SMED30009087

Overview
NameSMED30009087
Smed IDSMED30009087
Length (bp)1043
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009087 (SMED30009087) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009087 (SMED30009087) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009087

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30009087SMESG000023687.1 dd_Smed_v4_1016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009087SMESG000023687.1 dd_Smed_v4_1016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009087SMESG000023687.1 dd_Smed_v4_1016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009087SMESG000023687.1 dd_Smed_v4_1016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30009087SMESG000023687.1 dd_Smed_v4_1016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009087 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000014:357559..358682 -4993
Homology
[Close]
BLAST of SMED30009087 vs. Ensembl Human
Match: TMEM258 (transmembrane protein 258 [Source:HGNC Symbol;Acc:HGNC:1164])

HSP 1 Score: 131.724 bits (330), Expect = 6.272e-38
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Human
Match: TMEM258 (transmembrane protein 258 [Source:HGNC Symbol;Acc:HGNC:1164])

HSP 1 Score: 131.724 bits (330), Expect = 6.272e-38
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Human
Match: TMEM258 (transmembrane protein 258 [Source:HGNC Symbol;Acc:HGNC:1164])

HSP 1 Score: 128.257 bits (321), Expect = 1.508e-36
Identity = 59/74 (79.73%), Postives = 72/74 (97.30%), Query Frame = -2 
Query:  740 MTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 961
            M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 74
[Close]
BLAST of SMED30009087 vs. Ensembl Human
Match: TMEM258 (transmembrane protein 258 [Source:HGNC Symbol;Acc:HGNC:1164])

HSP 1 Score: 67.0106 bits (162), Expect = 6.649e-14
Identity = 29/42 (69.05%), Postives = 36/42 (85.71%), Query Frame = -2 
Query:  851 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVT 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVY  T
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYPFT 42
[Close]
BLAST of SMED30009087 vs. Ensembl Human
Match: TMEM258 (transmembrane protein 258 [Source:HGNC Symbol;Acc:HGNC:1164])

HSP 1 Score: 67.0106 bits (162), Expect = 6.649e-14
Identity = 29/42 (69.05%), Postives = 36/42 (85.71%), Query Frame = -2 
Query:  851 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVT 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVY  T
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYPFT 42
[Close]
BLAST of SMED30009087 vs. Ensembl Celegans
Match: Y57E12AM.1 (Transmembrane protein 258 [Source:UniProtKB/Swiss-Prot;Acc:Q965T1])

HSP 1 Score: 85.8853 bits (211), Expect = 5.547e-21
Identity = 40/79 (50.63%), Postives = 55/79 (69.62%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + I  M RY +PVN +  P LT  L  +G+  +A F + +VT+TKY R+L KEL +A  +SVF+GFG +FL+LWVGIYV
Sbjct:    1 MDISKMNRYTAPVNFASLPLLTTFLCGVGLLLLATFTMIQVTSTKYNRNLLKELFIAATSSVFLGFGSVFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Fly
Match: kud (gene:FBgn0036667 transcript:FBtr0075339)

HSP 1 Score: 91.6633 bits (226), Expect = 5.893e-23
Identity = 45/79 (56.96%), Postives = 63/79 (79.75%), Query Frame = -2 
Query:  740 IEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRD--LFKELVVALVASVFMGFGVLFLMLWVGIYV 970
            ++ M RYVSPVNP+V+PHL  VLL IG FF AWFF++ V + K +++  L KEL+++L AS+F+GFG++FL+L VGIYV
Sbjct:    1 MDVMQRYVSPVNPAVFPHLATVLLVIGTFFTAWFFIF-VVSRKSSKESTLIKELLISLCASIFLGFGIVFLLLTVGIYV 78
[Close]
BLAST of SMED30009087 vs. Ensembl Fly
Match: kud (gene:FBgn0036667 transcript:FBtr0305272)

HSP 1 Score: 91.6633 bits (226), Expect = 5.893e-23
Identity = 45/79 (56.96%), Postives = 63/79 (79.75%), Query Frame = -2 
Query:  740 IEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRD--LFKELVVALVASVFMGFGVLFLMLWVGIYV 970
            ++ M RYVSPVNP+V+PHL  VLL IG FF AWFF++ V + K +++  L KEL+++L AS+F+GFG++FL+L VGIYV
Sbjct:    1 MDVMQRYVSPVNPAVFPHLATVLLVIGTFFTAWFFIF-VVSRKSSKESTLIKELLISLCASIFLGFGIVFLLLTVGIYV 78
[Close]
BLAST of SMED30009087 vs. Ensembl Zebrafish
Match: tmem258 (transmembrane protein 258 [Source:NCBI gene;Acc:393742])

HSP 1 Score: 129.798 bits (325), Expect = 2.390e-37
Identity = 60/79 (75.95%), Postives = 74/79 (93.67%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E MTRY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL++ALVAS+FMGFGV FL+LWVGI+V
Sbjct:    1 MELEAMTRYTSPVNPAVFPHLTVVLLAIGMFFKAWFFVYEVTSTKYTRDVYKELLIALVASLFMGFGVHFLLLWVGIFV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Xenopus
Match: rab30 (RAB30, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-479337])

HSP 1 Score: 131.339 bits (329), Expect = 8.345e-38
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Xenopus
Match: rab30 (RAB30, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-479337])

HSP 1 Score: 131.339 bits (329), Expect = 1.033e-37
Identity = 60/78 (76.92%), Postives = 74/78 (94.87%), Query Frame = -2 
Query:  740 SIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 973
             +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    2 ELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Mouse
Match: Tmem258 (transmembrane protein 258 [Source:MGI Symbol;Acc:MGI:1916288])

HSP 1 Score: 131.724 bits (330), Expect = 4.306e-38
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Ensembl Mouse
Match: Tmem258 (transmembrane protein 258 [Source:MGI Symbol;Acc:MGI:1916288])

HSP 1 Score: 128.257 bits (321), Expect = 1.036e-36
Identity = 59/74 (79.73%), Postives = 72/74 (97.30%), Query Frame = -2 
Query:  740 MTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 961
            M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 74
[Close]
BLAST of SMED30009087 vs. UniProt/SwissProt
Match: sp|P61166|TM258_MOUSE (Transmembrane protein 258 OS=Mus musculus OX=10090 GN=Tmem258 PE=1 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.012e-37
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. UniProt/SwissProt
Match: sp|P61165|TM258_HUMAN (Transmembrane protein 258 OS=Homo sapiens OX=9606 GN=TMEM258 PE=1 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.012e-37
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. UniProt/SwissProt
Match: sp|Q76LT9|TM258_CHICK (Transmembrane protein 258 OS=Gallus gallus OX=9031 GN=TMEM258 PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.012e-37
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. UniProt/SwissProt
Match: sp|Q32P84|TM258_BOVIN (Transmembrane protein 258 OS=Bos taurus OX=9913 GN=TMEM258 PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.890e-37
Identity = 59/79 (74.68%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIY+
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYI 79
[Close]
BLAST of SMED30009087 vs. UniProt/SwissProt
Match: sp|Q6DDB3|TM258_XENTR (Transmembrane protein 258 OS=Xenopus tropicalis OX=8364 GN=tmem258 PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.421e-37
Identity = 60/79 (75.95%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E M+RY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. TrEMBL
Match: V3ZVL9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_196219 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 8.306e-37
Identity = 64/79 (81.01%), Postives = 75/79 (94.94%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + IE M+RYVSP+NP+V+PHLTVVLL IGIFFMAWFFVYEVTATK+TRD+FKEL+V+L ASVFMGFGV+FL+LWVGIYV
Sbjct:    2 VGIESMSRYVSPINPAVFPHLTVVLLGIGIFFMAWFFVYEVTATKFTRDIFKELLVSLFASVFMGFGVMFLLLWVGIYV 80
[Close]
BLAST of SMED30009087 vs. TrEMBL
Match: A0A3M6U4U8 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00019601 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.689e-36
Identity = 62/79 (78.48%), Postives = 76/79 (96.20%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            +S++ M RY SP+NP+V+PHLTVVLLSIGIFFMAWFFVYEVT+TKY+RD++KEL+V+LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    4 LSVDQMNRYASPINPAVFPHLTVVLLSIGIFFMAWFFVYEVTSTKYSRDIYKELLVSLVASLFMGFGVLFLLLWVGIYV 82
[Close]
BLAST of SMED30009087 vs. TrEMBL
Match: A0A060YSQ8 (Uncharacterized protein (Fragment) OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00004878001 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.081e-36
Identity = 65/88 (73.86%), Postives = 79/88 (89.77%), Query Frame = -2 
Query:  740 SNSEKFRMEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 1003
            S  +  +ME+  E MTRY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD+FKEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    3 SKQQLNKMEL--EAMTRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVFKELLISLVASLFMGFGVLFLLLWVGIYV 88
[Close]
BLAST of SMED30009087 vs. TrEMBL
Match: A0A0S7I6K8 (TM258 (Fragment) OS=Poeciliopsis prolifica OX=188132 GN=TM258 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.476e-36
Identity = 62/82 (75.61%), Postives = 77/82 (93.90%), Query Frame = -2 
Query:  740 RMEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 985
            R ++ +E MTRY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:   27 RHKMELEAMTRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVYKELLISLVASLFMGFGVLFLLLWVGIYV 108
[Close]
BLAST of SMED30009087 vs. TrEMBL
Match: A0A267FY93 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006314g6 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.499e-36
Identity = 60/82 (73.17%), Postives = 76/82 (92.68%), Query Frame = -2 
Query:  740 RMEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 985
            +MEI+++ M RYVSP+NP+V+PHLT+VLL IGIFF AWFFVYEVTA K++RD+FKE++++LVASVFMGFG+LFL LWVGIYV
Sbjct:    2 KMEIALDSMVRYVSPINPAVFPHLTLVLLGIGIFFTAWFFVYEVTANKFSRDIFKEILISLVASVFMGFGILFLCLWVGIYV 83
[Close]
BLAST of SMED30009087 vs. Ensembl Cavefish
Match: tmem258 (transmembrane protein 258 [Source:NCBI gene;Acc:393742])

HSP 1 Score: 129.028 bits (323), Expect = 3.699e-37
Identity = 60/74 (81.08%), Postives = 72/74 (97.30%), Query Frame = -2 
Query:  740 MTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 961
            MTRY SPVNP+V+PHLTVVLL+IG+FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MTRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDVYKELLISLVASLFMGFGVLFLLLWVGIYV 74
[Close]
BLAST of SMED30009087 vs. Ensembl Nematostella
Match: EDO32034 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW81])

HSP 1 Score: 126.331 bits (316), Expect = 1.374e-36
Identity = 61/77 (79.22%), Postives = 73/77 (94.81%), Query Frame = -2 
Query:  740 MTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVY---EVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 961
            M+RY SP+NP+V+PHLTVVLL+IG+FFMAWFFVY   EVT+TKYTRD++KEL+VALVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MSRYASPINPAVFPHLTVVLLAIGVFFMAWFFVYPFYEVTSTKYTRDIYKELLVALVASLFMGFGVLFLLLWVGIYV 77
[Close]
BLAST of SMED30009087 vs. Ensembl Medaka
Match: tmem258 (transmembrane protein 258 [Source:NCBI gene;Acc:105354217])

HSP 1 Score: 132.494 bits (332), Expect = 1.628e-38
Identity = 61/79 (77.22%), Postives = 74/79 (93.67%), Query Frame = -2 
Query:  740 ISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 976
            + +E MTRY SPVNP+V+PHLTVVLL+IG FF AWFFVYEVT+TKYTRD++KEL+++LVAS+FMGFGVLFL+LWVGIYV
Sbjct:    1 MELEAMTRYTSPVNPAVFPHLTVVLLAIGTFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV 79
[Close]
BLAST of SMED30009087 vs. Planmine SMEST
Match: SMESG000023687.1 (SMESG000023687.1)

HSP 1 Score: 161.384 bits (407), Expect = 1.126e-49
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = -2 
Query:  740 MEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 982
            MEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV
Sbjct:    1 MEISIEDMTRYVSPVNPSVYPHLTVVLLSIGIFFMAWFFVYEVTATKYTRDLFKELVVALVASVFMGFGVLFLMLWVGIYV 81
The following BLAST results are available for this feature:
BLAST of SMED30009087 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMEM2586.272e-3875.95transmembrane protein 258 [Source:HGNC Symbol;Acc:... [more]
TMEM2586.272e-3875.95transmembrane protein 258 [Source:HGNC Symbol;Acc:... [more]
TMEM2581.508e-3679.73transmembrane protein 258 [Source:HGNC Symbol;Acc:... [more]
TMEM2586.649e-1469.05transmembrane protein 258 [Source:HGNC Symbol;Acc:... [more]
TMEM2586.649e-1469.05transmembrane protein 258 [Source:HGNC Symbol;Acc:... [more]
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BLAST of SMED30009087 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y57E12AM.15.547e-2150.63Transmembrane protein 258 [Source:UniProtKB/Swiss... [more]
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BLAST of SMED30009087 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
kud5.893e-2356.96gene:FBgn0036667 transcript:FBtr0075339[more]
kud5.893e-2356.96gene:FBgn0036667 transcript:FBtr0305272[more]
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BLAST of SMED30009087 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2582.390e-3775.95transmembrane protein 258 [Source:NCBI gene;Acc:39... [more]
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BLAST of SMED30009087 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rab308.345e-3875.95RAB30, member RAS oncogene family [Source:Xenbase;... [more]
rab301.033e-3776.92RAB30, member RAS oncogene family [Source:Xenbase;... [more]
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BLAST of SMED30009087 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tmem2584.306e-3875.95transmembrane protein 258 [Source:MGI Symbol;Acc:M... [more]
Tmem2581.036e-3679.73transmembrane protein 258 [Source:MGI Symbol;Acc:M... [more]
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BLAST of SMED30009087 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P61166|TM258_MOUSE3.012e-3775.95Transmembrane protein 258 OS=Mus musculus OX=10090... [more]
sp|P61165|TM258_HUMAN3.012e-3775.95Transmembrane protein 258 OS=Homo sapiens OX=9606 ... [more]
sp|Q76LT9|TM258_CHICK3.012e-3775.95Transmembrane protein 258 OS=Gallus gallus OX=9031... [more]
sp|Q32P84|TM258_BOVIN3.890e-3774.68Transmembrane protein 258 OS=Bos taurus OX=9913 GN... [more]
sp|Q6DDB3|TM258_XENTR4.421e-3775.95Transmembrane protein 258 OS=Xenopus tropicalis OX... [more]
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BLAST of SMED30009087 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V3ZVL98.306e-3781.01Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A3M6U4U81.689e-3678.48Uncharacterized protein OS=Pocillopora damicornis ... [more]
A0A060YSQ82.081e-3673.86Uncharacterized protein (Fragment) OS=Oncorhynchus... [more]
A0A0S7I6K82.476e-3675.61TM258 (Fragment) OS=Poeciliopsis prolifica OX=1881... [more]
A0A267FY934.499e-3673.17Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30009087 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2583.699e-3781.08transmembrane protein 258 [Source:NCBI gene;Acc:39... [more]
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BLAST of SMED30009087 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009087 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009087 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO320341.374e-3679.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30009087 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2581.628e-3877.22transmembrane protein 258 [Source:NCBI gene;Acc:10... [more]
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BLAST of SMED30009087 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000023687.11.126e-49100.00SMESG000023687.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009087 ID=SMED30009087|Name=SMED30009087|organism=Schmidtea mediterranea sexual|type=transcript|length=1043bp
TTTCAAGGTTAAGTTAGGCGCAAATGCGAATAAGATTTTGCATCCAAATT
ATTGCTGATCTAAACTAGGTTGGAATAGACTTCTTGTGAACAAAGGAAAT
ATCAGGATAGTGGAAACAGATTGGATACTTGTAATCCTGGTTCATTGAAT
TGGCAAACAAGAGTCAANCCCGGATTAATGCAGTAGTCAAATGATTTATC
TACCTTCATAAAATAATATTCGATTATGTGACTGGACACTAGACCAACTT
CAAATATATATTAGCAAAATTTTCAACACTTTCGAACGTACCAAGTTCAT
CTCTAATAAGAACGTAATTACGAACAAAAAATTACAAAAAACAAATTATC
AGCACACCAAAGACAGTAATCCTGCAACCCATGATGCTATATTTCAAAAT
TCCTTATTTTAATAAAATCGAAATTTCAAATATCTATCATACTGAAATAA
ATTAGGATTGTTTTATGATTATTNGGTTTAGAAAAAATAAATTCACTCAT
AAAAGCACAACGGAATCACATTTACAAAAAANTTTTCAAAGATTAGCATA
ATTTCAAAAGTATAGACATATTTTTTTCATAAACATTTACAATATTCAAA
TGGATAAACAATGTTGAAAAGCAATAAGCTTCAACTCAATTGTACCTTAA
ATAAGCACTCGAAATAATTCAAATATCCATTTATATTTAAATTACAAAAA
ACAAATTTTATTTGAAAAACACAATTTCCATAAATACTAAACATATATTC
CGACCCAAAGCATCAAAAATAAAACACCAAATCCCATAAATACAGATGCA
ACTAGTGCAACAACGAGTTCCTTAAACAGATCACGAGTGTATTTTGTAGC
TGTGACCTCATAAACAAAAAACCAGGCCATAAAAAAAATGCCAATTGATA
GTAAAACAACAGTTAAATGCGGATAAACCGATGGATTAACTGGTGAAACG
TATCTCGTCATGTCTTCGATAGAAATCTCCATTCTAAATTTTTCAGAATT
TGAATAAATGATGAAGACGTAATTCACTTAAAGNNANNGNAAN
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
Vocabulary: biological process
TermDefinition
GO:0006487protein N-linked glycosylation
Vocabulary: cellular component
TermDefinition
GO:0034998oligosaccharyltransferase I complex
Vocabulary: INTERPRO
TermDefinition
IPR007915TMEM258/Ost5
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007915Oligosaccharyltransferase complex subunitPFAMPF05251Ost5coord: 10..81
e-value: 6.3E-32
score: 109.7
IPR007915Oligosaccharyltransferase complex subunitPANTHERPTHR13636UNCHARACTERIZEDcoord: 5..81
NoneNo IPR availablePANTHERPTHR13636:SF0TRANSMEMBRANE PROTEIN 258coord: 5..81
NoneNo IPR availableTMHMMTMhelixcoord: 56..78
NoneNo IPR availableTMHMMTMhelixcoord: 19..41