EF-hand calcium binding domain 1

Overview
NameEF-hand calcium binding domain 1
Smed IDSMED30009045
Length (bp)942
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of EF-hand calcium binding domain 1 (SMED30009045) t-SNE clustered cells

Violin plots show distribution of expression levels for EF-hand calcium binding domain 1 (SMED30009045) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of EF-hand calcium binding domain 1 (SMED30009045) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for EF-hand calcium binding domain 1 (SMED30009045) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009045

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30009045h1SMcG0005472 SmedASXL_009438SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30009045h1SMcG0005472 dd_Smed_v6_5488_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30009045h1SMcG0005472 OX_Smed_1.0.16336ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
non-ciliated epidermisSMED30009045h1SMcG0005472 dd_Smed_v4_5488_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Match: EFCAB1 (EF-hand calcium binding domain 1 [Source:HGNC Symbol;Acc:HGNC:25678])

HSP 1 Score: 182.57 bits (462), Expect = 3.574e-56
Identity = 89/173 (51.45%), Postives = 127/173 (73.41%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI----LNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F K E   L+K+    +     +     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG ++ +EW+ GLS+FL GSL+EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDTLTKNCKHFNKFEVNCLIKLFYDLVGGVERQGLVVGLDRNAFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCVNVLEWIHGLSLFLRGSLEEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYELAV 174
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Match: EFCAB1 (EF-hand calcium binding domain 1 [Source:HGNC Symbol;Acc:HGNC:25678])

HSP 1 Score: 143.28 bits (360), Expect = 1.124e-41
Identity = 63/104 (60.58%), Postives = 85/104 (81.73%), Query Frame = 2 
Query:  329 RIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
             +FR FD+DNDG ++ +EW+ GLS+FL GSL+EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:   19 HLFRGFDKDNDGCVNVLEWIHGLSLFLRGSLEEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYELAV 122
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Match: EFCAB1 (EF-hand calcium binding domain 1 [Source:HGNC Symbol;Acc:HGNC:25678])

HSP 1 Score: 143.28 bits (360), Expect = 1.124e-41
Identity = 63/104 (60.58%), Postives = 85/104 (81.73%), Query Frame = 2 
Query:  329 RIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
             +FR FD+DNDG ++ +EW+ GLS+FL GSL+EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:   19 HLFRGFDKDNDGCVNVLEWIHGLSLFLRGSLEEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYELAV 122
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Match: EFCAB1 (EF-hand calcium binding domain 1 [Source:HGNC Symbol;Acc:HGNC:25678])

HSP 1 Score: 122.865 bits (307), Expect = 3.491e-34
Identity = 55/91 (60.44%), Postives = 75/91 (82.42%), Query Frame = 2 
Query:  368 ISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            ++ +EW+ GLS+FL GSL+EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 VNVLEWIHGLSLFLRGSLEEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYELAV 92
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Match: KCNIP4 (potassium voltage-gated channel interacting protein 4 [Source:HGNC Symbol;Acc:HGNC:30083])

HSP 1 Score: 78.5666 bits (192), Expect = 8.100e-17
Identity = 53/173 (30.64%), Postives = 93/173 (53.76%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            M  V +R E  ++ E     + FTK E + L +   F+N + P   ++   F++I  + F   D       +F AFD D++G +S  +++KGLS+ L G+++EK+ +AF +YD+N DG I++EEM  ++K           P   ++  +  V+  F+K D + DG +T + F
Sbjct:    1 MATVRHRPEALELLE---AQSKFTKKELQILYR--GFKN-ECPSGVVNEETFKEIYSQFFPQGDSTTYAHFLFNAFDTDHNGAVSFEDFIKGLSILLRGTVQEKLNWAFNLYDINKDGYITKEEMLDIMKAIYDMMGKCTYPVLKEDAPRQHVETFFQKMDKNKDGVVTIDEF 167
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Match: ncs-1 (Neuronal calcium sensor 1 [Source:UniProtKB/Swiss-Prot;Acc:P36608])

HSP 1 Score: 72.0182 bits (175), Expect = 7.170e-15
Identity = 45/138 (32.61%), Postives = 73/138 (52.90%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFRMTDDM-LLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKP------TLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            P+  L  +GF+ I  + F   D       +F+ FD++ DG I   E+++ LS+   G+L EK+ +AF +YDL+ DG I+R EM  ++        + V+ P EE+  E     VD  F+  D ++D +LT E F+   
Sbjct:   39 PNGMLTEAGFQKIYKQFFPQGDPSDFASFVFKVFDENKDGAIEFHEFIRALSITSRGNLDEKLHWAFKLYDLDQDGFITRNEMLSIVDSIYKMVGSSVQLPEEENTPEKR---VDRIFRMMDKNNDAQLTLEEFKEGA 173
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Match: ncs-3 (Neuronal Calcium Sensor family [Source:UniProtKB/TrEMBL;Acc:Q95ZR9])

HSP 1 Score: 63.929 bits (154), Expect = 6.250e-12
Identity = 51/170 (30.00%), Postives = 88/170 (51.76%), Query Frame = 2 
Query:  134 GNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKP--TLVRQPTEEDPDEGT-KDLVDMTFKKFDVDHDGKLTRENFE 631
             N++  T+  + + + T FTK E +   K    +    P  +L    F+ I  + F   D       +F  FD ++DG IS  E++  LS+   G+L EK+ +AF++YD++ DG I+++EM  ++    +++    E   DE T +  V+  F   D + DG+LTRE F+
Sbjct:   14 ANKKLTTEEVQTLEQVTYFTKKELKKWYKDFVRDC---PSGELKMEEFQGIYKQFFPNGDPSKFAAFVFNVFDDNHDGHISFSEFIAALSITSRGTLDEKLDWAFSLYDVDKDGFITKDEMADIVDAIYSMIGNMLELPKDEDTPQKRVEKIFTNMDRNLDGQLTREEFK 180
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Match: cnb-1 (Protein phosphatase 2B regulatory subunit cnb-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EDN6])

HSP 1 Score: 61.6178 bits (148), Expect = 2.240e-11
Identity = 43/130 (33.08%), Postives = 69/130 (53.08%), Query Frame = 2 
Query:  254 KKLDRSGFRDILHKTF----RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLL-GSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            KKLD  G   +  + F     +  + L+ R+   FD+D +G +   E+++G+S F + G    K+KFAF +YD++ DG IS  E+FQ+LK  +         D   + +VD T    D D DGK++ + F
Sbjct:   28 KKLDVDGSGSLSVEEFMSLPELQQNPLVQRVIDIFDEDGNGEVDFREFIQGISQFSVKGDKNTKLKFAFRIYDMDRDGFISNGELFQVLKMMVGNNL----KDSQLQQIVDKTILFHDKDGDGKISFQEF 153
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Match: cnb-1 (Protein phosphatase 2B regulatory subunit cnb-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EDN6])

HSP 1 Score: 61.2326 bits (147), Expect = 3.126e-11
Identity = 44/134 (32.84%), Postives = 70/134 (52.24%), Query Frame = 2 
Query:  254 KKLDRSGFRDILHKTF----RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLL-GSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            KKLD  G   +  + F     +  + L+ R+   FD+D +G +   E+++G+S F + G    K+KFAF +YD++ DG IS  E+FQ+LK  +         D   + +VD T    D D DGK++ + F   V
Sbjct:   28 KKLDVDGSGSLSVEEFMSLPELQQNPLVQRVIDIFDEDGNGEVDFREFIQGISQFSVKGDKNTKLKFAFRIYDMDRDGFISNGELFQVLKMMVGNNL----KDSQLQQIVDKTILFHDKDGDGKISFQEFCDVV 157
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Match: ncs-2 (Neuronal calcium sensor 2 [Source:UniProtKB/Swiss-Prot;Acc:P36609])

HSP 1 Score: 58.9214 bits (141), Expect = 2.908e-10
Identity = 42/158 (26.58%), Postives = 84/158 (53.16%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTF-RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT--LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E + K+TNFT+ + +   K    ++  K H  L +  F  +    F   + +   + +FR FD DN G I   E++  ++V   G+ ++K+++AF +YD++ +G I  +EM ++++    ++     +  D+  +    M F+K DV++D +LT + F
Sbjct:   16 EFLKKNTNFTEEQIKEWYKGF-VQDCPKGH--LTKEQFIKVYKDFFPSGSAEGFCEHVFRTFDTDNSGFIDFKEFLLAINVTSSGTPEQKLEWAFRMYDIDGNGTIDEKEMIKIIEAIYEMLGPEVTKSADDSPRKRAKMIFEKMDVNNDKELTLKEF 170
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Match: CG2256 (gene:FBgn0029995 transcript:FBtr0301736)

HSP 1 Score: 134.806 bits (338), Expect = 7.825e-37
Identity = 76/185 (41.08%), Postives = 116/185 (62.70%), Query Frame = 2 
Query:  155 KIAERIYKDTNFTKVECENLLKILN--FENSK----------------KPH---KKLDRSGFRDILHKTFRM-TDDMLLDRIFRAFDQDNDGVISPVE-WVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            K+ + + K T FTK E + L +I      N +                KPH   + +DR  FR++LH TF + T+++L++RIF ++D+ ++G+   +E W+ GLS FL G+  E+  F F VYDLN+DG I+++EMF LL+  L++QP +EDPDEG KDLV++  KKFD+D DGK++ E+F   V
Sbjct:  111 KLLDSLRKKTRFTKDELDALCRIYRKLVSNCQYAAKTLASSSSSAAIAKPHAAVEGIDRIVFRELLHSTFDIVTEEILMERIFCSWDKAHEGLPLRLEGWLIGLSTFLRGTPAERAAFCFRVYDLNTDGFITKDEMFTLLRNCLIKQPQDEDPDEGVKDLVEIVLKKFDLDKDGKVSLEDFMGTV 295
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Match: Frq1 (gene:FBgn0030897 transcript:FBtr0335119)

HSP 1 Score: 85.1149 bits (209), Expect = 1.348e-19
Identity = 58/159 (36.48%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +R+  DT FT+ E     K         P+  L   GF  I  + F   D       +FR FD++NDG I   E+++ LSV   G+L EK+++AF +YD+++DG I+REEM+ ++      + +QP  ED +   K  VD  F + D +HDGKLT E F
Sbjct:   14 DRLTTDTYFTEKEIRQWHKGFL---KDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQK-RVDKIFDQMDKNHDGKLTLEEF 168
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Match: Frq1 (gene:FBgn0030897 transcript:FBtr0335120)

HSP 1 Score: 85.1149 bits (209), Expect = 1.348e-19
Identity = 58/159 (36.48%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +R+  DT FT+ E     K         P+  L   GF  I  + F   D       +FR FD++NDG I   E+++ LSV   G+L EK+++AF +YD+++DG I+REEM+ ++      + +QP  ED +   K  VD  F + D +HDGKLT E F
Sbjct:   14 DRLTTDTYFTEKEIRQWHKGFL---KDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQK-RVDKIFDQMDKNHDGKLTLEEF 168
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Match: Frq1 (gene:FBgn0030897 transcript:FBtr0100239)

HSP 1 Score: 85.1149 bits (209), Expect = 1.348e-19
Identity = 58/159 (36.48%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +R+  DT FT+ E     K         P+  L   GF  I  + F   D       +FR FD++NDG I   E+++ LSV   G+L EK+++AF +YD+++DG I+REEM+ ++      + +QP  ED +   K  VD  F + D +HDGKLT E F
Sbjct:   14 DRLTTDTYFTEKEIRQWHKGFL---KDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQK-RVDKIFDQMDKNHDGKLTLEEF 168
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Match: Frq1 (gene:FBgn0030897 transcript:FBtr0074545)

HSP 1 Score: 85.1149 bits (209), Expect = 1.348e-19
Identity = 58/159 (36.48%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +R+  DT FT+ E     K         P+  L   GF  I  + F   D       +FR FD++NDG I   E+++ LSV   G+L EK+++AF +YD+++DG I+REEM+ ++      + +QP  ED +   K  VD  F + D +HDGKLT E F
Sbjct:   14 DRLTTDTYFTEKEIRQWHKGFL---KDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQK-RVDKIFDQMDKNHDGKLTLEEF 168
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Match: efcab1 (EF-hand calcium binding domain 1 [Source:ZFIN;Acc:ZDB-GENE-040914-40])

HSP 1 Score: 186.808 bits (473), Expect = 4.763e-58
Identity = 91/173 (52.60%), Postives = 123/173 (71.10%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKILNFENSKKPHKK----LDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+ I  +AE + +   +F K E E L+++ N    ++  +K    +DR+ FR+ILH TF MTDDM+ DR+ R  D+DNDG +S  EWV+ LSVFL G+L EK+K+ F VYDLN DG ISREEMFQ+LK +L+RQPTEEDPDEG KD+V++  KK D DHDG+++  +FE  V
Sbjct:    8 NRKLIQNLAETLCRQVKHFNKTETECLIRLFNSLLGEQAERKTTIGVDRAKFRNILHHTFGMTDDMMTDRVCRVIDKDNDGYLSVKEWVEALSVFLRGTLDEKMKYCFEVYDLNGDGYISREEMFQMLKDSLIRQPTEEDPDEGIKDIVEIALKKMDYDHDGRVSYADFEKTV 180
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Match: efcab1 (EF-hand calcium binding domain 1 [Source:ZFIN;Acc:ZDB-GENE-040914-40])

HSP 1 Score: 91.2781 bits (225), Expect = 1.521e-22
Identity = 48/105 (45.71%), Postives = 69/105 (65.71%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKILNFENSKKPHKK----LDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIK 436
            NR+ I  +AE + +   +F K E E L+++ N    ++  +K    +DR+ FR+ILH TF MTDDM+ DR+ R  D+DNDG +S  EWV+ LSVFL G+L EK+K
Sbjct:    3 NRKLIQNLAETLCRQVKHFNKTETECLIRLFNSLLGEQAERKTTIGVDRAKFRNILHHTFGMTDDMMTDRVCRVIDKDNDGYLSVKEWVEALSVFLRGTLDEKMK 107
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Match: kcnip3b (Kv channel interacting protein 3b, calsenilin [Source:ZFIN;Acc:ZDB-GENE-040426-1752])

HSP 1 Score: 81.2629 bits (199), Expect = 1.770e-17
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+++AF +YD+N DG I++EEM  ++K     + R  +    D+   + V+  F+K D + DG +T E F
Sbjct:   83 EQLQAQTQFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDATTYAHFLFNAFDMDRNGSIRFEDFVIGLSVLLRGSVTEKLRWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTSPCVKDDAAFEHVEKFFQKMDRNRDGVVTLEEF 238
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Match: kcnip3b (Kv channel interacting protein 3b, calsenilin [Source:ZFIN;Acc:ZDB-GENE-040426-1752])

HSP 1 Score: 81.2629 bits (199), Expect = 1.890e-17
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+++AF +YD+N DG I++EEM  ++K     + R  +    D+   + V+  F+K D + DG +T E F
Sbjct:   90 EQLQAQTQFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDATTYAHFLFNAFDMDRNGSIRFEDFVIGLSVLLRGSVTEKLRWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTSPCVKDDAAFEHVEKFFQKMDRNRDGVVTLEEF 245
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Match: kcnip3b (Kv channel interacting protein 3b, calsenilin [Source:ZFIN;Acc:ZDB-GENE-040426-1752])

HSP 1 Score: 81.2629 bits (199), Expect = 2.202e-17
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+++AF +YD+N DG I++EEM  ++K     + R  +    D+   + V+  F+K D + DG +T E F
Sbjct:   83 EQLQAQTQFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDATTYAHFLFNAFDMDRNGSIRFEDFVIGLSVLLRGSVTEKLRWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTSPCVKDDAAFEHVEKFFQKMDRNRDGVVTLEEF 238
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Match: ptprm (protein tyrosine phosphatase, receptor type M [Source:Xenbase;Acc:XB-GENE-5958903])

HSP 1 Score: 184.882 bits (468), Expect = 3.832e-57
Identity = 91/173 (52.60%), Postives = 126/173 (72.83%), Query Frame = 2 
Query:  137 NRQEITKIAERIYK-DTNFTKVECENLLKILNFENSK----KPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+ + K A+ + +   +F+K E E+L+++ +    +       + +DR+ FR+ILH TF MTDDM++DR+FR FD+DND  IS  EWV+GLSVFL G+L+E+IK+ F VYDLN DG ISREEMF +LK +L++QP+EEDPDEG KDLV++  KK D DHD KL+  +FE AV
Sbjct:    9 NRKNLQKQADALSRLIKHFSKNEVESLIRLYHTLVGRPIDPNTRRGIDRNTFRNILHNTFGMTDDMIMDRVFRGFDKDNDSYISVTEWVEGLSVFLRGTLEERIKYCFGVYDLNGDGYISREEMFHMLKNSLLKQPSEEDPDEGVKDLVEIALKKMDYDHDSKLSYMDFEKAV 181
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Match: KCNIP4 (Kv channel interacting protein 4 [Source:NCBI gene;Acc:100491280])

HSP 1 Score: 80.8777 bits (198), Expect = 2.584e-17
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E +   T FTK E + L +   F+N + P   ++   F+DI  + F   D       +F AFD D++G +S  ++V GLS  L G+++EK+ +AF +YD+N DG I++EEMF ++K           P   +E  +  V+  F+K D++ DG +T E F
Sbjct:   53 ELLEAQTKFTKKELQILYR--GFKN-ECPSGIVNEETFKDIYAQFFPQGDASTYAHFLFNAFDTDHNGSVSFEDFVIGLSTLLRGTIQEKLNWAFNLYDINKDGYITKEEMFDIMKSIYDMMGKCTYPLVREETPRQHVENFFQKMDINKDGVVTIEEF 208
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Match: npm2 (nucleophosmin/nucleoplasmin 2 [Source:Xenbase;Acc:XB-GENE-999546])

HSP 1 Score: 80.1073 bits (196), Expect = 6.317e-17
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDD-MLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPD---EGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++ + T FTK E + L +   F+N + P   ++   F+ I  + F   D  M    +F AFD D+ G +S  ++V GLSV L G++ +K+ +AF +YDLN DG I++EEM  ++K           P+   E  ++ V+  F+K D + DG +T E F
Sbjct:   80 EQLQEQTKFTKKELQVLYR--GFKN-ECPSGIVNEENFKQIYSQFFPQGDSSMYAHFLFNAFDTDHSGSVSFEDFVAGLSVILRGTIDDKLNWAFNLYDLNKDGCITKEEMLDIMKSIYDMMGKYTYPNMREEAPREHVENFFQKMDRNKDGVVTIEEF 235
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Match: txnl4a (thioredoxin like 4A [Source:Xenbase;Acc:XB-GENE-1004157])

HSP 1 Score: 77.7962 bits (190), Expect = 1.662e-16
Identity = 56/169 (33.14%), Postives = 87/169 (51.48%), Query Frame = 2 
Query:  155 KIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDM-LLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEM-------FQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAA 637
            ++ E + + T FT+ E +   K         P  +LD +GF+ I  + F   D       +F  FD++ DG I   E+++ LSV   G+L EK+++AF +YDL++DG I+R EM       +Q++  T V  P EE+  E     VD  F   D + DGKLT + F+  
Sbjct:   11 EVVEELTRKTYFTEKEVQQWYKGFI---KDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNT-VELPEEENTPEKR---VDRIFAMMDKNSDGKLTLQEFQEG 172
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Match: KCNIP3 (potassium voltage-gated channel interacting protein 3 [Source:NCBI gene;Acc:100494097])

HSP 1 Score: 77.7962 bits (190), Expect = 3.439e-16
Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 2 
Query:  182 TNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            T FTK E ++L +   F+N + P   +D   F+ I  + F   D  M    +F AFD D  G I   ++V GLS+ L G++ EK+K+AF +YD+N DG I++EEM  ++K           P   D+   + V+  F+K D + DG +T + F
Sbjct:   60 TKFTKKELQSLYR--GFKN-ECPSGLVDEETFKLIYSQFFPQGDATMYAHFLFNAFDMDRSGAIRFEDFVIGLSILLRGTIHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTYPLLRDDAPIEHVERFFQKMDRNRDGVVTIDEF 209
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Match: Efcab1 (EF-hand calcium binding domain 1 [Source:MGI Symbol;Acc:MGI:1914043])

HSP 1 Score: 179.874 bits (455), Expect = 1.558e-55
Identity = 91/174 (52.30%), Postives = 129/174 (74.14%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI---LNFENSKKPH--KKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F K E + L+ +   L  + +++P     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EW+ GLS+FL G+L EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPGVVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTLDEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFVDYEKAV 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Match: Efcab1 (EF-hand calcium binding domain 1 [Source:MGI Symbol;Acc:MGI:1914043])

HSP 1 Score: 179.874 bits (455), Expect = 2.471e-55
Identity = 91/174 (52.30%), Postives = 129/174 (74.14%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI---LNFENSKKPH--KKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F K E + L+ +   L  + +++P     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EW+ GLS+FL G+L EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPGVVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTLDEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFVDYEKAV 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Match: Efcab1 (EF-hand calcium binding domain 1 [Source:MGI Symbol;Acc:MGI:1914043])

HSP 1 Score: 161.77 bits (408), Expect = 7.558e-49
Identity = 81/156 (51.92%), Postives = 116/156 (74.36%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI---LNFENSKKPH--KKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKK 586
            NR+++ K+ + + K+  +F K E + L+ +   L  + +++P     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EW+ GLS+FL G+L EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK
Sbjct:    2 NRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPGVVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTLDEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKK 157
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Match: 1700109H08Rik (RIKEN cDNA 1700109H08 gene [Source:MGI Symbol;Acc:MGI:1924286])

HSP 1 Score: 140.969 bits (354), Expect = 2.675e-40
Identity = 73/173 (42.20%), Postives = 113/173 (65.32%), Query Frame = 2 
Query:  140 RQEITKIAERIYKDT-NFTKVECENLLKILNFENSKKPHKKLDRSG-----FRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            ++ + K+ E I K   +F K E E L+++  +     P  K+D +G     FR +L   F MT+DML++R+F  FD+D DG ++  EW+KGL+VFL G+ +EK++F F VY LN D  IS+E++F +LK +L +   EE+ +EG KDLV+++ KK D D+DGK++  +FE AV
Sbjct:    3 QRALKKMVESIRKTVKSFKKFEVECLIRLF-YSLVGCPVGKMDNTGLDCNTFRGVLQNIFGMTNDMLMNRVFFVFDKDGDGYVNLEEWIKGLAVFLRGTFEEKMRFCFEVYYLNGDAYISQEKIFDMLKSSLFQHSPEEENEEGVKDLVEISLKKMDYDNDGKISFADFEKAV 174
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Match: Efcab1 (EF-hand calcium binding domain 1 [Source:MGI Symbol;Acc:MGI:1914043])

HSP 1 Score: 91.2781 bits (225), Expect = 1.602e-22
Identity = 49/106 (46.23%), Postives = 74/106 (69.81%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI---LNFENSKKPH--KKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIK 436
            NR+++ K+ + + K+  +F K E + L+ +   L  + +++P     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EW+ GLS+FL G+L EK+K
Sbjct:    2 NRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPGVVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTLDEKMK 107
[Close]
BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Match: sp|Q3KQ77|EFCB1_XENLA (EF-hand calcium-binding domain-containing protein 1 OS=Xenopus laevis OX=8355 GN=efcab1 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 8.175e-58
Identity = 93/173 (53.76%), Postives = 125/173 (72.25%), Query Frame = 2 
Query:  137 NRQEITKIAERIYK-DTNFTKVECENLLKI----LNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+ + K AE + +   +F+K E E+L+++    +         + +DR+ FR+ILH TF MTDDM++DR+FR FD+DND  IS  EWV+GLSVFL G+L+EKIK+ F VYDLN DG ISREEMF +LK +L++QP+EEDPDEG KDLV++  KK D DHD KL+  +FE AV
Sbjct:    2 NRKNLQKQAEALSRLIKHFSKSEVESLIRLYHTLVGRPGDPNTRRGIDRNTFRNILHNTFGMTDDMIMDRVFRGFDKDNDSFISVTEWVEGLSVFLHGTLEEKIKYCFGVYDLNGDGYISREEMFHMLKNSLLKQPSEEDPDEGVKDLVEIALKKMDYDHDSKLSYTDFEKAV 174
[Close]
BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Match: sp|Q32L26|EFCB1_BOVIN (EF-hand calcium-binding domain-containing protein 1 OS=Bos taurus OX=9913 GN=EFCAB1 PE=2 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.606e-55
Identity = 90/174 (51.72%), Postives = 127/174 (72.99%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKILNFENSKKPHKK-----LDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F+K E + L+ +      +   ++     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EWV GLSVFL G+L+EK+K+ F V+DLN D  IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDSLTKNCKHFSKFEVKCLINLFYNLVGEVTERQGVIIGLDRNAFRNILHMTFGMTDDMIMDRVFRGFDKDNDGCISVTEWVYGLSVFLRGTLEEKMKYCFEVFDLNGDSFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYEQAV 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Match: sp|Q9HAE3|EFCB1_HUMAN (EF-hand calcium-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=EFCAB1 PE=2 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.716e-55
Identity = 89/173 (51.45%), Postives = 127/173 (73.41%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI----LNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F K E   L+K+    +     +     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG ++ +EW+ GLS+FL GSL+EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDTLTKNCKHFNKFEVNCLIKLFYDLVGGVERQGLVVGLDRNAFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCVNVLEWIHGLSLFLRGSLEEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFADYELAV 174
[Close]
BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Match: sp|Q9D3N2|EFCB1_MOUSE (EF-hand calcium-binding domain-containing protein 1 OS=Mus musculus OX=10090 GN=Efcab1 PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.728e-54
Identity = 91/174 (52.30%), Postives = 129/174 (74.14%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDT-NFTKVECENLLKI---LNFENSKKPH--KKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            NR+++ K+ + + K+  +F K E + L+ +   L  + +++P     LDR+ FR+ILH TF MTDDM++DR+FR FD+DNDG IS  EW+ GLS+FL G+L EK+K+ F V+DLN DG IS+EEMF +LK +L++QP+EEDPDEG KDLV++T KK D DHDGKL+  ++E AV
Sbjct:    2 NRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPGVVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTLDEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDHDHDGKLSFVDYEKAV 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Match: sp|Q757B7|CANB_ASHGO (Calcineurin subunit B OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CNB1 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 8.909e-19
Identity = 66/168 (39.29%), Postives = 90/168 (53.57%), Query Frame = 2 
Query:  152 TKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFR----MTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKE-KIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            +KI E +  DTNF + E E L         +K   KLDR     I    F     ++ + L  RI   FD DN G +   E++ GLSVF   S KE K++FAF +YD++ DG IS  E+F +LK  +V    E   DE  + +VD T  + DVD DGKL+ E F++AV
Sbjct:    6 SKILEDLLDDTNFDREEIERL---------RKRFMKLDRDSSGSIDKNEFMSIPGVSSNPLAGRIMEVFDADNSGDVDFQEFISGLSVFSGRSDKEEKLRFAFKIYDIDKDGYISNGELFIVLK-IMVGNNLE---DEQLQQVVDRTIMENDVDGDGKLSFEEFKSAV 160
[Close]
BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Match: A0A4Z2D3W2 (EF-hand calcium-binding domain-containing protein isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_004794 PE=4 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 2.347e-66
Identity = 118/212 (55.66%), Postives = 155/212 (73.11%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIFNSISYL 757
            M  +  R+ I ++  ++  + NFT+ ECE LL +    + KKP  KLDR  FR+ILH TF+MTDD+++DRIFR FD D DG ++  EWV GLSVFL G+L+EK KFAF VYDLN+DG I+REEMFQLLK +L++QPT+EDPDEG ++LV++ FK+ D+DHDG+L   +F  AVEN  LLLE LG C P E+   AFL TFQE ++  SISY+
Sbjct:    1 MLEISIRKAINRLTNKLASNVNFTRSECEALLNMFVKFDGKKPFGKLDRMTFREILHHTFKMTDDVMIDRIFRVFDNDFDGGVNAQEWVMGLSVFLRGTLEEKTKFAFDVYDLNTDGRITREEMFQLLKYSLIKQPTDEDPDEGIRELVEIAFKRLDMDHDGRLNLTDFRQAVENNALLLEILGQCFPNEEVTTAFLSTFQEIKVPESISYI 212
[Close]
BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Match: A0A267G942 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008202g5 PE=4 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.694e-64
Identity = 116/206 (56.31%), Postives = 152/206 (73.79%), Query Frame = 2 
Query:  140 RQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIFNSISYL 757
            +  I+KIAE ++  TNFTK ECE+LL +           KLDR+ F+DILH +F MTDD+++D++FRAFD D+D  IS  EW+KG+S FL G+L+EK  FAF  YDLNSDG+ISR+EMFQLLK TL+RQP EEDPDEG +DLV++ FKKFD    G +    F+++V +EPLLL+ LG CLP+EKSA AFL TF+E  +  +ISY+
Sbjct:    6 KANISKIAEALHSQTNFTKKECEHLLHMYKDITGGADDAKLDRNKFKDILHNSFGMTDDLMMDQVFRAFDTDSDSNISQEEWLKGISTFLRGTLEEKTSFAFRAYDLNSDGVISRDEMFQLLKSTLIRQPVEEDPDEGVRDLVEIVFKKFDTQKKGTIDYAQFKSSVVDEPLLLQALGACLPDEKSADAFLCTFEEVHVPTTISYI 211
[Close]
BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Match: A4UUI7 (Amphicalbin OS=Branchiostoma belcheri tsingtauense OX=155462 PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 8.942e-64
Identity = 115/206 (55.83%), Postives = 152/206 (73.79%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKIL-NFENSKKP--HKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQES 730
            M ++  ++ + K+AE  Y+ T+FTK E E LL +  + + SKK     K+DR+ FR++LH TF MTDD+L+DR+FRAFDQDND  +S  EWV+GLSVFL G+++EK+K+ F VYDLNSDG ISREEMF +LK TL +QPTEEDPDEG KDLV++T KK D DHD +L+  +++ +VE EPLLLE  GPCLP  K   AF  TF ++
Sbjct:    1 MAHIVAKKALAKLAETCYRQTHFTKQEVEGLLHVFRDLQTSKKGKDEMKMDRATFREVLHYTFAMTDDILMDRVFRAFDQDNDSYVSAQEWVQGLSVFLRGTMEEKMKYCFEVYDLNSDGYISREEMFSMLKNTLAKQPTEEDPDEGIKDLVEITLKKMDHDHDSRLSYSDYKTSVETEPLLLEVFGPCLPAAKQVHAFTATFSDT 206
[Close]
BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Match: A0A4Z2D3X8 (EF-hand calcium-binding domain-containing protein isoform 3 OS=Schistosoma japonicum OX=6182 GN=EWB00_004794 PE=4 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 1.462e-63
Identity = 117/212 (55.19%), Postives = 154/212 (72.64%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIFNSISYL 757
            M  +  R+ I ++  ++  + NFT+ ECE LL +    + KKP  KLDR  FR+ILH TF+MTDD+++DRIFR FD D DG ++  EWV GLSVFL G+L+EK KFAF VYDLN+DG I+REEMFQLLK +L++ PT+EDPDEG ++LV++ FK+ D+DHDG+L   +F  AVEN  LLLE LG C P E+   AFL TFQE ++  SISY+
Sbjct:    1 MLEISIRKAINRLTNKLASNVNFTRSECEALLNMFVKFDGKKPFGKLDRMTFREILHHTFKMTDDVMIDRIFRVFDNDFDGGVNAQEWVMGLSVFLRGTLEEKTKFAFDVYDLNTDGRITREEMFQLLKYSLIK-PTDEDPDEGIRELVEIAFKRLDMDHDGRLNLTDFRQAVENNALLLEILGQCFPNEEVTTAFLSTFQEIKVPESISYI 211
[Close]
BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Match: C3YSJ7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_118548 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 3.080e-63
Identity = 114/206 (55.34%), Postives = 151/206 (73.30%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKIL-NFENSKKP--HKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQES 730
            M ++  ++ + K+AE  Y+ T+FTK E E LL +  + + SKK     K+DR+ FR++LH TF MTDD+L+DR+FR FDQDND  +S  EWV+GLSVFL G+++EK+K+ F VYDLNSDG ISREEMF +LK TL +QPTEEDPDEG KDLV++T KK D DHD +L+  +++ +VE EPLLLE  GPCLP  K   AF  TF ++
Sbjct:    1 MAHIVAKKALAKLAETCYRSTHFTKQEVEGLLHVFRDLQTSKKGKDEMKMDRATFREVLHYTFAMTDDILMDRVFRGFDQDNDSYVSAQEWVQGLSVFLRGTMEEKMKYCFEVYDLNSDGYISREEMFSMLKNTLAKQPTEEDPDEGIKDLVEITLKKMDHDHDSRLSYSDYKTSVETEPLLLEVFGPCLPAAKQVHAFTATFSDT 206
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Match: efcab1 (EF-hand calcium binding domain 1 [Source:NCBI gene;Acc:103045906])

HSP 1 Score: 188.734 bits (478), Expect = 5.454e-59
Identity = 93/169 (55.03%), Postives = 120/169 (71.01%), Query Frame = 2 
Query:  149 ITKIAERIYKDT-NFTKVECENLLKILN----FENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            I  +AE++ K   +F K E E L+++ N     +  K+    LDR  FR+ILH TF +TDDM++DR+FR+FD DND  IS  EWV+GLSVFL G+L EKIK+ F VYDLN DG ISREEMF +LK +L+RQPTEE+PDEG KDLV++  KK D DHD +L+   FE +V
Sbjct:   12 IQNLAEKLCKQAKHFNKTEAECLIRLFNELLEGQTEKRVGLGLDRGKFRNILHNTFGLTDDMIMDRVFRSFDSDNDSYISVKEWVEGLSVFLRGTLDEKIKYCFEVYDLNGDGYISREEMFHMLKNSLIRQPTEEEPDEGIKDLVEIILKKMDYDHDSRLSYSEFEKSV 180
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Match: ENSAMXT00000041249.1 (calsenilin [Source:NCBI gene;Acc:103024127])

HSP 1 Score: 82.4185 bits (202), Expect = 4.033e-18
Identity = 56/159 (35.22%), Postives = 85/159 (53.46%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++   T FTK E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K     + R       DE   + V+  F+K D + DG +T E F
Sbjct:   54 EQLQAQTKFTKKELQSLYR--GFKN-ECPSGLVDEETFKTIYSQFFPQGDATTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSITEKLNWAFNLYDINKDGYITKEEMLMIMKSIYDMMGRYTYPSVRDEAPSEHVEKFFQKMDRNRDGVVTIEEF 209
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Match: ENSAMXT00000039876.1 (calsenilin [Source:NCBI gene;Acc:103024127])

HSP 1 Score: 81.6481 bits (200), Expect = 3.894e-17
Identity = 56/159 (35.22%), Postives = 85/159 (53.46%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E++   T FTK E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K     + R       DE   + V+  F+K D + DG +T E F
Sbjct:  185 EQLQAQTKFTKKELQSLYR--GFKN-ECPSGLVDEETFKTIYSQFFPQGDATTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSITEKLNWAFNLYDINKDGYITKEEMLMIMKSIYDMMGRYTYPSVRDEAPSEHVEKFFQKMDRNRDGVVTIEEF 340
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Match: kcnip3b (calsenilin-like [Source:NCBI gene;Acc:103026456])

HSP 1 Score: 78.5666 bits (192), Expect = 9.724e-17
Identity = 53/159 (33.33%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E +   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+++AF +YD+N DG I++EEM  ++K     + R  +    ++   + V+  F+K D + DG +T E F
Sbjct:   53 EELQAQTQFTRKELQSLYR--GFKN-ECPSGLVDEETFKTIYSQFFPQGDATTYAHFLFNAFDVDRNGSIRFEDFVIGLSVLLRGSVTEKLRWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTSPSVKEDAPFEHVEKFFQKMDRNRDGVVTIEEF 208
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Match: kcnip3b (calsenilin-like [Source:NCBI gene;Acc:103026456])

HSP 1 Score: 78.1814 bits (191), Expect = 1.813e-16
Identity = 53/159 (33.33%), Postives = 86/159 (54.09%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E +   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+++AF +YD+N DG I++EEM  ++K     + R  +    ++   + V+  F+K D + DG +T E F
Sbjct:   73 EELQAQTQFTRKELQSLYR--GFKN-ECPSGLVDEETFKTIYSQFFPQGDATTYAHFLFNAFDVDRNGSIRFEDFVIGLSVLLRGSVTEKLRWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTSPSVKEDAPFEHVEKFFQKMDRNRDGVVTIEEF 228
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Match: efcab1 (EF-hand calcium binding domain 1 [Source:ZFIN;Acc:ZDB-GENE-040914-40])

HSP 1 Score: 169.859 bits (429), Expect = 8.647e-53
Identity = 95/165 (57.58%), Postives = 122/165 (73.94%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEK-IKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIF 739
            P    +R+ FR ILH  F MTDDM++DR+FRAFD+DNDG IS  EWV+GL++FL G+L+E   K  F VYDLN DG +SREEMF LLK +L++QP+EEDPDEG KDLV++T KK D DHD +L+  +FE +V  + LLLE  GPCLP+ KSA AF     E+++F
Sbjct:    7 PGPAWERNPFRSILHNMFNMTDDMIMDRVFRAFDKDNDGYISSKEWVEGLAIFLRGTLEENTFKNCFEVYDLNGDGYVSREEMFHLLKSSLIKQPSEEDPDEGIKDLVEITLKKMDHDHDSRLSYADFEQSVREDNLLLEAFGPCLPDSKSALAF-----ETQVF 166
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Match: ncs1a (neuronal calcium sensor 1a [Source:ZFIN;Acc:ZDB-GENE-021220-1])

HSP 1 Score: 73.1738 bits (178), Expect = 7.680e-16
Identity = 52/162 (32.10%), Postives = 81/162 (50.00%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTL------VRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            E I + T FT+ E +   +         P+ +LD + F+ I  + F   D       +F  FD++ DG I   E+++ LSV   G++ EK+K+AF +YDL+SDG I+R+EM  ++          V  P EE   E     VD  F   D + DG+L+ + F
Sbjct:   14 EDITRRTYFTEKEVQQWYRGFM---KDCPNGQLDSAAFQTIYRQFFPFGDASKFASFVFNVFDENKDGRIEFGEFIQALSVTSRGTMDEKLKWAFKLYDLDSDGYITRQEMLHIVDAIYKMMGNTVELPEEESTPEKR---VDRIFALMDKNADGRLSLQEF 169
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Match: kcnip2 (Kv channel interacting protein 2 [Source:ZFIN;Acc:ZDB-GENE-081031-91])

HSP 1 Score: 72.7886 bits (177), Expect = 1.696e-15
Identity = 51/163 (31.29%), Postives = 86/163 (52.76%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT---LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            E + + T F + E + L +   F+N + P   +D   F+ I  + F   D       +F AFD+D+ G ++  ++V GLS+ L G+L +K+ +AF +YD+N DG I++EEM  ++K     + R      P    ++ VD  F+K D + DG ++ E F  A 
Sbjct:   39 EILQEQTKFNRKELQILYR--GFKN-ECPTGLVDEEMFKQIYAQFFPQGDASTYAHYLFNAFDKDHSGSLNFEDFVTGLSILLRGTLNDKLNWAFNLYDINKDGYITKEEMNDIIKSIYDMMGRGTYPTVPMSAPEEHVDKFFQKMDQNKDGVVSIEEFMQAC 198
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Match: si:ch211-145b13.5 (si:ch211-145b13.5 [Source:ZFIN;Acc:ZDB-GENE-090313-35])

HSP 1 Score: 72.4034 bits (176), Expect = 2.964e-15
Identity = 48/159 (30.19%), Postives = 87/159 (54.72%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +++   + F++ E + L +  +F+N + P   ++   F++I  + F   D       +F AFD D++G +S  ++V GLSV L G++ EK+ +AF +YD+N DG I++EEM  +++           P   DE   + V+  F+K D + DG +T + F
Sbjct:   54 DKLEAQSKFSRKELQVLYR--SFKN-ECPSGIVNEDNFKEIYAQFFPQGDASTYAHFLFNAFDTDHNGSLSFEDFVMGLSVLLRGTINEKLYWAFNLYDINKDGYITKEEMLDIMRSIYDMMGKYTHPAVQDEAPLEHVEKFFQKMDKNKDGVVTIDEF 209
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Match: VSNL1 (visinin like 1 [Source:HGNC Symbol;Acc:HGNC:12722])

HSP 1 Score: 65.4698 bits (158), Expect = 4.544e-13
Identity = 52/178 (29.21%), Postives = 86/178 (48.31%), Query Frame = 2 
Query:  137 NRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKP------TLVRQPTEED---PDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            N +   ++ E + K T F + E +   K         P  KL+   F+ +  K F   D        FR FD+++DG I   E++  LSV   G+ ++K+ +AF +YDL+ DG ISR EM ++++       T++     ED   P++     VD  F K D ++D  ++ E F+ A 
Sbjct:    5 NSKLAPEVLEDLLKSTEFNEAELKQWYKGFL---KDCPSGKLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNSDGTIDFREFICALSVTSRGNFEQKLNWAFNMYDLDGDGYISRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQR----VDKIFSKMDKNNDCLISLEEFKEAA 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Yeast
Match: CNB1 (Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000001673])

HSP 1 Score: 80.4925 bits (197), Expect = 8.728e-19
Identity = 60/168 (35.71%), Postives = 89/168 (52.98%), Query Frame = 2 
Query:  152 TKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTF----RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFL-LGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            +KI + + +DTNF + E E L         +K   KLDR     I    F     ++ + L  RI   FD DN G +   E++ GLS+F   GS  EK++FAF +YD++ DG IS  E+F +LK  +V    +   DE  + +VD T  + D D DG+L+ E F+ A+
Sbjct:    6 SKIVDGLLEDTNFDRDEIERL---------RKRFMKLDRDSSGSIDKNEFMSIPGVSSNPLAGRIMEVFDADNSGDVDFQEFITGLSIFSGRGSKDEKLRFAFKIYDIDKDGFISNGELFIVLK-IMVGSNLD---DEQLQQIVDRTIVENDSDGDGRLSFEEFKNAI 160
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Yeast
Match: FRQ1 (N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functionally complements the heat sensitivity of a frq1 ts mutant [Source:SGD;Acc:S000002781])

HSP 1 Score: 61.2326 bits (147), Expect = 8.900e-12
Identity = 42/131 (32.06%), Postives = 69/131 (52.67%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFRM-TDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT--LVRQPTEEDPDEGTKDL-VDMTFKKFDVDHDGKLTRENF 628
            P  +L R  F  I  + F   + +   + +F  FD+DN+G I   E++  LS    G+L+EK+ +AF +YDLN DG I+ +EM  ++     ++      + DE T ++ V   FK  D + DG +T + F
Sbjct:   39 PSGQLAREDFVKIYKQFFPFGSPEDFANHLFTVFDKDNNGFIHFEEFITVLSTTSRGTLEEKLSWAFELYDLNHDGYITFDEMLTIVASVYKMMGSMVTLNEDEATPEMRVKKIFKLMDKNEDGYITLDEF 169
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Match: EDO34333 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPM7])

HSP 1 Score: 179.489 bits (454), Expect = 1.213e-55
Identity = 104/221 (47.06%), Postives = 141/221 (63.80%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIFNSISYLGKQDRIVVT 784
            M ++  R+   K+AE I++ T+F+K E  NLL +  F       +K+DR+ FRDILH  F MTDD+L+DR+F+AFD +ND  I   EW+ GLSVFL G+++E+ KF F VYDLNSD  IS+EE+  LLK  +++QPTEEDPDEG ++L D+  K  D DHDG+L+  +F+  V  EPLLLE  G CLP  K    F   F+E       S    Q++ V T
Sbjct:    1 MASIIARKNQEKLAESIFRKTHFSKAEVINLLSL--FSECANQKEKMDRNKFRDILHNKFGMTDDILMDRVFKAFDTNNDSSIDMDEWILGLSVFLRGTVEEQTKFCFDVYDLNSDAFISKEEIMHLLKNAIIKQPTEEDPDEGIRELCDIVLKLMDEDHDGRLSWGDFQKTVVREPLLLEAFGSCLPGLKCVENFSSEFKEKSTAGGPSVYKPQNKTVKT 219
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Match: EDO42747 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0J9])

HSP 1 Score: 80.1073 bits (196), Expect = 1.117e-17
Identity = 50/153 (32.68%), Postives = 84/153 (54.90%), Query Frame = 2 
Query:  182 TNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRI-FRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            T FT++E + + +   F+N + P   +DR  F+ I  + F   D     ++ F  FD + DG +S  ++V GLSV L G++ EK+K+AF +YDL+SDG+I+REE+  ++         E  P   +   ++ V+  F   D + DG +T + F
Sbjct:   66 TKFTRLELQRMYR--GFKN-ECPSGAVDRDTFKRIYAQFFPYGDSAQYAQLLFNVFDHNKDGKVSFEDFVLGLSVSLHGTMDEKLKWAFNLYDLDSDGVITREELATVIHSVHCMMGMESQPTGEESSVQEQVERLFTLMDTNQDGVITEDEF 215
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Match: EDO32744 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU70])

HSP 1 Score: 73.1738 bits (178), Expect = 1.502e-15
Identity = 40/105 (38.10%), Postives = 65/105 (61.90%), Query Frame = 2 
Query:  332 IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKP------TLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +F+ FD++ DG IS  E++  LS+   GSL EK+++AF++YDL+ +G I+R+EM  +++        ++  P +ED  E     VD  FK+ D + DG+LT E F
Sbjct:   70 VFKVFDRNEDGCISFKEFITALSITSRGSLDEKLEWAFSLYDLDKNGYITRDEMLSIVQAIYCMVGNIIDLPRDEDTPEKR---VDKIFKQMDKNEDGRLTMEEF 171
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Match: EDO37926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEC3])

HSP 1 Score: 72.4034 bits (176), Expect = 2.890e-15
Identity = 47/141 (33.33%), Postives = 73/141 (51.77%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFRMTDDM-LLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLL-------------KPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            P   L    F  +  K FR  D   L   +FR FD + DG I   E++  LSV   G++++K+++AF++YD+N DG I+++EMF+++              P L+ Q  E  P+E T  +    F+ FD D DG L+   F
Sbjct:   39 PSGYLTEDQFISMYKKVFRQGDATKLAACVFRRFDVNRDGKIDFREFMCSLSVSTRGTMEQKLRWAFSIYDVNGDGFITKQEMFRIVDALYRTAGDNPDTSPGLISQWDELTPEERTLKV----FRNFDTDRDGVLSLTEF 175
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Match: EDO40012 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8M9])

HSP 1 Score: 72.4034 bits (176), Expect = 3.144e-15
Identity = 38/105 (36.19%), Postives = 62/105 (59.05%), Query Frame = 2 
Query:  332 IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPD--EGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            +FR FD D  G I   E++  LSV   G+L EK+++AF +YD+N+DG ISR EM ++++        ++ P   + + +  D   +K+D+D DG L+   F+A V
Sbjct:   67 VFRTFDDDRSGRIDFKEFMSSLSVTSRGNLDEKLEWAFRIYDINNDGSISRMEMIEIIEAVRKMHACQKWPARVQSSVECTDRIMRKYDIDGDGLLSLGEFKAGV 171
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Match: efcab1 (EF-hand calcium binding domain 1 [Source:NCBI gene;Acc:101154879])

HSP 1 Score: 120.168 bits (300), Expect = 2.554e-32
Identity = 68/182 (37.36%), Postives = 107/182 (58.79%), Query Frame = 2 
Query:  134 GNRQEITKIAERIYKDT-NFTKVECENLLKILNFENSKKPHK-----KLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKD-------LVDMTFKKFDVDHDGKLTRENFEAAV 640
             +++ I  +AE I K   +F K E E L+   N     + H       LDR+ F+ +LH  F MTDD ++DR+FR FD+DNDG ++  EW++GLSV   G+L E+ K+ F +YDL   G I+R+++F LLK +   +  EE  ++  +D       L+++  KK D D DG+L+ ++FE +V
Sbjct:    4 ASKKAIQALAETISKKVEHFNKKEVECLIWKFNALLEAQKHSDGSVSGLDRNQFKVVLHNLFEMTDDFIMDRVFRIFDKDNDGFVTVEEWIQGLSVHFWGTLDEQKKYCFHMYDLKGSGFITRDDIFLLLKDSFHLRLEEEGREDDYQDEQDEINELMEIVLKKMDHDKDGRLSFQDFEKSV 185
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Match: KCNIP3 (calsenilin [Source:NCBI gene;Acc:101175167])

HSP 1 Score: 80.4925 bits (197), Expect = 1.980e-17
Identity = 55/159 (34.59%), Postives = 84/159 (52.83%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K           P   DE   + VD  F+K D + DG +T E F
Sbjct:   56 DQLQAQTKFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDASTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSVTEKLNWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTYPCVRDEAPSEHVDKFFQKMDRNRDGVVTIEEF 211
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Match: KCNIP3 (calsenilin [Source:NCBI gene;Acc:101175167])

HSP 1 Score: 79.7221 bits (195), Expect = 5.019e-17
Identity = 55/159 (34.59%), Postives = 84/159 (52.83%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K           P   DE   + VD  F+K D + DG +T E F
Sbjct:   82 DQLQAQTKFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDASTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSVTEKLNWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTYPCVRDEAPSEHVDKFFQKMDRNRDGVVTIEEF 237
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Match: KCNIP3 (calsenilin [Source:NCBI gene;Acc:101175167])

HSP 1 Score: 80.1073 bits (196), Expect = 5.243e-17
Identity = 55/159 (34.59%), Postives = 84/159 (52.83%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K           P   DE   + VD  F+K D + DG +T E F
Sbjct:   95 DQLQAQTKFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDASTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSVTEKLNWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTYPCVRDEAPSEHVDKFFQKMDRNRDGVVTIEEF 250
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Match: KCNIP3 (calsenilin [Source:NCBI gene;Acc:101175167])

HSP 1 Score: 79.7221 bits (195), Expect = 5.651e-17
Identity = 55/159 (34.59%), Postives = 84/159 (52.83%), Query Frame = 2 
Query:  164 ERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDR-IFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDP---DEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            +++   T FT+ E ++L +   F+N + P   +D   F+ I  + F   D       +F AFD D +G I   ++V GLSV L GS+ EK+ +AF +YD+N DG I++EEM  ++K           P   DE   + VD  F+K D + DG +T E F
Sbjct:   82 DQLQAQTKFTRKELQSLYR--GFKN-ECPSGLVDEETFKSIYSQFFPQGDASTYAHFLFNAFDLDRNGSIRFEDFVIGLSVLLRGSVTEKLNWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRYTYPCVRDEAPSEHVDKFFQKMDRNRDGVVTIEEF 237
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Match: SMESG000017974.1 (SMESG000017974.1)

HSP 1 Score: 415.231 bits (1066), Expect = 4.926e-148
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 2 
Query:  122 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVXXXXXXXXXXXXXXXXXKSAAAFLMTFQESEIFNSISYLGKQDRIVVTSEQ 793
            MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVENEPLLLEGLGPCLPEEKSAAAFLMTFQESEIFNSISYLGKQDRIVVTSEQ
Sbjct:    1 MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRSGFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAVENEPLLLEGLGPCLPEEKSAAAFLMTFQESEIFNSISYLGKQDRIVVTSEQ 224
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Match: SMESG000068889.1 (SMESG000068889.1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.082e-16
Identity = 48/131 (36.64%), Postives = 74/131 (56.49%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFRMTD-DMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT--LVRQPTEEDPDEGTKD-LVDMTFKKFDVDHDGKLTRENF 628
            P   L    F+ I    F   D       +FR FD++NDG I   E++  LSV   GSL++K+++AF++YDL+S+G ISR EM ++++    +V   T+   DE T +   D  F++ D + DGKL+ E F
Sbjct:   39 PTGSLSMEEFKKIYGNFFPYGDASKFAAHVFRTFDKNNDGTIDFREFICALSVTSRGSLEQKLRWAFSMYDLDSNGYISRPEMLEIVQAIYKMVGSVTKLPDDESTPEKRTDKIFRQMDKNLDGKLSLEEF 169
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Match: SMESG000081409.1 (SMESG000081409.1)

HSP 1 Score: 68.5514 bits (166), Expect = 9.913e-14
Identity = 47/137 (34.31%), Postives = 74/137 (54.01%), Query Frame = 2 
Query:  245 KPHKKLDRSGFRDILHKTF----RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLL-GSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            K  KKLD  G   +    F    ++  + L+ R+   FD D++G +   E+++G+S F + G+ + K+KFAF +YD++ DG IS  E+FQ+LK  +     E       + +VD T    D D DGK++ E F A V
Sbjct:   25 KRFKKLDLDGSGSLSVDEFMSLPQLQQNPLVQRVIEIFDSDSNGEVDFKEFIEGMSQFSVKGNKESKLKFAFKIYDIDRDGFISNGELFQVLKMMVGNNLKETQ----LQQIVDKTIMFADKDGDGKISYEEFCAVV 157
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Match: SMESG000019278.1 (SMESG000019278.1)

HSP 1 Score: 63.929 bits (154), Expect = 3.649e-12
Identity = 44/137 (32.12%), Postives = 73/137 (53.28%), Query Frame = 2 
Query:  245 KPHKKLDRSGFRDILHKTF----RMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLL-GSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENFEAAV 640
            K  +KLD  G   +  + F    ++  + L+ R+   FD D +G +   E++ G+S F + G+ + K++FAF +YD++ DG IS  E+FQ+LK  +         D   + +VD T    D D DGK++ E F + V
Sbjct:   25 KRFRKLDLDGSGSLSVEEFMSLPQLQQNPLVQRVIEIFDSDGNGEVDFKEFIDGMSQFSVKGNKEAKLRFAFKIYDMDRDGFISNGELFQVLKIMVGNNL----KDAQLQQIVDKTIIFADKDGDGKISYEEFCSVV 157
[Close]
BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Match: SMESG000014374.1 (SMESG000014374.1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.168e-10
Identity = 39/129 (30.23%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:  248 PHKKLDRSGFRDILHKTFR-MTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSLKEKIKFAFTVYDLNSDGLISREEMFQLLKPT-LVRQPTEEDPDEGTKDLVDMTFKKFDVDHDGKLTRENF 628
            P+ +L++  F D+  + F         + +FR FDQDN G I   E++  +S    G+ +EK+  AF +YD++S+G I   EM +++K   L+   T+    +   D     F+K D + D  L++E F
Sbjct:   95 PNSQLNKKAFLDMYSQYFPDGKAKPFYEHLFRTFDQDNSGNIDFSEFLIAISTTQSGTPEEKLDLAFKLYDIDSNGKIEESEMTEIIKAIYLMVGETQSIAFDSPTDRTKAIFEKMDKNKDNVLSKEEF 223
The following BLAST results are available for this feature:
BLAST of EF-hand calcium binding domain 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
EFCAB13.574e-5651.45EF-hand calcium binding domain 1 [Source:HGNC Symb... [more]
EFCAB11.124e-4160.58EF-hand calcium binding domain 1 [Source:HGNC Symb... [more]
EFCAB11.124e-4160.58EF-hand calcium binding domain 1 [Source:HGNC Symb... [more]
EFCAB13.491e-3460.44EF-hand calcium binding domain 1 [Source:HGNC Symb... [more]
KCNIP48.100e-1730.64potassium voltage-gated channel interacting protei... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ncs-17.170e-1532.61Neuronal calcium sensor 1 [Source:UniProtKB/Swiss... [more]
ncs-36.250e-1230.00Neuronal Calcium Sensor family [Source:UniProtKB/... [more]
cnb-12.240e-1133.08Protein phosphatase 2B regulatory subunit cnb-1 [... [more]
cnb-13.126e-1132.84Protein phosphatase 2B regulatory subunit cnb-1 [... [more]
ncs-22.908e-1026.58Neuronal calcium sensor 2 [Source:UniProtKB/Swiss... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG22567.825e-3741.08gene:FBgn0029995 transcript:FBtr0301736[more]
Frq11.348e-1936.48gene:FBgn0030897 transcript:FBtr0335119[more]
Frq11.348e-1936.48gene:FBgn0030897 transcript:FBtr0335120[more]
Frq11.348e-1936.48gene:FBgn0030897 transcript:FBtr0100239[more]
Frq11.348e-1936.48gene:FBgn0030897 transcript:FBtr0074545[more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
efcab14.763e-5852.60EF-hand calcium binding domain 1 [Source:ZFIN;Acc:... [more]
efcab11.521e-2245.71EF-hand calcium binding domain 1 [Source:ZFIN;Acc:... [more]
kcnip3b1.770e-1733.96Kv channel interacting protein 3b, calsenilin [Sou... [more]
kcnip3b1.890e-1733.96Kv channel interacting protein 3b, calsenilin [Sou... [more]
kcnip3b2.202e-1733.96Kv channel interacting protein 3b, calsenilin [Sou... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ptprm3.832e-5752.60protein tyrosine phosphatase, receptor type M [Sou... [more]
KCNIP42.584e-1733.96Kv channel interacting protein 4 [Source:NCBI gene... [more]
npm26.317e-1733.96nucleophosmin/nucleoplasmin 2 [Source:Xenbase;Acc:... [more]
txnl4a1.662e-1633.14thioredoxin like 4A [Source:Xenbase;Acc:XB-GENE-10... [more]
KCNIP33.439e-1634.64potassium voltage-gated channel interacting protei... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Efcab11.558e-5552.30EF-hand calcium binding domain 1 [Source:MGI Symbo... [more]
Efcab12.471e-5552.30EF-hand calcium binding domain 1 [Source:MGI Symbo... [more]
Efcab17.558e-4951.92EF-hand calcium binding domain 1 [Source:MGI Symbo... [more]
1700109H08Rik2.675e-4042.20RIKEN cDNA 1700109H08 gene [Source:MGI Symbol;Acc:... [more]
Efcab11.602e-2246.23EF-hand calcium binding domain 1 [Source:MGI Symbo... [more]
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BLAST of EF-hand calcium binding domain 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3KQ77|EFCB1_XENLA8.175e-5853.76EF-hand calcium-binding domain-containing protein ... [more]
sp|Q32L26|EFCB1_BOVIN1.606e-5551.72EF-hand calcium-binding domain-containing protein ... [more]
sp|Q9HAE3|EFCB1_HUMAN1.716e-5551.45EF-hand calcium-binding domain-containing protein ... [more]
sp|Q9D3N2|EFCB1_MOUSE1.728e-5452.30EF-hand calcium-binding domain-containing protein ... [more]
sp|Q757B7|CANB_ASHGO8.909e-1939.29Calcineurin subunit B OS=Ashbya gossypii (strain A... [more]
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BLAST of EF-hand calcium binding domain 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2D3W22.347e-6655.66EF-hand calcium-binding domain-containing protein ... [more]
A0A267G9421.694e-6456.31Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A4UUI78.942e-6455.83Amphicalbin OS=Branchiostoma belcheri tsingtauense... [more]
A0A4Z2D3X81.462e-6355.19EF-hand calcium-binding domain-containing protein ... [more]
C3YSJ73.080e-6355.34Uncharacterized protein OS=Branchiostoma floridae ... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
efcab15.454e-5955.03EF-hand calcium binding domain 1 [Source:NCBI gene... [more]
ENSAMXT00000041249.14.033e-1835.22calsenilin [Source:NCBI gene;Acc:103024127][more]
ENSAMXT00000039876.13.894e-1735.22calsenilin [Source:NCBI gene;Acc:103024127][more]
kcnip3b9.724e-1733.33calsenilin-like [Source:NCBI gene;Acc:103026456][more]
kcnip3b1.813e-1633.33calsenilin-like [Source:NCBI gene;Acc:103026456][more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
efcab18.647e-5357.58EF-hand calcium binding domain 1 [Source:ZFIN;Acc:... [more]
ncs1a7.680e-1632.10neuronal calcium sensor 1a [Source:ZFIN;Acc:ZDB-GE... [more]
kcnip21.696e-1531.29Kv channel interacting protein 2 [Source:ZFIN;Acc:... [more]
si:ch211-145b13.52.964e-1530.19si:ch211-145b13.5 [Source:ZFIN;Acc:ZDB-GENE-090313... [more]
VSNL14.544e-1329.21visinin like 1 [Source:HGNC Symbol;Acc:HGNC:12722][more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
CNB18.728e-1935.71Calcineurin B; regulatory subunit of calcineurin, ... [more]
FRQ18.900e-1232.06N-myristoylated calcium-binding protein; may have ... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO343331.213e-5547.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427471.117e-1732.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO327441.502e-1538.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO379262.890e-1533.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO400123.144e-1536.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of EF-hand calcium binding domain 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
efcab12.554e-3237.36EF-hand calcium binding domain 1 [Source:NCBI gene... [more]
KCNIP31.980e-1734.59calsenilin [Source:NCBI gene;Acc:101175167][more]
KCNIP35.019e-1734.59calsenilin [Source:NCBI gene;Acc:101175167][more]
KCNIP35.243e-1734.59calsenilin [Source:NCBI gene;Acc:101175167][more]
KCNIP35.651e-1734.59calsenilin [Source:NCBI gene;Acc:101175167][more]
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BLAST of EF-hand calcium binding domain 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017974.14.926e-148100.00SMESG000017974.1[more]
SMESG000068889.13.082e-1636.64SMESG000068889.1[more]
SMESG000081409.19.913e-1434.31SMESG000081409.1[more]
SMESG000019278.13.649e-1232.12SMESG000019278.1[more]
SMESG000014374.11.168e-1030.23SMESG000014374.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009045 ID=SMED30009045|Name=EF-hand calcium binding domain 1|organism=Schmidtea mediterranea sexual|type=transcript|length=942bp
TTAAAAGCAGGCTAGAATTTGTTATGTTATTCAAAATATTGATTGCTTAA
AGCTGGTGTTTTATTACACTATTTCTTAGTATCTACTAGCAACACTAAAC
TGTCTTTATTTGTCAACCAAAATGCCGAATGTTGGAAATCGTCAAGAAAT
AACAAAAATCGCAGAACGAATTTATAAAGATACTAATTTCACCAAAGTTG
AATGTGAAAACCTCTTAAAAATTCTTAATTTTGAAAATTCCAAAAAACCA
CACAAAAAATTGGATCGAAGTGGATTTCGAGATATCTTACATAAGACATT
TCGCATGACAGATGACATGTTACTCGATAGAATTTTTCGGGCATTTGATC
AGGATAATGATGGAGTTATAAGCCCAGTAGAATGGGTTAAAGGCTTATCA
GTTTTTCTTCTTGGTTCTTTGAAAGAAAAAATTAAATTTGCGTTCACTGT
TTATGATTTGAATTCTGATGGATTAATTTCACGGGAAGAAATGTTTCAAT
TACTCAAGCCAACTCTAGTCCGGCAACCAACTGAGGAGGATCCGGATGAA
GGTACAAAAGATTTAGTTGATATGACTTTCAAGAAATTTGACGTTGATCA
TGATGGAAAGTTAACGAGAGAAAATTTTGAAGCAGCAGTTGAAAATGAGC
CACTGCTTCTAGAAGGTCTTGGACCGTGCTTGCCCGAAGAAAAATCAGCG
GCAGCATTTTTGATGACATTTCAAGAATCTGAGATATTTAACAGCATATC
TTACTTGGGAAAGCAGGATCGAATTGTAGTCACATCAGAACAATGAACAA
TTTTCACGAATATATGAAAAATATTTTTACCAATTTTATCACTTGTATTT
TATCAAAATATTTTTTATTTAAATGGTAGAGAATTTATAATTGCGATGCG
GATATATTTTCATCAAAAGTATTCGTTTGTCTGGTTCTACTG
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protein sequence of SMED30009045-orf-1

>SMED30009045-orf-1 ID=SMED30009045-orf-1|Name=SMED30009045-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=225bp
MPNVGNRQEITKIAERIYKDTNFTKVECENLLKILNFENSKKPHKKLDRS
GFRDILHKTFRMTDDMLLDRIFRAFDQDNDGVISPVEWVKGLSVFLLGSL
KEKIKFAFTVYDLNSDGLISREEMFQLLKPTLVRQPTEEDPDEGTKDLVD
MTFKKFDVDHDGKLTRENFEAAVENEPLLLEGLGPCLPEEKSAAAFLMTF
QESEIFNSISYLGKQDRIVVTSEQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0002063non-ciliated epidermis
Vocabulary: INTERPRO
TermDefinition
IPR028846Recoverin
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00450RECOVERINcoord: 95..114
score: 39.04
coord: 117..135
score: 25.91
coord: 164..184
score: 22.53
coord: 71..92
score: 40.21
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 6..184
e-value: 1.0E-44
score: 154.5
NoneNo IPR availablePANTHERPTHR23055:SF60EF-HAND CALCIUM BINDING DOMAIN 1coord: 6..201
IPR002048EF-hand domainSMARTSM00054efh_1coord: 148..176
e-value: 0.17
score: 21.0
coord: 103..131
e-value: 0.02
score: 24.0
coord: 67..95
e-value: 0.0086
score: 25.3
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 68..93
e-value: 3.9E-6
score: 26.0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 101..173
e-value: 3.6E-11
score: 43.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 63..98
score: 12.728
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 99..134
score: 12.561
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 144..179
score: 11.222
IPR002048EF-hand domainCDDcd00051EFhcoord: 68..129
e-value: 1.45245E-8
score: 47.9277
IPR002048EF-hand domainCDDcd00051EFhcoord: 103..169
e-value: 1.00705E-9
score: 51.0093
IPR028846Recoverin familyPANTHERPTHR23055CALCIUM BINDING PROTEINScoord: 6..201
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 112..124
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 76..88
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 15..189