A-kinase anchor protein 14

Overview
NameA-kinase anchor protein 14
Smed IDSMED30008947
Length (bp)788
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of A-kinase anchor protein 14 (SMED30008947) t-SNE clustered cells

Violin plots show distribution of expression levels for A-kinase anchor protein 14 (SMED30008947) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of A-kinase anchor protein 14 (SMED30008947) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for A-kinase anchor protein 14 (SMED30008947) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30008947

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30008947SMESG000043586.1 SmedASXL_011477SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Human
Match: AKAP14 (A-kinase anchoring protein 14 [Source:HGNC Symbol;Acc:HGNC:24061])

HSP 1 Score: 74.7146 bits (182), Expect = 1.144e-15
Identity = 39/123 (31.71%), Postives = 62/123 (50.41%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENK 656
            +  I W +   FT E G  +I +Y +K     KCW   ++++  ++      Y Y V WS  T   P+  ++   YF +  SK +P + PI V++  +   LVHRPG   FRE+W K + + K
Sbjct:   62 LKNIKWMTHGEFTVEKGLKQIDEYFSKCVS-KKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMKVSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAK 183
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Zebrafish
Match: si:dkeyp-81f3.4 (si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-129])

HSP 1 Score: 93.5893 bits (231), Expect = 2.279e-23
Identity = 54/133 (40.60%), Postives = 75/133 (56.39%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDK-CWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            I  I W S   FT E+GE +I  Y+  TW+     W + + ++   E + +  Y+Y+V WS PT   PIP  T  VYF +  SK++P + P+NV F IES+   H PGQ  FRE WL  VIE+K  +   V+F
Sbjct:   25 IKNIDWISCKDFTIEIGEQQIRQYMA-TWEIQHPGWLYSLTFLQQAELEFKTQYQYEVQWSIPTRFTPIPRATTRVYFIIEISKIKPDSLPVNVHFIIESNRSKHVPGQMRFREKWLMDVIESKTLLLDTVDF 156
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Xenopus
Match: AKAP14 (A-kinase anchoring protein 14 [Source:NCBI gene;Acc:100491740])

HSP 1 Score: 112.849 bits (281), Expect = 2.627e-30
Identity = 57/120 (47.50%), Postives = 75/120 (62.50%), Query Frame = 3 
Query:  297 IVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENK 656
            I W     FT ELG  +I +YI+ TW++ + W  C D++  EE +    Y Y+  WS PT RKPIP  T  VYF +  SKV+P + P+ V F  ES+ LVHRPGQ+ FRE WLK +IE+K
Sbjct:   49 ITWIPCQDFTIELGMKQIEEYIS-TWEFHESWLHCTDFLNEEEVEFSKLYHYRTRWSIPTRRKPIPRATACVYFTIQISKVKPLSLPVEVFFIFESTRLVHRPGQSRFREKWLKDIIESK 167
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Mouse
Match: Akap14 (A kinase (PRKA) anchor protein 14 [Source:MGI Symbol;Acc:MGI:3618288])

HSP 1 Score: 82.0333 bits (201), Expect = 2.405e-17
Identity = 39/123 (31.71%), Postives = 70/123 (56.91%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENK 656
            I  I W +   FTPE G  +I  +++ TW++   W +  D++  ++     +Y Y+V WS PT  +P+  ++    F +  +K +P + P++V++  E+S L+ RPG+  FRE WL+ + E K
Sbjct:  403 IKNIKWLTHGEFTPEKGRKQIEKFVS-TWEFQNRWVYYADFIEKKDLIHSYHYIYRVRWSAPTAVRPMARVSANALFTIKFNKSKPADMPVDVSYIFENSELLQRPGEIRFREQWLRDITETK 524
[Close]
BLAST of A-kinase anchor protein 14 vs. UniProt/SwissProt
Match: sp|O35817|AKA14_RAT (A-kinase anchor protein 14 OS=Rattus norvegicus OX=10116 GN=Akap14 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.652e-16
Identity = 39/123 (31.71%), Postives = 70/123 (56.91%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENK 656
            I  I W +   FT E G  +I  +++ TW++   W +  D++  ++     +Y Y+V WS PT  +P+  ++   YF +  +K +P + P++V++  E+S L+ RPG+  FRE WL+ + E K
Sbjct:  369 IKNIKWLTHGEFTAEKGRKQIEKFVS-TWEFQNRWVYYADFIEKKDLIHSFHYIYRVRWSAPTAVRPMARVSANAYFTIKFNKSKPADMPVDVSYIFENSELLQRPGKIRFREQWLRDITETK 490
[Close]
BLAST of A-kinase anchor protein 14 vs. UniProt/SwissProt
Match: sp|Q86UN6|AKA28_HUMAN (A-kinase anchor protein 14 OS=Homo sapiens OX=9606 GN=AKAP14 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.494e-15
Identity = 39/123 (31.71%), Postives = 62/123 (50.41%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENK 656
            +  I W +   FT E G  +I +Y +K     KCW   ++++  ++      Y Y V WS  T   P+  ++   YF +  SK +P + PI V++  +   LVHRPG   FRE+W K + + K
Sbjct:   62 LKNIKWMTHGEFTVEKGLKQIDEYFSKCVS-KKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMKVSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAK 183
[Close]
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Match: C3YK33 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_280477 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 3.239e-34
Identity = 62/151 (41.06%), Postives = 92/151 (60.93%), Query Frame = 3 
Query:  231 TQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            TQK+       +       +  I WP +  F  +LG+ KI ++I KTW Y+  W +CID++  E+ + +  Y+Y+V WS PT RKPIP  T  VYF +  SK++P++ P+ V +  E++ LVHRPGQ+ FRE WLK +IE+K     +VNF
Sbjct:    4 TQKYGYYDYLMLEDKEDYAVKNITWPKAEEFDIKLGDEKINEFI-KTWDYEGSWLYCIDFLQEEDHEYDKRYRYRVRWSIPTRRKPIPRATACVYFTIEVSKIKPKHFPVQVYYVFETNKLVHRPGQSRFREKWLKDIIESKIIAMEMVNF 153
[Close]
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Match: A0A210Q6P0 (A-kinase anchor protein 14 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20510 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 5.634e-33
Identity = 60/147 (40.82%), Postives = 90/147 (61.22%), Query Frame = 3 
Query:  243 NIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            N    +F+   N   +  + W +   FT E  E KI D+I KTWQY+  W++CID++G +    +  Y+++V WS PT RKPIP  T  VYF+   S ++P++ P++V F  E + LVH+PGQ+ FRE WLK +IE+K  +  +V F
Sbjct:   49 NSRQSTFVKEEN-YEVKDVKWLTIDEFTEEKAEQKIHDFI-KTWQYENSWQYCIDFLGEDPHDYDTRYRFRVRWSIPTRRKPIPRATACVYFSFVVSTIKPKHYPVDVLFVFEGNRLVHKPGQSRFREKWLKDIIESKVMMMQMVEF 193
[Close]
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Match: A0A0B6Z8J2 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF51222 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 5.905e-33
Identity = 68/186 (36.56%), Postives = 106/186 (56.99%), Query Frame = 3 
Query:  126 NTCYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            N   D+  R + ++    EK  E  R+L    ++  +K            +M  I  I W S   FT E G+ KI ++I KTW+Y+  W +C+D++ +E+ + +  Y Y+V WS PT R+PIP  T +VYF    SK+RP+  P+ V +  E++ L+H+PG++ FRE WLK +IE+K K+ S V F
Sbjct:   14 NGFIDMAFRRLETSMSDREKSFESIRQLSTSREITLRK-----------EDMYEIENIQWLSIEEFTIERGQEKIHEFI-KTWKYENSWLYCVDFLTVEDCEFDRRYFYRVRWSIPTRRRPIPRATASVYFTYVVSKIRPRTSPVEVFYVFETNRLIHQPGKSRFREMWLKDIIESKVKVISRVTF 187
[Close]
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Match: R7UZM2 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_223104 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 7.571e-32
Identity = 67/187 (35.83%), Postives = 111/187 (59.36%), Query Frame = 3 
Query:  126 NTCYDILNRSIASAAETIEKENEESRKLLDRNKL-LTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
             T +++++  I SA   +E EN+  ++ +   +L L++  +      + H N   +  I W +   F+ E  E KI D+I KTW+Y   W FC+D++  +E + ++ Y+++V WS PT RKPIP  T +VYF  + SK++P+  P+ V +  E++ LVHRPG + FRE WLK +IE+K  +   V+F
Sbjct:   10 TTAHNLVDTVIESALRKLEDENKIRKQAIAAIRLDLSRSASAEPPPPVVHENYE-VENIEWLTIEEFSIEKAEKKINDFI-KTWEYQDSWLFCVDFLREDEFEFDVRYRFRVQWSIPTRRKPIPRSTASVYFTFHVSKIKPKTYPVEVYYIFETNRLVHRPGFSRFREKWLKDIIESKVLVMDAVDF 194
[Close]
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Match: W4Y5U4 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 7.675e-32
Identity = 60/132 (45.45%), Postives = 83/132 (62.88%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            IP I W +   F  +LG +K LD   KTW+YD  W +CID++  E+DK    Y+Y+V WS PT RKP+P  T  VYF L  SK++P+  P++V +  ES+ LVH+PGQ+ FR+ WLK  I+ K  +   V F
Sbjct:   61 IPNIEWMTIEEFNIDLGTTK-LDEFVKTWEYDNSWLYCIDFLREEDDKYCKKYRYQVRWSIPTRRKPVPRATACVYFTLEVSKIKPKTFPVSVYYVFESNRLVHQPGQSRFRDRWLKDTIDTKILVMDTVTF 191
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Cavefish
Match: si:dkeyp-81f3.4 (si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-129])

HSP 1 Score: 93.2041 bits (230), Expect = 1.239e-22
Identity = 45/111 (40.54%), Postives = 64/111 (57.66%), Query Frame = 3 
Query:  303 WPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWL 635
            W S   FT E G ++I DY+ +TW+    W++ I ++   E   +  + Y+ LW +PT R PIP  T AVYF +   K R    P+ V FT+ES+  +H PG+T FRE WL
Sbjct:   79 WGSCRDFTVERGRARIEDYM-RTWEIHPSWRYSISFLQERELDFQKQFLYRALWGRPTSRAPIPRSTAAVYFIITAPKTRDPALPVEVRFTVESNRTLHEPGRTRFREKWL 188
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Nematostella
Match: EDO43483 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYL6])

HSP 1 Score: 122.094 bits (305), Expect = 2.406e-34
Identity = 62/132 (46.97%), Postives = 81/132 (61.36%), Query Frame = 3 
Query:  288 IPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            IP + W S+  FT   G  KI ++I KTW+ D  W +CID++  E+      Y+Y V WS PT RKPIP  T +VYF L  SK +P   P+ V F  E+  LVHRPG+T FRE WLK +IE+K K+ + V F
Sbjct:   68 IPNVKWMSAESFTVAGGLVKIEEFI-KTWERDGSWLYCIDFLREEDHPYSKRYRYAVKWSIPTRRKPIPRATASVYFTLEVSKFKPTTFPVAVYFIFETHRLVHRPGKTKFREKWLKDIIESKIKMMNAVYF 198
[Close]
BLAST of A-kinase anchor protein 14 vs. Ensembl Medaka
Match: si:dkeyp-81f3.4 (si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-129])

HSP 1 Score: 78.5666 bits (192), Expect = 1.329e-17
Identity = 41/118 (34.75%), Postives = 68/118 (57.63%), Query Frame = 3 
Query:  321 FTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSL 674
            FT E G+ +I + + +TW    CW+  +D++ + E +    + Y+  +S PT R+PI   T +V+F +  SK  P+  P+ V F +ES+ LV+  G+  FRE WL  VI++K +   L
Sbjct:   52 FTVEFGKEQI-ETLIETWGVRSCWRHSLDFLYVTELEHCGVFHYRARFSAPTARQPISQ-TASVFFGVEVSKFAPRATPLEVHFVVESNRLVNTSGKHRFRETWLTDVIDSKKRFSFL 167
[Close]
BLAST of A-kinase anchor protein 14 vs. Planmine SMEST
Match: SMESG000043586.1 (SMESG000043586.1)

HSP 1 Score: 431.409 bits (1108), Expect = 1.599e-155
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:   54 MNDQTEDELLFPPLFQSVSMQNQYNTCYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            MNDQTEDELLFPPLFQSVS++NQYNTCYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKE+NYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF
Sbjct:    1 MNDQTEDELLFPPLFQSVSIRNQYNTCYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEINYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 210
[Close]
BLAST of A-kinase anchor protein 14 vs. Planmine SMEST
Match: SMESG000061885.1 (SMESG000061885.1)

HSP 1 Score: 122.865 bits (307), Expect = 1.384e-33
Identity = 61/199 (30.65%), Postives = 120/199 (60.30%), Query Frame = 3 
Query:   99 QSVSMQNQYNT----CYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            +++ +  QY++     + ++++++ +A   +E E++E+   +D N    QK ++ ++        + +  I WPS A FT +LGE KI +++   W+  + W  CIDY+G  E + +  ++Y+V +S PT R PIP+ + +VYF +  SK++P +  ++V + +ESS L+HRPG+ +F E WL+++I++K  +    +F
Sbjct:   65 KNIQISGQYDSSEQEAFLLVDKALNNAIRRLEDESKENLNKIDGNSK-NQKLHLANEK-------ISVANIQWPSIATFTEQLGERKINEFVI-LWRLGEDWLHCIDYLGFLETRFDKRHRYRVRFSIPTSRHPIPVASASVYFTIVASKIKPPHRIVDVLYVVESSHLIHRPGEYIFCELWLRRIIDHKYALIKFFDF 254
[Close]
BLAST of A-kinase anchor protein 14 vs. Planmine SMEST
Match: SMESG000061885.1 (SMESG000061885.1)

HSP 1 Score: 122.094 bits (305), Expect = 4.636e-33
Identity = 61/199 (30.65%), Postives = 120/199 (60.30%), Query Frame = 3 
Query:   99 QSVSMQNQYNT----CYDILNRSIASAAETIEKENEESRKLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKILDYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLTVAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIENKAKIRSLVNF 683
            +++ +  QY++     + ++++++ +A   +E E++E+   +D N    QK ++ ++        + +  I WPS A FT +LGE KI +++   W+  + W  CIDY+G  E + +  ++Y+V +S PT R PIP+ + +VYF +  SK++P +  ++V + +ESS L+HRPG+ +F E WL+++I++K  +    +F
Sbjct:  102 KNIQISGQYDSSEQEAFLLVDKALNNAIRRLEDESKENLNKIDGNSK-NQKLHLANEK-------ISVANIQWPSIATFTEQLGERKINEFVI-LWRLGEDWLHCIDYLGFLETRFDKRHRYRVRFSIPTSRHPIPVASASVYFTIVASKIKPPHRIVDVLYVVESSHLIHRPGEYIFCELWLRRIIDHKYALIKFFDF 291
The following BLAST results are available for this feature:
BLAST of A-kinase anchor protein 14 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
AKAP141.144e-1531.71A-kinase anchoring protein 14 [Source:HGNC Symbol;... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkeyp-81f3.42.279e-2340.60si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-1... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
AKAP142.627e-3047.50A-kinase anchoring protein 14 [Source:NCBI gene;Ac... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Akap142.405e-1731.71A kinase (PRKA) anchor protein 14 [Source:MGI Symb... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|O35817|AKA14_RAT3.652e-1631.71A-kinase anchor protein 14 OS=Rattus norvegicus OX... [more]
sp|Q86UN6|AKA28_HUMAN5.494e-1531.71A-kinase anchor protein 14 OS=Homo sapiens OX=9606... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C3YK333.239e-3441.06Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A210Q6P05.634e-3340.82A-kinase anchor protein 14 OS=Mizuhopecten yessoen... [more]
A0A0B6Z8J25.905e-3336.56Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
R7UZM27.571e-3235.83Uncharacterized protein OS=Capitella teleta OX=283... [more]
W4Y5U47.675e-3245.45Uncharacterized protein OS=Strongylocentrotus purp... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkeyp-81f3.41.239e-2240.54si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-1... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO434832.406e-3446.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkeyp-81f3.41.329e-1734.75si:dkeyp-81f3.4 [Source:ZFIN;Acc:ZDB-GENE-160113-1... [more]
back to top
BLAST of A-kinase anchor protein 14 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000043586.11.599e-15598.57SMESG000043586.1[more]
SMESG000061885.11.384e-3330.65SMESG000061885.1[more]
SMESG000061885.14.636e-3330.65SMESG000061885.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008947 ID=SMED30008947|Name=A-kinase anchor protein 14|organism=Schmidtea mediterranea sexual|type=transcript|length=788bp
TTGTTTAAATTCTATTTCATTATTCAATTCACGTATTATTGCTCTGCTGC
ACAATGAATGATCAAACTGAAGATGAGTTACTTTTTCCGCCATTATTTCA
ATCCGTCTCAATGCAGAATCAGTATAATACTTGCTATGACATCCTCAACA
GATCTATAGCAAGTGCAGCAGAGACAATAGAGAAAGAAAATGAAGAAAGT
CGAAAATTGCTTGACCGAAACAAACTTTTAACACAGAAATGGAATATTGG
TTCAAAGAGTTTTATTCATCATTCCAATATGCTTTTGATACCGACGATCG
TTTGGCCATCCTCAGCCATTTTCACTCCGGAATTGGGTGAATCGAAAATC
CTAGACTATATAACGAAAACTTGGCAATACGATAAATGCTGGAAGTTTTG
CATTGACTATATGGGTTTGGAAGAAGACAAAAAAGAAATGAATTACAAAT
ATAAAGTATTGTGGAGTCAACCTACGATTAGAAAGCCGATACCTTTATTG
ACAGTGGCAGTTTATTTTAATCTATACACATCAAAAGTTCGACCTCAGAA
CGAACCAATCAATGTAACGTTCACTATCGAATCATCTTGTTTGGTACACA
GACCGGGTCAGACGGTTTTCCGTGAACATTGGCTGAAGCAAGTGATAGAA
AATAAGGCGAAAATAAGGAGCTTAGTAAATTTTTAGAATGATAAAAATTA
AGTGAAAAGAAAATTCAATTACGAATTAGAAATGTGATGTGTTTAGGAAT
GTATATATATATGTGGGAAATTTGATAAAATATGGGAG
back to top

protein sequence of SMED30008947-orf-1

>SMED30008947-orf-1 ID=SMED30008947-orf-1|Name=SMED30008947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp
MNDQTEDELLFPPLFQSVSMQNQYNTCYDILNRSIASAAETIEKENEESR
KLLDRNKLLTQKWNIGSKSFIHHSNMLLIPTIVWPSSAIFTPELGESKIL
DYITKTWQYDKCWKFCIDYMGLEEDKKEMNYKYKVLWSQPTIRKPIPLLT
VAVYFNLYTSKVRPQNEPINVTFTIESSCLVHRPGQTVFREHWLKQVIEN
KAKIRSLVNF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR025663AKAP_28
Vocabulary: molecular function
TermDefinition
GO:0016301kinase activity
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 28..62
NoneNo IPR availablePANTHERPTHR35075FAMILY NOT NAMEDcoord: 72..210
IPR025663A-kinase anchor protein 28kDaPFAMPF14469AKAP28coord: 84..204
e-value: 4.1E-37
score: 126.6