SMED30008995

Overview
NameSMED30008995
Smed IDSMED30008995
Length (bp)1968
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008995 (SMED30008995) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008995 (SMED30008995) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008995 (SMED30008995) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008995 (SMED30008995) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008995

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30008995h1SMcG0022051 dd_Smed_v4_8514_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008995 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000503:87072..108756 -8320
Homology
BLAST of SMED30008995 vs. TrEMBL
Match: W5N5P2 (WASP homolog associated with actin, golgi membranes and microtubules OS=Lepisosteus oculatus OX=7918 GN=WHAMM PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.327e-8
Identity = 66/303 (21.78%), Postives = 140/303 (46.20%), Query Frame = 3
Query:   18 LMFNDIYFNSYITFLTRILLDLSRKVSNFPITTKITSV---TIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEE-DKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENAT---YFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKE------SIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAE---HLNDYYAH-QRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIF 875
            + +N I     +T   R L    R+    P  T+ +     ++++ + +H +L+ +   + ++FP+   FE+  +        W    +  P+ +EE D DL    +  ++ Y    V+ICG+ ++ + LF ++      YFE+  EF  K   + ++  ++Q   +L +H   + +  L + +Y++ED   E YKE       +++       ++ E + ML  K+E +  + +  ++GP+ IE + +   E      D  +  Q I++N  +E SK+L  M    + +M  D+ ++    +
Sbjct:   48 VQWNVIECKFAVTCHNRTLQRQRRREEQAPADTQTSWAGLFSVSDLRNVHRQLTGVSDVLGAFFPDLSAFEVGNL--------WDLLFLGRPSGLEEPDMDLDWPCR-QLERYFSTAVDICGRTIVLDCLFAQDCRDVEEYFENLHEFKKKSMEDQVVRAREQLRKILQQHKAADKMVLLLK-IYEEED---EAYKELVTVATQLYQYLLQPFRDMRE-LAMLC-KME-IMKSLEFEDLGPKRIEALEKEAEEWRKRAQDAVSSIQDITVNYFVETSKALAGM----LKQMEEDRQRFGPAAW 330          
BLAST of SMED30008995 vs. TrEMBL
Match: W5N5P9 (WASP homolog associated with actin, golgi membranes and microtubules OS=Lepisosteus oculatus OX=7918 GN=WHAMM PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.401e-8
Identity = 66/303 (21.78%), Postives = 140/303 (46.20%), Query Frame = 3
Query:   18 LMFNDIYFNSYITFLTRILLDLSRKVSNFPITTKITSV---TIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEE-DKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENAT---YFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKE------SIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAE---HLNDYYAH-QRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIF 875
            + +N I     +T   R L    R+    P  T+ +     ++++ + +H +L+ +   + ++FP+   FE+  +        W    +  P+ +EE D DL    +  ++ Y    V+ICG+ ++ + LF ++      YFE+  EF  K   + ++  ++Q   +L +H   + +  L + +Y++ED   E YKE       +++       ++ E + ML  K+E +  + +  ++GP+ IE + +   E      D  +  Q I++N  +E SK+L  M    + +M  D+ ++    +
Sbjct:   37 VQWNVIECKFAVTCHNRTLQRQRRREEQAPADTQTSWAGLFSVSDLRNVHRQLTGVSDVLGAFFPDLSAFEVGNL--------WDLLFLGRPSGLEEPDMDLDWPCR-QLERYFSTAVDICGRTIVLDCLFAQDCRDVEEYFENLHEFKKKSMEDQVVRAREQLRKILQQHKAADKMVLLLK-IYEEED---EAYKELVTVATQLYQYLLQPFRDMRE-LAMLC-KME-IMKSLEFEDLGPKRIEALEKEAEEWRKRAQDAVSSIQDITVNYFVETSKALAGM----LKQMEEDRQRFGPAAW 319          
BLAST of SMED30008995 vs. TrEMBL
Match: A0A3B3Q5A6 (WASP homolog associated with actin, golgi membranes and microtubules OS=Paramormyrops kingsleyae OX=1676925 GN=WHAMM PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.705e-7
Identity = 77/324 (23.77%), Postives = 143/324 (44.14%), Query Frame = 3
Query:   18 LMFNDIYFNSYITFLTRILLDLSRKVSNFPITTKITSV---TIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGF-FSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENAT---YFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESI-----FEGFFIAHNNINEKVEMLIEKLEKLFANRKSAE---IGPRIIEEIN---QSIAEHLNDYYAH-QRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTEN 932
            + +N I     +T   R L    R   + P  T+ +     ++++ + +H + S +   +  +FP+  TFE   I        W   F  ++    E D DL +T    ++ YL A V++CG++++ + LF ++      YFE+ +EF  +   + I   K     +L +H   + L  L + +Y++ED   E Y+E +     F  + +       ++ ML  K+E L    KS E   +GP+ I ++    +   E   D  +  Q I++N  +E SK+L  M    + +M  D+ ++    +  C   RL  L  +   E 
Sbjct:   35 VQWNVIECKFAVTCHNRTLQRRRRTEESTPGDTQTSWAGLFSVSDLRNVHRQFSGVSDLLEPHFPDLSTFESGNI--------WDLIFPSRASGPEESDTDL-DTPCRQLERYLGAAVDVCGRKIVLDSLFVQDERDVEEYFENLQEFKRRTMQDQIDRAKGHLRTILHQHKTADKLVVLLK-IYEEED---EAYRELVTVATHFYQYLLQPFRDMRELAMLY-KMEIL----KSLEFDDLGPKRIADLEREAEGWRERAEDAVSSIQDITVNYFVETSKALAGM----LKQMEEDRRRFGQASW-SCAAPRLERLKFMLAKET 335          
BLAST of SMED30008995 vs. TrEMBL
Match: A0A2T7NE37 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_19929 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.541e-6
Identity = 61/275 (22.18%), Postives = 120/275 (43.64%), Query Frame = 3
Query:  111 TTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENAT-YFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAEHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTEN 932
            +++ T+ +I E KGI   LS + P + +YFP       F  E    R  W  FS  +P   E    + +    D++ +    +++C + LL   LF+E +   YFE   E   +   E + N +++  NL  + +Q   ++   E LY +ED++L    E++   +        +  EM ++K +      ++ ++G R+ EE  ++  E   ++     +  ++ +        +Y   +S+M  D+ ++    F      R+  L    H EN
Sbjct:   55 SSRSTAFSIQELKGIMQVLSLVHPTLANYFPH------FPDE---PRGIWAIFS--TPKTPENAAQICQ----DLEKFFFLALDVCKERLLMATLFEEPDTKEYFESMGELRKQGLLEEMRNAEEELKNLEFEREQCTAMKDYAE-LYVREDVSLNKLLEALAHFYNWELQPFLDLREMALKKAQNAKDMLQNPDVGNRVKEESKKAFTEWQAEHVRALDVIQDLYIVYYSRTSDLYRDLLSKMEEDRQRFGKNAFELVAAERVLRLKTQMHKEN 313          
BLAST of SMED30008995 vs. TrEMBL
Match: A0A3Q3X0L9 (WASP homolog associated with actin, golgi membranes and microtubules OS=Mola mola OX=94237 GN=WHAMM PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.721e-6
Identity = 73/300 (24.33%), Postives = 134/300 (44.67%), Query Frame = 3
Query:   18 LMFNDIYFNSYITFLTRILLDLSRK----VSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFF--SVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENAT---YFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALEN---YKESIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAE---IGPRIIEEINQSIAE---HLNDYYAH-QRISINMNLEMSKSLKSMYSSFISEMLNDKSKY 860
            + +N I     +T   R L    RK    V   P T+     ++++ K +H +LS +   + + FP+ + FE   I        W F   S +S T  + ++DL ET    ++ YL   +++CG++++ + LF +++     YFE+ +EF  K   E +   K +   LL  H   + + +L   + + ED A ++      + F+       ++ E     + K+E L    KS E   +GP+ I  + +   E      D  A  Q I++N   + SK+L  M    + +M  DK ++
Sbjct:   35 VQWNVIECKFAVTCHNRTLQRQRRKDEVAVGGDPQTSWAGLFSVSDLKHVHQQLSCVSDILGACFPDLVEFEEGNI--------WDFLFPSRRSGTRDDHERDL-ETPCRKLEKYLSTAIDVCGRKIVLDTLFVQDDRDVEEYFENLQEFRKKTMQEEMARAKARLRQLLQSHGGADGMVALL-CICEDEDEAYQDLVTVATAFFQYLLQPFRDMRELA--CLYKMEIL----KSLEFEDLGPKRIAALEKEAEEWRMKAEDAVASIQDITVNYFAQTSKALAGM----LRQMEEDKCRF 314          
BLAST of SMED30008995 vs. Planmine SMEST
Match: SMESG000022730.1 (SMESG000022730.1)

HSP 1 Score: 1088.17 bits (2813), Expect = 0.000e+0
Identity = 536/538 (99.63%), Postives = 537/538 (99.81%), Query Frame = 3
Query:   78 DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAEHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA 1691
            DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVE+LIEKLEKLFANRKSAEIGPRIIEEINQSI EHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA
Sbjct:   43 DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVEILIEKLEKLFANRKSAEIGPRIIEEINQSITEHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA 580          

HSP 2 Score: 124.02 bits (310), Expect = 3.655e-29
Identity = 89/98 (90.82%), Postives = 89/98 (90.82%), Query Frame = 2
Query: 1679 EESR*KN*KL**KEEPKQTVRKSSAKKMSSNHTPSMAKPPKEFSILASKCPSEPPSPYIVT--XXXXXXXXXXXXXXXXXVVTFVKIYNNKSSSSEVS 1966
            E  R  N KL  KEEPKQTVRKSSAKKMSSNHTPSMAKPPKEFSILASKCPSEPPSPYIVT  PPPPPPPAAAPPPPPPPVVTFVKIYNNKSSSSEVS
Sbjct:  581 ERIRNYNLKLKGKEEPKQTVRKSSAKKMSSNHTPSMAKPPKEFSILASKCPSEPPSPYIVTPPPPPPPPPAAAPPPPPPPVVTFVKIYNNKSSSSEVS 678          
BLAST of SMED30008995 vs. Planmine SMEST
Match: SMESG000022730.1 (SMESG000022730.1)

HSP 1 Score: 1086.25 bits (2808), Expect = 0.000e+0
Identity = 536/538 (99.63%), Postives = 537/538 (99.81%), Query Frame = 3
Query:   78 DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAEHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA 1691
            DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVE+LIEKLEKLFANRKSAEIGPRIIEEINQSI EHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA
Sbjct:   43 DLSRKVSNFPITTKITSVTIAEFKGIHYELSAIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSETSKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENILNCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHNNINEKVEILIEKLEKLFANRKSAEIGPRIIEEINQSITEHLNDYYAHQRISINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLYHTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSLIQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDDPINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEFQDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQYEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRIIESKKAA 580          
The following BLAST results are available for this feature:
BLAST of SMED30008995 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W5N5P23.327e-821.78WASP homolog associated with actin, golgi membrane... [more]
W5N5P93.401e-821.78WASP homolog associated with actin, golgi membrane... [more]
A0A3B3Q5A67.705e-723.77WASP homolog associated with actin, golgi membrane... [more]
A0A2T7NE371.541e-622.18Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A3Q3X0L96.721e-624.33WASP homolog associated with actin, golgi membrane... [more]
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BLAST of SMED30008995 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008995 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000022730.13.655e-2999.63SMESG000022730.1[more]
SMESG000022730.10.000e+099.63SMESG000022730.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008995 ID=SMED30008995|Name=SMED30008995|organism=Schmidtea mediterranea sexual|type=transcript|length=1968bp
GTTAAGCTTAACTTCATCTTATGTTTAATGATATTTATTTCAACAGTTAC
ATTACATTCCTTACACGAATATTGCTAGATCTTTCTCGAAAAGTTTCAAA
TTTCCCAATTACCACAAAAATAACATCAGTAACAATAGCTGAATTTAAGG
GTATTCATTATGAATTATCAGCTATAGAGCCTAAAATTTACTCGTATTTT
CCTGAAAATTTAACATTTGAAATTTTCACCATCGAAAGACCTAATACTAG
AAAATCGTGGGGATTTTTCAGCGTGCAATCTCCAACTGTTATAGAGGAAG
ACAAAGATTTATCTGAAACATCAAAACTTGACATTCAAAACTACCTTCGC
GCCGTAGTAGAAATCTGTGGTAAAGAATTATTGTTCAACGTTCTATTTAA
AGAAGAAAATGCTACATATTTTGAAGATAAAGAGGAATTTATTATTAAAA
AATCCAATGAAAATATACTTAATTGTAAACAACAGTGTATAAATTTGTTG
GGAAAACATGATCAATTTGAAAATCTTCAAAGTTTAACTGAAGATTTGTA
TAAACAAGAAGATTTAGCTTTAGAGAATTACAAAGAATCAATTTTTGAAG
GATTTTTTATTGCTCACAATAACATTAATGAAAAAGTAGAAATGTTGATT
GAAAAATTAGAAAAGTTATTTGCAAATCGTAAAAGTGCTGAGATAGGACC
AAGAATAATTGAAGAAATAAATCAATCTATTGCTGAGCATCTCAATGATT
ATTATGCTCATCAAAGAATTTCCATAAATATGAATCTTGAAATGTCAAAG
AGTTTGAAATCCATGTATTCTAGTTTTATTTCTGAAATGTTAAACGACAA
ATCCAAATATGAAACTATAATATTTAGAGATTGTATCGGTTCAAGATTAG
CAGATTTATCATTATTGTATCACACAGAAAATGAAAAAGTTTCTCATTAT
AATTTATTATTACATTCGACAAGTGAATTAATTTCCAAACGAAAGCTTCA
ATTAGTGTGTCATTGGGAAGAAGGAAAAGTAATGGTGAAACCTAATGTCC
GACACGAGGATTATTCATTAATACAATTAAAGAATTTAAATCCAACCAAA
ACCGACATTTCAGAAATCGAAAAGTTTAATGTCTATATAACTCATGCTTA
CAAACTGTTGTTGAGTTATTACGAAGCCAGACGGTCCAATTTATTAAATC
AAACACAAATTATAGATGATCCTATTAATGTCGTTCTTCCGGTAAATTTG
TCAGCTAAAACTCGTTCTATAAATGTAAATAGAAATGCAAACGATGAGCG
TAATTTATTTCAAACCATGAATATGCCAACTTTGTTGAATATTGATGAGG
AGGACTCCGACGAGGAATTCCAAGATGCTATCGATGATGTCAAATTCCAA
CATTTGAAAAATTTGGAAACTGAAAAAATGAAACTAAAAGAAAGTTTACA
GGATGAACTGAAATCTATTGCTAACATCAAAGCTGAATTAAGAAATTCTC
AACATCAATTGAAAGCCCAATATGAAAAATTGAAGAACAAAATGAATGAT
ACATTGGATAATGTCGGTGAATCTTTACAATTTATCTCTCTATATCCAGA
ATTTAATATTAAACCACCTAAAAAGTCACTTCCTGTTCCGGGATCAAATT
TAACTAAAGAACAGCGTATAATTGAATCGAAGAAAGCCGCTGAAAGAATT
AGAAACTATAATGAAAAGAAGAACCGAAACAAACTGTAAGAAAATCTTCT
GCAAAAAAAATGTCTTCTAATCATACACCTTCAATGGCTAAGCCACCTAA
AGAATTTTCAATTCTCGCATCAAAATGTCCGTCTGAACCTCCTTCTCCTT
ATATAGTAACACCTCCACCGCCTCCGCCTCCAGCAGCAGCACCACCTCCA
CCACCACCTCCTGTAGTCACATTCGTAAAAATTTATAATAATAAAAGTTC
CAGTAGTGAAGTTTCAAC
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protein sequence of SMED30008995-orf-1

>SMED30008995-orf-1 ID=SMED30008995-orf-1|Name=SMED30008995-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=573bp
MFNDIYFNSYITFLTRILLDLSRKVSNFPITTKITSVTIAEFKGIHYELS
AIEPKIYSYFPENLTFEIFTIERPNTRKSWGFFSVQSPTVIEEDKDLSET
SKLDIQNYLRAVVEICGKELLFNVLFKEENATYFEDKEEFIIKKSNENIL
NCKQQCINLLGKHDQFENLQSLTEDLYKQEDLALENYKESIFEGFFIAHN
NINEKVEMLIEKLEKLFANRKSAEIGPRIIEEINQSIAEHLNDYYAHQRI
SINMNLEMSKSLKSMYSSFISEMLNDKSKYETIIFRDCIGSRLADLSLLY
HTENEKVSHYNLLLHSTSELISKRKLQLVCHWEEGKVMVKPNVRHEDYSL
IQLKNLNPTKTDISEIEKFNVYITHAYKLLLSYYEARRSNLLNQTQIIDD
PINVVLPVNLSAKTRSINVNRNANDERNLFQTMNMPTLLNIDEEDSDEEF
QDAIDDVKFQHLKNLETEKMKLKESLQDELKSIANIKAELRNSQHQLKAQ
YEKLKNKMNDTLDNVGESLQFISLYPEFNIKPPKKSLPVPGSNLTKEQRI
IESKKAAERIRNYNEKKNRNKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR031738JMY/WHAMM
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..219
NoneNo IPR availableCOILSCoilCoilcoord: 462..514
IPR031738JMY/WHAMM, middle domainPFAMPF15871JMYcoord: 37..306
e-value: 6.1E-19
score: 68.5