Voltage-dependent N-type calcium channel subunit alpha

Overview


Name	Voltage-dependent N-type calcium channel subunit alpha
Smed ID	SMED30008999
Length (bp)	794

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Voltage-dependent N-type calcium channel subunit alpha (SMED30008999) t-SNE clustered cells

Violin plots show distribution of expression levels for Voltage-dependent N-type calcium channel subunit alpha (SMED30008999) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30008999

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
gut	SMED30008999	h1SMcG0020675	Contig6657	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30008999	h1SMcG0020675	Contig6657	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30008999	h1SMcG0020675	Contig6657	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30008999	h1SMcG0020675	Contig6657	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
prepharyngeal region	SMED30008999	h1SMcG0020675	SmedASXL_014139	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30008999	h1SMcG0020675	SmedASXL_014139	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
epidermis	SMED30008999	h1SMcG0020675	dd_Smed_v4_15815_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30008999	h1SMcG0020675	dd_Smed_v4_15815_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30008999	h1SMcG0020675	dd_Smed_v6_15815_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 162.54 bits (410), Expect = 1.071e-51
Identity = 78/149 (52.35%), Postives = 105/149 (70.47%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 1.071e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.096e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1185 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1311

HSP 4 Score: 34.2686 bits (77), Expect = 8.096e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1125 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1171

HSP 5 Score: 63.1586 bits (152), Expect = 1.284e-10
Identity = 38/122 (31.15%), Postives = 67/122 (54.92%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1505 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1622

HSP 6 Score: 52.7582 bits (125), Expect = 3.325e-7
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 1.071e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.096e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1184 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1310

HSP 4 Score: 34.2686 bits (77), Expect = 8.096e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1124 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1170

HSP 5 Score: 63.1586 bits (152), Expect = 1.284e-10
Identity = 38/122 (31.15%), Postives = 67/122 (54.92%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1504 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1621

HSP 6 Score: 52.7582 bits (125), Expect = 3.325e-7
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 1.071e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.096e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1185 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1311

HSP 4 Score: 34.2686 bits (77), Expect = 8.096e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1125 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1171

HSP 5 Score: 62.7734 bits (151), Expect = 1.806e-10
Identity = 38/120 (31.67%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1505 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1620

HSP 6 Score: 52.7582 bits (125), Expect = 3.356e-7
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 1.071e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.096e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1184 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1310

HSP 4 Score: 34.2686 bits (77), Expect = 8.096e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1124 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1170

HSP 5 Score: 62.7734 bits (151), Expect = 1.805e-10
Identity = 38/120 (31.67%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1504 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1619

HSP 6 Score: 52.7582 bits (125), Expect = 3.355e-7
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 161.77 bits (408), Expect = 1.690e-51
Identity = 78/149 (52.35%), Postives = 105/149 (70.47%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 1.690e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.1149 bits (209), Expect = 9.866e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1184 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1310

HSP 4 Score: 34.2686 bits (77), Expect = 9.866e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1124 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1170

HSP 5 Score: 61.6178 bits (148), Expect = 4.477e-10
Identity = 41/123 (33.33%), Postives = 68/123 (55.28%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF-------NNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF V V+G I           NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1504 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDF-VTVLGSITDILVTEIAVKNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1621

HSP 6 Score: 52.7582 bits (125), Expect = 3.597e-7
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 127.102 bits (318), Expect = 3.553e-41
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------------GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            E YF+ IF +E V+K+IA GF L++ +YLR+ WN++DF+V+V G++                ++ D R + A+R LRPLKL+  I SLQ+V+K++  AM PLLQIG+L++F I++F+IIGLEFYSG FH  C+N+
Sbjct:  291 EPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNE 425

HSP 2 Score: 59.6918 bits (143), Expect = 3.553e-41
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDK 323
            + K TL  + P     PS SLFIF+EDN+IR+ A+ +I     E F+ L I+ NC+VL++E H P+ DK
Sbjct:  213 VRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDK 280

HSP 3 Score: 69.3218 bits (168), Expect = 3.875e-13
Identity = 40/135 (29.63%), Postives = 71/135 (52.59%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVI--------GIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +F  E ++K+I  G +L+  +Y R+ WN+LD +V+          G  GS   N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+      +F++I ++ ++GKF F C +K
Sbjct: 1032 QYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFF-CTDK 1165

HSP 4 Score: 55.0694 bits (131), Expect = 2.140e-8
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVI--GALRALRPLKLIYNIK---SLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            +FT+E+++KI+A G       Y R+ WN  DF+ +V  I  +    F    +  G LR  R  +LI  ++   +++I++    Q+ K L  + +LI  +  +++I+G++ +
Sbjct: 1366 VFTVESILKILAFGV----RNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVF 1472

HSP 5 Score: 50.0618 bits (118), Expect = 8.744e-7
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+AE  FL IF +E ++K+ A+G      TY  + +N  D +VIV      I    +  +F   V+ ALR LR  KL     SL+ ++++L  +M+ ++ +  L+   IL+F+++G++ + G+F+F
Sbjct:  700 KYAEFVFLGIFVVEMLLKLFAMG----SRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNF 821

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 126.716 bits (317), Expect = 4.333e-41
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------------GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            E YF+ IF +E V+K+IA GF L++ +YLR+ WN++DF+V+V G++                ++ D R + A+R LRPLKL+  I SLQ+V+K++  AM PLLQIG+L++F I++F+IIGLEFYSG FH  C+N+
Sbjct:  247 EPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNE 381

HSP 2 Score: 59.6918 bits (143), Expect = 4.333e-41
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDK 323
            + K TL  + P     PS SLFIF+EDN+IR+ A+ +I     E F+ L I+ NC+VL++E H P+ DK
Sbjct:  169 VRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDK 236

HSP 3 Score: 69.3218 bits (168), Expect = 4.405e-13
Identity = 40/135 (29.63%), Postives = 71/135 (52.59%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVI--------GIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +F  E ++K+I  G +L+  +Y R+ WN+LD +V+          G  GS   N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+      +F++I ++ ++GKF F C +K
Sbjct:  988 QYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFF-CTDK 1121

HSP 4 Score: 55.0694 bits (131), Expect = 2.427e-8
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVI--GALRALRPLKLIYNIK---SLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            +FT+E+++KI+A G       Y R+ WN  DF+ +V  I  +    F    +  G LR  R  +LI  ++   +++I++    Q+ K L  + +LI  +  +++I+G++ +
Sbjct: 1322 VFTVESILKILAFGV----RNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVF 1428

HSP 5 Score: 50.0618 bits (118), Expect = 9.134e-7
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+AE  FL IF +E ++K+ A+G      TY  + +N  D +VIV      I    +  +F   V+ ALR LR  KL     SL+ ++++L  +M+ ++ +  L+   IL+F+++G++ + G+F+F
Sbjct:  656 KYAEFVFLGIFVVEMLLKLFAMG----SRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNF 777

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 126.716 bits (317), Expect = 4.987e-41
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------------GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            E YF+ IF +E V+K+IA GF L++ +YLR+ WN++DF+V+V G++                ++ D R + A+R LRPLKL+  I SLQ+V+K++  AM PLLQIG+L++F I++F+IIGLEFYSG FH  C+N+
Sbjct:  239 EPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNE 373

HSP 2 Score: 59.6918 bits (143), Expect = 4.987e-41
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDK 323
            + K TL  + P     PS SLFIF+EDN+IR+ A+ +I     E F+ L I+ NC+VL++E H P+ DK
Sbjct:  161 VRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDK 228

HSP 3 Score: 68.9366 bits (167), Expect = 4.969e-13
Identity = 40/135 (29.63%), Postives = 71/135 (52.59%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVI--------GIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +F  E ++K+I  G +L+  +Y R+ WN+LD +V+          G  GS   N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+      +F++I ++ ++GKF F C +K
Sbjct:  980 QYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFF-CTDK 1113

HSP 4 Score: 55.0694 bits (131), Expect = 2.539e-8
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVI--GALRALRPLKLIYNIK---SLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            +FT+E+++KI+A G       Y R+ WN  DF+ +V  I  +    F    +  G LR  R  +LI  ++   +++I++    Q+ K L  + +LI  +  +++I+G++ +
Sbjct: 1314 VFTVESILKILAFGV----RNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVF 1420

HSP 5 Score: 50.0618 bits (118), Expect = 9.131e-7
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+AE  FL IF +E ++K+ A+G      TY  + +N  D +VIV      I    +  +F   V+ ALR LR  KL     SL+ ++++L  +M+ ++ +  L+   IL+F+++G++ + G+F+F
Sbjct:  648 KYAEFVFLGIFVVEMLLKLFAMG----SRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNF 769

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 126.331 bits (316), Expect = 5.933e-41
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------------GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            E YF+ IF +E V+K+IA GF L++ +YLR+ WN++DF+V+V G++                ++ D R + A+R LRPLKL+  I SLQ+V+K++  AM PLLQIG+L++F I++F+IIGLEFYSG FH  C+N+
Sbjct:  207 EPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNE 341

HSP 2 Score: 59.6918 bits (143), Expect = 5.933e-41
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDK 323
            + K TL  + P     PS SLFIF+EDN+IR+ A+ +I     E F+ L I+ NC+VL++E H P+ DK
Sbjct:  129 VRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDK 196

HSP 3 Score: 68.9366 bits (167), Expect = 5.496e-13
Identity = 40/135 (29.63%), Postives = 71/135 (52.59%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVI--------GIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +F  E ++K+I  G +L+  +Y R+ WN+LD +V+          G  GS   N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+      +F++I ++ ++GKF F C +K
Sbjct:  948 QYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFF-CTDK 1081

HSP 4 Score: 54.6842 bits (130), Expect = 2.829e-8
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVI--GALRALRPLKLIYNIK---SLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            +FT+E+++KI+A G       Y R+ WN  DF+ +V  I  +    F    +  G LR  R  +LI  ++   +++I++    Q+ K L  + +LI  +  +++I+G++ +
Sbjct: 1282 VFTVESILKILAFGV----RNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVF 1388

HSP 5 Score: 50.0618 bits (118), Expect = 9.716e-7
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+AE  FL IF +E ++K+ A+G      TY  + +N  D +VIV      I    +  +F   V+ ALR LR  KL     SL+ ++++L  +M+ ++ +  L+   IL+F+++G++ + G+F+F
Sbjct:  616 KYAEFVFLGIFVVEMLLKLFAMG----SRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNF 737

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 125.561 bits (314), Expect = 9.505e-41
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------------GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            E YF+ IF +E V+K+IA GF L++ +YLR+ WN++DF+V+V G++                ++ D R + A+R LRPLKL+  I SLQ+V+K++  AM PLLQIG+L++F I++F+IIGLEFYSG FH  C+N+
Sbjct:  177 EPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNE 311

HSP 2 Score: 59.6918 bits (143), Expect = 9.505e-41
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDK 323
            + K TL  + P     PS SLFIF+EDN+IR+ A+ +I     E F+ L I+ NC+VL++E H P+ DK
Sbjct:   99 VRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDK 166

HSP 3 Score: 68.5514 bits (166), Expect = 7.742e-13
Identity = 40/135 (29.63%), Postives = 71/135 (52.59%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVI--------GIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +F  E ++K+I  G +L+  +Y R+ WN+LD +V+          G  GS   N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+      +F++I ++ ++GKF F C +K
Sbjct:  910 QYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFF-CTDK 1043

HSP 4 Score: 54.299 bits (129), Expect = 3.923e-8
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVI--GALRALRPLKLIYNIK---SLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            +FT+E+++KI+A G       Y R+ WN  DF+ +V  I  +    F    +  G LR  R  +LI  ++   +++I++    Q+ K L  + +LI  +  +++I+G++ +
Sbjct: 1244 VFTVESILKILAFGV----RNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVF 1350

HSP 5 Score: 49.6766 bits (117), Expect = 1.284e-6
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+AE  FL IF +E ++K+ A+G      TY  + +N  D +VIV      I    +  +F   V+ ALR LR  KL     SL+ ++++L  +M+ ++ +  L+   IL+F+++G++ + G+F+F
Sbjct:  578 KYAEFVFLGIFVVEMLLKLFAMG----SRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNF 699

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0474241)

HSP 1 Score: 142.51 bits (358), Expect = 7.197e-45
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFN---NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +++K +  E YFL IF +EA +KI+ALG VL++++YLRN WN++DF V+V G +          D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L++F I++F+IIGLEFYSG  H  C++ E  N    + E   + PC
Sbjct:   69 LAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNK--LVKEGESETPC 218

HSP 2 Score: 56.9954 bits (136), Expect = 7.197e-45
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  174 SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            SLFI +EDN IRK  +F+I     E  V L I+ NC+VLALE H P GDK++
Sbjct:   17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTV 68

HSP 3 Score: 76.2554 bits (186), Expect = 2.296e-19
Identity = 39/124 (31.45%), Postives = 70/124 (56.45%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            F  +FTIE ++KI+ LG +L+  +YLR  WN++D +V++   +   F         N    + +  LR LRPLK I  +  L+ V   +  ++K ++ I I+ +    +FS+IG++ ++GKF +
Sbjct:  809 FTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFY 932

HSP 4 Score: 37.3502 bits (85), Expect = 2.296e-19
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            P  S+FI S  N IR+ A +V+NL   + F+ ++I  + I LA E
Sbjct:  745 PYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAE 789

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307323)

HSP 1 Score: 141.739 bits (356), Expect = 1.135e-44
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFN---NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +++K +  E YFL IF +EA +KI+ALG VL++++YLRN WN++DF V+V G +          D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L++F I++F+IIGLEFYSG  H  C++ E  N    + E   + PC
Sbjct:   69 LAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNK--LVKEGESETPC 218

HSP 2 Score: 56.9954 bits (136), Expect = 1.135e-44
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  174 SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            SLFI +EDN IRK  +F+I     E  V L I+ NC+VLALE H P GDK++
Sbjct:   17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTV 68

HSP 3 Score: 75.8702 bits (185), Expect = 3.039e-19
Identity = 39/124 (31.45%), Postives = 70/124 (56.45%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            F  +FTIE ++KI+ LG +L+  +YLR  WN++D +V++   +   F         N    + +  LR LRPLK I  +  L+ V   +  ++K ++ I I+ +    +FS+IG++ ++GKF +
Sbjct:  809 FTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFY 932

HSP 4 Score: 37.3502 bits (85), Expect = 3.039e-19
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            P  S+FI S  N IR+ A +V+NL   + F+ ++I  + I LA E
Sbjct:  745 PYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAE 789

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307325)

HSP 1 Score: 141.739 bits (356), Expect = 1.233e-44
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFN---NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +++K +  E YFL IF +EA +KI+ALG VL++++YLRN WN++DF V+V G +          D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L++F I++F+IIGLEFYSG  H  C++ E  N    + E   + PC
Sbjct:  175 LAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNK--LVKEGESETPC 324

HSP 2 Score: 56.9954 bits (136), Expect = 1.233e-44
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  174 SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            SLFI +EDN IRK  +F+I     E  V L I+ NC+VLALE H P GDK++
Sbjct:  123 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTV 174

HSP 3 Score: 75.8702 bits (185), Expect = 3.140e-19
Identity = 39/124 (31.45%), Postives = 70/124 (56.45%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            F  +FTIE ++KI+ LG +L+  +YLR  WN++D +V++   +   F         N    + +  LR LRPLK I  +  L+ V   +  ++K ++ I I+ +    +FS+IG++ ++GKF +
Sbjct:  915 FTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFY 1038

HSP 4 Score: 37.7354 bits (86), Expect = 3.140e-19
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            P  S+FI S  N IR+ A +V+NL   + F+ ++I  + I LA E
Sbjct:  851 PYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAE 895

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0332391)

HSP 1 Score: 141.354 bits (355), Expect = 1.634e-44
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFN---NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +++K +  E YFL IF +EA +KI+ALG VL++++YLRN WN++DF V+V G +          D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L++F I++F+IIGLEFYSG  H  C++ E  N    + E   + PC
Sbjct:   69 LAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNK--LVKEGESETPC 218

HSP 2 Score: 56.9954 bits (136), Expect = 1.634e-44
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  174 SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            SLFI +EDN IRK  +F+I     E  V L I+ NC+VLALE H P GDK++
Sbjct:   17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTV 68

HSP 3 Score: 75.8702 bits (185), Expect = 3.642e-19
Identity = 39/124 (31.45%), Postives = 70/124 (56.45%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            F  +FTIE ++KI+ LG +L+  +YLR  WN++D +V++   +   F         N    + +  LR LRPLK I  +  L+ V   +  ++K ++ I I+ +    +FS+IG++ ++GKF +
Sbjct:  809 FTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFY 932

HSP 4 Score: 37.3502 bits (85), Expect = 3.642e-19
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            P  S+FI S  N IR+ A +V+NL   + F+ ++I  + I LA E
Sbjct:  745 PYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAE 789

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307320)

HSP 1 Score: 140.584 bits (353), Expect = 3.167e-44
Identity = 72/159 (45.28%), Postives = 107/159 (67.30%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG---IIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +++K +  E YFL IF +EA +KI+ALG VL++++YLRN WN++DF V+V G   I      + D R++ + R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L++F I++F+IIGLEFYSG  H  C++ E  N    + E   + PC
Sbjct:   62 PGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGAMTIFAEANIDVDLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNK--LVKEGESETPC 218

HSP 2 Score: 56.9954 bits (136), Expect = 3.167e-44
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  174 SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            SLFI +EDN IRK  +F+I     E  V L I+ NC+VLALE H P GDK++
Sbjct:   17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTV 68

HSP 3 Score: 72.4034 bits (176), Expect = 4.053e-18
Identity = 40/137 (29.20%), Postives = 75/137 (54.74%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ---------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQ 739
            F  +FTIE ++KI+ LG +L+  +YLR  WN++D +V++   +   F+             + +  LR LRPLK I  +  L+ V   +  ++K ++ I I+ +    +FS+IG++ ++GKF +     + ++ E Q
Sbjct:  809 FTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQ 945

HSP 4 Score: 37.3502 bits (85), Expect = 4.053e-18
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            P  S+FI S  N IR+ A +V+NL   + F+ ++I  + I LA E
Sbjct:  745 PYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAE 789

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1aa (calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a [Source:ZFIN;Acc:ZDB-GENE-040724-26])

HSP 1 Score: 152.525 bits (384), Expect = 5.039e-49
Identity = 72/138 (52.17%), Postives = 103/138 (74.64%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            P   +  +S++ +  E YF+ IF  E+ IKI+ALGF  ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF+K
Sbjct:  115 PDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDK 252

HSP 2 Score: 61.2326 bits (147), Expect = 5.039e-49
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN +RK A+ +   +  E  +   I+ NCIVLALE H P+GDK+
Sbjct:   68 NRSLFIFSEDNFVRKYAKKITECTPFEYMILATIIANCIVLALEQHLPDGDKT 120

HSP 3 Score: 78.9518 bits (193), Expect = 3.583e-16
Identity = 40/133 (30.08%), Postives = 76/133 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +FT E +IK++ LG VL+Q +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + G+F F C ++
Sbjct: 1158 RYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRF-FYCTDE 1289

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1aa (calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a [Source:ZFIN;Acc:ZDB-GENE-040724-26])

HSP 1 Score: 152.525 bits (384), Expect = 5.166e-49
Identity = 72/138 (52.17%), Postives = 103/138 (74.64%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            P   +  +S++ +  E YF+ IF  E+ IKI+ALGF  ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF+K
Sbjct:  115 PDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDK 252

HSP 2 Score: 61.2326 bits (147), Expect = 5.166e-49
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN +RK A+ +   +  E  +   I+ NCIVLALE H P+GDK+
Sbjct:   68 NRSLFIFSEDNFVRKYAKKITECTPFEYMILATIIANCIVLALEQHLPDGDKT 120

HSP 3 Score: 78.9518 bits (193), Expect = 3.549e-16
Identity = 40/133 (30.08%), Postives = 76/133 (57.14%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            ++ +  F  +FT E +IK++ LG VL+Q +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + G+F F C ++
Sbjct: 1158 RYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRF-FYCTDE 1289

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ab (calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-3])

HSP 1 Score: 154.451 bits (389), Expect = 9.362e-49
Identity = 71/137 (51.82%), Postives = 99/137 (72.26%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFN 712
            P   +  +S++ +  E YF+ IF  E+ IKI+ALGF  ++ +YLRN WNV+DF+V++ GI+ S  +  D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+   ILMF+IIGLEFY GKFH  CFN
Sbjct:  116 PVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFN 252

HSP 2 Score: 58.5362 bits (140), Expect = 9.362e-49
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   69 NRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKT 121

HSP 3 Score: 81.6481 bits (200), Expect = 5.735e-17
Identity = 36/128 (28.12%), Postives = 72/128 (56.25%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG +L++ +Y R+ WN LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + G+F +
Sbjct: 1133 RYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFY 1260

HSP 4 Score: 60.4622 bits (145), Expect = 7.293e-10
Identity = 35/117 (29.91%), Postives = 65/117 (55.56%), Query Frame = 2

Query:  350 LYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG-----IIGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            + F T F +E+++KIIA G       + R+ WN+ DF V VIG     ++    NNF +   +   RA R +KL+   ++++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1459 IVFTTFFFMESILKIIAFGV----RNFFRDAWNIFDF-VSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLF 1570

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ab (calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-3])

HSP 1 Score: 154.451 bits (389), Expect = 1.017e-48
Identity = 71/137 (51.82%), Postives = 99/137 (72.26%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFN 712
            P   +  +S++ +  E YF+ IF  E+ IKI+ALGF  ++ +YLRN WNV+DF+V++ GI+ S  +  D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+   ILMF+IIGLEFY GKFH  CFN
Sbjct:  116 PVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFN 252

HSP 2 Score: 58.151 bits (139), Expect = 1.017e-48
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   69 NRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKT 121

HSP 3 Score: 81.6481 bits (200), Expect = 5.727e-17
Identity = 36/128 (28.12%), Postives = 72/128 (56.25%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG +L++ +Y R+ WN LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + G+F +
Sbjct: 1133 RYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFY 1260

HSP 4 Score: 60.4622 bits (145), Expect = 7.284e-10
Identity = 35/117 (29.91%), Postives = 65/117 (55.56%), Query Frame = 2

Query:  350 LYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG-----IIGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            + F T F +E+++KIIA G       + R+ WN+ DF V VIG     ++    NNF +   +   RA R +KL+   ++++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1459 IVFTTFFFMESILKIIAFGV----RNFFRDAWNIFDF-VSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLF 1570

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 162.155 bits (409), Expect = 1.198e-47
Identity = 77/156 (49.36%), Postives = 108/156 (69.23%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++++YLRN WN++DF+V+V GI+ +  + FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY GKFHF CF  +         E A+  PC
Sbjct:  101 PALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTG-------EKAVDYPC 249

HSP 2 Score: 46.9802 bits (110), Expect = 1.198e-47
Identity = 26/53 (49.06%), Postives = 31/53 (58.49%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK              +   I+ NCIVLALE H P  DK+
Sbjct:   65 NRSLFIFSEDNVIRKY-----------YLILTTIIANCIVLALEQHLPALDKT 106

HSP 3 Score: 81.6481 bits (200), Expect = 6.246e-23
Identity = 44/131 (33.59%), Postives = 72/131 (54.96%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVI--------VIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+          G  G   N    + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF F C
Sbjct: 1126 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTI--KSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKF-FHC 1253

HSP 4 Score: 44.669 bits (104), Expect = 6.246e-23
Identity = 26/87 (29.89%), Postives = 45/87 (51.72%), Query Frame = 3

Query:   72 GNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE----VHQPEGD 320
            G+S + N+  + + +L++N      P  HI P  S+F+F   N +R++  +V+NL   E+ +  +I  + I LA E     H P  D
Sbjct: 1043 GDSLLLNEEKKDLDELNQNA-----PK-HILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRND 1123

HSP 5 Score: 58.9214 bits (141), Expect = 2.075e-9
Identity = 33/120 (27.50%), Postives = 65/120 (54.17%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-DQRV----IGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E V+K+IA G +     YL+  WN+ DF+ ++  I         D+R+    +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1447 KWLNIIFTALFTLECVLKVIAFGPL----NYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1562

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: ENSXETT00000009772.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460576.1:33700:87270:-1 gene:ENSXETG00000001762.1 transcript:ENSXETT00000009772.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 152.525 bits (384), Expect = 1.313e-45
Identity = 73/143 (51.05%), Postives = 103/143 (72.03%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSND 730
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIGLEFY GKFH  CF+ +   +
Sbjct:   69 PEHDKTDMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFSDDSGEN 211

HSP 2 Score: 50.447 bits (119), Expect = 1.313e-45
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFS+DN+IRK A+ +        +    I+ NCIVLALE H PE DK+
Sbjct:   26 NRSLFIFSDDNVIRKYAKRITEWP----YPLSTIIANCIVLALEQHLPEHDKT 74

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 148.673 bits (374), Expect = 9.598e-43
Identity = 76/153 (49.67%), Postives = 106/153 (69.28%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII---GSTFN-NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +S++ +  E YF+ IF  EA IKI+ALG V ++ +YLRN WNV+DF+V++ GI+   G+ FN + D R + A+R LRPLKL+  I SLQIV+K++ +AM PLLQIG+L+ F ILMF+IIGLEFYSGK H  C+      +   ++ P    PC
Sbjct:  143 MSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPP---HPC 292

HSP 2 Score: 44.669 bits (104), Expect = 9.598e-43
Identity = 29/76 (38.16%), Postives = 38/76 (50.00%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQ----FVINLSVV-------------------EIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIF EDN+IRK A+      +N++ V                   E  +   I+ NCIVLALE H P  DK+
Sbjct:   66 NRSLFIFGEDNIIRKYAKNNNDLYLNVNSVMFFLYPFGSVFLNSLLTPFEYMILATIIANCIVLALEQHLPGDDKT 141

HSP 3 Score: 78.5666 bits (192), Expect = 7.091e-16
Identity = 40/127 (31.50%), Postives = 73/127 (57.48%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIG---STFNNFDQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK+I  G +L++ +Y R+ WN+LDF+V+V  +I    +T    D + I +   LR LRPLK I  +  L+ V   +  ++K +  I I+    + +F++I ++ + GKF +
Sbjct:  977 RYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFY 1103

HSP 4 Score: 63.1586 bits (152), Expect = 1.268e-10
Identity = 35/124 (28.23%), Postives = 67/124 (54.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +F++E ++KIIA GF+     Y R+ WN+ DF + VIG I             ++F+   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1297 KYLNIAFTMLFSLECILKIIAFGFL----NYFRDTWNIFDF-ITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1415

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 148.673 bits (374), Expect = 1.009e-42
Identity = 76/153 (49.67%), Postives = 106/153 (69.28%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII---GSTFN-NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +S++ +  E YF+ IF  EA IKI+ALG V ++ +YLRN WNV+DF+V++ GI+   G+ FN + D R + A+R LRPLKL+  I SLQIV+K++ +AM PLLQIG+L+ F ILMF+IIGLEFYSGK H  C+      +   ++ P    PC
Sbjct:  143 MSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPP---HPC 292

HSP 2 Score: 44.669 bits (104), Expect = 1.009e-42
Identity = 29/75 (38.67%), Postives = 37/75 (49.33%), Query Frame = 3

Query:  171 RSLFIFSEDNLIRKIAQ----FVINLSVV-------------------EIFVQLIILTNCIVLALEVHQPEGDKS 326
            RSLFIF EDN+IRK A+      +N++ V                   E  +   I+ NCIVLALE H P  DK+
Sbjct:   67 RSLFIFGEDNIIRKYAKNNNDLYLNVNSVMFFLYPFGSVFLNSLLTPFEYMILATIIANCIVLALEQHLPGDDKT 141

HSP 3 Score: 77.7962 bits (190), Expect = 1.007e-17
Identity = 40/131 (30.53%), Postives = 72/131 (54.96%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK+I  G +L++ +Y R+ WN+LDF+V+V  +I     N        D + I +   LR LRPLK I  +  L+ V   +  ++K +  I I+    + +F++I ++ + GKF +
Sbjct:  933 RYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFY 1063

HSP 4 Score: 31.5722 bits (70), Expect = 1.007e-17
Identity = 14/36 (38.89%), Postives = 22/36 (61.11%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLI 266
            I P  S+ IFS  N IRK+  +++NL   E+ + L+
Sbjct:  873 IVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLV 908

HSP 5 Score: 63.1586 bits (152), Expect = 1.340e-10
Identity = 35/124 (28.23%), Postives = 67/124 (54.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +F++E ++KIIA GF+     Y R+ WN+ DF + VIG I             ++F+   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1241 KYLNIAFTMLFSLECILKIIAFGFL----NYFRDTWNIFDF-ITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1359

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 148.288 bits (373), Expect = 1.392e-42
Identity = 76/153 (49.67%), Postives = 106/153 (69.28%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII---GSTFN-NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            +S++ +  E YF+ IF  EA IKI+ALG V ++ +YLRN WNV+DF+V++ GI+   G+ FN + D R + A+R LRPLKL+  I SLQIV+K++ +AM PLLQIG+L+ F ILMF+IIGLEFYSGK H  C+      +   ++ P    PC
Sbjct:  143 MSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPP---HPC 292

HSP 2 Score: 44.669 bits (104), Expect = 1.392e-42
Identity = 29/76 (38.16%), Postives = 38/76 (50.00%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQ----FVINLSVV-------------------EIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIF EDN+IRK A+      +N++ V                   E  +   I+ NCIVLALE H P  DK+
Sbjct:   66 NRSLFIFGEDNIIRKYAKNNNDLYLNVNSVMFFLYPFGSVFLNSLLTPFEYMILATIIANCIVLALEQHLPGDDKT 141

HSP 3 Score: 78.5666 bits (192), Expect = 8.826e-16
Identity = 40/127 (31.50%), Postives = 73/127 (57.48%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIG---STFNNFDQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK+I  G +L++ +Y R+ WN+LDF+V+V  +I    +T    D + I +   LR LRPLK I  +  L+ V   +  ++K +  I I+    + +F++I ++ + GKF +
Sbjct:  985 RYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFY 1111

HSP 4 Score: 62.7734 bits (151), Expect = 1.558e-10
Identity = 35/124 (28.23%), Postives = 67/124 (54.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +F++E ++KIIA GF+     Y R+ WN+ DF + VIG I             ++F+   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1305 KYLNIAFTMLFSLECILKIIAFGFL----NYFRDTWNIFDF-ITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1423

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 152.91 bits (385), Expect = 2.221e-41
Identity = 79/173 (45.66%), Postives = 112/173 (64.74%), Query Frame = 2

Query:  284 CFGLGSPPA-------GR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII---GSTFN-NFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            C+ +G  P         +  +S++ +  E YF+ IF  EA IKI+ALG V ++ +YLRN WNV+DF+V++ GI+   G+ FN + D R + A+R LRPLKL+  I SLQIV+K++ +AM PLLQIG+L+ F ILMF+IIGLEFYSGK H  C+      +   ++ P    PC
Sbjct:  100 CYSMGCAPLCQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPP---HPC 269

HSP 2 Score: 80.4925 bits (197), Expect = 5.091e-19
Identity = 40/127 (31.50%), Postives = 73/127 (57.48%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIG---STFNNFDQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK+I  G +L++ +Y R+ WN+LDF+V+V  +I    +T    D + I +   LR LRPLK I  +  L+ V   +  ++K +  I I+    + +F++I ++ + GKF +
Sbjct: 1048 RYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFY 1174

HSP 3 Score: 33.113 bits (74), Expect = 5.091e-19
Identity = 14/36 (38.89%), Postives = 22/36 (61.11%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLI 266
            I P  S+ IFS  N IRK+  +++NL   E+ + L+
Sbjct:  988 IVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLV 1023

HSP 4 Score: 64.6994 bits (156), Expect = 4.074e-11
Identity = 35/124 (28.23%), Postives = 67/124 (54.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +F++E ++KIIA GF+     Y R+ WN+ DF + VIG I             ++F+   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1368 KYLNIAFTMLFSLECILKIIAFGFL----NYFRDTWNIFDF-ITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1486

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 163.696 bits (413), Expect = 3.984e-52
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 3.984e-52
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.311e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1173 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1299

HSP 4 Score: 33.4982 bits (75), Expect = 8.311e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1113 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1159

HSP 5 Score: 62.7734 bits (151), Expect = 1.322e-10
Identity = 37/122 (30.33%), Postives = 67/122 (54.92%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1493 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1610

HSP 6 Score: 52.373 bits (124), Expect = 3.401e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 163.31 bits (412), Expect = 4.187e-52
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 4.187e-52
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 86.2705 bits (212), Expect = 5.205e-21
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F++F       D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1173 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1303

HSP 4 Score: 33.4982 bits (75), Expect = 5.205e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1113 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1159

HSP 5 Score: 62.003 bits (149), Expect = 1.928e-10
Identity = 37/120 (30.83%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1497 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1612

HSP 6 Score: 52.373 bits (124), Expect = 3.527e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 163.31 bits (412), Expect = 4.221e-52
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 4.221e-52
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 86.2705 bits (212), Expect = 5.248e-21
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F++F       D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1174 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1304

HSP 4 Score: 33.4982 bits (75), Expect = 5.248e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1114 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1160

HSP 5 Score: 62.003 bits (149), Expect = 1.964e-10
Identity = 37/120 (30.83%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1498 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1613

HSP 6 Score: 52.373 bits (124), Expect = 3.527e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 163.31 bits (412), Expect = 4.221e-52
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 4.221e-52
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 86.2705 bits (212), Expect = 5.248e-21
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F++F       D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1174 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1304

HSP 4 Score: 33.4982 bits (75), Expect = 5.248e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1114 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1160

HSP 5 Score: 62.003 bits (149), Expect = 1.964e-10
Identity = 37/120 (30.83%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1498 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1613

HSP 6 Score: 52.373 bits (124), Expect = 3.527e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 163.31 bits (412), Expect = 4.702e-52
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 4.702e-52
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 8.877e-21
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1172 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1298

HSP 4 Score: 33.4982 bits (75), Expect = 8.877e-21
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1112 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1158

HSP 5 Score: 62.7734 bits (151), Expect = 1.382e-10
Identity = 37/122 (30.33%), Postives = 67/122 (54.92%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1492 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1609

HSP 6 Score: 52.373 bits (124), Expect = 3.587e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|P56698|CAC1B_DIPOM (Probable voltage-dependent N-type calcium channel subunit alpha-1B OS=Diplobatis ommata OX=1870830 PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 9.702e-52
Identity = 79/156 (50.64%), Postives = 108/156 (69.23%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++    E YF+ IF  EA IKIIALGF  ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF++E        NEP  + PC
Sbjct:  112 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTTIGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKTCFSEET-------NEPVEEFPC 260

HSP 2 Score: 62.003 bits (149), Expect = 9.702e-52
Identity = 29/53 (54.72%), Postives = 37/53 (69.81%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   65 NRSLFIFSEDNIIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 117

HSP 3 Score: 85.5001 bits (210), Expect = 1.438e-21
Identity = 43/127 (33.86%), Postives = 72/127 (56.69%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF---DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+   ++   F      D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1181 KYLDYVFTGVFTFEMVIKMINLGLILHPGSYFRDLWNILDFIVVSGALVAFAFTGSRGKDLNTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1307

HSP 4 Score: 38.891 bits (89), Expect = 1.438e-21
Identity = 17/48 (35.42%), Postives = 28/48 (58.33%), Query Frame = 3

Query:  156 HINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
             I P  S+F+FS  N +R+   +++NL   E+ + L+I  + I LA E
Sbjct: 1120 QIPPFNSMFLFSTTNPVRRACHYIVNLRYFEMCILLVITMSSIALAAE 1167

HSP 5 Score: 57.3806 bits (137), Expect = 4.616e-8
Identity = 34/122 (27.87%), Postives = 66/122 (54.10%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            ++  + F  +F++E V+K+I  G VLN   Y R+ WNV DF+ ++  I       +  +F N     +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1501 QYLNILFTFLFSMECVLKLIGFG-VLN---YFRDAWNVFDFVTVLGSITDILVTELADSFINLS--FLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1616

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q05152|CAC1B_RABIT (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Oryctolagus cuniculus OX=9986 GN=CACNA1B PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.945e-51
Identity = 79/156 (50.64%), Postives = 110/156 (70.51%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++    E YF+ IF  EA IKI+ALGFVL++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF   P++ +P   +P    PC
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKILALGFVLHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACF---PNSTDP---DPVGDFPC 268

HSP 2 Score: 60.8474 bits (146), Expect = 1.945e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.8853 bits (211), Expect = 4.600e-20
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1189 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1315

HSP 4 Score: 33.4982 bits (75), Expect = 4.600e-20
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1129 IVPYSSMFCLSPTNLLRRCCHYIVTMRYFEMVILVVIALSSIALAAE 1175

HSP 5 Score: 62.7734 bits (151), Expect = 7.346e-10
Identity = 38/120 (31.67%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1509 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1624

HSP 6 Score: 52.373 bits (124), Expect = 2.446e-6
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|O55017|CAC1B_MOUSE (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Mus musculus OX=10090 GN=Cacna1b PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.413e-51
Identity = 79/158 (50.00%), Postives = 109/158 (68.99%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEPQLNEPAIKR 763
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P   +P  ++
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQ 276

HSP 2 Score: 60.8474 bits (146), Expect = 2.413e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 86.2705 bits (212), Expect = 3.002e-20
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F++F       D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1173 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1303

HSP 4 Score: 33.4982 bits (75), Expect = 3.002e-20
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1113 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1159

HSP 5 Score: 62.003 bits (149), Expect = 1.349e-9
Identity = 37/120 (30.83%), Postives = 68/120 (56.67%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1497 KCLNIVFTSMFSMECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1612

HSP 6 Score: 52.373 bits (124), Expect = 2.467e-6
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q00975|CAC1B_HUMAN (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Homo sapiens OX=9606 GN=CACNA1B PE=1 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 4.490e-51
Identity = 78/149 (52.35%), Postives = 105/149 (70.47%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 4.490e-51
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 3.395e-20
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1184 KYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1310

HSP 4 Score: 34.2686 bits (77), Expect = 3.395e-20
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1124 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1170

HSP 5 Score: 63.1586 bits (152), Expect = 6.165e-10
Identity = 38/122 (31.15%), Postives = 67/122 (54.92%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E V+KIIA G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1504 KCLNIVFTSMFSMECVLKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1621

HSP 6 Score: 52.7582 bits (125), Expect = 1.597e-6
Identity = 41/130 (31.54%), Postives = 70/130 (53.85%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F          ++F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  516 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 637

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q02294|CAC1B_RAT (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Rattus norvegicus OX=10116 GN=Cacna1b PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 4.341e-50
Identity = 77/149 (51.68%), Postives = 104/149 (69.80%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++  I+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTEILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 4.341e-50
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 86.2705 bits (212), Expect = 2.676e-20
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F++F       D   I +   LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1182 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1312

HSP 4 Score: 33.4982 bits (75), Expect = 2.676e-20
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1122 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1168

HSP 5 Score: 61.2326 bits (147), Expect = 2.715e-9
Identity = 37/120 (30.83%), Postives = 67/120 (55.83%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K   + F ++F++E ++KIIA G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL     +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1506 KCLNIVFTSMFSLECILKIIAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLCRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1621

HSP 6 Score: 52.373 bits (124), Expect = 2.316e-6
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL +F  E  +K+  LG      +Y R+ +N  DF VIV    GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  517 FAEFVFLGLFLTEMSLKMYGLG----PRSYFRSSFNCFDFGVIV----GSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 638

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A2I2V1V1 (Voltage-dependent N-type calcium channel subunit alpha OS=Felis catus OX=9685 GN=CACNA1B PE=3 SV=3)

HSP 1 Score: 164.081 bits (414), Expect = 2.389e-49
Identity = 80/156 (51.28%), Postives = 109/156 (69.87%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF   P++ +P   EP    PC
Sbjct:  118 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACF---PNSTDP---EPVGDFPC 267

HSP 2 Score: 60.8474 bits (146), Expect = 2.389e-49
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   59 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 123

HSP 3 Score: 84.7297 bits (208), Expect = 1.161e-17
Identity = 41/127 (32.28%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I ++ M  + +F++I ++ + GKF +
Sbjct: 1171 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILVVYMLFMFIFAVIAVQLFKGKFFY 1297

HSP 4 Score: 33.8834 bits (76), Expect = 1.161e-17
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1111 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1157

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A5F5XJ38 (Voltage-dependent N-type calcium channel subunit alpha OS=Felis catus OX=9685 GN=CACNA1B PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.532e-49
Identity = 80/156 (51.28%), Postives = 109/156 (69.87%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF   P++ +P   EP    PC
Sbjct:  118 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACF---PNSTDP---EPVGDFPC 267

HSP 2 Score: 60.8474 bits (146), Expect = 2.532e-49
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   59 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 123

HSP 3 Score: 84.7297 bits (208), Expect = 1.210e-17
Identity = 41/127 (32.28%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I ++ M  + +F++I ++ + GKF +
Sbjct: 1171 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILVVYMLFMFIFAVIAVQLFKGKFFY 1297

HSP 4 Score: 33.8834 bits (76), Expect = 1.210e-17
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1111 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAE 1157

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A287APC1 (Voltage-dependent N-type calcium channel subunit alpha OS=Sus scrofa OX=9823 GN=CACNA1B PE=3 SV=2)

HSP 1 Score: 163.696 bits (413), Expect = 2.842e-49
Identity = 79/149 (53.02%), Postives = 106/149 (71.14%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFVL++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVLHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 2.842e-49
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 4.901e-18
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1176 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1302

HSP 4 Score: 34.2686 bits (77), Expect = 4.901e-18
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1116 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1162

HSP 5 Score: 60.8474 bits (146), Expect = 9.758e-7
Identity = 35/116 (30.17%), Postives = 65/116 (56.03%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-------IGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F ++F++E ++KI+A G VLN   Y R+ WNV DF+ ++  I       I  T N  +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1502 FTSMFSMECMLKIVAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1613

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A286ZQ69 (Voltage-dependent N-type calcium channel subunit alpha OS=Sus scrofa OX=9823 GN=CACNA1B PE=3 SV=2)

HSP 1 Score: 163.696 bits (413), Expect = 2.842e-49
Identity = 79/149 (53.02%), Postives = 106/149 (71.14%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCF----NKEPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFVL++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF    + EP  D P
Sbjct:  119 PDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVLHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFP 267

HSP 2 Score: 60.8474 bits (146), Expect = 2.842e-49
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  126 NTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            N + +    F +N  RSLF+FSEDN++RK A+ +      E  +   I+ NCIVLALE H P+GDK+
Sbjct:   60 NPIPVKQNCFTVN--RSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKT 124

HSP 3 Score: 85.5001 bits (210), Expect = 4.901e-18
Identity = 42/127 (33.07%), Postives = 71/127 (55.91%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+   Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1176 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1302

HSP 4 Score: 34.2686 bits (77), Expect = 4.901e-18
Identity = 14/47 (29.79%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  159 INPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            I P  S+F  S  NL+R+   +++ +   E+ + ++I  + I LA E
Sbjct: 1116 IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAE 1162

HSP 5 Score: 60.4622 bits (145), Expect = 1.371e-6
Identity = 35/114 (30.70%), Postives = 66/114 (57.89%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI----IGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F ++F++E ++KI+A G VLN   Y R+ WNV DF+ ++  I    +    NNF +   +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1502 FTSMFSMECMLKIVAFG-VLN---YFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1611

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: P91744 (Voltage-dependent calcium channel OS=Heterololigo bleekeri OX=1423826 PE=2 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 3.219e-49
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF---NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK--EPSNDEPQLNEPAIKRPC 769
            E YFL IF +EA++KI+ALGF L++ +YLRN WN++DF+V+V G I S F   N+FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQ+ +L++F I++F+IIGLEFY+G FH  CF K  + S D  +  +    RPC
Sbjct:  104 EFYFLGIFCVEALLKIVALGFALHKGSYLRNVWNIMDFVVVVTGFI-SIFPASNSFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIIRAMAPLLQVCLLVLFAIVIFAIIGLEFYTGAFHKACFIKPNDDSEDNIEYGDEDTIRPC 248

HSP 2 Score: 68.1662 bits (165), Expect = 3.219e-49
Identity = 34/70 (48.57%), Postives = 44/70 (62.86%), Query Frame = 3

Query:  117 LSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            ++K    +SLP    + +RSLFIFSE+N IRK A+ +I     E  V L I+ NCIVLALE H P  DK+
Sbjct:   25 VAKKAATVSLPGMGSSTNRSLFIFSEENFIRKYAKIIIEWGPFEYMVLLTIIANCIVLALEEHLPNEDKT 94

HSP 3 Score: 79.7221 bits (195), Expect = 1.660e-18
Identity = 43/132 (32.58%), Postives = 73/132 (55.30%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF-----NNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            F  +FTIE ++KI+ LG +L+  +Y R+ WN+LD  V++  ++   F      N +  + +  LR LRPLK I  I  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   SN E
Sbjct:  935 FTGVFTIEMLLKIVDLGIILHPGSYCRDAWNILDATVVICALVAFAFGDAAGGNLNTIKSLRVLRVLRPLKTINRIPKLKAVFDCVVNSLKNVSNILIVYLLFQFIFAVIAVQLFKGKF-FYCTDMSKSNRE 1065

HSP 4 Score: 41.5874 bits (96), Expect = 1.660e-18
Identity = 20/55 (36.36%), Postives = 32/55 (58.18%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSL 329
            P  S+FIF   N +R+   FV+NL   ++F+ ++I  + I LA E   P  D+S+
Sbjct:  871 PYSSMFIFGPTNPVRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVNDESV 923

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 157.532 bits (397), Expect = 4.653e-50
Identity = 75/147 (51.02%), Postives = 107/147 (72.79%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK--EPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY GKFH  CF +  E ++D P
Sbjct:  104 PVGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHTSCFKESGELASDWP 250

HSP 2 Score: 59.6918 bits (143), Expect = 4.653e-50
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   57 NRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPVGDKT 109

HSP 3 Score: 82.4185 bits (202), Expect = 1.447e-22
Identity = 41/127 (32.28%), Postives = 74/127 (58.27%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII-----GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+   ++     GS   + +  + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF +
Sbjct: 1150 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVTGALVAFACSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFY 1276

HSP 4 Score: 42.3578 bits (98), Expect = 1.447e-22
Identity = 21/76 (27.63%), Postives = 43/76 (56.58%), Query Frame = 3

Query:   72 GNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            G+S + ++  + + +L++      +P  HI P  S+F+F + N +R++  +V+NL   E+ +  +I  + I LA E
Sbjct: 1067 GDSLLLSEEKKDLDELNQ------IPPKHILPYSSMFVFGQSNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAE 1136

HSP 5 Score: 59.3066 bits (142), Expect = 1.308e-9
Identity = 32/121 (26.45%), Postives = 62/121 (51.24%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E ++KIIA G +     YL+  WN+ DF+ ++  I         Q        +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1470 KWLNIIFTALFTLECILKIIAFGLL----NYLKEAWNIFDFVTVLGSITDILVTEIKQDKLINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1586

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 157.532 bits (397), Expect = 4.810e-50
Identity = 75/147 (51.02%), Postives = 107/147 (72.79%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK--EPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY GKFH  CF +  E ++D P
Sbjct:  104 PVGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHTSCFKESGELASDWP 250

HSP 2 Score: 59.6918 bits (143), Expect = 4.810e-50
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   57 NRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPVGDKT 109

HSP 3 Score: 82.4185 bits (202), Expect = 1.448e-22
Identity = 41/127 (32.28%), Postives = 74/127 (58.27%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII-----GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+   ++     GS   + +  + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF +
Sbjct: 1129 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVTGALVAFACSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFY 1255

HSP 4 Score: 42.3578 bits (98), Expect = 1.448e-22
Identity = 21/76 (27.63%), Postives = 43/76 (56.58%), Query Frame = 3

Query:   72 GNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            G+S + ++  + + +L++      +P  HI P  S+F+F + N +R++  +V+NL   E+ +  +I  + I LA E
Sbjct: 1046 GDSLLLSEEKKDLDELNQ------IPPKHILPYSSMFVFGQSNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAE 1115

HSP 5 Score: 59.3066 bits (142), Expect = 1.343e-9
Identity = 32/121 (26.45%), Postives = 62/121 (51.24%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E ++KIIA G +     YL+  WN+ DF+ ++  I         Q        +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1449 KWLNIIFTALFTLECILKIIAFGLL----NYLKEAWNIFDFVTVLGSITDILVTEIKQDKLINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1565

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 157.532 bits (397), Expect = 4.849e-50
Identity = 75/147 (51.02%), Postives = 107/147 (72.79%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK--EPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY GKFH  CF +  E ++D P
Sbjct:  104 PVGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHTSCFKESGELASDWP 250

HSP 2 Score: 59.6918 bits (143), Expect = 4.849e-50
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   57 NRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPVGDKT 109

HSP 3 Score: 82.4185 bits (202), Expect = 1.412e-22
Identity = 41/127 (32.28%), Postives = 74/127 (58.27%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII-----GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+   ++     GS   + +  + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF +
Sbjct: 1150 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVTGALVAFACSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFY 1276

HSP 4 Score: 42.3578 bits (98), Expect = 1.412e-22
Identity = 21/76 (27.63%), Postives = 43/76 (56.58%), Query Frame = 3

Query:   72 GNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            G+S + ++  + + +L++      +P  HI P  S+F+F + N +R++  +V+NL   E+ +  +I  + I LA E
Sbjct: 1067 GDSLLLSEEKKDLDELNQ------IPPKHILPYSSMFVFGQSNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAE 1136

HSP 5 Score: 59.3066 bits (142), Expect = 1.332e-9
Identity = 32/121 (26.45%), Postives = 62/121 (51.24%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E ++KIIA G +     YL+  WN+ DF+ ++  I         Q        +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1470 KWLNIIFTALFTLECILKIIAFGLL----NYLKEAWNIFDFVTVLGSITDILVTEIKQDKLINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1586

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 157.532 bits (397), Expect = 5.583e-50
Identity = 75/147 (51.02%), Postives = 107/147 (72.79%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK--EPSNDEP 736
            P   +  +S++    E YF+ IF  EA IKIIALGFV ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY GKFH  CF +  E ++D P
Sbjct:  104 PVGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHTSCFKESGELASDWP 250

HSP 2 Score: 59.3066 bits (142), Expect = 5.583e-50
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +      E  +   I+ NCIVLALE H P GDK+
Sbjct:   57 NRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPVGDKT 109

HSP 3 Score: 82.4185 bits (202), Expect = 1.572e-22
Identity = 41/127 (32.28%), Postives = 74/127 (58.27%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII-----GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+   ++     GS   + +  + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF +
Sbjct: 1150 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVTGALVAFACSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFY 1276

HSP 4 Score: 42.3578 bits (98), Expect = 1.572e-22
Identity = 21/76 (27.63%), Postives = 43/76 (56.58%), Query Frame = 3

Query:   72 GNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            G+S + ++  + + +L++      +P  HI P  S+F+F + N +R++  +V+NL   E+ +  +I  + I LA E
Sbjct: 1067 GDSLLLSEEKKDLDELNQ------IPPKHILPYSSMFVFGQSNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAE 1136

HSP 5 Score: 59.3066 bits (142), Expect = 1.380e-9
Identity = 32/121 (26.45%), Postives = 62/121 (51.24%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E ++KIIA G +     YL+  WN+ DF+ ++  I         Q        +   RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1470 KWLNIIFTALFTLECILKIIAFGLL----NYLKEAWNIFDFVTVLGSITDILVTEIKQDKLINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1586

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1aa (calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a [Source:ZFIN;Acc:ZDB-GENE-040724-26])

HSP 1 Score: 153.68 bits (387), Expect = 2.549e-46
Identity = 71/138 (51.45%), Postives = 101/138 (73.19%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            P   +  +S++    E YF+ IF  E+ IKI+ALGF  ++ +YLRN WN++DF+V++ GI+ S  + FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF++
Sbjct:  101 PDGDKTPMSERLDDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNMMDFVVVLTGILSSVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYIGKFHKTCFDE 238

HSP 2 Score: 50.8322 bits (120), Expect = 2.549e-46
Identity = 26/53 (49.06%), Postives = 32/53 (60.38%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN +RK    +             I+ NCIVLALE H P+GDK+
Sbjct:   65 NRSLFIFSEDNFVRKYYMILAT-----------IIANCIVLALEQHLPDGDKT 106

HSP 3 Score: 86.6557 bits (213), Expect = 8.588e-19
Identity = 40/128 (31.25%), Postives = 73/128 (57.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG VL+Q +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + GKF F
Sbjct: 1135 RYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFF 1262

HSP 4 Score: 48.521 bits (114), Expect = 4.249e-6
Identity = 41/130 (31.54%), Postives = 66/130 (50.77%), Query Frame = 2

Query:  341 FAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF----------NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FAE  FL IF  E  IKI  LG       Y  + +N  D +VI     GS F           +F   V+ ALR LR  K+     SL+ ++ +L  +MK ++ +  L+   I++F+++G++ + G+F+F
Sbjct:  492 FAEFIFLGIFMSEMCIKIYGLG----TRPYFHSSFNCFDCIVIC----GSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNF 613

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007955.1 (pep scaffold:Pmarinus_7.0:GL476358:329878:417380:1 gene:ENSPMAG00000007100.1 transcript:ENSPMAT00000007955.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.1225 bits (222), Expect = 2.327e-21
Identity = 47/123 (38.21%), Postives = 72/123 (58.54%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            K  E  F  I+T E+++KI+A GF L+  T+LR+PWN LDF VIV+  +    +  +   +   R LR LK I  I  L+ ++ AL QA+K L  + IL +F + +F++IGL+ + G     C
Sbjct:  157 KNVEYTFTGIYTFESMVKILARGFCLHPFTFLRDPWNWLDFCVIVMAYVTEFIDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQAVKQLSDVMILTVFCLSVFALIGLQLFMGNLRHKC 279

HSP 2 Score: 28.4906 bits (62), Expect = 2.327e-21
Identity = 14/52 (26.92%), Postives = 28/52 (53.85%), Query Frame = 3

Query:  153 FHINPSRSLFIFSEDNLIRKIAQFVINLS----VVEIFVQLIILTNCIVLAL 296
            F  + + +L+I +  N +R+ A  ++  S    +  IF+   ILTNC+ + +
Sbjct:   98 FRFSATPALYILTPFNPVRRYAIKILTHSWQRTLFSIFIMFTILTNCVFMTM 149

HSP 3 Score: 77.411 bits (189), Expect = 2.104e-16
Identity = 43/139 (30.94%), Postives = 79/139 (56.83%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIG--STFNNFDQ----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEP 736
            ++A+  F  +F +E ++K +A GF      Y  N W  LDFL++ I I+   +T   +DQ    + +  LRALRPL+ +   + +++V+ AL  A+  ++ + ++ +   L+FSI+G+  +SGK+++ C NK      P
Sbjct: 1237 EYADKVFTYVFILEMLLKWVAYGF----QKYFTNAWCWLDFLIVDISIVSLMATALGYDQLSAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFSGKYYY-CLNKTDGQRLP 1370

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002291.1 (pep scaffold:Pmarinus_7.0:GL476946:177790:226078:1 gene:ENSPMAG00000002078.1 transcript:ENSPMAT00000002291.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.4185 bits (202), Expect = 9.112e-21
Identity = 44/128 (34.38%), Postives = 70/128 (54.69%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            I+ + K  E  F  I+  EA++KI+A GF     TYLR+PWN LD  V+VI    S     +   +   R +R LK I  +  L+ +++AL Q++K L  + IL +F + +F++I L+ + G     C
Sbjct:  148 INTELKVVEYVFTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSIPGFANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKC 275

HSP 2 Score: 33.8834 bits (76), Expect = 9.112e-21
Identity = 15/48 (31.25%), Postives = 29/48 (60.42%), Query Frame = 3

Query:  153 FHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLAL 296
            F  N  ++L +F+  N IR+I+  ++  ++  I +   ILTNC+ +A+
Sbjct:  101 FRFNACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCVFMAI 148

HSP 3 Score: 52.7582 bits (125), Expect = 3.598e-8
Identity = 35/117 (29.91%), Postives = 60/117 (51.28%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------GSTFNNFDQ----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLE 676
            F+ IFT E V+K+ AL     +  +   PWNV DF V+ + +        S  +N  +    RV+   R  R L+LI   + ++ ++ AL  ++  L  IG+L+  V+ +FS+ G+ 
Sbjct: 1310 FIGIFTTECVLKMFAL-----RQYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMS 1421

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002303.1 (pep scaffold:Pmarinus_7.0:GL476946:177796:226078:1 gene:ENSPMAG00000002078.1 transcript:ENSPMAT00000002303.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.4185 bits (202), Expect = 2.154e-20
Identity = 44/128 (34.38%), Postives = 70/128 (54.69%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            I+ + K  E  F  I+  EA++KI+A GF     TYLR+PWN LD  V+VI    S     +   +   R +R LK I  +  L+ +++AL Q++K L  + IL +F + +F++I L+ + G     C
Sbjct:  146 INTELKVVEYVFTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSIPGFANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKC 273

HSP 2 Score: 32.7278 bits (73), Expect = 2.154e-20
Identity = 15/48 (31.25%), Postives = 29/48 (60.42%), Query Frame = 3

Query:  153 FHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLAL 296
            F  N  ++L +F+  N IR+I+  ++  ++  I +   ILTNC+ +A+
Sbjct:   99 FRFNACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCVFMAI 146

HSP 3 Score: 52.7582 bits (125), Expect = 3.715e-8
Identity = 35/117 (29.91%), Postives = 60/117 (51.28%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII------GSTFNNFDQ----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLE 676
            F+ IFT E V+K+ AL     +  +   PWNV DF V+ + +        S  +N  +    RV+   R  R L+LI   + ++ ++ AL  ++  L  IG+L+  V+ +FS+ G+ 
Sbjct: 1393 FIGIFTTECVLKMFAL-----RQYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMS 1504

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000617.1 (pep scaffold:Pmarinus_7.0:GL477322:572:57208:-1 gene:ENSPMAG00000000554.1 transcript:ENSPMAT00000000617.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 86.6557 bits (213), Expect = 1.335e-19
Identity = 45/117 (38.46%), Postives = 72/117 (61.54%), Query Frame = 2

Query:  362 TIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRV--IGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            TI+ +E VIK++A GF + Q T+LR+PWN LD LV++  I+  T+ N  Q++  +   R LR LK I     L+ ++KAL Q++K L  + IL +F + +F++IG + + G     C
Sbjct:  171 TIYMMEVVIKVLARGFFMGQFTFLRDPWNWLDLLVVIFTIV--TYVNPKQKMSSLKTFRVLRVLKTISVFPGLKTIVKALIQSVKKLFDVIILTIFCLSIFALIGQQLFMGNLKNKC 285

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008004.1 (pep scaffold:Pmarinus_7.0:GL477228:88466:118438:1 gene:ENSPMAG00000007222.1 transcript:ENSPMAT00000008004.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.8702 bits (185), Expect = 6.746e-16
Identity = 42/117 (35.90%), Postives = 71/117 (60.68%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGC 706
            F  I+T E +IK +A GFV +  +YLRN WN LDF+VI++  +   F       + ALRA R LK+I  + +L+ ++ A+F++   LL + +L++F +++ S+ GL+ + G     C
Sbjct:    3 FTAIYTGEFLIKTLARGFVFHPYSYLRNAWNWLDFIVIILAYMTIAFPFLPG--LSALRAFRALKMIAILPALKTIVGAIFKSAGLLLNVMLLMIFALIIISLFGLQIFRGVLRQKC 117

HSP 2 Score: 61.2326 bits (147), Expect = 6.776e-11
Identity = 37/131 (28.24%), Postives = 73/131 (55.73%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLD-FLVIV--IGIIGSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKE 718
            ++ +++++  F++E  +K+I LG     + Y  + W +LD FLV+V  +  + S        R +  LRALRPL+ I   K +++V+ AL  ++  +  + ++ +   ++F I G++ + GKF F C + E
Sbjct: 1056 RYMDIFYMAFFSMEMFLKMIGLGL----HKYFTSFWTLLDCFLVLVSWLNQLASAVPALKSLRSLRTLRALRPLRAISRWKGMRLVVNALVTSIPSISNVMLMCLLFWMVFGIAGVQLFGGKF-FRCIDAE 1181

HSP 3 Score: 54.6842 bits (130), Expect = 9.487e-9
Identity = 37/133 (27.82%), Postives = 66/133 (49.62%), Query Frame = 2

Query:  362 TIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII-----GSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLN 745
            TIF  EAV+K+ AL        Y    WN+ DF V++  ++       +  N +  +I   R LR  KL    K+L ++I+ +  A+  +  + ++   VI +F++IG++   G+ ++   NK  S   P+ N
Sbjct:  607 TIFIAEAVLKLTALF----PRVYFHLGWNIFDFCVVITSVVEWILDSMSNTNINISIIRTFRILRVFKLAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLI-GRDYYHNRNKFSSGQVPRWN 734

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Yeast
Match: CCH1 (Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function [Source:SGD;Acc:S000003449])

HSP 1 Score: 65.4698 bits (158), Expect = 1.490e-12
Identity = 37/117 (31.62%), Postives = 66/117 (56.41%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIV---IGIIGSTFNNFD-QRVIGALRALRPLK-LIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGK 691
            F+  F+IE ++K +A GF+ + N YLRNPWN +DF V++   I +I    NN +  R+   L ALR L+ L  +  + Q     +F  +  + + G++ + ++  F++ GL  + G+
Sbjct: 1249 FIGAFSIEFIVKTVADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAYLKNNGNLSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGR 1365

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO46991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU9])

HSP 1 Score: 129.798 bits (325), Expect = 5.124e-34
Identity = 61/147 (41.50%), Postives = 103/147 (70.07%), Query Frame = 2

Query:  323 ISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIG-----STFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK---EPSNDEPQ 739
            I+++ + AE  FL IFT+E+V+KIIA GFV++   YLRN WN+LDF+++V+G+        T ++FD + + A R LRPL+L+  + SLQ+V+ ++ +A+ PL  I +L++FV+++++IIG+E + GK H  C++    +P+ DEP 
Sbjct:  105 INEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTFDEPH 251

HSP 2 Score: 82.4185 bits (202), Expect = 5.165e-19
Identity = 43/129 (33.33%), Postives = 77/129 (59.69%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKE 718
            + +F  +FTIE  IKIIA G +L++ ++ R+ +N+LDFLV+ + I+     +        R++  LR LRPL+ I   K L+ V++ +F A+K +  I ++ +    +F++IG++ + G F F C + E
Sbjct:  870 DYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGTF-FSCTDAE 997

HSP 3 Score: 28.8758 bits (63), Expect = 5.165e-19
Identity = 20/70 (28.57%), Postives = 34/70 (48.57%), Query Frame = 3

Query:   90 NKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            N+++ES+   +   +    P   + P  SLFIFS  N  R +   +   S    F+ L+I+ +  +LA E
Sbjct:  787 NRDHESVRSATSTEVMDHEP---MPPESSLFIFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAE 853

HSP 4 Score: 54.6842 bits (130), Expect = 1.656e-8
Identity = 40/131 (30.53%), Postives = 73/131 (55.73%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII--GSTFNNFDQRVIG-----ALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSN 727
            FL +FT+E V+K+  LGF    + Y  + +N  D LV++  ++  G T    DQR IG      +R LR  K+     SL  ++ +L  +M+ ++ + +L+   +++FS++G++ + GKF+ G  +   SN
Sbjct:  497 FLALFTLEMVVKMYCLGF----HGYFASLFNRFDCLVVISSLLELGLT-EAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKFNLGDEDVPRSN 622

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO36844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI0])

HSP 1 Score: 93.5893 bits (231), Expect = 1.738e-23
Identity = 48/124 (38.71%), Postives = 77/124 (62.10%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG----IIGSTFNNFDQ-----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            FL IFTIE ++K+IALG  +  + YLR+ WNV+D  ++VI     I+ +T N  +      RV  ALR LRPL++I     L+IV++ L  ++KP+  I ++     ++F I+G++ + GKFH+
Sbjct:  972 FLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFHY 1095

HSP 2 Score: 32.7278 bits (73), Expect = 1.738e-23
Identity = 20/59 (33.90%), Postives = 32/59 (54.24%), Query Frame = 3

Query:  129 TLDISLPNFHINPSR--SLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
            T+DI +P    +     SL++F+ DN  R + + +      +  V L IL NC+V+ALE
Sbjct:  893 TVDIKVPTSCCSKRADWSLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALE 951

HSP 3 Score: 65.855 bits (159), Expect = 3.087e-12
Identity = 35/124 (28.23%), Postives = 65/124 (52.42%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ---------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K A   F  +F +EA++KI ALG       Y+++ WN LD L++++ I+G                 RV+  LR  R LKL+   + ++ ++  + QA+  +  +G+L + +  +FS +G+E +
Sbjct: 1280 KCANYVFTAVFILEAILKIFALGI----KRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELF 1399

HSP 4 Score: 55.4546 bits (132), Expect = 8.426e-9
Identity = 35/119 (29.41%), Postives = 64/119 (53.78%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ--RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMF--SIIGLEFYSGKFHF 700
            F  IF IE ++K+I LGF      Y+++ +N+ D  +++I ++   F + D    V+ + R LR  KL+  + +L+  +  +   M  ++    L + VI MF  SI+G+  + GK+ F
Sbjct:  450 FTAIFGIEMIMKLIGLGFY----GYIKDAFNIFDGTIVIISVV-ELFGDDDSGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTF--LALLVIFMFTASILGMNLFGGKYRF 561

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO35497 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLD1])

HSP 1 Score: 85.1149 bits (209), Expect = 6.880e-19
Identity = 50/126 (39.68%), Postives = 79/126 (62.70%), Query Frame = 2

Query:  350 LYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLD-FLVIV--IGIIGSTFNNFDQRVIG------ALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            L F  IFTIE +IK++A+G VL   TYLR+ W+VLD FLV+V  I II +  ++    V+G      ALR LRPL++I     L++V++ L  ++KP+    ++     +MF I+G++ + GKF++
Sbjct:   58 LVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYY 183

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO35661 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKR1])

HSP 1 Score: 82.4185 bits (202), Expect = 7.082e-18
Identity = 44/127 (34.65%), Postives = 72/127 (56.69%), Query Frame = 2

Query:  347 ELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSN 727
            E  F  I+TIE   KIIA GF L++  YLR+ WN LDF+V+++G +  + +  +   I   R  R L+ I  +K L+ ++  L  +MK L  + +L +F I +F++IG++ + G+    C    P N
Sbjct:   77 EYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTISPDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGELRNKCALPVPEN 203

HSP 2 Score: 78.9518 bits (193), Expect = 1.089e-16
Identity = 44/120 (36.67%), Postives = 73/120 (60.83%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI---IGSTFNNFDQ----RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKF 694
            F  IFT+E ++K I LGF     TY  NPWN+LDF+++++ I   + + F N DQ    R +  LRA RPL+ I   + +++VI +L  A+  +  + ++ +   L+FSI+G++ + GKF
Sbjct:  868 FAVIFTVEMLLKWIGLGF----KTYFTNPWNILDFVIVIVSIMVSLATIFGN-DQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKF 982

HSP 3 Score: 50.8322 bits (120), Expect = 2.593e-7
Identity = 30/120 (25.00%), Postives = 64/120 (53.33%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNF-------DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKF 694
            F  IF +E ++K+IALGF+     Y+R  WN+ D +V++I I+    N F          V+   R LR LKL  +  ++  ++  + +++  L  + +++  ++ +F+++G++     +
Sbjct:  542 FTFIFLLEMILKLIALGFL----GYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSY 657

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO46797 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RP56])

HSP 1 Score: 77.0258 bits (188), Expect = 4.898e-16
Identity = 45/155 (29.03%), Postives = 80/155 (51.61%), Query Frame = 2

Query:  290 GLGSPPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPA 754
            GL  PP            AE  F  I+T+E + KII+ GFV +   YLR+ WN LD LV+ +  +    +      I  LR LR  + I  +K L+ ++ +L +++K L  + +L +F + + ++IGL+ ++G+    C  +  + +   +N P+
Sbjct:   79 GLNDPPEE----------AEYIFTAIYTLEMICKIISRGFVKDSYAYLRDTWNWLDSLVVFLSYLSLAPDIASLSGIRTLRVLRAFRTISALKGLRAMVNSLLRSLKLLTDVLVLFLFSLCVLALIGLQLFTGELRNKCVLE--TANATTINNPS 221

HSP 2 Score: 74.7146 bits (182), Expect = 3.240e-15
Identity = 45/139 (32.37%), Postives = 76/139 (54.68%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGI-IGSTFNNFDQRVIGA---LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQL 742
             F    F  IF++E ++K+I  GFV    +Y  + WN LD L++ + + +   F N +  V  +   +R LRPL+ I  +  +++VI ALF A+  +  + ++ M   LMFSI G++ ++GKF + C N E     P +
Sbjct:  828 NFLNFLFAAIFSLEFILKVIGFGFV----SYFTSVWNCLDALIVSVCVSVACLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKF-YKCVNAEDEKLSPSV 961

HSP 3 Score: 52.7582 bits (125), Expect = 5.893e-8
Identity = 35/126 (27.78%), Postives = 62/126 (49.21%), Query Frame = 2

Query:  365 IFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ---------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNK 715
            IF +EA+IKIIA+        Y +  W++ DF+V+V  I+G       +         RV+   R  R L+++   K ++ ++ A+  +M  L  IG L+  V+ +++IIG+  +      G  N 
Sbjct: 1151 IFLLEAIIKIIAMRL-----HYFKKAWSIFDFVVVVSSIVGMYLGMSTESAVVSPGLLRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFGNIKKQGALND 1271

HSP 4 Score: 47.3654 bits (111), Expect = 3.317e-6
Identity = 28/122 (22.95%), Postives = 60/122 (49.18%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTF-NNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKE 718
            F  +F +E ++KI A G V     Y+ N WN+ D ++I+   +      +    +   LR LR L L  + +++  +I+A+  ++ P+  I  ++  +I +F+++G+  +   +    F   
Sbjct:  519 FTGLFILEMIVKIFAFGLV----KYICNKWNLFDSVIIITSAVAYFLGTSTGVSIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHYTAEAFGSR 636

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1aa (voltage-dependent P/Q-type calcium channel subunit alpha-1A [Source:NCBI gene;Acc:101170273])

HSP 1 Score: 157.918 bits (398), Expect = 3.812e-48
Identity = 76/155 (49.03%), Postives = 107/155 (69.03%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRP 766
            P   +  +S++    E YF+ IF  E+ IKI+ALGF  ++N+YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF+     DE +   P    P
Sbjct:  128 PDGDKTPLSERLDDTEPYFIGIFCFESGIKILALGFAFHKNSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYIGKFHSTCFDNH--TDEIREEFPCGTEP 280

HSP 2 Score: 52.7582 bits (125), Expect = 3.812e-48
Identity = 27/56 (48.21%), Postives = 35/56 (62.50%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSV---VEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN +RK A+ +            +   I+ NCIVLALE H P+GDK+
Sbjct:   78 NRSLFIFSEDNFVRKYAKKITEWPYPLPSSWMILATIIANCIVLALEQHLPDGDKT 133

HSP 3 Score: 83.1889 bits (204), Expect = 1.415e-17
Identity = 37/128 (28.91%), Postives = 73/128 (57.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG VL+Q +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F+++ ++ + G+F +
Sbjct: 1204 RYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGNSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYLLFMFIFAVVAVQLFKGRFFY 1331

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ab (calcium voltage-gated channel subunit alpha1 A [Source:NCBI gene;Acc:105357443])

HSP 1 Score: 154.451 bits (389), Expect = 4.497e-48
Identity = 75/156 (48.08%), Postives = 106/156 (67.95%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P   +  +S++ +  E YF+ IF  E+ IKI+ALGF L++ +YLRN WNV+DF+V++ GI+ S  +  D R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+   ILMF+IIGLEFY GKFH  C+++          E   KRPC
Sbjct:  123 PDGDKTPLSERLEETEPYFIAIFCFESGIKILALGFALHKGSYLRNGWNVMDFVVVLTGILSSVGSQLDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFVAILMFAIIGLEFYMGKFHKTCYDE-------ITKEIVDKRPC 271

HSP 2 Score: 55.8398 bits (133), Expect = 4.497e-48
Identity = 27/54 (50.00%), Postives = 36/54 (66.67%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSV-VEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLF+FSEDN +RK A+ +       E  +   I+ NCIVLALE H P+GDK+
Sbjct:   75 NRSLFLFSEDNFVRKYAKKITECCTPFEWMILTTIIANCIVLALEQHLPDGDKT 128

HSP 3 Score: 82.4185 bits (202), Expect = 2.694e-17
Identity = 36/128 (28.12%), Postives = 72/128 (56.25%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG  L++ +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F+++ ++ + G+F +
Sbjct: 1131 RYFDYVFTGVFTFEMLIKMVVLGLFLHEGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFY 1258

HSP 4 Score: 60.4622 bits (145), Expect = 5.701e-10
Identity = 36/121 (29.75%), Postives = 68/121 (56.20%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIG-----IIGSTFNNF-DQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F   F +E+V+K+IA G +     Y R+ WNV DF V VIG     ++   ++NF +   +   RA R +KL+   ++++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1452 KYLNIVFTIFFFMESVLKVIAFGPL----NYFRDAWNVFDF-VSVIGSITDILVTEFWDNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLF 1567

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ba (voltage-dependent N-type calcium channel subunit alpha-1B [Source:NCBI gene;Acc:101157063])

HSP 1 Score: 157.532 bits (397), Expect = 1.255e-47
Identity = 78/156 (50.00%), Postives = 107/156 (68.59%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRPC 769
            P + +  +S++    E YF+ IF  EA IKIIALGF  ++ +YLRN WNV+DF+V++ GI+ +   +FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGL+FY GKFH  CF     ND    +E A   PC
Sbjct:  112 PASDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFR----NDT---DEQAADFPC 260

HSP 2 Score: 51.6026 bits (122), Expect = 1.255e-47
Identity = 28/54 (51.85%), Postives = 36/54 (66.67%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVINLSVVEIFVQL-IILTNCIVLALEVHQPEGDKS 326
            +RSLFIFSEDN+IRK A+ +        ++ L  I+ NCIVLALE H P  DK+
Sbjct:   64 NRSLFIFSEDNIIRKYAKKITEWPYPFGYMILATIIANCIVLALEQHLPASDKT 117

HSP 3 Score: 83.1889 bits (204), Expect = 1.219e-17
Identity = 40/127 (31.50%), Postives = 72/127 (56.69%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGA------LRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK++ LG +L+  +Y R+ WN+LDF+V+   ++   F+    + I        LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F++I ++ + GKF +
Sbjct: 1107 KYLDYAFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGNKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFY 1233

HSP 4 Score: 48.1358 bits (113), Expect = 5.854e-6
Identity = 26/92 (28.26%), Postives = 51/92 (55.43%), Query Frame = 2

Query:  350 LYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIG-----ALRALRPLKLIYNIKSLQIVIKALFQAMK 610
            + F T+F++E ++KIIA G +    +YL++ WNV DF+ ++  I     +  + R++        RA R +KL+    +++I++    Q+ K
Sbjct: 1431 IVFTTLFSLECILKIIAFGPL----SYLKDAWNVFDFVTVLGSITDILVSEINDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFK 1518

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1bb (voltage-dependent N-type calcium channel subunit alpha-1B [Source:NCBI gene;Acc:101167885])

HSP 1 Score: 155.992 bits (393), Expect = 7.312e-47
Identity = 70/139 (50.36%), Postives = 102/139 (73.38%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKE 718
            P + +  +S++    E YF+ IF  EA IKIIALGF  ++ +YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F I+MF+IIG+EFY G+FH  CF  +
Sbjct:  116 PASDKTPMSERLDDTEPYFIGIFCFEAAIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGEFHKTCFKSD 254

HSP 2 Score: 50.447 bits (119), Expect = 7.312e-47
Identity = 28/54 (51.85%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  168 SRSLFIFSEDNLIRKIAQFVIN-LSVVEIFVQLIILTNCIVLALEVHQPEGDKS 326
            +RSLFIF EDNLIRK    ++N  +  E  +   I+ NCIVLALE H P  DK+
Sbjct:   70 NRSLFIFREDNLIRKY--LLLNPHTPFEYMILATIIANCIVLALEQHLPASDKT 121

HSP 3 Score: 83.5741 bits (205), Expect = 4.392e-22
Identity = 43/129 (33.33%), Postives = 71/129 (55.04%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVI--------VIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            K+ +  F  +FT E VIK+I LG +L+  +Y R+ WN+LDF+V+          G  G   N    + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ M  + +F++I ++ + GKF +
Sbjct: 1170 KYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTI--KSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFY 1296

HSP 4 Score: 39.6614 bits (91), Expect = 4.392e-22
Identity = 17/48 (35.42%), Postives = 29/48 (60.42%), Query Frame = 3

Query:  156 HINPSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALE 299
             I P  S+FIF + N +R++  +V+NL   E+ + ++I  + I LA E
Sbjct: 1109 QILPYSSMFIFGQTNPLRRLCHYVVNLRYFEMCILMVITMSSITLAAE 1156

HSP 5 Score: 67.781 bits (164), Expect = 1.975e-12
Identity = 35/120 (29.17%), Postives = 65/120 (54.17%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIG-----ALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            K+  + F  +FT+E ++KIIA     N   YL+ PWNV DF+ ++  I    F   + ++I        RA R +KL+    +++I++    Q+ K L  + +LI  +  +++IIG++ +
Sbjct: 1490 KYLNIVFTGLFTLECILKIIAF----NPLNYLKEPWNVFDFVTVIGSITDILFTEINNKMINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF 1605

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1aa (voltage-dependent P/Q-type calcium channel subunit alpha-1A [Source:NCBI gene;Acc:101170273])

HSP 1 Score: 158.303 bits (399), Expect = 1.843e-43
Identity = 76/155 (49.03%), Postives = 107/155 (69.03%), Query Frame = 2

Query:  302 PPAGR*QISKK*KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKRP 766
            P   +  +S++    E YF+ IF  E+ IKI+ALGF  ++N+YLRN WNV+DF+V++ GI+ +  ++FD R + A+R LRPLKL+  I SLQ+V+K++ +AM PLLQIG+L+ F ILMF+IIGLEFY GKFH  CF+     DE +   P    P
Sbjct:   20 PDGDKTPLSERLDDTEPYFIGIFCFESGIKILALGFAFHKNSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYIGKFHSTCFDNH--TDEIREEFPCGTEP 172

HSP 2 Score: 83.1889 bits (204), Expect = 1.182e-17
Identity = 37/128 (28.91%), Postives = 73/128 (57.03%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQ-------RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHF 700
            ++ +  F  +FT E +IK++ LG VL+Q +Y R+ WN+LDF+V+   ++   F    +       + +  LR LRPLK I  +  L+ V   +  ++K +L I I+ +  + +F+++ ++ + G+F +
Sbjct: 1121 RYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGNSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYLLFMFIFAVVAVQLFKGRFFY 1248

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.450e-42
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKR--PC 769
            +  E YFL IF +EA++KI+ALGF  ++  YLRN WN++DF+V+V G+I     +FDQR + ALR LRPLKL+   +SLQ+V+K++ +AM PLLQIG+L++F I +F+IIGLEFYSG FH+ CF  + ++D+  L  P  K   PC
Sbjct:   18 ELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPSFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCF--KANSDKLPLPIPTEKSLVPC 161

HSP 2 Score: 72.4034 bits (176), Expect = 1.148e-19
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII----------------GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            +F +  F  +FT+E ++KII LG +L+  +Y R+ WN+LD  V++  ++                GS+  N +  + +  LR LRPLK I  +  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   S+ E
Sbjct:  878 EFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKF-FYCNDASKSSRE 1025

HSP 3 Score: 42.3578 bits (98), Expect = 1.148e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLR 332
            P  ++FIFS  N +R+   FV+NL   ++F+ ++I  + I LA E   P  +KSL+
Sbjct:  820 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVKEKSLK 873

HSP 4 Score: 56.225 bits (134), Expect = 1.102e-8
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGAL------RALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F  +F+IE V+KI+A G       Y R+PWN+ DF+ ++  I     +        +L      RA R +KL+    +++I++    Q+ K L  + +LI  +  ++SIIG++ +
Sbjct: 1232 FTVMFSIECVLKIMAFG----PKNYFRDPWNIFDFITVIGSITDVLVSELQDSSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVF 1342

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.474e-42
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKR--PC 769
            +  E YFL IF +EA++KI+ALGF  ++  YLRN WN++DF+V+V G+I     +FDQR + ALR LRPLKL+   +SLQ+V+K++ +AM PLLQIG+L++F I +F+IIGLEFYSG FH+ CF  + ++D+  L  P  K   PC
Sbjct:   55 ELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPSFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCF--KANSDKLPLPIPTEKSLVPC 198

HSP 2 Score: 72.4034 bits (176), Expect = 1.226e-19
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII----------------GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            +F +  F  +FT+E ++KII LG +L+  +Y R+ WN+LD  V++  ++                GS+  N +  + +  LR LRPLK I  +  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   S+ E
Sbjct:  842 EFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKF-FYCNDASKSSRE 989

HSP 3 Score: 41.9726 bits (97), Expect = 1.226e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLR 332
            P  ++FIFS  N +R+   FV+NL   ++F+ ++I  + I LA E   P  +KSL+
Sbjct:  784 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVKEKSLK 837

HSP 4 Score: 56.225 bits (134), Expect = 1.110e-8
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGAL------RALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F  +F+IE V+KI+A G       Y R+PWN+ DF+ ++  I     +        +L      RA R +KL+    +++I++    Q+ K L  + +LI  +  ++SIIG++ +
Sbjct: 1196 FTVMFSIECVLKIMAFG----PKNYFRDPWNIFDFITVIGSITDVLVSELQDSSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVF 1306

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.563e-42
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKR--PC 769
            +  E YFL IF +EA++KI+ALGF  ++  YLRN WN++DF+V+V G+I     +FDQR + ALR LRPLKL+   +SLQ+V+K++ +AM PLLQIG+L++F I +F+IIGLEFYSG FH+ CF  + ++D+  L  P  K   PC
Sbjct:   18 ELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPSFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCF--KANSDKLPLPIPTEKSLVPC 161

HSP 2 Score: 72.4034 bits (176), Expect = 1.267e-19
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII----------------GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            +F +  F  +FT+E ++KII LG +L+  +Y R+ WN+LD  V++  ++                GS+  N +  + +  LR LRPLK I  +  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   S+ E
Sbjct:  847 EFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKF-FYCNDASKSSRE 994

HSP 3 Score: 41.9726 bits (97), Expect = 1.267e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLR 332
            P  ++FIFS  N +R+   FV+NL   ++F+ ++I  + I LA E   P  +KSL+
Sbjct:  789 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVKEKSLK 842

HSP 4 Score: 56.225 bits (134), Expect = 1.111e-8
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGAL------RALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F  +F+IE V+KI+A G       Y R+PWN+ DF+ ++  I     +        +L      RA R +KL+    +++I++    Q+ K L  + +LI  +  ++SIIG++ +
Sbjct: 1201 FTVMFSIECVLKIMAFG----PKNYFRDPWNIFDFITVIGSITDVLVSELQDSSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVF 1311

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.609e-42
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKR--PC 769
            +  E YFL IF +EA++KI+ALGF  ++  YLRN WN++DF+V+V G+I     +FDQR + ALR LRPLKL+   +SLQ+V+K++ +AM PLLQIG+L++F I +F+IIGLEFYSG FH+ CF  + ++D+  L  P  K   PC
Sbjct:   55 ELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPSFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCF--KANSDKLPLPIPTEKSLVPC 198

HSP 2 Score: 72.4034 bits (176), Expect = 1.236e-19
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII----------------GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            +F +  F  +FT+E ++KII LG +L+  +Y R+ WN+LD  V++  ++                GS+  N +  + +  LR LRPLK I  +  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   S+ E
Sbjct:  842 EFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKF-FYCNDASKSSRE 989

HSP 3 Score: 42.3578 bits (98), Expect = 1.236e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLR 332
            P  ++FIFS  N +R+   FV+NL   ++F+ ++I  + I LA E   P  +KSL+
Sbjct:  784 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVKEKSLK 837

HSP 4 Score: 56.225 bits (134), Expect = 1.111e-8
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGAL------RALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F  +F+IE V+KI+A G       Y R+PWN+ DF+ ++  I     +        +L      RA R +KL+    +++I++    Q+ K L  + +LI  +  ++SIIG++ +
Sbjct: 1196 FTVMFSIECVLKIMAFG----PKNYFRDPWNIFDFITVIGSITDVLVSELQDSSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVF 1306

[Close]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.622e-42
Identity = 75/146 (51.37%), Postives = 106/146 (72.60%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDEPQLNEPAIKR--PC 769
            +  E YFL IF +EA++KI+ALGF  ++  YLRN WN++DF+V+V G+I     +FDQR + ALR LRPLKL+   +SLQ+V+K++ +AM PLLQIG+L++F I +F+IIGLEFYSG FH+ CF  + ++D+  L  P  K   PC
Sbjct:   32 ELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPSFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCF--KANSDKLPLPIPTEKSLVPC 175

HSP 2 Score: 72.4034 bits (176), Expect = 1.247e-19
Identity = 43/149 (28.86%), Postives = 78/149 (52.35%), Query Frame = 2

Query:  338 KFAELYFLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGII----------------GSTFNNFDQ-RVIGALRALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFYSGKFHFGCFNKEPSNDE 733
            +F +  F  +FT+E ++KII LG +L+  +Y R+ WN+LD  V++  ++                GS+  N +  + +  LR LRPLK I  +  L+ V   +  ++K +  I I+ +    +F++I ++ + GKF F C +   S+ E
Sbjct:  819 EFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKF-FYCNDASKSSRE 966

HSP 3 Score: 41.9726 bits (97), Expect = 1.247e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  165 PSRSLFIFSEDNLIRKIAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLR 332
            P  ++FIFS  N +R+   FV+NL   ++F+ ++I  + I LA E   P  +KSL+
Sbjct:  761 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAE--DPVKEKSLK 814

HSP 4 Score: 55.8398 bits (133), Expect = 1.183e-8
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2

Query:  356 FLTIFTIEAVIKIIALGFVLNQNTYLRNPWNVLDFLVIVIGIIGSTFNNFDQRVIGAL------RALRPLKLIYNIKSLQIVIKALFQAMKPLLQIGILIMFVILMFSIIGLEFY 682
            F  +F+IE V+KI+A G       Y R+PWN+ DF+ ++  I     +        +L      RA R +KL+    +++I++    Q+ K L  + +LI  +  ++SIIG++ +
Sbjct: 1173 FTVMFSIECVLKIMAFG----PKNYFRDPWNIFDFITVIGSITDVLVSELQDSSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVF 1283

The following BLAST results are available for this feature:

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Yeast
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CACNA1B	1.071e-51	52.35	calcium voltage-gated channel subunit alpha1 B [So...	[more]
CACNA1B	1.071e-51	52.35	calcium voltage-gated channel subunit alpha1 B [So...	[more]
CACNA1B	1.071e-51	52.35	calcium voltage-gated channel subunit alpha1 B [So...	[more]
CACNA1B	1.071e-51	52.35	calcium voltage-gated channel subunit alpha1 B [So...	[more]
CACNA1B	1.690e-51	52.35	calcium voltage-gated channel subunit alpha1 B [So...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-2	3.553e-41	47.41	High voltage activated calcium channel alpha-1 sub...	[more]
unc-2	4.333e-41	47.41	High voltage activated calcium channel alpha-1 sub...	[more]
unc-2	4.987e-41	47.41	High voltage activated calcium channel alpha-1 sub...	[more]
unc-2	5.933e-41	47.41	High voltage activated calcium channel alpha-1 sub...	[more]
unc-2	9.505e-41	47.41	High voltage activated calcium channel alpha-1 sub...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cac	7.197e-45	46.71	gene:FBgn0263111 transcript:FBtr0474241	[more]
cac	1.135e-44	46.71	gene:FBgn0263111 transcript:FBtr0307323	[more]
cac	1.233e-44	46.71	gene:FBgn0263111 transcript:FBtr0307325	[more]
cac	1.634e-44	46.71	gene:FBgn0263111 transcript:FBtr0332391	[more]
cac	3.167e-44	45.28	gene:FBgn0263111 transcript:FBtr0307320	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cacna1aa	5.039e-49	52.17	calcium channel, voltage-dependent, P/Q type, alph...	[more]
cacna1aa	5.166e-49	52.17	calcium channel, voltage-dependent, P/Q type, alph...	[more]
cacna1ab	9.362e-49	51.82	calcium channel, voltage-dependent, P/Q type, alph...	[more]
cacna1ab	1.017e-48	51.82	calcium channel, voltage-dependent, P/Q type, alph...	[more]
cacna1bb	1.198e-47	49.36	calcium channel, voltage-dependent, N type, alpha ...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000009772.1	1.313e-45	51.05	pep primary_assembly:Xenopus_tropicalis_v9.1:KV460...	[more]
mrps11	9.598e-43	49.67	mitochondrial ribosomal protein S11 [Source:Xenbas...	[more]
mrps11	1.009e-42	49.67	mitochondrial ribosomal protein S11 [Source:Xenbas...	[more]
mrps11	1.392e-42	49.67	mitochondrial ribosomal protein S11 [Source:Xenbas...	[more]
mrps11	2.221e-41	45.66	mitochondrial ribosomal protein S11 [Source:Xenbas...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Cacna1b	3.984e-52	50.00	calcium channel, voltage-dependent, N type, alpha ...	[more]
Cacna1b	4.187e-52	50.00	calcium channel, voltage-dependent, N type, alpha ...	[more]
Cacna1b	4.221e-52	50.00	calcium channel, voltage-dependent, N type, alpha ...	[more]
Cacna1b	4.221e-52	50.00	calcium channel, voltage-dependent, N type, alpha ...	[more]
Cacna1b	4.702e-52	50.00	calcium channel, voltage-dependent, N type, alpha ...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P56698\|CAC1B_DIPOM	9.702e-52	50.64	Probable voltage-dependent N-type calcium channel ...	[more]
sp\|Q05152\|CAC1B_RABIT	1.945e-51	50.64	Voltage-dependent N-type calcium channel subunit a...	[more]
sp\|O55017\|CAC1B_MOUSE	2.413e-51	50.00	Voltage-dependent N-type calcium channel subunit a...	[more]
sp\|Q00975\|CAC1B_HUMAN	4.490e-51	52.35	Voltage-dependent N-type calcium channel subunit a...	[more]
sp\|Q02294\|CAC1B_RAT	4.341e-50	51.68	Voltage-dependent N-type calcium channel subunit a...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2I2V1V1	2.389e-49	51.28	Voltage-dependent N-type calcium channel subunit a...	[more]
A0A5F5XJ38	2.532e-49	51.28	Voltage-dependent N-type calcium channel subunit a...	[more]
A0A287APC1	2.842e-49	53.02	Voltage-dependent N-type calcium channel subunit a...	[more]
A0A286ZQ69	2.842e-49	53.02	Voltage-dependent N-type calcium channel subunit a...	[more]
P91744	3.219e-49	51.37	Voltage-dependent calcium channel OS=Heterololigo ...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cacna1bb	4.653e-50	51.02	calcium channel, voltage-dependent, N type, alpha ...	[more]
cacna1bb	4.810e-50	51.02	calcium channel, voltage-dependent, N type, alpha ...	[more]
cacna1bb	4.849e-50	51.02	calcium channel, voltage-dependent, N type, alpha ...	[more]
cacna1bb	5.583e-50	51.02	calcium channel, voltage-dependent, N type, alpha ...	[more]
cacna1aa	2.549e-46	51.45	calcium channel, voltage-dependent, P/Q type, alph...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000007955.1	2.327e-21	38.21	pep scaffold:Pmarinus_7.0:GL476358:329878:417380:1...	[more]
ENSPMAT00000002291.1	9.112e-21	34.38	pep scaffold:Pmarinus_7.0:GL476946:177790:226078:1...	[more]
ENSPMAT00000002303.1	2.154e-20	34.38	pep scaffold:Pmarinus_7.0:GL476946:177796:226078:1...	[more]
ENSPMAT00000000617.1	1.335e-19	38.46	pep scaffold:Pmarinus_7.0:GL477322:572:57208:-1 ge...	[more]
ENSPMAT00000008004.1	6.746e-16	35.90	pep scaffold:Pmarinus_7.0:GL477228:88466:118438:1 ...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
CCH1	1.490e-12	31.62	Voltage-gated high-affinity calcium channel; invol...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO46991	5.124e-34	41.50	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO36844	1.738e-23	38.71	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35497	6.880e-19	39.68	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35661	7.082e-18	34.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46797	4.898e-16	29.03	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cacna1aa	3.812e-48	49.03	voltage-dependent P/Q-type calcium channel subunit...	[more]
cacna1ab	4.497e-48	48.08	calcium voltage-gated channel subunit alpha1 A [So...	[more]
cacna1ba	1.255e-47	50.00	voltage-dependent N-type calcium channel subunit a...	[more]
cacna1bb	7.312e-47	50.36	voltage-dependent N-type calcium channel subunit a...	[more]
cacna1aa	1.843e-43	49.03	voltage-dependent P/Q-type calcium channel subunit...	[more]

BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000028279.1	1.450e-42	51.37	SMESG000028279.1	[more]
SMESG000028279.1	1.474e-42	51.37	SMESG000028279.1	[more]
SMESG000028279.1	1.563e-42	51.37	SMESG000028279.1	[more]
SMESG000028279.1	1.609e-42	51.37	SMESG000028279.1	[more]
SMESG000028279.1	1.622e-42	51.37	SMESG000028279.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30008999 ID=SMED30008999|Name=Voltage-dependent N-type calcium channel subunit alpha|organism=Schmidtea mediterranea sexual|type=transcript|length=794bp
TTTAAATTTAAATAATGAAGAATTTATGATATATTTGAAAATCAGTAAAT
ATTTTTTTTCAAATGTTTTTCGGAAACTCGTTTGTTGCGAATAAGAATTA
TGAGTCGATTTTCCAATTATCAAAAAATACTTTAGATATTTCTCTCCCGA
ATTTTCATATCAATCCTTCAAGATCTTTATTTATATTTTCCGAAGACAAT
TTAATTAGAAAAATTGCACAGTTTGTGATAAATTTGAGTGTGGTAGAAAT
TTTTGTACAATTAATAATTTTGACAAACTGCATTGTTTTGGCCTTGGAAG
TCCACCAGCCGGAAGGTGACAAATCTCTAAGAAATAAAAATTTGCCGAAC
TTTATTTTCTAACCATTTTTACCATTGAAGCAGTTATCAAAATAATTGCC
TTGGGTTTCGTGCTTAATCAAAATACTTATTTGCGCAATCCCTGGAATGT
CTTAGACTTCCTTGTAATAGTTATCGGAATAATAGGCTCAACGTTTAATA
ATTTTGACCAAAGAGTTATCGGAGCACTGAGAGCCTTGCGGCCTTTAAAA
CTTATTTACAACATAAAAAGCTTACAAATCGTCATCAAAGCCCTTTTCCA
AGCTATGAAGCCTTTATTGCAAATTGGAATTTTAATAATGTTTGTCATAT
TAATGTTTTCCATCATCGGCTTGGAGTTTTACAGCGGGAAATTTCATTTT
GGTTGTTTTAATAAAGAGCCGAGTAATGATGAGCCTCAATTGAATGAGCC
GGCAATAAAGAGGCCGTGTTCACCGAGCAGCAGCAGCAGCAGCA

protein sequence of SMED30008999-orf-1

>SMED30008999-orf-1 ID=SMED30008999-orf-1|Name=SMED30008999-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MFFGNSFVANKNYESIFQLSKNTLDISLPNFHINPSRSLFIFSEDNLIRK
IAQFVINLSVVEIFVQLIILTNCIVLALEVHQPEGDKSLRNKNLPNFIF*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte

Vocabulary: **INTERPRO**
Term	Definition
IPR005449	VDCC_R_a1su
IPR005821	Ion_trans_dom
IPR027359	Volt_channel_dom_sf

Vocabulary: **biological process**
Term	Definition
GO:0055085	transmembrane transport
GO:0006811	ion transport
GO:0070588	calcium ion transmembrane transport
GO:0086010	membrane depolarization during action potential
GO:0006810	transport
GO:0006816	calcium ion transport
GO:0007269	neurotransmitter secretion
GO:0007626	locomotory behavior
GO:0008016	regulation of heart contraction
GO:0008217	regulation of blood pressure
GO:0034765	regulation of ion transmembrane transport
GO:0048265	response to pain
GO:0051924	regulation of calcium ion transport

Vocabulary: **molecular function**
Term	Definition
GO:0005216	ion channel activity
GO:0000166	nucleotide binding
GO:0005244	voltage-gated ion channel activity
GO:0005245	voltage-gated calcium channel activity
GO:0005262	calcium channel activity
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0008331	high voltage-gated calcium channel activity
GO:0046872	metal ion binding

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane
GO:0098793	presynapse
GO:0005891	voltage-gated calcium channel complex
GO:0016021	integral component of membrane
GO:0030425	dendrite
GO:0043025	neuronal cell body

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005449	Voltage-dependent calcium channel, R-type, alpha-1 subunit	PANTHER	PTHR45628:SF5	VOLTAGE-DEPENDENT R-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1E	coord: 36..94
None	No IPR available	PANTHER	PTHR45628	FAMILY NOT NAMED	coord: 36..94
None	No IPR available	TMHMM	TMhelix		coord: 56..78