Meckelin
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008957 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Meckelin vs. Ensembl Human
Match: TMEM67 (transmembrane protein 67 [Source:HGNC Symbol;Acc:HGNC:28396]) HSP 1 Score: 481.871 bits (1239), Expect = 1.174e-153 Identity = 332/983 (33.77%), Postives = 494/983 (50.25%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTACATN---YRPVN---NQCQLCSNILSTNGNSSCSCALP---TGNACLTKS----LSNSSILTY--LGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV--QPSGSYTSGKTAGY----PWLYY-----TIPQTLTQLTVDHQY-VRGET----LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLEDGRNM------QYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C + YF CV C N +Q + G SCVC G+ N GG ++C+KCP N + + +GW+CISC + +T C ++ D+ G + C C N + VN ++C C N + SC+C+ P TG C + + L S Y +G S+TS + L S A C V + CQ L NMCVM++ N + D C L+ + +G T + + PWL+Y PQ L+ ++ + +GE L+ V A+YD G FL + + LQLC ++ T+ + FGT Y ++C I IS++ P+FYD YL YTD + +P+ + + + N + W+LTRR+ LVD T + S+ + +V T +G+IY P + + + + S + +SF V Y + +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF LK L+ +H ++Q T+D+F IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ VL F+EV+GFKN+ D + S + +Y AP + R+AV +++ IQ +F + YER IEDK+R +VDLCS++NIS+ +L+H+ FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ N R + D ++ + G RLL + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL QN + +F+T+ I + IRN G NLA+KTLVD+RFLI
Sbjct: 39 CDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTF-VNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNM-NSYDFATFDA--CGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQ-TLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFP-TPIFYDVYLEYTDENQHQYILAVPV-LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPN-------TINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEA---HVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI 985
BLAST of Meckelin vs. Ensembl Human
Match: TMEM67 (transmembrane protein 67 [Source:HGNC Symbol;Acc:HGNC:28396]) HSP 1 Score: 481.1 bits (1237), Expect = 2.794e-153 Identity = 332/983 (33.77%), Postives = 494/983 (50.25%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTACATN---YRPVN---NQCQLCSNILSTNGNSSCSCALP---TGNACLTKS----LSNSSILTY--LGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV--QPSGSYTSGKTAGY----PWLYY-----TIPQTLTQLTVDHQY-VRGET----LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLEDGRNM------QYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C + YF CV C N +Q + G SCVC G+ N GG ++C+KCP N + + +GW+CISC + +T C ++ D+ G + C C N + VN ++C C N + SC+C+ P TG C + + L S Y +G S+TS + L S A C V + CQ L NMCVM++ N + D C L+ + +G T + + PWL+Y PQ L+ ++ + +GE L+ V A+YD G FL + + LQLC ++ T+ + FGT Y ++C I IS++ P+FYD YL YTD + +P+ + + + N + W+LTRR+ LVD T + S+ + +V T +G+IY P + + + + S + +SF V Y + +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF LK L+ +H ++Q T+D+F IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ VL F+EV+GFKN+ D + S + +Y AP + R+AV +++ IQ +F + YER IEDK+R +VDLCS++NIS+ +L+H+ FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ N R + D ++ + G RLL + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL QN + +F+T+ I + IRN G NLA+KTLVD+RFLI
Sbjct: 49 CDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTF-VNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNM-NSYDFATFDA--CGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQ-TLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFP-TPIFYDVYLEYTDENQHQYILAVPV-LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPN-------TINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEA---HVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI 995
BLAST of Meckelin vs. Ensembl Human
Match: TMEM67 (transmembrane protein 67 [Source:HGNC Symbol;Acc:HGNC:28396]) HSP 1 Score: 418.698 bits (1075), Expect = 1.626e-130 Identity = 295/866 (34.06%), Postives = 440/866 (50.81%), Query Frame = 3
Query: 504 VNNQCQLCSNILSTNGNSSCSCALP---TGNACLTKS----LSNSSILTY--LGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV--QPSGSYTSGKTAGY----PWLYY-----TIPQTLTQLTVDHQY-VRGE----TLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLEDGRNM------QYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+ ++C C N + SC+C+ P TG C + + L S Y +G S+TS + L S A C V + CQ L NMCVM++ N + D C L+ + +G T + + PWL+Y PQ L+ ++ + +GE L+ V A+YD G FL + + LQLC ++ T+ + FGT Y ++C I IS++ P+FYD YL YTD + +P+ + + + N + W+LTRR+ LVD T + S+ + +V T +G+IY P + + + + S + +SF V Y + +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF LK L+ +H ++Q T+D+F IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ VL F+EV+GFKN+ D + S + +Y AP + R+AV +++ IQ +F + YER IEDK+R +VDLCS++NIS+ +L+H+ FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ N R + D ++ + G RLL + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL QN + +F+T+ I + IRN G NLA+KTLVD+RFLI
Sbjct: 85 LGDRCVRCEPTF-VNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNM-NSYDFATFDA--CGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQ-TLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFP-TPIFYDVYLEYTDENQHQYILAVPV-LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPN-------TINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEA---HVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI 914
BLAST of Meckelin vs. Ensembl Human
Match: TMEM67 (transmembrane protein 67 [Source:HGNC Symbol;Acc:HGNC:28396]) HSP 1 Score: 105.916 bits (263), Expect = 1.406e-23 Identity = 108/397 (27.20%), Postives = 173/397 (43.58%), Query Frame = 3
Query: 351 SSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTACATN---YRPVN---NQCQLCSNILSTNGNSSCSCALP---TGNACLTKS----LSNSSILTY--LGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV--QPSGSYTSGKTAGY----PWLYY-----TIPQTLTQLTVDHQY-VRGET----LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEV 1442
+ +GW+CISC + +T C ++ D+ G + C C N + VN ++C C N + SC+C+ P TG C + + L S Y +G S+TS + L S A C V + CQ L NMCVM++ N + D C L+ + +G T + + PWL+Y PQ L+ ++ + +GE L+ V A+YD G FL + + LQLC ++ T+ + FGT Y ++C I IS++ P+FYD YL YTD + +P+ + + + N + W+LTRR+ LVD T + S+ + +V T +G+IY P + +
Sbjct: 5 TEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTF-VNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNM-NSYDFATFDA--CGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQ-TLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFP-TPIFYDVYLEYTDENQHQYILAVPV-LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPN-------TINGNIYPPLITI 387
BLAST of Meckelin vs. Ensembl Human
Match: TMEM67 (transmembrane protein 67 [Source:HGNC Symbol;Acc:HGNC:28396]) HSP 1 Score: 65.4698 bits (158), Expect = 1.706e-11 Identity = 32/94 (34.04%), Postives = 48/94 (51.06%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLG 455
C + YF CV C N +Q + G SCVC G+ N GG ++C+KCP N + + +GW+CISC + +T C ++ D+ G
Sbjct: 47 CDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDING 140
BLAST of Meckelin vs. Ensembl Celegans
Match: mks-3 (MecKel-Gruber Syndrome (MKS) homolog [Source:UniProtKB/TrEMBL;Acc:Q20046]) HSP 1 Score: 318.546 bits (815), Expect = 7.359e-94 Identity = 270/967 (27.92%), Postives = 437/967 (45.19%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVC-KDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFNGN--CTACATN-YRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGYPW------LYYTIPQTL-TQLTVDHQYV--RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLL-FDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGI-SGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQN--LFSILPPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAP---NYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPG--------GNRLLEDGRNM---QYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDN-GTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C+ YF+ ++ +C C S D SC C K+ +NG C++CP + S + C++C NG +C C S + D GN + C CA+ YR N C C +SCS T + + + + G + S +++ +L +C C+ + NMCV+ N +S S++ C L + K PW L T L + +DHQY+ + ++ A YD G F G ++D L CD+S+ F FG Y + C I + ++ LF + YL ++D G S+LYP+ I + + +G N D+ W+LTRR L D++ + + ++ + FA+ + + VV Q +DG I PYL + + + S E++Y+ SS + L +A+ I+ ++ ++ L ++ W RR G S + +L + + +VFF++ I+ + +K+Q L+++L D + F Y I++ +LK ++ A + F IDWER K + TD G K ++ V +WRTY IANEWNE+Q YRKTSL +I ++L ME KN V DA+ + + AV + ++ A +Q L VLI ER++ D N+VDLCS++NIS++ L H +GYYIHGRSVHG D +SEM LQ+E C RGLE S QT+ + LPN FR D ++ G+ + N + ++N FL +F++ + I + +DR E + D E D+S+ G F RD ++ YG E A +F+ L V L + F+ + + ++++I ++L +LVD+RFL+
Sbjct: 5 CTRLQYFNRIELRCEICPNTTIPSDDMMSCKCSKNQIEIFRNGFVPT--CEECPEGTLSSPDQTGCLNCNNG----TCH-CPSQSTLIFRDASGNLLTNDAFCGNCASGFYRNDNGYCTKCE--------TSCSEMNSTNSDQVYTRIKLQN-----GVEMKSIYIENHLSTDAKLCSERNIQSCEAMVNMCVLQ--NFESGSSLNA--CGLLENI---------KRLFNPWQNTLDALATTDDSELENENVIDHQYLFRPDSSFELFFARYDVNGTFDGFF-TSEDIFLHFCDDSLNPFA-PFLFGRRYQKECKIRKATFTDPRLERKLF-EVYLKFSDEQGHSKLYPVHI-LNSAVRVNGQLLNFANKDRNRWVLTRRFYLFDDYS-----MKFDNSSKLMRFAAKIGINVVLQKE------RDGYINPPYLTIEYDDSTDEIFEN----SLEIIYHKDSS---GYDQKLIIALSIIIPMSLFWSALCSYSWGRRQGKPSAVDASSILYFFVCEVSILGDVFFVLFGFIACWTTFAYKSQTYVLYNMLSYDQEKFLFHYFIASLVLKFFGLLFTMGALVFQETFFIDWERQKLR--------------QTDEHGMPLSRDLHKSTEAEPVVVWRTYLIANEWNELQQYRKTSLALQVITMILLMEYFQVKNYA----LVEPEFERDAFDSSTTLSTLASSLAVTIFVYLSLALLQVLVRVLIVERIVTDPFHNFVDLCSLSNISVLSLTHSLYGYYIHGRSVHGKGDAGMSEMNEFLQRERNNLCGFRGLEPGSELQTFTVNLPNLFRSKYDEIFAVSKQTTSGVVGHEAITAKMNATVDAHSQMNSFLKKFVDHSI-TDIEYVVRDRPVLESVLDMEMSDSSITGTFTRDPVEIAYSRCFVYGNEWAWTSFECLTVTVFYLWSGSLYLAGFVVYVISHLIRLIFGYLSTNHLIKTSLVDQRFLV 897
BLAST of Meckelin vs. Ensembl Fly
Match: CG15923 (gene:FBgn0038814 transcript:FBtr0113254) HSP 1 Score: 132.494 bits (332), Expect = 4.939e-31 Identity = 109/413 (26.39%), Postives = 188/413 (45.52%), Query Frame = 3
Query: 1809 LISAFLLKMLEI-VHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLE----------------DGR----NMQYLEVNKFLDRFINRT-HDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
L +AF+L+ L + +H++ + +++FLIDWERP+ +++ D+++ + + + + + VS WR F AN W + +K S + + +V + G++ + F D + R +I + + +T +Q ++ ++ I + L+ ++DLCS+ANIS+ L FGYYIHGRS HG AD +S M LQ+ C +RGL DS +Q Y++ P N I L+ + P L DG+ ++ Y VN+F FI+ D + + + + + G FY D+ SF VL G L + ++LL L+ N + A L +L+ + N++ KTL+D RFL+
Sbjct: 590 LNTAFVLQFLFLGIHLWRSS-QLELFLIDWERPRSSCEGQRL----NLDSSSLCSSVRTFVAE------SSVSAWRVLFTANAWIRLSVTQKYSTLGQVFVV-----IAGYQFLERFT-------KGDIF-------LRCCLIAAAYLLTYLLQ----IIGHQLFIANPLQKFIDLCSLANISLFSLTEPGFGYYIHGRSPHGFADTDMSSMILQLQRTQT-MCGRRGLLMDSDKQAYVILPPRNLHIYLERLLLPFQRSLTGTLSQTMLYQKDIVPSIDGQMERTSIAYASVNRFFCAFIDHAIKDMDYIIKEKSFVEQLLNCEIDNYITENRGTFYIDDSFSFSRVLLLGNHLHIFVLELLLVLSIFLMTSNLLIAAAGACALNILLRQVFRHWVRRNISRKTLIDERFLL 967
BLAST of Meckelin vs. Ensembl Fly
Match: CG15923 (gene:FBgn0038814 transcript:FBtr0330028) HSP 1 Score: 132.109 bits (331), Expect = 5.661e-31 Identity = 109/413 (26.39%), Postives = 188/413 (45.52%), Query Frame = 3
Query: 1809 LISAFLLKMLEI-VHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLE----------------DGR----NMQYLEVNKFLDRFINRT-HDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
L +AF+L+ L + +H++ + +++FLIDWERP+ +++ D+++ + + + + + VS WR F AN W + +K S + + +V + G++ + F D + R +I + + +T +Q ++ ++ I + L+ ++DLCS+ANIS+ L FGYYIHGRS HG AD +S M LQ+ C +RGL DS +Q Y++ P N I L+ + P L DG+ ++ Y VN+F FI+ D + + + + + G FY D+ SF VL G L + ++LL L+ N + A L +L+ + N++ KTL+D RFL+
Sbjct: 590 LNTAFVLQFLFLGIHLWRSS-QLELFLIDWERPRSSCEGQRL----NLDSSSLCSSVRTFVAE------SSVSAWRVLFTANAWIRLSVTQKYSTLGQVFVV-----IAGYQFLERFT-------KGDIF-------LRCCLIAAAYLLTYLLQ----IIGHQLFIANPLQKFIDLCSLANISLFSLTEPGFGYYIHGRSPHGFADTDMSSMILQLQRTQT-MCGRRGLLMDSDKQAYVILPPRNLHIYLERLLLPFQRSLTGTLSQTMLYQKDIVPSIDGQMERTSIAYASVNRFFCAFIDHAIKDMDYIIKEKSFVEQLLNCEIDNYITENRGTFYIDDSFSFSRVLLLGNHLHIFVLELLLVLSIFLMTSNLLIAAAGACALNILLRQVFRHWVRRNISRKTLIDERFLL 967
BLAST of Meckelin vs. Ensembl Zebrafish
Match: tmem67 (transmembrane protein 67 [Source:ZFIN;Acc:ZDB-GENE-080716-1]) HSP 1 Score: 470.7 bits (1210), Expect = 1.029e-149 Identity = 324/986 (32.86%), Postives = 490/986 (49.70%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCP-LNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACATNYRPVN------NQCQLCSNILSTNGNSSCSCALP---TGNAC-----LTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV------QPSGSYTSGKTAGYPWLYYTIPQTLTQLTVDHQYV----------RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYL-PLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDI---TKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL-P-PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRP-----GGNRLLEDGRN------MQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C +YF CV C N S G +C C+ G + G S +LCQ+CP +Q+ + +G+ CI C V C +V D GN C AC+ + N+C+ C + N + SC C G C L S+++S I + L V S + + + S A C + S + CQ+L NMCVM N S+ SI+ C LY + Q S S PWLYY + + ++ + + +++ A Y G+FL + + NLQLC ++ T+++ F+FGT Y +SC +++S+L T Y PLFYD +L +S ++L +P+ + +G N D + W LTRRL+LVD ++ I AS +++ F + T+ G +Y P + V T + ++F V Y + +A+G+L +A ++++L+ W RR + ++K L ++NVFFI+ G LY+L+ FK Q S+L P P + FV Y+ AF LK ++ +H Q +VD+F IDWERP R K + +G E SQ + VSIWRTYF+ANEWNEIQT RK + F ++ VL F+EV+GF N+ D N + S++ Y+ P + R+ V +++ IQ +F + +ER +EDK+R +VDLCS++NIS+++L+HR FGYYIHGRSVHG AD ++ EM L++E C +RGL +S QT+ +++ N R D + P G +RL++ N Y +N+FL I+ H + + +D+ FE + EF + + FY D+ SF +VLFYG E L+ FD L FCV DL QN + A +T+ I ++IRNG G NLANKTLVD+RFLI
Sbjct: 39 CGVNSYFDISSLSCVKCGSNQVSSKSGLACECQAGSRVLVSNGVS-ILCQQCPDADQVVTQDGYECIRCPGTVGKDGKCQCPEGSVLVERDGNGNPLIEARCQACSATDPAFSAPDMKGNRCERCGDSF-INSSQSCVCGTSNTLAGGMCFPANSLPTSVASSVIFSQLTLPVQSAWFSSFMYSSAAACLLFSNRTACQSLGNMCVM---NMHSSSSINNDVCGLYNTIYRTTAAQGSSQDISYWRTNLPWLYYGDQPGMASQALQNEPLPVGFSFKGAQKNTNIELRAAVYSVRGEFLRWESVGR-GNLQLCPDTPTRQQSAFKFGTAYKQSCVLSVSEL--LTGYPEPLFYDVFLVIQNS-QDNRLLAVPL-LNANQQFNGQFVNQGSDMSKWFLTRRLMLVDTLSGREKSISSSPVV--IRVASDIKI--------GFQLVPNTQKGQVYPPLMSVAYSDIQIKDPSTQTVTVTFSVEY---TMNQEEARIKTDIALGVLGGVAVVHSLLKTASWKRRIASPLIDLETIMKFLVFYAGDLANVFFIITVGTGLYWLIFFKAQLFVSVLLPLPAQEERFVVYVGCAFALKTVQFLHKIFVQLSVDVFFIDWERP-----------RGKTTKHVEGSG-------ETKSQASPVSIWRTYFVANEWNEIQTIRKINPTFQVMAVLFFLEVVGFSNLALRDPSSNLNRSAEEYTPPYSLILRYGVATAMWLCIGLIQMIFFTVFHERFVEDKIRQFVDLCSISNISVLLLSHRCFGYYIHGRSVHGHADTNMDEMNTNLKREAENLCGQRGLLPNSDTQTFQISITNRLRAQYDRIIEPISRRRGPSRLVDATANPCEQSTKAYHTMNRFLGSVIDHAHR-EMDYIVKDKLLFERVIGMEFIEPLDKSIFYNDDSHSFADVLFYGNEGVLLIFDTLFFCVVDLGKQNFILAAVLTYLQQMIFRLIRNGLGRRNLANKTLVDKRFLI 982
BLAST of Meckelin vs. Ensembl Xenopus
Match: sfxn5 (sideroflexin 5 [Source:Xenbase;Acc:XB-GENE-988100]) HSP 1 Score: 454.521 bits (1168), Expect = 2.478e-143 Identity = 326/998 (32.67%), Postives = 493/998 (49.40%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACLTK--SLSNSSILTY----LGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAG-----------YPWLYYTIPQTLTQLTV------------DHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPI-----KIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFG----AITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLE------DGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C Y+ + CV C + + S G SCVC GY +N G C++CP + +++GWSCI C NG+ C N+ DV G+ C C T + N+C+ C + + N ++SCSC P TG C S+ + ++LT LG + S + +L S A C + + ++ CQ L NMCVM++++ S S D C L+ + +TS G PWL+Y L T+ + + V LQ + A YD G F+ + + LQLC + + + F FGT Y + C I++S+L + P+FYD YL+Y + L P+P+ + ++ + G N W+LTRR+ LVD ++ P + R AS IT S+RLV T G IY P + V + + +SF V N VA+G+L LA L+++L+ W RR G S + ++ L ++++VFF+V G Y+L+LFK Q S+L P+++ FV+Y+ AF LK L++++ T+D+F IDWERPK + P + ++K A VSIWR+YFIANEWNEIQT RK S +F + VL F+E+ GFK + D + S +S Y AP R+ R+ V +I+ +Q LF +IYER +EDK+R +VDLCS++NIS+ +L+HR +GYYIHGRSVHG +D ++ EM L++E C++RGL+ +S QT+ +++ + R + D ++ R G +RL D Y +NKFL FI+ + + +D+ F E + EF + + FY D SF +VLFYG E L+ FD L FCV D+ QN V A +T+ + +++ N G NLA+KT +D RFLI
Sbjct: 50 CGLQEYYEPSNLSCVRCQNSQRPSHWGTSCVCLVGY--KRNSTGD---CERCPESLGVTADGWSCIKCPNGLNSNGNCLCPPGNILVERDVNGSLLLEAECIDCDGTEPSFTAANTLGNRCERCHHTV-INRSNSCSCNQPNIMTGGICYNAAGSILSKALLTVHYSQLGLILRSEWFATHLQASAAACLMYTNQTSCQALGNMCVMNMHS-TSISSADA--CGLFQYI-----FTSTAGLGVIHSLSYWRTNLPWLFYGDQPGLASRTLVSSPVPTSYTFKESEEVENTKLQFLAAVYDVRGNFIKWQ-SLAGGLLQLCPDIVARIDAAFSFGTTYKQECRISVSKLL-RDHPEPVFYDVYLAYPGNEKQQNLLPVPVLNLNLQYHEMFVNQGDNIN------NWLLTRRIFLVDTISGRENSLADPPRLIRVASKITI--SIRLV---------PDTYKGIIYPPLMTVQYTDVQLQNPESQIVKVSFSV---NYEMRQLDSLKKTDVAIGVLGGLAVLWSLLKTAAWKRRIGSSFIDMQTVIHFLIFYAGALADVFFLVTVGTGFYWLILFKGQQYVSVLLPVPEEEYNFVSYVWCAFALKALQLLYKMTLLLTIDVFFIDWERPKARTQPSE--GKTKNSVAP-------------------VSIWRSYFIANEWNEIQTVRKISPLFQVFAVLFFLEIAGFKYLALMDPSSSLSRNSQDYMAPWSRILRYGVSTAIWLAIGCLQVLFFSVIYERYVEDKIRQFVDLCSMSNISVFILSHRCYGYYIHGRSVHGHSDTNMEEMNLNLKREAENLCSQRGLQPNSDIQTFQISISSRARTHFDQIHESLTKRHGPSRLFHTSLSTSDLNTRAYNSMNKFLSSFIDHAFK-EVDYFVKDKMFLERLLSMEFMEPMDKSIFYNDENHSFTDVLFYGNESTLLIFDTLFFCVVDVASQNFVLAAILTFLQQELFRLLCNAVGKKNLASKTQIDERFLI 989
BLAST of Meckelin vs. Ensembl Xenopus
Match: sfxn5 (sideroflexin 5 [Source:Xenbase;Acc:XB-GENE-988100]) HSP 1 Score: 440.269 bits (1131), Expect = 2.592e-138 Identity = 323/983 (32.86%), Postives = 479/983 (48.73%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGY---------PWLYY-----TIPQTLTQLTVDHQYVRGET---LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPI-----KIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFG----AITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVI-GFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLE------DGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C Y+ + CV C + + S G SCVC GY +N G C++CP + +++GWSCI C NG+ C N+ DV G+ C C T + N+C+ C + + N ++SCSC P N + KS ++ Y Q+ CQ L NMCVM++++ S S D C L+ + S T+G + Y PWL+Y +TL V Y E LQ + A YD G F+ + + LQLC + + + F FGT Y + C I++S+L + P+FYD YL+Y + L P+P+ + ++ + N W+LTRR+ LVD ++ P + R AS IT S+RLV T G IY P + V + + +SF V N VA+G+L LA L+++L+ W RR G S + ++ L ++++VFF+V G Y+L+LFK Q S+L P+++ FV+Y+ AF LK L++++ T+D+F IDWERPK + P S+ D A VSIWR+YFIANEWNEIQT RK S +F + VL F+EVI GFK + D + S +S Y AP R+ R+ V +I+ +Q LF +IYER +EDK+R +VDLCS++NIS+ +L+HR +GYYIHGRSVHG +D ++ EM L++E C++RGL+ +S QT+ +++ + R + D ++ R G +RL D Y +NKFL FI+ + + +D+ F E + EF + + FY D SF +VLFYG E L+ FD L FCV D+ QN V A +T+ + +++ N G NLA+KT +D RFLI
Sbjct: 50 CGLQEYYEPSNLSCVRCQNSQRPSHWGTSCVCLVGY--KRNSTGD---CERCPESLGVTADGWSCIKCPNGLNSNGNCLCPPGNILVERDVNGSLLLEAECIDCDGTEPSFTAANTLGNRCERCHHTV-INRSNSCSCNQP--NIMVRKSFR---VIMYTNQT-----------------------SCQALGNMCVMNMHS-TSISSADA--CGLFQYIFTS---TAGLGSKYHVFYRRTNLPWLFYGDQPGLASRTLVSSPVPTSYTFKEKNTKLQFLAAVYDVRGNFIKWQ-SLAGGLLQLCPDIVARIDAAFSFGTTYKQECRISVSKLL-RDHPEPVFYDVYLAYPGNEKQQNLLPVPVLNLNLQYHEMFVNQDNINN-------WLLTRRIFLVDTISGRENSLADPPRLIRVASKITI--SIRLV---------PDTYKGIIYPPLMTVQYTDVQLQNPESQIVKVSFSV---NYEMRQLDSLKKTDVAIGVLGGLAVLWSLLKTAAWKRRIGSSFIDMQTVIHFLIFYAGALADVFFLVTVGTGFYWLILFKGQQYVSVLLPVPEEEYNFVSYVWCAFALKALQLLYKMTLLLTIDVFFIDWERPKARTQP------SEGDVAP-------------------VSIWRSYFIANEWNEIQTVRKISPLFQVFAVLFFLEVIAGFKYLALMDPSSSLSRNSQDYMAPWSRILRYGVSTAIWLAIGCLQVLFFSVIYERYVEDKIRQFVDLCSMSNISVFILSHRCYGYYIHGRSVHGHSDTNMEEMNLNLKREAENLCSQRGLQPNSDIQTFQISISSRARTHFDQIHESLTKRHGPSRLFHTSLSTSDLNTRAYNSMNKFLSSFIDHAFK-EVDYFVKDKMFLERLLSMEFMEPMDKSIFYNDENHSFTDVLFYGNESTLLIFDTLFFCVVDVASQNFVLAAILTFLQQELFRLLCNAVGKKNLASKTQIDERFLI 943
BLAST of Meckelin vs. Ensembl Xenopus
Match: sfxn5 (sideroflexin 5 [Source:Xenbase;Acc:XB-GENE-988100]) HSP 1 Score: 366.696 bits (940), Expect = 1.571e-111 Identity = 290/903 (32.12%), Postives = 435/903 (48.17%), Query Frame = 3
Query: 414 CDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGS------YTSGKTAGYPWLYY-----TIPQTLTQLTVDHQYVRGET---LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETF--RFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPI-----KIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFG----AITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXX-XXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVI-GFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLE------DGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C + DV G+ C C T + N+C+ C + + N ++SCSC P N + KS ++ Y Q+ CQ L NMCVM++++ S S D C L+ + S + S PWL+Y +TL V Y E LQ + A YD G F+ + + LQ+ + + T+ + G C I++S+L + P+FYD YL+Y + L P+P+ + ++ + G N W+LTRR+ LVD ++ P + R AS IT S+RLV T G IY P + V T + + +SF V N VA+G+L LA L+++L+ W RR G S + ++ L ++++VFF+V G Y+L+LFK Q S+L P+++ FV+Y+ AF LK L++++ T+D+F IDWERPK R+ F + T + VSIWR+YFIANEWNEIQT RK S +F + VL F+EVI GFK + D + S +S Y AP R+ R+ V +I+ +Q LF +IYER +EDK+R +VDLCS++NIS+ +L+HR +GYYIHGRSVHG +D ++ EM L++E C++RGL+ +S QT+ +++ + R + D ++ R G +RL D Y +NKFL FI+ + + +D+ F E + EF + + FY D SF +VLFYG E L+ FD L FCV D+ QN V A +T+ + +++ N G NLA+KT +D RFLI
Sbjct: 35 CTCHKITVERDVNGSLLLEAECIDCDGTEPSFTAANTLGNRCERCHHTV-INRSNSCSCNQP--NIMVRKSFR---VIMYTNQT-----------------------SCQALGNMCVMNMHS-TSISSADA--CGLFQYIFTSTAGLGVIHSLSYWRTNLPWLFYGDQPGLASRTLVSSPVPTSYTFKEKNTKLQFLAAVYDVRGNFIKWQ-SLAGGLLQIHNYTYTQSIFCYPCSLGHIIPLQCRISVSKLL-RDHPEPVFYDVYLAYPGNEKQQNLLPVPVLNLNLQYHEMFVNQGDNIN------NWLLTRRIFLVDTISGRENSLADPPRLIRVASKITI--SIRLV---------PDTYKGIIYPPLMTVQYTDVQLQNPESQIVKVSFSV---NYEMRQLDSLKKTDVAIGVLGGLAVLWSLLKTAAWKRRIGSSFIDMQTVIHFLIFYAGALADVFFLVTVGTGFYWLILFKGQQYVSVLLPVPEEEYNFVSYVWCAFALKALQLLYKMTLLLTIDVFFIDWERPK----------RTFFSFLSGKTKNSV----------APVSIWRSYFIANEWNEIQTVRKISPLFQVFAVLFFLEVIAGFKYLALMDPSSSLSRNSQDYMAPWSRILRYGVSTAIWLAIGCLQVLFFSVIYERYVEDKIRQFVDLCSMSNISVFILSHRCYGYYIHGRSVHGHSDTNMEEMNLNLKREAENLCSQRGLQPNSDIQTFQISISSRARTHFDQIHESLTKRHGPSRLFHTSLSTSDLNTRAYNSMNKFLSSFIDHAFK-EVDYFVKDKMFLERLLSMEFMEPMDKSIFYNDENHSFTDVLFYGNESTLLIFDTLFFCVVDVASQNFVLAAILTFLQQELFRLLCNAVGKKNLASKTQIDERFLI 862
BLAST of Meckelin vs. Ensembl Xenopus
Match: ENSXETT00000046533.1 (meckelin-like [Source:NCBI gene;Acc:100485117]) HSP 1 Score: 209.149 bits (531), Expect = 1.371e-59 Identity = 138/335 (41.19%), Postives = 195/335 (58.21%), Query Frame = 3
Query: 2010 NNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRP------GGNRLLEDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+ +SIWRT +ANEWNE+QTYRK S IF L VLL +EV+G KNIT D ++ +P S Y A + RF + S++ +Q LF ++IYER EDK+R + DLCS++N+S+ +L HR +GYYIHGRSVHG ADVS+ M L+KE C RGLE S QT+ + L + R + + P G + E MQ Y +N+FL F++ + + + +D+ F E IFD EF+ + Y D+G F LFYG EL L+ FD LLFC+ DL QN V IT+ + T++ ++R G N++ +T+V+ FLI
Sbjct: 7 KSNISIWRTILVANEWNEMQTYRKISPIFQLFSVLLLLEVVGLKNITAKDLNLDLNPPSGTYLASWSIILRFGIAASMWLAVGIVQVLFCIVIYERFFEDKMRQFTDLCSLSNVSVFILTHRCYGYYIHGRSVHGQADVSMETMLSNLKKEEENLCPLRGLEPSSDIQTFEVLLSDRVRDQYEKIMEPLLEAPRGQRGVNESNPLMQQRIKTYYTINRFLSSFLDHVYK-ELDYVVKDKLFLEHIFDMEFQQPMEKSIVYTDDGARFSRTLFYGNELVLLLFDTLLFCIIDLGTQNFVLATIITYVVQTLVVILRYQIGKKNVSRQTMVEENFLI 340
BLAST of Meckelin vs. Ensembl Mouse
Match: Tmem67 (transmembrane protein 67 [Source:MGI Symbol;Acc:MGI:1923928]) HSP 1 Score: 494.197 bits (1271), Expect = 1.842e-158 Identity = 338/988 (34.21%), Postives = 500/988 (50.61%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACLT------KSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV-QPSGSYTSGKTAGY-----PWLYY-----TIPQTL--TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPF----QTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYY----NVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLL-EDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C YF C C N ++ + G SCVC GY+ N GG ++C+KCP N + + +GW CISC +G+T C + ++ +V G+ C C T + +C C N + SCSC+ P TG C + + + +++ LG S+ S + L + A C + CQ L NMCVM++ N + ++D C L+ + + + S + + PWL+Y PQ L T L + + L+ V A+YD G F+ + + LQLC ++ + + FGT Y ++C I++S+L P+FYD YL YTD L+PIP+ + + + N + W+LTRR+ LVD + R A+ I+ S+RLV TK+G+IY P L + ++ + SA ISF V Y + S +T +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF +K L+ +H F++Q ++DIF IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ L F+EV+GFKN+ D + S + Y+AP R+ R+AV +I+ + IQ +F YER IEDK+R +VDLCS++N+S+ +L+HR FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ + R + D ++ R G RLL G + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL Q+ V +F+T+ I + IRN G NLA KTLVD RFLI
Sbjct: 52 CDFNQYFDISALSCAPCGANQRRDALGTSCVCLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPSGLTAEGKCHCPTGHILVERNVSGSLLAQATCELCDESENSFTKANALGTRCVRCEPTF-VNTSRSCSCSEPHTLTGGLCFSNTGNFHQRVISTARYGELGMSLNSEWFAKYLQATAAACWTHANLTSCQALGNMCVMNM-NSYDSTTLDA--CRLFHYIFESTAGLISVHSVPFWRQNLPWLFYGDQPGLAPQVLSTTPLPTNFSFKGQNQLKFVAASYDIRGNFIKWQ-PLEGGVLQLCPDTERRLDAAYAFGTTYQQNCEISLSKLLVDFSS-PVFYDVYLEYTDEEQHRYLWPIPV-LNLNLQHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISL--SIRLV---------PNTKNGNIYTPLLTIAYSDIDIKNAHSQSAKISFSVKYEMNQGDASVHT-------DIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFISQISIDIFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQVVFFAAFYERFIEDKIRQFVDLCSMSNVSVFLLSHRCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFQIAVSSQMRQHYDRIHETLTRRNGPARLLSSSGSTFEQSIKAYHAMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGHSFSSVLYYGNEATLLIFDLLFFCVVDLACQDFVLASFLTYLQQEIFRFIRNTVGQKNLATKTLVDERFLI 995
BLAST of Meckelin vs. Ensembl Mouse
Match: Tmem67 (transmembrane protein 67 [Source:MGI Symbol;Acc:MGI:1923928]) HSP 1 Score: 494.197 bits (1271), Expect = 1.064e-157 Identity = 338/988 (34.21%), Postives = 500/988 (50.61%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACLT------KSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV-QPSGSYTSGKTAGY-----PWLYY-----TIPQTL--TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPF----QTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYY----NVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLL-EDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C YF C C N ++ + G SCVC GY+ N GG ++C+KCP N + + +GW CISC +G+T C + ++ +V G+ C C T + +C C N + SCSC+ P TG C + + + +++ LG S+ S + L + A C + CQ L NMCVM++ N + ++D C L+ + + + S + + PWL+Y PQ L T L + + L+ V A+YD G F+ + + LQLC ++ + + FGT Y ++C I++S+L P+FYD YL YTD L+PIP+ + + + N + W+LTRR+ LVD + R A+ I+ S+RLV TK+G+IY P L + ++ + SA ISF V Y + S +T +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF +K L+ +H F++Q ++DIF IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ L F+EV+GFKN+ D + S + Y+AP R+ R+AV +I+ + IQ +F YER IEDK+R +VDLCS++N+S+ +L+HR FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ + R + D ++ R G RLL G + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL Q+ V +F+T+ I + IRN G NLA KTLVD RFLI
Sbjct: 118 CDFNQYFDISALSCAPCGANQRRDALGTSCVCLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPSGLTAEGKCHCPTGHILVERNVSGSLLAQATCELCDESENSFTKANALGTRCVRCEPTF-VNTSRSCSCSEPHTLTGGLCFSNTGNFHQRVISTARYGELGMSLNSEWFAKYLQATAAACWTHANLTSCQALGNMCVMNM-NSYDSTTLDA--CRLFHYIFESTAGLISVHSVPFWRQNLPWLFYGDQPGLAPQVLSTTPLPTNFSFKGQNQLKFVAASYDIRGNFIKWQ-PLEGGVLQLCPDTERRLDAAYAFGTTYQQNCEISLSKLLVDFSS-PVFYDVYLEYTDEEQHRYLWPIPV-LNLNLQHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISL--SIRLV---------PNTKNGNIYTPLLTIAYSDIDIKNAHSQSAKISFSVKYEMNQGDASVHT-------DIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFISQISIDIFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQVVFFAAFYERFIEDKIRQFVDLCSMSNVSVFLLSHRCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFQIAVSSQMRQHYDRIHETLTRRNGPARLLSSSGSTFEQSIKAYHAMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGHSFSSVLYYGNEATLLIFDLLFFCVVDLACQDFVLASFLTYLQQEIFRFIRNTVGQKNLATKTLVDERFLI 1061
BLAST of Meckelin vs. Ensembl Mouse
Match: Tmem67 (transmembrane protein 67 [Source:MGI Symbol;Acc:MGI:1923928]) HSP 1 Score: 68.5514 bits (166), Expect = 1.706e-12 Identity = 43/142 (30.28%), Postives = 64/142 (45.07%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALP 578
C YF C C N ++ + G SCVC GY+ N GG ++C+KCP N + + +GW CISC +G+T C + ++ +V G+ C C T + +C C N + SCSC+ P
Sbjct: 50 CDFNQYFDISALSCAPCGANQRRDALGTSCVCLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPSGLTAEGKCHCPTGHILVERNVSGSLLAQATCELCDESENSFTKANALGTRCVRCEPTF-VNTSRSCSCSEP 190
BLAST of Meckelin vs. UniProt/SwissProt
Match: sp|Q8BR76|MKS3_MOUSE (Meckelin OS=Mus musculus OX=10090 GN=Tmem67 PE=1 SV=2) HSP 1 Score: 494.582 bits (1272), Expect = 8.868e-158 Identity = 338/988 (34.21%), Postives = 500/988 (50.61%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACA------TNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACLT------KSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV-QPSGSYTSGKTAGY-----PWLYY-----TIPQTL--TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPF----QTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYY----NVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLL-EDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C YF C C N ++ + G SCVC GY+ N GG ++C+KCP N + + +GW CISC +G+T C + ++ +V G+ C C T + +C C N + SCSC+ P TG C + + + +++ LG S+ S + L + A C + CQ L NMCVM++ N + ++D C L+ + + + S + + PWL+Y PQ L T L + + L+ V A+YD G F+ + + LQLC ++ + + FGT Y ++C I++S+L P+FYD YL YTD L+PIP+ + + + N + W+LTRR+ LVD + R A+ I+ S+RLV TK+G+IY P L + ++ + SA ISF V Y + S +T +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF +K L+ +H F++Q ++DIF IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ L F+EV+GFKN+ D + S + Y+AP R+ R+AV +I+ + IQ +F YER IEDK+R +VDLCS++N+S+ +L+HR FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ + R + D ++ R G RLL G + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL Q+ V +F+T+ I + IRN G NLA KTLVD RFLI
Sbjct: 49 CDFNQYFDISALSCAPCGANQRRDALGTSCVCLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPSGLTAEGKCHCPTGHILVERNVSGSLLAQATCELCDESENSFTKANALGTRCVRCEPTF-VNTSRSCSCSEPHTLTGGLCFSNTGNFHQRVISTARYGELGMSLNSEWFAKYLQATAAACWTHANLTSCQALGNMCVMNM-NSYDSTTLDA--CRLFHYIFESTAGLISVHSVPFWRQNLPWLFYGDQPGLAPQVLSTTPLPTNFSFKGQNQLKFVAASYDIRGNFIKWQ-PLEGGVLQLCPDTERRLDAAYAFGTTYQQNCEISLSKLLVDFSS-PVFYDVYLEYTDEEQHRYLWPIPV-LNLNLQHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISL--SIRLV---------PNTKNGNIYTPLLTIAYSDIDIKNAHSQSAKISFSVKYEMNQGDASVHT-------DIALGVLGGLAVLSSLLKTAGWKRRVGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFISQISIDIFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQVVFFAAFYERFIEDKIRQFVDLCSMSNVSVFLLSHRCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFQIAVSSQMRQHYDRIHETLTRRNGPARLLSSSGSTFEQSIKAYHAMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGHSFSSVLYYGNEATLLIFDLLFFCVVDLACQDFVLASFLTYLQQEIFRFIRNTVGQKNLATKTLVDERFLI 992
BLAST of Meckelin vs. UniProt/SwissProt
Match: sp|P0C152|MKS3_RAT (Meckelin OS=Rattus norvegicus OX=10116 GN=Tmem67 PE=1 SV=1) HSP 1 Score: 488.804 bits (1257), Expect = 1.642e-155 Identity = 340/984 (34.55%), Postives = 494/984 (50.20%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTACATNY----RP--VNNQCQLCSNILSTNGNSSCSCALP---TGNACLT------KSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTA------GYPWLYYTIPQTL-------TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPF----QTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLL-EDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C YF C C N ++ + G SC+C GY+ N GG ++C+KCP N + + +GW CISC NG+T C S ++ +V G+ + C C N +P + +C C N + SCSC+ P TG C + + L ++ LG S S + L + A C S + CQ L NMCVM N S S C L+ V + +G + PWL+Y L T L + + L+ V A+YD G F+ + K LQLC ++ + + FGT Y ++C I++S+L P+FYD YL YTD + L+ IP+ + + + N + W+LTRR+ LVD + R A+ I+ S+RLV TK+G+IY P L + + + + S + ISF V Y + AF + +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF +K L+ +H ++Q T+DIF IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ L F+EV+GFKN+ D + S S Y+AP R+ R+AV +I+ + +Q +F YER IEDK+R +VDLCS++N+S+ +L+HR FGYYIHGRSVHG AD ++ +M L++E C++RGL ++ QT+ +A+ + R + D ++ R G RLL G ++ Y +NKFL FI+ H F +D+ E I EF + + FY D SF +VL+YG E L+ FD+L FCV DL QN V +F+T+ I + IRN G NLA KTLVD RFLI
Sbjct: 49 CDLNQYFDISALSCAPCGANQRRDALGTSCICLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPNGLTAEGKCHCPSGHILVERNVSGSLLSQATCELCDGNENSFTKPNALGTRCVRCEPTF-VNTSRSCSCSEPHISTGGLCFSNTGNFPQRLISTERYGELGMSSNSEWFTKYLQATAAACWTHSNLTSCQALGNMCVM---NMNSYDSTTFDACRLFHYVFEGAAGLTGVHSVPFWRQNLPWLFYGDQPGLASQVLSTTPLPTNFSFKGQNQLKFVAASYDIRGNFIRWQ-TVKGGVLQLCPDTERRLDAAYSFGTTYQQNCEISLSKLLADFPS-PVFYDIYLEYTDEVQHHYLWAIPV-LNLNLQHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISL--SIRLV---------PNTKNGNIYTPLLTIAYSDIDIKNAYSQSVKISFSVKYE--MNQGDAFVQT-DIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKLISQITIDIFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDPSSSLSRSLSDYAAPYSRILRYAVATTIWLVIGIVQVVFFAAFYERFIEDKIRQFVDLCSMSNVSVFLLSHRCFGYYIHGRSVHGHADTNMEDMNMNLRREAENLCSQRGLVPNTDGQTFQIAVSSQMRQHYDRIHETLTRRNGPARLLSSSGSTLEQSIKAYHAMNKFLGSFIDHVHKEMDYF-IKDKLLLEKILGMEFMEPLEKSIFYNDESHSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFVLASFLTYLQQEIFRFIRNTVGQKNLATKTLVDERFLI 992
BLAST of Meckelin vs. UniProt/SwissProt
Match: sp|Q5HYA8|MKS3_HUMAN (Meckelin OS=Homo sapiens OX=9606 GN=TMEM67 PE=1 SV=2) HSP 1 Score: 481.1 bits (1237), Expect = 1.342e-152 Identity = 332/983 (33.77%), Postives = 494/983 (50.25%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLN-QIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTACATN---YRPVN---NQCQLCSNILSTNGNSSCSCALP---TGNACLTKS----LSNSSILTY--LGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV--QPSGSYTSGKTAGY----PWLYY-----TIPQTLTQLTVDHQY-VRGET----LQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL--PPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVY-----RPGGNRLLEDGRNM------QYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C + YF CV C N +Q + G SCVC G+ N GG ++C+KCP N + + +GW+CISC + +T C ++ D+ G + C C N + VN ++C C N + SC+C+ P TG C + + L S Y +G S+TS + L S A C V + CQ L NMCVM++ N + D C L+ + +G T + + PWL+Y PQ L+ ++ + +GE L+ V A+YD G FL + + LQLC ++ T+ + FGT Y ++C I IS++ P+FYD YL YTD + +P+ + + + N + W+LTRR+ LVD T + S+ + +V T +G+IY P + + + + S + +SF V Y + +A+G+L LA L ++L+ W RR G + ++K L + ++NVFFI+ G LY+L+ FK Q S+L P + FV Y+ AF LK L+ +H ++Q T+D+F IDWERPK K A + G S VSIWRTYF+ANEWNEIQT RK + +F ++ VL F+EV+GFKN+ D + S + +Y AP + R+AV +++ IQ +F + YER IEDK+R +VDLCS++NIS+ +L+H+ FGYYIHGRSVHG AD ++ EM L++E C++RGL ++ QT+ +A+ N R + D ++ + G RLL + Y +NKFL FI+ H F +D+ E I EF + + FY D G SF +VL+YG E L+ FD+L FCV DL QN + +F+T+ I + IRN G NLA+KTLVD+RFLI
Sbjct: 49 CDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTF-VNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNM-NSYDFATFDA--CGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQ-TLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFP-TPIFYDVYLEYTDENQHQYILAVPV-LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPN-------TINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEA---HVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPK-----------GKVLKAVEGEGGVR-------SATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYF-IKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI 995
BLAST of Meckelin vs. TrEMBL
Match: A0A3Q0KQZ3 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0 Identity = 361/1052 (34.32%), Postives = 532/1052 (50.57%), Query Frame = 3
Query: 180 SSTTYFSSVDFKCVSCSVN---AKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNG---VTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACATNYRPVNNQCQLC---------------------------------SNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSME--SGCQNLANMCVMSVYNRQSAKSIDGTPCS----LYAQVQPSGSYTSGKTAGYPWLYYTIPQ---TLTQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIG-ASQLYPIPIKIGQVISDSGTTPNLLFD-------------------------QAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEV---NXXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERS--------------KFDTATDNTGDQNLIQQEKGS---------------QNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGR-----------NMQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
S+ YF+S + C CS N KQS+DGFSC+C+ GY KN GG+ V C +C + S +G CI C G SCSSC S +V+ D G+ + C AC+ N P NN C C + I ++N S S +PTG+ T L + G ++S + + + A C + + CQ LAN+C + + + + + CS L +Q P S + PWLYY+ + L + Y LQI LA + G+FLG ++A++ LQLC ++ ++ K F+F T Y ++C + + L + +Y +FYDPY+ + DS SQL PIPI+ VI SG PN++ Q W LTRRL +VDNF T+ ++ + +AS++ + V Q D DG I+ P +++ N + I+F V Y + + AF +L +AMG + LA YA++R + W RR+G+ + ++++K + A +ISN F IVI+ +S+YFL+ +K Q+++ I P+ D F + Y+ AF LK ++++H+ QCT+DIFLIDWE PK + S K++ +D T NL+ + K ++ G+S+WR ++ANEWNEIQTYRKTS + + VL+ M+VIG +N+ + D N +S Y +P R+FR A+I+SIF + IQWLF++ ++ER DKLR++ DLCSVAN+S+ + A FGYYIHG S G +DV L + L EN G KRG+ DS TY MALP+ R + +Y P N L + N Y +N++L RFI+R + +R RK FEDI DGEF+D + EG FY D+G SFG+ L+YG E L TFD LLFC+ DL+ QN + AF + +I ++R+ G N A KTLVD RFLI
Sbjct: 35 SNAEYFNSANLVCKKCSQNLTFQKQSADGFSCICQSGYKTIKNFGGNNVECTQCKDGETVSEDGLFCIPCKAGSYMAATRSCSSCPSDSVSVYLDQSGSPLASQTCLACSNNTVPDNNTCVTCRTTIRMLRASSLPESCICPNSRVDGVCFPGITPTTIGTSNSASGSSPVVPTGSDTFTLVLPAGETSSQTG--ISSKYFETQARSATAFCSTAYNNITACQLLANLCTLQLNTLYESGTGSASTCSEFRRLTSQRTPINSDYTSWVKDMPWLYYSPTKGSTVLKDTKLKTIYSPNSNLQIYLAAFKINGEFLGFQDASQGGQLQLCKDTSSRMKAAFQFATTYRQTCELNVDDLFDNEKYPLVFYDPYIYFYDSTTQTSQLIPIPIRNTAVIDKSGNFPNVITSSDQNSLNEALALGNTASAVSIEAAIQDKWELTRRLFMVDNFSGKTSTI--SKVPQLVRYASNIEIKVTSQ-----DSVTDGQIFPPIIKIDYSNFYATNDYGKGKQVQITFSVTYSSSIAKQAAFDQALRIAMGCMSTLAACYAMIRTWIWSRRAGVLRLDAMLVIKLILFAAGNISNAFLIVIYFVSIYFLIFYKVQSVYLINLPNSDEFILIYIAIAFALKCIDLIHLIAVQCTMDIFLIDWEPPKFNRNINNVNPPSNTDIQSSSRSQSLGKYNIISDMT--DNLVVRSKKELRSSLQPSSIISDQMEDTGISVWRIIYVANEWNEIQTYRKTSCLITISCVLILMQVIGLENLASSDAKSNILLNSYEYHSPQSRIFRIALILSIFSVIGIIQWLFSITLWERCFSDKLRSFADLCSVANVSVFLFAQSNFGYYIHGHSPTGRSDVDLGGITQMLSIENEGIAPKRGITPDSNDHTYRMALPSGLRQTFNRLYTPLLNLTLNADKYGKHVSAQAITNEVYHNINRYLKRFISRDDPDGLRYRIVRRKAFEDIIDGEFDDTTFEGLFYIDDGNSFGDTLYYGNEWLLFTFDALLFCLVDLLSQNLILSAFFVILIGSIFTLLRDDLGRRNTARKTLVDERFLI 1075
BLAST of Meckelin vs. TrEMBL
Match: A0A1I8I704 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0 Identity = 355/1015 (34.98%), Postives = 539/1015 (53.10%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCL----NGVTGTSCSSCDSP-NVAYSSDVLGNQFNG-NCTAC--ATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQS-------------------VTSG------------------FLQANLMDSVAMCQ--VSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSY----TSGKTAGYPWLYYTIPQTL-TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANIS--FEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDD--DSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYR---PGGNRLLEDGRNMQ------YLEVNKFLDRFINRTH-----------DPRILFRFQDRKXXXXXXXXXXXXNSL---EGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+C++ YF S C C N S+DG SCVC+ G+ T N GG+ + C+ C + +PS +GWSC++C T CS+C P N + G ++ C AC +T P C C N+L S+ + LPT T ++++S+ TY QS TSG +L +L + MC S + CQ LAN C + +Y + + + + C L+ + + + S AG PWLY++ P +L T V Q+ R T+ A Y G F+G + A + +QLC NS ++ + FGT Y SCS+ + L +Q +Y F+D YL T G QL+P+P+KI + S S PN + +ILTRR LVDN GA++ AS++ +AS +++ + S + + DG IY P + V + V S+ +SA +S F V Y+ S T F+ +L + MG+LC +A LYAI R+ + RSG FE ++L +A AC++I+NVF IVI+G S+++ ++KNQN+F+ P + ++ + Y+ A LK++E++++ + Q +DIF+IDWE+PK + R + K + V+ WRT IANEWNEIQT+RKT+ F L++VL FM +GF+N + D ++ +D Y A N +FRF + +S+F IQWL+T+LI+ER IEDKLRN+VDLCS+ANIS+ + + R FGYYIHG S HG+ADV +S+++ +KE C +RGL+ ++ QT++M LP NF D++YR G+ D + Q Y E+N FL RFI++ P + Q RK ED+ E N G FY ++GTSFG+VLFYG EL+L+TFD+L++ DLI ++T + ITW + ++ +R+ G NL+ KT+ +R+FLI
Sbjct: 36 SCATGKYFDSTRLACRDCGANQTTSADGLSCVCRSGFRITSNYGGN-IACEICSGSTVPSKDGWSCVTCPASNPKDATTGYCSACPYPLNATAEYQINGVAYSTVTCVACTSSTFVAPDGAHCIPCPNVLKMK--SAINSGLPTPTCDCTGTITSSTGATYTLQSNGGICFAVTDSTDVTLPAVRTSGIQTTISKVSSDSTTANSIYLSTHLAAAYGMCASYYSNRTACQVLANACALILYRQTNIGTGNRDACVLFNEYFSTRTEIINSNSDWKAGLPWLYHSNPSSLLTSEGVTTQFTRLSTIAFQAAVYTQNGTFVGYK-ALTEGLIQLCPNSRNEQNSAYAFGTRYTSSCSLLVDDLFDQAKYPLQFFDIYLKITSDGGTEQLFPVPVKISDLRSQSNNFPNQGSNTNDYILTRRFFLVDNVGAVST---VGSRASFVRYASKIQIDITLLSKDIY--STDGRIYPPVITVEYSDVDSNLYGTSATVSATFNVAYF---SDLTTFWEALKITMGVLCGVAALYAIFRSIAYKSRSGGENFEATIILHFIAFACSAIANVFLIVIYGASVWYFSVYKNQNIFAFFIPTEAQENNLIVYIAVAGALKLIEVIYMIITQSHIDIFMIDWEKPKADSSQLGAIGRDEV----------------KSASRTEVTYWRTVLIANEWNEIQTHRKTNRTFTLLVVLFFMSALGFENYASGDPQLSRFVVTDEYRASNVTIFRFCLAMSVFIGVGGIQWLWTLLIHERFIEDKLRNFVDLCSIANISVFIRSPRSFGYYIHGESPHGTADVGVSQLHDQFEKEKQNDCGRRGLD-NTDNQTFMMRLPPNFVSVFDNLYRNLLNTGSVAGTDAKTQQVHSIKKYHEMNNFLTRFIHKKPMEESDGVGQGGKPIEGYGVQPRKCMEDVLGMELGGNDTLRDGGIFYSEDGTSFGDVLFYGNELSLMTFDLLVYSCVDLIFKSTPLNVIITWLIGELIDFLRSQLGEINLSKKTMTNRQFLI 1021
BLAST of Meckelin vs. TrEMBL
Match: A0A267G4Y3 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034046g3 PE=4 SV=1) HSP 1 Score: 567 bits (1460), Expect = 0.000e+0 Identity = 356/1015 (35.07%), Postives = 538/1015 (53.00%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCL----NGVTGTSCSSCDSP-NVAYSSDVLGNQFNG-NCTAC--ATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQS-------------------VTSG------------------FLQANLMDSVAMCQ--VSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSY----TSGKTAGYPWLYYTIPQTL-TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANIS--FEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDD--DSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYR---PGGNRLLEDGRNMQ------YLEVNKFLDRFINRTH-----------DPRILFRFQDRKXXXXXXXXXXXXNSL---EGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+C++ YF S C C N S+DG SCVC+ G+ T N GG+ + C+ C + +PS +GWSC++C T CS+C P N + G ++ C AC +T P C C N+L S+ + LPT T ++S+S+ TY QS TSG +L +L + MC S + CQ LAN C + +Y + + + + C L+ + + + S AG PWLY++ P +L T V Q+ R T+ A Y G F+G + A + +QLC NS ++ + FGT Y SCS+ + L +Q +Y F+D YL T G QL+P+P+KI + S S PN + +ILTRR LVDN GA++ AS++ +AS +++ + S + + DG IY P + V + V S+ +SA +S F V Y+ S T F+ +L + MG+LC +A LYAI R+ + RSG FE ++L +A AC++I+NVF IVI+G S+++ ++KNQN+F+ P + + + Y+ A LK++E++++ + Q +DIF+IDWE+PK + R + K + V+ WRT IANEWNEIQT+RKT+ F L++VL FM +GF+N + D ++ +D Y A N +FRF + +S+F IQWL+T+LI+ER IEDKLRN+VDLCS+ANIS+ + + R FGYYIHG S HG+ADV +S+++ +KE C +RGL+ ++ QT++M LP NF D++YR G+ D + Q Y E+N FL RFI++ P + Q RK ED+ E N G FY ++GTSFG+VLFYG EL+L+TFD+L++ DLI ++T + ITW + ++ +R+ G NL+ KT+ +R+FLI
Sbjct: 54 SCTTGKYFDSTRLACRDCGANQTTSADGLSCVCRSGFRITSNYGGN-IACEICSGSTVPSKDGWSCVTCPASNPKDATTGYCSACPYPLNATAEYQINGVAYSTVTCVACTSSTFVAPDGAHCIPCPNVLKMK--SAINSGLPTPTCDCTGTISSSTGATYTLQSNGGICFAVTDSTDVTLPAVRTSGIQTTISKVSSDSTTANSIYLSTHLAAAYGMCASYYSNRTACQVLANACALILYRQTNIGTGNRDACVLFNEYFSTRTEIINSNSDWKAGLPWLYHSNPSSLLTSEGVTTQFTRLSTIAFQAAVYTQNGTFVGYK-ALTEGLIQLCPNSRNEQNSAYAFGTRYTSSCSLLVDDLFDQAKYPLQFFDIYLKITSDGGTEQLFPVPVKISDLRSQSNNFPNQGSNTNDYILTRRFFLVDNVGAVST---VGSRASFVRYASKIQIDITLLSKDIY--STDGRIYPPVITVEYSDVDSNLYGTSATVSATFNVAYF---SDLTTFWEALKITMGVLCGVAALYAIFRSIAYKSRSGGENFEATIILHFIAFACSAIANVFLIVIYGASVWYFSVYKNQNIFAFFIPTEAQEINLIVYIAVAGALKLIEVIYMIITQSHIDIFMIDWEKPKADSSQLGAIGRDEV----------------KSASRTEVTYWRTVLIANEWNEIQTHRKTNRTFTLLVVLFFMSALGFENYASGDPQLSRFVVTDEYRASNVTIFRFCLAMSVFIGVGGIQWLWTLLIHERFIEDKLRNFVDLCSIANISVFIRSPRSFGYYIHGESPHGTADVGVSQLHDQFEKEKQNDCGRRGLD-NTDNQTFMMRLPPNFVSVFDNLYRNLLNTGSVAGTDAKTQQVHSIKKYHEMNNFLTRFIHKKPMEESDGAGQGGKPIEGYGVQPRKCMEDVLGMELGGNDTLRDGGIFYSEDGTSFGDVLFYGNELSLMTFDLLVYSCVDLIFKSTPLNVIITWLIGELIDFLRSQLGEINLSKKTMTNRQFLI 1039
BLAST of Meckelin vs. TrEMBL
Match: A0A267H638 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034046g2 PE=4 SV=1) HSP 1 Score: 567 bits (1460), Expect = 0.000e+0 Identity = 355/1015 (34.98%), Postives = 539/1015 (53.10%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCL----NGVTGTSCSSCDSP-NVAYSSDVLGNQFNG-NCTAC--ATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQS-------------------VTSG------------------FLQANLMDSVAMCQ--VSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSY----TSGKTAGYPWLYYTIPQTL-TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANIS--FEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDD--DSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYR---PGGNRLLEDGRNMQ------YLEVNKFLDRFINRTH-----------DPRILFRFQDRKXXXXXXXXXXXXNSL---EGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+C++ YF S C C N S+DG SCVC+ G+ T N GG+ + C+ C + +PS +GWSC++C T CS+C P N + G ++ C AC +T P C C N+L S+ + LPT T ++++S+ TY QS TSG +L +L + MC S + CQ LAN C + +Y + + + + C L+ + + + S AG PWLY++ P +L T V Q+ R T+ A Y G F+G + A + +QLC NS ++ + FGT Y SCS+ + L +Q +Y F+D YL T G QL+P+P+KI + S S PN + +ILTRR LVDN GA++ AS++ +AS +++ + S + + DG IY P + V + V S+ +SA +S F V Y+ S T F+ +L + MG+LC +A LYAI R+ + RSG FE ++L +A AC++I+NVF IVI+G S+++ ++KNQN+F+ P + ++ + Y+ A LK++E++++ + Q +DIF+IDWE+PK + R + K + V+ WRT IANEWNEIQT+RKT+ F L++VL FM +GF+N + D ++ +D Y A N +FRF + +S+F IQWL+T+LI+ER IEDKLRN+VDLCS+ANIS+ + + R FGYYIHG S HG+ADV +S+++ +KE C +RGL+ ++ QT++M LP NF D++YR G+ D + Q Y E+N FL RFI++ P + Q RK ED+ E N G FY ++GTSFG+VLFYG EL+L+TFD+L++ DLI ++T + ITW + ++ +R+ G NL+ KT+ +R+FLI
Sbjct: 54 SCATGKYFDSTRLACRDCGANQTTSADGLSCVCRSGFRITSNYGGN-IACEICSGSTVPSKDGWSCVTCPASNPKDATTGYCSACPYPLNATAEYQINGVAYSTVTCVACTSSTFVAPDGAHCIPCPNVLKMK--SAINSGLPTPTCDCTGTITSSTGATYTLQSNGGICFAVTDSTDVTLPAVRTSGIQTTISKVSSDSTTANSIYLSTHLAAAYGMCASYYSNRTACQVLANACALILYRQTNIGTGNRDACVLFNEYFSTRTEIINSNSDWKAGLPWLYHSNPSSLLTSEGVTTQFTRLSTIAFQAAVYTQNGTFVGYK-ALTEGLIQLCPNSRNEQNSAYAFGTRYTSSCSLLVDDLFDQAKYPLQFFDIYLKITSDGGTEQLFPVPVKISDLRSQSNNFPNQGSNTNDYILTRRFFLVDNVGAVST---VGSRASFVRYASKIQIDITLLSKDIY--STDGRIYPPVITVEYSDVDSNLYGTSATVSATFNVAYF---SDLTTFWEALKITMGVLCGVAALYAIFRSIAYKSRSGGENFEATIILHFIAFACSAIANVFLIVIYGASVWYFSVYKNQNIFAFFIPTEAQENNLIVYIAVAGALKLIEVIYMIITQSHIDIFMIDWEKPKADSSQLGAIGRDEV----------------KSASRTEVTYWRTVLIANEWNEIQTHRKTNRTFTLLVVLFFMSALGFENYASGDPQLSRFVVTDEYRASNVTIFRFCLAMSVFIGVGGIQWLWTLLIHERFIEDKLRNFVDLCSIANISVFIRSPRSFGYYIHGESPHGTADVGVSQLHDQFEKEKQNDCGRRGLD-NTDNQTFMMRLPPNFVSVFDNLYRNLLNTGSVAGTDAKTQQVHSIKKYHEMNNFLTRFIHKKPMEESDGVGQGGKPIEGYGVQPRKCMEDVLGMELGGNDTLRDGGIFYSEDGTSFGDVLFYGNELSLMTFDLLVYSCVDLIFKSTPLNVIITWLIGELIDFLRSQLGEINLSKKTMTNRQFLI 1039
BLAST of Meckelin vs. TrEMBL
Match: A0A267H662 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034046g1 PE=4 SV=1) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 355/1015 (34.98%), Postives = 538/1015 (53.00%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCL----NGVTGTSCSSCDSP-NVAYSSDVLGNQFNG-NCTAC--ATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQS-------------------VTSG------------------FLQANLMDSVAMCQ--VSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSY----TSGKTAGYPWLYYTIPQTL-TQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANIS--FEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDD--DSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYR---PGGNRLLEDGRNMQ------YLEVNKFLDRFINRTH-----------DPRILFRFQDRKXXXXXXXXXXXXNSL---EGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
+C++ YF S C C N S+DG SCVC+ G+ T N GG+ + C+ C + +PS +GWSC++C T CS+C P N + G ++ C AC +T P C C N+L S+ + LPT T ++++S+ TY QS TSG +L +L + MC S + CQ LAN C + +Y + + + + C L+ + + + S AG PWLY++ P +L T V Q+ R T+ A Y G F+G + A + +QLC NS ++ + FGT Y SCS+ + L +Q +Y F+D YL T G QL+P+P+KI + S S PN + +ILTRR LVDN GA++ AS++ +AS +++ + S + + DG IY P + V + V S+ +SA +S F V Y+ S T F+ +L + MG+LC +A LYAI R+ + RSG FE ++L +A AC++I+NVF IVI+G S+++ ++KNQN+F+ P + + + Y+ A LK++E++++ + Q +DIF+IDWE+PK + R + K + V+ WRT IANEWNEIQT+RKT+ F L++VL FM +GF+N + D ++ +D Y A N +FRF + +S+F IQWL+T+LI+ER IEDKLRN+VDLCS+ANIS+ + + R FGYYIHG S HG+ADV +S+++ +KE C +RGL+ ++ QT++M LP NF D++YR G+ D + Q Y E+N FL RFI++ P + Q RK ED+ E N G FY ++GTSFG+VLFYG EL+L+TFD+L++ DLI ++T + ITW + ++ +R+ G NL+ KT+ +R+FLI
Sbjct: 54 SCATGKYFDSTRLACRDCGANQTTSADGLSCVCRSGFRITSNYGGN-IACEICSGSTVPSKDGWSCVTCPASNPKDATTGYCSACPYPLNATAEYQINGVAYSTVTCVACTSSTFVAPDGAHCIPCPNVLKMK--SAINSGLPTPTCDCTGTITSSTGATYTLQSNGGICFAVTDSTDVTLPAVRTSGIQTTISKVSSDSTTANSIYLSTHLAAAYGMCASYYSNRTACQVLANACALILYRQTNIGTGNRDACVLFNEYFSTRTEIINSNSDWKAGLPWLYHSNPSSLLTSEGVTTQFTRLSTIAFQAAVYTQNGTFVGYK-ALTEGLIQLCPNSRNEQNSAYAFGTRYTSSCSLLVDDLFDQAKYPLQFFDIYLKITSDGGTEQLFPVPVKISDLRSQSNNFPNQGSNTNDYILTRRFFLVDNVGAVST---VGSRASFVRYASKIQIDITLLSKDIY--STDGRIYPPVITVEYSDVDSNLYGTSATVSATFNVAYF---SDLTTFWEALKITMGVLCGVAALYAIFRSIAYKSRSGGENFEATIILHFIAFACSAIANVFLIVIYGASVWYFSVYKNQNIFAFFIPTEAQEINLIVYIAVAGALKLIEVIYMIITQSHIDIFMIDWEKPKADSSQLGAIGRDEV----------------KSASRTEVTYWRTVLIANEWNEIQTHRKTNRTFTLLVVLFFMSALGFENYASGDPQLSRFVVTDEYRASNVTIFRFCLAMSVFIGVGGIQWLWTLLIHERFIEDKLRNFVDLCSIANISVFIRSPRSFGYYIHGESPHGTADVGVSQLHDQFEKEKQNDCGRRGLD-NTDNQTFMMRLPPNFVSVFDNLYRNLLNTGSVAGTDAKTQQVHSIKKYHEMNNFLTRFIHKKPMEESDGVGQGGKPIEGYGVQPRKCMEDVLGMELGGNDTLRDGGIFYSEDGTSFGDVLFYGNELSLMTFDLLVYSCVDLIFKSTPLNVIITWLIGELIDFLRSQLGEINLSKKTMTNRQFLI 1039
BLAST of Meckelin vs. Ensembl Cavefish
Match: tmem67 (transmembrane protein 67 [Source:NCBI gene;Acc:103025602]) HSP 1 Score: 431.024 bits (1107), Expect = 9.991e-135 Identity = 304/986 (30.83%), Postives = 479/986 (48.58%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIP--SSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQF-NGNCTACATNYRPVN------NQCQLCSNILSTNGNSSCSCA---LPTGNAC-----LTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQV-QPSGSYTSGKTAGY-----PWLYYTIPQTLTQLTVDHQYV----------RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQL-SNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDI---TKDGSIYAPYLEVN-XXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL-P-PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRP-----GGNRLLEDGRN------MQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFL 2978
C YF C C N G SCVC+ G+ + G S V CQKCP + P S +G+ CI C + V C + +V G+ C AC + N+C C + N SC C +P G C L ++ ++ LG SV S + + L S A C + + CQ L NMCV+ N S S C LY + + + + + + Y PWLYY L + + + + ++++ A Y G+FL N + LQLC +++T+++ + FGT Y ++C +++S L S Q+ PLF+D +L + G +L +P+ + + +G N + W LT+RL+L+D + T I AS++++ F + T+ G +Y P + V+ + + T + +SF V Y + +A+G+L +A ++++L+ W RR + +LK L +++VFF++ G LY+++ +K Q S+L P P + FV Y+ AF LK + +H Q +VD+F IDWERP R K + + TG E + VSIWRTYF+ANEWNEIQT RK S F ++ VL F+EV+GF ++ D S++AY+ R+ R+ + +++ FIQ +F + +ER +EDK+R +VDLCS++NIS+++ +H+ +GYYIHGRSVHG AD ++ EM L++E C +RGL +S QT+ +++ + R D + P G +RL++ N Y +N+FL I+ H + + +D+ FE + EF + + FY D+ SF +VLFYG E L+ FD L FCV D+ Q+ V + +T+ I ++IRN G NL KTLVDRRFL
Sbjct: 40 CGPGGYFDISSLSCAKCGANQISGKSGLSCVCQTGFKVLTSNGRS-VTCQKCP-DSKPGVSQDGYECIQCPSAVGEDGKCQCPEGKILVERNVSGSLLQEAVCEACIGTEPDFSAPDAKGNRCVRCQDSF-INTTQSCVCGSPNIPAGGMCFPPEKLPGTVVSTVSFAQLGLSVPSAWFYSFLYSSAAACLLFTNLTACQALGNMCVL---NMHSTSSTGNDACGLYNTIYRATAAQGNSQNIPYWRTNLPWLYYGDQPGLASRVLQSEPLPVPFSFKGANKNTNIELLAAVYTVQGEFLRWEN-VRGGILQLCPDTVTRQQAAYTFGTAYKQNCVLSVSDLLSGQSE--PLFFDIFLVQQKTAGDRRLLAVPV-LNLNLQFNGQFVNQASNLNNWYLTQRLMLIDTLSGREKSLSS--TPRVIRVASNLKI--------RFQLVPNTEKGEVYPPLMSVSYSDILITDPATQTVAVSFSVEYEMDQNEARV---KTDIALGVLGGVAVVFSLLKTASWKRRISSPLIDVQTILKFLLIYAGDLASVFFVITVGTGLYWVIFYKAQQFVSVLLPLPVQEERFVTYVGCAFALKAVHFLHKLFEQLSVDVFFIDWERP-----------RGKSTKSVEVTG-------EGKTSAAPVSIWRTYFVANEWNEIQTIRKISPTFQIMAVLFFLEVVGFSSLALRDNSSELQRSAEAYTPAYSRILRYGIASTMWLCIGFIQLMFFSVFHERFVEDKIRQFVDLCSISNISVLLFSHQCYGYYIHGRSVHGHADTNMEEMNSNLKREAENLCGQRGLLPNSDIQTFEISITSRLRAQYDRILEPLSRRHGPSRLVDASANPFEQSTKAYHTMNRFLGSVIDHAHR-EMDYIVKDKLLFERLIGMEFIEPMDKSIFYNDDSYSFSDVLFYGNESTLLVFDTLFFCVVDIGSQSFVLASVLTYLQQMIFRVIRNVLGRRNLVTKTLVDRRFL 983
BLAST of Meckelin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003947.1 (pep scaffold:Pmarinus_7.0:GL483273:9876:26016:-1 gene:ENSPMAG00000003616.1 transcript:ENSPMAT00000003947.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 265.774 bits (678), Expect = 1.857e-80 Identity = 153/412 (37.14%), Postives = 230/412 (55.83%), Query Frame = 3
Query: 1779 PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRP-----GGNRLL-EDGRNMQ-----YLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
P ++ F+ YL AF LK ++ +H+ Q ++DIF DWERPK + Q + + GVS+WRT+F+ANEWNE+QT RK S + L VLLF++V+GF+N+ D V P+ +A A R+ RF V S++ + +Q +F +IYER +EDK+R +VDLCS++NISI++L +R FGYYIHGRSVHG AD ++ +M+ L+KE C++RGL + QT+ +AL R++ D + +P R+ G M+ Y +N+FL F++ H + + +D+ E + EF++ + Y D SF NVL+YG E L+ D+LLFC+ D+ Q+ IT+ + + + IR NL KTLVD RFLI
Sbjct: 2 PSEERNFIIYLSCAFALKAVQFLHLIATQVSIDIFFFDWERPKGKAR-----------------------QGAEAASARGVSVWRTFFVANEWNELQTVRKLSPMLQLFAVLLFLKVVGFENLGLRDLGVTLLPAPNADEASWSRILRFGVASSVWLVVGAVQVIFNTIIYERFVEDKIRQFVDLCSMSNISILILENRCFGYYIHGRSVHGRADTNMEDMHANLKKEEENLCSQRGLVPGTELQTFEVALTQAARLHYDRILQPLAMMAAPPRMAGPQGGPMEQSMRAYQTMNRFLCSFVDHAHK-EVDYMIRDKLLLERLTGIEFQETFDKSFLYNDESHSFSNVLYYGHEATLLLLDMLLFCIIDMGTQDFTLATIITFVVQKLFRSIRESFSRRNLVKKTLVDERFLI 389
BLAST of Meckelin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002958.1 (pep scaffold:Pmarinus_7.0:GL487132:14175:22754:1 gene:ENSPMAG00000002696.1 transcript:ENSPMAT00000002958.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 137.117 bits (344), Expect = 1.960e-35 Identity = 117/361 (32.41%), Postives = 182/361 (50.42%), Query Frame = 3
Query: 708 ESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGY---PWLYYTIPQTLTQL-TVDHQYV---RGE-----TLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRY-LPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFK 1751
+ CQ L NMCVM + A + C+ + PS G + PW+YY + L + D V RG L++VL YD G+FLG ++A LQLC ++++ + FGT Y +SC T+S+++ Q + P+FYDPYL +T G +Y +P+ + + PN DQA W+L RR LVD A+T ++ I +ASS+ + + ++ + + +IY P + V+ T S +S S +SF VVY N+S +A+G+L +LA YA+++ W RR+G+ E L+K ++SNVFFIV FG LY+L++FK
Sbjct: 23 RTACQVLGNMCVM--FMDVGALYVY---CANFFLTAPSLKQVHGIQTWFENRPWIYYMSDTAASVLASTDFPVVFSLRGSGCQESKLRLVLGMYDIRGKFLGYKSAA-GGLLQLCPGTLSRLDAAYTFGTAYAQSC--TLSRITLQELFPEPIFYDPYLQFTQG-GKEYMYALPV-LNSNLQRGDRAPNKQSDQAQWMLMRRFFLVD---ALT-------DSNTINYASSITISITMPTNNA-----NRTIYPPLITVDYTKFSKTSNDSL-KVSFSVVY-NMSQADAKL--QTEIALGVLGSLAVFYAMMQTTGWRRRAGLQMVEFSSLVKFFLILAGALSNVFFIVTFGTGLYWLIIFK 354
BLAST of Meckelin vs. Ensembl Nematostella
Match: EDO36269 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ35]) HSP 1 Score: 435.261 bits (1118), Expect = 9.833e-137 Identity = 315/999 (31.53%), Postives = 481/999 (48.15%), Query Frame = 3
Query: 177 CSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCP-----LNQIPSSNGWSCISCLNGV----TGTSCSSCDSPNVAYSSDVLGNQFNG-NCTACATNYRPVNN--QCQLC-SNILSTNGNSS-CSCA------LPTGNACLTKS--LSNSSILTYLG----QSVTSGFLQANLMDSVAMCQVSME-SGCQNLANMCVMSVYNRQ--SAKSIDGTPCSLYAQ-VQPSGSYTSGKT--------AGYPWLYYTIP-----QTLTQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVN----XXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPP--DDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYS--PSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGRN-----------MQYLEVNKFLDRFINRTHDPRIL-FRFQDRKXXXXXXXXXXXXNSLE-GRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
CSS Y+ +CV C QS D SC C+ G+ TK+ GG +LC+ C +N+ S +GWSC+ C N V T + C+ C A + G + + C C ++ PV+N CQ C S+ L +N +S+ C C L TG CL S + SS L + + + S F + NL +VA+C+ + + CQ L N+CV+ YN S C + V +GKT PWLYY L + + + L +++ G F G+ T S LQLC ++K F T Y SCSI + L + Y FYD YL D + +Y +P+ + + SG N DQ+ W L+RR LV+N I + I +A S+ + + +SS DG IY P++++ + +S ++SF V Y + A+G L LA ++A+++ W RR G + L+K L ++ + S ++F ++FG+SL +L+ FK Q++ L P ++ F LI AF K+L++ H+ +Q ++DIF +DWERP+ ++ T + G E + + VSIWRT+F+ANEWNEIQT RK S + L F++V+GF+++ T D ++ PS+ Y RFAV+ ++ + +QW + I+ER D L ++VDLC +AN+S+ ++ + +GYYIHGRSVHG AD ++ EM L++E C KRGLE ++ QT+ + LP FR D V P R + R Y +N+FL FI+ H R L + QD+ E I D E + L+ + D+G SF VLFYG E L+ F++L F D + + +T+ ++T+L IR+ G NLA KT+VD RFLI
Sbjct: 32 CSSNQYYQFSSLRCVDCGSGQTQSDDKLSCNCRPGFKKTKSDGGPNLLCESCSTGSGGVNRTASLDGWSCVLCPNDVGFDVTNSKCNDCPIGTTAVDRLIDGTKLSQRQCLKCVSDTSPVSNGGDCQRCHSSYLVSNASSTVCDCPSSENQYLQTGGVCLATSSVIKASSELYTVNYDDVKKINSFFFEDNLRAAVALCKQNQNFTSCQLLGNLCVLLDYNLDLYDGSSSTTDACKEFLDIVDIRTEKVTGKTIRTIFDWPVDMPWLYYKTGIQDAVDVLNRRDITQAFSANGPLTFIISRTTLNGSFAGL--LTDTSELQLCQERLSKMASANSFATTYKSSCSIPVKFLMQKPMY---FYDVYLKVQDPL----MYAVPVLV-ENYKKSGVAVNANSDQSRWQLSRRFFLVEN---IIGRNTGSNKVEVIRYAESIEMSIRLRSS-------DGEIYPPFIKIKYNSVEVKDEAIVEKTSKDVSFSVTY---TMDLETIDRDTKTAIGCLSGLAAIFALVKVASWRRRHGSEAIDCSTLIKLLMYSFGTFSTMYFWILFGLSLQWLIFFKQQSVVYRLLPMGSQENLFRDLLIVAFTFKLLDLFHVIWSQISIDIFFMDWERPRGRI------------TQPNQAG-------ESAGEESPVSIWRTFFVANEWNEIQTMRKISPELQIFATLFFLKVVGFEHLATTDPISRFTVNPST-GYIGEYSATLRFAVLTMVYLVIEGVQWFYFAFIHERFFGDSLGDFVDLCCMANVSVFIMENTLYGYYIHGRSVHGRADTNMKEMNEQLKREEEDLCGKRGLEPNTENQTFEVGLPQKFRQQYDKVVEPLTARGQQAQRRNVPSGGVERSINAYAAMNRFLCGFID--HSLRDLDYLIQDKLLLERIIDMEVREQPLDKAVLFNDDGRSFNCVLFYGNEWTLLLFELLFFAFCDYFATDFILAGVLTYIVITVLAKIRDIFGRRNLAKKTMVDERFLI 985
BLAST of Meckelin vs. Ensembl Medaka
Match: tmem67 (transmembrane protein 67 [Source:NCBI gene;Acc:101168892]) HSP 1 Score: 453.366 bits (1165), Expect = 3.876e-143 Identity = 320/989 (32.36%), Postives = 477/989 (48.23%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQV--LCQKCPLNQIP-SSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTAC---ATNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACL-----TKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKT------AGYPWLYYTIPQTLTQLTVDHQYV----------RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKI------GQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL-P-PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSV-----YRPGGNRLLE-DGRNMQYLE------VNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C +F C C N ++SS G SC+C+ GY TK+ ++V C++CP ++ + +G+ CI C + C S N+ D+ G F+ +C AC + + N+ C +T N+ C+C P G C + +++ S L S+ S + NL S A C V S + CQ L NMCVM N S + C L+ + S + S A PWLYY L + ++ V + +Q++ A Y+ G FL + +NLQLC K+ F FGT Y E+C +++++L T PLFYD ++ +L P+P + GQ I + G+ N W L+RR+ LVD + + + +R +V + T++G IY P + V+ T V S ++F V Y S A+G+L LA LY+IL+ W RR + +LK L ++NVFF V G LY+L+ +K Q S+L P P + F Y+ AF LK ++ ++ + Q TVD+FLIDWERP RSK T +E + + VSIWRTYF+ANEWNE+QT RK S F ++ VL F+EV+GF N+ D + S AY+ P + R+ + +++ +Q +F + YE +EDK+R +VDLCSV+NIS+++ +HR FGYYIHGRSVHG AD ++ EM L++E C +RGL S++ QT+ ++ N R+ D + R +RL++ N+ L+ +N FL I+ H P + + +D+ E + EF + + FY D SF +VLFYG E L+ FD L FCV DL QN V A +T+ + + IRN G NL NKTLVD+RFLI
Sbjct: 41 GCGVNEFFDISSLSCAKCGANQRKSSSGLSCICQTGYK-TKHFSSNKVPFTCEQCPPDKPAVTKDGFGCIRCPGRLDDNGNCQCPSGNILVERDINGTFFDEASCEACIGDSPAFTAPNSNGDRCERCQATFSNTFCTCQSPNVQAGGLCFPPGSFSTNVNPSVSYAQLAFSIQSAWFVENLYSSAAACLVFSNLTACQALGNMCVM---NMHSFTGLSTDACGLFNTISRSKAALSSVQDISYWRANLPWLYYGDEPGLAGQVLQNKPVPLSFSFRGTKKNTDIQLLAAVYNVRGDFLRW-DPIGGNNLQLCPEQAFKQAAAFSFGTTYQETCELSVAELLT-THPEPLFYDVFMDLGGG-DNRKLLPLPTLVQNQQYNGQFI-NQGSMQN-------WYLSRRIFLVDTLSGREKTLSSLPKVIRVATSIKIRFQLVPR-------TQEGQIYPPLVSVSYTDVLIKEANSQTLPVTFSVEYEMDQSEART---KTDTALGVLGGLAALYSILKTVSWKRRIASPLIDMETMLKFLLFYAGDLANVFFAVTVGTGLYWLIFYKAQQFVSVLLPLPAQEEQFETYIGCAFALKAVQFLNKLILQVTVDVFLIDWERP-----------RSKASRTVPAT-------KETKQEPSPVSIWRTYFVANEWNELQTVRKISPTFQIMAVLFFLEVLGFSNLALRDPWSTLERSPAAYTPPYSLILRYGLAATLWICIGLLQVIFFTVFYEHFVEDKIRQFVDLCSVSNISVLLFSHRCFGYYIHGRSVHGHADTNMEEMNNNLKRERESLCGQRGLLSNTDIQTFQVSFTNRLRLQYDRIQDSLSRRNRPSRLIDASAANLSELQFKAYNTMNHFLASVIDHAH-PEMDYIVKDKLTLERVIGMEFLEPIDKSIFYNDERHSFSDVLFYGNEATLLIFDTLFFCVVDLGSQNFVLAAVLTYLQQAMFRFIRNTLGRRNLVNKTLVDQRFLI 985
BLAST of Meckelin vs. Ensembl Medaka
Match: tmem67 (transmembrane protein 67 [Source:NCBI gene;Acc:101168892]) HSP 1 Score: 444.121 bits (1141), Expect = 5.749e-140 Identity = 314/978 (32.11%), Postives = 462/978 (47.24%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQV--LCQKCPLNQIP-SSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTAC---ATNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACL-----TKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKT------AGYPWLYYTIPQTLTQLTVDHQYV----------RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKI------GQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSIL-P-PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGRNMQYLEVNKF-LDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C +F C C N ++SS G SC+C+ GY TK+ ++V C++CP ++ + +G+ CI C + C S N+ D+ G F+ +C AC + + N+ C +T N+ C+C P G C + +++ S L S+ S + NL S A C V S + CQ L NMCVM N S + C L+ + S + S A PWLYY L + ++ V + +Q++ A Y+ G FL + +NLQLC K+ F FGT Y E+C +++++L T PLFYD ++ +L P+P + GQ I + G+ N W L+RR+ LVD + + + +R +V + T++G IY P + V+ T V S ++F V Y S A+G+L LA LY+IL+ W RR + +LK L ++NVFF V G LY+L+ +K Q S+L P P + F Y+ AF LK ++ ++ + Q TVD+FLIDWERP RSK T +E + + VSIWRTYF+ANEWNE+QT RK S F ++ VL F+EV+GF N+ D + S AY+ P + R+ + +++ +Q +F + YE +EDK+R +VDLCSV+NIS+++ +HR FGYYIHGRSVHG AD ++ EM L++E C +RGL S++ QT+ ++ N R+ D + + L M Y+ +K L+R I I D+ FY D SF +VLFYG E L+ FD L FCV DL QN V A +T+ + + IRN G NL NKTLVD+RFLI
Sbjct: 41 GCGVNEFFDISSLSCAKCGANQRKSSSGLSCICQTGYK-TKHFSSNKVPFTCEQCPPDKPAVTKDGFGCIRCPGRLDDNGNCQCPSGNILVERDINGTFFDEASCEACIGDSPAFTAPNSNGDRCERCQATFSNTFCTCQSPNVQAGGLCFPPGSFSTNVNPSVSYAQLAFSIQSAWFVENLYSSAAACLVFSNLTACQALGNMCVM---NMHSFTGLSTDACGLFNTISRSKAALSSVQDISYWRANLPWLYYGDEPGLAGQVLQNKPVPLSFSFRGTKKNTDIQLLAAVYNVRGDFLRW-DPIGGNNLQLCPEQAFKQAAAFSFGTTYQETCELSVAELLT-THPEPLFYDVFMDLGGG-DNRKLLPLPTLVQNQQYNGQFI-NQGSMQN-------WYLSRRIFLVDTLSGREKTLSSLPKVIRVATSIKIRFQLVPR-------TQEGQIYPPLVSVSYTDVLIKEANSQTLPVTFSVEYEMDQSEART---KTDTALGVLGGLAALYSILKTVSWKRRIASPLIDMETMLKFLLFYAGDLANVFFAVTVGTGLYWLIFYKAQQFVSVLLPLPAQEEQFETYIGCAFALKAVQFLNKLILQVTVDVFLIDWERP-----------RSKASRTVPAT-------KETKQEPSPVSIWRTYFVANEWNELQTVRKISPTFQIMAVLFFLEVLGFSNLALRDPWSTLERSPAAYTPPYSLILRYGLAATLWICIGLLQVIFFTVFYEHFVEDKIRQFVDLCSVSNISVLLFSHRCFGYYIHGRSVHGHADTNMEEMNNNLKRERESLCGQRGLLSNTDIQTFQVSFTNRLRLQYDRIQ----DSLSRAHPEMDYIVKDKLTLERVIGMEFLEPI-----DKSI-----------------FYNDERHSFSDVLFYGNEATLLIFDTLFFCVVDLGSQNFVLAAVLTYLQQAMFRFIRNTLGRRNLVNKTLVDQRFLI 949
BLAST of Meckelin vs. Ensembl Medaka
Match: tmem67 (transmembrane protein 67 [Source:NCBI gene;Acc:101168892]) HSP 1 Score: 416.387 bits (1069), Expect = 1.490e-129 Identity = 307/988 (31.07%), Postives = 461/988 (46.66%), Query Frame = 3
Query: 174 ACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQV--LCQKCPLNQIP-SSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFN-GNCTAC---ATNYRPVNNQCQLCSNILSTNGNSSCSCALP---TGNACL-----TKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQV-SMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKT------AGYPWLYYTIPQTLTQLTVDHQYV----------RGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKI------GQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXA-NISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILP-PDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSV-----YRPGGNRLLE-DGRNMQYLE------VNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
C +F C C N ++SS G SC+C+ GY TK+ ++V C++CP ++ + +G+ CI C + C S N+ D+ G F+ +C AC + + N+ C +T N+ C+C P G C + +++ S L S+ S + NL S A C V S + CQ L NMCVM N S + C L+ + S + S A PWLYY L + ++ V + +Q++ A Y+ G FL + +NLQLC K+ F FGT Y E+C +++++L T PLFYD ++ +L P+P + GQ I + G+ N W L+RR+ LVD + + + +R +V + T++G IY P + V+ T V S ++F V Y S A+G+L LA LY+IL+ W RR +A + F+ + +LP P + F Y+ AF LK ++ ++ + Q TVD+FLIDWERP RSK T +E + + VSIWRTYF+ANEWNE+QT RK S F ++ VL F+EV+GF N+ D + S AY+ P + R+ + +++ +Q +F + YE +EDK+R +VDLCSV+NIS+++ +HR FGYYIHGRSVHG AD ++ EM L++E C +RGL S++ QT+ ++ N R+ D + R +RL++ N+ L+ +N FL I+ H P + + +D+ E + EF + + FY D SF +VLFYG E L+ FD L FCV DL QN V A +T+ + + IRN G NL NKTLVD+RFLI
Sbjct: 41 GCGVNEFFDISSLSCAKCGANQRKSSSGLSCICQTGYK-TKHFSSNKVPFTCEQCPPDKPAVTKDGFGCIRCPGRLDDNGNCQCPSGNILVERDINGTFFDEASCEACIGDSPAFTAPNSNGDRCERCQATFSNTFCTCQSPNVQAGGLCFPPGSFSTNVNPSVSYAQLAFSIQSAWFVENLYSSAAACLVFSNLTACQALGNMCVM---NMHSFTGLSTDACGLFNTISRSKAALSSVQDISYWRANLPWLYYGDEPGLAGQVLQNKPVPLSFSFRGTKKNTDIQLLAAVYNVRGDFLRW-DPIGGNNLQLCPEQAFKQAAAFSFGTTYQETCELSVAELLT-THPEPLFYDVFMDLGGG-DNRKLLPLPTLVQNQQYNGQFI-NQGSMQN-------WYLSRRIFLVDTLSGREKTLSSLPKVIRVATSIKIRFQLVPR-------TQEGQIYPPLVSVSYTDVLIKEANSQTLPVTFSVEYEMDQSEART---KTDTALGVLGGLAALYSILKTVSWKRR--------------IASPLIDMEAQQFVSV------------------LLPLPAQEEQFETYIGCAFALKAVQFLNKLILQVTVDVFLIDWERP-----------RSKASRTVPAT-------KETKQEPSPVSIWRTYFVANEWNELQTVRKISPTFQIMAVLFFLEVLGFSNLALRDPWSTLERSPAAYTPPYSLILRYGLAATLWICIGLLQVIFFTVFYEHFVEDKIRQFVDLCSVSNISVLLFSHRCFGYYIHGRSVHGHADTNMEEMNNNLKRERESLCGQRGLLSNTDIQTFQVSFTNRLRLQYDRIQDSLSRRNRPSRLIDASAANLSELQFKAYNTMNHFLASVIDHAH-PEMDYIVKDKLTLERVIGMEFLEPIDKSIFYNDERHSFSDVLFYGNEATLLIFDTLFFCVVDLGSQNFVLAAVLTYLQQAMFRFIRNTLGRRNLVNKTLVDQRFLI 952
BLAST of Meckelin vs. Planmine SMEST
Match: SMESG000043913.1 (SMESG000043913.1) HSP 1 Score: 1890.55 bits (4896), Expect = 0.000e+0 Identity = 946/947 (99.89%), Postives = 946/947 (99.89%), Query Frame = 3
Query: 141 KCQTNIANVLGACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFNGNCTACATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGYPWLYYTIPQTLTQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSIGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNXXXXXXXXXXXXANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKXXXXXXXXXXXXNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 2981
KCQTNIANVLGACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFNGNCTACATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGYPWLYYTIPQTLTQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDS GASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNLTTVSSSSLTSSANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKFFEDIFDGEFEDNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI
Sbjct: 16 KCQTNIANVLGACSSTTYFSSVDFKCVSCSVNAKQSSDGFSCVCKDGYYFTKNGGGSQVLCQKCPLNQIPSSNGWSCISCLNGVTGTSCSSCDSPNVAYSSDVLGNQFNGNCTACATNYRPVNNQCQLCSNILSTNGNSSCSCALPTGNACLTKSLSNSSILTYLGQSVTSGFLQANLMDSVAMCQVSMESGCQNLANMCVMSVYNRQSAKSIDGTPCSLYAQVQPSGSYTSGKTAGYPWLYYTIPQTLTQLTVDHQYVRGETLQIVLATYDSAGQFLGIRNATKDSNLQLCDNSITKRKETFRFGTHYLESCSITISQLSNQTRYLPLFYDPYLSYTDSTGASQLYPIPIKIGQVISDSGTTPNLLFDQAAWILTRRLLLVDNFGAITAPFQTNRTASYITFASSMRLVVVKQSSGSFDITKDGSIYAPYLEVNLTTVSSSSLTSSANISFEVVYYNVSSYTTAFYYSLSVAMGILCALAGLYAILRAFFWCRRSGISGFEGLVLLKTLAHACASISNVFFIVIFGISLYFLLLFKNQNLFSILPPDDDSFFVAYLISAFLLKMLEIVHIFVAQCTVDIFLIDWERPKHQLAPKKIFERSKFDTATDNTGDQNLIQQEKGSQNNGVSIWRTYFIANEWNEIQTYRKTSLIFNLIIVLLFMEVIGFKNITTFDCFVNYSPSSDAYSAPNYRMFRFAVIVSIFGITAFIQWLFTVLIYERVIEDKLRNYVDLCSVANISIIVLAHRQFGYYIHGRSVHGSADVSLSEMYGALQKENAGSCAKRGLESDSMQQTYIMALPNNFRINLDSVYRPGGNRLLEDGRNMQYLEVNKFLDRFINRTHDPRILFRFQDRKFFEDIFDGEFEDNSLEGRFYRDNGTSFGNVLFYGQELALVTFDILLFCVTDLILQNTVFDAFITWGLVTILKMIRNGCGAHNLANKTLVDRRFLI 962 The following BLAST results are available for this feature:
BLAST of Meckelin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Meckelin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Meckelin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of Meckelin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Meckelin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Meckelin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 3
BLAST of Meckelin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of Meckelin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Meckelin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Meckelin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Meckelin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Meckelin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Meckelin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of Meckelin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008957 ID=SMED30008957|Name=Meckelin|organism=Schmidtea mediterranea sexual|type=transcript|length=2997bpback to top protein sequence of SMED30008957-orf-1 >SMED30008957-orf-1 ID=SMED30008957-orf-1|Name=SMED30008957-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=963bp MIHFIFLILNLSIFIKCQTNIANVLGACSSTTYFSSVDFKCVSCSVNAKQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
