SMED30009104
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009104 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Alignments
SMED30009104 aligns in the following genomic locations:
Homology
BLAST of SMED30009104 vs. Planmine SMEST
Match: SMESG000023234.1 (SMESG000023234.1) HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0 Identity = 481/486 (98.97%), Postives = 482/486 (99.18%), Query Frame = 2 Query: 113 MISNRYSLFVS--TFFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVICNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAVRNDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQXXXXXXXXXXXXNFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI 1564 MISNRYSLFVS FFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTV+CNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAV NDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQIIFIVILLIMIINFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI Sbjct: 1 MISNRYSLFVSNLAFFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVMCNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAVHNDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQIIFIVILLIMIINFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI 486
BLAST of SMED30009104 vs. Planmine SMEST
Match: SMESG000023234.1 (SMESG000023234.1) HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0 Identity = 416/418 (99.52%), Postives = 417/418 (99.76%), Query Frame = 2 Query: 311 MLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVICNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAVRNDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQXXXXXXXXXXXXNFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI 1564 MLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTV+CNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAV NDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQIIFIVILLIMIINFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI Sbjct: 1 MLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVMCNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIGGKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAVHNDSTGELKKEMLKEVEDYKNILSLDFGSTRYLKISSGNCIYWHEKNEISEPILINVGKSRYNQCVQYEGPYEFQYDSSLFEIQKERVVARNFRDIIENLVKKLEYQIIFIVILLIMIINFTWILCKSRRPKLQKQESVSNIYENQFGLEIASLSLEVPRNQIDSGIYETLPDI 418
BLAST of SMED30009104 vs. Planmine SMEST
Match: SMESG000023216.1 (SMESG000023216.1) HSP 1 Score: 178.718 bits (452), Expect = 1.465e-49 Identity = 118/398 (29.65%), Postives = 202/398 (50.75%), Query Frame = 2 Query: 128 YSLFVSTFFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVICNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIG--GKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAV-RNDSTGELKKEMLKEVE-DYKNILSLDFGSTRYL-KISSGNCIYWHEKNEISEPILINVGKSRYNQ-CVQYEGPYEFQYDSSLFEIQKER 1303 +S+ + L +S+ IYHELNG + S+ N S++ +F+G +++A + + +KL + + ++ R C + +L ++ I + C N ++ I + + H FP K+Y K IWM NIG + GY ++Y ++QL GC+EYFY N+ I +K+ + C S ++TE + T R R++ ++CSL +N C +L Y + Q+Y R G NKT++++ WKQ + K++CL+E+K++ G TI FTV+ I + + D++ + ++L + D N LS+ FG + ++ K+ +G IY + + PI I+VGKSR + C +EG FQYDSS F + R Sbjct: 14 WSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHPGNQNGTIALYYTASRT-----RYTTNCTIEPINHLTVLISLGSGIIILNCKNAMNL------ISSVIDEKDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDPIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYG-TITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNR 399
BLAST of SMED30009104 vs. Planmine SMEST
Match: SMESG000023216.1 (SMESG000023216.1) HSP 1 Score: 176.792 bits (447), Expect = 8.404e-49 Identity = 116/386 (30.05%), Postives = 197/386 (51.04%), Query Frame = 2 Query: 164 LRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVICNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIG--GKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDTNFQIFLAV-RNDSTGELKKEMLKEVE-DYKNILSLDFGSTRYL-KISSGNCIYWHEKNEISEPILINVGKSRYNQ-CVQYEGPYEFQYDSSLFEIQKER 1303 L +S+ IYHELNG + S+ N S++ +F+G +++A + + +KL + + ++ R C + +L ++ I + C N ++ ++ H FP K+Y K IWM NIG + GY ++Y ++QL GC+EYFY N+ I +K+ + C S ++TE + T R R++ ++CSL +N C +L Y + Q+Y R G NKT++++ WKQ + K++CL+E+K++ G TI FTV+ I + + D++ + ++L + D N LS+ FG + ++ K+ +G IY + + PI I+VGKSR + C +EG FQYDSS F + R Sbjct: 26 LPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHPGNQNGTIALYYTASRT-----RYTTNCTIEPINHLTVLISLGSGIIILNCKNAMNLISSVIDENL-------HKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDPIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYG-TITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNR 398
BLAST of SMED30009104 vs. Planmine SMEST
Match: SMESG000023223.1 (SMESG000023223.1) HSP 1 Score: 173.711 bits (439), Expect = 5.403e-48 Identity = 120/398 (30.15%), Postives = 200/398 (50.25%), Query Frame = 2 Query: 128 YSLFVSTFFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTVIFARDFRDKFHLKLEMLWKTKAKVILFSNDRVGSLEIESLCGCEKCSVKNLKFILLFSKQIFTVICNNRQSMKIQLLAIGAGSHQVVYHNFPKSKNYGTNQTKIDIWMANIGQFKGYKGINYYGFREQLKGCLEYFYHYNKSINIKSGEDQFTSPCPSVSLTEIIDYGTRRIERTVTASSFNCSLSVVNKTYCKIEDLHTYNSTYQTYQRIG--GKNKTVKLTFKWKQNFRKNYCLLELKMKDGSTILFTVDT-NFQIFLAVRNDSTGELKKEMLKEVE-DYKNILSLDFGSTRYL-KISSGNCIYWHEKNEISEPILINVGKSRYNQ-CVQYEGPYEFQYDSSLFEIQKER 1303 +S+ + L +S+ IYHELNG + S+ N S++ +F G +++A + + +KL + + ++ R + C E + +L S +I N + +M + I H FP K+Y K IWM NIG + GY ++Y ++QL GC+EYFY N+ I +K+ + C S ++TE + T R R++ ++CSL +N C +L Y + Q+Y R G NKT++++ WKQ + K++CL+E+K++ G TI FTV+ N + D++ + ++L + D N LS+ FG + ++ K+ +G IY + + PI I+VGKSR + C +EG FQYDSS F + R Sbjct: 14 WSVLAAIILFSPLSSTSDYIYHELNGNLTYSKWNLSLFTKNFGGILLYATNANESLIIKLHSGNQNGTIALYYTASRT---RYTTHCTTEPIN----HLTVLISLGSGEMILNCKNAMNLTSSVIDEND----LHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINVNRCDQIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYG-TITFTVNNINAICMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNR 399 The following BLAST results are available for this feature:
BLAST of SMED30009104 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009104 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009104 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009104 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009104 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009104 ID=SMED30009104|Name=SMED30009104|organism=Schmidtea mediterranea sexual|type=transcript|length=1668bpback to top protein sequence of SMED30009104-orf-1 >SMED30009104-orf-1 ID=SMED30009104-orf-1|Name=SMED30009104-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=485bp MISNRYSLFVSTFFNLLLRYSSNVIYHELNGLIHNSELNFSIYLDDFQGTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|