SMED30009038

Overview
NameSMED30009038
Smed IDSMED30009038
Length (bp)458
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009038 (SMED30009038) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009038 (SMED30009038) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009038 (SMED30009038) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009038 (SMED30009038) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009038

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30009038 dd_Smed_v4_1067_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30009038 dd_Smed_v4_1067_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30009038 dd_Smed_v4_1067_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009038 dd_Smed_v4_1067_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009038 Contig51741uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009038 Contig51741newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009038 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000534:76575..77075 -2244
Homology
BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 1.161e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  116 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 186          
BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.929 bits (154), Expect = 1.239e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  126 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 196          
BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 2.616e-12
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = -2
Query:  155 KMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 355
            K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  176 KQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 242          
BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 3.319e-12
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = -2
Query:  155 KMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 355
            K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  208 KQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 274          
BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 3.650e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  202 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 272          
BLAST of SMED30009038 vs. Ensembl Celegans
Match: vha-20 (Vacuolar H ATPase [Source:UniProtKB/TrEMBL;Acc:Q9XTY9])

HSP 1 Score: 55.4546 bits (132), Expect = 1.261e-9
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = -2
Query:  155 NQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 307
            + D+PA+F I L  VV L++ +  I  G+ +++P +DSIIYRMTT R KKD
Sbjct:  274 SSDYPAIFAIFLGLVVILVVALIYIVVGMASIDPEKDSIIYRMTTTRMKKD 324          
BLAST of SMED30009038 vs. Ensembl Fly
Match: ATP6AP2 (gene:FBgn0037671 transcript:FBtr0081978)

HSP 1 Score: 67.781 bits (164), Expect = 6.197e-14
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            Y  D+P +FNIILW +V   L++ AI + I  M+PGRDSIIYRMT+ R KKD
Sbjct:  268 YGSDYPVIFNIILWFMVVFGLSLLAICYAIAAMDPGRDSIIYRMTSTRIKKD 319          
BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 64.6994 bits (156), Expect = 2.514e-14
Identity = 29/60 (48.33%), Postives = 42/60 (70.00%), Query Frame = -2
Query:  155 SSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 334
            S   ++  YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:   12 SPYNLAYKYNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 71          
BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 68.1662 bits (165), Expect = 9.892e-14
Identity = 33/72 (45.83%), Postives = 50/72 (69.44%), Query Frame = -2
Query:  155 IIQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 370
            I++S +M S+  S   ++  YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  316 ILESKQM-SNPGSPYNLAYKYNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 386          
BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 65.4698 bits (158), Expect = 9.094e-13
Identity = 28/52 (53.85%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  347 YNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 398          
BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 68.1662 bits (165), Expect = 1.206e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  296 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 347          
BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 68.1662 bits (165), Expect = 1.225e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  295 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 346          
BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.389e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  297 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 348          
BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.396e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  284 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 335          
BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.405e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  300 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 351          
BLAST of SMED30009038 vs. Ensembl Mouse
Match: Atp6ap2 (ATPase, H+ transporting, lysosomal accessory protein 2 [Source:MGI Symbol;Acc:MGI:1917745])

HSP 1 Score: 60.8474 bits (146), Expect = 2.821e-11
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++  +FN++LW ++ L L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  299 YNLEYSVVFNLVLWIMIGLALAVIITSYNIWNMDPGYDSIIYRMTNQKIRID 350          
BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q9VHG4|RENR_DROME (ATPase H(+)-transporting accessory protein 2 OS=Drosophila melanogaster OX=7227 GN=ATP6AP2 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.211e-13
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            Y  D+P +FNIILW +V   L++ AI + I  M+PGRDSIIYRMT+ R KKD
Sbjct:  268 YGSDYPVIFNIILWFMVVFGLSLLAICYAIAAMDPGRDSIIYRMTSTRIKKD 319          
BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|P81134|RENR_BOVIN (Renin receptor OS=Bos taurus OX=9913 GN=ATP6AP2 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 2.721e-12
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++P +FN++LW ++ L LT+    + IWNM+PG DSIIYRMT Q+ + D
Sbjct:  300 YNFEYPVVFNLVLWIMIGLALTLIVTCYNIWNMDPGYDSIIYRMTNQKIRMD 351          
BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q5R563|RENR_PONAB (Renin receptor OS=Pongo abelii OX=9601 GN=ATP6AP2 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.652e-11
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  280 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 350          
BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|O75787|RENR_HUMAN (Renin receptor OS=Homo sapiens OX=9606 GN=ATP6AP2 PE=1 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 2.652e-11
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  280 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 350          
BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q9CYN9|RENR_MOUSE (Renin receptor OS=Mus musculus OX=10090 GN=Atp6ap2 PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 1.973e-10
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = -2
Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++  +FN++LW ++ L L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  299 YNLEYSVVFNLVLWIMIGLALAVIITSYNIWNMDPGYDSIIYRMTNQKIRID 350          
BLAST of SMED30009038 vs. TrEMBL
Match: A0A2T7NB47 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_20930 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.512e-19
Identity = 41/60 (68.33%), Postives = 53/60 (88.33%), Query Frame = -2
Query:  155 SSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 334
            SS+ +  DY+QD+PAMFNIILW +VA I++V+ IA+G+WNM+PGRDSIIYRMT+QR KKD
Sbjct:  310 SSLNLEWDYDQDYPAMFNIILWIMVAWIISVFVIAYGMWNMDPGRDSIIYRMTSQRLKKD 369          
BLAST of SMED30009038 vs. TrEMBL
Match: A0A3S5ASK4 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS17536 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.465e-18
Identity = 34/63 (53.97%), Postives = 48/63 (76.19%), Query Frame = -2
Query:  155 SFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 343
               S++ ++PDY+ ++ A+FNIILW  V LIL ++AI WGIWNM+PGRD IIYR+++ R K D
Sbjct:    3 EIASALNLAPDYDPEYAALFNIILWLCVCLILALWAITWGIWNMDPGRDGIIYRISSARLKSD 65          
BLAST of SMED30009038 vs. TrEMBL
Match: A0A210QKB5 (Renin receptor OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20606 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 7.043e-18
Identity = 35/71 (49.30%), Postives = 55/71 (77.46%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            +++   A+   S + +S DY+QD+P++FNIILW ++ + + ++A+ +G+WNM+PGRDSIIYRMTT R KKD
Sbjct:  288 LKATAEATGASSELNLSKDYDQDYPSVFNIILWFMIIMAIVLFAVCYGMWNMDPGRDSIIYRMTTTRLKKD 358          
BLAST of SMED30009038 vs. TrEMBL
Match: K1PDJ7 (Renin receptor OS=Crassostrea gigas OX=29159 GN=CGI_10003016 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.576e-17
Identity = 31/59 (52.54%), Postives = 50/59 (84.75%), Query Frame = -2
Query:  155 SIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 331
            +I +S DY++++PA+FNIILW ++ + + ++ +++G+WN++PGRDSIIYRMTT R KKD
Sbjct:   43 NINLSKDYDENYPAIFNIILWLMIVMFVVIFGVSYGMWNIDPGRDSIIYRMTTTRLKKD 101          
BLAST of SMED30009038 vs. TrEMBL
Match: V4CR93 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_211795 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.400e-15
Identity = 32/61 (52.46%), Postives = 51/61 (83.61%), Query Frame = -2
Query:  155 ESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 337
            +S++ ++ DY+++FPA+FN++LW ++ L + VY I +G+W M+PGRDSIIYRMT+QR KK+
Sbjct:  302 KSNLNLADDYDENFPAIFNMVLWLMIVLAIAVYVIVYGMWFMDPGRDSIIYRMTSQRLKKE 362          
BLAST of SMED30009038 vs. Ensembl Cavefish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 54.6842 bits (130), Expect = 3.624e-9
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I  +K  S+  S   ++  YN ++  +FNI+LW ++AL L V AIA+ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  260 ILESKQISNPGSPYNLAYKYNYEYAVIFNIVLWLMIALALAVIAIAYNLWNMDPGYDSIIYRMTNQKIRMD 330          
BLAST of SMED30009038 vs. Ensembl Nematostella
Match: EDO34171 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ62])

HSP 1 Score: 70.0922 bits (170), Expect = 6.781e-15
Identity = 29/61 (47.54%), Postives = 45/61 (73.77%), Query Frame = -2
Query:  155 ESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 337
            ++ + ++ +Y   + A+FNI LW +V ++L+VY I+  +W M+PGRDSIIYRMT+QR K D
Sbjct:  444 KNPLNLAYNYTPMYSAIFNIWLWLIVVIVLSVYVISLVLWYMDPGRDSIIYRMTSQRVKTD 504          
BLAST of SMED30009038 vs. Ensembl Medaka
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:NCBI gene;Acc:101164352])

HSP 1 Score: 67.3958 bits (163), Expect = 1.358e-13
Identity = 31/71 (43.66%), Postives = 47/71 (66.20%), Query Frame = -2
Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I  +K  S+  S   ++  YN ++  +FNI+LW ++ L+L V  IA+ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  304 ILESKQISNPGSPYNLAYKYNFEYAVIFNIVLWMMIVLVLAVIVIAYNLWNMDPGYDSIIYRMTNQKIRMD 374          
BLAST of SMED30009038 vs. Planmine SMEST
Match: SMESG000068141.1 (SMESG000068141.1)

HSP 1 Score: 137.887 bits (346), Expect = 2.683e-43
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = -2
Query:  155 MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 352
            MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD
Sbjct:    1 MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 66          
The following BLAST results are available for this feature:
BLAST of SMED30009038 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ATP6AP21.161e-1242.25ATPase H+ transporting accessory protein 2 [Source... [more]
ATP6AP21.239e-1242.25ATPase H+ transporting accessory protein 2 [Source... [more]
ATP6AP22.616e-1243.28ATPase H+ transporting accessory protein 2 [Source... [more]
ATP6AP23.319e-1243.28ATPase H+ transporting accessory protein 2 [Source... [more]
ATP6AP23.650e-1242.25ATPase H+ transporting accessory protein 2 [Source... [more]
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BLAST of SMED30009038 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
vha-201.261e-949.02Vacuolar H ATPase [Source:UniProtKB/TrEMBL;Acc:Q9... [more]
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BLAST of SMED30009038 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ATP6AP26.197e-1457.69gene:FBgn0037671 transcript:FBtr0081978[more]
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BLAST of SMED30009038 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
atp6ap22.514e-1448.33ATPase H+ transporting accessory protein 2 [Source... [more]
atp6ap29.892e-1445.83ATPase H+ transporting accessory protein 2 [Source... [more]
atp6ap29.094e-1353.85ATPase H+ transporting accessory protein 2 [Source... [more]
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BLAST of SMED30009038 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ccdc1481.206e-1355.77coiled-coil domain containing 148 [Source:Xenbase;... [more]
ccdc1481.225e-1355.77coiled-coil domain containing 148 [Source:Xenbase;... [more]
ccdc1481.389e-1355.77coiled-coil domain containing 148 [Source:Xenbase;... [more]
ccdc1481.396e-1355.77coiled-coil domain containing 148 [Source:Xenbase;... [more]
ccdc1481.405e-1355.77coiled-coil domain containing 148 [Source:Xenbase;... [more]
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BLAST of SMED30009038 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Atp6ap22.821e-1148.08ATPase, H+ transporting, lysosomal accessory prote... [more]
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BLAST of SMED30009038 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VHG4|RENR_DROME6.211e-1357.69ATPase H(+)-transporting accessory protein 2 OS=Dr... [more]
sp|P81134|RENR_BOVIN2.721e-1250.00Renin receptor OS=Bos taurus OX=9913 GN=ATP6AP2 PE... [more]
sp|Q5R563|RENR_PONAB2.652e-1142.25Renin receptor OS=Pongo abelii OX=9601 GN=ATP6AP2 ... [more]
sp|O75787|RENR_HUMAN2.652e-1142.25Renin receptor OS=Homo sapiens OX=9606 GN=ATP6AP2 ... [more]
sp|Q9CYN9|RENR_MOUSE1.973e-1048.08Renin receptor OS=Mus musculus OX=10090 GN=Atp6ap2... [more]
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BLAST of SMED30009038 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7NB474.512e-1968.33Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A3S5ASK44.465e-1853.97Uncharacterized protein OS=Protopolystoma xenopodi... [more]
A0A210QKB57.043e-1849.30Renin receptor OS=Mizuhopecten yessoensis OX=6573 ... [more]
K1PDJ72.576e-1752.54Renin receptor OS=Crassostrea gigas OX=29159 GN=CG... [more]
V4CR931.400e-1552.46Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of SMED30009038 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp6ap23.624e-946.48ATPase H+ transporting accessory protein 2 [Source... [more]
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BLAST of SMED30009038 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009038 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009038 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO341716.781e-1547.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30009038 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp6ap21.358e-1343.66ATPase H+ transporting accessory protein 2 [Source... [more]
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BLAST of SMED30009038 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000068141.12.683e-43100.00SMESG000068141.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009038 ID=SMED30009038|Name=SMED30009038|organism=Schmidtea mediterranea sexual|type=transcript|length=458bp
GTTCAATAACAGATTTATTTATCACAGTTATCAGTAGATTAATCTTTAAA
CAGATAAGATCATAATTAAGACTATGAAACAAAAATTTATTAAATCAGTT
TAATCAATAAATACTACATTATAATATTATGCCAAGTAATTCAAAGAAAT
TTCAATCTTTCTTTTGACGTTGAGTTGTCATTCTATATATAATACTATCT
CTTCCGGGATTCATATTCCATATTCCCCATGCAATTGCATATACTGTTAA
TATTAATGCAACTACTGTCCACAATATTATGTTGAACATTGCAGGAAAAT
CTTGGTTATAATCTGGTGAAACACCAATTGAGGATTCAAAGCTTGATGCC
ATTTTATTTGACTGGATAATTAATTTTAATCACTGGTTTATTCCACTAAA
CTTTTTCAAATTTAAATTATTTTATTGACTAATTTATTTAGTTGAAATAG
TTTAGCTG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: molecular function
TermDefinition
GO:0038023signaling receptor activity
GO:0004872receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: INTERPRO
TermDefinition
IPR012493Renin_rcpt
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012493Renin receptor-likePFAMPF07850Renin_rcoord: 7..66
e-value: 8.8E-27
score: 93.6
IPR012493Renin receptor-likePANTHERPTHR13351RENIN RECEPTORcoord: 7..66
NoneNo IPR availableTMHMMTMhelixcoord: 25..47