SMED30009051
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009051 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30009051 aligns in the following genomic locations:
Homology
BLAST of SMED30009051 vs. Ensembl Zebrafish
Match: FO704673.1 (pep chromosome:GRCz11:12:14545475:14551077:-1 gene:ENSDARG00000112601.1 transcript:ENSDART00000188717.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:FO704673.1) HSP 1 Score: 43.8986 bits (102), Expect = 5.942e-11 Identity = 24/71 (33.80%), Postives = 39/71 (54.93%), Query Frame = -1 Query: 659 VTLKSISAEIVANAILQQ*IFR-YGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERL 868 + LK + + L Q +FR YG+P+ I DR QF S++++ ++L I ++ YH + NG ERL Sbjct: 1085 IPLKGLPTAMETALELFQHVFRVYGIPEDIVSDRGPQFTSKVWKAFCKQLDINVSLTSGYHPESNGQVERL 1155 HSP 2 Score: 43.1282 bits (100), Expect = 5.942e-11 Identity = 26/95 (27.37%), Postives = 39/95 (41.05%), Query Frame = -2 Query: 889 HATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKP------PIIHYPTGNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1155 H + H G S L+ + WP M ++ +V C C Q K + PI H P + L D V LP N ++ +L +ID+ Sbjct: 987 HNSLSSGHPGISRTLNLVRNAFWWPKMNQDITTFVKSCAVCAQSKTPRELPSGLLQPLPIPHRP----WSHLSIDFVTDLPNSNNYTTILVIIDR 1077
BLAST of SMED30009051 vs. Ensembl Zebrafish
Match: BX571665.1 (pep chromosome:GRCz11:17:35431724:35437222:1 gene:ENSDARG00000111789.1 transcript:ENSDART00000190293.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX571665.1) HSP 1 Score: 41.5874 bits (96), Expect = 4.598e-9 Identity = 21/64 (32.81%), Postives = 36/64 (56.25%), Query Frame = -1 Query: 659 SAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERL 850 +AE + N + + +G+P I DR +QF S+++ L + L I ++ YH + NG TER+ Sbjct: 1101 TAEALCNHVFRM----FGLPDDIVSDRGSQFTSRVWSALCKNLNINVSLTSGYHPQSNGQTERM 1160 HSP 2 Score: 38.891 bits (89), Expect = 4.598e-9 Identity = 23/92 (25.00%), Postives = 43/92 (46.74%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIH-YPTGNR-FETLHFDLVGPLPPDNGFSYLLTMIDK 1158 H T H G +A L+ ++ WP++ +V +V +C CQ K + T ++ P R + + D + LP + +LT++D+ Sbjct: 991 IHDTPSSGHPGITATLELVKARFWWPSLTKDVINYVQKCKICQSTKASHQTPAGLLQPLPVPQRPWSHIAVDFITDLPVSQNNTVILTVVDR 1082
BLAST of SMED30009051 vs. Ensembl Zebrafish
Match: CU929458.1 (pep chromosome:GRCz11:11:43363075:43368382:1 gene:ENSDARG00000114395.1 transcript:ENSDART00000179934.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU929458.1) HSP 1 Score: 41.5874 bits (96), Expect = 4.905e-9 Identity = 21/64 (32.81%), Postives = 36/64 (56.25%), Query Frame = -1 Query: 659 SAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERL 850 +AE + N + + +G+P I DR +QF S+++ L + L I ++ YH + NG TER+ Sbjct: 1108 TAEALCNHVFRM----FGLPDDIVSDRGSQFTSRVWSALCKNLNINVSLTSGYHPQSNGQTERM 1167 HSP 2 Score: 38.5058 bits (88), Expect = 4.905e-9 Identity = 23/92 (25.00%), Postives = 43/92 (46.74%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIH-YPTGNR-FETLHFDLVGPLPPDNGFSYLLTMIDK 1158 H T H G +A L+ ++ WP++ +V +V +C CQ K + T ++ P R + + D + LP + +LT++D+ Sbjct: 998 IHDTPSSGHPGITATLELVKARFWWPSLTKDVINYVQKCEICQSTKASHQTPAGLLQPLPVPQRPWSHIAVDFITDLPVSQNNTVILTVVDR 1089
BLAST of SMED30009051 vs. Ensembl Zebrafish
Match: CR354395.2 (pep chromosome:GRCz11:12:13553730:13556536:-1 gene:ENSDARG00000101547.2 transcript:ENSDART00000166396.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR354395.2) HSP 1 Score: 39.6614 bits (91), Expect = 1.851e-8 Identity = 20/64 (31.25%), Postives = 35/64 (54.69%), Query Frame = -1 Query: 659 SAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERL 850 +AE + N + + +G+P I DR +QF S+++ L + L I ++ YH + NG TE + Sbjct: 766 TAEALCNHVFRM----FGLPDDIVSDRGSQFTSRVWSALCKNLNINVSLTSGYHPQSNGQTEHM 825 HSP 2 Score: 38.891 bits (89), Expect = 1.851e-8 Identity = 23/92 (25.00%), Postives = 43/92 (46.74%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIH-YPTGNR-FETLHFDLVGPLPPDNGFSYLLTMIDK 1158 H T H G +A L+ ++ WP++ +V +V +C CQ K + T ++ P R + + D + LP + +LT++D+ Sbjct: 656 IHDTPSSGHPGITATLELVKARFWWPSLTKDVINYVQKCKICQSTKASHQTPAGLLQPLPVPQRPWSHIAVDFITDLPVSQNNTVILTVVDR 747
BLAST of SMED30009051 vs. Ensembl Zebrafish
Match: CR533578.3 (pep chromosome:GRCz11:16:23466094:23471387:-1 gene:ENSDARG00000115891.1 transcript:ENSDART00000180899.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR533578.3) HSP 1 Score: 38.891 bits (89), Expect = 3.079e-8 Identity = 20/52 (38.46%), Postives = 32/52 (61.54%), Query Frame = -1 Query: 659 IFR-YGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERL 811 +FR +G+P I DR +QF S+++ L + L I ++ YH + NG TER+ Sbjct: 1116 VFRMFGLPDDIVSDRGSQFTSRVWSALCKNLNINVSLTSGYHPQSNGQTERM 1167 HSP 2 Score: 38.5058 bits (88), Expect = 3.079e-8 Identity = 23/92 (25.00%), Postives = 43/92 (46.74%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIH-YPTGNR-FETLHFDLVGPLPPDNGFSYLLTMIDK 1158 H T H G +A L+ ++ WP++ +V +V +C CQ K + T ++ P R + + D + LP + +LT++D+ Sbjct: 998 IHDTPSSGHPGITATLELVKARFWWPSLTKDVINYVQKCKICQSTKASHQTPAGLLQPLPVPQRPWSHIAVDFITDLPVSQNNTVILTVVDR 1089
BLAST of SMED30009051 vs. Ensembl Xenopus
Match: ENSXETT00000023941.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV463742.1:21:4282:-1 gene:ENSXETG00000015734.1 transcript:ENSXETT00000023941.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 1.150e-16 Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = -1 Query: 587 RLFEAVTLKSISAEIVANAILQQ*IF-RYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKL--YLLF 883 R EAV L+ I A VA+A++Q IF R G P I D+ QF SQL Q L ++ ++ S+ YH + NG+ ER TLKT + S + +W + +LLF Sbjct: 739 RYPEAVALRKIDAPTVADALIQ--IFSRVGFPSEILSDQGPQFTSQLLQCLWQRCGVRAIHSSPYHPQTNGLCERFNGTLKTMLRTFVESGEKDWERYLPHLLF 840 HSP 2 Score: 41.2022 bits (95), Expect = 1.150e-16 Identity = 31/106 (29.25%), Postives = 45/106 (42.45%), Query Frame = -2 Query: 877 FHATHDL---HHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKP-------PIIHYPTGNRFETLHFDLVGPL--PPDNGFSYLLTMIDKKGVY 1158 H H++ H G + L WP + EV + C CQ+ + KP PII P F+ + DL+GPL P +G Y+LT++D Y Sbjct: 640 LHMAHEIPLAGHQGVTRTRHRLTQNFYWPGISQEVTRYCRTCDSCQRTG-RANDKPKFPLCPLPIISEP----FQRVAVDLIGPLSRPSRSGKQYILTVMDYATRY 740
BLAST of SMED30009051 vs. Ensembl Xenopus
Match: ENSXETT00000025355.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:22781891:22783779:1 gene:ENSXETG00000014447.1 transcript:ENSXETT00000025355.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 7.790e-15 Identity = 31/96 (32.29%), Postives = 47/96 (48.96%), Query Frame = -1 Query: 602 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILS--SKVNWTK 883 R +A K A VA ++++ YG+PK I D+ FES+L +L L + R+T YH + + ER RTL G L K +W++ Sbjct: 217 RYAQAFPTKDQRAITVAKVLVERFFIHYGLPKRIHSDQGRDFESKLVHELMSMLGVLKSRTTPYHPQGDPQPERFNRTLLDMLGTLPKEKKTHWSR 312 HSP 2 Score: 48.521 bits (114), Expect = 7.790e-15 Identity = 24/88 (27.27%), Postives = 41/88 (46.59%), Query Frame = -2 Query: 892 ATHDLH-HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNRFETLHFDLVGPLPPDNGFSYLLTMID 1152 A HD H HLG+ L+ + WP M+ +V+ + C+ C Q K P+ H + + + + D + P + G S +L + D Sbjct: 126 ALHDEHGHLGYDKTLGLVRDRFYWPCMKQDVEDYCRSCLRCIQRKTLPSRAAPLSHMESHSPLDLVCIDFLSIEPDEGGTSNVLVVTD 213
BLAST of SMED30009051 vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 1.441e-14 Identity = 31/96 (32.29%), Postives = 47/96 (48.96%), Query Frame = -1 Query: 602 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILS--SKVNWTK 883 R +A K A VA ++++ YG+PK I D+ FES+L +L L + R+T YH + + ER RTL G L K +W++ Sbjct: 1004 RYAQAFPTKDQRAITVAKVLVERFFIHYGLPKRIHSDQGRDFESKLVHELMSMLGVLKSRTTPYHPQGDPQPERFNRTLLDMLGTLPKEKKTHWSR 1099 HSP 2 Score: 47.7506 bits (112), Expect = 1.441e-14 Identity = 24/88 (27.27%), Postives = 41/88 (46.59%), Query Frame = -2 Query: 892 ATHDLH-HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNRFETLHFDLVGPLPPDNGFSYLLTMID 1152 A HD H HLG+ L+ + WP M+ +V+ + C+ C Q K P+ H + + + + D + P + G S +L + D Sbjct: 913 ALHDEHGHLGYDKTLGLVRDRFYWPCMKQDVEDYCRSCLRCIQRKTLPSRAAPLSHMESHSPLDLVCIDFLSIEPDEGGTSNVLVVTD 1000
BLAST of SMED30009051 vs. Ensembl Xenopus
Match: mybl2 (MYB proto-oncogene like 2 [Source:Xenbase;Acc:XB-GENE-982419]) HSP 1 Score: 55.4546 bits (132), Expect = 5.034e-14 Identity = 33/107 (30.84%), Postives = 60/107 (56.07%), Query Frame = -1 Query: 572 RLFEAVTLKSISAEIVANAILQQ*IF-RYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKLYLLFFLVGA 883 R EA+ L++ S++ +A ++ +F R G+PK I D+ T F S++ ++L + R++ YH + +G+ ER +TLK+ + + NW YLL +L+ A Sbjct: 176 RYPEAIPLRNTSSKSIARELVH--VFSRVGIPKEILTDQGTPFMSRVMKELCRLFKVVQLRTSVYHPQTDGLVERFNKTLKSMLKKVVDKDGKNWD--YLLPYLMFA 278 HSP 2 Score: 42.743 bits (99), Expect = 5.034e-14 Identity = 25/88 (28.41%), Postives = 39/88 (44.32%), Query Frame = -2 Query: 877 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPL-PPDNGFSYLLTMIDKKGVY 1134 HLG + + WP + EV+ + S C CQ H + P++ P FE + DLVGP+ G ++L ++D Y Sbjct: 90 HLGVEKTKERILQRFFWPGLHGEVEQYCSSCPDCQYSAPRPHFRSPLVPLPVIETPFERIAMDLVGPIVKSARGHQHILVIMDYATRY 177
BLAST of SMED30009051 vs. Ensembl Xenopus
Match: nmbr (neuromedin B receptor [Source:Xenbase;Acc:XB-GENE-6074611]) HSP 1 Score: 52.373 bits (124), Expect = 4.961e-13 Identity = 26/82 (31.71%), Postives = 49/82 (59.76%), Query Frame = -1 Query: 641 RLFEAVTLKSISAEIVANAILQQ*IF-RYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT 883 R EA+ L++ S++ +A ++ +F R G+PK I D+ T F S++ ++L + R++ YH + +G+ ER +TLK+ Sbjct: 243 RYPEAIPLRNTSSKSIARELVH--VFSRVGIPKEILTDQGTPFMSRVMKELCRLFKVVQLRTSVYHPQTDGLVERFNKTLKS 322 HSP 2 Score: 42.3578 bits (98), Expect = 4.961e-13 Identity = 24/83 (28.92%), Postives = 38/83 (45.78%), Query Frame = -2 Query: 892 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPL-PPDNGFSYLLTMID 1134 HLG + + WP + EV+ + S C CQ H + P++ P FE + DLVGP+ G ++L ++D Sbjct: 157 HLGVEKTKERILQRFFWPGLHGEVEQYCSSCPDCQYSAPRPHFRSPLVPLPVIETPFERIAMDLVGPIVKSARGHQHILVIMD 239
BLAST of SMED30009051 vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 4.688e-11 Identity = 31/99 (31.31%), Postives = 56/99 (56.57%), Query Frame = -1 Query: 581 AVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKLYLLFFL 871 A+ + + SA+ VA AI + I +YG K+ D T++++ + L + L I++ STA+H + G+ ER +RTL I + K +W ++L +F+ Sbjct: 999 AIPIANKSAKTVAKAIFESFILKYGPMKTFITDMGTEYKNSIITDLCKYLKIKNITSTAHHHQTVGVVERSHRTLNEYIRSYISTDKTDW-DVWLQYFV 1096 HSP 2 Score: 37.7354 bits (86), Expect = 4.688e-11 Identity = 30/89 (33.71%), Postives = 43/89 (48.31%), Query Frame = -2 Query: 874 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNR-FETLHFDLVGPLP-PDNGFSYLLTMIDKKGVYL 1134 H G + + W M +K +V +C CQ+ K TKHTK P+ T F+ + D +GPLP +NG Y +T+I YL Sbjct: 909 HTGITKTLAKVKRHYYWKNMSKYIKEYVRKCQKCQKAKTTKHTKTPMTITETPEHAFDRVVVDTIGPLPKSENGNEYAVTLICDLTKYL 997
BLAST of SMED30009051 vs. UniProt/SwissProt
Match: sp|P23074|POL_SFV1 (Pro-Pol polyprotein OS=Simian foamy virus type 1 OX=338478 GN=pol PE=1 SV=3) HSP 1 Score: 60.4622 bits (145), Expect = 7.349e-11 Identity = 30/91 (32.97%), Postives = 46/91 (50.55%), Query Frame = -2 Query: 892 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTG--NRFETLHFDLVGPLPPDNGFSYLLTMID 1158 H++ H G A F + S+ WP +R +V + QC C T T PPI+ P F+ + D +GPLPP NG+ ++L ++D Sbjct: 808 ISTAHNIAHTGRDATFLKVSSKYWWPNLRKDVVKSIRQCKQCLVTNATNLTSPPILR-PVKPLKPFDKFYIDYIGPLPPSNGYLHVLVVVD 897 HSP 2 Score: 28.8758 bits (63), Expect = 7.349e-11 Identity = 15/46 (32.61%), Postives = 22/46 (47.83%), Query Frame = -1 Query: 662 GVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITER 799 +PK + D+ F S F ++ IQ ST YH + +G ER Sbjct: 927 AIPKVLHSDQGAAFTSSTFADWAKEKGIQLEFSTPYHPQSSGKVER 972
BLAST of SMED30009051 vs. UniProt/SwissProt
Match: sp|P27401|POL_SFV3L (Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE=3 SV=2) HSP 1 Score: 54.6842 bits (130), Expect = 1.328e-9 Identity = 26/87 (29.89%), Postives = 43/87 (49.43%), Query Frame = -2 Query: 892 THDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNR-FETLHFDLVGPLPPDNGFSYLLTMID 1149 H++ H G + F + S+ WP +R +V + QC C PPI+ + F+ D +GPLPP NG+ ++L ++D Sbjct: 811 AHNIAHTGRDSTFLKVSSKYWWPNLRKDVVKVIRQCKQCLVTNAATLAAPPILRPERPVKPFDKFFIDYIGPLPPSNGYLHVLVVVD 897 HSP 2 Score: 30.4166 bits (67), Expect = 1.328e-9 Identity = 17/46 (36.96%), Postives = 21/46 (45.65%), Query Frame = -1 Query: 662 GVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITER 799 VPK I D+ F S F + IQ ST YH + +G ER Sbjct: 927 AVPKVIHSDQGAAFTSATFADWAKNKGIQLEFSTPYHPQSSGKVER 972
BLAST of SMED30009051 vs. UniProt/SwissProt
Match: sp|Q87040|POL_SFVCP (Pro-Pol polyprotein OS=Simian foamy virus (isolate chimpanzee) OX=298339 GN=pol PE=3 SV=1) HSP 1 Score: 52.373 bits (124), Expect = 2.199e-8 Identity = 26/86 (30.23%), Postives = 41/86 (47.67%), Query Frame = -2 Query: 892 HDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNR-FETLHFDLVGPLPPDNGFSYLLTMID 1146 H+L H G A + + WP MR +V + +C C + T PI+ + F+ D +GPLPP G+ Y+L ++D Sbjct: 813 HNLAHTGREATLLKIANLYWWPNMRKDVVKQLGRCKQCLITNASNKTSGPILRPDRPQKPFDKFFIDYIGPLPPSQGYLYVLVIVD 898 HSP 2 Score: 28.4906 bits (62), Expect = 2.199e-8 Identity = 15/46 (32.61%), Postives = 22/46 (47.83%), Query Frame = -1 Query: 662 GVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITER 799 +PK I D+ F S F + ++ I ST YH + +G ER Sbjct: 928 AIPKVIHSDQGAAFTSSTFAEWAKERGIHLEFSTPYHPQSSGKVER 973
BLAST of SMED30009051 vs. UniProt/SwissProt
Match: sp|O93209|POL_FFV (Pro-Pol polyprotein OS=Feline foamy virus OX=53182 GN=pol PE=3 SV=1) HSP 1 Score: 50.0618 bits (118), Expect = 2.607e-8 Identity = 25/91 (27.47%), Postives = 43/91 (47.25%), Query Frame = -2 Query: 892 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHK--ITKHTKPPIIHYPTGNRFETLHFDLVGPLPPDNGFSYLLTMID 1158 H++ H G A + WP M+ ++ ++S C C+ K P I +PT F+ + D +GPLPP G+ ++L ++D Sbjct: 810 IKEAHNISHAGREAVLLKIQENYWWPKMKKDISSFLSTCNVCKMVNPLNLKPISPQAIVHPT-KPFDKFYMDYIGPLPPSEGYVHVLVVVD 899 HSP 2 Score: 30.4166 bits (67), Expect = 2.607e-8 Identity = 16/46 (34.78%), Postives = 25/46 (54.35%), Query Frame = -1 Query: 662 GVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITER 799 +PK + D+ + F S+ F Q ++ IQ ST YH + +G ER Sbjct: 929 AIPKVLHSDQGSAFTSEEFAQWAKERNIQLEFSTPYHPQSSGKVER 974
BLAST of SMED30009051 vs. TrEMBL
Match: A0A139W8U7 (Uncharacterized protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC031725 PE=4 SV=1) HSP 1 Score: 93.2041 bits (230), Expect = 1.394e-35 Identity = 41/91 (45.05%), Postives = 57/91 (62.64%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1158 F+ H+L H G +A ++ + +WP + + W QCI CQ+ KIT+HTK +PT NRF LH DLVGPLPP +G+ Y+LT ID+ Sbjct: 933 FYNVHNLSHPGVNATTKMVKQKYMWPGIDKNCRTWARQCISCQRSKITRHTKSNFGSFPTPTNRFRHLHLDLVGPLPPSHGYKYVLTCIDR 1023 HSP 2 Score: 87.0409 bits (214), Expect = 1.394e-35 Identity = 44/101 (43.56%), Postives = 65/101 (64.36%), Query Frame = -1 Query: 581 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNWTKLYLLFFL 883 R EA+ +K ISAE VA A+L I R+G+P+ I DR TQF+S+LF+ +++ I H ++TAYH + NG+ ERL++ LKTS +++ NW L L Sbjct: 1026 RWPEAIPIKDISAETVAQALLHTWIARFGIPELITTDRGTQFDSELFRNMSKLFGICHCKTTAYHPQANGMIERLHQQLKTSIKCHNTE-NWFDCLPLVLL 1125
BLAST of SMED30009051 vs. TrEMBL
Match: A0A430QE18 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0010991 PE=4 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 3.035e-35 Identity = 38/91 (41.76%), Postives = 56/91 (61.54%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYP-TGNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1158 F A H++ H G A L+ + +WP+M +++ W CI CQ+ KI +HT + H+ NRFE +H D++GPLP NGFSY+ T ID+ Sbjct: 656 FEAMHNISHPGIKATIKLISERFVWPSMNRDIRNWTQACIKCQRVKIHRHTSSAVGHFRHPDNRFEHVHIDIIGPLPVSNGFSYIFTCIDR 746 HSP 2 Score: 88.5817 bits (218), Expect = 3.035e-35 Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = -1 Query: 584 AVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNW-TKLYLLFF 871 AV +K I+AE +A +++ I YGVP +I DR QFES+LFQQLTE L I+ R+T+YH NG+ ERL+R LK S + S +W +KL L+ Sbjct: 753 AVPIKDITAETIAKTLVEHWISHYGVPTNITTDRGAQFESRLFQQLTELLGIKRIRTTSYHPMSNGMVERLHRQLKASLTAVISNNDWASKLPLVML 849
BLAST of SMED30009051 vs. TrEMBL
Match: A0A430QDQ5 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0012774 PE=4 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 3.163e-35 Identity = 38/91 (41.76%), Postives = 56/91 (61.54%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYP-TGNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1158 F A H++ H G A L+ + +WP+M +++ W CI CQ+ KI +HT + H+ NRFE +H D++GPLP NGFSY+ T ID+ Sbjct: 656 FEAMHNISHPGIKATIKLISERFVWPSMNRDIRNWTQACIKCQRVKIHRHTSSAVGHFRHPDNRFEHVHIDIIGPLPVSNGFSYIFTCIDR 746 HSP 2 Score: 88.5817 bits (218), Expect = 3.163e-35 Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = -1 Query: 584 AVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNW-TKLYLLFF 871 AV +K I+AE +A +++ I YGVP +I DR QFES+LFQQLTE L I+ R+T+YH NG+ ERL+R LK S + S +W +KL L+ Sbjct: 753 AVPIKDITAETIAKTLVEHWISHYGVPTNITTDRGAQFESRLFQQLTELLGIKRIRTTSYHPMSNGMVERLHRQLKASLTAVISNNDWASKLPLVML 849
BLAST of SMED30009051 vs. TrEMBL
Match: A0A085MC24 (Uncharacterized protein (Fragment) OS=Trichuris suis OX=68888 GN=M513_04470 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 1.111e-34 Identity = 38/96 (39.58%), Postives = 57/96 (59.38%), Query Frame = -2 Query: 889 FT*ISFHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1173 F I F+ H L H G A LL + +WP+++ + W +C+ CQ+ KI +H + P+ ++P RF +H D+VGPLPP G SY+LT ID+ Sbjct: 555 FRKIVFNHLHSLSHPGTRATSRLLRERFVWPSIKKDCATWTRECLTCQKAKIHRHVRSPLANFPVPAERFSHVHIDIVGPLPPAQGHSYILTCIDR 650 HSP 2 Score: 83.5741 bits (205), Expect = 1.111e-34 Identity = 45/101 (44.55%), Postives = 63/101 (62.38%), Query Frame = -1 Query: 581 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNWTKLYLLFFL 883 R EA+ ++ SAE +A A LQ + R+GVP++I D+ QFES LF LT+ L I+ R+TAY+ K NG+ ER++R LKT+ I K NW + L L Sbjct: 653 RWPEAMPIEDASAETIAFAFLQGWVSRFGVPRTITTDQGRQFESSLFNSLTQFLGIRRTRTTAYNPKANGLVERMHRQLKTAI-ICHKKANWLQALPLVLL 752 HSP 3 Score: 25.0238 bits (53), Expect = 1.111e-34 Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = -3 Query: 540 QALPIVLLGWRSTPVRTTGTSI 605 QALP+VLLG RS G S+ Sbjct: 745 QALPLVLLGMRSVVKEDLGFSV 766 HSP 4 Score: 23.483 bits (49), Expect = 1.111e-34 Identity = 26/97 (26.80%), Postives = 47/97 (48.45%), Query Frame = -2 Query: 241 DNSPTLDELDTARDHVFSIDSNPQFTTASSYTPYVPKSFKDAKNVNVSNVTDSGFKKR----YYGPYKLXXXXXXXXXXXXNGVAQTINL*QLKPAL 519 D S + ++ TA + + +N + TP+V K + +V V G KK YYGP+++I+ N ++++G + + L +LKPA Sbjct: 791 DRSRLISDIRTAIQRMVPVPTNHH----GNPTPFVHKDLMSSSHVLVRK---GGVKKPLTAPYYGPFRVIERSKNFFTLDMHGEHRVVCLDRLKPAF 880
BLAST of SMED30009051 vs. TrEMBL
Match: A0A5S6QCX0 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 83.9593 bits (206), Expect = 2.708e-34 Identity = 38/92 (41.30%), Postives = 56/92 (60.87%), Query Frame = -2 Query: 889 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHY--PTGNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1158 F H+L H G A L+ + +WP+++ + W +C+ CQ KI KHT+ P+ + PT RF +H D+VGPLPP G+SY+LT ID+ Sbjct: 906 FSHLHNLSHPGIRATSQLIRQRFVWPSIKKDCTTWTRECLACQTAKIHKHTRSPLGKFKVPT-ERFSHVHIDIVGPLPPAQGYSYILTCIDR 996 HSP 2 Score: 82.8037 bits (203), Expect = 2.708e-34 Identity = 43/101 (42.57%), Postives = 62/101 (61.39%), Query Frame = -1 Query: 581 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNWTKLYLLFFL 883 R EA+ +++ S E +A A L + R+GVPK++ D+ QFES +F LTE L I+H R+TAY+ K NG+ ER++R LKT+ K NW + L L Sbjct: 999 RWPEAIPIQNTSTETIALAFLHAWVSRFGVPKTLTTDQGRQFESSMFNHLTEFLGIKHTRTTAYNPKANGLVERMHRQLKTAI-TCHKKDNWLQALPLVLL 1098 HSP 3 Score: 25.0238 bits (53), Expect = 2.708e-34 Identity = 29/103 (28.16%), Postives = 42/103 (40.78%), Query Frame = -2 Query: 241 LIDFDNSPTLDELDTARDHVFSIDSNPQFTTA--SSYTPYVPKSFKDAKNVNVSNVTDSGFKKR----YYGPYKLXXXXXXXXXXXXNGVAQTINL*QLKPAL 531 I PT D D +I TT+ S TP+V K +V + G KK Y GPY++I+ +++ G + I L +LKPA Sbjct: 1127 FIATSKQPTGTGFDLVSDIRIAIQKMIPTTTSHHGSLTPFVHKDLMSTSHVFIRK---GGVKKPLTTPYDGPYRVIERNTKFFTLDMRGKQRVIGLDRLKPAF 1226 HSP 4 Score: 24.2534 bits (51), Expect = 2.708e-34 Identity = 11/24 (45.83%), Postives = 14/24 (58.33%), Query Frame = -3 Query: 534 QALPIVLLGWRSTPVRTTGTSICN 605 QALP+VLLG R+ G S+ Sbjct: 1091 QALPLVLLGMRAAVKEDIGFSVAE 1114
BLAST of SMED30009051 vs. Ensembl Cavefish
Match: ENSAMXT00000037741.1 (pep primary_assembly:Astyanax_mexicanus-2.0:6:27788674:27792885:-1 gene:ENSAMXG00000029895.1 transcript:ENSAMXT00000037741.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.4774 bits (171), Expect = 7.879e-19 Identity = 35/94 (37.23%), Postives = 57/94 (60.64%), Query Frame = -1 Query: 599 EAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKL 874 EA +++ A+ VA +++Q I R+G P+SI D+ F+S LF+ + E L I R+T YH +G+ ERL RT+ T S + +++ NW +L Sbjct: 190 EAFAMRAQDADTVARTVVEQFILRWGTPRSIHTDQGKNFDSALFRAICELLDIHKTRTTPYHPSSDGLVERLNRTITTMLSMFVDANQGNWDEL 283 HSP 2 Score: 42.743 bits (99), Expect = 7.879e-19 Identity = 25/91 (27.47%), Postives = 39/91 (42.86%), Query Frame = -2 Query: 892 FHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKIT-KHTKPPIIHYPTGNRFETLHFDLVGPLP-PDNGFSYLLTMID 1158 H + HLG + + ++ WP +V W +C C K + + P+ G FE L D+VGPLP G Y++ + D Sbjct: 93 LHNSKTGGHLGIAKLKDKIRTRFYWPKWSSDVMDWCKKCELCAVFKTSGAEPRAPLRPIQAGYPFEKLGIDIVGPLPVTSQGNKYIIVIAD 183
BLAST of SMED30009051 vs. Ensembl Cavefish
Match: ENSAMXT00000033239.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002121.1:47256:48953:1 gene:ENSAMXG00000036506.1 transcript:ENSAMXT00000033239.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 54.299 bits (129), Expect = 1.315e-16 Identity = 28/93 (30.11%), Postives = 49/93 (52.69%), Query Frame = -1 Query: 602 EAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKV--NWTK 874 EA + A A+ ++++ R+GVP+ + D+ FES++ ++ L I R+T H + +G+ ER RTL ++SSK +W K Sbjct: 264 EAYAVPDQGAVTTADVLVREFFCRFGVPEELHSDQGRNFESEVMAEVCRILGIHKTRTTPLHPQSDGLVERFNRTLAAQLAMVSSKGQRDWDK 356 HSP 2 Score: 47.3654 bits (111), Expect = 1.315e-16 Identity = 26/90 (28.89%), Postives = 41/90 (45.56%), Query Frame = -2 Query: 892 HATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKI-TKHTKPPIIHYPTGNRFETLHFDLVGPLP-PDNGFSYLLTMID 1155 H + H G + L + WP R +V+L+V C C K + + P+ Y G E + D++GP P D+G Y+L +D Sbjct: 168 HGSPGAGHFGVTKTLKRLRQRFYWPGCRTDVELFVHCCDVCAAKKGPARAPRAPLHPYQCGGPMERVAVDVLGPFPVTDSGNRYVLVAMD 257 HSP 3 Score: 23.8682 bits (50), Expect = 1.315e-16 Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = -3 Query: 549 DQALPIVLLGWRSTPVRTTG 608 D+ LP+VLL RS TTG Sbjct: 355 DKHLPVVLLACRSAVQETTG 374
BLAST of SMED30009051 vs. Ensembl Cavefish
Match: ENSAMXT00000039359.1 (pep primary_assembly:Astyanax_mexicanus-2.0:5:19359511:19362411:1 gene:ENSAMXG00000043892.1 transcript:ENSAMXT00000039359.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 62.3882 bits (150), Expect = 7.802e-16 Identity = 34/104 (32.69%), Postives = 63/104 (60.58%), Query Frame = -1 Query: 584 RLFEAVTLKSISAEIVANAILQQ*IFR-YGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTS--RGILSSKVNWTK-LYLLFF 883 R EA+ L+ I+A ++ A+++ F +G+P+ ++ D+ T F S++FQQ+ + L I+H S+ YH + G ER +++LK++ R L + W + + LL F Sbjct: 128 RFPEAIPLRKITAPVIVRALVK--FFSTFGLPREVQTDQGTNFMSKIFQQVMKMLNIKHVVSSPYHPEFQGALERFHQSLKSALKRYCLETGKGWDEGVPLLLF 229 HSP 2 Score: 40.817 bits (94), Expect = 7.802e-16 Identity = 25/84 (29.76%), Postives = 39/84 (46.43%), Query Frame = -2 Query: 895 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT---GNRFETLHFDLVGPLPPD-NGFSYLLTMI 1134 HLG + ++ + WP+++ +V + C CQ PP P G FE + D VGPLP +G +LLT++ Sbjct: 40 HLGVTKTYSRVLKCFFWPSLKKDVAEYCRTCHTCQCVGKPNQKIPPAPLCPVPVMGEPFERVLVDCVGPLPKSKSGNQFLLTIM 123
BLAST of SMED30009051 vs. Ensembl Cavefish
Match: ENSAMXT00000042253.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000503.1:15658:18693:-1 gene:ENSAMXG00000039114.1 transcript:ENSAMXT00000042253.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.2174 bits (121), Expect = 1.023e-15 Identity = 27/93 (29.03%), Postives = 48/93 (51.61%), Query Frame = -1 Query: 602 EAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKV--NWTK 874 EA + A A+ ++++ R+GVP+ + D+ FES++ ++ L I R+T H + +G+ ER TL ++SSK +W K Sbjct: 778 EAYAVPDQGAVTTADVLVREFFCRFGVPEELHSDQGRNFESEVMAEVCRILGIHKTRTTPLHPQSDGLVERFNCTLAAQLAMVSSKGQRDWDK 870 HSP 2 Score: 46.9802 bits (110), Expect = 1.023e-15 Identity = 26/90 (28.89%), Postives = 41/90 (45.56%), Query Frame = -2 Query: 892 HATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKI-TKHTKPPIIHYPTGNRFETLHFDLVGPLP-PDNGFSYLLTMID 1155 H + H G + L + WP R +V+L+V C C K + + P+ Y G E + D++GP P D+G Y+L +D Sbjct: 682 HGSPGAGHFGVTKTLKRLRQRFYWPGCRTDVELFVHCCDVCAAKKGPARAPRAPLHPYQCGGPMERVAVDVLGPFPVTDSGNRYVLVAMD 771 HSP 3 Score: 24.2534 bits (51), Expect = 1.023e-15 Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = -3 Query: 549 DQALPIVLLGWRSTPVRTTG 608 D+ LP+VLL RS TTG Sbjct: 869 DKHLPVVLLACRSAVQETTG 888
BLAST of SMED30009051 vs. Ensembl Cavefish
Match: ENSAMXT00000036301.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:28817284:28820114:1 gene:ENSAMXG00000037673.1 transcript:ENSAMXT00000036301.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 56.225 bits (134), Expect = 1.127e-15 Identity = 29/100 (29.00%), Postives = 53/100 (53.00%), Query Frame = -1 Query: 581 EAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSK--VNWTKLYLLFFL 874 EA + SA A +++ R+G+P+ + D+ FESQ+ ++ ++L ++ R+T H + +G+ ER RTL T I +S+ +W + L L Sbjct: 501 EAYAVPDQSAATTATRLVEDFFCRFGIPEELHSDQGRNFESQVMAEVCKRLGVRKTRTTPLHPQSDGLVERFNRTLATQLAITTSRHQKDWDRHLPLVLL 600 HSP 2 Score: 46.595 bits (109), Expect = 1.127e-15 Identity = 24/92 (26.09%), Postives = 42/92 (45.65%), Query Frame = -2 Query: 892 SFHATHDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKI-TKHTKPPIIHYPTGNRFETLHFDLVGPLP-PDNGFSYLLTMID 1161 + H + H G + L + WP R +V+L+V C C K ++ P+ +G E + D++GP P ++G Y+L +D Sbjct: 403 AIHGPAGVGHFGVTKTLRQLRQRFYWPGCRADVELFVHCCDSCTAKKGPVGRSRAPLQSLRSGAPMERVAVDVLGPFPVTEDGNRYVLVAMD 494
BLAST of SMED30009051 vs. Ensembl Medaka
Match: ENSORLT00000043660.1 (pep primary_assembly:ASM223467v1:15:27996403:27998990:-1 gene:ENSORLG00000029791.1 transcript:ENSORLT00000043660.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.9214 bits (141), Expect = 1.006e-16 Identity = 37/104 (35.58%), Postives = 58/104 (55.77%), Query Frame = -1 Query: 581 RLFEAVTLKSISAEIVANAILQQ*IF-RYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKLYLLFFL 883 R EA L+SI + +A++Q +F R GVP I D+ T F S +QL ++L I+ R+T YH + +G+ ER +TLK + + + +W K +L F L Sbjct: 227 RFPEAFPLRSIKTPKIISALVQ--LFSRVGVPDEIITDQATNFTSGTMKQLQKELGIKGIRTTPYHPQTDGLVERFNQTLKQMLRKFVADTGRDWDK-WLPFVL 327 HSP 2 Score: 47.3654 bits (111), Expect = 1.006e-16 Identity = 28/83 (33.73%), Postives = 40/83 (48.19%), Query Frame = -2 Query: 892 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPLPP-DNGFSYLLTMID 1134 HLG + L S+ WP M +VK + S C CQ K + P+ P G F + D+VGPL +G+ Y+L + D Sbjct: 141 HLGQQKTYMRLGSRFFWPTMFKDVKQYCSTCPDCQVTSHIKVRQAPLHPLPVIGTPFRRIAMDVVGPLEKSSSGYKYILVICD 223
BLAST of SMED30009051 vs. Ensembl Medaka
Match: ENSORLT00000033056.1 (pep primary_assembly:ASM223467v1:23:165526:166795:1 gene:ENSORLG00000025483.1 transcript:ENSORLT00000033056.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 1.700e-16 Identity = 37/104 (35.58%), Postives = 58/104 (55.77%), Query Frame = -1 Query: 581 RLFEAVTLKSISAEIVANAILQQ*IF-RYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKLYLLFFL 883 R EA L+SI + +A++Q +F R GVP I D+ T F S +QL ++L I+ R+T YH + +G+ ER +TLK + + + +W K +L F L Sbjct: 151 RFPEAFPLRSIKTPKIISALVQ--LFSRVGVPDEIITDQATNFTSGTMKQLQKELGIKGIRTTPYHPQTDGLVERFNQTLKQMLRKFVADTGRDWDK-WLPFVL 251 HSP 2 Score: 47.3654 bits (111), Expect = 1.700e-16 Identity = 28/83 (33.73%), Postives = 40/83 (48.19%), Query Frame = -2 Query: 892 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPT-GNRFETLHFDLVGPL-PPDNGFSYLLTMID 1134 HLG + L S+ WP M +VK + S C CQ K + P+ P G F + D+VGPL +G+ Y+L + D Sbjct: 65 HLGQQKTYMRLGSRFFWPTMFKDVKQYCSTCPDCQVTSHIKVRQAPLHPLPVIGTPFRRIAMDVVGPLEKSSSGYKYILVICD 147
BLAST of SMED30009051 vs. Ensembl Medaka
Match: ENSORLT00000041026.1 (pep primary_assembly:ASM223467v1:6:22718848:22725332:1 gene:ENSORLG00000029874.1 transcript:ENSORLT00000041026.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.0106 bits (162), Expect = 2.224e-16 Identity = 37/111 (33.33%), Postives = 70/111 (63.06%), Query Frame = -1 Query: 566 RLFEAVTLKSISAEIVANAILQQ*IFR-YGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKL--YLLFFLVGAAH 883 R EAV L+ I+A ++ A+++ F +G+PK I+ D+ T F+S++F+++ + L I+H S++YH + G ER ++TLK+ + L+S+++W + +L+F GA Sbjct: 811 RFPEAVPLRRITANVITKALVK--FFSVFGLPKVIQTDQGTNFKSKVFKEVLKGLNIRHVTSSSYHPESQGALERFHQTLKSMVRKHCLASRMSWDESLPFLMFAARGAVQ 919 HSP 2 Score: 38.1206 bits (87), Expect = 2.224e-16 Identity = 24/84 (28.57%), Postives = 37/84 (44.05%), Query Frame = -2 Query: 895 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKP--PIIHYPTGNR-FETLHFDLVGPLP-PDNGFSYLLTMI 1134 HLG + + + WP +R +V + C CQ P P++ P FE + D VGPLP G ++LT++ Sbjct: 723 HLGVTKTYDRVLKHFFWPGLRSDVIHYCRTCHICQLAGKPNQVVPPAPLVPIPVHEEPFERVIVDCVGPLPKAKTGNQFILTIM 806
BLAST of SMED30009051 vs. Ensembl Medaka
Match: ENSORLT00000031051.1 (uncharacterized LOC111947296 [Source:NCBI gene;Acc:111947296]) HSP 1 Score: 67.0106 bits (162), Expect = 5.292e-16 Identity = 37/111 (33.33%), Postives = 70/111 (63.06%), Query Frame = -1 Query: 566 RLFEAVTLKSISAEIVANAILQQ*IFR-YGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKT--SRGILSSKVNWTKL--YLLFFLVGAAH 883 R EAV L+ I+A ++ A+++ F +G+PK I+ D+ T F+S++F+++ + L I+H S++YH + G ER ++TLK+ + L+S+++W + +L+F GA Sbjct: 811 RFPEAVPLRRITANVITKALVK--FFSVFGLPKVIQTDQGTNFKSKVFKEVLKGLNIRHVTSSSYHPESQGALERFHQTLKSMVRKHCLASRMSWDESLPFLMFAARGAVQ 919 HSP 2 Score: 36.5798 bits (83), Expect = 5.292e-16 Identity = 23/84 (27.38%), Postives = 37/84 (44.05%), Query Frame = -2 Query: 895 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKP--PIIHYPTGNR-FETLHFDLVGPLP-PDNGFSYLLTMI 1134 HLG + + + WP ++ +V + C CQ P P++ P FE + D VGPLP G ++LT++ Sbjct: 723 HLGVTKTYDRVLKHFFWPGLKSDVIHYCRTCHICQLAGKPNQVVPPAPLVPIPVHEEPFERVIVDCVGPLPKAKTGNQFILTIM 806
BLAST of SMED30009051 vs. Ensembl Medaka
Match: ENSORLT00000038047.1 (pep primary_assembly:ASM223467v1:5:20995901:21001966:-1 gene:ENSORLG00000030265.1 transcript:ENSORLT00000038047.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 55.8398 bits (133), Expect = 6.445e-16 Identity = 30/80 (37.50%), Postives = 46/80 (57.50%), Query Frame = -1 Query: 644 RLFEAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLK 883 R EA L++I+A V A++Q R G+P I D+ T F S+L + ++L I ++T YH + NG+ ER +TLK Sbjct: 748 RFPEAFPLRTITAPAVMRALIQF-FSRVGIPDEILTDQGTNFTSRLMKLFQQQLGITAIKATPYHPQTNGLVERFNQTLK 826 HSP 2 Score: 47.3654 bits (111), Expect = 6.445e-16 Identity = 28/94 (29.79%), Postives = 46/94 (48.94%), Query Frame = -2 Query: 892 HATHDL---HHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIH-YP-TGNRFETLHFDLVGPLP-PDNGFSYLLTMID 1155 H H L HLG + + S+ WP+M +++ + + C CQ+ IT + ++H P F + D+VGPLP +G Y+L + D Sbjct: 651 HLAHTLPWAGHLGRHKTYLRVSSRFFWPSMYTDIQTYCATCPTCQKTAITHKSDRALLHPLPIISTPFRRIAMDIVGPLPKSSSGHEYILVVCD 744
BLAST of SMED30009051 vs. Planmine SMEST
Match: SMESG000015635.1 (SMESG000015635.1) HSP 1 Score: 126.331 bits (316), Expect = 1.512e-33 Identity = 72/93 (77.42%), Postives = 81/93 (87.10%), Query Frame = -2 Query: 253 LIDFDNSPTLDELDTARDHVFSIDSNPQFTTASSYTPYVPKSFKDAKNVNVSNVTDSGFKKRYYGPYKLXXXXXXXXXXXXNGVAQTINL*QL 531 LIDFDNSPTLDELDTARDH SIDSNPQF++ASSYTPYVPKSFKDAK+V + N+ D G K RY GPYKLIDI+DNN+HI ING+ QTINL +L Sbjct: 119 LIDFDNSPTLDELDTARDHFLSIDSNPQFSSASSYTPYVPKSFKDAKHVYIRNIIDLGLKLRYSGPYKLIDIVDNNSHIIINGITQTINLCRL 211
BLAST of SMED30009051 vs. Planmine SMEST
Match: SMESG000000071.1 (SMESG000000071.1) HSP 1 Score: 64.6994 bits (156), Expect = 1.497e-22 Identity = 33/107 (30.84%), Postives = 56/107 (52.34%), Query Frame = -2 Query: 889 KTNFARIFT*ISFHAT-----HDLHHLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKITKHTKPPIIHYPTGNRFETLHFDLVGPLPPDNGFSYLLTMIDK 1194 K N R+ I F T H ++H A + +++++ IWP MR E+K +V C Q +K TK +++P+ FE +H ++VGPL P+N + ++ K Sbjct: 158 KENSPRLLLPIEFRNTCISKVHHINHPRNKATYKIIYNRYIWPNMRKEIKQFVKTCKRYQINKTTKKLLTQKVNFPSRQPFELIHVNIVGPLAPNNNYRQENKLVRK 264 HSP 2 Score: 58.9214 bits (141), Expect = 1.497e-22 Identity = 31/68 (45.59%), Postives = 43/68 (63.24%), Query Frame = -1 Query: 608 IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTLKTSRGILSSKVNW 811 I RYG PK+I D+ QFESQLF+ L + I+ R+TA++ + NG ERL++TLK S + NW Sbjct: 268 IGRYGQPKTIMTDQVRQFESQLFEMLCKIYNIKRNRTTAFNPQANGKIERLHKTLKESLRTKAEDRNW 335 HSP 3 Score: 21.9422 bits (45), Expect = 1.497e-22 Identity = 9/18 (50.00%), Postives = 11/18 (61.11%), Query Frame = -3 Query: 543 PIVLLGWRSTPVRTTGTS 596 P +LLG R+TP T S Sbjct: 340 PTILLGLRNTPNSTDNES 357
BLAST of SMED30009051 vs. Planmine SMEST
Match: SMESG000070163.1 (SMESG000070163.1) HSP 1 Score: 72.7886 bits (177), Expect = 2.578e-15 Identity = 41/85 (48.24%), Postives = 54/85 (63.53%), Query Frame = -1 Query: 2 ISDDLSDNKPRHDLEKVCVDTSSTICNNNPSIK------RNCAYLVTNYKFNENALADVSQPVEIVSKIPHSILSIPDSQRHTNR 238 ++ ++DN+P+ DLEKV +TS+ NNN S+K LVTNY+ +E A+ QPVE VSK+PHSILS PD QR NR Sbjct: 41 VTQTINDNEPQPDLEKVLFETSTITFNNNSSVKTLMDSNEIAPTLVTNYQSDEKIPANALQPVETVSKLPHSILSRPDKQRPVNR 125
BLAST of SMED30009051 vs. Planmine SMEST
Match: SMESG000038549.1 (SMESG000038549.1) HSP 1 Score: 55.8398 bits (133), Expect = 5.108e-15 Identity = 27/76 (35.53%), Postives = 44/76 (57.89%), Query Frame = -1 Query: 647 EAVTLKSISAEIVANAILQQ*IFRYGVPKSIRIDRETQFESQLFQQLTEKLAIQHFRSTAYHLKINGITERLYRTL 874 EA +K A+ +A ++++ RYG PK I D+ T F S++ +++T I R++ YH + NG+TER TL Sbjct: 2648 EAFAMKDQKAKTIAQKLVEEICCRYGTPKQILTDQGTNFMSEIMKEVTNYFKIAKLRTSPYHPQCNGLTERFNGTL 2723 HSP 2 Score: 43.8986 bits (102), Expect = 5.108e-15 Identity = 24/84 (28.57%), Postives = 35/84 (41.67%), Query Frame = -2 Query: 892 HLGFSAAFTLLHSQVIWPAMRHEVKLWVSQCIGCQQHKIT-KHTKPPIIHYPT-GNRFETLHFDLVGPLPPD-NGFSYLLTMID 1134 H+ + + WP M VK W+ C+ C K T K P+ P + D++GPLP NG Y+L + D Sbjct: 2558 HMSVKKTLARMKQKYFWPNMSESVKDWIKNCLTCATRKNTGTKWKAPLKPMPVPAEPMTMISMDILGPLPETLNGNIYILVVTD 2641
BLAST of SMED30009051 vs. Planmine SMEST
Match: SMESG000030612.1 (SMESG000030612.1) HSP 1 Score: 73.9442 bits (180), Expect = 1.141e-13 Identity = 46/89 (51.69%), Postives = 61/89 (68.54%), Query Frame = -2 Query: 235 DELDTARDHVFSIDSNPQFTTASSYTPYVPKSFKDAKNVNVSNVTDSGFKKRYYGPYKLXXXXXXXXXXXXNGVAQTINL*QLKPAL*F 501 D+L+ AR H +I+S+PQFTT + Y PY+P++F+ A +V + DSG K RY GP KL+ I DNNAH I+G QTI+L LK AL F Sbjct: 631 DDLELARAHFLTINSDPQFTTITKYKPYIPRAFEKATHVWIKTTFDSGLKPRYLGPRKLLKITDNNAHAIIDGQNQTISLAHLKLALGF 719 The following BLAST results are available for this feature:
BLAST of SMED30009051 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30009051 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30009051 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009051 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30009051 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30009051 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30009051 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009051 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30009051 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009051 ID=SMED30009051|Name=SMED30009051|organism=Schmidtea mediterranea sexual|type=transcript|length=1396bpback to top Annotated Terms
The following terms have been associated with this transcript:
|