CG K T F++++EN+ CG++ ++C+K+IK + G ++ + + Y + + ++ T G+ + WDK S+ I + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP P C+ P R+ WA+ +C IL +F CH V YY+ CV DAC CD G DC CFCTAV Y C+E + + WRT CP C G+ W + CG C +C + G + D G +EGC+ CP +CV + P + Y G V + C+ C C E CT
Sbjct: 68 VVRASNPAHNGRV-CSTWGSFHYKTFDGDVFRFPGLCNY-VFSEHCGAA---YEDFNIQL----RRSQESAAPTLSRVLMKVDGVVIQLTKGSVLVNGHPVLLPFSQSGVLIQQSSSYTKVEARLGLVLMWNHDDSLLLELDTKYANKTCGLCGDFNGMPV-----VSELLSHNTKLTPMEFGNLQKMDDPTDQ----------CQDPVPEPPRNCSTGFGICEELLHGQLFSGCVALVDVGSYLEACRQDLCFC-----EDTDLLSC---VCHTLAEYSRQCTHAGGLPQDWR-GPDFCPQ-KCPNNMQYHECRSPCADTCSNQEHSRACEDHCVAGCFCPEGTVLDDIGQTGCVPVSKCACVYNG--AAYAPGATYSTDCTNCTCS-GGRWSCQE-VPCPGTCSVLGGAHFSTFDGKQYTVHGDCSY---------VLTKPCDSSAFTVLAELRRCGLTDSETCLKS--VTLSLDGAQTVVVIKASGEVFLNQIYTQLPIS---AANVTIFRPSTFFIIAQTSLGLQLNLQLVPTMQLFMQLAPKLRGQTCGLCGNFNSIQADDFRTLSGVVEATAAAFFNTFKTQAACPNIRN--SFEDPCSLSVENEKYAQHWCSQLTDADGPFGRCHAAVKPGTYYSNCMFDTCNCERSED-----CLCAALSSYVHACAAKGVQLGGWRDGVCTKPMTTCP-KSMTYHYHVSTCQPTCRSLSEGDITCSVGFIPVDGCICPKGTFLDDTGK-CVQASNCPCYHRGSMIPNGESVHDSGAICTCTHGKLSCIGGQAPAP-VCAAPMVFFDCRNATPGDTGAGCQKSCHTLDMTCYSPQC-VPGCVCPDGLVADGEGGCITAEDCPCVHNEASYRAGQTIRVG---C-NTCTCDSRMWRCTDDP-CLATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQ-----EVPYTIRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACACDSGGDCECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRG-----LEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECT 1269 HSP 2 Score: 106.686 bits (265), Expect = 4.097e-22 Identity = 101/413 (24.46%), Postives = 153/413 (37.05%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVH---------------------------------SSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C + QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N CG+ SC +SI Y R +N I+ N PG S + E G+QV + + + +P K+ N G CG ++ R G + + W V S+ P PP C P C+++ S F+ CH V+ +Y+ CV D C D C+++E Y A C ++I + WR T CP TC +V++ CG P C EGCFCP+G+ + CV C+ P G V +C+EC C + T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVYGHFRVLVDNYFCGAEDGLSCPRSIILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNGIVVSRIGVKMYATIPELGVQVMFS-GLIFSVEVPFSKFANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSP--------ICQLILSKVFEPCHTVIPPLLFYEGCVFDRCHMT---DLDVVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKVYQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGP-HGEPVKVGHTVGMDCQECTCEAATWTLT 5309 HSP 3 Score: 91.2781 bits (225), Expect = 2.275e-17 Identity = 103/425 (24.24%), Postives = 166/425 (39.06%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL----SITIQNTNCQDNSETGCPRTIGVIIGEDEDQV-----AINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN----PTCESDRGIVEYK---------------GACALSRSYSD---YANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC-----RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKC-EFHGR-FYLPYEIRQDRCNNCTCTNGNWICT 2719
C + +C+ C GDPHYITFD ++ F +C Y V I P+ + + N C CPR+I I+ +D+V ++ + ++EI N +S ++ G +++ ++ + E GV+++++G +E+ SK + G CG + D+ T +G + W + P C G+ + + + +C +L S+ F+ CH+ F++ C D C LD C + YA CA +I + WR T +CP CP + + Y CG S C L EGCFCPEG T F +A+ CV +C HG + + + D C CTC W T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQ-----------IVPVYGHFRVLVDNYFCGAEDGLSCPRSI--ILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNG---IVVSRIGVKMYATIPELGVQVMFSGLIFSVEVPFSK-FANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSPIC-QLILSKVFEPCHTVIPPLLFYEGCVFDRC-----HMTDLD-VVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKV-YQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGPHGEPVKVGHTVGMD-CQECTCEAATWTLT 5309 HSP 4 Score: 76.6406 bits (187), Expect = 6.490e-13 Identity = 98/423 (23.17%), Postives = 171/423 (40.43%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQN--------DCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQ-DMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICK------QPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCV---PQEKCPCVRDNGESTYQQGYRLKL--NCETCICGMHGKWQCIENEEC----EGRCFISGDPHYITFD 1639
C+V H TFDG +S+ YTL + G +++ D F T+N CS ++KI +G + L + + ++P+ + +G ++ + L +L+D+ +I++ + ++CGLCG F+ I +D+ ++ + +V F NSW PS + ++ PC A + ++K+ Q ++ C + V+ +Y + C ++ C C + +C FC Y+ AC G+ V WR+ + IC P+ + CG C + C + + + L GCY CP D CV P P +Y+ G + NC++C+C G + E C G+ F GD Y T D
Sbjct: 903 CAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGK-------DSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQEVPYT-----IRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSW-KLSPSCPDALAPKD--PCTA------NPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACAC-------DSGGDC-ECFCTAVAAYAQACHEVGLCVSWRTPS-ICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVCTYNGQRFHPGDVIYHTTD 1295
BLAST of von Willebrand factor vs. Ensembl Human
Match: MUC5AC (mucin 5AC, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7515]) HSP 1 Score: 490.73 bits (1262), Expect = 5.554e-141 Identity = 367/1280 (28.67%), Postives = 565/1280 (44.14%), Query Frame = 2
Query: 416 VTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISM-KITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT----FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEI--KLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCIDI-GKNVTDNTGCKKVCIEGCF--CPDG---LHWQNNECVERKACECVDP---ITNLKYNTGDKVI--RECEECNCYEGAFRCT 4105
V R +N ++ CS H KTFDG+VF +P Y +F + GAA Y FN +++Q + ++ ++ + +++ LV + +PF+ G+++ S+ K++ L ++++ D ++ + K CGLCG FNG+ ++++ N K F N DP+D C + +C++++ L C + V + YL+ C+ ++C C E++ + +C C +YS CT G+ DWR C Q +C N ++ EC S C C CE C+ GC+CP D T CVP KC CV + + Y G +C C C G+W C E C G C + G H+ TFD + + G C Y VL + ++ + C C ++ V + D Q + + +S E+ +N + LP V I + ST + ++ G+++ +++ +L+ K R + G+CGNFN + DD T GV E F ++KT C + R ++ C+LS YA C +L +++ F +CH+A ++ C D C C + C C A SSY CA + + L + +C K CP ++M Y+ S C TCR L E ++GC CP+G D+ K CV+ S+C C G E D CTCT+G C C M +FDC CQK+C LD+ C QC +PGC CP+ V G CI +CPC N Y TI G C N C C W C + C C +G+ HY +FDG+ + F G C Y + ++ CG K T F++++EN+ CG++ ++C+K+IK + G ++ + + Y + + ++ T G+ + WDK S+ I + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP P C+ P R+ WA+ +C IL +F CH V YY+ CV DAC CD G DC CFCTAV Y C+E + + WRT CP C G+ W + CG C +C + G + D G +EGC+ CP +CV + P + Y G V + C+ C C E CT
Sbjct: 68 VVRASNPAHNGRV-CSTWGSFHYKTFDGDVFRFPGLCNY-VFSEHCGAA---YEDFNIQL----RRSQESAAPTLSRVLMKVDGVVIQLTKGSVLVNGHPVLLPFSQSGVLIQQSSSYTKVEARLGLVLMWNHDDSLLLELDTKYANKTCGLCGDFNGMPV-----VSELLSHNTKLTPMEFGNLQKMDDPTDQ----------CQDPVPEPPRNCSTGFGICEELLHGQLFSGCVALVDVGSYLEACRQDLCFC-----EDTDLLSC---VCHTLAEYSRQCTHAGGLPQDWR-GPDFCPQ-KCPNNMQYHECRSPCADTCSNQEHSRACEDHCVAGCFCPEGTVLDDIGQTGCVPVSKCACVYNG--AAYAPGATYSTDCTNCTCS-GGRWSCQE-VPCPGTCSVLGGAHFSTFDGKQYTVHGDCSY---------VLTKPCDSSAFTVLAELRRCGLTDSETCLKS--VTLSLDGAQTVVVIKASGEVFLNQIYTQLPIS---AANVTIFRPSTFFIIAQTSLGLQLNLQLVPTMQLFMQLAPKLRGQTCGLCGNFNSIQADDFRTLSGVVEATAAAFFNTFKTQAACPNIRN--SFEDPCSLSVENEKYAQHWCSQLTDADGPFGRCHAAVKPGTYYSNCMFDTCNCERSED-----CLCAALSSYVHACAAKGVQLGGWRDGVCTKPMTTCP-KSMTYHYHVSTCQPTCRSLSEGDITCSVGFIPVDGCICPKGTFLDDTGK-CVQASNCPCYHRGSMIPNGESVHDSGAICTCTHGKLSCIGGQAPAP-VCAAPMVFFDCRNATPGDTGAGCQKSCHTLDMTCYSPQC-VPGCVCPDGLVADGEGGCITAEDCPCVHNEASYRAGQTIRVG---C-NTCTCDSRMWRCTDDP-CLATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQ-----EVPYTIRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACACDSGGDCECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRG-----LEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECT 1269 HSP 2 Score: 106.686 bits (265), Expect = 4.097e-22 Identity = 101/413 (24.46%), Postives = 153/413 (37.05%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVH---------------------------------SSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C + QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N CG+ SC +SI Y R +N I+ N PG S + E G+QV + + + +P K+ N G CG ++ R G + + W V S+ P PP C P C+++ S F+ CH V+ +Y+ CV D C D C+++E Y A C ++I + WR T CP TC +V++ CG P C EGCFCP+G+ + CV C+ P G V +C+EC C + T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVYGHFRVLVDNYFCGAEDGLSCPRSIILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNGIVVSRIGVKMYATIPELGVQVMFS-GLIFSVEVPFSKFANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSP--------ICQLILSKVFEPCHTVIPPLLFYEGCVFDRCHMT---DLDVVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKVYQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGP-HGEPVKVGHTVGMDCQECTCEAATWTLT 5309 HSP 3 Score: 91.2781 bits (225), Expect = 2.275e-17 Identity = 103/425 (24.24%), Postives = 166/425 (39.06%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL----SITIQNTNCQDNSETGCPRTIGVIIGEDEDQV-----AINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN----PTCESDRGIVEYK---------------GACALSRSYSD---YANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC-----RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKC-EFHGR-FYLPYEIRQDRCNNCTCTNGNWICT 2719
C + +C+ C GDPHYITFD ++ F +C Y V I P+ + + N C CPR+I I+ +D+V ++ + ++EI N +S ++ G +++ ++ + E GV+++++G +E+ SK + G CG + D+ T +G + W + P C G+ + + + +C +L S+ F+ CH+ F++ C D C LD C + YA CA +I + WR T +CP CP + + Y CG S C L EGCFCPEG T F +A+ CV +C HG + + + D C CTC W T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQ-----------IVPVYGHFRVLVDNYFCGAEDGLSCPRSI--ILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNG---IVVSRIGVKMYATIPELGVQVMFSGLIFSVEVPFSK-FANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSPIC-QLILSKVFEPCHTVIPPLLFYEGCVFDRC-----HMTDLD-VVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKV-YQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGPHGEPVKVGHTVGMD-CQECTCEAATWTLT 5309 HSP 4 Score: 76.6406 bits (187), Expect = 6.490e-13 Identity = 98/423 (23.17%), Postives = 171/423 (40.43%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQN--------DCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQ-DMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICK------QPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCV---PQEKCPCVRDNGESTYQQGYRLKL--NCETCICGMHGKWQCIENEEC----EGRCFISGDPHYITFD 1639
C+V H TFDG +S+ YTL + G +++ D F T+N CS ++KI +G + L + + ++P+ + +G ++ + L +L+D+ +I++ + ++CGLCG F+ I +D+ ++ + +V F NSW PS + ++ PC A + ++K+ Q ++ C + V+ +Y + C ++ C C + +C FC Y+ AC G+ V WR+ + IC P+ + CG C + C + + + L GCY CP D CV P P +Y+ G + NC++C+C G + E C G+ F GD Y T D
Sbjct: 903 CAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGK-------DSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQEVPYT-----IRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSW-KLSPSCPDALAPKD--PCTA------NPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACAC-------DSGGDC-ECFCTAVAAYAQACHEVGLCVSWRTPS-ICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVCTYNGQRFHPGDVIYHTTD 1295
BLAST of von Willebrand factor vs. Ensembl Human
Match: MUC5B (mucin 5B, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7516]) HSP 1 Score: 473.781 bits (1218), Expect = 1.838e-135 Identity = 353/1252 (28.19%), Postives = 544/1252 (43.45%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYE-SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKL--GTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCT-----GKRVW--KNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKV--IRECEECNCYEGAFRC 4102
CS H KTFDG+VF +P Y C A Y FN + + ++ + Q ++L N L+ + ++P++ G++V G +IK+ L L++ + + + + CGLCG FNG+ + FN+ N + + + D + + P A + + +C + C + V YL C ++C C T P +TF + Q ++A G +WR +C + N Q ECGS C C CE C+ GC+CP D + C+P +CPC +G TY G C +C C G WQC ++ C G C + G H T+D + + G C Y +D+ + + +T N NC V + D AI + + + +N + LP N + +++ + + ++ ++L ++ ++ G+CGNFN N+ DD GV E F +WK C + R ++ C+LS +YA C +L + + F +CHS N F C D C C + C C A SSY CA + + LS + +C K CP ++ +Y C TCR L E+ ++GC CP G TF A CV +C C HG P E+ D C+CT G C + C M + DC C ++C LD+ C C + GC CP V +G CI +CPC N Y +TI +C N C C++ +W C ++ C G C +G+ H+ +FDG + F+GSC Y++++D+CG+ GTF++++ENI CG++ ++C+K+IK + G+ V+R P G P + F++ G+ V WD+ S+ I + + Y+ +VCGLCGNFD ND RS + D + F +SWK+ SCP P C+ P R+ WA+ +C IL +F C V+ YY+ CV DAC CD G DC CFCTAV Y C++ + + WRT + CP C G W + CG C +C +N + + +EGC+ CP + N + CV + C C D N Y+ G +V C+ CNC +C
Sbjct: 77 CSTWGDFHYKTFDGDVFRFPGLCNYVFSEHCRAA----YEDFNVQ---LRRGLVGSRPVVTRVVIKAQGLVLEASNGSVLINGQREELPYSRTGLLVEQSGDYIKVSIRLVLTFLWNGEDSALLELDPKYANQTCGLCGDFNGLPA-----FNEFYAHNARLTPLQFGNLQKLDGPTEQCPDPLPLPAGNCTDEE------GICHRTLLGPAFAECHALVDSTAYLAACAQDLCRC-PTCP--------CATFVEYSRQCAHA---GGQPRNWR-CPELCPRTCPLNMQH-QECGSPCTDTCSNPQRAQLCEDHCVDGCFCPPGTVLDDITHSGCLPLGQCPCT--HGGRTYSPGTSFNTTCSSCTCS-GGLWQC-QDLPCPGTCSVQGGAHISTYDEKLYDLHGDCSYVLSKKCADSSFTVLAELRKCGLT-DNENCLK----------AVTLSLDGGDTAIRVQADGGVFLNSIYTQLPLS-AANITLFTPSSFFIVVQTGLGLQLLVQLVPLMQVFVRLDPAHQGQMCGLCGNFNQNQADDFTALSGVVEATGAAFANTWKAQAACANARN--SFEDPCSLSVENENYARHWCSRLTDPNSAFSRCHSIINPKPFHSNCMFDTCNCERSED-----CLCAALSSYVHACAAKGVQLSDWRDGVCTKYMQNCP-KSQRYAYVVDACQPTCRGLSEADVTCSVSFVPVDGCTCPAG-TFLNDAGACVPAQECPCYAHGTVLAPGEVVHDEGAVCSCTGGKLSCLGASLQKSTGCAAPMVYLDCSNSSAGTPGAECLRSCHTLDVGCFSTHC-VSGCVCPPGLVSDGSGGCIAEEDCPCVHNEATYKPGETI---RVDC-NTCTCRNRRWEC-SHRLCLGTCVAYGDGHFITFDGDRYSFEGSCEYILAQDYCGDNTTHGTFRIVTENIPCGTTGTTCSKAIKLFVESYELILQEGTFKAVARG---PGGDPPYKIRYMGI---FLVIETHGMAVSWDRKTSVFIRLHQDYKGRVCGLCGNFDDNAINDFATRSRSVVGDALEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACRSQVDSTKYYEACVNDACACDSGGDCECFCTAVAAYAQACHDAGLCVSWRTPDTCPLFCDFYNPHGGCEWHYQPCGAPCLKTC----RNPSGHCLVDLPGLEGCYPKCPPSQPFFNEDQMKCVAQ--CGCYDKDGNY-YDVGARVPTAENCQSCNCTPSGIQC 1252 HSP 2 Score: 91.6633 bits (226), Expect = 1.656e-17 Identity = 98/398 (24.62%), Postives = 162/398 (40.70%), Query Frame = 2
Query: 2978 PCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXX----CTKSIKFIYLNR----VFQFVRGSNP--IVSRNTNYPPGGPTAFYWTSDVDTHFILFTME--GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS----CPVPQ-KPPFMCSKRPDREPWAKAK------CEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCP-DGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGA 4093
P D C+ + +C IC WG +HY +FDG + F+G+C+YV+ + + G L +N C +S+++ C +++ Y + V G I+ G S + T + + G+ V ++ + + + N G CG +D R G + A +W V S C P PP P C+++ S F +CH++V ++ C++D C RG V C ++E Y C + WR T C TC +V+K CG I P +C N + + + EGCFCP D + + + + +AC CV P K+ G++ + C+ C C EG+
Sbjct: 5055 PIKVSDPSQPCDFHYECECICSMWGGSHYSTFDGTSYTFRGNCTYVLMREIHA-RFGNLSLYLDNHYCTASATAAAARCPRALSIHYKSMDIVLTVTMVHGKEEGLILFDQIPVSSGFSKNGVLVSVLGTTTMRVDIPALGVSVTFNGQVFQARLPYSLFHNNTEGQCGTCTNNQRDDCLQRDGTTAASCKDMAKTWLVPDSRKDGCWAPTGTPPTASPAAPVSSTPTPTPCPPQPLCDLMLSQVFAECHNLVPPGPFFNACISDHC---RGRLEVP-CQSLEAYAELCRARGVCSDWRGATGGLCDLTCPPTKVYKPCGPIQPATC-----NSRNQSPQLEGMAEGCFCPEDQILFNAHMGICVQACPCVGPDGFPKF-PGERWVSNCQSCVCDEGS 5441 HSP 3 Score: 86.2705 bits (212), Expect = 7.180e-16 Identity = 126/548 (22.99%), Postives = 190/548 (34.67%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETG---CPRTIGVIIGEDEDQVAINLLSSSE---IKVNFVKISLPYQHTGNTKVLIDQVSTDILRLK-SETGVEILWNGG---SRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSW----KTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYE---SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI--CTSNDCEGK---------------------EFCPKSMAWFDCGICQKTCTNLDLVCPKGQ----CQLPGCFCPE----------NTVLHNGECIKPFECPCYMNNKMYSE-NDTIVRGPANCQNPCICKDGKWVCEQNKQCSGIC 3040
C + ECE C + G HY TFD + F G+C Y + ++ LS+ + N C ++ CPR + + + + + ++ E I + + +S + G VL+ + T +R+ GV + +NG +R+ L + G CG N+ DD + G +W C + G A S L + S+ F +CH+ FF+ C D C G L+ C++ +YA C + WR T LC CP + Y CG +TC +S E EGCFCPE + F+ CV+ C G P E C +C C G+ C C+ + CP+++ C TC VCP GQ Q G CP N + P PC+M + + D V+ + N C G QC G C
Sbjct: 5065 CDFHYECECICSMWGGSHYSTFDGTSYTFRGNCTYVLMREI-------HARFGNLSLYLDNHYCTASATAAAARCPRALSIHYKSMDIVLTVTMVHGKEEGLILFDQIPVSSGFSKNG---VLVSVLGTTTMRVDIPALGVSVTFNGQVFQARLPYSL---FHNNTEGQCGTCTNNQRDDCLQRDGTTAASCKDMAKTWLVPDSRKDGCWAPTGTPPTASPAAPVSSTPTPTPCPPQPLCDLMLSQVFAECHNLVPPGPFFNACISDHC------RGRLE-VPCQSLEAYAELCRARGVCSDWRGATGGLCDLTCPPTKV-YKPCGPIQPATCNSRNQSPQLEGMAEGCFCPEDQILFNAHMGICVQACPCVGP-DGFPKFPGERWVSNCQSCVCDEGSVSVQCKPLPCDAQGQPPPCNRPGFVTVTRPRAENPCCPETV----CVCNTTTCPQSLPVCPPGQESICTQEEGDCCPTFRCRPQLCSYNGTFYGVGATFPGALPCHMCTCLSGDTQDPTVQCQEDACNNTTCPQGFEYKRVAGQCCGEC 5586
BLAST of von Willebrand factor vs. Ensembl Human
Match: MUC2 (mucin 2, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7512]) HSP 1 Score: 454.521 bits (1168), Expect = 4.033e-131 Identity = 366/1253 (29.21%), Postives = 564/1253 (45.01%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDF-NQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPK---KCPNENM-KYNQCGSRCTSTCRLLQE--SKCKE--ECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKG-QCQ-----------LPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC---------PHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CP-DGLHWQNN--ECVERKACECVDPITNLKYNTGDKVIRE--CEECNC 4081
CS H KTFDG+VF +P Y DC G+ F V+ Q S+ +T+ TI L A++ P S G+++ ++ K+ L ++++R+ + + CGLCG +NG+ S Y +F + + + F N P + E E P + + + C+ ++ CQ V ++ YL CQ + C C P T ST + Q S+A G +WR+AT +C + C + E GS C C L + CE + GC+CP +D + CVP +C C R +G Y G + +CE C+C G+W C ++ C G C + G H TFD + + F G C Y ++ +AP T + T C +T V++ D+ + + S + +N ++++LP+ T + V ++ + +++ ++ + L + + +V G+CGNFNG E DD T G+ E F +WK +C ++ C+L+ ++YA C L ++E F +CHSA + +++ +C +D C C + C C A SSYAR C + + L WR +C K CPN + YN + C TCR L E S C E ++GC CP+ DE + CV ++ C C G + ++ + C C +G C G+ C DC T L P+ CQ + GC CP+ + G C+ ECPC NN +YS I +C N C CK G+WVC Q C G C +G HY +FDGKY++F G CSYV +D+CG + LG+F +I+EN+ CG++ +C+K+IK I++ R + + +V + G Y T +V + ++ + GI V WDK ++ I + Y+ VCGLCGNFD R +ND R + ++F +SWK +CP P CS P R WA+ +C ILKS F CH V+ + +Y+ CV D+C CD G DC CFC+AV Y C + + WRT + C PH C ++ CGN ++C I + N +EGC+ CP D ++ + +CV C C + + Y G V E C+ C C
Sbjct: 37 CSTWGNFHYKTFDGDVFRFPGLCDYNFASDCRGSYKEFAVHLKRGPG---QAEAPAGVESILLTIKDDTIYL--TRHLAVLNGAVVSTPHYSPGLLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDYNGLQS--YSEFLSDGVLFSPLEFGNMQKINQPDVVCEDPEEEVAPASCSEHRAE---------CERLLTAEAFADCQDLVPLEPYLRACQQDRCRC----PGGDTC--VCSTVAEFSRQCSHA---GGRPGNWRTAT-LCPK-TCPGNLVYLESGSPCMDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHC-RLHGH-LYTPGQEITNDCEQCVCNA-GRWVC-KDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAKGDHNDSYALLGELAPCGSTDKQT---------CLKT--VVLLADKKKNVVVFKSDGSVLLNELQVNLPHV-TASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSSCHDKLDWLD--DPCSLNIESANYAEHWCSLLKKTETPFGRCHSAVDPAEYYKRCKYDTCNCQNNED-----CLCAALSSYARACTAKGVMLWGWR-EHVCNKDVGSCPNSQVFLYNL--TTCQQTCRSLSEADSHCLEGFAPVDGCGCPDHTFLDEKGR-CVPLAKCSCYHRGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQS-CTAPKIHMDC----SNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKI---KVDC-NTCTCKRGRWVCTQ-AVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYCGQNSSLGSFSIITENVPCGTTGVTCSKAIK-IFMGRTELKLEDKHRVVIQRDE----GHHVAYTTREVGQYLVVESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHMVVSSELDFGNSWKEAPTCPDVSTNPEPCSLNPHRRSWAEKQCSILKSSVFSICHSKVDPKPFYEACVHDSCSCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPPHECEWH--YEPCGNRSFETCRTI-NGIHSNISVSY--LEGCYPRCPKDRPIYEEDLKKCVTADKCGCY--VEDTHYPPGASVPTEETCKSCVC 1213 HSP 2 Score: 172.17 bits (435), Expect = 4.130e-42 Identity = 144/509 (28.29%), Postives = 235/509 (46.17%), Query Frame = 2
Query: 2675 RCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGI-CQKTCTNLDLVCPKGQCQLPGCFCPENTV---LHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRN---TNYPPGGPTAFYWTSDVDTHFILFTME---GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFM---CSKRPDREPWAKAKCEILKSDS--FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVC------------IEGCFCPDGLHW-QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
+C++ GNW T+ C + CP ++ + + G C TC++L++ + ++ GCFCPE TV + + C+ +C C ++ +Y+ I +C+ C+C G+WVC ++ C G C G +H +FDGK + F G C YV+++ G+ ++ L+ E CGS+ +C K++ + + V S+ V N N P TA + ++ I+ +M +QV+ M L + + + Q +V GLCGNF+ +D + SG +E FA++WK SSC K ++ CS + +A+ C +LK F +CH V+ YYKRC D C C +DC+C A+ Y C + L WR H C K+ G+ CP+S + + T C+ + ++GC CPD + CV C C L GD V+R+ E C C +G C ++
Sbjct: 274 QCSHAGGRPGNWR-TATLC--PKTCPGNLVYLESGSPCMDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITN---DCEQ-CVCNAGRWVC-KDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAK---GDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVLLNELQVNLP--HVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSSCH--DKLDWLDDPCSLNIESANYAEHWCSLLKKTETPFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCLC--AALSSYARACTAKGVMLWGWRE-----HVCN-----KDVGS-CPNSQVFLYNLTTCQQTCRSLSEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYH--RGLYLEAGDVVVRQEERCVCRDGRLHCRQI 752 HSP 3 Score: 138.272 bits (347), Expect = 9.219e-32 Identity = 102/384 (26.56%), Postives = 180/384 (46.88%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIK---------FIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDI----VNFADSWKVHSSCPVPQKP-----PFMCSKRPDREPWAKAKCE-ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK---WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQ---NNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
+C TWG HY++FDG F F G C Y +F + G++K + ++ G + ++ IYL R + G+ V +Y PG D + +++ G+ + W++ +L + + K++N CGLCG++ N +++ S + + + F + K++ V + P P CS+ +A+CE +L +++F C D+V +E Y + C D C C GD CV C+ V ++ +C+ G + WRT CP TC G V+ G+ C D+C ++ ++ C++ ++GCFCP+G + ++ CV C C + Y G ++ +CE+C C G + C L
Sbjct: 36 VCSTWGNFHYKTFDGDVFRFPGLCDY----NFASDCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGA---VVSTPHYSPG-----LLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDY-----NGLQSYSEFLSDGVLFSPLEFGNMQKINQPDVVCEDPEEEVAPASCSEH-------RAECERLLTAEAFADCQDLVPLEPYLRACQQDRCRCPGGDTCV--CSTVAEFSRQCS--HAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTC----SHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCR--LHGHLYTPGQEITNDCEQCVCNAGRWVCKDL 385
BLAST of von Willebrand factor vs. Ensembl Celegans
Match: T01D3.6 (pep chromosome:WBcel235:V:13716824:13720402:1 gene:WBGene00011330.1 transcript:T01D3.6b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T01D3.6) HSP 1 Score: 78.5666 bits (192), Expect = 4.291e-14 Identity = 87/378 (23.02%), Postives = 156/378 (41.27%), Query Frame = 2
Query: 1607 ISGDPHYITFDRRHFFFDGHCEYTAVM--TTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIK-VNFVKISLP-YQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS--KKYREKVT--GMCGNFNGNEMDDS-------MTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGA--CALSRSYSD-------YANKVCHKLYESE----EFKKCHSAAN--ADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALE----NINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDE---AAKECVKISDCKC 2629
+SGDPHY+T+D F + G C Y TT L S A + + + SE + D + I++ S+ VN V++ P Y NT + + S ++++ GV + + + + +++ ++ T G+ GN +G ++DD + K + + + KT T +D+ ++ G C ++ + A++ C+ + ++E F C N + F+ C +D+C RN A +C F+ + + C L +N +WR CP CP N + C S C STC C + C++GC C G D + +C+++ C C
Sbjct: 190 MSGDPHYVTYDGLRFDYQGTCPYVFSQPCTTLPAPYLWYSVRAKNELPGKGYHISQVSE----------VEVDLHNLTIHVDGRSKTALVNGVQVLTPWYFPNKNTWTVRVRFSGSTFTIENDQGVVVTFTTYNSLCVQVPDIPEFNGATTLCGLAGNIDGKKLDDVVNKNGSVLAIKSSRQPENNNHADFMKTEDTWITDKFLILRPGQENCINGQTLDNNTNCDVATASQTCYPIQQAEMGLGTFGACQGLGNDTLEDFYYNCIYDIC-----RNPAF---KCTEFTYFLQYCQLALPQVAMNQNWRAQINCPLACP-LNAHPSTCTSSCPSTCAEPFPEYCDQGCVDGCECDPGYVIDNTVTGSIKCIRLDQCGC 548 HSP 2 Score: 62.003 bits (149), Expect = 4.138e-9 Identity = 91/414 (21.98%), Postives = 149/414 (35.99%), Query Frame = 2
Query: 2963 ANCQNPCICKDGKWVCEQNKQCSGICKTW---GETHYESFDGKYFEFQGSCSYVISED--FCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRN---TNYPPGGPTAFYWTSDVDTHFILFTME---GIQVKWDKAMSLEIMIPEKYQ----NKVCGLCGNFDLRIDNDMRARSGEI-------------EKDIVNFADSWKVHSSCPV-PQKPPFMCSKRPDRE-----PWAKAKCEILKS-----DSFKKCHDVVN--VEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNN-----ECVERKACECVDPITNLKYNTGDKVIREC 4066
AN Q CICK G + C + G+ HY ++DG F++QG+C YV S+ + + ++N G S + L+ + V G + N P P WT V FT+E G+ V + SL + +P+ + +CGL GN D + +D+ ++G + D + D+W + P + + + D A C ++ +F C + N +E +Y C+ D C + C F ++ + + WR NCP C C + CP +C + C + C++GC C G N +C+ C CVD N V + C
Sbjct: 160 ANHQLNCICKPGYTARRNGRDCDMKVQQACMSGDPHYVTYDGLRFDYQGTCPYVFSQPCTTLPAPYLWYSVRAKNELPGKGYHISQVSEVEVDLHNLTIHVDGRSKTALVNGVQVLTPWYFPNKNTWTVRVRFSGSTFTIENDQGVVVTFTTYNSLCVQVPDIPEFNGATTLCGLAGNIDGKKLDDVVNKNGSVLAIKSSRQPENNNHADFMKTEDTWITDKFLILRPGQENCINGQTLDNNTNCDVATASQTCYPIQQAEMGLGTFGACQGLGNDTLEDFYYNCIYDICR-NPAFKCTEFTYFLQYCQLALPQVAMNQNWRAQINCPLACPLNAHPSTCTSSCPSTCAEPFPEY-----CDQGCVDGCECDPGYVIDNTVTGSIKCIRLDQCGCVDSDGNAHRPGKPWVTQNC 567
BLAST of von Willebrand factor vs. Ensembl Celegans
Match: T01D3.6 (pep chromosome:WBcel235:V:13716849:13720402:1 gene:WBGene00011330.1 transcript:T01D3.6a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T01D3.6) HSP 1 Score: 78.1814 bits (191), Expect = 5.481e-14 Identity = 89/390 (22.82%), Postives = 159/390 (40.77%), Query Frame = 2
Query: 1607 ISGDPHYITFDRRHFFFDGHCEYTAVM--TTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIK-VNFVKISLP-YQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS--KKYREKVT--GMCGNFNGNEMDDS-------MTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKG-------------------ACALSRSYSDYA--NKVCHKLYESE----EFKKCHSAAN--ADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALE----NINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAK---ECVKISDCKC 2629
+SGDPHY+T+D F + G C Y TT L S A + + + SE + D + I++ S+ VN V++ P Y NT + + S ++++ GV + + + + +++ ++ T G+ GN +G ++DD + K + + + KT T +D+ ++ G + +L++S +D A ++ C+ + ++E F C N + F+ C +D+C RN A +C F+ + + C L +N +WR CP CP N + C S C STC C + C++GC C G D +C+++ C C
Sbjct: 190 MSGDPHYVTYDGLRFDYQGTCPYVFSQPCTTLPAPYLWYSVRAKNELPGKGYHISQVSE----------VEVDLHNLTIHVDGRSKTALVNGVQVLTPWYFPNKNTWTVRVRFSGSTFTIENDQGVVVTFTTYNSLCVQVPDIPEFNGATTLCGLAGNIDGKKLDDVVNKNGSVLAIKSSRQPENNNHADFMKTEDTWITDKFLILRPGQENCINGQTLDNNTNCVSTSISLAQSCADVATASQTCYPIQQAEMGLGTFGACQGLGNDTLEDFYYNCIYDIC-----RNPAF---KCTEFTYFLQYCQLALPQVAMNQNWRAQINCPLACP-LNAHPSTCTSSCPSTCAEPFPEYCDQGCVDGCECDPGYVIDNTVTGSIKCIRLDQCGC 560 HSP 2 Score: 61.2326 bits (147), Expect = 6.681e-9 Identity = 89/412 (21.60%), Postives = 147/412 (35.68%), Query Frame = 2
Query: 2963 ANCQNPCICKDGKWVCEQNKQCSGICKTW---GETHYESFDGKYFEFQGSCSYVISED--FCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRN---TNYPPGGPTAFYWTSDVDTHFILFTME---GIQVKWDKAMSLEIMIPEKYQ----NKVCGLCGNFDLRIDNDMRARSGEI-------------EKDIVNFADSWK--------------------------VHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVN--VEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNN-----ECVERKACECVD 4015
AN Q CICK G + C + G+ HY ++DG F++QG+C YV S+ + + ++N G S + L+ + V G + N P P WT V FT+E G+ V + SL + +P+ + +CGL GN D + +D+ ++G + D + D+W V +S + Q + + P +A+ + +F C + N +E +Y C+ D C + C F ++ + + WR NCP C C + CP +C + C + C++GC C G N +C+ C CVD
Sbjct: 160 ANHQLNCICKPGYTARRNGRDCDMKVQQACMSGDPHYVTYDGLRFDYQGTCPYVFSQPCTTLPAPYLWYSVRAKNELPGKGYHISQVSEVEVDLHNLTIHVDGRSKTALVNGVQVLTPWYFPNKNTWTVRVRFSGSTFTIENDQGVVVTFTTYNSLCVQVPDIPEFNGATTLCGLAGNIDGKKLDDVVNKNGSVLAIKSSRQPENNNHADFMKTEDTWITDKFLILRPGQENCINGQTLDNNTNCVSTSISLAQSCADVATASQTCYPIQQAE---MGLGTFGACQGLGNDTLEDFYYNCIYDICR-NPAFKCTEFTYFLQYCQLALPQVAMNQNWRAQINCPLACPLNAHPSTCTSSCPSTCAEPFPEY-----CDQGCVDGCECDPGYVIDNTVTGSIKCIRLDQCGCVD 562
BLAST of von Willebrand factor vs. Ensembl Celegans
Match: apx-1 (Anterior pharynx in excess protein 1 [Source:UniProtKB/Swiss-Prot;Acc:P41990]) HSP 1 Score: 68.1662 bits (165), Expect = 4.046e-11 Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 2
Query: 239 CSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKS 385
CS GW G CS+PIC GGCSN G+CVAPNQC C D ++G C L ++
Sbjct: 163 CSAGWSGEDCSNPICAGGCSNRGRCVAPNQCSCADGFNGTRCEQCLPRA 211
BLAST of von Willebrand factor vs. Ensembl Celegans
Match: ten-1 (Teneurin-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ6]) HSP 1 Score: 64.3142 bits (155), Expect = 9.759e-10 Identity = 25/55 (45.45%), Postives = 32/55 (58.18%), Query Frame = 2
Query: 203 CVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNC 364
CVNG C DG C C GW+G+ C+D C GC + GKC + C C ++G NC
Sbjct: 740 CVNGDCLDDGSCQCWKGWRGSNCTDKKCAIGCEDRGKCASDGSCKCSSGWNGENC 794
BLAST of von Willebrand factor vs. Ensembl Celegans
Match: ten-1 (Teneurin-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ6]) HSP 1 Score: 64.3142 bits (155), Expect = 9.786e-10 Identity = 25/55 (45.45%), Postives = 32/55 (58.18%), Query Frame = 2
Query: 203 CVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNC 364
CVNG C DG C C GW+G+ C+D C GC + GKC + C C ++G NC
Sbjct: 814 CVNGDCLDDGSCQCWKGWRGSNCTDKKCAIGCEDRGKCASDGSCKCSSGWNGENC 868
BLAST of von Willebrand factor vs. Ensembl Fly
Match: Hml (gene:FBgn0029167 transcript:FBtr0333020) HSP 1 Score: 454.907 bits (1169), Expect = 8.255e-130 Identity = 358/1265 (28.30%), Postives = 561/1265 (44.35%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNN----KTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEK--KICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDL-----NVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD--WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGV-IIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTN-ELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLP--YEIR--QDRCNNCTCTNGNWICTSNDCEGKEFCPKS---------MAWFDCGICQ----KTCTNLDLVCPKGQCQLPGCFCPENTVLHNGE--CIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ-FVRGSNPIVSRNTNYPP----------GGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGK-RVWKNCGNICPDSCID--IGKNVTDNTGCKKVCIEGCF---CPDGLHWQNN---ECVERKACECVDPITNLK-YNTGDKVIRE-CEECNC 4081
C+ G ++KTFDG VF PL +TL D + F+ + C+ ++KI Q+++ +N ++ K +P G+ V V ++I ++ + + D D YV + K+ GLCG +G + D VK F ++W D S+L + E C KL QKA+++C+ ++ N C D + C + C C + E+ S C + C KGI ++ WR+ IC C G+ + CG N C + C GC+CP C+ +E CPC E ++ +K NC TC C +G+W+C E++ C RC GDPHY TFD + + F G C Y + T + +V N +A ++ N + C + + + I D I L VN I+ + G +VLI + S+ L ++ G+ + W+G SR+ I R + G+CG FN N DD +T +G E ++ F W+T TC+ + C L+ A K C + + + F+ CH ++F++ C +D C C + C C S+Y +C + + WR + + C KCP + +++CG C +C L + CK EC+EGC CP GE +E ECV C C F G + P E+R + + CTCT+G W C + K+ P S + + C KTC N+D LPGC C E V C+ P C C+ K Y + + I +C N C C+ G W C +N C C WG++H+ +FDG F+FQG+C YV+++ N G F + +N+ CG+ +C+KS++ + + ++ S + N P G AF+ + +QVKWD+ + + + +++ KV GLCGN++ +DM+ S +E + F +WK+ C P P C K P+RE WA+ KC LKSD FK+CH V +E ++KRC+ D C CD+G DC C CTAV Y C + I ++WR+ CP C +K C C D + + + + ++ C+EGC C DG + N+ +CV + C+ V + + K + GD + C C C
Sbjct: 484 CTTWGGINMKTFDGLVFKAPLSCSHTLITDKVSGT------FDIILKACPYGSGYGCAHTLKIL--WQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQID-LESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKL------QKAVSVCERLLANEKLGDCIKPFNYDALIRTCMADYCNC-------ANREHPESCNCDAIAMLAKECAFKGIKLEHGWRN-LEICPI-SCGFGRVYQACGPNVEPTCDSDLALPASKGACNEGCFCPEGTVQYKEACITRELCPCSLRGKE--FKPESTVKKNCNTCTC-KNGQWRCTEDK-CGARCGAVGDPHYQTFDGKRYDFMGKCSYHLLKTQNTSVEAEN--VACSGAVSESMNFAAPDDPSCTKAVTIRFILRDGTPSVIKLDQGLTTIVNDKPIAKLPKMLGLGEVLIRRASSTFLTVEFADGIRVWWDGVSRVYIDAPPSLRGQTQGLCGTFNSNTQDDFLTPEGDVETAVEPFADKWRTKDTCQFKAETHQGPHPCTLNPEKKAQAEKFCDWILQ-DIFQDCHFLVEPEQFYEDCLYDTCAC----KDEMSKCFCPILSAYGTECMRQGVKTGWRMSVKECAVKCPLGQV-FDECGDGCALSCDDLPSKGSCKRECVEGCRCPHGEYVNEDG-ECVPKKMCHCNFDGMSFRPGYKEVRPGEKFLDLCTCTDGVWDCQDAEPGDKDKYPPSSELRSKCAKQPYAEFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSCHHAGKSYDDGEKIKE---DC-NLCECRAGNWKCSKNG-CESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDG-FSITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPIDACKKHPERETWAQLKCGALKSDLFKECHAEVPLERFWKRCIFDTCACDQGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRC 1705 HSP 2 Score: 168.703 bits (426), Expect = 3.343e-41 Identity = 141/491 (28.72%), Postives = 220/491 (44.81%), Query Frame = 2
Query: 1325 EFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGT--MCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGV-IIGEDEDQVAINL---LSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCT-STC-----RLLQESKC-KEECIEGCF---CPEGETF-DEAAKECVKISDCKCEFHGR----FYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
EF++C K C + + S CLPGC C +D + CV C C + +Y G ++K +C C C G W+C +N CE C + GD H+ TFD F F G C+Y DN SITIQN C T C +++ + + G E+ + ++ S+ K K+ G+ I + ++ +++ W+ G+R+ +KL ++R+KV+G+CGN+NGN +DD T E F +WK P C + ++ AC +A C L +S+ FK+CH+ ++F+ +C D C C G C C A ++YA CA + IN+ WR+ CP +C Y C C TC + + E C +E C+EGC C +G + ++ ++CV ++CK R FY D C C C+ IC+ C+
Sbjct: 1247 EFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSC--HHAGKSYDDGEKIKEDCNLCEC-RAGNWKCSKNG-CESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNG-------DGFSITIQNVLCGTMGVT-CSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPID---ACKKHPERETWAQLKCGAL-KSDLFKECHAEVPLERFWKRCIFDTCACD---QGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRCSKRKEICSGVKCD 1718 HSP 3 Score: 146.362 bits (368), Expect = 1.850e-34 Identity = 207/852 (24.30%), Postives = 323/852 (37.91%), Query Frame = 2
Query: 467 IYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCS--ISMKITVGTQTIILFPK-----NEFALVKNNKTKIPFNSEGIIVSSVG-SFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWID-RDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLD-MCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD---WRSATTI---CKQP----QCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSET----------GCPRTIGVIIGEDEDQVAINLLSSSEIKV---NFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKL---SKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSY---SDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCG-SRCTSTCRLLQE--------------SKCKEECI-----EGCFCPEGETFDEAAKECV-KISDCKCEFHGRFYLPYE-IRQDRCNNCTC-TNGNWICTSNDCEGKE-FCPKSMAWFDCGICQKTCTNLDLVCPKGQC 2833
I G TFDG VF Y + L D ++ V+ S +T+ C I+++ T +IL P N + P +VS G + + + + D + V + + GLCGY+NG DD R + I N + F +SW D R P D C LK Q KAL LC + R C V ++L+ C + C C+ N C C I E + C V WR+ C P C+ + C + G +C L C PGCYCP G CVP +C + C+C G + Y+T+DR+ F F+G+C Y LLS + P T Q D+ + C +++ ++ G+ V ++V F +PY+ + + L V +++ E+ VE+ + I L S KY K+ G+CG+ NGN +D + +D + SW+ + E G+VE + + + + C + Y +E F KC A + + C D+C G + G C A ++YA++C I +WR +LCP +CP+ +M Y CG ++ T + L E K E C+ EGCFCP G+ D +CV +I+ KC+ +LP E ++D+C C C + G C C+ +E C + G +T ++D CP+ +C
Sbjct: 2704 ISGKSFTTFDGTVFKYG-PCSHILARDIHSSSWSISVHQQCS-----DETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWYDKRIPKDQ----------CGDLKCPREMQ-AKALQLCNIIHHPTFAR-CHKAVNYKQFLNNYCLEAACNCMMAN--NGDPAACK---CNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVL------PDACFPGCYCPEGTVRKGPNCVPISEC---------------------KDCVCNSLGASK------------------YMTYDRKSFSFNGNCTY----------LLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQRVPQKPKSLQVLVDGFEVKKIPYKDSWIS--LRQVVGKELVLSLPESHVELTASFEDLI-FSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVD-VIQSWQAD---EPKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDICKPGNTQQGV-----CVALAAYAKECNQHGICTNWRRPQLCPYECPS-DMVYEPCGCAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMD--GGQCVPEIACTKCD--DGLHLPDEKWKKDKCTECQCDSKGKTTCVEKKCQVEENICAE-------GYRPETIVSVDECCPRYRC 3453 HSP 4 Score: 118.627 bits (296), Expect = 5.055e-26 Identity = 100/373 (26.81%), Postives = 160/373 (42.90%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQF--VRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHF-ILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPF---MCSKRPDREPWAKAKCE-ILKSDSFKKCHDVVNVEHYYKRCVADACEC-DRGDDCVCFCTAVEQYVARCNEYEIGLK--WRTNENCPHTCTGKRVWKNCG-NICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTK 4108
+C TWG + ++FDG F+ SCS+ + D GTF +I + G S C ++K ++ + ++ F + G+ + + P V H I G+++ WD + + + KV GLCG D + D+ +R+G+ + FAD+W+V C V M S + A + CE +L ++ C N + + C+AD C C +R C C A+ C I L+ WR E CP +C RV++ CG N+ P D+ + K C EGCFCP+G C+ R+ C C + ++ V + C C C G +RCT+
Sbjct: 482 ALCTTWGGINMKTFDGLVFKAPLSCSHTLITDKVS---GTFDIILKACPYG-SGYGCAHTLKILWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKLQKAVSVCERLLANEKLGDCIKPFNYDALIRTCMADYCNCANREHPESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCDSDLALPAS-----KGACNEGCFCPEGTVQYKEACITRELCPCS--LRGKEFKPESTVKKNCNTCTCKNGQWRCTE 843 HSP 5 Score: 100.523 bits (249), Expect = 1.699e-20 Identity = 91/386 (23.58%), Postives = 161/386 (41.71%), Query Frame = 2
Query: 1508 QGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKV-----LIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYK-GACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQ-CTHDVCTCGMKRNGALDHCECEAFSSYARQCALEN--INL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKC---KEECIEGCFCPEGETFDEAAKECVKISDCK 2626
+ Y ++L+ G ++ C+ + C ISG + TFD F + G C + +L + + SI++ C D + C + I + + + L+ +K+ F Q N+ + ++ Q +L + ++ G + + +++ +S K+ V G+CG +NGN+ DD + G + +KF SW D+ I + + G R A ++C+ ++ F +CH A N +F + C C C M NG C+C S+ ++C N + L +WR C CP+ + + RC +C + C + C GC+CPEG CV IS+CK
Sbjct: 2671 EKYDVELDMSASKVGDCLRYSCVLRPNKDDVCEISGK-SFTTFDGTVFKY-GPCSH---------ILARDIHSSSWSISVHQ-QCSDETRKVCHKVITI----QDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSW-------YDKRIPKDQCGDLKCPREMQAKALQLCNIIHHP-TFARCHKAVNYKQFLNNYCLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDACFPGCYCPEGTV--RKGPNCVPISECK 3030 HSP 6 Score: 84.7297 bits (208), Expect = 1.033e-15 Identity = 115/493 (23.33%), Postives = 178/493 (36.11%), Query Frame = 2
Query: 2747 CPKSMAWFDC--GICQKTCTNL---DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXX-------XCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGG------PTAFYWTS--DVDTHFILFTMEGIQVKWDKA---MSLEIMIPE-KYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS-------CPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDI------------GKNVTDNTGCKKVCI-----EGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNC 4081
CP + DC C+ +C N+ D C PGC+CPE TV C+ EC K C +C + G + Y ++D K F F G+C+Y++S D + TF++ C SC KS+ + + V R P ++ G P W S V ++ ++ V+ + + + +P KY +K+ GLCG+ + ND++ + +K V+ SW+ C P C P P + ++ F KC V+ Y C D C+ G+ C A+ Y CN++ I WR + CP+ C V++ CG C +C I + V ++C+ EGCFCP G +CV AC D +L K +C EC C
Sbjct: 2973 CPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDAC-FPGCYCPEGTVRKGPNCVPISEC--------------------------------------KDC--VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQR--VPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAK-KKAGVDVIQSWQADEPKLGLVEECLSEDVPKEHCIPLP---PEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDICK--PGNTQQGVCVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCG--CAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGLHLPDEKWKK--DKCTECQC 3412 HSP 7 Score: 83.5741 bits (205), Expect = 1.891e-15 Identity = 95/361 (26.32%), Postives = 148/361 (41.00%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACG-XXXXXCTKSI---------KFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKA--KCEILKSDSFKKCHDVVNVEHYYKR-CVADACEC---DRGDDCVCFCTAVEQYVARC---NEYEIGLKWRTNENCPHTCTGKRVWKNC-GNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV-DPITNLKYNTGDK 4051
+C+ G++ + +FDG F++ G CS++++ D + IS + C + C K I + I L + G V + N P +F + T + T G V+ D +++ I K+ V GLCG ++ +D R+ G+I + F DSW P Q C RE AKA C I+ +F +CH VN + + C+ AC C + GD C C +E +V +C N WR C C V +C C SC ++ + D C GC+CP+G + CV C +CV + + KY T D+
Sbjct: 2701 VCEISGKS-FTTFDGTVFKY-GPCSHILARDIHSSSWS----ISVHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICK-ASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSW-YDKRIPKDQCGDLKCP----REMQAKALQLCNIIHHPTFARCHKAVNYKQFLNNYCLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGD--DCPVLPDACFPGCYCPEGTVRKGPNCVPISECKDCVCNSLGASKYMTYDR 3047
BLAST of von Willebrand factor vs. Ensembl Fly
Match: Hml (gene:FBgn0029167 transcript:FBtr0075753) HSP 1 Score: 454.521 bits (1168), Expect = 1.032e-129 Identity = 358/1265 (28.30%), Postives = 561/1265 (44.35%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNN----KTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEK--KICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDL-----NVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD--WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGV-IIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTN-ELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLP--YEIR--QDRCNNCTCTNGNWICTSNDCEGKEFCPKS---------MAWFDCGICQ----KTCTNLDLVCPKGQCQLPGCFCPENTVLHNGE--CIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ-FVRGSNPIVSRNTNYPP----------GGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGK-RVWKNCGNICPDSCID--IGKNVTDNTGCKKVCIEGCF---CPDGLHWQNN---ECVERKACECVDPITNLK-YNTGDKVIRE-CEECNC 4081
C+ G ++KTFDG VF PL +TL D + F+ + C+ ++KI Q+++ +N ++ K +P G+ V V ++I ++ + + D D YV + K+ GLCG +G + D VK F ++W D S+L + E C KL QKA+++C+ ++ N C D + C + C C + E+ S C + C KGI ++ WR+ IC C G+ + CG N C + C GC+CP C+ +E CPC E ++ +K NC TC C +G+W+C E++ C RC GDPHY TFD + + F G C Y + T + +V N +A ++ N + C + + + I D I L VN I+ + G +VLI + S+ L ++ G+ + W+G SR+ I R + G+CG FN N DD +T +G E ++ F W+T TC+ + C L+ A K C + + + F+ CH ++F++ C +D C C + C C S+Y +C + + WR + + C KCP + +++CG C +C L + CK EC+EGC CP GE +E ECV C C F G + P E+R + + CTCT+G W C + K+ P S + + C KTC N+D LPGC C E V C+ P C C+ K Y + + I +C N C C+ G W C +N C C WG++H+ +FDG F+FQG+C YV+++ N G F + +N+ CG+ +C+KS++ + + ++ S + N P G AF+ + +QVKWD+ + + + +++ KV GLCGN++ +DM+ S +E + F +WK+ C P P C K P+RE WA+ KC LKSD FK+CH V +E ++KRC+ D C CD+G DC C CTAV Y C + I ++WR+ CP C +K C C D + + + + ++ C+EGC C DG + N+ +CV + C+ V + + K + GD + C C C
Sbjct: 485 CTTWGGINMKTFDGLVFKAPLSCSHTLITDKVSGT------FDIILKACPYGSGYGCAHTLKIL--WQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQID-LESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKL------QKAVSVCERLLANEKLGDCIKPFNYDALIRTCMADYCNC-------ANREHPESCNCDAIAMLAKECAFKGIKLEHGWRN-LEICPI-SCGFGRVYQACGPNVEPTCDSDLALPASKGACNEGCFCPEGTVQYKEACITRELCPCSLRGKE--FKPESTVKKNCNTCTC-KNGQWRCTEDK-CGARCGAVGDPHYQTFDGKRYDFMGKCSYHLLKTQNTSVEAEN--VACSGAVSESMNFAAPDDPSCTKAVTIRFILRDGTPSVIKLDQGLTTIVNDKPIAKLPKMLGLGEVLIRRASSTFLTVEFADGIRVWWDGVSRVYIDAPPSLRGQTQGLCGTFNSNTQDDFLTPEGDVETAVEPFADKWRTKDTCQFKAETHQGPHPCTLNPEKKAQAEKFCDWILQ-DIFQDCHFLVEPEQFYEDCLYDTCAC----KDEMSKCFCPILSAYGTECMRQGVKTGWRMSVKECAVKCPLGQV-FDECGDGCALSCDDLPSKGSCKRECVEGCRCPHGEYVNEDG-ECVPKKMCHCNFDGMSFRPGYKEVRPGEKFLDLCTCTDGVWDCQDAEPGDKDKYPPSSELRSKCAKQPYAEFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSCHHAGKSYDDGEKIKE---DC-NLCECRAGNWKCSKNG-CESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDG-FSITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPIDACKKHPERETWAQLKCGALKSDLFKECHAEVPLERFWKRCIFDTCACDQGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRC 1706 HSP 2 Score: 168.318 bits (425), Expect = 3.793e-41 Identity = 141/491 (28.72%), Postives = 220/491 (44.81%), Query Frame = 2
Query: 1325 EFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGT--MCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGV-IIGEDEDQVAINL---LSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCT-STC-----RLLQESKC-KEECIEGCF---CPEGETF-DEAAKECVKISDCKCEFHGR----FYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
EF++C K C + + S CLPGC C +D + CV C C + +Y G ++K +C C C G W+C +N CE C + GD H+ TFD F F G C+Y DN SITIQN C T C +++ + + G E+ + ++ S+ K K+ G+ I + ++ +++ W+ G+R+ +KL ++R+KV+G+CGN+NGN +DD T E F +WK P C + ++ AC +A C L +S+ FK+CH+ ++F+ +C D C C G C C A ++YA CA + IN+ WR+ CP +C Y C C TC + + E C +E C+EGC C +G + ++ ++CV ++CK R FY D C C C+ IC+ C+
Sbjct: 1248 EFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSC--HHAGKSYDDGEKIKEDCNLCEC-RAGNWKCSKNG-CESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNG-------DGFSITIQNVLCGTMGVT-CSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPID---ACKKHPERETWAQLKCGAL-KSDLFKECHAEVPLERFWKRCIFDTCACD---QGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRCSKRKEICSGVKCD 1719 HSP 3 Score: 146.362 bits (368), Expect = 2.029e-34 Identity = 207/852 (24.30%), Postives = 323/852 (37.91%), Query Frame = 2
Query: 467 IYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCS--ISMKITVGTQTIILFPK-----NEFALVKNNKTKIPFNSEGIIVSSVG-SFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWID-RDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLD-MCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD---WRSATTI---CKQP----QCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSET----------GCPRTIGVIIGEDEDQVAINLLSSSEIKV---NFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKL---SKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSY---SDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCG-SRCTSTCRLLQE--------------SKCKEECI-----EGCFCPEGETFDEAAKECV-KISDCKCEFHGRFYLPYE-IRQDRCNNCTC-TNGNWICTSNDCEGKE-FCPKSMAWFDCGICQKTCTNLDLVCPKGQC 2833
I G TFDG VF Y + L D ++ V+ S +T+ C I+++ T +IL P N + P +VS G + + + + D + V + + GLCGY+NG DD R + I N + F +SW D R P D C LK Q KAL LC + R C V ++L+ C + C C+ N C C I E + C V WR+ C P C+ + C + G +C L C PGCYCP G CVP +C + C+C G + Y+T+DR+ F F+G+C Y LLS + P T Q D+ + C +++ ++ G+ V ++V F +PY+ + + L V +++ E+ VE+ + I L S KY K+ G+CG+ NGN +D + +D + SW+ + E G+VE + + + + C + Y +E F KC A + + C D+C G + G C A ++YA++C I +WR +LCP +CP+ +M Y CG ++ T + L E K E C+ EGCFCP G+ D +CV +I+ KC+ +LP E ++D+C C C + G C C+ +E C + G +T ++D CP+ +C
Sbjct: 2705 ISGKSFTTFDGTVFKYG-PCSHILARDIHSSSWSISVHQQCS-----DETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWYDKRIPKDQ----------CGDLKCPREMQ-AKALQLCNIIHHPTFAR-CHKAVNYKQFLNNYCLEAACNCMMAN--NGDPAACK---CNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVL------PDACFPGCYCPEGTVRKGPNCVPISEC---------------------KDCVCNSLGASK------------------YMTYDRKSFSFNGNCTY----------LLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQRVPQKPKSLQVLVDGFEVKKIPYKDSWIS--LRQVVGKELVLSLPESHVELTASFEDLI-FSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVD-VIQSWQAD---EPKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDICKPGNTQQGV-----CVALAAYAKECNQHGICTNWRRPQLCPYECPS-DMVYEPCGCAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMD--GGQCVPEIACTKCD--DGLHLPDEKWKKDKCTECQCDSKGKTTCVEKKCQVEENICAE-------GYRPETIVSVDECCPRYRC 3454 HSP 4 Score: 118.627 bits (296), Expect = 5.184e-26 Identity = 100/373 (26.81%), Postives = 160/373 (42.90%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQF--VRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHF-ILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPF---MCSKRPDREPWAKAKCE-ILKSDSFKKCHDVVNVEHYYKRCVADACEC-DRGDDCVCFCTAVEQYVARCNEYEIGLK--WRTNENCPHTCTGKRVWKNCG-NICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTK 4108
+C TWG + ++FDG F+ SCS+ + D GTF +I + G S C ++K ++ + ++ F + G+ + + P V H I G+++ WD + + + KV GLCG D + D+ +R+G+ + FAD+W+V C V M S + A + CE +L ++ C N + + C+AD C C +R C C A+ C I L+ WR E CP +C RV++ CG N+ P D+ + K C EGCFCP+G C+ R+ C C + ++ V + C C C G +RCT+
Sbjct: 483 ALCTTWGGINMKTFDGLVFKAPLSCSHTLITDKVS---GTFDIILKACPYG-SGYGCAHTLKILWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKLQKAVSVCERLLANEKLGDCIKPFNYDALIRTCMADYCNCANREHPESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCDSDLALPAS-----KGACNEGCFCPEGTVQYKEACITRELCPCS--LRGKEFKPESTVKKNCNTCTCKNGQWRCTE 844 HSP 5 Score: 100.138 bits (248), Expect = 1.895e-20 Identity = 91/386 (23.58%), Postives = 161/386 (41.71%), Query Frame = 2
Query: 1508 QGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKV-----LIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYK-GACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQ-CTHDVCTCGMKRNGALDHCECEAFSSYARQCALEN--INL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKC---KEECIEGCFCPEGETFDEAAKECVKISDCK 2626
+ Y ++L+ G ++ C+ + C ISG + TFD F + G C + +L + + SI++ C D + C + I + + + L+ +K+ F Q N+ + ++ Q +L + ++ G + + +++ +S K+ V G+CG +NGN+ DD + G + +KF SW D+ I + + G R A ++C+ ++ F +CH A N +F + C C C M NG C+C S+ ++C N + L +WR C CP+ + + RC +C + C + C GC+CPEG CV IS+CK
Sbjct: 2672 EKYDVELDMSASKVGDCLRYSCVLRPNKDDVCEISGK-SFTTFDGTVFKY-GPCSH---------ILARDIHSSSWSISVHQ-QCSDETRKVCHKVITI----QDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSW-------YDKRIPKDQCGDLKCPREMQAKALQLCNIIHHP-TFARCHKAVNYKQFLNNYCLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDACFPGCYCPEGTV--RKGPNCVPISECK 3031 HSP 6 Score: 84.3445 bits (207), Expect = 1.077e-15 Identity = 115/493 (23.33%), Postives = 178/493 (36.11%), Query Frame = 2
Query: 2747 CPKSMAWFDC--GICQKTCTNL---DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXX-------XCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGG------PTAFYWTS--DVDTHFILFTMEGIQVKWDKA---MSLEIMIPE-KYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS-------CPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDI------------GKNVTDNTGCKKVCI-----EGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNC 4081
CP + DC C+ +C N+ D C PGC+CPE TV C+ EC K C +C + G + Y ++D K F F G+C+Y++S D + TF++ C SC KS+ + + V R P ++ G P W S V ++ ++ V+ + + + +P KY +K+ GLCG+ + ND++ + +K V+ SW+ C P C P P + ++ F KC V+ Y C D C+ G+ C A+ Y CN++ I WR + CP+ C V++ CG C +C I + V ++C+ EGCFCP G +CV AC D +L K +C EC C
Sbjct: 2974 CPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDAC-FPGCYCPEGTVRKGPNCVPISEC--------------------------------------KDC--VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQR--VPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAK-KKAGVDVIQSWQADEPKLGLVEECLSEDVPKEHCIPLP---PEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDICK--PGNTQQGVCVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCG--CAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGLHLPDEKWKK--DKCTECQC 3413 HSP 7 Score: 83.5741 bits (205), Expect = 1.907e-15 Identity = 95/361 (26.32%), Postives = 148/361 (41.00%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACG-XXXXXCTKSI---------KFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKA--KCEILKSDSFKKCHDVVNVEHYYKR-CVADACEC---DRGDDCVCFCTAVEQYVARC---NEYEIGLKWRTNENCPHTCTGKRVWKNC-GNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV-DPITNLKYNTGDK 4051
+C+ G++ + +FDG F++ G CS++++ D + IS + C + C K I + I L + G V + N P +F + T + T G V+ D +++ I K+ V GLCG ++ +D R+ G+I + F DSW P Q C RE AKA C I+ +F +CH VN + + C+ AC C + GD C C +E +V +C N WR C C V +C C SC ++ + D C GC+CP+G + CV C +CV + + KY T D+
Sbjct: 2702 VCEISGKS-FTTFDGTVFKY-GPCSHILARDIHSSSWS----ISVHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICK-ASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSW-YDKRIPKDQCGDLKCP----REMQAKALQLCNIIHHPTFARCHKAVNYKQFLNNYCLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGD--DCPVLPDACFPGCYCPEGTVRKGPNCVPISECKDCVCNSLGASKYMTYDR 3048
BLAST of von Willebrand factor vs. Ensembl Fly
Match: cv-2 (gene:FBgn0000395 transcript:FBtr0071610) HSP 1 Score: 127.487 bits (319), Expect = 2.967e-29 Identity = 109/421 (25.89%), Postives = 172/421 (40.86%), Query Frame = 2
Query: 2624 KCEFHGRFYL-PYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIK-----------PFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLN-RVFQFVRGSNPIVSRNTNYP----PGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKD--IVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCP 3823
KC F+ Y + DRC NCTC NG +C C E P+ C C + + +C L G N G C CP N+ + + P C C++ + +G C +G+ H+ +FDGK+F FQGSC Y+++ D G TF + N G+ +S K++ N +V R + P GG +L + G+ ++W+ A L++ +P K++ ++CGLCGNF+ +D+ + G D + +FA+SWKV +K F+ + + + C L + F +C++ +N E+Y C D CEC GD C C + Y C + L WR+ NCP
Sbjct: 255 KCLFNKSVYPEKTQFMPDRCTNCTCLNGTSVCQRPTCPILECAPEFQEPDGC------CPRCAVAEVRSECSLDGIVYQNNETWDMGPCRSCRCNGGTIRCAQMRCPAVK----CRANEELKQPPGEC------------CQRCVETAGTCTVFGDPHFRTFDGKFFSFQGSCKYLLASDCMGK---TFHIRLTNEGRGTRRASWAKTVTLSLRNLKVNLGQRMRVKVNGTRVTLPYFVVAGGQNVTIERLANGGAVMLRSEMGLTLEWNGAGFLQVSVPAKFKKRLCGLCGNFNGSSRDDLTGKDGRSHGDDEVWHFANSWKVGGPKSCSRKREFLAATPTCDKRKSNFYCHPLSVPALFGECNERLNPENYKAACRMDVCECPSGD---CHCDSFAAYAHECRRLGVQLPDWRSATNCP 647 HSP 2 Score: 124.02 bits (310), Expect = 3.705e-28 Identity = 109/413 (26.39%), Postives = 171/413 (41.40%), Query Frame = 2
Query: 1280 SATTICKQPQCFN---GQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWD-----------GTMCVPQEKCPCV--RDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPY-QHTGNTKVLIDQVSTD-ILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKD--IDKFVLSWKTN--PTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCP 2449
+ T++C++P C EF E C C + EC L G N WD GT+ Q +CP V R N E G C+ C+ E G C + GDPH+ TFD + F F G C+Y +L S+ I + N + +T+ + + + +NL +KVN +++LPY G V I++++ + L+SE G+ + WNG +++ + K+++++ G+CGNFNG+ DD G D + F SWK +C R + C +S N CH L F +C+ N + + C DVC C C C++F++YA +C + L WR+ CP
Sbjct: 281 NGTSVCQRPTCPILECAPEFQE-PDGCCPRCAVAEVRSECS---LDGIVYQNNETWDMGPCRSCRCNGGTIRCAQMRCPAVKCRANEELKQPPGE----CCQRCV-------------ETAGTCTVFGDPHFRTFDGKFFSFQGSCKY---------LLASDCMGKTFHIRLTNEG-RGTRRASWAKTVTLSL----RNLKVNLGQRMRVKVNGTRVTLPYFVVAGGQNVTIERLANGGAVMLRSEMGLTLEWNGAGFLQVSVPAKFKKRLCGLCGNFNGSSRDDLTGKDGRSHGDDEVWHFANSWKVGGPKSCSRKREFLAATPTCDKRKS-----NFYCHPLSVPALFGECNERLNPENYKAACRMDVCEC------PSGDCHCDSFAAYAHECRRLGVQLPDWRSATNCP 647 HSP 3 Score: 72.0182 bits (175), Expect = 4.944e-12 Identity = 65/291 (22.34%), Postives = 113/291 (38.83%), Query Frame = 2
Query: 446 TYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTI-ILFPKNEFALVKNNKTKIPF----NSEGIIVSSV--GSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDY--RDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSAT 1288
T C+V H +TFDG FS+ Y L DC+G F++ + + S + +T+ + + + + V + +P+ + + + + G + ++ L + ++ + V P +K++CGLCG FNG DD +D V F NSW P + RE L +K+ C + L C + + Y C+ ++CEC +C+ C Y++ C G+ + DWRSAT
Sbjct: 379 TAGTCTVFGDPHFRTFDGKFFSFQGSCKYLLASDCMGKT--FHIRLTN-----EGRGTRRASWAKTVTLSLRNLKVNLGQRMRVKVNGTRVTLPYFVVAGGQNVTIERLANGGAVMLRSEMGLTLEWNGAGFLQVSVPAKFKKRLCGLCGNFNGSSRDDLTGKDGRSHGDDEVWHFANSWKVGGPKSCSRKRE-------FLAATPTCDKRKSNFYCHPLSVPALFGECNERLNPENYKAACRMDVCECPSG--------DCH---CDSFAAYAHECRRLGVQLPDWRSAT 644
BLAST of von Willebrand factor vs. Ensembl Fly
Match: Ten-a (gene:FBgn0267001 transcript:FBtr0300201) HSP 1 Score: 64.6994 bits (156), Expect = 1.107e-9 Identity = 34/109 (31.19%), Postives = 55/109 (50.46%), Query Frame = 2
Query: 191 CSPDCV-NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPN---QCLCLDNYSGPNCNTLLIKSSQSDVSNYG-AVTRVNNTMYSTYYECSVIYGHHIKTFDGN 502
C C +G CK+G C CS GW G C+ P C GCS G+C N +C C++ ++G +C+ L + + ++ N G +T +++ ++ CS HI N
Sbjct: 655 CDSRCSEHGQCKNGTCVCSQGWNGRHCTLPGCENGCSRHGQCTLENGEYRCDCIEGWAGRDCSIALELNCKDNIDNDGDGMTDCSDSECCSHPACS----EHIMCLSSN 759 HSP 2 Score: 60.077 bits (144), Expect = 2.458e-8 Identity = 33/96 (34.38%), Postives = 45/96 (46.88%), Query Frame = 2
Query: 191 CSPDC-VNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYG 475
CS DC NGVC+ G+C C+ GW GN C C CS G+C C+C ++G +C + ++ S +G T N Y C I G
Sbjct: 624 CSLDCGRNGVCESGKCRCNSGWTGNLCDQLPCDSRCSEHGQC-KNGTCVCSQGWNGRHCT---LPGCENGCSRHGQCTLENGE-----YRCDCIEG 710
BLAST of von Willebrand factor vs. Ensembl Fly
Match: Ten-a (gene:FBgn0267001 transcript:FBtr0308221) HSP 1 Score: 64.6994 bits (156), Expect = 1.120e-9 Identity = 34/109 (31.19%), Postives = 55/109 (50.46%), Query Frame = 2
Query: 191 CSPDCV-NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPN---QCLCLDNYSGPNCNTLLIKSSQSDVSNYG-AVTRVNNTMYSTYYECSVIYGHHIKTFDGN 502
C C +G CK+G C CS GW G C+ P C GCS G+C N +C C++ ++G +C+ L + + ++ N G +T +++ ++ CS HI N
Sbjct: 757 CDSRCSEHGQCKNGTCVCSQGWNGRHCTLPGCENGCSRHGQCTLENGEYRCDCIEGWAGRDCSIALELNCKDNIDNDGDGMTDCSDSECCSHPACS----EHIMCLSSN 861 HSP 2 Score: 60.077 bits (144), Expect = 2.508e-8 Identity = 33/96 (34.38%), Postives = 45/96 (46.88%), Query Frame = 2
Query: 191 CSPDC-VNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYG 475
CS DC NGVC+ G+C C+ GW GN C C CS G+C C+C ++G +C + ++ S +G T N Y C I G
Sbjct: 726 CSLDCGRNGVCESGKCRCNSGWTGNLCDQLPCDSRCSEHGQC-KNGTCVCSQGWNGRHCT---LPGCENGCSRHGQCTLENGE-----YRCDCIEG 812
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Match: vwf (von Willebrand factor [Source:ZFIN;Acc:ZDB-GENE-070103-1]) HSP 1 Score: 529.25 bits (1362), Expect = 5.020e-154 Identity = 361/1269 (28.45%), Postives = 572/1269 (45.07%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGID-DLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEF------------------CPKSMAWFDC------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQF----VRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC------TGKRV-WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACE-CVDPITNLKYNTGDKVI------RECEECNCYEGAFRC 4102
CS+ HI TFDG ++ +P Y L DC + F+ D K + + + +G + + + +P+ S + ++++ + I D NI + + CGLCG +N + D+Y + + F NSW + + CN+ DQ ++ C+ M + L C V ++ +C+ ++C C S + C C+ +YS AC +GI + +W + + P+C G E+S+C C +C + ++ C+ +C+ GC CP + DG CV +C C + Y + +C TC+C HG W+C NE C G C ++G HY TFD + F F G C Y +L + S I+ C D+ + C R+I + + +Q ++L + V+ + + P N + I +RL+ + + W+G R+ ++L Y ++ G+CGN+NGN+ DD +T G+ E ++ F SWK N C+ + ++ C+L+ +A + C L S +F+ CH N + + C +DVC C + C C+A SSYA CA + ++WR++ C CP+ + Y CGS C TCR L ++ C C EGCFCP G F A ECV C C +G + P ++ D + C C +G+ CTSN+ G CP + F+C GI C KTC NL+L C C + GC CP TV + +CI +CPCY N++ Y +I +C N C+C+ +W C + + C G+C+ G+THY SFDG F F G C YV+++D+C GTF+++ EN ACG + C+KSI +Y+ + VR P++ ++I+ + I + WD + + I +Y+ KVCGLCGNFD +ND+ + S ++E D +F +SWKV SC CS + + C +L S F+ C+ VV+ E Y+ C D C C DC CFC + Y C + + + WR+N+ CP +C +G + W+ C + CP +C + C C+EGC CP G L +CVE C+ CV + + G + I R C+ C+C C
Sbjct: 28 RCSLFSRQHIHTFDGVIYEFPGDCSYMLAGDCRQRS------FSILGDFSHGKRKG-----VTVFLGEMFELHLSVDGTLSQGAERLPLPYASSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKDQ----MSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHC-------SAGKEC---VCQAMLEYSRACASRGITLSNWHTQSQC--SPKCPVGMEYSDCTPTCSTSCQNVNIQEVCKEECVDGCICPAGKVLDGDRCVDVSQCSCT--HAGRRYPPASYISQDCNTCVC-RHGSWECT-NEGCPGECLVTGQSHYKTFDNKFFTFSGICHY---------LLAKDCQSNSFSAVIETAQCADDQDAICTRSITLRFKDLANQT-VHLKHGGVVSVDGMDVQTPLI---NGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYAGQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSWKMNADCDDVQ--MQNSDPCSLNPKRVRFAEEACAVLL-SMKFEPCHYMVNPEPYVKNCRYDVCACADGQE-----CLCDAVSSYAVACAARGVLVNWRSHGYCELTCPDAQV-YEMCGSVCNQTCRSLSLPDTGCDGVCEEGCFCPRG-LFLSDAGECVPPQHCSCHHNGEIFEPNDMIGDHHSICVCESGSMHCTSNEATGSTLSDLFFDEQSPSRGRRSLSCPPPLQRFECESARDAGIECAKTCQNLELDCVSLGC-VSGCMCPPGTVRYRKDCIPQEQCPCYHNSRPYESGQSIT---VDC-NTCVCEQRQWKCSE-RVCDGVCRAIGDTHYISFDGLKFSFPGPCQYVLAQDYCNGLDGTFRVLVENSACGIAGYRCSKSITVLYMGGLIVMEHGEVRMKRPVLKETE----------VEIVRSGLYYIVLLGKHISITWDTGTRISLQISGQYRGKVCGLCGNFDGSQNNDLLSSSNQMEVDAADFGNSWKVRPSCADAVSVVSQCSTDLVKLVTVEQSCRVLTSAIFRDCNSVVDPEPYWDICTQDTCSCPSVGDCACFCNTIAAYAHECAQKGLPVNWRSNDLCPLSCEDLNKESGVQCEWRYNTCASACPSTC-----QHPEPLSCPISCVEGCHAVCPPGHLLDEVLRKCVEPSQCQVCVH--DGQRVSHGKQFILNHEDPRLCQICHCESNTLTC 1219 HSP 2 Score: 141.354 bits (355), Expect = 7.107e-33 Identity = 107/388 (27.58%), Postives = 158/388 (40.72%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSET-GVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK 2740
E RC + H TFD + F G C Y M D S S + S G + + V +GE ++L + ++ LPY + + +RL SE G I + I++ L K++ + G+CGN+N D+ +G D F SW E + ++ +C + + C + S F C + F C DVC C + C C+A Y+R CA I LS W T C KCP M+Y+ C C+++C+ + + CKEEC++GC CP G+ D CV +S C C GR Y P CN C C +G+W CT+ C G+
Sbjct: 24 EGSARCSLFSRQHIHTFDGVIYEFPGDCSY---MLAGDCRQRSFSILGDFS-------------HGKRKGVTVFLGE---MFELHLSVDGTLSQGAERLPLPYASSS----VFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKDQMSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHCSAGKE-----CVCQAMLEYSRACASRGITLSNWHTQSQCSPKCP-VGMEYSDCTPTCSTSCQNVNIQEVCKEECVDGCICPAGKVLD--GDRCVDVSQCSCTHAGRRYPPASYISQDCNTCVCRHGSWECTNEGCPGE 380 HSP 3 Score: 128.642 bits (322), Expect = 6.420e-29 Identity = 95/384 (24.74%), Postives = 168/384 (43.75%), Query Frame = 2
Query: 3029 SGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME--GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT-KLFSKECLHTSRPEIST 4156
S C + H +FDG +EF G CSY+++ D C + +F ++ G S K + ++L +F+ + +S+ P + + + ++ E G ++ D ++++ + +++ N+ CGLCGN++ +++ A+ G + D +FA+SW + + C P C+ + + CE ++S F C +V+ + C +D C C G +CV C A+ +Y C I L W T C C + +C C SC +NV CK+ C++GC CP G + CV+ C C +Y + ++C C C G++ CT + ECL T + T
Sbjct: 26 SARCSLFSRQHIHTFDGVIYEFPGDCSYMLAGD-CRQR--SFSIL------GDFSHGKRKGVT-VFLGEMFELHLSVDGTLSQGAERLPLPYASSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKDQMSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHCSAGKECV--CQAMLEYSRACASRGITLSNWHTQSQCSPKCPVGMEYSDCTPTCSTSC----QNVNIQEVCKEECVDGCICPAGKVLDGDRCVDVSQCSCTH--AGRRYPPASYISQDCNTCVCRHGSWECTNEGCPGECLVTGQSHYKT 391 HSP 4 Score: 112.849 bits (281), Expect = 3.326e-24 Identity = 116/470 (24.68%), Postives = 191/470 (40.64%), Query Frame = 2
Query: 191 CSPDCVNG-VCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPF-NSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGM-ELECESQCLPGCYCPRNRYW-DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENE 1585
C +CV+G +C G+ G+RC D + C++ G+ P +Y +CNT + + + +N G EC V H KTFD F++ Y L DC + F + DQ SI+++ + V + P N I ++V S ++++ DL + +D R R + P A ++CGLCG +NG DD+ +++ V+ F NSW + +D + + + PC+ N + + A C ++ C V + Y+ C+ ++C C Q C Y+ AC +G+ V+WRS + C + Q + CGS C + C L + + C+ C GC+CPR + D CVP + C C NGE ++ + + C+C G C NE
Sbjct: 314 CKEECVDGCICPAGKV-----LDGDRCVD-VSQCSCTHAGRRYPPA------SYISQDCNTCVCRHGSWECTNEGCPG-----------ECLVTGQSHYKTFDNKFFTFSGICHYLLAKDCQSNS--FSAVIETAQCADDQDAICTRSITLRFKDLANQTVHLKHGGVVSVDGMDVQTPLINGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYA-GQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSW--KMNADCDDVQMQNSDPCSL----NPKRVRFAEEACAVLLSMKF-EPCHYMVNPEPYVKNCRYDVCACADGQ----------ECLCDAVSSYAVACAARGVLVNWRSHGYC--ELTCPDAQVYEMCGSVCNQTCRSLSLPDTGCDGVCEEGCFCPRGLFLSDAGECVPPQHCSC-HHNGE-IFEPNDMIGDHHSICVC-ESGSMHCTSNE 735 HSP 5 Score: 77.0258 bits (188), Expect = 2.864e-13 Identity = 83/380 (21.84%), Postives = 148/380 (38.95%), Query Frame = 2
Query: 2966 NCQN--PCICKDGKWVCEQNKQCSGI------CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIAC-GXXXXXCTKSIKF------IYLNRVFQFVRG----SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCID--------IGKNVTDNTG--CKKVCIEGCFCPDGLHWQNNECVERKAC-ECVD 4015
NC+ P +CK+ N C C +H SF G + + +CSY++ + G +L+ AC S + C KS++ + L + G + P + G S+ + FT + + +++ + GLCG+ + + G + D + +W + S C V + +C ++ C++L S+ F CH +V V+ Y + C + AC+ + C V Y C + + + WRT+ CP +C+ + C + C + C +G + G C EGCFCP+G ++CV+R+AC +CV+
Sbjct: 1919 NCEKMEPPVCKNNLPALRYNHHCGCTWTCPCSCMGSSTSHVVSFSGAALKVKSACSYLLL------RSGGAELVLHTTACQSSPNQICMKSLELREGGTSVVLQDDMKVTVGGVLTAVPFRTERVTVTQIGSILHQLRSEQHGFTLTFTPHSNEFMLNMDIAM--------KPNTTGLCGSCS-DDQSGLLLSDGSVTTDPDVYLQAWALGSEC-VQHQEIQLCPAGLEK------ACDVLSSEVFSHCHALVPVQDYIQICRSVACQPNA------LCDLVTAYSTVCRQQGVCVDWRTSSLCPMSCSDSMEYAACRSGCLEQCGQSQFTPGDMMGDGLLKANGSVCLNTPTEGCFCPEGSVLMEDKCVDREACSQCVE 2270 HSP 6 Score: 72.7886 bits (177), Expect = 4.666e-12 Identity = 106/475 (22.32%), Postives = 177/475 (37.26%), Query Frame = 2
Query: 1589 CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSET-GVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ---------------ESKCKEECIEGCFCPEGETFDEAAKECVKISDC-KCEFHG----RFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQKTCTNL-DLVCPKGQC---------QLPGCFCPENTVLHN-GECIKPFECPC 2911
C C S H ++F C Y LL S A L + T CQ + C +++ + E ++ L ++ V V ++P++ +V + Q+ + + +L+SE G + + S + + + TG+CG+ + ++ + G D D ++ +W C + I K C L SE F CH+ + C C + AL C+ ++Y+ C + + + WRT+ LCP C +++M+Y C S C C Q S C EGCFCPEG E +CV C +C H +F + + C C C + I CT+ C + +CG C+ + CP+ +C P C + VL N G+C+ ++C C
Sbjct: 1947 CPCSCMGSSTSHVVSFSGAALKVKSACSY----------LLLRSGGAEL--VLHTTACQSSPNQICMKSLEL----REGGTSVVLQDDMKVTVGGVLTAVPFR---TERVTVTQIGSILHQLRSEQHGFTLTFTPHSNEFMLNMDIAMKPNTTGLCGSCS-DDQSGLLLSDGSVTTDPDVYLQAWALGSECVQHQEIQLCPAG----------LEKACDVL-SSEVFSHCHALVPVQDYIQICRSVAC-----QPNAL----CDLVTAYSTVCRQQGVCVDWRTSSLCPMSC-SDSMEYAACRSGCLEQCGQSQFTPGDMMGDGLLKANGSVCLNTPTEGCFCPEGSVLME--DKCVDREACSQCVEHSGKTHQFMESWNPEDNPCVLCVCLDQQRINCTALPCTNAKA-------PECGQCEVLQEKMGSKCCPQYECVCDINCKRPDPPHCDHGLSVVLTNPGDCLPVYQCVC 2371 HSP 7 Score: 58.151 bits (139), Expect = 1.358e-7 Identity = 81/377 (21.49%), Postives = 144/377 (38.20%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD--LEILFDRDRNIYVY-APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQL----------GMELECESQCL----PGCYCPRNRYWDGTMCVPQEKCP-CVRDNGES-TYQQGYRLKLN-CETCIC 1549
H+ +F G Y L G A L ++ A +Q C S+++ G +++L + V T +PF +E + V+ +GS + + + F N ++ + + GLCG SDD + + DP +VY + ++ + L D++ + + C + V + Y+ +C+ C QP + C + YS C +G+ VDWR+ +++C C + E++ C S C + CGQ G+ S CL GC+CP CV +E C CV +G++ + + + + N C C+C
Sbjct: 1958 HVVSFSGAALKVKSACSYLLLRS--GGAELV-LHTTACQSSPNQI----CMKSLELREGGTSVVLQDDMKVT-VGGVLTAVPFRTERVTVTQIGSILHQLRSEQHGFTLTFTPHSNEFMLNMDIAMKPNTTGLCGS----CSDDQSGL---------LLSDGSVTTDP---DVYLQAWALGSECVQHQEIQLCPAGLEKACDVLSSEVFSHCHALVPVQDYIQICRSVAC-----QP---------NALCDLVTAYSTVCRQQGVCVDWRT-SSLCPM-SCSDSMEYAACRSGCLEQCGQSQFTPGDMMGDGLLKANGSVCLNTPTEGCFCPEGSVLMEDKCVDREACSQCVEHSGKTHQFMESWNPEDNPCVLCVC 2294
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Match: vwf (von Willebrand factor [Source:ZFIN;Acc:ZDB-GENE-070103-1]) HSP 1 Score: 522.702 bits (1345), Expect = 1.848e-153 Identity = 360/1273 (28.28%), Postives = 570/1273 (44.78%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGID-DLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLK----NNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEF------------------CPKSMAWFDC------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQF----VRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC------TGKR-VWK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACE-CVDPITNLKYNTGDKVI------RECEECNCYEGAFRC 4102
CS+ HI TFDG ++ +P Y L DC + F+ D K + + + +G + + + +P+ S + ++++ + I D NI + + CGLCG +N + D+Y + + F NSW + + PC + N+ ++ C+ M + L C V ++ +C+ ++C C S + C C+ +YS AC +GI + +W + + P+C G E+S+C C +C + ++ C+ +C+ GC CP + DG CV +C C + Y + +C TC+C HG W+C NE C G C ++G HY TFD + F F G C Y N S I+ C D+ + C R+I + + +Q ++L + V+ + + P N + I +RL+ + + W+G R+ ++L Y ++ G+CGN+NGN+ DD +T G+ E ++ F SWK N C+ + ++ C+L+ +A + C L S +F+ CH N + + C +DVC C + C C+A SSYA CA + ++WR++ C CP+ + Y CGS C TCR L ++ C C EGCFCP G F A ECV C C +G + P ++ D + C C +G+ CTSN+ G CP + F+C GI C KTC NL+L C C + GC CP TV + +CI +CPCY N++ Y +I +C N C+C+ +W C + + C G+C+ G+THY SFDG F F G C YV+++D+C GTF+++ EN ACG + C+KSI +Y+ + VR P++ ++I+ + I + WD + + I +Y+ KVCGLCGNFD +ND+ + S ++E D +F +SWKV SC CS + + C +L S F+ C+ VV+ E Y+ C D C C DC CFC + Y C + + + WR+N+ CP +C +G + W+ C + CP +C + C C+EGC CP G L +CVE C+ CV + + G + I R C+ C+C C
Sbjct: 16 RCSLFSRQHIHTFDGVIYEFPGDCSYMLAGDCRQRS------FSILGDFSHGKRKG-----VTVFLGEMFELHLSVDGTLSQGAERLPLPYASSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADE----------PCKRVLPPSQSCNSTGESTKMSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHC-------SAGKEC---VCQAMLEYSRACASRGITLSNWHTQSQC--SPKCPVGMEYSDCTPTCSTSCQNVNIQEVCKEECVDGCICPAGKVLDGDRCVDVSQCSCT--HAGRRYPPASYISQDCNTCVC-RHGSWECT-NEGCPGECLVTGQSHYKTFDNKFFTFSGICHYLLAKDCQSN---------SFSAVIETAQCADDQDAICTRSITLRFKDLANQT-VHLKHGGVVSVDGMDVQTPLI---NGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYAGQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSWKMNADCDDVQ--MQNSDPCSLNPKRVRFAEEACAVLL-SMKFEPCHYMVNPEPYVKNCRYDVCACADGQE-----CLCDAVSSYAVACAARGVLVNWRSHGYCELTCPDAQV-YEMCGSVCNQTCRSLSLPDTGCDGVCEEGCFCPRG-LFLSDAGECVPPQHCSCHHNGEIFEPNDMIGDHHSICVCESGSMHCTSNEATGSTLSDLFFDEQSPSRGRRSLSCPPPLQRFECESARDAGIECAKTCQNLELDCVSLGC-VSGCMCPPGTVRYRKDCIPQEQCPCYHNSRPYESGQSIT---VDC-NTCVCEQRQWKCSE-RVCDGVCRAIGDTHYISFDGLKFSFPGPCQYVLAQDYCNGLDGTFRVLVENSACGIAGYRCSKSITVLYMGGLIVMEHGEVRMKRPVLKETE----------VEIVRSGLYYIVLLGKHISITWDTGTRISLQISGQYRGKVCGLCGNFDGSQNNDLLSSSNQMEVDAADFGNSWKVRPSCADAVSVVSQCSTDLVKLVTVEQSCRVLTSAIFRDCNSVVDPEPYWDICTQDTCSCPSVGDCACFCNTIAAYAHECAQKGLPVNWRSNDLCPLSCEDLNKESGVQCEWRYNTCASACPSTC-----QHPEPLSCPISCVEGCHAVCPPGHLLDEVLRKCVEPSQCQVCVH--DGQRVSHGKQFILNHEDPRLCQICHCESNTLTC 1205 HSP 2 Score: 137.887 bits (346), Expect = 8.524e-32 Identity = 107/388 (27.58%), Postives = 159/388 (40.98%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSET-GVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK 2740
E RC + H TFD + F G C Y M D S S + S G + + V +GE ++L + ++ LPY + + +RL SE G I + I++ L K++ + G+CGN+N D+ +G D F SW E + ++ +C + + + C + S F C + F C DVC C + C C+A Y+R CA I LS W T C KCP M+Y+ C C+++C+ + + CKEEC++GC CP G+ D CV +S C C GR Y P CN C C +G+W CT+ C G+
Sbjct: 12 EGSARCSLFSRQHIHTFDGVIYEFPGDCSY---MLAGDCRQRSFSILGDFS-------------HGKRKGVTVFLGE---MFELHLSVDGTLSQGAERLPLPYASSS----VFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKMS--ACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHCSAGKE-----CVCQAMLEYSRACASRGITLSNWHTQSQCSPKCP-VGMEYSDCTPTCSTSCQNVNIQEVCKEECVDGCICPAGKVLD--GDRCVDVSQCSCTHAGRRYPPASYISQDCNTCVCRHGSWECTNEGCPGE 366 HSP 3 Score: 125.946 bits (315), Expect = 3.220e-28 Identity = 96/382 (25.13%), Postives = 167/382 (43.72%), Query Frame = 2
Query: 3029 SGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME--GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLF-SKECLHTSRPEIST 4156
S C + H +FDG +EF G CSY+++ D C + +F ++ G S K + ++L +F+ + +S+ P + + + ++ E G ++ D ++++ + +++ N+ CGLCGN++ +++ A+ G + D +FA+SW + + P + P S E + CE ++S F C +V+ + C +D C C G +CV C A+ +Y C I L W T C C + +C C SC +NV CK+ C++GC CP G + CV+ C C +Y + ++C C C G++ CT ECL T + T
Sbjct: 14 SARCSLFSRQHIHTFDGVIYEFPGDCSYMLAGD-CRQR--SFSIL------GDFSHGKRKGVT-VFLGEMFELHLSVDGTLSQGAERLPLPYASSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKMSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHCSAGKECV--CQAMLEYSRACASRGITLSNWHTQSQCSPKCPVGMEYSDCTPTCSTSC----QNVNIQEVCKEECVDGCICPAGKVLDGDRCVDVSQCSCTH--AGRRYPPASYISQDCNTCVCRHGSWECTNEGCPGECLVTGQSHYKT 377 HSP 4 Score: 110.153 bits (274), Expect = 1.988e-23 Identity = 116/470 (24.68%), Postives = 191/470 (40.64%), Query Frame = 2
Query: 191 CSPDCVNG-VCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPF-NSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGM-ELECESQCLPGCYCPRNRYW-DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENE 1585
C +CV+G +C G+ G+RC D + C++ G+ P +Y +CNT + + + +N G EC V H KTFD F++ Y L DC + F + DQ SI+++ + V + P N I ++V S ++++ DL + +D R R + P A ++CGLCG +NG DD+ +++ V+ F NSW + +D + + + PC+ N + + A C ++ C V + Y+ C+ ++C C Q C Y+ AC +G+ V+WRS + C + Q + CGS C + C L + + C+ C GC+CPR + D CVP + C C NGE ++ + + C+C G C NE
Sbjct: 300 CKEECVDGCICPAGKV-----LDGDRCVD-VSQCSCTHAGRRYPPA------SYISQDCNTCVCRHGSWECTNEGCPG-----------ECLVTGQSHYKTFDNKFFTFSGICHYLLAKDCQSNS--FSAVIETAQCADDQDAICTRSITLRFKDLANQTVHLKHGGVVSVDGMDVQTPLINGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYA-GQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSW--KMNADCDDVQMQNSDPCSL----NPKRVRFAEEACAVLLSMKF-EPCHYMVNPEPYVKNCRYDVCACADGQ----------ECLCDAVSSYAVACAARGVLVNWRSHGYC--ELTCPDAQVYEMCGSVCNQTCRSLSLPDTGCDGVCEEGCFCPRGLFLSDAGECVPPQHCSC-HHNGE-IFEPNDMIGDHHSICVC-ESGSMHCTSNE 721
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Match: scospondin (subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]) HSP 1 Score: 501.901 bits (1291), Expect = 6.860e-145 Identity = 357/1263 (28.27%), Postives = 558/1263 (44.18%), Query Frame = 2
Query: 368 TLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCG--QLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFD-GHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWN-GGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQES-KCKEE-----CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLP--GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILF----TMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC 4102
TLL S V G + V + S+ C G H +TFD F + Y L G + VY + + T C +++ +G + + N P G+ V +G F+ ++ + + FD + IY+ GLCG +N DD+ + + +F NSW +D V + + ++ L N+ ++ ++K C +V Y+D C C L+ + ++ + C Y C + I + WR C C GQ FS+C S+C C + +C +C+ GC CP+ Y CV ++ CPC +Y G ++ C TC+C G+W+C E+CE +C + G H TFD++ + C +TAV D L SI I C E GC R + + + + + + VN + LP TG+ +L+ + S+ +L +++ G +++W+ G + I L + KV G+CG + ++ DD T +G E F + +C + + C A +VC ++ S F+ CH + + F C +VC+CG + + C C ++YA CA E LSWR + CP +C + Y +CG C ++C L + C+ + C+ GC CP+G D A +CV + C C+ + + CN C C G W CT C CP S+ F C +TC++ + P C P GC CPE TVL +C+KP ECPC+ N ++Y NDTI + +C N C+C++ +W C Q CSG C G+ HY +FDG+++ F G C YV+ ++ G F + +EN+ CGS+ +CTKS+ N +RG V+ P P ++ + + LF + G+ + WD M + + + +V GLCGNFD +ND R G +E F +SWK+ SCP Q C P R WAK KC ++ + F CH V + YY CV DAC CD G DC C CTAV Y CN + ++WR+ E CP C V++ CG C +C +++D+ C+EGCFCP G + ++ C+ C C + G V + C+ C+C G ++C
Sbjct: 170 TLLPDVGSSPVVRGGLLRGVRDPQASS--TCMSWSGTHFRTFDRKHFHFQGSCTYLLASSTDGT---WAVYISTVCE-----TSGRCRKDLRMMLGLGLVSIHKDNMTVNGLAVPQGEPLFQNGVSVHWLGDFVFVESGLGVRLKFDMENTIYLTVTAEHLTNTRGLCGVYNNNPDDDFTTSTGSVSQYAASFGNSWRVQDQQQSQVCSDAAELG-HSCDLSNDVSLRRDAESACHLLKETPFSHCHHQVDPGPYIDTCLYLFCS-LEVKDRDTAV-------CDTLASYVRECAQQHIIISWRR-RGFCGTRVCPRGQVFSDCVSSCPATCASPRPPAAGQCRDECVGGCECPQGVYLHAGQCVRRDDCPCFHR--RHSYSAGQTIRQRCNTCVC-RAGQWEC-STEKCEAQCSVLGAMHITTFDQKRYGLQTADCRFTAVEDFVDKKL---------SIIIGAGPCA-GGEAGCLREMSITA----LHTTLTVTDAGSVLVNGHRNPLPVV-TGD--LLVRRASSSVLLIQA-FGAQLMWHLDGPLLFITLQPGFAHKVRGLCGTLSWSQHDDFTTPEGDVENSAASFAAKFIVG-SCPAPSAMT--SDPCGTLTQRRQLAERVCSVIH-SNVFQACHDVVDREPFMRLCVSEVCSCGPQAS-----CHCSVLTAYATHCAQEGALLSWRNHTFCPVQCSGGQV-YQECGRLCGTSCADLWDGWSCEPDEGSRVCVSGCQCPDGLAQD-AQGQCVPVHMCPCKSGDTLHPAGSSINNNCNTCVCARGVWNCTDLSCPELNQCPGSLI-FSPRSCLRTCSSPNS--PLDACTEPIHGCICPEGTVLLGDQCVKPDECPCHHNGRLYFTNDTISK---DC-NTCVCREQRWHCGQ-ALCSGTCVATGDPHYITFDGRHYSFLGDCEYVLVQE----SSGLFSVSAENVPCGSTGVTCTKSVTLTIGNTAIHLLRGKAVTVN---GIPVTLPKSYSGSGLILERVGLFVSLSSRLGVTLLWDGGMRVYVRLMPHLHGRVGGLCGNFDGDAENDFTTRQGIMESTPELFGNSWKISPSCPDVADQDLRDPCFINPHRVTWAKKKCAVINQEIFSSCHSEVPYQQYYDWCVFDACGCDSGGDCECLCTAVAAYAEECNRRGVYVRWRSQELCPLQCENGLVYEACGAACSPACPST-PSLSDSLCSALSCVEGCFCPQGTVYHDDACISPSQCPCE--WDGSVFPAGAMVTQHCQNCSCSAGLWQC 1361 HSP 2 Score: 52.373 bits (124), Expect = 8.314e-6 Identity = 36/108 (33.33%), Postives = 49/108 (45.37%), Query Frame = 2
Query: 2489 GSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYE-IRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFD---CGICQKTC 2797
G C C + + +C EC +GC+C G F K CV + C C G +P + + +D CNNCTC NG C G+E CP W + C +TC
Sbjct: 2706 GGACPRVCLDAMADVQCASECYDGCYCSPG--FYLFNKSCVHLQFCPCVHQGALLMPGDSVPRDSCNNCTCVNGEMQC------GEEPCPVHCGWSEWTSWSACSRTC 2805
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Match: CABZ01080550.1 (pep chromosome:GRCz11:25:8269956:8292650:1 gene:ENSDARG00000100621.2 transcript:ENSDART00000166938.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01080550.1) HSP 1 Score: 456.062 bits (1172), Expect = 2.517e-133 Identity = 339/1191 (28.46%), Postives = 529/1191 (44.42%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW--IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEE-FKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCPNE---NMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGI---------CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGL 3964
CS+ H KTFDG+V+ +P Y L DC F +Y + K ++++ I + V K +P + GI+V + ++ + +++++D + V + CGLCG FNGI +N+ IQ + + R P N + E N + DQ +K C D++++ +C + + Y+ C +++C QPE+ +T + Q S+A G WR+A C QC SE GS C C CE + GC+CP +D T C+P EKC C D Y G L+ + E C C G W C+ G C + H+ TFD + F F G C Y ++ + I P C + C ++I V++ +++ + + + + ++ N ++SLPY T + V IL+ V++ ++ I + + ++ K G+CGNFN DD T +GV E F +WK C DR C+ S +A C K+ + E F +CH+ N D ++ +C + CTC + C C FSSYAR CA + + L +C K CP + + C C S Q ++GC CP+G DE CV + C C +G+ P + R +C C NG + C S + CP +F+C C KTC++ ++ C CQ GC CP + G C++P+ CPC + + Y+ IV+ +C IC+ G W+C Q K+C GIC +G HY++FD + F F+G CSY+ +++ CGNK G F +++EN+ CG++ ++C+K++ + G+ T GP Y +V + ++ G+ V WD+ ++ I++ ++ + VCGLCGNF+ +D + +I+ F DSWKV S+CP Q CS+ P+RE WAK +C I+K +FK CH V+ YY+ CV D+C CD G DC CFCTAV Y CNE + + WRT E CP C D + G+ + C C + C PDG+
Sbjct: 4 CSMWGNFHFKTFDGDVYQFPGMCEYNLVSDCQSLIRQFSIYVKRTERSTGPKIS-------RVSITINDIAIELTENQVNVNEAKVTLPVHVSGILVEENTIYTRLYSKMGITVMWNKDDAVMVELDSKYSNRTCGLCGDFNGIPV-----YNEFIQSGRTVGYTEFGNMHRVP---NPTHQCEDPFENVDEQNVVDQCEKYRADCADLLEDEKWSSCSWVLDPEAYIKACTNDLC---NRQPEDEDTTALCATLTEYSRQCSHA---GGNPPAWRTAK-FCNV-QCPYNMVHSESGSPCMDTCSHKDTNALCEEHNIDGCFCPPGTVFDDISDTGCIPAEKCQCKHDR---VYSSGEVLRKSEEECYC-QEGSWVCMSIPG-PGLCAVEEGSHFTTFDGKEFTFHGDCNYLLSKDCEESKFIILGQIVP---------CFTHETDTCLKSI-VVLFDNDKKNPLFIKADGTVQHN-AEVSLPYM-TADFTVFRPSSFHIILQTVFGLQVQVQLVPLMQVYITVDQSFQGKTCGLCGNFNKVLADDLKTPQGVVEGTAVSFANAWKAQSNC-PDRS-ERMDDPCSYSSDSEHFAEHWCSKMKDKESLFAQCHATVNPDSYYKRCKYSSCTCEKSED-----CLCTVFSSYARACAAKGVILEGWREVVCDKYIKDCPASQSYSYELQSCQRTCVSLASERQSCNVDFVPVDGCACPDGLYQDEN-NLCVPMEKCPCYHNGQKIHPGKSLTIRDEHCVCMNGKFHCRSWKTQILG-CPSPKVFFNCSTAGPEEYGLECAKTCSHQEVDCFSLDCQ-SGCQCPFGLLDDGRGHCVQPYNCPCKHDGQYYAPGSVIVK---DCNKCSICRWGNWLCSQ-KKCPGICTIYGSGHYKTFDQQRFGFRGDCSYIAAQNKCGNKTGNFHVVTENMPCGTTGTTCSKAVGILLGRTWLLLSDGTVTATDVGT-----GPNIKYNERNVGMYLVIDAAIGLTVLWDRKTTVRIILQPQHMDDVCGLCGNFNGNGKDDFTTQGNLPTTNIMEFVDSWKVLSTCPDAQPDFDPCSQTPNRETWAKIQCSIIKEVTFKDCHSKVDPNPYYENCVKDSCSCDTGGDCECFCTAVAAYAQACNEAGVCVVWRTPEICPVYC--------------DYYNEPGECTWHYSPCHTPCYKTCLNPDGI 1121 HSP 2 Score: 171.785 bits (434), Expect = 2.620e-42 Identity = 137/488 (28.07%), Postives = 231/488 (47.34%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTV---LHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIAC-GXXXXXCTKSIKFIYLN--RVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWD--KAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP-VPQKPPFMCSKRPDREPWAKAKCEILKSDS--FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTN------ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW-QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEI 4150
CP +M + G C TC++ D + + GCFCP TV + + CI +C C ++++YS + + + + C C++G WVC + G+C +H+ +FDGK F F G C+Y++S+D +K F ++ + + C + +C KSI ++ N + F++ + P F H IL T+ G+QV+ M + I + + +Q K CGLCGNF+ + +D++ G +E V+FA++WK S+CP ++ CS D E +A+ C +K F +CH VN + YYKRC +C C++ +DC+C T Y C + L+ WR ++CP + + ++C +C+ + + V ++GC CPDGL+ +NN CV + C C K + G + E C C G F C + + + L P++
Sbjct: 267 CPYNMVHSESGSPCMDTCSHKDTNALCEEHNIDGCFCPPGTVFDDISDTGCIPAEKCQC-KHDRVYSSGEVLRKS----EEECYCQEGSWVC-MSIPGPGLCAVEEGSHFTTFDGKEFTFHGDCNYLLSKDCEESK---FIILGQIVPCFTHETDTCLKSIVVLFDNDKKNPLFIKADGTVQHNAEVSLPYMTADFTVFRPSSFHIILQTVFGLQVQVQLVPLMQVYITVDQSFQGKTCGLCGNFNKVLADDLKTPQGVVEGTAVSFANAWKAQSNCPDRSERMDDPCSYSSDSEHFAEHWCSKMKDKESLFAQCHATVNPDSYYKRCKYSSCTCEKSEDCLC--TVFSSYARACAAKGVILEGWREVVCDKYIKDCPASQSYSYELQSCQR----TCVSLASE-RQSCNVDFVPVDGCACPDGLYQDENNLCVPMEKCPCYH--NGQKIHPGKSLTIRDEHCVCMNGKFHC-RSWKTQILGCPSPKV 735 HSP 3 Score: 95.1301 bits (235), Expect = 7.331e-19 Identity = 97/384 (25.26%), Postives = 163/384 (42.45%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENI--ACGXXXXXCTKSIKFIYLN-----------RVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQ---KPPFMCSKRP---DREPWAKAKC-EILKSDSFKKCHDVVNVEHYYKRCVADACECDRGD-DCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW---QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
IC WG H+++FDG ++F G C Y + D C + + F + + + G S + +I I + +V V S +V NT Y L++ GI V W+K ++ + + KY N+ CGLCG+F+ + +SG V + + +H P P + PF D+ +A C ++L+ + + C V++ E Y K C D C D D C + +Y +C+ WRT + C C V G+ C D+C + N C++ I+GCFCP G + + C+ + C+C + Y++G+ + + EEC C EG++ C +
Sbjct: 3 ICSMWGNFHFKTFDGDVYQFPGMCEYNLVSD-CQSLIRQFSIYVKRTERSTGPKISRVSITINDIAIELTENQVNVNEAKVTLPVHVSGILVEENTIYTR-----------------LYSKMGITVMWNKDDAVMVELDSKYSNRTCGLCGDFNGIPVYNEFIQSGRT----VGYTEFGNMHRV-PNPTHQCEDPFENVDEQNVVDQCEKYRADCADLLEDEKWSSCSWVLDPEAYIKACTNDLCNRQPEDEDTTALCATLTEYSRQCSHAGGNPPAWRTAKFCNVQCPYNMVHSESGSPCMDTC----SHKDTNALCEEHNIDGCFCPPGTVFDDISDTGCIPAEKCQCKH---DRVYSSGEVLRKSEEECYCQEGSWVCMSI 356
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Match: otog (otogelin [Source:ZFIN;Acc:ZDB-GENE-120228-1]) HSP 1 Score: 437.958 bits (1125), Expect = 2.203e-124 Identity = 334/1214 (27.51%), Postives = 536/1214 (44.15%), Query Frame = 2
Query: 281 CVGGCSNGGKCVAPNQCLC-LDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALV-KNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKV-NFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTN-ELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG------------ICQKTCTN--LDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRG----SNP--IVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS--SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC 3832
C C NGG+CV P+ C C L +G C T V N + C ++ +TFDG + +P + YTL DC A V+ D + C+ S+ + + + I F++ K +P + + + + +I + + + ++Y+ CGLCG FN DD + V +++ F NSW + +P YP L + K +C +++ ++C V Y+ C +++C + P + + C++ +Y+ AC + DWR+ +C C + F EC + C +C M ++ + QCL GCYCP + ++ +CV CPC ++ Y G+ ++ +C C C G W C E+ C G C ++GD + +FD R + F C+Y VL + ++TIQN C N + C +++ +++ ED + I + S E+ + + +ISLPY + I ++S+ +++K G+ + +N R+ ++L +++R+ G+CG FNGN DD + G+ E F +W+ + C R + C + + YA + C L + E F CH + F QC DVC CG AL C C + YAR C + + +R++ C CP+ M+Y C S C C L C +EC EGC CPEG F+ CV ++C C F G Y P ++ C +G + ++ E CP + DCG C++TC L+L C + +PGC CP + H EC KP CPC K Y D + + C C+C+ G + CE + C +C +G+ HY +FDG F++ G C + + L + +EN+ C S C KS+ I + R F NP ++ R A+ W + T + E + V WD+ ++ I ++Q ++ GLCGNFD + N+MR F +SW + P + P C+ P REP+AK +C IL S+ F+ CH VV+V +Y C+ D C C+RG DC CFCT+V Y RC + I + WR+ CP+ C
Sbjct: 91 CASPCLNGGRCVHPDSCNCTLYQAAGTRCQT------------------VPNLGFEREMTCRSWGQYNYETFDGLYYYFPGKCSYTLLRDCEDNA-RSSVHIQVHNDPECSSSPYSCTRSISLFLPWEGEIRL--QHFSVTFKGQSVLLPHHIHDVELERISDYIVVSQPHVFTLAWQGLTSSVYIKMSPEFVGHTCGLCGNFNADTQDDLKTSYGVFTQDLAMFGNSWAEEEPELARCTVVPSLYPSPCATL-DPHVLLKVEEVCSTLLREPF-QSCHEFVSPYSYMASCSNDLC---LSGPNGAVV-------CQVFTEYARACAHADHPLHDWRTHFPVCNV-DCPDELLFRECITCCPMHCKIEQMCIDSKLQCLDGCYCPDDLIFEDGVCVKASDCPC--EHHGMLYPSGHIIQEDCNNCTC-FGGIWNCTEHN-CPGECSVTGDMFFQSFDGRVYTFPATCQY---------VLAKSRNSGKFTVTIQNAPCGANLDGACIQSVNLVVNEDP-RTEITMSHSGEVFLASQFRISLPY---SDDMFQIQELSSMFVQVKLSQGLWLQYNWKEFRLYLQLEEQWRDNTLGLCGTFNGNIQDDFLAPSGMIESTPFLFGNAWRVSSAC-VPRPSLPQLDPCDTHQQAASYAVEKCDVLTQ-EVFSACHEYVSPVGFQLQCRADVCKCG-----AL--CLCSILAHYARTCRRHGVIIEFRSHLPECAVSCPS-TMEYGVCVSACQRRCLFLSLPQPCGDECEEGCVCPEGTYFNMHTHTCVPRNECPCSFLGADYSPGDVIITSSGVQICQDGKLVSQTSIIE--TLCPAGQFYEDCGNRVDGPSASKGLACEQTCETYLLNLTCSTHEPCVPGCVCPAGLLKHGDECFKPRACPCLWKGKEYYPGDKVS---SPCHQ-CVCQHGTFQCE-FRPCPSMCTAYGDRHYRTFDGFLFDYIGVCKVYLVKSSLDAML---TVTAENVDCFESGVICRKSLH-ISVGRSFIVFDDDTGRPNPSSVIDRQR--------AYIWQAGYYT-IVHLLGEDLTVLWDRKTTIHIQAGPRWQGRLTGLCGNFDQKTANEMRTPENIDSSTPQEFGNSWTAAECVNSPDIRHP---CNLNPLREPFAKRQCGILLSEVFQVCHPVVDVTWFYMNCLVDTCGCNRGGDCECFCTSVAAYAHRCCQKGIPIDWRSPSICPYDC 1220 HSP 2 Score: 161.77 bits (408), Expect = 4.447e-39 Identity = 115/462 (24.89%), Postives = 212/462 (45.89%), Query Frame = 2
Query: 2747 CPKSMAWFDCGICQKTCTNLDLVCPKGQCQ-LPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXX-XCTKSIKFIY-LNRVFQFVRGSNPIVSRNTNYPPGGPTA--FYWTSDVDTHFILFTM-EGIQVKWD-KAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPF-MCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTN-ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW--QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAF 4096
CP + + +C C ++ +C + Q L GC+CP++ + +G C+K +CPC + +Y I +C N C C G W C ++ C G C G+ ++SFDG+ + F +C YV+++ G F + +N CG++ +C +S+ + + + + V + + P + + ++ + F+ + +G+ ++++ K L + + E++++ GLCG F+ I +D A SG IE F ++W+V S+C P P P C +A KC++L + F CH+ V+ + +C AD C+C +C C+ + Y C + + +++R++ C +C + C + C C+ + C C EGC CP+G ++ + CV R C C Y+ GD +I C +G
Sbjct: 404 CPDELLFRECITCCPMHCKIEQMCIDSKLQCLDGCYCPDDLIFEDGVCVKASDCPCEHHGMLYPSGHIIQE---DCNN-CTCFGGIWNCTEHN-CPGECSVTGDMFFQSFDGRVYTFPATCQYVLAK---SRNSGKFTVTIQNAPCGANLDGACIQSVNLVVNEDPRTEITMSHSGEVFLASQFRISLPYSDDMFQIQELSSMFVQVKLSQGLWLQYNWKEFRLYLQLEEQWRDNTLGLCGTFNGNIQDDFLAPSGMIESTPFLFGNAWRVSSACVPRPSLPQLDPCDTHQQAASYAVEKCDVLTQEVFSACHEYVSPVGFQLQCRADVCKCG----ALCLCSILAHYARTCRRHGVIIEFRSHLPECAVSCPSTMEYGVCVSACQRRCLFLSLP----QPCGDECEEGCVCPEGTYFNMHTHTCVPRNECPCS--FLGADYSPGDVIITSSGVQICQDGKL 847 HSP 3 Score: 119.783 bits (299), Expect = 2.859e-26 Identity = 94/374 (25.13%), Postives = 160/374 (42.78%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKL-ISENIACGXXXXXCTKSIKF-------IYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMS-LEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSW-----KVHSSCPVPQKPPFMCSKR-PDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTN-ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTK 4108
C++WG+ +YE+FDG Y+ F G CSY + D N + + + + C SS SCT+SI I L +G + ++ + + + + ++ + W S + I + ++ CGLCGNF+ +D++ G +D+ F +SW ++ VP P C+ P + C L + F+ CH+ V+ Y C D C G + C +Y C + L WRT+ C C + +++ C CP C I + D+ K C++GC+CPD L +++ CV+ C C + Y +G + +C C C+ G + CT+
Sbjct: 133 CRSWGQYNYETFDGLYYYFPGKCSYTLLRDCEDNARSSVHIQVHNDPECSSSPYSCTRSISLFLPWEGEIRLQHFSVTFKGQSVLLPHHIHD--------VELERISDYIVVSQPHVFTLAWQGLTSSVYIKMSPEFVGHTCGLCGNFNADTQDDLKTSYGVFTQDLAMFGNSWAEEEPELARCTVVPSLYPSPCATLDPHVLLKVEEVCSTLLREPFQSCHEFVSPYSYMASCSNDLCL--SGPNGAVVCQVFTEYARACAHADHPLHDWRTHFPVCNVDCPDELLFRECITCCPMHC-KIEQMCIDS---KLQCLDGCYCPDDLIFEDGVCVKASDCPCEH--HGMLYPSGHIIQEDCNNCTCFGGIWNCTE 490 HSP 4 Score: 72.7886 bits (177), Expect = 4.605e-12 Identity = 90/374 (24.06%), Postives = 142/374 (37.97%), Query Frame = 2
Query: 1568 QCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTI---GVIIGEDEDQVAINLL------SSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILW---NGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGV---DEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTS-TCRLLQESKCKEECI---EGCFCPEGETFDEA-AKECVKISDCKC 2629
+C +C RC + D + ITFD + Y L N T+ SI +Q + D++ + + I ++V +N L +S K F K TGN + ++S G++I W G IE+ S R G+CG+ +GN ++D G +++D F+ SW+ T + C S C + + F CH+ F + DV + C A ++YA C NI + WR+++ CP CP N++Y C CT+ +C + E+C EGC CPEG + C+ S C C
Sbjct: 2298 KCCPRWDCPCRCSVFPDLNLITFDGKSVAIYKAALYVVTQ-------LPNETV---SILVQECSGPDDTLVWNFTNLCLAALNITHKSNEVLVNRLQRRLYVNSRYAKPRFKKYGFEILDTGN-----------MYLIRSPAGLKIQWFHTTGMMVIEMD-SYNNRLPTMGLCGSCDGNPLNDLTLSNGTVVPEQEDPAVFIDSWQIPNTTSYVSNSRRREVNCTTSDCSQ------CFNMLNNNTFTLCHAYVPPGVFCEMWVRDV---------EYVNNPCIALAAYAASCHKFNICMEWRSSDFCPFVCPG-NLRYQACLPACTAPSCPNQEFEFVPEQCTGLSEGCVCPEGTLLHRPYSALCIPPSKCAC 2633 HSP 5 Score: 67.3958 bits (163), Expect = 2.253e-10 Identity = 58/245 (23.67%), Postives = 106/245 (43.27%), Query Frame = 2
Query: 3326 DVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKV--CGLCGNFDLRIDNDMRARSGEI---EKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCE----ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNIC-PDSCIDIGKNVTDN--TGCKKVCIEGCFCPDG--LHWQNNE-CVERKACECVD 4015
D +++ + G++++W + ++ + Y N++ GLCG+ D ND+ +G + ++D F DSW++ P ++ + R + C +L +++F CH V + + V D + C A+ Y A C+++ I ++WR+++ CP C G ++ C C SC + TG EGC CP+G LH + C+ C C D
Sbjct: 2407 DTGNMYLIRSPAGLKIQWFHTTGMMVIEMDSYNNRLPTMGLCGSCDGNPLNDLTLSNGTVVPEQEDPAVFIDSWQI------PNTTSYVSNSRRREVNCTTSDCSQCFNMLNNNTFTLCHAYVPPGVFCEMWVRDVEYVNNP------CIALAAYAASCHKFNICMEWRSSDFCPFVCPGNLRYQACLPACTAPSCPNQEFEFVPEQCTGLS----EGCVCPEGTLLHRPYSALCIPPSKCACTD 2635
BLAST of von Willebrand factor vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 566.614 bits (1459), Expect = 1.328e-166 Identity = 385/1262 (30.51%), Postives = 595/1262 (47.15%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD-LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP-CNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV--------WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNT---GDKVI------RECEECNCYEGAFRC 4102
+S CS+ +HI+TFDG + + Y L DC + F+ D + K + S+ + +G I + A +P+ S GI + S + K+ + + D N+ V P K CGLCG +N DD+ ++ +N F NSW R R YP N + + + + CQ + ++ C V D Y+ +C+D++CEC + NC C+ +Y+ C G+ TICK P C G E++EC S C K C L + C+ QC+ GC CP + DG C+P +C C+ Y G ++ +C +C C +G W+C NE+C G CF++G H+ +FD +HF F G C+Y +L +++ + S+TI+ C D+ + C R+ + + D + I L + ++ I LP G ++ +S +RL + +++ W+G ++ IKLS Y ++G+CGN+NGN+ DD +T G+ E ++ F SWK N C + + C L+ + ++ +VC L +S F+ CH+ N + C +DVC+C NG C C A S+YA C+ + I + WRT E CP +C +E Y QCGS C TCR L + C+E C+EGC+CP G +E ECV SDC C + G + P ++ + + C C NG C+++D K CP C KTC N +L C C + GC CP+ V H +C+ P CPC+ K Y+ ++ +C N C+C++ KW C +N C C G +HY +FDG ++F G C YV+++D+CG GTF+++ N CG S CTK I ++ N + I P T+F +FIL + V WDK M + + + E Y++KVCGLCGNFD +ND+ + + +IE D VNF +SWKV+ C P +C ++ A+ C IL D F +C VVN E Y++ C D C C+ DC CFC ++ Y C + I + WR++ CP +C K W+ +C CP +C + C C+EGC +CP G L + C+E K C P+ ++ +T G K+I + C+ C+C +C
Sbjct: 24 WSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCNKHS------FSLLADYRNGKKK-----SVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHG----GARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEEL-------NCP---CQTFLEYARTCAQHGVITSNWPTHTICK-PLCPFGMEYNECVSPCVKTCQSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKR--YPPGSAIRRDCNSCTC-QNGAWEC-SNEDCPGECFVTGQSHFKSFDNKHFTFSGICQY---------LLATDAMDSTFSVTIETVQCADDIDAVCTRSASLRL-LDMHNITIKLKHGGGVSLDGQDILLPLVQ-GFLRIQTTVLSA--VRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTD--LMKQDTDPCNLNPKRARFSEEVCFALMDSA-FQSCHNEVNPAPYLKNCRYDVCSCS---NGK--ECLCSALSTYAAACSRKGILIDWRTPEFCPIQC-SEGKIYQQCGSPCNQTCRSLSHPDVDCREFCMEGCYCPPGLYTNEDG-ECVPKSDCSCYYDGELFQPDDVFSNHYSMCYCENGLMHCSTSDVPSVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGC-ISGCTCPDGMVRHKSKCMFPQRCPCFHAGKEYAPGSSV---EIDC-NTCVCENRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGTFRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHI-----KKPLSDETSFDVLKS-GNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSPLSLCKDNLMKQMAAENACNILMGDLFAECAKVVNPEPYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTC-----QHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDC----PVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC 1211 HSP 2 Score: 199.134 bits (505), Expect = 2.987e-50 Identity = 148/490 (30.20%), Postives = 222/490 (45.31%), Query Frame = 2
Query: 1340 GSNCGKNCGQLGMELECESQ-CLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK---------YNQCGSRCTSTCRLLQESKCKEECIEGC--FCPEGETFDEAAKECVKISDCK-CEF------HGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
G C K C ELEC S C+ GC CP + C+ ++CPC E Y G ++++C TC+C + KW+C EN C+ C G HY+TFD + F G C+Y V I + N C + E C + I ++ E + L++ +I + K L D+ S D+L+ L + + W+ G R+ + L + YR+KV G+CGNF+G E +D ++ E D F SWK NP C +D G V C + A C+ L + F +C N + +++ CT+D CTC + A C C++ ++YA CA IN+ WR++ LCP+ C +N + YN C + C TC+ + C +C+EGC +CP G+ DE ++ C++ DC C HGR + RC C C N C N CE
Sbjct: 765 GIECAKTCQNY--ELECVSHGCISGCTCPDGMVRHKSKCMFPQRCPCFHAGKE--YAPGSSVEIDCNTCVCE-NRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGV------TGTFRILVGNEGCGFSGEK-CTKRITILFRNGEIE-----LTNEQIHIK--------------KPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLC-ADFGAVSPLSLCKDNLMKQMAAENACNILM-GDLFAECAKVVNPEPYWEICTYDTCTCESAGDCA---CFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC--NACE 1215 HSP 3 Score: 141.354 bits (355), Expect = 1.164e-32 Identity = 97/365 (26.58%), Postives = 174/365 (47.67%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPD-REPWAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYE-IGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +GE H ++FDG +++F G CSY+++ D C +F L+++ + K +YL F F+ G+ ++ + P + S+ + + G VK D + ++++++P K+ NK CGLCGN++ ++D + G + ++ +FA+SW +H C P C+ + E +C++LK S +F +CH +V+ + Y C D CEC ++ C C +Y C ++ I W T+ C C + C + C +C +++ N C++ CI+GC CPDG + C+ C C+ + +Y G + R+C C C GA+ C+
Sbjct: 30 CSLFGENHIQTFDGTFYDFYGDCSYMLAGD-CNKH--SFSLLAD-------YRNGKKKSVSLYLGEYFDIHLFLDGTATQGEKHISMPYAS-NGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTC----QSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIH--SGKRYPPGSAIRRDCNSCTCQNGAWECS 375 HSP 4 Score: 100.138 bits (248), Expect = 3.458e-20 Identity = 111/432 (25.69%), Postives = 172/432 (39.81%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTA---FYWTSDVD-THFILFTMEGIQVKWDKAMSLEIMI--PEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDR---EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVDPITNLKYNTGDKVIR-------ECEECNCYEG------AFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDG 4249
C +C H +FDG F+ G+CSYV+ D ++ K+I N C ++ +C KS++ + + + SN VS N + P P A F T + H + G + A + ++ P + + GLCG D ND G + D F W V S C +PD+ +P + ++C+I+ S +F+ CH + + Y+ C +C G D C + YV C + + WRT + CP C V+ +C C C GK + + T C EGCFCP+G N +CV K C +C D +Y +++ C C C E A C + C P + C +YE +C C N + C +G
Sbjct: 1951 CPCMCMGSSTRHIVTFDGLDFKLMGACSYVLFHD----EVHDVKVILHNAECNTTNHQTCMKSVEVKH--NKYSILLSSNMQVSVNGDIVPL-PYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLG------RTCETKPDKACTQPIS-SECKIMLSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKEC---GKTL-NGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTD-----RYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTC--DVNPVPHCENG 2351 HSP 5 Score: 92.8189 bits (229), Expect = 5.565e-18 Identity = 115/504 (22.82%), Postives = 194/504 (38.49%), Query Frame = 2
Query: 1523 KLNCETCICGM-HGKWQCIENEE-------CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS-KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH-------KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAA----KECVKISDCKCEFHGRF--YLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGIC---------QKTCTNLDLVCPKGQCQ---LPGCFCPENTVLHN-GECIKPFECPCYM 2917
K+NCE M H ++ EE C C S H +TFD F G C Y +L + + + + + N C + C +++ V ++ +I L S+ ++ VN + LPY + L ++ ++ ++ V + ++L+ + + +G+CG + N ++D G D KF+ W +V+ +L R+ +K C K+ S+ F+ CH+ +F C C H + CE ++Y C + + ++WRT + CP +CP M Y+ C C C + L + C + EGCFCPEG+ K C + +D E H ++P C+ C C I CT+ C ++ CG C ++ C + + VC C +P C VL N G C +EC C +
Sbjct: 1921 KVNCEKIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSY----------VLFHDEVHDVKVILHNAECNTTNHQTCMKSVEV----KHNKYSILLSSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEV-KIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWT----------VVD-----SLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASC-----------HGQDICEVIAAYVHVCRINGVCINWRTPDFCPMECPRL-MVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIP---TNAPCSICMCLENRMINCTAMPC-------PTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTCDVNPVPHCENGLVPVLTNPGHCKPNYECACRI 2372 HSP 6 Score: 56.225 bits (134), Expect = 8.026e-7 Identity = 67/335 (20.00%), Postives = 119/335 (35.52%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD----RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKC 1471
HI TFDG F Y LF D + + + NA + + +T C S+++ +I+L N V + +P+ ++ V+ G + I L +F + + P + GLCG + +D+ + + + F+ W D ++ R E P A Q + C+ M+ C + Y +C++ C C++ Y + C + G+ ++WR+ C +C + C C K CG+ C GC+CP CV ++ C
Sbjct: 1962 HIVTFDGLDFKLMGACSYVLFHDEVHDVKV--ILHNAECNTTNHQT---CMKSVEVKHNKYSILL-SSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVD----SLGRTCETKPDKACT-------QPISSECKIMLSKTF-EACHTIFPPKPYFTVCEEASCH--------------GQDICEVIAAYVHVCRINGVCINWRTP-DFCPM-ECPRLMVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVC 2262
BLAST of von Willebrand factor vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 564.303 bits (1453), Expect = 7.695e-166 Identity = 388/1277 (30.38%), Postives = 599/1277 (46.91%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD-LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP-CNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSM---------AWFDC-----------------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV--------WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNT---GDKVI------RECEECNCYEGAFRC 4102
+S CS+ +HI+TFDG + + Y L DC + F+ D + K + S+ + +G I + A +P+ S GI + S + K+ + + D N+ V P K CGLCG +N DD+ ++ +N F NSW R R YP N + + + + CQ + ++ C V D Y+ +C+D++CEC + NC C+ +Y+ C G+ TICK P C G E++EC S C K C L + C+ QC+ GC CP + DG C+P +C C+ Y G ++ +C +C C +G W+C NE+C G CF++G H+ +FD +HF F G C+Y +L +++ + S+TI+ C D+ + C R+ + + D + I L + ++ I LP G ++ +S +RL + +++ W+G ++ IKLS Y ++G+CGN+NGN+ DD +T G+ E ++ F SWK N C + + C L+ + ++ +VC L +S F+ CH+ N + C +DVC+C NG C C A S+YA C+ + I + WRT E CP +C +E Y QCGS C TCR L + C+E C+EGC+CP G +E ECV SDC C + G + P ++ + + C C NG C+++D F P+S DC GI C KTC N +L C C + GC CP+ V H +C+ P CPC+ K Y+ ++ +C N C+C++ KW C +N C C G +HY +FDG ++F G C YV+++D+CG GTF+++ N CG S CTK I ++ N + TN P + +FIL + V WDK M + + + E Y++KVCGLCGNFD +ND+ + + +IE D VNF +SWKV+ C P +C ++ A+ C IL D F +C VVN E Y++ C D C C+ DC CFC ++ Y C + I + WR++ CP +C K W+ +C CP +C + C C+EGC +CP G L + C+E K C P+ ++ +T G K+I + C+ C+C +C
Sbjct: 24 WSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCNKHS------FSLLADYRNGKKK-----SVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHG----GARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEEL-------NCP---CQTFLEYARTCAQHGVITSNWPTHTICK-PLCPFGMEYNECVSPCVKTCQSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKR--YPPGSAIRRDCNSCTC-QNGAWEC-SNEDCPGECFVTGQSHFKSFDNKHFTFSGICQY---------LLATDAMDSTFSVTIETVQCADDIDAVCTRSASLRL-LDMHNITIKLKHGGGVSLDGQDILLPLVQ-GFLRIQTTVLSA--VRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTD--LMKQDTDPCNLNPKRARFSEEVCFALMDSA-FQSCHNEVNPAPYLKNCRYDVCSCS---NGK--ECLCSALSTYAAACSRKGILIDWRTPEFCPIQC-SEGKIYQQCGSPCNQTCRSLSHPDVDCREFCMEGCYCPPGLYTNEDG-ECVPKSDCSCYYDGELFQPDDVFSNHYSMCYCENGLMHCSTSDVPSGYF-PESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGC-ISGCTCPDGMVRHKSKCMFPQRCPCFHAGKEYAPGSSV---EIDC-NTCVCENRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGTFRILVGNEGCGFSGEKCTKRITILFRNGEIEL-----------TNEQPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSPLSLCKDNLMKQMAAENACNILMGDLFAECAKVVNPEPYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTC-----QHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDC----PVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC 1220 HSP 2 Score: 197.208 bits (500), Expect = 1.105e-49 Identity = 148/490 (30.20%), Postives = 220/490 (44.90%), Query Frame = 2
Query: 1340 GSNCGKNCGQLGMELECESQ-CLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK---------YNQCGSRCTSTCRLLQESKCKEECIEGC--FCPEGETFDEAAKECVKISDCK-CEF------HGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
G C K C ELEC S C+ GC CP + C+ ++CPC E Y G ++++C TC+C + KW+C EN C+ C G HY+TFD + F G C+Y V I + N C + E C + I + L + EI++ N + L D+ S D+L+ L + + W+ G R+ + L + YR+KV G+CGNF+G E +D ++ E D F SWK NP C +D G V C + A C+ L + F +C N + +++ CT+D CTC + A C C++ ++YA CA IN+ WR++ LCP+ C +N + YN C + C TC+ + C +C+EGC +CP G+ DE ++ C++ DC C HGR + RC C C N C N CE
Sbjct: 779 GIECAKTCQNY--ELECVSHGCISGCTCPDGMVRHKSKCMFPQRCPCFHAGKE--YAPGSSVEIDCNTCVCE-NRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGV------TGTFRILVGNEGCGFSGEK-CTKRITI------------LFRNGEIELT------------NEQPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLC-ADFGAVSPLSLCKDNLMKQMAAENACNILM-GDLFAECAKVVNPEPYWEICTYDTCTCESAGDCA---CFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC--NACE 1224 HSP 3 Score: 141.354 bits (355), Expect = 1.168e-32 Identity = 97/365 (26.58%), Postives = 174/365 (47.67%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPD-REPWAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYE-IGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +GE H ++FDG +++F G CSY+++ D C +F L+++ + K +YL F F+ G+ ++ + P + S+ + + G VK D + ++++++P K+ NK CGLCGN++ ++D + G + ++ +FA+SW +H C P C+ + E +C++LK S +F +CH +V+ + Y C D CEC ++ C C +Y C ++ I W T+ C C + C + C +C +++ N C++ CI+GC CPDG + C+ C C+ + +Y G + R+C C C GA+ C+
Sbjct: 30 CSLFGENHIQTFDGTFYDFYGDCSYMLAGD-CNKH--SFSLLAD-------YRNGKKKSVSLYLGEYFDIHLFLDGTATQGEKHISMPYAS-NGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTC----QSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIH--SGKRYPPGSAIRRDCNSCTCQNGAWECS 375 HSP 4 Score: 100.138 bits (248), Expect = 3.977e-20 Identity = 111/432 (25.69%), Postives = 172/432 (39.81%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTA---FYWTSDVD-THFILFTMEGIQVKWDKAMSLEIMI--PEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDR---EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVDPITNLKYNTGDKVIR-------ECEECNCYEG------AFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDG 4249
C +C H +FDG F+ G+CSYV+ D ++ K+I N C ++ +C KS++ + + + SN VS N + P P A F T + H + G + A + ++ P + + GLCG D ND G + D F W V S C +PD+ +P + ++C+I+ S +F+ CH + + Y+ C +C G D C + YV C + + WRT + CP C V+ +C C C GK + + T C EGCFCP+G N +CV K C +C D +Y +++ C C C E A C + C P + C +YE +C C N + C +G
Sbjct: 1921 CPCMCMGSSTRHIVTFDGLDFKLMGACSYVLFHD----EVHDVKVILHNAECNTTNHQTCMKSVEVKH--NKYSILLSSNMQVSVNGDIVPL-PYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLG------RTCETKPDKACTQPIS-SECKIMLSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKEC---GKTL-NGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTD-----RYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTC--DVNPVPHCENG 2321 HSP 5 Score: 92.8189 bits (229), Expect = 5.320e-18 Identity = 115/504 (22.82%), Postives = 194/504 (38.49%), Query Frame = 2
Query: 1523 KLNCETCICGM-HGKWQCIENEE-------CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS-KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH-------KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAA----KECVKISDCKCEFHGRF--YLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGIC---------QKTCTNLDLVCPKGQCQ---LPGCFCPENTVLHN-GECIKPFECPCYM 2917
K+NCE M H ++ EE C C S H +TFD F G C Y +L + + + + + N C + C +++ V ++ +I L S+ ++ VN + LPY + L ++ ++ ++ V + ++L+ + + +G+CG + N ++D G D KF+ W +V+ +L R+ +K C K+ S+ F+ CH+ +F C C H + CE ++Y C + + ++WRT + CP +CP M Y+ C C C + L + C + EGCFCPEG+ K C + +D E H ++P C+ C C I CT+ C ++ CG C ++ C + + VC C +P C VL N G C +EC C +
Sbjct: 1891 KVNCEKIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSY----------VLFHDEVHDVKVILHNAECNTTNHQTCMKSVEV----KHNKYSILLSSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEV-KIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWT----------VVD-----SLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASC-----------HGQDICEVIAAYVHVCRINGVCINWRTPDFCPMECPRL-MVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIP---TNAPCSICMCLENRMINCTAMPC-------PTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTCDVNPVPHCENGLVPVLTNPGHCKPNYECACRI 2342 HSP 6 Score: 55.8398 bits (133), Expect = 8.644e-7 Identity = 67/335 (20.00%), Postives = 119/335 (35.52%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD----RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKC 1471
HI TFDG F Y LF D + + + NA + + +T C S+++ +I+L N V + +P+ ++ V+ G + I L +F + + P + GLCG + +D+ + + + F+ W D ++ R E P A Q + C+ M+ C + Y +C++ C C++ Y + C + G+ ++WR+ C +C + C C K CG+ C GC+CP CV ++ C
Sbjct: 1932 HIVTFDGLDFKLMGACSYVLFHDEVHDVKV--ILHNAECNTTNHQT---CMKSVEVKHNKYSILL-SSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVD----SLGRTCETKPDKACT-------QPISSECKIMLSKTF-EACHTIFPPKPYFTVCEEASCH--------------GQDICEVIAAYVHVCRINGVCINWRTP-DFCPM-ECPRLMVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVC 2232
BLAST of von Willebrand factor vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 563.148 bits (1450), Expect = 1.830e-165 Identity = 390/1280 (30.47%), Postives = 601/1280 (46.95%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD-LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP-CNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSM---------AWFDC-----------------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQK-----PPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV--------WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNT---GDKVI------RECEECNCYEGAFRC 4102
+S CS+ +HI+TFDG + + Y L DC + F+ D + K + S+ + +G I + A +P+ S GI + S + K+ + + D N+ V P K CGLCG +N DD+ ++ +N F NSW R R YP N + + + + CQ + ++ C V D Y+ +C+D++CEC + NC C+ +Y+ C G+ TICK P C G E++EC S C K C L + C+ QC+ GC CP + DG C+P +C C+ Y G ++ +C +C C +G W+C NE+C G CF++G H+ +FD +HF F G C+Y +L +++ + S+TI+ C D+ + C R+ + + D + I L + ++ I LP G ++ +S +RL + +++ W+G ++ IKLS Y ++G+CGN+NGN+ DD +T G+ E ++ F SWK N C + + C L+ + ++ +VC L +S F+ CH+ N + C +DVC+C NG C C A S+YA C+ + I + WRT E CP +C +E Y QCGS C TCR L + C+E C+EGC+CP G +E ECV SDC C + G + P ++ + + C C NG C+++D F P+S DC GI C KTC N +L C C + GC CP+ V H +C+ P CPC+ K Y+ ++ +C N C+C++ KW C +N C C G +HY +FDG ++F G C YV+++D+CG GTF+++ N CG S CTK I ++ N + I P T+F +FIL + V WDK M + + + E Y++KVCGLCGNFD +ND+ + + +IE D VNF +SWKV+ C K P +C ++ A+ C IL D F +C VVN E Y++ C D C C+ DC CFC ++ Y C + I + WR++ CP +C K W+ +C CP +C + C C+EGC +CP G L + C+E K C P+ ++ +T G K+I + C+ C+C +C
Sbjct: 35 WSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCNKHS------FSLLADYRNGKKK-----SVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHG----GARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEEL-------NCP---CQTFLEYARTCAQHGVITSNWPTHTICK-PLCPFGMEYNECVSPCVKTCQSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKR--YPPGSAIRRDCNSCTC-QNGAWEC-SNEDCPGECFVTGQSHFKSFDNKHFTFSGICQY---------LLATDAMDSTFSVTIETVQCADDIDAVCTRSASLRL-LDMHNITIKLKHGGGVSLDGQDILLPLVQ-GFLRIQTTVLSA--VRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTD--LMKQDTDPCNLNPKRARFSEEVCFALMDSA-FQSCHNEVNPAPYLKNCRYDVCSCS---NGK--ECLCSALSTYAAACSRKGILIDWRTPEFCPIQC-SEGKIYQQCGSPCNQTCRSLSHPDVDCREFCMEGCYCPPGLYTNEDG-ECVPKSDCSCYYDGELFQPDDVFSNHYSMCYCENGLMHCSTSDVPSGYF-PESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGC-ISGCTCPDGMVRHKSKCMFPQRCPCFHAGKEYAPGSSV---EIDC-NTCVCENRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGTFRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHI-----KKPLSDETSFDVLKS-GNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADAAKFGAVSPLSLCKDNLMKQMAAENACNILMGDLFAECAKVVNPEPYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTC-----QHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDC----PVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC 1239 HSP 2 Score: 196.052 bits (497), Expect = 2.434e-49 Identity = 147/492 (29.88%), Postives = 221/492 (44.92%), Query Frame = 2
Query: 1340 GSNCGKNCGQLGMELECESQ-CLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCE--SDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK---------YNQCGSRCTSTCRLLQESKCKEECIEGC--FCPEGETFDEAAKECVKISDCK-CEF------HGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
G C K C ELEC S C+ GC CP + C+ ++CPC E Y G ++++C TC+C + KW+C EN C+ C G HY+TFD + F G C+Y V I + N C + E C + I ++ E + L++ +I + K L D+ S D+L+ L + + W+ G R+ + L + YR+KV G+CGNF+G E +D ++ E D F SWK NP C + G V C + A C+ L + F +C N + +++ CT+D CTC + A C C++ ++YA CA IN+ WR++ LCP+ C +N + YN C + C TC+ + C +C+EGC +CP G+ DE ++ C++ DC C HGR + RC C C N C N CE
Sbjct: 790 GIECAKTCQNY--ELECVSHGCISGCTCPDGMVRHKSKCMFPQRCPCFHAGKE--YAPGSSVEIDCNTCVCE-NRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGV------TGTFRILVGNEGCGFSGEK-CTKRITILFRNGEIE-----LTNEQIHIK--------------KPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADAAKFGAVSPLSLCKDNLMKQMAAENACNILM-GDLFAECAKVVNPEPYWEICTYDTCTCESAGDCA---CFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC--NACE 1243 HSP 3 Score: 140.969 bits (354), Expect = 1.285e-32 Identity = 97/365 (26.58%), Postives = 174/365 (47.67%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPD-REPWAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYE-IGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +GE H ++FDG +++F G CSY+++ D C +F L+++ + K +YL F F+ G+ ++ + P + S+ + + G VK D + ++++++P K+ NK CGLCGN++ ++D + G + ++ +FA+SW +H C P C+ + E +C++LK S +F +CH +V+ + Y C D CEC ++ C C +Y C ++ I W T+ C C + C + C +C +++ N C++ CI+GC CPDG + C+ C C+ + +Y G + R+C C C GA+ C+
Sbjct: 41 CSLFGENHIQTFDGTFYDFYGDCSYMLAGD-CNKH--SFSLLAD-------YRNGKKKSVSLYLGEYFDIHLFLDGTATQGEKHISMPYAS-NGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTC----QSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIH--SGKRYPPGSAIRRDCNSCTCQNGAWECS 386 HSP 4 Score: 99.7525 bits (247), Expect = 4.444e-20 Identity = 111/432 (25.69%), Postives = 172/432 (39.81%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTA---FYWTSDVD-THFILFTMEGIQVKWDKAMSLEIMI--PEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDR---EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVDPITNLKYNTGDKVIR-------ECEECNCYEG------AFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDG 4249
C +C H +FDG F+ G+CSYV+ D ++ K+I N C ++ +C KS++ + + + SN VS N + P P A F T + H + G + A + ++ P + + GLCG D ND G + D F W V S C +PD+ +P + ++C+I+ S +F+ CH + + Y+ C +C G D C + YV C + + WRT + CP C V+ +C C C GK + + T C EGCFCP+G N +CV K C +C D +Y +++ C C C E A C + C P + C +YE +C C N + C +G
Sbjct: 1944 CPCMCMGSSTRHIVTFDGLDFKLMGACSYVLFHD----EVHDVKVILHNAECNTTNHQTCMKSVEVKH--NKYSILLSSNMQVSVNGDIVPL-PYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLG------RTCETKPDKACTQPIS-SECKIMLSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKEC---GKTL-NGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTD-----RYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTC--DVNPVPHCENG 2344 HSP 5 Score: 93.2041 bits (230), Expect = 5.152e-18 Identity = 115/504 (22.82%), Postives = 194/504 (38.49%), Query Frame = 2
Query: 1523 KLNCETCICGM-HGKWQCIENEE-------CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS-KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH-------KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAA----KECVKISDCKCEFHGRF--YLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGIC---------QKTCTNLDLVCPKGQCQ---LPGCFCPENTVLHN-GECIKPFECPCYM 2917
K+NCE M H ++ EE C C S H +TFD F G C Y +L + + + + + N C + C +++ V ++ +I L S+ ++ VN + LPY + L ++ ++ ++ V + ++L+ + + +G+CG + N ++D G D KF+ W +V+ +L R+ +K C K+ S+ F+ CH+ +F C C H + CE ++Y C + + ++WRT + CP +CP M Y+ C C C + L + C + EGCFCPEG+ K C + +D E H ++P C+ C C I CT+ C ++ CG C ++ C + + VC C +P C VL N G C +EC C +
Sbjct: 1914 KVNCEKIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSY----------VLFHDEVHDVKVILHNAECNTTNHQTCMKSVEV----KHNKYSILLSSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEV-KIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWT----------VVD-----SLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASC-----------HGQDICEVIAAYVHVCRINGVCINWRTPDFCPMECPRL-MVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIP---TNAPCSICMCLENRMINCTAMPC-------PTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTCDVNPVPHCENGLVPVLTNPGHCKPNYECACRI 2365 HSP 6 Score: 55.8398 bits (133), Expect = 9.489e-7 Identity = 67/335 (20.00%), Postives = 119/335 (35.52%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD----RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKC 1471
HI TFDG F Y LF D + + + NA + + +T C S+++ +I+L N V + +P+ ++ V+ G + I L +F + + P + GLCG + +D+ + + + F+ W D ++ R E P A Q + C+ M+ C + Y +C++ C C++ Y + C + G+ ++WR+ C +C + C C K CG+ C GC+CP CV ++ C
Sbjct: 1955 HIVTFDGLDFKLMGACSYVLFHDEVHDVKV--ILHNAECNTTNHQT---CMKSVEVKHNKYSILL-SSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVD----SLGRTCETKPDKACT-------QPISSECKIMLSKTF-EACHTIFPPKPYFTVCEEASCH--------------GQDICEVIAAYVHVCRINGVCINWRTP-DFCPM-ECPRLMVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVC 2255
BLAST of von Willebrand factor vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 561.992 bits (1447), Expect = 3.925e-165 Identity = 389/1277 (30.46%), Postives = 600/1277 (46.99%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD-LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP-CNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSM---------AWFDC-----------------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV--------WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNT---GDKVI------RECEECNCYEGAFRC 4102
+S CS+ +HI+TFDG + + Y L DC + F+ D + K + S+ + +G I + A +P+ S GI + S + K+ + + D N+ V P K CGLCG +N DD+ ++ +N F NSW R R YP N + + + + CQ + ++ C V D Y+ +C+D++CEC + NC C+ +Y+ C G+ TICK P C G E++EC S C K C L + C+ QC+ GC CP + DG C+P +C C+ Y G ++ +C +C C +G W+C NE+C G CF++G H+ +FD +HF F G C+Y +L +++ + S+TI+ C D+ + C R+ + + D + I L + ++ I LP G ++ +S +RL + +++ W+G ++ IKLS Y ++G+CGN+NGN+ DD +T G+ E ++ F SWK N C + + C L+ + ++ +VC L +S F+ CH+ N + C +DVC+C NG C C A S+YA C+ + I + WRT E CP +C +E Y QCGS C TCR L + C+E C+EGC+CP G +E ECV SDC C + G + P ++ + + C C NG C+++D F P+S DC GI C KTC N +L C C + GC CP+ V H +C+ P CPC+ K Y+ ++ +C N C+C++ KW C +N C C G +HY +FDG ++F G C YV+++D+CG GTF+++ N CG S CTK I ++ N + I P T+F +FIL + V WDK M + + + E Y++KVCGLCGNFD +ND+ + + +IE D VNF +SWKV+ C P +C ++ A+ C IL D F +C VVN E Y++ C D C C+ DC CFC ++ Y C + I + WR++ CP +C K W+ +C CP +C + C C+EGC +CP G L + C+E K C P+ ++ +T G K+I + C+ C+C +C
Sbjct: 24 WSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCNKHS------FSLLADYRNGKKK-----SVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHG----GARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEEL-------NCP---CQTFLEYARTCAQHGVITSNWPTHTICK-PLCPFGMEYNECVSPCVKTCQSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKR--YPPGSAIRRDCNSCTC-QNGAWEC-SNEDCPGECFVTGQSHFKSFDNKHFTFSGICQY---------LLATDAMDSTFSVTIETVQCADDIDAVCTRSASLRL-LDMHNITIKLKHGGGVSLDGQDILLPLVQ-GFLRIQTTVLSA--VRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTD--LMKQDTDPCNLNPKRARFSEEVCFALMDSA-FQSCHNEVNPAPYLKNCRYDVCSCS---NGK--ECLCSALSTYAAACSRKGILIDWRTPEFCPIQC-SEGKIYQQCGSPCNQTCRSLSHPDVDCREFCMEGCYCPPGLYTNEDG-ECVPKSDCSCYYDGELFQPDDVFSNHYSMCYCENGLMHCSTSDVPSGYF-PESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGC-ISGCTCPDGMVRHKSKCMFPQRCPCFHAGKEYAPGSSV---EIDC-NTCVCENRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGTFRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHI-----KKPLSDETSFDVLKS-GNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSPLSLCKDNLMKQMAAENACNILMGDLFAECAKVVNPEPYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTC-----QHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDC----PVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC 1225 HSP 2 Score: 199.134 bits (505), Expect = 2.965e-50 Identity = 148/490 (30.20%), Postives = 222/490 (45.31%), Query Frame = 2
Query: 1340 GSNCGKNCGQLGMELECESQ-CLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK---------YNQCGSRCTSTCRLLQESKCKEECIEGC--FCPEGETFDEAAKECVKISDCK-CEF------HGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
G C K C ELEC S C+ GC CP + C+ ++CPC E Y G ++++C TC+C + KW+C EN C+ C G HY+TFD + F G C+Y V I + N C + E C + I ++ E + L++ +I + K L D+ S D+L+ L + + W+ G R+ + L + YR+KV G+CGNF+G E +D ++ E D F SWK NP C +D G V C + A C+ L + F +C N + +++ CT+D CTC + A C C++ ++YA CA IN+ WR++ LCP+ C +N + YN C + C TC+ + C +C+EGC +CP G+ DE ++ C++ DC C HGR + RC C C N C N CE
Sbjct: 779 GIECAKTCQNY--ELECVSHGCISGCTCPDGMVRHKSKCMFPQRCPCFHAGKE--YAPGSSVEIDCNTCVCE-NRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGV------TGTFRILVGNEGCGFSGEK-CTKRITILFRNGEIE-----LTNEQIHIK--------------KPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLC-ADFGAVSPLSLCKDNLMKQMAAENACNILM-GDLFAECAKVVNPEPYWEICTYDTCTCESAGDCA---CFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC--NACE 1229 HSP 3 Score: 140.969 bits (354), Expect = 1.315e-32 Identity = 98/365 (26.85%), Postives = 176/365 (48.22%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPD-REPWAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYE-IGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +GE H ++FDG +++F G CSY+++ D C +F L+++ + KS+ +YL F F+ G+ ++ + P + S+ + + G VK D + ++++++P K+ NK CGLCGN++ ++D + G + ++ +FA+SW +H C P C+ + E +C++LK S +F +CH +V+ + Y C D CEC ++ C C +Y C ++ I W T+ C C + C + C +C +++ N C++ CI+GC CPDG + C+ C C+ + +Y G + R+C C C GA+ C+
Sbjct: 30 CSLFGENHIQTFDGTFYDFYGDCSYMLAGD-CNKH--SFSLLAD------YRNGKKKSVS-LYLGEYFDIHLFLDGTATQGEKHISMPYAS-NGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTC----QSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIH--SGKRYPPGSAIRRDCNSCTCQNGAWECS 375 HSP 4 Score: 99.7525 bits (247), Expect = 4.439e-20 Identity = 111/432 (25.69%), Postives = 172/432 (39.81%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTA---FYWTSDVD-THFILFTMEGIQVKWDKAMSLEIMI--PEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDR---EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVDPITNLKYNTGDKVIR-------ECEECNCYEG------AFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDG 4249
C +C H +FDG F+ G+CSYV+ D ++ K+I N C ++ +C KS++ + + + SN VS N + P P A F T + H + G + A + ++ P + + GLCG D ND G + D F W V S C +PD+ +P + ++C+I+ S +F+ CH + + Y+ C +C G D C + YV C + + WRT + CP C V+ +C C C GK + + T C EGCFCP+G N +CV K C +C D +Y +++ C C C E A C + C P + C +YE +C C N + C +G
Sbjct: 1928 CPCMCMGSSTRHIVTFDGLDFKLMGACSYVLFHD----EVHDVKVILHNAECNTTNHQTCMKSVEVKH--NKYSILLSSNMQVSVNGDIVPL-PYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLG------RTCETKPDKACTQPIS-SECKIMLSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKEC---GKTL-NGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTD-----RYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTC--DVNPVPHCENG 2328 HSP 5 Score: 92.8189 bits (229), Expect = 5.190e-18 Identity = 115/504 (22.82%), Postives = 194/504 (38.49%), Query Frame = 2
Query: 1523 KLNCETCICGM-HGKWQCIENEE-------CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS-KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH-------KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAA----KECVKISDCKCEFHGRF--YLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGIC---------QKTCTNLDLVCPKGQCQ---LPGCFCPENTVLHN-GECIKPFECPCYM 2917
K+NCE M H ++ EE C C S H +TFD F G C Y +L + + + + + N C + C +++ V ++ +I L S+ ++ VN + LPY + L ++ ++ ++ V + ++L+ + + +G+CG + N ++D G D KF+ W +V+ +L R+ +K C K+ S+ F+ CH+ +F C C H + CE ++Y C + + ++WRT + CP +CP M Y+ C C C + L + C + EGCFCPEG+ K C + +D E H ++P C+ C C I CT+ C ++ CG C ++ C + + VC C +P C VL N G C +EC C +
Sbjct: 1898 KVNCEKIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSY----------VLFHDEVHDVKVILHNAECNTTNHQTCMKSVEV----KHNKYSILLSSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEV-KIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWT----------VVD-----SLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASC-----------HGQDICEVIAAYVHVCRINGVCINWRTPDFCPMECPRL-MVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIP---TNAPCSICMCLENRMINCTAMPC-------PTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTCDVNPVPHCENGLVPVLTNPGHCKPNYECACRI 2349 HSP 6 Score: 55.8398 bits (133), Expect = 9.404e-7 Identity = 67/335 (20.00%), Postives = 119/335 (35.52%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD----RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKC 1471
HI TFDG F Y LF D + + + NA + + +T C S+++ +I+L N V + +P+ ++ V+ G + I L +F + + P + GLCG + +D+ + + + F+ W D ++ R E P A Q + C+ M+ C + Y +C++ C C++ Y + C + G+ ++WR+ C +C + C C K CG+ C GC+CP CV ++ C
Sbjct: 1939 HIVTFDGLDFKLMGACSYVLFHDEVHDVKV--ILHNAECNTTNHQT---CMKSVEVKHNKYSILL-SSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVD----SLGRTCETKPDKACT-------QPISSECKIMLSKTF-EACHTIFPPKPYFTVCEEASCH--------------GQDICEVIAAYVHVCRINGVCINWRTP-DFCPM-ECPRLMVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVC 2239
BLAST of von Willebrand factor vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 558.14 bits (1437), Expect = 7.892e-164 Identity = 391/1281 (30.52%), Postives = 599/1281 (46.76%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDD-LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP----CNALKLKNNDQYQKALTL-CQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSM---------AWFDC-----------------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV--------WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNT---GDKVI------RECEECNCYEGAFRC 4102
+S CS+ +HI+TFDG + + Y L DC + F+ D + K + S+ + +G I + A +P+ S GI + S + K+ + + D N+ V P K CGLCG +N DD+ ++ +N F NSW R R YP CN Y + CQ + ++ C V D Y+ +C+D++CEC + NC C+ +Y+ C G+ TICK P C G E++EC S C K C L + C+ QC+ GC CP + DG C+P +C C+ Y G ++ +C +C C +G W+C NE+C G CF++G H+ +FD +HF F G C+Y +L +++ + S+TI+ C D+ + C R+ + + D + I L + ++ I LP G ++ +S +RL + +++ W+G ++ IKLS Y ++G+CGN+NGN+ DD +T G+ E ++ F SWK N C + + C L+ + ++ +VC L +S F+ CH+ N + C +DVC+C NG C C A S+YA C+ + I + WRT E CP +C +E Y QCGS C TCR L + C+E C+EGC+CP G +E ECV SDC C + G + P ++ + + C C NG C+++D F P+S DC GI C KTC N +L C C + GC CP+ V H +C+ P CPC+ K Y+ ++ +C N C+C++ KW C +N C C G +HY +FDG ++F G C YV+++D+CG GTF+++ N CG S CTK I ++ N + I P T+F +FIL + V WDK M + + + E Y++KVCGLCGNFD +ND+ + + +IE D VNF +SWKV+ C P +C ++ A+ C IL D F +C VVN E Y++ C D C C+ DC CFC ++ Y C + I + WR++ CP +C K W+ +C CP +C + C C+EGC +CP G L + C+E K C P+ ++ +T G K+I + C+ C+C +C
Sbjct: 18 WSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCNKHS------FSLLADYRNGKKK-----SVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHG----GARRCRRTYPPSNTCNISSETAEKVYVGLWKVECQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEEL-------NCP---CQTFLEYARTCAQHGVITSNWPTHTICK-PLCPFGMEYNECVSPCVKTCQSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKR--YPPGSAIRRDCNSCTC-QNGAWEC-SNEDCPGECFVTGQSHFKSFDNKHFTFSGICQY---------LLATDAMDSTFSVTIETVQCADDIDAVCTRSASLRL-LDMHNITIKLKHGGGVSLDGQDILLPLVQ-GFLRIQTTVLSA--VRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTD--LMKQDTDPCNLNPKRARFSEEVCFALMDSA-FQSCHNEVNPAPYLKNCRYDVCSCS---NGK--ECLCSALSTYAAACSRKGILIDWRTPEFCPIQC-SEGKIYQQCGSPCNQTCRSLSHPDVDCREFCMEGCYCPPGLYTNEDG-ECVPKSDCSCYYDGELFQPDDVFSNHYSMCYCENGLMHCSTSDVPSGYF-PESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGC-ISGCTCPDGMVRHKSKCMFPQRCPCFHAGKEYAPGSSV---EIDC-NTCVCENRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGTFRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHI-----KKPLSDETSFDVLKS-GNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSPLSLCKDNLMKQMAAENACNILMGDLFAECAKVVNPEPYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTC-----QHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDC----PVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC 1223 HSP 2 Score: 199.134 bits (505), Expect = 2.989e-50 Identity = 148/490 (30.20%), Postives = 222/490 (45.31%), Query Frame = 2
Query: 1340 GSNCGKNCGQLGMELECESQ-CLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK---------YNQCGSRCTSTCRLLQESKCKEECIEGC--FCPEGETFDEAAKECVKISDCK-CEF------HGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCE 2734
G C K C ELEC S C+ GC CP + C+ ++CPC E Y G ++++C TC+C + KW+C EN C+ C G HY+TFD + F G C+Y V I + N C + E C + I ++ E + L++ +I + K L D+ S D+L+ L + + W+ G R+ + L + YR+KV G+CGNF+G E +D ++ E D F SWK NP C +D G V C + A C+ L + F +C N + +++ CT+D CTC + A C C++ ++YA CA IN+ WR++ LCP+ C +N + YN C + C TC+ + C +C+EGC +CP G+ DE ++ C++ DC C HGR + RC C C N C N CE
Sbjct: 777 GIECAKTCQNY--ELECVSHGCISGCTCPDGMVRHKSKCMFPQRCPCFHAGKE--YAPGSSVEIDCNTCVCE-NRKWKCTENI-CDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGV------TGTFRILVGNEGCGFSGEK-CTKRITILFRNGEIE-----LTNEQIHIK--------------KPLSDETSFDVLKSGNYFILLLGNQMSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLC-ADFGAVSPLSLCKDNLMKQMAAENACNILM-GDLFAECAKVVNPEPYWEICTYDTCTCESAGDCA---CFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQHPEPVNCPLKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQC--NACE 1227 HSP 3 Score: 140.584 bits (353), Expect = 1.810e-32 Identity = 98/370 (26.49%), Postives = 175/370 (47.30%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMCSKRPDREP------WAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYE-IGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +GE H ++FDG +++F G CSY+++ D C +F L+++ + K +YL F F+ G+ ++ + P + S+ + + G VK D + ++++++P K+ NK CGLCGN++ ++D + G + ++ +FA+SW +H C P C+ + W K +C++LK S +F +CH +V+ + Y C D CEC ++ C C +Y C ++ I W T+ C C + C + C +C +++ N C++ CI+GC CPDG + C+ C C+ + +Y G + R+C C C GA+ C+
Sbjct: 24 CSLFGENHIQTFDGTFYDFYGDCSYMLAGD-CNKH--SFSLLAD-------YRNGKKKSVSLYLGEYFDIHLFLDGTATQGEKHISMPYAS-NGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKVYVGLW-KVECQMLKTSSAFMQCHHLVDPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTC----QSLNINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIH--SGKRYPPGSAIRRDCNSCTCQNGAWECS 373 HSP 4 Score: 99.7525 bits (247), Expect = 4.364e-20 Identity = 111/432 (25.69%), Postives = 172/432 (39.81%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTA---FYWTSDVD-THFILFTMEGIQVKWDKAMSLEIMI--PEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDR---EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVDPITNLKYNTGDKVIR-------ECEECNCYEG------AFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDG 4249
C +C H +FDG F+ G+CSYV+ D ++ K+I N C ++ +C KS++ + + + SN VS N + P P A F T + H + G + A + ++ P + + GLCG D ND G + D F W V S C +PD+ +P + ++C+I+ S +F+ CH + + Y+ C +C G D C + YV C + + WRT + CP C V+ +C C C GK + + T C EGCFCP+G N +CV K C +C D +Y +++ C C C E A C + C P + C +YE +C C N + C +G
Sbjct: 1926 CPCMCMGSSTRHIVTFDGLDFKLMGACSYVLFHD----EVHDVKVILHNAECNTTNHQTCMKSVEVKH--NKYSILLSSNMQVSVNGDIVPL-PYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLG------RTCETKPDKACTQPIS-SECKIMLSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKEC---GKTL-NGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTD-----RYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTC--DVNPVPHCENG 2326 HSP 5 Score: 93.2041 bits (230), Expect = 5.060e-18 Identity = 115/504 (22.82%), Postives = 194/504 (38.49%), Query Frame = 2
Query: 1523 KLNCETCICGM-HGKWQCIENEE-------CEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS-KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH-------KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAA----KECVKISDCKCEFHGRF--YLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGIC---------QKTCTNLDLVCPKGQCQ---LPGCFCPENTVLHN-GECIKPFECPCYM 2917
K+NCE M H ++ EE C C S H +TFD F G C Y +L + + + + + N C + C +++ V ++ +I L S+ ++ VN + LPY + L ++ ++ ++ V + ++L+ + + +G+CG + N ++D G D KF+ W +V+ +L R+ +K C K+ S+ F+ CH+ +F C C H + CE ++Y C + + ++WRT + CP +CP M Y+ C C C + L + C + EGCFCPEG+ K C + +D E H ++P C+ C C I CT+ C ++ CG C ++ C + + VC C +P C VL N G C +EC C +
Sbjct: 1896 KVNCEKIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSY----------VLFHDEVHDVKVILHNAECNTTNHQTCMKSVEV----KHNKYSILLSSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEV-KIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWT----------VVD-----SLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASC-----------HGQDICEVIAAYVHVCRINGVCINWRTPDFCPMECPRL-MVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIP---TNAPCSICMCLENRMINCTAMPC-------PTVKPLTCGPCEIPRLKKTSEQCCPDYECVCDLTTCDVNPVPHCENGLVPVLTNPGHCKPNYECACRI 2347 HSP 6 Score: 55.8398 bits (133), Expect = 9.169e-7 Identity = 67/335 (20.00%), Postives = 119/335 (35.52%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD----RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKC 1471
HI TFDG F Y LF D + + + NA + + +T C S+++ +I+L N V + +P+ ++ V+ G + I L +F + + P + GLCG + +D+ + + + F+ W D ++ R E P A Q + C+ M+ C + Y +C++ C C++ Y + C + G+ ++WR+ C +C + C C K CG+ C GC+CP CV ++ C
Sbjct: 1937 HIVTFDGLDFKLMGACSYVLFHDEVHDVKV--ILHNAECNTTNHQT---CMKSVEVKHNKYSILL-SSNMQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVD----SLGRTCETKPDKACT-------QPISSECKIMLSKTF-EACHTIFPPKPYFTVCEEASCH--------------GQDICEVIAAYVHVCRINGVCINWRTP-DFCPM-ECPRLMVYHHCHMGCNKECGKTLNGTLCSDSSTEGCFCPEGDVIVNGKCVNEKVC 2237
BLAST of von Willebrand factor vs. Ensembl Mouse
Match: Vwf (Von Willebrand factor [Source:MGI Symbol;Acc:MGI:98941]) HSP 1 Score: 514.227 bits (1323), Expect = 4.312e-149 Identity = 357/1264 (28.24%), Postives = 549/1264 (43.43%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKG-IDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG---------------------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQK-----PPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKN----------CGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVI------RECEECNC 4081
CS+ I TFD ++S+ Y L DC + F + N Q+ +S+ + +G I N + +P+ S+G+ + + K+ D + N V K CGLCG FN DD+R + + F NSW S +R P + + D +Q CQ + ++ C V + ++ +C+ +C C T PE + C + +Y+ C +G+ + + + C+ P C G E+ EC S C + C L + C+ QC+ GC CP D CV CPCV + Y G L +C TCIC + W C NEEC G C ++G H+ +FD R+F F G C+Y D+ SI I+ C D+ + C R++ V + + + + L + ++ + LP+ G+ + I +RL +++ W+G R+ +KLS Y K G+CGN+NGN+ DD +T G+ E + F +WK C R ++ C+L+ + +A + C L S +F+ CH A + + C +DVC+C R+ C C A ++YA +CA + +++ WR C CP + Y QCG+ C TCR L + +C E C+EGC+CP G DE +CV + C C + G + P +I D C C +G CT++ G K CP C KTC N DL C C + GC CP V H +C+ CPC+ Y+ DT+ G N C+C++ KW C N C C G HY +FDG + F G C YV+ +D+CG+ GTF+++ N C S C K + + + G + P + + + + IL + + V WD +S+ +++ YQ +VCGLCGNFD +ND S ++E+D VNF +SWKV S C +K P C ++ + C IL SD F+ C+ +V+ E Y C+ D C C+ DC CFC + Y C ++ + WRT CP +C K V +N C CP +C + C C+EGC CP G L CV+ + C + + + G K+ C+ C+C
Sbjct: 34 RCSLFGDDFINTFDETMYSFAGGCSYLLAGDCQKRS--FSILGN---------FQDGKRMSLSVYLGEFFDIHLFANGTVTQGDQSISMPYASQGLYLEREAGYYKLSSETFGFAARIDGNGNFQVLMSDRHFNKTCGLCGDFNIFAEDDFRTQEGTLTSDPYDFANSWAL---SSEEQRCKRASPPSRNCESSSGDMHQAMWEQCQLLKTASVFARCHPLVDPESFVALCEKILCTC-ATGPECA---------CPVLLEYARTCAQEGMVLYGWTDHSACR-PACPAGMEYKECVSPCPRTCQSLSINEVCQQQCVDGCSCPEGELLDEDRCVQSSDCPCV--HAGKRYPPGTSLSQDCNTCIC-RNSLWIC-SNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCEDHT---------FSIVIETMQCADDPDAVCTRSVSVRLSALHNSL-VKLKHGGAVGIDGQDVQLPFLQ-GDLR--IQHTVMASVRLSYAEDLQMDWDGRGRLLVKLSPVYSGKTCGLCGNYNGNKGDDFLTPAGLVEPLVVDFGNAWKLQGDCSDLRR--QHSDPCSLNPRLTRFAEEAC-ALLTSSKFEACHHAVSPLPYLQNCRYDVCSCSDSRD-----CLCNAVANYAAECARKGVHIGWREPGFCALGCP-QGQVYLQCGNSCNLTCRSLSLPDEECSEVCLEGCYCPPGLYQDERG-DCVPKAQCPCYYDGELFQPADIFSDHHTMCYCEDGFMHCTTSGTLGSLLPDTVLSSPLSHRSKRSLSCRPPMVKLVCPADNPRAQGLECAKTCQNYDLECMSLGC-VSGCLCPPGMVRHENKCVALERCPCFHQGAEYAPGDTVKIG----CNTCVCRERKWNC-TNHVCDATCSAIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFQILVGNEGCSYPSVKCRKRVTILVDGGELELFDGEVNVKR------PLRDESHFEVVESGRYVILLLGQALSVVWDHHLSISVVLKHTYQEQVCGLCGNFDGIQNNDFTTSSLQVEEDPVNFGNSWKVSSQCADTRKLSLDVSPATCHNNIMKQTMVDSACRILTSDVFQGCNRLVDPEPYLDICIYDTCSCESIGDCACFCDTIAAYAHVCAQHGQVVAWRTPTLCPQSCEEKNVRENGYECEWRYNSCAPACPVTC-----QHPEPLACPVQCVEGCHAHCPPGRILDELLQTCVDPQDCPVCE-VAGRRLAPGKKITLSPDDPAHCQNCHC 1227 HSP 2 Score: 127.102 bits (318), Expect = 1.951e-28 Identity = 90/372 (24.19%), Postives = 166/372 (44.62%), Query Frame = 2
Query: 3014 QNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNY--PPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMC-SKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
+++ + C +G+ +FD + F G CSY+++ D C + +F ++ N G + +YL F +N V++ P Y + + + G + D + ++++ +++ NK CGLCG+F++ ++D R + G + D +FA+SW + S C P C S D +C++LK+ S F +CH +V+ E + C C C G +C C + +Y C + + L W + C C +K C + CP +C ++++ N C++ C++GC CP+G + CV+ C CV +Y G + ++C C C + C+
Sbjct: 27 RDRPSTARCSLFGDDFINTFDETMYSFAGGCSYLLAGD-CQKR--SFSILG-NFQDGK------RMSLSVYLGEFFDIHLFANGTVTQGDQSISMPYASQGLYLEREAGYYKLSSETFGFAARIDGNGNFQVLMSDRHFNKTCGLCGDFNIFAEDDFRTQEGTLTSDPYDFANSWALSSEEQRCKRASPPSRNCESSSGDMHQAMWEQCQLLKTASVFARCHPLVDPESFVALCEKILCTCATGPECAC--PVLLEYARTCAQEGMVLYGWTDHSACRPACPAGMEYKECVSPCPRTC----QSLSINEVCQQQCVDGCSCPEGELLDEDRCVQSSDCPCVH--AGKRYPPGTSLSQDCNTCICRNSLWICS 380 HSP 3 Score: 105.916 bits (263), Expect = 5.357e-22 Identity = 99/392 (25.26%), Postives = 157/392 (40.05%), Query Frame = 2
Query: 2963 ANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGX-XXXXCTKSIKFIYLNRVFQF-------VRGS---NPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDRE-PWA-KAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV--DPITNLKYNTGDKVIRECEECNCYEG 4090
AN Q+P ++ C C +C H +FDG+ F+ GSCSYVI + NK +++ N AC + +C KSI+ + + V G P V N G + H + +T + + + + P+ + +K+ GLCG D ND R G + D W V Q+P + C P+ + P + + C++L S SF +CH V+ ++ C D+C +R C + Y C + + WRT + C +C V+ +C CP C + + C EGCFCP + CV +AC +CV D + + T + C+ C C G
Sbjct: 1928 ANSQSPVRVEE---TCGCRWTCPCVCTGSSTRHIVTFDGQNFKLTGSCSYVIFQ----NKEQDLEVLLHNGACSPGAKQACMKSIEIKHAGVSAELHSNMEMAVDGRLVLAPYVGENMEVSIYGAIMYEVRFTHLGHILTYTPQNNEFQLQ-------LSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTV-------QQPGYTCQAVPEEQCPVSDSSHCQVLLSASFAECHKVIAPATFHTICQQDSCHQER------VCEVIASYAHLCRTSGVCVDWRTTDFCAMSCPPSLVYNHCERGCPRHCDG------NTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDGVRHQFLETWVPDHQPCQICMCLSG 2286 HSP 4 Score: 93.2041 bits (230), Expect = 3.697e-18 Identity = 116/461 (25.16%), Postives = 176/461 (38.18%), Query Frame = 2
Query: 1601 CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETF-------DEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ----KTCTNL-----DLVCPKGQCQLP------GCFCPENTVLHNGECIKPFECPC 2911
C S H +TFD ++F G C Y + L + + N C ++ C ++I + V+ L S+ E+ V+ + PY N +V I +R + + +++LS K + K+ G+CG + N +D G D + V W + + + E + C +S S C L S F +CH F C D +C +R CE +SYA C + + WRT + C CP ++ YN C C C S C + EGCFCP+ + F +EA +CV + +F + ++ C C C +G I CT+ CP + A CG C+ K TNL + VC C LP G P T+ + GEC F C C
Sbjct: 1950 CTGSSTRHIVTFDGQNFKLTGSCSYVIFQNKEQD----------LEVLLHNGACSPGAKQACMKSIEI----KHAGVSAELHSNMEMAVDGRLVLAPYVGE-NMEVSIYGAIMYEVRFTHLGHILTYTPQNNEFQLQLSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTVQQPGYTCQAVPEEQ--CPVSDSSH------CQVLL-SASFAECHKVIAPATFHTICQQD--SCHQER-------VCEVIASYAHLCRTSGVCVDWRTTDFCAMSCP-PSLVYNHCERGCPRHCD-GNTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDGVRHQFLETWVPDHQ----PCQICMCLSGRKINCTAQP------CPTARAP-TCGPCEVARLKQSTNLCCPEYECVCDLFNCNLPPVPPCEGGLQP--TLTNPGECRPTFTCAC 2362 HSP 5 Score: 60.8474 bits (146), Expect = 2.213e-8 Identity = 76/350 (21.71%), Postives = 128/350 (36.57%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVY-----------APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCP-CVRDNG 1492
HI TFDG F Y +F + L + N + + C S++I + L E A V P+ E + VS G+ + E+ F +I Y +P T K+ GLCG + ++D+ + + + K V W + P Y C A+ + + CQ ++ + C + + +CQ + C C++ Y++ C G+ VDWR+ T C C ++ C C ++C G C GC+CP+++ + CVP+E C CV ++G
Sbjct: 1957 HIVTFDGQNFKLTGSCSYVIFQN--KEQDLEVLLHNGA---CSPGAKQACMKSIEIKHAGVSAELHSNMEMA-VDGRLVLAPYVGENMEVSIYGAIM-------YEVRFTHLGHILTYTPQNNEFQLQLSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTVQQPG----------YTCQAVPEEQCPVSDS--SHCQVLLSASFAE-CHKVIAPATFHTICQQDSCH--------------QERVCEVIASYAHLCRTSGVCVDWRT-TDFCAM-SCPPSLVYNHCERGCPRHCD--GNTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDG 2262
BLAST of von Willebrand factor vs. Ensembl Mouse
Match: Muc5ac (mucin 5, subtypes A and C, tracheobronchial/gastric [Source:MGI Symbol;Acc:MGI:104697]) HSP 1 Score: 489.96 bits (1260), Expect = 4.530e-141 Identity = 372/1281 (29.04%), Postives = 563/1281 (43.95%), Query Frame = 2
Query: 413 AVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGV--EILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLY-ESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP---VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCID-IGKNVTDNTGCKKVCIEGCF--CP-------DGLHWQNNECVERKACECVDPITNLKYNTGDKVI--RECEECNCYEGAFRCT 4105
V R N + T CS H KTFDG VF +P Y C A Y FN + +++ + ++T+ +++ LV N+ ++PF+ G+++ ++K+ L +++ D ++ + K CGLCG FNG N+ + NV+ + + D + ++ P + KN +C+ ++K L C + V I Y++ C+ ++C C P + C +YS C G DWR +C Q C + ECGS C C CE C+ GC+CP D CVP +C C+ + + Y G +C C C G+W C ++ C G C + G H TFD R + G C Y N ++ ++ C C +T+ + +G + ++ + ++ E+ VN + LP T N + ST + ++ G+ EI + + ++L R G+CGNFN + DD T GV E F ++KT C + + I ++ C+LS YA C L S F +CH+ N FF C +D C C + C C A SSY R CA + + LS WR + +C K CP ++M Y S C TCR L E ++GC CP+G D+ K CV+ + C C + G E QD CTCT G C C M +FDC CQK+C LD+ C +C +PGC CP V NG C+ +CPC N Y +TI G C N C C++ W C +K C C +G+ HY +FDG+ + F G C Y + +D CG + F++I+ENI CG++ ++C+KSIK N + +V ++ P Y+ + + ++ T G+ + WDK S+ + + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP VP+ P C+ P R+ WA+ +C I+ S++F CH V YY+ CV DAC CD G DC CFCT V Y C+E + + WRT + CP C G+ W + CG C +C + G+ + D G +EGC+ CP +G + C C + Y G V + C+ C C E RCT
Sbjct: 66 PVVRAFNPGH-TRRVCSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDA----YEDFNIQ---LRRVQESNTTTLSRVTMKLDGLVVELTKSSVLVNNHPVQLPFSQSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKS-----NEFLSNNVRLTPLEFGNLQKMDGPTEQCQDPLP---VPQKNCSARSG---ICEMILKGELFSGCAALVDISSYVEACRQDVCLCESLDPSDC--------ICHTLAEYSRQCAHAGGQPQDWR-GPNLCSQ-TCPLNMQHQECGSPCVDTCSNPQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNG--TLYAPGTNYSTDCTKCTCS-GGQWSC-QDIPCAGTCSVMGGSHMSTFDGRQYTVHGDCTYVLSKPCDSNAF---------TVLVELRKCGLTESETCLKTVTLNLGGGQTEIMVK--ATGEVFVNQIYTQLPVS-TANATFF--RPSTFFIVGETNLGLQLEIQLSPIMQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNI--FQDPCSLSVENEKYAQHWCSLLTNASGPFSQCHATVNPSTFFSNCMYDTCNCEKSED-----CMCAALSSYVRACAAKGVLLSDWR-DGICTKPTITCP-KSMTYQYHISTCQPTCRALNEKDVTCHVSFIPVDGCTCPKGTFLDDLGK-CVQATSCPCYYKGSTVPNGESVQDSGAICTCTQGALTCIGGPAP-TPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCYSSEC-VPGCVCPNGLVADGNGGCVVTEDCPCVHNEATYRPGETIQVG---CNN-CTCENRMWQCT-DKPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLSDSKMEVVQKDVGQEPP-----YFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPDVLVPKDP---CTANPYRKSWAQKQCSIINSETFSACHAHVEPAKYYEACVNDACACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRG-----LEGCYPKCPPTAPIFDEGTMQCVSNCTVTFPCR----VNGKLYRPGASVPSDKNCDSCICTESGVRCT 1265 HSP 2 Score: 201.06 bits (510), Expect = 6.184e-51 Identity = 147/503 (29.22%), Postives = 240/503 (47.71%), Query Frame = 2
Query: 1280 SATTICKQPQCF---NGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM-CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK------YNQCGSRCTSTCR-----LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDC----KCEFHGRFYLP-YEIRQDR-CNNCTCTNGNWICTSN 2725
+ T +C P + + + G+ C K+C L M S+C+PGC CP DG CV E CPCV + E+TY+ G +++ C C C + WQC + + C C + GD HYITFD + + F+G CEYT + DN + S+ + +N C + T C ++I + +G + E+K++ K+ + + G + L ++++ G+ +LW+ + I ++LS +++ +V G+CGNF+ N ++D T D+ +F SWK +P+C ++ K C + +A K C + SE F CH+ K+++ C +D C C +G C C ++YA+ C + +SWRT ++CP C N + Y CG+ C TC+ LQ+ + E C C P FDE +CV S+C C +G+ Y P + D+ C++C CT CT N
Sbjct: 796 APTPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCY-SSECVPGCVCPNGLVADGNGGCVVTEDCPCVHN--EATYRPGETIQVGCNNCTC-ENRMWQCTD-KPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQ---DNCGGNGSSQDAFRVITENIPC-GTTGTTCSKSIKIFLG------------NYELKLSDSKMEVVQKDVGQEPPYFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPD---VLVPKDPCTANPYRKSWAQKQC-SIINSETFSACHAHVEPAKYYEACVNDACAC---DSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRGLEGCYPKCP-PTAPIFDEGTMQCV--SNCTVTFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHN 1267 HSP 3 Score: 113.62 bits (283), Expect = 2.411e-24 Identity = 113/427 (26.46%), Postives = 172/427 (40.28%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPP---------FMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCI---DIGKNVTDNTGCKKVCIEGCFCPDGLH-WQNNECVERKACE-CVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCA 4213
C Q+ QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N C S SC +SI Y R +N I+ N PG TS V + E G++V + + + +P + N G CG ++ R G I + WK VP +P + + CE++ S++FK CHDV+ +Y+ C+ D C D C+ +E Y + C + + WR TN C TC +V++ CG P C I ++T K EGCFCPD + N+ + +C+ C+ P G + +C++C C E C K K C + PE P E CP CA
Sbjct: 2735 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVFGYFRVLIDNYYCDVGDSVSCPQSIIVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNGIVTSRVGIKMYVTIQEIGVRVMF-SGLIFSVEVPFNLFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWK------VPNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYCHM---LDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCPDNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPKT--EGCFCPDSTTLFSTNDSICVPSCQWCLGP-RGEPVEPGHTISIDCQDCICKEATLTCQK---KACPQPTCPEPGFVPVPVALEAGQCCPQFSCA 3144 HSP 4 Score: 92.4337 bits (228), Expect = 6.751e-18 Identity = 120/496 (24.19%), Postives = 175/496 (35.28%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL----SITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFV-----KISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALD-HCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC---------RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKE-----FCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVL----HNGECIKPFECP----CYMNNKMYSENDTI 2950
C ++ +C+ C GDPHYITFD ++ F +C Y V I P+ + I N C CP++I I+ +D+V + S + N + +S +Q G ++ +V + E GV ++++G +E+ + + G CG ++ D+ G + L WK P S +G + S + +L S FK CH +F+ C D C LD C YA CA + + + WR TN C CP +N Y CG C LQE+ K EGCFCP+ T F CV G P C +C C C C F P +A + G C C C C P CP+N+ L G C C C +N +Y D +
Sbjct: 2735 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQ-----------QIVPVFGYFRVLIDNYYCDVGDSVSCPQSI--IVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNG---IVTSRVGIKMYVTIQEIGVRVMFSGLIFSVEVPFN-LFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWKV-PNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYC-------HMLDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCP-DNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPK---TEGCFCPDSTTLFSTNDSICVPSCQWCLGPRGEPVEPGHTISIDCQDCICKEATLTCQKKACPQPTCPEPGFVPVPVA-LEAGQC---CPQFSCACNSSHCP-PPLHCPKNSSLIVTYEEGACCPTQNCSSQKGCEVNGTLYQPGDVV 3196
BLAST of von Willebrand factor vs. Ensembl Mouse
Match: Muc5ac (mucin 5, subtypes A and C, tracheobronchial/gastric [Source:MGI Symbol;Acc:MGI:104697]) HSP 1 Score: 486.108 bits (1250), Expect = 6.431e-140 Identity = 371/1281 (28.96%), Postives = 562/1281 (43.87%), Query Frame = 2
Query: 416 VTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGV--EILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLY-ESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP---VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCID-IGKNVTDNTGCKKVCIEGCF--CP-------DGLHWQNNECVERKACECVDPITNLKYNTGDKVI--RECEECNCYEGAFRCTK 4108
V R N + T CS H KTFDG VF +P Y C A Y FN + +++ + ++T+ +++ LV N+ ++PF+ G+++ ++K+ L +++ D ++ + K CGLCG FNG N+ + NV+ + + D + ++ P + KN +C+ ++K L C + V I Y++ C+ ++C C P + C +YS C G DWR +C Q C + ECGS C C CE C+ GC+CP D CVP +C C+ + + Y G +C G G+W C ++ C G C + G H TFD R + G C Y N ++ ++ C C +T+ + +G + ++ + ++ E+ VN + LP T N + ST + ++ G+ EI + + ++L R G+CGNFN + DD T GV E F ++KT C + + I ++ C+LS YA C L S F +CH+ N FF C +D C C + C C A SSY R CA + + LS WR + +C K CP ++M Y S C TCR L E ++GC CP+G D+ K CV+ + C C + G E QD CTCT G C C M +FDC CQK+C LD+ C +C +PGC CP V NG C+ +CPC N Y +TI G C N C C++ W C +K C C +G+ HY +FDG+ + F G C Y + +D CG + F++I+ENI CG++ ++C+KSIK N + +V ++ P Y+ + + ++ T G+ + WDK S+ + + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP VP+ P C+ P R+ WA+ +C I+ S++F CH V YY+ CV DAC CD G DC CFCT V Y C+E + + WRT + CP C G+ W + CG C +C + G+ + D G +EGC+ CP +G + C C + Y G V + C+ C C E RCT
Sbjct: 67 VVRAFNPGH-TRRVCSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDA----YEDFNIQ---LRRVQESNTTTLSRVTMKLDGLVVELTKSSVLVNNHPVQLPFSQSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKS-----NEFLSNNVRLTPLEFGNLQKMDGPTEQCQDPLP---VPQKNCSARSG---ICEMILKGELFSGCAALVDISSYVEACRQDVCLCESLDPSDC--------ICHTLAEYSRQCAHAGGQPQDWR-GPNLCSQ-TCPLNMQHQECGSPCVDTCSNPQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNG--TLYAPGTNYSTDCTNTCSG--GQWSC-QDIPCAGTCSVMGGSHMSTFDGRQYTVHGDCTYVLSKPCDSNAF---------TVLVELRKCGLTESETCLKTVTLNLGGGQTEIMVK--ATGEVFVNQIYTQLPVS-TANATFF--RPSTFFIVGETNLGLQLEIQLSPIMQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNI--FQDPCSLSVENEKYAQHWCSLLTNASGPFSQCHATVNPSTFFSNCMYDTCNCEKSED-----CMCAALSSYVRACAAKGVLLSDWR-DGICTKPTITCP-KSMTYQYHISTCQPTCRALNEKDVTCHVSFIPVDGCTCPKGTFLDDLGK-CVQATSCPCYYKGSTVPNGESVQDSGAICTCTQGALTCIGGPAP-TPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCYSSEC-VPGCVCPNGLVADGNGGCVVTEDCPCVHNEATYRPGETIQVG---CNN-CTCENRMWQCT-DKPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLSDSKMEVVQKDVGQEPP-----YFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPDVLVPKDP---CTANPYRKSWAQKQCSIINSETFSACHAHVEPAKYYEACVNDACACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRG-----LEGCYPKCPPTAPIFDEGTMQCVSNCTVTFPCR----VNGKLYRPGASVPSDKNCDSCICTESGVRCTH 1265 HSP 2 Score: 201.83 bits (512), Expect = 3.710e-51 Identity = 147/503 (29.22%), Postives = 240/503 (47.71%), Query Frame = 2
Query: 1280 SATTICKQPQCF---NGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM-CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK------YNQCGSRCTSTCR-----LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDC----KCEFHGRFYLP-YEIRQDR-CNNCTCTNGNWICTSN 2725
+ T +C P + + + G+ C K+C L M S+C+PGC CP DG CV E CPCV + E+TY+ G +++ C C C + WQC + + C C + GD HYITFD + + F+G CEYT + DN + S+ + +N C + T C ++I + +G + E+K++ K+ + + G + L ++++ G+ +LW+ + I ++LS +++ +V G+CGNF+ N ++D T D+ +F SWK +P+C ++ K C + +A K C + SE F CH+ K+++ C +D C C +G C C ++YA+ C + +SWRT ++CP C N + Y CG+ C TC+ LQ+ + E C C P FDE +CV S+C C +G+ Y P + D+ C++C CT CT N
Sbjct: 795 APTPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCY-SSECVPGCVCPNGLVADGNGGCVVTEDCPCVHN--EATYRPGETIQVGCNNCTC-ENRMWQCTD-KPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQ---DNCGGNGSSQDAFRVITENIPC-GTTGTTCSKSIKIFLG------------NYELKLSDSKMEVVQKDVGQEPPYFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPD---VLVPKDPCTANPYRKSWAQKQC-SIINSETFSACHAHVEPAKYYEACVNDACAC---DSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRGLEGCYPKCP-PTAPIFDEGTMQCV--SNCTVTFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHN 1266 HSP 3 Score: 114.775 bits (286), Expect = 9.533e-25 Identity = 113/427 (26.46%), Postives = 172/427 (40.28%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPP---------FMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCI---DIGKNVTDNTGCKKVCIEGCFCPDGLH-WQNNECVERKACE-CVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCA 4213
C Q+ QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N C S SC +SI Y R +N I+ N PG TS V + E G++V + + + +P + N G CG ++ R G I + WK VP +P + + CE++ S++FK CHDV+ +Y+ C+ D C D C+ +E Y + C + + WR TN C TC +V++ CG P C I ++T K EGCFCPD + N+ + +C+ C+ P G + +C++C C E C K K C + PE P E CP CA
Sbjct: 2031 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVFGYFRVLIDNYYCDVGDSVSCPQSIIVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNGIVTSRVGIKMYVTIQEIGVRVMF-SGLIFSVEVPFNLFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWK------VPNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYC---HMLDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCPDNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPKT--EGCFCPDSTTLFSTNDSICVPSCQWCLGP-RGEPVEPGHTISIDCQDCICKEATLTCQK---KACPQPTCPEPGFVPVPVALEAGQCCPQFSCA 2440 HSP 4 Score: 93.5893 bits (231), Expect = 2.419e-18 Identity = 118/492 (23.98%), Postives = 172/492 (34.96%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFV-----KISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALD-HCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC---------RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKE-----FCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVL----HNGECIKPFECP----CYMNNKMYSENDTI 2950
C ++ +C+ C GDPHYITFD ++ F +C Y V + I N C CP++I I+ +D+V + S + N + +S +Q G ++ +V + E GV ++++G +E+ + + G CG ++ D+ G + L WK P S +G + S + +L S FK CH +F+ C D C LD C YA CA + + + WR TN C CP +N Y CG C LQE+ K EGCFCP+ T F CV G P C +C C C C F P +A + G C C C C P CP+N+ L G C C C +N +Y D +
Sbjct: 2031 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-------PVFGYFRVLIDNYYCDVGDSVSCPQSI--IVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNG---IVTSRVGIKMYVTIQEIGVRVMFSGLIFSVEVPFN-LFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWKV-PNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYCH-------MLDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCP-DNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPK---TEGCFCPDSTTLFSTNDSICVPSCQWCLGPRGEPVEPGHTISIDCQDCICKEATLTCQKKACPQPTCPEPGFVPVPVA-LEAGQC---CPQFSCACNSSHCP-PPLHCPKNSSLIVTYEEGACCPTQNCSSQKGCEVNGTLYQPGDVV 2492
BLAST of von Willebrand factor vs. Ensembl Mouse
Match: Muc5ac (mucin 5, subtypes A and C, tracheobronchial/gastric [Source:MGI Symbol;Acc:MGI:104697]) HSP 1 Score: 486.108 bits (1250), Expect = 6.431e-140 Identity = 371/1281 (28.96%), Postives = 562/1281 (43.87%), Query Frame = 2
Query: 416 VTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGV--EILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLY-ESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP---VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCID-IGKNVTDNTGCKKVCIEGCF--CP-------DGLHWQNNECVERKACECVDPITNLKYNTGDKVI--RECEECNCYEGAFRCTK 4108
V R N + T CS H KTFDG VF +P Y C A Y FN + +++ + ++T+ +++ LV N+ ++PF+ G+++ ++K+ L +++ D ++ + K CGLCG FNG N+ + NV+ + + D + ++ P + KN +C+ ++K L C + V I Y++ C+ ++C C P + C +YS C G DWR +C Q C + ECGS C C CE C+ GC+CP D CVP +C C+ + + Y G +C G G+W C ++ C G C + G H TFD R + G C Y N ++ ++ C C +T+ + +G + ++ + ++ E+ VN + LP T N + ST + ++ G+ EI + + ++L R G+CGNFN + DD T GV E F ++KT C + + I ++ C+LS YA C L S F +CH+ N FF C +D C C + C C A SSY R CA + + LS WR + +C K CP ++M Y S C TCR L E ++GC CP+G D+ K CV+ + C C + G E QD CTCT G C C M +FDC CQK+C LD+ C +C +PGC CP V NG C+ +CPC N Y +TI G C N C C++ W C +K C C +G+ HY +FDG+ + F G C Y + +D CG + F++I+ENI CG++ ++C+KSIK N + +V ++ P Y+ + + ++ T G+ + WDK S+ + + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP VP+ P C+ P R+ WA+ +C I+ S++F CH V YY+ CV DAC CD G DC CFCT V Y C+E + + WRT + CP C G+ W + CG C +C + G+ + D G +EGC+ CP +G + C C + Y G V + C+ C C E RCT
Sbjct: 67 VVRAFNPGH-TRRVCSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDA----YEDFNIQ---LRRVQESNTTTLSRVTMKLDGLVVELTKSSVLVNNHPVQLPFSQSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKS-----NEFLSNNVRLTPLEFGNLQKMDGPTEQCQDPLP---VPQKNCSARSG---ICEMILKGELFSGCAALVDISSYVEACRQDVCLCESLDPSDC--------ICHTLAEYSRQCAHAGGQPQDWR-GPNLCSQ-TCPLNMQHQECGSPCVDTCSNPQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNG--TLYAPGTNYSTDCTNTCSG--GQWSC-QDIPCAGTCSVMGGSHMSTFDGRQYTVHGDCTYVLSKPCDSNAF---------TVLVELRKCGLTESETCLKTVTLNLGGGQTEIMVK--ATGEVFVNQIYTQLPVS-TANATFF--RPSTFFIVGETNLGLQLEIQLSPIMQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNI--FQDPCSLSVENEKYAQHWCSLLTNASGPFSQCHATVNPSTFFSNCMYDTCNCEKSED-----CMCAALSSYVRACAAKGVLLSDWR-DGICTKPTITCP-KSMTYQYHISTCQPTCRALNEKDVTCHVSFIPVDGCTCPKGTFLDDLGK-CVQATSCPCYYKGSTVPNGESVQDSGAICTCTQGALTCIGGPAP-TPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCYSSEC-VPGCVCPNGLVADGNGGCVVTEDCPCVHNEATYRPGETIQVG---CNN-CTCENRMWQCT-DKPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLSDSKMEVVQKDVGQEPP-----YFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPDVLVPKDP---CTANPYRKSWAQKQCSIINSETFSACHAHVEPAKYYEACVNDACACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRG-----LEGCYPKCPPTAPIFDEGTMQCVSNCTVTFPCR----VNGKLYRPGASVPSDKNCDSCICTESGVRCTH 1265 HSP 2 Score: 201.83 bits (512), Expect = 3.710e-51 Identity = 147/503 (29.22%), Postives = 240/503 (47.71%), Query Frame = 2
Query: 1280 SATTICKQPQCF---NGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM-CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMK------YNQCGSRCTSTCR-----LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDC----KCEFHGRFYLP-YEIRQDR-CNNCTCTNGNWICTSN 2725
+ T +C P + + + G+ C K+C L M S+C+PGC CP DG CV E CPCV + E+TY+ G +++ C C C + WQC + + C C + GD HYITFD + + F+G CEYT + DN + S+ + +N C + T C ++I + +G + E+K++ K+ + + G + L ++++ G+ +LW+ + I ++LS +++ +V G+CGNF+ N ++D T D+ +F SWK +P+C ++ K C + +A K C + SE F CH+ K+++ C +D C C +G C C ++YA+ C + +SWRT ++CP C N + Y CG+ C TC+ LQ+ + E C C P FDE +CV S+C C +G+ Y P + D+ C++C CT CT N
Sbjct: 795 APTPVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCY-SSECVPGCVCPNGLVADGNGGCVVTEDCPCVHN--EATYRPGETIQVGCNNCTC-ENRMWQCTD-KPCLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQ---DNCGGNGSSQDAFRVITENIPC-GTTGTTCSKSIKIFLG------------NYELKLSDSKMEVVQKDVGQEPPYFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPD---VLVPKDPCTANPYRKSWAQKQC-SIINSETFSACHAHVEPAKYYEACVNDACAC---DSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQNPTGQCLQDLRGLEGCYPKCP-PTAPIFDEGTMQCV--SNCTVTFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHN 1266 HSP 3 Score: 114.775 bits (286), Expect = 9.533e-25 Identity = 113/427 (26.46%), Postives = 172/427 (40.28%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPP---------FMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCI---DIGKNVTDNTGCKKVCIEGCFCPDGLH-WQNNECVERKACE-CVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCA 4213
C Q+ QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N C S SC +SI Y R +N I+ N PG TS V + E G++V + + + +P + N G CG ++ R G I + WK VP +P + + CE++ S++FK CHDV+ +Y+ C+ D C D C+ +E Y + C + + WR TN C TC +V++ CG P C I ++T K EGCFCPD + N+ + +C+ C+ P G + +C++C C E C K K C + PE P E CP CA
Sbjct: 2031 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVFGYFRVLIDNYYCDVGDSVSCPQSIIVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNGIVTSRVGIKMYVTIQEIGVRVMF-SGLIFSVEVPFNLFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWK------VPNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYC---HMLDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCPDNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPKT--EGCFCPDSTTLFSTNDSICVPSCQWCLGP-RGEPVEPGHTISIDCQDCICKEATLTCQK---KACPQPTCPEPGFVPVPVALEAGQCCPQFSCA 2440 HSP 4 Score: 93.5893 bits (231), Expect = 2.419e-18 Identity = 118/492 (23.98%), Postives = 172/492 (34.96%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFV-----KISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALD-HCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC---------RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKE-----FCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVL----HNGECIKPFECP----CYMNNKMYSENDTI 2950
C ++ +C+ C GDPHYITFD ++ F +C Y V + I N C CP++I I+ +D+V + S + N + +S +Q G ++ +V + E GV ++++G +E+ + + G CG ++ D+ G + L WK P S +G + S + +L S FK CH +F+ C D C LD C YA CA + + + WR TN C CP +N Y CG C LQE+ K EGCFCP+ T F CV G P C +C C C C F P +A + G C C C C P CP+N+ L G C C C +N +Y D +
Sbjct: 2031 CCQHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-------PVFGYFRVLIDNYYCDVGDSVSCPQSI--IVEYHQDRVVLTRRPVSGVMTNQIIFNNKVVSPGFQQNG---IVTSRVGIKMYVTIQEIGVRVMFSGLIFSVEVPFN-LFANNTEGQCGTCTNDKKDECRLPGGSIASSCSEMSLHWKV-PNQPSCQGPPPTPTSVVPRPSPTPCPPSPLCELILSNTFKLCHDVIPPLQFYQGCLFDYCH-------MLDLEVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCP-DNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPK---TEGCFCPDSTTLFSTNDSICVPSCQWCLGPRGEPVEPGHTISIDCQDCICKEATLTCQKKACPQPTCPEPGFVPVPVA-LEAGQC---CPQFSCACNSSHCP-PPLHCPKNSSLIVTYEEGACCPTQNCSSQKGCEVNGTLYQPGDVV 2492
BLAST of von Willebrand factor vs. Ensembl Mouse
Match: Muc5b (mucin 5, subtype B, tracheobronchial [Source:MGI Symbol;Acc:MGI:1921430]) HSP 1 Score: 467.618 bits (1202), Expect = 1.047e-133 Identity = 354/1258 (28.14%), Postives = 556/1258 (44.20%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMK----ITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCY-STFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM---CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEE-FKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCPNENMKYNQCGSRCTSTCRLLQES----KCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFC-GNKL--GTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKV--IRECEECNCYEGAFRC 4102
CS H KTFDG+VF +P Y +F + A+Y+ F+ + + S + + Q +++ N LV ++ K+P++ G+++ ++KI L L++ + + + + CGLCG FNG+ + ++ N + + + D + ++ P + + +C+ + C + V + YLD C ++C C C +TF + Q ++A G +WR +C FN E+ EC S C C CE C+ GC+CP D C+P E+C C +G Y G +C +C C G W+C ++ C G C + G H TFD + + G C Y +L + ++ + C C + + + + + + I + + + F ++ L GN + I++ +++ ++ ++L + Y+ ++ G+CGNFN N+ DD GV E F +WKT +C + + Y+ C+ S ++A + C L ES F CH+ + F+ C D C C + C C A SSY CA + + LS +++C K CP + Y S C TCR L E+ ++GC CPEG TF CV + +C C FHG P E+ D C+CTNG C + C M + DC C K+C LD+ C QC + GC CP V NG CI +CPC N Y + I+R +C N C CK+ +W C NK C G C +G+ H+ +FDG+ + F+GSC Y +++D+C GN GTF++++EN+ CG++ ++C+K+IK + G+ +V + P G P Y + ++ GI V WD+ S+ + + + Y+ +VCGLCGNFD ND RS + D++ F +SWK SCP P C P R+ WA+ KC I+ S +F CH V+ YY+ CV D C CD G DC CFCTAV Y C + + L WRT + CP C G+ W + CG C +C +N T + +EGC+ CP + N + CV + C C D N ++ G +V C+ C C G +C
Sbjct: 81 CSTWGDFHYKTFDGDVFRFPGLCNY-----------VFSSHCGATYEDFNIQLRRGLEGSRPTVTYVLLRAQGLVIELSNGSVLVNGHREKLPYSRAGLLMEKSSGYVKISIRLVLTFLWNEEDSALLELDSKYINQTCGLCGDFNGLPA-----VSEFYTHNTRLTPVQFGNLQKLDGPTEQCQDVLPSAVSNCTDTED------ICRRTLLGPAFDKCTALVDVSMYLDACVQDLCRC----------PTCPCATFAEYSRQCAHA---GGYPQNWRHPD-LCNWTCPFN-MEYRECSSPCVDTCSNPQRSQLCEDHCMDGCFCPPGTVLDDVRHLGCLPLEQCHCT--HGGRIYAPGESFNTSCRSCTC-FGGLWKC-KDLPCPGTCSVQGGSHIFTFDEKLYNVHGDCSY---------ILTKKCADSSFTVLVDLRKCGVTDTENCLKAVTLNLNSGDTVIRIQVNGAVFLNSIFTQMPL---SAGNITIFKPSSFFIIVQTGMGLQLQVQLVPLMQVFVRLDRSYQGQMCGLCGNFNQNQADDFTALSGVVEGTGAAFSNTWKTQASCPNSKNT--YEDPCSYSVENENFAREWCSMLTESSGVFSACHATVSPVPFYSNCLFDTCNCENSED-----CMCAALSSYVFACAAKGVLLSGWRDKVCYKYTNNCP-QTKGYFYSVSSCQPTCRSLSEADVTCSVPFVTVDGCTCPEG-TFLNDKDHCVPVEECPCYFHGTVVAPREVVTDNGVVCSCTNGKLTCLGALMQKSTECQAPMVYLDCNNASVGHHGAECLKSCHTLDVECFSTQC-VSGCVCPSGLVADGNGGCIAEEDCPCIHNEVTYRPGE-IIR--VDCNN-CTCKNRRWECT-NKPCMGACVAYGDGHFVTFDGERYIFEGSCEYTLAQDYCRGNTSTNGTFRIVTENVPCGTTGTTCSKTIKIFVESYELILHEGNFKVVEQG---PSGDPP--YKIRFMGIFLVIEIRSGIVVSWDRKTSVFVRLQQHYKGRVCGLCGNFDDNAINDFTTRSQSVVGDVLEFGNSWKFSPSCPDAPVPKDPCIANPYRKSWAQKKCSIINSATFAACHSQVDSTKYYEACVHDVCACDSGGDCECFCTAVAAYAQACRDVGVCLSWRTPDICPLFCDYYNPHGECEWHYQPCGAPCLKTC----RNPTGHCLVDLPGLEGCYPQCPASQPFFNEDQMKCVAQ--CGCYDDNGNY-HDIGTQVPTAENCQSCLCTPGGLQC 1258 HSP 2 Score: 97.4413 bits (241), Expect = 1.890e-19 Identity = 92/373 (24.66%), Postives = 146/373 (39.14%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGI-QVKWDKAMSLEIMIPEKYQNKVCGLCGNFD-LRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKC--------EILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEY-EIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
+C TWG+ HY++FDG F F G C+YV S CG F NI ++ ++ L + SN V N + + + + ++ ++ + W++ S + + KY N+ CGLCG+F+ L ++ + + V F + K+ P C D P A + C L +F KC +V+V Y CV D C C C C +Y +C WR + C TC ++ C + C D+C N + C+ C++GCFCP G + C+ + C C Y G+ C C C+ G ++C L
Sbjct: 80 VCSTWGDFHYKTFDGDVFRFPGLCNYVFS-SHCGATYEDF-----NIQLRRGLEGSRPTVTYVLLRAQGLVIELSNGSVLVNGHREKLPYSRAGLLMEKSSGYVKISIRLVLTFLWNEEDSALLELDSKYINQTCGLCGDFNGLPAVSEFYTHNTRLTP--VQFGNLQKL-------DGPTEQCQ---DVLPSAVSNCTDTEDICRRTLLGPAFDKCTALVDVSMYLDACVQDLCRCP-----TCPCATFAEYSRQCAHAGGYPQNWRHPDLCNWTCPFNMEYRECSSPCVDTC----SNPQRSQLCEDHCMDGCFCPPGTVLDDVRHLGCLPLEQCHCTH--GGRIYAPGESFNTSCRSCTCFGGLWKCKDL 423 HSP 3 Score: 96.2857 bits (238), Expect = 4.605e-19 Identity = 98/397 (24.69%), Postives = 166/397 (41.81%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXX--XXCTKSIKFIYLNRVFQFVRGSNPIVSRN------------TNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKY----QNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKV----HSSC-----PVPQKPP-FMCSKRPDREPWAKAK-CEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAF 4096
C + +C C WG +HY +FDG + F +C+ V+ + + G +++ + CG+++ +SC +++ +Y N + + S ++ + + G T S T + + G + +D + +I +P +Y CG C N + +D R G I D + A W V SSC P+P P S P A CE++ S F +CH ++ + +++ CV+D C + D C ++E Y A C + WR T C +C + ++ CG + P SC ++ T + EGCFCP+ N+ CV C CV P K+ G+ I C+ C C G+
Sbjct: 4112 CHFHYECECFCSGWGHSHYLTFDGTSYTFLDNCTSVLMREI-HPRHGNLSILAHSYYCGATTNVTSCPRALS-VYYNSMEIILTISTTESGKDESLIIWDQMWIRSGFSKNGVTVSL--SGATTMSVNISTIGTIITFDGNI-FQIWLPYRYFSSNTEGQCGTCTNSQI---DDCRRPDGTIASDCQDMAKEWLVPGNSSSSCLAQPSPLPSTTPQTPVSSTLTSTPCPSAPLCELMLSQVFAECHRLIPPDAFFRSCVSDHCNANITD---MLCQSLEAYAALCRAQGVCTNWRNATGGLCDLSCPPTKEYRPCGPLHPASCNSRTQDSTTG-----MLAEGCFCPENQLLFNSRMDICVSE--CPCVGPDGLPKF-PGEHWISNCQACVCEPGSV 4489 HSP 4 Score: 83.9593 bits (206), Expect = 2.204e-15 Identity = 108/423 (25.53%), Postives = 170/423 (40.19%), Query Frame = 2
Query: 464 VIYGH-HIKTFDGNVFSYPLRGGYTLFMD-CLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW----------IDRDPSDLNVYRER-EQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICK------QPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCVPQEKCPCVRDNGESTYQQGYRLKL--NCETCICGMHGKWQCIENEEC-----EGRCFISGDPHYITFD 1639
V YG H TFDG + + YTL D C G + + + T CS ++KI V + +IL N F +V+ P + +G F+ I+ + + +DR +++V + ++CGLCG F+ +D+ +Q + +V F NSW + +DP N YR+ Q C+ ++ + C S+V KY + C ++C C + +C FC Y+ AC G+ + WR+ IC P + CG+ C K C + L GCY CP ++ + D CV Q C C DNG + + G ++ NC++C+C G QC+ N EGR + D Y T D
Sbjct: 900 VAYGDGHFVTFDGERYIFEGSCEYTLAQDYCRGNTSTNGTFRIVTENVPCGTTGTTCSKTIKIFVESYELILHEGN-FKVVEQG----PSGDPPYKIRFMGIFLVIEIRSGIVVSWDRKTSVFVRLQQHYKGRVCGLCGNFDDNAINDFTTRSQSVVGDVLEFGNSWKFSPSCPDAPVPKDPCIANPYRKSWAQKKCS-------------------IINSATFAACHSQVDSTKYYEACVHDVCAC-------DSGGDC-ECFCTAVAAYAQACRDVGVCLSWRTP-DICPLFCDYYNPHGECEWHYQPCGAPCLKTCRNPTGHCLVDLPGLEGCYPQCPASQPFFNEDQMKCVAQ--CGCYDDNG-NYHDIGTQVPTAENCQSCLC-TPGGLQCVYNLTACTCTYEGRTYNYNDVVYNTTD 1285 HSP 5 Score: 76.2554 bits (186), Expect = 4.674e-13 Identity = 121/492 (24.59%), Postives = 180/492 (36.59%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSE-TGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTK--VLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVT-GMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVE---YKGACALSRSYSDYANKVCH-----KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGE-TFDEAAKECVKISDCKC-EFHGRFYLPYEIRQDRCNNCTCTNGNWI--CTSNDCEGKEFCPK-------SMAW--FDCGICQKT-CTNLDLVCPKGQCQLPGCFCPENTVL--HNGECIKPFECP---CYMNNKMYSENDTIVRGP 2962
ECE C G HY+TFD + F +C T+V+ + N LSI + C + T CPR + V E + I+ S + + + + + +G +K V + + + T I+ G+ +I L +Y T G CG +++DD G D W P S + + + S + C +L S+ F +CH D FF C D C N + C++ +YA C + + +WR T LC CP +Y CG ++C + EGCFCPE + F+ CV S+C C G P E C C C G+ C CE ++ P+ ++ W D C +T C CPK LP C V N C + C C N+ +Y T GP
Sbjct: 4117 ECECFCSGWGHSHYLTFDGTSYTFLDNC--TSVLMREIHPRHGN-----LSILAHSYYCGATTNVTSCPRALSVYYNSMEIILTISTTESGKDESLIIWDQM-WIRSGFSKNGVTVSLSGATTMSVNISTIGTIITFDGNIFQIWLPYRYFSSNTEGQCGTCTNSQIDDCRRPDGTIASDCQDMAKEWLV-PGNSSSSCLAQPSPLPSTTPQTPVSSTLTSTPCPSAPLCELMLSQVFAECHRLIPPDAFFRSCVSDHC------NANITDMLCQSLEAYAALCRAQGVCTNWRNATGGLCDLSCP-PTKEYRPCGPLHPASCNSRTQDSTTGMLAEGCFCPENQLLFNSRMDICV--SECPCVGPDGLPKFPGEHWISNCQACVCEPGSVSVQCEPVKCESQDKPPQCTEAGFVTVTWPSADNPCCPETLCVCNSTTCPK---SLPTCEPGYELVQTHENSSCCPSYSCRPKLCTYNDTIYGVGTTFPGGP 4587 HSP 6 Score: 63.929 bits (154), Expect = 2.775e-9 Identity = 82/391 (20.97%), Postives = 140/391 (35.81%), Query Frame = 2
Query: 449 YYECSVI---YGH-HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILF------PKNEFALVKNNK-TKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIY-VYAPVTA-EKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQK----ALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNC------GQLGMELECESQCLPGCYCPRNR-YWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCIC 1549
+YEC +GH H TFDG +++ L ++ M + SY C C ++ + + IIL K+E ++ + + F+ G+ VS G+ I + + D NI+ ++ P G CG DD R + I + + W+ S + + P + + + LC+ M+ C + D + C + C N C+ E Y+ C +G+ +WR+AT C +E+ CG +C GM E GC+CP N+ ++ M + +CPCV +G + G NC+ C+C
Sbjct: 4115 HYECECFCSGWGHSHYLTFDGTSYTF-LDNCTSVLMREIHPRHGNLSILAHSYYCGATTNVTSCPRALSVYYNSMEIILTISTTESGKDESLIIWDQMWIRSGFSKNGVTVSLSGATTMSVNISTIGTIITFDGNIFQIWLPYRYFSSNTEGQCGTCTNSQIDDCRRPDGTIASDCQDMAKEWLVPGNSSSSCLAQPSPLPSTTPQTPVSSTLTSTPCPSAPLCELMLSQVFAE-CHRLIPPDAFFRSCVSDHCN-----------ANITDMLCQSLEAYAALCRAQGVCTNWRNATGGLCDLSCPPTKEYRPCGPLHPASCNSRTQDSTTGMLAE-------GCFCPENQLLFNSRMDICVSECPCVGPDGLPKF-PGEHWISNCQACVC 4484
BLAST of von Willebrand factor vs. UniProt/SwissProt
Match: sp|Q28295|VWF_CANLF (von Willebrand factor OS=Canis lupus familiaris OX=9615 GN=VWF PE=1 SV=2) HSP 1 Score: 504.212 bits (1297), Expect = 5.327e-145 Identity = 363/1282 (28.32%), Postives = 557/1282 (43.45%), Query Frame = 2
Query: 443 STYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKG-IDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKAL-TLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGV-IIGEDEDQVAINL-----LSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG---------------------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPF-----MCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKN----------CGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVI------RECEECNCYEGAFRC 4102
S+ CS+ G I TFD +++S+ Y L DC + F QN +S+ + +G I N L +P+ S G+ + + + K+ D + N V K CGLCG FN DD+R + + F NSW PCN ++D+ Q+ L CQ + ++ C V + ++ +C+ +C C+Q C + +Y+ AC +GI + + ++C+ P C G E+ EC S C + C L ++ C+ QC+ GC CP + D CV +C CV + Y G L +C TCIC + W C NEEC G C ++G H+ +FD R+F F G C Y D+ S+ I+ C D+ + C R++ V + G V + + +I++ ++ L QHT V RL +++ W+G R+ + LS Y K G+CGN+NGN DD +T G+ E ++ F +WK CE+ + +++ C+L+ + +A + C L S +F+ CH A + C +DVC+C R+ C C A ++YA CA ++++WR C CP + Y QCG+ C TCR L E C E C+EGCFCP G DE +CV + C C + G + P +I D C C +G CT++ G K CP + C KTC N DL C C + GC CP+ V H C+ CPC+ + Y+ +T+ +C N C+C+D KW C + C C G HY +FDG + F G C YV+ +D+CG+ GTF+++ N C S C K + + + G N P T F + + IL + + V WD +S+ + + YQ +VCGLCGNFD +ND + S +IE+D V+F +SWKV+ C +K P +C ++ + C IL SD F+ C+ +V+ E + C+ D C C+ DC CFC + Y C ++ + WRT CP C + + +N C CP +C + C C+EGC CP G L C++ + C + + + G K+I C+ C+C F C
Sbjct: 30 SSMARCSLFGGDFINTFDESMYSFAGDCSYLLAGDCQEHSVSLIGGF-----------QNGKRVSLSVYLGEFFDIHLFVNGTMLQGTQSISMPYASNGLYLEAEAGYYKLSSEAYGFVARIDGNGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFRTQEGTLTSDPYDFANSWALSSGEQRCKRVSPPSSPCNV----SSDEVQQVLWEQCQLLKSASVFARCHPLVDPEPFVALCERTLCTCVQGM----------ECPCAVLLEYARACAQQGIVLYGWTDHSVCR-PACPAGMEYKECVSPCTRTCQSLHVKEVCQEQCVDGCSCPEGQLLDEGHCVGSAECSCV--HAGQRYPPGASLLQDCHTCIC-RNSLWIC-SNEECPGECLVTGQSHFKSFDNRYFTFSGVCHYLLAQDCQDHTF---------SVVIETVQCADDLDAVCTRSVTVRLPGHHNSLVKLKHGGGVSMDGQDIQIPLLQGDLRIQHTVMASV----------RLSYGEDLQMDWDGRGRLLVTLSPAYAGKTCGLCGNYNGNRGDDFVTPAGLAEPLVEDFGNAWKLLGACENLQK--QHRDPCSLNPRQARFAEEAC-ALLTSSKFEPCHRAVGPQPYVQNCRYDVCSCSDGRD-----CLCSAVANYAAACARRGVHIAWREPGFCALSCP-QGQVYLQCGTPCNMTCRSLSYPEEDCNEVCLEGCFCPPGLYLDERG-DCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTTSGGLGSLLPNPVLSSPRSHRSKRSLSCRPPMVKLVCPADNPRAEGLECAKTCQNYDLQCMSTGC-VSGCLCPQGMVRHENRCVALERCPCFHQGQEYAPGETV---KIDC-NTCVCRDRKWNC-TDHVCDATCSAIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILVGNEGCSYPSVKCKKRVTILVEGGEIELFDGE-----VNVKKPMKDETHFE-VVESGQYVILLLGKALSVVWDHRLSISVTLKRTYQEQVCGLCGNFDGIQNNDFTSSSLQIEEDPVDFGNSWKVNPQCADTKKVPLDSSPAVCHNNIMKQTMVDSSCRILTSDIFQDCNRLVDPEPFLDICIYDTCSCESIGDCTCFCDTIAAYAHVCAQHGKVVAWRTATFCPQNCEERNLHENGYECEWRYNSCAPACPITC-----QHPEPLACPVQCVEGCHAHCPPGKILDELLQTCIDPEDCPVCE-VAGRRLAPGKKIILNPSDPEHCQICHCDGVNFTC 1234 HSP 2 Score: 122.479 bits (306), Expect = 3.341e-26 Identity = 89/368 (24.18%), Postives = 158/368 (42.93%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKF-IYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS------CPVPQKPPFMCSKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C +G +FD + F G CSY+++ D + + K + +YL F FV G+ +++ + P Y ++ + + G + D + ++++ ++Y NK CGLCGNF++ ++D R + G + D +FA+SW + S P P + S + W + C++LKS S F +CH +V+ E + C C C +G +C C + +Y C + I L W + C C +K C + C +C +++ C++ C++GC CP+G CV C CV +Y G ++++C C C + C+
Sbjct: 35 CSLFGGDFINTFDESMYSFAGDCSYLLAGDCQEHSVSLIGGFQNG-----------KRVSLSVYLGEFFDIHLFVNGTMLQGTQSISMPYAS-NGLYLEAEAGYYKLSSEAYGFVARIDGNGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFRTQEGTLTSDPYDFANSWALSSGEQRCKRVSPPSSPCNVSSDEVQQVLWEQ--CQLLKSASVFARCHPLVDPEPFVALCERTLCTCVQGMECPC--AVLLEYARACAQQGIVLYGWTDHSVCRPACPAGMEYKECVSPCTRTC----QSLHVKEVCQEQCVDGCSCPEGQLLDEGHCVGSAECSCVH--AGQRYPPGASLLQDCHTCICRNSLWICS 380 HSP 3 Score: 97.8265 bits (242), Expect = 1.015e-18 Identity = 112/459 (24.40%), Postives = 183/459 (39.87%), Query Frame = 2
Query: 1601 CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETF-------DEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ--KTCTNLDLVCPKGQ--CQLPGCFCP---------ENTVLHNGECIKPFECPC 2911
C S H +TFD ++F G C Y VL N L + + N C ++ C ++I V D +++ L S ++ VN +S+PY G+ +V + +R + + +++LS + + K G+CG + N +D + G D + W ++ + + E + C +S S C L SE F +CH F+ C D +C K+ CEA + YA C + + + WR C CP ++ YN C C C S C ++ EGCFCP + +EA +C+ + +F + ++ C CTC +G + CT + CP + A CG C+ + N + CP+ + C L C P + T+ + GEC F C C
Sbjct: 1950 CMGSSTRHIVTFDGQNFKLTGSCSY---------VLFQNKE-QDLEVILHNGACSPGAKETCMKSIEV----KHDGLSVELHSDMQMTVNGRLVSIPYV-GGDMEVNVYGTIMYEVRFNHLGHIFTFTPQNNEFQLQLSPRTFASKTYGLCGICDENGANDFILRDGTVTTDWKALIQEWTVQQLGKTCQPVPEEQ--CPVSSSSH------CQVLL-SELFAECHKVLAPATFYAMCQPD--SCHPKK-------VCEAIALYAHLCRTKGVCVDWRRANFCAMSCP-PSLVYNHCEHGCPRLCE-GNTSSCGDQPSEGCFCPPNQVMLEGSCVPEEACTQCISEDGVRHQFLETWVPAHQ----PCQICTCLSGRKVNCT------LQPCPTARAP-TCGPCEVARLRQNAEQCCPEYECVCDLVSCDLPPVPPCEDGLQMTLTNPGECRPNFTCAC 2362 HSP 4 Score: 95.9005 bits (237), Expect = 3.554e-18 Identity = 93/371 (25.07%), Postives = 145/371 (39.08%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGX-XXXXCTKSIKF------IYLNRVFQF-VRG---SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDRE--PWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV--DPITNLKYNTGDKVIRECEECNCYEG 4090
C +C H +FDG+ F+ GSCSYV+ + NK ++I N AC + +C KSI+ + L+ Q V G S P V + G + + H FT + + + + P + +K GLCG D ND R G + D W V Q+ C P+ + + + C++L S+ F +CH V+ +Y C D+C + C A+ Y C + + WR C +C V+ +C + CP C + + C EGCFCP CV +AC +C+ D + + T + C+ C C G
Sbjct: 1946 CPCVCMGSSTRHIVTFDGQNFKLTGSCSYVLFQ----NKEQDLEVILHNGACSPGAKETCMKSIEVKHDGLSVELHSDMQMTVNGRLVSIPYVGGDMEVNVYGTIMYEVRFNHLGHIFTFTPQNNEFQLQ-------LSPRTFASKTYGLCGICDENGANDFILRDGTVTTDWKALIQEWTV-------QQLGKTCQPVPEEQCPVSSSSHCQVLLSELFAECHKVLAPATFYAMCQPDSCHPKK------VCEAIALYAHLCRTKGVCVDWRRANFCAMSCPPSLVYNHCEHGCPRLC------EGNTSSCGDQPSEGCFCPPNQVMLEGSCVPEEACTQCISEDGVRHQFLETWVPAHQPCQICTCLSG 2286 HSP 5 Score: 56.6102 bits (135), Expect = 3.135e-6 Identity = 76/350 (21.71%), Postives = 130/350 (37.14%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVY-----------APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCP-CVRDNG 1492
HI TFDG F Y LF + L + N + ++T C S+++ ++ L + V IP+ + V+ G+ + E+ F+ +I+ + +P T K GLCG + ++D+ + + + K + W + EQ P ++ + CQ ++ + L C + + MCQ + C P+ C+ Y++ C KG+ VDWR A C C ++ C C + C G C Q GC+CP N+ CVP+E C C+ ++G
Sbjct: 1957 HIVTFDGQNFKLTGSCSYVLFQN--KEQDLEVILHNGACSPGAKET---CMKSIEVKHDGLSVELHSDMQMT-VNGRLVSIPYVGGDMEVNVYGTIM-------YEVRFNHLGHIFTFTPQNNEFQLQLSPRTFASKTYGLCGICDENGANDFILRDGTVTTDWKALIQEWTVQQLGKTCQPVPEEQCPVSSS------------SHCQVLL-SELFAECHKVLAPATFYAMCQPDSC-----HPKK---------VCEAIALYAHLCRTKGVCVDWRRAN-FCAM-SCPPSLVYNHCEHGCPRLCE--GNTSSCGDQPSEGCFCPPNQVMLEGSCVPEEACTQCISEDG 2262
BLAST of von Willebrand factor vs. UniProt/SwissProt
Match: sp|Q8CIZ8|VWF_MOUSE (von Willebrand factor OS=Mus musculus OX=10090 GN=Vwf PE=1 SV=2) HSP 1 Score: 504.212 bits (1297), Expect = 5.376e-145 Identity = 355/1264 (28.09%), Postives = 547/1264 (43.28%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKG-IDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG---------------------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQK-----PPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKN----------CGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVI------RECEECNC 4081
CS+ I TFD ++S+ Y L DC + F + N Q+ +S+ + +G I N + + +P+ S+G+ + + K+ D + N V K CGLCG FN DD+R + + F NSW S +R P + + D +Q CQ + ++ C V + ++ +C+ +C C T PE + C + +Y+ C +G+ + + + C+ P C G E+ EC S C + C L + C+ QC+ GC CP D CV CPCV + Y G L +C TCIC + W C NEEC G C ++G H+ +FD R+F F G C+Y D+ SI I+ C D+ + C R++ V + + + + L + ++ + LP+ G+ + I +RL +++ W+G R+ +KLS Y K G+CGN+NGN+ DD +T G+ E + F +WK C R ++ C+L+ + +A + C L S +F+ CH A + + C +DVC+C R+ C C A ++YA +CA + +++ WR C CP + Y QCG+ C TCR L + +C E C+EGC+CP G DE +CV + C C + G + P +I D C C +G CT++ G K CP C KTC N DL C + GC CP V H +C+ CPC+ Y+ DT+ G N C+C++ KW C N C G HY +FDG + F G C YV+ D+CG+ GTF+++ N C S C K + + + G + P + + + + IL + + V WD +S+ +++ YQ +VCGLCGNFD +ND S ++E+D VNF +SWKV S C +K P C ++ + C IL SD F+ C+ +V+ E Y C+ D C C+ DC CFC + Y C ++ + WRT CP +C K V +N C CP +C + C C+EGC CP G L CV+ + C + + + G K+ C+ C+C
Sbjct: 34 RCSLFGDDFINTFDETMYSFAGGCSYLLAGDCQKRS--FSILGN---------FQDGKRMSLSVYLGEFFDIHLFANGTVMQGDQSISMPYASQGLYLELEAGYYKLSSETFGFAARIDGNGNFQVLMSDRHFNKTCGLCGDFNIFAEDDFRTQEGTLTSDPYDFANSWAL---SSEEQRCKRASPPSRNCESSSGDMHQAMWEQCQLLKTASVFARCHPLVDPESFVALCEKILCTC-ATGPECA---------CPVLLEYARTCAQEGMVLYGWTDHSACR-PACPAGMEYKECVSPCPRTCQSLSINEVCQQQCVDGCSCPEGELLDEDRCVQSSDCPCV--HAGKRYPPGTSLSQDCNTCIC-RNSLWIC-SNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCEDHT---------FSIVIETMQCADDPDAVCTRSVSVRLSALHNSL-VKLKHGGAVGIDGQDVQLPFLQ-GDLR--IQHTVMASVRLSYAEDLQMDWDGRGRLLVKLSPVYSGKTCGLCGNYNGNKGDDFLTPAGLVEPLVVDFGNAWKLQGDCSDLRR--QHSDPCSLNPRLTRFAEEAC-ALLTSSKFEACHHAVSPLPYLQNCRYDVCSCSDSRD-----CLCNAVANYAAECARKGVHIGWREPGFCALGCP-QGQVYLQCGNSCNLTCRSLSLPDEECSEVCLEGCYCPPGLYQDERG-DCVPKAQCPCYYDGELFQPADIFSDHHTMCYCEDGFMHCTTSGTLGSLLPDTVLSSPLSHRSKRSLSCRPPMVKLVCPADNPRAQGLECAKTCQNYDLERMSLGC-VSGCLCPPGMVRHENKCVALERCPCFHQGAEYAPGDTVKIG----CNTCVCRERKWNC-TNHVCDATRSAIGMAHYLTFDGLKYLFPGECQYVLVYDYCGSNPGTFQILVGNEGCSYPSVKCRKRVTILVDGGELELFDGEVNVKR------PLRDESHFEVVESGRYVILLLGQALSVVWDHHLSISVVLKHTYQEQVCGLCGNFDGIQNNDSTTSSLQVEEDPVNFGNSWKVSSQCADTRKLSLDVSPATCHNNIMKQTMVDSACRILTSDVFQGCNRLVDPEPYLDICIYDTCSCESIGDCACFCDTIAAYAHVCAQHGQVVAWRTPTLCPQSCEEKNVRENGYECEWRYNSCAPACPVTC-----QHPEPLACPVQCVEGCHAHCPPGRILDELLQTCVDPQDCPVCE-VAGRRLAPGKKITLSPDDPAHCQNCHC 1227 HSP 2 Score: 125.176 bits (313), Expect = 4.509e-27 Identity = 90/372 (24.19%), Postives = 165/372 (44.35%), Query Frame = 2
Query: 3014 QNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNY--PPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMC-SKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
+++ + C +G+ +FD + F G CSY+++ D C + +F ++ N G + +YL F +N V + P Y + + + G + D + ++++ +++ NK CGLCG+F++ ++D R + G + D +FA+SW + S C P C S D +C++LK+ S F +CH +V+ E + C C C G +C C + +Y C + + L W + C C +K C + CP +C ++++ N C++ C++GC CP+G + CV+ C CV +Y G + ++C C C + C+
Sbjct: 27 RDRPSTARCSLFGDDFINTFDETMYSFAGGCSYLLAGD-CQKR--SFSILG-NFQDG------KRMSLSVYLGEFFDIHLFANGTVMQGDQSISMPYASQGLYLELEAGYYKLSSETFGFAARIDGNGNFQVLMSDRHFNKTCGLCGDFNIFAEDDFRTQEGTLTSDPYDFANSWALSSEEQRCKRASPPSRNCESSSGDMHQAMWEQCQLLKTASVFARCHPLVDPESFVALCEKILCTCATGPECAC--PVLLEYARTCAQEGMVLYGWTDHSACRPACPAGMEYKECVSPCPRTC----QSLSINEVCQQQCVDGCSCPEGELLDEDRCVQSSDCPCVH--AGKRYPPGTSLSQDCNTCICRNSLWICS 380 HSP 3 Score: 105.916 bits (263), Expect = 3.843e-21 Identity = 99/392 (25.26%), Postives = 157/392 (40.05%), Query Frame = 2
Query: 2963 ANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGX-XXXXCTKSIKFIYLNRVFQF-------VRGS---NPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDRE-PWA-KAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV--DPITNLKYNTGDKVIRECEECNCYEG 4090
AN Q+P ++ C C +C H +FDG+ F+ GSCSYVI + NK +++ N AC + +C KSI+ + + V G P V N G + H + +T + + + + P+ + +K+ GLCG D ND R G + D W V Q+P + C P+ + P + + C++L S SF +CH V+ ++ C D+C +R C + Y C + + WRT + C +C V+ +C CP C + + C EGCFCP + CV +AC +CV D + + T + C+ C C G
Sbjct: 1928 ANSQSPVRVEE---TCGCRWTCPCVCTGSSTRHIVTFDGQNFKLTGSCSYVIFQ----NKEQDLEVLLHNGACSPGAKQACMKSIEIKHAGVSAELHSNMEMAVDGRLVLAPYVGENMEVSIYGAIMYEVRFTHLGHILTYTPQNNEFQLQ-------LSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTV-------QQPGYTCQAVPEEQCPVSDSSHCQVLLSASFAECHKVIAPATFHTICQQDSCHQER------VCEVIASYAHLCRTSGVCVDWRTTDFCAMSCPPSLVYNHCERGCPRHCDG------NTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDGVRHQFLETWVPDHQPCQICMCLSG 2286 HSP 4 Score: 92.4337 bits (228), Expect = 4.038e-17 Identity = 116/461 (25.16%), Postives = 176/461 (38.18%), Query Frame = 2
Query: 1601 CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETF-------DEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ----KTCTNL-----DLVCPKGQCQLP------GCFCPENTVLHNGECIKPFECPC 2911
C S H +TFD ++F G C Y + L + + N C ++ C ++I + V+ L S+ E+ V+ + PY N +V I +R + + +++LS K + K+ G+CG + N +D G D + V W + + + E + C +S S C L S F +CH F C D +C +R CE +SYA C + + WRT + C CP ++ YN C C C S C + EGCFCP+ + F +EA +CV + +F + ++ C C C +G I CT+ CP + A CG C+ K TNL + VC C LP G P T+ + GEC F C C
Sbjct: 1950 CTGSSTRHIVTFDGQNFKLTGSCSYVIFQNKEQD----------LEVLLHNGACSPGAKQACMKSIEI----KHAGVSAELHSNMEMAVDGRLVLAPYVGE-NMEVSIYGAIMYEVRFTHLGHILTYTPQNNEFQLQLSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTVQQPGYTCQAVPEEQ--CPVSDSSH------CQVLL-SASFAECHKVIAPATFHTICQQD--SCHQER-------VCEVIASYAHLCRTSGVCVDWRTTDFCAMSCP-PSLVYNHCERGCPRHCD-GNTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDGVRHQFLETWVPDHQ----PCQICMCLSGRKINCTAQP------CPTARAP-TCGPCEVARLKQSTNLCCPEYECVCDLFNCNLPPVPPCEGGLQP--TLTNPGECRPTFTCDC 2362 HSP 5 Score: 60.8474 bits (146), Expect = 1.655e-7 Identity = 76/350 (21.71%), Postives = 128/350 (36.57%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVY-----------APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCP-CVRDNG 1492
HI TFDG F Y +F + L + N + + C S++I + L E A V P+ E + VS G+ + E+ F +I Y +P T K+ GLCG + ++D+ + + + K V W + P Y C A+ + + CQ ++ + C + + +CQ + C C++ Y++ C G+ VDWR+ T C C ++ C C ++C G C GC+CP+++ + CVP+E C CV ++G
Sbjct: 1957 HIVTFDGQNFKLTGSCSYVIFQN--KEQDLEVLLHNGA---CSPGAKQACMKSIEIKHAGVSAELHSNMEMA-VDGRLVLAPYVGENMEVSIYGAIM-------YEVRFTHLGHILTYTPQNNEFQLQLSPKTFASKMHGLCGICDENGANDFTLRDGTVTTDWKRLVQEWTVQQPG----------YTCQAVPEEQCPVSDS--SHCQVLLSASFAE-CHKVIAPATFHTICQQDSCH--------------QERVCEVIASYAHLCRTSGVCVDWRT-TDFCAM-SCPPSLVYNHCERGCPRHCD--GNTSFCGDHPSEGCFCPQHQVFLEGSCVPEEACTQCVGEDG 2262
BLAST of von Willebrand factor vs. UniProt/SwissProt
Match: sp|P04275|VWF_HUMAN (von Willebrand factor OS=Homo sapiens OX=9606 GN=VWF PE=1 SV=4) HSP 1 Score: 499.59 bits (1285), Expect = 1.801e-143 Identity = 353/1264 (27.93%), Postives = 557/1264 (44.07%), Query Frame = 2
Query: 443 STYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKG-IDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG---------------------------KEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPF-----MCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNC------GNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVI------RECEECNC 4081
S+ CS+ + TFDG+++S+ GY ++ G + QN +S+ + +G I N + + +P+ S+G+ + + + K+ G D N V K CGLCG FN DD+ + + F NSW S + ER P ++ + + + + CQ + ++ C V + ++ +C+ +CEC C +Y+ C +G+ + + + C P C G E+ +C S C + C L + C+ +C+ GC CP + D +CV +CPCV Y G L +C TCIC + +W C NEEC G C ++G H+ +FD R+F F G C+Y D+ SI I+ C D+ + C R++ V + + + + L + + ++ + LP + I T +RL +++ W+G R+ +KLS Y K G+CGN+NGN+ DD +T G+ E ++ F +WK + C+ + ++ CAL+ + ++ + C L S F+ CH A + + C +DVC+C R C C A +SYA CA + ++WR C CP + Y QCG+ C TCR L + +C E C+EGCFCP G DE +CV + C C + G + P +I D C C +G CT + G K CP + C KTC N DL C C + GC CP V H C+ CPC+ K Y+ +T+ G N C+C+D KW C + C C T G HY +FDG + F G C YV+ +D+CG+ GTF+++ N C S C K + + + G N P T F + + IL + + V WD+ +S+ +++ + YQ KVCGLCGNFD +ND+ + + ++E+D V+F +SWKV S C +K P C ++ + C IL SD F+ C+ +V+ E Y C+ D C C+ DC CFC + Y C ++ + WRT CP +C + + +N N C +C + + C C+EGC CP G L CV+ + C + + ++ +G KV C+ C+C
Sbjct: 30 SSTARCSLFGSDFVNTFDGSMYSF---AGYCSYLLAGGCQKRSFSIIG--------DFQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYASKGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWAL---SSGEQWCERASPPSSSCNISSGEMQKGLWEQCQLLKSTSVFARCHPLVDPEPFVALCEKTLCEC----------AGGLECACPALLEYARTCAQEGMVLYGWTDHSACS-PVCPAGMEYRQCVSPCARTCQSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVHSG--KRYPPGTSLSRDCNTCIC-RNSQWIC-SNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCQDHSF---------SIVIETVQCADDRDAVCTRSVTVRLPGLHNSL-VKLKHGAGVAMDGQDVQLPLL---KGDLRIQHTVTASVRLSYGEDLQMDWDGRGRLLVKLSPVYAGKTCGLCGNYNGNQGDDFLTPSGLAEPRVEDFGNAWKLHGDCQDLQK--QHSDPCALNPRMTRFSEEACAVL-TSPTFEACHRAVSPLPYLRNCRYDVCSCSDGRE-----CLCGALASYAAACAGRGVRVAWREPGRCELNCPKGQV-YLQCGTPCNLTCRSLSYPDEECNEACLEGCFCPPGLYMDERG-DCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTMSGVPGSLLPDAVLSSPLSHRSKRSLSCRPPMVKLVCPADNLRAEGLECTKTCQNYDLECMSMGC-VSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGETVKIG----CNTCVCQDRKWNC-TDHVCDATCSTIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILVGNKGCSHPSVKCKKRVTILVEGGEIELFDGE-----VNVKRPMKDETHFE-VVESGRYIILLLGKALSVVWDRHLSISVVLKQTYQEKVCGLCGNFDGIQNNDLTSSNLQVEEDPVDFGNSWKVSSQCADTRKVPLDSSPATCHNNIMKQTMVDSSCRILTSDVFQDCNKLVDPEPYLDVCIYDTCSCESIGDCACFCDTIAAYAHVCAQHGKVVTWRTATLCPQSCEERNLRENGYECEWRYNSCAPAC-QVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQTCVDPEDCPVCE-VAGRRFASGKKVTLNPSDPEHCQICHC 1227 HSP 2 Score: 130.568 bits (327), Expect = 1.111e-28 Identity = 97/386 (25.13%), Postives = 171/386 (44.30%), Query Frame = 2
Query: 2984 ICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKF-IYLNRVFQ---FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---CPVPQKPPFMC---SKRPDREPWAKAKCEILKSDS-FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
+C +G + + + C +G +FDG + F G CSY+++ G + +F +I + K + +YL F FV G+ + + P Y ++ + + G + D + + ++++ ++Y NK CGLCGNF++ ++D + G + D +FA+SW + S C P C S + W + C++LKS S F +CH +V+ E + C CEC G +C C A+ +Y C + + L W + C C ++ C + C +C +++ N C++ C++GC CP+G CVE C CV + +Y G + R+C C C + C+
Sbjct: 21 LCAEGT----RGRSSTARCSLFGSDFVNTFDGSMYSFAGYCSYLLAG---GCQKRSFSIIGD--------FQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYAS-KGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWALSSGEQWCERASPPSSSCNISSGEMQKGLWEQ--CQLLKSTSVFARCHPLVDPEPFVALCEKTLCECAGGLECAC--PALLEYARTCAQEGMVLYGWTDHSACSPVCPAGMEYRQCVSPCARTC----QSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVH--SGKRYPPGTSLSRDCNTCICRNSQWICS 380 HSP 3 Score: 101.679 bits (252), Expect = 7.298e-20 Identity = 118/461 (25.60%), Postives = 178/461 (38.61%), Query Frame = 2
Query: 1601 CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE--CEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETF-------DEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ--KTCTNLDLVCPKGQC----------QLPGCFCPENTVLHN-GECIKPFECPC 2911
C S H +TFD ++F G C Y VL N L + + N C + GC ++I V +++ L S E+ VN +S+PY GN +V + +R + + +++LS K + K G+CG + N +D M G D V W ++ + I+E + C + S C L F +CH F+ C D C H E CE +SYA C + + WRT + C CP ++ YN C C C S C + EGCFCP + +EA +C+ + +F + ++ C CTC +G + CT+ CP + A CG+C+ + N D CP+ +C +P C L N GEC F C C
Sbjct: 1950 CTGSSTRHIVTFDGQNFKLTGSCSY---------VLFQNKE-QDLEVILHNGACSPGARQGCMKSIEV----KHSALSVELHSDMEVTVNGRLVSVPYV-GGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQ--CLVPDSSH------CQVLL-LPLFAECHKVLAPATFYAICQQDSC-----------HQEQVCEVIASYAHLCRTNGVCVDWRTPDFCAMSCP-PSLVYNHCEHGCPRHCD-GNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQ----PCQICTCLSGRKVNCTTQP------CPTAKAP-TCGLCEVARLRQNADQCCPEYECVCDPVSCDLPPVPHCERGLQPTLTNPGECRPNFTCAC 2362 HSP 4 Score: 90.8929 bits (224), Expect = 1.175e-16 Identity = 92/371 (24.80%), Postives = 141/371 (38.01%), Query Frame = 2
Query: 3026 CSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXX-CTKSIKFIYLNRVFQF-------VRG---SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWA--KAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECV--DPITNLKYNTGDKVIRECEECNCYEG 4090
C +C H +FDG+ F+ GSCSYV+ + NK ++I N AC + C KSI+ + + V G S P V N G + H FT + + + + P+ + +K GLCG D ND R G + D W V Q+P C + + + C++L F +CH V+ +Y C D+C ++ C + Y C + + WRT + C +C V+ +C + CP C NV+ C EGCFCP CV +AC +C+ D + + + C+ C C G
Sbjct: 1946 CPCVCTGSSTRHIVTFDGQNFKLTGSCSYVLFQ----NKEQDLEVILHNGACSPGARQGCMKSIEVKHSALSVELHSDMEVTVNGRLVSVPYVGGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQ-------LSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTV-------QRPGQTCQPILEEQCLVPDSSHCQVLLLPLFAECHKVLAPATFYAICQQDSCHQEQ------VCEVIASYAHLCRTNGVCVDWRTPDFCAMSCPPSLVYNHCEHGCPRHC---DGNVSS---CGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQPCQICTCLSG 2286 HSP 5 Score: 56.225 bits (134), Expect = 3.868e-6 Identity = 73/351 (20.80%), Postives = 126/351 (35.90%), Query Frame = 2
Query: 476 HHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVY-----------APVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCP-CVRDNG 1492
HI TFDG F Y LF + L + N + + C S+++ ++ L E V +P+ + V+ G+ + E+ F+ +I+ + +P T K GLCG + ++D+ + + + KT V W + P EQ L + + + L L L C + + +CQ + C Q C++ Y++ C G+ VDWR+ C C ++ C C ++C G C GC+CP ++ CVP+E C C+ ++G
Sbjct: 1956 RHIVTFDGQNFKLTGSCSYVLFQN--KEQDLEVILHNGA---CSPGARQGCMKSIEVKHSALSVELHSDMEVT-VNGRLVSVPYVGGNMEVNVYGAIMH-------EVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQC------LVPDSSHCQVLLL-------PLFAECHKVLAPATFYAICQQDSCHQEQV--------------CEVIASYAHLCRTNGVCVDWRTP-DFCAM-SCPPSLVYNHCEHGCPRHCD--GNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDG 2262
BLAST of von Willebrand factor vs. UniProt/SwissProt
Match: sp|P98088|MUC5A_HUMAN (Mucin-5AC OS=Homo sapiens OX=9606 GN=MUC5AC PE=1 SV=4) HSP 1 Score: 490.73 bits (1262), Expect = 2.667e-140 Identity = 367/1280 (28.67%), Postives = 565/1280 (44.14%), Query Frame = 2
Query: 416 VTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISM-KITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT----FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEI--KLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKK---CPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT---FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCIDI-GKNVTDNTGCKKVCIEGCF--CPDG---LHWQNNECVERKACECVDP---ITNLKYNTGDKVI--RECEECNCYEGAFRCT 4105
V R +N ++ CS H KTFDG+VF +P Y +F + GAA Y FN +++Q + ++ ++ + +++ LV + +PF+ G+++ S+ K++ L ++++ D ++ + K CGLCG FNG+ ++++ N K F N DP+D C + +C++++ L C + V + YL+ C+ ++C C E++ + +C C +YS CT G+ DWR C Q +C N ++ EC S C C CE C+ GC+CP D T CVP KC CV + + Y G +C C C G+W C E C G C + G H+ TFD + + G C Y VL + ++ + C C ++ V + D Q + + +S E+ +N + LP V I + ST + ++ G+++ +++ +L+ K R + G+CGNFN + DD T GV E F ++KT C + R ++ C+LS YA C +L +++ F +CH+A ++ C D C C + C C A SSY CA + + L + +C K CP ++M Y+ S C TCR L E ++GC CP+G D+ K CV+ S+C C G E D CTCT+G C C M +FDC CQK+C LD+ C QC +PGC CP+ V G CI +CPC N Y TI G C N C C W C + C C +G+ HY +FDG+ + F G C Y + ++ CG K T F++++EN+ CG++ ++C+K+IK + G ++ + + Y + + ++ T G+ + WDK S+ I + +++ +VCGLCGNFD ND RS + D++ F +SWK+ SCP P C+ P R+ WA+ +C IL +F CH V YY+ CV DAC CD G DC CFCTAV Y C+E + + WRT CP C G+ W + CG C +C + G + D G +EGC+ CP +CV + P + Y G V + C+ C C E CT
Sbjct: 68 VVRASNPAHNGRV-CSTWGSFHYKTFDGDVFRFPGLCNY-VFSEHCGAA---YEDFNIQL----RRSQESAAPTLSRVLMKVDGVVIQLTKGSVLVNGHPVLLPFSQSGVLIQQSSSYTKVEARLGLVLMWNHDDSLLLELDTKYANKTCGLCGDFNGMPV-----VSELLSHNTKLTPMEFGNLQKMDDPTDQ----------CQDPVPEPPRNCSTGFGICEELLHGQLFSGCVALVDVGSYLEACRQDLCFC-----EDTDLLSC---VCHTLAEYSRQCTHAGGLPQDWR-GPDFCPQ-KCPNNMQYHECRSPCADTCSNQEHSRACEDHCVAGCFCPEGTVLDDIGQTGCVPVSKCACVYNG--AAYAPGATYSTDCTNCTCS-GGRWSCQE-VPCPGTCSVLGGAHFSTFDGKQYTVHGDCSY---------VLTKPCDSSAFTVLAELRRCGLTDSETCLKS--VTLSLDGAQTVVVIKASGEVFLNQIYTQLPIS---AANVTIFRPSTFFIIAQTSLGLQLNLQLVPTMQLFMQLAPKLRGQTCGLCGNFNSIQADDFRTLSGVVEATAAAFFNTFKTQAACPNIRN--SFEDPCSLSVENEKYAQHWCSQLTDADGPFGRCHAAVKPGTYYSNCMFDTCNCERSED-----CLCAALSSYVHACAAKGVQLGGWRDGVCTKPMTTCP-KSMTYHYHVSTCQPTCRSLSEGDITCSVGFIPVDGCICPKGTFLDDTGK-CVQASNCPCYHRGSMIPNGESVHDSGAICTCTHGKLSCIGGQAPAP-VCAAPMVFFDCRNATPGDTGAGCQKSCHTLDMTCYSPQC-VPGCVCPDGLVADGEGGCITAEDCPCVHNEASYRAGQTIRVG---C-NTCTCDSRMWRCTDDP-CLATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQ-----EVPYTIRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACACDSGGDCECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRG-----LEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECT 1269 HSP 2 Score: 106.686 bits (265), Expect = 1.967e-21 Identity = 101/413 (24.46%), Postives = 153/413 (37.05%), Query Frame = 2
Query: 3008 CEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKFIYLNRVFQFVRG------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME-GIQVKWDKAMSLEIMIP-EKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVH---------------------------------SSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C + QC +C WG+ HY +FDG Y+ F +C+YV+ + G F+++ +N CG+ SC +SI Y R +N I+ N PG S + E G+QV + + + +P K+ N G CG ++ R G + + W V S+ P PP C P C+++ S F+ CH V+ +Y+ CV D C D C+++E Y A C ++I + WR T CP TC +V++ CG P C EGCFCP+G+ + CV C+ P G V +C+EC C + T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQIV-PVYGHFRVLVDNYFCGAEDGLSCPRSIILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNGIVVSRIGVKMYATIPELGVQVMFS-GLIFSVEVPFSKFANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSP--------ICQLILSKVFEPCHTVIPPLLFYEGCVFDRCHMT---DLDVVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKVYQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGP-HGEPVKVGHTVGMDCQECTCEAATWTLT 5309 HSP 3 Score: 91.2781 bits (225), Expect = 1.093e-16 Identity = 103/425 (24.24%), Postives = 166/425 (39.06%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL----SITIQNTNCQDNSETGCPRTIGVIIGEDEDQV-----AINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN----PTCESDRGIVEYK---------------GACALSRSYSD---YANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC-----RLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKC-EFHGR-FYLPYEIRQDRCNNCTCTNGNWICT 2719
C + +C+ C GDPHYITFD ++ F +C Y V I P+ + + N C CPR+I I+ +D+V ++ + ++EI N +S ++ G +++ ++ + E GV+++++G +E+ SK + G CG + D+ T +G + W + P C G+ + + + +C +L S+ F+ CH+ F++ C D C LD C + YA CA +I + WR T +CP CP + + Y CG S C L EGCFCPEG T F +A+ CV +C HG + + + D C CTC W T
Sbjct: 4911 CCHHYQCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLVQQ-----------IVPVYGHFRVLVDNYFCGAEDGLSCPRSI--ILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNG---IVVSRIGVKMYATIPELGVQVMFSGLIFSVEVPFSK-FANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVGSTTVGPTTPPAPCLPSPIC-QLILSKVFEPCHTVIPPLLFYEGCVFDRC-----HMTDLD-VVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKV-YQPCGPSNPSYCYGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGPHGEPVKVGHTVGMD-CQECTCEAATWTLT 5309 HSP 4 Score: 76.6406 bits (187), Expect = 3.117e-12 Identity = 98/423 (23.17%), Postives = 171/423 (40.43%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQN--------DCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQ-DMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICK------QPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCV---PQEKCPCVRDNGESTYQQGYRLKL--NCETCICGMHGKWQCIENEEC----EGRCFISGDPHYITFD 1639
C+V H TFDG +S+ YTL + G +++ D F T+N CS ++KI +G + L + + ++P+ + +G ++ + L +L+D+ +I++ + ++CGLCG F+ I +D+ ++ + +V F NSW PS + ++ PC A + ++K+ Q ++ C + V+ +Y + C ++ C C + +C FC Y+ AC G+ V WR+ + IC P+ + CG C + C + + + L GCY CP D CV P P +Y+ G + NC++C+C G + E C G+ F GD Y T D
Sbjct: 903 CAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGK-------DSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQEVPYT-----IRQMGIYLVVDTDIGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSW-KLSPSCPDALAPKD--PCTA------NPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACAC-------DSGGDC-ECFCTAVAAYAQACHEVGLCVSWRTPS-ICPLFCDYYNPEGQCEWHYQPCGVPCLRTCRNPRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVCTYNGQRFHPGDVIYHTTD 1295
BLAST of von Willebrand factor vs. UniProt/SwissProt
Match: sp|Q9HC84|MUC5B_HUMAN (Mucin-5B OS=Homo sapiens OX=9606 GN=MUC5B PE=1 SV=3) HSP 1 Score: 473.781 bits (1218), Expect = 8.826e-135 Identity = 353/1252 (28.19%), Postives = 544/1252 (43.45%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDG---TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYE-SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCPNENMKYNQCGSRCTSTCRLLQESKCKEEC----IEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC---------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKL--GTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCT-----GKRVW--KNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKV--IRECEECNCYEGAFRC 4102
CS H KTFDG+VF +P Y C A Y FN + + ++ + Q ++L N L+ + ++P++ G++V G +IK+ L L++ + + + + CGLCG FNG+ + FN+ N + + + D + + P A + + +C + C + V YL C ++C C T P +TF + Q ++A G +WR +C + N Q ECGS C C CE C+ GC+CP D + C+P +CPC +G TY G C +C C G WQC ++ C G C + G H T+D + + G C Y +D+ + + +T N NC V + D AI + + + +N + LP N + +++ + + ++ ++L ++ ++ G+CGNFN N+ DD GV E F +WK C + R ++ C+LS +YA C +L + + F +CHS N F C D C C + C C A SSY CA + + LS + +C K CP ++ +Y C TCR L E+ ++GC CP G TF A CV +C C HG P E+ D C+CT G C + C M + DC C ++C LD+ C C + GC CP V +G CI +CPC N Y +TI +C N C C++ +W C ++ C G C +G+ H+ +FDG + F+GSC Y++++D+CG+ GTF++++ENI CG++ ++C+K+IK + G+ V+R P G P + F++ G+ V WD+ S+ I + + Y+ +VCGLCGNFD ND RS + D + F +SWK+ SCP P C+ P R+ WA+ +C IL +F C V+ YY+ CV DAC CD G DC CFCTAV Y C++ + + WRT + CP C G W + CG C +C +N + + +EGC+ CP + N + CV + C C D N Y+ G +V C+ CNC +C
Sbjct: 77 CSTWGDFHYKTFDGDVFRFPGLCNYVFSEHCRAA----YEDFNVQ---LRRGLVGSRPVVTRVVIKAQGLVLEASNGSVLINGQREELPYSRTGLLVEQSGDYIKVSIRLVLTFLWNGEDSALLELDPKYANQTCGLCGDFNGLPA-----FNEFYAHNARLTPLQFGNLQKLDGPTEQCPDPLPLPAGNCTDEE------GICHRTLLGPAFAECHALVDSTAYLAACAQDLCRC-PTCP--------CATFVEYSRQCAHA---GGQPRNWR-CPELCPRTCPLNMQH-QECGSPCTDTCSNPQRAQLCEDHCVDGCFCPPGTVLDDITHSGCLPLGQCPCT--HGGRTYSPGTSFNTTCSSCTCS-GGLWQC-QDLPCPGTCSVQGGAHISTYDEKLYDLHGDCSYVLSKKCADSSFTVLAELRKCGLT-DNENCLK----------AVTLSLDGGDTAIRVQADGGVFLNSIYTQLPLS-AANITLFTPSSFFIVVQTGLGLQLLVQLVPLMQVFVRLDPAHQGQMCGLCGNFNQNQADDFTALSGVVEATGAAFANTWKAQAACANARN--SFEDPCSLSVENENYARHWCSRLTDPNSAFSRCHSIINPKPFHSNCMFDTCNCERSED-----CLCAALSSYVHACAAKGVQLSDWRDGVCTKYMQNCP-KSQRYAYVVDACQPTCRGLSEADVTCSVSFVPVDGCTCPAG-TFLNDAGACVPAQECPCYAHGTVLAPGEVVHDEGAVCSCTGGKLSCLGASLQKSTGCAAPMVYLDCSNSSAGTPGAECLRSCHTLDVGCFSTHC-VSGCVCPPGLVSDGSGGCIAEEDCPCVHNEATYKPGETI---RVDC-NTCTCRNRRWEC-SHRLCLGTCVAYGDGHFITFDGDRYSFEGSCEYILAQDYCGDNTTHGTFRIVTENIPCGTTGTTCSKAIKLFVESYELILQEGTFKAVARG---PGGDPPYKIRYMGI---FLVIETHGMAVSWDRKTSVFIRLHQDYKGRVCGLCGNFDDNAINDFATRSRSVVGDALEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACRSQVDSTKYYEACVNDACACDSGGDCECFCTAVAAYAQACHDAGLCVSWRTPDTCPLFCDFYNPHGGCEWHYQPCGAPCLKTC----RNPSGHCLVDLPGLEGCYPKCPPSQPFFNEDQMKCVAQ--CGCYDKDGNY-YDVGARVPTAENCQSCNCTPSGIQC 1252 HSP 2 Score: 91.6633 bits (226), Expect = 7.952e-17 Identity = 98/398 (24.62%), Postives = 162/398 (40.70%), Query Frame = 2
Query: 2978 PCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXX----CTKSIKFIYLNR----VFQFVRGSNP--IVSRNTNYPPGGPTAFYWTSDVDTHFILFTME--GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS----CPVPQ-KPPFMCSKRPDREPWAKAK------CEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCP-DGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGA 4093
P D C+ + +C IC WG +HY +FDG + F+G+C+YV+ + + G L +N C +S+++ C +++ Y + V G I+ G S + T + + G+ V ++ + + + N G CG +D R G + A +W V S C P PP P C+++ S F +CH++V ++ C++D C RG V C ++E Y C + WR T C TC +V+K CG I P +C N + + + EGCFCP D + + + + +AC CV P K+ G++ + C+ C C EG+
Sbjct: 5055 PIKVSDPSQPCDFHYECECICSMWGGSHYSTFDGTSYTFRGNCTYVLMREIHA-RFGNLSLYLDNHYCTASATAAAARCPRALSIHYKSMDIVLTVTMVHGKEEGLILFDQIPVSSGFSKNGVLVSVLGTTTMRVDIPALGVSVTFNGQVFQARLPYSLFHNNTEGQCGTCTNNQRDDCLQRDGTTAASCKDMAKTWLVPDSRKDGCWAPTGTPPTASPAAPVSSTPTPTPCPPQPLCDLMLSQVFAECHNLVPPGPFFNACISDHC---RGRLEVP-CQSLEAYAELCRARGVCSDWRGATGGLCDLTCPPTKVYKPCGPIQPATC-----NSRNQSPQLEGMAEGCFCPEDQILFNAHMGICVQACPCVGPDGFPKF-PGERWVSNCQSCVCDEGS 5441 HSP 3 Score: 86.2705 bits (212), Expect = 3.448e-15 Identity = 126/548 (22.99%), Postives = 190/548 (34.67%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETG---CPRTIGVIIGEDEDQVAINLLSSSE---IKVNFVKISLPYQHTGNTKVLIDQVSTDILRLK-SETGVEILWNGG---SRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSW----KTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYE---SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGET-FDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWI--CTSNDCEGK---------------------EFCPKSMAWFDCGICQKTCTNLDLVCPKGQ----CQLPGCFCPE----------NTVLHNGECIKPFECPCYMNNKMYSE-NDTIVRGPANCQNPCICKDGKWVCEQNKQCSGIC 3040
C + ECE C + G HY TFD + F G+C Y + ++ LS+ + N C ++ CPR + + + + + ++ E I + + +S + G VL+ + T +R+ GV + +NG +R+ L + G CG N+ DD + G +W C + G A S L + S+ F +CH+ FF+ C D C G L+ C++ +YA C + WR T LC CP + Y CG +TC +S E EGCFCPE + F+ CV+ C G P E C +C C G+ C C+ + CP+++ C TC VCP GQ Q G CP N + P PC+M + + D V+ + N C G QC G C
Sbjct: 5065 CDFHYECECICSMWGGSHYSTFDGTSYTFRGNCTYVLMREI-------HARFGNLSLYLDNHYCTASATAAAARCPRALSIHYKSMDIVLTVTMVHGKEEGLILFDQIPVSSGFSKNG---VLVSVLGTTTMRVDIPALGVSVTFNGQVFQARLPYSL---FHNNTEGQCGTCTNNQRDDCLQRDGTTAASCKDMAKTWLVPDSRKDGCWAPTGTPPTASPAAPVSSTPTPTPCPPQPLCDLMLSQVFAECHNLVPPGPFFNACISDHC------RGRLE-VPCQSLEAYAELCRARGVCSDWRGATGGLCDLTCPPTKV-YKPCGPIQPATCNSRNQSPQLEGMAEGCFCPEDQILFNAHMGICVQACPCVGP-DGFPKFPGERWVSNCQSCVCDEGSVSVQCKPLPCDAQGQPPPCNRPGFVTVTRPRAENPCCPETV----CVCNTTTCPQSLPVCPPGQESICTQEEGDCCPTFRCRPQLCSYNGTFYGVGATFPGALPCHMCTCLSGDTQDPTVQCQEDACNNTTCPQGFEYKRVAGQCCGEC 5586
BLAST of von Willebrand factor vs. TrEMBL
Match: A0A267GUZ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026350g1 PE=4 SV=1) HSP 1 Score: 1118.61 bits (2892), Expect = 0.000e+0 Identity = 564/1360 (41.47%), Postives = 790/1360 (58.09%), Query Frame = 2
Query: 209 NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRE---REQYPCNALKLKNNDQYQKALTLCQDMVK-NNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLC--PNNFCARRCNGIIECRDGEDEDLC 4267
NG C GRC C+LG++G++C P+C C++ G C+APN+C C D Y G C + +S D + + AV VNNT Y CS G H TFDG F +P G Y L DC+ + + VYFN S +C D Q C +S+KI G + + P V +P + + VS+ + I ++G+DDL I +D DRNI VYAP T + +CGLCG F+G ++D+ D V F +W D P D NVY Q+PC L+ K+ + A +LC + ++ C S V Y MC ++C CL N+T+ C C YS ACT +GI ++WR + +C P+C NG+ FSEC +C + CG ++ +C + C+ GC+CP R+WDG CV ++ CPC R +Y G + CE C+C G W+C +EC G C +SGDP Y TFD RHF F+GHCEY +M + S P+++ ++N C ++ C + + + +G E Q I L S S +KV+ +SLP + + I VST +LR+++ G ++LWNG +RI+I+LSK +R KVTG+CGNFN + DD+++ G+D KDI +F SWKTNPTC++ V Y GACA S+ + YA VC+KLY FK CHS + +QC HDVC+CG +R G + CEC AF+SYAR CAL+ L+WR++ LCP KCP M+Y +CGS C TC+ ++ S C EEC+EGC CP G+ DE CV + CKC ++G Y ++R+D+CNNCTCT+GNW CT+ C CP M W +C CQ++C N+++ CPK C+ GC CP+ VLH G+C+ CPC M K+Y+EN + RG C N C+C+ G W C NK C C+ WG+ HYE+FDG+Y++F G+CSYV++ED+CG GTF++ ENI CG+ SCTKS+K Y + V VRG++PI+SRN +P P A Y + T +GI+V+WD M + + + + KVCGLCGNF+ + D+D+RAR+GE+ +D V FADSWKV ++C P P CS +R+ WA+ KC IL+S F CH V V +Y +CV D C C + DDC+C+CTAV QYV RCNE + +KWR ++CP C V+K CG+ CP CIDI K T + CK C+EGCFCPDG ++ C +R+ C CV + K G K+I +C+EC C G+ C + +C T P C +C +CA+RC+G EC G+DE C
Sbjct: 167 NGKCVRGRCVCNLGYKGDKCELPLCKNNCNSVGTCLAPNKCRCPDGYHGDLCQVSALYAS--DSTAFVAVRAVNNTRYRNSAVCSAFGGKHFLTFDGKYFDFPAHGSYVLLQDCVNSEAKYQVYFNGSRNCADA-AQTRCQVSLKIVAGPAKVEIMPGGSV-FVNEKPVTLPTIAGIVSVSNTANHITVRGLDDLAITYDGDRNINVYAPKTMSRTVCGLCGRFDGKAANDFTDLMNKPLTRVVDFAKAWRDESPDDKNVYPSVPADAQHPCARLRSKSTSLLESAQSLCSILRSGSSTFSACHSRVNSSVYESMCMKDMCTCLLNG--NATV--CNEQRCNSATLYSRACTQQGIVINWRQSN-LCPAPKCSNGKVFSECSHDCNQKCGTNTVDDDCGTHCVDGCFCPSGRFWDGHKCVIRDNCPCNRAGDSRSYPVGSKYSHKCEQCVCDRGGSWKCSPTDECYGYCTVSGDPTYRTFDGRHFSFEGHCEYIMMMPQRS---AAESLEYPVAVYVENKYCDSVTDVLCAKLVTLQMGSGEKQHTIRLESLSRVKVDSAYVSLPVKVH---DITIRLVSTSMLRVETNKGFDLLWNGRTRIQIRLSKAFRNKVTGLCGNFNNDMKDDTISPTGIDVKDIRQFAKSWKTNPTCDAKTRFV-YNGACATSKEFDSYATSVCNKLYTESVFKACHSRVDPKWALEQCKHDVCSCGSRRKGVMSQCECTAFASYARSCALDGKVLNWRSSSLCPVKCP-PTMEYQECGSNCGKTCKRVEASPCIEECVEGCQCPSGQALDEEKGTCVPRNQCKCVYNGVSYTNGQVRKDKCNNCTCTDGNWECTNLTCTDSNTCPAGMVWSECSDCQRSCENINIECPKSSCKSGGCVCPKGQVLHQGKCVDKGNCPCAMAGKVYAENSVVPRGSTRCYNYCMCRKGSWQCTNNK-CPSTCRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMSCTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPCSS--ERKRWAEKKCAILRSSEFTSCHSKVEVNEFYDKCVMDTCAC-KLDDCLCYCTAVSQYVLRCNEMGVKIKWRNPDDCPVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCV--VGGKKIAYGKKIIYKCQECTCTAGSLECKPYYLGDCATFPTTVPPTTTPFCTGPGLIICRGERKYCAKRCDGNRECMYGDDEQCC 1503 HSP 2 Score: 92.4337 bits (228), Expect = 1.260e-14 Identity = 105/467 (22.48%), Postives = 185/467 (39.61%), Query Frame = 2
Query: 200 DCVNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNY-----GAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLG----MELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC 1573
+C CK G C C G ++ GKCV C C N+++ + S + NY G+ NN ST C V H +TFDG + + Y L D G + + C + C+ S+KIT + L + + +N + + G ++ + D +E+ +D + V +K+CGLCG FN DD R + ++ F +SW R+ PC++ ++ + A C +++++ +C S+V+++++ D C + C C +++C +C QY C G+ + WR+ C + CGS C + C + + +C+S C+ GC+CP + + +C +E+C CV + Y G ++ C+ C C G +C
Sbjct: 1022 ECPKSSCKSGGCVCPKGQVLHQ-------------GKCVDKGNCPCAMAGKVYAENSVVPRGS-TRCYNYCMCRKGSWQCTNNKCPST---CRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMS--CTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRN--TCPKPPPTPIAPCSS------ERKRWAEKKCA-ILRSSEFTSCHSKVEVNEFYDKCVMDTCAC--------KLDDCL-CYCTAVSQYVLRCNEMGVKIKWRNPDDC--PVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCVVGGKKIAY--GKKIIYKCQECTC-TAGSLEC 1446 HSP 3 Score: 56.225 bits (134), Expect = 6.043e-8 Identity = 87/364 (23.90%), Postives = 162/364 (44.51%), Query Frame = 2
Query: 4322 DQNEIMKSGETKIVDQCIKYICDDGNIVTKTKFCNITCDEHSEILKQHVDSDKCCECVSIRTTTLMVSATTTRLVTCEMYTLTVEKNFNILYKLKSDSIGNSIENVI----YDSDKSWTV-VSNNAVLIVEFLPKQPXXXXXXXXXXXXXXXXXXXXXDIVEKDDTWKDADSYTLAKSSTKDGNLFSYTFTSATKDGIPGRFVRFSFILSNQITEVIVIRKLMIKICGSQKTTEIQPSSTSGMVSCPPKGTTIRQNECLITMCIDGEWITKMNCPISCLPGEIKVTYDDERCCQCVNANQTTISMPVISSTKLVSTKGPCKPPL-IHKCIDDCEGICHYLQENQCHNLLTSTNRICKDACVCPQ 5395
D++ + S + C Y C G +++ K CN+TC + + + +CCEC + TTT++V+ TTT L C + +T+ + +++++ S N+I+ + ++ SW+ S++ +V + +P +I ++ + A +V ++ D W Y+ ++ L S +F++ T DG+ VR +F + + + L+I C TT + P++T S G +EC+ +C G WI K +C I C V + + CC+C TT + ++ +T C P+ C+ C CHY+ E +C + C+ C CP
Sbjct: 1799 DKDGVKHSEGSTWRSGCAIYTCTKG-VISSRKECNVTCAPGFILRQYPTNPTRCCECERLATTTVIVTPTTTTLTKCTHHEITLASDSGSKFEMRASS--NNIDRLKALKGAPANSSWSPDASDDEPRLVFAITPRPGSRTIITKLELTATRARSVEVEFTSGSDIW-----YSKRVATPSSSGLVSLSFSNETTDGVSATAVRVTFPRGSGVKVNPSVSGLLISACFEVSTTVVAPTTTPICAS----GDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRK-CHYI-EPECS---SEGKFPCESGCQCPS 2145 HSP 4 Score: 33.4982 bits (75), Expect = 6.043e-8 Identity = 13/37 (35.14%), Postives = 19/37 (51.35%), Query Frame = 3
Query: 5418 CVDTRQCECVDNDGKIRKPNETWTDRCNQYRCVDNQV 5528
CV + C C DGK+R + W C +Y C +N +
Sbjct: 2155 CVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTI 2191 HSP 5 Score: 63.929 bits (154), Expect = 5.322e-6 Identity = 29/62 (46.77%), Postives = 37/62 (59.68%), Query Frame = 2
Query: 191 CSPDCVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTL 373
CSP C NG+C C+CS GW G C+ P+C C+NGG CVAPN C C + G C ++
Sbjct: 91 CSPKCDNGMCVAPNSCNCSSGWSGATCNKPVCAKECANGGICVAPNTCRCAAGFDGEQCQSV 152
BLAST of von Willebrand factor vs. TrEMBL
Match: A0A267DLR6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026350g2 PE=4 SV=1) HSP 1 Score: 1113.98 bits (2880), Expect = 0.000e+0 Identity = 564/1360 (41.47%), Postives = 791/1360 (58.16%), Query Frame = 2
Query: 209 NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRE---REQYPCNALKLKNNDQYQKALTLCQDMVK-NNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLC--PNNFCARRCNGIIECRDGEDEDLC 4267
NG C GRC C+LG++G++C P+C C++ G C+APN+C C D Y G C + +S D + + AV VNNT Y CS G H TFDG F +P G Y L DC+ + + VYFN S +C D Q C +S+KI G + + P V +P + + VS+ + I ++G+DDL I +D DRNI VYAP T + +CGLCG F+G ++D+ D +V F +W D P D NVY Q+PC L+ K+ + A +LC + ++ C S V Y MC ++C CL N+T+ C C YS ACT +GI ++WR + +C P+C NG+ FSEC +C + CG ++ +C + C+ GC+CP R+WDG CV ++ CPC R +Y G + CE C+C G W+C +EC G C +SGDP Y TFD RHF F+GHCEY +M + S P+++ ++N C ++ C + + + +G E Q I L S S +KV+ +SLP + + I VST +LR+++ G ++LWNG +RI+I+LSK +R KVTG+CGNFN + DD+++ G+D KDI +F SWKTNPTC++ V Y GACA S+ + YA VC+KLY FK CHS + +QC HDVC+CG +R G + CEC AF+SYAR CAL+ L+WR++ LCP KCP M+Y +CGS C TC+ ++ S C EEC+EGC CP G+ DE CV + CKC ++G Y ++R+D+CNNCTCT+GNW CT+ C CP M W +C CQ++C N+++ CPK C+ GC CP+ VLH G+C+ CPC M K+Y+EN + RG C N C+C+ G W C NK C C+ WG+ HYE+FDG+Y++F G+CSYV++ED+CG GTF++ ENI CG+ SCTKS+K Y + V VRG++PI+SRN +P P A Y + T +GI+V+WD M + + + + KVCGLCGNF+ + D+D+RAR+GE+ +D V FADSWKV ++C P P CS +R+ WA+ KC IL+S F CH V V +Y +CV D C C + DDC+C+CTAV QYV RCNE + +KWR ++CP C V+K CG+ CP CIDI K T + CK C+EGCFCPDG ++ C +R+ C CV + K G K+I +C+EC C G+ C + +C T P C +C +CA+RC+G EC G+DE C
Sbjct: 167 NGKCVRGRCVCNLGYKGDKCELPLCKNNCNSVGTCLAPNKCRCPDGYHGDLCQVSALYAS--DSTAFVAVRAVNNTRYRNSAVCSAFGGKHFLTFDGKYFDFPAHGSYVLLQDCVNSEAKYQVYFNGSRNCADA-AQTRCQVSLKIVAGPAKVEIMPGGSV-FVNEKPVTLPTIAGIVSVSNTANHITVRGLDDLAITYDGDRNINVYAPKTMSRTVCGLCGRFDGKAANDFTDLMNKPLTHVVDFAKAWRDESPDDKNVYPSVPADAQHPCARLRSKSTSLLESAQSLCSILRSGSSTFSACHSRVNSSVYESMCMKDMCTCLLNG--NATV--CNEQRCNSATLYSRACTQQGIVINWRQ-SNLCPAPKCSNGKVFSECSHDCNQKCGTNTVDDDCGTHCVDGCFCPSGRFWDGHKCVIRDNCPCNRAGDSRSYPVGSKYSHKCEQCVCDRGGSWKCSPTDECYGYCTVSGDPTYRTFDGRHFSFEGHCEYIMMMPQRS---AAESLEYPVAVYVENKYCDSVTDVLCAKLVTLQMGSGEKQHTIRLESLSRVKVDSAYVSLPVKVH---DITIRLVSTSMLRVETNKGFDLLWNGRTRIQIRLSKAFRNKVTGLCGNFNNDMKDDTISPTGIDVKDIRQFAKSWKTNPTCDAKTRFV-YNGACATSKEFDSYATSVCNKLYTESVFKACHSRVDPKWALEQCKHDVCSCGSRRKGVMSQCECTAFASYARSCALDGKVLNWRSSSLCPVKCP-PTMEYQECGSNCGKTCKRVEASPCIEECVEGCQCPSGQALDEEKGTCVPRNQCKCVYNGVSYTNGQVRKDKCNNCTCTDGNWECTNLTCTDSNTCPAGMVWSECSDCQRSCENINIECPKSSCKSGGCVCPKGQVLHQGKCVDKGNCPCAMAGKVYAENSVVPRGSTRCYNYCMCRKGSWQCTNNK-CPSTCRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMSCTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPCSS--ERKRWAEKKCAILRSSEFTSCHSKVEVNEFYDKCVMDTCAC-KLDDCLCYCTAVSQYVLRCNEMGVKIKWRNPDDCPVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCV--VGGKKIAYGKKIIYKCQECTCTAGSLECKPYYLGDCATFPTTVPPTTTPFCTGPGLIICRGERKYCAKRCDGNRECMYGDDEQCC 1503 HSP 2 Score: 95.9005 bits (237), Expect = 1.096e-15 Identity = 103/467 (22.06%), Postives = 183/467 (39.19%), Query Frame = 2
Query: 200 DCVNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNY-----GAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLG----MELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC 1573
+C CK G C C G ++ GKCV C C N+++ + S + NY G+ NN ST C V H +TFDG + + Y L D G + + C + C+ S+KIT + L + + +N + + G ++ + D +E+ +D + V +K+CGLCG FN DD R + ++ F +SW R+ ++++ + A C +++++ +C S+V+++++ D C + C C +++C +C QY C G+ + WR+ C + CGS C + C + + +C+S C+ GC+CP + + +C +E+C CV + Y G ++ C+ C C G +C
Sbjct: 1022 ECPKSSCKSGGCVCPKGQVLHQ-------------GKCVDKGNCPCAMAGKVYAENSVVPRGS-TRCYNYCMCRKGSWQCTNNKCPST---CRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMS--CTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPC--------SSERKRWAEKKCA-ILRSSEFTSCHSKVEVNEFYDKCVMDTCAC--------KLDDCL-CYCTAVSQYVLRCNEMGVKIKWRNPDDC--PVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCVVGGKKIAY--GKKIIYKCQECTC-TAGSLEC 1446 HSP 3 Score: 67.0106 bits (162), Expect = 4.362e-11 Identity = 87/364 (23.90%), Postives = 163/364 (44.78%), Query Frame = 2
Query: 4322 DQNEIMKSGETKIVDQCIKYICDDGNIVTKTKFCNITCDEHSEILKQHVDSDKCCECVSIRTTTLMVSATTTRLVTCEMYTLTVEKNFNILYKLKSDSIGNSIENVI----YDSDKSWTV-VSNNAVLIVEFLPKQPXXXXXXXXXXXXXXXXXXXXXDIVEKDDTWKDADSYTLAKSSTKDGNLFSYTFTSATKDGIPGRFVRFSFILSNQITEVIVIRKLMIKICGSQKTTEIQPSSTSGMVSCPPKGTTIRQNECLITMCIDGEWITKMNCPISCLPGEIKVTYDDERCCQCVNANQTTISMPVISSTKLVSTKGPCKPPL-IHKCIDDCEGICHYLQENQCHNLLTSTNRICKDACVCPQ 5395
D++ + S + C Y C G +++ K CN+TC + + + +CCEC + TTT++V+ TTT L+ C + +T+ + +++++ S N+I+ + ++ SW+ S++ +V + +P +I ++ + A +V ++ D W Y+ ++ L S +F++ T DG+ VR +F + + + L+I C TT + P++T S G +EC+ +C G WI K +C I C V + + CC+C TT + ++ +T C P+ C+ C CHY+ E +C + C+ C CP
Sbjct: 1799 DKDGVKHSEGSTWRSGCAIYTCSKG-VISSRKECNVTCAPGFILRQYPTNPTRCCECERLATTTVIVTPTTTTLMKCTHHEITLASDSGSKFEMRASS--NNIDRLKALKGAPANSSWSPDASDDEPRLVFAITPRPGSRTIITKLELTATRARSVEVEFTSGSDIW-----YSKRVATPSSSGLVSLSFSNETTDGVSATAVRVTFPRGSGVKVNPSVSGLLISACFEVSTTVVAPTTTPICAS----GDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRK-CHYI-EPECS---SEGKFPCESGCQCPS 2145 HSP 4 Score: 33.113 bits (74), Expect = 4.362e-11 Identity = 13/37 (35.14%), Postives = 19/37 (51.35%), Query Frame = 3
Query: 5418 CVDTRQCECVDNDGKIRKPNETWTDRCNQYRCVDNQV 5528
CV + C C DGK+R + W C +Y C +N +
Sbjct: 2155 CVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTI 2191 HSP 5 Score: 63.5438 bits (153), Expect = 6.517e-6 Identity = 32/76 (42.11%), Postives = 41/76 (53.95%), Query Frame = 2
Query: 191 CSPDCVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGA 415
CSP C NG+C C+CS GW G C+ P+C C+NGG CVAPN C C + G C ++ S V G+
Sbjct: 91 CSPKCDNGMCVAPNSCNCSSGWSGATCNKPVCAKECANGGICVAPNTCRCAAGFDGEQCQSVATTVSSVCVGGCGS 166
BLAST of von Willebrand factor vs. TrEMBL
Match: A0A1I8GY38 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 1090.49 bits (2819), Expect = 0.000e+0 Identity = 558/1360 (41.03%), Postives = 783/1360 (57.57%), Query Frame = 2
Query: 209 NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRE---REQYPCNALKLKNNDQYQKALTLCQDMVK-NNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLC--PNNFCARRCNGIIECRDGEDEDLC 4267
NG C GRC C+LG++G++C P+C C++ G C+APN+C C D Y G C + +S D + + AV VNNT Y CS G H TFDG F +P G Y L DC+ + + VYFN S +C D Q C +S+KI G + + P V +P + + VS+ + I ++G+DDL I +D DRNI VYAP T + +CGLCG F+G ++D+ D +V F +W D P D NVY Q+PC L+ K+ + A +LC + ++ C S V Y MC ++C CL N+T+ C C YS ACT +GI ++WR + +C P+C NG+ FSEC +C + CG ++ +C + C+ GC+CP R+WDG CV ++ CPC R +Y G + CE C+C G W+C +EC G C +SGDP Y TFD RHF F+GHCEY +M + S P+++ ++N C ++ C + + + +G E Q I L S S +KV+ +SLP + + I VST +LR+++ G ++L + LSK +R KVTG+CGNFN + DD+++ G+D KDI +F SWKTNPTC++ V Y GACA S+ + YA VC+KLY FK CHS + +QC HDVC+CG +R G + CEC AF+SYAR CAL+ L+WR++ LCP KCP M+Y +CGS C TC+ ++ S C EEC+EGC CP G+ DE CV + CKC ++G Y ++R+D+CNNCTCT+GNW CT+ C CP M W +C CQ++C N+++ CPK C+ GC CP+ VLH G+C+ CPC M K+Y+EN + RG C N C+C+ G W C NK C C+ WG+ HYE+FDG+Y++F G+CSYV++ED+CG GTF++ ENI CG+ SCTKS+K Y + V VRG++PI+SRN +P P A Y + T +GI+V+WD M + + + + KVCGLCGNF+ + D+D+RAR+GE+ +D V FADSWKV ++C P P CS +R+ WA+ KC IL+S F CH V V +Y +CV D C C + DDC+C+CTAV QYV RCNE + +KWR ++CP C V+K CG+ CP CIDI K T + CK C+EGCFCPDG ++ C +R+ C CV + K G K+I +C+EC C G+ C + +C T P C +C +CA+RC+G EC G+DE C
Sbjct: 167 NGKCVRGRCVCNLGYKGDKCELPLCKNNCNSVGTCLAPNKCRCPDGYHGDLCQVSALYAS--DSTAFVAVRAVNNTRYRNSAVCSAFGGKHFLTFDGKYFDFPAHGSYVLLQDCVNSEAKYQVYFNGSRNCADA-AQTRCQVSLKIVAGPAKVEIMPGGSV-FVNEKPVTLPTIAGIVSVSNTANHITVRGLDDLAITYDGDRNINVYAPKTMSRTVCGLCGRFDGKAANDFTDLMNKPLTHVVDFAKAWRDESPDDKNVYPSVPADAQHPCARLRSKSTSLLESAQSLCSILRSGSSTFSACHSRVNSSVYESMCMKDMCTCLLNG--NATV--CNEQRCNSATLYSRACTQQGIVINWRQ-SNLCPAPKCSNGKVFSECSHDCNQKCGTNTVDDDCGTHCVDGCFCPSGRFWDGHKCVIRDNCPCNRAGDSRSYPVGSKYSHKCEQCVCDRGGSWKCSPTDECYGYCTVSGDPTYRTFDGRHFSFEGHCEYIMMMPQRS---AAESLEYPVAVYVENKYCDSVTDVLCAKLVTLQMGSGEKQHTIRLESLSRVKVDSAYVSLPVKVH---DITIRLVSTSMLRVETNKGFDLLSD--------LSKAFRNKVTGLCGNFNNDMKDDTISPTGIDVKDIRQFAKSWKTNPTCDAKTRFV-YNGACATSKEFDSYATSVCNKLYTESVFKACHSRVDPKWALEQCKHDVCSCGSRRKGVMSQCECTAFASYARSCALDGKVLNWRSSSLCPVKCP-PTMEYQECGSNCGKTCKRVEASPCIEECVEGCQCPSGQALDEEKGTCVPRNQCKCVYNGVSYTNGQVRKDKCNNCTCTDGNWECTNLTCTDSNTCPAGMVWSECSDCQRSCENINIECPKSSCKSGGCVCPKGQVLHQGKCVDKGNCPCAMAGKVYAENSVVPRGSTRCYNYCMCRKGSWQCTNNK-CPSTCRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMSCTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPCSS--ERKRWAEKKCAILRSSEFTSCHSKVEVNEFYDKCVMDTCAC-KLDDCLCYCTAVSQYVLRCNEMGVKIKWRNPDDCPVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCV--VGGKKIAYGKKIIYKCQECTCTAGSLECKPYYLGDCATFPTTVPPTTTPFCTGPGLIICRGERKYCAKRCDGNRECMYGDDEQCC 1495 HSP 2 Score: 96.2857 bits (238), Expect = 8.566e-16 Identity = 103/467 (22.06%), Postives = 183/467 (39.19%), Query Frame = 2
Query: 200 DCVNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNY-----GAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLG----MELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC 1573
+C CK G C C G ++ GKCV C C N+++ + S + NY G+ NN ST C V H +TFDG + + Y L D G + + C + C+ S+KIT + L + + +N + + G ++ + D +E+ +D + V +K+CGLCG FN DD R + ++ F +SW R+ ++++ + A C +++++ +C S+V+++++ D C + C C +++C +C QY C G+ + WR+ C + CGS C + C + + +C+S C+ GC+CP + + +C +E+C CV + Y G ++ C+ C C G +C
Sbjct: 1014 ECPKSSCKSGGCVCPKGQVLHQ-------------GKCVDKGNCPCAMAGKVYAENSVVPRGS-TRCYNYCMCRKGSWQCTNNKCPST---CRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMS--CTKSVKITYRDVVVHLVRGTDPIISRNLYWPVTLPKAKYEIYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPC--------SSERKRWAEKKCA-ILRSSEFTSCHSKVEVNEFYDKCVMDTCAC--------KLDDCL-CYCTAVSQYVLRCNEMGVKIKWRNPDDC--PVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCVVGGKKIAY--GKKIIYKCQECTC-TAGSLEC 1438 HSP 3 Score: 63.929 bits (154), Expect = 6.156e-6 Identity = 32/76 (42.11%), Postives = 41/76 (53.95%), Query Frame = 2
Query: 191 CSPDCVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGA 415
CSP C NG+C C+CS GW G C+ P+C C+NGG CVAPN C C + G C ++ S V G+
Sbjct: 91 CSPKCDNGMCVAPNSCNCSSGWSGATCNKPVCAKECANGGICVAPNTCRCAAGFDGEQCQSVATTVSSVCVGGCGS 166
BLAST of von Willebrand factor vs. TrEMBL
Match: A0A1I8G0U9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 1087.79 bits (2812), Expect = 0.000e+0 Identity = 558/1363 (40.94%), Postives = 783/1363 (57.45%), Query Frame = 2
Query: 209 NGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRE---REQYPCNALKLKNNDQYQKALTLCQDMVK-NNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVR---GSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLC--PNNFCARRCNGIIECRDGEDEDLC 4267
NG C GRC C+LG++G++C P+C C++ G C+APN+C C D Y G C + +S D + + AV VNNT Y CS G H TFDG F +P G Y L DC+ + + VYFN S +C D Q C +S+KI G + + P V +P + + VS+ + I ++G+DDL I +D DRNI VYAP T + +CGLCG F+G ++D+ D V F +W D P D NVY Q+PC L+ K+ + A +LC + ++ C S V Y MC ++C CL N+T+ C C YS ACT +GI ++WR + +C P+C NG+ FSEC +C + CG ++ +C + C+ GC+CP R+WDG CV ++ CPC R +Y G + CE C+C G W+C +EC G C +SGDP Y TFD RHF F+GHCEY +M + S P+++ ++N C ++ C + + + +G E Q I L S S +KV+ +SLP + + I VST +LR+++ G ++LWNG +RI+I+LSK +R KVTG+CGNFN + DD+++ G+D KDI +F SWKTNPTC++ V Y GACA S+ + YA VC+KLY FK CHS + +QC HDVC+CG +R G + CEC AF+SYAR CAL+ L+WR++ LCP KCP M+Y +CGS C TC+ ++ S C EEC+EGC CP G+ DE CV + CKC ++G Y ++R+D+CNNCTCT+GNW CT+ C CP M W +C CQ++C N+++ CPK C+ GC CP+ VLH G+C+ CPC M K+Y+EN + RG C N C+C+ G W C NK C C+ WG+ HYE+FDG+Y++F G+CSYV++ED+CG GTF++ ENI CG+ SCTKS+K Y + V + +P ++ YP G FY T +GI+V+WD M + + + + KVCGLCGNF+ + D+D+RAR+GE+ +D V FADSWKV ++C P P CS +R+ WA+ KC IL+S F CH V V +Y +CV D C C + DDC+C+CTAV QYV RCNE + +KWR ++CP C V+K CG+ CP CIDI K T + CK C+EGCFCPDG ++ C +R+ C CV + K G K+I +C+EC C G+ C + +C T P C +C +CA+RC+G EC G+DE C
Sbjct: 167 NGKCVRGRCVCNLGYKGDKCELPLCKNNCNSVGTCLAPNKCRCPDGYHGDLCQVSALYAS--DSTAFVAVRAVNNTRYRNSAVCSAFGGKHFLTFDGKYFDFPAHGSYVLLQDCVNSEAKYQVYFNGSRNCADA-AQTRCQVSLKIVAGPAKVEIMPGGSV-FVNEKPVTLPTIAGIVSVSNTANHITVRGLDDLAITYDGDRNINVYAPKTMSRTVCGLCGRFDGKAANDFTDLMNKPLTRVVDFAKAWRDESPDDKNVYPSVPADAQHPCARLRSKSTSLLESAQSLCSILRSGSSTFSACHSRVNSSVYESMCMKDMCTCLLNG--NATV--CNEQRCNSATLYSRACTQQGIVINWRQ-SNLCPAPKCSNGKVFSECSHDCNQKCGTNTVDDDCGTHCVDGCFCPSGRFWDGHKCVIRDNCPCNRAGDSRSYPVGSKYSHKCEQCVCDRGGSWKCSPTDECYGYCTVSGDPTYRTFDGRHFSFEGHCEYIMMMPQRS---AAESLEYPVAVYVENKYCDSVTDVLCAKLVTLQMGSGEKQHTIRLESLSRVKVDSAYVSLPVKVH---DITIRLVSTSMLRVETNKGFDLLWNGRTRIQIRLSKAFRNKVTGLCGNFNNDMKDDTISPTGIDVKDIRQFAKSWKTNPTCDAKTRFV-YNGACATSKEFDSYATSVCNKLYTESVFKACHSRVDPKWALEQCKHDVCSCGSRRKGVMSQCECTAFASYARSCALDGKVLNWRSSSLCPVKCP-PTMEYQECGSNCGKTCKRVEASPCIEECVEGCQCPSGQALDEEKGTCVPRNQCKCVYNGVSYTNGQVRKDKCNNCTCTDGNWECTNLTCTDSNTCPAGMVWSECSDCQRSCENINIECPKSSCKSGGCVCPKGQVLHQGKCVDKGNCPCAMAGKVYAENSVVPRGSTRCYNYCMCRKGSWQCTNNK-CPSTCRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMSCTKSVKITYRDVVVHLPKPLLAGHPAKAKYEIYPAG----FY--------LFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPCSS--ERKRWAEKKCAILRSSEFTSCHSKVEVNEFYDKCVMDTCAC-KLDDCLCYCTAVSQYVLRCNEMGVKIKWRNPDDCPVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCV--VGGKKIAYGKKIIYKCQECTCTAGSLECKPYYLGDCATFPTTVPPTTTPFCTGPGLIICRGERKYCAKRCDGNRECMYGDDEQCC 1494 HSP 2 Score: 93.5893 bits (231), Expect = 6.542e-15 Identity = 105/467 (22.48%), Postives = 185/467 (39.61%), Query Frame = 2
Query: 200 DCVNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNY-----GAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLG----MELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC 1573
+C CK G C C G ++ GKCV C C N+++ + S + NY G+ NN ST C V H +TFDG + + Y L D G + + C + C+ S+KIT ++ PK L+ + K + + G ++ + D +E+ +D + V +K+CGLCG FN DD R + ++ F +SW R+ ++++ + A C +++++ +C S+V+++++ D C + C C +++C +C QY C G+ + WR+ C + CGS C + C + + +C+S C+ GC+CP + + +C +E+C CV + Y G ++ C+ C C G +C
Sbjct: 1022 ECPKSSCKSGGCVCPKGQVLHQ-------------GKCVDKGNCPCAMAGKVYAENSVVPRGS-TRCYNYCMCRKGSWQCTNNKCPST---CRVWGDPHYETFDGRYYDFMGTCSYVLAEDYCGGTAGTFRITGENIKCGAEGMS--CTKSVKITY-RDVVVHLPK---PLLAGHPAKAKYE-----IYPAGFYLFFRTEDGIEVQWDMKMKVSVTLQPNWMEKVCGLCGNFNNKRDDDLRARTGEVVEDKVAFADSWKVRNTCPKPPPTPIAPC--------SSERKRWAEKKCA-ILRSSEFTSCHSKVEVNEFYDKCVMDTCAC--------KLDDCL-CYCTAVSQYVLRCNEMGVKIKWRNPDDC--PVMCPKTMVYKRCGSTCPQRCIDISKGETITSDCKSTCVEGCFCPDGEFLEDGVCKKREQCSCVVGGKKIAY--GKKIIYKCQECTC-TAGSLEC 1437 HSP 3 Score: 51.9878 bits (123), Expect = 8.903e-7 Identity = 85/359 (23.68%), Postives = 154/359 (42.90%), Query Frame = 2
Query: 4322 DQNEIMKSGETKIVDQCIKYICDDGNIVTKTKFCNITCDEHSEILKQHVDSDKCCECVSIRTTTLMVSATTTRLVTCEMYTLTVEKNFNILYKLKSDSIGNSIENVIYDSDKSWTVVSNNAVLIVEFLPKQPXXXXXXXXXXXXXXXXXXXXXDIVEKDDTWKDADSYTLAKSSTKDGNLFSYTFTSATKDGIPGRFVRFSFILSNQITEVIVIRKLMIKICGSQKTTEIQPSSTSGMVSCPPKGTTIRQNECLITMCIDGEWITKMNCPISCLPGEIKVTYDDERCCQCVNANQTTISMPVISSTKLVSTKGPCKPPL-IHKCIDDCEGICHYLQENQCHNLLTSTNRICKDACVCPQ 5395
D++ + S + C Y C G +++ K CN+TC + + + +CCEC + TTT++V+ TTT L C + +T+ + +++++ S N+I+ + K+ AV + +I ++ + A +V ++ D W Y+ ++ L S +F++ T DG+ VR +F + + + L+I C TT + P++T S G +EC+ +C G WI K +C I C V + + CC+C TT + ++ +T C P+ C+ C CHY+ E +C + C+ C CP
Sbjct: 1790 DKDGVKHSEGSTWRSGCAIYTCTKG-VISSRKECNVTCAPGFILRQYPTNPTRCCECERLATTTVIVTPTTTTLTKCTHHEITLASDSGSKFEMRASS--NNIDRL-----KALKGAPLTAVGVQTHRMMSQGFRTIITKLELTATRARSVEVEFTSGSDIW-----YSKRVATPSSSGLVSLSFSNETTDGVSATAVRVTFPRGSGVKVNPSVSGLLISACFEVSTTVVAPTTTPICAS----GDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRK-CHYI-EPECS---SEGKFPCESGCQCPS 2126 HSP 4 Score: 33.4982 bits (75), Expect = 8.903e-7 Identity = 13/37 (35.14%), Postives = 19/37 (51.35%), Query Frame = 3
Query: 5418 CVDTRQCECVDNDGKIRKPNETWTDRCNQYRCVDNQV 5528
CV + C C DGK+R + W C +Y C +N +
Sbjct: 2136 CVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTI 2172 HSP 5 Score: 63.929 bits (154), Expect = 6.062e-6 Identity = 32/76 (42.11%), Postives = 41/76 (53.95%), Query Frame = 2
Query: 191 CSPDCVNGVC-KDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGA 415
CSP C NG+C C+CS GW G C+ P+C C+NGG CVAPN C C + G C ++ S V G+
Sbjct: 91 CSPKCDNGMCVAPNSCNCSSGWSGATCNKPVCAKECANGGICVAPNTCRCAAGFDGEQCQSVATTVSSVCVGGCGS 166
BLAST of von Willebrand factor vs. TrEMBL
Match: A0A0L8GSF5 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22028776mg PE=4 SV=1) HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0 Identity = 368/975 (37.74%), Postives = 543/975 (55.69%), Query Frame = 2
Query: 719 IPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWID-RDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDM-VKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM--CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGE---CIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSG--EIEKDIVNFADSWKVHSSCPVPQKP-PFMCSKRPDREPWAK 3613
+P + +S + ++ +KGIDDL IL+D ++Y +AP K+CGLCG F+G ++++ ++ + F N W+D + + +E +PC L+ K + C + + + C S V D+Y+ MC E+C CL ++++ C C++ QY+ AC+ G + WRS T C C F EC NC ++CG G E ECE C+ GC+C WD + CV + CPC R N + Y G K CE+C C G+W CI +++C G C +SGDPH+ TFD R FFF+G CEY + + S ++ I+N C ++ C + + + G ++ I L S +++VN + I LPY+ + V+I +VS ++RL + G+++LW+G +RI +KLS KY+ KVTGMCGNFN + DD++T G +E ++ KF + WKTN C S + Y GACALS+ Y A +VC+K+++ E FK CH A N KF DQC HDVC CG +R+G CEC AF++YAR C L+ I L+WR + LC C + M Y +CG C TCR +Q C+EECIEGC CP GE +DE CV + +CKC + +Y P RQ+ CN C C +G+W CT+ C + C M W +C C +TC +L++ C C GC CPE V N + C++P ECPC Y D I + +C N C CK G W C + + C+ C+ +G+ HY +FDGK + FQG+C+Y++++DFC GTF++ +EN+ CG+ SCTKS+KF+ + + RG+N + N P G P A + +V T +I+ GI + WDK+M L I + KY +VCGLCGN+D +ND R +S E + + FA+SW+V SC + K P C R+ WA+
Sbjct: 19 LPHQVGDLELSEIANYTVVKGIDDLIILYDGLSSVYAFAPPQMLGKLCGLCGQFDGNKTNEFVNYVRKPVATKADFSNVWLDPMEQRSIPSVKEDSVHPCVRLRNKFSKAADNVEAFCSVFSLDYVVFQKCHSTVPPDEYIAMCSREMCTCLL----GNSLQECSHKRCEVATQYARACSHHGKIMKWRS-NTFCPPHTCPKNMVFKECSKNCRQSCGSEGFEAECEEFCVDGCFCKEGTLWDTSTSSCVHRGDCPCYRGNDQRAYPIGTLEKSGCESCTCAEGGRWNCIMSKDCPGICTLSGDPHFQTFDGRQFFFEGECEYVLSQYQAKDGGES------FAVWIENKYCNKITDAVCTKVVTIKFG-SKNNTQIIRLRSFKVEVNSMSIGLPYK---DDDVVIRKVSNILIRLTASIGLDVLWDGRTRIYLKLSNKYQGKVTGMCGNFNNDVNDDNLTPNGENEFNVTKFAMEWKTNTACNSRPSTI-YLGACALSKQYEKLATEVCNKMWKEESFKSCHFAVNPSKFVDQCKHDVCMCG-QRHGDPRDCECAAFATYARACVLKGITLNWRKSNLCFINC-TKGMIYQECGYNCDQTCRGIQPQNCEEECIEGCNCPPGEKYDEEDNVCVPVEECKCYYRNEYYEPKSSRQELCNECICIDGHWQCTTESCNVHDLCSGDMVWSNCSDCIRTCDSLNVDC-VATCSQQGCICPEGKVQLNKDSQNCVEPSECPCNHLGYQYKNGDKIKK---DC-NQCYCKHGTWKCTE-RHCAATCEAFGDPHYVTFDGKPYNFQGACAYIVAQDFCNETGGTFQVTTENVPCGTLGYSCTKSVKFLIYDTIINLARGTNITYTINNKAPEGTPMANFTIKEVGT-YIMIEAHGITLVWDKSMRLYITLENKYMGRVCGLCGNYDQIANNDFRDKSSFPEHTQSPLAFANSWRVRESCAIVDKELPNPCVLNSHRQSWAE 968 HSP 2 Score: 165.622 bits (418), Expect = 2.210e-37 Identity = 142/472 (30.08%), Postives = 219/472 (46.40%), Query Frame = 2
Query: 2747 CPKSMAWFDCGI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVL--HNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXX-XXCTK--SIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI------LFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFM--CSKRPDREPWAKAKC-EILKSDSFKKCHDVVNVEHYYKRCVADACECDR--GDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW--QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
CPK+M + +C C+++C + + + GCFC E T+ C+ +CPCY N + + + C++ + G+W C +K C GIC G+ H+++FDG+ F F+G C YV+S+ + +F + EN C + + CTK +IKF N Q +R + V N+ G P Y DV + L G+ V WD + + + KYQ KV G+CGNF+ +++D +GE E ++ FA WK +++C ++ C+ E A C ++ K +SFK CH VN + +C D C C + GD C C A Y C I L WR + C CT +++ CG C +C I C++ CIEGC CP G + ++N CV + C+C N Y C EC C +G ++CT
Sbjct: 213 CPKNMVFKECSKNCRQSCGSEGFEAECEEFCVDGCFCKEGTLWDTSTSSCVHRGDCPCYRGNDQRAYPIGTLE-KSGCESCTCAEGGRWNCIMSKDCPGICTLSGDPHFQTFDGRQFFFEGECEYVLSQYQAKDGGESFAVWIENKYCNKITDAVCTKVVTIKFGSKNNT-QIIRLRSFKVEVNS-MSIGLP---YKDDDVVIRKVSNILIRLTASIGLDVLWDGRTRIYLKLSNKYQGKVTGMCGNFNNDVNDDNLTPNGENEFNVTKFAMEWKTNTACNSRPSTIYLGACALSKQYEKLATEVCNKMWKEESFKSCHFAVNPSKFVDQCKHDVCMCGQRHGDPRDCECAAFATYARACVLKGITLNWRKSNLCFINCTKGMIYQECGYNCDQTCRGIQPQ-----NCEEECIEGCNCPPGEKYDEEDNVCVPVEECKCY--YRNEYYEPKSSRQELCNECICIDGHWQCT 671 HSP 3 Score: 107.071 bits (266), Expect = 2.629e-19 Identity = 110/399 (27.57%), Postives = 175/399 (43.86%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFM-DCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQ--TIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGS-FIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW-----IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALT-LCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWD--GTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGD 1618
C++ H +TFDG F + Y L F V+ Y ++ T C+ + I G++ T I+ ++ V + +P+ + +++ V + I++ L++L+D IY+ + K+ G+CG FN ++DD N + NV F W + PS + + A L + QY+K T +C M K ++C V K++D C+ ++C C Q + E C Y+ AC LKGI ++WR + +C C G + ECG NC + C + + CE +C+ GC CP +D +CVP E+C C N E + R +L C CIC + G WQC E C SGD
Sbjct: 312 CTLSGDPHFQTFDGRQFFFEGECEYVLSQYQAKDGGESFAVWIENKY--CNKITDAVCTKVVTIKFGSKNNTQIIRLRSFKVEVNSMSIGLPYKDDDVVIRKVSNILIRLTASIGLDVLWDGRTRIYLKLSNKYQGKVTGMCGNFNNDVNDDNLTPNGENEFNVTKFAMEWKTNTACNSRPSTIYL---------GACAL--SKQYEKLATEVCNKMWKEESFKSCHFAVNPSKFVDQCKHDVCMCGQRHGDPRDCE------CAAFATYARACVLKGITLNWRK-SNLCF-INCTKGMIYQECGYNCDQTCRGIQPQ-NCEEECIEGCNCPPGEKYDEEDNVCVPVEECKCYYRN-EYYEPKSSRQEL-CNECIC-IDGHWQCT-TESCNVHDLCSGD 684 HSP 4 Score: 95.5153 bits (236), Expect = 9.981e-16 Identity = 77/285 (27.02%), Postives = 119/285 (41.75%), Query Frame = 2
Query: 3323 SDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSW----KVHSSCPVPQKPPFMCSKRPDREPWA----KAKCEILKSD--SFKKCHDVVNVEHYYKRCVADACECDRGDDCV----CFCTAVEQYVARCNEYEIGLKWRTNENC-PHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW--QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKEC 4126
S++ + ++ ++ + + +D S+ P + K+CGLCG FD N+ + +F++ W + S V + C + ++ A +A C + D F+KCH V + Y C + C C G+ C QY C+ + +KWR+N C PHTC V+K C C SC G C++ C++GCFC +G W + CV R C C Y G CE C C EG R + SK+C
Sbjct: 29 SEIANYTVVKGIDDLIILYDGLSSVYAFAPPQMLGKLCGLCGQFDGNKTNEFVNYVRKPVATKADFSNVWLDPMEQRSIPSVKEDSVHPCVRLRNKFSKAADNVEAFCSVFSLDYVVFQKCHSTVPPDEYIAMCSREMCTCLLGNSLQECSHKRCEVATQYARACSHHGKIMKWRSNTFCPPHTCPKNMVFKECSKNCRQSCGSEGFEAE----CEEFCVDGCFCKEGTLWDTSTSSCVHRGDCPCYRGNDQRAYPIGTLEKSGCESCTCAEGG-RWNCIMSKDC 308
BLAST of von Willebrand factor vs. Ensembl Cavefish
Match: vwf (von Willebrand factor [Source:NCBI gene;Acc:103041546]) HSP 1 Score: 539.265 bits (1388), Expect = 1.842e-157 Identity = 362/1275 (28.39%), Postives = 564/1275 (44.24%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVS-SVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRL--LQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEF------------------CPKSMAWFDC------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQF----VRGSNPIVSRNTNYPPGGPTAFYWTSDVDT-----HFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKR-------VWK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDG--LHWQNNECVERKAC-------ECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
CS+ HH+ TFDG ++ +P Y L DC + F+ D + + + + +G + + +P+ S + +GS I D NI + CGLCG +N + +D+Y + ++ F NSW S+ CN D ++ C+ + + + C V D + +C+ ++C C+ Q C+ +Y+ C G+ + +W + + P+C G ++SEC C +C L + C +C+ GC C + DG CV +C C + Y G + +C TC+C HG W+C NEEC G+C +SG HY TFD + F F G C+Y N+ S I C D+ + C R++ + E +Q + L + V+ + + +P N + + +RL +++ W+G R+ ++LS Y K+ G+CGN+NGN+ DD +T G+ E ++ F +WK N C++ + + C+L+ +A + C L S +F+ CH N + C +DVC+C + C C A S+YA C+ + ++WR C CP N Y CGS C TCR L +S C+ C EGCFCP G + ECV C C + G Y P ++ D + C C NG+ C+S++ CP + C GI C +TC NLDL C C + GC CP TV H EC+ P +CPC+ NN YS +I +C N C+C +W C Q + C G+C T G++HY SFDG F F G C Y++++D+C GTF+++ EN ACG+ C KSI Y+ + V+ P++ +DV+ ++I+ + I + WD + + I +Y+ KVCGLCGNFD +ND+ + + +IE D ++F +SWKV SC + P CS ++ + C +L SD F++C+ VV+ E Y++ C D C C DC CFC A+ Y C + + + WR+N+ CP +C +W+ C CP +C + C C+EGC CP G L CVE C E + + N GD + C+ C+C E C
Sbjct: 27 RCSLFGRHHVHTFDGVIYEFPGDCSYKLAGDC------HHRTFSILGDLSHGRRKG-----VTVFLGETFELHLSVGGVLSQGAERLPLPYASSSVFAGFELGSVRLWSEEFGFSIRIDPAHNIQLTLSKHHANHTCGLCGNYNSVPTDEYTAQEGFLTEDPYDFANSWAMNGASEACKRVTPPAQTCNNTGASARD----LMSRCETLRTSPVFLHCAHLVDTDAFAAVCESDVCHCVDGQ----------ECVCQALLEYARTCASHGLVLPNWHTQSQC--TPKCPIGMQYSECSQACSTSCQSLNIHEVCREECVDGCSCAVGKVLDGDRCVEVSQCSC--QHTGKRYPPGSTISQDCNTCVC-RHGSWECT-NEECPGQCVVSGQSHYKTFDDKFFTFSGFCQYLLAKDCQTNLF---------SAIIDTVQCADDQDAVCTRSVTLKFHELANQT-VRLKHGGVVSVDGLDVQMPLI---NGLLRVQSTVLSSVRLNYGDDLQLDWDGRGRVVLRLSPVYAGKMCGLCGNYNGNQGDDFLTAGGLVETRVEGFGNAWKMNAECDNIQK--QPSDPCSLNPKRVRFAEEAC-ALLLSPKFEPCHHEVNPYPYLKNCRYDVCSCADGQE-----CLCAAVSNYAAACSARGVLINWRGQGYCELTCPG-NQVYEVCGSVCNQTCRSRSLPDSACRSMCEEGCFCPRGLYLTDTG-ECVPPDQCSCHYDGEIYQPNDMFADHHSICYCENGSMRCSSSELPASVLSDLFFDEPPLSRERRSVSCPAPLQQLRCESVRDVGIECARTCQNLDLECVSQGC-ISGCVCPPGTVRHRRECVPPDQCPCFHNNHAYSPGQSI---KIDC-NTCVCGGRQWKCTQ-RVCDGVCHTVGDSHYISFDGLKFTFPGPCQYILAQDYCNGLDGTFRILVENSACGTIGHRCRKSISVFYMGGLIVMEQEEVKMKKPVMKE---------------TDVEIVRSGFYYIVLLGKNISITWDTGTRIILQISGQYRGKVCGLCGNFDGSQNNDLLSSNNQIEVDPLDFGNSWKVQPSCANAVQVPSQCSTDMSKQVTVEQSCRVLNSDLFRECNSVVDPEPYWEICTHDTCSCASVGDCACFCDAIAAYAHECAQKGVVVDWRSNDLCPLSCEELNKDAGVECMWRFNACDTACPKTC-----QHPEQLSCPIACLEGCHASCPQGQILDEVLRRCVEPSECQVCMHNGERISHGKQIILNHGDPHL--CQICHCEENTLTCA 1219 HSP 2 Score: 144.821 bits (364), Expect = 5.904e-34 Identity = 106/384 (27.60%), Postives = 162/384 (42.19%), Query Frame = 2
Query: 1595 GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK 2740
GRC + G H TFD + F G C Y D + S + LS G + + V +GE + ++L + ++ LPY ++ V + E G I + I++ LSK + G+CGN+N D+ +G +D F SW N E+ + + C + + + C L S F C + D F C DVC C + C C+A YAR CA + L +W T C KCP M+Y++C C+++C+ L C+EEC++GC C G+ D CV++S C C+ G+ Y P CN C C +G+W CT+ +C G+
Sbjct: 26 GRCSLFGRHHVHTFDGVIYEFPGDCSYK---LAGDCHHRTFSILGDLS-------------HGRRKGVTVFLGETFE---LHLSVGGVLSQGAERLPLPY---ASSSVFAGFELGSVRLWSEEFGFSIRIDPAHNIQLTLSKHHANHTCGLCGNYNSVPTDEYTAQEGFLTEDPYDFANSWAMNGASEACKRVTPPAQTCNNTGASARDLMSRCETLRTSPVFLHCAHLVDTDAFAAVCESDVCHCVDGQE-----CVCQALLEYARTCASHGLVLPNWHTQSQCTPKCPI-GMQYSECSQACSTSCQSLNIHEVCREECVDGCSCAVGKVLD--GDRCVEVSQCSCQHTGKRYPPGSTISQDCNTCVCRHGSWECTNEECPGQ 379 HSP 3 Score: 132.109 bits (331), Expect = 4.216e-30 Identity = 96/369 (26.02%), Postives = 166/369 (44.99%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME------GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS---SCPVPQKPPFMCSKRPDREPWAKAKCEILK-SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
G C +G H +FDG +EF G CSY ++ D C ++ TF ++ G S K + ++L F+ ++S+ P Y +S V F L ++ G ++ D A ++++ + + + N CGLCGN++ ++ A+ G + +D +FA+SW ++ +C P C+ ++CE L+ S F C +V+ + + C +D C C G +CV C A+ +Y C + + L W T C C + C C SC +++ + C++ C++GC C G + CVE C C T +Y G + ++C C C G++ CT
Sbjct: 26 GRCSLFGRHHVHTFDGVIYEFPGDCSYKLAGD-CHHR--TFSIL------GDLSHGRRKGVT-VFLGETFELHLSVGGVLSQGAERLP----LPYASSSVFAGFELGSVRLWSEEFGFSIRIDPAHNIQLTLSKHHANHTCGLCGNYNSVPTDEYTAQEGFLTEDPYDFANSWAMNGASEACKRVTPPAQTCNNTGASARDLMSRCETLRTSPVFLHCAHLVDTDAFAAVCESDVCHCVDGQECV--CQALLEYARTCASHGLVLPNWHTQSQCTPKCPIGMQYSECSQACSTSC----QSLNIHEVCREECVDGCSCAVGKVLDGDRCVEVSQCSCQH--TGKRYPPGSTISQDCNTCVCRHGSWECT 372 HSP 4 Score: 112.079 bits (279), Expect = 5.259e-24 Identity = 124/479 (25.89%), Postives = 192/479 (40.08%), Query Frame = 2
Query: 191 CSPDCVNGVCKDGRCSCSLG--WQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPF-NSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW--------IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGM-ELECESQCLPGCYCPRNRYW-DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENE 1585
C +CV+G CSC++G G+RC + + C + GK P + D CNT + + + +N +C V H KTFD F++ Y L DC LF + DQ S+++K + V ++P N + S+V S +++ DDL++ +D R R + +PV A K+CGLCG +NG DD+ +++ V+ F N+W I + PSD PC+ N + + A C ++ C EV YL C+ ++C C Q C Y+ AC+ +G+ ++WR + C Q + CGS C + C + + C S C GC+CPR Y D CVP ++C C D GE YQ + C C +G +C +E
Sbjct: 313 CREECVDG------CSCAVGKVLDGDRCVE-VSQCSCQHTGKRYPPGSTISQD------CNTCVCRHGSWECTNE-----------ECPGQCVVSGQSHYKTFDDKFFTFSGFCQYLLAKDC--QTNLFSAIIDTVQCADDQDAVCTRSVTLKFHELANQTVRLKHGGVVSVDGLDVQMPLINGLLRVQSTVLSSVRLNYGDDLQLDWDGRGRVVLRLSPVYA-GKMCGLCGNYNGNQGDDFLTAGGLVETRVEGFGNAWKMNAECDNIQKQPSD----------PCSL----NPKRVRFAEEACALLLSPKF-EPCHHEVNPYPYLKNCRYDVCSCADGQ----------ECLCAAVSNYAAACSARGVLINWRGQGYC--ELTCPGNQVYEVCGSVCNQTCRSRSLPDSACRSMCEEGCFCPRGLYLTDTGECVPPDQCSCHYD-GE-IYQPNDMFADHHSICYC-ENGSMRCSSSE 734 HSP 5 Score: 92.4337 bits (228), Expect = 4.350e-18 Identity = 117/441 (26.53%), Postives = 184/441 (41.72%), Query Frame = 2
Query: 308 KCVAPNQCLCLDN---YSGP-----NCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMD-CLGAAPLFYVYF-NASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRS---ATTICKQPQCFNGQE----FSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYWDGTM--CVPQEKCPCVRDNGESTYQQGYRLKLN------CETCIC 1549
+CV P+QC C N YS +CNT + Q + RV + + C + H +FDG F++P Y L D C G F + N++ + + SIS+ G +I+ + E + K + ++ IV S +I + G ++ I +D I + K+CGLCG F+G ++D N I+ + F NSW PS N + Q + K +Q + L ++L R C S V + Y ++C + C C +++ +C + FC Y++ C KG+ VDWRS C++ G E F+ C + C K C Q +L C CL GC+ CP+ + D + CV +C NGE G ++ LN C+ C C
Sbjct: 810 ECVPPDQCPCFHNNHAYSPGQSIKIDCNTCVCGGRQ-----WKCTQRVCDGV------CHTVGDSHYISFDGLKFTFPGPCQYILAQDYCNGLDGTFRILVENSACGTIGHRCRK--SISVFYMGG---LIVMEQEEVKM----KKPVMKETDVEIVRSGFYYIVLLG-KNISITWDTGTRIILQISGQYRGKVCGLCGNFDGSQNNDLLSSNNQIEVDPLDFGNSW-KVQPSCANAVQVPSQCSTDMSKQVTVEQSCRVL-------NSDLFRECNSVVDPEPYWEICTHDTCSC-------ASVGDC-ACFCDAIAAYAHECAQKGVVVDWRSNDLCPLSCEELNKDAGVECMWRFNACDTACPKTC-QHPEQLSCPIACLEGCHASCPQGQILDEVLRRCVEPSECQVCMHNGER-ISHGKQIILNHGDPHLCQICHC 1211 HSP 6 Score: 72.4034 bits (176), Expect = 6.126e-12 Identity = 105/481 (21.83%), Postives = 183/481 (38.05%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSET-GVEILWNGGSR---IEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCH--KLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTC---------------RLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCK--CEFHGRFYLPYE---IRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQ--------KTCTNLDLVCPKGQCQL---PGCFCPENTVLHN-GECIKPFECPC 2911
+C C S H ++F G C Y + + ++L +T C + C +++ + E ++ L ++ V+ ++P++ G V + Q+ + +L+SET G + + S I + L+ TG+CG+ + ++ + G D D ++ +W G C +++ S V +L S F +CH+ + + C C A+ C+ ++Y+ C + + ++WR +LC +K +M+Y+ C + C C L S+C EGCFCP G E ECV C + HG+ + E + C C C + I CT+ C K+ A CG C+ K C + VC C++ P C + VL N GEC ++C C
Sbjct: 1954 KCPCMCMGSSTSHVVSFGGTALKLKGACSYLLLHSGGTELVLHTAT------------CDSSPNQICMKSLEL----KEGGTSVMLKDDMKVTVDGAVTAVPFRRGG---VSVTQIGALLHQLRSETQGFTLTFTPHSNEFTISLDLTMITNTSTTGICGSCSDDQAH-LLLPDGSPSTDPDAYLQAWAV--------------GECVWNKNVSVCEAGVAQGCELLASSVFGQCHAVLPPQDYVEMCKQLAC-----HPTAM----CDLITAYSSVCRQQGVCVNWRNADLC-RKSLLFSMEYDACRTGCVEQCGHGQAALGDMDVRGDGLGGSSQCWSAPTEGCFCPHGTVLLEG--ECVGREACSQCVDQHGQAHQLMESWTPNDNPCILCVCLDKQRINCTALPCT------KAKAPV-CGKCEILLEKTDSKCCPEYECVCDTANCKVSDPPQCENGLSPVLTNPGECAPIYQCVC 2381 HSP 7 Score: 65.0846 bits (157), Expect = 8.508e-10 Identity = 86/388 (22.16%), Postives = 143/388 (36.86%), Query Frame = 2
Query: 2945 TIVRGPANCQN--PCICKDGKWVCEQNK------QCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIAC-GXXXXXCTKSIKF-------IYLNRVFQFVRGSN---PIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEI-MIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSC---------IDI-GKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVD 4015
T++ NC+ P +CK+ N+ +C +C +H SF G + +G+CSY++ G +L+ C S + C KS++ + + + V G+ P + G S+ + FT + +SL++ MI +CG C + + G D + +W V V K +C E CE+L S F +CH V+ + Y + C AC C + Y + C + + + WR + C + + C C + C +D+ G + ++ C EGCFCP G ECV R+AC +CVD
Sbjct: 1920 TLLSHRVNCERMEPPVCKNNLRAVRVNQTCGCSWKCPCMCMGSSTSHVVSFGGTALKLKGACSYLLLHS------GGTELVLHTATCDSSPNQICMKSLELKEGGTSVMLKDDMKVTVDGAVTAVPFRRGGVSVTQIGALLHQLRSETQGFTLTFTPHSNEF----TISLDLTMITNTSTTGICGSCSDDQAH----LLLPDGSPSTDPDAYLQAWAVGEC--VWNKNVSVC------EAGVAQGCELLASSVFGQCHAVLPPQDYVEMCKQLACHP------TAMCDLITAYSSVCRQQGVCVNWRNADLCRKSLLFSMEYDACRTGCVEQCGHGQAALGDMDVRGDGLGGSSQCWSAPTEGCFCPHGTVLLEGECVGREACSQCVD 2279
BLAST of von Willebrand factor vs. Ensembl Cavefish
Match: scospondin (subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]) HSP 1 Score: 493.812 bits (1270), Expect = 2.548e-142 Identity = 359/1267 (28.33%), Postives = 546/1267 (43.09%), Query Frame = 2
Query: 359 NCNTLLIKSSQSD--VSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSEC--GSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFD-GHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWN-GGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEE------CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLP--GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIV-SRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKV-CIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
+C+ L+ Q+ + G + ++N S C G H ++FD F + Y L G + VY + D CS ++++ +G + + N P G+ + +G F+ ++ + I FD +Y+ GLCG +N DD+ + I + +F NSW D E ++ + + ++ +K + C +V Y+D C C + + C Y+ C + I + WR +C++ C GQ FS+C + +C +C+ GC CP Y C+ ++ CPC TYQ+G +K C TC+C G+WQC +E C +C I G H TFD++ + G C++ V D L ++TI+ C TGCP+ + V + + S + +N +LP T L + +T L G +LW+ GS + I L + KV G+CG N+ DD T +G E + F + +C + C+ YA VC L+ S F+ CH + + C +VC+C R C C ++YA+ CA E + +SWR + CP +C + Y +CG C +C LQ+S EE C+ GC CPEG D+ +CV + C C G + P Q CN C C G W C+ + C CP S+ F G C +TC++LD P+ C P GC CPE TVL +C+ P ECPC+ N +++ NDTI + +C N C+CK +W C Q + C+G C G+ HY +FDG+ + F G C Y+++++ G F + +EN+ CGS+ +CTKS+ N +RG + V P + V L + G+ + WD M + + + Q +V GLCGNFD +ND R G +E F +SWKV SCP Q C P R WAK KC ++ + F CH V + YY CV DAC CD G DC C CTA+ Y CN + + WR+ E CP C V++ CG+ C C +V ++ C + C+EGCFCP G + C+ C C + G + + C+ C C G + CT
Sbjct: 165 SCSYTLLPDPQTSPLLLRKGHLGGLHNPKASA--TCMSWGGVHYRSFDRKHFHFQGGCTYVLASSTDGT---WAVYISTVCD-----GGGHCSKALQMMLGRDLVSVNKSNMSLNGLIIAQGEPVFQNGVSIHWLGDFVFVESGLGVRIKFDLSNTVYLTVNTEHFAATRGLCGVYNNNAEDDFTGRSGSISQYAASFGNSWRVPDQQTEGCSDAAELG--HSCDVTGDVSLRRGAESACHQLKESPFSHCHHQVDPGPYVDTCLYVYCSLGEKER--------VGAVCDTLASYARECAQQHIILSWRRPD-LCER-VCPGGQLFSDCVSSCPPSCSSALPPASGQCREECVGGCECPPGLYLHRGQCLRKDDCPCFHR--RHTYQEGDTIKQKCNTCVC-QGGQWQC-SSERCAAQCSIIGAMHISTFDKKSYNLQAGGCQFIVVEDFVDKKL---------AVTIRAGECVTGGRTGCPQEMSVTA----LHTTVTVNSKGSVILNGQPEALPVL----TADLAVRRATSSFLLVQVFGARLLWHTDGSLVVITLEPGFAHKVRGLCGTLTWNQHDDFTTPEGDVENSVPSFAAKFNIG-SCPLSSAVT--SDPCSTYTQRRQYAESVCAVLH-SAVFQACHDVVEREPYVRMCQTEVCSCATHR-----LCHCTVVTAYAQHCAQEGVLVSWRNHTFCPVQCSGGQV-YQECGRACGGSCADLQQSWVCEENAGSRTCVPGCQCPEGLMQDDQG-QCVPVDMCPCVHGGAVHQPGSSVQINCNTCVCGKGVWNCSDDPCPEVRECPGSLI-FSPGSCLRTCSSLDS--PQALCTEPIYGCVCPEGTVLLGDQCVTPDECPCHHNGRLFFTNDTISK---DC-NTCVCKQRRWHCTQ-QLCAGTCVATGDPHYVTFDGRCYSFLGDCEYMLAQEM----SGLFSVSAENVPCGSTGVTCTKSVTLTVGNTAIHLLRGKSVTVNGMPVTLPKSYSGSGLVLEKVGMFVSLSSRLGVTLLWDGGMRVYVRLEPHLQGRVGGLCGNFDGDTENDFTTRQGIVESTPELFGNSWKVSPSCPDVAEQDLRDPCVINPHRVTWAKKKCAVITQELFSLCHAEVPYQQYYDWCVFDACGCDSGGDCECLCTAIAAYAEECNRRGVYIHWRSQELCPLQCEDGLVYEPCGSTCSAVCPSSTPSV--HSHCSPLSCVEGCFCPHGTVRHGDGCITPSECPCE--WEGSLFPAGAVLTQHCQNCTCSNGTWDCT 1361 HSP 2 Score: 56.9954 bits (136), Expect = 2.840e-7 Identity = 36/92 (39.13%), Postives = 44/92 (47.83%), Query Frame = 2
Query: 3836 GKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITN--LKYNTGDKVIRECEECNCYEGAFRCT 4105
G+ NC N CP SC D+ ++V G C GC CPDG Q ECV AC C P N L+ + G V +C C C G+ CT
Sbjct: 4500 GRVYEANCANQCPRSCADLWEHVQCLQG---TCHPGCRCPDGWLLQEGECVRVTACRCGLPTENGTLELSPGQNVTVDCNTCVCINGSLVCT 4588 HSP 3 Score: 55.8398 bits (133), Expect = 6.200e-7 Identity = 58/211 (27.49%), Postives = 84/211 (39.81%), Query Frame = 2
Query: 2441 LCP-KKCPNENMKYNQ-CGSRCTSTCR-LLQESKCKE-ECIEGCFCPEGETFDEAAKECVKISDCKCEF---HGRFYL-PYEIRQDRCNNCTCTNGNWICTSNDCEG-------------------------------------------KEFCPKSMAWFDC-GICQKTCTNL--DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPC 2911
LCP ++C + Y C ++C +C L + +C + C GC CP+G E ECV+++ C+C +G L P + CN C C NG+ +CT+ C + CP + C G C +C +L + C G C PGC CP TV+ NG C+ ECPC
Sbjct: 4490 LCPGERCEDRGRVYEANCANQCPRSCADLWEHVQCLQGTCHPGCRCPDGWLLQEG--ECVRVTACRCGLPTENGTLELSPGQNVTVDCNTCVCINGSLVCTNQPCPTYGSWGSWSECSVSCGTGQRIRRRSCTHKPGGPPCSETEQNQTCTQPCPAGQVFSACAGSCPFSCQDLWPESECVPGACS-PGCACPPATVMFNGTCVPHSECPC 4697
BLAST of von Willebrand factor vs. Ensembl Cavefish
Match: ENSAMXT00000041370.1 (pep primary_assembly:Astyanax_mexicanus-2.0:5:1809499:1822580:1 gene:ENSAMXG00000037407.1 transcript:ENSAMXT00000041370.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 454.521 bits (1168), Expect = 5.292e-131 Identity = 357/1234 (28.93%), Postives = 542/1234 (43.92%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDF-NQVIQKNVKTFVNSWIDRDPSDL-NVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWD---GTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGV--EILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYE-SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPK---KCPNENMKYNQCGSRCTSTCRLLQES--KCKEE--CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK-EFCPKSMAWFDCGI---------CQKTCTNLDLVCPKGQCQ-----LPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP---VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC-----TGKRVW--KNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACEC 4009
CS +H KT+DG+VF P + L C G+ Y FN ++ +N KIT+ ++ K P G+ + ++ S+I +K L +++ D + + + CGLCG FNG+ + Y +F Q + + N W P++ ER C N KA + C D+V ++ + +C +++C+C NS+ + S C +YS C G +WR+ C +C + + ECGS C C C C+ GC+CP + +D T C+P C C+ +G Y G +C C C G+W C++ ++C G C + G H ITFD + + F G C Y VL T +I C C + + + + N+ S E+ +N + + + + I + S+ + +++ G+ EI ++ I K++ ++ G+CGNFN + DD T G+ E F +WKT +C + ++ C+LS YA C L + S F CHS N + C +D C C + C C A SSY CA + I + WR + C CPN M Y+ + C +CR L ++ C + ++GC C EG +E +ECV S C C P E CTC G C + GK + C M + +C C K+C LD+ C + + GC CP V NG CI CPC N Y +T+ +C N C CKD +W C N QCS C +G+ HY +FD K + F G+C Y +++DFC N GTF++I+ENI CG++ ++C+K+IK + GS +V R+ G Y + + ++ T G+ + WD+ S+ I + + +VCGLCGNFD +ND RS E+ + ++F +SWK +CP V Q+P C+ P RE WA+ +C I+ SD F CH V+ +Y CV DAC CD G DC CFCTAV Y CN + + WRT + CP C G+ W K CG C +C +N + +EGC+ CP GL + + + CV + C C
Sbjct: 29 CSTWGNYHFKTYDGDVFQLPSTCNHILTSQCRGS----YEAFNIQ---LRRQVENGEPTISKITMKLDGTVV------------DVKAPHLGSGVFIETISSYIMVKATVGLTAMWNEDDALMIELNAKFINQTCGLCGDFNGVPT--YNEFIKDGAQLSATDYGNLWKMDGPTETCEEQPERVNENCGDENFCQNLFLSKAFSSCTDLVS------------VEPFTKVCIEDLCQC------NSSAGS--SCLCDTLAEYSRQCVHAGGQPANWRTEH-FCPM-KCPESKVYMECGSPCMDTCSNPERGQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCI--HGGKAYASGEGYSSSCRECTCS-GGQWNCVD-KQCPGTCSVEGGAHIITFDGKPYTFHGDCSY---------VLSKECTGTDFTILGDLVKCGITDSETCLKAVTLALSN-----GTNVSLSCELNINLLFKKIFRLTQTESDISIFKPSSFYIVIQTTIGLRLEIQLTPIMQVYITADPKFQGQMCGLCGNFNNIQADDFTTMGGLTEGTAVDFANTWKTRASCLDVQRT--FENPCSLSTENEKYAQYWCSLLSDASGVFSSCHSEINPATY-KNCIYDTCNCEKTED-----CMCAALSSYVHACAAKGIYIKGWR-DVACKSYTLSCPN-TMVYSYNMTSCGRSCRSLSQTDFSCSSQFVAVDGCGCAEGTYLNEK-EECVDPSGCPCYDSNTVIQPGETVSREGTTCTCKQGQLNCIGS---GKVQECTSPMTYLNCSAAVPGSTGSECAKSCKTLDMACVSYHIKYTRGCVSGCVCPSGLVSDDNGGCITEDLCPCVHNGASYKPGETVKE---DC-NTCTCKDRRWQCTTN-QCSATCSIYGDGHYITFDDKRYVFNGNCEYTLAQDFCSSSNTNGTFRVITENIPCGTTGTTCSKAIKLFIGSNELVLSDGSYQVVQRD-----AGQEIPYQIRTMGNYLVIETNNGVVLIWDRKTSMYIKLTPDFNGRVCGLCGNFDGNANNDFTTRSQELVVNALDFGNSWKFSPTCPEAMVVQEP---CAHNPYREAWAQRQCSIINSDVFSACHSKVDPASFYSACVRDACACDTGGDCECFCTAVAAYAEACNAVGVCVAWRTPKVCPLFCDYYNPPGECEWHYKPCGAPCMKTC----RNPAGTCSLQLPAMEGCYPECPAGLPFFDEDTMKCVALEECGC 1170 HSP 2 Score: 169.859 bits (429), Expect = 1.036e-41 Identity = 141/488 (28.89%), Postives = 222/488 (45.49%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTV---LHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXX-XXXCTKSIKF-----------------IYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGI--QVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKP-PFMCSKRPDREPWAKAKCEILKSDS--FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRT------NENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNT-GCKKVCIEGCFCPDGLHW-QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC 4102
CP+S + +CG C TC+N + G+ + GCFCP +TV + N CI C C K Y+ + G ++ C C G+W C +KQC G C G H +FDGK + F G CSYV+S++ G F ++ + + CG + S +C K++ + ++F+ + + I P++FY ++ T G+ +++ M + I K+Q ++CGLCGNF+ +D G E V+FA++WK +SC Q+ CS + E +A+ C +L S F CH +N YK C+ D C C++ +DC+C A+ YV C I +K WR +CP+T +CG SC + + TD + + V ++GC C +G + + ECV+ C C D +N G+ V RE C C +G C
Sbjct: 273 CPESKVYMECGSPCMDTCSNPERGQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCIHGGKAYASGE----GYSSSCRECTCSGGQWNC-VDKQCPGTCSVEGGAHIITFDGKPYTFHGDCSYVLSKECTGTD---FTILGDLVKCGITDSETCLKAVTLALSNGTNVSLSCELNINLLFKKIFRLTQTESDISIFK-------PSSFY--------IVIQTTIGLRLEIQLTPIMQVYITADPKFQGQMCGLCGNFNNIQADDFTTMGGLTEGTAVDFANTWKTRASCLDVQRTFENPCSLSTENEKYAQYWCSLLSDASGVFSSCHSEIN-PATYKNCIYDTCNCEKTEDCMC--AALSSYVHACAAKGIYIKGWRDVACKSYTLSCPNTMVYSYNMTSCGR----SCRSLSQ--TDFSCSSQFVAVDGCGCAEGTYLNEKEECVDPSGCPCYD--SNTVIQPGETVSREGTTCTCKQGQLNC 726 HSP 3 Score: 119.783 bits (299), Expect = 1.926e-26 Identity = 92/375 (24.53%), Postives = 156/375 (41.60%), Query Frame = 2
Query: 3011 EQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKC-------EILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARC-NEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQ---NNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC 4102
E N Q +C TWG H++++DG F+ +C+++++ C F NI + +I I + V + P G F T + ++ ++ G+ W++ +L I + K+ N+ CGLCG+F+ + + G + ++ + WK+ P C ++P+R C + S +F C D+V+VE + K C+ D C+C+ C C + +Y +C + WRT CP C +V+ CG+ C D+C N C + CI+GCFCP + N C+ C C+ Y +G+ C EC C G + C
Sbjct: 23 EHNGQ---VCSTWGNYHFKTYDGDVFQLPSTCNHILTSQ-CRGSYEAF-----NIQLRRQVENGEPTISKITMKLDGTVV---------DVKAPHLGSGVFIET--ISSYIMVKATVGLTAMWNEDDALMIELNAKFINQTCGLCGDFNGVPTYNEFIKDG-AQLSATDYGNLWKMDG-------PTETCEEQPER---VNENCGDENFCQNLFLSKAFSSCTDLVSVEPFTKVCIEDLCQCNSSAGSSCLCDTLAEYSRQCVHAGGQPANWRTEHFCPMKCPESKVYMECGSPCMDTC----SNPERGQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCIH--GGKAYASGEGYSSSCRECTCSGGQWNC 360
BLAST of von Willebrand factor vs. Ensembl Cavefish
Match: ENSAMXT00000019204.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001825.1:10421:34530:-1 gene:ENSAMXG00000018303.2 transcript:ENSAMXT00000019204.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 441.425 bits (1134), Expect = 1.852e-128 Identity = 331/1150 (28.78%), Postives = 510/1150 (44.35%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACT-LKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM---CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGV--EILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCP-NENMKYNQCGSRCTSTCRLLQESKCKEE--CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDC--------GI-CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP--VPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC 3832
CS+ H KTFDG+V+ + Y L C G+ F V+ S D K + +T+G I L +V ++ K+P G+++ ++IK+ L +++++D + V + CGLCG FNG+ +N +I E C+ Y C +++++ ++C S V + Y+ C + C E+S C +YS C+ G +WR C +C E+SE GS C C L CE + GCYCP +D C+PQE+C C + Y G L + + C+C HG W C E+ G C + H+ TFD + F F G C Y +L + + ++ Q C D+ C +++ V++ ++ A+ + +K N ++LPY + +++ + S+ + +++ G+ ++ ++ I L + Y+ K G+CGNFN D+ T +G+ E F SWK C DR C+ + YA C KL E E F KCHS+ N + ++ +C + CTC N C C FSSY R C + I L + +C K CP + Y G + T L+ C ++GC CP+G ++ CV + C C +G P + + +C CTNG C S + CP +F+C G+ C KTC + C C GC CP + G C+ CPC N Y I C N C CK GKW C + K C G C +G HY +FD + F F G C Y+ +++ CGNK GTF +I+EN+ CG++ ++C+KS++ + + G NT+ G Y +V + + G+ V WD+ ++ I++ ++ +VCGLCGNF+ +D + D++ FA+SWKV+S CP VP P CSK +R WAK +C I+ +FK+CH +V+ Y+ CV D+C CD G DC CFCTAV Y CNE + + WRT E CP C
Sbjct: 4 CSMWGNFHFKTFDGDVYQFQGICEYNLVSHCHGSVQDFSVHVKRSEFTGDSKINR-----VLVTIGDMAIKL--TKNLVMVNDDVVKLPHYVAGVLLEQYATYIKLYAKLGLSVMWNKDDAVMVELEAKYTNQTCGLCGDFNGM-----------------AILNEFI------------SEAQACD---------YSSQRPGCSELLEDKAWKSCTSIVSPEPYIQACVVDRCWSRPGDIEDSL------PLCTTLSEYSRQCSHAGGTPPNWRKPD-FCAV-KCPYNMEYSESGSPCVDTCSHTDTSLLCEEHKMDGCYCPPGTVFDDISDRGCIPQEQCQCKHNK---VYNSGDVLHMEDKECVC-HHGSWTC-ESLPTPGLCAVEEGSHFTTFDGKEFTFHGECYY---------ILSQDCEESKFTVLGQLIPCVDHETDTCLKSV-VLLPNNDKNNALVIKDDGTVKQNG-DLTLPY---ASANIIVFRPSSFHIIVQTSFGLRMQVQLVPVMQLYITLDRSYQTKTCGLCGNFNMVLSDELKTPQGLVEGTASSFANSWKAQSNCR-DRS-ESLDDPCSYNIDSESYAEHWCSKLLEKEGPFAKCHSSVNPESYYKRCKYSTCTCEKTEN-----CLCGVFSSYVRACTAKGIFLQGWRDTVCKKYTEHCPATQTFSYKLQGCQRTCDSLSLERPACINTYLPVDGCACPDG-LYENENGVCVALDKCSCYHNGERIKPGKSITIKNEHCVCTNGKLHCHSWKPSVMD-CPAPKVFFNCSEAGPEEHGLECAKTCAQKTVDCFLTGCT-SGCQCPSGLLDDGRGHCVPQHNCPCKHNGHYYPPGSQITD---QC-NTCTCKQGKWKCTEEK-CPGTCTIYGSGHYNTFDNRRFGFSGDCGYIAAQNKCGNKTGTFHVITENVPCGTTGTTCSKSVRIVLGKTQLDLIDGK----VTNTDLE-SGTLIKYRVRNVGLYLAIEADIGLTVLWDRKTTVRIILQPQHMGEVCGLCGNFNGNGKDDFTTQGQLQVSDVLEFANSWKVNSHCPDAVPDFDP--CSKAHNRHTWAKMQCSIITGKTFKECHSMVDPAPYFDNCVRDSCACDTGGDCECFCTAVAAYAQACNEAGVCVAWRTPEICPVFC 1059 HSP 2 Score: 101.679 bits (252), Expect = 6.309e-21 Identity = 91/378 (24.07%), Postives = 159/378 (42.06%), Query Frame = 2
Query: 3035 ICKTWGETHYESFDGKYFEFQGSCSYVIS-------EDFC-----GNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFD-LRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGD--DCVCFCTAVEQYVARCNEY-EIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW---QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
+C WG H+++FDG ++FQG C Y + +DF G K+ + G + TK++ + N V + +Y G Y T+ L+ G+ V W+K ++ + + KY N+ CGLCG+F+ + I N+ + + + S+RP E+L+ ++K C +V+ E Y + CV D C GD D + CT + +Y +C+ WR + C C + G+ C D+C ++ C++ ++GC+CP G + + C+ ++ C+C N YN+GD + E +EC C+ G++ C L
Sbjct: 3 VCSMWGNFHFKTFDGDVYQFQGICEYNLVSHCHGSVQDFSVHVKRSEFTGDSKINRVLVTIGDMAIKLTKNLVMV------------NDDVVKLPHYVAGVLLEQY-----ATYIKLYAKLGLSVMWNKDDAVMVELEAKYTNQTCGLCGDFNGMAILNEFISEAQACDYS------------------------SQRP-------GCSELLEDKAWKSCTSIVSPEPYIQACVVDRCWSRPGDIEDSLPLCTTLSEYSRQCSHAGGTPPNWRKPDFCAVKCPYNMEYSESGSPCVDTCSHTDTSLL----CEEHKMDGCYCPPGTVFDDISDRGCIPQEQCQCKH---NKVYNSGDVLHMEDKECVCHHGSWTCESL 325
BLAST of von Willebrand factor vs. Ensembl Cavefish
Match: otog (otogelin [Source:ZFIN;Acc:ZDB-GENE-120228-1]) HSP 1 Score: 444.121 bits (1141), Expect = 1.995e-126 Identity = 333/1229 (27.10%), Postives = 547/1229 (44.51%), Query Frame = 2
Query: 242 SLGWQGNRCSDPICVGGCSNGGKCVAPNQCLC-LDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDC---LGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEI-KVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTN-ELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG------------ICQKTCTN--LDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFV------RGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS--SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC 3832
+L W+G R P C C NGG+CV P+ C C L +G +C T V N+ C ++ +TFDG + +P + YTL DC + + L V+ + F C+ S+ + + + I ++ K K+P N + + + ++ + + + + +IY+ + CGLCG FN ++DD + V +++ F NSW++ +P YP + +++ K +C ++K ++C V Y+ C +++C + P + C++ +Y+ AC ++ WR+ +C +C ++ EC C +C M ++ + QCL GCYC + ++ +CV CPC ++ Y G+ ++ C C C G W C E ++C G C ++GD ++ +FD R + F C+Y VL + ++TIQN C N + C +++ V++ ED + + L S E+ + +ISLPY + I ++S+ +++K+ G+ + +N R+ ++L + +R+ G+CG FNGN DD + G+ E F +W+ + C R + C + + YA + C L + E F CH + F QC DVC CG C C A + YAR C N+ + +R++ C CP M+Y C S C C C +EC EGC CP+G + CVK ++C C F G Y P ++ C +G + S + + CP + DCG C +TC + L+L C + +PGC CP + H EC +P CPC K Y D + + C C+C+ G + CE + C +C +G+ HY +FDG F++ G+C + + + + +ENI C S C K++ I + R F + ++ R W++ T L E + V WD+ ++ I ++Q K+ GLCGNFDL+ N+MR F +SW + P + P CS P RE +AK +C IL S+ F+ CH VV+V +Y C+AD C C RG DC CFC++V Y RC + + WR+ CP+ C
Sbjct: 78 ALRWEGRRARGP-CTFPCLNGGQCVYPDSCNCSLYQATGTHCQT------------------VPNSGLEREMTCRSWGQYNYETFDGLYYYFPGKCSYTLLKDCEENIQPSLLIQVHNDPECTSFPYS----CTRSVSLFLPWEGEIRL-QDLNVTFKGQSVKLPHNIHDLELEQIADYVLVSQPQVFTLAWHGLNGSIYIKMSPEFVGRTCGLCGNFNADVNDDLKTSYGVFTEDLAMFGNSWVEEEPQKATCSMIPYLYP-SPCSGQDSHVLLKVEEVCATLLKEPF-KSCHEFVSPYSYMASCSNDLC---LSGPNGDVV-------CQVFTEYARACAHADHPLNKWRTHVPVCGV-ECPAELQYRECIPCCPVHCRTEQMCIDSKLQCLDGCYCQDDLIYEDGLCVKASDCPC--EHRGMLYPSGHTIQEECNNCTCS-GGAWNCTE-QKCPGECSVTGDMYFQSFDGRVYTFLATCQY---------VLAKSRNSGGFTVTIQNAPCGPNLDGACIQSVNVVLNEDP-RTEVTLSHSGEVFMASQFRISLPY---SDDVFQIQELSSLFVQVKTSQGLLLQYNWREFRLYLQLGELWRDDTVGLCGTFNGNIQDDFLAPSGMIESTPQLFGNAWRVSSAC-VPRPSLPQLDPCDTHQQAASYAVEKCEVLMQ-EVFAVCHEHVSPVGFQLQCRADVCKCGTP-------CLCSALAHYARTCRKHNVIIEFRSHVPECSVACP-ATMEYGLCVSSCQRRCLSFSMPQSCGDECEEGCVCPQGTYYSTHTHSCVKRNECPCSFLGTDYSPGDVIMTSSGVQICRDGQIVLQSTITD--KLCPPGQIYEDCGNMVDGPSVSKGLACDRTCESYLLNLTCSTHEPCVPGCVCPTGLLKHGDECFEPVACPCLWKGKEYYPGDKVS---STCHL-CVCQQGTFQCE-FRPCPSMCTAYGDRHYRTFDGLLFDYVGACKVYLLKSSADVMVS---VTTENIDCFESGVICRKAL-LISVGRSFIVFDDETGKPSPSSVIDRQN--------VLIWSAGYFTIVHLSQYE-LSVLWDRKTTIHIQAGPRWQGKLSGLCGNFDLKTVNEMRTPDNMDSATPQEFGNSWTAAECVNSPDIRHP---CSLNPLREAFAKKQCGILLSEVFQVCHPVVDVTWFYMNCLADTCGCSRGGDCECFCSSVAAYAHRCCHQGVTVDWRSPSLCPYDC 1219 HSP 2 Score: 156.762 bits (395), Expect = 1.443e-37 Identity = 120/461 (26.03%), Postives = 206/461 (44.69%), Query Frame = 2
Query: 2747 CPKSMAWFDCGICQKTCTNLDLVCPKGQCQ-LPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXX-XCTKSIKFIY----LNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI-LFTMEGIQVKWD-KAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPF-MCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTN-ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW--QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEG 4090
CP + + +C C + +C + Q L GC+C ++ + +G C+K +CPC +Y TI C N C C G W C + K C G C G+ +++SFDG+ + F +C YV+++ G F + +N CG + +C +S+ + V G + S+ P F ++ + F+ + T +G+ ++++ + L + + E +++ GLCG F+ I +D A SG IE F ++W+V S+C P P P C +A KCE+L + F CH+ V+ + +C AD C+C C C+A+ Y C ++ + +++R++ C C + C + C C+ C C EGC CP G ++ + CV+R C C Y+ GD ++ C +G
Sbjct: 403 CPAELQYRECIPCCPVHCRTEQMCIDSKLQCLDGCYCQDDLIYEDGLCVKASDCPCEHRGMLYPSGHTIQE---ECNN-CTCSGGAWNCTEQK-CPGECSVTGDMYFQSFDGRVYTFLATCQYVLAK---SRNSGGFTVTIQNAPCGPNLDGACIQSVNVVLNEDPRTEVTLSHSGEVFMASQFRISLPYSDDVFQ-IQELSSLFVQVKTSQGLLLQYNWREFRLYLQLGELWRDDTVGLCGTFNGNIQDDFLAPSGMIESTPQLFGNAWRVSSACVPRPSLPQLDPCDTHQQAASYAVEKCEVLMQEVFAVCHEHVSPVGFQLQCRADVCKCGTP----CLCSALAHYARTCRKHNVIIEFRSHVPECSVACPATMEYGLCVSSCQRRCLSFSMP----QSCGDECEEGCVCPQGTYYSTHTHSCVKRNECPCS--FLGTDYSPGDVIMTSSGVQICRDG 844 HSP 3 Score: 118.627 bits (296), Expect = 5.834e-26 Identity = 112/438 (25.57%), Postives = 181/438 (41.32%), Query Frame = 2
Query: 2870 LHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLIS--ENIACGXXXXXCTKSIKF-------IYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKA-MSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSW----KVHSSCP-VPQKPPFMCSKRPDREPWAKAK--CEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTN-ENCPHTCTGKRVWKNCGNICP------DSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTK 4108
L+ G+C+ P C C +Y T +CQ V + C++WG+ +YE+FDG Y+ F G CSY + +D C + LI + C S SCT+S+ I L + +G + + N + + + ++ + + W S+ I + ++ + CGLCGNF+ +++D++ G +D+ F +SW ++C +P P CS + D K + C L + FK CH+ V+ Y C D C D VC +Y C + L KWRT+ C C + ++ C CP CID K C++GC+C D L +++ CV+ C C + Y +G + EC C C GA+ CT+
Sbjct: 95 LNGGQCVYPDSCNC----SLYQATGT------HCQT---------VPNSGLEREMTCRSWGQYNYETFDGLYYYFPGKCSYTLLKD-CEENIQPSLLIQVHNDPECTSFPYSCTRSVSLFLPWEGEIRLQDLNVTFKGQSVKLPHNIHD--------LELEQIADYVLVSQPQVFTLAWHGLNGSIYIKMSPEFVGRTCGLCGNFNADVNDDLKTSYGVFTEDLAMFGNSWVEEEPQKATCSMIPYLYPSPCSGQ-DSHVLLKVEEVCATLLKEPFKSCHEFVSPYSYMASCSNDLCLSGPNGDVVC--QVFTEYARACAHADHPLNKWRTHVPVCGVECPAELQYRECIPCCPVHCRTEQMCID----------SKLQCLDGCYCQDDLIYEDGLCVKASDCPCEH--RGMLYPSGHTIQEECNNCTCSGGAWNCTE 489 HSP 4 Score: 68.9366 bits (167), Expect = 6.751e-11 Identity = 60/242 (24.79%), Postives = 102/242 (42.15%), Query Frame = 2
Query: 3326 DVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVC--GLCGNFDLRIDNDMRARSGEI---EKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCE----ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDG--LHWQNNE-CVERKACECVD 4015
D +++ + G++++W + + ++ E Y +K+ GLCG D ND+ +G I +D F DSW+V P ++ R + + C +L + +F CH V + + V DA + C A+ YVA C+++ I ++WR+ + CP C ++ C C + D C + EGC CP G LH + C+ C C D
Sbjct: 2466 DTGNMYLIRSPAGLKIQWFHSTGMMVIETETYTHKLSTMGLCGCCDGNPLNDLTLSNGSIMAESEDPAVFIDSWQV------PNTTSYISHSRRREVNCSTSDCSACLNMLNNLTFTACHPFVPPATFCEVWVRDAEYVNNP------CVALAAYVASCHKFNICIEWRSPDYCPFICPESLRYQACLPACTAPSCPNQEFEYDAGQCSGLA-EGCVCPQGTLLHRPYSALCIPPNKCACTD 2694 HSP 5 Score: 67.3958 bits (163), Expect = 2.048e-10 Identity = 86/374 (22.99%), Postives = 145/374 (38.77%), Query Frame = 2
Query: 1568 QCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTI---GVIIGEDEDQVAINLL------SSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVT--GMCGNFNGNEMDDSMTFKG---VDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQE-----SKCKEECIEGCFCPEGETFDEA-AKECVKISDCKC 2629
+C +C RC + D + +TFD + Y L N T+ SI +Q N DN+ + + I + +QV IN L ++ K F K TGN + ++S G++I W + + + ++ Y K++ G+CG +GN ++D G + +D F+ SW+ T + + ++ S SD + C + + F CH F + R+ + C A ++Y C NI + WR+ + CP CP E+++Y C CT+ QE +C EGC CP+G + C+ + C C
Sbjct: 2357 KCCPKWDCPCRCSVFPDLNVVTFDGNNVAIFKAASYVVTQ-------LPNETV---SILVQECNSSDNTLVWNFTNLCLAALNITHESNQVLINRLQRRLYVNTRYAKPRFKKYGFEILDTGN-----------MYLIRSPAGLKIQWFHSTGMMVIETETYTHKLSTMGLCGCCDGNPLNDLTLSNGSIMAESEDPAVFIDSWQVPNT----TSYISHSRRREVNCSTSDCS--ACLNMLNNLTFTACHPFVPPATFCEVWV---------RDAEYVNNPCVALAAYVASCHKFNICIEWRSPDYCPFICP-ESLRYQACLPACTAPSCPNQEFEYDAGQCS-GLAEGCVCPQGTLLHRPYSALCIPPNKCAC 2692
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008773.1 (pep scaffold:Pmarinus_7.0:GL476328:703641:749596:1 gene:ENSPMAG00000007891.1 transcript:ENSPMAT00000008773.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 474.167 bits (1219), Expect = 7.375e-138 Identity = 345/1181 (29.21%), Postives = 551/1181 (46.66%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGID-DLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTIC-KQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTM---CVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS--KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPK---KCPNENMKYNQCGSRCTSTCRLLQE--SKCKEE--CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFY-LPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG---------ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFC--GNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAF--YWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRV-------WKNCGNICPDSCID 3889
CS +H KTFDG F + Y +C A P F V +++ N+ I I + I + E A V +PFN GI + ++G +KI + ++ +++ ++ + + K CGLCG +NG +Y +F I + +N+ DP + + EQ P D +LC+ ++ C++ + + Y+ C + C C P+ T+E YS C+ ++ G +WR+ + C P+C G + ECG C +C Q + C +C+PGC+CPR WD C+P E+C CV + + TY G L +C+ C+C G W C E + C C + G H TFD+ F F G C Y M ++ ++ ++ C+ ++ C +++ +I+G + ++ I+ S E+ VN V+++LPY + + I S+ + L + GV +L +++ L+ + G+CG++NG + DD T G+ E F SWK TC E C LS ++ YA +C + + F CH + + F+ +C +D C+C + C C A SSYA CA +++ L LC K CP + Y+ C +TCR+LQE S+C ++GC CP+G T+D C+ +C C + G L ++Q + C C G +C + + CP M +F+C C +TC+ + P+ GC CP+ V + G CI+P CPC N + + ++I +C N C C + KW C N C C +G+ HY +FDG+ F F+GSC+YVI++D C N GTF + +EN+ CG++ ++C++SI N V + + S I ++ GP + + ++ T I + WDK + I + +Y+ VCGLCGN D R ND+ R+ + +D + F +SWK+ C ++ C+ R PWA+ +C ILKSD+F CH +V+V +++ CV DAC CD G DC C C AV Y CNE +KWRT + CP C ++ CG CP +C D
Sbjct: 1 CSTWGQNHFKTFDGKFFDLGISCNYLFASNCKDATPNFNVII--------RRSVNNPQIISSINISIIDINIDLSAEGAAVNKESQDLPFNQYGIQIIALGDDVKITATNIGFKLFWNKKDSLLLQVTESYRNKTCGLCGDYNG--DAEYPEFKGGILSVAEYALNNKY-YDP--MVQCDQIEQPP-------TPDDCSMHESLCELVLNTPEFADCKARLDVRPYIGACIVDQCSCTSDCPDFGTVEE-YSRECR---------SIGGTPGNWRNTSETCGAAPECLPGTIYQECGIPCPNSCSQPSLSNMCIEECVPGCFCPRGTIWDDLTDKGCIPLEECSCVFN--KHTYNAGDILHQDCQDCVC-HGGVWNC-EEQPCTRTCALEGGSHIKTFDQNQFTFHGDCYYFMAMDCRTHLF---------NLMVKLKPCE--TKNTCLKSLALILGRNVLKIMIS--DSGEVSVNDVEVNLPYD---SNAITIFYQSSFYIHLHTSIGVNLLVQLSPVMQLYLTLDPSFHTATCGLCGDYNGIQSDDFKTSNGLIEGTAIYFANSWKAQNTCPPPTESTEEP--CMLSLNHKQYAEDMCSNMKTPDGPFSACHGTVSPEAFYKRCLYDTCSCQDS-----EACLCAALSSYALACASKHVVLEGWREHLCAKYTENCPGSQV-YSYNALPCVNTCRVLQETGSQCFSSGVAVDGCTCPKG-TYDSGGGFCMAPEECPCYYRGNVLPLGNSVQQGK-YICECRAGTMLCMNPQGPTPQPCPHPMVYFNCNQSTANGNSFPCHQTCSLQECYNPECN---SGCVCPKGLVYSDDGTCIQPEACPCEHNGQKTAPGESI---EVDC-NTCTCVNRKWECTNNT-CLSTCSLYGQGHYRTFDGQRFLFEGSCTYVIAQDNCQNPNNEGTFNITTENVPCGTTGTTCSRSINVQVGNWVIKLFQKSYSI-----DFLGSGPETINPFQIKHRGLYLVVETSNRITLTWDKNTGVFISLGPEYKGTVCGLCGNHDGRAANDLTTRTHMVVEDPLEFGNSWKLSGLCADAKETSSPCALMSHRLPWAQQRCGILKSDTFASCHALVDVLPFHEACVHDACGCDTGGDCECLCNAVAVYAKACNEAGQCIKWRTPDLCPLFCEYYDAPDDCTWHYRPCGQSCPKTCRD 1108 HSP 2 Score: 113.235 bits (282), Expect = 4.232e-25 Identity = 101/371 (27.22%), Postives = 163/371 (43.94%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADS------WKVHSSCPVPQKPPFMCSKRPDREPWAKAKCE-ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL---KWR-TNENCPHT--CTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQN---NECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC 4102
C TWG+ H+++FDGK+F+ SC+Y+ + + C + F +I S I + + + V++ + P + D I T G ++ W+K SL + + E Y+NK CGLCG+++ A E + I++ A+ + C ++PP PD ++ CE +L + F C ++V Y C+ D C C DC F T VE+Y C IG WR T+E C C +++ CG CP+SC + + C + C+ GCFCP G W + C+ + C CV YN GD + ++C++C C+ G + C
Sbjct: 1 CSTWGQNHFKTFDGKFFDLGISCNYLFASN-CKDATPNFNVIIRRSVNNPQ----IISSINISIIDINIDLSAEGAAVNKESQDLPFNQYGIQIIALGDDVKITATNIGFKLFWNKKDSLLLQVTESYRNKTCGLCGDYN------GDAEYPEFKGGILSVAEYALNNKYYDPMVQCDQIEQPP-----TPDDCSMHESLCELVLNTPEFADCKARLDVRPYIGACIVDQCSCT--SDCPDFGT-VEEYSRECRS--IGGTPGNWRNTSETCGAAPECLPGTIYQECGIPCPNSC----SQPSLSNMCIEECVPGCFCPRGTIWDDLTDKGCIPLEECSCV--FNKHTYNAGDILHQDCQDCVCHGGVWNC 344 HSP 3 Score: 110.923 bits (276), Expect = 1.945e-24 Identity = 117/427 (27.40%), Postives = 177/427 (41.45%), Query Frame = 2
Query: 3023 QCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENI-ACGXXXXXCTKSIKFIY--LNRVFQFVRGSNPI--------VSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEK-YQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQ-KPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSC---------IDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNEC-VERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPP---HCVNEYEFLCPNNFCARR 4219
+C IC WG+ HY +FDG Y+ F C+YV+ E L F++ N C +C +SI Y N + + + P V Y G T +S + T + +E + + + I +P K + N G CG +D R R+G ++K VN A WKV P P K S PD C ++ SD F +C V++ + + C D C R DD C +++ Y ++C + I + WR TN C +TC +V+ CG P +C + KN + EGCFCP+G N++ + +C C P GD+ I C +C C E + S C++ S P + KPP +N E P++ C R
Sbjct: 1327 ECECICSAWGDPHYTTFDGTYYTFTDDCTYVLVEQIIP-VLPDFRVTVNNFRGCSGGGPACPRSITITYNGTNVIMETSQRKKPTRLFVKFNGVGVTPVYSENGMTVA--SSGISTRVDIPEIEAVITF--EYVRFSITLPYKFFFNNTQGQCGTCTNNRQDDCRMRNGVVDKSCVNMARDWKV----PDPSCKTNPSSSPPPDVTCPPHPLCNLIGSDLFAECKRVIDPQPFVMGCQFDWCA-TRKDDIPCL--SIKAYASQCLDRGICVDWRNFTNNRCSYTCPIGKVYNACGPSIPPTCDTRLVWQSYLCYYKNFVYWLTMED---EGCFCPEGTILHNSQTDICTTSCGCTGP-NGYPKQYGDRWISNCLDCVCDEHS------SSVVCVNHSCPPVP-KPPCQGQGMNLVEVQDPSDACCTR 1730 HSP 4 Score: 82.8037 bits (203), Expect = 7.542e-16 Identity = 104/409 (25.43%), Postives = 158/409 (38.63%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTI-------GVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVST--DILRLKSETGVEILWNGGSRIEIKLSKK-YREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKT-NPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTC--RLLQESK-CKEECI--------EGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQ-DRCNNCTCT--NGNWICTSNDC 2731
ECE C GDPHY TFD ++ F C Y V + ++ + +T+ N CPR+I VI+ + + L +K N V ++ Y G T V +ST DI +++ E + R I L K + G CG N DD GV +K WK +P+C+++ + + + + +C+ L S+ F +C + F C D C D C + +YA QC I + WR TN C CP + YN CG TC RL+ +S C + EGCFCPEG + + + + C C + Y R C +C C + + +C ++ C
Sbjct: 1327 ECECICSAWGDPHYTTFDGTYYTFTDDCTYVLV----EQII---PVLPDFRVTVNNFRGCSGGGPACPRSITITYNGTNVIMETSQRKKPTRLF----VKFNGVGVTPVYSENGMT-VASSGISTRVDIPEIEAVITFEYV-----RFSITLPYKFFFNNTQGQCGTCTNNRQDDCRMRNGVVDKSCVNMARDWKVPDPSCKTNP-------SSSPPPDVTCPPHPLCN-LIGSDLFAECKRVIDPQPFVMGCQFDWCA------TRKDDIPCLSIKAYASQCLDRGICVDWRNFTNNRCSYTCPIGKV-YNACGPSIPPTCDTRLVWQSYLCYYKNFVYWLTMEDEGCFCPEGTILHNSQTD-ICTTSCGCTGPNGYPKQYGDRWISNCLDCVCDEHSSSVVCVNHSC 1702 HSP 5 Score: 72.0182 bits (175), Expect = 1.459e-12 Identity = 102/446 (22.87%), Postives = 173/446 (38.79%), Query Frame = 2
Query: 236 SCSLGWQGNRCSDPICVGGC--------SNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTR----VNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFD-QKTQNDCSISMKITVGTQTIILFPKNEFA--LVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQE-----FSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCVPQEKCPCVRDNGES 1498
+CSL C +P C GC S+ G C+ P C C N T +S + D + V R NNT ST CS+ H +TFDG F + Y + D P FN + + T CS S+ + VG I LF K+ L +T PF + G ++ ++ + + + +D++ +++ + +CGLCG +G ++D ++ ++ F NSW S L + PC + + Q+ ++K++ +C + V + + + C + C C T +C C Y+ AC G + WR+ + ++ + + CG +C K C E Q GCY C ++ + D +CV + C ++G S
Sbjct: 744 TCSL----QECYNPECNSGCVCPKGLVYSDDGTCIQPEACPCEHNGQ----KTAPGESIEVDCNTCTCVNRKWECTNNTCLST---CSLYGQGHYRTFDGQRFLFEGSCTYVIAQDNC-QNPNNEGTFNITTENVPCGTTGTTCSRSINVQVGNWVIKLFQKSYSIDFLGSGPETINPFQ-----IKHRGLYLVVETSNRITLTWDKNTGVFISLGPEYKGTVCGLCGNHDGRAANDLTTRTHMVVEDPLEFGNSW---KLSGLCADAKETSSPCALMSHRLPWAQQRC-----GILKSDTFASCHALVDVLPFHEACVHDACGC-------DTGGDC-ECLCNAVAVYAKACNEAGQCIKWRTPDLCPLFCEYYDAPDDCTWHYRPCGQSCPKTCRDPHAECREGVQRFEGCYPKCTESKPYFDEDNMLCVSWDSCGTCSESGIS 1156 HSP 6 Score: 52.7582 bits (125), Expect = 8.668e-7 Identity = 95/407 (23.34%), Postives = 137/407 (33.66%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIIL----FPKNEFALVKNNKTKI-PFNSE-GIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKIC-----GLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQ-----------------LGMELECESQCLPGCYCPRNRYWDGTMC-VPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEEC 1591
CS H TFDG +++ Y L + P F V N C C S+ IT +I+ K VK N + P SE G+ V+S G ++ I ++E + + YV +T K G CG DD R N V+ K+ W DPS N D LC +++ ++L C+ + ++ CQ + C + C + Y+ C +GI VDWR+ T C G+ ++ CG + C L ME E GC+CP + + C C NG Q G R NC C+C H N C
Sbjct: 1332 CSAWGDPHYTTFDGTYYTFTDDCTYVLVEQIIPVLPDFRVTVNNFRGCSGGGPA--CPRSITITYNGTNVIMETSQRKKPTRLFVKFNGVGVTPVYSENGMTVASSGISTRVD-IPEIEAVITFE---YVRFSITLPYKFFFNNTQGQCGTCTNNRQDDCRMRNGVVDKSCVNMARDWKVPDPSCKT----------NPSSSPPPDVTCPPHPLC-NLIGSDLFAECKRVIDPQPFVMGCQFDWCATRKDD-----------IPCLSIKAYASQCLDRGICVDWRNFTNNRCSYTCPIGKVYNACGPSIPPTCDTRLVWQSYLCYYKNFVYWLTMEDE-------GCFCPEGTILHNSQTDICTTSCGCTGPNGYPK-QYGDRWISNCLDCVCDEHSSSVVCVNHSC 1702
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010519.1 (pep scaffold:Pmarinus_7.0:GL477612:23664:47127:1 gene:ENSPMAG00000009527.1 transcript:ENSPMAT00000010519.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 412.149 bits (1058), Expect = 2.120e-121 Identity = 354/1248 (28.37%), Postives = 532/1248 (42.63%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNR---YWD---GTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVE--ILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTN--ELCPKKCP-NENMKYN--QCGSRCTST----CRLLQESKCK---EECIEGCFCPEGETFDEAAKECVKISDCKC--EFHGRFYLPYEIRQDRCNNCTCTNGNWIC------TSNDCEGKEFCPKSMAWFDC--------GICQKTCTNLDLVCPKGQCQLPGCFCPENTVL-HNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWK-VHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC------TGKRVW--KNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACECVDPITNLKYN 4039
CSV H+ TFD PL L + + +P + F Q++ D + V + I L E + + ++P+ I + VGS+IK I +L+D++ I V K GLCG FNGI D Y N + +V D + E E C ++ D K+L +V C+ + L C C ++S CT + WRS C C GQ ECGS C C + C S C+ GC+CP WD G+ C+PQ C C G+S Y G L C+ C C G+W C ++ C G C I G ++ TFD + + F G C YT + N S+ + T C+ C ++ I + + +S ++ V+ K+ LP+ + V I Q S+ LR ++ G+ + ++ I + Y K G+CGNFN DD T G+ + F WK TC DR V C++ + ++YA C L +++ F CHS +AD+++ +C +D C+C + C C A S+YA C+ + L WR++ L + CP + Y +CGS T CR ++ + +EGC C G +D+ CV SDC C E G C CT G C + N CEG M + +C CQ+TC N +C C + GC CP ++ HNG C+ +C C N + Y DTI + N C C + +W C ++ C G+C +GE H+ +FDGK + F GSC Y++S+D CGN G+F++ +EN CG++ ++C+K + F G+ V ++ PG P + + + ++ T G+ + WD ++ + + + + +VCGLCG++D ++ ND R+G + FA WK S CP + C+ RE WA +C +LK FK+CH++V YY CV D+C CD G DC C CTA+ Y C + L+WRT + CP C G+ W + CG C SC + T N +EGC+ CP + N CVER C+CV T K N
Sbjct: 2 CSVWGTSHLLTFDHASMDLPLSS-CALNLVSITDSPSLSLSF--------QRSALDRMSVENVVVTMEGISLSLTREGQSISGQRVQLPYKDHSIQIEMVGSYIKCSSIMGFSLLWDQNNAITVKLDPKFNTKTTGLCGDFNGIADDFY--LNVMSTTSVLFSFLLTCDEPTQHIEHLFEDELLSCVSVM----DHQLKSL-----LVNILWSDICRLDCNGTAGLYPCDG----------------------CATIAEFSRRCTQANESPGTWRSPE-FCGV-TCPRGQVHRECGSVCKDTCSNPSASVNCPSTCVDGCFCPEGTAGTVWDDVSGSGCIPQSNCGC-NQLGQS-YASGESLITGCKNCTC-EQGQWNC-DSRPCYGTCKILGGSYFSTFDSKTYRFHGDCFYTLTTDCAGN---------KFSVLGELTKCEGRDSKTCLNSVIFYI---DGIRTVVFKASGDVLVHENKVPLPFV---SASVTIVQESSFYLRAETNFGLRLRVQLKPLMQLYITAATAYHNKTCGLCGNFNEVTGDD-FTHSGIVDATAATFCNVWKAQATCP-DREEVTMD-PCSVYITNNEYAKAHCGLLMDNDGPFSTCHSVVSADEYYTRCKYDTCSCSYP-----EPCLCAALSAYAYACSAHGVVLVGWRSDICSLDAENCPVSHEFSYAAPECGSASCRTSREVCRGVRPPPFELPSPSPVEGCVCGAG-LYDDEHGVCVLKSDCPCYSEMSGDLIRAGVTVDLHDTQCLCTGGELHCFGLSPISGNTCEGL------MTYHNCSDGSSEGSAACQRTCANPHSICYSPGC-VSGCVCPGGLIVDHNGNCVSEEQCTCEHNGRHYDPGDTITQ----LCNKCTCMNARWSC-TSEVCQGLCFVYGEGHHTTFDGKQYVFDGSCQYILSQDSCGNSSGSFRITTENEPCGTTGTTCSKKVHFYAGADELVLTDGT---VKGSSLDFPGSP---FHVRRMGIYTVVETSLGVTIIWDGHTAVSVHVNPQLKGRVCGLCGDYDGKVANDFVTRTGSVAASAPEFAAGWKESGSGCPDLSRVSDPCTINHHRESWAHKQCSVLKDGVFKQCHNMVEYLPYYAACVRDSCGCDTGGDCHCLCTALASYAQACGSAGVCLRWRTPDLCPLGCDLYNKDEGQCEWHYRECGRGCQRSCTNRNAICTANL----PPLEGCYPTCPSDRPFLNESSMTCVER--CDCVSNGTTYKVN 1153 HSP 2 Score: 161.77 bits (408), Expect = 3.882e-40 Identity = 139/486 (28.60%), Postives = 217/486 (44.65%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTN--LDLVCPKGQCQLPGCFCPENTV------LHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIAC-GXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFIL-----FTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP----VPQKPPFMCSKRPDREPWAKAKCEILKSD--SFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTN------ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTG--CKKVCIEGCFCPDGLHW-QNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
CP+ +CG +C+ TC+N + CP C + GCFCPE T + CI C C + Y+ ++++ G C+N C C+ G+W C+ ++ C G CK G +++ +FD K + F G C Y ++ D GNK F ++ E C G S +C S+ F Y++ + V ++ V + N P + T ++ F L F + ++V+ M L I Y NK CGLCGNF+ + D SG ++ F + WK ++CP V P CS +AKA C +L + F CH VV+ + YY RC D C C + C+C A+ Y C+ + + L WR++ ENCP + CG+ + ++ + V +EGC C GL+ ++ CV + C C ++ G V +C C G C L
Sbjct: 243 CPRGQVHRECGSVCKDTCSNPSASVNCPS-TC-VDGCFCPEGTAGTVWDDVSGSGCIPQSNCGCNQLGQSYASGESLITG---CKN-CTCEQGQWNCD-SRPCYGTCKILGGSYFSTFDSKTYRFHGDCFYTLTTDCAGNK---FSVLGELTKCEGRDSKTCLNSVIF-YIDGIRTVVFKASGDVLVHENKVPLPFVSASVTIVQESSFYLRAETNFGLR-LRVQLKPLMQLYITAATAYHNKTCGLCGNFN-EVTGDDFTHSGIVDATAATFCNVWKAQATCPDREEVTMDP---CSVYITNNEYAKAHCGLLMDNDGPFSTCHSVVSADEYYTRCKYDTCSCSYPEPCLC--AALSAYAYACSAHGVVLVGWRSDICSLDAENCPVSHEFSYAAPECGSASCRTSREVCRGVRPPPFELPSPSPVEGCVCGAGLYDDEHGVCVLKSDCPCYSEMSGDLIRAGVTVDLHDTQCLCTGGELHCFGL 710 HSP 3 Score: 99.7525 bits (247), Expect = 4.199e-21 Identity = 100/406 (24.63%), Postives = 150/406 (36.95%), Query Frame = 2
Query: 449 YYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGID---DLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLG--RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIA-VDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLP-----------GCYCPRNRYWD-GTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC-----IENEECEG 1597
Y C ++ G + TFD + + YTL DC G + C + + C S+ + ++F + LV NK +PF S + + SF + L + +Y+ A K CGLCG FN + DD+ + ++ TF N W + + E PC+ N++Y KA ++ +N G TC S V D+Y C+ + C C +P C Y+YAC+ G+ V WRS C EFS CG + E+ C P GC C Y D +CV + CPC + + G + L+ C+C G+ C I CEG
Sbjct: 339 YGTCKILGGSYFSTFDSKTYRFHGDCFYTLTTDCAGNK---FSVLGELTKC-EGRDSKTCLNSVIFYIDGIRTVVFKASGDVLVHENKVPLPFVSASVTIVQESSFYLRAETNFGLRLRVQLKPLMQLYITAATAYHNKTCGLCGNFNEVTGDDFT-HSGIVDATAATFCNVW--KAQATCPDREEVTMDPCSV--YITNNEYAKAHC---GLLMDNDGPFSTCHSVVSADEYYTRCKYDTCSCSYPEP----------CLCAALSAYAYACSAHGVVLVGWRSDICSLDAENCPVSHEFSYAAPECGSASCRTSREV-CRGVRPPPFELPSPSPVEGCVCGAGLYDDEHGVCVLKSDCPCYSEMSGDLIRAGVTVDLHDTQCLC-TGGELHCFGLSPISGNTCEG 720 HSP 4 Score: 72.4034 bits (176), Expect = 1.012e-12 Identity = 99/444 (22.30%), Postives = 166/444 (37.39%), Query Frame = 2
Query: 266 CSDPICVGGC--------SNGGKCVAPNQCLCLDN---YSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQE------FSECGSNCGKNCGQLGMELECESQCLPGCY--CPRNRYW---DGTMCVPQEKCPCVRDNGESTYQQGYRLKLN 1531
C P CV GC + G CV+ QC C N Y + T L + + + V + Y E GHH TFDG + + Y L D G + + + C T CS + G ++L VK + P V +G + ++ + I++D + V+ + ++CGLCG ++G +++D+ + + F W + ++ R + PC N+ + A C ++K+ + + C + V+ Y C + C C T +C+ C Y+ AC G+ + WR+ +N E + ECG C ++C L GCY CP +R + CV E+C CV + +TY+ R+ N
Sbjct: 749 CYSPGCVSGCVCPGGLIVDHNGNCVSEEQCTCEHNGRHYDPGDTITQLCNKCTCMNARWSCTSEVCQGLCFVYGE-----GHH-TTFDGKQYVFDGSCQYILSQDSCGNSSGSFRITTENEPC--GTTGTTCSKKVHFYAGADELVLTD----GTVKGSSLDFP--GSPFHVRRMGIYTVVETSLGVTIIWDGHTAVSVHVNPQLKGRVCGLCGDYDGKVANDFVTRTGSVAASAPEFAAGWKESGSGCPDLSRVSD--PCTI----NHHRESWAHKQCS-VLKDGVFKQCHNMVEYLPYYAACVRDSCGC-------DTGGDCH-CLCTALASYAQACGSAGVCLRWRTPDLCPLGCDLYNKDEGQCEWHYRECGRGCQRSCTNRNAICTANLPPLEGCYPTCPSDRPFLNESSMTCV--ERCDCVSNG--TTYKVNDRIPSN 1159
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008716.1 (pep scaffold:Pmarinus_7.0:GL476328:595486:663084:-1 gene:ENSPMAG00000007851.1 transcript:ENSPMAT00000008716.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 383.645 bits (984), Expect = 4.961e-108 Identity = 354/1270 (27.87%), Postives = 547/1270 (43.07%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISM-KITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEI-LFDRDRNIYVYAPVTAEKKICGLCGYFNG--IMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQY---QKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGT---MCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVI--IGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKL----SKKYREKV---TGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEF-KKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWR-TNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEECI--EGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK--EFCPKSMAWFDCGICQKT--CTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGN--KLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNE---------NCPHTCT------GKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCF--CPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRE-CEECNCYEGAFRC 4102
C+ G KTFD F + Y F DC +SY+ F+ K + + +I T + N N +PF++ I++ + GS +K+ LEI + D V A +++CGLCG F+G ++ Y ++ + ++ + + Q N L QY K+ C +V ++ +C + + Y MC E C+C + + C YS C+L+ + +WRS+ +C C E+ ECGS C C + +C+ C+ GC CP C+ E+C C NG TY ++ ++C +C C G W C C C + H+ TFD R + F G C Y L L Q C + P +G I + + + ++ S+ +I + + +SLPY + V I + S+ +++ + G+ +L +++ + S +RE V TG+CGNFNG DD M+ +G+ E F SWK NP CE D G V + C L+ + + A C L S F K CH + ++ +C D C+C ++ C E F SYA CA + + +WR + +CPN + Y+ + C TCR L + C+E + EGC C EG F CV C C +G P E C C G +C+ + E + P +A + + C L + QCQ PGC CPE + + NG+C+ PC + + + S A+ C G W C + QC CK +G+ HY +FDG+ F+F G+C YV+++DFC + K+GTF++I+ENI CGS S+ C+KSI+F N+ + G I + + G F V + I+ GI++ WD+ SL I+ P QN++CGLCG ++ + +D R + ++F +SWK +C C P R+ W++ KC ILK+D F CH V+ +Y+ CV D+C C+ G DC CFCT+V Y C + WRT + N P +CT G K CGN + ID+G +EGC+ CP + + CV C C + + + G V R+ CE C C G C
Sbjct: 6 CTTWGGGFFKTFDEAFFKFSSNCSYMFFQDC-----------RSSYEEFNIKISHGANEQFSEILFKIDTDVAQFANGKVSFNNRILTLPFSNNKILIQTHGSGVKL-AFRQLEISAYLNDDAFTVVAGDEFRQQLCGLCGNFDGSSTVTTSYSRLKMLLSFSAY------------EVLFFVKCSQLHLNLKTLPAIQQYLIQPKSSGKCTAVVTSSCKGSCG--LNQEGYAAMCAIEKCQC----------GSATTCGCAALSAYSRECSLQYKPLPNWRSSQ-MCPPQDCPGNLEYQECGSTCPPTCANMDPS-KCQEPCVAGCVCPSGTVLSAINEETCIAPEECRC-NYNG-ITYDASDKITIDCNSCTCEA-GSWTCT-TRLCPKACSLESGAHFNTFDSRSYTFHGVCSY---------YLAVKQAHVALRALGQKLECSAKN----PSDLGSISSLTDKKRIRSLEFKSNGKIISSQMSVSLPYI---SDSVKIFRKSSMFIQVDTSYGMMMLVQNSPTMQVYIGLIDSVMFRETVCTPTGLCGNFNGIASDDFMSSQGLVEGTAAAFANSWKVNPDCE-DVGTV-IRDPCQLNTAVAKQAELRCAILRNSTAFFKDCHYLVDPYSYYSRCKQDACSCPSGQD-----CITETFLSYALACASKGLIFENWRKATDSQLVECPNAEV-YSYNANACNQTCRSLSSVDLACQESGLVAEGCQCAEG-LFANDEGMCVPAVSCPCYVNGDILKPGEKVTIADIPCYCREGQLLCSESKKEDHVDPYPPPPVAIQNISTLVRINECVVLYFLQLGRQCQ-PGCICPEGLINNGNGDCVN--NSPCSVCHLVMSGRKEQCHCGASVYK---CYSGSWNCTE-LQCDSTCKMYGDGHYTTFDGQRFQFDGNCEYVVAQDFCVSDPKVGTFQIITENIPCGSMST-CSKSIRFFLQNKEIKLADGKYEIHDVSGSASKGSEEVFT-VHTVGLYLIVKASNGIKLIWDRRTSLFIIAPPDKQNQLCGLCGKYNFDMSDDFTMRDHSLSASPLHFGNSWKASDACSDVTDEASPCEMNPYRKAWSERKCSILKTDVFDACHSQVDHIPFYEACVRDSCACNIGGDCECFCTSVAAYAEACTSAGQCVAWRTPDLCPVFCDYYNSPDSCTWHYRPCGIVNTKTCGN---PAGIDLGNFPQ---------LEGCYPKCPAKTPYLDEHMMTCVSMDKCSC-EMGSQIVPANGTVVNRDTCETCTCVMGNMEC 1186 HSP 2 Score: 107.071 bits (266), Expect = 3.565e-23 Identity = 94/377 (24.93%), Postives = 162/377 (42.97%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRID-----NDMRARSGEIEKDIVNFADSWKVHSSC-PVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCN-EYEIGLKWRTNENCP-HTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDG--LHWQNNE-CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC-TKLFSKEC 4126
+C TWG +++FD +F+F +CSY+ +D C + F NI ++ I F V QF G +R P T +E D A + ++ ++++ ++CGLCGNFD + ++ +++ F ++H + +P ++ +P + KC + + S K + N E Y C + C+C G C C A+ Y C+ +Y+ WR+++ CP C G ++ CG+ CP +C ++ D + C++ C+ GC CP G L N E C+ + C C + Y+ DK+ +C C C G++ C T+L K C
Sbjct: 4 AVCTTWGGGFFKTFDEAFFKFSSNCSYMFFQD-CRSSYEEF-----NIKISHGANEQFSEILFKIDTDVAQFANGKVSFNNRILTLPFSNNKILIQTHGSGVKLAFRQLEISAYLNDDAFT--VVAGDEFRQQLCGLCGNFDGSSTVTTSYSRLKMLLSFSAYEVLFFVKCSQLHLNLKTLPAIQQYLI------QPKSSGKCTAVVTSSCKGSCGL-NQEGYAAMCAIEKCQC--GSATTCGCAALSAYSRECSLQYKPLPNWRSSQMCPPQDCPGNLEYQECGSTCPPTCANM-----DPSKCQEPCVAGCVCPSGTVLSAINEETCIAPEECRCN--YNGITYDASDKITIDCNSCTCEAGSWTCTTRLCPKAC 356 HSP 3 Score: 81.6481 bits (200), Expect = 1.851e-15 Identity = 117/474 (24.68%), Postives = 189/474 (39.87%), Query Frame = 2
Query: 260 NRCSDPICVGGCS----------NGGKCVAPNQCLCLDN---YSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGI-IVSSVGSFIKIKGIDDLEILFDRDRNIYVYA----PVTAEKKIC---GLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW-IDRDPSDL-NVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLG--RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYS-TFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQL-GMELECESQCL--PGCYCPRNRYW-DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEE 1588
++C +P CV GC N C+AP +C C N Y + T+ S + ++ TR+ CS+ G H TFD SY G + ++ + A + +C + + SIS + F N + +P+ S+ + I FI++ + +L + VY V + +C GLCG FNGI SDD+ +++ F NSW ++ D D+ V R+ PC N K L +++N+ + C V Y C+ + C C Q +C + TF Y+ AC KG+ + WR AT + +C N + +S + C + C L ++L C+ L GC C + D MCVP CPC NG+ + G ++ + C C G+ C E+++
Sbjct: 284 SKCQEP-CVAGCVCPSGTVLSAINEETCIAPEECRCNYNGITYDASDKITIDCNSCTCEAGSWTCTTRLCPKA------CSLESGAHFNTFDSR--SYTFHGVCSYYL-AVKQAHVALRALGQKLECSAKNPSDLGSISSLTDKKRIRSLEFKSNGKIISSQMSVSLPYISDSVKIFRKSSMFIQVDTSYGMMMLVQNSPTMQVYIGLIDSVMFRETVCTPTGLCGNFNGIASDDFMSSQGLVEGTAAAFANSWKVNPDCEDVGTVIRD----PCQL-----NTAVAKQAELRCAILRNSTAFFKDCHYLVDPYSYYSRCKQDACSCPSGQ-------DCITETFLS----YALACASKGLIFENWRKATD-SQLVECPNAEVYSYNANACNQTCRSLSSVDLACQESGLVAEGCQCAEGLFANDEGMCVPAVSCPCYV-NGD-ILKPGEKVTIADIPCYC-REGQLLCSESKK 723 HSP 4 Score: 72.7886 bits (177), Expect = 8.153e-13 Identity = 96/410 (23.41%), Postives = 150/410 (36.59%), Query Frame = 2
Query: 1565 WQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFV-----KISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEK------DIDKFVLSWKTNPTC--ESDRGIVEYKGACALSRSY--SDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWR--TNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEG-ETFDEAAKECVKISDCKCEF-HGRFYLPYEIRQDRCNNCTC--TNGNWICTSNDC 2731
W+C N +C+G GDPHY TFD + + F C YT V + S+ + N + R I V + +++ + +N V ++ P+ G +I T IL G + + G + G CG N DD M GV E +++ + P C ++ G +R Y +D A +C + + F C A + +FFD C +D C AL+ C + A QCA + WR T +C C + +Y G + +C + EGCFCP+G + F+ A+ CV +C C G+ + Q C C C + + C N C
Sbjct: 1953 WKC--NCQCQGW----GDPHYRTFDGQKYTFLEDCTYTLVEEKINKYA--------FSVLVDNYYFYKQTPASFVRGITVNYNGNSANISLPAAKGRQTVINKVIFNGQAVAFPFVGKG---FVITNHETQILVEIPAIGASVSFTGMMFTVDLPYSLFGNNTQGQCGTCNNARGDDCMRQSGVVEDASCCAVTAADWMVPDASKPHCRAKAPTGQKPCPSTTPTTRPYCTADTAT-ICDIITSNRAFAGCAEATDLSEFFDSCVYDTCAVPT----ALE-VGCASLQVAAEQCAALGFYVDWRSLTRGMCQYNC-SSGFEYQPIGPKEQLSCDYSGATIQTSILEEGCFCPKGMKMFNSQAQTCV--VECGCMGPQGKPVMFGNSWQSDCKECVCDKASASVRCAPNKC 2336 HSP 5 Score: 64.3142 bits (155), Expect = 3.325e-10 Identity = 95/413 (23.00%), Postives = 156/413 (37.77%), Query Frame = 2
Query: 3023 QCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIY----LNRVFQFVRGSNPIVSR------NTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKD---IVNFADSWKV--------HSSCPVPQKPPFMCSKRPDREPWAKAK----CEILKSD-SFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWR--TNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE---CVERKACECVDPITNLKYNTGDKVIRECEECNC--YEGAFRCTKLFSKECLHTSRPEISTKP 4162
+C+ C+ WG+ HY +FDG+ + F C+Y + E+ NK L+ + +S + I Y N +G ++++ +P G F T+ + G V + M + + N G CG + +D +SG +E V AD W V + P QKP S P P+ A C+I+ S+ +F C + ++ ++ CV D C + C +++ +C + WR T C + C+ ++ G SC G + + + EGCFCP G+ N++ CV C C+ P G+ +C+EC C + RC E PE S KP
Sbjct: 1954 KCNCQCQGWGDPHYRTFDGQKYTFLEDCTYTLVEEKI-NKYAFSVLVDNYYFYKQTPASFVRGITVNYNGNSANISLPAAKGRQTVINKVIFNGQAVAFPFVG-KGFVITNHETQILVEIPAIGASVSFTGMMFTVDLPYSLFGNNTQGQCGTCNNARGDDCMRQSGVVEDASCCAVTAAD-WMVPDASKPHCRAKAPTGQKP--CPSTTPTTRPYCTADTATICDIITSNRAFAGCAEATDLSEFFDSCVYDTCAVPTALEV--GCASLQVAAEQCAALGFYVDWRSLTRGMCQYNCSSGFEYQPIGPKEQLSCDYSGATIQTS-----ILEEGCFCPKGMKMFNSQAQTCVVE--CGCMGP-QGKPVMFGNSWQSDCKECVCDKASASVRCAPNKCPE-----NPEHSCKP 2346
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008652.1 (pep scaffold:Pmarinus_7.0:GL477625:577100:586386:1 gene:ENSPMAG00000007836.1 transcript:ENSPMAT00000008652.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 338.576 bits (867), Expect = 5.213e-101 Identity = 220/659 (33.38%), Postives = 314/659 (47.65%), Query Frame = 2
Query: 1949 RLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWK-----TNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQE-SKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSND--CEGKEFCPKSMAWFDC-GICQKTCTNLD----LVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISE------DFCGNKL--------GTFKLISENIACGXXXXXCTKSIKFIYLNR----VFQ--FVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVN-FADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC 3820
++ + G+ I+W+G R+ + + V G+CG F ++ DD MT G E ++ F WK TN E++ + C + A + C+KL ++ F CH+ N D F CT++VCT GAL EC+ F+ YA CA + L+ WR C CP + M Y QC S C STC L C EEC EGC CP+G D CV S CKC + Y P I Q C NCTC G W C S++ + CP++M W C C TC N++ C C GC C V +C++P CPCY + + +I +C N C+C W CE K+C G+C +G+ HY++FDG+ ++FQG+C YV++ D+ L F L EN+ CGSS +CTK+I Y +F+ VRG N + PT + + + T G+ ++WD + L I + KY V GLCGNF+ ++D + +++ N F DSWKVH+ C C P R W++ KC +L+S F C VV V Y RC+ DAC CD G DC C CTA+ Y C+ + ++WR++E C
Sbjct: 1 QVTLDNGMRIVWDGVRRLYVTAPPELEGTVAGLCGTFTRDQRDDFMTRAGDVEIEVWAFTARWKVAQGATNSCPEANS--LALASPCNTYAQLNSAAQETCNKLM-NDPFNLCHTRVNPDAFKKDCTYEVCT-----AGALTGAECKTFADYAYICASRGVVLANWRLLLDCTVTCPGD-MVYQQCASSCRSTCASLSNIEDCHEECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSSTNYAPGSILQMGCKNCTCEYGGWSCWSSEGCVDDSGLCPENMQWSPCKSSCPVTCGNMNNPSLSSCITETCD-AGCECSTGLVWDGSKCVQPANCPCYHGGQSFQTGQSIT---LDCNN-CVCNGQVWECEH-KRCPGVCSVYGDPHYKTFDGRAYDFQGNCQYVLARTIYSLIDYSSLNLTDMGDVDNDGFVLTVENVPCGSSGVTCTKNIGLKYWRAHNGDIFKIDLVRGKNVV----------DPTGNFIIRRAGLYLFVDTPIGVSIQWDYSTRLYIRLHPKYMGLVEGLCGNFNGDQNDDFVTPAAGPPEEMPNIFGDSWKVHAYCAPAVVINDTCLTHPHRAAWSQRKCGVLRSSLFADCQGVVAVAPYVDRCLYDACGCDSGGDCECLCTAIAAYAYECSANGVPIEWRSDELC 634 HSP 2 Score: 162.155 bits (409), Expect = 2.188e-41 Identity = 115/406 (28.33%), Postives = 180/406 (44.33%), Query Frame = 2
Query: 1274 WRSATTICKQPQCFNGQEFSECGSNCGKNCGQL---GMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLS--------ITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMT-FKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKK 2455
W S + C ++S C S+C CG + + C GC C WDG+ CV CPC +G ++Q G + L+C C+C W+C E++ C G C + GDPHY TFD R + F G+C+Y T + S+ + + +T++N C + T C + IG+ + K++ V+ TGN I + + L + + GV I W+ +R+ I+L KY V G+CGNFNG++ DD +T G E+ + F SWK + C V C + ++ + C L S F C + D+C +D C C +G C C A ++YA +C+ + + WR++ELC K
Sbjct: 255 WSSEGCVDDSGLCPENMQWSPCKSSCPVTCGNMNNPSLSSCITETCDAGCECSTGLVWDGSKCVQPANCPCY--HGGQSFQTGQSITLDCNNCVCNGQ-VWEC-EHKRCPGVCSVYGDPHYKTFDGRAYDFQGNCQYVLARTIYSLIDYSSLNLTDMGDVDNDGFVLTVENVPCGSSGVT-CTKNIGLKYWRAHN--------GDIFKIDLVRGKNVVDPTGN---FIIRRAGLYLFVDTPIGVSIQWDYSTRLYIRLHPKYMGLVEGLCGNFNGDQNDDFVTPAAGPPEEMPNIFGDSWKVHAYCAP---AVVINDTCLTHPHRAAWSQRKCGVL-RSSLFADCQGVVAVAPYVDRCLYDACGC---DSGGDCECLCTAIAAYAYECSANGVPIEWRSDELCRKS 637 HSP 3 Score: 104.375 bits (259), Expect = 7.248e-23 Identity = 75/256 (29.30%), Postives = 110/256 (42.97%), Query Frame = 2
Query: 3362 GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVH----SSCPVPQKPPFM--CSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDG--LHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRC 4102
G+++ WD L + P + + V GLCG F +D R+G++E ++ F WKV +SCP C+ A+ C L +D F CH VN + + K C + C G C Y C + L WR +C TC G V++ C + C +C + N+ D C + C EGC CPDG L + CV++ C+C TN Y G + C+ C C G + C
Sbjct: 7 GMRIVWDGVRRLYVTAPPELEGTVAGLCGTFTRDQRDDFMTRAGDVEIEVWAFTARWKVAQGATNSCPEANSLALASPCNTYAQLNSAAQETCNKLMNDPFNLCHTRVNPDAFKKDCTYEVCT--AGALTGAECKTFADYAYICASRGVVLANWRLLLDCTVTCPGDMVYQQCASSCRSTCASL-SNIED---CHEECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSSTN--YAPGSILQMGCKNCTCEYGGWSC 254 HSP 4 Score: 82.8037 bits (203), Expect = 4.645e-16 Identity = 70/273 (25.64%), Postives = 106/273 (38.83%), Query Frame = 2
Query: 791 LEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRERE---QYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRY--WDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEEC 1591
+ I++D R +YV AP E + GLCG F DD+ ++ V F W + + PCN N+ A C ++ N+ C + V D + C E+C T CK Y+Y C +G+ + +WR C + +C S+C C L +C +C GC CP +DG CV Q +C C + + Y G L++ C+ C C +G W C +E C
Sbjct: 8 MRIVWDGVRRLYVTAPPELEGTVAGLCGTFTRDQRDDFMTRAGDVEIEVWAFTARWKVAQGATNSCPEANSLALASPCNTYAQLNS----AAQETCNKLM-NDPFNLCHTRVNPDAFKKDCTYEVC----------TAGALTGAECKTFADYAYICASRGVVLANWRLLLDC--TVTCPGDMVYQQCASSCRSTCASLSNIEDCHEECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSS--TNYAPGSILQMGCKNCTC-EYGGWSCWSSEGC 260
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005510.1 (pep scaffold:Pmarinus_7.0:GL476588:620363:669144:-1 gene:ENSPMAG00000004964.1 transcript:ENSPMAT00000005510.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 296.59 bits (758), Expect = 1.795e-81 Identity = 312/1310 (23.82%), Postives = 502/1310 (38.32%), Query Frame = 2
Query: 299 NGGKCVAPNQCLCLDN-----------YSGPNCNTLLIKSSQSDVSNYGAVTRVNNT----------MYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTI-ILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRER-EQYPCNALKLKNNDQYQKALTLCQDMV-KNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGE-STYQQGYRLKLNC-ETCICGMH-----GKWQCIENEEC-------------EGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWK----TNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKC-EFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNL---DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFC--GNKLGTFKLISEN-IACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIV-SRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS---------CPVPQKP--PFMCSKRPDREPWAKAKCEILKSDS--FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVD 4015
+G KCV +QC CLDN + +C+ I + +V+ V + T S +C + H TFD + YT + C ++ L +FN + S ++ V K LV + PF G+ + GS++ ++ +L + FD + ++ + P ++CG+CG FNG SDD + + NSW + SD + + E PC+ +N+ ++ T C + + C + + + D C ++C + + C+ + Y+ C G+ V WR+ T C P C G + C C C C QC GC C G CVP ++C C+ +NG + + +C + CIC G W C ++E C G C I+GDPHYITFD+ F G C YTA + + + L + ++ TN T V + D L + V+ ++++ P +G V I Q+S + L+++ + + ++G +EI + +Y ++ G+CGNFNG+ DD++ G ++ SW+ ++ C+ G + C + ++ C + + E FK+CH+ FFD C D+C +G+L C+A +YA QCA + ++WR N CP C Y C C +TC C ++C EGC C +G F + +CV + C C +F +++ P +WF+ G C + CT L D+ C +C+ E+ +L D R P +C I D ++ +FD F G C+Y + C + L F + + N GS++ S K + F +G +V + P G ++ +L T G+ VK+D LEI P Y NK+CGLCGN++ +D +G++ DSWK V KP P + P E K CE++ S S F KCH ++ E + K C+ D C+ + + C + Y C I + WR CP C + C + CP +C DI D C EGC C G ++ CV +AC C D
Sbjct: 44 SGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCEAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAAIPCNQSSTL-PPHFNVETTNEFRGGSTAVSYVKEVHVDVYGHRFTLAKGRRVLVDGLRVTPPFFMSGVYIQLSGSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSSELGNSWRVFNSSDDDCQDDTGEIPPCD----ENDKNLYESDTFCGIITDPEGAFKECHAVLPPQIFFDNCVFDLC----------ATGGDHGSLCQALQAYADQCAQAGVIVTWRN-NTFCPFP-CEPGSHYESCAPPCPATCSNANPPAGCNQQCAEGCVCDDGFVLSGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCGAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAAIPCNQSSTLPH-------FNVETTNEFRGGSTAVSYVKEVHV--DVYGHRFTLAKGRRVLVDGLRVTPPVFMSG---VYI-QLSGSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSNELGNSWRVFNSSDDDCQDGTGEIP---PCDENDKDVYESDSFCGIIIDDEGAFKECHAVLPPQVFFDNCVFDLCATD-GDHGSL----CQALQAYADQCAQAGVIVTWRNNTFCPFPC-EPGSHYESCAPPCPATCSNASLPGGCNQQCAEGCVCDDG--FVLSGDKCVPVELCGCLDFDNQYH---------------------------------PLGESWFESGNCSRRCTCLGAGDVHCEDWECEEHEVCSVEDGIL-----------------------DCYAREPGDC---VITGDPHYI--------------------TFDKTTHHFMGECTYTAAVP-CNQSSTLPHFNVETTNEFRGGSTTVSYVKEVHVEVYGYRFTLAKGRRVLVDDKRVKTPIEGLVPGVKIGISGSYVLLETDFGVTVKFDGKHHLEINTPSTYFNKLCGLCGNYNQNGTDDYLMPNGKLATSAAQLGDSWKAEGDDDAGSTLWVGEVSCKPDEPHIPGCDPINEEAYKKMCEVVISTSGAFAKCHAEIDPEPFLKTCIYDMCQFHGMESTL--CDNFQAYAEACRAKGINILWRNETFCPLPCPPNTHYTPCASPCPATCSDI--YAEDGCQSAARCAEGCVCDQGFVLSDDLCVSLEACGCWD 1228 HSP 2 Score: 209.92 bits (533), Expect = 1.273e-54 Identity = 216/886 (24.38%), Postives = 346/886 (39.05%), Query Frame = 2
Query: 299 NGGKCVAPNQCLCLDN-----------YSGPNCNTLLIKSSQSDVSNYGAVTRVNNT----------MYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYR-EREQYPCNALKLKNNDQYQKALTLCQDMVKNNLG-RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVR-DN-----GESTYQQGYRLKLNC-ETCICGMHGK-----WQCIENEECE-------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQV---AINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKG--ACALSRSYSDYANKVCHKLYE---------SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKE--ECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYE--IRQDRC-NNCTCT-NGNWICTSNDCEGKEFC 2749
+G KCV +QC CLDN + +C+ I + +V+ V + T S +C + H TFD + YT + C ++ L + + + T + + V L K LV + P G+ + GS++ ++ +L + FD + ++ + P ++CG+CG FNG SDD + + NSW + SD + E PC+ +N+ ++ + C ++ + + C + + + D C ++C + + + C+ + Y+ C G+ V WR+ T C P C G + C C C + C QC GC C G CVP E C C+ DN GES ++ G NC C C G W+C E+E C G C I+GDPHYITFD+ F G C YTA + + S T+ + N + +E T + E +V L + V+ ++ P + G + +S + L+++ GV + ++G +EI Y K+ G+CGN+N N DD + G + SWK + D G + G +C + + + + Y+ S F KCH+ + + F C +D+C + ++ C+ F +YA C + IN+ WR CP CP N Y C S C +TC + E C+ C EGC C +G F + CV + C C Y +E D C C C+ GN C ++ C E C
Sbjct: 431 SGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCGAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAAIPCNQSSTLPHFNVETTNEFRGGSTAVSYVKEVHVDVYGHRFTL-AKGRRVLVDGLRVTPPVFMSGVYIQLSGSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSNELGNSWRVFNSSDDDCQDGTGEIPPCD----ENDKDVYESDSFCGIIIDDEGAFKECHAVLPPQVFFDNCVFDLC----------ATDGDHGSLCQALQAYADQCAQAGVIVTWRN-NTFCPFP-CEPGSHYESCAPPCPATCSNASLPGGCNQQCAEGCVCDDGFVLSGDKCVPVELCGCLDFDNQYHPLGESWFESG-----NCSRRCTCLGAGDVHCEDWECEEHEVCSVEDGILDCYAREPGDCVITGDPHYITFDKTTHHFMGECTYTAAVPCNQ------------SSTLPHFNVETTNEFRGGSTTVSYVKEVHVEVYGYRFTLAKGRRVLVDDKRVKTPIE--GLVPGVKIGISGSYVLLETDFGVTVKFDGKHHLEINTPSTYFNKLCGLCGNYNQNGTDDYLMPNGKLATSAAQLGDSWKAEG--DDDAGSTLWVGEVSCKPDEPHIPGCDPINEEAYKKMCEVVISTSGAFAKCHAEIDPEPFLKTCIYDMC-----QFHGMESTLCDNFQAYAEACRAKGINILWRNETFCPLPCP-PNTHYTPCASPCPATCSDIYAEDGCQSAARCAEGCVCDQG--FVLSDDLCVSLEACGCWDDENNYHSFEEAWLSDNCKRKCVCSVPGNASCEAHSCVEHESC 1270 HSP 3 Score: 176.022 bits (445), Expect = 3.081e-44 Identity = 180/751 (23.97%), Postives = 304/751 (40.48%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNS--EGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLK-------NNDQYQKALTLCQDMVKNNLG-RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECES--QCLPGCYCPRNRYWDGTMCVPQEKCPCVRD-NGESTYQQGYRLKLNCE-TCICGMHG-----KWQCIENEECE-------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDI-LRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTC----ESDRGIVEYKGACALS---RSYSDYANKVCHKLYE--SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEG 2575
+C + H TFD + YT + C ++ L + + + T + + V L K LV + + K P G+ + GS++ ++ + + FD ++ + P T K+CGLCG +N +DDY N + + +SW D + C + N + Y+K +C+ ++ + C +E+ + +L C ++C+ ST C + Y+ AC KGI + WR+ T C P C ++ C S C C + E C+S +C GC C + +CV E C C D N ++++ + L NC+ C+C + G C+E+E C+ C ISGDPHY TFD+R + F G YT V + D N+ + P+ I +N N + + + I + ++N V+ P N K ++ V T + L+++ G+ ++++ S ++ + Y +V G+CGN NG++ D+ T G D F SW+ N +S R + E + LS +++ NK ++ + F +C A D ++ C +D+C N C + Y C E I +WR C ++C N+ Y + S C STC L S C+ +EGC C G
Sbjct: 890 DCVITGDPHYITFDKTTHHFMGECTYTAAVPCNQSSTLPHFNVETTNEFRGGSTTVSYVKEVHVEVYGYRFTL-AKGRRVLVDDKRVKTPIEGLVPGVKIGISGSYVLLETDFGVTVKFDGKHHLEINTPSTYFNKLCGLCGNYNQNGTDDYLMPNGKLATSAAQLGDSWKAEGDDDAGSTLWVGEVSCKPDEPHIPGCDPINEEAYKK---MCEVVISTSGAFAKCHAEIDPEPFLKTCIYDMCQ----------FHGMESTLCDNFQAYAEACRAKGINILWRNET-FCPLP-CPPNTHYTPCASPCPATCSDIYAEDGCQSAARCAEGCVCDQGFVLSDDLCVSLEACGCWDDENNYHSFEEAW-LSDNCKRKCVCSVPGNASCEAHSCVEHESCQLNNGKESCQPVSYSTCSISGDPHYQTFDKRLYHFMGRETYTLVQSCGD-----NNNLLPIRILGKNKRVGHNHRVSFLDAVYIYV----YGFKIKFTDRKDFELNGVRTKPP----SNPKEGLEIVHTSHKMVLRTDFGLTVIFDRKSHADVTIPSSYAGQVCGLCGNSNGDKSDEFKTPDGSLVTSEDVFGNSWEVNDRRSLFEQSKRLVEEPRNDLNLSCSDEQWAELENKSSCGVFHDPAGPFVQCWIPAPVDPYYTNCMYDMC-----ENIGDKELFCSSLEVYTSACQQEGITPNNWRIYTGCAQQCAAHNI-YVERMSACPSTCGDLAASSNCELPDVEGCQCMPG 1604 HSP 4 Score: 172.555 bits (436), Expect = 3.386e-43 Identity = 141/511 (27.59%), Postives = 217/511 (42.47%), Query Frame = 2
Query: 1310 CFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGE-STYQQGYRLKLNC-ETCICGMHG-----KWQCIENEEC-------------EGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWK----TNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLP----YEIRQDRCNNCTCTN-GNWICTSNDCEGKEFC 2749
C G + C C C C QC GC C G CVP ++C C+ +NG + + +C + CIC G W C ++E C G C I+GDPHYITFD+ F G C YTA + + S+ P ++ TN T V + D L + V+ ++++ P+ +G V I Q+S + L+++ + + ++G +EI + +Y ++ G+CGNFNG+ DD++ G + SW+ ++ C+ D G + C + ++ C + + E FK+CH+ FFD C D+C G +G+L C+A +YA QCA + ++WR N CP C Y C C +TC + C ++C EGC C +G F + +CV C C + Y P + QD C C N GN C + CE E C
Sbjct: 1 CEPGSHYESCAPPCPATCSNANPPAGCNQQCAEGCVCDDGFVLSGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCEAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAA------IPCNQSSTLPPHFNVETTNEFRGGSTAVSYVKEVHV--DVYGHRFTLAKGRRVLVDGLRVTPPFFMSG---VYI-QLSGSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSSELGNSWRVFNSSDDDCQDDTGEIP---PCDENDKNLYESDTFCGIITDPEGAFKECHAVLPPQIFFDNCVFDLCATG-GDHGSL----CQALQAYADQCAQAGVIVTWRNNTFCPFPC-EPGSHYESCAPPCPATCSNANPPAGCNQQCAEGCVCDDG--FVLSGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCGAWVCEDDETC 488 HSP 5 Score: 144.05 bits (362), Expect = 1.633e-34 Identity = 131/511 (25.64%), Postives = 207/511 (40.51%), Query Frame = 2
Query: 2783 CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKD------GKWVCEQNKQCS-------------GICKTWGETHYESFDGKYFEFQGSCSYVISEDFC--GNKLGTFKLISEN-IACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP------VPQKPPFMCSKRPDREPWAKAKCEILKSD--SFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVD------PITNLKYNTGDKVIR--------------ECEE---CNCYEGAFRCTKLFSKECLHTSRPEIST 4156
C TC+N + Q GC C + VL +C+ +C C NN Y +C CIC + G WVCE ++ C G C G+ HY +FD F G C+Y + C + L F + + N GS++ S K + F +G +V PP + Y ++ +L T + V++D +EI++P +Y ++CG+CGNF+ +D G +SW+V +S + PP C + + + C I+ D +FK+CH V+ + ++ CV D C D D C A++ Y +C + + + WR N CP C +++C CP +C N + GC + C EGC C DG ++CV + C C+D P+ + +G+ R ECEE C+ +G C +C+ T P T
Sbjct: 401 CPATCSNANPPAGCNQQCAEGCVCDDGFVLSGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCGAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAAIP-CNQSSTLPHFNVETTNEFRGGSTAVSYVKEVHVDVYGHRFTLAKGRRVLVDGLRVTPPVFMSGVYIQLS-GSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSNELGNSWRVFNSSDDDCQDGTGEIPP--CDENDKDVYESDSFCGIIIDDEGAFKECHAVLPPQVFFDNCVFDLCATD--GDHGSLCQALQAYADQCAQAGVIVTWRNNTFCPFPCEPGSHYESCAPPCPATC----SNASLPGGCNQQCAEGCVCDDGFVLSGDKCVPVELCGCLDFDNQYHPLGESWFESGNCSRRCTCLGAGDVHCEDWECEEHEVCSVEDGILDCYAREPGDCVITGDPHYIT 901 HSP 6 Score: 137.887 bits (346), Expect = 1.166e-32 Identity = 118/462 (25.54%), Postives = 187/462 (40.48%), Query Frame = 2
Query: 2783 CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDG------KWVCEQNKQCS-------------GICKTWGETHYESFDGKYFEFQGSCSY--VISEDFCGNKLGTFKLISEN-IACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCP------VPQKPPFMCSKRPDREPWAKAKCEIL--KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECN 4078
C TC+N + Q GC C + VL +C+ +C C NN Y +C CIC + WVCE ++ C G C G+ HY +FD F G C+Y I + F + + N GS++ S K + F +G +V PP + Y ++ +L T + V++D +EI++P +Y ++CG+CGNF+ +D G +SW+V +S + PP C + + C I+ +FK+CH V+ + ++ CV D C G D C A++ Y +C + + + WR N CP C +++C CP +C N GC + C EGC C DG ++CV C C+D N Y+ + E ++C+
Sbjct: 14 CPATCSNANPPAGCNQQCAEGCVCDDGFVLSGDKCVPTDQCGCLDNNGHYHPIGESWFESQDCSQRCICVNPGNVNCEAWVCEDDETCGVEDGILGCHGSAPGDCVITGDPHYITFDKTTHHFMGECTYTAAIPCNQSSTLPPHFNVETTNEFRGGSTAVSYVKEVHVDVYGHRFTLAKGRRVLVDGLRVTPPFFMSGVYIQLS-GSYVVLETDFNLVVRFDGNHHVEIVVPGEYAGQLCGICGNFNGDGSDDNLKPDGSAAASSSELGNSWRVFNSSDDDCQDDTGEIPP--CDENDKNLYESDTFCGIITDPEGAFKECHAVLPPQIFFDNCVFDLCA--TGGDHGSLCQALQAYADQCAQAGVIVTWRNNTFCPFPCEPGSHYESCAPPCPATC----SNANPPAGCNQQCAEGCVCDDGFVLSGDKCVPTDQCGCLD--NNGHYHPIGESWFESQDCS 464 HSP 7 Score: 105.145 bits (261), Expect = 1.091e-22 Identity = 108/464 (23.28%), Postives = 186/464 (40.09%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNL--DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICK-DGKWVCEQN--------------KQCSGI----CKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNY--------PPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREP------------WA----KAKCEILK--SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNEC 3985
CP + + C C TC+++ + C GC C + VL + C+ C C+ + Y + NC+ C+C G CE + + C + C G+ HY++FD + + F G +Y + + CG N L +++ +N G + + +L+ V+ +V G + ++ PP P +L T G+ V +D+ ++ IP Y +VCGLCGN + ++ + G + F +SW+V+ ++ F SKR EP WA K+ C + + F +C V+ YY C+ D CE + GD + FC+++E Y + C + I WR C C ++ + CP +C D+ ++ C+ +EGC C G +C
Sbjct: 1169 CPPNTHYTPCASPCPATCSDIYAEDGCQSAARCAEGCVCDQGFVLSDDLCVSLEACGCWDDENNYHSFEEAWLS-DNCKRKCVCSVPGNASCEAHSCVEHESCQLNNGKESCQPVSYSTCSISGDPHYQTFDKRLYHFMGRETYTLVQS-CGDNNNLLPIRILGKNKRVG-------HNHRVSFLDAVYIYVYGFKIKFTDRKDFELNGVRTKPPSNPKEGLEIVHTSHKMVLRTDFGLTVIFDRKSHADVTIPSSYAGQVCGLCGNSNGDKSDEFKTPDGSLVTSEDVFGNSWEVND-----RRSLFEQSKRLVEEPRNDLNLSCSDEQWAELENKSSCGVFHDPAGPFVQCWIPAPVDPYYTNCMYDMCE-NIGDKEL-FCSSLEVYTSACQQEGITPNNWRIYTGCAQQCAAHNIYVERMSACPSTCGDLAA----SSNCELPDVEGCQCMPGFVMSGFDC 1612 HSP 8 Score: 80.1073 bits (196), Expect = 5.180e-15 Identity = 84/332 (25.30%), Postives = 131/332 (39.46%), Query Frame = 2
Query: 446 TYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNS-EGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRER-EQYPCNALKLKNNDQYQKAL---TLCQDMVKNNLGR--TCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYC 1420
+Y CS+ H +TFD ++ + R YTL C L + + ++ I V I + +F L +TK P N EG+ + + ++ L ++FDR + V P + ++CGLCG NG SD+++ + + + F NSW D L +R + P N L L +D+ L + C + + G C +D Y C ++CE + + FC E Y+ AC +GI + T C Q QC + E S C CG L CE + GC C
Sbjct: 1283 SYSTCSISGDPHYQTFDKRLYHFMGRETYTLVQSCGDNNNLLPIRILGKNKRVGHNHRVSFLDAVYIYVYGFKIKFTDRKDFEL-NGVRTKPPSNPKEGLEIVHTSHKMVLRTDFGLTVIFDRKSHADVTIPSSYAGQVCGLCGNSNGDKSDEFKTPDGSLVTSEDVFGNSWEVNDRRSLFEQSKRLVEEPRNDLNLSCSDEQWAELENKSSCG-VFHDPAGPFVQCWIPAPVDPYYTNCMYDMCENIGDK----------ELFCSSLEVYTSACQQEGITPNNWRIYTGCAQ-QCAAHNIYVERMSACPSTCGDLAASSNCELPDVEGCQC 1601
BLAST of von Willebrand factor vs. Ensembl Nematostella
Match: EDO44199 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWN6]) HSP 1 Score: 361.303 bits (926), Expect = 1.380e-101 Identity = 319/1191 (26.78%), Postives = 511/1191 (42.91%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIIL----FPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAP------VTAEKKICGLCGYFNGIMSDDY--RDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQY---QKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTL-KGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMEL-ECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVL-----IDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDR----GIVEYKGACALSRSYSDYANKVCHKLYE-SEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWR-TNELCPKK--CPNENMKYNQCGS-------RCTSTCRLLQ---ESKCKEECIEGCFC-PEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGI-------CKTWGETHYESFDGKYFEFQG-SCSYVISEDFCGNKLGTFKLISENIAC----------GXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI----LFTMEGI-----------QVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSD-SFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC 3820
H TFD +S+ Y +D +F V N +C C+ S+ + V + + L ++ F V +P+ + G ++ ++ D++ I FD + + P +++ ++ GLCG ++G ++D+ D + N+ F S +D DP N + P D + Q A +C +++++ C+S+V + C ++C C NST + C + YS AC I + WRS + +C C +G E SEC S C ++C + + C S+C+ GC+CP + D CV +C C Y G K C +C+C G W C + C G C + G+ H+ TFD R + G C+Y S++ SN T +I I+ I + Q+ ++L ++ + + V+ S P N K ++ + + + + G+ ILW G + I + ++ + G+CG FN N+ DD T G +E D F W+ + + + C+ S YA C+ + + + FK CH + +C D C C C C ++YA+ CA + I + WR T +C K CP + QC RC STC L ++ C+EGC+C EGE + K CV + C C + Y EIR+DRCNNCTC G + CT+ DCE + + + DCGI TC NL + + + GC+CP+ ++H NG C++ +C C NNK Y D P +C C D W +Q +GI C +G+ HY +FDG + F+ C Y E K++ +N+ C + + ++++ + + + F R +YPP P + + ++T I LF + I ++++D+ + +M Y+ V GLCGNFD + ND R +G I I FADSW+V S+C + K C PDR+PWA CE ++++ +F CHD VN Y + C + C G DC FC+A+ YV CN Y I + WR C
Sbjct: 485 HFTTFDHQEYSFYGTCSYKALIDNSNVKQIFQVVLNNDKNC---DINAPCTKSVDVNVDGKKVHLGQEVASRHGFVKVDGVTENLPYTKSWPNIRQAGKYVIVRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTRLKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLD-DPLCTNPPKP----PSGCYSEVAGDVFSAMQNAEKMCS-VIQSDAFSACRSKVDFRDFKAQCIADVCAC------NSTSRS--DCMCNVLSTYSKACAWNHNITLSWRSPS-LCPV-TCPSGMEHSECSSACPRDCSNMNSDPNACNSKCVDGCFCPEGKIQDRGKCVDPGQCSCYHSG--IPYAHGAIRKSECSSCLCN-GGMWNCTDTP-CSGTCTMVGNSHFTTFDSRSYSMHGQCDYV----LSEHCHFSNDTTKQFTIHIK------------------ISSDGSRQLIVSLAGATPVTLGGVEGS-PTVLNSNGKATSHILSVEHIGVQNVLVHAYAGLTILWTGYNAY-ITVGPDFKSQTCGLCGTFNQNDADDFHTRDGDNEADAYAFAQEWQVVSSVAASNCHRSNWNQLDQPCSTYSSNEGYAKSGCNAIIDLNGPFKACHRYVPPQDMYQKCVKDGCMC--------KDCLCSVAAAYAKACADKGIVIDGWRDTISVCKPKFVCPAGTV-LKQCNRDPLTRKLRCPSTCDDLANVTDTCPSRRCVEGCYCEKEGELMNNEHK-CVDKTQCPCYYGDTMYKYGEIRKDRCNNCTCKGGVFDCTNVDCEAMCL-TRGLVYSDCGI---TCENLHPINGGERPCVSGCYCPDGLIMHDNGTCVQSMQCQCKHNNKYY---DAGAISPTDCSRKCR-GDRYW-----EQLAGIQAQPEYECSAFGKGHYRTFDGMLYNFESEGCGYTFMEH------EDVKVVVKNMKCVNSFELQMCKEVTITLVKENVEVVMMQKSFTLKRNGIDTAYNPGSYPP--PCKPFLNTYMNTLEIFQVGLFMIVRIVDRVNPNHIKFEIRFDQGTRVYVMANTDYKGTVQGLCGNFDGKDSNDKRTSTGSIATSIPEFADSWRVGSAC-LDTKTVSPCVLYPDRKPWADKGCEEIRANPNFTICHDAVNPAPYIEACKYETCMSHMGGDCGAFCSAIAAYVRACNRYGISILWRREGFC 1596 HSP 2 Score: 161.384 bits (407), Expect = 1.525e-39 Identity = 118/400 (29.50%), Postives = 185/400 (46.25%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSE---IKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYR------EKVTGMCGNFNGNEMDDSMTFKGV--DEKDIDKFVLSWKTNPTCESDRGIVE--YKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCAL-ENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQE--SKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG 2737
E G C+ISG H+ TFD + + F G C Y A L+ NS + + + N + N + P T V + D +V + +S +KV+ V +LPY + I Q ++ +++ V I ++G + IK+ Y+ ++ G+CGN++GN +D + G + +I+ F +S +P C + Y S A K+C + +S+ F C S + F QC DVC C + + C C S+Y++ CA NI LSWR+ LCP CP+ M++++C S C C + + C +C++GCFCPEG+ D +CV C C G Y IR+ C++C C G W CT C G
Sbjct: 473 EVPGLCYISGRGHFTTFDHQEYSFYGTCSYKA--------LIDNSNVKQIFQVVLNND--KNCDINAPCTKSVDVNVDGKKVHLGQEVASRHGFVKVDGVTENLPYTKSWPN---IRQAGKYVI-VRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTRLKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLDDPLCTNPPKPPSGCYSEVAGDVFSAMQNAEKMC-SVIQSDAFSACRSKVDFRDFKAQCIADVCAC---NSTSRSDCMCNVLSTYSKACAWNHNITLSWRSPSLCPVTCPS-GMEHSECSSACPRDCSNMNSDPNACNSKCVDGCFCPEGKIQDRG--KCVDPGQCSCYHSGIPYAHGAIRKSECSSCLCNGGMWNCTDTPCSG 851 HSP 3 Score: 144.436 bits (363), Expect = 2.115e-34 Identity = 133/491 (27.09%), Postives = 200/491 (40.73%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNL--DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI-----------LFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQ-------KPPFMCSKRPDREPWAKAKCEIL--KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENC---PHTCTGKRVWKNCGN-------ICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNE--CVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKL 4111
CP M +C C + C+N+ D +C + GCFCPE + G+C+ P +C CY + Y+ +R + C + C+C G W C + CSG C G +H+ +FD + + G C YV+SE C T K + +I I ++ + G+ P+ G PT +H + + G+ + W + I + ++++ CGLCG F+ +D R G+ E D FA W+V SS + CS E +AK+ C + + FK CH V + Y++CV D C C C C+ Y C + I + WR + C V K C CP +C D+ NVTD ++ C+EGC+C NNE CV++ C C T KY G+ C C C G F CT +
Sbjct: 759 CPSGMEHSECSSACPRDCSNMNSDPNACNSKC-VDGCFCPEGKIQDRGKCVDPGQCSCYHSGIPYAHG--AIR-KSECSS-CLCNGGMWNC-TDTPCSGTCTMVGNSHFTTFDSRSYSMHGQCDYVLSE-HCHFSNDTTKQFTIHI-----------KISSDGSRQLIVSLAGATPVTLGGVE---GSPTVLNSNGKATSHILSVEHIGVQNVLVHAYAGLTILW-TGYNAYITVGPDFKSQTCGLCGTFNQNDADDFHTRDGDNEADAYAFAQEWQVVSSVAASNCHRSNWNQLDQPCSTYSSNEGYAKSGCNAIIDLNGPFKACHRYVPPQDMYQKCVKDGCMCKD-----CLCSVAAAYAKACADKGIVIDGWRDTISVCKPKFVCPAGTVLKQCNRDPLTRKLRCPSTCDDLA-NVTDTCPSRR-CVEGCYCEKEGELMNNEHKCVDKTQCPCYYGDTMYKY--GEIRKDRCNNCTCKGGVFDCTNV 1218 HSP 4 Score: 133.65 bits (335), Expect = 4.247e-31 Identity = 101/380 (26.58%), Postives = 161/380 (42.37%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSI------KFIYLNRVFQFVRGSNPI--VSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQN------KVCGLCGNFDLRIDNDMRARSGEI--EKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPW-----AKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCN-EYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
G+C G H+ +FD + + F G+CSY D N F+++ N ++ CTKS+ K ++L + G + V+ N Y P + I+ T + + + +D + L I +P+ Y+ ++ GLCGN+D ND G + +I +F S C P KPP C + + A+ C +++SD+F C V+ + +C+AD C C+ C C + Y C + I L WR+ CP TC C + CP C ++ +D C C++GCFCP+G +CV+ C C + + Y G EC C C G + CT
Sbjct: 476 GLCYISGRGHFTTFDHQEYSFYGTCSYKALIDN-SNVKQIFQVVLNNDKNCDINAPCTKSVDVNVDGKKVHLGQEVASRHGFVKVDGVTENLPYTKSWPN----IRQAGKYVIVRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTRLKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLDDPLCTNPPKPPSGCYSEVAGDVFSAMQNAEKMCSVIQSDAFSACRSKVDFRDFKAQCIADVCACNSTSRSDCMCNVLSTYSKACAWNHNITLSWRSPSLCPVTCPSGMEHSECSSACPRDCSNMN---SDPNACNSKCVDGCFCPEGKIQDRGKCVDPGQCSCYH--SGIPYAHGAIRKSECSSCLCNGGMWNCT 845 HSP 5 Score: 104.76 bits (260), Expect = 2.376e-22 Identity = 99/392 (25.26%), Postives = 162/392 (41.33%), Query Frame = 2
Query: 1343 SNCGKNCGQLGMELECESQCLPGCYCPRNRYW-DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIEN--EECEGRCFISGDPHYITFDRRHFFFDGH-CEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVST-DILRL-----KSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRN-GALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQ 2485
S+CG C L E C+ GCYCP D CV +C C +N Y G +C C W+ + + E C G HY TFD + F+ C YT + V++ N S +Q C++ + T + V++ + + N + ++ ++ P+ +T + I QV I+R+ + EI ++ G+R+ + + Y+ V G+CGNF+G + +D T G I +F SW+ C + + C L +A+K C ++ + F CH A N + + C ++ C M + GA C A ++Y R C I++ WR C KK NM N+
Sbjct: 1232 SDCGITCENLHPINGGERPCVSGCYCPDGLIMHDNGTCVQSMQCQCKHNN--KYYDAGAISPTDCSR-KCRGDRYWEQLAGIQAQPEYECSAFGKGHYRTFDGMLYNFESEGCGYTFMEHEDVKVVVKNMKCVN-SFELQM--CKEVTITLVKENVEVVMMQKSFTLKRNGIDTAYNPGSYPPPCKPFLNTYMNTLEIFQVGLFMIVRIVDRVNPNHIKFEIRFDQGTRVYVMANTDYKGTVQGLCGNFDGKDSNDKRTSTGSIATSIPEFADSWRVGSACLDTKTV----SPCVLYPDRKPWADKGCEEIRANPNFTICHDAVNPAPYIEACKYETCMSHMGGDCGAF----CSAIAAYVRACNRYGISILWRREGFCGKKI---NMPMNK 1606 HSP 6 Score: 67.3958 bits (163), Expect = 6.792e-11 Identity = 119/481 (24.74%), Postives = 182/481 (37.84%), Query Frame = 2
Query: 281 CVGGC-------SNGGKCVAPNQCLCLDNYSG-PNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITV-GTQTII--LFPKNEFALVKNNKTKIPFNSEGIIVSSVGSF-------IKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW-IDRDPSDLNVYRE---REQYPCNALKLKNNDQYQKALTLCQDMVK-NNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQP-QCFNGQEFSECGSN-------CGKNCGQLGMELE-CES-QCLPGCYCPRNRYW--DGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISG 1615
CV GC + GKCV P QC C +SG P + + KS S G + +T S C+++ H TFD +S + Y L C F T +I +KI+ G++ +I L L + NS G S + S + + L IL+ N Y+ + + CGLCG FN +DD+ + + + F W + + N +R + PC+ +N+ Y K + C ++ N + C V C + C C ++C C + Y+ AC KGI +D WR ++CK C G +C + C C L + C S +C+ GCYC + + CV + +CPC G++ Y+ G K C C C G + C N +CE C G
Sbjct: 790 CVDGCFCPEGKIQDRGKCVDPGQCSCY--HSGIPYAHGAIRKSECSSCLCNGGMWNCTDTPCSG--TCTMVGNSHFTTFDSRSYSMHGQCDYVLSEHCH----------------FSNDTTKQFTIHIKISSDGSRQLIVSLAGATPVTLGGVEGSPTVLNSNGKATSHILSVEHIGVQNVLVHAYAGLTILW-TGYNAYITVGPDFKSQTCGLCGTFNQNDADDFHTRDGDNEADAYAFAQEWQVVSSVAASNCHRSNWNQLDQPCST--YSSNEGYAK--SGCNAIIDLNGPFKACHRYVPPQDMYQKCVKDGCMC----------KDC---LCSVAAAYAKACADKGIVIDGWRDTISVCKPKFVCPAGTVLKQCNRDPLTRKLRCPSTCDDLANVTDTCPSRRCVEGCYCEKEGELMNNEHKCVDKTQCPCYY--GDTMYKYGEIRKDRCNNCTC-KGGVFDCT-NVDCEAMCLTRG 1228
BLAST of von Willebrand factor vs. Ensembl Nematostella
Match: EDO41877 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S313]) HSP 1 Score: 207.608 bits (527), Expect = 3.097e-59 Identity = 125/333 (37.54%), Postives = 179/333 (53.75%), Query Frame = 2
Query: 2843 GCFCPENTVLHN-GECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFC-GNKLG--TFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRG-SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQN--KVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYK--RCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNE 3814
GC CP +VL + G CI ECPC+ N K Y +D I R +C N C C W C + K+C C +G+ HY++FDG+ + FQG C+YV+S D C G+KL +F++ +ENI CGS+ +CTKS+ + + VRG + P V++ ++ P A Y + I+ G+ V WD+ L I + Q+ KVCGLCGNFD + ND A++G E F D+W+ C +P C + RE + A C I+KS +FK CH V+ + YY+ RCV D C CD DC C C A+ Y C + +KWR ++
Sbjct: 13 GCVCPVGSVLDDFGRCILETECPCHHNGKSYKTDDVIRR---DC-NTCTCVRNTWECTK-KRCRATCTAFGDPHYKTFDGRLYSFQGGCNYVMSTDKCPGSKLTLQSFRIDAENIPCGSAGVTCTKSVTVTLHDTIIHMVRGKAVPSVTKVSSLSP--VHARYELVYAGLYVIIKAPFGLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTWRARGDCTKTMEPIHPCKQNKHRESKSHAACAIIKSGTFKPCHISVDPDPYYERCRCVYDTCGCDSVGDCECTCEAIAAYAYECLAAGVVVKWRQDK 338 HSP 2 Score: 129.028 bits (323), Expect = 3.447e-32 Identity = 100/350 (28.57%), Postives = 163/350 (46.57%), Query Frame = 2
Query: 1400 CLPGCYCPRNRYWDG-TMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS--KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNE 2440
C GC CP D C+ + +CPC NG+S Y+ ++ +C TC C + W+C + + C C GDPHY TFD R + F G C Y VM+T D S T+ I +N C T C +++ V + + + S KV+ SL H V + +K+ G+ ++W+ G+R+ I+LS ++ KV G+CGNF+G+ +D M G+ E F +W+ C +E C ++ ++ C + +S FK CH + + D ++++C TCG G + C CEA ++YA +C + + WR ++
Sbjct: 10 CKSGCVCPVGSVLDDFGRCILETECPC-HHNGKS-YKTDDVIRRDCNTCTC-VRNTWECTK-KRCRATCTAFGDPHYKTFDGRLYSFQGGCNY--VMST-DKCPGSKLTLQSFRIDAENIPCGSAGVT-CTKSVTVTLHDTIIHMVRGKAVPSVTKVS----SLSPVHARYELVYAGL----YVIIKAPFGLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTWRARGDCTK---TMEPIHPCKQNKHRESKSHAAC-AIIKSGTFKPCHISVDPDPYYERCRCVYDTCGCDSVGDCE-CTCEAIAAYAYECLAAGVVVKWRQDK 338 HSP 3 Score: 76.6406 bits (187), Expect = 1.379e-14 Identity = 66/277 (23.83%), Postives = 118/277 (42.60%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMD-CLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAE--KKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWR 1279
C+ H KTFDG ++S+ Y + D C G+ + + + C+ S+ +T+ I + V + P ++ +V + G ++ IK L +++DR +Y+ ++ K+CGLCG F+G M++DY N + + + F ++W R D E +PC +N + K+ C ++K+ + C V D Y E C C+ ++ +C T C+ Y+Y C G+ V WR
Sbjct: 74 CTAFGDPHYKTFDGRLYSFQGGCNYVMSTDKCPGSKLTLQSFRIDAENIPCGSAGVTCTKSVTVTLHDTIIHMVRGKAVPSVTKVSSLSPVHARYELVYA-GLYVIIKAPFGLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTW--RARGDCTKTME-PIHPCK----QNKHRESKSHAACA-IIKSGTFKPCHISVDPDPYY-----ERCRCVYDTCGCDSVGDCECT-CEAIAAYAYECLAAGVVVKWR 335
BLAST of von Willebrand factor vs. Ensembl Nematostella
Match: EDO41876 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S312]) HSP 1 Score: 188.348 bits (477), Expect = 3.911e-52 Identity = 130/392 (33.16%), Postives = 193/392 (49.23%), Query Frame = 2
Query: 1595 GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQV--AINLLSS-SEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDS-MTFKGVDEKDIDKFVLSW---KTNPTCESDRGIVEYKGACALSRSY-SDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRL--LQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK 2740
G C G HY TFD RH F G C YT D + S+ S+ +QN ++ C R + + IGE +V ++L+SS I+VN K+S PY+ +T V + +V + + + I ++G S I++ +S KYR G+CGN NG DD M + +F SW + N CES + + C+ + S D A ++C L SE F CH+ + F +C DVC+C + G C+C+A + Y+R CA I L WR+ LCPK+C + Y++CG C TC LQ + C E CI+GC CPEG + C+ ++ C C+ +G Y+ + CN C C G+W C+ + C GK
Sbjct: 2 GACSTWGQRHYRTFDGRHIDFIGKCSYTLAQ---DCIPGSDQ----FSLHVQNDKTCGSAGMDCERALLLYIGEKTYKVRWLVHLISSHGAIRVNGAKVSAPYK---DTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYRNNTCGLCGNNNGIPDDDLLMAVTNKPATSVAEFAESWAMPEPNEKCESPLEVT--RDVCSNASSVIRDAAKRICMVLL-SENFSACHTKVDPVPFVKRCEEDVCSC---KFGEHSGCQCQALTEYSRACASREIMLDWRSQHLCPKQCIQPKV-YSECGPACVKTCDADGLQPT-CHETCIDGCHCPEGTV--QTDNRCLPVNQCPCQHNGITYVTGTTIRVGCNTCKCRGGSWTCSKHRCPGK 373 HSP 2 Score: 166.392 bits (420), Expect = 1.292e-44 Identity = 113/374 (30.21%), Postives = 180/374 (48.13%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKL-ISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGP--TAFYWTSDVDTH----FILFTMEG--IQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDM-RARSGEIEKDIVNFADSWKV---HSSCPVPQKPPF-MCSKRPDR-EPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTK 4108
G C TWG+ HY +FDG++ +F G CSY +++D C F L + + CGS+ C +++ +Y+ VR ++S + G +A Y ++V+ + + +G + +K+D A S+++ + KY+N CGLCGN + D+D+ A + + + FA+SW + + C P + +CS AK C +L S++F CH V+ + KRC D C C G+ C C A+ +Y C EI L WR+ CP C +V+ CG C +C G T C + CI+GC CP+G +N C+ C C + Y TG + C C C G++ C+K
Sbjct: 2 GACSTWGQRHYRTFDGRHIDFIGKCSYTLAQD-CIPGSDQFSLHVQNDKTCGSAGMDCERAL-LLYIGEKTYKVRWLVHLISSHGAIRVNGAKVSAPYKDTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYRNNTCGLCGNNNGIPDDDLLMAVTNKPATSVAEFAESWAMPEPNEKCESPLEVTRDVCSNASSVIRDAAKRICMVLLSENFSACHTKVDPVPFVKRCEEDVCSCKFGEHSGCQCQALTEYSRACASREIMLDWRSQHLCPKQCIQPKVYSECGPACVKTCDADGLQPT----CHETCIDGCHCPEGTVQTDNRCLPVNQCPCQH--NGITYVTGTTIRVGCNTCKCRGGSWTCSK 367 HSP 3 Score: 134.42 bits (337), Expect = 1.263e-33 Identity = 111/400 (27.75%), Postives = 171/400 (42.75%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQT------IILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGI-DDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQK---NVKTFVNSWIDRDP-----SDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFIS 1612
CS H +TFDG + + YTL DC+ + F ++ C DC ++ + +G +T + L + V K P+ + VS V S+ I+ D+L I FD +I V CGLCG NGI DD V K +V F SW +P S L V R+ + ++ A +C ++ N C ++V ++ C++++C C E+S + C+ +YS AC + I +DWRS +C + QC + +SECG C K C G++ C C+ GC+CP C+P +CPC + NG TY G +++ C TC C G W C C G+ ++
Sbjct: 4 CSTWGQRHYRTFDGRHIDFIGKCSYTLAQDCIPGSDQFSLHVQNDKTCGSAGM--DCERALLLYIGEKTYKVRWLVHLISSHGAIRVNGAKVSAPYKDTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYRNNTCGLCGNNNGIPDDDL--LMAVTNKPATSVAEFAESWAMPEPNEKCESPLEVTRDVCSNASSVIR-------DAAKRICMVLLSENFS-ACHTKVDPVPFVKRCEEDVCSC--KFGEHSGCQ------CQALTEYSRACASREIMLDWRSQH-LCPK-QCIQPKVYSECGPACVKTCDADGLQPTCHETCIDGCHCPEGTVQTDNRCLPVNQCPC-QHNG-ITYVTGTTIRVGCNTCKC-RGGSWTC-SKHRCPGKPHVT 377
BLAST of von Willebrand factor vs. Ensembl Nematostella
Match: EDO33061 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST99]) HSP 1 Score: 134.42 bits (337), Expect = 3.586e-34 Identity = 95/299 (31.77%), Postives = 140/299 (46.82%), Query Frame = 2
Query: 1571 CIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSW-----KTNPTCESDRG--IVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELC 2446
CIE E G C GDPHY TFD R F F G+C Y L+++ S+ +++ N G +++ V IG I+L +KV+ ++ LPYQ + + ID+ ++ L SETGV+I+W+G S IE+ +S +YR G+CGNFNG DD M G + +F SW + P + I E C S + A K C + +F CH K++ C +VC C +KR CECE+ +YAR C +N++W + C
Sbjct: 47 CIECVEDMGICTSYGDPHYQTFDGRMFNFHGNCRYR---------LVADCRQGNFSVRLRSER-HHNQAVGEAKSLVVHIGS----TVIHLQRGLVVKVDRKEVQLPYQRLPSLQ--IDR-KNFMITLLSETGVKIIWDGESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSWSLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWPI--KSQFSNCHPVVKPHKYYKACISEVCKCLVKR------CECESLVTYARACRRMGVNVNWGRKKAC 320 HSP 2 Score: 107.071 bits (266), Expect = 8.248e-25 Identity = 76/276 (27.54%), Postives = 117/276 (42.39%), Query Frame = 2
Query: 3032 GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISE---NIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKV--HSSCP----VPQKP---PFMCSKRPDREPW-AKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC 3820
GIC ++G+ HY++FDG+ F F G+C Y + D C + +L SE N A G KS+ + V RG V R P + L + G+++ WD +E+ + +Y+ CGLCGNF+ +D ++G + F SW + H P P P P +R W A+ +C +KS F CH VV YYK C+++ C+C C C ++ Y C + + W + C
Sbjct: 55 GICTSYGDPHYQTFDGRMFNFHGNCRYRLVAD-CRQGNFSVRLRSERHHNQAVGE-----AKSLVVHIGSTVIHLQRGLVVKVDRKEVQLPYQRLPSLQIDRKNFMITLLSETGVKIIWDGESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSWSLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWPIKSQ-FSNCHPVVKPHKYYKACISEVCKCLVKR---CECESLVTYARACRRMGVNVNWGRKKAC 320 HSP 3 Score: 69.3218 bits (168), Expect = 3.071e-12 Identity = 64/279 (22.94%), Postives = 112/279 (40.14%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDC---SISMKITVGTQTIILFPKNEFALVKNNKTKIPFNS-EGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRER---EQYPCNALK------LKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDW 1276
H +TFDG +F++ Y L DC N S ++ N + S+ + +G+ T+I + V + ++P+ + + I + ++I++D + I V+ CGLCG FNG+ SDD+ N ++ + F SW L +R R ++ P + ++ + +A C +K+ C VK KY C E+C+CL + E C+ Y+ AC G+ V+W
Sbjct: 64 HYQTFDGRMFNFHGNCRYRLVADCRQG--------NFSVRLRSERHHNQAVGEAKSLVVHIGS-TVIHLQRGLVVKVDRKEVQLPYQRLPSLQIDRKNFMITLLSETGVKIIWDGESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSW------SLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWP-IKSQFS-NCHPVVKPHKYYKACISEVCKCLVKRCE-----------CESLVTYARACRRMGVNVNW 314
BLAST of von Willebrand factor vs. Ensembl Nematostella
Match: EDO49603 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFH4]) HSP 1 Score: 48.9062 bits (115), Expect = 2.981e-7 Identity = 25/60 (41.67%), Postives = 34/60 (56.67%), Query Frame = 2
Query: 2477 YNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYL 2653
Y+ CG+ C TC L + +C EEC GCFCP G D K CV + C C++ G+ +L
Sbjct: 6 YSSCGTMCPWTCTNLYDDDECPEECNRGCFCPRGMVVDRNGK-CVLATRCGCKYEGKMFL 64
BLAST of von Willebrand factor vs. Ensembl Medaka
Match: vwf (von Willebrand factor [Source:NCBI gene;Acc:101162365]) HSP 1 Score: 545.043 bits (1403), Expect = 2.210e-159 Identity = 372/1291 (28.81%), Postives = 580/1291 (44.93%), Query Frame = 2
Query: 455 ECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGID-DLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEY-KGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ--ESKCKEE--CIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEG------------------KEFCPKSMAWFDCGI------CQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTC---------TGKRV--WK--NCGNICPDSCIDIGKNVTDNTGCKKVCIEGC--FCPDGLHWQNNECVERKACECVDPI-------TNLKYNTGDKVIRE------CEECNCYEGAFRCTKLFSKECLHTSRPEIS 4153
CS+ +H+ TFDG ++ +P Y L DC + F+ N + + +G + + F + + +P+ S + S F K+ + + D NI + + CGLCG FN + +D+Y ++ ++ F NSW + CN+ K + L+ C + + + C V + +L +CQ E C C Q +E+CY C I +Y+ C GI + + C P+C G ++SEC +C C L ++ C+ +C+ GC CP + DG CV +C C + G + +C TC+C HG W+C NE C G C + G H+ +FD + F F G C+Y +N S+ I+N C DN + C R++ + + E+ + + L + VN + I P H G+ + I + +RL + + W+G R+ +KL+ ++ + G+CGN+NGN+ DD M+ G+ E F +WK N CES +Y CA++ +A + C + S+ F CH N F C +DVC CG +G + C C A ++YA C+ + + ++WR+ LC +C + Y CG+ C TC L E+ C+ E C EGCFCP G+ + +CV C C G+ Y P ++ D + C C NG+ C+S++ CP + +CG C +TC NLDL C C +PGC CP +TV H +CI P +CPCY NN+ Y+ TI +C N C+C++ KW C Q K C G+C++ GE HY +FDG F F G C YV+ +D C + G+F+++ EN ACG C K++ Y + G V+ P + + L + I + WD+ L + I Y+ +VCGLCGNFD ++ND + + ++E D +F +SWKV SC + P CS + + C ++ FK+C+ V+ E Y + CV AC C DC CFC V Y C+E I + WR+N+ CP +C G+ + W+ CG CP +C + C C+E C +CP G + ++ A CV+P + + G+KVI C+ C+C + C S L T P S
Sbjct: 26 RCSLFGRYHVHTFDGVLYEFPGDCSYLLAGDCSHRSFTLLGDFH-----------NGKRTGVTLFLGDAFELHLSVDGFLSQREKRLTLPYASHAVFAGSELGFYKLWSEEFGFTVTIDNAANIALTLSKHHANRTCGLCGNFNAVTADEYTAQEGLLTEDSYDFANSWAVKGADQSCRRVSNPSKSCNSTK-----GTEAILSSCSVLKTSGVFLHCAHLVPPEAFLLLCQAEACHCNQRD-----VEDCY---CPILLEYARTCHAHGILLHGWLEESKC-YPKCPIGMQYSECTKSCSTTCHSLNIQEVCKDECMDGCTCPAGKVLDGNRCVEVSECSCTHMGRH--FPPGSTVSQDCNTCVC-RHGSWECT-NEGCPGECLVVGQSHFKSFDNKFFTFTGDCQYLLAKDCVNN---------EFSVIIENVQCADNQDAVCTRSVTLSLPSLEN-MTVKLKHGGVVSVNNMDIQTPM-HYGH--LHIQRAVQSSVRLVFSDDLRLDWDGQGRVLLKLTARWTGQTCGLCGNYNGNQGDDFMSDSGLVETKPQAFGQAWKINGDCESTH---KYDPDPCAINPKRVRFAEEAC-AVIMSDVFSPCHILVNPSPFQRFCRYDVCACG---DG--EKCLCSALAAYAASCSAKGVLVNWRSALLCELECSGGQV-YEACGTVCDRTCLSLSGAEAGCEGEKVCEEGCFCPVGKYLSPSG-QCVTADLCPCLHDGQLYQPNDVYADHTSICYCENGSMRCSSSEVPSLLSDLFYDEDLGSTRERRSAHCPPPLVRTECGSGKKGLECARTCQNLDLPCVSLSC-IPGCLCPPDTVRHRRDCIAPEQCPCYHNNRPYAAGQTIT---TDC-NTCVCENRKWRCTQ-KLCDGVCRSVGEGHYITFDGLKFSFPGLCQYVLVQDMCLGEEGSFRVLVENEACGIVGRRCAKAVTVFYQGGLIVMQHGE---VTMKKPVPQSSQVEIIRSGQF---YTLLLGKHISLSWDRGTRLLVHISAAYRGRVCGLCGNFDGNVNNDFMSSNNQLEVDSSHFGNSWKVIPSCADVTQIPLPCSDNIMKLVTVEQSCRLMSGPLFKECNTQVDAEPYVEMCVEAACSCPSVGDCACFCDVVAAYAQACSERGISISWRSNDLCPMSCEELNPKISGVGEELCQWRYNTCGPACPITC-----QHPEPLQCSFTCVEACHAYCPPG------QLLDEVAMRCVNPSQCQMCVHNGQRISHGNKVILNHDNPALCKICHCENNSLSCEDCTSLSMLSTPTPTPS 1240 HSP 2 Score: 135.576 bits (340), Expect = 4.148e-31 Identity = 103/385 (26.75%), Postives = 159/385 (41.30%), Query Frame = 2
Query: 1595 GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSET-GVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQ-ESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGK 2740
GRC + G H TFD + F G C Y S S T+ + + TG +G D ++L + +++LPY + + +L SE G + + + I + LSK + + G+CGNFN D+ +G+ +D F SW +S R + +C ++ ++ C L S F C + F C + C C + ++ C C YAR C I L W C KCP M+Y++C C++TC L + CK+EC++GC CP G+ D CV++S+C C GR + P CN C C +G+W CT+ C G+
Sbjct: 25 GRCSLFGRYHVHTFDGVLYEFPGDCSYLLAGDCSHR-----------SFTLLG-DFHNGKRTGVTLFLG-------DAFELHLSVDGFLSQREKRLTLPYA----SHAVFAGSELGFYKLWSEEFGFTVTIDNAANIALTLSKHHANRTCGLCGNFNAVTADEYTAQEGLLTEDSYDFANSWAVKGADQSCRRVSNPSKSCNSTKG-TEAILSSCSVLKTSGVFLHCAHLVPPEAFLLLCQAEACHCNQRD---VEDCYCPILLEYARTCHAHGILLHGWLEESKCYPKCPI-GMQYSECTKSCSTTCHSLNIQEVCKDECMDGCTCPAGKVLD--GNRCVEVSECSCTHMGRHFPPGSTVSQDCNTCVCRHGSWECTNEGCPGE 379 HSP 3 Score: 108.227 bits (269), Expect = 7.727e-23 Identity = 113/451 (25.06%), Postives = 187/451 (41.46%), Query Frame = 2
Query: 182 TKSCSPDC----VNGVCKD---GRCSCSLG--WQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGI-IVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW-IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQL-GMELECESQ--CLPGCYCPRNRYWDGT-MCVPQEKCPCVRDN 1489
TKSCS C + VCKD C+C G GNRC + + C++ G+ P + D CNT + + + +N G EC V+ H K+FD F++ Y L DC+ F V +Q S+++ + + V N + P + + I +V S +++ DDL + +D + + + CGLCG +NG DD+ + +++ + F +W I+ D + Y + PC N + + A C ++ +++ C V + C+ ++C C E C C Y+ +C+ KG+ V+WRSA +C+ +C GQ + CG+ C + C L G E CE + C GC+CP +Y + CV + CPC+ D
Sbjct: 297 TKSCSTTCHSLNIQEVCKDECMDGCTCPAGKVLDGNRCVE-VSECSCTHMGRHFPPGSTVSQD------CNTCVCRHGSWECTNEGCPG-----------ECLVVGQSHFKSFDNKFFTFTGDCQYLLAKDCVNNE--FSVIIENVQCADNQDAVCTRSVTLSLPSLENMTVKLKHGGVVSVNNMDIQTPMHYGHLHIQRAVQSSVRLVFSDDLRLDWDGQGRVLLKLTARWTGQTCGLCGNYNGNQGDDFMSDSGLVETKPQAFGQAWKINGDCESTHKY---DPDPCAI----NPKRVRFAEEACA-VIMSDVFSPCHILVNPSPFQRFCRYDVCAC-------GDGEKC---LCSALAAYAASCSAKGVLVNWRSA-LLCEL-ECSGGQVYEACGTVCDRTCLSLSGAEAGCEGEKVCEEGCFCPVGKYLSPSGQCVTADLCPCLHDG 707 HSP 4 Score: 97.0561 bits (240), Expect = 1.880e-19 Identity = 112/436 (25.69%), Postives = 180/436 (41.28%), Query Frame = 2
Query: 311 CVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYEC-----SVIYGHHIKTFDGNVFSYPLRGGYTLFMD-CLGAAPLFYVYFNASYDCFDQKTQNDCSI-----SMKITVGTQ-TIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQD--MVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFN------GQE-----FSECGSNCGKNCGQLGMELECESQCLPGC--YCPRNRYWD--GTMCVPQEKCPCVRDNGESTYQQGYRLKLN 1531
C+AP QC C N + Q+ ++ N T C SV GH+I TFDG FS+P Y L D CLG F V C I + +TV Q +I+ E + K +P +S+ I+ S G F + + + +DR + V+ ++CGLCG F+G +++D+ N ++ + F NSW PS +V + + L +D K +T+ Q ++ L + C ++V + Y++MC + C C ++ +C + FC + Y+ AC+ +GI++ WRS + N G+E ++ CG C C Q L+C C+ C YCP + D CV +C NG+ G ++ LN
Sbjct: 812 CIAPEQCPCYHNNRP-------YAAGQTITTDCNTCVCENRKWRCTQKLCDGVCRSVGEGHYI-TFDGLKFSFPGLCQYVLVQDMCLGEEGSFRVLVE----------NEACGIVGRRCAKAVTVFYQGGLIVMQHGEVTM----KKPVPQSSQVEIIRS-GQFYTLLLGKHISLSWDRGTRLLVHISAAYRGRVCGLCGNFDGNVNNDFMSSNNQLEVDSSHFGNSW-KVIPSCADVTQ---------IPLPCSDNIMKLVTVEQSCRLMSGPLFKECNTQVDAEPYVEMCVEAACSC-------PSVGDC-ACFCDVVAAYAQACSERGISISWRSNDLCPMSCEELNPKISGVGEELCQWRYNTCGPACPITC-QHPEPLQCSFTCVEACHAYCPPGQLLDEVAMRCVNPSQCQMCVHNGQR-ISHGNKVILN 1204 HSP 5 Score: 76.6406 bits (187), Expect = 2.907e-13 Identity = 112/479 (23.38%), Postives = 179/479 (37.37%), Query Frame = 2
Query: 1586 ECEGRCFISGDPHYITFDRRHFFFDGH--CEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETG--CPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLS---KKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGI-VEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQE-------SKCKEECI----EGCFCPEGETFDEAAKECVKISDCK--CEFHGRFYLPYE---IRQDRCNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQKT--------CTNLDLVCPKGQCQL---PGCFCPENTVLHN-GECIKPFECPC 2911
EC C S H + FD DG C YT + SD+ S + + CQ+ + C + + V G + + L ++ +++LP++ G + V +L+LKS G + + S E ++ + G+CG +++ G + F+ W T + G+ + + + LS A C L+ SE F CH+ F +C C + CE S+YAR C + + WR+ LCP KCP+ +M+Y+ C + C C +Q ++ E C+ EGCFC G CV C + HG Y + ++ C C C + I CT+ C + +CG C+ C + VC C L P C + +L N G+C EC C
Sbjct: 1963 ECPCMCMGSSTNHVVRFDSLALRLDGEGLCSYTLLTVNSDSGEGSE-------VKLHTGPCQETTSYNQICMKAMEVTHGPHK----LILRDDMRAMIDREELALPWRFYGMELMRYGGV---MLQLKSNQGYMLTFTPQSN-EFTITFQGSTNSAQTAGLCGACGEEKVNILSLRNGSSTTNQPAFISDW----TAAGEDGVCLPKRQSVCLSG-----AAMGCQALH-SEVFLPCHAFIPVQIFLTKCEEQACE---------ESDACEVISAYARLCRQRGVCVDWRSPHLCPLKCPS-SMEYDACRTGCVEDCDSIQALPSDWSVARSNESCMDTPTEGCFCAGGTVLHHG--RCVSPEACGQCVDQHGHTYTHMQSWVPDENPCLICMCLDQQRINCTARPC-------NDVKAPECGACEILRERKGSMCCPEYECVCDLVNCALPEVPQCVDGQTLLLKNPGDCQPIHECVC 2397 HSP 6 Score: 62.3882 bits (150), Expect = 5.607e-9 Identity = 52/205 (25.37%), Postives = 75/205 (36.59%), Query Frame = 2
Query: 3428 KVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPV--PQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTD------NTGCKKVCIEGCFCPDGLHWQNNECVERKAC-ECVD 4015
+ GLCG N + R+G + F W V P++ S A C+ L S+ F CH + V+ + +C ACE C + Y C + + + WR+ CP C + C C + C I +D N C EGCFC G + CV +AC +CVD
Sbjct: 2104 QTAGLCGACGEEKVNILSLRNGSSTTNQPAFISDWTAAGEDGVCLPKRQSVCLSG-------AAMGCQALHSEVFLPCHAFIPVQIFLTKCEEQACEESDA------CEVISAYARLCRQRGVCVDWRSPHLCPLKCPSSMEYDACRTGCVEDCDSIQALPSDWSVARSNESCMDTPTEGCFCAGGTVLHHGRCVSPEACGQCVD 2295
BLAST of von Willebrand factor vs. Ensembl Medaka
Match: scospondin (SCO-spondin [Source:NCBI gene;Acc:101172449]) HSP 1 Score: 436.802 bits (1122), Expect = 5.486e-124 Identity = 335/1227 (27.30%), Postives = 526/1227 (42.87%), Query Frame = 2
Query: 680 KNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNC------GQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWN-GGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKC---------KEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLD---------LVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI-LFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQ----KPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKV-CIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFCARR---CNGIIECRDGEDE 4258
KN + LV P I V +G F+ ++ + + FD +Y+ GLCG +N N + ++ V SW+ +D SD + C+ ++ + ++A ++C +++N C V Y+D C C L T+ + C Y+ C + I + WR+ + +C + C GQ FS+C S+C +C G +C +C+ GC CP + C+ E CPC TYQ G ++ C TC+C G+WQC E C G+C + G TFD++ + G + L + ++T+ +T+ V+ +A++ SSS I V G +LW+ G I L KV G+CG ++ DD T +G E + F + + P + C+ YA +VC ++ + F+KCH + +F C +VC C ++R+ C C ++YA+ CA E + + WR CP +C + + +CG C +C Q + C+ GC CP G D +CV +S C C + Y P I Q+ CN C C +G + CT C+ + CP S+ + C TC++LD + P + L GC CP+ T H C+ P ECPC+ N ++Y N+TI + +C N C+C+ +W C + C G+C G+ HY +FDG+ + F G C YV++ + G F + +EN+ CGS+ +CTKS+ F + + +RG V+ P + T + F+ L + G+ + WD M + + + + +V GLCGNFD +ND G +E F +SWKV SCP + + P + P R WAK +C +L D F CH V +HYY CV D C CD G DC C CTA+ Y CN + ++WR+ E CP C +++ CG C SC + ++ C+ + C+EGCFCP G + C+ C C + G + + C+ C+C EG ++C + C +S P + E EF C C C+ +C DG DE
Sbjct: 155 KNTWGLVHKITVLFPL---WISVHWLGDFVFVESGLGVRLKFDLINTVYLTVTAEHLAATRGLCGVYN----------NNPEEASLTETV-SWLQQDCSDA----AELGHSCDIVE--DLTLRRQAESVCHQLLENPFTH-CHFRVDPAAYIDTCLYLYCS-LPTRERDGGA-------CDTLATYARECAQQHIIIMWRTPS-LCGR-VCPRGQVFSDCVSSCPPSCASPHPPGPAATMAQCREECVGGCECPPGLFLHQGTCLKAEDCPCFHR--RLTYQPGDSIQQRCNTCVC-RSGQWQCT-GERCAGQCTLVGALQVTTFDKKKYSLQG-----------GDFLFQ---LLKCTVTVNGVR----------QTLPVV----SSDLAVHRASSSFILVQTF------------------------------GALVLWHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPEGDVEMSVSAFAEKFISQPCTLPKAAPSD---PCSTYTQRISYAERVCSVIH-NPVFQKCHDVVEKEPYFRLCLSEVCACALQRS-----CHCTVLTAYAQHCAQEGVQVHWRNQTFCPVQCSGGQV-FRECGRTCGFSCFDWQHGWLCNDSSGEIGQRVCVPGCQCPTGLVQDHLG-QCVPVSLCPCVQDDKIYSPGTILQNNCNTCVCKHGAFNCTQEHCKSVQQCPGSLV-YSPRSCMLTCSSLDHQGQQQSSSVTKPSCREPLSGCVCPQGTFFHGDRCVLPDECPCHHNGQVYYTNETITK---DC-NTCVCQKRRWHCTR-LVCDGVCVATGDPHYVTFDGRRYSFLGDCQYVLATE----TSGLFSITAENVPCGSTGVTCTKSVTFSLGSTIIHLLRGKAVTVNGMPVTLPKSYSGSGLTLEKVGLFVSLSSRLGVTLMWDGGMRVYVHLAAHLRGQVGGLCGNFDEDTENDFTTHQGSVESTAEVFGNSWKVSPSCPDVENQDLQDPCL---NPHRVTWAKKRCAVLTQDLFSVCHTEVPFQHYYDWCVFDTCGCDSGGDCECLCTAIASYAEECNRRGVYVRWRSQELCPLQCEKGMIYEPCGPACSQSCPSVQQHSYSR--CEVLSCVEGCFCPAGTIHHGDGCILPLDCPCE--WEGSMFPPGTSITQRCQNCSCVEGLWQCEGV---ACPASSPPCL---------EAEFACATGRCIPSQWLCDNEDDCGDGSDE 1248 HSP 2 Score: 108.997 bits (271), Expect = 4.638e-23 Identity = 106/430 (24.65%), Postives = 173/430 (40.23%), Query Frame = 2
Query: 299 NGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIP--FNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGME--LECES-QCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQC 1573
+G +CV P++C C N N + K + V R + T C H TFDG +S+ Y L + G LF + + + T C+ S+ ++G+ TII + + V +P ++ G+ + VG F+ + + +++D +YV+ ++ GLCG F+ +D+ ++ + F NSW PS +V + Q PC N + A C ++ +L C +EV Y D C + C C + +C C Y+ C +G+ V WRS +C QC G + CG C ++C + CE C+ GC+CP G C+ CPC + S + G + C+ C C + G WQC
Sbjct: 806 HGDRCVLPDECPCHHNGQVYYTNETITKDCNTCVCQK---RRWHCTRLVCDGVCVATGDPHYVTFDGRRYSFLGDCQYVLATETSG---LFSI---TAENVPCGSTGVTCTKSVTFSLGS-TIIHLLRGKAVTVNGMPVTLPKSYSGSGLTLEKVGLFVSLSSRLGVTLMWDGGMRVYVHLAAHLRGQVGGLCGNFDEDTENDFTTHQGSVESTAEVFGNSW-KVSPSCPDVENQDLQDPC-----LNPHRVTWAKKRCA-VLTQDLFSVCHTEVPFQHYYDWCVFDTCGC-------DSGGDC-ECLCTAIASYAEECNRRGVYVRWRS-QELCPL-QCEKGMIYEPCGPACSQSCPSVQQHSYSRCEVLSCVEGCFCPAGTIHHGDGCILPLDCPCEWEG--SMFPPGTSITQRCQNCSC-VEGLWQC 1205 HSP 3 Score: 97.4413 bits (241), Expect = 1.439e-19 Identity = 92/375 (24.53%), Postives = 145/375 (38.67%), Query Frame = 2
Query: 575 VYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFAL---------------VKNNKTKIPFNSEGIIVS-SVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRD-------PSD-LNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNC-------------GQLGMELECESQCLPGCYCPRNRYWDGT-MCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENE 1585
V + + C ++ C++ VG + F K +++L V + +P S + V + SFI ++ L + ++ K+ GLCG DD+ ++ +V F +I + PSD + Y +R Y + +N +QK C D+V+ + Y +C E+C C + S C + Y+ C +G+ V WR+ T C QC GQ F ECG CG +C G++G + C+PGC CP D CVP CPCV+D + Y G L+ NC TC+C HG + C +
Sbjct: 416 VCRSGQWQCTGERCAGQCTL-----VGALQVTTFDKKKYSLQGGDFLFQLLKCTVTVNGVRQTLPVVSSDLAVHRASSSFILVQTFGALVLWHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPEGDVEMSVSAFAEKFISQPCTLPKAAPSDPCSTYTQRISYAERVCSVIHNPVFQK----CHDVVEK------------EPYFRLCLSEVCACALQR----------SCHCTVLTAYAQHCAQEGVQVHWRNQT-FCPV-QCSGGQVFRECGRTCGFSCFDWQHGWLCNDSSGEIGQRV-----CVPGCQCPTGLVQDHLGQCVPVSLCPCVQD--DKIYSPGTILQNNCNTCVC-KHGAFNCTQEH 749 HSP 4 Score: 58.9214 bits (141), Expect = 7.448e-8 Identity = 65/244 (26.64%), Postives = 100/244 (40.98%), Query Frame = 2
Query: 2441 LCP-KKCPNENMKYNQ-CGSRCTSTCR-LLQESKCKE-ECIEGCFCPEGETFDEAAKECVKISDCKCEF---HGRF-YLPYEIRQDRCNNCTCTNGNWICTSNDC------------------------------EG--------------KEFCPKSMAW---FDC-GICQKTCTNL--DLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCY-----MNNKMYSENDTIVRGPANCQNPC 2983
LCP ++C + Y Q C ++C +C L + +C + C GC CPEG+ + CV +++C+C +G + P E CN C C NG +CT + C EG ++ CP +W DC G C TC +L + C G C PGC CP V + G C+ +CPC M + ++E+ +++ A Q+ C
Sbjct: 3963 LCPGERCEDRERIYQQSCANQCPRSCTDLWEHVQCLQGACHSGCRCPEGQLLQDG--HCVPVAECRCGIPSGNGTLEFSPMEETTVDCNKCVCVNGTLLCTKHPCPVYDSWSSWSSCSVSCGLGRMTRTRTCEDTEGGPSCGETTQTESCRQQPCPDCPSWQVYSDCSGSCPYTCEDLWPNTQCLPGPC-TPGCTCPSGQVFYRGSCLDRADCPCSVLSLPMEFQSWNESSEVLQPGATVQHLC 4203 HSP 5 Score: 53.1434 bits (126), Expect = 3.511e-6 Identity = 50/160 (31.25%), Postives = 70/160 (43.75%), Query Frame = 2
Query: 2468 NMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKC--EFHGRFYLPYEIRQDR--CNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG---ICQKTCTNLDLVCPKGQCQLP-----GCFCPENTVLHNGECIKPFECPC 2911
++ CG C +C L + C+ +C GC+C EG+ A CV +C C G+ P EI + R CNNCTC +G + CT +E CP S W + C +TC + V C P G CP +HNG ++ PC
Sbjct: 2698 GTAFSSCGPSCPRSCDDL--AHCEWKCEPGCYCSEGKVLSVNATVCVARENCPCLDLNTGQRLKPGEITRARDGCNNCTCVDGRFNCT------REACPVSGGWCEWSEWTSCSRTC-GAESVSRYRSCSCPEPKAGGDHCPGEQEMHNGVGVQIQRQPC 2848 HSP 6 Score: 52.373 bits (124), Expect = 6.700e-6 Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2
Query: 2450 KKCPNENMKYNQCGSRCTSTCRLLQES-KCK--EECIEGCFCPEGETFDEAAKECVKISDCKC-EFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGI 2782
K+CP + +++ C S+C C LQ+ +C+ EC GC CP+G+ + CV++ C C + G+ + Q CNNC+C++G +C+++ C+ +W C +
Sbjct: 3404 KRCP-ASQEFDPCASQCPQRCSDLQQGLQCQGDTECQPGCRCPKGQLQQDGL--CVEVWQCDCVDSAGQIWAAGSWHQVDCNNCSCSDGKLLCSNHSCQSACVWSSWSSWAQCSV 3515
BLAST of von Willebrand factor vs. Ensembl Medaka
Match: otog (otogelin [Source:NCBI gene;Acc:101158818]) HSP 1 Score: 373.244 bits (957), Expect = 5.073e-104 Identity = 312/1227 (25.43%), Postives = 512/1227 (41.73%), Query Frame = 2
Query: 293 CSNGGKCVAPNQCLC-LDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKT------KIPFNSEGIIVSSVGSFIKIKGIDDLEILFD-RDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSY------------ACTL--KGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEI-KVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGS-RIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTN-ELCPKKCPNENMKYNQCGSRCTSTCRLLQE-SKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFY----LPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG------------ICQKTCTN--LDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKF---IYLNRVFQFVRGSNP--IVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS--SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC 3820
C NGG+CV C C L + +G C T++ N + C ++ +TFDG + +P R YTL D + S+ F +CS + + +I L + E L +N T ++P + + + + ++ + + ++ R ++Y+ CGLCG FN + DD ++ +V+ F NSW++ DP Q C + Y+ Q + +E+C L + P ++C+ + SY C + + RS P C G ++ EC S C C ++ CL GCYCP + CV CPC ++ Y G L+ C C C + G W C ++ C G C ++GD + +FD R F F C+Y VL+ + ++TIQN C + + C +++ +++ ED + + L E+ +ISLPY + I ++S+ L++++ G+++ ++ R+ ++ +++++ G+CG FNGN DD ++ G+ E F SWK + C S + C + YA+ +C L + + F CH + F QC + C CG C C + YAR C ++ + +R++ C CP M+Y+ C S C C L +C EEC EGC CP+G ++ CV S C C G Y LP ++ C+ ++ + CP + +C C++TC + L++ C + GC CP + H EC +P CPC K Y D R + C C C+DG + C + C +C +G+ HY +FDG F++ G+C + + + F + +EN+ C + C K + L + + NP ++ RN F W + + T + F E I V WD ++ + + +Q ++ GLCGNFDL+ N+MR F +SW + P + P CS P REP+AK +C +L S+ F+ CH VV+V +Y+ C+AD C C RG DC CFC++V Y RC + + WR+ C
Sbjct: 124 CLNGGQCVQ-QSCDCSLYHATGLRCQTVV------------------NLGFEREMTCRTWGQYNFETFDGLYYYFPGRCSYTLLKDS-----------HISFFVFKVHNDPECSSAPYTCQRSVSIFLPWEGEIRLHASNVTFKGRRLQLPHHLHHLQLEQISQYVLVTQQQGFTLAWEGRTGSVYIKLSPDFVGGTCGLCGNFNAEVQDDMTTSYGLLTHDVEMFGNSWMEMDP---------HQNTCPPVGSSFPSPYRPFPVSVQRV-----------------------EEVCSMLLSPP----FQSCHDFVSPLPYMASYYWKKDYYRIFFGTCFFCDASLFLPPRSV------PPCPAGLQYRECISCCPATCSLERTCIDSRLACLDGCYCPDGLIFQDGGCVEASDCPC--EHHGVFYPAGQMLQEECNNCTC-VGGVWNCTDHS-CPGECSVTGDMFFQSFDGRIFTFPASCQY---------VLVKSRLSGKFTVTIQNAPCGPDLDGACIQSVSLVVDEDP-RTEMTLSHMGEVFMAGQYRISLPYY---DDVFHIQELSSMFLQVRTAFGLQLQYSWAEFRLYLQAEERWKDDTVGLCGTFNGNIQDDFLSPSGMIESTPQLFGNSWKVSSACSSSLSVSPID-PCDSHQQAVSYASSMCDVLNQ-DLFAACHDYLSPAPFHQQCRANTCRCGTP-------CLCSTLAHYARHCRKFSVIVDFRSHIPDCAVSCP-ATMEYSSCVSSCQRRCSSLAALQRCGEECEEGCACPQGSFYNHRTHTCVHRSQCPCSLFGADYEPGDLPAQLSWSLTGFLKCSGFSFFLP------ERLCPAGQLYQNCSDGDDAFLPGRGVACERTCESHLLNIPCSSHDSCVSGCVCPHGLLKHGDECFEPAACPCLWKGKEYFPGD---RVSSPCYQ-CACQDGTFQCA-FRPCPSMCTAYGDRHYRTFDGLLFDYVGACKVYLVK---SSADAVFSVTAENVDCFDTGVICKKYLLINVGASLLSLDDHMGKPNPSSVIDRNQRL-------FIWQAGLFT-VVHFPEEDITVLWDHKTTVHVQVGPHWQGRLSGLCGNFDLKTVNEMRTPENMDSPTPQEFGNSWTATECVNSPDIRHP---CSLNPLREPYAKHQCAVLLSEVFQTCHPVVDVSWFYENCLADTCACSRGGDCECFCSSVAAYAQRCCHEGVPVDWRSPSLC 1226 HSP 2 Score: 147.902 bits (372), Expect = 6.054e-35 Identity = 117/463 (25.27%), Postives = 198/463 (42.76%), Query Frame = 2
Query: 2747 CPKSMAWFDCGIC-------QKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXX-XXCTKSIKFI------------YLNRVFQF--VRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFI-LFTMEGIQVKWDKA-MSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQK--PPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTN-ENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHW--QNNECVERKACECVDPITNLKYNTGD 4048
CP + + +C C ++TC + L C L GC+CP+ + +G C++ +CPC + Y + C N C C G W C + C G C G+ ++SFDG+ F F SC YV+ + G F + +N CG +C +S+ + ++ VF R S P + ++ + F+ + T G+Q+++ A L + E++++ GLCG F+ I +D + SG IE F +SWKV S+C P C +A + C++L D F CHD ++ ++++C A+ C C C C+ + Y C ++ + + +R++ +C +C + +C + C C + C + C EGC CP G + + + CV R C C + Y GD
Sbjct: 413 CPAGLQYRECISCCPATCSLERTCIDSRLAC------LDGCYCPDGLIFQDGGCVEASDCPCEHHGVFYPAGQMLQE---ECNN-CTCVGGVWNCT-DHSCPGECSVTGDMFFQSFDGRIFTFPASCQYVLVKSRLS---GKFTVTIQNAPCGPDLDGACIQSVSLVVDEDPRTEMTLSHMGEVFMAGQYRISLPYYDD-----------VFHIQELSSMFLQVRTAFGLQLQYSWAEFRLYLQAEERWKDDTVGLCGTFNGNIQDDFLSPSGMIESTPQLFGNSWKVSSACSSSLSVSPIDPCDSHQQAVSYASSMCDVLNQDLFAACHDYLSPAPFHQQCRANTCRCGTP----CLCSTLAHYARHCRKFSVIVDFRSHIPDCAVSCPATMEYSSCVSSCQRRCSSLAAL----QRCGEECEEGCACPQGSFYNHRTHTCVHRSQCPCS--LFGADYEPGD 840 HSP 3 Score: 117.472 bits (293), Expect = 1.136e-25 Identity = 94/378 (24.87%), Postives = 158/378 (41.80%), Query Frame = 2
Query: 3038 CKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKL-ISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWD-KAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSW-----------KVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHY---------YKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCT 4105
C+TWG+ ++E+FDG Y+ F G CSY + +D + + F + + C S+ +C +S+ I+L + ++ + + + + ++ +G + W+ + S+ I + + CGLCGNF+ + +DM G + D+ F +SW V SS P P +P + +R + C +L S F+ CHD V+ Y Y R C FC A + L R+ CP ++ C + CP +C + + D+ + C++GC+CPDGL +Q+ CVE C C + Y G + EC C C G + CT
Sbjct: 161 CRTWGQYNFETFDGLYYYFPGRCSYTLLKD---SHISFFVFKVHNDPECSSAPYTCQRSVS-IFLPWEGEIRLHASNVTFKGRRLQLPHHLHHLQLEQISQYVLVTQQQGFTLAWEGRTGSVYIKLSPDFVGGTCGLCGNFNAEVQDDMTTSYGLLTHDVEMFGNSWMEMDPHQNTCPPVGSSFPSPYRPFPVSVQR------VEEVCSMLLSPPFQSCHDFVSPLPYMASYYWKKDYYRIFFGTC---------FFCDA-----------SLFLPPRSVPPCP----AGLQYRECISCCPATC-SLERTCIDS---RLACLDGCYCPDGLIFQDGGCVEASDCPCEH--HGVFYPAGQMLQEECNNCTCVGGVWNCT 498 HSP 4 Score: 62.7734 bits (151), Expect = 4.833e-9 Identity = 57/240 (23.75%), Postives = 103/240 (42.92%), Query Frame = 2
Query: 3326 DVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEI---EKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCE----ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDG--LHWQNNE-CVERKACECVD 4015
D +++ + G++++W + + ++I + GLCG D +D+ +G + +D F DSW+V ++ + S+R D + + C +L++ +F CH V + + V D+ + C A+ YVA C+++ I ++WR CP C ++ C C D C + EGC CP+G LH Q + C+ + C C D
Sbjct: 2218 DTGNMYLIRSPAGLKLQWYHSTGM-MVIETDSKVPTMGLCGVCDGDPTDDLTLPNGSVLGAGEDPALFIDSWQVSNTTSYISQ-----SRRRDLN-CSTSDCSTCLSMLENATFTACHAFVPPSTFCEVWVRDSEYVNNQ------CVALAAYVASCHKFNICIEWRRPNYCPFICAATLRYQACLPACTSQSCPNHDFDADPEQCSGL-TEGCVCPEGTLLHRQYSALCIPPEKCACTD 2443 HSP 5 Score: 56.6102 bits (135), Expect = 3.850e-7 Identity = 83/374 (22.19%), Postives = 137/374 (36.63%), Query Frame = 2
Query: 1568 QCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRT------IGVIIGEDEDQVAINLL------SSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGV---DEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTS-TCRLLQESKCKEEC---IEGCFCPEGETFD-EAAKECVKISDCKC 2629
+C +C RC + D + ITFD Y L N T+ T+ C +SE+ + + I + V IN L ++ K F K TGN + ++S G+++ W + + + + + G+CG +G+ DD G +D F+ SW+ + T + L+ S SD + C + E+ F CH+ F C ++ + +++ +C A ++Y C NI + WR CP C ++Y C CTS +C E+C EGC CPEG + + C+ C C
Sbjct: 2108 KCCPKWDCPCRCSMFPDLNVITFDGNSVAIYKAALYIVTQ-------LPNETV-----TVMVQECPADSESLLLWNFTNLCLVSLNITHQSNHVLINRLQRRLYVNTRYAKARFKKYGFEIYDTGN-----------MYLIRSPAGLKLQWYHSTGMMV-IETDSKVPTMGLCGVCDGDPTDDLTLPNGSVLGAGEDPALFIDSWQVSNT----TSYISQSRRRDLNCSTSDCST--CLSMLENATFTACHAFVPPSTF--------CEVWVRDSEYVNN-QCVALAAYVASCHKFNICIEWRRPNYCPFIC-AATLRYQACLPACTSQSCPNHDFDADPEQCSGLTEGCVCPEGTLLHRQYSALCIPPEKCAC 2441
BLAST of von Willebrand factor vs. Ensembl Medaka
Match: FCGBP (Fc fragment of IgG binding protein [Source:NCBI gene;Acc:101155957]) HSP 1 Score: 270.396 bits (690), Expect = 3.248e-72 Identity = 297/1148 (25.87%), Postives = 470/1148 (40.94%), Query Frame = 2
Query: 740 IIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW-IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLG--RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCET-CICGMHG-----KWQCIENEECE-------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTN---CQDNSETGCPRTIGVIIGEDEDQVAINLLSSSE---IKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKT---NPTCESDRGIVEYKGACA-LSRSYSDYANK--VCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQES-KCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIK-FIY-------LNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSW------KVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDS---FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACEC 4009
+ V G F + L++ F+ +V P +CGLCG +NG M DD N F SW + P + E Q C + + QY+K LC ++++ G R C ++V + + C ++C E C+ Y+ AC G+ V WR+ + C +C + C S C +C L C + C GC C G CVP +C C+ ++ + + CE C C G K++C NE+C+ G C SGDPHY +FD + F F G C Y S + + ++ + S+ ++N + + R + V + L+ S+ + VN V ++P + + +V + Q T+ L + + G+ + ++ + + + YR+KV G+CGNFNG+ DD + KDI+ F SWK N CE+ E CA ++ D +K C L + F CHS + +FD C DVC NG C++ ++YA C + +N++ WRT CP KCP N Y C CT +C L + +C E C EGC C G +D + CVK ++C C G+ Y P E+ + DC + L+C + CPE+T + +K CY + N + Q C + G+ VC + + C WG+ HY +FDG F+FQG+C Y+IS+ CG N L F + N G+++ S + + FIY N+V Q + N + G + S + ++ T G+ V +D L I +P Y + VCGLCGNF+ ++++ SG+ ++ + SW K H +K C +A C L + + FK C+ ++ + + CV D C ++GD + C A+ Y +C + I +K WR NCP C +++C + C SC + T C C+E C C +G C+ K C C
Sbjct: 525 VSVYKSGWFAVVTTDFGLKVSFNWHSAAFVTLPSNYMGAVCGLCGNYNGNMQDDLTPKNGNTPVKPTDFGTSWRVAEIPGCI----EGCQGVCPSCDITQKVQYEKE-DLC-GIIRDTKGPFRDCHAKVDPAGFFEDCVYDVC----------MYEGRKDVLCQAVTAYTSACQGVGVNVYSWRT-SNFCAV-KCPIHSHYDICASACPASCEGLAPPPGCRAHCEEGCSCDEGYILSGDQCVPFSQCGCLYNDLYYRINEVFYPNGQCEEECKCTQDGEVTCKKFKCGPNEKCKIENGIQKCHPVGKGVCRASGDPHYTSFDGQKFDFQGTCTYV----LSKSCGVEDTHLEAFSVQVENVQWDRMKGKKKVSVTRLVAVEV------FGFTLIMKSKMFGVLVNGVLNNIPL-NLNDGEVQVYQEGTNYL-IATNFGLLVTYDLVYHVTVTVPGNYRDKVCGLCGNFNGDRKDDFQMPDQILAKDINVFGKSWKVAIPNVVCENGCQGNE----CADCDPAHKDVFSKPAYCGILTAPKGPFAVCHSKLDPQSYFDDCVFDVCA----SNGD-GKVLCDSVAAYAYNCHMAGVNVNDWRTPSFCPMKCP-ANSHYEVCAETCTYSCPGLSDMVQCPEACTEGCVCDAGFLYD--GQTCVKETECGCYDRGKTYKPGEVTYEE-------------------------------DCSLACSCNPATGLICEEYS-------CPEDTQCMIKKGVK----ECYNKDPCKDANCRV-------QETCRVEGGEAVCI--PKYTSTCWAWGDPHYHTFDGFDFDFQGTCKYIISKT-CGNLNGLVPFSITERNDNRGNAAVSFVREVDVFIYDYNITIRKNQVGQVTVNGELL---NLPFQLGDNEVSVFQS--GSSAVVETKFGLIVSYDWNWHLVIKLPSSYYSSVCGLCGNFNGDTGDELQNPSGKAVASVIEWGKSWQTPDQDKGHPCWDTCEKNCPTCDDDERELYKTQAFCGGLAAKTNSVFKICNSKLDPQAFMNNCVYDMC-LNKGDKRM-LCQALTSYNKQCRDEGIIIKDWRKEFNCPMNCQRFSHYEHCASPCQPSCPFPEQKQT----CSGACVEACVCDEGYVLSAGVCIPAKTCGC 1567 HSP 2 Score: 197.978 bits (502), Expect = 3.947e-50 Identity = 201/831 (24.19%), Postives = 323/831 (38.87%), Query Frame = 2
Query: 446 TYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQN-DCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNS-EGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYP-CNALKLKNNDQYQKALTLCQDMVKNNLG--RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQG---YRLKLNCETCICGMHG-----KWQCIENEEC-------------EGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN-------------PTCESDRGIV-EYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTC-RLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDR--CNNCTCTNGNWI-CTSNDCEGKEFCPKSMAWFDCGICQKTCT 2800
+Y C+ H TFDG F++ Y L C L V FN + + ++ ++ + ++ ++ T+ + +N + L +P S + + V GS I+ + I +D + V P +CGLCG +NG DD N + SW + + Q P C A YQ A C ++ + G R C V Y++ C ++C+ + + CK E Y AC +GI + WR+ C +C ++ C S C C + C C C C + G CVP C C NG+ YQ+G Y E CICG +G K +C E C EG+C SGDPHY +FD + F F G C Y DN ++ + P ++T Q N++ G + +++ S + VN +++LP + + +R ++ G+++L++ +E+ + Y+ K+ G+CGN+N + D+ G K++++F SW + P CE ++ E +C + ++ F+ CHS + + C DVC G + C + +Y C + + WR++ CP CP N Y C C TC +Q+ C E C EGC C G FD A +CV + +C C GR+ ++ D+ + C C + C C E C C I Q CT
Sbjct: 1625 SYATCTASGDPHYHTFDGRRFNFQGTCVYQLAGLCSQHPGL--VPFNVTVENENRGSKAVSFTKTVTFSIYGSTLTISKENPYKL-NGQLAALPLKSNDELAVFLSGSQAVIETAAGITITYDWWSTVRVTVPSNYHGAMCGLCGNYNGKALDDMTMQNGKTTPDEAKLGESW---QVASVPGCSSACQGPWCQACSDSQKKVYQ-ASQFC-GIIADETGPFRECHKFVDPAAYVEDCVYDVCQ----------FHGHHGSVCKAVEVYLSACQSQGIPIHPWRT-QAFCPM-ECPANSHYNLCASGCPTTCASINSLTTCHMHCSEACECDQGHLMSGDTCVPVRDCGCSY-NGQ-YYQKGDVFYPEDTCMEKCICGENGAVSCQKEKCRRGETCKLVNGVKGCHPEEEGKCVASGDPHYTSFDGQRFDFQGTCVYVLAKVCDDN----DNQLTPFTVT------QGNAKYGNGK--------------VSVTKSVTVTVNNEELNLPLSLDKGRLTVTREGHNTFVR--TDFGLKVLYDTVYYVEVIVPSTYQGKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSWVVDLPEHICGGCEEDCPVCEKANAVLYEKLDSCGIMKA-------------PDGPFQACHSKIDPAAYVSDCVFDVCAAGGSK-----EILCNSVQAYVLACQNAGVQIQPWRSSSFCPASCP-PNSHYEVCADTCRGTCASFIQQLTCSESCFEGCECDAGFVFDGA--QCVPLENCGCVHDGRYLTVNQVVVDKDCQSRCVCEASGLVKCQRLSCSSTEVCDVRGGVRSCHIKQAHCT 2386 HSP 3 Score: 195.667 bits (496), Expect = 2.172e-49 Identity = 203/799 (25.41%), Postives = 308/799 (38.55%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYF-----NASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFN-SEG-IIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYRER--EQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPC-----VRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEEC----------------------------EG-----------RCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN----------------PTCESD-RGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLL-QESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLP 2656
H +FDG F + Y L C G F N +D K + + + + V T+I+ K LV IP N ++G + V G+ I L + +D ++ V P K+CGLCG FNG DD++ +Q++ K++ F SW P NV E + C + D + K C S++ Y D C ++C N + C Y+Y C + G+ V DWR+ + C +C + C C +C L ++C C GC C +DG CV + +C C GE TY++ L +C + ++ C E+ +C EG C+ GDPHY TFD F F G C+Y + T N+ + + P SIT +N N + N+ R + V I + + N + ++ VN ++LP+Q N + S+ ++ K V WN + IKL Y V G+CGNFNG+ D+ G + ++ SW+T PTC+ D R + + + C + N V FK C+S + F + C +D+C N C+A +SY +QC E I + WR CP C Y C S C +C Q+ C C+E C C EG +A C+ C C + GR+Y P
Sbjct: 824 HYTSFDGQKFDFQGTCTYVLSKSC-GVEDTHLEAFSVQVENVQWDRMKGKKKVSVTRLVAVEVFGFTLIMKSKMFGVLVNGVLNNIPLNLNDGEVQVYQEGTNYLIATNFGLLVTYDLVYHVTVTVPGNYRDKVCGLCGNFNGDRKDDFQMPDQILAKDINVFGKSWKVAIP---NVVCENGCQGNECADCDPAHKDVFSKPAYCGILTAPKGPFAVCHSKLDPQSYFDDCVFDVCA------SNGDGK----VLCDSVAAYAYNCHMAGVNVNDWRTPS-FCPM-KCPANSHYEVCAETCTYSCPGLSDMVQCPEACTEGCVCDAGFLYDGQTCVKETECGCYDRGKTYKPGEVTYEEDCSLACSCNPATGLICEEYSCPEDTQCMIKKGVKECYNKDPCKDANCRVQETCRVEGGEAVCIPKYTSTCWAWGDPHYHTFDGFDFDFQGTCKYI-ISKTCGNL----NGLVPFSITERNDN-RGNAAVSFVREVDVFIYDYNITIRKNQVG--QVTVNGELLNLPFQLGDNEVSVFQSGSSAVVETKFGLIVSYDWNW--HLVIKLPSSYYSSVCGLCGNFNGDTGDELQNPSGKAVASVIEWGKSWQTPDQDKGHPCWDTCEKNCPTCDDDERELYKTQAFCG---GLAAKTNSV---------FKICNSKLDPQAFMNNCVYDMCL-----NKGDKRMLCQALTSYNKQCRDEGIIIKDWRKEFNCPMNC-QRFSHYEHCASPCQPSCPFPEQKQTCSGACVEACVCDEGYVL--SAGVCIPAKTCGCSYQGRYYTP 1576 HSP 4 Score: 186.037 bits (471), Expect = 1.467e-46 Identity = 189/785 (24.08%), Postives = 318/785 (40.51%), Query Frame = 2
Query: 458 CSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKIT-VGTQTIILFPKNEFAL--------VKNNKTKIP--FNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSW----IDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLG--RTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAV-DWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCET---CI------------CGMHGKWQCIEN-EECEG----RCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQV---AINLLSSS--EIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTN--PTC-ESDRGIVEYKGACALSRSYSDYANKVCHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEI 2665
C + H +TFDG F + Y + +C + L F+ QN+ S +++ VG T+ ++ A+ V N+ +P N+ + + G + I+ L + +D N+ V P T K CGLCG FNG DD+ + F +SW + DP R C + +++ L+ C + + G + C + + YL+ C+ ++C + C E Y+ AC G+ + DWR P+C ++ CGS C C +C+ C+ C C G+ CVP KC C D Q + NC+ C+ CG+ + + ++ +C+ C +GDPHY+TFD + + F G C Y S + L+ P + +QN + R V+ Q+ ++N+L+ + V+ V+ + +G V V+TD G+++ +N S + L Y V G+CGN+NGN DD G F SW+ P C E +G+ +C +++ +C + +++ F+ CH+ + FF+ C +DVC +++ C+A ++Y C +N+ SWRT+ C KCP + Y+ C S C ++C L C+ C EGC C EG + +CV S C C ++ +Y E+
Sbjct: 47 CWAMGDPHYRTFDGQRFDFMGTCTYVIAKNCGEDSRL---------TAFEVHAQNENRGSHRVSYVGLVTVQVYDTTITAVRSETGRVRVDNSLWSLPIILNNGKLKIFQSGRSVVIEADFGLMVRYDWTHNLVVTLPDTYSGKTCGLCGNFNGNPKDDFTTPSGTQASGAVAFGSSWKVPGLVADPQ----CRNDCVGGCGSCTESQLNKWDSDLS-CGIITRKENGPFKNCHAIIDPKAYLENCKYDLC----------MGKGLRQFLCNALETYAEACQAAGVQIGDWRGLAKC--SPRCPANSKYELCGSACPATCSDPDAPSKCQRPCVETCTCKEGFVLSGSECVPATKCGCTYDGRYIPAGQTFWADQNCKQLCKCVAGTRQVACQDKGCGVGQQCKVVDGIRKCQALSYSTCKATGDPHYVTFDNKKYDFQGTCVYQLAALCSKDTELT-----PFEVLVQNEH----------RGRNVVSYTKLVQIKVYSLNILNDQLVNLPVSLVEGKVSVYKSGWFAV----VTTDF-------GLKVSFNWHSAAFVTLPSNYMGAVCGLCGNYNGNMQDDLTPKNGNTPVKPTDFGTSWRVAEIPGCIEGCQGVCP---SCDITQKVQYEKEDLCGIIRDTKGPFRDCHAKVDPAGFFEDCVYDVCMYEGRKD-----VLCQAVTAYTSACQGVGVNVYSWRTSNFCAVKCPIHS-HYDICASACPASCEGLAPPPGCRAHCEEGCSCDEGYIL--SGDQCVPFSQCGCLYNDLYYRINEV 768 HSP 5 Score: 144.821 bits (364), Expect = 6.340e-34 Identity = 124/437 (28.38%), Postives = 184/437 (42.11%), Query Frame = 2
Query: 2843 GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCIC-KDG-----KWVCEQNKQCS-------------GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCG---NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFV-----RGSNPIVSRNTNYPP----GGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKR------PDREPWAK------AKCEILKS--DSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVD 4015
GC C E +L +C+ +C C N+ Y N+ C+ C C +DG K+ C N++C G+C+ G+ HY SFDG+ F+FQG+C+YV+S+ CG L F + EN+ S+ + VF F + +V+ N P G Y T++++ T G+ V +D + + +P Y++KVCGLCGNF+ +D + + KDI F SWKV P +C D +P K A C IL + F CH ++ + Y+ CV D C GD V C +V Y C+ + + WRT CP C ++ C C SC ++D C + C EGC C G + CV+ C C D
Sbjct: 734 GCSCDEGYILSGDQCVPFSQCGCLYNDLYYRINEVFYPN-GQCEEECKCTQDGEVTCKKFKCGPNEKCKIENGIQKCHPVGKGVCRASGDPHYTSFDGQKFDFQGTCTYVLSKS-CGVEDTHLEAFSVQVENVQWDRMKGKKKVSVTRLVAVEVFGFTLIMKSKMFGVLVNGVLNNIPLNLNDGEVQVYQEG---TNYLIATNFGLLVTYDLVYHVTVTVPGNYRDKVCGLCGNFNGDRKDDFQMPDQILAKDINVFGKSWKV-------AIPNVVCENGCQGNECADCDPAHKDVFSKPAYCGILTAPKGPFAVCHSKLDPQSYFDDCVFDVCA-SNGDGKV-LCDSVAAYAYNCHMAGVNVNDWRTPSFCPMKCPANSHYEVCAETCTYSC----PGLSDMVQCPEACTEGCVCDAGFLYDGQTCVKETECGCYD 1152 HSP 6 Score: 142.895 bits (359), Expect = 2.420e-33 Identity = 121/465 (26.02%), Postives = 199/465 (42.80%), Query Frame = 2
Query: 2843 GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQC-------------------SGICKTWGETHYESFDGKYFEFQGSCSYVISE--DFCGNKLGTFKLISENIACGXXXXXCTKSIKFIY----LNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKV----------HSSCPVPQKPPFMCSKRPDREPWAKAKCEILKS--DSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVI--RECE-ECNC-YEGAFRCTKL 4111
C C + ++ C+ +C C N + Y + D + C CIC + V Q ++C G C G+ HY SFDG+ F+FQG+C YV+++ D N+L F + N G+ S TKS+ LN +G + N + V T F G++V +D +E+++P YQ K+CGLCGN++ + ++ R G+ K++ F SW V CPV +K + ++ D C I+K+ F+ CH ++ Y CV D C G C +V+ YV C + ++ WR++ CP +C ++ C + C +C + +T C + C EGC C G + +CV + C CV + +Y T ++V+ ++C+ C C G +C +L
Sbjct: 1932 ACECDQGHLMSGDTCVPVRDCGCSYNGQYYQKGD-VFYPEDTCMEKCICGENGAVSCQKEKCRRGETCKLVNGVKGCHPEEEGKCVASGDPHYTSFDGQRFDFQGTCVYVLAKVCDDNDNQLTPFTVTQGNAKYGNGKVSVTKSVTVTVNNEELNLPLSLDKGRLTVTREGHN------------TFVRTDF------GLKVLYDTVYYVEVIVPSTYQGKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSWVVDLPEHICGGCEEDCPVCEKANAVLYEKLD-------SCGIMKAPDGPFQACHSKIDPAAYVSDCVFDVCA--AGGSKEILCNSVQAYVLACQNAGVQIQPWRSSSFCPASCPPNSHYEVCADTCRGTCASFIQQLT----CSESCFEGCECDAGFVFDGAQCVPLENCGCVH---DGRYLTVNQVVVDKDCQSRCVCEASGLVKCQRL 2361 HSP 7 Score: 142.895 bits (359), Expect = 2.461e-33 Identity = 128/466 (27.47%), Postives = 188/466 (40.34%), Query Frame = 2
Query: 2837 LPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTI--------------VRGPANCQNP-------CICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGT--FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVD--------THFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS--SCPVPQKPPF--MCSKRPDREPWAKAKCEILKSDS--FKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRE---CEECNCYE-GAFRCTK 4108
+ C C E VL G CI C C + Y+ G C+ CI +DG+ VC+ + C G+ HY +FDG+ F FQG+C Y ++ C G F + EN GS + S TK++ F + GS +S+ Y G A D + ++ T GI + +D ++ + +P Y +CGLCGN++ + +DM ++G+ D +SW+V S C + P+ CS + A C I+ ++ F++CH V+ Y + CV D C+ V C AVE Y++ C I + WRT CP C + C + CP +C I T C C E C C G + CV + C C Y GD E E+C C E GA C K
Sbjct: 1543 VEACVCDEGYVLSAGVCIPAKTCGCSYQGRYYTPGQQFWADEACSRLCVCDTTLGMVTCREASCSVNEKCIIQDGERVCQPISYAT--CTASGDPHYHTFDGRRFNFQGTCVYQLA-GLCSQHPGLVPFNVTVENENRGSKAVSFTKTVTFS--------IYGSTLTISKENPYKLNGQLAALPLKSNDELAVFLSGSQAVIETAAGITITYDWWSTVRVTVPSNYHGAMCGLCGNYNGKALDDMTMQNGKTTPDEAKLGESWQVASVPGCSSACQGPWCQACSDSQKKVYQASQFCGIIADETGPFRECHKFVDPAAYVEDCVYDVCQFHGHHGSV--CKAVEVYLSACQSQGIPIHPWRTQAFCPMECPANSHYNLCASGCPTTCASINSLTT----CHMHCSEACECDQGHLMSGDTCVPVRDCGC--SYNGQYYQKGDVFYPEDTCMEKCICGENGAVSCQK 1989 HSP 8 Score: 126.331 bits (316), Expect = 2.565e-28 Identity = 129/501 (25.75%), Postives = 211/501 (42.12%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLP---GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDG-KWVCEQNKQCS---------GI----------CKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFY--WTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS-------------SCPVPQKPPFMCSKRPDREPWAKAKCEILK--SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIREC-EECNCYE-GAFRCTKL 4111
CP + + CG C TC++ D +CQ P C C E VL EC+ +C C + + T NC+ C C G + V Q+K C GI CK G+ HY +FD K ++FQG+C Y ++ C +L F+++ +N G + S TK ++ +V+ ++ +V+ + G + + W + V T F G++V ++ + + +P Y VCGLCGN++ + +D+ ++G +F SW+V SC + QK + ++ D C I++ F+ CH V+ +++ CV D C + D + C AV Y + C + + WRT+ C C + C + CP SC + + GC+ C EGC C +G ++CV C C+ + N +C EEC C + G C K
Sbjct: 323 CPANSKYELCGSACPATCSDPDA---PSKCQRPCVETCTCKEGFVLSGSECVPATKCGCTYDGRYIPAGQTF-WADQNCKQLCKCVAGTRQVACQDKGCGVGQQCKVVDGIRKCQALSYSTCKATGDPHYVTFDNKKYDFQGTCVYQLAA-LCSKDTELTPFEVLVQNEHRGRNVVSYTKLVQI----KVYSLNILNDQLVNLPVSLVEGKVSVYKSGWFAVVTTDF------GLKVSFNWHSAAFVTLPSNYMGAVCGLCGNYNGNMQDDLTPKNGNTPVKPTDFGTSWRVAEIPGCIEGCQGVCPSCDITQKVQY---EKEDL-------CGIIRDTKGPFRDCHAKVDPAGFFEDCVYDVCMYEGRKDVL--CQAVTAYTSACQGVGVNVYSWRTSNFCAVKCPIHSHYDICASACPASC----EGLAPPPGCRAHCEEGCSCDEGYILSGDQCVPFSQCGCLYNDLYYRINEVFYPNGQCEEECKCTQDGEVTCKKF 792 HSP 9 Score: 117.472 bits (293), Expect = 1.119e-25 Identity = 133/573 (23.21%), Postives = 202/573 (35.25%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLG----AAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEG--IIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDM-VKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHG-----KWQCIENEECEGR-----CFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKT 2143
H +FDG F + Y L C P NA Y S++ +TV V N + +P + + + V+ G ++ L++L+D + V P T + K+CGLCG +N D++R KNV F SW+ P + E + C + N Y+K L C M + + C S++ Y+ C ++C ++ C + Y AC G+ + WRS ++ C C + C C C +L C C GC C +DG CVP E C CV D T Q K C+C G + C E C+ R C I I+ + FDG + L + T + + P T+ V E + + S I VN K+S P T V + S I R ++ V + ++ + + + KV G CGN+N DD T G D+ + SW
Sbjct: 2022 HYTSFDGQRFDFQGTCVYVLAKVCDDNDNQLTPFTVTQGNAKYG------NGKVSVTKSVTV--------------TVNNEELNLPLSLDKGRLTVTREGHNTFVRTDFGLKVLYDTVYYVEVIVPSTYQGKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSWVVDLPEHICGGCEED---CPVCEKANAVLYEK-LDSCGIMKAPDGPFQACHSKIDPAAYVSDCVFDVCAAGGSK----------EILCNSVQAYVLACQNAGVQIQPWRS-SSFCPA-SCPPNSHYEVCADTCRGTCASFIQQLTCSESCFEGCECDAGFVFDGAQCVPLENCGCVHDGRYLTVNQVVVDKDCQSRCVCEASGLVKCQRLSCSSTEVCDVRGGVRSCHIKQAHCTISHAGQLSSFDGMTAAVEAQGAVEVASLCDETSENWFRVVVDVRVCHKKSAFAPATVHVFFNE----ATVTVNSQHGIWVNGKKVSPPNNLTNQLSVQVLDRSVVIQR---KSAVRVTYSVSQEVTVTVDNSLASKVCGACGNYNSESEDDLKTADGKVTTDMSVVIGSWSA 2551 HSP 10 Score: 116.316 bits (290), Expect = 2.308e-25 Identity = 98/368 (26.63%), Postives = 157/368 (42.66%), Query Frame = 2
Query: 2981 CICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCG--NKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRG------------SNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQ------KPPFMCSKRPDREPWAKAKCEIL---KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCF-CTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACEC 4009
C K+G +C + G C G+ HY +FDG+ F+F G+C+YVI+++ CG ++L F++ ++N GS S + + VR S PI+ N G + + ++ G+ V++D +L + +P+ Y K CGLCGNF+ +D SG V F SWKV PQ C++ + + C I+ ++ FK CH +++ + Y + C D C G F C A+E Y C + + WR C C ++ CG+ CP +C D + C++ C+E C C +G +ECV C C
Sbjct: 29 CEMKNGSPLCVPQSK-MGTCWAMGDPHYRTFDGQRFDFMGTCTYVIAKN-CGEDSRLTAFEVHAQNENRGSHRVSYVGLVTVQVYDTTITAVRSETGRVRVDNSLWSLPIILNN------GKLKIFQSG---RSVVIEADFGLMVRYDWTHNLVVTLPDTYSGKTCGLCGNFNGNPKDDFTTPSGTQASGAVAFGSSWKVPGLVADPQCRNDCVGGCGSCTESQLNKWDSDLSCGIITRKENGPFKNCHAIIDPKAYLENCKYDLC---MGKGLRQFLCNALETYAEACQAAGVQIGDWRGLAKCSPRCPANSKYELCGSACPATCSDPDA----PSKCQRPCVETCTCKEGFVLSGSECVPATKCGC 378 HSP 11 Score: 54.299 bits (129), Expect = 1.656e-6 Identity = 53/284 (18.66%), Postives = 104/284 (36.62%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSG--ICKTWGET----------------HYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPI-VSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKV 3538
CP + + C C+ TC + + GC C V +C+ C C + + + N +V +CQ+ C+C+ V Q CS +C G SFDG + + ++ F+++ + C S+ ++ ++ N V + I V+ PP T +D ++ ++V + + + + + +KVCG CGN++ ++D++ G++ D+ SW
Sbjct: 2271 CPPNSHYEVCADTCRGTCASFIQQLTCSESCFEGCECDAGFVFDGAQCVPLENCGCVHDGRYLTVNQVVVD--KDCQSRCVCEASGLVKCQRLSCSSTEVCDVRGGVRSCHIKQAHCTISHAGQLSSFDGMTAAVEAQGAVEVASLCDETSENWFRVVVDVRVCHKKSAFAPATVH-VFFNEATVTVNSQHGIWVNGKKVSPPNNLTNQLSVQVLDRSVVIQRKSAVRVTYSVSQEVTVTVDNSLASKVCGACGNYNSESEDDLKTADGKVTTDMSVVIGSWSA 2551
BLAST of von Willebrand factor vs. Ensembl Medaka
Match: zanl (zonadhesin [Source:NCBI gene;Acc:101162078]) HSP 1 Score: 265.003 bits (676), Expect = 1.934e-70 Identity = 282/1229 (22.95%), Postives = 484/1229 (39.38%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAA-PLFYVYFNASYDCFDQKTQNDCSISMKITVGT---QTIILFPKNEFALVKNNKTKIPFN-SEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGY-------RLKLNCE----TCICG----MH------GKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKC-EFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWV------CEQNKQC------------SGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRV-FQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEIL--KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACEC 4009
H TFD +++ Y + C + P F V+ + ++ N +IS V I K V +P N + + V G + + + +D + + + + + K+CGLCG +NG DD++ + + + F +SW + DP + ++ + + Y+ + D KN+ C +V + Y C ++CE +E C+ E Y C +G+ + WR+ T K C + + C C C G C C GC C CV + C C NG+ Q G +LK C+ TC+ MH G+ C + + C +SGDPHY TFD++ + F G C YT T ++ +P +++ N ++ +G + + ++ + + L+ S VN +++ P+ + ++ +S + L++ G+ + W+G +I + Y +++ G+CG+++GN +D G +++ F SW+T + C C K+ + +C + + FF C +D+C + NG L H C+ +Y C + WR + C CP N Y+ C S C TC ++ C++ C+EGC C G F + +CV + DC C + G ++ P CP + + +CG C TC C G C + GCFC V C+ +C C + Y E +V G C C C + C+ +++C + C G+ HY +FD ++F G+CSYV+S L F++ ++N +S + +Y++ V ++G V+ P P H+ + G+ V++D ++I + KYQ+K+CGLCG+++ +D + G + D F SW P C P + + C IL K+ F CH VN +Y++ C+ D CE + + C A+E YV C + + + NE C ++ C + C D+C GK + C C EGC C G +CV+R++C C
Sbjct: 1422 HYTTFDKRKYNFMGNCSYVMSGICNATSLPFFEVHAD------NENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPVNPTPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSW-NTDPCN-----KQPNATIPGCEPGEQELYEGYCGILLD--KNSPFAVCHGKVNPNNYFQDCLFDLCE----------LEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFCLK---CPSNSHYEPCADPCQDTCS--GKPSSCGGACSEGCVCDPGYVLSAGKCVKSDSCGCKHTNGQYYEQPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSIQ---DCIVSGDPHYNTFDKKFYSFMGTCTYTLARTCRND--------SPW-FSVEGKN-EERGASGVSYLKKLYVTVND--ITVTLMKSKRTLVNGKRVAFPHNPS---PLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISV-PSYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVIKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMC----QFNGEL-HVLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCP-ANSSYSLCVSSCPETCLGVIGSPGCEDVCVEGCECDPG--FILSDDKCVPLKDCGCVDTSGTYHPP------------------------------CPPNAEYIECGPACIPTCQEPSTNC-SGSC-ISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLG-DGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPSCYPKTSTCIASGDPHYTTFDKHRYDFMGNCSYVMSGICNTTSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGC--EPGEQELYEGYCGILLDKNGPFAVCHGKVNPNNYFQDCLFDLCELEGAKPIL--CEAIEAYVNECQDRGVDIGPWRNETFCLKCPPNSHYEPCADPCQDTC--SGK----PSSCGGACSEGCVCDPGYVLSAGKCVKRESCGC 2548 HSP 2 Score: 177.948 bits (450), Expect = 5.620e-44 Identity = 176/690 (25.51%), Postives = 285/690 (41.30%), Query Frame = 2
Query: 710 KTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECE---SQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKV-CHKLYESE-EFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKC-KEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYE--IRQDRCNNCTCTNGNWICTSNDCEGKEFC 2749
+T + ++ G V + GS+ + L++ FD ++ + P K+CG+CG +N +DD ++ K+V NSW SD R N ++ ++ C+ ++ ++ R C S V D +L C ++CE Q +T C E Y+ AC G+ + WR++T P +S+C + C C L + C + C+ GC C CV +KC CV +GE Y RC +SGDPHY TFD + G YT V+T N+ ++ PL + +N N + V + V + L + VN +++ P + L +++ ++L ++ G+ + ++G SR I L + Y V G+CGN++G ++ M G +D+++F SW+ + E+D C+ S DY + C L + E F CH AA + + C D+C +G+ + C ++ +YA C + L SWR C CP N Y C S C S+C L S+C C+EGC C G E A CV +C C F R+Y E + +D +C T+ +C C+ C
Sbjct: 3291 RTPLTIDTIGAKVITSGSYSLLDTNFGLQVKFDGVHHLEITVPGEYFNKLCGMCGNYNHDSTDDNLKPDKQPAKDVIELGNSWKSEGDSDPCQPDNRPDVHPNCTA----EEEEQFKAQCEQVILSSSFRPCHSVVSPDAFLGNCIYDMCEYNGMQ----------ATLCDNVEAYAQACQSAGVTITWRNSTFCLPCPP---NSHYSDCTAPCPPTCSDL-FPIFCHLPPTACVEGCECNAGYVLSDGKCVSLDKCGCVGSDGE--YHD---------------------------FDRCTVSGDPHYRTFDGFTHHYQG--PYTYVLTQDHNL---QGSLKPLMVRGKNLRRGGNKRVSFLDEMYVNV----YGVDVRFLQKKTVLVNGERVTPPLSPV---RGLTITMNSKQMQLTTDFGLTVRFDGKSRGVI-LPRTYSNSVRGLCGNYDGITRNEYMKPDGTVVRDLNEFGDSWRVSRDTETDPDSGFETSDCSQSE-LDDYTSVTKCGALSDPEGPFSACH-AALPPQIYQDCVFDLCA----EDGS-EELRCASYEAYATICQEAGVKLGSWRQQLGC-LPCP-ANSTYTACMSPCPSSCADLAAPSECDATACVEGCQCAAGFVMSEGA--CVPYPECGCTFLDRYYPLNENFVTEDCSQSCVSTSIGAVCQPKTCQAGYVC 3909 HSP 3 Score: 162.925 bits (411), Expect = 1.840e-39 Identity = 179/809 (22.13%), Postives = 317/809 (39.18%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDC-LGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGT---QTIILFPKNEFALVKNNKTKIPFN-SEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNG------ESTYQQGYRLKLNCE----TCICG----MH------GKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRC-----NNCTCTNGNWI-CTSNDCEG-KEFCPKSMAWFDCGICQKTCT 2800
H TFD + + + Y + C + P F V+ + ++ N +IS V I K V +P N + + V G + + + +D + + + + + K+CGLCG +NG DD++ + + + F +SW + DP + ++ + + Y+ + D KN C +V + Y C ++CE +E C+ E Y C +G+ + WR+ T K P + C C C G C C GC C CV +E C C NG E Y + +LK C+ TC+ MH G+ C + + C +SGDPHY TFD++++ F G C YT T ++ +P +++ N ++ +G + + ++ + + L+ S VN +++ P+ + ++ +S + L++ G+ + W+G +I + Y +++ G+CG+++GN +D G +++ F SW+T + C C K+ + +C + + FF C +D+C + NG L H C+ +Y C + WR + C CP N Y+ C S C TC ++ C++ C+EGC C G F + +CV + DC C Y P+++ + +C C +G I C + C E C ++C C+
Sbjct: 2240 HYTTFDKHRYDFMGNCSYVMSGICNTTSLPFFEVHAD------NENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSW-NTDPCN-----KQPNATIPGCEPGEQELYEGYCGILLD--KNGPFAVCHGKVNPNNYFQDCLFDLCE----------LEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFCLKCPP---NSHYEPCADPCQDTCS--GKPSSCGGACSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSSQ---DCIVSGDPHYNTFDKKYYSFMGTCTYTLARTCRND--------SPW-FSVEGKN-EERGASGVSYLKKLYVTVND--ITVTLMKSKRTLVNGKRVAFPHNP---SPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISV-PSYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGPFECIGVVDPEPFFQSCVYDMC----QFNGEL-HVLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCP-ANSSYSLCVSSCPETCLGVVGSPGCEDVCVEGCECDPG--FILSDDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSGGQIQCHNTSCNPVTEVCQLQDGLYECSPLNGICS 2990 HSP 4 Score: 159.458 bits (402), Expect = 2.563e-38 Identity = 170/754 (22.55%), Postives = 296/754 (39.26%), Query Frame = 2
Query: 479 HIKTFDGNVFSYPLRGGYTLFMDCLGAA-PLFYVYFNASYDCFDQKTQNDCSISMKITVGT---QTIILFPKNEFALVKNNKTKIPFN-SEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVD-WRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNG------ESTYQQGYRLKLNCE----TCICG----MH------GKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINL-SWRTNELCPKKCPNENMKYNQCGSRCTSTCR-LLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLP 2656
H TFD + + + Y + C + P F V+ + ++ N +IS V I K V +P N + + V G + + + +D + + + + + K+CGLCG +NG DD++ + + + F +SW + DP + ++ + + Y+ + D KN C +V + Y C ++CE +E C+ E Y C +G+ + WR+ T K P + C C C G C C GC C CV +E C C NG E Y + +LK C+ TC+ MH GK C + + C +SGDPHY TFD+ ++ F G C YT T ++ +P +++ N ++ +G + + + + + L+ S VN +++ P+ + ++ +S + L++ G+ + W+G +I + Y +++ G+CG+++GN +D G +++ F SW+T + C C K+ + +C + + FF C +D+C + NG L H C+ +Y C + + WR + C CP N Y+ C S C TC ++ C++ C+EGC C G F + +CV + DC C Y P
Sbjct: 442 HYTTFDKHRYDFMGNCSYLMSGICNATSLPFFEVHAD------NENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSW-NTDPCN-----KQPNATIPGCEPGEQELYEGYCGILLD--KNGPFAVCHGKVNPNNYFQDCLFDLCE----------LEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFCLKCPP---NSHYEPCADPCQDTCS--GKPSSCGGACSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKMQDGKLGCYPSFQ---DCIVSGDPHYNTFDKNYYTFMGTCTYTLARTCRND--------SPW-FSVEGKN-EERGVSGVSYLKKLYV--TVNNITVTLMKSRTTLVNGKRVAFPHNP---SPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISV-PSYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMC----QFNGEL-HVLCDQLQAYTDACQSAGVTVHHWRAPDFC-LDCP-ANSSYSLCVSSCPETCLGVVGSPGCEDVCVEGCECDPG--FILSDDKCVPLKDCGCVDTSGTYHP 1137 HSP 5 Score: 157.147 bits (396), Expect = 1.002e-37 Identity = 141/515 (27.38%), Postives = 213/515 (41.36%), Query Frame = 2
Query: 1301 QPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNC-ETCICGMHGKWQCIEN-----EECE------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL-SITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVE---------YKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEF-HGRFYLPYE--IRQDRCNNCTCTNGNWICTSNDCEGKEFC 2749
P C E+ ECG C C + C C+ GC+C + G CVP EKC C+ ++G L C + C C + +C +N EEC C SGDPHY TFD+ + F G+C Y VM+ + N+T P + N N ++ + + V + V I++L ++VN ++LP V S + GV + ++G ++IK+ KY++K+ G+CG++NGN DD G D ++F SW T+P + + Y+G C + L ++ F CH N + +F C D+C + GA CEA +Y +C +++ WR C KCP N Y C C TC + S C C EGC C G +A +CVK C C+ +G++Y P E +D C C + C ++DC C
Sbjct: 2117 HPPCPPNAEYIECGPACIPTCQE--PSTNCSGSCISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPSCYPKTSTCIASGDPHYTTFDKHRYDFMGNCSY--VMSG-----ICNTTSLPFFEVHADNENRYNSPTISYLKAVHVYV----HMVKISILKGGVVQVNGTNVNLPLNPAPEVSVF---QSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGI-------------LLDKNGPFAVCHGKVNPNNYFQDCLFDLC----ELEGA-KPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCP-PNSHYEPCADPCQDTCS-GKPSSCGGACSEGCVCDPGYVL--SAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHEC 2591 HSP 6 Score: 155.221 bits (391), Expect = 4.480e-37 Identity = 160/701 (22.82%), Postives = 266/701 (37.95%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMK--ITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSV-GSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTL---CQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQ----QGYRLKLNCE-TCICGMHGKWQCIENEECE-------------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANK--VCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELC 2446
Y + +C V H TFD +S+ YTL C +P F V +++ + S K +TV T+ L K++ LV + P N ++ S+ G ++ ++ L + +D + + + P + ++CGLCG ++G +D+ + NV F NSW + E E P L + + + +T C + + C V + + C ++C+ C + Y+ AC G V A C C +S C S+C + C + CE C+ GC C CV + C CV +G TY L CE +C C G+ QC N C G C +SGDPHY TFD+R + G C YT + S+S + ++ QN + N++ R + + + + + ++VN + P K+ S + L++ G+ + ++G ++ L Y + GMC NFN N DD++ + ++ SW R G + A K C + + FK CH+ + +F+ C +D+C G + C+A SYA CA +N+ WR + C
Sbjct: 2601 YPSSQDCIVSGDPHYNTFDKKYYSFMGTCTYTLARTCRNDSPWFSVEGKN-----EERGASGVSYLKKLYVTVNDITVTLM-KSKRTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEE-------DEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGPFECIGVVDPEPFFQSCVYDMCQ----------FNGELHVLCDQLQAYTDACQSAGATVHHWRAPDFCLD--CPANSSYSLCVSSCPETCLGVVGSPGCEDVCVEGCECDPGFILSDDKCVSLKDCGCVDTSG--TYHPFQVDDEWLLEGCEQSCECLSGGQIQC-HNTSCNPVTEVCQLQDGLYECSPLNGICSVSGDPHYTTFDKRTHHYMGACSYTLT-----KLCNSSSDLPHFTVDTQNEHRGSNTKVSYVRAVVINV----YGMTVVFGKGRSVQVNGTVVVPPVSSISGVKIYF---SGKFVVLEASFGLRVRFDGNHHADVTLPSSYSGLLCGMC-NFNDNPTDDNLKPDNTPAANSNELGDSWAV----PDPRPCTNGGGQEECDEKVEEEAQKPTSCGMITDPNIFKPCHAVVPPEPYFENCVYDMCATGGQTVAL-----CQALESYADICAAAGVNIPWRNSTFC 3250 HSP 7 Score: 154.451 bits (389), Expect = 6.998e-37 Identity = 140/508 (27.56%), Postives = 213/508 (41.93%), Query Frame = 2
Query: 1325 EFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNC-ETCICGMHGKWQCIEN-----EECE------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL-SITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVE---------YKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEF-HGRFY--LPYEIRQDRCN-NCTCTNGNWICTSNDCEGKEFC 2749
E+ ECG C C + C C+ GC+C + G CVP EKC C+ ++G L C + C C + +C +N EEC C SGDPHY TFD+R + F G+C Y VM+ + N+T P + N N ++ + + V + V I++L ++VN ++LP T V S + GV + ++G ++IK+ KY++K+ G+CG++NGN DD G D ++F SW T+P + + Y+G C + L ++ F CH N + +F C D+C + GA CEA +Y +C +++ WR C KCP+ N Y C C TC + S C C EGC C G +A +CVK C C+ +G++Y E + C C C + C ++DC C
Sbjct: 1307 EYIECGPACIPTCQE--PSTNCSGSCISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPGCYPKTSTCIASGDPHYTTFDKRKYNFMGNCSY--VMSG-----ICNATSLPFFEVHADNENRYNSPTISYLKAVHVYV----HMVKISILKGGVVQVNGTNVNLPVNPTPEVSVF---QSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGI-------------LLDKNSPFAVCHGKVNPNNYFQDCLFDLC----ELEGA-KPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPS-NSHYEPCADPCQDTCS-GKPSSCGGACSEGCVCDPGYVL--SAGKCVKSDSCGCKHTNGQYYEQPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHEC 1774 HSP 8 Score: 154.066 bits (388), Expect = 8.857e-37 Identity = 146/528 (27.65%), Postives = 220/528 (41.67%), Query Frame = 2
Query: 1262 IAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNC-ETCICGMHGKWQCIEN-----EECE------------GRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPL-SITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVE---------YKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLS-WRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEF-HGRFYLPYE--IRQDRCNNCTCTNGNWICTSNDCEGKEFC 2749
IA+D TT CK C E+ ECG C C + C C+ GC+C + G CVP EKC C+ ++G L C + C C + +C +N EEC C SGDPHY TFD+ + F G+C Y +M+ + N+T P + N N ++ + + V + V I++L ++VN ++LP V S + GV + ++G ++IK+ KY++K+ G+CG++NGN DD G D ++F SW T+P + + Y+G C + L ++ F CH N + +F C D+C + GA CEA +Y +C +++ WR C KCP N Y C C TC + S C C EGC C G +A +CVK C C+ +G++Y P E +D C C + C ++DC C
Sbjct: 309 IAIDAVCITT-CKP--CPPNAEYIECGPACIPTCQE--PSTNCSGSCISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCVGNYTLECTDNSCDPSEECREVNGVPGCYPKTSTCIASGDPHYTTFDKHRYDFMGNCSY--LMSG-----ICNATSLPFFEVHADNENRYNSPTISYLKAVHVYV----HMVKISILKGGVVQVNGTNVNLPLNPAPEVSVF---QSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGI-------------LLDKNGPFAVCHGKVNPNNYFQDCLFDLC----ELEGA-KPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCP-PNSHYEPCADPCQDTCS-GKPSSCGGACSEGCVCDPGYVL--SAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHEC 793 HSP 9 Score: 141.739 bits (356), Expect = 6.073e-33 Identity = 120/448 (26.79%), Postives = 186/448 (41.52%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSEND-------------------TIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS---SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFK-KCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVD 4015
CP + + C CQ TC+ C G C GC C VL G+C+K C C N Y E T V + C +DGK C + Q C G+ HY +FD Y+ F G+C+Y ++ C N F + +N G S S K + N ++ +V+ P P+ S + IL T G++V+WD +I +P Y +++CGLCG++D ND G ++ +F +SW+ C P C + E KC ++ S +C VV+ E +++ CV D C+ + + C ++ Y C + + WR + C C + C + CP++C+ V + GC+ VC+EGC C G +++CV K C CVD
Sbjct: 697 CPPNSHYEPCADPCQDTCSGKPSSC-GGACS-EGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKMQDGKLGCYPSFQ---DCIVSGDPHYNTFDKNYYTFMGTCTYTLART-CRNDSPWFSVEGKNEERGVSGVSYLKKLYVTVNNITVTLMKSRTTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMCQFN--GELHVLCDQLQAYTDACQSAGVTVHHWRAPDFC-LDCPANSSYSLCVSSCPETCL----GVVGSPGCEDVCVEGCECDPGFILSDDKCVPLKDCGCVD 1130 HSP 10 Score: 140.584 bits (353), Expect = 1.085e-32 Identity = 127/477 (26.62%), Postives = 199/477 (41.72%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSEND-------------------TIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS---SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFK-KCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPI-TNLKYNTGDKVIRE-CEE-CNCYEGA 4093
CP + + C CQ TC+ C G C GC C VL G+C+K C C N Y E T V + C +DG+ C + Q C G+ HY +FD KY+ F G+C+Y ++ C N F + +N G+S S K + + ++ +V+ P P+ S + IL T G++V+WD +I +P Y +++CGLCG++D ND G ++ +F +SW+ C P C + E KC ++ S +C VV+ E +++ CV D C+ + + C ++ Y C + WR + C C + C + CP++C+ V + GC+ VC+EGC C G +++CV K C CVD T + D+ + E CE+ C C G
Sbjct: 2495 CPPNSHYEPCADPCQDTCSGKPSSC-GGACS-EGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSSQ---DCIVSGDPHYNTFDKKYYSFMGTCTYTLART-CRNDSPWFSVEGKNEERGASGVSYLKKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGPFECIGVVDPEPFFQSCVYDMCQFN--GELHVLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCPANSSYSLCVSSCPETCL----GVVGSPGCEDVCVEGCECDPGFILSDDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSGG 2957 HSP 11 Score: 139.043 bits (349), Expect = 3.929e-32 Identity = 118/449 (26.28%), Postives = 188/449 (41.87%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCIC--------------------KDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHS---SCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFK-KCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLK-WRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVD 4015
CP + + C CQ TC+ C G C GC C VL G+C+K C C N Y E +C+ C C +DG+ C + Q C G+ HY +FD K++ F G+C+Y ++ C N F + +N G+S S K + + ++ +V+ P P+ S + IL T G++V+WD +I +P Y +++CGLCG++D ND G ++ +F +SW+ C P C + E KC ++ S +C VV+ E +++ CV D C+ + + C ++ Y C + WR + C C + C + CP++C+ V + GC+ VC+EGC C G +++CV K C CVD
Sbjct: 1677 CPSNSHYEPCADPCQDTCSGKPSSC-GGACS-EGCVCDPGYVLSAGKCVKSDSCGCKHTNGQYYEQPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSIQ---DCIVSGDPHYNTFDKKFYSFMGTCTYTLART-CRNDSPWFSVEGKNEERGASGVSYLKKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVIKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMCQFN--GELHVLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCPANSSYSLCVSSCPETCL----GVIGSPGCEDVCVEGCECDPGFILSDDKCVPLKDCGCVD 2111 HSP 12 Score: 126.716 bits (317), Expect = 1.950e-28 Identity = 113/443 (25.51%), Postives = 184/443 (41.53%), Query Frame = 2
Query: 2747 CPKSMAWFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWV------CEQNKQC------------SGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRV-FQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEIL--KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACEC 4009
CP + + +CG C TC C G C + GCFC V C+ +C C + Y E +V G C C C + C+ +++C + C G+ HY +FD ++F G+CSY++S L F++ ++N +S + +Y++ V ++G V+ P P H+ + G+ V++D ++I + KYQ+K+CGLCG+++ +D + G + D F SW P C P + + C IL K+ F CH VN +Y++ C+ D CE + + C A+E YV C + + + NE C ++ C + C D+C GK + C C EGC C G +CV+R++C C
Sbjct: 322 CPPNAEYIECGPACIPTCQEPSTNC-SGSC-ISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLG-DGCSKLCRCVGNYTLECTDNSCDPSEECREVNGVPGCYPKTSTCIASGDPHYTTFDKHRYDFMGNCSYLMSGICNATSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGC--EPGEQELYEGYCGILLDKNGPFAVCHGKVNPNNYFQDCLFDLCELEGAKPIL--CEAIEAYVNECQDRGVDIGPWRNETFCLKCPPNSHYEPCADPCQDTC--SGK----PSSCGGACSEGCVCDPGYVLSAGKCVKRESCGC 750 HSP 13 Score: 123.25 bits (308), Expect = 2.470e-27 Identity = 112/437 (25.63%), Postives = 180/437 (41.19%), Query Frame = 2
Query: 2765 WFDCG-ICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWV------CEQNKQC------------SGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRV-FQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEIL--KSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACEC 4009
+ +CG C TC C G C + GCFC V C+ +C C + Y E +V G C C C + C+ +++C + C G+ HY +FD + + F G+CSYV+S L F++ ++N +S + +Y++ V ++G V+ P PT H+ + G+ V++D ++I + KYQ+K+CGLCG+++ +D + G + D F SW P C P + + C IL K+ F CH VN +Y++ C+ D CE + + C A+E YV C + + + NE C ++ C + C D+C GK + C C EGC C G +CV+ +C C
Sbjct: 1308 YIECGPACIPTCQEPSTNC-SGSC-ISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLG-DGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPGCYPKTSTCIASGDPHYTTFDKRKYNFMGNCSYVMSGICNATSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPVNPTPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKV-IKYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGC--EPGEQELYEGYCGILLDKNSPFAVCHGKVNPNNYFQDCLFDLCELEGAKPIL--CEAIEAYVNECQDRGVDIGPWRNETFCLKCPSNSHYEPCADPCQDTC--SGK----PSSCGGACSEGCVCDPGYVLSAGKCVKSDSCGC 1730 HSP 14 Score: 113.235 bits (282), Expect = 2.275e-24 Identity = 113/444 (25.45%), Postives = 172/444 (38.74%), Query Frame = 2
Query: 2747 CPKSMAWFDC-GICQKTCTNLDLVCPKGQCQLP------GCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLI---SENIACGXXXXXCTKSIKFI---YLNRV---FQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFM---CSKRPDREPWAKAKCEILK--SDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGL-KWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECV 4012
CP + + DC C TC++L + C LP GC C VL +G+C+ +C C ++ Y + D C G+ HY +FDG +QG +YV+++D N G+ K + +N+ G K + F+ Y+N +F++ +V+ PP P + L T G+ V++D S +++P Y N V GLCGN+D N+ G + +D+ F DSW+V F CS+ + + KC L F CH + + Y+ CV D C D ++ C + E Y C E + L WR C C + C + CP SC D+ + C+EGC C G CV C C
Sbjct: 3474 CPPNSHYSDCTAPCPPTCSDLFPI----FCHLPPTACVEGCECNAGYVLSDGKCVSLDKCGCVGSDGEYHDFDR------------------------------CTVSGDPHYRTFDGFTHHYQGPYTYVLTQDH--NLQGSLKPLMVRGKNLRRGG-----NKRVSFLDEMYVNVYGVDVRFLQKKTVLVNGERVTPPLSPVRGLTITMNSKQMQLTTDFGLTVRFD-GKSRGVILPRTYSNSVRGLCGNYDGITRNEYMKPDGTVVRDLNEFGDSWRVSRDTETDPDSGFETSDCSQSELDDYTSVTKCGALSDPEGPFSACHAALPPQ-IYQDCVFDLCAEDGSEELR--CASYEAYATICQEAGVKLGSWRQQLGC-LPCPANSTYTACMSPCPSSCADLAAPSECDA---TACVEGCQCAAGFVMSEGACVPYPECGCT 3868 HSP 15 Score: 107.071 bits (266), Expect = 1.559e-22 Identity = 81/296 (27.36%), Postives = 124/296 (41.89%), Query Frame = 2
Query: 3152 FKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREP--------WAKAKCE-ILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECV 4012
F+++++N G +S +S+K LN R P T ++ +L T G+QVK+D LEI +P +Y NK+CG+CGN++ +D + KD++ +SWK P RPD P KA+CE ++ S SF+ CH VV+ + + C+ D CE + C VE Y C + + WR + C C + +C CP +C D+ C+EGC C G + +CV C CV
Sbjct: 3260 FRVVAKNEERGQPEASYVRSVKVFLLN-------------GRRVRTPLTIDTIGAKVITSGSYSLLDTNFGLQVKFDGVHHLEITVPGEYFNKLCGMCGNYNHDSTDDNLKPDKQPAKDVIELGNSWKSEG-----DSDPCQPDNRPDVHPNCTAEEEEQFKAQCEQVILSSSFRPCHSVVSPDAFLGNCIYDMCEYNGMQ--ATLCDNVEAYAQACQSAGVTITWRNSTFC-LPCPPNSHYSDCTAPCPPTCSDLFPIFCHLP--PTACVEGCECNAGYVLSDGKCVSLDKCGCV 3532 HSP 16 Score: 95.1301 bits (235), Expect = 7.132e-19 Identity = 72/275 (26.18%), Postives = 120/275 (43.64%), Query Frame = 2
Query: 1865 VNFVKISLPYQHTGNTKVLIDQVSTDILR------LKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKT---NPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKC---KEECIEGCFCPEGETFDEAAKECVKISDCKC-----EFH 2638
V VK+ L T + ID + ++ L + G+++ ++G +EI + +Y K+ GMCGN+N + DD++ KD+ + SWK+ + C+ D + A + C ++ S F+ CHS + D F C +D+C + NG + C+ +YA+ C + ++WR + C CP N Y+ C + C TC L C C+EGC C G + +CV + C C E+H
Sbjct: 3277 VRSVKVFLLNGRRVRTPLTIDTIGAKVITSGSYSLLDTNFGLQVKFDGVHHLEITVPGEYFNKLCGMCGNYNHDSTDDNLKPDKQPAKDVIELGNSWKSEGDSDPCQPDNRPDVHPNCTAEEE---EQFKAQCEQVILSSSFRPCHSVVSPDAFLGNCIYDMC----EYNG-MQATLCDNVEAYAQACQSAGVTITWRNSTFC-LPCP-PNSHYSDCTAPCPPTCSDLFPIFCHLPPTACVEGCECNAGYVLSDG--KCVSLDKCGCVGSDGEYH 3539 HSP 17 Score: 87.8113 bits (216), Expect = 1.251e-16 Identity = 104/450 (23.11%), Postives = 175/450 (38.89%), Query Frame = 2
Query: 2627 CEFHGRFYL---PYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTC--TNLDLVCPKGQCQ---LPGCFCPENTVLHNGECIKPFECPCYMNNKMYS----ENDTIVRGPANCQNPCICKDGKWVCEQNKQCS-------------------GICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNK--LGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTME--------GIQVKWDKAMSLEIMIPEKYQNKVCGLCGNF-------DLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVP----QKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENC 3820
C+F+G ++ + D C + T +W +FC A +C +C T L +V G C+ + GC C +L + +C+ +C C + Y +++ ++ G C+ C C G + N C+ GIC G+ HY +FD + + G+CSY +++ C + L F + ++N G + K Y+ V V G + + + G S + I F+ + G++V++D ++ +P Y +CG+C NF +L+ DN A S E+ DSW V P Q+ + ++P + I + FK CH VV E Y++ CV D C G V C A+E Y C + + WR + C
Sbjct: 2832 CQFNGELHVLCDQLQAYTDACQSAGATVHHW-------RAPDFCLDCPANSSYSLCVSSCPETCLGVVGSPG-CEDVCVEGCECDPGFILSDDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEG---CEQSCECLSGGQIQCHNTSCNPVTEVCQLQDGLYECSPLNGICSVSGDPHYTTFDKRTHHYMGACSYTLTK-LCNSSSDLPHFTVDTQNEHRG-------SNTKVSYVRAVVINVYGMTVVFGKGRSVQVNGTVVVPPVSSISGVKIYFSGKFVVLEASFGLRVRFDGNHHADVTLPSSYSGLLCGMC-NFNDNPTDDNLKPDNTPAANSNEL-------GDSWAVPDPRPCTNGGGQEECDEKVEEEAQKPTSCGM--ITDPNIFKPCHAVVPPEPYFENCVYDMCA--TGGQTVALCQALESYADICAAAGVNIPWRNSTFC 3250 HSP 18 Score: 80.1073 bits (196), Expect = 2.819e-14 Identity = 81/357 (22.69%), Postives = 138/357 (38.66%), Query Frame = 2
Query: 440 YSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMK--ITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSV-GSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTL---CQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNG 1492
Y ++ +C V H TFD N +++ YTL C +P F V +++ + S K +TV T+ L K+ LV + P N ++ S+ G ++ ++ L + +D + + + P + ++CGLCG ++G +D+ + NV F NSW + E E P L + + + +T C + + C V + + C ++C+ C + Y+ AC G+ V A C C +S C S+C + C + CE C+ GC C CVP + C CV +G
Sbjct: 803 YPSFQDCIVSGDPHYNTFDKNYYTFMGTCTYTLARTCRNDSPWFSVEGKN-----EERGVSGVSYLKKLYVTVNNITVTLM-KSRTTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEE-------DEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMCQ----------FNGELHVLCDQLQAYTDACQSAGVTVHHWRAPDFCLD--CPANSSYSLCVSSCPETCLGVVGSPGCEDVCVEGCECDPGFILSDDKCVPLKDCGCVDTSG 1133
BLAST of von Willebrand factor vs. Planmine SMEST
Match: SMESG000025547.1 (SMESG000025547.1) HSP 1 Score: 1011.52 bits (2614), Expect = 0.000e+0 Identity = 594/1622 (36.62%), Postives = 868/1622 (53.51%), Query Frame = 2
Query: 185 KSCSPDCVNGVCKDGRCSCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLDNYSGPNCNTLLIKSSQSDVSNYGAVTRVNNTMYSTYYECSVIYGHHIKTFDGNVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNG--IMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGES--TYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGL--HWQNNECVERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECLHTSRPE---ISTKPPHCVNEYEFLCPNNFCARRCNGIIECRDGEDEDLC-SXXXXXXXXXXXXNYCITDQNEIMKSGETKIVDQCIKYICDDGNIVTKTKFCNITCDEHSEILKQHVDSDKCCECVSIRTTTLMVSATTTRLVTCEMYTLTVEKNFNILYKLKSDSIGNSIENVIYDSDK-----------SWTVVSNNAVLIVEFLPKQPXXXXXXXXXXXXXXXXXXXXXDIVEKDDTWKDADSYTLAKSSTKDGNLFSYTFTSATKDGIPGRFVRFSFILSNQITEVIVIRKLMIKIC 4987
K C C+ G CK+G C C G++G RC C GC G CV PN C C +N+ G NC T + + S++ ++ V NT Y Y CS++ G ++ TFDG PLRG L +DCL P + ++FN+SY+C + N C S+ I ++++ L ++ I ++G F +IKGI +LEIL+D RN++++AP+T +K +CGLCGYF+G +S ++++ N +NV F+ W + DP D N Y + C L N D A +CQD+ +L + + + + Y +C+ E+C CL I C + C + SY CTL G ++WRS +C +C +E+SECGS+C +NC L + C+S C GC+CP++R+W+GT CVP+ +CPC+R NG S Y QG K +CE C C G + C + C G C ISGDPHY TFD+RH + +G C YT V T + N L I +QNT+C +++ C +++ + G+ ++ I L+++ I VN + LP Q ++ I +V+ D + ++S G+ ILW+ ++IEI L + Y+++V G+CGN+NGN DD+ G + ++ +SW T C+S + Y G CA S Y +A + C +L E F CH + + + F QC HD+C CG D+C CE+ +SY R C + L WR++ LC KCPN M+Y +C + C+ +C L ++ C ECI+GC C +D A +CV IS C+C +Y R+++CN+C CTNG W CT NDC FCP W +CG C +TC N D C + C+ GCFCP+ +L N C+ +CPC ++K Y I GP NC + C C +GKW CEQ ++C G CK+WG+ HY++FDGKYF+F CSYV+ + CG +G FKL++ENI CG+ +SCTKSI F Y N+V Q +RG P V N NYP D+ + I++T I + WDK +S+EI +P +YQN+VCGLCGNFD ++ND ++++G E +I+ F DSWK +CP + P MC +R+ WA C +K+ F C VV+ E YY++C++D C CDRG DC C CTA+ + + C +Y I WR+NE CP C ++ +K C N CP C N T C K C+EGC CP +N+CV+ C C P ++ KYN G+ C C C E F+C RP+ I++ +C+ C N CAR CNGI EC DG DE C ST + TT T +CI + IM+ GE ++ C+ C D IV + C+ TC + + +LK + DKCCEC I +TT VS TTT CE KL SDS I NV ++ SW V ++NA + P+ E+ ++ I + V+ E + + + YT+ K G LF + +KDG FV+F+ + +Q E + IR ++ IC
Sbjct: 117 KPCIQGCIRGKCKNGDCLCDSGYEGERCDRAFCQYGCGTG-ICVEPNVCQCPENFRGRNCETFFREPNTSNL----KISDVINTKYKDYQLCSIMGGSYVTTFDGISIENPLRGSNILVIDCLSRKPSYRIFFNSSYNCPTKDFVN-CDTSLMIETASESVTFL-----GLSNSSTNSNRNYISSIQHKNIGDFNQIKGIGELEILYDGKRNVFIFAPLTMKKSVCGLCGYFDGNSSLSSEFKNINGESIENVTEFMKYWKNPDPIDPNTYYTTGRDLCTGL---NKDLVTTANNMCQDVFGGDLSQC--TILPVIPYQSICRSELCHCLNA---GQNISVCANIACSMGSFKSYQCTLNGKLINWRSQ--LCAPRECPANKEYSECGSSCSQNCEDLITDENCKSFCTAGCFCPKDRFWNGTYCVPKPQCPCIRKNGNSMVVYHQGMSFKRDCEICECSKGGVFNCAKIRGCSGTCVISGDPHYTTFDKRHIYVEGSCFYTMVATKPS--ITGNQV---LKIWVQNTHCMKSNDAYCTKSLSLQFGDTDNSTFIQLVTTKNILVNKIPNRLPLQINDPLRLTIQEVAHDTVLIESYNGIRILWHQKTQIEITLPEFYQDQVQGLCGNYNGNSEDDATKSSGELTTNDNELAVSWAT-AECKSTLSL-SYLGVCAKSYEYEKFAKEFCQRLL-YEPFNSCHMSVDPNPFIQQCEHDICLCGGNEK---DNCACESMASYGRACMKNGLTLKWRSSSLCDPKCPN-GMEYMECKNLCSVSCFSLTQTNCSTECIDGCQCKSNMVYDTAVNQCVNISQCQCSHENAYYPSGSTRKEKCNDCNCTNGVWSCTKNDCSNVTFCPPGKEWKECGHCDQTCENQDGFCLEKGCK-SGCFCPDGYLLSNKTCVPKHKCPCLWSDKSYLNQSVIYTGPTNCLSKCTCNEGKWGCEQEQKCVGSCKSWGDAHYKTFDGKYFDFVSKCSYVMVDSDCGKGIGFFKLVTENIECGNKGTSCTKSIFFYYRNQVVQLIRGVRPKVMENENYPKDRVKGRISFRDLVSQLIIYTDSDIILYWDKGLSIEIALPPQYQNQVCGLCGNFDQNLNNDFKSKAGNYENNILAFVDSWKSRVNCPAAKDVPDMCKSNHERQAWADKMCGTMKNVKFADCQKVVDTELYYQKCLSDTCSCDRGGDCECLCTALSAFYSACKKYGIDYSWRSNELCPVMCPPEKTFKTCINDCPKKCFKSNLNNTVLENCAKNCVEGCSCPTEYIEAEGSNKCVKENECFCKLPNSDKKYNPGEIFTENCLICVCNENNFKC------------RPDPKCITSTTSYCIGSKLTKCENGVCARICNGIPECSDGSDEANCTSTQTKTLPTTTTQPHCIY-EGRIMQIGEVIKLESCLSVTCTDRRIVETRQNCDKTC-QPNYVLKLY--PDKCCECQLITSTTWTVSHTTTVFEKCEKRPA----------KLTSDSTSVKILNVRGGTEADVANTFTRNADSWIVRNSNASFTLSLPPQAGTELIIVGFQMDITKPFKVVVSFGNEVNKV--ELNPYTVIGQG-KAGKLFQ----TISKDGESFPFVKFT--IESQNNEELEIRNFVLWIC 1669
BLAST of von Willebrand factor vs. Planmine SMEST
Match: SMESG000041532.1 (SMESG000041532.1) HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0 Identity = 482/1311 (36.77%), Postives = 730/1311 (55.68%), Query Frame = 2
Query: 236 SCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLD-NYSGPNCNTLLIKSSQSDVSNYGA-VTRVN-NTMYSTYYECSVIYGHHIKTFDG-NVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT---FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKI--DKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPC-VRDNGES--TYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECV-ERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECL 4129
+C LGW G +C++P+C GC GKC+ PN C C + N G C +GA V V+ N + +C+ + G KTFD G L DC+ P + + C + K+ +DC I++K + ++ V N P++ +S G ++ +KGI DL I +++ +I+V+APVT +K++CGLCG ++G D + + + ++T F+ W L+V RE P A+++ N D + A TC V I K+ ++C E C+ ++ Q +NS C++ ++ + I DWR C++P C N +S+CG+NC CG +++C C PGCYC ++ +W C ++CPC R + + +Y+ G ++K C+ C+C G W+C +++C RC I+GDPH++TFD +H F +G C+Y AVM S + LSI ++N C ++ + C + I ++ GE +Q I + S +E+ +N +++P + ++ + +V++D L++++ + SRIE+ LS KY KVTG+CGNFNG+ DD++ G KD + ++ TC + Y G C S + AN+VC K + + FK C + +DKF QC HDVC CG+ RN + +CEC A+S+YAR C+++ I WRT E CP CP +NM Y +CGS C++TCRL Q C +CI+GC CP+ +E CVK+ DCKCE+ GR Y E R+DRCN CTC NG W CT+ DC + +CP W DCG CQ+TC +++L+C C+ PGC+C V N +CI ECPC+ N+ ++E+ I RGP CQ C C+ GKW C + + C G+CK WGE+HY SFDGK+F+F G CSYV+ ED+C K GTFK++ EN+ CGS +SCTKSI+F Y + + +RG+ PI+ RN + P T + D + ++ T E ++++WD+ +S+++ + +Y+N VCGLCGNFD +DND+R R+G I+ D+ FADSWK+ +SCP P+ C RE WA +C ILKS++F+ CH+ V + YY +CV D+C CDRGDDC+C CTA+ QYV CN Y I ++WR ENCP C R +K C +CP++CI ++D C+EGC+C + + +++ECV E+ C C D + +Y G VI+ C +C C F C K CL
Sbjct: 34 ACCLGWSGKKCNEPVCEKGC-GPGKCIEPNICDCRETNRFGQQCE------------RFGAEVASVSQNIELTDQAQCTYLNGRSYKTFDRVKTVQTNFTGNMVLVQDCMSRPPTYQIIVIPQESCVN-KSSDDCEITIKFLFKDSIEATYLSSQGVKVGNISYSFPYSKAEFQISDRGHYVYVKGIKDLNIFYNKKGSIFVFAPVTMKKQVCGLCGLYDGNGDDKFETVDASMNP-IETDLDFIKIW-------LSVSRENRMKPDPAIQMVNCDLNEMA--------------TCAKVVSIFGSKFEEICYAEFCQ-MKNQ-KNS---------CELMRDIAFLSSPDNIVSDWRYKLD-CEEPNCPNKMIWSDCGNNCNNRCGFNNDDVDCSKVCTPGCYCRKDFFWYKNQCKRADECPCEYRASAKQIDSYKFGDKMKKGCQNCLCESRGLWKCDTHDQCSSRCIITGDPHFVTFDNQHIFVEGQCQYIAVMPKE-----IESDMPQLSIIVENKFCFNSEDITCAKGIKILFGEGLNQTEIEIKSKTEVIINSKLVTIPSKINKGVEISVFKVTSDGLKIEAGDLFSVFRKDDSRIELVLSAKYARKVTGLCGNFNGDPEDDNIMPDGAPAKDDSELAARYRLTQTCHGEVLRQSYLGLCETSIEWGKIANEVCGKFGQGQ-FKDCEALVPSDKFVTQCKHDVCNCGLLRNASTINCECSAYSAYARICSIKGIQFQWRTPEFCPVICP-KNMVYKECGSSCSATCRLRQRPNCDNDCIDGCQCPDDMVIEEKTGNCVKVEDCKCEYMGRDYNANEERKDRCNTCTCINGLWNCTTKDCSSQSYCPNGKVWKDCGGCQRTCRDMNLICDPNSCE-PGCYCESGEVYDNNKCIPKGECPCFWQNQSFNESAVITRGPFECQLSCTCRSGKWECPK-RDCEGLCKAWGESHYMSFDGKFFDFHGDCSYVLLEDYCNKKSGTFKIVIENVECGSKGTSCTKSIRFYYKDHIIHLIRGAAPIIGRNPTWSPYLGTGIFSKHDYVSSIVIITKE-LKIQWDRQLSVDVFLTPEYKNNVCGLCGNFDDNVDNDLRNRAGIIDADVNAFADSWKLKTSCPYPEISKNPCDINKFRESWAIKRCSILKSETFEPCHNTVRYQLYYDKCVQDSCACDRGDDCLCVCTAISQYVHECNRYNIKIQWRNYENCPIMCPPDREYKPCEKVCPETCIGTKSCISD-------CMEGCYCKNNTYLKDDECVSEKPLCTCKDE-SGKEYPLGTTVIKNCHKCTCQINGFECEKELIHNCL 1278
BLAST of von Willebrand factor vs. Planmine SMEST
Match: SMESG000041532.1 (SMESG000041532.1) HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0 Identity = 482/1311 (36.77%), Postives = 730/1311 (55.68%), Query Frame = 2
Query: 236 SCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLD-NYSGPNCNTLLIKSSQSDVSNYGA-VTRVN-NTMYSTYYECSVIYGHHIKTFDG-NVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT---FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKI--DKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPC-VRDNGES--TYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECV-ERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECL 4129
+C LGW G +C++P+C GC GKC+ PN C C + N G C +GA V V+ N + +C+ + G KTFD G L DC+ P + + C + K+ +DC I++K + ++ V N P++ +S G ++ +KGI DL I +++ +I+V+APVT +K++CGLCG ++G D + + + ++T F+ W L+V RE P A+++ N D + A TC V I K+ ++C E C+ ++ Q +NS C++ ++ + I DWR C++P C N +S+CG+NC CG +++C C PGCYC ++ +W C ++CPC R + + +Y+ G ++K C+ C+C G W+C +++C RC I+GDPH++TFD +H F +G C+Y AVM S + LSI ++N C ++ + C + I ++ GE +Q I + S +E+ +N +++P + ++ + +V++D L++++ + SRIE+ LS KY KVTG+CGNFNG+ DD++ G KD + ++ TC + Y G C S + AN+VC K + + FK C + +DKF QC HDVC CG+ RN + +CEC A+S+YAR C+++ I WRT E CP CP +NM Y +CGS C++TCRL Q C +CI+GC CP+ +E CVK+ DCKCE+ GR Y E R+DRCN CTC NG W CT+ DC + +CP W DCG CQ+TC +++L+C C+ PGC+C V N +CI ECPC+ N+ ++E+ I RGP CQ C C+ GKW C + + C G+CK WGE+HY SFDGK+F+F G CSYV+ ED+C K GTFK++ EN+ CGS +SCTKSI+F Y + + +RG+ PI+ RN + P T + D + ++ T E ++++WD+ +S+++ + +Y+N VCGLCGNFD +DND+R R+G I+ D+ FADSWK+ +SCP P+ C RE WA +C ILKS++F+ CH+ V + YY +CV D+C CDRGDDC+C CTA+ QYV CN Y I ++WR ENCP C R +K C +CP++CI ++D C+EGC+C + + +++ECV E+ C C D + +Y G VI+ C +C C F C K CL
Sbjct: 34 ACCLGWSGKKCNEPVCEKGC-GPGKCIEPNICDCRETNRFGQQCE------------RFGAEVASVSQNIELTDQAQCTYLNGRSYKTFDRVKTVQTNFTGNMVLVQDCMSRPPTYQIIVIPQESCVN-KSSDDCEITIKFLFKDSIEATYLSSQGVKVGNISYSFPYSKAEFQISDRGHYVYVKGIKDLNIFYNKKGSIFVFAPVTMKKQVCGLCGLYDGNGDDKFETVDASMNP-IETDLDFIKIW-------LSVSRENRMKPDPAIQMVNCDLNEMA--------------TCAKVVSIFGSKFEEICYAEFCQ-MKNQ-KNS---------CELMRDIAFLSSPDNIVSDWRYKLD-CEEPNCPNKMIWSDCGNNCNNRCGFNNDDVDCSKVCTPGCYCRKDFFWYKNQCKRADECPCEYRASAKQIDSYKFGDKMKKGCQNCLCESRGLWKCDTHDQCSSRCIITGDPHFVTFDNQHIFVEGQCQYIAVMPKE-----IESDMPQLSIIVENKFCFNSEDITCAKGIKILFGEGLNQTEIEIKSKTEVIINSKLVTIPSKINKGVEISVFKVTSDGLKIEAGDLFSVFRKDDSRIELVLSAKYARKVTGLCGNFNGDPEDDNIMPDGAPAKDDSELAARYRLTQTCHGEVLRQSYLGLCETSIEWGKIANEVCGKFGQGQ-FKDCEALVPSDKFVTQCKHDVCNCGLLRNASTINCECSAYSAYARICSIKGIQFQWRTPEFCPVICP-KNMVYKECGSSCSATCRLRQRPNCDNDCIDGCQCPDDMVIEEKTGNCVKVEDCKCEYMGRDYNANEERKDRCNTCTCINGLWNCTTKDCSSQSYCPNGKVWKDCGGCQRTCRDMNLICDPNSCE-PGCYCESGEVYDNNKCIPKGECPCFWQNQSFNESAVITRGPFECQLSCTCRSGKWECPK-RDCEGLCKAWGESHYMSFDGKFFDFHGDCSYVLLEDYCNKKSGTFKIVIENVECGSKGTSCTKSIRFYYKDHIIHLIRGAAPIIGRNPTWSPYLGTGIFSKHDYVSSIVIITKE-LKIQWDRQLSVDVFLTPEYKNNVCGLCGNFDDNVDNDLRNRAGIIDADVNAFADSWKLKTSCPYPEISKNPCDINKFRESWAIKRCSILKSETFEPCHNTVRYQLYYDKCVQDSCACDRGDDCLCVCTAISQYVHECNRYNIKIQWRNYENCPIMCPPDREYKPCEKVCPETCIGTKSCISD-------CMEGCYCKNNTYLKDDECVSEKPLCTCKDE-SGKEYPLGTTVIKNCHKCTCQINGFECEKELIHNCL 1278
BLAST of von Willebrand factor vs. Planmine SMEST
Match: SMESG000041532.1 (SMESG000041532.1) HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0 Identity = 482/1311 (36.77%), Postives = 730/1311 (55.68%), Query Frame = 2
Query: 236 SCSLGWQGNRCSDPICVGGCSNGGKCVAPNQCLCLD-NYSGPNCNTLLIKSSQSDVSNYGA-VTRVN-NTMYSTYYECSVIYGHHIKTFDG-NVFSYPLRGGYTLFMDCLGAAPLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTIILFPKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIKGIDDLEILFDRDRNIYVYAPVTAEKKICGLCGYFNGIMSDDYRDFNQVIQKNVKT---FVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKI--DKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELECESQCLPGCYCPRNRYWDGTMCVPQEKCPC-VRDNGES--TYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFFDGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVNFVKISLPYQHTGNTKVLIDQVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCECEAFSSYARQCALENINLSWRTNELCPKKCPNENMKYNQCGSRCTSTCRLLQESKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQDRCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLHNGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKWVCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDFCGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSSCPVPQKPPFMCSKRPDREPWAKAKCEILKSDSFKKCHDVVNVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECV-ERKACECVDPITNLKYNTGDKVIRECEECNCYEGAFRCTKLFSKECL 4129
+C LGW G +C++P+C GC GKC+ PN C C + N G C +GA V V+ N + +C+ + G KTFD G L DC+ P + + C + K+ +DC I++K + ++ V N P++ +S G ++ +KGI DL I +++ +I+V+APVT +K++CGLCG ++G D + + + ++T F+ W L+V RE P A+++ N D + A TC V I K+ ++C E C+ ++ Q +NS C++ ++ + I DWR C++P C N +S+CG+NC CG +++C C PGCYC ++ +W C ++CPC R + + +Y+ G ++K C+ C+C G W+C +++C RC I+GDPH++TFD +H F +G C+Y AVM S + LSI ++N C ++ + C + I ++ GE +Q I + S +E+ +N +++P + ++ + +V++D L++++ + SRIE+ LS KY KVTG+CGNFNG+ DD++ G KD + ++ TC + Y G C S + AN+VC K + + FK C + +DKF QC HDVC CG+ RN + +CEC A+S+YAR C+++ I WRT E CP CP +NM Y +CGS C++TCRL Q C +CI+GC CP+ +E CVK+ DCKCE+ GR Y E R+DRCN CTC NG W CT+ DC + +CP W DCG CQ+TC +++L+C C+ PGC+C V N +CI ECPC+ N+ ++E+ I RGP CQ C C+ GKW C + + C G+CK WGE+HY SFDGK+F+F G CSYV+ ED+C K GTFK++ EN+ CGS +SCTKSI+F Y + + +RG+ PI+ RN + P T + D + ++ T E ++++WD+ +S+++ + +Y+N VCGLCGNFD +DND+R R+G I+ D+ FADSWK+ +SCP P+ C RE WA +C ILKS++F+ CH+ V + YY +CV D+C CDRGDDC+C CTA+ QYV CN Y I ++WR ENCP C R +K C +CP++CI ++D C+EGC+C + + +++ECV E+ C C D + +Y G VI+ C +C C F C K CL
Sbjct: 34 ACCLGWSGKKCNEPVCEKGC-GPGKCIEPNICDCRETNRFGQQCE------------RFGAEVASVSQNIELTDQAQCTYLNGRSYKTFDRVKTVQTNFTGNMVLVQDCMSRPPTYQIIVIPQESCVN-KSSDDCEITIKFLFKDSIEATYLSSQGVKVGNISYSFPYSKAEFQISDRGHYVYVKGIKDLNIFYNKKGSIFVFAPVTMKKQVCGLCGLYDGNGDDKFETVDASMNP-IETDLDFIKIW-------LSVSRENRMKPDPAIQMVNCDLNEMA--------------TCAKVVSIFGSKFEEICYAEFCQ-MKNQ-KNS---------CELMRDIAFLSSPDNIVSDWRYKLD-CEEPNCPNKMIWSDCGNNCNNRCGFNNDDVDCSKVCTPGCYCRKDFFWYKNQCKRADECPCEYRASAKQIDSYKFGDKMKKGCQNCLCESRGLWKCDTHDQCSSRCIITGDPHFVTFDNQHIFVEGQCQYIAVMPKE-----IESDMPQLSIIVENKFCFNSEDITCAKGIKILFGEGLNQTEIEIKSKTEVIINSKLVTIPSKINKGVEISVFKVTSDGLKIEAGDLFSVFRKDDSRIELVLSAKYARKVTGLCGNFNGDPEDDNIMPDGAPAKDDSELAARYRLTQTCHGEVLRQSYLGLCETSIEWGKIANEVCGKFGQGQ-FKDCEALVPSDKFVTQCKHDVCNCGLLRNASTINCECSAYSAYARICSIKGIQFQWRTPEFCPVICP-KNMVYKECGSSCSATCRLRQRPNCDNDCIDGCQCPDDMVIEEKTGNCVKVEDCKCEYMGRDYNANEERKDRCNTCTCINGLWNCTTKDCSSQSYCPNGKVWKDCGGCQRTCRDMNLICDPNSCE-PGCYCESGEVYDNNKCIPKGECPCFWQNQSFNESAVITRGPFECQLSCTCRSGKWECPK-RDCEGLCKAWGESHYMSFDGKFFDFHGDCSYVLLEDYCNKKSGTFKIVIENVECGSKGTSCTKSIRFYYKDHIIHLIRGAAPIIGRNPTWSPYLGTGIFSKHDYVSSIVIITKE-LKIQWDRQLSVDVFLTPEYKNNVCGLCGNFDDNVDNDLRNRAGIIDADVNAFADSWKLKTSCPYPEISKNPCDINKFRESWAIKRCSILKSETFEPCHNTVRYQLYYDKCVQDSCACDRGDDCLCVCTAISQYVHECNRYNIKIQWRNYENCPIMCPPDREYKPCEKVCPETCIGTKSCISD-------CMEGCYCKNNTYLKDDECVSEKPLCTCKDE-SGKEYPLGTTVIKNCHKCTCQINGFECEKELIHNCL 1278
BLAST of von Willebrand factor vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 369.007 bits (946), Expect = 1.355e-102 Identity = 384/1457 (26.36%), Postives = 597/1457 (40.97%), Query Frame = 2
Query: 287 GGCSNGGKCVAPNQCLCLDNYSGPNC---NTLLIKSS--------QSDV--------------------------------------SNYGAVTRVNNTMYSTYYECSVIYG-HHIKTFDGNVFSYPLRGGYTLFMDCLGAA---PLFYVYFNASYDCFDQKTQNDCSISMKITVGTQTI------ILF------PKNEFALVKNNKTKIPFNSEGIIVSSVGSFIKIK-GIDDLEILFDRDRNIYVYAPVTA-EKKICGLCGYFNGIMSDDYRDFNQVIQKNVKTFVNSWIDRDPSDLNVYREREQYPCNALKLKNNDQYQKALTLCQDMVKNNLGRTCQSEVKIDKYLDMCQDEICECLQTQPENSTIENCYSTFCKIEEQYSYACTLKGIAVDWRSATTICKQPQCFNGQEFSECGSNCGKNCGQLGMELE--CESQCLPGCYCPRNRYWDGTMCVPQEKCPCVRDNGESTYQQGYRLKLNCETCICGMHGKWQCIENEECEGRCFISGDPHYITFDRRHFFF-DGHCEYTAVMTTSDNVLLSNSTIAPLSITIQNTNCQDNSETGCPRTIGVIIGEDEDQVAINLLSSSEIKVN----FVKISLPYQHTGNTKVLID--------------------QVSTDILRLKSETGVEILWNGGSRIEIKLSKKYREKVTGMCGNFNGNEMDDSMTFKGVDEKDIDKFVLSWKTNPTCESDRGIVEYKGACALSRSYSDYANKVCHKLYESEEFKKCHSAANADKFFDQCTHDVCTCGMKRNGALDHCE-CEAFSSYARQCALENIN--LSWRTNEL--CPKKCPNENMKYNQCGSRCTSTCRLLQE---SKCKEECIEGCFCPEGETFDEAAKECVKISDCKCEFHGRFYLPYEIRQD-----RCNNCTCTNGNWICTSNDCEGKEFCPKSMAWFDCGICQKTCTNLDLVCPKGQCQLPGCFCPENTVLH-NGECIKPFECPCYMNNKMYSENDTIVRGPANCQNPCICKDGKW--VCEQNKQCSGICKTWGETHYESFDGKYFEFQGSCSYVISEDF--CGNKLGTFKLISENIACGXXXXXCTKSIKFIYLNRVFQFVRGSNPIVSRNTNYPPGGPTAFYWTSDVDTHFILFTMEGIQVKWDKAMSLEIMIPEKYQNKVCGLCGNFDLRIDNDMRARSGEIEKDIVNFADSWKVHSS-CP---------VPQKPPFMCSKRPDREPWAKAKCEILKSDS--FKKCHDVV--NVEHYYKRCVADACECDRGDDCVCFCTAVEQYVARCNEYEIGLKWRTNENCPHTCTGKRVWKNCGNICPDSCIDIGKNVTDNTGCKKVCIEGCFCPDGLHWQNNECVERKACECVDPITNLKYNTGDKVIRE-CEECNCYEGAFRCTKLFSKECLHTSRPEISTKPPHCVNEYEFLCPNNFC---ARRCNGIIECRDGEDEDLC 4267
GGC NGG C++PNQC C +NY+G C N ++I+S QSD +N+ VNN ++T + YG + +TFD +++Y R Y L +A + VY N + D C + I G + IL+ P + V N + + + V +FI + ++++ +D + NI++ + + GLCG + S+D+ +F+ NV +F NS+ D+ N L D A C + G C V D Y MC IC Q I + C YS +C+ KG + WRS T +C+ QC + +S+C S C C L C++ + C C G C+ E CPC+ D E + G + +C TC C W C E + C C I G + TFD F F D C+Y AV + +N A + + IQ+ N+E+ ++G + + IN +KVN +K + P T V ID +++ +K + ++ ++G I IK+S + + G+CG F+GN +D + I S+ T + + IV+ N +C + EF C +++ DK F T+ C +++ D C A + A C I + + +N L C C + C C STCR L E SKC ++C GC CP + +CV S+C C R P EI C C C NG C + D CPK+ + D +CTN D C C CP +TV +G C++P +CPC N+ Y+ ND I A IC + W + E+ C +C +G+ HY++ DG+ F FQGSC Y + + N + + NIACG S ++C K ++ I + + RG+N + Y ++T V G++V WD++ + + + +Q V GLCGN+D + N+ + + E D + FA+S+ ++ CP P K MC+ PDR+ WA+ C ++K + F C + + +V YY C+ DAC C+RG DC C CT++ + +C I +KWRT CP C G + + C + CP +C IG N + C+EGC CP+G+ N ECV C C Y G + E C++C C + F CT+ +S +C+ T P +F C + C ++ C+ + +C + EDE C
Sbjct: 151 GGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHS----YGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDT-------GCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLS-VTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFD-----NVASFGNSFKGFGCPDI----------ANTLSTCKPDDQILANQQCSKIRLKFPG--CADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDT-LCET-QCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQE--HPPGTVRQFDCNTCTCN-SSLWHCTE-KICPSTCGIMGT-QFDTFDGAKFAFNDVGCQYVAVEPSVNNT----DDRANVFVIIQS-----NTES--------MLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAG----------DNDICSTILNGPEFSGCMNSSKVDKEF-YATY----CRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNY-YKTGTGQCVSESECTCWNPSRPKDP-EIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYD-TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKY----ICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSI------GLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTH-CVEGCSCPNGMIDLNGECVPPTTCPCF--YNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYS-DCITTCTPS------------QFRCDDGKCIESSKICDKVTDCSNSEDEKNC 1509 The following BLAST results are available for this feature:
BLAST of von Willebrand factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of von Willebrand factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of von Willebrand factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of von Willebrand factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of von Willebrand factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of von Willebrand factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009111 ID=SMED30009111|Name=von Willebrand factor|organism=Schmidtea mediterranea sexual|type=transcript|length=5530bpback to top protein sequence of SMED30009111-orf-1 >SMED30009111-orf-1 ID=SMED30009111-orf-1|Name=SMED30009111-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1787bp MGVYFGPQVLVVIIGFCLTTNFQVSKGFLFTKSCSPDCVNGVCKDGRCSCback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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