SMED30009038

Overview


Name	SMED30009038
Smed ID	SMED30009038
Length (bp)	458

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30009038 (SMED30009038) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009038 (SMED30009038) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009038 (SMED30009038) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009038 (SMED30009038) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30009038

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
protonephridia	SMED30009038	dd_Smed_v4_1067_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30009038	dd_Smed_v4_1067_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30009038	dd_Smed_v4_1067_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30009038	dd_Smed_v4_1067_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009038	Contig51741	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009038	Contig51741	newmark_ests	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30009038 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000534:76575..77075 -	2244

Homology

[Close]

BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 1.161e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  116 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 186

[Close]

BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.929 bits (154), Expect = 1.239e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  126 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 196

[Close]

BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 2.616e-12
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = -2

Query:  155 KMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 355
            K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  176 KQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 242

[Close]

BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 3.319e-12
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = -2

Query:  155 KMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 355
            K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  208 KQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 274

[Close]

BLAST of SMED30009038 vs. Ensembl Human
Match: ATP6AP2 (ATPase H+ transporting accessory protein 2 [Source:HGNC Symbol;Acc:HGNC:18305])

HSP 1 Score: 63.5438 bits (153), Expect = 3.650e-12
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  202 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 272

[Close]

BLAST of SMED30009038 vs. Ensembl Celegans
Match: vha-20 (Vacuolar H ATPase [Source:UniProtKB/TrEMBL;Acc:Q9XTY9])

HSP 1 Score: 55.4546 bits (132), Expect = 1.261e-9
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = -2

Query:  155 NQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 307
            + D+PA+F I L  VV L++ +  I  G+ +++P +DSIIYRMTT R KKD
Sbjct:  274 SSDYPAIFAIFLGLVVILVVALIYIVVGMASIDPEKDSIIYRMTTTRMKKD 324

[Close]

BLAST of SMED30009038 vs. Ensembl Fly
Match: ATP6AP2 (gene:FBgn0037671 transcript:FBtr0081978)

HSP 1 Score: 67.781 bits (164), Expect = 6.197e-14
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            Y  D+P +FNIILW +V   L++ AI + I  M+PGRDSIIYRMT+ R KKD
Sbjct:  268 YGSDYPVIFNIILWFMVVFGLSLLAICYAIAAMDPGRDSIIYRMTSTRIKKD 319

[Close]

BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 64.6994 bits (156), Expect = 2.514e-14
Identity = 29/60 (48.33%), Postives = 42/60 (70.00%), Query Frame = -2

Query:  155 SSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 334
            S   ++  YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:   12 SPYNLAYKYNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 71

[Close]

BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 68.1662 bits (165), Expect = 9.892e-14
Identity = 33/72 (45.83%), Postives = 50/72 (69.44%), Query Frame = -2

Query:  155 IIQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 370
            I++S +M S+  S   ++  YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  316 ILESKQM-SNPGSPYNLAYKYNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 386

[Close]

BLAST of SMED30009038 vs. Ensembl Zebrafish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 65.4698 bits (158), Expect = 9.094e-13
Identity = 28/52 (53.85%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNI+LW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  347 YNFDYAVIFNIVLWLMIVLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRLD 398

[Close]

BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 68.1662 bits (165), Expect = 1.206e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  296 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 347

[Close]

BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 68.1662 bits (165), Expect = 1.225e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  295 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 346

[Close]

BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.389e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  297 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 348

[Close]

BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.396e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  284 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 335

[Close]

BLAST of SMED30009038 vs. Ensembl Xenopus
Match: ccdc148 (coiled-coil domain containing 148 [Source:Xenbase;Acc:XB-GENE-6050210])

HSP 1 Score: 67.781 bits (164), Expect = 1.405e-13
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN D+  +FNIILW ++ L L V AI++ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  300 YNFDYSVIFNIILWIMIGLALAVIAISYNLWNMDPGYDSIIYRMTNQKIRMD 351

[Close]

BLAST of SMED30009038 vs. Ensembl Mouse
Match: Atp6ap2 (ATPase, H+ transporting, lysosomal accessory protein 2 [Source:MGI Symbol;Acc:MGI:1917745])

HSP 1 Score: 60.8474 bits (146), Expect = 2.821e-11
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++  +FN++LW ++ L L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  299 YNLEYSVVFNLVLWIMIGLALAVIITSYNIWNMDPGYDSIIYRMTNQKIRID 350

[Close]

BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q9VHG4|RENR_DROME (ATPase H(+)-transporting accessory protein 2 OS=Drosophila melanogaster OX=7227 GN=ATP6AP2 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.211e-13
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            Y  D+P +FNIILW +V   L++ AI + I  M+PGRDSIIYRMT+ R KKD
Sbjct:  268 YGSDYPVIFNIILWFMVVFGLSLLAICYAIAAMDPGRDSIIYRMTSTRIKKD 319

[Close]

BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|P81134|RENR_BOVIN (Renin receptor OS=Bos taurus OX=9913 GN=ATP6AP2 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 2.721e-12
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++P +FN++LW ++ L LT+    + IWNM+PG DSIIYRMT Q+ + D
Sbjct:  300 YNFEYPVVFNLVLWIMIGLALTLIVTCYNIWNMDPGYDSIIYRMTNQKIRMD 351

[Close]

BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q5R563|RENR_PONAB (Renin receptor OS=Pongo abelii OX=9601 GN=ATP6AP2 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.652e-11
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  280 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 350

[Close]

BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|O75787|RENR_HUMAN (Renin receptor OS=Homo sapiens OX=9606 GN=ATP6AP2 PE=1 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 2.652e-11
Identity = 30/71 (42.25%), Postives = 45/71 (63.38%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I   K A +  S   ++  YN ++  +FN++LW ++AL L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  280 ILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD 350

[Close]

BLAST of SMED30009038 vs. UniProt/SwissProt
Match: sp|Q9CYN9|RENR_MOUSE (Renin receptor OS=Mus musculus OX=10090 GN=Atp6ap2 PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 1.973e-10
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = -2

Query:  155 YNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 310
            YN ++  +FN++LW ++ L L V   ++ IWNM+PG DSIIYRMT Q+ + D
Sbjct:  299 YNLEYSVVFNLVLWIMIGLALAVIITSYNIWNMDPGYDSIIYRMTNQKIRID 350

[Close]

BLAST of SMED30009038 vs. TrEMBL
Match: A0A2T7NB47 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_20930 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.512e-19
Identity = 41/60 (68.33%), Postives = 53/60 (88.33%), Query Frame = -2

Query:  155 SSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 334
            SS+ +  DY+QD+PAMFNIILW +VA I++V+ IA+G+WNM+PGRDSIIYRMT+QR KKD
Sbjct:  310 SSLNLEWDYDQDYPAMFNIILWIMVAWIISVFVIAYGMWNMDPGRDSIIYRMTSQRLKKD 369

[Close]

BLAST of SMED30009038 vs. TrEMBL
Match: A0A3S5ASK4 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS17536 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.465e-18
Identity = 34/63 (53.97%), Postives = 48/63 (76.19%), Query Frame = -2

Query:  155 SFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 343
               S++ ++PDY+ ++ A+FNIILW  V LIL ++AI WGIWNM+PGRD IIYR+++ R K D
Sbjct:    3 EIASALNLAPDYDPEYAALFNIILWLCVCLILALWAITWGIWNMDPGRDGIIYRISSARLKSD 65

[Close]

BLAST of SMED30009038 vs. TrEMBL
Match: A0A210QKB5 (Renin receptor OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20606 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 7.043e-18
Identity = 35/71 (49.30%), Postives = 55/71 (77.46%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            +++   A+   S + +S DY+QD+P++FNIILW ++ + + ++A+ +G+WNM+PGRDSIIYRMTT R KKD
Sbjct:  288 LKATAEATGASSELNLSKDYDQDYPSVFNIILWFMIIMAIVLFAVCYGMWNMDPGRDSIIYRMTTTRLKKD 358

[Close]

BLAST of SMED30009038 vs. TrEMBL
Match: K1PDJ7 (Renin receptor OS=Crassostrea gigas OX=29159 GN=CGI_10003016 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.576e-17
Identity = 31/59 (52.54%), Postives = 50/59 (84.75%), Query Frame = -2

Query:  155 SIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 331
            +I +S DY++++PA+FNIILW ++ + + ++ +++G+WN++PGRDSIIYRMTT R KKD
Sbjct:   43 NINLSKDYDENYPAIFNIILWLMIVMFVVIFGVSYGMWNIDPGRDSIIYRMTTTRLKKD 101

[Close]

BLAST of SMED30009038 vs. TrEMBL
Match: V4CR93 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_211795 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.400e-15
Identity = 32/61 (52.46%), Postives = 51/61 (83.61%), Query Frame = -2

Query:  155 ESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 337
            +S++ ++ DY+++FPA+FN++LW ++ L + VY I +G+W M+PGRDSIIYRMT+QR KK+
Sbjct:  302 KSNLNLADDYDENFPAIFNMVLWLMIVLAIAVYVIVYGMWFMDPGRDSIIYRMTSQRLKKE 362

[Close]

BLAST of SMED30009038 vs. Ensembl Cavefish
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1960])

HSP 1 Score: 54.6842 bits (130), Expect = 3.624e-9
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I  +K  S+  S   ++  YN ++  +FNI+LW ++AL L V AIA+ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  260 ILESKQISNPGSPYNLAYKYNYEYAVIFNIVLWLMIALALAVIAIAYNLWNMDPGYDSIIYRMTNQKIRMD 330

[Close]

BLAST of SMED30009038 vs. Ensembl Nematostella
Match: EDO34171 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ62])

HSP 1 Score: 70.0922 bits (170), Expect = 6.781e-15
Identity = 29/61 (47.54%), Postives = 45/61 (73.77%), Query Frame = -2

Query:  155 ESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 337
            ++ + ++ +Y   + A+FNI LW +V ++L+VY I+  +W M+PGRDSIIYRMT+QR K D
Sbjct:  444 KNPLNLAYNYTPMYSAIFNIWLWLIVVIVLSVYVISLVLWYMDPGRDSIIYRMTSQRVKTD 504

[Close]

BLAST of SMED30009038 vs. Ensembl Medaka
Match: atp6ap2 (ATPase H+ transporting accessory protein 2 [Source:NCBI gene;Acc:101164352])

HSP 1 Score: 67.3958 bits (163), Expect = 1.358e-13
Identity = 31/71 (43.66%), Postives = 47/71 (66.20%), Query Frame = -2

Query:  155 IQSNKMASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 367
            I  +K  S+  S   ++  YN ++  +FNI+LW ++ L+L V  IA+ +WNM+PG DSIIYRMT Q+ + D
Sbjct:  304 ILESKQISNPGSPYNLAYKYNFEYAVIFNIVLWMMIVLVLAVIVIAYNLWNMDPGYDSIIYRMTNQKIRMD 374

[Close]

BLAST of SMED30009038 vs. Planmine SMEST
Match: SMESG000068141.1 (SMESG000068141.1)

HSP 1 Score: 137.887 bits (346), Expect = 2.683e-43
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = -2

Query:  155 MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 352
            MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD
Sbjct:    1 MASSFESSIGVSPDYNQDFPAMFNIILWTVVALILTVYAIAWGIWNMNPGRDSIIYRMTTQRQKKD 66

The following BLAST results are available for this feature:

BLAST of SMED30009038 vs. Ensembl Human
BLAST of SMED30009038 vs. Ensembl Celegans
BLAST of SMED30009038 vs. Ensembl Fly
BLAST of SMED30009038 vs. Ensembl Zebrafish
BLAST of SMED30009038 vs. Ensembl Xenopus
BLAST of SMED30009038 vs. Ensembl Mouse
BLAST of SMED30009038 vs. UniProt/SwissProt
BLAST of SMED30009038 vs. TrEMBL
BLAST of SMED30009038 vs. Ensembl Cavefish
BLAST of SMED30009038 vs. Ensembl Sea Lamprey
BLAST of SMED30009038 vs. Ensembl Yeast
BLAST of SMED30009038 vs. Ensembl Nematostella
BLAST of SMED30009038 vs. Ensembl Medaka
BLAST of SMED30009038 vs. Planmine SMEST

BLAST of SMED30009038 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ATP6AP2	1.161e-12	42.25	ATPase H+ transporting accessory protein 2 [Source...	[more]
ATP6AP2	1.239e-12	42.25	ATPase H+ transporting accessory protein 2 [Source...	[more]
ATP6AP2	2.616e-12	43.28	ATPase H+ transporting accessory protein 2 [Source...	[more]
ATP6AP2	3.319e-12	43.28	ATPase H+ transporting accessory protein 2 [Source...	[more]
ATP6AP2	3.650e-12	42.25	ATPase H+ transporting accessory protein 2 [Source...	[more]

BLAST of SMED30009038 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
vha-20	1.261e-9	49.02	Vacuolar H ATPase [Source:UniProtKB/TrEMBL;Acc:Q9...	[more]

BLAST of SMED30009038 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ATP6AP2	6.197e-14	57.69	gene:FBgn0037671 transcript:FBtr0081978	[more]

BLAST of SMED30009038 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
atp6ap2	2.514e-14	48.33	ATPase H+ transporting accessory protein 2 [Source...	[more]
atp6ap2	9.892e-14	45.83	ATPase H+ transporting accessory protein 2 [Source...	[more]
atp6ap2	9.094e-13	53.85	ATPase H+ transporting accessory protein 2 [Source...	[more]

BLAST of SMED30009038 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ccdc148	1.206e-13	55.77	coiled-coil domain containing 148 [Source:Xenbase;...	[more]
ccdc148	1.225e-13	55.77	coiled-coil domain containing 148 [Source:Xenbase;...	[more]
ccdc148	1.389e-13	55.77	coiled-coil domain containing 148 [Source:Xenbase;...	[more]
ccdc148	1.396e-13	55.77	coiled-coil domain containing 148 [Source:Xenbase;...	[more]
ccdc148	1.405e-13	55.77	coiled-coil domain containing 148 [Source:Xenbase;...	[more]

BLAST of SMED30009038 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Atp6ap2	2.821e-11	48.08	ATPase, H+ transporting, lysosomal accessory prote...	[more]

BLAST of SMED30009038 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9VHG4\|RENR_DROME	6.211e-13	57.69	ATPase H(+)-transporting accessory protein 2 OS=Dr...	[more]
sp\|P81134\|RENR_BOVIN	2.721e-12	50.00	Renin receptor OS=Bos taurus OX=9913 GN=ATP6AP2 PE...	[more]
sp\|Q5R563\|RENR_PONAB	2.652e-11	42.25	Renin receptor OS=Pongo abelii OX=9601 GN=ATP6AP2 ...	[more]
sp\|O75787\|RENR_HUMAN	2.652e-11	42.25	Renin receptor OS=Homo sapiens OX=9606 GN=ATP6AP2 ...	[more]
sp\|Q9CYN9\|RENR_MOUSE	1.973e-10	48.08	Renin receptor OS=Mus musculus OX=10090 GN=Atp6ap2...	[more]

BLAST of SMED30009038 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2T7NB47	4.512e-19	68.33	Uncharacterized protein OS=Pomacea canaliculata OX...	[more]
A0A3S5ASK4	4.465e-18	53.97	Uncharacterized protein OS=Protopolystoma xenopodi...	[more]
A0A210QKB5	7.043e-18	49.30	Renin receptor OS=Mizuhopecten yessoensis OX=6573 ...	[more]
K1PDJ7	2.576e-17	52.54	Renin receptor OS=Crassostrea gigas OX=29159 GN=CG...	[more]
V4CR93	1.400e-15	52.46	Uncharacterized protein OS=Lottia gigantea OX=2251...	[more]

BLAST of SMED30009038 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
atp6ap2	3.624e-9	46.48	ATPase H+ transporting accessory protein 2 [Source...	[more]

BLAST of SMED30009038 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30009038 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30009038 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO34171	6.781e-15	47.54	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30009038 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
atp6ap2	1.358e-13	43.66	ATPase H+ transporting accessory protein 2 [Source...	[more]

BLAST of SMED30009038 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000068141.1	2.683e-43	100.00	SMESG000068141.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30009038 ID=SMED30009038|Name=SMED30009038|organism=Schmidtea mediterranea sexual|type=transcript|length=458bp
GTTCAATAACAGATTTATTTATCACAGTTATCAGTAGATTAATCTTTAAA
CAGATAAGATCATAATTAAGACTATGAAACAAAAATTTATTAAATCAGTT
TAATCAATAAATACTACATTATAATATTATGCCAAGTAATTCAAAGAAAT
TTCAATCTTTCTTTTGACGTTGAGTTGTCATTCTATATATAATACTATCT
CTTCCGGGATTCATATTCCATATTCCCCATGCAATTGCATATACTGTTAA
TATTAATGCAACTACTGTCCACAATATTATGTTGAACATTGCAGGAAAAT
CTTGGTTATAATCTGGTGAAACACCAATTGAGGATTCAAAGCTTGATGCC
ATTTTATTTGACTGGATAATTAATTTTAATCACTGGTTTATTCCACTAAA
CTTTTTCAAATTTAAATTATTTTATTGACTAATTTATTTAGTTGAAATAG
TTTAGCTG

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000026	gut
PLANA:0000099	neuron
PLANA:0003116	parenchymal cell

Vocabulary: **molecular function**
Term	Definition
GO:0038023	signaling receptor activity
GO:0004872	receptor activity

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR012493	Renin_rcpt

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012493	Renin receptor-like	PFAM	PF07850	Renin_r	coord: 7..66 e-value: 8.8E-27 score: 93.6
IPR012493	Renin receptor-like	PANTHER	PTHR13351	RENIN RECEPTOR	coord: 7..66
None	No IPR available	TMHMM	TMhelix		coord: 25..47

Main menu

SMED30009038

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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