SMED30009033

Overview
NameSMED30009033
Smed IDSMED30009033
Length (bp)949
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009033 (SMED30009033) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009033 (SMED30009033) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009033 (SMED30009033) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009033 (SMED30009033) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009033

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 70

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30009033h1SMcG0004933 Contig5578newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0004933 Contig5578uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0007332 Contig139uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0007332 Contig139newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012211 Contig139uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012211 Contig139newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig5578newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig5578uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0001702 Contig70uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0001702 Contig70newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig70uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig70newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0004933 Contig5578newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0004933 Contig5578uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0007332 Contig139uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0007332 Contig139newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012211 Contig139uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012211 Contig139newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig5578newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig5578uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0001702 Contig70uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0001702 Contig70newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig70uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009033h1SMcG0012210 Contig70newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
parapharyngeal regionSMED30009033h1SMcG0012210 SmedASXL_004341SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30009033h1SMcG0012210 SmedASXL_004341SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012205 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012211 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30009033h1SMcG0012210 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009033h1SMcG0012210 dd_Smed_v4_8361_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009033h1SMcG0012210 dd_Smed_v4_8361_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009033 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004878:13662..20205 -4540
v31.001626:2321..5594 -3811
Homology
[Close]
BLAST of SMED30009033 vs. Planmine SMEST
Match: SMESG000056845.1 (SMESG000056845.1)

HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 2 
Query:   20 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 844
            MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ
Sbjct:    1 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 275
[Close]
BLAST of SMED30009033 vs. Planmine SMEST
Match: SMESG000056845.1 (SMESG000056845.1)

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 2 
Query:   20 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 844
            MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ
Sbjct:    1 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 275
[Close]
BLAST of SMED30009033 vs. Planmine SMEST
Match: SMESG000056845.1 (SMESG000056845.1)

HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 2 
Query:   20 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 844
            MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ
Sbjct:    1 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQ 275
[Close]
BLAST of SMED30009033 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 533.487 bits (1373), Expect = 0.000e+0
Identity = 277/310 (89.35%), Postives = 287/310 (92.58%), Query Frame = 2 
Query:   20 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQXXXXXXXXXXXXXDKASKFDRQNSATSRTSNDSRD 949
            MANADEFK LLQNGDIVDDC NK H A L Q EAKYYL FSMADRVNAY NKLFQ IS+LQ DEIYKLKSNK KSYIKIIEK E  GE+FIKYLTEDG+ ++  AS FKEIPKKA+ PRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQI QLNEIDRD AFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGI FSIQPK+GNKSFRDVDASEIRLIQKTKSIEKSESKST+K++KFDRQNSATSRTSNDSRD
Sbjct:    1 MANADEFKFLLQNGDIVDDCHNKLHFAILVQAEAKYYLHFSMADRVNAYRNKLFQKISELQPDEIYKLKSNKGKSYIKIIEKNEINGEIFIKYLTEDGQSHYNSASIFKEIPKKAKNPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQINQLNEIDRDHAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGISFSIQPKDGNKSFRDVDASEIRLIQKTKSIEKSESKSTEKSAKFDRQNSATSRTSNDSRD 310
[Close]
BLAST of SMED30009033 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 533.102 bits (1372), Expect = 0.000e+0
Identity = 277/310 (89.35%), Postives = 287/310 (92.58%), Query Frame = 2 
Query:   20 MANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMADRVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYLTEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRDQAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGIYFSIQPKEGNKSFRDVDASEIRLIQXXXXXXXXXXXXXDKASKFDRQNSATSRTSNDSRD 949
            MANADEFK LLQNGDIVDDC NK H A L Q EAKYYL FSMADRVNAY NKLFQ IS+LQ DEIYKLKSNK KSYIKIIEK E  GE+FIKYLTEDG+ ++  AS FKEIPKKA+ PRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQI QLNEIDRD AFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGI FSIQPK+GNKSFRDVDASEIRLIQKTKSIEKSESKST+K++KFDRQNSATSRTSNDSRD
Sbjct:    1 MANADEFKFLLQNGDIVDDCHNKLHFAILVQAEAKYYLHFSMADRVNAYRNKLFQKISELQPDEIYKLKSNKGKSYIKIIEKNEINGEIFIKYLTEDGQSHYNSASIFKEIPKKAKNPRTFPPADNQGARKPSIVIHEANDFVTKPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQINQLNEIDRDHAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKNFTEKGISFSIQPKDGNKSFRDVDASEIRLIQKTKSIEKSESKSTEKSAKFDRQNSATSRTSNDSRD 310
The following BLAST results are available for this feature:
BLAST of SMED30009033 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009033 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000056845.10.000e+0100.00SMESG000056845.1[more]
SMESG000056845.10.000e+0100.00SMESG000056845.1[more]
SMESG000056845.10.000e+0100.00SMESG000056845.1[more]
SMESG000044783.10.000e+089.35SMESG000044783.1[more]
SMESG000044783.10.000e+089.35SMESG000044783.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009033 ID=SMED30009033|Name=SMED30009033|organism=Schmidtea mediterranea sexual|type=transcript|length=949bp
TCTATATAAATCTTATATGATGGCGAATGCAGATGAATTTAAATTACTGT
TGCAAAACGGAGATATTGTTGATGATTGTGATAACAAACCTCACGTTGCT
AAATTGGGTCAAGTAGAAGCGAAATATTATTTGGATTTCAGTATGGCAGA
TAGAGTGAATGCTTACCACAATAAATTATTTCAAAACATATCAGATCTTC
AACTTGATGAAATATATAAATTAAAATCTAACAAAGTGAAAAGTTATATC
AAGATTATAGAAAAATACGAATACAAGGGTGAACTTTTTATAAAGTATCT
CACCGAGGATGGACGAATTAATTTCAAACCGGCTTCATTCTTTAAAGAAA
TTCCAAAGAAAGCAAGAAAACCAAGGACATTTCCACCTGCTGATAATCAA
GGTGCGAGAAAGCCAAGCATTGTAATACATGAAGCAAATGATTTCGTGAC
GAAGCCACGATATTACATAATCACTGATGCAAATTGTGAGGAAATCGGAT
CGATAATTCTCATACACAACAGGGCTACTAAAATGCTGAATACGGATAAA
ATGATTAGCAAAACTTATCAAATCAAGCAATTAAATGAAATTGATCGAGA
TCAAGCATTTTATTTGTCCAAATTTCCAGCCAATGAAAGCATTAATGAAT
GGAAATGTGAAACATTTGAGCAAATAAAAGCTTTGGAAATTGGACAAGAA
TGCCGCTACAAAAATGACAGAGAAAATATATATAAAATTACAGCTAAAAA
TTTTACAGAAAAAGGAATTTATTTTTCTATACAGCCAAAGGAAGGAAATA
AATCATTCCGAGATGTGGACGCTTCTGAGATCAGATTGATTCAGAAAACG
AAAAGTATAGAAAAATCCGAAAGTAAGAGCACTGACAAAGCCTCAAAATT
CGACAGACAAAACAGCGCCACCAGCAGAACATCAAACGATTCCAGAGAT
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protein sequence of SMED30009033-orf-1

>SMED30009033-orf-1 ID=SMED30009033-orf-1|Name=SMED30009033-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=316bp
LYKSYMMANADEFKLLLQNGDIVDDCDNKPHVAKLGQVEAKYYLDFSMAD
RVNAYHNKLFQNISDLQLDEIYKLKSNKVKSYIKIIEKYEYKGELFIKYL
TEDGRINFKPASFFKEIPKKARKPRTFPPADNQGARKPSIVIHEANDFVT
KPRYYIITDANCEEIGSIILIHNRATKMLNTDKMISKTYQIKQLNEIDRD
QAFYLSKFPANESINEWKCETFEQIKALEIGQECRYKNDRENIYKITAKN
FTEKGIYFSIQPKEGNKSFRDVDASEIRLIQKTKSIEKSESKSTDKASKF
DRQNSATSRTSNDSRD
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InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..310
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..310