SMED30009017
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009017 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30009017 aligns in the following genomic locations:
Homology
BLAST of SMED30009017 vs. Planmine SMEST
Match: SMESG000020517.1 (SMESG000020517.1) HSP 1 Score: 980.319 bits (2533), Expect = 0.000e+0 Identity = 510/512 (99.61%), Postives = 510/512 (99.61%), Query Frame = 1 Query: 118 MKIFKLDIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGXXXXXXXXXXXXXXXXNNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPNLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIFFNNN 1653 MKIFKLDIFEKVLKFYVL YFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYP LSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIFFNNN Sbjct: 1 MKIFKLDIFEKVLKFYVLLYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPKLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIFFNNN 512
BLAST of SMED30009017 vs. Planmine SMEST
Match: SMESG000040891.1 (SMESG000040891.1) HSP 1 Score: 317.39 bits (812), Expect = 1.396e-101 Identity = 201/525 (38.29%), Postives = 306/525 (58.29%), Query Frame = 1 Query: 127 FKLDIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEK-LESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILI-------QFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFY---NSAHYEIQETEKLLIDEINLRTGASE-EDIIASSNPTISFSFKVFQSINLPPFSLGXXXXXXXXXXXXXXXXNNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPNLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLF----DIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIFFNNN 1653 +K +IFEK+L+ LFYFV GF Y W CKELD S ++I F+NPIA+ + +++ + + +I S K++ E+ +E N+L Y + P FLATIV++ +++ + +M+ E ND + +GE+ LFI LI FL SK ++ FV+L + GI+N +HM+ QQI NFI IAS + SK+YN S ++P + +I ++QP VILFC + YLI FY S+ + + L +E + SE E++I N + + K S LPP SL L IL ITV+ +AI N+++ S GQ +S+IFPIPI + S+FLL +Q+ ++ RW K+ ++ ++D ++W+GA GQ+ +Q+ + ++ P L Y +L QT+ QTL ISL+LDR+W P+ +SW EY+CP VG+LS+N+SF+I ++F D++N + E LF+++F NGF S G L ILQRLHSSLIF K+I + NN Sbjct: 5 YKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITI--SQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTILEITVFSMKLHE-NDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKM------KLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNS-TKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAAKDCYYFVLILQTILQTLTISLSLDREWISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQMAKNNITE----LFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTNN 515
BLAST of SMED30009017 vs. Planmine SMEST
Match: SMESG000040922.1 (SMESG000040922.1) HSP 1 Score: 307.375 bits (786), Expect = 6.990e-98 Identity = 186/511 (36.40%), Postives = 302/511 (59.10%), Query Frame = 1 Query: 136 DIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVN-----YFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFS-----FKVFQSINLPPFSLGXXXXXXXXXXXXXXXXNNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPNLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMI 1638 D+FE++L F+ L YF++G+L NYLW C LD S +K I +F+NPIA+ T + + + + ++I +++ E L+YT+++P+FL T+ + ++++IL+ + NDF + E+ LF I LI+ K +VN Y Q++ ++F+ + G N + + MS QQI+NF+K+AS F +FY S++ T + +L INS+L P ILFC L + LF+N + + Q T L ++ +I T + F +S+N+ P L LA++F+ ++ F+ I N+ +NS ++ GQLIS++FPIP Y +I LL IQ+N+I W+K+ T+++ +D+M+LW+GA GQ+V A++ M + N S IIA+Y +L Q QT+ +++AL+RKW +PKL+SW E L PP+ +LSLN+ F+ S+ + N + + ++ LF+RIF NGFQ G+ LAI R+HSS+IFGKMI Sbjct: 8 DLFEQILNFFALSYFILGYLTNYLWACNRLDYTSSYKEIFKFWNPIAVITGISNIIIGILIIIALNVRINRVSIP-NHFEYQTLIYTLLVPSFLGTVTFMAIRIILTIKSYMNFNDFTMVLSFSEMILFCCGIGLIR---KKISMVNPEVNCYLQKL-VEFINKSALIGFCNCLIHFMSAQQIDNFVKVASNFSFYFQFYGKSLTNRTVDYYNLSNINSYLVPIAILFC--CLCVICLFFNLLNNKSQNT---------LTEDQNDSQVIVQWTSTTNLLENEPIFDRVRSLNMSPLLLILALIFLAISIFLTIIINSFLNSYQYNITGQLISTVFPIPFYFLAIILLAIQINEIMEWKKISTNEFTVDKMMLWIGAAGQLVICFAKLFMEFIERTENFSALIIAKYGLLLVQIFIQTVALNMALNRKWLRPKLKSWQEKLFPPISLLSLNMGFLFISMLNDFNRNTSHSTVKNIDKRLFLRIFGNGFQLPGLILAIFHRMHSSVIFGKMI 502
BLAST of SMED30009017 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1) HSP 1 Score: 260.381 bits (664), Expect = 7.440e-80 Identity = 190/512 (37.11%), Postives = 289/512 (56.45%), Query Frame = 1 Query: 127 FKLDIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRI-YIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSI-SQPTDNESSLIKINSFLQPFVILFCFVILSYLILFY---NSAHYEIQETEKL--LIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGXXXXXXXXXXXXXXXXNNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPNLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINT---KSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGK 1632 +K DI EK+L+F L YFV+G L +Y W KELD K+ RIL F+ PIA +++ ++ + + KK E NIL Y + IP L ++ Y + +I F +F GE+ FI+ I+ I F SK I+ Q + F+ L+ I G+VN +H+ QQI NFI +AS K N +I + P D +S + +++QP VILFC + + YL++ + N+ + I E ++ LI+ ++ + S+ S N SF +++ LPP SL L IL IT + II I N+ +N GQ+IS+ F IYL SI LLI+Q+ ++ RW K + ++D+++ W+G GQ++ AQIS+N Y++ P S +++ EY L Q + Q + ++LALDR+ PK+++W E++CP VG+LS+NL + SLFD+IN KS N + + +F++IF GFQSFG L+ILQRLHS LIFGK Sbjct: 5 YKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYKNSKKKIMKKSHKEINILFYILSIPLLLVSMSYLILLIIRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDS----LRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQ----TSKNSNKSF----MKNLQLPPISLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPA-SAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVINANSQKSFNSYETD----MFIQIFGAGFQSFGETLSILQRLHSLLIFGK 499
BLAST of SMED30009017 vs. Planmine SMEST
Match: SMESG000040928.1 (SMESG000040928.1) HSP 1 Score: 190.66 bits (483), Expect = 1.111e-53 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 1 Query: 118 MKIFKLDIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDF--VSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQ-RIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGAS----EEDIIASSNPTIS--FSFKVFQSINLPPFSLGXXXXXXXXXXXXXXXXNNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYY-PNLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMI 1638 +K + D+ LK +LF F +G NY W C LD + L F+ PIA +MD V L+ I W+ + D S IL + IP F+ + ++ V I+ ++ S F F + E FI + L + + ++ N + + Y F++L++ G+ +++ QI +FIK+A + S FYN + + +S + +LQP I+FCFV SYLI S +T+ DE + G S + I S+N IS + FK+ P FS+ L ILF T+T ++ IA+ + +S G+ I+ + IY+ SI +L++QM ++ RW K + ++D +L++G GQ+ ++ + Y Y S I + IL Q Q LVI L+L+RKW P +++W E++CP + NL + FS+F+I+N + E P LF++IF NGF S + LAILQR HSS+IF K+I Sbjct: 2 VKFYLFDVIRNWLKLLILFSFTVGIFTNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFY---SKILFLFLSIPLFIGSQIHL-VWHIVGDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQ---FDECSRLRGDSLSHENRESIESTNSNISNYYYFKI------PSFSIILGILFFTMTLILVLIASTLKSSE----SGKTITVSYAFSIYIVSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGTAGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQ----ESFP-LFLKIFGNGFYSISMTLAILQRFHSSIIFYKLI 496 The following BLAST results are available for this feature:
BLAST of SMED30009017 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009017 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009017 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009017 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009017 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009017 ID=SMED30009017|Name=SMED30009017|organism=Schmidtea mediterranea sexual|type=transcript|length=1720bpback to top protein sequence of SMED30009017-orf-1 >SMED30009017-orf-1 ID=SMED30009017-orf-1|Name=SMED30009017-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=515bp MKMKIFKLDIFEKVLKFYVLFYFVMGFLVNYLWICKELDMKSDFKRILEFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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