CDGSH iron-sulfur domain-containing protein 3 mitochondrial

Overview
NameCDGSH iron-sulfur domain-containing protein 3 mitochondrial
Smed IDSMED30008922
Length (bp)581
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CDGSH iron-sulfur domain-containing protein 3 mitochondrial (SMED30008922) t-SNE clustered cells

Violin plots show distribution of expression levels for CDGSH iron-sulfur domain-containing protein 3 mitochondrial (SMED30008922) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CDGSH iron-sulfur domain-containing protein 3 mitochondrial (SMED30008922) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CDGSH iron-sulfur domain-containing protein 3 mitochondrial (SMED30008922) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008922

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008922h1SMcG0014820 SmedASXL_061241SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Human
Match: CISD3 (CDGSH iron sulfur domain 3 [Source:HGNC Symbol;Acc:HGNC:27578])

HSP 1 Score: 79.7221 bits (195), Expect = 7.117e-19
Identity = 38/91 (41.76%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:  263 VYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            V    P KV+   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++   V  C CK T  PP+CDG+H SE V+K+
Sbjct:   41 VALKTPIKVELVAGKTYRWCVCGRSKKQPFCDGSH----------FFQRTGLSPLKFKAQETRMVALCTCKATQRPPYCDGTHRSERVQKA 121
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Human
Match: CISD3 (CDGSH iron sulfur domain 3 [Source:HGNC Symbol;Acc:HGNC:27578])

HSP 1 Score: 79.7221 bits (195), Expect = 7.117e-19
Identity = 38/91 (41.76%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:  263 VYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            V    P KV+   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++   V  C CK T  PP+CDG+H SE V+K+
Sbjct:   41 VALKTPIKVELVAGKTYRWCVCGRSKKQPFCDGSH----------FFQRTGLSPLKFKAQETRMVALCTCKATQRPPYCDGTHRSERVQKA 121
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Celegans
Match: cisd-3.1 (CDGSH Iron Sulfur Domain protein homolog [Source:UniProtKB/TrEMBL;Acc:Q9U3A1])

HSP 1 Score: 91.2781 bits (225), Expect = 2.286e-23
Identity = 47/95 (49.47%), Postives = 59/95 (62.11%), Query Frame = 2 
Query:  257 GKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHS--SEEVKKS 535
            GKV+   P KV  K  KVY WCSCG S +QP CDG+H       +S  +   K  P++ + +K  TVW CNCKQTNN PFCDGSH   ++E KK+
Sbjct:   69 GKVHSKLPTKVHMKKDKVYAWCSCGYSGSQPLCDGSH-------NSIRIPDLKLKPVRFIPDKDMTVWLCNCKQTNNRPFCDGSHKTVTDEDKKA 156
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Celegans
Match: cisd-3.2 (CDGSH Iron Sulfur Domain protein homolog [Source:UniProtKB/TrEMBL;Acc:Q9BKQ9])

HSP 1 Score: 65.855 bits (159), Expect = 1.200e-13
Identity = 36/87 (41.38%), Postives = 44/87 (50.57%), Query Frame = 2 
Query:  254 KGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHS 514
            KG+    K  +V  + GK Y WCSCG S TQPFCDG H+   G T+          P+    EK      C+CKQT   P CDG H+
Sbjct:   39 KGQPAGTKSNRVKLEAGKTYHWCSCGLSITQPFCDGTHKTP-GLTN--------VRPVSFQVEKTGIYPMCDCKQTGTRPICDGKHA 116
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Fly
Match: CG3420 (gene:FBgn0033100 transcript:FBtr0332940)

HSP 1 Score: 83.5741 bits (205), Expect = 1.587e-20
Identity = 38/102 (37.25%), Postives = 55/102 (53.92%), Query Frame = 2 
Query:  230 QYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            Q  +     G ++D +PFK+     K Y WC CG SK+QP CDG H+      + +   +Q+  PI+   EK    W CNCKQT + PFCDG+H    ++ +
Sbjct:   41 QTAVLQKENGTIFDKRPFKIHLDKDKTYSWCLCGKSKSQPLCDGMHK------NEFLKIKQR--PIRFKVEKSGDYWLCNCKQTTHRPFCDGTHKQPHIQSA 134
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Fly
Match: CG3420 (gene:FBgn0033100 transcript:FBtr0086135)

HSP 1 Score: 83.5741 bits (205), Expect = 1.587e-20
Identity = 38/102 (37.25%), Postives = 55/102 (53.92%), Query Frame = 2 
Query:  230 QYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            Q  +     G ++D +PFK+     K Y WC CG SK+QP CDG H+      + +   +Q+  PI+   EK    W CNCKQT + PFCDG+H    ++ +
Sbjct:   41 QTAVLQKENGTIFDKRPFKIHLDKDKTYSWCLCGKSKSQPLCDGMHK------NEFLKIKQR--PIRFKVEKSGDYWLCNCKQTTHRPFCDGTHKQPHIQSA 134
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Xenopus
Match: lyrm2 (LYR motif containing 2 [Source:Xenbase;Acc:XB-GENE-5753220])

HSP 1 Score: 85.5001 bits (210), Expect = 1.935e-21
Identity = 44/104 (42.31%), Postives = 55/104 (52.88%), Query Frame = 2 
Query:  224 RNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            R Q   T   K  +    PFKV+ K GK+Y WCSCG SK QPFCDG+HR      S          P++    +   VW C CKQT NPP+CDG+H    ++ S
Sbjct:   12 RFQACSTSQPKSVIAAKHPFKVELKAGKMYPWCSCGHSKKQPFCDGSHRQAAPGLS----------PLRFTPTEDQEVWLCGCKQTKNPPYCDGTHKETHIQNS 105
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Xenopus
Match: lyrm2 (LYR motif containing 2 [Source:Xenbase;Acc:XB-GENE-5753220])

HSP 1 Score: 85.5001 bits (210), Expect = 2.558e-21
Identity = 42/98 (42.86%), Postives = 53/98 (54.08%), Query Frame = 2 
Query:  242 TPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            T   K  +    PFKV+ K GK+Y WCSCG SK QPFCDG+HR      S          P++    +   VW C CKQT NPP+CDG+H    ++ S
Sbjct:   18 TSQPKSVIAAKHPFKVELKAGKMYPWCSCGHSKKQPFCDGSHRQAAPGLS----------PLRFTPTEDQEVWLCGCKQTKNPPYCDGTHKETHIQNS 105
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Mouse
Match: Cisd3 (CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:MGI:101788])

HSP 1 Score: 79.337 bits (194), Expect = 6.047e-19
Identity = 37/91 (40.66%), Postives = 51/91 (56.04%), Query Frame = 2 
Query:  263 VYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            V    P +++   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++  TV  C CK T  PP+CDG+H SE+V+K+
Sbjct:   29 VAQKTPIRLELVAGKTYRWCVCGRSKNQPFCDGSH----------FFQRTGLSPLKFKAQETRTVALCTCKATQRPPYCDGTHKSEQVQKA 109
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Mouse
Match: Cisd3 (CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:MGI:101788])

HSP 1 Score: 79.7221 bits (195), Expect = 6.152e-19
Identity = 37/91 (40.66%), Postives = 51/91 (56.04%), Query Frame = 2 
Query:  263 VYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            V    P +++   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++  TV  C CK T  PP+CDG+H SE+V+K+
Sbjct:   48 VAQKTPIRLELVAGKTYRWCVCGRSKNQPFCDGSH----------FFQRTGLSPLKFKAQETRTVALCTCKATQRPPYCDGTHKSEQVQKA 128
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Mouse
Match: Cisd3 (CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:MGI:101788])

HSP 1 Score: 79.337 bits (194), Expect = 8.744e-19
Identity = 38/95 (40.00%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:  251 VKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
             K  V    P +++   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++  TV  C CK T  PP+CDG+H SE+V+K+
Sbjct:   47 AKPVVAQKTPIRLELVAGKTYRWCVCGRSKNQPFCDGSH----------FFQRTGLSPLKFKAQETRTVALCTCKATQRPPYCDGTHKSEQVQKA 131
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. UniProt/SwissProt
Match: sp|P0C7P0|CISD3_HUMAN (CDGSH iron-sulfur domain-containing protein 3, mitochondrial OS=Homo sapiens OX=9606 GN=CISD3 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.418e-18
Identity = 38/91 (41.76%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:  263 VYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            V    P KV+   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++   V  C CK T  PP+CDG+H SE V+K+
Sbjct:   41 VALKTPIKVELVAGKTYRWCVCGRSKKQPFCDGSH----------FFQRTGLSPLKFKAQETRMVALCTCKATQRPPYCDGTHRSERVQKA 121
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. UniProt/SwissProt
Match: sp|B1AR13|CISD3_MOUSE (CDGSH iron-sulfur domain-containing protein 3, mitochondrial OS=Mus musculus OX=10090 GN=Cisd3 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.116e-18
Identity = 38/95 (40.00%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:  251 VKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
             K  V    P +++   GK Y WC CG SK QPFCDG+H          F  +   +P+K   ++  TV  C CK T  PP+CDG+H SE+V+K+
Sbjct:   47 AKPVVAQKTPIRLELVAGKTYRWCVCGRSKNQPFCDGSH----------FFQRTGLSPLKFKAQETRTVALCTCKATQRPPYCDGTHKSEQVQKA 131
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Match: A0A430PXR8 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0005819 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 9.936e-45
Identity = 70/135 (51.85%), Postives = 94/135 (69.63%), Query Frame = 2 
Query:  125 KIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVK 529
            + FP   LY P  E +DEN ++KP  HPETPRF+      P  K K+   +P  V C+P +VYWWCSCG SK QPFCDG+H  ILGS  ++ + +++F PI+ V+E+P+ VWFC CKQT+ PP+CDGSH+ EEV+
Sbjct:   26 RYFPPGTLYRPHHERFDENCERKPLPHPETPRFQEIPVTDPRSKAKIAGKRPIHVICEPNQVYWWCSCGHSKRQPFCDGHHIRILGSNPTFKINKREFKPIRLVYEQPSEVWFCTCKQTSEPPYCDGSHNCEEVQ 160
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Match: A0A430QQG9 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0008594 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 1.108e-44
Identity = 70/135 (51.85%), Postives = 94/135 (69.63%), Query Frame = 2 
Query:  125 KIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVK 529
            + FP   LY P  E +DEN ++KP  HPETPRF+      P  K K+   +P  V C+P +VYWWCSCG SK QPFCDG+H  ILGS  ++ + +++F PI+ V+E+P+ VWFC CKQT+ PP+CDGSH+ EEV+
Sbjct:   26 RYFPPGTLYRPHHERFDENCERKPLPHPETPRFQEIPVTDPRSKAKIAGKRPIHVICEPNQVYWWCSCGHSKRQPFCDGHHIRILGSNPTFKINKREFKPIRLVYEQPSEVWFCTCKQTSEPPYCDGSHNCEEVQ 160
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Match: A0A3Q0KQQ5 (Putative phosphoglucomutase OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 5.257e-44
Identity = 70/135 (51.85%), Postives = 94/135 (69.63%), Query Frame = 2 
Query:  125 KIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVK 529
            + FP   LY P +E +DEN ++KP  HPETPRF+      P  K K+   +P  V C+P +VYWWCSCG SK QPFCDG+H  ILGS   + + +++F PI+ V+E+P+ VWFC CKQT+ PP+CDGSH+ EEV+
Sbjct:   26 RYFPPGTLYRPHTERFDENCERKPLPHPETPRFQELPVTDPRNKPKIAGKRPIHVICEPNQVYWWCSCGHSKRQPFCDGHHIRILGSNPGFKIHKREFKPIRLVYEQPSEVWFCTCKQTSEPPYCDGSHNCEEVQ 160
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Match: A0A183NXY9 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS6974 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 5.798e-44
Identity = 69/135 (51.11%), Postives = 93/135 (68.89%), Query Frame = 2 
Query:  125 KIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVK 529
            + FP   LY P  E +DEN + KP  HPETPRF+      P  K K+   +P  V C+P +VYWWCSCG SK QPFCDG+H  ILGS  ++ + +++F P++ V+E+P+ VWFC CKQT+ PP+CDGSH+ EEV+
Sbjct:   26 RYFPPGTLYRPHHERFDENCEHKPLPHPETPRFQEIPVTDPRSKAKIAGKRPIHVICEPNQVYWWCSCGHSKRQPFCDGHHIRILGSNPTFKINKREFKPVRLVYEQPSEVWFCTCKQTSEPPYCDGSHNCEEVQ 160
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Match: A0A5J4NVK9 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0003851 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 6.270e-44
Identity = 70/157 (44.59%), Postives = 101/157 (64.33%), Query Frame = 2 
Query:   68 ALTKKLNCIDVNLTIKRCK-KIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            AL   L+   V L + RC  +  P   L++P  E +DEN + +P LHPETPRF++      + KG++ D +P +V C P +VYWWCSCG S +QPFCDG H  I+G T  + + + +F P++  F++ T VW+C CKQTN PP+CDGSH+  EV+ +
Sbjct:    6 ALGSSLSIYRV-LHVPRCSGRYMPPGCLFLPHHERFDENCEPRPPLHPETPRFKDALNTGAAPKGRIADKRPHRVVCLPNRVYWWCSCGHSNSQPFCDGQHIRIIGPTPHFHINKVQFKPVRMTFKEKTEVWWCTCKQTNEPPYCDGSHNCSEVQSA 161
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002612.1 (pep scaffold:Pmarinus_7.0:GL477054:222771:227854:1 gene:ENSPMAG00000002381.1 transcript:ENSPMAT00000002612.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 2.703e-17
Identity = 35/79 (44.30%), Postives = 46/79 (58.23%), Query Frame = 2 
Query:  275 KPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSH 511
            KPF+++ KPGK + WC+CG S  QPFCDG+H        + +  + K  P+     +    W C CKQT  PPFCDGSH
Sbjct:   67 KPFRLEGKPGKRHLWCACGFSGNQPFCDGSH------FKARWKGESKKWPVLFKVPESGIAWLCLCKQTGKPPFCDGSH 139
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Nematostella
Match: EDO48925 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHW5])

HSP 1 Score: 69.707 bits (169), Expect = 3.405e-16
Identity = 36/86 (41.86%), Postives = 52/86 (60.47%), Query Frame = 2 
Query:  278 PFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            P++V+ K  K Y WC CG SK QPFCDG+H+   GS     +  ++F P +   + P   + C CKQT  PP+CDG+H+S  V+ +
Sbjct:    1 PYEVELKADKRYSWCRCGLSKKQPFCDGSHK---GSG----IAPERFRPTQ---DNP-LAFLCGCKQTGTPPYCDGTHASPMVEMA 75
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Nematostella
Match: EDO44292 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWC5])

HSP 1 Score: 58.9214 bits (141), Expect = 1.506e-11
Identity = 34/81 (41.98%), Postives = 47/81 (58.02%), Query Frame = 2 
Query:  278 PFKVD-CKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIK-TVFEKPTTVW-FCNCKQTNNPPFCDGSH 511
            P+ V+  +PGK+  WC+CG SK QP+CDG H+               F  +K  V +KP  ++  C CK T++PPFCDGSH
Sbjct:   30 PYSVENLEPGKIRKWCTCGLSKKQPWCDGTHKGT------------GFKSLKWKVPDKPQKLYSICGCKHTSSPPFCDGSH 98
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Medaka
Match: ENSORLT00000040418.1 (CDGSH iron sulfur domain 3 [Source:NCBI gene;Acc:101168272])

HSP 1 Score: 69.707 bits (169), Expect = 3.229e-15
Identity = 34/78 (43.59%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:  278 PFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSH 511
            P +V    GK Y WC+CG SK QPFCDG H                 +P++   +K  TV+ C CKQT N P+CDGSH
Sbjct:   48 PCRVKLSAGKRYAWCACGYSKKQPFCDGAH----------LKQAPSISPLRFSPDKDGTVFLCACKQTKNAPYCDGSH 115
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Planmine SMEST
Match: SMESG000012454.1 (SMESG000012454.1)

HSP 1 Score: 327.02 bits (837), Expect = 3.900e-116
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 2 
Query:   62 MNALTKKLNCIDVNLTIKRCKKIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 535
            MNALTKKLNCIDVNLTIKRCKKIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS
Sbjct:    1 MNALTKKLNCIDVNLTIKRCKKIFPICHLYIPKSELYDENGDKKPRLHPETPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSHSSEEVKKS 158
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Planmine SMEST
Match: SMESG000016828.1 (SMESG000016828.1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.736e-12
Identity = 33/76 (43.42%), Postives = 44/76 (57.89%), Query Frame = 2 
Query:  290 DCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVW-FCNCKQTNNPPFCDGSHS 514
            D KPG   +WC+CG SK QP+CDG+H+   G T   F+  +    +K      T V+  C CK + N PFCDG+HS
Sbjct:   19 DLKPGDKKYWCACGLSKNQPWCDGSHKDT-GITPLEFIVPE----LKPGESAETKVYSMCQCKHSKNKPFCDGTHS 89
[Close]
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Planmine SMEST
Match: SMESG000016828.1 (SMESG000016828.1)

HSP 1 Score: 60.8474 bits (146), Expect = 6.811e-12
Identity = 33/76 (43.42%), Postives = 44/76 (57.89%), Query Frame = 2 
Query:  290 DCKPGKVYWWCSCGTSKTQPFCDGNHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVW-FCNCKQTNNPPFCDGSHS 514
            D KPG   +WC+CG SK QP+CDG+H+   G T   F+  +    +K      T V+  C CK + N PFCDG+HS
Sbjct:   45 DLKPGDKKYWCACGLSKNQPWCDGSHKDT-GITPLEFIVPE----LKPGESAETKVYSMCQCKHSKNKPFCDGTHS 115
The following BLAST results are available for this feature:
BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CISD37.117e-1941.76CDGSH iron sulfur domain 3 [Source:HGNC Symbol;Acc... [more]
CISD37.117e-1941.76CDGSH iron sulfur domain 3 [Source:HGNC Symbol;Acc... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cisd-3.12.286e-2349.47CDGSH Iron Sulfur Domain protein homolog [Source:... [more]
cisd-3.21.200e-1341.38CDGSH Iron Sulfur Domain protein homolog [Source:... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG34201.587e-2037.25gene:FBgn0033100 transcript:FBtr0332940[more]
CG34201.587e-2037.25gene:FBgn0033100 transcript:FBtr0086135[more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lyrm21.935e-2142.31LYR motif containing 2 [Source:Xenbase;Acc:XB-GENE... [more]
lyrm22.558e-2142.86LYR motif containing 2 [Source:Xenbase;Acc:XB-GENE... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Cisd36.047e-1940.66CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:... [more]
Cisd36.152e-1940.66CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:... [more]
Cisd38.744e-1940.00CDGSH iron sulfur domain 3 [Source:MGI Symbol;Acc:... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|P0C7P0|CISD3_HUMAN3.418e-1841.76CDGSH iron-sulfur domain-containing protein 3, mit... [more]
sp|B1AR13|CISD3_MOUSE6.116e-1840.00CDGSH iron-sulfur domain-containing protein 3, mit... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A430PXR89.936e-4551.85Uncharacterized protein OS=Schistosoma bovis OX=61... [more]
A0A430QQG91.108e-4451.85Uncharacterized protein OS=Schistosoma bovis OX=61... [more]
A0A3Q0KQQ55.257e-4451.85Putative phosphoglucomutase OS=Schistosoma mansoni... [more]
A0A183NXY95.798e-4451.11Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A5J4NVK96.270e-4444.59Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000002612.12.703e-1744.30pep scaffold:Pmarinus_7.0:GL477054:222771:227854:1... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO489253.405e-1641.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442921.506e-1141.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000040418.13.229e-1543.59CDGSH iron sulfur domain 3 [Source:NCBI gene;Acc:1... [more]
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BLAST of CDGSH iron-sulfur domain-containing protein 3 mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000012454.13.900e-116100.00SMESG000012454.1[more]
SMESG000016828.14.736e-1243.42SMESG000016828.1[more]
SMESG000016828.16.811e-1243.42SMESG000016828.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008922 ID=SMED30008922|Name=CDGSH iron-sulfur domain-containing protein 3 mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=581bp
TAAAAATATATATTTTTTTAAATATAAATTATTAATTTAGGATTTATTCA
AATTAAAAATAATGAATGCGCTTACTAAAAAGCTTAATTGTATAGATGTA
AATTTAACAATTAAACGATGTAAAAAAATTTTTCCTATCTGTCATTTATA
TATTCCTAAGTCAGAATTATATGATGAAAATGGCGATAAAAAGCCTAGAT
TGCATCCAGAAACTCCTAGATTTAGAAATCAGTACGGTATAACACCTAGT
GTAAAAGGAAAAGTATATGATAATAAACCGTTTAAAGTGGATTGTAAGCC
TGGCAAAGTGTACTGGTGGTGTTCGTGTGGTACAAGTAAAACTCAGCCGT
TTTGTGATGGTAATCACAGAATAATTCTGGGCTCTACTTCTTCGTATTTT
GTTACACAACAAAAATTTAATCCTATAAAAACAGTTTTCGAAAAACCGAC
AACTGTTTGGTTTTGTAATTGCAAGCAAACAAATAATCCACCATTTTGTG
ATGGGTCTCATTCGTCGGAAGAAGTCAAAAAATCATGAAATTCCATATTT
TAAATTAAATACCTTCATAAATGAAAAAAAA
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protein sequence of SMED30008922-orf-1

>SMED30008922-orf-1 ID=SMED30008922-orf-1|Name=SMED30008922-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MNALTKKLNCIDVNLTIKRCKKIFPICHLYIPKSELYDENGDKKPRLHPE
TPRFRNQYGITPSVKGKVYDNKPFKVDCKPGKVYWWCSCGTSKTQPFCDG
NHRIILGSTSSYFVTQQKFNPIKTVFEKPTTVWFCNCKQTNNPPFCDGSH
SSEEVKKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR018967FeS-contain_CDGSH-typ
Vocabulary: cellular component
TermDefinition
GO:0043231intracellular membrane-bounded organelle
Vocabulary: molecular function
TermDefinition
GO:00515372 iron, 2 sulfur cluster binding