Midasin

Overview
NameMidasin
Smed IDSMED30009044
Length (bp)330
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Midasin (SMED30009044) t-SNE clustered cells

Violin plots show distribution of expression levels for Midasin (SMED30009044) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Midasin (SMED30009044) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Midasin (SMED30009044) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009044

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
oocyteSMED30009044h1SMcG0006646 Contig36082uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30009044h1SMcG0006646 Contig36082newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30009044h1SMcG0006646 Contig36082uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30009044h1SMcG0006646 Contig36082newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302])

HSP 1 Score: 86.2705 bits (212), Expect = 1.669e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  553 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 641

HSP 2 Score: 45.0542 bits (105), Expect = 6.223e-6
Identity = 25/77 (32.47%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  111 QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNR----KLIVINMNQQSDSSDLLG 329
            +  +++ S+     + + L+TL+  V  +  VLL    GCGKTS V+YL+ +  R    +L+ + +  Q+DS  LLG
Sbjct:  294 EQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLG 370
[Close]
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302])

HSP 1 Score: 86.2705 bits (212), Expect = 1.669e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  553 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 641

HSP 2 Score: 45.0542 bits (105), Expect = 6.223e-6
Identity = 25/77 (32.47%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  111 QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNR----KLIVINMNQQSDSSDLLG 329
            +  +++ S+     + + L+TL+  V  +  VLL    GCGKTS V+YL+ +  R    +L+ + +  Q+DS  LLG
Sbjct:  294 EQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLG 370
[Close]
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302])

HSP 1 Score: 86.2705 bits (212), Expect = 1.871e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  626 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 714

HSP 2 Score: 53.9138 bits (128), Expect = 4.008e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  + L N+KL  ++ +   ++SD LG
Sbjct: 1366 TEGMRRLAMLVGRALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLG 1427

HSP 3 Score: 48.521 bits (114), Expect = 3.273e-7
Identity = 25/61 (40.98%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE++ +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2043 HQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2103

HSP 4 Score: 44.669 bits (104), Expect = 8.472e-6
Identity = 25/77 (32.47%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  111 QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNR----KLIVINMNQQSDSSDLLG 329
            +  +++ S+     + + L+TL+  V  +  VLL    GCGKTS V+YL+ +  R    +L+ + +  Q+DS  LLG
Sbjct:  294 EQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLG 370

HSP 5 Score: 44.2838 bits (103), Expect = 9.800e-6
Identity = 21/52 (40.38%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            L R   +++P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1739 LLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFG 1790
[Close]
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302])

HSP 1 Score: 86.2705 bits (212), Expect = 1.871e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  626 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 714

HSP 2 Score: 53.9138 bits (128), Expect = 4.008e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  + L N+KL  ++ +   ++SD LG
Sbjct: 1366 TEGMRRLAMLVGRALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLG 1427

HSP 3 Score: 48.521 bits (114), Expect = 3.273e-7
Identity = 25/61 (40.98%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE++ +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2043 HQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2103

HSP 4 Score: 44.669 bits (104), Expect = 8.472e-6
Identity = 25/77 (32.47%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  111 QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNR----KLIVINMNQQSDSSDLLG 329
            +  +++ S+     + + L+TL+  V  +  VLL    GCGKTS V+YL+ +  R    +L+ + +  Q+DS  LLG
Sbjct:  294 EQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLG 370

HSP 5 Score: 44.2838 bits (103), Expect = 9.800e-6
Identity = 21/52 (40.38%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            L R   +++P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1739 LLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFG 1790
[Close]
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302])

HSP 1 Score: 86.2705 bits (212), Expect = 1.871e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  626 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 714

HSP 2 Score: 53.9138 bits (128), Expect = 4.008e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  + L N+KL  ++ +   ++SD LG
Sbjct: 1366 TEGMRRLAMLVGRALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLG 1427

HSP 3 Score: 48.521 bits (114), Expect = 3.273e-7
Identity = 25/61 (40.98%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE++ +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2043 HQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2103

HSP 4 Score: 44.669 bits (104), Expect = 8.472e-6
Identity = 25/77 (32.47%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  111 QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNR----KLIVINMNQQSDSSDLLG 329
            +  +++ S+     + + L+TL+  V  +  VLL    GCGKTS V+YL+ +  R    +L+ + +  Q+DS  LLG
Sbjct:  294 EQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLG 370

HSP 5 Score: 44.2838 bits (103), Expect = 9.800e-6
Identity = 21/52 (40.38%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            L R   +++P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1739 LLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFG 1790
[Close]
BLAST of Midasin vs. Ensembl Celegans
Match: F55F10.1 (Midasin [Source:UniProtKB/TrEMBL;Acc:O61851])

HSP 1 Score: 65.4698 bits (158), Expect = 1.348e-13
Identity = 38/101 (37.62%), Postives = 59/101 (58.42%), Query Frame = 3 
Query:   24 RQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLL 326
            RQ ++K D   + V+++GR  L +K   +           TR ++QL+E +  CV   EP+LLV ETG GKTS VQ ++ L+   L V+N++  SDS +L+
Sbjct:  327 RQPEVKYD---EKVVSVGRAHLPRKM--SMIKTGRHRLGHTRDVVQLMERIVVCVSHNEPLLLVGETGVGKTSVVQAVADLIGVTLDVVNVSPTSDSDELI 422

HSP 2 Score: 45.8246 bits (107), Expect = 1.014e-6
Identity = 20/52 (38.46%), Postives = 34/52 (65.38%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            ++R + + +P+LL    GCGK+STV  L++L    +  +N++ Q+D SDL G
Sbjct: 1367 IARGLLISKPILLEGAPGCGKSSTVMALAQLTGNPITRLNLSDQTDLSDLFG 1418
[Close]
BLAST of Midasin vs. Ensembl Fly
Match: CG13185 (gene:FBgn0033661 transcript:FBtr0310323)

HSP 1 Score: 76.2554 bits (186), Expect = 3.537e-17
Identity = 37/66 (56.06%), Postives = 48/66 (72.73%), Query Frame = 3 
Query:  132 SFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +F+ TR    +LE ++ CV   EPVLLV ETG GKTS+VQYL++    KL+V+NMN QSD SDL+G
Sbjct:  724 TFSFTRLASCILERIAVCVTHSEPVLLVGETGVGKTSSVQYLAERTEHKLVVVNMNNQSDVSDLVG 789

HSP 2 Score: 53.1434 bits (126), Expect = 3.374e-9
Identity = 22/62 (35.48%), Postives = 40/62 (64.52%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            TR M ++    ++ ++ +EPVLLV  TGCGKT+  Q L+ + + +L ++N +  ++ +D LG
Sbjct: 1435 TRNMTRMAVVTAKALEFDEPVLLVGPTGCGKTTVCQLLASIADVQLRILNCHMHTEGADFLG 1496
[Close]
BLAST of Midasin vs. Ensembl Zebrafish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138])

HSP 1 Score: 90.5077 bits (223), Expect = 3.855e-22
Identity = 50/89 (56.18%), Postives = 61/89 (68.54%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GR  L +KQ  A + SI   +FA TR    LLE LS CV   EP+LLV ETG GKTSTVQYL+ L   +L V+NMNQQSD++DLLG
Sbjct:  603 VTVGRAMLLRKQSDAVRLSIDRQTFAATRPSSVLLEQLSVCVTKGEPILLVGETGTGKTSTVQYLAGLTGHRLRVVNMNQQSDTADLLG 691

HSP 2 Score: 48.1358 bits (113), Expect = 3.070e-7
Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T   ++ LE L +CV+M   V+LV  T  GK+S V  L+ L   +L V+ MN   D+++LLG
Sbjct: 2028 THHSLRPLEALMKCVEMGWMVILVGPTASGKSSLVHLLALLTGHRLRVMAMNSAMDTTELLG 2089

HSP 3 Score: 47.7506 bits (112), Expect = 4.272e-7
Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T+ M +L   + R +   E VLLV +TGCGKT+  Q  + L  +K   +N +   ++SD LG
Sbjct: 1342 TQGMRRLAVLVGRALRFGESVLLVGDTGCGKTTICQLFAALAGQKFFSLNCHLHMETSDFLG 1403

HSP 4 Score: 45.0542 bits (105), Expect = 3.414e-6
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 3 
Query:  180 RCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            R + ++ P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1727 RALKLQRPILLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFG 1776
[Close]
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293])

HSP 1 Score: 89.3521 bits (220), Expect = 1.503e-21
Identity = 46/88 (52.27%), Postives = 64/88 (72.73%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +++GRVE+ +KQ    +++   +FA TR    L+E L+ CV+  E VLLV ETG GKTSTVQYLS +  ++L V+NMNQQSD++DLLG
Sbjct:  579 VSVGRVEILRKQ---TETVHRITFAATRPSSVLIEQLAVCVNKGESVLLVGETGTGKTSTVQYLSHVAGQRLRVVNMNQQSDTADLLG 663

HSP 2 Score: 53.5286 bits (127), Expect = 5.660e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T+ M +L   + R +   EPVLLV +TGCGKT+  Q  + L ++ L  +N +Q  ++SD LG
Sbjct: 1314 TQGMRRLAVLVGRALQFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLG 1375

HSP 3 Score: 53.1434 bits (126), Expect = 7.287e-9
Identity = 33/102 (32.35%), Postives = 54/102 (52.94%), Query Frame = 3 
Query:   36 IKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRK----LIVINMNQQSDSSDLLG 329
            + GD +  +V+ +  + L KKQ K Q S S++         + L +L+  V  + PVLL    GCGKT+ V+YL+ +  R     ++ + +  Q+DS  LLG
Sbjct:  254 VSGD-LCSSVVPVCGIILPKKQSKGQHSESISQLVLVESTCKNLHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLG 354

HSP 4 Score: 46.9802 bits (110), Expect = 9.574e-7
Identity = 25/58 (43.10%), Postives = 35/58 (60.34%), Query Frame = 3 
Query:  156 MQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +Q LE + +CV M   V+LV     GKTS VQ L+ L   KL ++ MN   D+++LLG
Sbjct: 1981 LQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLG 2038
[Close]
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293])

HSP 1 Score: 88.9669 bits (219), Expect = 1.832e-21
Identity = 46/88 (52.27%), Postives = 64/88 (72.73%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +++GRVE+ +KQ    +++   +FA TR    L+E L+ CV+  E VLLV ETG GKTSTVQYLS +  ++L V+NMNQQSD++DLLG
Sbjct:  579 VSVGRVEILRKQ---TETVHRITFAATRPSSVLIEQLAVCVNKGESVLLVGETGTGKTSTVQYLSHVAGQRLRVVNMNQQSDTADLLG 663

HSP 2 Score: 53.1434 bits (126), Expect = 6.898e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T+ M +L   + R +   EPVLLV +TGCGKT+  Q  + L ++ L  +N +Q  ++SD LG
Sbjct: 1314 TQGMRRLAVLVGRALQFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLG 1375

HSP 3 Score: 52.7582 bits (125), Expect = 1.143e-8
Identity = 33/102 (32.35%), Postives = 54/102 (52.94%), Query Frame = 3 
Query:   36 IKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRK----LIVINMNQQSDSSDLLG 329
            + GD +  +V+ +  + L KKQ K Q S S++         + L +L+  V  + PVLL    GCGKT+ V+YL+ +  R     ++ + +  Q+DS  LLG
Sbjct:  254 VSGD-LCSSVVPVCGIILPKKQSKGQHSESISQLVLVESTCKNLHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLG 354

HSP 4 Score: 46.595 bits (109), Expect = 1.350e-6
Identity = 25/58 (43.10%), Postives = 35/58 (60.34%), Query Frame = 3 
Query:  156 MQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +Q LE + +CV M   V+LV     GKTS VQ L+ L   KL ++ MN   D+++LLG
Sbjct: 1994 LQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLG 2051
[Close]
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293])

HSP 1 Score: 58.9214 bits (141), Expect = 7.221e-11
Identity = 34/72 (47.22%), Postives = 47/72 (65.28%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISVA----SFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLV 269
            +++GRVE+ +KQ    +++ V     +FA TR    L+E L+ CV+  E VLLV ETG GKTSTVQYLS + 
Sbjct:  572 VSVGRVEILRKQ---TETVHVHRERITFAATRPSSVLIEQLAVCVNKGESVLLVGETGTGKTSTVQYLSHVA 640

HSP 2 Score: 53.5286 bits (127), Expect = 6.258e-9
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T+ M +L   + R +   EPVLLV +TGCGKT+  Q  + L ++ L  +N +Q  ++SD LG
Sbjct: 1280 TQGMRRLAVLVGRALQFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLG 1341

HSP 3 Score: 50.447 bits (119), Expect = 7.037e-8
Identity = 32/102 (31.37%), Postives = 52/102 (50.98%), Query Frame = 3 
Query:   36 IKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRK----LIVINMNQQSDSSDLLG 329
            + GD +  +V+ +  + L KKQ K Q S+            + L +L+  V  + PVLL    GCGKT+ V+YL+ +  R     ++ + +  Q+DS  LLG
Sbjct:  247 VSGD-LCSSVVPVCGIILPKKQSKGQHSVKEIQLVLVESTCKNLHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLG 347

HSP 4 Score: 46.9802 bits (110), Expect = 1.155e-6
Identity = 25/58 (43.10%), Postives = 35/58 (60.34%), Query Frame = 3 
Query:  156 MQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +Q LE + +CV M   V+LV     GKTS VQ L+ L   KL ++ MN   D+++LLG
Sbjct: 1960 LQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLG 2017
[Close]
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159])

HSP 1 Score: 83.1889 bits (204), Expect = 1.771e-19
Identity = 46/92 (50.00%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISV----ASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ    + + +     +FA TR    LLE L+ CV   EPVLLV ETG GKTS VQYL+    + L V+NMNQQSD++DLLG
Sbjct:  623 VQVGRVRLLRKQ---SEVVRIQKEKCTFAATRPSSVLLEQLAVCVSQGEPVLLVGETGTGKTSAVQYLAYATGQHLRVVNMNQQSDTADLLG 711

HSP 2 Score: 56.9954 bits (136), Expect = 2.263e-10
Identity = 27/62 (43.55%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  S L N+KL  +N +   ++SD LG
Sbjct: 1362 TESMRRLAVLVGRALEFGEPVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLG 1423

HSP 3 Score: 47.7506 bits (112), Expect = 5.032e-7
Identity = 25/61 (40.98%), Postives = 34/61 (55.74%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE + +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2038 HQSFQSLEPIMKCVQMNWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2098

HSP 4 Score: 44.2838 bits (103), Expect = 7.132e-6
Identity = 21/52 (40.38%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            L R   + +P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1734 LLRAAKLNKPILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFG 1785
[Close]
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159])

HSP 1 Score: 83.1889 bits (204), Expect = 1.806e-19
Identity = 46/92 (50.00%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISV----ASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ    + + +     +FA TR    LLE L+ CV   EPVLLV ETG GKTS VQYL+    + L V+NMNQQSD++DLLG
Sbjct:  623 VQVGRVRLLRKQ---SEVVRIQKEKCTFAATRPSSVLLEQLAVCVSQGEPVLLVGETGTGKTSAVQYLAYATGQHLRVVNMNQQSDTADLLG 711

HSP 2 Score: 56.9954 bits (136), Expect = 2.307e-10
Identity = 27/62 (43.55%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  S L N+KL  +N +   ++SD LG
Sbjct: 1362 TESMRRLAVLVGRALEFGEPVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLG 1423

HSP 3 Score: 47.7506 bits (112), Expect = 4.935e-7
Identity = 25/61 (40.98%), Postives = 34/61 (55.74%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE + +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2038 HQSFQSLEPIMKCVQMNWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2098

HSP 4 Score: 44.2838 bits (103), Expect = 7.343e-6
Identity = 21/52 (40.38%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  174 LSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            L R   + +P+LL    G GKTS V  L+K     L+ IN+++Q+D +DL G
Sbjct: 1734 LLRAAKLNKPILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFG 1785
[Close]
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q9NU22|MDN1_HUMAN (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 8.986e-20
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + +GRV L +KQ +A        +FA TR    L+E L+ CV   EPVLLV ETG GKTST+QYL+ +   +L V+NMNQQSD++DLLG
Sbjct:  626 LQVGRVRLLRKQSEAVHLQREKFTFAATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLG 714

HSP 2 Score: 53.9138 bits (128), Expect = 1.925e-8
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R ++  EPVLLV +TGCGKT+  Q  + L N+KL  ++ +   ++SD LG
Sbjct: 1366 TEGMRRLAMLVGRALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLG 1427

HSP 3 Score: 48.521 bits (114), Expect = 1.572e-6
Identity = 25/61 (40.98%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  147 RQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
             Q  Q LE++ +CV M   V+LV     GKTS VQ L+ L    L ++ MN   D+++LLG
Sbjct: 2043 HQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLG 2103
[Close]
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q12019|MDN1_YEAST (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.935e-18
Identity = 44/92 (47.83%), Postives = 64/92 (69.57%), Query Frame = 3 
Query:   57 DNVINIGRVELKKKQWKAQD-SISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            D+ I IGR  L K++   Q  S++   FA T   ++L+E +S C+ M EPVLLV ETG GKT+ VQ L+K++ +KL VIN++QQ+++ DLLG
Sbjct:  599 DDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLG 690

HSP 2 Score: 63.929 bits (154), Expect = 6.829e-12
Identity = 29/69 (42.03%), Postives = 47/69 (68.12%), Query Frame = 3 
Query:  123 SVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            ++ S   T+ M +L   +S C+  +EPVLLV ETGCGKT+  Q L++ + R+LI +N +Q +++ D+LG
Sbjct: 1337 AIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILG 1405
[Close]
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q869L3|MDN1_DICDI (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 1.083e-17
Identity = 36/65 (55.38%), Postives = 50/65 (76.92%), Query Frame = 3 
Query:  135 FAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            FA T   ++L+E +S  +   EP+LLV ETG GKTS VQY++  +N+KL+V+N+NQQSDSSDL+G
Sbjct:  662 FAHTMNSLRLMEKISISIQFNEPILLVGETGTGKTSVVQYIADQLNQKLVVLNLNQQSDSSDLIG 726

HSP 2 Score: 58.5362 bits (140), Expect = 4.837e-10
Identity = 25/62 (40.32%), Postives = 42/62 (67.74%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            TR M +L   + +C++ +EP+LLV ETGC KT+  Q  S L  +KL ++N +Q ++++D +G
Sbjct: 1414 TRSMKRLFSLVGKCLEHKEPILLVGETGCSKTTICQLYSILNKQKLHILNCHQHTETADFIG 1475
[Close]
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|A0A1P8AUY4|MDN1_ARATH (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 6.222e-17
Identity = 43/96 (44.79%), Postives = 62/96 (64.58%), Query Frame = 3 
Query:   48 PIRD--NVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            PI++   ++ IGRV L   +  + D    + F +TR   +LLE ++R V+  EPVLLV ETG GKT+ VQ L+  + +KL V+N++QQSD  DLLG
Sbjct:  619 PIQEFSGILKIGRVSLPLGETASHDR---SRFVETRTSTRLLEKIARSVEYNEPVLLVGETGTGKTTLVQNLAHWIGQKLTVLNLSQQSDIVDLLG 711

HSP 2 Score: 58.5362 bits (140), Expect = 4.694e-10
Identity = 30/88 (34.09%), Postives = 51/88 (57.95%), Query Frame = 3 
Query:   66 INIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +++ + +L   +    DSI    F  T+ M +L   + R   + EPVLLV +TG GKT+  Q LS +  ++L ++N +Q +++SD LG
Sbjct: 1319 VSLAKDDLYNMELPRLDSIQNRKFTWTQSMRRLFFLIDRSYKLREPVLLVGDTGGGKTTICQILSDVKKKRLHILNCHQYTETSDFLG 1406

HSP 3 Score: 47.3654 bits (111), Expect = 4.802e-6
Identity = 32/100 (32.00%), Postives = 52/100 (52.00%), Query Frame = 3 
Query:   33 KIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEE-PVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +I G+ IR   +     ELK    K      V S+ +T+ ++  L  L+  + ++  PVLL   T  GKTS V+YL+ +   K + IN ++Q+D  + LG
Sbjct: 1022 RISGENIRSQPLQRYLGELKGSSDKF-----VGSYVKTKSVIDHLNHLAHAIFIKRYPVLLQGPTSSGKTSLVKYLAAISGNKFVRINNHEQTDIQEYLG 1116
[Close]
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q8T5T1|MDN1_GIAIN (Midasin OS=Giardia intestinalis OX=5741 GN=MDN1 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.503e-12
Identity = 39/111 (35.14%), Postives = 64/111 (57.66%), Query Frame = 3 
Query:    6 ENILMHRQCKIKGDPIRDNV---INIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            E +++    KIK D   D     +  GRV L    +++ D  S+    +T    +LLE +S  +   EP+LLV  TG GKT+ +Q +++ + +KL V+NM+ Q+D++DLLG
Sbjct:  285 EELMLSTLLKIKVDIRHDYTQKRLVCGRVSLPF--YESPDERSLDDICRTNHTTRLLEIISSAIRNNEPLLLVGPTGIGKTTCLQVIARALGKKLHVVNMSSQTDAADLLG 393
[Close]
BLAST of Midasin vs. TrEMBL
Match: A0A0N8DWL2 (Midasin OS=Daphnia magna OX=35525 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.951e-22
Identity = 51/91 (56.04%), Postives = 68/91 (74.73%), Query Frame = 3 
Query:   57 DNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            D+ + IGRV LK+  +K  D++  ++F+ TR    LLE LS CV  +E VLLV ETG GKTS+VQYL++L+  KL+VIN+NQQSDS+DLLG
Sbjct:  592 DDTVGIGRVSLKR--FKTHDTVVKSTFSWTRNSSVLLERLSACVRQQESVLLVGETGTGKTSSVQYLAQLLGHKLLVINLNQQSDSADLLG 680

HSP 2 Score: 53.9138 bits (128), Expect = 7.128e-6
Identity = 27/62 (43.55%), Postives = 40/62 (64.52%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  + +L   +++ +   EPVLLV +TGCGKTS  QYL+   + +L+ IN +Q S+ SD LG
Sbjct: 1336 TYALRRLAVLVAQSLRFVEPVLLVGDTGCGKTSVCQYLAAQKSCQLMTINCHQYSEGSDFLG 1397
[Close]
BLAST of Midasin vs. TrEMBL
Match: A0A164Y7Z6 (Midasin OS=Daphnia magna OX=35525 GN=APZ42_019486 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.289e-21
Identity = 51/93 (54.84%), Postives = 68/93 (73.12%), Query Frame = 3 
Query:   51 IRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + D+ + IGRV LK+  +K  D++  ++F+ TR    LLE LS CV   E VLLV ETG GKTS+VQYL++L+  KL+VIN+NQQSDS+DLLG
Sbjct:  590 MSDDTVGIGRVSLKR--FKTHDTVVKSTFSWTRNSSVLLERLSACVRQLESVLLVGETGTGKTSSVQYLAQLLGHKLLVINLNQQSDSADLLG 680
[Close]
BLAST of Midasin vs. TrEMBL
Match: A0A443SVH5 (Midasin OS=Leptotrombidium deliense OX=299467 GN=B4U80_01128 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.893e-21
Identity = 51/94 (54.26%), Postives = 65/94 (69.15%), Query Frame = 3 
Query:   51 IRDNVINIGRVELKKK-QWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            I  N + IGR++L KK        +S   FA TRQ + LLE +  CV   EPVLLV ETG GKTS++QYL+ L+N+KL+V+NMNQQSD+ DLLG
Sbjct:  567 INSNSVIIGRIKLNKKCDELVTADVSKPFFAYTRQSLNLLEKIGVCVYHNEPVLLVGETGTGKTSSIQYLAHLLNKKLVVVNMNQQSDTCDLLG 660
[Close]
BLAST of Midasin vs. TrEMBL
Match: A0A4Z2BS81 (Uncharacterized protein (Fragment) OS=Takifugu bimaculatus OX=433685 GN=fugu_017327 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 6.790e-21
Identity = 51/102 (50.00%), Postives = 70/102 (68.63%), Query Frame = 3 
Query:   27 QCKIKGDPIRDNVINIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            Q  + G    ++ +++GR+ L +KQ ++   S+   +FA TR    LLE L+ CV+  EPVLLV ETG GKTSTVQYL+KL   +L V+NMNQQSD++DLLG
Sbjct:  658 QMYLPGITRSESEVSVGRISLGRKQAESVHLSVDSQTFAATRPSAVLLEQLAVCVNRGEPVLLVGETGTGKTSTVQYLAKLTGHRLRVVNMNQQSDTADLLG 759
[Close]
BLAST of Midasin vs. TrEMBL
Match: E9FYV2 (Midasin OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_312101 PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 8.371e-21
Identity = 49/93 (52.69%), Postives = 69/93 (74.19%), Query Frame = 3 
Query:   51 IRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            I ++ + IGRV LK+  +K  D+++ ++F+ TR    LLE L+ CV   E VLLV ETG GKTS+VQYL++L+  +L+VIN+NQQSDS+DLLG
Sbjct:  576 ISEDSVGIGRVSLKR--YKINDTVAKSTFSWTRNSSVLLERLAACVRQLESVLLVGETGTGKTSSVQYLAQLLGHRLLVINLNQQSDSADLLG 666

HSP 2 Score: 55.8398 bits (133), Expect = 1.490e-6
Identity = 26/44 (59.09%), Postives = 32/44 (72.73%), Query Frame = 3 
Query:  198 EPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            EPVLLV +TGCGKTS  QYL+   N +L+ IN +Q S+ SD LG
Sbjct: 1340 EPVLLVGDTGCGKTSVCQYLASQKNCQLMTINCHQYSEGSDFLG 1383
[Close]
BLAST of Midasin vs. Ensembl Cavefish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138])

HSP 1 Score: 85.5001 bits (210), Expect = 2.192e-20
Identity = 51/106 (48.11%), Postives = 65/106 (61.32%), Query Frame = 3 
Query:   21 HRQCKI--KGDPIRDNVINIGRVELKKKQWKA-QDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            H  C++   G  + +  + +GR  L +KQ    +      +FA TR    LLE L+ CV   EPVLLV ETG GKTSTVQYL+ L   KL V+NMNQQSD++DLLG
Sbjct:   27 HHYCQLYQPGIIVTELEVTVGRGMLLRKQCDVVRLETEKQTFAATRPSSVLLEQLAVCVTKGEPVLLVGETGTGKTSTVQYLAGLTGHKLRVVNMNQQSDTADLLG 132

HSP 2 Score: 47.7506 bits (112), Expect = 3.218e-7
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T  M +L   + R +   E VLLV +TGCGKT+  Q  + L  +K   +N +   ++SD LG
Sbjct:  783 TEGMRRLAVLVGRALRFGESVLLVGDTGCGKTTICQLFAALAGQKFFSVNCHLHMETSDFLG 844

HSP 3 Score: 47.3654 bits (111), Expect = 4.701e-7
Identity = 24/62 (38.71%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T Q+++ LE++ +CV M    +LV  T  GK+S V  L+ L   +L V+ MN   D+++LLG
Sbjct: 1472 THQVLRPLESIMKCVKMGWMAILVGPTASGKSSLVHLLALLTGHRLRVMAMNSAMDTTELLG 1533

HSP 4 Score: 45.8246 bits (107), Expect = 1.662e-6
Identity = 21/50 (42.00%), Postives = 31/50 (62.00%), Query Frame = 3 
Query:  180 RCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            R + ++ P+LL    G GKTS V  L+K     LI IN+++Q+D +DL G
Sbjct: 1171 RALKLQRPILLEGSPGVGKTSLVAALAKASGNHLIRINLSEQTDVTDLFG 1220
[Close]
BLAST of Midasin vs. Ensembl Yeast
Match: MDN1 (Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus [Source:SGD;Acc:S000004096])

HSP 1 Score: 82.4185 bits (202), Expect = 2.786e-20
Identity = 44/92 (47.83%), Postives = 64/92 (69.57%), Query Frame = 3 
Query:   57 DNVINIGRVELKKKQWKAQD-SISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            D+ I IGR  L K++   Q  S++   FA T   ++L+E +S C+ M EPVLLV ETG GKT+ VQ L+K++ +KL VIN++QQ+++ DLLG
Sbjct:  599 DDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLG 690

HSP 2 Score: 63.929 bits (154), Expect = 9.835e-14
Identity = 29/69 (42.03%), Postives = 47/69 (68.12%), Query Frame = 3 
Query:  123 SVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            ++ S   T+ M +L   +S C+  +EPVLLV ETGCGKT+  Q L++ + R+LI +N +Q +++ D+LG
Sbjct: 1337 AIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILG 1405

HSP 3 Score: 42.3578 bits (98), Expect = 2.931e-6
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3 
Query:  180 RCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            R + + +P+LL    G GKTS +  L+ +   KL  IN+++Q+D  DL G
Sbjct: 1735 RAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFG 1784

HSP 4 Score: 41.2022 bits (95), Expect = 8.359e-6
Identity = 17/56 (30.36%), Postives = 36/56 (64.29%), Query Frame = 3 
Query:  162 LLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            + E++ + ++   P++LV  +  GKT T+++L+ ++  ++ V +MN   DS D+LG
Sbjct: 2036 VYESVLKAINNNWPLVLVGPSNSGKTETIRFLASILGPRVDVFSMNSDIDSMDILG 2091
[Close]
BLAST of Midasin vs. Ensembl Medaka
Match: mdn1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:101161112])

HSP 1 Score: 85.5001 bits (210), Expect = 1.959e-20
Identity = 46/86 (53.49%), Postives = 62/86 (72.09%), Query Frame = 3 
Query:   72 IGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +GR  L++KQ    +++   +FA TR    LLE L+ CV   EPVLLV ETG GKTSTVQ+L+++ +R L V+NMNQQSD++DLLG
Sbjct:  604 VGRATLQRKQ---AEAVHRETFAATRGSSVLLEQLAVCVAKGEPVLLVGETGTGKTSTVQHLARITHR-LRVVNMNQQSDTADLLG 685

HSP 2 Score: 52.373 bits (124), Expect = 9.132e-9
Identity = 27/62 (43.55%), Postives = 40/62 (64.52%), Query Frame = 3 
Query:  144 TRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            T + ++ LE+L +CV+M    +LV  TG GKTS V+ L+ L  R L V+ MN   D+++LLG
Sbjct: 1990 THKALRPLESLMKCVEMGWMAVLVGPTGSGKTSLVRLLALLTGRHLRVMAMNSSMDTTELLG 2051
[Close]
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1)

HSP 1 Score: 223.402 bits (568), Expect = 6.879e-69
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 3 
Query:    3 IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG
Sbjct:  324 IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 432

HSP 2 Score: 51.9878 bits (123), Expect = 7.595e-9
Identity = 25/70 (35.71%), Postives = 42/70 (60.00%), Query Frame = 3 
Query:  120 ISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +++A+   T    ++   L R +  +EPVLLV  TGCGKT+  Q  + L  ++L+ IN +  +++SD LG
Sbjct: 1108 VNIANIEWTFDFRRIAVLLIRSIQCDEPVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLG 1177

HSP 3 Score: 49.2914 bits (116), Expect = 6.257e-8
Identity = 20/66 (30.30%), Postives = 41/66 (62.12%), Query Frame = 3 
Query:  132 SFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +F  T Q +++++    C++ + P+LL    G GK+  + Y++KL+ ++L  + ++ +SDS  LLG
Sbjct:   33 NFVSTNQTVKIIKLFRFCIEKQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLG 98

HSP 4 Score: 46.2098 bits (108), Expect = 9.877e-7
Identity = 21/45 (46.67%), Postives = 33/45 (73.33%), Query Frame = 3 
Query:  195 EEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            ++P+LL    G GKTS V  L+KLV  K++ IN+++Q+D++DL G
Sbjct: 1490 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFG 1534
[Close]
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1)

HSP 1 Score: 223.402 bits (568), Expect = 7.149e-69
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 3 
Query:    3 IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG
Sbjct:  302 IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 410

HSP 2 Score: 51.9878 bits (123), Expect = 7.374e-9
Identity = 25/70 (35.71%), Postives = 42/70 (60.00%), Query Frame = 3 
Query:  120 ISVASFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +++A+   T    ++   L R +  +EPVLLV  TGCGKT+  Q  + L  ++L+ IN +  +++SD LG
Sbjct: 1086 VNIANIEWTFDFRRIAVLLIRSIQCDEPVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLG 1155

HSP 3 Score: 49.2914 bits (116), Expect = 6.255e-8
Identity = 20/66 (30.30%), Postives = 41/66 (62.12%), Query Frame = 3 
Query:  132 SFAQTRQMMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            +F  T Q +++++    C++ + P+LL    G GK+  + Y++KL+ ++L  + ++ +SDS  LLG
Sbjct:   11 NFVSTNQTVKIIKLFRFCIEKQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLG 76

HSP 4 Score: 46.2098 bits (108), Expect = 9.778e-7
Identity = 21/45 (46.67%), Postives = 33/45 (73.33%), Query Frame = 3 
Query:  195 EEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQQSDSSDLLG 329
            ++P+LL    G GKTS V  L+KLV  K++ IN+++Q+D++DL G
Sbjct: 1468 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFG 1512
The following BLAST results are available for this feature:
BLAST of Midasin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MDN11.669e-2051.69midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:... [more]
MDN11.669e-2051.69midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:... [more]
MDN11.871e-2051.69midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:... [more]
MDN11.871e-2051.69midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:... [more]
MDN11.871e-2051.69midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Midasin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F55F10.11.348e-1337.62Midasin [Source:UniProtKB/TrEMBL;Acc:O61851][more]
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BLAST of Midasin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG131853.537e-1756.06gene:FBgn0033661 transcript:FBtr0310323[more]
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BLAST of Midasin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdn13.855e-2256.18midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041... [more]
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BLAST of Midasin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
MDN11.503e-2152.27midasin AAA ATPase 1 [Source:NCBI gene;Acc:1004862... [more]
MDN11.832e-2152.27midasin AAA ATPase 1 [Source:NCBI gene;Acc:1004862... [more]
MDN17.221e-1147.22midasin AAA ATPase 1 [Source:NCBI gene;Acc:1004862... [more]
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BLAST of Midasin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mdn11.771e-1950.00midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:19... [more]
Mdn11.806e-1950.00midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:19... [more]
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BLAST of Midasin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9NU22|MDN1_HUMAN8.986e-2051.69Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2[more]
sp|Q12019|MDN1_YEAST1.935e-1847.83Midasin OS=Saccharomyces cerevisiae (strain ATCC 2... [more]
sp|Q869L3|MDN1_DICDI1.083e-1755.38Midasin OS=Dictyostelium discoideum OX=44689 GN=md... [more]
sp|A0A1P8AUY4|MDN1_ARATH6.222e-1744.79Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE... [more]
sp|Q8T5T1|MDN1_GIAIN9.503e-1235.14Midasin OS=Giardia intestinalis OX=5741 GN=MDN1 PE... [more]
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BLAST of Midasin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0N8DWL21.951e-2256.04Midasin OS=Daphnia magna OX=35525 PE=3 SV=1[more]
A0A164Y7Z61.289e-2154.84Midasin OS=Daphnia magna OX=35525 GN=APZ42_019486 ... [more]
A0A443SVH51.893e-2154.26Midasin OS=Leptotrombidium deliense OX=299467 GN=B... [more]
A0A4Z2BS816.790e-2150.00Uncharacterized protein (Fragment) OS=Takifugu bim... [more]
E9FYV28.371e-2152.69Midasin OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_312... [more]
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BLAST of Midasin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdn12.192e-2048.11midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041... [more]
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BLAST of Midasin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Midasin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MDN12.786e-2047.83Huge dynein-related AAA-type ATPase (midasin); for... [more]
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BLAST of Midasin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Midasin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdn11.959e-2053.49midasin AAA ATPase 1 [Source:NCBI gene;Acc:1011611... [more]
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BLAST of Midasin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000033713.16.879e-69100.00SMESG000033713.1[more]
SMESG000033713.17.149e-69100.00SMESG000033713.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009044 ID=SMED30009044|Name=Midasin|organism=Schmidtea mediterranea sexual|type=transcript|length=330bp
AGATCGAGAATATCCTTATGCATAGGCAATGCAAAATTAAGGGCGACCCT
ATTAGGGATAATGTCATCAATATTGGACGAGTTGAATTGAAGAAAAAGCA
ATGGAAAGCTCAGGATTCTATCAGTGTTGCTAGTTTTGCCCAAACCCGAC
AGATGATGCAGTTACTAGAAACCTTGTCGCGATGTGTTGATATGGAAGAA
CCGGTCTTGCTAGTCAGTGAAACTGGCTGCGGGAAAACTTCCACTGTGCA
ATATCTCAGTAAATTAGTAAATAGGAAATTGATCGTTATCAATATGAATC
AACAAAGTGACAGTTCAGATTTACTAGGAG
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protein sequence of SMED30009044-orf-1

>SMED30009044-orf-1 ID=SMED30009044-orf-1|Name=SMED30009044-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
IENILMHRQCKIKGDPIRDNVINIGRVELKKKQWKAQDSISVASFAQTRQ
MMQLLETLSRCVDMEEPVLLVSETGCGKTSTVQYLSKLVNRKLIVINMNQ
QSDSSDLLG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000027ribosomal large subunit assembly
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0005524ATP binding
GO:0016887ATPase activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR011704ATPase_dyneun-rel_AAA
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 26..109
e-value: 1.1E-15
score: 59.3
NoneNo IPR availablePFAMPF12775AAA_7coord: 47..105
e-value: 1.9E-6
score: 27.5
NoneNo IPR availablePANTHERPTHR22908MIDASIN-RELATEDcoord: 19..109
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 47..109