Twist

Overview
NameTwist
Smed IDSMED30008927
Length (bp)1137
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Twist (SMED30008927) t-SNE clustered cells

Violin plots show distribution of expression levels for Twist (SMED30008927) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008927

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30008927h1SMcG0018604 SmedASXL_004267SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30008927h1SMcG0018604 dd_Smed_v4_17951_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30008927h1SMcG0018604 dd_Smed_v4_17951_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30008927h1SMcG0018604 KF487106.1smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30008927h1SMcG0018604 KF487106.1smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30008927h1SMcG0018604 KF487106.1smed_ncbi_20240424PMID:25254346
Scimone et al., 2014		whole organism asexual adult colorimetric in situ hybridization evidence
musculature systemSMED30008927h1SMcG0018604 KF487106.1smed_ncbi_20240424PMID:25254346
Scimone et al., 2014		whole organism asexual adult colorimetric in situ hybridization evidence
pharynx musculatureSMED30008927h1SMcG0018604 dd_Smed_v4_17951_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Twist vs. Ensembl Human
Match: TWIST2 (twist family bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:20670])

HSP 1 Score: 123.25 bits (308), Expect = 2.379e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Human
Match: TWIST2 (twist family bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:20670])

HSP 1 Score: 123.25 bits (308), Expect = 2.379e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Human
Match: TWIST2 (twist family bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:20670])

HSP 1 Score: 123.25 bits (308), Expect = 2.379e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Human
Match: TWIST2 (twist family bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:20670])

HSP 1 Score: 123.25 bits (308), Expect = 2.379e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Human
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 113.235 bits (282), Expect = 6.466e-30
Identity = 58/108 (53.70%), Postives = 74/108 (68.52%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            QSYEELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L    ++ L S         +C Y ++  + ++  ++ +    + S
Sbjct:   34 QSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQ---SDELDS------KMASCSYVAHERLSYAFSVWRMEGAWSMS 132
[Close]
BLAST of Twist vs. Ensembl Celegans
Match: hlh-8 (Twist-related protein [Source:UniProtKB/Swiss-Prot;Acc:Q11094])

HSP 1 Score: 72.7886 bits (177), Expect = 6.044e-15
Identity = 37/71 (52.11%), Postives = 51/71 (71.83%), Query Frame = 2 
Query:  518 RRKSAQSYE--ELQN--QRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQI 718
            RRK  +     E++N  QR  AN RERQRT+ LN AF+ LR++IP++PSDK+SKI TL++A  YI FL ++
Sbjct:    4 RRKGERVVRKNEVENVQQRACANRRERQRTKELNDAFTLLRKLIPSMPSDKMSKIHTLRIATDYISFLDEM 74
[Close]
BLAST of Twist vs. Ensembl Celegans
Match: lin-32 (pep chromosome:WBcel235:X:2229208:2230122:-1 gene:WBGene00003018.1 transcript:T14F9.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lin-32)

HSP 1 Score: 60.077 bits (144), Expect = 7.003e-11
Identity = 36/83 (43.37%), Postives = 52/83 (62.65%), Query Frame = 2 
Query:  500 GSSNKR--RRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPS-DKLSKIQTLKLAASYIDFLSQILHPSANN 739
            G  +K+  RR    S + L+ +R  AN RER+R  +LN A+ ELR ++P + S  KLSK +TL++A  YI+ LSQIL   + N
Sbjct:   52 GKDDKKKCRRYKTPSPQLLRMRRSAANERERRRMNTLNVAYDELREVLPEIDSGKKLSKFETLQMAQKYIECLSQILKQDSKN 134
[Close]
BLAST of Twist vs. Ensembl Celegans
Match: hlh-10 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q23579])

HSP 1 Score: 59.6918 bits (143), Expect = 3.027e-10
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 2 
Query:  503 SSNKRRRKSAQSYE-----ELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANN 739
            + N RRR S    +     ++  +R+ AN RER R Q L++ F +LR  +P     K+SK+ TLK+A+SYI +L  IL  ++N+
Sbjct:   99 TQNSRRRTSTGKIDRRMVGKMCTRRYEANARERNRVQQLSKMFDQLRVCLPIEDDAKISKLATLKVASSYIGYLGAILQENSND 182
[Close]
BLAST of Twist vs. Ensembl Celegans
Match: hlh-13 (Helix-loop-helix protein 13 [Source:UniProtKB/Swiss-Prot;Acc:Q20561])

HSP 1 Score: 58.5362 bits (140), Expect = 3.102e-10
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 2 
Query:  539 YEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILH 724
            Y E   +R  A++RER+R  S+N AF ELR  IPT P +K LSKI TL LA +YI+ L  +L 
Sbjct:   36 YCEEPEERQTASIRERKRMCSINVAFIELRNYIPTFPYEKRLSKIDTLNLAIAYINMLDDVLR 98
[Close]
BLAST of Twist vs. Ensembl Celegans
Match: hlh-15 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18590])

HSP 1 Score: 54.6842 bits (130), Expect = 1.682e-9
Identity = 26/50 (52.00%), Postives = 36/50 (72.00%), Query Frame = 2 
Query:  578 RERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQILH 724
            RER R +S N AFS+LR ++PTLP + KLSKI+ L+ + +YI FL  +L 
Sbjct:   40 RERIRVESFNMAFSQLRALLPTLPVEKKLSKIEILRFSIAYISFLDNLLQ 89
[Close]
BLAST of Twist vs. Ensembl Fly
Match: twi (gene:FBgn0003900 transcript:FBtr0071953)

HSP 1 Score: 96.6709 bits (239), Expect = 1.326e-21
Identity = 49/77 (63.64%), Postives = 59/77 (76.62%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSI-CQSNP 769
            +E  NQR +ANVRERQRTQSLN AF  L++IIPTLPSDKLSKIQTLKLA  YIDFL ++L  S  + L ++  Q +P
Sbjct:  358 DEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSP 434
[Close]
BLAST of Twist vs. Ensembl Fly
Match: twi (gene:FBgn0003900 transcript:FBtr0345653)

HSP 1 Score: 96.6709 bits (239), Expect = 1.326e-21
Identity = 49/77 (63.64%), Postives = 59/77 (76.62%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSI-CQSNP 769
            +E  NQR +ANVRERQRTQSLN AF  L++IIPTLPSDKLSKIQTLKLA  YIDFL ++L  S  + L ++  Q +P
Sbjct:  358 DEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSP 434
[Close]
BLAST of Twist vs. Ensembl Fly
Match: twi (gene:FBgn0003900 transcript:FBtr0100130)

HSP 1 Score: 96.6709 bits (239), Expect = 1.326e-21
Identity = 49/77 (63.64%), Postives = 59/77 (76.62%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSI-CQSNP 769
            +E  NQR +ANVRERQRTQSLN AF  L++IIPTLPSDKLSKIQTLKLA  YIDFL ++L  S  + L ++  Q +P
Sbjct:  358 DEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSP 434
[Close]
BLAST of Twist vs. Ensembl Fly
Match: Fer1 (gene:FBgn0037475 transcript:FBtr0334670)

HSP 1 Score: 75.485 bits (184), Expect = 2.717e-15
Identity = 48/104 (46.15%), Postives = 63/104 (60.58%), Query Frame = 2 
Query:  431 NSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILHPSANN 739
            +S + +D EN   +EK  C  +  S   RR K A    ++  QR  AN+RER+R QS+N AF  LR  IPTLP +K LSK+ TLKLA SYI FLS+++    N 
Sbjct:   51 SSGFNSDQEN---TEKTFCPFSRRSHKPRRLKCAS---QMAQQRQAANLRERRRMQSINEAFEGLRTHIPTLPYEKRLSKVDTLKLAISYITFLSEMVKKDKNG 148
[Close]
BLAST of Twist vs. Ensembl Fly
Match: Fer1 (gene:FBgn0037475 transcript:FBtr0081567)

HSP 1 Score: 70.0922 bits (170), Expect = 1.742e-13
Identity = 41/79 (51.90%), Postives = 52/79 (65.82%), Query Frame = 2 
Query:  506 SNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILHPSANN 739
            S+K RR    S  ++  QR  AN+RER+R QS+N AF  LR  IPTLP +K LSK+ TLKLA SYI FLS+++    N 
Sbjct:   67 SHKPRRLKCAS--QMAQQRQAANLRERRRMQSINEAFEGLRTHIPTLPYEKRLSKVDTLKLAISYITFLSEMVKKDKNG 143
[Close]
BLAST of Twist vs. Ensembl Zebrafish
Match: twist2 (twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235])

HSP 1 Score: 115.931 bits (289), Expect = 1.196e-30
Identity = 63/121 (52.07%), Postives = 81/121 (66.94%), Query Frame = 2 
Query:  509 NKRRRK----SAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            NKR +K    S QS+EELQNQR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L     +N ++S          C Y ++  + ++  ++ +    + S
Sbjct:   50 NKRNKKPSPSSTQSFEELQNQRVLANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLASRYIDFLCQVLQSDEMDNKMSS----------CSYVAHERLSYAFSVWRMEGAWSMS 160
[Close]
BLAST of Twist vs. Ensembl Zebrafish
Match: twist1b (twist family bHLH transcription factor 1b [Source:ZFIN;Acc:ZDB-GENE-050417-357])

HSP 1 Score: 112.849 bits (281), Expect = 9.014e-30
Identity = 52/64 (81.25%), Postives = 57/64 (89.06%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILH 724
            QS+EELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L 
Sbjct:   68 QSFEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQ 131
[Close]
BLAST of Twist vs. Ensembl Zebrafish
Match: twist1b (twist family bHLH transcription factor 1b [Source:ZFIN;Acc:ZDB-GENE-050417-357])

HSP 1 Score: 113.235 bits (282), Expect = 1.323e-29
Identity = 57/108 (52.78%), Postives = 74/108 (68.52%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            QS+EELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L    ++ L S         +C Y ++  + ++  ++ +    + S
Sbjct:   68 QSFEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQ---SDELDS------KMASCSYVAHERLSYAFSVWRMEGAWSMS 166
[Close]
BLAST of Twist vs. Ensembl Zebrafish
Match: twist1a (twist family bHLH transcription factor 1a [Source:ZFIN;Acc:ZDB-GENE-000210-6])

HSP 1 Score: 107.071 bits (266), Expect = 2.773e-27
Identity = 51/72 (70.83%), Postives = 58/72 (80.56%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            E+LQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     ++  S C
Sbjct:   73 EDLQTQRVMANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQSDELDSKMSSC 144
[Close]
BLAST of Twist vs. Ensembl Zebrafish
Match: twist1a (twist family bHLH transcription factor 1a [Source:ZFIN;Acc:ZDB-GENE-000210-6])

HSP 1 Score: 107.071 bits (266), Expect = 2.773e-27
Identity = 51/72 (70.83%), Postives = 58/72 (80.56%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            E+LQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     ++  S C
Sbjct:   73 EDLQTQRVMANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQSDELDSKMSSC 144
[Close]
BLAST of Twist vs. Ensembl Xenopus
Match: rab34 (RAB34, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-494108])

HSP 1 Score: 108.227 bits (269), Expect = 1.295e-27
Identity = 54/106 (50.94%), Postives = 73/106 (68.87%), Query Frame = 2 
Query:  539 YEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            +EELQ+QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L    ++ L S         +C Y ++  + ++  ++ +    + S
Sbjct:   67 FEELQSQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLASRYIDFLCQVLQ---SDELDS------KMASCSYVAHERLSYAFSVWRMEGAWSMS 163
[Close]
BLAST of Twist vs. Ensembl Xenopus
Match: csnk1a1 (casein kinase 1 alpha 1 [Source:Xenbase;Acc:XB-GENE-478121])

HSP 1 Score: 103.219 bits (256), Expect = 7.977e-26
Identity = 47/64 (73.44%), Postives = 57/64 (89.06%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILH 724
            +++E++  QR +ANVRERQRTQSLN AF+ELR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L 
Sbjct:   61 ETFEDVHTQRIIANVRERQRTQSLNDAFAELRKIIPTLPSDKLSKIQTLKLASRYIDFLYQVLQ 124
[Close]
BLAST of Twist vs. Ensembl Xenopus
Match: adra1d (adrenoceptor alpha 1D [Source:Xenbase;Acc:XB-GENE-6050827])

HSP 1 Score: 81.2629 bits (199), Expect = 2.325e-17
Identity = 47/86 (54.65%), Postives = 57/86 (66.28%), Query Frame = 2 
Query:  467 GSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            G E   CSP   S  KR+RKS  S   +  QR  AN RER RT S+N AF+ LR +IPT P+D KLSKI+TL+LA+SYI  L+ IL
Sbjct:   75 GDEGGCCSPAEESRGKRKRKSRLSG--VSKQRQAANARERDRTHSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLANIL 158
[Close]
BLAST of Twist vs. Ensembl Xenopus
Match: PTF1A (pancreas associated transcription factor 1a [Source:NCBI gene;Acc:100494161])

HSP 1 Score: 75.485 bits (184), Expect = 1.363e-14
Identity = 43/89 (48.31%), Postives = 57/89 (64.04%), Query Frame = 2 
Query:  467 GSEKITCSPNV--GSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILH 724
            G      SP +  GS   +RR+  +S  E+Q  R  ANVRER+R QS+N AF  LR  IPTLP +K LSK+ TL+LA  YI+FLS+++ 
Sbjct:  138 GEGGCELSPGMKGGSLVMKRRRRLRSDAEMQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQ 226
[Close]
BLAST of Twist vs. Ensembl Xenopus
Match: tcf15 (transcription factor 15 [Source:NCBI gene;Acc:549260])

HSP 1 Score: 67.3958 bits (163), Expect = 6.113e-13
Identity = 49/123 (39.84%), Postives = 67/123 (54.47%), Query Frame = 2 
Query:  503 SSNKRRR----KSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQ-SNPGFNNC--EYNSYNNIPFSNDLYTIANHQ 847
            SS KRR+          + +  QR  AN RER RTQS+N AF+ LR +IPT P D KLSKI+TL+LA+SYI  L+ +L       L   CQ   P F+      +S   +P S   + ++N +
Sbjct:   41 SSPKRRKVGGTGGTGGGQPVVKQRQAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVL------LLGEGCQDGQPCFSTVYRAKDSDGKVPRSICTFCLSNQR 157
[Close]
BLAST of Twist vs. Ensembl Mouse
Match: Twist2 (twist basic helix-loop-helix transcription factor 2 [Source:MGI Symbol;Acc:MGI:104685])

HSP 1 Score: 123.25 bits (308), Expect = 1.634e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Mouse
Match: Twist2 (twist basic helix-loop-helix transcription factor 2 [Source:MGI Symbol;Acc:MGI:104685])

HSP 1 Score: 123.25 bits (308), Expect = 1.634e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Mouse
Match: Twist1 (twist basic helix-loop-helix transcription factor 1 [Source:MGI Symbol;Acc:MGI:98872])

HSP 1 Score: 114.005 bits (284), Expect = 1.924e-29
Identity = 58/108 (53.70%), Postives = 74/108 (68.52%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            QSYEELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L    ++ L S         +C Y ++  + ++  ++ +    + S
Sbjct:  105 QSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQ---SDELDS------KMASCSYVAHERLSYAFSVWRMEGAWSMS 203
[Close]
BLAST of Twist vs. Ensembl Mouse
Match: Scx (scleraxis [Source:MGI Symbol;Acc:MGI:102934])

HSP 1 Score: 70.0922 bits (170), Expect = 1.680e-13
Identity = 38/74 (51.35%), Postives = 46/74 (62.16%), Query Frame = 2 
Query:  557 QRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGF 775
            QR  AN RER RT S+N AF+ LR +IPT P+D KLSKI+TL+LA+SYI  L  +L           C S P F
Sbjct:   79 QRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHSGPAF 152
[Close]
BLAST of Twist vs. Ensembl Mouse
Match: Tcf15 (transcription factor 15 [Source:MGI Symbol;Acc:MGI:104664])

HSP 1 Score: 67.781 bits (164), Expect = 7.137e-13
Identity = 38/75 (50.67%), Postives = 49/75 (65.33%), Query Frame = 2 
Query:  500 GSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            G  + RR  +      +  QR  AN RER RTQS+N AF+ LR +IPT P D KLSKI+TL+LA+SYI  L+ +L
Sbjct:   52 GPGSGRRASNGAGPVVVVRQRQAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYIAHLANVL 126
[Close]
BLAST of Twist vs. UniProt/SwissProt
Match: sp|Q9D030|TWST2_MOUSE (Twist-related protein 2 OS=Mus musculus OX=10090 GN=Twist2 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 7.432e-33
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAYSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. UniProt/SwissProt
Match: sp|P97831|TWST2_RAT (Twist-related protein 2 OS=Rattus norvegicus OX=10116 GN=Twist2 PE=2 SV=2)

HSP 1 Score: 123.25 bits (308), Expect = 1.143e-32
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. UniProt/SwissProt
Match: sp|Q8WVJ9|TWST2_HUMAN (Twist-related protein 2 OS=Homo sapiens OX=9606 GN=TWIST2 PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.143e-32
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            SP  G   K+   SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:   44 SPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. UniProt/SwissProt
Match: sp|O96642|TWIST_BRABE (Twist-related protein OS=Branchiostoma belcheri OX=7741 GN=TWIST PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.229e-30
Identity = 60/103 (58.25%), Postives = 77/103 (74.76%), Query Frame = 2 
Query:  509 NKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFS 817
            N+++   A+S+E+LQNQR LANVRERQRTQSLN AFS LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L   +++T T +  S      C Y ++  + ++
Sbjct:   80 NRKKTSKAESFEDLQNQRVLANVRERQRTQSLNEAFSSLRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLR--SDDTDTKMASS------CSYVAHERLSYA 174
[Close]
BLAST of Twist vs. UniProt/SwissProt
Match: sp|P13903|TWIST_XENLA (Twist-related protein OS=Xenopus laevis OX=8355 GN=twist1 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.612e-29
Identity = 63/136 (46.32%), Postives = 87/136 (63.97%), Query Frame = 2 
Query:  449 DTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            D ++PI  ++   + + GSS        QS+EELQ+QR +ANVRERQRTQSLN AFS LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L    ++ L S         +C Y ++  + ++  ++ +    + S
Sbjct:   44 DPDSPISVKRNKKASSTGSS-------PQSFEELQSQRVMANVRERQRTQSLNEAFSSLRKIIPTLPSDKLSKIQTLKLASRYIDFLCQVLQ---SDELDS------KMASCSYVAHERLSYAFSVWRMEGAWSMS 163
[Close]
BLAST of Twist vs. TrEMBL
Match: D3J1K5 (Twist OS=Schmidtea polychroa OX=50054 GN=twi PE=2 SV=1)

HSP 1 Score: 492.656 bits (1267), Expect = 1.575e-173
Identity = 238/250 (95.20%), Postives = 245/250 (98.00%), Query Frame = 2 
Query:  332 MKSEYAEFFEISNPLASSLSIGKKEPNHLWNERNSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS 1081
            MKSEYAEFFEISNPLA++LSI KKE NH+WNERNS+YMTD+EN IGS+KITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLY IANHQTTSKVNNPQILKFL+NNANVDN LMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS
Sbjct:    1 MKSEYAEFFEISNPLANTLSINKKEQNHIWNERNSAYMTDSENQIGSDKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYAIANHQTTSKVNNPQILKFLTNNANVDNTLMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS 250
[Close]
BLAST of Twist vs. TrEMBL
Match: U5YR67 (Twist protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=twist PE=2 SV=1)

HSP 1 Score: 446.047 bits (1146), Expect = 1.306e-155
Identity = 215/224 (95.98%), Postives = 220/224 (98.21%), Query Frame = 2 
Query:  398 KKEPNHLWNERNSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAW 1069
            KKE NH+WNERNS+YMTD+EN IGS+KITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLY IANHQTTSKVNNPQILKFL+NNANVDN LMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAW
Sbjct:    1 KKEQNHIWNERNSAYMTDSENQIGSDKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYAIANHQTTSKVNNPQILKFLTNNANVDNTLMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAW 224
[Close]
BLAST of Twist vs. TrEMBL
Match: A0A091CN74 (Twist-related protein 2 OS=Fukomys damarensis OX=885580 GN=H920_20078 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 9.667e-36
Identity = 88/209 (42.11%), Postives = 109/209 (52.15%), Query Frame = 2 
Query:  458 NPIGSEKITCSPNVGSSNKRRRK----SAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWA 1072
             P G       P   S    +RK    SAQS+EELQ+QR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     ++            +C Y ++  + ++  +          K+   Q LK  +   +                 L Q L  +      +  S  AHE LSYAFSVWRMEGAW+
Sbjct:   39 RPCGEPGYGQYPGSASPAPGKRKKASPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDS---------KMTSCSYVAHERLSYAFSV----------KI---QTLKLAARYIDF----------------LYQVLQSDEMDSKMTSCSYVAHERLSYAFSVWRMEGAWS 209
[Close]
BLAST of Twist vs. TrEMBL
Match: A0A2P8XME5 (Twist-related protein 2 OS=Blattella germanica OX=6973 GN=Twist2 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 2.202e-33
Identity = 86/187 (45.99%), Postives = 102/187 (54.55%), Query Frame = 2 
Query:  509 NKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAW 1069
             K RRKS QS+EELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+LH  A+           G  + E NS ++               +S   +P          +   ALMS                   ++  +S  +  AHE LSYAFSVWRMEG W
Sbjct:  104 GKVRRKS-QSFEELQTQRVMANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLHCGAD-----------GQEDTETNSGSD-------------GRSSGACSP----------STRLALMS-------------------ASAVTSSCTYMAHERLSYAFSVWRMEGDW 236
[Close]
BLAST of Twist vs. TrEMBL
Match: A0A4Y7LMB1 (EOG090X0511 OS=Daphnia similis OX=35528 GN=EOG090X0511 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 6.001e-32
Identity = 83/179 (46.37%), Postives = 102/179 (56.98%), Query Frame = 2 
Query:  539 YEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTL--TSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAW 1069
            ++++Q+QR LANVRERQRTQSLN AFS LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L      T+  +S+  S+ G        + +   S D Y  A    TS   +PQ++   + N                         G  S  HSS ++N   E LSYAFSVWRMEGAW
Sbjct:  140 HQDVQSQRVLANVRERQRTQSLNEAFSALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLRTDDEGTVDSSSVLPSSGG--------HGSATVSPDTYNCA---ATSP--DPQMIGHSTRNQ------------------------GGNSGGHSSFLAN---ECLSYAFSVWRMEGAW 278
[Close]
BLAST of Twist vs. Ensembl Cavefish
Match: twist1a (twist family bHLH transcription factor 1a [Source:ZFIN;Acc:ZDB-GENE-000210-6])

HSP 1 Score: 117.087 bits (292), Expect = 3.215e-31
Identity = 73/176 (41.48%), Postives = 96/176 (54.55%), Query Frame = 2 
Query:  353 FFEISNPLASSLSIGKKEPNHLWNERNSSYMTDTENPI--------GSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            F E S+P +   S+G           NS    D + P          S + + SP       +R  S QS+EELQ QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L    ++ L S   S      C Y ++  + ++  ++ +    + S
Sbjct:    2 FLESSSPESPVDSLG-----------NSEEEADAKQPKRGGARKRRASARSSGSPAGPGKRGKRSSSPQSFEELQTQRVMANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQ---SDELDSKVAS------CSYVAHERLSYAFSVWRMEGAWSMS 157
[Close]
BLAST of Twist vs. Ensembl Cavefish
Match: twist2 (twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235])

HSP 1 Score: 116.316 bits (290), Expect = 7.290e-31
Identity = 59/89 (66.29%), Postives = 69/89 (77.53%), Query Frame = 2 
Query:  500 GSSNKRRRKS---AQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            GS+ + ++ S   A SYEELQNQR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L     ++  S C
Sbjct:   53 GSAKRGKKASPSRAHSYEELQNQRVLANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLASRYIDFLYQVLQSDEMDSKMSSC 141
[Close]
BLAST of Twist vs. Ensembl Cavefish
Match: ENSAMXT00000026383.2 (pep primary_assembly:Astyanax_mexicanus-2.0:13:8506334:8511562:-1 gene:ENSAMXG00000025657.2 transcript:ENSAMXT00000026383.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.76 bits (260), Expect = 1.154e-26
Identity = 54/90 (60.00%), Postives = 65/90 (72.22%), Query Frame = 2 
Query:  503 SSNKRRRKSAQSY-----EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            SS  ++RK A+       E+ Q QR LANVRERQRTQSLN AF+ LR++IPTLPSDKLSKIQTLKLA+ YIDFL Q+L   A  + +  C
Sbjct:   39 SSLVKKRKKARGVNIHPPEDTQKQRVLANVRERQRTQSLNEAFASLRKMIPTLPSDKLSKIQTLKLASRYIDFLYQVLQSDATESRSYAC 128
[Close]
BLAST of Twist vs. Ensembl Cavefish
Match: twist3 (twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7])

HSP 1 Score: 102.064 bits (253), Expect = 1.127e-25
Identity = 51/89 (57.30%), Postives = 64/89 (71.91%), Query Frame = 2 
Query:  503 SSNKRRRKS----AQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            S  KR +K      Q++E++ +QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQ LKLA+ YIDFL Q+L     +   + C
Sbjct:   44 SGPKRPKKGSPLPGQAFEDVHSQRVIANVRERQRTQSLNDAFASLRKIIPTLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASC 132
[Close]
BLAST of Twist vs. Ensembl Cavefish
Match: ENSAMXT00000025720.2 (twist-related protein-like [Source:NCBI gene;Acc:103035798])

HSP 1 Score: 98.9821 bits (245), Expect = 2.810e-24
Identity = 52/114 (45.61%), Postives = 68/114 (59.65%), Query Frame = 2 
Query:  524 KSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILH---PSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            +S    EEL  QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YI FL  +L    P A               +C Y ++  + ++  ++ +    + S
Sbjct:   71 ESPPPLEELHTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLASRYIHFLCHVLQSDTPDAKGA------------SCSYVAHERLSYAFSVWRMEGAWSMS 172

HSP 2 Score: 48.1358 bits (113), Expect = 2.234e-6
Identity = 22/42 (52.38%), Postives = 26/42 (61.90%), Query Frame = 2 
Query:  947 LHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWA 1072
            +H L   L  +T     +  S  AHE LSYAFSVWRMEGAW+
Sbjct:  129 IHFLCHVLQSDTPDAKGASCSYVAHERLSYAFSVWRMEGAWS 170
[Close]
BLAST of Twist vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 114.39 bits (285), Expect = 1.195e-31
Identity = 59/109 (54.13%), Postives = 75/109 (68.81%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHP-SANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            QSYE+LQNQR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L     +N +TS          C Y ++  + ++  ++ +    + S
Sbjct:    1 QSYEDLQNQRVLANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLASRYIDFLYQVLQSDEMDNKMTS----------CSYVAHERLSYAFSVWRMEGAWSMS 99

HSP 2 Score: 45.4394 bits (106), Expect = 1.285e-6
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 2 
Query: 1016 AHETLSYAFSVWRMEGAWA 1072
            AHE LSYAFSVWRMEGAW+
Sbjct:   79 AHERLSYAFSVWRMEGAWS 97
[Close]
BLAST of Twist vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 107.842 bits (268), Expect = 3.641e-28
Identity = 53/79 (67.09%), Postives = 60/79 (75.95%), Query Frame = 2 
Query:  485 CSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQIL 721
            C    GS+       + S EE+Q QRF+ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA  YIDFL Q+L
Sbjct:   72 CGAADGSAGSAGSVCSLSGEEVQQQRFVANVRERQRTQSLNEAFASLRQIIPTLPSDKLSKIQTLKLATRYIDFLYQVL 150
[Close]
BLAST of Twist vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 107.071 bits (266), Expect = 1.020e-27
Identity = 53/79 (67.09%), Postives = 60/79 (75.95%), Query Frame = 2 
Query:  485 CSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQIL 721
            C    GS+       + S EE+Q QRF+ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA  YIDFL Q+L
Sbjct:   90 CGAADGSAGSAGSVCSLSGEEVQQQRFVANVRERQRTQSLNEAFASLRQIIPTLPSDKLSKIQTLKLATRYIDFLYQVL 168
[Close]
BLAST of Twist vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011442.1 (pep scaffold:Pmarinus_7.0:GL478554:30221:30385:-1 gene:ENSPMAG00000010415.1 transcript:ENSPMAT00000011442.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.1149 bits (209), Expect = 2.476e-21
Identity = 41/49 (83.67%), Postives = 43/49 (87.76%), Query Frame = 2 
Query:  578 RERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILH 724
            RERQRTQSLN AFS LR+IIPTLPSDKLSKIQTLKLA  YIDFL Q+L 
Sbjct:    1 RERQRTQSLNEAFSSLRQIIPTLPSDKLSKIQTLKLATRYIDFLYQVLQ 49
[Close]
BLAST of Twist vs. Ensembl Sea Lamprey
Match: scxa (scleraxis bHLH transcription factor a [Source:ZFIN;Acc:ZDB-GENE-060503-414])

HSP 1 Score: 63.1586 bits (152), Expect = 1.902e-12
Identity = 34/56 (60.71%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:  557 QRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            QR  AN RER RTQS+N AF+ LR +IPT P+D KLSK++TL LA+SYI  L   L
Sbjct:   37 QRRAANARERDRTQSVNSAFTALRTLIPTEPADRKLSKLETLLLASSYIAHLGNTL 92
[Close]
BLAST of Twist vs. Ensembl Nematostella
Match: TWIST (Predicted protein; Twist family bHLH transcription factor [Source:UniProtKB/TrEMBL;Acc:Q6S5G7])

HSP 1 Score: 95.5153 bits (236), Expect = 5.023e-24
Identity = 46/65 (70.77%), Postives = 56/65 (86.15%), Query Frame = 2 
Query:  554 NQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQIL-----HPSA 733
            +QR +ANVRERQRTQ+LN AF++LR+IIPTLPSDKLSKIQTL+LA+ YIDFL Q+L     HPS+
Sbjct:   36 DQRAIANVRERQRTQALNEAFNKLRKIIPTLPSDKLSKIQTLRLASRYIDFLCQVLGNNEKHPSS 100

HSP 2 Score: 45.8246 bits (107), Expect = 3.071e-6
Identity = 22/36 (61.11%), Postives = 26/36 (72.22%), Query Frame = 2 
Query:  968 LYGNTSTPHSSDVSNN--AHETLSYAFSVWRMEGAW 1069
            + GN     SS +S++  AHE LSYAFSVWRMEGAW
Sbjct:   90 VLGNNEKHPSSALSSSFIAHERLSYAFSVWRMEGAW 125
[Close]
BLAST of Twist vs. Ensembl Nematostella
Match: EDO43849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXI0])

HSP 1 Score: 66.2402 bits (160), Expect = 3.310e-14
Identity = 32/56 (57.14%), Postives = 43/56 (76.79%), Query Frame = 2 
Query:  557 QRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQIL 721
            QR  AN+RER+R QS+N AF  LR+ IPTLP +K LSK+ TL+LA  YI FL++++
Sbjct:    3 QRHAANLRERKRMQSINEAFEGLRKHIPTLPYEKRLSKVDTLRLAIGYIGFLTEMI 58
[Close]
BLAST of Twist vs. Ensembl Nematostella
Match: EDO42924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZZ0])

HSP 1 Score: 65.4698 bits (158), Expect = 5.759e-14
Identity = 37/56 (66.07%), Postives = 43/56 (76.79%), Query Frame = 2 
Query:  557 QRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            QR  AN RER RT S+N AF+ LR +IPT PSD KLSKI+TL+LA+SYI  LS IL
Sbjct:    2 QRQAANARERNRTHSVNAAFNALRLLIPTEPSDRKLSKIETLRLASSYIAHLSTIL 57
[Close]
BLAST of Twist vs. Ensembl Nematostella
Match: EDO40812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S669])

HSP 1 Score: 57.7658 bits (138), Expect = 3.457e-11
Identity = 29/45 (64.44%), Postives = 38/45 (84.44%), Query Frame = 2 
Query:  578 RERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFL 709
            +ER+RT+++N AF+ELR+ IP +PSD KLSKI+TLKLA SYI  L
Sbjct:    7 KERRRTENINAAFAELRKHIPNVPSDTKLSKIKTLKLAMSYIHHL 51
[Close]
BLAST of Twist vs. Ensembl Nematostella
Match: EDO46193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN3])

HSP 1 Score: 55.4546 bits (132), Expect = 2.473e-10
Identity = 29/56 (51.79%), Postives = 37/56 (66.07%), Query Frame = 2 
Query:  557 QRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            +R   N RER R Q +N AF+ELR++IPT P + KLSK + L+ A  YI FL  IL
Sbjct:    2 KRLYTNSRERWRQQHVNLAFAELRKLIPTYPPERKLSKNEILRFAMKYIKFLENIL 57
[Close]
BLAST of Twist vs. Ensembl Medaka
Match: twist2 (twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235])

HSP 1 Score: 117.472 bits (293), Expect = 2.590e-31
Identity = 57/77 (74.03%), Postives = 63/77 (81.82%), Query Frame = 2 
Query:  527 SAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            S QSYEELQNQR LANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L     ++  S C
Sbjct:   64 STQSYEELQNQRVLANVRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLASRYIDFLCQVLQSDEMDSKMSSC 140
[Close]
BLAST of Twist vs. Ensembl Medaka
Match: twist1 (twist family bHLH transcription factor 1 [Source:NCBI gene;Acc:100049283])

HSP 1 Score: 113.62 bits (283), Expect = 8.101e-30
Identity = 57/108 (52.78%), Postives = 76/108 (70.37%), Query Frame = 2 
Query:  533 QSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTS 856
            QS+EELQ+QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L    ++ L S        ++C Y ++  + ++  ++ +    + S
Sbjct:   67 QSFEELQSQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLCQVLQ---SDELDS------KLSSCSYVAHERLSYAFSVWRMEGAWSMS 165
[Close]
BLAST of Twist vs. Ensembl Medaka
Match: ENSORLT00000005014.2 (twist family bHLH transcription factor 1 [Source:NCBI gene;Acc:101173479])

HSP 1 Score: 107.457 bits (267), Expect = 2.331e-27
Identity = 50/76 (65.79%), Postives = 61/76 (80.26%), Query Frame = 2 
Query:  530 AQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            A+S+++LQ QR +AN+RERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLAA YIDFL Q+L     +   + C
Sbjct:   82 ARSFDDLQTQRVMANIRERQRTQSLNEAFTSLRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDARGTSC 157
[Close]
BLAST of Twist vs. Ensembl Medaka
Match: twist3 (twist-related protein 2-like [Source:NCBI gene;Acc:101165973])

HSP 1 Score: 102.064 bits (253), Expect = 3.010e-25
Identity = 49/78 (62.82%), Postives = 59/78 (75.64%), Query Frame = 2 
Query:  524 KSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            +S   +EEL +QR +ANVRERQRTQSLN AF+ LR+IIPTLPSDKLSKIQ LKLA+ YIDFL Q+L     +   + C
Sbjct:   86 RSEPPFEELHSQRVIANVRERQRTQSLNDAFASLRKIIPTLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASC 163
[Close]
BLAST of Twist vs. Ensembl Medaka
Match: ENSORLT00000026838.1 (twist-related protein 2-like [Source:NCBI gene;Acc:101174995])

HSP 1 Score: 97.8265 bits (242), Expect = 1.535e-23
Identity = 46/74 (62.16%), Postives = 57/74 (77.03%), Query Frame = 2 
Query:  536 SYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSIC 757
            + E+   QR +AN+RERQRTQSLN AF+ LR+IIPTLPSDKLSKIQTLKLA+ YIDFL Q+L     ++  + C
Sbjct:  113 ALEDPHAQRVIANIRERQRTQSLNEAFASLRKIIPTLPSDKLSKIQTLKLASRYIDFLYQVLQSDQMDSKLAGC 186
[Close]
BLAST of Twist vs. Planmine SMEST
Match: SMESG000026385.1 (SMESG000026385.1)

HSP 1 Score: 507.679 bits (1306), Expect = 0.000e+0
Identity = 246/250 (98.40%), Postives = 249/250 (99.60%), Query Frame = 2 
Query:  332 MKSEYAEFFEISNPLASSLSIGKKEPNHLWNERNSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS 1081
            MKSEYAEFFEI+NPLASSLSIGKKEPNH+WNERNSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPS+NNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDN LMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS
Sbjct:    1 MKSEYAEFFEIANPLASSLSIGKKEPNHIWNERNSSYMTDTENPIGSEKITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSSNNTLTSICQSNPGFNNCEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNTLMSSHNKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS 250
[Close]
BLAST of Twist vs. Planmine SMEST
Match: SMESG000079648.1 (SMESG000079648.1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.057e-14
Identity = 41/87 (47.13%), Postives = 55/87 (63.22%), Query Frame = 2 
Query:  482 TCSPNVG------SSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQIL 721
            T SP++G      SS  R R   +  ++L  QR  AN+RER+R QS+N AF  LR  IPTLP +K LSK+ TL+LA  YI+FL  ++
Sbjct:   72 TTSPDIGKLGSGFSSKYRNRNKTRRGQKLVFQRQAANLRERRRMQSINEAFEGLRAHIPTLPYEKRLSKVDTLRLAIGYINFLQDLV 158
[Close]
BLAST of Twist vs. Planmine SMEST
Match: SMESG000044960.1 (SMESG000044960.1)

HSP 1 Score: 67.3958 bits (163), Expect = 6.482e-13
Identity = 33/62 (53.23%), Postives = 45/62 (72.58%), Query Frame = 2 
Query:  542 EELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILH 724
            E+L  QR  AN+RER+R QS+N AF +LR  IPT+P +K LSK+ TL++A  YI+FL  I+ 
Sbjct:   42 EKLARQRQAANMRERKRMQSINDAFEKLRVHIPTMPYEKRLSKVDTLRMAIGYINFLGGIMQ 103
[Close]
BLAST of Twist vs. Planmine SMEST
Match: SMESG000018258.1 (SMESG000018258.1)

HSP 1 Score: 63.929 bits (154), Expect = 9.756e-12
Identity = 34/82 (41.46%), Postives = 47/82 (57.32%), Query Frame = 2 
Query:  479 ITCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSD-KLSKIQTLKLAASYIDFLSQIL 721
            + C P + + N +RR           QR  AN RER R + L+ AF +L+  +P +P D KLSK+ TLKLAA YI +L  +L
Sbjct:   14 VECPPPLLTVNGKRRGRKPGANSTLAQRSAANSRERSRMRVLSSAFIDLKSALPWVPKDTKLSKLDTLKLAAGYIAYLKSVL 95
[Close]
BLAST of Twist vs. Planmine SMEST
Match: SMESG000029000.1 (SMESG000029000.1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.123e-11
Identity = 36/80 (45.00%), Postives = 53/80 (66.25%), Query Frame = 2 
Query:  488 SPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRRIIPTLPSDK-LSKIQTLKLAASYIDFLSQILH 724
            SPN  +  KR+R S  +      QR  AN+RER+R  SLN AF  LR+ +PT   +K LS+I+TL+LA +YI F++++L+
Sbjct:  128 SPNPSNKPKRKRISTVA------QRRAANIRERRRMYSLNEAFDSLRKRVPTFAYEKRLSRIETLRLAITYICFMTELLN 201
The following BLAST results are available for this feature:
BLAST of Twist vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TWIST22.379e-3352.42twist family bHLH transcription factor 2 [Source:H... [more]
TWIST22.379e-3352.42twist family bHLH transcription factor 2 [Source:H... [more]
TWIST22.379e-3352.42twist family bHLH transcription factor 2 [Source:H... [more]
TWIST22.379e-3352.42twist family bHLH transcription factor 2 [Source:H... [more]
TWIST16.466e-3053.70twist family bHLH transcription factor 1 [Source:H... [more]
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BLAST of Twist vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hlh-86.044e-1552.11Twist-related protein [Source:UniProtKB/Swiss-Pro... [more]
lin-327.003e-1143.37pep chromosome:WBcel235:X:2229208:2230122:-1 gene:... [more]
hlh-103.027e-1038.10Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q23... [more]
hlh-133.102e-1050.79Helix-loop-helix protein 13 [Source:UniProtKB/Swi... [more]
hlh-151.682e-952.00Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18... [more]
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BLAST of Twist vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
twi1.326e-2163.64gene:FBgn0003900 transcript:FBtr0071953[more]
twi1.326e-2163.64gene:FBgn0003900 transcript:FBtr0345653[more]
twi1.326e-2163.64gene:FBgn0003900 transcript:FBtr0100130[more]
Fer12.717e-1546.15gene:FBgn0037475 transcript:FBtr0334670[more]
Fer11.742e-1351.90gene:FBgn0037475 transcript:FBtr0081567[more]
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BLAST of Twist vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
twist21.196e-3052.07twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235][more]
twist1b9.014e-3081.25twist family bHLH transcription factor 1b [Source:... [more]
twist1b1.323e-2952.78twist family bHLH transcription factor 1b [Source:... [more]
twist1a2.773e-2770.83twist family bHLH transcription factor 1a [Source:... [more]
twist1a2.773e-2770.83twist family bHLH transcription factor 1a [Source:... [more]
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BLAST of Twist vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rab341.295e-2750.94RAB34, member RAS oncogene family [Source:Xenbase;... [more]
csnk1a17.977e-2673.44casein kinase 1 alpha 1 [Source:Xenbase;Acc:XB-GEN... [more]
adra1d2.325e-1754.65adrenoceptor alpha 1D [Source:Xenbase;Acc:XB-GENE-... [more]
PTF1A1.363e-1448.31pancreas associated transcription factor 1a [Sourc... [more]
tcf156.113e-1339.84transcription factor 15 [Source:NCBI gene;Acc:5492... [more]
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BLAST of Twist vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Twist21.634e-3352.42twist basic helix-loop-helix transcription factor ... [more]
Twist21.634e-3352.42twist basic helix-loop-helix transcription factor ... [more]
Twist11.924e-2953.70twist basic helix-loop-helix transcription factor ... [more]
Scx1.680e-1351.35scleraxis [Source:MGI Symbol;Acc:MGI:102934][more]
Tcf157.137e-1350.67transcription factor 15 [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of Twist vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9D030|TWST2_MOUSE7.432e-3352.42Twist-related protein 2 OS=Mus musculus OX=10090 G... [more]
sp|P97831|TWST2_RAT1.143e-3252.42Twist-related protein 2 OS=Rattus norvegicus OX=10... [more]
sp|Q8WVJ9|TWST2_HUMAN1.143e-3252.42Twist-related protein 2 OS=Homo sapiens OX=9606 GN... [more]
sp|O96642|TWIST_BRABE2.229e-3058.25Twist-related protein OS=Branchiostoma belcheri OX... [more]
sp|P13903|TWIST_XENLA2.612e-2946.32Twist-related protein OS=Xenopus laevis OX=8355 GN... [more]
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BLAST of Twist vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
D3J1K51.575e-17395.20Twist OS=Schmidtea polychroa OX=50054 GN=twi PE=2 ... [more]
U5YR671.306e-15595.98Twist protein (Fragment) OS=Schmidtea mediterranea... [more]
A0A091CN749.667e-3642.11Twist-related protein 2 OS=Fukomys damarensis OX=8... [more]
A0A2P8XME52.202e-3345.99Twist-related protein 2 OS=Blattella germanica OX=... [more]
A0A4Y7LMB16.001e-3246.37EOG090X0511 OS=Daphnia similis OX=35528 GN=EOG090X... [more]
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BLAST of Twist vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
twist1a3.215e-3141.48twist family bHLH transcription factor 1a [Source:... [more]
twist27.290e-3166.29twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235][more]
ENSAMXT00000026383.21.154e-2660.00pep primary_assembly:Astyanax_mexicanus-2.0:13:850... [more]
twist31.127e-2557.30twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7][more]
ENSAMXT00000025720.22.810e-2445.61twist-related protein-like [Source:NCBI gene;Acc:1... [more]
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BLAST of Twist vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TWIST11.195e-3154.13twist family bHLH transcription factor 1 [Source:H... [more]
TWIST13.641e-2867.09twist family bHLH transcription factor 1 [Source:H... [more]
TWIST11.020e-2767.09twist family bHLH transcription factor 1 [Source:H... [more]
ENSPMAT00000011442.12.476e-2183.67pep scaffold:Pmarinus_7.0:GL478554:30221:30385:-1 ... [more]
scxa1.902e-1260.71scleraxis bHLH transcription factor a [Source:ZFIN... [more]
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BLAST of Twist vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Twist vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
TWIST5.023e-2470.77Predicted protein; Twist family bHLH transcription... [more]
EDO438493.310e-1457.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429245.759e-1466.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO408123.457e-1164.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO461932.473e-1051.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Twist vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
twist22.590e-3174.03twist2 [Source:ZFIN;Acc:ZDB-GENE-980526-235][more]
twist18.101e-3052.78twist family bHLH transcription factor 1 [Source:N... [more]
ENSORLT00000005014.22.331e-2765.79twist family bHLH transcription factor 1 [Source:N... [more]
twist33.010e-2562.82twist-related protein 2-like [Source:NCBI gene;Acc... [more]
ENSORLT00000026838.11.535e-2362.16twist-related protein 2-like [Source:NCBI gene;Acc... [more]
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BLAST of Twist vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026385.10.000e+098.40SMESG000026385.1[more]
SMESG000079648.17.057e-1447.13SMESG000079648.1[more]
SMESG000044960.16.482e-1353.23SMESG000044960.1[more]
SMESG000018258.19.756e-1241.46SMESG000018258.1[more]
SMESG000029000.11.123e-1145.00SMESG000029000.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008927 ID=SMED30008927|Name=Twist|organism=Schmidtea mediterranea sexual|type=transcript|length=1137bp
CGCGCAAAAATCAAATTGTGCTTCTACTCTTGCGCACAAATCCCATTTAC
ATTGACCAACAAAATATCACGAACAACCCAGTTTATACATTTGACAAACA
CACATACACACACACGCAAAAAACATTCGCACAAAAGCGAATATTTCGCT
ACACACAGATTGGGTTTTGTCCAATGACACATGGTTTTCTAAAAAACGAT
GTTGTTTGCACCAGCCGCAAGGCAGGCGAATATTCGTAAATGTGTAACTG
TTGTGACAAGAAAGCGAACATCTGGAATGTGATAATGAGCCTGATTTAAA
TTGAGTTTTTATTTTTGAAAATAAAAAAAACATGAAATCAGAATATGCAG
AATTTTTTGAGATTTCTAATCCACTCGCGAGTAGTCTTTCAATCGGCAAG
AAAGAACCGAACCATCTTTGGAATGAAAGAAATTCATCATATATGACTGA
TACAGAAAACCCAATCGGGTCGGAAAAAATTACATGTTCACCAAATGTTG
GAAGTTCAAACAAACGCAGAAGAAAATCAGCACAATCGTACGAAGAGTTA
CAAAACCAAAGATTTCTAGCTAATGTACGAGAACGACAAAGAACTCAGTC
ATTAAATCGAGCTTTCAGTGAGTTGAGAAGAATCATTCCAACGTTACCCT
CTGATAAGCTAAGTAAGATACAAACGTTAAAATTAGCGGCGAGTTATATT
GACTTTTTAAGTCAAATTTTGCATCCGTCCGCCAACAATACTTTAACAAG
CATCTGCCAGTCGAATCCAGGATTCAATAATTGTGAATATAATTCTTACA
ATAACATACCATTCTCCAATGATCTATATACCATTGCCAATCATCAGACC
ACTTCAAAAGTCAACAATCCCCAGATTTTAAAATTCCTATCTAACAATGC
CAACGTTGACAATGCTCTAATGTCGAGTCACAATAAAAATGTCCTATTAC
ATTCTCTCGATCAAAATCTTTATGGAAATACAAGTACACCTCATTCCTCA
GACGTTTCTAATAACGCTCACGAAACTCTTAGTTACGCATTCTCAGTTTG
GCGAATGGAAGGAGCTTGGGCATCTCCGTCATGACTATAAAATTAATATC
TACTTGTATATAATTGAAAAGTATGAGATTAATTTCT
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protein sequence of SMED30008927-orf-1

>SMED30008927-orf-1 ID=SMED30008927-orf-1|Name=SMED30008927-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp
MKSEYAEFFEISNPLASSLSIGKKEPNHLWNERNSSYMTDTENPIGSEKI
TCSPNVGSSNKRRRKSAQSYEELQNQRFLANVRERQRTQSLNRAFSELRR
IIPTLPSDKLSKIQTLKLAASYIDFLSQILHPSANNTLTSICQSNPGFNN
CEYNSYNNIPFSNDLYTIANHQTTSKVNNPQILKFLSNNANVDNALMSSH
NKNVLLHSLDQNLYGNTSTPHSSDVSNNAHETLSYAFSVWRMEGAWASPS
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Aliases
The feature 'Twist' has the following synonyms
Synonym
twist
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000420parapharyngeal region
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR015789Twist-related
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 81..132
e-value: 1.2E-16
score: 71.3
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 80..126
e-value: 1.2E-14
score: 54.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 75..126
score: 15.917
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 75..131
e-value: 1.33713E-14
score: 64.5427
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 58..131
e-value: 1.7E-21
score: 77.6
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 75..133
NoneNo IPR availablePANTHERPTHR23349BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWISTcoord: 33..248
IPR015789Twist-relatedPANTHERPTHR23349:SF50PROTEIN TWISTcoord: 33..248