SMED30009057

Overview
NameSMED30009057
Smed IDSMED30009057
Length (bp)396
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009057

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30009057h1SMcG0020780 PL06001X1A02smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30009057h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30009057h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30009057h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30009057h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009057h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009057h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009057h1SMcG0020780 Contig20101newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009057h1SMcG0020780 Contig20101uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009057 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006308:15230..18087 -1742
Homology
BLAST of SMED30009057 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.5666 bits (192), Expect = 2.424e-17
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+    +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  275 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASID 398          

HSP 2 Score: 76.2554 bits (186), Expect = 1.223e-16
Identity = 41/124 (33.06%), Postives = 66/124 (53.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K  + A   ++  P+H A   GH  +V LL + N N
Sbjct:  374 PLHIACKKNHVRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNAN 495          

HSP 3 Score: 66.6254 bits (161), Expect = 3.314e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  638 PLHLAAQEGHAEMVALLLSKQANGNLGN-KSGLTPLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 4 Score: 65.4698 bits (158), Expect = 7.122e-13
Identity = 39/130 (30.00%), Postives = 66/130 (50.77%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+ +  +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GHAE+V LL     N
Sbjct:  539 PLHVAAKYGKVRVAELLLERDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660          

HSP 5 Score: 65.4698 bits (158), Expect = 7.191e-13
Identity = 33/124 (26.61%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ K L+  K  + A + ++ +  +H A   GH  +VKLL++ + +   +     + P+H A  +GH+E    L+EK+ +          P+H+A   G   +  LL + + +
Sbjct:  440 PLHMAARAGHTEVAKYLLQNKAKVNA-KAKDDQTPLHCAARIGHTNMVKLLLENNAN-PNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAH 561          

HSP 6 Score: 63.1586 bits (152), Expect = 6.049e-12
Identity = 34/128 (26.56%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + K+L++K     + R  N    +H AC++ H+ +++LL+     I+A+       P+H A   GHL I K L+++  +     +    P+H+A   GH E+   L      +  +
Sbjct:  341 PLHVAAHCGHHRVAKVLLDKGAKPNS-RALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLT-PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAK 466          

HSP 7 Score: 60.077 bits (144), Expect = 5.820e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  605 PLHIAAKQNQVEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHAEMVALLLSKQAN-GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 730          

HSP 8 Score: 57.3806 bits (137), Expect = 4.701e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   49 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 165          

HSP 9 Score: 57.3806 bits (137), Expect = 5.279e-10
Identity = 33/120 (27.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L
Sbjct:  572 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVL 687          

HSP 10 Score: 48.9062 bits (115), Expect = 4.213e-7
Identity = 28/118 (23.73%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      +   P+ +A   G+  +  +L
Sbjct:  671 PLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 786          

HSP 11 Score: 48.9062 bits (115), Expect = 4.869e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  114 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETK 268          
BLAST of SMED30009057 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.5666 bits (192), Expect = 2.424e-17
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+    +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  275 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASID 398          

HSP 2 Score: 76.2554 bits (186), Expect = 1.223e-16
Identity = 41/124 (33.06%), Postives = 66/124 (53.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K  + A   ++  P+H A   GH  +V LL + N N
Sbjct:  374 PLHIACKKNHVRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNAN 495          

HSP 3 Score: 66.6254 bits (161), Expect = 3.314e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  638 PLHLAAQEGHAEMVALLLSKQANGNLGN-KSGLTPLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 4 Score: 65.4698 bits (158), Expect = 7.122e-13
Identity = 39/130 (30.00%), Postives = 66/130 (50.77%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+ +  +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GHAE+V LL     N
Sbjct:  539 PLHVAAKYGKVRVAELLLERDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660          

HSP 5 Score: 65.4698 bits (158), Expect = 7.191e-13
Identity = 33/124 (26.61%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ K L+  K  + A + ++ +  +H A   GH  +VKLL++ + +   +     + P+H A  +GH+E    L+EK+ +          P+H+A   G   +  LL + + +
Sbjct:  440 PLHMAARAGHTEVAKYLLQNKAKVNA-KAKDDQTPLHCAARIGHTNMVKLLLENNAN-PNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAH 561          

HSP 6 Score: 63.1586 bits (152), Expect = 6.049e-12
Identity = 34/128 (26.56%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + K+L++K     + R  N    +H AC++ H+ +++LL+     I+A+       P+H A   GHL I K L+++  +     +    P+H+A   GH E+   L      +  +
Sbjct:  341 PLHVAAHCGHHRVAKVLLDKGAKPNS-RALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLT-PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAK 466          

HSP 7 Score: 60.077 bits (144), Expect = 5.821e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  605 PLHIAAKQNQVEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHAEMVALLLSKQAN-GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 730          

HSP 8 Score: 57.3806 bits (137), Expect = 4.701e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   49 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 165          

HSP 9 Score: 57.3806 bits (137), Expect = 5.279e-10
Identity = 33/120 (27.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L
Sbjct:  572 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVL 687          

HSP 10 Score: 48.9062 bits (115), Expect = 4.213e-7
Identity = 28/118 (23.73%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      +   P+ +A   G+  +  +L
Sbjct:  671 PLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 786          

HSP 11 Score: 48.9062 bits (115), Expect = 4.869e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  114 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETK 268          
BLAST of SMED30009057 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.5666 bits (192), Expect = 2.425e-17
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+    +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  308 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASID 431          

HSP 2 Score: 76.2554 bits (186), Expect = 1.223e-16
Identity = 41/124 (33.06%), Postives = 66/124 (53.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K  + A   ++  P+H A   GH  +V LL + N N
Sbjct:  407 PLHIACKKNHVRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNAN 528          

HSP 3 Score: 66.6254 bits (161), Expect = 3.316e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  671 PLHLAAQEGHAEMVALLLSKQANGNLGN-KSGLTPLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 787          

HSP 4 Score: 65.4698 bits (158), Expect = 7.124e-13
Identity = 39/130 (30.00%), Postives = 66/130 (50.77%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+ +  +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GHAE+V LL     N
Sbjct:  572 PLHVAAKYGKVRVAELLLERDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 693          

HSP 5 Score: 65.4698 bits (158), Expect = 7.194e-13
Identity = 33/124 (26.61%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ K L+  K  + A + ++ +  +H A   GH  +VKLL++ + +   +     + P+H A  +GH+E    L+EK+ +          P+H+A   G   +  LL + + +
Sbjct:  473 PLHMAARAGHTEVAKYLLQNKAKVNA-KAKDDQTPLHCAARIGHTNMVKLLLENNAN-PNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAH 594          

HSP 6 Score: 63.1586 bits (152), Expect = 6.051e-12
Identity = 34/128 (26.56%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + K+L++K     + R  N    +H AC++ H+ +++LL+     I+A+       P+H A   GHL I K L+++  +     +    P+H+A   GH E+   L      +  +
Sbjct:  374 PLHVAAHCGHHRVAKVLLDKGAKPNS-RALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLT-PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAK 499          

HSP 7 Score: 60.077 bits (144), Expect = 5.823e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  638 PLHIAAKQNQVEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHAEMVALLLSKQAN-GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 763          

HSP 8 Score: 57.3806 bits (137), Expect = 4.702e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   82 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 198          

HSP 9 Score: 57.3806 bits (137), Expect = 5.281e-10
Identity = 33/120 (27.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L
Sbjct:  605 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVL 720          

HSP 10 Score: 50.8322 bits (120), Expect = 1.180e-7
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   52 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 172          

HSP 11 Score: 48.9062 bits (115), Expect = 4.215e-7
Identity = 28/118 (23.73%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      +   P+ +A   G+  +  +L
Sbjct:  704 PLHLVAQEGHVPVADVLIKHGVMVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 819          

HSP 12 Score: 48.9062 bits (115), Expect = 4.870e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  147 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETK 301          
BLAST of SMED30009057 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 73.9442 bits (180), Expect = 9.818e-16
Identity = 35/126 (27.78%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H   ++GH ++V++L+++   I + + +N    +H A +  HL  V+LL+  +  ++ +   +    +H A   GH ++AKVL++KK N  A  +N   P+H+AC +   +++ LL     +I+
Sbjct:  298 PLHCGARSGHEQVVEMLLDRAAPILS-KTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLT-ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQ 421          

HSP 2 Score: 70.4774 bits (171), Expect = 1.538e-14
Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            A + GHLE     I    +I  C  QN   A+H A ++GH+E+V  L+ +  +++A   K  N  +H A   G  E+ KVL+    N+ A   N   P+++A    H E+V  L D
Sbjct:   42 AARAGHLEKALDYIKNGVDINICN-QNGLNALHLASKEGHVEVVSELLQREANVDA-ATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLD 155          

HSP 3 Score: 66.6254 bits (161), Expect = 3.511e-13
Identity = 31/123 (25.20%), Postives = 64/123 (52.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            +HLA + GH+++V LL+ +  N+     ++    +H A ++  + + ++L++Q   ++A   K    P+H  C  G+++I   L++    + A   N   P+H A  +GH  I+ +L   N +
Sbjct:  662 VHLAAQEGHVDMVSLLLGRNANVNLSN-KSGLTPLHLAAQEDRVNVAEVLVNQGAHVDA-QTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 782          

HSP 4 Score: 66.6254 bits (161), Expect = 3.545e-13
Identity = 36/120 (30.00%), Postives = 68/120 (56.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCD--IEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A +  HL  V+LL+     ++     +   A+H A   GH ++ K+L+D+  +   +A+ G     P+H AC+K  +++ ++L++   +I+A   + L PIH+A + GH  IV+ L
Sbjct:  331 PLHMATQGDHLNCVQLLLQHNVPVDDVT-NDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNG---FTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQL 446          

HSP 5 Score: 66.2402 bits (160), Expect = 4.139e-13
Identity = 36/129 (27.91%), Postives = 72/129 (55.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+E+V  L+ ++ N++A   +    A+H A   G  E+VK+L+    ++ A   + +N   P++ A ++ HLE+ K L++   +      +   P+ +A  +GH ++V+LL + +   +V+
Sbjct:   72 LHLASKEGHVEVVSELLQREANVDAAT-KKGNTALHIASLAGQAEVVKVLVTNGANVNA---QSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR 196          

HSP 6 Score: 64.6994 bits (156), Expect = 1.443e-12
Identity = 38/127 (29.92%), Postives = 64/127 (50.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G LE+  LL+ K  + +A   ++    +H A    + ++  LL+DQ     A   K    P+H A +K  ++IA  L+E   +  A     +  +HLA   GH ++V+LL   N N+ +
Sbjct:  562 PLHVAAKYGKLEVANLLLQKSASPDAAG-KSGLTPLHVAAHYDNQKVALLLLDQGASPHA-AAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNL 686          

HSP 7 Score: 62.003 bits (149), Expect = 1.337e-11
Identity = 30/128 (23.44%), Postives = 67/128 (52.34%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   ++I   L+    +  A   Q    ++H A ++GH+++V LL+ ++ ++  +  K    P+H A ++  + +A+VL+ +  +++A       P+H+ C  G+ +IV  L   +  +  +
Sbjct:  628 PLHIAAKKNQMDIATTLLEYGADANAVTRQGIA-SVHLAAQEGHVDMVSLLLGRNANVN-LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAK 753          

HSP 8 Score: 60.077 bits (144), Expect = 5.437e-11
Identity = 33/127 (25.98%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            PIH+A   GH+ IV  L++   +      +  E A+H A   G  E+V+ L+     +EA   K    P+H +   G  +I + L+++  +  A   +   P+HL+   GH ++   L D   ++ +
Sbjct:  430 PIHVAAFMGHVNIVSQLMHHGASPNTTNVR-GETALHMAARSGQAEVVRYLVQDGAQVEA-KAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSI 554          

HSP 9 Score: 60.077 bits (144), Expect = 5.762e-11
Identity = 37/159 (23.27%), Postives = 74/159 (46.54%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLI-------------------------------DQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L++   + ++   ++    +  A +QGH ++V LL+                               D + D+E+  G     P+H A   G++ +A +L+ +   ++    N++ P+H+A  RG+A +V LL D    I+ +
Sbjct:  137 PLYMAAQENHLEVVKFLLDNGAS-QSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSG---FTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK 291          

HSP 10 Score: 59.6918 bits (143), Expect = 7.482e-11
Identity = 34/127 (26.77%), Postives = 62/127 (48.82%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH ++ K+L++KK N  A +  N    +H AC++  +++++LL+     I+A+       PIH A   GH+ I   L+    +     +     +H+A   G AE+V  L      +E +
Sbjct:  365 LHVAAHCGHYKVAKVLLDKKANPNA-KALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLT-PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAK 489          

HSP 11 Score: 52.7582 bits (125), Expect = 2.352e-8
Identity = 28/118 (23.73%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            +H+A ++G  E+V+ L+     +EA + ++ +  +H +   G  +IV+ L+ Q     A        P+H +  +GH ++A  L++   ++         P+H+A   G  E+  LL 
Sbjct:  464 LHMAARSGQAEVVRYLVQDGAQVEA-KAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 579          

HSP 12 Score: 50.447 bits (119), Expect = 1.458e-7
Identity = 30/120 (25.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA +   + + ++L+N+  +++A + +     +H  C  G+++IV  L+     + A   K KN   P+H A ++GH  I  VL++   +     +N    + +A   G+  +V  L
Sbjct:  694 PLHLAAQEDRVNVAEVLVNQGAHVDA-QTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNA---KTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTL 809          

HSP 13 Score: 49.6766 bits (117), Expect = 2.478e-7
Identity = 26/128 (20.31%), Postives = 63/128 (49.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A    + ++  LL+++  +  A   +N    +H A ++  ++I   L++   D  A+  +     +H A ++GH+++  +L+ +  N+     + L P+HLA       +  +L +   +++ Q
Sbjct:  595 PLHVAAHYDNQKVALLLLDQGASPHAAA-KNGYTPLHIAAKKNQMDIATTLLEYGADANAVT-RQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQ 720          

HSP 14 Score: 48.9062 bits (115), Expect = 5.464e-7
Identity = 26/119 (21.85%), Postives = 59/119 (49.58%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +H+A +    +   LL+    N +    ++    +H A   G++ +  LL+++   ++    +    P+H A ++G+  + K+L+++   I+A   + L P+H     GH ++V +L D
Sbjct:  200 LHIAARKDDTKAAALLLQNDNNADV-ESKSGFTPLHIAAHYGNINVATLLLNRAAAVD-FTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 316          
BLAST of SMED30009057 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 73.9442 bits (180), Expect = 9.820e-16
Identity = 35/126 (27.78%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H   ++GH ++V++L+++   I + + +N    +H A +  HL  V+LL+  +  ++ +   +    +H A   GH ++AKVL++KK N  A  +N   P+H+AC +   +++ LL     +I+
Sbjct:  287 PLHCGARSGHEQVVEMLLDRAAPILS-KTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLT-ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQ 410          

HSP 2 Score: 70.4774 bits (171), Expect = 1.538e-14
Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            A + GHLE     I    +I  C  QN   A+H A ++GH+E+V  L+ +  +++A   K  N  +H A   G  E+ KVL+    N+ A   N   P+++A    H E+V  L D
Sbjct:   31 AARAGHLEKALDYIKNGVDINICN-QNGLNALHLASKEGHVEVVSELLQREANVDA-ATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLD 144          

HSP 3 Score: 66.6254 bits (161), Expect = 3.512e-13
Identity = 31/123 (25.20%), Postives = 64/123 (52.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            +HLA + GH+++V LL+ +  N+     ++    +H A ++  + + ++L++Q   ++A   K    P+H  C  G+++I   L++    + A   N   P+H A  +GH  I+ +L   N +
Sbjct:  651 VHLAAQEGHVDMVSLLLGRNANVNLSN-KSGLTPLHLAAQEDRVNVAEVLVNQGAHVDA-QTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 771          

HSP 4 Score: 66.6254 bits (161), Expect = 3.546e-13
Identity = 36/120 (30.00%), Postives = 68/120 (56.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCD--IEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A +  HL  V+LL+     ++     +   A+H A   GH ++ K+L+D+  +   +A+ G     P+H AC+K  +++ ++L++   +I+A   + L PIH+A + GH  IV+ L
Sbjct:  320 PLHMATQGDHLNCVQLLLQHNVPVDDVT-NDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNG---FTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQL 435          

HSP 5 Score: 66.2402 bits (160), Expect = 4.140e-13
Identity = 36/129 (27.91%), Postives = 72/129 (55.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+E+V  L+ ++ N++A   +    A+H A   G  E+VK+L+    ++ A   + +N   P++ A ++ HLE+ K L++   +      +   P+ +A  +GH ++V+LL + +   +V+
Sbjct:   61 LHLASKEGHVEVVSELLQREANVDAAT-KKGNTALHIASLAGQAEVVKVLVTNGANVNA---QSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR 185          

HSP 6 Score: 64.6994 bits (156), Expect = 1.443e-12
Identity = 38/127 (29.92%), Postives = 64/127 (50.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G LE+  LL+ K  + +A   ++    +H A    + ++  LL+DQ     A   K    P+H A +K  ++IA  L+E   +  A     +  +HLA   GH ++V+LL   N N+ +
Sbjct:  551 PLHVAAKYGKLEVANLLLQKSASPDAAG-KSGLTPLHVAAHYDNQKVALLLLDQGASPHA-AAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNL 675          

HSP 7 Score: 62.003 bits (149), Expect = 1.337e-11
Identity = 30/128 (23.44%), Postives = 67/128 (52.34%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   ++I   L+    +  A   Q    ++H A ++GH+++V LL+ ++ ++  +  K    P+H A ++  + +A+VL+ +  +++A       P+H+ C  G+ +IV  L   +  +  +
Sbjct:  617 PLHIAAKKNQMDIATTLLEYGADANAVTRQGIA-SVHLAAQEGHVDMVSLLLGRNANVN-LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAK 742          

HSP 8 Score: 60.077 bits (144), Expect = 5.438e-11
Identity = 33/127 (25.98%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            PIH+A   GH+ IV  L++   +      +  E A+H A   G  E+V+ L+     +EA   K    P+H +   G  +I + L+++  +  A   +   P+HL+   GH ++   L D   ++ +
Sbjct:  419 PIHVAAFMGHVNIVSQLMHHGASPNTTNVR-GETALHMAARSGQAEVVRYLVQDGAQVEA-KAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSI 543          

HSP 9 Score: 60.077 bits (144), Expect = 5.763e-11
Identity = 37/159 (23.27%), Postives = 74/159 (46.54%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLI-------------------------------DQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L++   + ++   ++    +  A +QGH ++V LL+                               D + D+E+  G     P+H A   G++ +A +L+ +   ++    N++ P+H+A  RG+A +V LL D    I+ +
Sbjct:  126 PLYMAAQENHLEVVKFLLDNGAS-QSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSG---FTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK 280          

HSP 10 Score: 59.6918 bits (143), Expect = 7.483e-11
Identity = 34/127 (26.77%), Postives = 62/127 (48.82%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH ++ K+L++KK N  A +  N    +H AC++  +++++LL+     I+A+       PIH A   GH+ I   L+    +     +     +H+A   G AE+V  L      +E +
Sbjct:  354 LHVAAHCGHYKVAKVLLDKKANPNA-KALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLT-PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAK 478          

HSP 11 Score: 52.7582 bits (125), Expect = 2.353e-8
Identity = 28/118 (23.73%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            +H+A ++G  E+V+ L+     +EA + ++ +  +H +   G  +IV+ L+ Q     A        P+H +  +GH ++A  L++   ++         P+H+A   G  E+  LL 
Sbjct:  453 LHMAARSGQAEVVRYLVQDGAQVEA-KAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 568          

HSP 12 Score: 50.447 bits (119), Expect = 1.458e-7
Identity = 30/120 (25.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA +   + + ++L+N+  +++A + +     +H  C  G+++IV  L+     + A   K KN   P+H A ++GH  I  VL++   +     +N    + +A   G+  +V  L
Sbjct:  683 PLHLAAQEDRVNVAEVLVNQGAHVDA-QTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNA---KTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTL 798          

HSP 13 Score: 49.6766 bits (117), Expect = 2.478e-7
Identity = 26/128 (20.31%), Postives = 63/128 (49.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A    + ++  LL+++  +  A   +N    +H A ++  ++I   L++   D  A+  +     +H A ++GH+++  +L+ +  N+     + L P+HLA       +  +L +   +++ Q
Sbjct:  584 PLHVAAHYDNQKVALLLLDQGASPHAAA-KNGYTPLHIAAKKNQMDIATTLLEYGADANAVT-RQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQ 709          

HSP 14 Score: 48.9062 bits (115), Expect = 5.465e-7
Identity = 26/119 (21.85%), Postives = 59/119 (49.58%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +H+A +    +   LL+    N +    ++    +H A   G++ +  LL+++   ++    +    P+H A ++G+  + K+L+++   I+A   + L P+H     GH ++V +L D
Sbjct:  189 LHIAARKDDTKAAALLLQNDNNADV-ESKSGFTPLHIAAHYGNINVATLLLNRAAAVD-FTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 305          
BLAST of SMED30009057 vs. Ensembl Celegans
Match: trp-4 (TRP (Transient receptor potential) channel family [Source:UniProtKB/TrEMBL;Acc:Q9GRV5])

HSP 1 Score: 77.7962 bits (190), Expect = 1.403e-17
Identity = 37/125 (29.60%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+HLA ++GH  +V++L+N+   ++A     +   +H A +QGH+ +V +L+ +    +     W+ R P+H A + GH E+  +LI +  NI     N    +H A   GH  +V L  D++ +
Sbjct: 1208 PLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRSTQ-QQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSAD 1331          

HSP 2 Score: 61.6178 bits (148), Expect = 6.133e-12
Identity = 34/120 (28.33%), Postives = 56/120 (46.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG-GKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH+ +V +L+++    +  +       +H A + GH E+V LLI Q  +I  +    W    +HFA   GHL + K+ I+   +  A       P+  A    H E +  L 
Sbjct: 1242 PLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTG--LHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLL 1359          

HSP 3 Score: 60.8474 bits (146), Expect = 1.356e-11
Identity = 37/124 (29.84%), Postives = 61/124 (49.19%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQH-CDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            +HLA  NGHL +V LL+  K  + + + +  E  +H A + GH+++V +L+  H   +EAI        +HFA + G L +++ L+    N  A       P+HLA      ++V L      N
Sbjct:  924 LHLAAFNGHLSLVHLLLQHKAFVNS-KSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITLD-NQTALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNN 1045          

HSP 4 Score: 57.7658 bits (138), Expect = 1.692e-10
Identity = 28/93 (30.11%), Postives = 49/93 (52.69%), Query Frame = 3
Query:  117 IHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIE-AWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            +H A + GH  +V++L++Q   ++A        P+H A ++GH+ +  +L+ +    + A       P+HLA   GH E+V+LL     NI V
Sbjct: 1209 LHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINV 1301          

HSP 5 Score: 56.9954 bits (136), Expect = 2.590e-10
Identity = 34/123 (27.64%), Postives = 60/123 (48.78%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQ-NSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            S PIHLA K G++ IV+LL++   + +  +   N +  +H A   G++E V+  I   CD   +  +    P+H   E G   + K++ + + +       +  P+H+A  RG   +V  L D
Sbjct:  458 SLPIHLAFKFGNVNIVELLLSGPSDEQTRKADGNGDTLLHLAARSGNIEAVRTAIAAGCDNANVQNRVGRTPLHEVAEVGDQNMLKIMFKLRADANIHDKEDKTPVHVAAERGDTSMVESLID 580          

HSP 6 Score: 49.6766 bits (117), Expect = 1.012e-7
Identity = 33/120 (27.50%), Postives = 55/120 (45.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCN---IEACRFQNSERAIHFACEQGHLEIVK--LLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIV 356
            P+HLA +N   ++VKL +  + N   +      N     H A  +G L +V+  ++ID+   I+A     +   +H A   GH  I K+L+E   N E    + +  +HL    G   I+
Sbjct: 1023 PLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANAEDENSHGMTALHLGAKNGFISIL 1142          

HSP 7 Score: 49.6766 bits (117), Expect = 1.072e-7
Identity = 28/122 (22.95%), Postives = 59/122 (48.36%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCN-----IEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +H+A ++G+  ++  ++NK        ++  + +N    +  AC +GH  +  +L+  H  I+    +     +H A   GHL +  +L++ K  + +       P+HLA   GH ++V +L
Sbjct:  852 MHMAARSGNQAVLLAMVNKIGAGAVQIVQNKQSKNGWSPLLEACARGHSGVANILLKHHARIDVFD-EMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVL 972          

HSP 8 Score: 48.521 bits (114), Expect = 2.167e-7
Identity = 32/129 (24.81%), Postives = 63/129 (48.84%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLE---IAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            +H A   G  ++VK+LI +  N++  R +++  A+H A + G   +V+ L+    DI   GG+     +H A      E    A +L++     +   ++    +H+A   G+ +I+ LL D N + ++
Sbjct:  630 LHSAAAAGFNDVVKMLIARGTNVDV-RTRDNYTALHVAVQSGKASVVETLLGSGADIHVKGGELGQTALHIAASLNGAESRDCAMMLLKSGGQPDVAQMDGETCLHIAARSGNKDIMRLLLDENADSKI 757          

HSP 9 Score: 47.7506 bits (112), Expect = 5.260e-7
Identity = 32/120 (26.67%), Postives = 61/120 (50.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLI-NKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN----IEAWGINNLRPIHLACWRGHAEIV 356
            P+HLA ++GH+++V +L+ +    +EA    N + A+HFA + G L + + L+    +  A   K +  P+H A E    ++ K+ ++ + N    + A   N     H+A  +G   +V
Sbjct:  956 PLHLAAQHGHVKVVNVLVQDHGAALEAITLDN-QTALHFAAKFGQLAVSQTLLALGANPNARDDKGQT-PLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVV 1073          

HSP 10 Score: 43.8986 bits (102), Expect = 9.353e-6
Identity = 30/128 (23.44%), Postives = 57/128 (44.53%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A + G   +V+ LI+K       R ++    +H A   GH       + +   +  +  K     +H A   G  ++ K+LI +  N++    +N   +H+A   G A +V  L  +  +I V+
Sbjct:  562 PVHVAAERGDTSMVESLIDKFGGSIRARTRDGSTLLHIAACSGHTSTALAFLKRGVPL-FMPNKKGALGLHSAAAAGFNDVVKMLIARGTNVDVRTRDNYTALHVAVQSGKASVVETLLGSGADIHVK 688          
BLAST of SMED30009057 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 69.707 bits (169), Expect = 9.209e-15
Identity = 39/122 (31.97%), Postives = 64/122 (52.46%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +AC NGH +IV+LL+ +  NIE  R +     +  A   GH  +V++L+  H  IEA   + K+  +  AC  G  ++ ++L+    N E   +++  P+ LA   G+ EIV +L  A   I
Sbjct:   48 IACANGHKDIVELLLKEGANIEH-RDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEI 168          

HSP 2 Score: 59.3066 bits (142), Expect = 4.933e-11
Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            + LAC  G  ++V+LL+    N E  R  +    +  A   G++EIV +L+    +I +  G K    P+  A   GH E  +VL+EK  +I A    N    + LA ++G  E+V LL   N N+E
Sbjct:  113 LSLACSGGRKDVVELLLAHGANKEH-RNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVE 238          

HSP 3 Score: 51.9878 bits (123), Expect = 1.535e-8
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLR--PIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ LA  NGH E  ++L+ K  +I A    N   A+  A  QG  E+VKLL+  + ++E    K    P+      G++++  +LI    +  A  +   +   + ++  +GH + V +L + +  ++V+
Sbjct:  180 PLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVEH-RAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVDVR 308          

HSP 4 Score: 47.7506 bits (112), Expect = 4.532e-7
Identity = 29/122 (23.77%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            P+      G++++  LLI    +  A   Q + + A+  + E+GH + V++L++    ++ +  K     +  AC  G+L  A+ L+EK  + + +    + P+  A  +GH EIV  + ++
Sbjct:  247 PLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVD-VRNKKGCTALWLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 367          
BLAST of SMED30009057 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 69.707 bits (169), Expect = 9.217e-15
Identity = 39/122 (31.97%), Postives = 64/122 (52.46%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +AC NGH +IV+LL+ +  NIE  R +     +  A   GH  +V++L+  H  IEA   + K+  +  AC  G  ++ ++L+    N E   +++  P+ LA   G+ EIV +L  A   I
Sbjct:   48 IACANGHKDIVELLLKEGANIEH-RDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEI 168          

HSP 2 Score: 59.3066 bits (142), Expect = 4.937e-11
Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            + LAC  G  ++V+LL+    N E  R  +    +  A   G++EIV +L+    +I +  G K    P+  A   GH E  +VL+EK  +I A    N    + LA ++G  E+V LL   N N+E
Sbjct:  113 LSLACSGGRKDVVELLLAHGANKEH-RNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVE 238          

HSP 3 Score: 51.9878 bits (123), Expect = 1.536e-8
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLR--PIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ LA  NGH E  ++L+ K  +I A    N   A+  A  QG  E+VKLL+  + ++E    K    P+      G++++  +LI    +  A  +   +   + ++  +GH + V +L + +  ++V+
Sbjct:  180 PLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVEH-RAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVDVR 308          

HSP 4 Score: 47.7506 bits (112), Expect = 4.536e-7
Identity = 29/122 (23.77%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            P+      G++++  LLI    +  A   Q + + A+  + E+GH + V++L++    ++ +  K     +  AC  G+L  A+ L+EK  + + +    + P+  A  +GH EIV  + ++
Sbjct:  247 PLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVD-VRNKKGCTALWLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 367          
BLAST of SMED30009057 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 69.707 bits (169), Expect = 9.236e-15
Identity = 39/122 (31.97%), Postives = 64/122 (52.46%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +AC NGH +IV+LL+ +  NIE  R +     +  A   GH  +V++L+  H  IEA   + K+  +  AC  G  ++ ++L+    N E   +++  P+ LA   G+ EIV +L  A   I
Sbjct:  126 IACANGHKDIVELLLKEGANIEH-RDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEI 246          

HSP 2 Score: 59.3066 bits (142), Expect = 4.947e-11
Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            + LAC  G  ++V+LL+    N E  R  +    +  A   G++EIV +L+    +I +  G K    P+  A   GH E  +VL+EK  +I A    N    + LA ++G  E+V LL   N N+E
Sbjct:  191 LSLACSGGRKDVVELLLAHGANKEH-RNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVE 316          

HSP 3 Score: 51.9878 bits (123), Expect = 1.539e-8
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLR--PIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ LA  NGH E  ++L+ K  +I A    N   A+  A  QG  E+VKLL+  + ++E    K    P+      G++++  +LI    +  A  +   +   + ++  +GH + V +L + +  ++V+
Sbjct:  258 PLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVEH-RAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVDVR 386          

HSP 4 Score: 47.7506 bits (112), Expect = 4.545e-7
Identity = 29/122 (23.77%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            P+      G++++  LLI    +  A   Q + + A+  + E+GH + V++L++    ++ +  K     +  AC  G+L  A+ L+EK  + + +    + P+  A  +GH EIV  + ++
Sbjct:  325 PLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVD-VRNKKGCTALWLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 445          
BLAST of SMED30009057 vs. Ensembl Celegans
Match: mask-1 (Ankyrin repeat and KH domain-containing protein mask-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21920])

HSP 1 Score: 69.707 bits (169), Expect = 9.236e-15
Identity = 39/122 (31.97%), Postives = 64/122 (52.46%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +AC NGH +IV+LL+ +  NIE  R +     +  A   GH  +V++L+  H  IEA   + K+  +  AC  G  ++ ++L+    N E   +++  P+ LA   G+ EIV +L  A   I
Sbjct:  128 IACANGHKDIVELLLKEGANIEH-RDKKGFSPLIIAATAGHSSVVEVLLKNHAAIEAQSDRTKDTALSLACSGGRKDVVELLLAHGANKEHRNVSDYTPLSLASSGGYIEIVNMLLTAGSEI 248          

HSP 2 Score: 59.3066 bits (142), Expect = 4.947e-11
Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            + LAC  G  ++V+LL+    N E  R  +    +  A   G++EIV +L+    +I +  G K    P+  A   GH E  +VL+EK  +I A    N    + LA ++G  E+V LL   N N+E
Sbjct:  193 LSLACSGGRKDVVELLLAHGANKEH-RNVSDYTPLSLASSGGYIEIVNMLLTAGSEINSRTGSKLGISPLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVE 318          

HSP 3 Score: 51.9878 bits (123), Expect = 1.539e-8
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLR--PIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ LA  NGH E  ++L+ K  +I A    N   A+  A  QG  E+VKLL+  + ++E    K    P+      G++++  +LI    +  A  +   +   + ++  +GH + V +L + +  ++V+
Sbjct:  260 PLMLASMNGHREATRVLLEKGSDINAQIETNRNTALTLASFQGRTEVVKLLLAYNANVEH-RAKTGLTPLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVDVR 388          

HSP 4 Score: 47.7506 bits (112), Expect = 4.545e-7
Identity = 29/122 (23.77%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            P+      G++++  LLI    +  A   Q + + A+  + E+GH + V++L++    ++ +  K     +  AC  G+L  A+ L+EK  + + +    + P+  A  +GH EIV  + ++
Sbjct:  327 PLMECASGGYVDVGNLLIAAGADTNASPVQQTKDTALTISAEKGHEKFVRMLLNGDAAVD-VRNKKGCTALWLACNGGYLSTAQALLEKGADPDMFDNRKISPMMAAFRKGHVEIVKYMVNS 447          
BLAST of SMED30009057 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303118)

HSP 1 Score: 72.7886 bits (177), Expect = 1.071e-15
Identity = 34/120 (28.33%), Postives = 73/120 (60.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G+++IV LL+     ++A   ++   A+H A ++G  E+  +LI+    ++A   K    P+H   + GH+++A++L++K+ +++A G N + P+H+AC   + ++  LL +
Sbjct:  472 PLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDA-ATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 2 Score: 65.855 bits (159), Expect = 2.781e-13
Identity = 31/125 (24.80%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH ++V +L+ +   I A + +N    +H A +  H++  ++L+     ++ +   +    +H A   GH+ +AK+L+++  +  A  +N   P+H+AC +   ++V LL     +I
Sbjct:  274 PLHCAARSGHEQVVDMLLERGAPISA-KTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLT-ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 3 Score: 65.0846 bits (157), Expect = 4.972e-13
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HLA +    +I+++L+     ++A R +  +  +H A   G+++IV LL+     ++A   K     +H A ++G  E+A VLIE    ++A       P+HL    GH ++  LL     +++ Q
Sbjct:  439 PLHLAARANQTDIIRILLRNGAQVDA-RAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQ 564          

HSP 4 Score: 62.3882 bits (150), Expect = 4.305e-12
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI--EAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A +  H++  ++L+  +  ++     +   A+H A   GH+ + KLL+D++ D    A+ G     P+H AC+K  L++ ++L+    +I A   + L P+H+A + G   IV  L   + + +V
Sbjct:  307 PLHMAAQGEHVDAARILLYHRAPVDEVTV-DYLTALHVAAHCGHVRVAKLLLDRNADANARALNG---FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDV 431          

HSP 5 Score: 58.151 bits (139), Expect = 1.235e-10
Identity = 37/159 (23.27%), Postives = 70/159 (44.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG---------GKWKNR-----------------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  K GH+++ +LL+ K+ +++A + +N    +H AC   + ++  LL+++     A              KN+                       P+H + ++GH EI+ +LIE K  +     N L P+HL     +  +  +L     NI++
Sbjct:  538 PLHLTAKYGHIKVAQLLLQKEADVDA-QGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM 695          

HSP 6 Score: 57.3806 bits (137), Expect = 2.810e-10
Identity = 33/128 (25.78%), Postives = 59/128 (46.09%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HL+ + GH EI  LLI  K  +     +N    +H   ++ ++ + ++L     +I+ +  K    P+H A   G   + + L++   N++A       P+H    +GH  IV LL +   N   Q
Sbjct:  637 PLHLSSQEGHAEISNLLIEHKAAVNHPA-KNGLTPMHLCAQEDNVNVAEILEKNGANID-MATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQ 762          

HSP 7 Score: 55.8398 bits (133), Expect = 1.026e-9
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A K   ++   LL++   N +    ++    +H A   G+  I  LLI +  D+     K    P+H A + G   +  +L+EK  NIEA   + L P+H A   GH ++V +L +    I  +
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTS-KSGFTPLHIASHYGNQNIANLLIQKGADVN-YSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAK 300          

HSP 8 Score: 54.299 bits (129), Expect = 2.857e-9
Identity = 29/127 (22.83%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K+GH+ +V  L+ +   +++   +    A+H A   G  E+VKLL++ +  +  +  +    P++ A ++ H  + ++L+    N      +   P+ +A  +GH ++V +L +++   +V+
Sbjct:   48 LHLASKDGHIHVVSELLRRGAIVDSAT-KKGNTALHIASLAGQEEVVKLLLEHNASVN-VQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR 172          

HSP 9 Score: 53.9138 bits (128), Expect = 4.908e-9
Identity = 34/128 (26.56%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ +A + GH ++V +L+         R +    A+H A ++  ++   LL+D   + + +  K    P+H A   G+  IA +LI+K  ++     +N+ P+H+A   G   +V+LL +   NIE +
Sbjct:  146 PLAVAMQQGHDKVVAVLLESDT-----RGKVRLPALHIAAKKDDVKAATLLLDNDHNPD-VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAK 267          

HSP 10 Score: 51.9878 bits (123), Expect = 2.069e-8
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH+ + KLL+++  +  A R  N    +H AC++  L++V+LL+     I A        P+H A   G + I   L++   + +   +    P+HLA      +I+ +L      ++ +
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANA-RALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLT-PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDAR 465          

HSP 11 Score: 49.2914 bits (116), Expect = 1.662e-7
Identity = 33/121 (27.27%), Postives = 58/121 (47.93%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEA-CRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+LE  ++L + K NI+      N   A+H A + GH+ +V  L+ +   +++   K  N  +H A   G  E+ K+L+E   ++     N   P+++A    H  +V LL     N
Sbjct:   18 AARAGNLE--RVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDS-ATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGAN 135          

HSP 12 Score: 48.9062 bits (115), Expect = 2.615e-7
Identity = 28/118 (23.73%), Postives = 55/118 (46.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  +  ++ + ++L     NI+    +     +H A   G   +V+ L+    +++A        P+H   ++GH  I  +L+E K N  A  +N   P+H+A   G+  ++  L
Sbjct:  670 PMHLCAQEDNVNVAEILEKNGANIDMAT-KAGYTPLHVASHFGQANMVRFLLQNGANVDA-ATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30009057 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303122)

HSP 1 Score: 72.7886 bits (177), Expect = 1.073e-15
Identity = 34/120 (28.33%), Postives = 73/120 (60.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G+++IV LL+     ++A   ++   A+H A ++G  E+  +LI+    ++A   K    P+H   + GH+++A++L++K+ +++A G N + P+H+AC   + ++  LL +
Sbjct:  472 PLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDA-ATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 2 Score: 65.855 bits (159), Expect = 2.786e-13
Identity = 31/125 (24.80%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH ++V +L+ +   I A + +N    +H A +  H++  ++L+     ++ +   +    +H A   GH+ +AK+L+++  +  A  +N   P+H+AC +   ++V LL     +I
Sbjct:  274 PLHCAARSGHEQVVDMLLERGAPISA-KTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLT-ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 3 Score: 65.0846 bits (157), Expect = 4.980e-13
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HLA +    +I+++L+     ++A R +  +  +H A   G+++IV LL+     ++A   K     +H A ++G  E+A VLIE    ++A       P+HL    GH ++  LL     +++ Q
Sbjct:  439 PLHLAARANQTDIIRILLRNGAQVDA-RAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQ 564          

HSP 4 Score: 62.3882 bits (150), Expect = 4.312e-12
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI--EAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A +  H++  ++L+  +  ++     +   A+H A   GH+ + KLL+D++ D    A+ G     P+H AC+K  L++ ++L+    +I A   + L P+H+A + G   IV  L   + + +V
Sbjct:  307 PLHMAAQGEHVDAARILLYHRAPVDEVTV-DYLTALHVAAHCGHVRVAKLLLDRNADANARALNG---FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDV 431          

HSP 5 Score: 58.151 bits (139), Expect = 1.237e-10
Identity = 37/159 (23.27%), Postives = 70/159 (44.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG---------GKWKNR-----------------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  K GH+++ +LL+ K+ +++A + +N    +H AC   + ++  LL+++     A              KN+                       P+H + ++GH EI+ +LIE K  +     N L P+HL     +  +  +L     NI++
Sbjct:  538 PLHLTAKYGHIKVAQLLLQKEADVDA-QGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM 695          

HSP 6 Score: 57.3806 bits (137), Expect = 2.814e-10
Identity = 33/128 (25.78%), Postives = 59/128 (46.09%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HL+ + GH EI  LLI  K  +     +N    +H   ++ ++ + ++L     +I+ +  K    P+H A   G   + + L++   N++A       P+H    +GH  IV LL +   N   Q
Sbjct:  637 PLHLSSQEGHAEISNLLIEHKAAVNHPA-KNGLTPMHLCAQEDNVNVAEILEKNGANID-MATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQ 762          

HSP 7 Score: 55.8398 bits (133), Expect = 1.028e-9
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A K   ++   LL++   N +    ++    +H A   G+  I  LLI +  D+     K    P+H A + G   +  +L+EK  NIEA   + L P+H A   GH ++V +L +    I  +
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTS-KSGFTPLHIASHYGNQNIANLLIQKGADVN-YSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAK 300          

HSP 8 Score: 54.299 bits (129), Expect = 2.862e-9
Identity = 29/127 (22.83%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K+GH+ +V  L+ +   +++   +    A+H A   G  E+VKLL++ +  +  +  +    P++ A ++ H  + ++L+    N      +   P+ +A  +GH ++V +L +++   +V+
Sbjct:   48 LHLASKDGHIHVVSELLRRGAIVDSAT-KKGNTALHIASLAGQEEVVKLLLEHNASVN-VQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR 172          

HSP 9 Score: 53.9138 bits (128), Expect = 4.916e-9
Identity = 34/128 (26.56%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ +A + GH ++V +L+         R +    A+H A ++  ++   LL+D   + + +  K    P+H A   G+  IA +LI+K  ++     +N+ P+H+A   G   +V+LL +   NIE +
Sbjct:  146 PLAVAMQQGHDKVVAVLLESDT-----RGKVRLPALHIAAKKDDVKAATLLLDNDHNPD-VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAK 267          

HSP 10 Score: 51.9878 bits (123), Expect = 2.072e-8
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH+ + KLL+++  +  A R  N    +H AC++  L++V+LL+     I A        P+H A   G + I   L++   + +   +    P+HLA      +I+ +L      ++ +
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANA-RALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLT-PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDAR 465          

HSP 11 Score: 49.2914 bits (116), Expect = 1.665e-7
Identity = 33/121 (27.27%), Postives = 58/121 (47.93%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEA-CRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+LE  ++L + K NI+      N   A+H A + GH+ +V  L+ +   +++   K  N  +H A   G  E+ K+L+E   ++     N   P+++A    H  +V LL     N
Sbjct:   18 AARAGNLE--RVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDS-ATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGAN 135          

HSP 12 Score: 48.9062 bits (115), Expect = 2.619e-7
Identity = 28/118 (23.73%), Postives = 55/118 (46.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  +  ++ + ++L     NI+    +     +H A   G   +V+ L+    +++A        P+H   ++GH  I  +L+E K N  A  +N   P+H+A   G+  ++  L
Sbjct:  670 PMHLCAQEDNVNVAEILEKNGANIDMAT-KAGYTPLHVASHFGQANMVRFLLQNGANVDA-ATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30009057 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0344222)

HSP 1 Score: 72.7886 bits (177), Expect = 1.073e-15
Identity = 34/120 (28.33%), Postives = 73/120 (60.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G+++IV LL+     ++A   ++   A+H A ++G  E+  +LI+    ++A   K    P+H   + GH+++A++L++K+ +++A G N + P+H+AC   + ++  LL +
Sbjct:  472 PLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDA-ATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 2 Score: 65.855 bits (159), Expect = 2.787e-13
Identity = 31/125 (24.80%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH ++V +L+ +   I A + +N    +H A +  H++  ++L+     ++ +   +    +H A   GH+ +AK+L+++  +  A  +N   P+H+AC +   ++V LL     +I
Sbjct:  274 PLHCAARSGHEQVVDMLLERGAPISA-KTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLT-ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 3 Score: 65.0846 bits (157), Expect = 4.982e-13
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HLA +    +I+++L+     ++A R +  +  +H A   G+++IV LL+     ++A   K     +H A ++G  E+A VLIE    ++A       P+HL    GH ++  LL     +++ Q
Sbjct:  439 PLHLAARANQTDIIRILLRNGAQVDA-RAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQ 564          

HSP 4 Score: 62.3882 bits (150), Expect = 4.313e-12
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI--EAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A +  H++  ++L+  +  ++     +   A+H A   GH+ + KLL+D++ D    A+ G     P+H AC+K  L++ ++L+    +I A   + L P+H+A + G   IV  L   + + +V
Sbjct:  307 PLHMAAQGEHVDAARILLYHRAPVDEVTV-DYLTALHVAAHCGHVRVAKLLLDRNADANARALNG---FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDV 431          

HSP 5 Score: 58.151 bits (139), Expect = 1.238e-10
Identity = 37/159 (23.27%), Postives = 70/159 (44.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG---------GKWKNR-----------------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  K GH+++ +LL+ K+ +++A + +N    +H AC   + ++  LL+++     A              KN+                       P+H + ++GH EI+ +LIE K  +     N L P+HL     +  +  +L     NI++
Sbjct:  538 PLHLTAKYGHIKVAQLLLQKEADVDA-QGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM 695          

HSP 6 Score: 57.3806 bits (137), Expect = 2.815e-10
Identity = 33/128 (25.78%), Postives = 59/128 (46.09%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HL+ + GH EI  LLI  K  +     +N    +H   ++ ++ + ++L     +I+ +  K    P+H A   G   + + L++   N++A       P+H    +GH  IV LL +   N   Q
Sbjct:  637 PLHLSSQEGHAEISNLLIEHKAAVNHPA-KNGLTPMHLCAQEDNVNVAEILEKNGANID-MATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQ 762          

HSP 7 Score: 55.8398 bits (133), Expect = 1.028e-9
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A K   ++   LL++   N +    ++    +H A   G+  I  LLI +  D+     K    P+H A + G   +  +L+EK  NIEA   + L P+H A   GH ++V +L +    I  +
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTS-KSGFTPLHIASHYGNQNIANLLIQKGADVN-YSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAK 300          

HSP 8 Score: 54.299 bits (129), Expect = 2.863e-9
Identity = 29/127 (22.83%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K+GH+ +V  L+ +   +++   +    A+H A   G  E+VKLL++ +  +  +  +    P++ A ++ H  + ++L+    N      +   P+ +A  +GH ++V +L +++   +V+
Sbjct:   48 LHLASKDGHIHVVSELLRRGAIVDSAT-KKGNTALHIASLAGQEEVVKLLLEHNASVN-VQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR 172          

HSP 9 Score: 53.9138 bits (128), Expect = 4.917e-9
Identity = 34/128 (26.56%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ +A + GH ++V +L+         R +    A+H A ++  ++   LL+D   + + +  K    P+H A   G+  IA +LI+K  ++     +N+ P+H+A   G   +V+LL +   NIE +
Sbjct:  146 PLAVAMQQGHDKVVAVLLESDT-----RGKVRLPALHIAAKKDDVKAATLLLDNDHNPD-VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAK 267          

HSP 10 Score: 51.9878 bits (123), Expect = 2.073e-8
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH+ + KLL+++  +  A R  N    +H AC++  L++V+LL+     I A        P+H A   G + I   L++   + +   +    P+HLA      +I+ +L      ++ +
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANA-RALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLT-PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDAR 465          

HSP 11 Score: 49.2914 bits (116), Expect = 1.666e-7
Identity = 33/121 (27.27%), Postives = 58/121 (47.93%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEA-CRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+LE  ++L + K NI+      N   A+H A + GH+ +V  L+ +   +++   K  N  +H A   G  E+ K+L+E   ++     N   P+++A    H  +V LL     N
Sbjct:   18 AARAGNLE--RVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDS-ATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGAN 135          

HSP 12 Score: 48.9062 bits (115), Expect = 2.620e-7
Identity = 28/118 (23.73%), Postives = 55/118 (46.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  +  ++ + ++L     NI+    +     +H A   G   +V+ L+    +++A        P+H   ++GH  I  +L+E K N  A  +N   P+H+A   G+  ++  L
Sbjct:  670 PMHLCAQEDNVNVAEILEKNGANIDMAT-KAGYTPLHVASHFGQANMVRFLLQNGANVDA-ATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30009057 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303117)

HSP 1 Score: 72.7886 bits (177), Expect = 1.085e-15
Identity = 34/120 (28.33%), Postives = 73/120 (60.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G+++IV LL+     ++A   ++   A+H A ++G  E+  +LI+    ++A   K    P+H   + GH+++A++L++K+ +++A G N + P+H+AC   + ++  LL +
Sbjct:  472 PLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDA-ATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 2 Score: 65.855 bits (159), Expect = 2.817e-13
Identity = 31/125 (24.80%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH ++V +L+ +   I A + +N    +H A +  H++  ++L+     ++ +   +    +H A   GH+ +AK+L+++  +  A  +N   P+H+AC +   ++V LL     +I
Sbjct:  274 PLHCAARSGHEQVVDMLLERGAPISA-KTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLT-ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 3 Score: 65.0846 bits (157), Expect = 5.036e-13
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HLA +    +I+++L+     ++A R +  +  +H A   G+++IV LL+     ++A   K     +H A ++G  E+A VLIE    ++A       P+HL    GH ++  LL     +++ Q
Sbjct:  439 PLHLAARANQTDIIRILLRNGAQVDA-RAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQ 564          

HSP 4 Score: 62.3882 bits (150), Expect = 4.360e-12
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI--EAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A +  H++  ++L+  +  ++     +   A+H A   GH+ + KLL+D++ D    A+ G     P+H AC+K  L++ ++L+    +I A   + L P+H+A + G   IV  L   + + +V
Sbjct:  307 PLHMAAQGEHVDAARILLYHRAPVDEVTV-DYLTALHVAAHCGHVRVAKLLLDRNADANARALNG---FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDV 431          

HSP 5 Score: 58.151 bits (139), Expect = 1.251e-10
Identity = 37/159 (23.27%), Postives = 70/159 (44.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG---------GKWKNR-----------------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  K GH+++ +LL+ K+ +++A + +N    +H AC   + ++  LL+++     A              KN+                       P+H + ++GH EI+ +LIE K  +     N L P+HL     +  +  +L     NI++
Sbjct:  538 PLHLTAKYGHIKVAQLLLQKEADVDA-QGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM 695          

HSP 6 Score: 57.3806 bits (137), Expect = 2.846e-10
Identity = 33/128 (25.78%), Postives = 59/128 (46.09%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HL+ + GH EI  LLI  K  +     +N    +H   ++ ++ + ++L     +I+ +  K    P+H A   G   + + L++   N++A       P+H    +GH  IV LL +   N   Q
Sbjct:  637 PLHLSSQEGHAEISNLLIEHKAAVNHPA-KNGLTPMHLCAQEDNVNVAEILEKNGANID-MATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQ 762          

HSP 7 Score: 55.8398 bits (133), Expect = 1.039e-9
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A K   ++   LL++   N +    ++    +H A   G+  I  LLI +  D+     K    P+H A + G   +  +L+EK  NIEA   + L P+H A   GH ++V +L +    I  +
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTS-KSGFTPLHIASHYGNQNIANLLIQKGADVN-YSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAK 300          

HSP 8 Score: 54.299 bits (129), Expect = 2.893e-9
Identity = 29/127 (22.83%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K+GH+ +V  L+ +   +++   +    A+H A   G  E+VKLL++ +  +  +  +    P++ A ++ H  + ++L+    N      +   P+ +A  +GH ++V +L +++   +V+
Sbjct:   48 LHLASKDGHIHVVSELLRRGAIVDSAT-KKGNTALHIASLAGQEEVVKLLLEHNASVN-VQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR 172          

HSP 9 Score: 53.9138 bits (128), Expect = 4.970e-9
Identity = 34/128 (26.56%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ +A + GH ++V +L+         R +    A+H A ++  ++   LL+D   + + +  K    P+H A   G+  IA +LI+K  ++     +N+ P+H+A   G   +V+LL +   NIE +
Sbjct:  146 PLAVAMQQGHDKVVAVLLESDT-----RGKVRLPALHIAAKKDDVKAATLLLDNDHNPD-VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAK 267          

HSP 10 Score: 51.9878 bits (123), Expect = 2.095e-8
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH+ + KLL+++  +  A R  N    +H AC++  L++V+LL+     I A        P+H A   G + I   L++   + +   +    P+HLA      +I+ +L      ++ +
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANA-RALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLT-PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDAR 465          

HSP 11 Score: 49.2914 bits (116), Expect = 1.683e-7
Identity = 33/121 (27.27%), Postives = 58/121 (47.93%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEA-CRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+LE  ++L + K NI+      N   A+H A + GH+ +V  L+ +   +++   K  N  +H A   G  E+ K+L+E   ++     N   P+++A    H  +V LL     N
Sbjct:   18 AARAGNLE--RVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDS-ATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGAN 135          

HSP 12 Score: 48.9062 bits (115), Expect = 2.648e-7
Identity = 28/118 (23.73%), Postives = 55/118 (46.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  +  ++ + ++L     NI+    +     +H A   G   +V+ L+    +++A        P+H   ++GH  I  +L+E K N  A  +N   P+H+A   G+  ++  L
Sbjct:  670 PMHLCAQEDNVNVAEILEKNGANIDMAT-KAGYTPLHVASHFGQANMVRFLLQNGANVDA-ATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30009057 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303121)

HSP 1 Score: 72.7886 bits (177), Expect = 1.085e-15
Identity = 34/120 (28.33%), Postives = 73/120 (60.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G+++IV LL+     ++A   ++   A+H A ++G  E+  +LI+    ++A   K    P+H   + GH+++A++L++K+ +++A G N + P+H+AC   + ++  LL +
Sbjct:  472 PLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDA-ATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 2 Score: 65.855 bits (159), Expect = 2.817e-13
Identity = 31/125 (24.80%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH ++V +L+ +   I A + +N    +H A +  H++  ++L+     ++ +   +    +H A   GH+ +AK+L+++  +  A  +N   P+H+AC +   ++V LL     +I
Sbjct:  274 PLHCAARSGHEQVVDMLLERGAPISA-KTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLT-ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 3 Score: 65.0846 bits (157), Expect = 5.037e-13
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HLA +    +I+++L+     ++A R +  +  +H A   G+++IV LL+     ++A   K     +H A ++G  E+A VLIE    ++A       P+HL    GH ++  LL     +++ Q
Sbjct:  439 PLHLAARANQTDIIRILLRNGAQVDA-RAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT-KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQ 564          

HSP 4 Score: 62.3882 bits (150), Expect = 4.360e-12
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI--EAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A +  H++  ++L+  +  ++     +   A+H A   GH+ + KLL+D++ D    A+ G     P+H AC+K  L++ ++L+    +I A   + L P+H+A + G   IV  L   + + +V
Sbjct:  307 PLHMAAQGEHVDAARILLYHRAPVDEVTV-DYLTALHVAAHCGHVRVAKLLLDRNADANARALNG---FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDV 431          

HSP 5 Score: 58.151 bits (139), Expect = 1.251e-10
Identity = 37/159 (23.27%), Postives = 70/159 (44.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG---------GKWKNR-----------------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  K GH+++ +LL+ K+ +++A + +N    +H AC   + ++  LL+++     A              KN+                       P+H + ++GH EI+ +LIE K  +     N L P+HL     +  +  +L     NI++
Sbjct:  538 PLHLTAKYGHIKVAQLLLQKEADVDA-QGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM 695          

HSP 6 Score: 57.3806 bits (137), Expect = 2.846e-10
Identity = 33/128 (25.78%), Postives = 59/128 (46.09%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+HL+ + GH EI  LLI  K  +     +N    +H   ++ ++ + ++L     +I+ +  K    P+H A   G   + + L++   N++A       P+H    +GH  IV LL +   N   Q
Sbjct:  637 PLHLSSQEGHAEISNLLIEHKAAVNHPA-KNGLTPMHLCAQEDNVNVAEILEKNGANID-MATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQ 762          

HSP 7 Score: 55.8398 bits (133), Expect = 1.039e-9
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A K   ++   LL++   N +    ++    +H A   G+  I  LLI +  D+     K    P+H A + G   +  +L+EK  NIEA   + L P+H A   GH ++V +L +    I  +
Sbjct:  176 LHIAAKKDDVKAATLLLDNDHNPDVTS-KSGFTPLHIASHYGNQNIANLLIQKGADVN-YSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAK 300          

HSP 8 Score: 54.299 bits (129), Expect = 2.894e-9
Identity = 29/127 (22.83%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K+GH+ +V  L+ +   +++   +    A+H A   G  E+VKLL++ +  +  +  +    P++ A ++ H  + ++L+    N      +   P+ +A  +GH ++V +L +++   +V+
Sbjct:   48 LHLASKDGHIHVVSELLRRGAIVDSAT-KKGNTALHIASLAGQEEVVKLLLEHNASVN-VQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR 172          

HSP 9 Score: 53.9138 bits (128), Expect = 4.970e-9
Identity = 34/128 (26.56%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ +A + GH ++V +L+         R +    A+H A ++  ++   LL+D   + + +  K    P+H A   G+  IA +LI+K  ++     +N+ P+H+A   G   +V+LL +   NIE +
Sbjct:  146 PLAVAMQQGHDKVVAVLLESDT-----RGKVRLPALHIAAKKDDVKAATLLLDNDHNPD-VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAK 267          

HSP 10 Score: 51.9878 bits (123), Expect = 2.095e-8
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A   GH+ + KLL+++  +  A R  N    +H AC++  L++V+LL+     I A        P+H A   G + I   L++   + +   +    P+HLA      +I+ +L      ++ +
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANA-RALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLT-PLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDAR 465          

HSP 11 Score: 49.2914 bits (116), Expect = 1.683e-7
Identity = 33/121 (27.27%), Postives = 58/121 (47.93%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEA-CRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+LE  ++L + K NI+      N   A+H A + GH+ +V  L+ +   +++   K  N  +H A   G  E+ K+L+E   ++     N   P+++A    H  +V LL     N
Sbjct:   18 AARAGNLE--RVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDS-ATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGAN 135          

HSP 12 Score: 48.9062 bits (115), Expect = 2.648e-7
Identity = 28/118 (23.73%), Postives = 55/118 (46.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  +  ++ + ++L     NI+    +     +H A   G   +V+ L+    +++A        P+H   ++GH  I  +L+E K N  A  +N   P+H+A   G+  ++  L
Sbjct:  670 PMHLCAQEDNVNVAEILEKNGANIDMAT-KAGYTPLHVASHFGQANMVRFLLQNGANVDA-ATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30009057 vs. Ensembl Zebrafish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 77.7962 bits (190), Expect = 2.277e-17
Identity = 38/126 (30.16%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I+++L+++   I+A + +N    IH A +  HL+ ++ L+  + +I+ I       P+H A   GH  +AKVL++K        +N   P+H+AC + H  ++ LL   + ++E
Sbjct:  275 PLHCAARNGHVRIIEILLDQGAPIQA-KTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLE 398          

HSP 2 Score: 73.1738 bits (178), Expect = 1.120e-15
Identity = 39/125 (31.20%), Postives = 63/125 (50.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H++   GHL IVK+L+ K  +  A   +  E  +H A   GH E+ + L+     ++A   K    P+H A   GH E+ K+L+E K N  +       P+H+A   GH +  ++L D N  +
Sbjct:  407 PLHVSSFMGHLNIVKILMQKGASPNASNVK-VETPLHMASRAGHCEVAEFLLQNAAPVDA-KAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQL 529          

HSP 3 Score: 69.707 bits (169), Expect = 1.546e-14
Identity = 35/119 (29.41%), Postives = 66/119 (55.46%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+     ++    +H   ++GH+ I  +L+     + A   +    P+H AC  G++++ K L++++ N+      +  P+H A  +GH +IVTLL 
Sbjct:  638 PLHLASQEGQPDMVLLLISKQANVNLGN-KSGLTPLHLVAQEGHVGIADILVKHGASVYA-ATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLL 754          

HSP 4 Score: 65.0846 bits (157), Expect = 7.509e-13
Identity = 31/124 (25.00%), Postives = 62/124 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   +  +      + P+H A  +GH + A +L++    +         P+H+A   G  ++  LL +   N
Sbjct:  440 PLHMASRAGHCEVAEFLLQNAAPVDA-KAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTA-GHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGAN 561          

HSP 5 Score: 62.003 bits (149), Expect = 7.372e-12
Identity = 33/128 (25.78%), Postives = 63/128 (49.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K    E+   L+    +  A   Q     +H A ++G  ++V LLI +  ++  +G K    P+H   ++GH+ IA +L++   ++ A       P+H+AC  G+ ++V  L     N+  +
Sbjct:  605 PLHIASKQNQAEVASSLLQHGASANAESLQGVT-PLHLASQEGQPDMVLLLISKQANVN-LGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCK 730          

HSP 6 Score: 53.5286 bits (127), Expect = 6.728e-9
Identity = 31/127 (24.41%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G +++  LL+ +  N  A   +     +H A    +L++V LL+ +     +   +    P+H A ++   E+A  L++   +  A  +  + P+HLA   G  ++V LL     N+ +
Sbjct:  539 PLHVAAKYGKVDVAVLLLERGANPNAAG-KVGLTPLHVAVHHNNLDVVNLLLSKGGSPHS-AARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNL 663          

HSP 7 Score: 52.373 bits (124), Expect = 1.717e-8
Identity = 32/119 (26.89%), Postives = 60/119 (50.42%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++    +E    +    A+H A   G  ++V  L++   ++ A   K    P++ A ++ HLE+ K L++   N      +   P+ +A  +GH  +V LL +
Sbjct:   49 LHLASKEGHVKMVLELLHHGIVLETTT-KKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLIN 165          

HSP 8 Score: 51.2174 bits (121), Expect = 4.213e-8
Identity = 36/156 (23.08%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ----HCDIEAIGGKWKN------------------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L++   N +    ++    +  A +QGH  +V LLI+        + A+    +N                         P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    I+ +
Sbjct:  114 PLYMAAQENHLEVVKFLLDNGAN-QTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAK 268          

HSP 9 Score: 50.8322 bits (120), Expect = 6.695e-8
Identity = 29/127 (22.83%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++  D+     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  I+ +L D    I+ +
Sbjct:  177 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLNVAQLLLNRGADVN-FTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAK 301          

HSP 10 Score: 49.2914 bits (116), Expect = 2.369e-7
Identity = 27/118 (22.88%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I  +L+    ++ A   +     +H AC  G++++VK L+ Q  ++     +    P+H A ++GH +I  +L++          N    + +A   G+  ++ +L
Sbjct:  671 PLHLVAQEGHVGIADILVKHGASVYAAT-RMGYTPLHVACHYGNIKMVKFLLQQQANVNC-KTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVL 786          

HSP 11 Score: 47.3654 bits (111), Expect = 1.015e-6
Identity = 31/120 (25.83%), Postives = 52/120 (43.33%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A ++G+LE     I    +I     QN    +H A ++GH+++V  L+     +E    K  N  +H A   G  ++   L+    N+ A       P+++A    H E+V  L D   N
Sbjct:   19 AARSGNLEKALDHIKNGIDINTAN-QNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKK-GNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGAN 136          
BLAST of SMED30009057 vs. Ensembl Zebrafish
Match: ankdd1a (ankyrin repeat and death domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-070615-8])

HSP 1 Score: 76.2554 bits (186), Expect = 6.722e-17
Identity = 41/125 (32.80%), Postives = 71/125 (56.80%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSER-AIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H A + GH+ I++ ++    N++  + +NS + A H A E GHLE+V+ LI   C    +  K  N  +H A ++GH ++ + ++E   NI+   I+ +  +HLA   GH E + LL +A CN+
Sbjct:  118 LHCAAQRGHITILEYIMEDLENVQLNKVENSGKTAFHLAAEHGHLEVVEFLIGMGC-AHNLKDKHGNTALHLAAKQGHSDVLQKIMETGENIDERNIDGMTALHLASEGGHYECIRLLLEAGCNV 241          

HSP 2 Score: 61.2326 bits (147), Expect = 1.573e-11
Identity = 38/128 (29.69%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
              HLA ++GHLE+V+ LI   C     + ++   A+H A +QGH ++++ +++  ++ D   I G      +H A E GH E  ++L+E  CN+     +    +HL      A  V LL  A  N++
Sbjct:  152 AFHLAAEHGHLEVVEFLIGMGC-AHNLKDKHGNTALHLAAKQGHSDVLQKIMETGENIDERNIDGM---TALHLASEGGHYECIRLLLEAGCNVNELTDSKRTALHLVAQHASASEVRLLIQAGINLD 275          

HSP 3 Score: 60.8474 bits (146), Expect = 1.651e-11
Identity = 33/127 (25.98%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
             +HLA K GH ++++ ++    NI+  R  +   A+H A E GH E ++LL++  C++  +    K   +H   +       ++LI+   N+++    ++  +HLA      EIV  L +A C++++
Sbjct:  185 ALHLAAKQGHSDVLQKIMETGENIDE-RNIDGMTALHLASEGGHYECIRLLLEAGCNVNELTDS-KRTALHLVAQHASASEVRLLIQAGINLDSVDTQHVSALHLAVLNNSTEIVKDLIEAGCDLDI 309          

HSP 4 Score: 48.1358 bits (113), Expect = 5.411e-7
Identity = 32/128 (25.00%), Postives = 61/128 (47.66%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSER-AIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
             +HLA + GH E ++LL+   CN+      +S+R A+H   +      V+LLI    +++++  +  +  +H A      EI K LIE  C+++ +       +H+A   G   I   +  +  N+ +
Sbjct:  218 ALHLASEGGHYECIRLLLEAGCNVN--ELTDSKRTALHLVAQHASASEVRLLIQAGINLDSVDTQHVS-ALHLAVLNNSTEIVKDLIEAGCDLDIFDNRLQTALHIAAEHGRLNIAETILISGVNLNL 342          
BLAST of SMED30009057 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 75.485 bits (184), Expect = 1.585e-16
Identity = 40/124 (32.26%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+  + LL+    ++EA   ++    +H A   GHL IVK L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL +   N
Sbjct:  315 PLHIACKKNHMRSMDLLLKHSASLEAVT-ESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVET-PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKAN 436          

HSP 2 Score: 75.0998 bits (183), Expect = 1.981e-16
Identity = 40/122 (32.79%), Postives = 63/122 (51.64%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+H+A   GHL IVK L+ +  +  A   +  E  +H A   GH E+ + L+  +  ++A   K    P+H A   GH E+ K+L+E K N ++       P+H+A   GHA+   +L D N
Sbjct:  348 PLHVAAFMGHLNIVKSLLQRGASPNASNVK-VETPLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDEN 467          

HSP 3 Score: 74.3294 bits (181), Expect = 3.754e-16
Identity = 37/126 (29.37%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ +V++L+++   ++A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  + KVL++K     A  +N   P+H+AC + H   + LL   + ++E
Sbjct:  216 PLHCAARNGHVRVVEILLDQGAPLQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLE 339          

HSP 4 Score: 73.559 bits (179), Expect = 8.310e-16
Identity = 37/118 (31.36%), Postives = 66/118 (55.93%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL+  K N ++         +H A  +GH +  ++L+D++     +  K    P+H AC+ G +++ ++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  414 PLHCAARMGHKELVKLLMEHKANPDSAT-TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFT-PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 529          

HSP 5 Score: 73.1738 bits (178), Expect = 1.178e-15
Identity = 36/119 (30.25%), Postives = 67/119 (56.30%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+     +N    +H   ++GH+ I  +L+ Q   + A   +    P+H AC  G++++ K L++++ ++ +       P+H A  +GH +IVTLL 
Sbjct:  579 PLHLASQEGQPDMVALLISKQANVNLGN-KNGLTPLHLVAQEGHVGIADMLVKQGASVYA-ASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 695          

HSP 6 Score: 71.633 bits (174), Expect = 3.878e-15
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  +VK+L++K     A R  N    +H AC++ H+  + LL+     +EA+       P+H A   GHL I K L+++  +  A  +    P+H+A   GH E+   L   N  ++ +
Sbjct:  282 PLHVAAHCGHHRMVKVLLDKGAKANA-RALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLT-PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAK 407          

HSP 7 Score: 71.2478 bits (173), Expect = 5.496e-15
Identity = 32/124 (25.81%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   + ++      + P+H A  +GH +  ++L+++             P+H+AC  G  ++V LL +   N
Sbjct:  381 PLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDS-ATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGAN 502          

HSP 8 Score: 66.2402 bits (160), Expect = 2.671e-13
Identity = 35/127 (27.56%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+ACK G +++V+LL+ +  N  A   +N    +H A    +L++VKLL+ +     +   +     +H A ++  LE+A  L++   N  +  +  + P+HLA   G  ++V LL     N+ +
Sbjct:  480 PLHVACKYGKVDVVELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNL 604          

HSP 9 Score: 63.5438 bits (153), Expect = 2.226e-12
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L++VKLL++K  +  +   +N   A+H A +Q  LE+   L+    + + E++ G     P+H A ++G  ++  +LI K+ N+     N L P+HL    GH  I  +L
Sbjct:  513 PLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGI---TPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADML 628          

HSP 10 Score: 60.4622 bits (145), Expect = 3.065e-11
Identity = 32/124 (25.81%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A K   LE+   L+    N  +   Q     +H A ++G  ++V LLI +  ++  +G K    P+H   ++GH+ IA +L+++  ++ A       P+H+AC  G+ ++V  L     ++
Sbjct:  547 LHIAAKQNQLEVASSLLQYGANANSESLQGIT-PLHLASQEGQPDMVALLISKQANVN-LGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHV 668          

HSP 11 Score: 51.9878 bits (123), Expect = 2.192e-8
Identity = 37/156 (23.72%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI+                ++ D        +N             P+H A    +L +A++L+ +  N+     N + P+H+A  RG+  +V LL D    I+ +
Sbjct:   55 PLYMAAQENHLEVVKYLLEHGAN-QSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAK 209          

HSP 12 Score: 51.9878 bits (123), Expect = 2.414e-8
Identity = 28/118 (23.73%), Postives = 59/118 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I  +L+ +  ++ A   +     +H AC  G++++VK L+ Q   + +   +    P+H A ++GH +I  +L++          N   P+ +A   G+  ++ +L
Sbjct:  612 PLHLVAQEGHVGIADMLVKQGASVYAAS-RMGYTPLHVACHYGNIKMVKFLLQQQAHVNS-KTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 727          

HSP 13 Score: 47.7506 bits (112), Expect = 6.795e-7
Identity = 27/127 (21.26%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  +V +L D    ++ +
Sbjct:  118 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLSVAQLLLNRGANVN-FTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAK 242          
BLAST of SMED30009057 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 75.485 bits (184), Expect = 1.595e-16
Identity = 40/124 (32.26%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+  + LL+    ++EA   ++    +H A   GHL IVK L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL +   N
Sbjct:  407 PLHIACKKNHMRSMDLLLKHSASLEAVT-ESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVET-PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKAN 528          

HSP 2 Score: 75.0998 bits (183), Expect = 1.994e-16
Identity = 40/122 (32.79%), Postives = 63/122 (51.64%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+H+A   GHL IVK L+ +  +  A   +  E  +H A   GH E+ + L+  +  ++A   K    P+H A   GH E+ K+L+E K N ++       P+H+A   GHA+   +L D N
Sbjct:  440 PLHVAAFMGHLNIVKSLLQRGASPNASNVK-VETPLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDEN 559          

HSP 3 Score: 74.3294 bits (181), Expect = 3.779e-16
Identity = 37/126 (29.37%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ +V++L+++   ++A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  + KVL++K     A  +N   P+H+AC + H   + LL   + ++E
Sbjct:  308 PLHCAARNGHVRVVEILLDQGAPLQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLE 431          

HSP 4 Score: 73.559 bits (179), Expect = 8.364e-16
Identity = 37/118 (31.36%), Postives = 66/118 (55.93%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL+  K N ++         +H A  +GH +  ++L+D++     +  K    P+H AC+ G +++ ++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  506 PLHCAARMGHKELVKLLMEHKANPDSAT-TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFT-PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 621          

HSP 5 Score: 71.633 bits (174), Expect = 3.903e-15
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  +VK+L++K     A R  N    +H AC++ H+  + LL+     +EA+       P+H A   GHL I K L+++  +  A  +    P+H+A   GH E+   L   N  ++ +
Sbjct:  374 PLHVAAHCGHHRMVKVLLDKGAKANA-RALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLT-PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAK 499          

HSP 6 Score: 71.2478 bits (173), Expect = 5.532e-15
Identity = 32/124 (25.81%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   + ++      + P+H A  +GH +  ++L+++             P+H+AC  G  ++V LL +   N
Sbjct:  473 PLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDS-ATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGAN 594          

HSP 7 Score: 66.2402 bits (160), Expect = 2.689e-13
Identity = 35/127 (27.56%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+ACK G +++V+LL+ +  N  A   +N    +H A    +L++VKLL+ +     +   +     +H A ++  LE+A  L++   N  +  +  + P+HLA   G  ++V LL     N+ +
Sbjct:  572 PLHVACKYGKVDVVELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNL 696          

HSP 8 Score: 63.5438 bits (153), Expect = 2.240e-12
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L++VKLL++K  +  +   +N   A+H A +Q  LE+   L+    + + E++ G     P+H A ++G  ++  +LI K+ N+     N L P+HL    GH  I  +L
Sbjct:  605 PLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGI---TPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADML 720          

HSP 9 Score: 60.4622 bits (145), Expect = 3.084e-11
Identity = 32/124 (25.81%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A K   LE+   L+    N  +   Q     +H A ++G  ++V LLI +  ++  +G K    P+H   ++GH+ IA +L+++  ++ A       P+H+AC  G+ ++V  L     ++
Sbjct:  639 LHIAAKQNQLEVASSLLQYGANANSESLQGIT-PLHLASQEGQPDMVALLISKQANVN-LGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHV 760          

HSP 10 Score: 59.3066 bits (142), Expect = 7.583e-11
Identity = 38/154 (24.68%), Postives = 70/154 (45.45%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIE------------------AWGINNLR-----------------PIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+     +N    +H   ++GH+ I  +L+ Q   + A   +    P+H AC  G++++ K L++++ ++                   +  +  LR                 P+H A  +GH +IVTLL 
Sbjct:  671 PLHLASQEGQPDMVALLISKQANVNLGN-KNGLTPLHLVAQEGHVGIADMLVKQGASVYA-ASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRVSVLLLDASYTGLFSVSVCTLRTFAFSFSLIFLFQLGYTPLHQAAQQGHTDIVTLLL 822          

HSP 11 Score: 56.225 bits (134), Expect = 9.013e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++   ++E    +    A+H A   G  ++V  LI+   ++ A   K  + P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V LL +
Sbjct:   82 LHLASKEGHVKMVLELLHNGIDLETTT-KKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFS-PLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 198          

HSP 12 Score: 51.9878 bits (123), Expect = 2.206e-8
Identity = 37/156 (23.72%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI+                ++ D        +N             P+H A    +L +A++L+ +  N+     N + P+H+A  RG+  +V LL D    I+ +
Sbjct:  147 PLYMAAQENHLEVVKYLLEHGAN-QSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAK 301          

HSP 13 Score: 47.7506 bits (112), Expect = 6.838e-7
Identity = 27/127 (21.26%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  +V +L D    ++ +
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLSVAQLLLNRGANVN-FTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAK 334          
BLAST of SMED30009057 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 75.485 bits (184), Expect = 1.618e-16
Identity = 40/124 (32.26%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+  + LL+    ++EA   ++    +H A   GHL IVK L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL +   N
Sbjct:  377 PLHIACKKNHMRSMDLLLKHSASLEAVT-ESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVET-PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKAN 498          

HSP 2 Score: 75.0998 bits (183), Expect = 2.022e-16
Identity = 40/122 (32.79%), Postives = 63/122 (51.64%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+H+A   GHL IVK L+ +  +  A   +  E  +H A   GH E+ + L+  +  ++A   K    P+H A   GH E+ K+L+E K N ++       P+H+A   GHA+   +L D N
Sbjct:  410 PLHVAAFMGHLNIVKSLLQRGASPNASNVK-VETPLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDEN 529          

HSP 3 Score: 74.3294 bits (181), Expect = 3.833e-16
Identity = 37/126 (29.37%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ +V++L+++   ++A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  + KVL++K     A  +N   P+H+AC + H   + LL   + ++E
Sbjct:  278 PLHCAARNGHVRVVEILLDQGAPLQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLE 401          

HSP 4 Score: 73.559 bits (179), Expect = 8.484e-16
Identity = 37/118 (31.36%), Postives = 66/118 (55.93%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL+  K N ++         +H A  +GH +  ++L+D++     +  K    P+H AC+ G +++ ++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  476 PLHCAARMGHKELVKLLMEHKANPDSAT-TAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFT-PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 591          

HSP 5 Score: 73.1738 bits (178), Expect = 1.202e-15
Identity = 36/119 (30.25%), Postives = 67/119 (56.30%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+     +N    +H   ++GH+ I  +L+ Q   + A   +    P+H AC  G++++ K L++++ ++ +       P+H A  +GH +IVTLL 
Sbjct:  641 PLHLASQEGQPDMVALLISKQANVNLGN-KNGLTPLHLVAQEGHVGIADMLVKQGASVYA-ASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 757          

HSP 6 Score: 71.633 bits (174), Expect = 3.959e-15
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  +VK+L++K     A R  N    +H AC++ H+  + LL+     +EA+       P+H A   GHL I K L+++  +  A  +    P+H+A   GH E+   L   N  ++ +
Sbjct:  344 PLHVAAHCGHHRMVKVLLDKGAKANA-RALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLT-PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAK 469          

HSP 7 Score: 71.2478 bits (173), Expect = 5.611e-15
Identity = 32/124 (25.81%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   + ++      + P+H A  +GH +  ++L+++             P+H+AC  G  ++V LL +   N
Sbjct:  443 PLHMAARAGHCEVAQFLLQNNAQVDA-KAKDDQTPLHCAARMGHKELVKLLMEHKANPDS-ATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGAN 564          

HSP 8 Score: 66.2402 bits (160), Expect = 2.727e-13
Identity = 35/127 (27.56%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+ACK G +++V+LL+ +  N  A   +N    +H A    +L++VKLL+ +     +   +     +H A ++  LE+A  L++   N  +  +  + P+HLA   G  ++V LL     N+ +
Sbjct:  542 PLHVACKYGKVDVVELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNL 666          

HSP 9 Score: 63.5438 bits (153), Expect = 2.272e-12
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L++VKLL++K  +  +   +N   A+H A +Q  LE+   L+    + + E++ G     P+H A ++G  ++  +LI K+ N+     N L P+HL    GH  I  +L
Sbjct:  575 PLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGI---TPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADML 690          

HSP 10 Score: 60.4622 bits (145), Expect = 3.128e-11
Identity = 32/124 (25.81%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A K   LE+   L+    N  +   Q     +H A ++G  ++V LLI +  ++  +G K    P+H   ++GH+ IA +L+++  ++ A       P+H+AC  G+ ++V  L     ++
Sbjct:  609 LHIAAKQNQLEVASSLLQYGANANSESLQGIT-PLHLASQEGQPDMVALLISKQANVN-LGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHV 730          

HSP 11 Score: 56.225 bits (134), Expect = 9.140e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++   ++E    +    A+H A   G  ++V  LI+   ++ A   K  + P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V LL +
Sbjct:   52 LHLASKEGHVKMVLELLHNGIDLETTT-KKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFS-PLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLIN 168          

HSP 12 Score: 51.9878 bits (123), Expect = 2.236e-8
Identity = 37/156 (23.72%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI+                ++ D        +N             P+H A    +L +A++L+ +  N+     N + P+H+A  RG+  +V LL D    I+ +
Sbjct:  117 PLYMAAQENHLEVVKYLLEHGAN-QSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAK 271          

HSP 13 Score: 51.9878 bits (123), Expect = 2.463e-8
Identity = 28/118 (23.73%), Postives = 59/118 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I  +L+ +  ++ A   +     +H AC  G++++VK L+ Q   + +   +    P+H A ++GH +I  +L++          N   P+ +A   G+  ++ +L
Sbjct:  674 PLHLVAQEGHVGIADMLVKQGASVYAAS-RMGYTPLHVACHYGNIKMVKFLLQQQAHVNS-KTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 789          

HSP 14 Score: 48.1358 bits (113), Expect = 5.142e-7
Identity = 32/120 (26.67%), Postives = 53/120 (44.17%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A ++G+LE     I    +I     QN    +H A ++GH+++V  L+    D+E    K  N  +H A   G  ++   LI    N+ A       P+++A    H E+V  L +   N
Sbjct:   22 AARSGNLEKALDHIKNGIDINIAN-QNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKK-GNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGAN 139          

HSP 15 Score: 47.7506 bits (112), Expect = 6.932e-7
Identity = 27/127 (21.26%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  +V +L D    ++ +
Sbjct:  180 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLSVAQLLLNRGANVN-FTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAK 304          
BLAST of SMED30009057 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 78.9518 bits (193), Expect = 1.222e-17
Identity = 39/119 (32.77%), Postives = 69/119 (57.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL++K+ N+     ++S   +H A ++GHL I ++L+     + A   +    P+H AC  G++++ K L++ + N+ A       P+H A  +GH +IVTLL 
Sbjct:  598 PLHLAAQEGHGDMVTLLLSKQANVHLGN-KSSLTPLHLASQEGHLNIAEVLVSHDASVAA-ETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 714          

HSP 2 Score: 72.7886 bits (177), Expect = 1.980e-15
Identity = 40/126 (31.75%), Postives = 66/126 (52.38%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK--KCNIEACRF----QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A   GH  + KLL++K  K N  A        N    +H AC++ H+ +++LL+     I+A+  K +  P+H A    H ++A  LI+ K  + A   ++  P+H A   GH E+V LL +
Sbjct:  327 PLHVAAHCGHHRVAKLLLDKGAKSNARALIIILFTTNGFTPLHIACKKSHIRVMELLLKSGASIDAVTEKVET-PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLE 451          

HSP 3 Score: 72.7886 bits (177), Expect = 2.119e-15
Identity = 38/121 (31.40%), Postives = 72/121 (59.50%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +H+A + G++ +V+LL+++   I+AC  ++    +H A   GH+ I ++L+D    I+A   K KN   PIH A +  HL+  ++L++ + +I+   +++L P+H+A   GH  +  LL D
Sbjct:  229 LHIASRRGNVIMVRLLLDRGAQIDACT-KDELTPLHCAVRNGHVRISEILLDHGAPIQA---KTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD 345          

HSP 4 Score: 72.4034 bits (176), Expect = 2.380e-15
Identity = 41/126 (32.54%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A    +L + +LL+N+  N+     QN   A+H A  +G++ +V+LL+D+   I+A   K +  P+H A   GH+ I+++L++    I+A   N L PIH+A    H + V LL     +I+
Sbjct:  195 PLHIAAHYENLNVAQLLLNRGANVNFTP-QNGISALHIASRRGNVIMVRLLLDRGAQIDACT-KDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID 318          

HSP 5 Score: 68.9366 bits (167), Expect = 4.268e-14
Identity = 42/157 (26.75%), Postives = 73/157 (46.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC--------------RFQNSERAI------------------HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A  + HL IV+LL+NK  +                  R  ++ RA+                  H A ++GH ++V LL+ +  ++  +G K    P+H A ++GHL IA+VL+    ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  532 PLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVH-LGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANV 687          

HSP 6 Score: 63.929 bits (154), Expect = 2.744e-12
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A ++GH E+V+LL+    + +          +H     GH +  +LL+D      ++  K    P+H A + G ++IA++L+E++ +  + G   L P+H+A +  H  IV LL +
Sbjct:  433 PLHCAARSGHYEMVRLLLENGAHPDPPT-TAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFT-PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 550          

HSP 7 Score: 60.077 bits (144), Expect = 4.812e-11
Identity = 31/118 (26.27%), Postives = 63/118 (53.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + GHL I ++L++   ++ A   +     +H AC  G++++VK L+    ++ A+  K    P+H A ++GH +I  +L++         +N + P+ +A   G+  +  +L
Sbjct:  631 PLHLASQEGHLNIAEVLVSHDASV-AAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVT-KLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 746          

HSP 8 Score: 58.9214 bits (141), Expect = 1.291e-10
Identity = 35/141 (24.82%), Postives = 64/141 (45.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PIH+A +  HL+ V+LL+  + +I+     +    +H A   GH  + KLL+D+       G K   R             P+H AC+K H+ + ++L++   +I+A       P+H+A    H ++   L      +  +
Sbjct:  294 PIHMASQGDHLDCVRLLLQYQAHIDDITLDHLT-PLHVAAHCGHHRVAKLLLDK-------GAKSNARALIIILFTTNGFTPLHIACKKSHIRVMELLLKSGASIDAVTEKVETPLHMAARAAHTDVADFLIQNKAKVNAR 426          

HSP 9 Score: 52.373 bits (124), Expect = 2.755e-8
Identity = 37/162 (22.84%), Postives = 65/162 (40.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-----------------------------------KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  + GH +  +LL++ +        +     +H A + G ++I +LL+++     +  GK    P+H A    HL I ++L+ K                                    CN  A  +  + P+HLA   GH ++VTLL     N+ +
Sbjct:  466 PLHLTTRGGHTQTAELLLDNQA-AHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNS-AGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCN--AQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHL 623          

HSP 10 Score: 52.373 bits (124), Expect = 2.891e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HL+ K GH+++V  L++K   +E    +    A+H A   G  ++V+ L++   ++ +   K    P++ A ++ HL + K L+E   N      +   P+ +A  +GH  +V  L
Sbjct:   35 LHLSSKEGHVKMVTELLHKDIVLETTT-KKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFT-PLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYL 149          

HSP 11 Score: 45.8246 bits (107), Expect = 5.377e-6
Identity = 27/120 (22.50%), Postives = 54/120 (45.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+L+     +    +I  C  QN   A+H + ++GH+++V  L+ +   +E    K  N  +H A   G  ++ + L+    N+ +       P+++A    H  +V  L +   N
Sbjct:    5 AARAGNLDKALDHLRNGVDINTCN-QNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKK-GNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGAN 122          
BLAST of SMED30009057 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 78.9518 bits (193), Expect = 1.222e-17
Identity = 39/119 (32.77%), Postives = 69/119 (57.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL++K+ N+     ++S   +H A ++GHL I ++L+     + A   +    P+H AC  G++++ K L++ + N+ A       P+H A  +GH +IVTLL 
Sbjct:  571 PLHLAAQEGHGDMVTLLLSKQANVHLGN-KSSLTPLHLASQEGHLNIAEVLVSHDASVAA-ETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 687          

HSP 2 Score: 72.4034 bits (176), Expect = 2.380e-15
Identity = 41/126 (32.54%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A    +L + +LL+N+  N+     QN   A+H A  +G++ +V+LL+D+   I+A   K +  P+H A   GH+ I+++L++    I+A   N L PIH+A    H + V LL     +I+
Sbjct:  200 PLHIAAHYENLNVAQLLLNRGANVNFTP-QNGISALHIASRRGNVIMVRLLLDRGAQIDACT-KDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID 323          

HSP 3 Score: 68.9366 bits (167), Expect = 4.268e-14
Identity = 42/157 (26.75%), Postives = 73/157 (46.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC--------------RFQNSERAI------------------HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A  + HL IV+LL+NK  +                  R  ++ RA+                  H A ++GH ++V LL+ +  ++  +G K    P+H A ++GHL IA+VL+    ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  505 PLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVH-LGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANV 660          

HSP 4 Score: 63.929 bits (154), Expect = 2.744e-12
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A ++GH E+V+LL+    + +          +H     GH +  +LL+D      ++  K    P+H A + G ++IA++L+E++ +  + G   L P+H+A +  H  IV LL +
Sbjct:  406 PLHCAARSGHYEMVRLLLENGAHPDPPT-TAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFT-PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 523          

HSP 5 Score: 61.2326 bits (147), Expect = 2.398e-11
Identity = 42/161 (26.09%), Postives = 69/161 (42.86%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID-------------------QHC----------------------DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+                     HC                       I+A+  K +  P+H A    H ++A  LI+ K  + A   ++  P+H A   GH E+V LL +
Sbjct:  266 PLHCAVRNGHVRISEILLDHGAPIQA-KTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCAIYYYYFFALTSFMELLLKSGASIDAVTEKVET-PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLE 424          

HSP 6 Score: 60.077 bits (144), Expect = 4.812e-11
Identity = 31/118 (26.27%), Postives = 63/118 (53.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + GHL I ++L++   ++ A   +     +H AC  G++++VK L+    ++ A+  K    P+H A ++GH +I  +L++         +N + P+ +A   G+  +  +L
Sbjct:  604 PLHLASQEGHLNIAEVLVSHDASV-AAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVT-KLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 719          

HSP 7 Score: 52.373 bits (124), Expect = 2.755e-8
Identity = 37/162 (22.84%), Postives = 65/162 (40.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-----------------------------------KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  + GH +  +LL++ +        +     +H A + G ++I +LL+++     +  GK    P+H A    HL I ++L+ K                                    CN  A  +  + P+HLA   GH ++VTLL     N+ +
Sbjct:  439 PLHLTTRGGHTQTAELLLDNQA-AHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNS-AGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCN--AQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHL 596          

HSP 8 Score: 52.373 bits (124), Expect = 2.891e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HL+ K GH+++V  L++K   +E    +    A+H A   G  ++V+ L++   ++ +   K    P++ A ++ HL + K L+E   N      +   P+ +A  +GH  +V  L
Sbjct:   40 LHLSSKEGHVKMVTELLHKDIVLETTT-KKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFT-PLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYL 154          

HSP 9 Score: 45.8246 bits (107), Expect = 5.377e-6
Identity = 27/120 (22.50%), Postives = 54/120 (45.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+L+     +    +I  C  QN   A+H + ++GH+++V  L+ +   +E    K  N  +H A   G  ++ + L+    N+ +       P+++A    H  +V  L +   N
Sbjct:   10 AARAGNLDKALDHLRNGVDINTCN-QNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKK-GNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGAN 127          
BLAST of SMED30009057 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 78.9518 bits (193), Expect = 1.223e-17
Identity = 39/119 (32.77%), Postives = 69/119 (57.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL++K+ N+     ++S   +H A ++GHL I ++L+     + A   +    P+H AC  G++++ K L++ + N+ A       P+H A  +GH +IVTLL 
Sbjct:  613 PLHLAAQEGHGDMVTLLLSKQANVHLGN-KSSLTPLHLASQEGHLNIAEVLVSHDASVAA-ETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 729          

HSP 2 Score: 72.4034 bits (176), Expect = 2.382e-15
Identity = 41/126 (32.54%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A    +L + +LL+N+  N+     QN   A+H A  +G++ +V+LL+D+   I+A   K +  P+H A   GH+ I+++L++    I+A   N L PIH+A    H + V LL     +I+
Sbjct:  242 PLHIAAHYENLNVAQLLLNRGANVNFTP-QNGISALHIASRRGNVIMVRLLLDRGAQIDACT-KDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHID 365          

HSP 3 Score: 68.9366 bits (167), Expect = 4.271e-14
Identity = 42/157 (26.75%), Postives = 73/157 (46.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC--------------RFQNSERAI------------------HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A  + HL IV+LL+NK  +                  R  ++ RA+                  H A ++GH ++V LL+ +  ++  +G K    P+H A ++GHL IA+VL+    ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  547 PLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVH-LGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANV 702          

HSP 4 Score: 63.929 bits (154), Expect = 2.746e-12
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A ++GH E+V+LL+    + +          +H     GH +  +LL+D      ++  K    P+H A + G ++IA++L+E++ +  + G   L P+H+A +  H  IV LL +
Sbjct:  448 PLHCAARSGHYEMVRLLLENGAHPDPPT-TAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFT-PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 565          

HSP 5 Score: 61.2326 bits (147), Expect = 2.400e-11
Identity = 42/161 (26.09%), Postives = 69/161 (42.86%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID-------------------QHC----------------------DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+                     HC                       I+A+  K +  P+H A    H ++A  LI+ K  + A   ++  P+H A   GH E+V LL +
Sbjct:  308 PLHCAVRNGHVRISEILLDHGAPIQA-KTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCAIYYYYFFALTSFMELLLKSGASIDAVTEKVET-PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLE 466          

HSP 6 Score: 60.077 bits (144), Expect = 4.815e-11
Identity = 31/118 (26.27%), Postives = 63/118 (53.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + GHL I ++L++   ++ A   +     +H AC  G++++VK L+    ++ A+  K    P+H A ++GH +I  +L++         +N + P+ +A   G+  +  +L
Sbjct:  646 PLHLASQEGHLNIAEVLVSHDASV-AAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVT-KLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 761          

HSP 7 Score: 52.373 bits (124), Expect = 2.757e-8
Identity = 37/162 (22.84%), Postives = 65/162 (40.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-----------------------------------KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  + GH +  +LL++ +        +     +H A + G ++I +LL+++     +  GK    P+H A    HL I ++L+ K                                    CN  A  +  + P+HLA   GH ++VTLL     N+ +
Sbjct:  481 PLHLTTRGGHTQTAELLLDNQA-AHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNS-AGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCN--AQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHL 638          

HSP 8 Score: 52.373 bits (124), Expect = 2.893e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HL+ K GH+++V  L++K   +E    +    A+H A   G  ++V+ L++   ++ +   K    P++ A ++ HL + K L+E   N      +   P+ +A  +GH  +V  L
Sbjct:   82 LHLSSKEGHVKMVTELLHKDIVLETTT-KKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFT-PLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYL 196          

HSP 9 Score: 45.8246 bits (107), Expect = 5.380e-6
Identity = 27/120 (22.50%), Postives = 54/120 (45.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+L+     +    +I  C  QN   A+H + ++GH+++V  L+ +   +E    K  N  +H A   G  ++ + L+    N+ +       P+++A    H  +V  L +   N
Sbjct:   52 AARAGNLDKALDHLRNGVDINTCN-QNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKK-GNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGAN 169          
BLAST of SMED30009057 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 78.9518 bits (193), Expect = 1.225e-17
Identity = 39/119 (32.77%), Postives = 69/119 (57.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL++K+ N+     ++S   +H A ++GHL I ++L+     + A   +    P+H AC  G++++ K L++ + N+ A       P+H A  +GH +IVTLL 
Sbjct:  596 PLHLAAQEGHGDMVTLLLSKQANVHLGN-KSSLTPLHLASQEGHLNIAEVLVSHDASVAA-ETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 712          

HSP 2 Score: 77.7962 bits (190), Expect = 3.663e-17
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+     I+ I       P+H A   GH  +AK+L++K     A  +N   P+H+AC + H  ++ LL  +  +I+
Sbjct:  233 PLHCAVRNGHVRISEILLDHGAPIQA-KTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLT-PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASID 356          

HSP 3 Score: 72.7886 bits (177), Expect = 2.295e-15
Identity = 38/120 (31.67%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A    H ++A  LI+ K  + A   ++  P+H A   GH E+V LL +
Sbjct:  332 PLHIACKKSHIRVMELLLKSGASIDAVT-ESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVET-PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLE 449          

HSP 4 Score: 69.3218 bits (168), Expect = 3.108e-14
Identity = 38/121 (31.40%), Postives = 67/121 (55.37%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            PIH+A +  HL+ V+LL+  + +I+     +    +H A   GH  + KLL+D+    +  A+ G     P+H AC+K H+ + ++L++   +I+A   + L P+H+A + GH  IV  L 
Sbjct:  266 PIHMASQGDHLDCVRLLLQYQAHIDDITLDHLT-PLHVAAHCGHHRVAKLLLDKGAKSNARALNG---FTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 382          

HSP 5 Score: 68.9366 bits (167), Expect = 4.278e-14
Identity = 42/157 (26.75%), Postives = 73/157 (46.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC--------------RFQNSERAI------------------HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A  + HL IV+LL+NK  +                  R  ++ RA+                  H A ++GH ++V LL+ +  ++  +G K    P+H A ++GHL IA+VL+    ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  530 PLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVH-LGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANV 685          

HSP 6 Score: 66.2402 bits (160), Expect = 3.533e-13
Identity = 35/128 (27.34%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + KLL++K     A R  N    +H AC++ H+ +++LL+     I+A+       P+H A   GHL I K L++K  +   + +    P+H+A    H ++   L      +  +
Sbjct:  299 PLHVAAHCGHHRVAKLLLDKGAKSNA-RALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLT-PLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNAR 424          

HSP 7 Score: 63.929 bits (154), Expect = 2.751e-12
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A ++GH E+V+LL+    + +          +H     GH +  +LL+D      ++  K    P+H A + G ++IA++L+E++ +  + G   L P+H+A +  H  IV LL +
Sbjct:  431 PLHCAARSGHYEMVRLLLENGAHPDPPT-TAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFT-PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 548          

HSP 8 Score: 60.077 bits (144), Expect = 4.823e-11
Identity = 31/118 (26.27%), Postives = 63/118 (53.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + GHL I ++L++   ++ A   +     +H AC  G++++VK L+    ++ A+  K    P+H A ++GH +I  +L++         +N + P+ +A   G+  +  +L
Sbjct:  629 PLHLASQEGHLNIAEVLVSHDASV-AAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVT-KLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 744          

HSP 9 Score: 52.373 bits (124), Expect = 2.761e-8
Identity = 37/162 (22.84%), Postives = 65/162 (40.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-----------------------------------KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  + GH +  +LL++ +        +     +H A + G ++I +LL+++     +  GK    P+H A    HL I ++L+ K                                    CN  A  +  + P+HLA   GH ++VTLL     N+ +
Sbjct:  464 PLHLTTRGGHTQTAELLLDNQA-AHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNS-AGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCN--AQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHL 621          

HSP 10 Score: 52.373 bits (124), Expect = 2.898e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HL+ K GH+++V  L++K   +E    +    A+H A   G  ++V+ L++   ++ +   K    P++ A ++ HL + K L+E   N      +   P+ +A  +GH  +V  L
Sbjct:    7 LHLSSKEGHVKMVTELLHKDIVLETTT-KKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFT-PLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYL 121          
BLAST of SMED30009057 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 78.9518 bits (193), Expect = 1.226e-17
Identity = 39/119 (32.77%), Postives = 69/119 (57.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL++K+ N+     ++S   +H A ++GHL I ++L+     + A   +    P+H AC  G++++ K L++ + N+ A       P+H A  +GH +IVTLL 
Sbjct:  634 PLHLAAQEGHGDMVTLLLSKQANVHLGN-KSSLTPLHLASQEGHLNIAEVLVSHDASVAA-ETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 750          

HSP 2 Score: 77.7962 bits (190), Expect = 3.664e-17
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+     I+ I       P+H A   GH  +AK+L++K     A  +N   P+H+AC + H  ++ LL  +  +I+
Sbjct:  271 PLHCAVRNGHVRISEILLDHGAPIQA-KTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLT-PLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASID 394          

HSP 3 Score: 72.7886 bits (177), Expect = 2.296e-15
Identity = 38/120 (31.67%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A    H ++A  LI+ K  + A   ++  P+H A   GH E+V LL +
Sbjct:  370 PLHIACKKSHIRVMELLLKSGASIDAVT-ESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVET-PLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLE 487          

HSP 4 Score: 69.3218 bits (168), Expect = 3.109e-14
Identity = 38/121 (31.40%), Postives = 67/121 (55.37%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            PIH+A +  HL+ V+LL+  + +I+     +    +H A   GH  + KLL+D+    +  A+ G     P+H AC+K H+ + ++L++   +I+A   + L P+H+A + GH  IV  L 
Sbjct:  304 PIHMASQGDHLDCVRLLLQYQAHIDDITLDHLT-PLHVAAHCGHHRVAKLLLDKGAKSNARALNG---FTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 420          

HSP 5 Score: 68.9366 bits (167), Expect = 4.280e-14
Identity = 42/157 (26.75%), Postives = 73/157 (46.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC--------------RFQNSERAI------------------HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A  + HL IV+LL+NK  +                  R  ++ RA+                  H A ++GH ++V LL+ +  ++  +G K    P+H A ++GHL IA+VL+    ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  568 PLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVH-LGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANV 723          

HSP 6 Score: 66.2402 bits (160), Expect = 3.534e-13
Identity = 35/128 (27.34%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + KLL++K     A R  N    +H AC++ H+ +++LL+     I+A+       P+H A   GHL I K L++K  +   + +    P+H+A    H ++   L      +  +
Sbjct:  337 PLHVAAHCGHHRVAKLLLDKGAKSNA-RALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLT-PLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNAR 462          

HSP 7 Score: 63.929 bits (154), Expect = 2.751e-12
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H A ++GH E+V+LL+    + +          +H     GH +  +LL+D      ++  K    P+H A + G ++IA++L+E++ +  + G   L P+H+A +  H  IV LL +
Sbjct:  469 PLHCAARSGHYEMVRLLLENGAHPDPPT-TAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFT-PLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 586          

HSP 8 Score: 60.077 bits (144), Expect = 4.825e-11
Identity = 31/118 (26.27%), Postives = 63/118 (53.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + GHL I ++L++   ++ A   +     +H AC  G++++VK L+    ++ A+  K    P+H A ++GH +I  +L++         +N + P+ +A   G+  +  +L
Sbjct:  667 PLHLASQEGHLNIAEVLVSHDASV-AAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVT-KLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 782          

HSP 9 Score: 52.373 bits (124), Expect = 2.762e-8
Identity = 37/162 (22.84%), Postives = 65/162 (40.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-----------------------------------KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+HL  + GH +  +LL++ +        +     +H A + G ++I +LL+++     +  GK    P+H A    HL I ++L+ K                                    CN  A  +  + P+HLA   GH ++VTLL     N+ +
Sbjct:  502 PLHLTTRGGHTQTAELLLDNQA-AHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNS-AGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCN--AQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHL 659          

HSP 10 Score: 52.373 bits (124), Expect = 2.899e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HL+ K GH+++V  L++K   +E    +    A+H A   G  ++V+ L++   ++ +   K    P++ A ++ HL + K L+E   N      +   P+ +A  +GH  +V  L
Sbjct:   45 LHLSSKEGHVKMVTELLHKDIVLETTT-KKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFT-PLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYL 159          

HSP 11 Score: 45.8246 bits (107), Expect = 5.391e-6
Identity = 27/120 (22.50%), Postives = 54/120 (45.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A + G+L+     +    +I  C  QN   A+H + ++GH+++V  L+ +   +E    K  N  +H A   G  ++ + L+    N+ +       P+++A    H  +V  L +   N
Sbjct:   15 AARAGNLDKALDHLRNGVDINTCN-QNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKK-GNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGAN 132          
BLAST of SMED30009057 vs. Ensembl Mouse
Match: Ripk4 (receptor-interacting serine-threonine kinase 4 [Source:MGI Symbol;Acc:MGI:1919638])

HSP 1 Score: 79.337 bits (194), Expect = 6.760e-18
Identity = 42/122 (34.43%), Postives = 68/122 (55.74%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+HLA + GH  + ++LI+   ++  C  Q ++  +H A E GH    +LL+ +    EA+  +     +H A + GHL   K+LIE+K ++ A G  N   +HLA  RGH+E+V  L  A+
Sbjct:  609 PLHLAAQRGHYRVARILIDLCSDVNICSLQ-AQTPLHVAAETGHTSTARLLLHRGAGKEALTSEGYT-ALHLAAQNGHLATVKLLIEEKADVMARGPLNQTALHLAAARGHSEVVEELVSAD 728          

HSP 2 Score: 67.3958 bits (163), Expect = 9.899e-14
Identity = 35/120 (29.17%), Postives = 67/120 (55.83%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            S P+H+A +     IV+LL+ +K ++ A + ++   A+HFA + G     +LL++++  +  +  + +  P+H AC+ G   I + L+ +  ++   G +   P+H A W+GH  IV LL
Sbjct:  474 STPLHMAVERKGRGIVELLLARKTSVNA-KDEDQWTALHFAAQNGDEASTRLLLEKNASVNEVDFEGRT-PMHVACQHGQENIVRTLLRRGVDVGLQGKDAWLPLHYAAWQGHLPIVKLL 591          

HSP 3 Score: 67.3958 bits (163), Expect = 1.249e-13
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKK-CNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            +H A +NG     +LL+ K  ++    F+     +H AC+ G   IV+ L+ +  D+  + GK    P+H+A  +GHL I K+L ++   ++ A  ++   P+HLA  RGH  +  +L D   ++ +
Sbjct:  510 LHFAAQNGDEASTRLLLEKNASVNEVDFE-GRTPMHVACQHGQENIVRTLLRRGVDV-GLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNI 634          

HSP 4 Score: 65.855 bits (159), Expect = 3.321e-13
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A   GHL IVKLL  +       +  +    +H A ++GH  + ++LID   D+  I       P+H A E GH   A++L+ +    EA        +HLA   GH   V LL +   ++
Sbjct:  575 PLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVN-ICSLQAQTPLHVAAETGHTSTARLLLHRGAGKEALTSEGYTALHLAAQNGHLATVKLLIEEKADV 698          
BLAST of SMED30009057 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 78.5666 bits (192), Expect = 1.367e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  308 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASID 431          

HSP 2 Score: 67.3958 bits (163), Expect = 1.244e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  671 PLHLAAQEGHTEMVALLLSKQANGNLGN-KSGLTPLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 787          

HSP 3 Score: 65.0846 bits (157), Expect = 6.840e-13
Identity = 39/120 (32.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K    A   ++  P+H A   GH  +V LL +
Sbjct:  407 PLHIACKKNHIRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 524          

HSP 4 Score: 62.7734 bits (151), Expect = 5.428e-12
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+    +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GH E+V LL     N
Sbjct:  572 PLHVAAKYGKVRLAELLLEHDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQAN 693          

HSP 5 Score: 61.2326 bits (147), Expect = 1.668e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  638 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHTEMVALLLSKQAN-GNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 763          

HSP 6 Score: 57.7658 bits (138), Expect = 2.810e-10
Identity = 33/128 (25.78%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L      ++
Sbjct:  605 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVD 728          

HSP 7 Score: 57.3806 bits (137), Expect = 3.217e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   82 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 198          

HSP 8 Score: 53.9138 bits (128), Expect = 6.952e-9
Identity = 34/122 (27.87%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + GH E+ K L+  K    A + ++ +  +H A   GH  +VKLL++     ++    G   + P+H A  +GH++ A  L+EK+ +          P+H+A   G   +  LL +
Sbjct:  473 PLHMAARAGHTEVAKYLLQNKAKANA-KAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG---HTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 590          

HSP 9 Score: 51.6026 bits (122), Expect = 3.840e-8
Identity = 29/118 (24.58%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      N   P+ +A   G+  +  +L
Sbjct:  704 PLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 819          

HSP 10 Score: 50.8322 bits (120), Expect = 8.073e-8
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   52 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 172          

HSP 11 Score: 48.9062 bits (115), Expect = 3.670e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  147 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETR 301          

HSP 12 Score: 44.669 bits (104), Expect = 9.333e-6
Identity = 27/127 (21.26%), Postives = 58/127 (45.67%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++   +     +    P+H A  +G++ + ++L+++   IE    + L P+H A   GH  I  +L D    I+ +
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLNVAQLLLNRGASVN-FTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAK 334          
BLAST of SMED30009057 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 78.5666 bits (192), Expect = 1.370e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  279 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASID 402          

HSP 2 Score: 67.3958 bits (163), Expect = 1.247e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  642 PLHLAAQEGHTEMVALLLSKQANGNLGN-KSGLTPLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 3 Score: 65.0846 bits (157), Expect = 6.791e-13
Identity = 39/120 (32.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K    A   ++  P+H A   GH  +V LL +
Sbjct:  378 PLHIACKKNHIRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 495          

HSP 4 Score: 62.7734 bits (151), Expect = 5.441e-12
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+    +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GH E+V LL     N
Sbjct:  543 PLHVAAKYGKVRLAELLLEHDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQAN 664          

HSP 5 Score: 61.2326 bits (147), Expect = 1.672e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  609 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHTEMVALLLSKQAN-GNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 734          

HSP 6 Score: 57.7658 bits (138), Expect = 2.817e-10
Identity = 33/128 (25.78%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L      ++
Sbjct:  576 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVD 699          

HSP 7 Score: 57.3806 bits (137), Expect = 3.225e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   53 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 169          

HSP 8 Score: 53.5286 bits (127), Expect = 7.104e-9
Identity = 34/122 (27.87%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + GH E+ K L+  K    A + ++ +  +H A   GH  +VKLL++     ++    G   + P+H A  +GH++ A  L+EK+ +          P+H+A   G   +  LL +
Sbjct:  444 PLHMAARAGHTEVAKYLLQNKAKANA-KAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG---HTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 561          

HSP 9 Score: 51.6026 bits (122), Expect = 3.849e-8
Identity = 29/118 (24.58%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      N   P+ +A   G+  +  +L
Sbjct:  675 PLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 790          

HSP 10 Score: 50.8322 bits (120), Expect = 8.092e-8
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   23 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 143          

HSP 11 Score: 48.9062 bits (115), Expect = 3.678e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  118 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETR 272          

HSP 12 Score: 44.669 bits (104), Expect = 9.354e-6
Identity = 27/127 (21.26%), Postives = 58/127 (45.67%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++   +     +    P+H A  +G++ + ++L+++   IE    + L P+H A   GH  I  +L D    I+ +
Sbjct:  181 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLNVAQLLLNRGASVN-FTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAK 305          
BLAST of SMED30009057 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 78.5666 bits (192), Expect = 1.370e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  279 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASID 402          

HSP 2 Score: 67.3958 bits (163), Expect = 1.248e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  642 PLHLAAQEGHTEMVALLLSKQANGNLGN-KSGLTPLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 3 Score: 65.0846 bits (157), Expect = 6.791e-13
Identity = 39/120 (32.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K    A   ++  P+H A   GH  +V LL +
Sbjct:  378 PLHIACKKNHIRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 495          

HSP 4 Score: 62.7734 bits (151), Expect = 5.442e-12
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+    +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GH E+V LL     N
Sbjct:  543 PLHVAAKYGKVRLAELLLEHDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQAN 664          

HSP 5 Score: 61.2326 bits (147), Expect = 1.672e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  609 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHTEMVALLLSKQAN-GNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 734          

HSP 6 Score: 57.7658 bits (138), Expect = 2.817e-10
Identity = 33/128 (25.78%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L      ++
Sbjct:  576 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVD 699          

HSP 7 Score: 57.3806 bits (137), Expect = 3.225e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   53 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 169          

HSP 8 Score: 53.5286 bits (127), Expect = 7.105e-9
Identity = 34/122 (27.87%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + GH E+ K L+  K    A + ++ +  +H A   GH  +VKLL++     ++    G   + P+H A  +GH++ A  L+EK+ +          P+H+A   G   +  LL +
Sbjct:  444 PLHMAARAGHTEVAKYLLQNKAKANA-KAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG---HTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 561          

HSP 9 Score: 51.6026 bits (122), Expect = 3.849e-8
Identity = 29/118 (24.58%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      N   P+ +A   G+  +  +L
Sbjct:  675 PLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 790          

HSP 10 Score: 50.8322 bits (120), Expect = 8.092e-8
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   23 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 143          

HSP 11 Score: 48.9062 bits (115), Expect = 3.679e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  118 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETR 272          

HSP 12 Score: 44.669 bits (104), Expect = 9.355e-6
Identity = 27/127 (21.26%), Postives = 58/127 (45.67%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++   +     +    P+H A  +G++ + ++L+++   IE    + L P+H A   GH  I  +L D    I+ +
Sbjct:  181 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLNVAQLLLNRGASVN-FTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAK 305          
BLAST of SMED30009057 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 78.5666 bits (192), Expect = 1.370e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  271 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASID 394          

HSP 2 Score: 67.3958 bits (163), Expect = 1.248e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  634 PLHLAAQEGHTEMVALLLSKQANGNLGN-KSGLTPLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 3 Score: 65.0846 bits (157), Expect = 6.727e-13
Identity = 39/120 (32.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K    A   ++  P+H A   GH  +V LL +
Sbjct:  370 PLHIACKKNHIRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 487          

HSP 4 Score: 62.7734 bits (151), Expect = 5.443e-12
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+    +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GH E+V LL     N
Sbjct:  535 PLHVAAKYGKVRLAELLLEHDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQAN 656          

HSP 5 Score: 61.2326 bits (147), Expect = 1.672e-11
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  601 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHTEMVALLLSKQAN-GNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 726          

HSP 6 Score: 57.7658 bits (138), Expect = 2.818e-10
Identity = 33/128 (25.78%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L      ++
Sbjct:  568 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVD 691          

HSP 7 Score: 57.3806 bits (137), Expect = 3.226e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   45 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 161          

HSP 8 Score: 53.9138 bits (128), Expect = 6.971e-9
Identity = 34/122 (27.87%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + GH E+ K L+  K    A + ++ +  +H A   GH  +VKLL++     ++    G   + P+H A  +GH++ A  L+EK+ +          P+H+A   G   +  LL +
Sbjct:  436 PLHMAARAGHTEVAKYLLQNKAKANA-KAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG---HTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 553          

HSP 9 Score: 51.6026 bits (122), Expect = 3.850e-8
Identity = 29/118 (24.58%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      N   P+ +A   G+  +  +L
Sbjct:  667 PLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 782          

HSP 10 Score: 50.8322 bits (120), Expect = 8.094e-8
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   15 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 135          

HSP 11 Score: 48.9062 bits (115), Expect = 3.679e-7
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  110 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETR 264          

HSP 12 Score: 44.669 bits (104), Expect = 9.357e-6
Identity = 27/127 (21.26%), Postives = 58/127 (45.67%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++   +     +    P+H A  +G++ + ++L+++   IE    + L P+H A   GH  I  +L D    I+ +
Sbjct:  173 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLNVAQLLLNRGASVN-FTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAK 297          
BLAST of SMED30009057 vs. UniProt/SwissProt
Match: sp|Q5UP11|YR848_MIMIV (Putative ankyrin repeat protein R848 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R848 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.916e-19
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
             + + LA KNGH+++VK L++K  N+      ++  A+ +A E GH E+VK L+DQ  DI       ++  + FA E GHLE+ K L++K  NI A    +   + LA   G+ EIV  L     N    
Sbjct:   10 DYALSLASKNGHIKVVKYLVSKDANVT----HDNNYAVRYASENGHFEVVKYLVDQGADIR----DCRDYAVRFASENGHLEVVKYLVDKGANIRAL---DDYAVCLASVNGYIEIVKYLVSQGANFRAD 128          

HSP 2 Score: 79.337 bits (194), Expect = 1.333e-17
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            ++ +  A +NGH E+VK L+++  +I  CR    + A+ FA E GHLE+VK L+D+  +I A+     +  +  A   G++EI K L+ +  N  A   +N   +  A   G+ E+V  L D   +I   
Sbjct:   40 NYAVRYASENGHFEVVKYLVDQGADIRDCR----DYAVRFASENGHLEVVKYLVDKGANIRAL----DDYAVCLASVNGYIEIVKYLVSQGANFRA---DNDYAVRFASENGYLEVVKFLVDQGADIRAD 158          

HSP 3 Score: 67.0106 bits (162), Expect = 4.059e-13
Identity = 44/126 (34.92%), Postives = 64/126 (50.79%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF----DANCNI 386
            LA  NG++EIVK L+++  N  A    +++ A+ FA E G+LE+VK L+DQ  DI A      +  I  A   GHLE+ K L+ +  +  +    N   I LA    H EI+        D N +I
Sbjct:  105 LASVNGYIEIVKYLVSQGANFRA----DNDYAVRFASENGYLEVVKFLVDQGADIRA----DDDYAIISASVYGHLEVIKFLMSQGADFRS---KNNASIKLAIKYKHPEIIEYFLTQCTDVNTDI 219          

HSP 4 Score: 49.2914 bits (116), Expect = 1.253e-6
Identity = 28/98 (28.57%), Postives = 48/98 (48.98%), Query Frame = 3
Query:   93 RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            R+ N + A+  A + GH+++VK L+ +  ++        N  + +A E GH E+ K L+++  +I       +R    A   GH E+V  L D   NI
Sbjct:    6 RYSN-DYALSLASKNGHIKVVKYLVSKDANVT----HDNNYAVRYASENGHFEVVKYLVDQGADIRDCRDYAVR---FASENGHLEVVKYLVDKGANI 95          
BLAST of SMED30009057 vs. UniProt/SwissProt
Match: sp|Q5UPE2|YL063_MIMIV (Putative ankyrin repeat protein L63 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L63 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 6.542e-18
Identity = 47/127 (37.01%), Postives = 69/127 (54.33%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            + LAC+NGHLEI++  +++  NI+      S  A+  A E GH+E+V+ LI    DI +      N  I +A E GHLE+ K L+++  NI A   NN R +  A  +GH E+V  L     NI  +
Sbjct:  345 VMLACQNGHLEIIRYFVSQGFNIKC----GSNCAVTIASENGHIEVVRYLISLGADINS----GNNYAIKYASENGHLEVVKYLVDQGANIRA---NNDRAVRFASRKGHLEVVKYLVSKGANIRAK 460          

HSP 2 Score: 77.7962 bits (190), Expect = 1.986e-16
Identity = 45/128 (35.16%), Postives = 72/128 (56.25%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            ++ I  A +NGHLE+VK L+++  NI A    N++RA+ FA  +GHLE+VK L+ +  +I A      +R +  A + GHLE+ K L+ +  +I+A    +   +  A   GH E+V  L     NI+
Sbjct:  402 NYAIKYASENGHLEVVKYLVDQGANIRA----NNDRAVRFASRKGHLEVVKYLVSKGANIRAK----DDRAVTLASQNGHLEVVKYLVSQGTDIKA---GDDYAVRWASRNGHLEVVKYLISQGANIK 518          

HSP 3 Score: 77.0258 bits (188), Expect = 3.519e-16
Identity = 45/124 (36.29%), Postives = 68/124 (54.84%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +  A + GHLE+VK L++K  NI A      +RA+  A + GHLE+VK L+ Q  DI+A      +  + +A   GHLE+ K LI +  NI+A   ++   +  A   GH E+V  L + N +I
Sbjct:  435 VRFASRKGHLEVVKYLVSKGANIRA----KDDRAVTLASQNGHLEVVKYLVSQGTDIKA----GDDYAVRWASRNGHLEVVKYLISQGANIKA---DDDYAVRWASLNGHLEVVKFLVNQNADI 547          

HSP 4 Score: 67.0106 bits (162), Expect = 8.838e-13
Identity = 46/117 (39.32%), Postives = 64/117 (54.70%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            IH A KNGH+ +VK L++K  NI A    N   AI FA E GHLE+V+ L+ +  DI A      N PI  A + GHL++ + L+    +I A   N+   + LA +  H E+V  L
Sbjct:  117 IH-AAKNGHIGVVKYLVSKGVNIGA----NDNCAIKFASENGHLEVVEYLVSKGADINAN----NNYPIEMASKNGHLKVVEYLVSLGVDIRA---NDDYVVGLAYYYDHHEVVDYL 221          

HSP 5 Score: 61.6178 bits (148), Expect = 6.959e-11
Identity = 37/98 (37.76%), Postives = 58/98 (59.18%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINN 308
            + LA +NGHLE+VK L+++  +I+A      + A+ +A   GHLE+VK LI Q  +I+A      +  + +A   GHLE+ K L+ +  +I A  INN
Sbjct:  465 VTLASQNGHLEVVKYLVSQGTDIKA----GDDYAVRWASRNGHLEVVKYLISQGANIKAD----DDYAVRWASLNGHLEVVKFLVNQNADIRA--INN 552          

HSP 6 Score: 60.077 bits (144), Expect = 2.355e-10
Identity = 32/89 (35.96%), Postives = 54/89 (60.67%), Query Frame = 3
Query:    9 HPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK 275
            + +  A +NGHLE+VK LI++  NI+A    + + A+ +A   GHLE+VK L++Q+ DI AI     N  + +A +  H ++ + LI +
Sbjct:  493 YAVRWASRNGHLEVVKYLISQGANIKA----DDDYAVRWASLNGHLEVVKFLVNQNADIRAI----NNYAVRWAHKNKHFDVVEYLISQ 573          
BLAST of SMED30009057 vs. UniProt/SwissProt
Match: sp|Q9ERK0|RIPK4_MOUSE (Receptor-interacting serine/threonine-protein kinase 4 OS=Mus musculus OX=10090 GN=Ripk4 PE=1 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 4.729e-17
Identity = 42/122 (34.43%), Postives = 68/122 (55.74%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+HLA + GH  + ++LI+   ++  C  Q ++  +H A E GH    +LL+ +    EA+  +     +H A + GHL   K+LIE+K ++ A G  N   +HLA  RGH+E+V  L  A+
Sbjct:  609 PLHLAAQRGHYRVARILIDLCSDVNICSLQ-AQTPLHVAAETGHTSTARLLLHRGAGKEALTSEGYT-ALHLAAQNGHLATVKLLIEEKADVMARGPLNQTALHLAAARGHSEVVEELVSAD 728          

HSP 2 Score: 67.3958 bits (163), Expect = 6.925e-13
Identity = 35/120 (29.17%), Postives = 67/120 (55.83%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            S P+H+A +     IV+LL+ +K ++ A + ++   A+HFA + G     +LL++++  +  +  + +  P+H AC+ G   I + L+ +  ++   G +   P+H A W+GH  IV LL
Sbjct:  474 STPLHMAVERKGRGIVELLLARKTSVNA-KDEDQWTALHFAAQNGDEASTRLLLEKNASVNEVDFEGRT-PMHVACQHGQENIVRTLLRRGVDVGLQGKDAWLPLHYAAWQGHLPIVKLL 591          

HSP 3 Score: 67.3958 bits (163), Expect = 8.736e-13
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKK-CNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            +H A +NG     +LL+ K  ++    F+     +H AC+ G   IV+ L+ +  D+  + GK    P+H+A  +GHL I K+L ++   ++ A  ++   P+HLA  RGH  +  +L D   ++ +
Sbjct:  510 LHFAAQNGDEASTRLLLEKNASVNEVDFE-GRTPMHVACQHGQENIVRTLLRRGVDV-GLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNI 634          

HSP 4 Score: 65.855 bits (159), Expect = 2.323e-12
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A   GHL IVKLL  +       +  +    +H A ++GH  + ++LID   D+  I       P+H A E GH   A++L+ +    EA        +HLA   GH   V LL +   ++
Sbjct:  575 PLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVN-ICSLQAQTPLHVAAETGHTSTARLLLHRGAGKEALTSEGYTALHLAAQNGHLATVKLLIEEKADV 698          
BLAST of SMED30009057 vs. UniProt/SwissProt
Match: sp|Q02357|ANK1_MOUSE (Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 9.586e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I ++L++    I+A + +N    IH A +  HL+ V+LL+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL     +I+
Sbjct:  271 PLHCAARNGHVRISEILLDHGAPIQA-KTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT-PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASID 394          

HSP 2 Score: 67.3958 bits (163), Expect = 8.729e-13
Identity = 35/119 (29.41%), Postives = 65/119 (54.62%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH E+V LL++K+ N      ++    +H   ++GH+ +  +LI     ++A   +    P+H A   G++++ K L++ + ++ A       P+H A  +GH +IVTLL 
Sbjct:  634 PLHLAAQEGHTEMVALLLSKQANGNLGN-KSGLTPLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 3 Score: 65.0846 bits (157), Expect = 4.706e-12
Identity = 39/120 (32.50%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+ACK  H+ +++LL+    +I+A   ++    +H A   GHL IVK L+ +         K +  P+H A   GH E+AK L++ K    A   ++  P+H A   GH  +V LL +
Sbjct:  370 PLHIACKKNHIRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVET-PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 487          

HSP 4 Score: 62.7734 bits (151), Expect = 3.807e-11
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-----WKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A K G + + +LL+    +  A   +N    +H A    +L+IVKLL+ +       GG      W    P+H A ++  +E+A+ L++   +  A  +  + P+HLA   GH E+V LL     N
Sbjct:  535 PLHVAAKYGKVRLAELLLEHDAHPNAAG-KNGLTPLHVAVHHNNLDIVKLLLPR-------GGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQAN 656          

HSP 5 Score: 61.2326 bits (147), Expect = 1.170e-10
Identity = 33/128 (25.78%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+ + L+    +  A   Q     +H A ++GH E+V LL+ +  +   +G K    P+H   ++GH+ +A VLI+    ++A       P+H+A   G+ ++V  L     ++  +
Sbjct:  601 PLHIAAKQNQIEVARSLLQYGGSANAESVQGVT-PLHLAAQEGHTEMVALLLSKQAN-GNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAK 726          

HSP 6 Score: 57.7658 bits (138), Expect = 1.971e-9
Identity = 33/128 (25.78%), Postives = 65/128 (50.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ--HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H+A  + +L+IVKLL+ +  +  +  + N    +H A +Q  +E+ + L+      + E++ G     P+H A ++GH E+  +L+ K+ N      + L P+HL    GH  +  +L      ++
Sbjct:  568 PLHVAVHHNNLDIVKLLLPRGGSPHSPAW-NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGV---TPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVD 691          

HSP 7 Score: 57.3806 bits (137), Expect = 2.257e-9
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++K+  +E    +    A+H A   G  E+V+ L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V  L +
Sbjct:   45 LHLASKEGHVKMVVELLHKEIILETTT-KKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLIN 161          

HSP 8 Score: 53.9138 bits (128), Expect = 4.876e-8
Identity = 34/122 (27.87%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + GH E+ K L+  K    A + ++ +  +H A   GH  +VKLL++     ++    G   + P+H A  +GH++ A  L+EK+ +          P+H+A   G   +  LL +
Sbjct:  436 PLHMAARAGHTEVAKYLLQNKAKANA-KAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG---HTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 553          

HSP 9 Score: 51.6026 bits (122), Expect = 2.693e-7
Identity = 29/118 (24.58%), Postives = 57/118 (48.31%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ +  +LI     ++A   +     +H A   G++++VK L+    D+ A   K    P+H A ++GH +I  +L++   +      N   P+ +A   G+  +  +L
Sbjct:  667 PLHLVSQEGHVPVADVLIKHGVTVDATT-RMGYTPLHVASHYGNIKLVKFLLQHQADVNA-KTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 782          

HSP 10 Score: 50.8322 bits (120), Expect = 5.662e-7
Identity = 31/123 (25.20%), Postives = 56/123 (45.53%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A ++G+L+     +    +I  C  QN    +H A ++GH+++V  L+ +   +E    K  N  +H A   G  E+ + L+    N+ A       P+++A    H E+V  L +   N  V
Sbjct:   15 AARSGNLDKALDHLRNGVDINTCN-QNGLNGLHLASKEGHVKMVVELLHKEIILETTTKK-GNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNV 135          

HSP 11 Score: 48.9062 bits (115), Expect = 2.574e-6
Identity = 36/156 (23.08%), Postives = 67/156 (42.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N +    ++    +  A +QGH  +V  LI+                ++ D        +N             P+H A    +L +A++L+ +  ++     N + P+H+A  RG+  +V LL D    IE +
Sbjct:  110 PLYMAAQENHLEVVKFLLENGAN-QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETR 264          
BLAST of SMED30009057 vs. UniProt/SwissProt
Match: sp|Q5UPA3|YL022_MIMIV (Putative ankyrin repeat protein L22 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L22 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.086e-16
Identity = 37/120 (30.83%), Postives = 64/120 (53.33%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            ++ + LA +NG++E+VK L++   +I+A    + + A+ +A   GHL +VK L+ Q  DI        +  +  A E GH ++ K L+ +  NI A   ++   + LA   GH E+V  L
Sbjct:    5 NYAVRLASRNGYIEVVKYLVSIGADIKA----DDDYAVKWASRYGHLRVVKFLVSQGADIRV----NNDYAVQLASENGHFDVVKYLVSQDANIRA---DDDYAVKLASVNGHVEVVKYL 113          

HSP 2 Score: 56.225 bits (134), Expect = 7.605e-10
Identity = 26/90 (28.89%), Postives = 47/90 (52.22%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK 275
             + +  A + GHL +VK L+++  +I      N++ A+  A E GH ++VK L+ Q  +I A      +  +  A   GH+E+ K L+ +
Sbjct:   35 DYAVKWASRYGHLRVVKFLVSQGADIRV----NNDYAVQLASENGHFDVVKYLVSQDANIRAD----DDYAVKLASVNGHVEVVKYLVSQ 116          

HSP 3 Score: 51.2174 bits (121), Expect = 5.213e-8
Identity = 27/92 (29.35%), Postives = 45/92 (48.91%), Query Frame = 3
Query:  114 AIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            A+  A   G++E+VK L+    DI+A      +  + +A   GHL + K L+ +  +I    +NN   + LA   GH ++V  L   + NI 
Sbjct:    7 AVRLASRNGYIEVVKYLVSIGADIKAD----DDYAVKWASRYGHLRVVKFLVSQGADIR---VNNDYAVQLASENGHFDVVKYLVSQDANIR 91          
BLAST of SMED30009057 vs. TrEMBL
Match: A0A1X7SJT3 (ANK_REP_REGION domain-containing protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.771e-28
Identity = 64/134 (47.76%), Postives = 93/134 (69.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLIN-KKCNIEACRFQNSERAIHFACEQGHLEIVK-LLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIE-KKCNIEAWGINNLRPIHLACWRGHAEIVT-LLFDANCNIEVQ 395
            P+H AC+NGH EIVK+L N  +CNIEA     ++R +H ACE G+++IV+ L+ID+HCD+ A   KW+N   P+H+ACEKG+ EI K+L    +CNIEA   +N RP+H AC  G+ +IV  L+ D +C++  +
Sbjct:   54 PLHYACENGHFEIVKILTNHPQCNIEAEDSYFNDRPLHKACESGNVDIVRHLVIDKHCDVNA---KWRNGYTPLHYACEKGYFEIVKILSNHPQCNIEAEDDSNDRPLHKACESGNVDIVCHLVIDKHCDVNAK 184          

HSP 2 Score: 108.612 bits (270), Expect = 1.951e-25
Identity = 61/134 (45.52%), Postives = 91/134 (67.91%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLIN-KKCNIEACRFQNSERAIHFACEQGHLEIV-KLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIE-KKCNIEAWGINNLRPIHLACWRGHAEIVT-LLFDANCNIEVQ 395
            P+H AC+ G+ EIVK+L N  +CNIEA    +++R +H ACE G+++IV  L+ID+HCD+ A   KW+N   P+H+ CEKG+ EI K+L    +CNIEA   +N RP+H AC  G+ +IV  L+ D +C++  +
Sbjct:  123 PLHYACEKGYFEIVKILSNHPQCNIEA-EDDSNDRPLHKACESGNVDIVCHLVIDKHCDVNA---KWRNGYTPLHYTCEKGYFEIVKILTNHPQCNIEAEDDSNDRPLHKACESGNVDIVCHLVIDKHCDVNAK 252          

HSP 3 Score: 97.0561 bits (240), Expect = 3.922e-21
Identity = 52/131 (39.69%), Postives = 88/131 (67.18%), Query Frame = 3
Query:   15 IHLACKNGHLEIVK-LLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ-HCDIEAIGGKWKNRPIHFACEKGHLEIAK-VLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD-ANCNIEVQ 395
             H AC++G+++IV+ L+I+K C++ A +++     +H+ACE GH EIVK+L +   C+IEA    + +RP+H ACE G+++I + ++I+K C++ A   N   P+H AC +G+ EIV +L +   CNIE +
Sbjct:   21 FHKACESGNVDIVRHLVIDKHCDVNA-KWRYGYTPLHYACENGHFEIVKILTNHPQCNIEAEDSYFNDRPLHKACESGNVDIVRHLVIDKHCDVNAKWRNGYTPLHYACEKGYFEIVKILSNHPQCNIEAE 150          
BLAST of SMED30009057 vs. TrEMBL
Match: A0A182H2I6 (Uncharacterized protein OS=Aedes albopictus OX=7160 GN=RP20_CCG020406 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.412e-27
Identity = 58/126 (46.03%), Postives = 82/126 (65.08%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH EIV++LI +K NI+A   +NS   +H AC  GH E+V++LI +  +++ +  + K  P+HFAC KGH E+ +VLI ++ NI A    N  P+HLAC  GH E+V +L     NIE
Sbjct: 1105 PLHLACLNGHKEIVEILIRRKANIDALALENS-TPLHLACLNGHKEVVEILIKEKANVDTLDER-KLTPLHFACHKGHKEVVEVLIREEANINALAFENFTPLHLACLNGHKEVVKVLIREKANIE 1228          

HSP 2 Score: 112.464 bits (280), Expect = 4.245e-26
Identity = 54/126 (42.86%), Postives = 83/126 (65.87%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH+E+V++LI +K N++A  F+N    +H AC  GH E+ ++LI +  ++ A+  K K  P+H AC+ GH E+ ++LI  K NI+A    N  P+HLAC+ GH E+V +L     NI+
Sbjct: 1567 PLHLACQNGHIEVVEILIREKSNVDALAFENC-TPLHLACLNGHKEVAEVLIRKVANVNALDEK-KATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANID 1690          

HSP 3 Score: 110.538 bits (275), Expect = 2.316e-25
Identity = 53/126 (42.06%), Postives = 82/126 (65.08%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NG  E+VK+L+ +K NI A   +N    +H AC  GH E+V++L+ +  +I+A+  K K+ P+H AC+ GH E+ ++LI +K NI+A  + N  P+HLAC  G  E+V +L     NI+
Sbjct: 1303 PLHLACQNGQKEVVKVLVREKININALALENC-TPLHLACLNGHKEVVEILVREKANIDALDEK-KSTPLHLACQNGHKEVVEILIREKANIDAMALENYTPLHLACLNGQKEVVEVLIREKANID 1426          

HSP 4 Score: 109.383 bits (272), Expect = 4.888e-25
Identity = 52/126 (41.27%), Postives = 84/126 (66.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+HLAC+NGH E+V++LI  K NI+A  F+N    +H AC  GH E+V++L+ +  +I+A+  +++N  P+H AC+ GH+E+ ++LI +K N++A    N  P+HLAC  GH E+  +L     N+
Sbjct: 1633 PLHLACQNGHKEVVEILIRGKANIDATDFRNC-TPLHLACFNGHKEVVEILVREKANIDAL--EFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANV 1755          

HSP 5 Score: 109.383 bits (272), Expect = 4.888e-25
Identity = 52/126 (41.27%), Postives = 84/126 (66.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+HLAC+NGH E+V++LI  K NI+A  F+N    +H AC  GH E+V++L+ +  +I+A+  +++N  P+H AC+ GH+E+ ++LI +K N++A    N  P+HLAC  GH E+  +L     N+
Sbjct: 1897 PLHLACQNGHKEVVEILIRGKANIDATDFRNC-TPLHLACFNGHKEVVEILVREKANIDAL--EFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANV 2019          

HSP 6 Score: 109.383 bits (272), Expect = 5.820e-25
Identity = 52/126 (41.27%), Postives = 82/126 (65.08%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH+E+V++LI +K N++A  F+N    +H AC  GH E+ ++LI +  ++ A+  K K  P+H AC+ GH E+ ++LI  K +I+A  + N  P+HLAC  G  E+V +L     NI+
Sbjct: 1699 PLHLACQNGHIEVVEILIREKSNVDALAFENC-TPLHLACLNGHKEVAEVLIRKVANVNALDEK-KATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANID 1822          

HSP 7 Score: 109.383 bits (272), Expect = 5.820e-25
Identity = 52/126 (41.27%), Postives = 82/126 (65.08%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH+E+V++LI +K N++A  F+N    +H AC  GH E+ ++LI +  ++ A+  K K  P+H AC+ GH E+ ++LI  K +I+A  + N  P+HLAC  G  E+V +L     NI+
Sbjct: 1963 PLHLACQNGHIEVVEILIREKSNVDALAFENC-TPLHLACLNGHKEVAEVLIRKVANVNALDEK-KATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANID 2086          

HSP 8 Score: 108.612 bits (270), Expect = 1.082e-24
Identity = 56/129 (43.41%), Postives = 82/129 (63.57%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            S P+HLAC NGH E+V++LI +K N++    +     +HFAC +GH E+V++LI +  +I A+   ++N  P+H AC  GH E+ KVLI +K NIEA  + N  P+HLAC  GH E+  +L     NI+
Sbjct: 1136 STPLHLACLNGHKEVVEILIKEKANVDTLD-ERKLTPLHFACHKGHKEVVEVLIREEANINALA--FENFTPLHLACLNGHKEVVKVLIREKANIEALALENCTPLHLACLNGHKEVAEILIREKSNID 1261          

HSP 9 Score: 106.301 bits (264), Expect = 6.201e-24
Identity = 56/127 (44.09%), Postives = 81/127 (63.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H AC  GH E+V++LI ++ NI A  F+N    +H AC  GH E+VK+LI +  +IEA+    +N  P+H AC  GH E+A++LI +K NI+A  + N  P+HLAC  GH E+V +L     NI+
Sbjct: 1171 PLHFACHKGHKEVVEVLIREEANINALAFENF-TPLHLACLNGHKEVVKVLIREKANIEALA--LENCTPLHLACLNGHKEVAEILIREKSNIDALELKNCTPLHLACLNGHNEVVEILIRGKANID 1294          

HSP 10 Score: 106.301 bits (264), Expect = 6.766e-24
Identity = 54/126 (42.86%), Postives = 79/126 (62.70%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIHLA  NGH E+V++L  +K NI+A  F+N    +H AC  GH E+ ++LI +  ++ A+  K K  P+H AC+ GH E+ ++LI  K NI+A    N  P+HLAC+ GH E+V +L     NI+
Sbjct: 1831 PIHLASLNGHKEVVEILAREKANIDATDFRNC-TPLHLACLNGHKEVAEVLIRKVANVNALDEK-KATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANID 1954          

HSP 11 Score: 104.375 bits (259), Expect = 2.681e-23
Identity = 54/126 (42.86%), Postives = 78/126 (61.90%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIHLA  NGH E+V++L  +K NI+A  F+N    +H AC  GH E+ ++LI +  ++ A+  K K  P+H AC+ GH E+ ++LI  K NI A    N  P+HLAC+ GH E+V +L     NI+
Sbjct: 1435 PIHLASLNGHKEVVEILAGEKANIDATDFRNC-TPLHLACLNGHKEVAEVLIRKVANVNALDEK-KATPLHLACQNGHKEVVEILIRGKANIGATDFRNCTPLHLACFNGHKEVVEILVREKANID 1558          

HSP 12 Score: 104.375 bits (259), Expect = 2.814e-23
Identity = 56/126 (44.44%), Postives = 79/126 (62.70%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+HLAC NGH E+VK+LI +K NIEA   +N    +H AC  GH E+ ++LI +  +I+A+  + KN  P+H AC  GH E+ ++LI  K NI+A  + N  P+HLAC  G  E+V +L     NI
Sbjct: 1204 PLHLACLNGHKEVVKVLIREKANIEALALENC-TPLHLACLNGHKEVAEILIREKSNIDAL--ELKNCTPLHLACLNGHNEVVEILIRGKANIDAMALENYTPLHLACQNGQKEVVKVLVREKINI 1326          

HSP 13 Score: 102.834 bits (255), Expect = 9.098e-23
Identity = 53/126 (42.06%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+V++L+ +K NI+A   + S   +H AC+ GH E+V++LI +  +I+A+  +    P+H AC  G  E+ +VLI +K NI+A  + N  PIHLA   GH E+V +L     NI+
Sbjct: 1336 PLHLACLNGHKEVVEILVREKANIDALDEKKS-TPLHLACQNGHKEVVEILIREKANIDAMALE-NYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAGEKANID 1459          

HSP 14 Score: 98.5969 bits (244), Expect = 3.386e-21
Identity = 50/127 (39.37%), Postives = 77/127 (60.63%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+ ++LI K  N+ A   +     +H AC+ GH E+V++LI    +I+A    ++N  P+H AC  GH E+ ++L+ +K NI+A    N  P+HLAC  GH E+V +L     N++
Sbjct: 1600 PLHLACLNGHKEVAEVLIRKVANVNALD-EKKATPLHLACQNGHKEVVEILIRGKANIDATD--FRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVD 1723          

HSP 15 Score: 98.5969 bits (244), Expect = 3.386e-21
Identity = 50/127 (39.37%), Postives = 77/127 (60.63%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+ ++LI K  N+ A   +     +H AC+ GH E+V++LI    +I+A    ++N  P+H AC  GH E+ ++L+ +K NI+A    N  P+HLAC  GH E+V +L     N++
Sbjct: 1864 PLHLACLNGHKEVAEVLIRKVANVNALD-EKKATPLHLACQNGHKEVVEILIRGKANIDATD--FRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVD 1987          

HSP 16 Score: 97.4413 bits (241), Expect = 7.720e-21
Identity = 52/128 (40.62%), Postives = 78/128 (60.94%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            S P+HLAC+NGH E+V++LI +K NI+A   +N    +H AC  G  E+V++LI +  +I+A+    +N  PIH A   GH E+ ++L  +K NI+A    N  P+HLAC  GH E+  +L     N+
Sbjct: 1367 STPLHLACQNGHKEVVEILIREKANIDAMALENY-TPLHLACLNGQKEVVEVLIREKANIDALA--LENCTPIHLASLNGHKEVVEILAGEKANIDATDFRNCTPLHLACLNGHKEVAEVLIRKVANV 1491          

HSP 17 Score: 96.6709 bits (239), Expect = 1.316e-20
Identity = 50/127 (39.37%), Postives = 76/127 (59.84%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+ ++LI K  N+ A   +     +H AC+ GH E+V++LI    +I A    ++N  P+H AC  GH E+ ++L+ +K NI+A    N  P+HLAC  GH E+V +L     N++
Sbjct: 1468 PLHLACLNGHKEVAEVLIRKVANVNALD-EKKATPLHLACQNGHKEVVEILIRGKANIGATD--FRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVD 1591          

HSP 18 Score: 96.2857 bits (238), Expect = 2.076e-20
Identity = 51/126 (40.48%), Postives = 74/126 (58.73%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+ ++LI K  N+ A   +     +H AC+ GH E+V++LI     I+A+  +    P+H AC  G  E+ +VLI +K NI+A  + N  PIHLA   GH E+V +L     NI+
Sbjct: 1732 PLHLACLNGHKEVAEVLIRKVANVNALD-EKKATPLHLACQNGHKEVVEILIRGKASIDAMALE-NYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANID 1855          

HSP 19 Score: 96.2857 bits (238), Expect = 2.076e-20
Identity = 51/126 (40.48%), Postives = 74/126 (58.73%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC NGH E+ ++LI K  N+ A   +     +H AC+ GH E+V++LI     I+A+  +    P+H AC  G  E+ +VLI +K NI+A  + N  PIHLA   GH E+V +L     NI+
Sbjct: 1996 PLHLACLNGHKEVAEVLIRKVANVNALD-EKKATPLHLACQNGHKEVVEILIRGKASIDAMALE-NYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANID 2119          

HSP 20 Score: 78.5666 bits (192), Expect = 3.086e-14
Identity = 42/105 (40.00%), Postives = 65/105 (61.90%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIH 323
            P+HLAC+NGH E+V++LI  K +I+A   +N    +H AC  G  E+V++LI +  +I+A+    +N  PIH A   GH E+ ++L  +K NI+A    N  P+H
Sbjct: 2029 PLHLACQNGHKEVVEILIRGKASIDAMALENY-TPLHLACLNGQKEVVEVLIREKANIDALA--LENCTPIHLASLNGHKEVVEILAREKANIDATDFRNCTPLH 2130          

HSP 21 Score: 69.3218 bits (168), Expect = 5.469e-11
Identity = 48/165 (29.09%), Postives = 74/165 (44.85%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK----------------------------------------KCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A +     IV+LLI K                                          NI A   +N    +H AC  GH EIV++LI +  +I+A+  +  + P+H AC  GH E+ ++LI++K N++      L P+H AC +GH E+V +L     NI
Sbjct: 1032 PLHVAIEGDQRHIVQLLIEKGADVNIRDAKLNFDTPISAKPPLQFSLFGNKGAEDIIIKGNVNIGALTHENC-TPLHLACLNGHKEIVEILIRRKANIDALALE-NSTPLHLACLNGHKEVVEILIKEKANVDTLDERKLTPLHFACHKGHKEVVEVLIREEANI 1194          
BLAST of SMED30009057 vs. TrEMBL
Match: A0A182GGY8 (ANK_REP_REGION domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG009719 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 2.149e-27
Identity = 58/126 (46.03%), Postives = 83/126 (65.87%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG-GKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+HLAC+NGH E+V++LI +K NI+A   + S   +H AC+ GH E+V++L+ +  +IEA+    W   P+H ACEKGH E+ K+LI +K NI+A       P+HLAC RGH E+V +    N NI
Sbjct: 1633 PLHLACQNGHKEVVEILIGEKANIDALTEKKS-TPLHLACQNGHKEVVEILVGEKANIEALNQDNW--TPLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFLRKNANI 1755          

HSP 2 Score: 113.62 bits (283), Expect = 1.923e-26
Identity = 60/129 (46.51%), Postives = 83/129 (64.34%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG-GKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            S P+HLAC+NGH E+V++LI  K NIEA   Q +   +HFAC  GH E+V++LI +  +IEA+    W   P+H +C  GH E+ ++LI +K NIEA   +N  P+HLAC  GH E+V +L     NIE
Sbjct: 1400 STPLHLACQNGHKEVVEILIRGKANIEALN-QVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNW--TPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIE 1525          

HSP 3 Score: 112.849 bits (281), Expect = 3.508e-26
Identity = 58/127 (45.67%), Postives = 83/127 (65.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH E+V++LI +K NI+A   + S   +H AC+ GH E+V++LI    +IEA+    W   P+HFAC  GH E+ ++LI +K NIEA   +N  P+HL+C  GH E+V +L     NIE
Sbjct: 1369 PLHLACQNGHKEVVEILIGEKANIDALTEKKS-TPLHLACQNGHKEVVEILIRGKANIEALNQVNW--TPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIE 1492          

HSP 4 Score: 112.079 bits (279), Expect = 6.652e-26
Identity = 53/126 (42.06%), Postives = 85/126 (67.46%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H AC+NGH E+V++L+ +K NI+A   +     +H AC+ GH E+V++LI +  +I+A+  K K+ P+H AC+ GH E+ ++L+ +K NIEA   +N  P+HLAC +GH E+V +L     NI+
Sbjct: 1600 PLHKACQNGHAEVVEILVKEKANIDALA-EKKFTPLHLACQNGHKEVVEILIGEKANIDALTEK-KSTPLHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANID 1723          

HSP 5 Score: 111.694 bits (278), Expect = 8.898e-26
Identity = 56/126 (44.44%), Postives = 83/126 (65.87%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH E+V++LI  K NIEA   Q++   +H AC+ GH E+V++L+ +  +I+A+  K K  P+H AC+ GH E+ ++LI +K NI+A       P+HLAC  GH E+V +L     NIE
Sbjct: 1567 PLHLACRNGHKEVVEILIRGKANIEALT-QDNWTPLHKACQNGHAEVVEILVKEKANIDALAEK-KFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANIE 1690          

HSP 6 Score: 109.383 bits (272), Expect = 5.838e-25
Identity = 54/127 (42.52%), Postives = 81/127 (63.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIG-GKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H AC NGH E+V++LI +K NIEA   Q++   +H +C  GH E+V++LI +  +IEA+    W   P+H AC+ GH E+ ++LI ++ NIEA    N  P+H+A W GH ++V +L     NIE
Sbjct: 1435 PLHFACHNGHKEVVEILIREKVNIEALN-QDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNW--TPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIE 1558          

HSP 7 Score: 108.997 bits (271), Expect = 6.371e-25
Identity = 55/128 (42.97%), Postives = 84/128 (65.62%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            S P+H AC+NG  E+V++LI +K NIEA   Q++   +H AC+ GH E+V++LI ++ +I+A+  + K  P+H AC +GH E  ++LI +K NI+A   N   P+H AC  GHAE+V +L     NI+
Sbjct: 1235 STPLHFACQNGRKEVVEILIREKANIEALN-QHNWTPLHLACQNGHKEVVEILIKENANIDAL-TEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANID 1360          

HSP 8 Score: 107.071 bits (266), Expect = 3.477e-24
Identity = 54/126 (42.86%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC  GH E V++LI +K NI+A   +N    +H AC+ GH E+V++L+ +  +I+A+  K K  P+H AC+ GH E+ ++LI +K NI+A       P+HLAC  GH E+V +L     NIE
Sbjct: 1303 PLHLACARGHKEAVQILIGEKANIDALD-ENKFTPLHKACQNGHAEVVEILVKEKANIDALAEK-KFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIE 1426          

HSP 9 Score: 106.301 bits (264), Expect = 6.101e-24
Identity = 51/120 (42.50%), Postives = 80/120 (66.67%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            S P+HLAC+NGH E+V++L+ +K NIEA   Q++   +H ACE+GH E+VK+LI +  +I+A+  K KN P+H AC++GH E+ ++ + K  NI A       P++LA   GH E+  ++
Sbjct: 1664 STPLHLACQNGHKEVVEILVGEKANIEALN-QDNWTPLHLACEKGHKEVVKILIGEKANIDALTQK-KNTPLHLACQRGHKEVVEIFLRKNANIAALDDEKCTPLNLASQNGHTEVEKIM 1781          

HSP 10 Score: 105.145 bits (261), Expect = 1.474e-23
Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-WKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC++GH E+V++LI ++ NIEA   Q +   +H A   GH ++V++LI +  +IEA+G + W   P+H AC  GH E+ ++LI  K NIEA   +N  P+H AC  GHAE+V +L     NI+
Sbjct: 1501 PLHLACQDGHKEVVEILIREEANIEALN-QYNYTPLHIASWNGHEKVVEILIREKANIEALGQENW--TPLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANID 1624          

HSP 11 Score: 104.76 bits (260), Expect = 2.173e-23
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+HLAC+NGH E+V++LI +  NI+A   ++    +H AC +GH E V++LI +  +I+A+    K  P+H AC+ GH E+ ++L+++K NI+A       P+HLAC  GH E+V +L     NI+
Sbjct: 1270 PLHLACQNGHKEVVEILIKENANIDALT-EHKYTPLHLACARGHKEAVQILIGEKANIDALDEN-KFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANID 1393          

HSP 12 Score: 98.9821 bits (245), Expect = 1.993e-21
Identity = 52/127 (40.94%), Postives = 79/127 (62.20%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H AC +G  E+V++LI  K NI+A   +N    +HFAC+ G  E+V++LI +  +IEA+    W   P+H AC+ GH E+ ++LI++  NI+A   +   P+HLAC RGH E V +L     NI+
Sbjct: 1204 PLHWACISGVKEVVEILIGGKANIDALA-KNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNW--TPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANID 1327          

HSP 13 Score: 96.2857 bits (238), Expect = 1.984e-20
Identity = 50/125 (40.00%), Postives = 78/125 (62.40%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            +H++  +GH  IV+LL++   N+ A    N    +H+AC  G  E+V++LI    +I+A+  K K+ P+HFAC+ G  E+ ++LI +K NIEA   +N  P+HLAC  GH E+V +L   N NI+
Sbjct: 1172 LHISASSGHRPIVELLLDNGANVNAAD-TNEYTPLHWACISGVKEVVEILIGGKANIDALA-KNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANID 1294          
BLAST of SMED30009057 vs. TrEMBL
Match: A0A1X7TAL4 (ANK_REP_REGION domain-containing protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.288e-27
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIV-KLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIVT-LLFDANCNIEVQ 395
            P+H AC+NGH EIVK+L N  +CNIEA RF +S++ +H ACE G++ IV  L+ID+HCD+ A  G++   P+ FACEKGH EI K L    +CNIEA    N RP+H+AC   +  IV  L+ D +C++  +
Sbjct:  673 PLHCACENGHFEIVKILTNHPQCNIEAERF-SSDKPLHKACESGNVNIVHHLVIDKHCDVNA-KGRFGYTPLLFACEKGHFEIVKFLTNHPQCNIEAENEYNDRPLHVACQSKNVNIVRHLVIDKHCDVNAK 802          

HSP 2 Score: 109.383 bits (272), Expect = 5.271e-25
Identity = 60/133 (45.11%), Postives = 87/133 (65.41%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIVK-LLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIV-TLLFDANCNIEVQ 395
            P+H ACK GH EIVK+L N  +CNIEA +  +S+R +H AC  G+++IV+ L+ID+HCD+ A    W    P+H ACE GH EI K+L    +CNIEA   ++ +P+H AC  G+  IV  L+ D +C++  +
Sbjct:  605 PLHDACKKGHFEIVKILTNHPECNIEA-KMDSSDRPLHIACASGNVDIVRHLVIDKHCDVNA--KDWSAYTPLHCACENGHFEIVKILTNHPQCNIEAERFSSDKPLHKACESGNVNIVHHLVIDKHCDVNAK 734          

HSP 3 Score: 98.5969 bits (244), Expect = 2.838e-21
Identity = 56/131 (42.75%), Postives = 83/131 (63.36%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ-HCDIEAIGGKWKNRPIHFACEKGHLEIAK-VLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD-ANCNIEVQ 395
            +H AC+ GH EIVK L N  +CNI+A R +N    +H AC++GH EIVK+L +   C+IEA      +RP+H AC  G+++I + ++I+K C++ A   +   P+H AC  GH EIV +L +   CNIE +
Sbjct:  572 LHYACQEGHFEIVKFLTNHPQCNIKA-RRRNGCTPLHDACKKGHFEIVKILTNHPECNIEAKMDS-SDRPLHIACASGNVDIVRHLVIDKHCDVNAKDWSAYTPLHCACENGHFEIVKILTNHPQCNIEAE 700          

HSP 4 Score: 68.5514 bits (166), Expect = 1.085e-10
Identity = 44/121 (36.36%), Postives = 66/121 (54.55%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQH-CDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+++AC   HL +V+ L  +  CNI +    N    +  A ++ HLEIVK  I+   CDI        + P+H AC  G L I + LI K +C+IEA      +P+H A  +GH EIV+++
Sbjct:  382 PLYMACIYNHLPVVEYLTGQPNCNINS---NNETHPLLAATDKEHLEIVKHFIESTGCDINVREKGTGSTPLHKACYNGSLSIVEYLISKPRCDIEAEDNEGNQPLHYAACQGHKEIVSIV 499          

HSP 5 Score: 66.6254 bits (161), Expect = 5.261e-10
Identity = 55/153 (35.95%), Postives = 83/153 (54.25%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIV-----KLLID--QHC----------DI-EAIGGKWKNR-PIHFACE-KGHLEIAK-VLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD-ANCNIEVQ 395
            S P+H AC NG L IV+ LI+K +C+IEA       + +H+A  QGH EIV     K+ ID    C          DI + +   +K+R  +  AC   G+++I + ++I+K C++ A G+N    +H AC  GH EIV  L +   CNI+ +
Sbjct:  447 STPLHKACYNGSLSIVEYLISKPRCDIEA-EDNEGNQPLHYAACQGHKEIVSIVGKKVSIDGLSKCMESAKQLTEPDIMDLLNNHYKDRISLLNACGISGNVDIVRHLVIDKHCDVNAKGMNGYTTLHYACQEGHFEIVKFLTNHPQCNIKAR 598          

HSP 6 Score: 60.077 bits (144), Expect = 8.803e-8
Identity = 39/102 (38.24%), Postives = 64/102 (62.75%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVK-LLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ-HCD-IEAIGGKWKNRPIHFAC-----EKGHLEIAKVLIE-KKCNIE 290
            P+H+AC++ ++ IV+ L+I+K C++ A + ++    +H+AC++G  EIVK L +   C+ IEA   K K RP+H A       K HL+I   L+E K C++E
Sbjct:  775 PLHVACQSKNVNIVRHLVIDKHCDVNA-KGKDCNTPLHYACQEGSFEIVKFLTNHLQCNTIEAENNKNK-RPLHSAISHRPLSKSHLDIVNYLVEVKGCSVE 874          

HSP 7 Score: 58.151 bits (139), Expect = 4.342e-7
Identity = 42/129 (32.56%), Postives = 65/129 (50.39%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLI-EKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN-CNIEVQ 395
            +H +C+ GHL IV+ L +    I   +   S  +IHFA E G + IVK L++       I  K+ N P++ AC   HL + + L  +  CNI +   N   P+  A  + H EIV    ++  C+I V+
Sbjct:  316 LHYSCRCGHLSIVEYLTSLP-QINYAKDTCSRTSIHFAAEFGQVHIVKYLVESCNHDINIEDKYGNTPLYMACIYNHLPVVEYLTGQPNCNINS--NNETHPLLAATDKEHLEIVKHFIESTGCDINVR 441          
BLAST of SMED30009057 vs. TrEMBL
Match: A0A1X7T0E5 (ANK_REP_REGION domain-containing protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 5.796e-27
Identity = 63/133 (47.37%), Postives = 88/133 (66.17%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIVK-LLIDQHCDIEAIGGKWKN-RPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIVT-LLFDANCNIEVQ 395
            P+H AC+ G+ EIVK+L N  +CNIEA    N +R +H ACE G ++IV+ L+ID+HCD+ A  G W N  P+HFAC+ GH EI K+L    +CNIEA    N RP+H AC  G+ EI+  L+ D +C++  +
Sbjct:  228 PLHHACQKGYFEIVKILTNHPQCNIEAKSLSN-DRPLHKACESGSIDIVRHLVIDKHCDVNAKAG-WSNYTPLHFACDNGHFEIVKLLTNHPQCNIEAEDDWNNRPLHKACESGNVEILRHLVIDKHCDVNAE 358          

HSP 2 Score: 107.457 bits (267), Expect = 2.276e-24
Identity = 57/132 (43.18%), Postives = 86/132 (65.15%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVK-LLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ-HCDIEAIGGKWKNRPIHFACEKGHLEIAK-VLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD-ANCNIEVQ 395
            P+H AC++G ++IV+ L+I+K C++ A    ++   +HFAC+ GH EIVKLL +   C+IEA    W NRP+H ACE G++EI + ++I+K C++ A G N   P+H AC  G+ E V  L +   CNIE +
Sbjct:  262 PLHKACESGSIDIVRHLVIDKHCDVNAKAGWSNYTPLHFACDNGHFEIVKLLTNHPQCNIEA-EDDWNNRPLHKACESGNVEILRHLVIDKHCDVNAEGRNGYTPLHAACLNGNFETVQFLTNHPQCNIEAK 392          

HSP 3 Score: 95.5153 bits (236), Expect = 3.423e-20
Identity = 60/135 (44.44%), Postives = 81/135 (60.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINK-KCNIEACRFQNSERAIHFACEQGHLEIVK-LLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACW-----RGHAEIVTLLFDAN-CNIE 389
            P+H AC NGH EIVKLL N  +CNIEA    N+ R +H ACE G++EI++ L+ID+HCD+ A  G+    P+H AC  G+ E  + L    +CNIEA   +N RP+ LA       + H +IV  L D   CN +
Sbjct:  297 PLHFACDNGHFEIVKLLTNHPQCNIEAEDDWNN-RPLHKACESGNVEILRHLVIDKHCDVNA-EGRNGYTPLHAACLNGNFETVQFLTNHPQCNIEAKSYHNKRPLDLAFHHKPPTKSHMDIVDYLIDVKGCNTQ 429          

HSP 4 Score: 85.5001 bits (210), Expect = 1.213e-16
Identity = 54/152 (35.53%), Postives = 83/152 (54.61%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINK-------KCNIEACRFQNSE------------RAIHFACEQGHLEIVK-LLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIVT-LLFDANCNIEVQ 395
            + P++ A   GH EIV +L  K       KC   A R    +            R++  AC+ G+++IV+ L+ID+HCD+ A  G     P+H AC+KG+ EI K+L    +CNIEA  ++N RP+H AC  G  +IV  L+ D +C++  +
Sbjct:  138 NQPLYYAACQGHKEIVSILGKKVREDGLSKCMESAKRLAEPDIMELLNDCYEDRRSLINACKSGNVDIVRHLVIDKHCDLNAKEGWSDYTPLHHACQKGYFEIVKILTNHPQCNIEAKSLSNDRPLHKACESGSIDIVRHLVIDKHCDVNAK 289          

HSP 5 Score: 55.4546 bits (132), Expect = 3.852e-6
Identity = 44/120 (36.67%), Postives = 64/120 (53.33%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERA----IHFACEQGHLEIVKLLIDQ-HCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK-KCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            AC NG  E V+LL+++    +   F+ S  A    +  A    +LEIVK LI+   CDI        + P+H AC  G L I + LI K +C+IEA      +P++ A  +GH EIV++L
Sbjct:   38 ACINGITECVQLLLSRSPPTDNI-FELSNNAGLSPLLAATNIKYLEIVKHLIESTDCDINVREEGTGSTPLHKACYNGSLSIVEYLISKPQCDIEAKDNEGNQPLYYAACQGHKEIVSIL 156          
BLAST of SMED30009057 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 76.6406 bits (187), Expect = 5.506e-17
Identity = 38/126 (30.16%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I+++L+++   I+A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  +AKVL++K        +N   P+H+AC + H  ++ LL   + ++E
Sbjct:  247 PLHCAARNGHVRIIEILLDQGAPIQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLE 370          

HSP 2 Score: 75.0998 bits (183), Expect = 2.057e-16
Identity = 40/124 (32.26%), Postives = 66/124 (53.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  HL ++ LL+    ++EA   ++    +H A   GHL IVK+L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++   +H A   GH E+V LL +   N
Sbjct:  346 PLHIACKKNHLRVMDLLLKHSASLEAVT-ESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVET-PLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKAN 467          

HSP 3 Score: 73.1738 bits (178), Expect = 9.058e-16
Identity = 36/119 (30.25%), Postives = 66/119 (55.46%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+     ++    +H   ++GH+ I  +L+ Q   + A   +    P+H AC  G++++ K L++++ N+         P+H A  +GH +IVTLL 
Sbjct:  610 PLHLAAQEGRADMVSLLISKQANVNLGN-KSGLTPLHLVAQEGHVGIADILVKQGASVYA-ATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLL 726          

HSP 4 Score: 71.633 bits (174), Expect = 3.130e-15
Identity = 40/122 (32.79%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+H+A   GHL IVK+L+ K  +  A   +  E  +H A   GH E+ + L+     ++A   K     +H A   GH E+ K+L+E K N  +       P+H+A   GH + V +L D N
Sbjct:  379 PLHVASFMGHLNIVKILLQKGASPNASNVK-VETPLHMASRAGHCEVAEFLLQNAAPVDA-KAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMN 498          

HSP 5 Score: 68.1662 bits (165), Expect = 4.448e-14
Identity = 37/117 (31.62%), Postives = 64/117 (54.70%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +H A + GH E+VKLL+  K N  +         +H A  +GH + V++L+D +     +  K    P+H A + G +++A++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  446 LHCAARMGHKELVKLLLEHKANPNSTT-TAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFT-PLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLL 560          

HSP 6 Score: 64.6994 bits (156), Expect = 8.693e-13
Identity = 33/125 (26.40%), Postives = 64/125 (51.20%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H+A K   +E+   L+    +  A   Q     +H A ++G  ++V LLI +  ++  +G K    P+H   ++GH+ IA +L+++  ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  577 PLHIASKQNQVEVASSLLQYGASANAESLQGVT-PLHLAAQEGRADMVSLLISKQANVN-LGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANV 699          

HSP 7 Score: 63.1586 bits (152), Expect = 2.546e-12
Identity = 30/124 (24.19%), Postives = 62/124 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ + ++H A   GH E+VKLL++   +  +      + P+H A  +GH +  ++L++              P+H+A   G  ++  LL +   N
Sbjct:  412 PLHMASRAGHCEVAEFLLQNAAPVDA-KAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTA-GHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGAN 533          

HSP 8 Score: 62.3882 bits (150), Expect = 5.795e-12
Identity = 33/127 (25.98%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G +++ +LL+ +  N  A   +N    +H A    +L++V LL+ +     +   +    P+H A ++  +E+A  L++   +  A  +  + P+HLA   G A++V+LL     N+ +
Sbjct:  511 PLHVAAKYGKVDVAELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVNLLVSKGGSPHS-AARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNL 635          

HSP 9 Score: 50.447 bits (119), Expect = 7.170e-8
Identity = 29/120 (24.17%), Postives = 60/120 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I  +L+ +  ++ A   +     +H AC  G++++VK L+ Q  ++    GK +    P+H A ++GH +I  +L++          N    + +A   G+  ++ +L
Sbjct:  643 PLHLVAQEGHVGIADILVKQGASVYAAT-RMGYTPLHVACHYGNIKMVKFLLQQQANVN---GKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVL 758          

HSP 10 Score: 49.2914 bits (116), Expect = 2.092e-7
Identity = 34/163 (20.86%), Postives = 67/163 (41.10%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIE---------------------------------ACRFQNSE--RAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N                                    C F  +     +H A    +L + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  I+ +L D    I+ +
Sbjct:  112 PLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVN-FTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAK 273          

HSP 11 Score: 44.2838 bits (103), Expect = 9.667e-6
Identity = 26/90 (28.89%), Postives = 47/90 (52.22%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN 284
            +HLA K GH+++V  L++    +E    +    A+H A   G  ++V  L++   ++ A   K    P++ A ++ HLE+ K L+E   N
Sbjct:   47 LHLASKEGHVKMVLELLHNGVVLETTT-KKGNTALHIAALAGQEQVVTELVNYGANVNAQSQK-GFTPLYMAAQENHLEVVKFLLENGAN 134          
BLAST of SMED30009057 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 76.2554 bits (186), Expect = 7.149e-17
Identity = 38/126 (30.16%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ ++++L+++   I+A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  +AKVL++K     A  +N   P+H+AC + H   + LL   + ++E
Sbjct:  250 PLHCAARNGHVRVIEILLDQGAPIQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLE 373          

HSP 2 Score: 74.3294 bits (181), Expect = 3.337e-16
Identity = 40/124 (32.26%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+  + LL+    ++EA   ++    +H A   GHL IVK L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL +   N
Sbjct:  349 PLHIACKKNHMRSMDLLLKHSASLEAVT-ESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVET-PLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKAN 470          

HSP 3 Score: 73.1738 bits (178), Expect = 8.879e-16
Identity = 40/122 (32.79%), Postives = 61/122 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDAN 377
            P+H+A   GHL IVK L+ +  +  A   +  E  +H A   GH E+ + L+     ++A   K    P+H A   GH E+ K+L+E K N ++       P+H+A   GHA    +L D N
Sbjct:  382 PLHVAAFMGHLNIVKSLLQRGASPNASNVK-VETPLHMAARAGHCEVAQFLLQNLAQVDA-KAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGN 501          

HSP 4 Score: 72.4034 bits (176), Expect = 1.455e-15
Identity = 38/118 (32.20%), Postives = 65/118 (55.08%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL+  K N ++         +H A  +GH    ++L+D +     +  K    P+H AC+ G +++A++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  448 PLHCAARMGHKELVKLLLEHKANPDSAT-TAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFT-PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 563          

HSP 5 Score: 66.2402 bits (160), Expect = 2.053e-13
Identity = 31/124 (25.00%), Postives = 62/124 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A + GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   + ++      + P+H A  +GH    ++L++              P+H+AC  G  ++  LL +   N
Sbjct:  415 PLHMAARAGHCEVAQFLLQNLAQVDA-KAKDDQTPLHCAARMGHKELVKLLLEHKANPDS-ATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGAN 536          

HSP 6 Score: 66.2402 bits (160), Expect = 2.516e-13
Identity = 35/127 (27.56%), Postives = 66/127 (51.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+ACK G +++ +LL+ +  N  A   +N    +H A    +L++VKLL+ +     +   +    P+H A ++  LE+A  L++   N  +  +  + P+HLA   G  ++V LL     N+ +
Sbjct:  514 PLHVACKYGKVDVAELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTA-RNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNL 638          

HSP 7 Score: 65.855 bits (159), Expect = 3.083e-13
Identity = 36/119 (30.25%), Postives = 59/119 (49.58%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A   GH  + K+L++K     A R  N    +H AC++ H+  + LL+     +EA+       P+H A   GHL I K L+++  +  A  +    P+H+A   GH E+   L 
Sbjct:  316 PLHVAAHCGHHRMAKVLLDKGAKANA-RALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLT-PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLL 432          

HSP 8 Score: 63.929 bits (154), Expect = 1.727e-12
Identity = 35/123 (28.46%), Postives = 66/123 (53.66%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIE---ACRFQ-NSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V LLI+K+ N+      R Q  +   +H   ++GH+ I  +L+ Q   + +   +     +H AC  G++++ K L++++ ++         P+H A  +GH +IVTLL 
Sbjct:  613 PLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYS-ASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLL 734          

HSP 9 Score: 55.4546 bits (132), Expect = 1.354e-9
Identity = 34/136 (25.00%), Postives = 66/136 (48.53%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-----PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD----ANCNIEV 392
            P+H+A K   LE+   L+    N  +   Q     +H A ++G  ++V LLI +  ++  +G K + +     P+H   ++GH+ IA +L+++  ++ +        +H+AC  G+ ++V  L       NC   V
Sbjct:  580 PLHIAAKQNQLEVASSLLQCGANANSESLQGIT-PLHLAAQEGRPDMVALLISKQGNVN-LGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRV 713          

HSP 10 Score: 54.299 bits (129), Expect = 3.078e-9
Identity = 33/119 (27.73%), Postives = 61/119 (51.26%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++   ++E    +    A+H A   G  ++V  L+    ++ A   K  + P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V LL +
Sbjct:   24 LHLASKEGHVKMVLELLHHGIDLETTT-KKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFS-PLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLIN 140          

HSP 11 Score: 51.9878 bits (123), Expect = 2.044e-8
Identity = 32/123 (26.02%), Postives = 61/123 (49.59%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNI-EACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR----PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I  +L+ +  ++  A R   +  A+H AC  G++++VK L+ Q   +       K R    P+H A ++GH +I  +L++          N   P+ +A   G+  ++ +L
Sbjct:  651 PLHLVAQEGHVGIADMLVKQGASVYSASRMGYT--ALHVACHYGNIKMVKFLLQQQAHVNC-----KTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 12 Score: 49.2914 bits (116), Expect = 1.862e-7
Identity = 31/125 (24.80%), Postives = 56/125 (44.80%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI+         GK +   +H A        A VL++   N +        P+H+A    +  +  LL +   N+
Sbjct:   89 PLYMAAQENHLEVVKFLLENGAN-QSLPTEDGFTPLAVALQQGHENVVALLINY-----GTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANV 207          

HSP 13 Score: 48.521 bits (114), Expect = 3.692e-7
Identity = 27/127 (21.26%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    +L + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  ++ +L D    I+ +
Sbjct:  152 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENLSVAQLLLNRGANVN-FTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAK 276          

HSP 14 Score: 47.3654 bits (111), Expect = 9.224e-7
Identity = 25/95 (26.32%), Postives = 43/95 (45.26%), Query Frame = 3
Query:   99 QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            QN    +H A ++GH+++V  L+    D+E    K  N  +H A   G  ++   L+    N+ A       P+++A    H E+V  L +   N
Sbjct:   18 QNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKK-GNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGAN 111          
BLAST of SMED30009057 vs. Ensembl Cavefish
Match: ripk4 (receptor interacting serine/threonine kinase 4 [Source:NCBI gene;Acc:103022568])

HSP 1 Score: 74.7146 bits (182), Expect = 2.356e-16
Identity = 44/157 (28.03%), Postives = 75/157 (47.77%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ---HCDIEAIGGKWK------------------------------NRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            PIH+AC +G   +V++L+++  +  A R ++   A+H A  QGHL IVKLL+ Q   + D + + G+                                + P+H A E GH   +++L++   +I++     L P+HLA W+GH   V +L +   N
Sbjct:  536 PIHVACHHGRENVVRVLLSRGADAHA-RGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDGRAPLHLASQRGQYRVARVLVDLGADVSITSACLSTPLHVAAETGHTSTSRLLLKHGADIQSRTAQGLTPLHLAAWKGHQATVRMLLEEGAN 691          

HSP 2 Score: 66.6254 bits (161), Expect = 1.582e-13
Identity = 37/131 (28.24%), Postives = 66/131 (50.38%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF-DANCNIEVQ 395
            S P+HLA +     + ++L+ +K      + ++    +HFA + G   + +LL+D+   +     + +  PIH AC  G   + +VL+ +  +  A G +    +HLA W+GH  IV LL   A  N++ Q
Sbjct:  467 STPLHLAVEKRLKSVAEILLGRKSTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRAATMNEADAQGRT-PIHVACHHGRENVVRVLLSRGADAHARGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQ 596          

HSP 3 Score: 49.2914 bits (116), Expect = 1.583e-7
Identity = 33/126 (26.19%), Postives = 62/126 (49.21%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC---DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN-IEAWGINNLRPIHLACWRGHAEIVTLLF 368
            LS P+H+A + GH    +LL+    +I++ R       +H A  +GH   V++L+++     D E +       P H A E GH ++ + L+++    +     + L P+H+A   G+ + + LL 
Sbjct:  633 LSTPLHVAAETGHTSTSRLLLKHGADIQS-RTAQGLTPLHLAAWKGHQATVRMLLEEGANPHDTEHL----LRTPCHLAAEGGHCQVMEELLQRWPEAVATPDESGLTPLHIAVRGGYRDAICLLL 753          
BLAST of SMED30009057 vs. Ensembl Cavefish
Match: ripk4 (receptor interacting serine/threonine kinase 4 [Source:NCBI gene;Acc:103022568])

HSP 1 Score: 73.9442 bits (180), Expect = 3.957e-16
Identity = 44/157 (28.03%), Postives = 75/157 (47.77%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQ---HCDIEAIGGKWK------------------------------NRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            PIH+AC +G   +V++L+++  +  A R ++   A+H A  QGHL IVKLL+ Q   + D + + G+                                + P+H A E GH   +++L++   +I++     L P+HLA W+GH   V +L +   N
Sbjct:  527 PIHVACHHGRENVVRVLLSRGADAHA-RGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDGRAPLHLASQRGQYRVARVLVDLGADVSITSACLSTPLHVAAETGHTSTSRLLLKHGADIQSRTAQGLTPLHLAAWKGHQATVRMLLEEGAN 682          

HSP 2 Score: 66.2402 bits (160), Expect = 2.435e-13
Identity = 37/131 (28.24%), Postives = 66/131 (50.38%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF-DANCNIEVQ 395
            S P+HLA +     + ++L+ +K      + ++    +HFA + G   + +LL+D+   +     + +  PIH AC  G   + +VL+ +  +  A G +    +HLA W+GH  IV LL   A  N++ Q
Sbjct:  458 STPLHLAVEKRLKSVAEILLGRKSTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRAATMNEADAQGRT-PIHVACHHGRENVVRVLLSRGADAHARGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQ 587          

HSP 3 Score: 50.447 bits (119), Expect = 7.572e-8
Identity = 34/133 (25.56%), Postives = 65/133 (48.87%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC---DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN-IEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            LS P+H+A + GH    +LL+    +I++ R       +H A  +GH   V++L+++     D E +       P H A E GH ++ + L+++    +     + L P+H+A   G+ + + LL     +I+
Sbjct:  624 LSTPLHVAAETGHTSTSRLLLKHGADIQS-RTAQGLTPLHLAAWKGHQATVRMLLEEGANPHDTEHL----LRTPCHLAAEGGHCQVMEELLQRWPEAVATPDESGLTPLHIAVRGGYRDAICLLLSHGADIK 751          
BLAST of SMED30009057 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 72.4034 bits (176), Expect = 1.755e-15
Identity = 34/126 (26.98%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H   ++GH ++V++L+++   I + + +N    +H A +  HL  V+LL+     ++ +   +    +H A   GH ++AKV+++KK N  A  +N   P+H+AC +   +++ LL     +I+
Sbjct:  251 PLHCGARSGHEQVVEMLLDRGAPILS-KTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLT-ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQ 374          

HSP 2 Score: 68.1662 bits (165), Expect = 4.913e-14
Identity = 36/122 (29.51%), Postives = 71/122 (58.20%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCD--IEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A +  HL  V+LL++ +  ++     +   A+H A   GH ++ K+++D+  +   +A+ G     P+H AC+K  +++ ++L++   +I+A   + L PIH+A + GH  IVT L +
Sbjct:  284 PLHMATQGDHLNCVQLLLHHEVPVDDVT-NDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNG---FTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMN 401          

HSP 3 Score: 63.5438 bits (153), Expect = 2.385e-12
Identity = 40/127 (31.50%), Postives = 65/127 (51.18%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF--DANCNI 386
            P+H+A K G +E+  LL+ K+   +A   ++    +H A    + ++  LL+DQ     A   K    P+H A +K  +EIA  L+E   +  A     + P+HLA   G+ ++VTLL   DA  N+
Sbjct:  515 PLHVAAKYGKIEVANLLLQKRAPPDASG-KSGLTPLHVAAHYDNQKVALLLLDQGASPHA-AAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINL 639          

HSP 4 Score: 58.151 bits (139), Expect = 1.499e-10
Identity = 35/127 (27.56%), Postives = 60/127 (47.24%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            PIH+A   GH  IV  L+N   +      +  E A+H A   G  E+VK L+     ++A   K    P+H +   G  EI + L++     ++   +   P+HLA   GH ++ ++L D   ++ +
Sbjct:  383 PIHVAAFMGHENIVTQLMNHGASPNTTNVR-GETALHMAARAGQTEVVKYLVQNGAYVDA-KSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGI 507          

HSP 5 Score: 56.6102 bits (135), Expect = 5.914e-10
Identity = 33/129 (25.58%), Postives = 68/129 (52.71%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+E+V  LI     ++A   +    A+H A   G  ++VK L+    ++ A   + +N   P++ A ++ HL++ + L++   +      +   P+ +A  +GH ++V+LL + +   +V+
Sbjct:   25 LHLASKEGHVEVVTQLIKMGATVDAAT-KKGNTALHIASLAGQSDVVKELVTNGANVNA---QSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR 149          

HSP 6 Score: 55.0694 bits (131), Expect = 1.868e-9
Identity = 30/98 (30.61%), Postives = 48/98 (48.98%), Query Frame = 3
Query:   78 NIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +I  C  QN   A+H A ++GH+E+V  LI     ++A   K  N  +H A   G  ++ K L+    N+ A   N   P+++A    H ++V  L D
Sbjct:   13 DINICN-QNGLNALHLASKEGHVEVVTQLIKMGATVDA-ATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLD 108          

HSP 7 Score: 53.9138 bits (128), Expect = 5.252e-9
Identity = 31/117 (26.50%), Postives = 57/117 (48.72%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +H+A   GH ++ K++++KK N  A +  N    +H AC++  +++++LL+     I+A+       PIH A   GH  I   L+    +     +     +H+A   G  E+V  L
Sbjct:  318 LHVAAHCGHYKVAKVIVDKKANPNA-KALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLT-PIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYL 432          

HSP 8 Score: 53.5286 bits (127), Expect = 6.432e-9
Identity = 33/159 (20.75%), Postives = 74/159 (46.54%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLI-------------------------------DQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HL++V+ L++   + ++   ++    +  A +QGH ++V LL+                               D + D+E+  G     P+H A   G++ +A +L+ +   ++    N++ P+H+A  RG++ +V LL +    I+ +
Sbjct:   90 PLYMAAQENHLDVVRYLLDNGSS-QSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSG---FTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAK 244          

HSP 9 Score: 53.5286 bits (127), Expect = 6.558e-9
Identity = 30/128 (23.44%), Postives = 61/128 (47.66%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +EI   L+    +  A   Q     +H A ++G++++V LL+ +   I  +  K    P+H A ++  + +A+VL+     ++        P+H+AC  G+ ++V  L      +  +
Sbjct:  581 PLHIAAKKNQMEIATTLLEYGADTNAITRQGIS-PLHLASQEGNVDMVTLLMARDATIN-LCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAK 706          

HSP 10 Score: 52.373 bits (124), Expect = 1.420e-8
Identity = 29/118 (24.58%), Postives = 58/118 (49.15%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            +H+A + G  E+VK L+     ++A + ++ +  +H +   G  EI++ L+ QH             P+H A  +GH ++A +L+++  ++         P+H+A   G  E+  LL 
Sbjct:  417 LHMAARAGQTEVVKYLVQNGAYVDA-KSKDDQTPLHISSRLGKPEIIQQLL-QHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLL 532          

HSP 11 Score: 50.8322 bits (120), Expect = 6.400e-8
Identity = 30/121 (24.79%), Postives = 61/121 (50.41%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +H+A +    +   LL+    N +    ++    +H A   G++ +  LL+++     A+  K +N   P+H A ++G+  + K+L+E+   I+A   + L P+H     GH ++V +L D
Sbjct:  153 LHIAARKDDTKAAALLLQNDHNADV-ESKSGFTPLHIAAHYGNINVATLLLNRGA---AVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLD 269          
BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 76.2554 bits (186), Expect = 1.597e-17
Identity = 41/122 (33.61%), Postives = 66/122 (54.10%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            LAC  GH ++V +LI +  NIE  R +     +  A   GH+ +V+LL+D   DIEA   + K+ P+  AC  G  E+ ++LI +  N E   +++  P+ LA   G+  I+ +L +A   I
Sbjct:  939 LACAGGHEDLVTVLIARGANIEH-RDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEI 1059          

HSP 2 Score: 70.4774 bits (171), Expect = 1.754e-15
Identity = 40/130 (30.77%), Postives = 65/130 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHF----ACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+  A   GH+E+ +LL+ +   I     +  E A+  AC +GHLE+V+ L++   D E      K   +H     AC  GH+E+A++L+E    +     +   P+ LA   GH E+  LL +   N+E
Sbjct:  236 PLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEH-----KTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLE 360          

HSP 3 Score: 69.707 bits (169), Expect = 2.635e-15
Identity = 40/125 (32.00%), Postives = 63/125 (50.40%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LAC  GHLE+V+ L+    + E  +      A+  AC  GH+E+ +LL++    +      +++ P+  A   GH+E+A +LIE+  N+E        P+  A   GH E+V LL     NI  Q
Sbjct:  273 LACYKGHLEMVRFLLEAGADQEH-KTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFES-PLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQ 395          

HSP 4 Score: 68.5514 bits (166), Expect = 7.872e-15
Identity = 41/126 (32.54%), Postives = 63/126 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+  A + GH E+V LL+ +  NI A   +  E A+  AC  G LE+   LI    D+E +G    + P+  A ++GHLE+ K L+    N+ A        +  AC  GH ++  LL  A  ++E
Sbjct:  369 PLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADLE-LGC---STPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLE 490          

HSP 5 Score: 62.7734 bits (151), Expect = 7.453e-13
Identity = 40/128 (31.25%), Postives = 66/128 (51.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+ LAC  G  E+V+LLI +  N E  R  +    +  A   G++ I+K+L++   +I +  G K    P+  A   GH    K+L++   +I A    N    + LAC++G  E+V+LL +   N+E
Sbjct: 1003 PLSLACSGGRQEVVELLIARGANKEH-RNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVE 1129          

HSP 6 Score: 61.6178 bits (148), Expect = 2.099e-12
Identity = 39/128 (30.47%), Postives = 63/128 (49.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIH---FACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+ LA   G++ I+K+L+N    I +     S+  I     A   GH   VKLL+D   DI A     +N  +  AC +G  E+  +L+E+K N+E      L P+  A   G+AE+  +L +   ++
Sbjct: 1036 PLSLAASGGYVNIIKILLNAGAEINS--RTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADV 1161          

HSP 7 Score: 59.6918 bits (143), Expect = 9.773e-12
Identity = 41/136 (30.15%), Postives = 64/136 (47.06%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNL--RPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+ LA   GH+ +V+LL++   +IEA   +  +  +  AC  G  E+V+LLI       A G   ++R      P+  A   G++ I K+L+     I +   + L   P+ LA   GH   V LL D   +I  Q
Sbjct:  969 PLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLI-------ARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQ 1097          

HSP 8 Score: 56.225 bits (134), Expect = 1.382e-10
Identity = 33/125 (26.40%), Postives = 59/125 (47.20%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LAC  G+ E+ ++L+    N+E    +     +  A   G ++IV+LL+    D+ A      N  + +AC  G++++ +VL+E   N+E    +   P+  A   GH E+  LL +    I   
Sbjct:  139 LACSAGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGADVNAQSST-GNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTH 262          

HSP 9 Score: 55.8398 bits (133), Expect = 2.075e-10
Identity = 40/160 (25.00%), Postives = 70/160 (43.75%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIE-------------AIGG---------------------KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+ LA  NGH   VKLL++   +I A    N   A+  AC QG  E+V LL+++  ++E             A GG                       ++  +  A +KGH +  +++I +  +I+        P+ LA   GH ++V LL ++  +++
Sbjct: 1071 PLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVD 1230          

HSP 10 Score: 53.1434 bits (126), Expect = 1.806e-9
Identity = 29/119 (24.37%), Postives = 63/119 (52.94%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+  A   G+ E+ ++L+ +  ++ A    +S + A+  A ++GH +  +L+I +   I+ +  K  N P+  A   GHL++ ++L+    +++A     + P+  A  +GH ++V  L
Sbjct: 1138 PLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHID-VRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYL 1255          

HSP 11 Score: 52.7582 bits (125), Expect = 2.607e-9
Identity = 29/122 (23.77%), Postives = 60/122 (49.18%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            AC +G +  V+ L+ +  ++     +  E  +  AC  G+ E+ ++L+  H ++E  G K    P+  A   G ++I ++L+    ++ A        +  AC  G+ ++V +L +A  N+E
Sbjct:  107 ACSDGDVNAVRKLLGEGRSVHETT-EEGESLLCLACSAGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLE 227          

HSP 12 Score: 43.5134 bits (101), Expect = 3.661e-6
Identity = 32/121 (26.45%), Postives = 51/121 (42.15%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIE--AIGGKWK-----------------------NRP--------IHFACEKGHLEIAKVLI 269
            S P+  A + GHLE+VK L+    N+ A      + A+ +ACE GH ++  LL+    D+E  + GG+                         NR         +  AC  GHL + ++L+
Sbjct:  431 STPLMEAAQEGHLELVKYLLAAGANVHATT-ATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLL 550          

HSP 13 Score: 43.5134 bits (101), Expect = 3.804e-6
Identity = 22/57 (38.60%), Postives = 28/57 (49.12%), Query Frame = 3
Query:  225 FACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
             AC  GH ++  VLI +  NIE        P+ LA   GH  +V LL D   +IE Q
Sbjct:  939 LACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQ 995          
BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Match: ankdd1a (ankyrin repeat and death domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-070615-8])

HSP 1 Score: 75.485 bits (184), Expect = 2.885e-17
Identity = 48/130 (36.92%), Postives = 71/130 (54.62%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINK----KCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            IHLA K+GH+  ++ L+ K      +    +  N   A+H A E GHLE V++L+D    CDI A+  +  N  +H A + GH +IAK+LI++  +I A       P+HLA    +  IVT L +A  NI
Sbjct:  173 IHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVRMLLDCEAICDINALNNESMN-ALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEANI 301          

HSP 2 Score: 70.0922 bits (170), Expect = 2.019e-15
Identity = 45/128 (35.16%), Postives = 68/128 (53.12%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLIN--KKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            +HLA + GHLE V++L++    C+I A   + S  A+H A + GH +I KLLID+  DI AI  K +  P+H A +  +  I   L+E + NI      N   +H+A   G  E   LL  A  ++ +
Sbjct:  211 LHLAAEGGHLECVRMLLDCEAICDINALNNE-SMNALHMAAKHGHEQIAKLLIDETIDINAISTK-QASPLHLAVQNNYPIIVTFLVEAEANINLVDCRNQTALHIAAETGFQESADLLLAAGTDLTL 336          

HSP 3 Score: 56.6102 bits (135), Expect = 1.116e-10
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            A +N   E+ KLL  KK  ++A   ++ + A+HFA   G  + VKLL+++  +++  G K+   P+  A   GHLE+ K L+     + +   N L  +H A  RGH E V    ++
Sbjct:   11 AKRNDVDEMEKLL--KKVKVDAKNMRD-QTALHFAAGSGQEDAVKLLLERKANVDK-GDKYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLNILHCAAQRGHVETVKYTLES 123          

HSP 4 Score: 53.9138 bits (128), Expect = 8.096e-10
Identity = 41/132 (31.06%), Postives = 65/132 (49.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHF-ACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN-----IEAWGINNLRPIHLACWRGHAEIVTLLFD--ANCNI 386
            +H A + GH+E VK  +    ++      N+E+  +  A E G L +++LL+D  C  +   G+ KN  IH A + GH+   + L+EK        + +   N    +HLA   GH E V +L D  A C+I
Sbjct:  105 LHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQDVSDGE-KNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVRMLLDCEAICDI 235          

HSP 5 Score: 51.2174 bits (121), Expect = 7.046e-9
Identity = 35/114 (30.70%), Postives = 59/114 (51.75%), Query Frame = 3
Query:    9 HPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKW--KNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGH 344
            +P+ +A   GHLE++K L+N    + + + +N    +H A ++GH+E VK  ++   D+  I GK   +  P   A E G L + ++L++ KC  +         IHLA   GH
Sbjct:   70 NPLLIAAWFGHLEVLKRLVNGGGRV-SSQNKNGLNILHCAAQRGHVETVKYTLESLEDL-GIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQDVSDGEKNTAIHLAAKSGH 181          

HSP 6 Score: 46.2098 bits (108), Expect = 3.895e-7
Identity = 35/129 (27.13%), Postives = 60/129 (46.51%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLR--PIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H A  +G  + VKLL+ +K N++    +     +  A   GHLE++K L++    + +      N  +H A ++GH+E  K  +E   ++   G  N    P  LA   G   ++ LL D  C  +V 
Sbjct:   39 LHFAAGSGQEDAVKLLLERKANVDKGD-KYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLN-ILHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQDVS 165          

HSP 7 Score: 42.743 bits (99), Expect = 6.230e-6
Identity = 25/97 (25.77%), Postives = 47/97 (48.45%), Query Frame = 3
Query:  108 ERAIHFACEQGHL-EIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            E+  H A ++  + E+ KLL     D + +  +     +HFA   G  +  K+L+E+K N++      + P+ +A W GH E++  L +    +  Q
Sbjct:    4 EKEFHEAAKRNDVDEMEKLLKKVKVDAKNMRDQ---TALHFAAGSGQEDAVKLLLERKANVDKGDKYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQ 97          
BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007954.1 (pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gene:ENSPMAG00000007165.1 transcript:ENSPMAT00000007954.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.4774 bits (171), Expect = 1.362e-15
Identity = 41/127 (32.28%), Postives = 68/127 (53.54%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR----PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            +H A  NG+LE+V LLI+K  N+ A   +   RA+H+A   GH E+VK+L+    D+      WK++    P+H A   GH+ + ++L+  K  +  +       +H+AC  G   +V+ L DA  +
Sbjct:   51 LHHAAFNGNLEMVNLLISKGANVNAMD-KRERRAVHWAGYMGHTEVVKMLLSNGADV-----NWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNTALHVACHNGQDAVVSELLDAGAD 171          

HSP 2 Score: 54.6842 bits (130), Expect = 5.664e-10
Identity = 35/119 (29.41%), Postives = 58/119 (48.74%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A  N  ++    LI    N+     +    A+H A   G+LE+V LLI +  ++ A+  K + R +H+A   GH E+ K+L+    ++         P+H A   GH  +V LL 
Sbjct:   17 PLHVASANRAIKCADALIPLLSNVNVSD-RAGRTALHHAAFNGNLEMVNLLISKGANVNAMD-KRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLL 133          

HSP 3 Score: 43.8986 bits (102), Expect = 3.170e-6
Identity = 33/115 (28.70%), Postives = 54/115 (46.96%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNI-EACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR---PIHFACEKGHLEIA-KVLIEKKCNIEAWGINNLRPIHLACWRG 341
            P+H A  +GH+ +V+LL++ K  + E   F N+  A+H AC  G   +V  L+D   D     G    R    +HFA    H  I  ++L+    ++ A   +   P+H+    G
Sbjct:  116 PLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNT--ALHVACHNGQDAVVSELLDAGAD----AGHRNERGFTALHFAAASTHGAICLELLVNHHADVNAQSYDGKSPLHMTAIHG 224          
BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.9366 bits (167), Expect = 4.940e-15
Identity = 40/123 (32.52%), Postives = 62/123 (50.41%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A +NG+LE +   +    +I  C  QN   A+H A ++GH+++V  L+ +  +I+    K  N  +H A   G  +I +VL+E   NI A   N   P+++A    H EIV  L D   N  V
Sbjct:   17 AARNGNLERLMDHLKGGMDINTCN-QNGLNALHLASKEGHVDVVTELLKREAEIDT-ATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSV 137          

HSP 2 Score: 67.0106 bits (162), Expect = 2.216e-14
Identity = 33/125 (26.40%), Postives = 68/125 (54.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH +++++L+ +   I A + +N    +H A +  HL  V+LL+ +   ++ +   +   P+H A   GH  +AK+L+++     A  +N   P+H+AC +   +++ LL     +I
Sbjct:  281 PLHCAARSGHEKVIEMLLERDAPILA-KTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLT-PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASI 403          

HSP 3 Score: 67.0106 bits (162), Expect = 2.796e-14
Identity = 39/127 (30.71%), Postives = 64/127 (50.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G LE+  LL+ K  + +  + +N    +H A    H ++  LL+D+     +   K    P+H A +K  LE+ KVL+E            + P+HLA   GHA++V LL   + +I +
Sbjct:  545 PLHIASKYGRLEVATLLLEKYASADP-KGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTA-KNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISL 669          

HSP 4 Score: 66.6254 bits (161), Expect = 3.394e-14
Identity = 36/120 (30.00%), Postives = 62/120 (51.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G LE+VK L+     +   + +  +  +H A   G  EIV+LL+ +  D  A      + P++FA  +GH ++A +L+E   +I         P+H+A   G  E+ TLL +
Sbjct:  446 PLHMASRAGQLEVVKTLLQSGARV-GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTAS-GSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLE 563          

HSP 5 Score: 64.3142 bits (155), Expect = 2.494e-13
Identity = 36/125 (28.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A +  HL  V+LL+ ++  ++     +    +H A   GH  + KLL+D+       GG+   R      P+H AC+K  +++ ++L++   +I     + L PIH+A + GH  +V LL 
Sbjct:  314 PLHMATQGEHLACVQLLLGRQAPVDDVTL-DYLTPLHVAAHCGHYRVAKLLLDR-------GGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL 430          

HSP 6 Score: 63.1586 bits (152), Expect = 5.104e-13
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIE-ACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+++V  L+ ++  I+ A +  N+  A+H A   G  +IV++L++   +I A   + +N   P++ A ++ HLEI K L++   N      +   P+ +A  +GH ++V LL +++   +V+
Sbjct:   47 LHLASKEGHVDVVTELLKREAEIDTATKVGNT--ALHIAALAGQTDIVRVLVEFGANINA---QSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVR 171          

HSP 7 Score: 60.8474 bits (146), Expect = 3.210e-12
Identity = 34/128 (26.56%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + KLL+++     A R  N    +H AC++  +++++LL+     I          PIH A   GHL + ++L++   +  A  +    P+H+A   G  E+V  L  +   + V+
Sbjct:  347 PLHVAAHCGHYRVAKLLLDRGGEPNA-RALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLT-PIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVK 472          

HSP 8 Score: 58.5362 bits (140), Expect = 2.117e-11
Identity = 37/123 (30.08%), Postives = 63/123 (51.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR----PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A   G++ +  LL+N    ++    +N    +H A ++G+  +VKLL+D+   I+      K R    P+H A   GH ++ ++L+E+   I A   N L P+H+A    H   V LL 
Sbjct:  215 PLHIAAHYGNMNVATLLLNLGAAVDFTA-RNGITPLHVAAKRGNNNMVKLLLDRGAKIDT-----KTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLL 331          

HSP 9 Score: 56.6102 bits (135), Expect = 9.475e-11
Identity = 30/119 (25.21%), Postives = 57/119 (47.90%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL+ +  +I     +    A+H A ++  + + ++L     D+     K    P+H A   G++++   L+  +  + A   N   P+H A  +GH  +V  L 
Sbjct:  644 PLHLAAQEGHADMVALLLGRHASISLAT-KGGLTAMHLAAQEDKVNVAEILTKHKADVNTTT-KLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760          

HSP 10 Score: 50.447 bits (119), Expect = 1.453e-8
Identity = 38/164 (23.17%), Postives = 71/164 (43.29%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------------------QHCDIEAIGGKWK-NR-------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLEIVK L++   N ++   ++    +  A +QGH ++V LL++                            QH     +  K   N+       P+H A   G++ +A +L+     ++    N + P+H+A  RG+  +V LL D    I+ +
Sbjct:  112 PLYMAAQENHLEIVKFLLDNGAN-QSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTK 274          

HSP 11 Score: 43.1282 bits (100), Expect = 4.887e-6
Identity = 30/133 (22.56%), Postives = 61/133 (45.86%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACR----FQNSERA---IHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A +        LL+  + N +        Q +E     +H A   G++ +  LL++     D  A  G     P+H A ++G+  + K+L+++   I+    + L P+H A   GH +++ +L + +  I
Sbjct:  175 LHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGI---TPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPI 304          
BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.1662 bits (165), Expect = 9.578e-15
Identity = 40/123 (32.52%), Postives = 62/123 (50.41%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            A +NG+LE +   +    +I  C  QN   A+H A ++GH+++V  L+ +  +I+    K  N  +H A   G  +I +VL+E   NI A   N   P+++A    H EIV  L D   N  V
Sbjct:   18 AARNGNLERLMDHLKGGMDINTCN-QNGLNALHLASKEGHVDVVTELLKREAEIDT-ATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSV 138          

HSP 2 Score: 66.2402 bits (160), Expect = 4.174e-14
Identity = 33/125 (26.40%), Postives = 68/125 (54.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+H A ++GH +++++L+ +   I A + +N    +H A +  HL  V+LL+ +   ++ +   +   P+H A   GH  +AK+L+++     A  +N   P+H+AC +   +++ LL     +I
Sbjct:  274 PLHCAARSGHEKVIEMLLERDAPILA-KTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLT-PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASI 396          

HSP 3 Score: 65.855 bits (159), Expect = 5.972e-14
Identity = 40/128 (31.25%), Postives = 63/128 (49.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIE-ACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G LE+  LL+ K  + +     QN    +H A    H ++  LL+D+     +   K    P+H A +K  LE+ KVL+E            + P+HLA   GHA++V LL   + +I +
Sbjct:  538 PLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTA-KNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISL 664          

HSP 4 Score: 65.855 bits (159), Expect = 6.773e-14
Identity = 36/120 (30.00%), Postives = 62/120 (51.67%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A + G LE+VK L+     +   + +  +  +H A   G  EIV+LL+ +  D  A      + P++FA  +GH ++A +L+E   +I         P+H+A   G  E+ TLL +
Sbjct:  439 PLHMASRAGQLEVVKTLLQSGARV-GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTAS-GSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLE 556          

HSP 5 Score: 63.1586 bits (152), Expect = 4.695e-13
Identity = 36/125 (28.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A +  HL  V+LL+ ++  ++     +    +H A   GH  + KLL+D+       GG+   R      P+H AC+K  +++ ++L++   +I     + L PIH+A + GH  +V LL 
Sbjct:  307 PLHMATQGEHLACVQLLLGRQAPVDDVTL-DYLTPLHVAAHCGHYRVAKLLLDR-------GGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL 423          

HSP 6 Score: 62.3882 bits (150), Expect = 9.797e-13
Identity = 38/130 (29.23%), Postives = 74/130 (56.92%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIE-ACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+++V  L+ ++  I+ A +  N+  A+H A   G  +IV++L++   +I A   + +N   P++ A ++ HLEI K L++   N      +   P+ +A  +GH ++V LL +++   +V+
Sbjct:   48 LHLASKEGHVDVVTELLKREAEIDTATKVGNT--ALHIAALAGQTDIVRVLVEFGANINA---QSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVR 172          

HSP 7 Score: 60.077 bits (144), Expect = 5.926e-12
Identity = 34/128 (26.56%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + KLL+++     A R  N    +H AC++  +++++LL+     I          PIH A   GHL + ++L++   +  A  +    P+H+A   G  E+V  L  +   + V+
Sbjct:  340 PLHVAAHCGHYRVAKLLLDRGGEPNA-RALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLT-PIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVK 465          

HSP 8 Score: 57.7658 bits (138), Expect = 3.832e-11
Identity = 37/123 (30.08%), Postives = 63/123 (51.22%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR----PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+H+A   G++ +  LL+N    ++    +N    +H A ++G+  +VKLL+D+   I+      K R    P+H A   GH ++ ++L+E+   I A   N L P+H+A    H   V LL 
Sbjct:  208 PLHIAAHYGNMNVATLLLNLGAAVDFTA-RNGITPLHVAAKRGNNNMVKLLLDRGAKIDT-----KTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLL 324          

HSP 9 Score: 56.225 bits (134), Expect = 1.618e-10
Identity = 30/119 (25.21%), Postives = 57/119 (47.90%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + GH ++V LL+ +  +I     +    A+H A ++  + + ++L     D+     K    P+H A   G++++   L+  +  + A   N   P+H A  +GH  +V  L 
Sbjct:  639 PLHLAAQEGHADMVALLLGRHASISLAT-KGGLTAMHLAAQEDKVNVAEILTKHKADVNTTT-KLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755          

HSP 10 Score: 55.8398 bits (133), Expect = 1.715e-10
Identity = 37/156 (23.72%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------------------QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLEIVK L++   N ++   ++    +  A +QGH ++V LL++                            QH     +  K    P+H A   G++ +A +L+     ++    N + P+H+A  RG+  +V LL D    I+ +
Sbjct:  113 PLYMAAQENHLEIVKFLLDNGAN-QSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTK 267          

HSP 11 Score: 44.2838 bits (103), Expect = 1.930e-6
Identity = 28/126 (22.22%), Postives = 60/126 (47.62%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID--QHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A +        LL+  + N +    ++    +H A   G++ +  LL++     D  A  G     P+H A ++G+  + K+L+++   I+    + L P+H A   GH +++ +L + +  I
Sbjct:  176 LHIAARKDDTNAAALLLQHEHNADVTS-KSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGI---TPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPI 297          
BLAST of SMED30009057 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 44.2838 bits (103), Expect = 9.249e-7
Identity = 34/124 (27.42%), Postives = 54/124 (43.55%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERA-IHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGIN-----NLRPIHLACWRGHAEIVTLL 365
            P H+AC  G+LE+VK L ++    +  +  N     +H A  +   E+ + LI+    +  I  K+   P+H A   G L+    LIE  C +    +N        P+  A   GH +   LL
Sbjct:   75 PFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVR-IKDKFNQIPLHRAASVGSLK----LIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLL 193          
BLAST of SMED30009057 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 44.669 bits (104), Expect = 1.065e-6
Identity = 34/142 (23.94%), Postives = 61/142 (42.96%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLL--INKKCNIEACRFQNSER--------------AIHFACE-QGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+++ACK  + +  KLL  I  K N +  + ++S+                +H   +  G  ++++LLI    D   I G  K  PI +A   GH E+   L++    ++    N   P+  A W  H +++  L     N+
Sbjct:  510 PLNVACKFNNHDAAKLLLEIRSKQNADNAKNKSSQHLCQPLFKKNSTGLCTLHIVAKIGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQRPLNL 651          
BLAST of SMED30009057 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 43.1282 bits (100), Expect = 2.934e-6
Identity = 29/91 (31.87%), Postives = 46/91 (50.55%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCD-----IEAIGGKWKNRPIHFACEKGHLEIAKVLIE 272
            + +AC  G  ++VK +I +       +      A+H A  QGH+EIV+LLI+   D     IE  G    + P+  A   GHL++ K L++
Sbjct:  334 LQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFG----DTPLIDASANGHLDVVKYLLK 420          
BLAST of SMED30009057 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 41.9726 bits (97), Expect = 7.312e-6
Identity = 27/88 (30.68%), Postives = 42/88 (47.73%), Query Frame = 3
Query:   18 HLACKNGHLEIVKLLINKKCNIEACRFQNSERAI---HFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIE 272
            H AC+ G L  VK +I+ K  +E     +S   I   H+A     L +V  L+ Q  D+ A  G     P+H+A   G++ I   L++
Sbjct:   78 HTACQRGDLATVKEMIHGKL-LEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLK 164          
BLAST of SMED30009057 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672])

HSP 1 Score: 41.9726 bits (97), Expect = 7.496e-6
Identity = 37/128 (28.91%), Postives = 61/128 (47.66%), Query Frame = 3
Query:   36 GHLEIVKLLINKK----CNIEACRFQNSERAIHFACEQGHLEIVKLLI----DQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCN-IEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            G+L IVK L+ +      NI++   +N   ++H+A   G   I   LI    D+H  I+   G   N  +H A  KGH +   +L+++    I   G N   PIH+AC   + + ++LL     ++ V
Sbjct:   16 GNLLIVKRLLRRNPDLLTNIDS---ENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKG---NTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWV 137          
BLAST of SMED30009057 vs. Ensembl Nematostella
Match: EDO28178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T774])

HSP 1 Score: 71.633 bits (174), Expect = 2.298e-16
Identity = 42/120 (35.00%), Postives = 67/120 (55.83%), Query Frame = 3
Query:   36 GHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEK--KCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            GH+++  +L++    I      N +  + FAC +GH ++V+LL+ +  +IE    K    P+ FA   GH ++A  L+E+  K NI + G NN  P+  ACW+GH ++V LL     NIE
Sbjct:   64 GHVKVATMLLDHGAEINVESDSNKDSPLTFACWKGHCDVVELLLARSANIEH-RTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPS-GSNNDIPLTSACWKGHHDVVKLLLKYTSNIE 181          

HSP 2 Score: 59.3066 bits (142), Expect = 8.666e-12
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLA 329
            P+  AC  GH ++V+LL+ +  NIE  R +     + FA   GH ++   L++Q   +    G   + P+  AC KGH ++ K+L++   NIE    +   P+ LA
Sbjct:   90 PLTFACWKGHCDVVELLLARSANIEH-RTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACWKGHHDVVKLLLKYTSNIEHRTKDGCTPLMLA 194          
BLAST of SMED30009057 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35])

HSP 1 Score: 57.3806 bits (137), Expect = 1.780e-11
Identity = 35/123 (28.46%), Postives = 66/123 (53.66%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLI--NKKCNIEACRFQNSERAIHFACEQGHLE-IVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTL 362
            PIH A  NGH E V++L+      N +  +   +   +H+A   GHL+  V++L+++  D+EA+   +++  R IH A + G +++   L++   +IE+      L  +H+A  +G   + TL
Sbjct:   33 PIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDLEAV---YRDGWRLIHVASDNGRVKVLVTLLDAGVDIESKTYERRLTAVHVAARQGQVTMTTL 152          
BLAST of SMED30009057 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 58.5362 bits (140), Expect = 4.153e-11
Identity = 38/129 (29.46%), Postives = 59/129 (45.74%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGK-WKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PIH A K G +  V  L+ +   I       S   +H A   GH  +VK L+ +   I+A     W   P+  A  +G +E+A +L+++   ++        P+HLA   GH E+V LL     NI + 
Sbjct:  286 PIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWT--PLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLT 412          

HSP 2 Score: 58.151 bits (139), Expect = 4.801e-11
Identity = 34/120 (28.33%), Postives = 60/120 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+HLA  +GH  +VK L+ +   I+A R       +  A  +G +E+  +L+D+   ++    K K  P+H A  +GH+E+ K+L+ +K NI          + +A    H E+   + D
Sbjct:  320 PLHLAALHGHAAVVKELLYRGGAIDA-RNATLWTPLDCAAARGMVEVANILLDEDAPVDPTD-KAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIID 437          

HSP 3 Score: 46.2098 bits (108), Expect = 8.026e-7
Identity = 30/106 (28.30%), Postives = 52/106 (49.06%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG------------KWKNRPIHFACEKGHLEIAKVLIEKKCNIEA 293
            P+H+AC +G + + K L+    ++ +   +     +HFAC +G+L + KLL       E  GG            + +   +H A E GH ++AK+ +EK  N+ A
Sbjct:    7 PLHVACSHGCIPVAKCLVEAGADLRSLD-EEQMTPLHFACMEGNLGVAKLLF---VAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNA 108          

HSP 4 Score: 43.1282 bits (100), Expect = 8.407e-6
Identity = 19/58 (32.76%), Postives = 31/58 (53.45%), Query Frame = 3
Query:  201 KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            K +  P+H AC  G + +AK L+E   ++ +     + P+H AC  G+  +  LLF A
Sbjct:    2 KTQMTPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVA 59          
BLAST of SMED30009057 vs. Ensembl Nematostella
Match: EDO34823 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNB2])

HSP 1 Score: 58.151 bits (139), Expect = 5.626e-11
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = 3
Query:    9 HPIHLACKNGHLEIVKLL-INKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDI---EAIGGKWKNRPIHFACEKGHLEIAKVLIE-KKCNIEAWGINNLRPIHLACWRGH 344
            HPIH A +NGHL+ +  L I+ + ++   R +      H+A  QGHL ++K L +  CD+   +++GG     P+H A EKG L   + L+E    ++ +   +N  PI LA   GH
Sbjct:   67 HPIHAAAQNGHLDCLGYLAIHGRVSMTE-RDRTGATPAHYAAGQGHLSVLKWL-NVRCDVKIKDSLGGT----PLHDAAEKGQLHCLRFLVEVVHLDVRSKDSSNTSPIDLAERYGH 177          
BLAST of SMED30009057 vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11])

HSP 1 Score: 53.1434 bits (126), Expect = 1.782e-9
Identity = 35/115 (30.43%), Postives = 56/115 (48.70%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHA 347
            LS P+H+ACK G +E+ +LL+ K   ++  +    E  +H A   G  + +K L+ +   I+      +  P   A   G  E A +L+EK  N ++       P+HLAC  G A
Sbjct:   36 LSTPLHVACKKGSMEVGRLLVEKGAALDK-KNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRT-PFLSAVAAGRTENAMLLLEKDENQKS-------PLHLACAAGRA 141          
BLAST of SMED30009057 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 80.4925 bits (197), Expect = 2.219e-18
Identity = 42/124 (33.87%), Postives = 67/124 (54.03%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  HL ++ LL+    +IEA   ++    +H A   GHL IVK+L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL +   N
Sbjct:  312 PLHIACKKNHLRVMDLLLKHSASIEAVT-ESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVET-PLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKAN 433          

HSP 2 Score: 78.5666 bits (192), Expect = 1.088e-17
Identity = 40/126 (31.75%), Postives = 70/126 (55.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH  I+++L++    I+A + +N    IH A +  H++ VK L+  + +I+ I       P+H A   GH  +AKVL++K     +  +N   P+H+AC + H  ++ LL   + +IE
Sbjct:  213 PLHCAARNGHFRIIEILLDNGAPIQA-KTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIE 336          

HSP 3 Score: 75.485 bits (184), Expect = 1.548e-16
Identity = 41/120 (34.17%), Postives = 61/120 (50.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A   GHL IVK+L+ K  +  A   +  E  +H A   GH E+ + L+     ++A   K    P+H A   GH E+ K+L+E K N  +       P+H+A   GH + V LL D
Sbjct:  345 PLHVASFMGHLNIVKILLQKGASPSASNVK-VETPLHMASRSGHFEVAEFLLQNAAPVDA-KAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLD 462          

HSP 4 Score: 73.559 bits (179), Expect = 5.951e-16
Identity = 41/121 (33.88%), Postives = 68/121 (56.20%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR---PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL+  K N  +         +H A  +GH++ V+LL+D    +EA   K   +   P+H A + G +++A++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  411 PLHCAARMGHKELVKLLLEHKANPNSTT-TAGHSPLHIAAREGHVQTVRLLLD----MEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLL 526          

HSP 5 Score: 64.6994 bits (156), Expect = 7.713e-13
Identity = 30/124 (24.19%), Postives = 64/124 (51.61%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+A ++GH E+ + L+     ++A + ++ +  +H A   GH E+VKLL++   +  +      + P+H A  +GH++  ++L++ +            P+H+A   G  ++  LL +   N
Sbjct:  378 PLHMASRSGHFEVAEFLLQNAAPVDA-KAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTA-GHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGAN 499          

HSP 6 Score: 62.7734 bits (151), Expect = 3.594e-12
Identity = 33/124 (26.61%), Postives = 63/124 (50.81%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            +H+A K   +E+   L+    +  A   Q     +H A ++G  +IV LLI +  ++  +G K    P+H   ++GH+ IA +L+++  ++ A       P+H+AC  G+ ++V  L     N+
Sbjct:  544 LHIAAKQNQVEVANSLLQHGASANAESLQGVT-PLHLASQEGRPDIVSLLISKQANVN-LGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANV 665          

HSP 7 Score: 58.9214 bits (141), Expect = 8.838e-11
Identity = 32/133 (24.06%), Postives = 65/133 (48.87%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEA---------------WGINNLRPIHLACWRGHAEIVTLL 365
            P+HLA + G  +IV LLI+K+ N+     ++    +H   ++GH+ I  +L+ Q   + A   +    P+H AC  G++++ K L++++ N+ +                   +  P+H   W G+  ++ +L
Sbjct:  576 PLHLASQEGRPDIVSLLISKQANVNLGN-KSGLTPLHLVAQEGHVGIADILVKQGASVYA-ATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKTRVEYIQCHYKDHYCEYKHTTPLHTHLWLGYISVIDVL 706          

HSP 8 Score: 58.5362 bits (140), Expect = 1.181e-10
Identity = 32/127 (25.20%), Postives = 64/127 (50.39%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G +++ +LL+ +  N  A   +N    +H A    +L++V LL+ +     +   +     +H A ++  +E+A  L++   +  A  +  + P+HLA   G  +IV+LL     N+ +
Sbjct:  477 PLHVASKYGKVDVAELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVNLLVSKGGSPHS-AARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNL 601          

HSP 9 Score: 54.299 bits (129), Expect = 3.582e-9
Identity = 39/145 (26.90%), Postives = 65/145 (44.83%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-----------------PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI       + G K K R                 P+H A    +L +A++L+ +  N E      L P+H A   GH  I+ +L D    I+ +
Sbjct:  109 PLYMAAQENHLEVVKFLLENGAN-QSIPTEDGFTPLAVALQQGHENVVALLI-------SYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDE------LTPLHCAARNGHFRIIEILLDNGAPIQAK 239          

HSP 10 Score: 53.9138 bits (128), Expect = 5.072e-9
Identity = 33/117 (28.21%), Postives = 59/117 (50.43%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            +HLA K GH+++V  L++    +E    +    A+H A   G  ++V  L++   ++ A   K    P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V LL
Sbjct:   44 LHLASKEGHVKMVLELLHNGIVLETTT-KKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFT-PLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALL 158          

HSP 11 Score: 45.8246 bits (107), Expect = 3.309e-6
Identity = 30/120 (25.00%), Postives = 53/120 (44.17%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A ++G+L+     I    +I     QN   A+H A ++GH+++V  L+     +E    K  N  +H A   G  ++   L+    N+ A       P+++A    H E+V  L +   N
Sbjct:   14 AARSGNLDKALEHIKNGIDINTAN-QNGLNALHLASKEGHVKMVLELLHNGIVLETTTKK-GNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGAN 131          
BLAST of SMED30009057 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 76.6406 bits (187), Expect = 5.129e-17
Identity = 41/124 (33.06%), Postives = 65/124 (52.42%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            P+H+ACK  H+  + LL+    ++EA   ++    +H A   GHL IVK L+ +     A   K +  P+H A   GH E+A+ L++    ++A   ++  P+H A   GH E+V LL D   N
Sbjct:  372 PLHIACKKNHMRSMDLLLKHSASLEAVT-ESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVET-PLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKAN 493          

HSP 2 Score: 75.8702 bits (185), Expect = 1.092e-16
Identity = 39/126 (30.95%), Postives = 69/126 (54.76%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A +NGH+ I+++L+     I+A + +N    IH A +  H++ V+ L+  + +I+ I       P+H A   GH  +AKVL++K     A  +N   P+H+AC + H   + LL   + ++E
Sbjct:  273 PLHCAARNGHVRIIEILLEHGAPIQA-KTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLT-PLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLE 396          

HSP 3 Score: 72.4034 bits (176), Expect = 1.867e-15
Identity = 36/119 (30.25%), Postives = 66/119 (55.46%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLF 368
            P+HLA + G  ++V +LI+K+ N+     +N    +H   ++GH+ I   L+ Q   + A   +    P+H AC  G++++ K L++++ ++ A       P+H A  +GH +IVTLL 
Sbjct:  636 PLHLASQEGRPDMVAMLISKQANVNLGN-KNGLTPLHLVAQEGHVGIADTLVKQGASVYA-ASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLL 752          

HSP 4 Score: 70.4774 bits (171), Expect = 6.906e-15
Identity = 37/118 (31.36%), Postives = 66/118 (55.93%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H A + GH E+VKLL++ K N ++         +H A  +GH+  +++L+D       +  K    P+H A + G +++A++L+E+  N  A G N L P+H+A    + ++V LL
Sbjct:  471 PLHCAARMGHKELVKLLLDHKANPDSAT-TAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFT-PLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 586          

HSP 5 Score: 69.707 bits (169), Expect = 1.321e-14
Identity = 38/121 (31.40%), Postives = 61/121 (50.41%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDA 374
            P+H+A   GHL IVK L+ +  +  A   +  E  +H A   GH E+ + L+     ++A   K    P+H A   GH E+ K+L++ K N ++       P+H+A   GH   + +L DA
Sbjct:  405 PLHVAAFMGHLNIVKNLLQRGASPNASNVK-VETPLHMASRAGHCEVAQFLLQNAAQVDA-RAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDA 523          

HSP 6 Score: 65.855 bits (159), Expect = 2.846e-13
Identity = 36/128 (28.12%), Postives = 62/128 (48.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A   GH  + K+L++K     A R  N    +H AC++ H+  + LL+     +EA+       P+H A   GHL I K L+++  +  A  +    P+H+A   GH E+   L      ++ +
Sbjct:  339 PLHVAAHCGHHRMAKVLLDKGAKANA-RALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLT-PLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDAR 464          

HSP 7 Score: 62.003 bits (149), Expect = 6.241e-12
Identity = 32/127 (25.20%), Postives = 65/127 (51.18%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            P+H+A K G +++ +LL+ +  N  A   +N    +H A    +L++VKLL+ +     +   +    P+H A ++  +E+A VL++   +     +  + P+HLA   G  ++V +L     N+ +
Sbjct:  537 PLHVASKYGKVDVAELLLERGANPNAAG-KNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTA-RNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNL 661          

HSP 8 Score: 61.2326 bits (147), Expect = 1.434e-11
Identity = 30/128 (23.44%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+H+A K   +E+  +L+    +   C        +H A ++G  ++V +LI +  ++  +G K    P+H   ++GH+ IA  L+++  ++ A       P+H+AC  G+ ++V  L     ++  +
Sbjct:  603 PLHIAAKQNQIEVASVLLQNGAS-PNCESLQGITPLHLASQEGRPDMVAMLISKQANVN-LGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAK 728          

HSP 9 Score: 60.4622 bits (145), Expect = 2.174e-11
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC--DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+H+A  + +L++VKLL++K  +  +   +N    +H A +Q  +E+  +L+      + E++ G     P+H A ++G  ++  +LI K+ N+     N L P+HL    GH  I   L
Sbjct:  570 PLHVAVHHNNLDVVKLLVSKGGSAHSTA-RNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGI---TPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTL 685          

HSP 10 Score: 56.9954 bits (136), Expect = 4.487e-10
Identity = 34/119 (28.57%), Postives = 62/119 (52.10%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +HLA K GH+++V  L++    +EA   +    A+H A   G  ++V  L++   ++ A   K  + P++ A ++ HLE+ K L+E   N      +   P+ +A  +GH  +V LL +
Sbjct:   47 LHLASKEGHVKMVLELLHSGIELEATT-KKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFS-PLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLIN 163          

HSP 11 Score: 54.299 bits (129), Expect = 3.253e-9
Identity = 37/156 (23.72%), Postives = 69/156 (44.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLID----------------QHCDIEAIGGKWKN------------RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HLE+VK L+    N ++   ++    +  A +QGH  +V LLI+                ++ D        +N             P+H A    ++ +A++L+ +  N+     N + P+H+A  RG+  +V LL D    I+ Q
Sbjct:  112 PLYMAAQENHLEVVKFLLENGAN-QSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQ 266          

HSP 12 Score: 51.2174 bits (121), Expect = 3.890e-8
Identity = 29/118 (24.58%), Postives = 58/118 (49.15%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+HL  + GH+ I   L+ +  ++ A   +     +H AC  G++++VK L+ Q   + A   +    P+H A ++GH +I  +L++          N   P+ +A   G+  ++ +L
Sbjct:  669 PLHLVAQEGHVGIADTLVKQGASVYAAS-RMGYTPLHVACHYGNIKMVKFLLQQQAHVNA-KTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVL 784          

HSP 13 Score: 48.1358 bits (113), Expect = 4.173e-7
Identity = 31/120 (25.83%), Postives = 54/120 (45.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCN 383
            A ++G+L+     I    NI     QN    +H A ++GH+++V  L+    ++EA   K  N  +H A   G  ++   L+    N+ A       P+++A    H E+V  L +   N
Sbjct:   17 AARSGNLDKALDHIKNGININIAN-QNGLNGLHLASKEGHVKMVLELLHSGIELEATTKK-GNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGAN 134          

HSP 14 Score: 46.9802 bits (110), Expect = 1.159e-6
Identity = 26/127 (20.47%), Postives = 61/127 (48.03%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +H+A +N       +L+    N +    +     +H A    ++ + +LL+++  ++     K    P+H A  +G++ + ++L+++   I+A   + L P+H A   GH  I+ +L +    I+ +
Sbjct:  175 LHIAARNDDTRTAAVLLQNDPNPDVLS-KTGFTPLHIAAHYENMSVAQLLLNRGANVN-FTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAK 299          
BLAST of SMED30009057 vs. Ensembl Medaka
Match: ripk4 (receptor interacting serine/threonine kinase 4 [Source:NCBI gene;Acc:101174941])

HSP 1 Score: 75.8702 bits (185), Expect = 8.882e-17
Identity = 44/151 (29.14%), Postives = 78/151 (51.66%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQH-CDIE-------------AIGGKWK-------------------NRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P H+AC++G   ++++L+++  +++  + +++  A+HFA  QGHL IVKLL+ Q   D++             +  G+++                   N P+H A E GH   +++LI+ K ++ A     L P+HLAC RGH   V +L
Sbjct:  545 PAHVACQHGQENVIRVLLSRGADVQ-IKGKDNWTALHFAAWQGHLGIVKLLVKQAGADVDGQTSDGRTSLHLASQRGQYRVARILIELGASVHLTSVGLNTPLHVAAETGHTSTSRLLIKHKADMHAQNTQGLTPLHLACQRGHLATVKML 694          

HSP 2 Score: 68.5514 bits (166), Expect = 3.677e-14
Identity = 35/120 (29.17%), Postives = 64/120 (53.33%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            S P+HLA +     + +LL+ ++      + ++   A+H+A + G   I +LL+D+   I    G+ +  P H AC+ G   + +VL+ +  +++  G +N   +H A W+GH  IV LL
Sbjct:  476 STPLHLATEKHQKPLAELLLGRRSMNVNAKDEDQFTALHWAAQNGDEAIARLLLDRGAAINETDGQGRT-PAHVACQHGQENVIRVLLSRGADVQIKGKDNWTALHFAAWQGHLGIVKLL 594          

HSP 3 Score: 63.929 bits (154), Expect = 1.590e-12
Identity = 42/127 (33.07%), Postives = 68/127 (53.54%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSE--RAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINN---LRPIHLACWRGHAEIVTLLF 368
            L+ P+H+A + GH    +LLI  K ++ A   QN++    +H AC++GHL  VK+LI +  D  +   +    P H A   GH E+ K L+   C  +A  + +   L P+HLA   GH+ ++ +L 
Sbjct:  642 LNTPLHVAAETGHTSTSRLLIKHKADMHA---QNTQGLTPLHLACQRGHLATVKMLIAEGAD-PSRPSQTLCTPCHLAAGSGHCEVLKELL-LHCP-DAGSVTDEEGLSPLHLAVGAGHSNVIKMLL 762          

HSP 4 Score: 48.521 bits (114), Expect = 3.895e-7
Identity = 30/90 (33.33%), Postives = 48/90 (53.33%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHC-DIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKK 278
            P+HLAC+ GHL  VK+LI +  +  +   Q      H A   GH E++K L+  HC D  ++  +    P+H A   GH  + K+L+ ++
Sbjct:  678 PLHLACQRGHLATVKMLIAEGAD-PSRPSQTLCTPCHLAAGSGHCEVLKELL-LHCPDAGSVTDEEGLSPLHLAVGAGHSNVIKMLLPQE 765          
BLAST of SMED30009057 vs. Ensembl Medaka
Match: ENSORLT00000040233.1 (pep primary_assembly:ASM223467v1:19:16412534:16434057:1 gene:ENSORLG00000021910.1 transcript:ENSORLT00000040233.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 1.680e-15
Identity = 36/126 (28.57%), Postives = 71/126 (56.35%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+H A ++GH ++V++L+N+   I + + +N    +H A +  HL  V+LL+     ++ +   +    +H A   GH ++AKV+++KK N  A  +N   P+H+AC +   +++ LL     +I+
Sbjct:  280 PLHCAARSGHEQVVEMLLNRGAPILS-KTKNGLSPLHMATQGDHLNCVQLLLHHDVPVDDVTNDYLT-ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASIQ 403          

HSP 2 Score: 61.2326 bits (147), Expect = 1.156e-11
Identity = 35/122 (28.69%), Postives = 69/122 (56.56%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCD--IEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            P+H+A +  HL  V+LL++    ++     +   A+H A   GH ++ K+++D+  +   +A+ G     P+H AC+K  +++ ++L++   +I+A   + L PIH+A + GH  IV  L +
Sbjct:  313 PLHMATQGDHLNCVQLLLHHDVPVDDVT-NDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNG---FTPLHIACKKNRVKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVHQLIN 430          

HSP 3 Score: 61.2326 bits (147), Expect = 1.323e-11
Identity = 36/116 (31.03%), Postives = 58/116 (50.00%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            A + G+LE     +    +I  C  QN   A+H A ++GH+E+V  LI Q  +++A   K  N  +H A   G  E+ K L+    N+ A   N   P+++A    H ++V LL +
Sbjct:   24 AARAGNLEKALDYLQNGVDINICN-QNGLNALHLASKEGHVEVVAELIKQGANVDA-ATKKGNTALHIASLAGQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLE 137          

HSP 4 Score: 60.4622 bits (145), Expect = 2.046e-11
Identity = 36/129 (27.91%), Postives = 71/129 (55.04%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            +HLA K GH+E+V  LI +  N++A   +    A+H A   G  E+VK L+    ++ A   + +N   P++ A ++ HL++ ++L+E   +      +   P+ +A  +GH ++V+LL + +   +V+
Sbjct:   54 LHLASKEGHVEVVAELIKQGANVDAAT-KKGNTALHIASLAGQTEVVKELVSNGANVNA---QSQNGFTPLYMAAQENHLDVVQLLLENGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR 178          

HSP 5 Score: 50.8322 bits (120), Expect = 5.204e-8
Identity = 34/159 (21.38%), Postives = 74/159 (46.54%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLI-------------------------------DQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            P+++A +  HL++V+LL+    + ++   ++    +  A +QGH ++V LL+                               D + D+E+  G     P+H A   G++ +A +L+ +   ++    N++ P+H+A  RG++ +V LL +    I+ +
Sbjct:  119 PLYMAAQENHLDVVQLLLENGSS-QSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSG---FTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVRLLLERGSKIDAR 273          

HSP 6 Score: 46.595 bits (109), Expect = 1.293e-6
Identity = 29/121 (23.97%), Postives = 62/121 (51.24%), Query Frame = 3
Query:   15 IHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKN--RPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFD 371
            +H+A +    +   LL+    N +    ++    +H A   G++ +  LL+++     A+  K +N   P+H A ++G+  + ++L+E+   I+A   + L P+H A   GH ++V +L +
Sbjct:  182 LHIAARKDDTKAAALLLQNDHNADV-ESKSGFTPLHIAAHYGNINVATLLLNRGA---AVDFKARNDITPLHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLN 298          
BLAST of SMED30009057 vs. Ensembl Medaka
Match: ankhd1 (ankyrin repeat and KH domain-containing protein 1 [Source:NCBI gene;Acc:101167550])

HSP 1 Score: 72.4034 bits (176), Expect = 1.912e-15
Identity = 39/122 (31.97%), Postives = 67/122 (54.92%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            LAC  GH E+V +L+ +  NIE  R +     +  A   GH+ +V++L+D+  DIEA   + K+ P+  AC  G  E+ ++L+ +  N E   +++  P+ LA   G+  I+ +L +A   I
Sbjct: 1117 LACAGGHEELVSVLLARGANIEH-RDKKGFTPLILAATAGHVGVVEVLLDKCGDIEAQSERTKDTPLSLACSGGRQEVVELLLLRGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEI 1237          

HSP 2 Score: 70.4774 bits (171), Expect = 8.833e-15
Identity = 40/125 (32.00%), Postives = 63/125 (50.40%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LAC  GHL++V+ L+    + E  +      A+  AC  GH+E+ +LL+D    +      +++ P+  A   GH+E+A +LIE+  N+E        P+  A   GH E+V LL     NI  Q
Sbjct:  369 LACYKGHLDMVRFLLEAGADQEH-KTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFES-PLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQ 491          

HSP 3 Score: 70.0922 bits (170), Expect = 1.031e-14
Identity = 42/126 (33.33%), Postives = 63/126 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+  A + GH E+V LL+ +  NI A   +  E A+  AC  G LE+   LI    DIE +G    + P+  A ++GHLE+ K L+    N+ A        +  AC  GH ++  +L  A  N+E
Sbjct:  465 PLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADIE-LGC---STPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADVLLQAGANLE 586          

HSP 4 Score: 66.6254 bits (161), Expect = 1.997e-13
Identity = 41/128 (32.03%), Postives = 68/128 (53.12%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEA-IGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAW-GINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+ LAC  G  E+V+LL+ +  N E  R  +    +  A   G++ I+K+L++   +I +  G K    P+  A   GH+   K+L++   +I A    N    + LAC++G AE+V+LL D   N+E
Sbjct: 1181 PLSLACSGGRQEVVELLLLRGANKEH-RNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVE 1307          

HSP 5 Score: 64.3142 bits (155), Expect = 1.269e-12
Identity = 37/130 (28.46%), Postives = 64/130 (49.23%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHF----ACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+  A   GH+E+ ++L+     I     +  E A+  AC +GHL++V+ L++   D E      K   +H     AC  GH+E+A++L++    +     +   P+ LA   GH E+  LL +   N+E
Sbjct:  332 PLMEAASAGHVEVARVLLEYGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEH-----KTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLE 456          

HSP 6 Score: 63.5438 bits (153), Expect = 2.312e-12
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 3
Query:    6 SHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIE--AIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPI-HLACWRGHAEIVTLLF 368
            S P+  A + GHLE+VK L+    N+ A      + A+ +ACE GH ++  +L+    ++E  + GG+    P+  A   GHL   + LI K  N+     NN   +  LAC  GH  +V LL 
Sbjct:  527 STPLMEAAQEGHLELVKYLLAAGANVHATT-ATGDTALTYACENGHTDVADVLLQAGANLEHESEGGR---TPLMKAARAGHLCTVQFLISKGANVNRTTANNDHTVVSLACAGGHLAVVELLL 646          

HSP 7 Score: 63.1586 bits (152), Expect = 2.475e-12
Identity = 34/123 (27.64%), Postives = 61/123 (49.59%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEV 392
            AC  G +++VK+L+ +  NIE    +N    +  A   GH+E+ ++L++    I     ++K   +  AC KGHL++ + L+E   + E         +  AC  GH E+  LL D+   + +
Sbjct:  303 ACAGGFVDVVKVLLKEGANIED-HNENGHTPLMEAASAGHVEVARVLLEYGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNM 424          

HSP 8 Score: 63.1586 bits (152), Expect = 2.834e-12
Identity = 39/128 (30.47%), Postives = 64/128 (50.00%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIH---FACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNI 386
            P+ LA   G++ I+K+L+N    I +     S+  I     A   GH+  VKLL+D   DI A     +N  +  AC +G  E+  +L+++K N+E      L P+  A   G+AE+  +L D   ++
Sbjct: 1214 PLSLAASGGYVNIIKILLNAGAEINS--RTGSKLGISPLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLMEAASGGYAEVGRVLLDKGADV 1339          

HSP 9 Score: 60.8474 bits (146), Expect = 1.764e-11
Identity = 44/160 (27.50%), Postives = 71/160 (44.38%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIE-------------AIGG---------------------KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            P+ LA  NGH+  VKLL++   +I A    N   A+  AC QG  E+V LL+D+  ++E             A GG                       ++  +  A +KGH +  ++LI +  +I+        P+ LA   GH E+V LL  A+ +++
Sbjct: 1249 PLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLINRCAHIDVRNKKGNTPLWLAANGGHFEVVQLLVHASADVD 1408          

HSP 10 Score: 59.3066 bits (142), Expect = 7.104e-11
Identity = 35/125 (28.00%), Postives = 60/125 (48.00%), Query Frame = 3
Query:   21 LACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LAC  G+ E+ ++L+    N+E    +     +  A   G+++IVKLL+    D+ A      N  + +AC  G +++ KVL+++  NIE    N   P+  A   GH E+  +L +    I   
Sbjct:  235 LACSAGYYELAQVLLAMHANVEDRGIKGDITPLMAAASGGYVDIVKLLLVHGADVNAQSST-GNTALTYACAGGFVDVVKVLLKEGANIEDHNENGHTPLMEAASAGHVEVARVLLEYGAGINTH 358          

HSP 11 Score: 55.8398 bits (133), Expect = 9.853e-10
Identity = 31/119 (26.05%), Postives = 64/119 (53.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNS-ERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P+  A   G+ E+ ++L++K  ++ A    +S + A+  A ++GH +  +LLI++   I+ +  K  N P+  A   GH E+ ++L+    +++A     + P+  A  +GH ++V  L
Sbjct: 1316 PLMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLINRCAHID-VRNKKGNTPLWLAANGGHFEVVQLLVHASADVDAADNRKITPLMAAFRKGHVKVVQYL 1433          

HSP 12 Score: 51.6026 bits (122), Expect = 2.923e-8
Identity = 30/122 (24.59%), Postives = 59/122 (48.36%), Query Frame = 3
Query:   24 ACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            AC +G +  V+ L+++  ++     +  E  +  AC  G+ E+ ++L+  H ++E  G K    P+  A   G+++I K+L+    ++ A        +  AC  G  ++V +L     NIE
Sbjct:  203 ACSDGDVNAVRKLLDEGRSVNE-HTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGIKGDITPLMAAASGGYVDIVKLLLVHGADVNAQSSTGNTALTYACAGGFVDVVKVLLKEGANIE 323          

HSP 13 Score: 49.2914 bits (116), Expect = 1.742e-7
Identity = 35/105 (33.33%), Postives = 53/105 (50.48%), Query Frame = 3
Query:   78 NIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNL-RPIHLACWRGHAEIVTLLFDANCNIE 389
            +I+A    N + A+  AC  GH E+V +L+ +  +IE    K    P+  A   GH+ + +VL++K  +IEA        P+ LAC  G  E+V LL     N E
Sbjct: 1102 DIDAHTESNHDTALTLACAGGHEELVSVLLARGANIEHRDKK-GFTPLILAATAGHVGVVEVLLDKCGDIEAQSERTKDTPLSLACSGGRQEVVELLLLRGANKE 1205          
BLAST of SMED30009057 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 235.728 bits (600), Expect = 1.283e-73
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKG---------------------------------HLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG+WKNRPIHFACE+G                                 HLEIAKVLIEKKCNIEAW INNLRPIHLAC  GHAEIVTLLFDA CNIEVQ
Sbjct:  595 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQ 758          

HSP 2 Score: 153.295 bits (386), Expect = 6.112e-44
Identity = 78/126 (61.90%), Postives = 98/126 (77.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GH +IVKLLI++KC+IE+    N +R IHFACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N RPIH+AC  GH +IV LL D +C+IE
Sbjct:  332 PIYVACKQGHFDIVKLLIDQKCDIESADNSN-QRPIHFACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIE 455          

HSP 3 Score: 146.747 bits (369), Expect = 1.047e-41
Identity = 76/126 (60.32%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+ GH +IVKLLI++KC+IE+    N +R IH ACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N  PIH+AC  GH +IV LL D +C+IE
Sbjct:  200 PIHFACEQGHFDIVKLLIDQKCDIESADNSN-QRPIHVACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIE 323          

HSP 4 Score: 145.976 bits (367), Expect = 1.858e-41
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++R IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC+KGHLEI K+LI + C+IE+    N RPIH+AC +GH E+V LL D +C+IE
Sbjct:  398 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQRPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIE 521          

HSP 5 Score: 143.665 bits (361), Expect = 1.321e-40
Identity = 71/126 (56.35%), Postives = 96/126 (76.19%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++  IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC++GH +I K+LI++KC+IE+   +N RPIH AC +GH +IV LL D  C+IE
Sbjct:  266 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQCPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIE 389          

HSP 6 Score: 143.28 bits (360), Expect = 1.768e-40
Identity = 77/130 (59.23%), Postives = 98/130 (75.38%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            L  PIHLACK+G  +IVKLLI+  C+IEA R   + R IHFACE+G L+IVKLLID  CDIEA+  +  NR PIHFACE+GH +I K+LI++KC+IE+   +N RPIH+AC +GH +IV LL D  C+IE
Sbjct:  131 LVKPIHLACKHGFPDIVKLLIDHNCDIEA-RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKR--NRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIE 257          

HSP 7 Score: 141.739 bits (356), Expect = 6.368e-40
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHLEIVKLLIN+ C+IE+    N +R IH AC QGH E+VKLLIDQHCDIE+       RPI+ AC++GHL+I K+LI++KC+IE+      RPIH+AC + H EIV LL   NC+IE
Sbjct:  464 PIYVACKKGHLEIVKLLINQHCDIESADNCN-QRPIHVACNQGHFEVVKLLIDQHCDIES-ADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIE 587          

HSP 8 Score: 141.354 bits (355), Expect = 7.808e-40
Identity = 75/131 (57.25%), Postives = 94/131 (71.76%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            L  PIH AC NGHLEI+KLLI +KC+I A   +     ++FAC +GHLEIVKLLIDQ+CD EA+  K  ++PIH AC +GHL I K+LI+ KC+IEA    N RPIH AC +GH +IV LL D NC+IE +
Sbjct:  763 LLRPIHFACNNGHLEIMKLLIAQKCDINAVN-KCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVD-KDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEAR 891          

HSP 9 Score: 138.272 bits (347), Expect = 1.217e-38
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC-RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PI LAC+NGHLEIVKLL++ KC+IEA  +   +ER+IHFAC+QGHL +VK L+DQ+C++EA   + K +PIH A  KGH++I ++LI+ KC+IEA   + ++PIHLAC  G  +IV LL D NC+IE +
Sbjct:   33 PIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEAR 160          

HSP 10 Score: 137.887 bits (346), Expect = 1.303e-38
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC++GHLEIVKLLI+QHCDIE+       RPIH AC +GH E+ K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  431 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKKGHLEIVKLLINQHCDIES-ADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIE 554          

HSP 11 Score: 137.502 bits (345), Expect = 1.920e-38
Identity = 77/128 (60.16%), Postives = 94/128 (73.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHL+IVKLLI++KC+IE+      +R IH ACE+ HLEIVKLLI Q+CDIEA    K  + PIH AC+ GHLEI K+LI KKCNIEA    N+ R IH AC +GH EIV LL D +C+IE
Sbjct:  530 PIYVACKQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIE 656          

HSP 12 Score: 125.946 bits (315), Expect = 2.186e-34
Identity = 69/126 (54.76%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+  HLEI K+LI KKCNIEA    N+ R IH AC  GH EIV LL D  C+IE        RPIHFAC  GHLEI K+LI +KC+I A       P++ AC +GH EIV LL D NC+ E
Sbjct:  699 PIHFACEKDHLEIAKVLIEKKCNIEAWDI-NNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTE 823          

HSP 13 Score: 116.701 bits (291), Expect = 3.240e-31
Identity = 63/118 (53.39%), Postives = 79/118 (66.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P++ AC  GHLEIVKLLI++ C+ EA    NS + IH AC QGHL I KLLID  CDIEA+  K   RPIHFAC +GH +I K+LI++ C+IEA      RPI +A  +   +IV LL
Sbjct:  799 PVYFACSKGHLEIVKLLIDQNCDTEAVDKDNS-KPIHQACRQGHLNITKLLIDHKCDIEAVD-KCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 914          

HSP 14 Score: 94.3597 bits (233), Expect = 2.523e-23
Identity = 47/99 (47.47%), Postives = 63/99 (63.64%), Query Frame = 3
Query:   96 FQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
             Q + + I  AC  GHLEIVKLL+   CDIEA+       R IHFAC++GHL + K L+++ CN+EA     ++PIH A  +GH +IV LL D  C+IE
Sbjct:   27 LQKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIE 125          
BLAST of SMED30009057 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 235.343 bits (599), Expect = 1.380e-73
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKG---------------------------------HLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG+WKNRPIHFACE+G                                 HLEIAKVLIEKKCNIEAW INNLRPIHLAC  GHAEIVTLLFDA CNIEVQ
Sbjct:  584 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQ 747          

HSP 2 Score: 152.91 bits (385), Expect = 6.883e-44
Identity = 78/126 (61.90%), Postives = 98/126 (77.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GH +IVKLLI++KC+IE+    N +R IHFACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N RPIH+AC  GH +IV LL D +C+IE
Sbjct:  321 PIYVACKQGHFDIVKLLIDQKCDIESADNSN-QRPIHFACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIE 444          

HSP 3 Score: 146.747 bits (369), Expect = 1.183e-41
Identity = 76/126 (60.32%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+ GH +IVKLLI++KC+IE+    N +R IH ACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N  PIH+AC  GH +IV LL D +C+IE
Sbjct:  189 PIHFACEQGHFDIVKLLIDQKCDIESADNSN-QRPIHVACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIE 312          

HSP 4 Score: 145.976 bits (367), Expect = 2.098e-41
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++R IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC+KGHLEI K+LI + C+IE+    N RPIH+AC +GH E+V LL D +C+IE
Sbjct:  387 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQRPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIE 510          

HSP 5 Score: 143.665 bits (361), Expect = 1.465e-40
Identity = 71/126 (56.35%), Postives = 96/126 (76.19%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++  IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC++GH +I K+LI++KC+IE+   +N RPIH AC +GH +IV LL D  C+IE
Sbjct:  255 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQCPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIE 378          

HSP 6 Score: 143.28 bits (360), Expect = 2.018e-40
Identity = 77/130 (59.23%), Postives = 98/130 (75.38%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            L  PIHLACK+G  +IVKLLI+  C+IEA R   + R IHFACE+G L+IVKLLID  CDIEA+  +  NR PIHFACE+GH +I K+LI++KC+IE+   +N RPIH+AC +GH +IV LL D  C+IE
Sbjct:  120 LVKPIHLACKHGFPDIVKLLIDHNCDIEA-RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKR--NRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIE 246          

HSP 7 Score: 141.739 bits (356), Expect = 7.133e-40
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHLEIVKLLIN+ C+IE+    N +R IH AC QGH E+VKLLIDQHCDIE+       RPI+ AC++GHL+I K+LI++KC+IE+      RPIH+AC + H EIV LL   NC+IE
Sbjct:  453 PIYVACKKGHLEIVKLLINQHCDIESADNCN-QRPIHVACNQGHFEVVKLLIDQHCDIES-ADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIE 576          

HSP 8 Score: 141.354 bits (355), Expect = 8.662e-40
Identity = 75/131 (57.25%), Postives = 94/131 (71.76%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            L  PIH AC NGHLEI+KLLI +KC+I A   +     ++FAC +GHLEIVKLLIDQ+CD EA+  K  ++PIH AC +GHL I K+LI+ KC+IEA    N RPIH AC +GH +IV LL D NC+IE +
Sbjct:  752 LLRPIHFACNNGHLEIMKLLIAQKCDINAVN-KCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVD-KDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEAR 880          

HSP 9 Score: 137.887 bits (346), Expect = 1.364e-38
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC-RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PI LAC+NGHLEIVKLL++ KC+IEA  +   +ER+IHFAC+QGHL +VK L+DQ+C++EA   + K +PIH A  KGH++I ++LI+ KC+IEA   + ++PIHLAC  G  +IV LL D NC+IE +
Sbjct:   22 PIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEAR 149          

HSP 10 Score: 137.887 bits (346), Expect = 1.489e-38
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC++GHLEIVKLLI+QHCDIE+       RPIH AC +GH E+ K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  420 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKKGHLEIVKLLINQHCDIES-ADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIE 543          

HSP 11 Score: 137.502 bits (345), Expect = 2.216e-38
Identity = 77/128 (60.16%), Postives = 94/128 (73.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHL+IVKLLI++KC+IE+      +R IH ACE+ HLEIVKLLI Q+CDIEA    K  + PIH AC+ GHLEI K+LI KKCNIEA    N+ R IH AC +GH EIV LL D +C+IE
Sbjct:  519 PIYVACKQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIE 645          

HSP 12 Score: 125.946 bits (315), Expect = 2.476e-34
Identity = 69/126 (54.76%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+  HLEI K+LI KKCNIEA    N+ R IH AC  GH EIV LL D  C+IE        RPIHFAC  GHLEI K+LI +KC+I A       P++ AC +GH EIV LL D NC+ E
Sbjct:  688 PIHFACEKDHLEIAKVLIEKKCNIEAWDI-NNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTE 812          

HSP 13 Score: 116.701 bits (291), Expect = 3.532e-31
Identity = 63/118 (53.39%), Postives = 79/118 (66.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P++ AC  GHLEIVKLLI++ C+ EA    NS + IH AC QGHL I KLLID  CDIEA+  K   RPIHFAC +GH +I K+LI++ C+IEA      RPI +A  +   +IV LL
Sbjct:  788 PVYFACSKGHLEIVKLLIDQNCDTEAVDKDNS-KPIHQACRQGHLNITKLLIDHKCDIEAVD-KCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 903          

HSP 14 Score: 94.3597 bits (233), Expect = 2.750e-23
Identity = 47/99 (47.47%), Postives = 63/99 (63.64%), Query Frame = 3
Query:   96 FQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
             Q + + I  AC  GHLEIVKLL+   CDIEA+       R IHFAC++GHL + K L+++ CN+EA     ++PIH A  +GH +IV LL D  C+IE
Sbjct:   16 LQKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIE 114          
BLAST of SMED30009057 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 235.343 bits (599), Expect = 1.636e-73
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKG---------------------------------HLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG+WKNRPIHFACE+G                                 HLEIAKVLIEKKCNIEAW INNLRPIHLAC  GHAEIVTLLFDA CNIEVQ
Sbjct:  603 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQ 766          

HSP 2 Score: 180.644 bits (457), Expect = 1.171e-53
Identity = 91/131 (69.47%), Postives = 104/131 (79.39%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNL--RPIHLACWRGHAEIVTLLFDANCNIE 389
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNS+RAIHFACE+GHLEIVKLLIDQHCDIEAI  +   +PI  AC  GHLEI K+L+  KC+IEA    ++  R IH AC +GH  +V  L D NCN+E
Sbjct:   36 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVE 166          

HSP 3 Score: 148.288 bits (373), Expect = 2.858e-42
Identity = 76/126 (60.32%), Postives = 98/126 (77.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++  IH ACE GH +IVKLLIDQHCDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N RPIH+AC  GH +IV LL D +C+IE
Sbjct:  340 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQCPIHVACEHGHFDIVKLLIDQHCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIE 463          

HSP 4 Score: 146.747 bits (369), Expect = 1.263e-41
Identity = 76/126 (60.32%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+ GH +IVKLLI++KC+IE+    N +R IH ACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N  PIH+AC  GH +IV LL D +C+IE
Sbjct:  274 PIHFACEQGHFDIVKLLIDQKCDIESADNSN-QRPIHVACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIE 397          

HSP 5 Score: 145.976 bits (367), Expect = 2.284e-41
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++R IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC+KGHLEI K+LI + C+IE+    N RPIH+AC +GH E+V LL D +C+IE
Sbjct:  406 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQRPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIE 529          

HSP 6 Score: 143.28 bits (360), Expect = 2.173e-40
Identity = 77/130 (59.23%), Postives = 98/130 (75.38%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            L  PIHLACK+G  +IVKLLI+  C+IEA R   + R IHFACE+G L+IVKLLID  CDIEA+  +  NR PIHFACE+GH +I K+LI++KC+IE+   +N RPIH+AC +GH +IV LL D  C+IE
Sbjct:  205 LVKPIHLACKHGFPDIVKLLIDHNCDIEA-RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKR--NRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIE 331          

HSP 7 Score: 141.354 bits (355), Expect = 7.599e-40
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHLEIVKLLIN+ C+IE+    N +R IH AC QGH E+VKLLIDQHCDIE+       RPI+ AC++GHL+I K+LI++KC+IE+      RPIH+AC + H EIV LL   NC+IE
Sbjct:  472 PIYVACKKGHLEIVKLLINQHCDIESADNCN-QRPIHVACNQGHFEVVKLLIDQHCDIES-ADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIE 595          

HSP 8 Score: 141.354 bits (355), Expect = 9.500e-40
Identity = 75/131 (57.25%), Postives = 94/131 (71.76%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            L  PIH AC NGHLEI+KLLI +KC+I A   +     ++FAC +GHLEIVKLLIDQ+CD EA+  K  ++PIH AC +GHL I K+LI+ KC+IEA    N RPIH AC +GH +IV LL D NC+IE +
Sbjct:  771 LLRPIHFACNNGHLEIMKLLIAQKCDINAVN-KCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVD-KDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEAR 899          

HSP 9 Score: 137.887 bits (346), Expect = 1.330e-38
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC-RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PI LAC+NGHLEIVKLL++ KC+IEA  +   +ER+IHFAC+QGHL +VK L+DQ+C++EA   + K +PIH A  KGH++I ++LI+ KC+IEA   + ++PIHLAC  G  +IV LL D NC+IE +
Sbjct:  107 PIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEAR 234          

HSP 10 Score: 137.887 bits (346), Expect = 1.615e-38
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC++GHLEIVKLLI+QHCDIE+       RPIH AC +GH E+ K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  439 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKKGHLEIVKLLINQHCDIES-ADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIE 562          

HSP 11 Score: 137.117 bits (344), Expect = 2.358e-38
Identity = 77/128 (60.16%), Postives = 94/128 (73.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHL+IVKLLI++KC+IE+      +R IH ACE+ HLEIVKLLI Q+CDIEA    K  + PIH AC+ GHLEI K+LI KKCNIEA    N+ R IH AC +GH EIV LL D +C+IE
Sbjct:  538 PIYVACKQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIE 664          

HSP 12 Score: 125.561 bits (314), Expect = 2.605e-34
Identity = 69/126 (54.76%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+  HLEI K+LI KKCNIEA    N+ R IH AC  GH EIV LL D  C+IE        RPIHFAC  GHLEI K+LI +KC+I A       P++ AC +GH EIV LL D NC+ E
Sbjct:  707 PIHFACEKDHLEIAKVLIEKKCNIEAWDI-NNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTE 831          

HSP 13 Score: 116.701 bits (291), Expect = 4.013e-31
Identity = 63/118 (53.39%), Postives = 79/118 (66.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P++ AC  GHLEIVKLLI++ C+ EA    NS + IH AC QGHL I KLLID  CDIEA+  K   RPIHFAC +GH +I K+LI++ C+IEA      RPI +A  +   +IV LL
Sbjct:  807 PVYFACSKGHLEIVKLLIDQNCDTEAVDKDNS-KPIHQACRQGHLNITKLLIDHKCDIEAVD-KCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 922          
BLAST of SMED30009057 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 235.343 bits (599), Expect = 1.794e-73
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKG---------------------------------HLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG+WKNRPIHFACE+G                                 HLEIAKVLIEKKCNIEAW INNLRPIHLAC  GHAEIVTLLFDA CNIEVQ
Sbjct:  602 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQ 765          

HSP 2 Score: 180.644 bits (457), Expect = 1.263e-53
Identity = 91/131 (69.47%), Postives = 104/131 (79.39%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNL--RPIHLACWRGHAEIVTLLFDANCNIE 389
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNS+RAIHFACE+GHLEIVKLLIDQHCDIEAI  +   +PI  AC  GHLEI K+L+  KC+IEA    ++  R IH AC +GH  +V  L D NCN+E
Sbjct:   35 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVE 165          

HSP 3 Score: 148.288 bits (373), Expect = 2.970e-42
Identity = 76/126 (60.32%), Postives = 98/126 (77.78%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++  IH ACE GH +IVKLLIDQHCDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N RPIH+AC  GH +IV LL D +C+IE
Sbjct:  339 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQCPIHVACEHGHFDIVKLLIDQHCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIE 462          

HSP 4 Score: 146.747 bits (369), Expect = 1.313e-41
Identity = 76/126 (60.32%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+ GH +IVKLLI++KC+IE+    N +R IH ACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N  PIH+AC  GH +IV LL D +C+IE
Sbjct:  273 PIHFACEQGHFDIVKLLIDQKCDIESADNSN-QRPIHVACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIE 396          

HSP 5 Score: 145.976 bits (367), Expect = 2.421e-41
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++R IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC+KGHLEI K+LI + C+IE+    N RPIH+AC +GH E+V LL D +C+IE
Sbjct:  405 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQRPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIE 528          

HSP 6 Score: 142.895 bits (359), Expect = 2.236e-40
Identity = 77/130 (59.23%), Postives = 98/130 (75.38%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            L  PIHLACK+G  +IVKLLI+  C+IEA R   + R IHFACE+G L+IVKLLID  CDIEA+  +  NR PIHFACE+GH +I K+LI++KC+IE+   +N RPIH+AC +GH +IV LL D  C+IE
Sbjct:  204 LVKPIHLACKHGFPDIVKLLIDHNCDIEA-RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKR--NRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIE 330          

HSP 7 Score: 141.354 bits (355), Expect = 8.053e-40
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHLEIVKLLIN+ C+IE+    N +R IH AC QGH E+VKLLIDQHCDIE+       RPI+ AC++GHL+I K+LI++KC+IE+      RPIH+AC + H EIV LL   NC+IE
Sbjct:  471 PIYVACKKGHLEIVKLLINQHCDIESADNCN-QRPIHVACNQGHFEVVKLLIDQHCDIES-ADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIE 594          

HSP 8 Score: 141.354 bits (355), Expect = 1.007e-39
Identity = 75/131 (57.25%), Postives = 94/131 (71.76%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            L  PIH AC NGHLEI+KLLI +KC+I A   +     ++FAC +GHLEIVKLLIDQ+CD EA+  K  ++PIH AC +GHL I K+LI+ KC+IEA    N RPIH AC +GH +IV LL D NC+IE +
Sbjct:  770 LLRPIHFACNNGHLEIMKLLIAQKCDINAVN-KCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVD-KDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEAR 898          

HSP 9 Score: 137.887 bits (346), Expect = 1.423e-38
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC-RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PI LAC+NGHLEIVKLL++ KC+IEA  +   +ER+IHFAC+QGHL +VK L+DQ+C++EA   + K +PIH A  KGH++I ++LI+ KC+IEA   + ++PIHLAC  G  +IV LL D NC+IE +
Sbjct:  106 PIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEAR 233          

HSP 10 Score: 137.502 bits (345), Expect = 1.728e-38
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC++GHLEIVKLLI+QHCDIE+       RPIH AC +GH E+ K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  438 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKKGHLEIVKLLINQHCDIES-ADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIE 561          

HSP 11 Score: 137.117 bits (344), Expect = 2.474e-38
Identity = 77/128 (60.16%), Postives = 94/128 (73.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHL+IVKLLI++KC+IE+      +R IH ACE+ HLEIVKLLI Q+CDIEA    K  + PIH AC+ GHLEI K+LI KKCNIEA    N+ R IH AC +GH EIV LL D +C+IE
Sbjct:  537 PIYVACKQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIE 663          

HSP 12 Score: 125.561 bits (314), Expect = 2.787e-34
Identity = 69/126 (54.76%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+  HLEI K+LI KKCNIEA    N+ R IH AC  GH EIV LL D  C+IE        RPIHFAC  GHLEI K+LI +KC+I A       P++ AC +GH EIV LL D NC+ E
Sbjct:  706 PIHFACEKDHLEIAKVLIEKKCNIEAWDI-NNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTE 830          

HSP 13 Score: 116.701 bits (291), Expect = 4.295e-31
Identity = 63/118 (53.39%), Postives = 79/118 (66.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P++ AC  GHLEIVKLLI++ C+ EA    NS + IH AC QGHL I KLLID  CDIEA+  K   RPIHFAC +GH +I K+LI++ C+IEA      RPI +A  +   +IV LL
Sbjct:  806 PVYFACSKGHLEIVKLLIDQNCDTEAVDKDNS-KPIHQACRQGHLNITKLLIDHKCDIEAVD-KCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 921          
BLAST of SMED30009057 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 236.113 bits (601), Expect = 2.168e-73
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKG---------------------------------HLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG+WKNRPIHFACE+G                                 HLEIAKVLIEKKCNIEAW INNLRPIHLAC  GHAEIVTLLFDA CNIEVQ
Sbjct:  677 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQ 840          

HSP 2 Score: 181.03 bits (458), Expect = 9.854e-54
Identity = 91/131 (69.47%), Postives = 104/131 (79.39%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNL--RPIHLACWRGHAEIVTLLFDANCNIE 389
            LSHPIHLACKNGHLEIVKLLINKKCNIEACR+QNS+RAIHFACE+GHLEIVKLLIDQHCDIEAI  +   +PI  AC  GHLEI K+L+  KC+IEA    ++  R IH AC +GH  +V  L D NCN+E
Sbjct:   36 LSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVE 166          

HSP 3 Score: 147.132 bits (370), Expect = 9.689e-42
Identity = 76/126 (60.32%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+ GH +IVKLLI++KC+IE+    N +R IH ACEQGHL+IVKLLIDQ CDIE+    +K RPIH ACEKG LEI K+LI++ C+IEA   +N  PIH+AC  GH +IV LL D +C+IE
Sbjct:  274 PIHFACEQGHFDIVKLLIDQKCDIESADNSN-QRPIHVACEQGHLDIVKLLIDQKCDIES-ADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIE 397          

HSP 4 Score: 146.362 bits (368), Expect = 1.700e-41
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ G LEIVKLLI++ C+IEAC+ ++++R IH ACE GH +IVKLLIDQHCDIE+       RPI+ AC+KGHLEI K+LI + C+IE+    N RPIH+AC +GH E+V LL D +C+IE
Sbjct:  480 PIHVACEKGKLEIVKLLIDQHCDIEACK-ESNQRPIHVACEHGHFDIVKLLIDQHCDIES-ADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIE 603          

HSP 5 Score: 143.665 bits (361), Expect = 1.597e-40
Identity = 77/130 (59.23%), Postives = 98/130 (75.38%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            L  PIHLACK+G  +IVKLLI+  C+IEA R   + R IHFACE+G L+IVKLLID  CDIEA+  +  NR PIHFACE+GH +I K+LI++KC+IE+   +N RPIH+AC +GH +IV LL D  C+IE
Sbjct:  205 LVKPIHLACKHGFPDIVKLLIDHNCDIEA-RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKR--NRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIE 331          

HSP 6 Score: 142.124 bits (357), Expect = 5.580e-40
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHLEIVKLLIN+ C+IE+    N +R IH AC QGH E+VKLLIDQHCDIE+       RPI+ AC++GHL+I K+LI++KC+IE+      RPIH+AC + H EIV LL   NC+IE
Sbjct:  546 PIYVACKKGHLEIVKLLINQHCDIESADNCN-QRPIHVACNQGHFEVVKLLIDQHCDIES-ADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIE 669          

HSP 7 Score: 141.739 bits (356), Expect = 6.907e-40
Identity = 75/131 (57.25%), Postives = 94/131 (71.76%), Query Frame = 3
Query:    3 LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            L  PIH AC NGHLEI+KLLI +KC+I A   +     ++FAC +GHLEIVKLLIDQ+CD EA+  K  ++PIH AC +GHL I K+LI+ KC+IEA    N RPIH AC +GH +IV LL D NC+IE +
Sbjct:  845 LLRPIHFACNNGHLEIMKLLIAQKCDINAVN-KCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVD-KDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEAR 973          

HSP 8 Score: 139.428 bits (350), Expect = 4.193e-39
Identity = 74/127 (58.27%), Postives = 96/127 (75.59%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNR-PIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC+ GHL+IVKLLI++KC+IE+      +R IH ACE+G LEIVKLLIDQHCDIEA   K  N+ PIH ACE GH +I K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  307 PIHVACEQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKGKLEIVKLLIDQHCDIEAC--KESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIE 430          

HSP 9 Score: 138.272 bits (347), Expect = 1.074e-38
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEAC-RFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIEVQ 395
            PI LAC+NGHLEIVKLL++ KC+IEA  +   +ER+IHFAC+QGHL +VK L+DQ+C++EA   + K +PIH A  KGH++I ++LI+ KC+IEA   + ++PIHLAC  G  +IV LL D NC+IE +
Sbjct:  107 PIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEAR 234          

HSP 10 Score: 138.272 bits (347), Expect = 1.183e-38
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC++GHLEIVKLLI+QHCDIE+       RPIH AC +GH E+ K+LI++ C+IE+   +N RPI++AC +GH +IV LL D  C+IE
Sbjct:  513 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKKGHLEIVKLLINQHCDIES-ADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIE 636          

HSP 11 Score: 137.887 bits (346), Expect = 1.694e-38
Identity = 77/128 (60.16%), Postives = 94/128 (73.44%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG-KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI-NNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PI++ACK GHL+IVKLLI++KC+IE+      +R IH ACE+ HLEIVKLLI Q+CDIEA    K  + PIH AC+ GHLEI K+LI KKCNIEA    N+ R IH AC +GH EIV LL D +C+IE
Sbjct:  612 PIYVACKQGHLDIVKLLIDQKCDIESAD-NYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIE 738          

HSP 12 Score: 128.642 bits (322), Expect = 2.234e-35
Identity = 73/166 (43.98%), Postives = 97/166 (58.43%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGG----------------------------------------KWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH+AC++GH +IVKLLI++ C+IE+    N +R I+ AC+QGH +IVKLLIDQ CDIE+                                           ++K RPIH ACEKG LEI K+LI++ C+IEA   +N RPIH+AC  GH +IV LL D +C+IE
Sbjct:  373 PIHVACEHGHFDIVKLLIDQHCDIESADNSN-QRPIYVACKQGHFDIVKLLIDQKCDIESADNVQLFKTCNKSSFKYLVDVILRVQIMYICLNNVINQVINVFQYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIE 537          

HSP 13 Score: 125.946 bits (315), Expect = 1.885e-34
Identity = 69/126 (54.76%), Postives = 80/126 (63.49%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLLFDANCNIE 389
            PIH AC+  HLEI K+LI KKCNIEA    N+ R IH AC  GH EIV LL D  C+IE        RPIHFAC  GHLEI K+LI +KC+I A       P++ AC +GH EIV LL D NC+ E
Sbjct:  781 PIHFACEKDHLEIAKVLIEKKCNIEAWDI-NNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTE 905          

HSP 14 Score: 117.087 bits (292), Expect = 3.019e-31
Identity = 63/118 (53.39%), Postives = 79/118 (66.95%), Query Frame = 3
Query:   12 PIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGINNLRPIHLACWRGHAEIVTLL 365
            P++ AC  GHLEIVKLLI++ C+ EA    NS + IH AC QGHL I KLLID  CDIEA+  K   RPIHFAC +GH +I K+LI++ C+IEA      RPI +A  +   +IV LL
Sbjct:  881 PVYFACSKGHLEIVKLLIDQNCDTEAVDKDNS-KPIHQACRQGHLNITKLLIDHKCDIEAVD-KCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 996          
The following BLAST results are available for this feature:
BLAST of SMED30009057 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK12.424e-1731.75ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK12.424e-1731.75ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK12.425e-1731.75ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK39.818e-1627.78ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK39.820e-1627.78ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
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BLAST of SMED30009057 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trp-41.403e-1729.60TRP (Transient receptor potential) channel family ... [more]
mask-19.209e-1531.97Ankyrin repeat and KH domain-containing protein ma... [more]
mask-19.217e-1531.97Ankyrin repeat and KH domain-containing protein ma... [more]
mask-19.236e-1531.97Ankyrin repeat and KH domain-containing protein ma... [more]
mask-19.236e-1531.97Ankyrin repeat and KH domain-containing protein ma... [more]
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BLAST of SMED30009057 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank21.071e-1528.33gene:FBgn0261788 transcript:FBtr0303118[more]
Ank21.073e-1528.33gene:FBgn0261788 transcript:FBtr0303122[more]
Ank21.073e-1528.33gene:FBgn0261788 transcript:FBtr0344222[more]
Ank21.085e-1528.33gene:FBgn0261788 transcript:FBtr0303117[more]
Ank21.085e-1528.33gene:FBgn0261788 transcript:FBtr0303121[more]
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BLAST of SMED30009057 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a2.277e-1730.16ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ankdd1a6.722e-1732.80ankyrin repeat and death domain containing 1A [Sou... [more]
ank1b1.585e-1632.26ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.595e-1632.26ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.618e-1632.26ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of SMED30009057 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK11.222e-1732.77ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK11.222e-1732.77ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK11.223e-1732.77ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK11.225e-1732.77ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK11.226e-1732.77ankyrin 1 [Source:NCBI gene;Acc:613080][more]
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BLAST of SMED30009057 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ripk46.760e-1834.43receptor-interacting serine-threonine kinase 4 [So... [more]
Ank11.367e-1731.75ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank11.370e-1731.75ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank11.370e-1731.75ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank11.370e-1731.75ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
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BLAST of SMED30009057 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5UP11|YR848_MIMIV1.916e-1934.62Putative ankyrin repeat protein R848 OS=Acanthamoe... [more]
sp|Q5UPE2|YL063_MIMIV6.542e-1837.01Putative ankyrin repeat protein L63 OS=Acanthamoeb... [more]
sp|Q9ERK0|RIPK4_MOUSE4.729e-1734.43Receptor-interacting serine/threonine-protein kina... [more]
sp|Q02357|ANK1_MOUSE9.586e-1731.75Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV... [more]
sp|Q5UPA3|YL022_MIMIV1.086e-1630.83Putative ankyrin repeat protein L22 OS=Acanthamoeb... [more]
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BLAST of SMED30009057 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1X7SJT31.771e-2847.76ANK_REP_REGION domain-containing protein OS=Amphim... [more]
A0A182H2I61.412e-2746.03Uncharacterized protein OS=Aedes albopictus OX=716... [more]
A0A182GGY82.149e-2746.03ANK_REP_REGION domain-containing protein OS=Aedes ... [more]
A0A1X7TAL42.288e-2748.48ANK_REP_REGION domain-containing protein OS=Amphim... [more]
A0A1X7T0E55.796e-2747.37ANK_REP_REGION domain-containing protein OS=Amphim... [more]
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BLAST of SMED30009057 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a5.506e-1730.16ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b7.149e-1730.16ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ripk42.356e-1628.03receptor interacting serine/threonine kinase 4 [So... [more]
ripk43.957e-1628.03receptor interacting serine/threonine kinase 4 [So... [more]
ank3b1.755e-1526.98ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
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BLAST of SMED30009057 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002048.11.597e-1733.61pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 g... [more]
ankdd1a2.885e-1736.92ankyrin repeat and death domain containing 1A [Sou... [more]
ENSPMAT00000007954.11.362e-1532.28pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gen... [more]
ENSPMAT00000000093.14.940e-1532.52pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000000095.19.578e-1532.52pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
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BLAST of SMED30009057 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
NAS69.249e-727.42Evolutionarily conserved 19S regulatory particle a... [more]
PHO811.065e-623.94Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
HOS42.934e-631.87Subunit of the Set3 complex; complex is a meiotic-... [more]
AKR17.312e-630.68Palmitoyl transferase involved in protein palmitoy... [more]
AVO27.496e-628.91Component of a complex containing the Tor2p kinase... [more]
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BLAST of SMED30009057 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO281782.298e-1635.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460991.780e-1128.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387024.153e-1129.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348235.626e-1134.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387251.782e-930.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30009057 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a2.219e-1833.87ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ank1b5.129e-1733.06ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ripk48.882e-1729.14receptor interacting serine/threonine kinase 4 [So... [more]
ENSORLT00000040233.11.680e-1528.57pep primary_assembly:ASM223467v1:19:16412534:16434... [more]
ankhd11.912e-1531.97ankyrin repeat and KH domain-containing protein 1 ... [more]
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BLAST of SMED30009057 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017135.11.283e-7375.61SMESG000017135.1[more]
SMESG000017135.11.380e-7375.61SMESG000017135.1[more]
SMESG000017135.11.636e-7375.61SMESG000017135.1[more]
SMESG000017135.11.794e-7375.61SMESG000017135.1[more]
SMESG000017135.12.168e-7375.61SMESG000017135.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009057 ID=SMED30009057|Name=SMED30009057|organism=Schmidtea mediterranea sexual|type=transcript|length=396bp
GTTTATCACATCCAATACATTTAGCCTGTAAAAACGGGCACCTGGAAATT
GTTAAACTCCTCATAAATAAAAAGTGTAACATTGAGGCATGCAGATTTCA
AAATTCTGAACGCGCAATTCATTTCGCATGCGAGCAAGGCCATTTAGAGA
TTGTGAAACTGCTGATCGATCAACATTGTGATATTGAGGCAATTGGTGGC
AAATGGAAAAATCGCCCAATTCATTTTGCCTGTGAGAAAGGTCACTTGGA
AATTGCAAAAGTTCTGATTGAGAAAAAGTGTAATATTGAGGCGTGGGGTA
TTAATAATTTACGACCGATTCATCTGGCTTGTTGGCGGGGCCACGCAGAA
ATAGTTACGCTATTGTTCGATGCAAATTGCAACATTGAGGTTCAGG
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protein sequence of SMED30009057-orf-1

>SMED30009057-orf-1 ID=SMED30009057-orf-1|Name=SMED30009057-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=131bp
LSHPIHLACKNGHLEIVKLLINKKCNIEACRFQNSERAIHFACEQGHLEI
VKLLIDQHCDIEAIGGKWKNRPIHFACEKGHLEIAKVLIEKKCNIEAWGI
NNLRPIHLACWRGHAEIVTLLFDANCNIEVQ
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 34..63
e-value: 0.015
score: 24.4
coord: 1..29
e-value: 0.024
score: 23.8
coord: 101..130
e-value: 0.047
score: 22.8
coord: 68..97
e-value: 0.19
score: 20.9
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 34..66
score: 9.912
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 101..131
score: 9.698
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 1..32
score: 9.431
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 34..131
e-value: 4.4E-27
score: 96.8
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 3..130
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 4..62
e-value: 3.1E-13
score: 50.1
coord: 69..129
e-value: 6.4E-10
score: 39.5
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 1..131
score: 31.511
NoneNo IPR availableGENE3DG3DSA:1.25.40.960coord: 2..33
e-value: 5.6E-7
score: 31.6
NoneNo IPR availablePANTHERPTHR24188ANKYRIN REPEAT PROTEINcoord: 4..63
coord: 37..130