Kinesin-associated protein 3

Overview
NameKinesin-associated protein 3
Smed IDSMED30008904
Length (bp)2635
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Kinesin-associated protein 3 (SMED30008904) t-SNE clustered cells

Violin plots show distribution of expression levels for Kinesin-associated protein 3 (SMED30008904) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kinesin-associated protein 3 (SMED30008904) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kinesin-associated protein 3 (SMED30008904) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30008904

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30008904h1SMcG0018090 dd_Smed_v4_4094_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30008904h1SMcG0018090 dd_Smed_v4_4094_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008904h1SMcG0018090 dd_Smed_v6_4094_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
oocyteSMED30008904h1SMcG0018090 Contig29497newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30008904h1SMcG0018090 Contig29497uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30008904h1SMcG0018090 Contig29497newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30008904h1SMcG0018090 Contig29497uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008904h1SMcG0018090 Contig29497newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008904h1SMcG0018090 Contig29497uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30008904h1SMcG0018090 dd_Smed_v4_4094_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Kinesin-associated protein 3 vs. Ensembl Human
Match: KIFAP3 (kinesin associated protein 3 [Source:HGNC Symbol;Acc:HGNC:17060])

HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0
Identity = 439/809 (54.26%), Postives = 589/809 (72.81%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            +D RYLK+ ++ G IDVHP+EKALIV+YE++ATI GE+G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I+N     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + ++D +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+PV +              RP    GFR D
Sbjct:    4 EDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQMDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLG--MDGFGQPVGILG------------RPATAYGFRPD 783          
BLAST of Kinesin-associated protein 3 vs. Ensembl Human
Match: KIFAP3 (kinesin associated protein 3 [Source:HGNC Symbol;Acc:HGNC:17060])

HSP 1 Score: 739.569 bits (1908), Expect = 0.000e+0
Identity = 415/772 (53.76%), Postives = 557/772 (72.15%), Query Frame = 3
Query:  195 LGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            +G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I+N     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + ++D +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+PV +              RP    GFR D
Sbjct:    1 MGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQMDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLG--MDGFGQPVGILG------------RPATAYGFRPD 743          
BLAST of Kinesin-associated protein 3 vs. Ensembl Human
Match: KIFAP3 (kinesin associated protein 3 [Source:HGNC Symbol;Acc:HGNC:17060])

HSP 1 Score: 716.457 bits (1848), Expect = 0.000e+0
Identity = 407/768 (52.99%), Postives = 547/768 (71.22%), Query Frame = 3
Query:  207 MAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            M G+     K IR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I+N     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + ++D +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+PV +              RP    GFR D
Sbjct:    1 MQGEDARYLKSIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQMDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLG--MDGFGQPVGILG------------RPATAYGFRPD 739          
BLAST of Kinesin-associated protein 3 vs. Ensembl Human
Match: KIFAP3 (kinesin associated protein 3 [Source:HGNC Symbol;Acc:HGNC:17060])

HSP 1 Score: 665.611 bits (1716), Expect = 0.000e+0
Identity = 375/696 (53.88%), Postives = 497/696 (71.41%), Query Frame = 3
Query:  423 DPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I+N     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + ++D +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+PV +              RP    GFR D
Sbjct:   39 DPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQMDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLG--MDGFGQPVGILG------------RPATAYGFRPD 705          
BLAST of Kinesin-associated protein 3 vs. Ensembl Celegans
Match: kap-1 (Kinesin associated protein kap-1; Kinesin-Associated Protein [Source:UniProtKB/TrEMBL;Acc:G5EEE6])

HSP 1 Score: 355.525 bits (911), Expect = 1.211e-110
Identity = 238/686 (34.69%), Postives = 376/686 (54.81%), Query Frame = 3
Query:  123 TIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLY-EDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLE---RKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVI--SEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSG---KFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDD----------LILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFH-ESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIE 2120
            +ID HP+++A+IV +E   T    LG+         QKII +K +  + +I  L+  I+  C  I  +   ++E +L Y+  R    +S     +S    D     S  I   A L   DEY+E  Y E   +K K +  + +L++NP NL +L +N TL+ ALARV REDWK+  ++ TNI+ +F   S FS  H I++H KIGTL +N ++HE K+Y+ WI E++K   E       TL+K++T  RKQ  L       L N+A DIS+E+KM  +NLV +L K L+        L    + FL KLSI+ ENK  + +   +E+L K  P  + +L    + LL N SFDS    ++V  GL+P + +L   L+++T  L ++Y +SC D  K     TD I  LMK +L     +V     A+ +N+  +   A ++    G+GL +LM+ +  SRD M++K++R IS HEG  +  FL +I  L  +  N+     +    F +EC+G ++ L ++   +  I+   NLV WMK  LQ    E +          L L++V+  GT++     A+++A   +I + + L+ + Q DDE V Q++YVF Q + H E + +++ + +   A++IDLMHD N  +R+VCD  L I+ E  +EW+ +I  E+F+W+N+QWLEM+E
Sbjct:    5 SIDAHPSDQAIIVRFEQSPTNVESLGH---------QKIIHLKEMSLDVDIRALSNVILQKCLFIPATSRSQLEQVLFYIQKRGNQRISARSRSSSAVSFDRRPIHSPTIS--AELGKIDEYIECFYGETSVEKNKGAVALYELSKNPQNLTQLVNNETLMMALARVFREDWKKHFEVGTNIMNLFVNISKFSCLHGILLHHKIGTLCVNAMEHETKRYDFWIAEMKKTDQE-------TLRKLKTAIRKQAMLLAACVTFLTNLATDISVELKMVRRNLVALLVKCLQMSSESTSSLTTATIKFLLKLSIFDENKIVMEQNGTIEKLLKLFPIQDPELRKAVIMLLFNFSFDSKNLPKMVNGGLVPHMASL---LDSDTKALNMMYLLSCNDDAKAMLAYTDAIKLLMKDVLSGTGSEVT---KAVLLNICLEKRNAQLVCGQRGQGLDLLMEMSINSRDLMLIKVVRAISSHEGATQNMFLKWIETLIGIAKNEGADNSESKSSFGLECMGTVAELKVA--PWAKIIQSENLVPWMKTQLQEGIDESEEVTVLRDIKPLQLQIVIACGTMARQLDAARLLAP--LIDTFVQLLQSCQIDDEFVVQLLYVFLQFLKHKELSARLMTQDSALGAHMIDLMHDANAVVREVCDNALLIMGEHSKEWAKRIAGERFKWHNAQWLEMVE 662          
BLAST of Kinesin-associated protein 3 vs. Ensembl Celegans
Match: kap-1 (Kinesin associated protein kap-1; Kinesin-Associated Protein [Source:UniProtKB/TrEMBL;Acc:G5EEE6])

HSP 1 Score: 338.191 bits (866), Expect = 2.365e-104
Identity = 231/686 (33.67%), Postives = 367/686 (53.50%), Query Frame = 3
Query:  123 TIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLY-EDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLE---RKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVI--SEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSG---KFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDD----------LILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFH-ESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIE 2120
            +ID HP+++A+IV +E   T    LG+         QKII +K +  + +I  L+  I+  C  I  +   ++E +L Y+  R    +S     +S    D     S  I   A L   DEY+E  Y E   +K K +  + +L++NP NL +L +N TL+ ALARV REDWK+  ++ TNI+ +F                 IGTL +N ++HE K+Y+ WI E++K   E       TL+K++T  RKQ  L       L N+A DIS+E+KM  +NLV +L K L+        L    + FL KLSI+ ENK  + +   +E+L K  P  + +L    + LL N SFDS    ++V  GL+P + +L   L+++T  L ++Y +SC D  K     TD I  LMK +L     +V     A+ +N+  +   A ++    G+GL +LM+ +  SRD M++K++R IS HEG  +  FL +I  L  +  N+     +    F +EC+G ++ L ++   +  I+   NLV WMK  LQ    E +          L L++V+  GT++     A+++A   +I + + L+ + Q DDE V Q++YVF Q + H E + +++ + +   A++IDLMHD N  +R+VCD  L I+ E  +EW+ +I  E+F+W+N+QWLEM+E
Sbjct:    5 SIDAHPSDQAIIVRFEQSPTNVESLGH---------QKIIHLKEMSLDVDIRALSNVILQKCLFIPATSRSQLEQVLFYIQKRGNQRISARSRSSSAVSFDRRPIHSPTIS--AELGKIDEYIECFYGETSVEKNKGAVALYELSKNPQNLTQLVNNETLMMALARVFREDWKKHFEVGTNIMNLF-----------------IGTLCVNAMEHETKRYDFWIAEMKKTDQE-------TLRKLKTAIRKQAMLLAACVTFLTNLATDISVELKMVRRNLVALLVKCLQMSSESTSSLTTATIKFLLKLSIFDENKIVMEQNGTIEKLLKLFPIQDPELRKAVIMLLFNFSFDSKNLPKMVNGGLVPHMASL---LDSDTKALNMMYLLSCNDDAKAMLAYTDAIKLLMKDVLSGTGSEVT---KAVLLNICLEKRNAQLVCGQRGQGLDLLMEMSINSRDLMLIKVVRAISSHEGATQNMFLKWIETLIGIAKNEGADNSESKSSFGLECMGTVAELKVAP--WAKIIQSENLVPWMKTQLQEGIDESEEVTVLRDIKPLQLQIVIACGTMARQLDAARLLAP--LIDTFVQLLQSCQIDDEFVVQLLYVFLQFLKHKELSARLMTQDSALGAHMIDLMHDANAVVREVCDNALLIMGEHSKEWAKRIAGERFKWHNAQWLEMVE 645          
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Match: Kap3 (gene:FBgn0028421 transcript:FBtr0289954)

HSP 1 Score: 385.185 bits (988), Expect = 3.342e-119
Identity = 195/436 (44.72%), Postives = 308/436 (70.64%), Query Frame = 3
Query:  816 QQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG 2123
            +++++D+  K+++   +KQEQL RV++Y+LLN+AE++ LE KMR K++V+ML K L+R+N +LL+L+ +FLKKLSI G+NK ++   N+V +L +   +++ DL+ ++L+L+ N+SFD  LR +++  G LP LV      ++  + + ILY++S +DK K  F +T+C+      I+     KV ++++AL INL  +   A ++ EG+ L  LM RA+K +D ++MK+LRN+SQHE  ++++F+DY+ DLA ++T      D  F+VEC+GIL NL ++DLD+  IL  F L+ W++Q L P    DDL+L+ ++ +GT + D  CA +   A ++ SLI+L+ AKQEDDEIV QI++VF Q++ HESTR+ +I++T++ AY+IDLMHDKN EIRKVCD+ LDII+  D EW+ +I+ EKFR +NSQWL M+E 
Sbjct:  398 KKEEIDRLNKQLKIFAKKQEQLLRVAFYLLLNMAENVKLEEKMRRKHIVKMLVKALDRQNIDLLMLVSTFLKKLSIVGDNKDEMGALNIVAKLPRLFQSTHTDLVQVTLKLVFNLSFDGGLRRKMIAAGYLPMLVMFINDEKHHGIAVKILYHMSLDDKVKGMFTQTECVQMATDAIILNLNVKVDLDLIALCINLSLNRRNAQIMVEGQRLHSLMDRAFKYQDALLMKLLRNLSQHES-LQLQFIDYVGDLARILTICD---DEAFIVECLGILGNLALTDLDYSQILQNFQLIPWIRQILLPCARMDDLVLDTIVYLGTCACDELCALLFCRAKVVISLIELLKAKQEDDEIVLQIIFVFQQILRHESTREYMIKETESAAYLIDLMHDKNEEIRKVCDYCLDIIAISDSEWAKRIKLEKFRNHNSQWLCMVES 829          

HSP 2 Score: 208.379 bits (529), Expect = 1.578e-55
Identity = 114/274 (41.61%), Postives = 178/274 (64.96%), Query Frame = 3
Query:  102 KKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI------------------KSKASNKPADPG--------------------AFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR 809
            ++  + G+I+ HPT+KALIVNY+L+AT+FGE  NPM  ++K CQKIIR+++L++ T+   LA+E+V+ C LI  S++ +VE ++ YL +RK+ A ++I                  +S A +  +                       A  +       ++N+ DEY+ELLYE++ ++++ SAMILQ+ARNPDNLEEL  N   + AL+RVLREDW++S DL+TNIIYIFFCFS+++ FH +I+ +KIG+L M++ID+EL++YE   +EL  ++
Sbjct:   11 RRRWKGGSIEPHPTDKALIVNYQLEATVFGEPNNPMIEEKKHCQKIIRLRSLNAKTDPAALAREVVEKCDLIHKSQLNDVEQIIFYLKNRKDNASNDIPDNNTHSRRSAALHTSHTRSSARSHSSVAAMTSSTGSGGGSTGASNGVSNALSAATPTADVSINNIDEYVELLYEELGERIRGSAMILQMARNPDNLEELEKNEACLSALSRVLREDWRKSLDLSTNIIYIFFCFSTYTKFHPLIVQYKIGSLCMDVIDYELRRYETMRNELDVRK 284          
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Match: Kap3 (gene:FBgn0028421 transcript:FBtr0289956)

HSP 1 Score: 385.185 bits (988), Expect = 3.412e-119
Identity = 195/436 (44.72%), Postives = 308/436 (70.64%), Query Frame = 3
Query:  816 QQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG 2123
            +++++D+  K+++   +KQEQL RV++Y+LLN+AE++ LE KMR K++V+ML K L+R+N +LL+L+ +FLKKLSI G+NK ++   N+V +L +   +++ DL+ ++L+L+ N+SFD  LR +++  G LP LV      ++  + + ILY++S +DK K  F +T+C+      I+     KV ++++AL INL  +   A ++ EG+ L  LM RA+K +D ++MK+LRN+SQHE  ++++F+DY+ DLA ++T      D  F+VEC+GIL NL ++DLD+  IL  F L+ W++Q L P    DDL+L+ ++ +GT + D  CA +   A ++ SLI+L+ AKQEDDEIV QI++VF Q++ HESTR+ +I++T++ AY+IDLMHDKN EIRKVCD+ LDII+  D EW+ +I+ EKFR +NSQWL M+E 
Sbjct:  398 KKEEIDRLNKQLKIFAKKQEQLLRVAFYLLLNMAENVKLEEKMRRKHIVKMLVKALDRQNIDLLMLVSTFLKKLSIVGDNKDEMGALNIVAKLPRLFQSTHTDLVQVTLKLVFNLSFDGGLRRKMIAAGYLPMLVMFINDEKHHGIAVKILYHMSLDDKVKGMFTQTECVQMATDAIILNLNVKVDLDLIALCINLSLNRRNAQIMVEGQRLHSLMDRAFKYQDALLMKLLRNLSQHES-LQLQFIDYVGDLARILTICD---DEAFIVECLGILGNLALTDLDYSQILQNFQLIPWIRQILLPCARMDDLVLDTIVYLGTCACDELCALLFCRAKVVISLIELLKAKQEDDEIVLQIIFVFQQILRHESTREYMIKETESAAYLIDLMHDKNEEIRKVCDYCLDIIAISDSEWAKRIKLEKFRNHNSQWLCMVES 829          

HSP 2 Score: 216.468 bits (550), Expect = 2.500e-58
Identity = 118/280 (42.14%), Postives = 183/280 (65.36%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI------------------KSKASNKPADPG--------------------AFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR 809
            DD +++KK  + G+I+ HPT+KALIVNY+L+AT+FGE  NPM  ++K CQKIIR+++L++ T+   LA+E+V+ C LI  S++ +VE ++ YL +RK+ A ++I                  +S A +  +                       A  +       ++N+ DEY+ELLYE++ ++++ SAMILQ+ARNPDNLEEL  N   + AL+RVLREDW++S DL+TNIIYIFFCFS+++ FH +I+ +KIG+L M++ID+EL++YE   +EL  ++
Sbjct:    5 DDAKFIKKRWKGGSIEPHPTDKALIVNYQLEATVFGEPNNPMIEEKKHCQKIIRLRSLNAKTDPAALAREVVEKCDLIHKSQLNDVEQIIFYLKNRKDNASNDIPDNNTHSRRSAALHTSHTRSSARSHSSVAAMTSSTGSGGGSTGASNGVSNALSAATPTADVSINNIDEYVELLYEELGERIRGSAMILQMARNPDNLEELEKNEACLSALSRVLREDWRKSLDLSTNIIYIFFCFSTYTKFHPLIVQYKIGSLCMDVIDYELRRYETMRNELDVRK 284          
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Match: Kap3 (gene:FBgn0028421 transcript:FBtr0343145)

HSP 1 Score: 385.571 bits (989), Expect = 1.794e-118
Identity = 195/436 (44.72%), Postives = 308/436 (70.64%), Query Frame = 3
Query:  816 QQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG 2123
            +++++D+  K+++   +KQEQL RV++Y+LLN+AE++ LE KMR K++V+ML K L+R+N +LL+L+ +FLKKLSI G+NK ++   N+V +L +   +++ DL+ ++L+L+ N+SFD  LR +++  G LP LV      ++  + + ILY++S +DK K  F +T+C+      I+     KV ++++AL INL  +   A ++ EG+ L  LM RA+K +D ++MK+LRN+SQHE  ++++F+DY+ DLA ++T      D  F+VEC+GIL NL ++DLD+  IL  F L+ W++Q L P    DDL+L+ ++ +GT + D  CA +   A ++ SLI+L+ AKQEDDEIV QI++VF Q++ HESTR+ +I++T++ AY+IDLMHDKN EIRKVCD+ LDII+  D EW+ +I+ EKFR +NSQWL M+E 
Sbjct:  490 KKEEIDRLNKQLKIFAKKQEQLLRVAFYLLLNMAENVKLEEKMRRKHIVKMLVKALDRQNIDLLMLVSTFLKKLSIVGDNKDEMGALNIVAKLPRLFQSTHTDLVQVTLKLVFNLSFDGGLRRKMIAAGYLPMLVMFINDEKHHGIAVKILYHMSLDDKVKGMFTQTECVQMATDAIILNLNVKVDLDLIALCINLSLNRRNAQIMVEGQRLHSLMDRAFKYQDALLMKLLRNLSQHES-LQLQFIDYVGDLARILTICD---DEAFIVECLGILGNLALTDLDYSQILQNFQLIPWIRQILLPCARMDDLVLDTIVYLGTCACDELCALLFCRAKVVISLIELLKAKQEDDEIVLQIIFVFQQILRHESTREYMIKETESAAYLIDLMHDKNEEIRKVCDYCLDIIAISDSEWAKRIKLEKFRNHNSQWLCMVES 921          

HSP 2 Score: 214.157 bits (544), Expect = 3.187e-57
Identity = 119/280 (42.50%), Postives = 184/280 (65.71%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI------------------KSKASNKPADPG--------------------AFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR 809
            DD +++KK  + G+I+ HPT+KALIVNY+L+AT+FGE  NPM  ++K CQKIIR+++L++ T+   LA+E+V+ C LI  S++ +VE ++ YL +RK+ A ++I                  +S A +  +                       A  +       ++N+ DEY+ELLYE++ ++++ SAMILQ+ARNPDNLEEL  N+ L  AL+RVLREDW++S DL+TNIIYIFFCFS+++ FH +I+ +KIG+L M++ID+EL++YE   +EL  ++
Sbjct:   98 DDAKFIKKRWKGGSIEPHPTDKALIVNYQLEATVFGEPNNPMIEEKKHCQKIIRLRSLNAKTDPAALAREVVEKCDLIHKSQLNDVEQIIFYLKNRKDNASNDIPDNNTHSRRSAALHTSHTRSSARSHSSVAAMTSSTGSGGGSTGASNGVSNALSAATPTADVSINNIDEYVELLYEELGERIRGSAMILQMARNPDNLEELEKNACL-SALSRVLREDWRKSLDLSTNIIYIFFCFSTYTKFHPLIVQYKIGSLCMDVIDYELRRYETMRNELDVRK 376          
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Match: Kap3 (gene:FBgn0028421 transcript:FBtr0289955)

HSP 1 Score: 385.571 bits (989), Expect = 2.137e-118
Identity = 195/436 (44.72%), Postives = 308/436 (70.64%), Query Frame = 3
Query:  816 QQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG 2123
            +++++D+  K+++   +KQEQL RV++Y+LLN+AE++ LE KMR K++V+ML K L+R+N +LL+L+ +FLKKLSI G+NK ++   N+V +L +   +++ DL+ ++L+L+ N+SFD  LR +++  G LP LV      ++  + + ILY++S +DK K  F +T+C+      I+     KV ++++AL INL  +   A ++ EG+ L  LM RA+K +D ++MK+LRN+SQHE  ++++F+DY+ DLA ++T      D  F+VEC+GIL NL ++DLD+  IL  F L+ W++Q L P    DDL+L+ ++ +GT + D  CA +   A ++ SLI+L+ AKQEDDEIV QI++VF Q++ HESTR+ +I++T++ AY+IDLMHDKN EIRKVCD+ LDII+  D EW+ +I+ EKFR +NSQWL M+E 
Sbjct:  491 KKEEIDRLNKQLKIFAKKQEQLLRVAFYLLLNMAENVKLEEKMRRKHIVKMLVKALDRQNIDLLMLVSTFLKKLSIVGDNKDEMGALNIVAKLPRLFQSTHTDLVQVTLKLVFNLSFDGGLRRKMIAAGYLPMLVMFINDEKHHGIAVKILYHMSLDDKVKGMFTQTECVQMATDAIILNLNVKVDLDLIALCINLSLNRRNAQIMVEGQRLHSLMDRAFKYQDALLMKLLRNLSQHES-LQLQFIDYVGDLARILTICD---DEAFIVECLGILGNLALTDLDYSQILQNFQLIPWIRQILLPCARMDDLVLDTIVYLGTCACDELCALLFCRAKVVISLIELLKAKQEDDEIVLQIIFVFQQILRHESTREYMIKETESAAYLIDLMHDKNEEIRKVCDYCLDIIAISDSEWAKRIKLEKFRNHNSQWLCMVES 922          

HSP 2 Score: 216.853 bits (551), Expect = 3.716e-58
Identity = 118/280 (42.14%), Postives = 183/280 (65.36%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI------------------KSKASNKPADPG--------------------AFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR 809
            DD +++KK  + G+I+ HPT+KALIVNY+L+AT+FGE  NPM  ++K CQKIIR+++L++ T+   LA+E+V+ C LI  S++ +VE ++ YL +RK+ A ++I                  +S A +  +                       A  +       ++N+ DEY+ELLYE++ ++++ SAMILQ+ARNPDNLEEL  N   + AL+RVLREDW++S DL+TNIIYIFFCFS+++ FH +I+ +KIG+L M++ID+EL++YE   +EL  ++
Sbjct:   98 DDAKFIKKRWKGGSIEPHPTDKALIVNYQLEATVFGEPNNPMIEEKKHCQKIIRLRSLNAKTDPAALAREVVEKCDLIHKSQLNDVEQIIFYLKNRKDNASNDIPDNNTHSRRSAALHTSHTRSSARSHSSVAAMTSSTGSGGGSTGASNGVSNALSAATPTADVSINNIDEYVELLYEELGERIRGSAMILQMARNPDNLEELEKNEACLSALSRVLREDWRKSLDLSTNIIYIFFCFSTYTKFHPLIVQYKIGSLCMDVIDYELRRYETMRNELDVRK 377          
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Match: Kap3 (gene:FBgn0028421 transcript:FBtr0343146)

HSP 1 Score: 385.185 bits (988), Expect = 2.453e-118
Identity = 195/436 (44.72%), Postives = 308/436 (70.64%), Query Frame = 3
Query:  816 QQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG 2123
            +++++D+  K+++   +KQEQL RV++Y+LLN+AE++ LE KMR K++V+ML K L+R+N +LL+L+ +FLKKLSI G+NK ++   N+V +L +   +++ DL+ ++L+L+ N+SFD  LR +++  G LP LV      ++  + + ILY++S +DK K  F +T+C+      I+     KV ++++AL INL  +   A ++ EG+ L  LM RA+K +D ++MK+LRN+SQHE  ++++F+DY+ DLA ++T      D  F+VEC+GIL NL ++DLD+  IL  F L+ W++Q L P    DDL+L+ ++ +GT + D  CA +   A ++ SLI+L+ AKQEDDEIV QI++VF Q++ HESTR+ +I++T++ AY+IDLMHDKN EIRKVCD+ LDII+  D EW+ +I+ EKFR +NSQWL M+E 
Sbjct:  491 KKEEIDRLNKQLKIFAKKQEQLLRVAFYLLLNMAENVKLEEKMRRKHIVKMLVKALDRQNIDLLMLVSTFLKKLSIVGDNKDEMGALNIVAKLPRLFQSTHTDLVQVTLKLVFNLSFDGGLRRKMIAAGYLPMLVMFINDEKHHGIAVKILYHMSLDDKVKGMFTQTECVQMATDAIILNLNVKVDLDLIALCINLSLNRRNAQIMVEGQRLHSLMDRAFKYQDALLMKLLRNLSQHES-LQLQFIDYVGDLARILTICD---DEAFIVECLGILGNLALTDLDYSQILQNFQLIPWIRQILLPCARMDDLVLDTIVYLGTCACDELCALLFCRAKVVISLIELLKAKQEDDEIVLQIIFVFQQILRHESTREYMIKETESAAYLIDLMHDKNEEIRKVCDYCLDIIAISDSEWAKRIKLEKFRNHNSQWLCMVES 922          

HSP 2 Score: 216.468 bits (550), Expect = 4.224e-58
Identity = 118/280 (42.14%), Postives = 183/280 (65.36%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI------------------KSKASNKPADPG--------------------AFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR 809
            DD +++KK  + G+I+ HPT+KALIVNY+L+AT+FGE  NPM  ++K CQKIIR+++L++ T+   LA+E+V+ C LI  S++ +VE ++ YL +RK+ A ++I                  +S A +  +                       A  +       ++N+ DEY+ELLYE++ ++++ SAMILQ+ARNPDNLEEL  N   + AL+RVLREDW++S DL+TNIIYIFFCFS+++ FH +I+ +KIG+L M++ID+EL++YE   +EL  ++
Sbjct:   98 DDAKFIKKRWKGGSIEPHPTDKALIVNYQLEATVFGEPNNPMIEEKKHCQKIIRLRSLNAKTDPAALAREVVEKCDLIHKSQLNDVEQIIFYLKNRKDNASNDIPDNNTHSRRSAALHTSHTRSSARSHSSVAAMTSSTGSGGGSTGASNGVSNALSAATPTADVSINNIDEYVELLYEELGERIRGSAMILQMARNPDNLEELEKNEACLSALSRVLREDWRKSLDLSTNIIYIFFCFSTYTKFHPLIVQYKIGSLCMDVIDYELRRYETMRNELDVRK 377          
BLAST of Kinesin-associated protein 3 vs. Ensembl Zebrafish
Match: kifap3a (kinesin-associated protein 3a [Source:ZFIN;Acc:ZDB-GENE-040912-74])

HSP 1 Score: 764.992 bits (1974), Expect = 0.000e+0
Identity = 420/794 (52.90%), Postives = 571/794 (71.91%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQ---------QKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSP--VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
             DD RYLK+  + G++DVHPTEKAL+V YE++ATI GE+G+PM G+RKECQKIIR+K+L+SNT+I  LA+++V+ C+LI PS++PEVE LL YL +RK+ A  + K +   KP D   F+  E++E AN+N+ D+Y+ELLYEDIP+K++ + +IL LARNPDNLEEL  N T +GALARVLREDWK+S DLAT IIYIFFCFSSFS FH +I H+KIG L M+II+HELK+Y++W DELQKK+            +K+ +K LKK   +  KQEQL RV+ Y+LLN++ED   E+KMR KN+V +L KTL+R + ELL+L+VSFLKKLSI+ ENK+D+ + + VE+L+K VP  + DLL+++LRLLLN+SFD++LR ++V+  LLPKL ++        + + ILY+IS +D++K  F  TDCIP +MKM+ +C  E++  E+++  INL  +     ++ EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I  K    + EFV+EC+G LSNLTI DLD+E++L E+NLV ++K +L+P   EDDLILEVV+++GTVS D++CA M+A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+D EW  KIQ EKFRW+NSQWLEM+E      ++D AE P    D G  +  N     DIL R ++  S    +  +P DG++SP    D   G    + G P          +G     RP    GFR D
Sbjct:    3 ADDARYLKRKYKGGSLDVHPTEKALVVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNSNTDIASLARKVVEECRLISPSRLPEVEQLLFYLQNRKKSAEKKEKKQI--KPRDLTPFEGMELEEEANINNIDDYVELLYEDIPEKIRGATLILHLARNPDNLEELLQNETALGALARVLREDWKQSVDLATTIIYIFFCFSSFSQFHGLITHYKIGALCMSIIEHELKRYDLWQDELQKKKKAGDDDPDNQNLKKEYEKALKKYHGLLTKQEQLLRVALYLLLNLSEDTRTELKMRNKNIVHLLVKTLDRDSEELLVLVVSFLKKLSIFLENKNDMAEVDTVEKLAKLVPCEHEDLLNVTLRLLLNLSFDTSLRSKMVQADLLPKLTSMLGDEAQRQIAMRILYHISMDDRFKSMFAYTDCIPQVMKMLFDCGEERIDAELISFCINLAANRRNTQIMCEGNGLKMLMKRALKLKDPLMMKMIRNISQHDGPSKNLFIDYVGDLAAQIGLKE---EEEFVIECLGTLSNLTIPDLDWELVLKEYNLVPYLKDHLKPGSAEDDLILEVVIMIGTVSMDDSCAVMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDVEWGKKIQSEKFRWHNSQWLEMVE----NRQMDEAE-PFMYGDDGEPFLQNG----DILERPDLFYSA---DGIIPADGAISPEFFTDYQNGDLLGQQGFPSSSLTDGYGQTG-VNSARPATAYGFRPD 778          
BLAST of Kinesin-associated protein 3 vs. Ensembl Zebrafish
Match: kifap3b (kinesin-associated protein 3b [Source:ZFIN;Acc:ZDB-GENE-030131-2219])

HSP 1 Score: 759.599 bits (1960), Expect = 0.000e+0
Identity = 396/730 (54.25%), Postives = 541/730 (74.11%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVS-EIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLD---------KTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGA---NTPEL---DPA--EDPAFLYDT-GMNYPYNTVGAD 2216
            D+ +YL++  R G IDVHPTEKAL+V YE++A+I GE GN    ++K+CQKIIR+++L+++T+I  LA+++V+ CKLI PSK+ EVEHLL YL +RK+ +   E K K + KP +   F+  EIDE AN+N  +EY+ELLYE IP+K++ S +IL LARNPDNLEEL  N T +GALARVLREDWK S +LAT IIY+FFCFSSFS FH +I H+KIG L MN+I+HELKKY++W DEL+KK     +D D         KT KK +++  KQEQL RV++Y+LLN++ED   E+KMR KN+V +L K+L+R+N ELL+L+VSFLKKLSI+ ENK+D+ + +VVE+L+  VP  + DL++++LRLL N+SFD+ LR ++V  GLLPKL  L     ++ +V+ ILY+IS +DK K  F  TDCIP LM+M+ E   E++  E+++  INL  +   + ++ EG GL+MLM+RA K +D ++MKM+RNISQH+G +K  F+DY+ DLA  I  K G  D EFV+EC+G ++NLTI DLD+E++L E+NLV ++K +L+P   EDD+ILEVV+++GTVS D++CA M+A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIR+VCD TLDII+E+DEEW  KIQ EKFRW+NSQWLEM+E        P +   DP   E P   Y T GM  P   +  D
Sbjct:    4 DETQYLQRRCRIGDIDVHPTEKALVVYYEVEASILGETGNARHEEKKDCQKIIRLRSLNASTDISALAKKVVEECKLIHPSKLKEVEHLLLYLQNRKKPSTKLEKKEKKAQKPRELLLFEGMEIDEEANINKIEEYIELLYESIPEKIRGSTLILHLARNPDNLEELLHNETALGALARVLREDWKHSVELATTIIYVFFCFSSFSQFHGLITHYKIGALCMNVIEHELKKYDLWQDELEKKSKACDEDPDNHSLRKEHEKTFKKYQSLLVKQEQLLRVAFYLLLNLSEDTRTELKMRNKNIVHLLVKSLDRENEELLVLVVSFLKKLSIFLENKNDMAEMDVVEKLAHLVPCEHEDLMNVTLRLLHNLSFDTGLRTKMVHVGLLPKLTALLGDDTHKLVVMRILYHISVDDKSKSMFAYTDCIPQLMQMLFEHGEERIDTELISFCINLAANKTNSQIMCEGNGLKMLMRRALKLKDPLLMKMIRNISQHDGPLKQLFIDYVGDLAAQI-KKEG--DEEFVIECLGTMANLTIPDLDWELLLKEYNLVPFLKDHLKPGSAEDDMILEVVIMIGTVSMDDSCAVMLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNVEIRQVCDNTLDIIAEYDEEWGKKIQSEKFRWHNSQWLEMVESRQMEDGEPYMYGDDPEALEQPDLFYSTDGMMNPEPGISPD 730          
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Match: kifap3 (kinesin-associated protein 3 [Source:NCBI gene;Acc:100038301])

HSP 1 Score: 773.852 bits (1997), Expect = 0.000e+0
Identity = 440/806 (54.59%), Postives = 584/806 (72.46%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE-VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDK---------TLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRP-VQLANYMRFASG----------DFPYDRPDNRTGFRSDMA 2435
            +DD R+LK+ ++ G IDVHPTEKAL+V+YE++ATI GE G+ M G+RKECQKIIR+K+L++NT+I  LA+++VD CKLI PSK+ EVE LL+YL +R+E +   E K K SNKP DP  F+  EIDE+AN+ND DEY+ELLYE+I DK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++++W +EL KK+     D +          T KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+ + +++E+LSK VP  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + +V+CILY+IS +D++K  F  TDCIP LMKM+ E   E+V +E+++  INL  +   A +I EG GL+MLMK+A K +D ++MKM+RNISQH+G  K  F+DY+ DLA  ITN     + EFV+EC+G L+NLTI+DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD  E+  +LY      P+  +   DIL R ++  S    E  +P DG VSP        F +EF +    L     F +G            P  RP    G+R D A
Sbjct:    3 SDDARFLKRKVKEGNIDVHPTEKALVVHYEVEATILGERGDTMLGERKECQKIIRLKSLNANTDISSLARKVVDECKLIHPSKLAEVEQLLYYLQNRREGLQSKEKKEKKSNKPKDPPPFEGMEIDEVANINDMDEYIELLYENIADKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHDLWQEELSKKKRAVDDDAENQNLKKEYEKTYKKYQGLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFLENKNDMAEMDIIEKLSKLVPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTVLLDNENYKQIVMCILYHISMDDRFKSMFAYTDCIPQLMKMLFESSEERVDLELISFCINLAANKRNAQLICEGNGLKMLMKKALKFKDPLLMKMIRNISQHDGPTKTLFIDYVGDLAAQITNDE---EEEFVIECLGTLANLTITDLDWELVLKEYKLVPYLKDKLKPGSAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLD--ENEQYLYGDEPIEPF--IHERDILERPDLYYSA---EGLIPSDGVVSP-------DFFNEFHQQNGDLMGQHSFNTGMGEDAFGQHTAMP-GRPTTAYGYRPDEA 786          
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Match: kifap3 (kinesin-associated protein 3 [Source:NCBI gene;Acc:100038301])

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 440/801 (54.93%), Postives = 583/801 (72.78%), Query Frame = 3
Query:   87 DVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE-VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQ------QKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRP-VQLANYMRFASG----------DFPYDRPDNRTGFRSDMA 2435
            D RY K  ++ G IDVHPTEKAL+V+YE++ATI GE G+ M G+RKECQKIIR+K+L++NT+I  LA+++VD CKLI PSK+ EVE LL+YL +R+E +   E K K SNKP DP  F+  EIDE+AN+ND DEY+ELLYE+I DK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++++W +EL KK+         +K+ +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+ + +++E+LSK VP  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + +V+CILY+IS +D++K  F  TDCIP LMKM+ E   E+V +E+++  INL  +   A +I EG GL+MLMK+A K +D ++MKM+RNISQH+G  K  F+DY+ DLA  ITN     + EFV+EC+G L+NLTI+DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD  E+  +LY      P+  +   DIL R ++  S    E  +P DG VSP        F +EF +    L     F +G            P  RP    G+R D A
Sbjct:    5 DQRYQK--VKEGNIDVHPTEKALVVHYEVEATILGERGDTMLGERKECQKIIRLKSLNANTDISSLARKVVDECKLIHPSKLAEVEQLLYYLQNRREGLQSKEKKEKKSNKPKDPPPFEGMEIDEVANINDMDEYIELLYENIADKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHDLWQEELSKKKRADILFKNLKKEYEKTYKKYQGLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFLENKNDMAEMDIIEKLSKLVPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTVLLDNENYKQIVMCILYHISMDDRFKSMFAYTDCIPQLMKMLFESSEERVDLELISFCINLAANKRNAQLICEGNGLKMLMKKALKFKDPLLMKMIRNISQHDGPTKTLFIDYVGDLAAQITNDE---EEEFVIECLGTLANLTITDLDWELVLKEYKLVPYLKDKLKPGSAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLD--ENEQYLYGDEPIEPF--IHERDILERPDLYYSA---EGLIPSDGVVSP-------DFFNEFHQQNGDLMGQHSFNTGMGEDAFGQHTAMP-GRPTTAYGYRPDEA 781          
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Match: kifap3 (kinesin-associated protein 3 [Source:NCBI gene;Acc:100038301])

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 435/798 (54.51%), Postives = 577/798 (72.31%), Query Frame = 3
Query:  105 KNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE-VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDK---------TLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRP-VQLANYMRFASG----------DFPYDRPDNRTGFRSDMA 2435
            + ++ G IDVHPTEKAL+V+YE++ATI GE G+ M G+RKECQKIIR+K+L++NT+I  LA+++VD CKLI PSK+ EVE LL+YL +R+E +   E K K SNKP DP  F+  EIDE+AN+ND DEY+ELLYE+I DK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++++W +EL KK+     D +          T KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+ + +++E+LSK VP  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + +V+CILY+IS +D++K  F  TDCIP LMKM+ E   E+V +E+++  INL  +   A +I EG GL+MLMK+A K +D ++MKM+RNISQH+G  K  F+DY+ DLA  ITN     + EFV+EC+G L+NLTI+DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD  E+  +LY      P+  +   DIL R ++  S    E  +P DG VSP        F +EF +    L     F +G            P  RP    G+R D A
Sbjct:   25 RKVKEGNIDVHPTEKALVVHYEVEATILGERGDTMLGERKECQKIIRLKSLNANTDISSLARKVVDECKLIHPSKLAEVEQLLYYLQNRREGLQSKEKKEKKSNKPKDPPPFEGMEIDEVANINDMDEYIELLYENIADKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHDLWQEELSKKKRAVDDDAENQNLKKEYEKTYKKYQGLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFLENKNDMAEMDIIEKLSKLVPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTVLLDNENYKQIVMCILYHISMDDRFKSMFAYTDCIPQLMKMLFESSEERVDLELISFCINLAANKRNAQLICEGNGLKMLMKKALKFKDPLLMKMIRNISQHDGPTKTLFIDYVGDLAAQITNDE---EEEFVIECLGTLANLTITDLDWELVLKEYKLVPYLKDKLKPGSAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLD--ENEQYLYGDEPIEPF--IHERDILERPDLYYSA---EGLIPSDGVVSP-------DFFNEFHQQNGDLMGQHSFNTGMGEDAFGQHTAMP-GRPTTAYGYRPDEA 800          
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Match: kifap3 (kinesin-associated protein 3 [Source:NCBI gene;Acc:100038301])

HSP 1 Score: 757.288 bits (1954), Expect = 0.000e+0
Identity = 436/791 (55.12%), Postives = 577/791 (72.95%), Query Frame = 3
Query:  117 TGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE-VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQ------QKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRP-VQLANYMRFASG----------DFPYDRPDNRTGFRSDMA 2435
             G IDVHPTEKAL+V+YE++ATI GE G+ M G+RKECQKIIR+K+L++NT+I  LA+++VD CKLI PSK+ EVE LL+YL +R+E +   E K K SNKP DP  F+  EIDE+AN+ND DEY+ELLYE+I DK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++++W +EL KK+         +K+ +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+ + +++E+LSK VP  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + +V+CILY+IS +D++K  F  TDCIP LMKM+ E   E+V +E+++  INL  +   A +I EG GL+MLMK+A K +D ++MKM+RNISQH+G  K  F+DY+ DLA  ITN     + EFV+EC+G L+NLTI+DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD  E+  +LY      P+  +   DIL R ++  S    E  +P DG VSP        F +EF +    L     F +G            P  RP    G+R D A
Sbjct:    6 AGNIDVHPTEKALVVHYEVEATILGERGDTMLGERKECQKIIRLKSLNANTDISSLARKVVDECKLIHPSKLAEVEQLLYYLQNRREGLQSKEKKEKKSNKPKDPPPFEGMEIDEVANINDMDEYIELLYENIADKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHDLWQEELSKKKRADILFKNLKKEYEKTYKKYQGLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFLENKNDMAEMDIIEKLSKLVPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTVLLDNENYKQIVMCILYHISMDDRFKSMFAYTDCIPQLMKMLFESSEERVDLELISFCINLAANKRNAQLICEGNGLKMLMKKALKFKDPLLMKMIRNISQHDGPTKTLFIDYVGDLAAQITNDE---EEEFVIECLGTLANLTITDLDWELVLKEYKLVPYLKDKLKPGSAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLD--ENEQYLYGDEPIEPF--IHERDILERPDLYYSA---EGLIPSDGVVSP-------DFFNEFHQQNGDLMGQHSFNTGMGEDAFGQHTAMP-GRPTTAYGYRPDEA 774          
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Match: kifap3 (kinesin-associated protein 3 [Source:NCBI gene;Acc:100038301])

HSP 1 Score: 752.666 bits (1942), Expect = 0.000e+0
Identity = 434/787 (55.15%), Postives = 575/787 (73.06%), Query Frame = 3
Query:  129 DVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE-VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQ------QKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRP-VQLANYMRFASG----------DFPYDRPDNRTGFRSDMA 2435
            DVHPTEKAL+V+YE++ATI GE G+ M G+RKECQKIIR+K+L++NT+I  LA+++VD CKLI PSK+ EVE LL+YL +R+E +   E K K SNKP DP  F+  EIDE+AN+ND DEY+ELLYE+I DK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++++W +EL KK+         +K+ +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+ + +++E+LSK VP  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + +V+CILY+IS +D++K  F  TDCIP LMKM+ E   E+V +E+++  INL  +   A +I EG GL+MLMK+A K +D ++MKM+RNISQH+G  K  F+DY+ DLA  ITN     + EFV+EC+G L+NLTI+DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD  E+  +LY      P+  +   DIL R ++  S    E  +P DG VSP        F +EF +    L     F +G            P  RP    G+R D A
Sbjct:    7 DVHPTEKALVVHYEVEATILGERGDTMLGERKECQKIIRLKSLNANTDISSLARKVVDECKLIHPSKLAEVEQLLYYLQNRREGLQSKEKKEKKSNKPKDPPPFEGMEIDEVANINDMDEYIELLYENIADKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHDLWQEELSKKKRADILFKNLKKEYEKTYKKYQGLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFLENKNDMAEMDIIEKLSKLVPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTVLLDNENYKQIVMCILYHISMDDRFKSMFAYTDCIPQLMKMLFESSEERVDLELISFCINLAANKRNAQLICEGNGLKMLMKKALKFKDPLLMKMIRNISQHDGPTKTLFIDYVGDLAAQITNDE---EEEFVIECLGTLANLTITDLDWELVLKEYKLVPYLKDKLKPGSAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLD--ENEQYLYGDEPIEPF--IHERDILERPDLYYSA---EGLIPSDGVVSP-------DFFNEFHQQNGDLMGQHSFNTGMGEDAFGQHTAMP-GRPTTAYGYRPDEA 771          
BLAST of Kinesin-associated protein 3 vs. Ensembl Mouse
Match: Kifap3 (kinesin-associated protein 3 [Source:MGI Symbol;Acc:MGI:107566])

HSP 1 Score: 790.415 bits (2040), Expect = 0.000e+0
Identity = 439/809 (54.26%), Postives = 588/809 (72.68%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            +D RYLK+ ++ G IDVHP+EKALIV YE++ATI GE+G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD DKT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I++     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+P+ +              RP    GFR D
Sbjct:    4 EDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISSDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLG--MDGFGQPLGILG------------RPATAYGFRPD 783          
BLAST of Kinesin-associated protein 3 vs. Ensembl Mouse
Match: Kifap3 (kinesin-associated protein 3 [Source:MGI Symbol;Acc:MGI:107566])

HSP 1 Score: 786.563 bits (2030), Expect = 0.000e+0
Identity = 426/748 (56.95%), Postives = 572/748 (76.47%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP 2297
            +D RYLK+ ++ G IDVHP+EKALIV YE++ATI GE+G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD DKT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I++     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP
Sbjct:    4 EDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISSDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLITSEGAISP 736          
BLAST of Kinesin-associated protein 3 vs. UniProt/SwissProt
Match: sp|Q26626|KIFA3_STRPU (Kinesin-associated protein 3 OS=Strongylocentrotus purpuratus OX=7668 GN=KAP115 PE=1 SV=1)

HSP 1 Score: 840.106 bits (2169), Expect = 0.000e+0
Identity = 443/828 (53.50%), Postives = 602/828 (72.71%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVA--VSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDE--FGRPVQLANYMRFASGDFPYDRPDNRTGFRSDMATIEHSMAVDKFGRPLIMDVNDEALQDYVGQYAR 2534
             +D RYLK+ ++ G+IDVHPTEKAL+VNYEL+ATI GE+G+PM G+RKECQKIIR+++L+S T+I  LA+E+V+ CKLI PSK+PEVE LL+YL +RKE        K + + K  DP  ++ TE++E+AN+ND ++Y+ELLYED P+K++ +A+ILQLARNPDNLEEL  N T++GALARVLRE+WK S +LATNI+YIFFCFSSFS FH I+ HFKIG L M +++HELKK+  W +EL KK+         ++D +K+ KK   + +KQEQL RVS+Y+LLN++ED  +E+KM+ KN++ +L KTLER N ELLIL+VSFLKKL IY ENK+++ +  ++ERL+K VP  + DLL+I+LRLLLN+SFD+ LR +++K G+LPK V L  +  +  +VLC+LY++S +DK K  F  TDCIP +MKM+LE   E++ IE+VAL INL C+   A +I EG GLR+LM+RA K RD +++KM+RNISQH+G  K +F++YISD+A +I   +   D E VVE +GI++NLTI DLD+EMI+ EF LV W+K+ L+P   EDDL+LEVVMLVGTV+ D++CA M+A +GIIQSLI+L+ AKQEDDEIVCQIVYVFYQ++FHE+TR++II+ TQAPAY+IDLMHDKN+EIRKVCD TLDIISEFDEEW+ KI+ EKFRW+NSQWLEM+E    + ++D  E    +Y      PY  +   DIL R ++       E+G   DG V+P      G+  +    GRP       R+ S ++   RP +R  + +     E    +D++G P+      E   + V QY R
Sbjct:    3 AEDARYLKRKVKGGSIDVHPTEKALVVNYELEATILGEMGDPMLGERKECQKIIRLRSLNSTTDIAALAREVVEKCKLIHPSKLPEVEQLLYYLQNRKETTQKAGAKKGELTGKLKDPPPYEGTELNEVANINDIEDYIELLYEDNPEKVRGTALILQLARNPDNLEELQSNETVLGALARVLREEWKHSVELATNIVYIFFCFSSFSQFHSIVAHFKIGALVMGVVEHELKKHLSWNEELLKKKKTAEENPASKRDFEKSTKKYHGLLKKQEQLLRVSFYLLLNLSEDPKVELKMKNKNIIRLLIKTLERDNAELLILVVSFLKKLGIYVENKNEMAEQQIIERLAKLVPCDHEDLLNITLRLLLNLSFDTDLRGKMIKLGMLPKFVELLANDNHRLVVLCVLYHVSQDDKAKSMFTYTDCIPMIMKMMLESPTERLEIELVALGINLACNKHNAQLICEGNGLRLLMRRALKFRDPLLLKMIRNISQHDGPSKAQFIEYISDVARIIKESN---DEELVVEALGIMANLTIPDLDYEMIINEFGLVPWIKEKLEPGAAEDDLVLEVVMLVGTVATDDSCAAMLAKSGIIQSLIELLNAKQEDDEIVCQIVYVFYQMVFHEATREVIIKLTQAPAYLIDLMHDKNSEIRKVCDNTLDIISEFDEEWARKIKLEKFRWHNSQWLEMVE----SQQIDDGEG-GLMYGDESYSPY--IQESDILDRPDLFYGQTGYEDGEMYDGQVTPEYVDEYGNVQNGEYLGRPASAMYGNRYQS-EYESLRPGSRVAYLT-----EDGQPLDEYGNPI------EVYAESVDQYGR 808          
BLAST of Kinesin-associated protein 3 vs. UniProt/SwissProt
Match: sp|Q92845|KIFA3_HUMAN (Kinesin-associated protein 3 OS=Homo sapiens OX=9606 GN=KIFAP3 PE=1 SV=2)

HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0
Identity = 439/809 (54.26%), Postives = 589/809 (72.81%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            +D RYLK+ ++ G IDVHP+EKALIV+YE++ATI GE+G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD +KT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I+N     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + ++D +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+PV +              RP    GFR D
Sbjct:    4 EDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQMDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLG--MDGFGQPVGILG------------RPATAYGFRPD 783          
BLAST of Kinesin-associated protein 3 vs. UniProt/SwissProt
Match: sp|P70188|KIFA3_MOUSE (Kinesin-associated protein 3 OS=Mus musculus OX=10090 GN=Kifap3 PE=1 SV=1)

HSP 1 Score: 790.415 bits (2040), Expect = 0.000e+0
Identity = 439/809 (54.26%), Postives = 588/809 (72.68%), Query Frame = 3
Query:   84 DDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS------EQQ---KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP-----------------VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
            +D RYLK+ ++ G IDVHP+EKALIV YE++ATI GE+G+PM G+RKECQKIIR+K+L++NT+I  LA+++V+ CKLI PSK+ EVE LL+YL +R++    + K + S+KP DP  F+  EIDE+AN+ND DEY+ELLYEDIPDK++ SA+ILQLARNPDNLEEL  N T +GALARVLREDWK+S +LATNIIYIFFCFSSFS FH +I H+KIG L MNIIDHELK++E+W +EL KK+       E Q   KD DKT KK + +  KQEQL RV+ Y+LLN+AED   E+KMR KN+V ML K L+R N ELLIL+VSFLKKLSI+ ENK+D+V+ ++VE+L K +P  + DLL+I+LRLLLN+SFD+ LR ++V+ GLLPKL  L  +   + + +C+LY+IS +D++K  F  TDCIP LMKM+ EC  E++ +E+++  INL  +     +I EG GL+MLMKRA K +D ++MKM+RNISQH+G  K  F+DY+ DLA  I++     + EFV+EC+G L+NLTI DLD+E++L E+ LV ++K  L+P   EDDL+LEVV+++GTVS D++CA ++A +GII +LI+L+ A+QEDDE VCQI+YVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW+ KIQ EKFRW+NSQWLEM+E    + +LD +E   +LY      PY   G  DIL R ++     Y+ +GL   +G++SP                     SLG   D FG+P+ +              RP    GFR D
Sbjct:    4 EDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISSDE---EEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVE----SRQLDESE--QYLYGDDRIEPYIHEG--DILERPDLF----YNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLG--MDGFGQPLGILG------------RPATAYGFRPD 783          
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Match: A0A210R5E3 (Kinesin-associated protein 3 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT03659 PE=4 SV=1)

HSP 1 Score: 860.522 bits (2222), Expect = 0.000e+0
Identity = 457/830 (55.06%), Postives = 615/830 (74.10%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEI--KSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS--------EQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGF--RSDMATIEHSMAVDKFGRPLIMDVNDEALQDYVGQYAR 2534
             +D RYLK+ ++ GTID+HPTEKAL+VNYEL+ATI GELG+PM G+RKECQKIIRVK+L+ N+++  LA+EI+D CKLI PSK+PEVE LL+YL +RKE   ++   K++   KP  P  +D+TEIDE+AN+ND D+Y+ELLYED+P+K++ SA+ILQLARNPDNLEEL+ N T++GALARVLREDWK++T+LATNI+Y FFCFSSF+ FH +I+HFKIG L MN+++HELKKY+MW +ELQ+KR         + ++DL+K+L+K + + +KQEQL RV++Y+LLN++ED+ +E+KMR KN+V+ML +++ER N ELLIL+VSFLKKLSI+ ENK ++   N++ER  K VP  + DLL+I+LRLLLN+SFD+ LR +++K GLLPKLV L  +  +  +VLCILY++S +DK K  F  TDCIP +MKM LE   E   +E++AL INL  +   A +I EG GL+MLMKRA+K +D ++MKM+RNISQH+G  K  F+DYISDLAE   N     D EF +E +GIL NLTI DLD+E+IL E+NLV W+K+ L P   EDD++L+V++LVGTV ND+ CAKM+A++ IIQ LI+L+ AKQEDDE+VCQIVYVFYQ+IFHESTR++II+ TQAPAY+IDLMHDKN EIRKVCD TLDII+EFDEEW+ KIQ EKFRW+NSQWLEM+E    T +++   DP ++Y      PY  +   DIL R ++     YD NG+ +DG ++P      G +    G+P           G  PY RP++  GF      AT ++   +D + +P++    D   Q  V +Y R
Sbjct:    3 AEDARYLKRKVKGGTIDIHPTEKALVVNYELEATILGELGDPMLGERKECQKIIRVKSLNPNSDLSSLAKEIIDKCKLIHPSKLPEVEQLLYYLQNRKESEPTKASGKTQLQEKPEVP-TYDATEIDEVANINDVDDYMELLYEDVPEKIRGSALILQLARNPDNLEELFQNETVVGALARVLREDWKKNTELATNIVYTFFCFSSFTQFHGVILHFKIGALCMNVVEHELKKYDMWNEELQRKRKTAEGEKDKKTKEDLEKSLQKYDGLVKKQEQLLRVAFYLLLNLSEDLKVEMKMRNKNVVKMLVRSMERDNFELLILVVSFLKKLSIFIENKQEMATMNIMERFVKIVPCEHEDLLNITLRLLLNLSFDADLRSKMIKFGLLPKLVGLLNNENHRLIVLCILYHVSMDDKSKSMFTYTDCIPMVMKMFLESP-ENPDLELLALCINLAANKRTAQIICEGSGLKMLMKRAFKFKDPLLMKMIRNISQHDGPTKNLFIDYISDLAEATLNCD---DEEFALEALGILGNLTIPDLDYELILREYNLVPWIKERLMPGSAEDDIVLQVIILVGTVCNDDACAKMLAESNIIQCLIELLNAKQEDDEMVCQIVYVFYQMIFHESTREVIIKDTQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEFDEEWAKKIQLEKFRWHNSQWLEMVE----TRQMEDMADP-YMYPEDEYDPY--LQDADILDRPDLFYGDAYD-NGI-MDGHLTPEYIDENGVYD---GKPYGYGYGADADDGYEPYARPESHVGFVMEGSQATDDYGNLLDPYSQPMV----DPYSQPVVDEYGR 811          
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Match: A0A2R2MLN0 (kinesin-associated protein 3-like OS=Lingula unguis OX=7574 GN=LOC112041747 PE=4 SV=1)

HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0
Identity = 468/848 (55.19%), Postives = 606/848 (71.46%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEV---AVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR--------SEQQK-DLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEML---------MSGMYDE--NGL--PVDGSVSPVLDPSLGSFTDEFGRPVQLANY-MRFASGDFPYDRPDNRTGFRSDMATIEHSMA---------VDKFGRPLIMDVNDEALQDYV 2519
             +D R+LK+ ++ G IDVHPTEKAL+V+YEL+ATI GELG+PM G+RKECQKIIRVK L+ NTNI  LA+EI+D CKLI PSK+PEVE LL+YL  RK+         K   S KP D   F+ TEIDE ANLND DEYLELLYED+P+KL+ SA+ LQLARNPDNLEE++ N T++GALARVLREDW++S++LATNIIYIFFCFSSFS FH +I HFKIG  TM II+HELKKYE+W  EL KKR        SE+ K D +K+ KK + +  KQEQL RVS+Y+LLN++ED S+E KMR K +V ML K LER N ELLIL+VSFLKKLSI+ ENK+++ +  +VERLS+ VP  + DLL+I+LRLLLN+SFD   R ++VK GLLPK V L  +  ++ +VLCILY++S +DK K  F  TDCIP +MKMILE   ++V +E++AL INL  +   A +I EG+GL++L+KRAYK +D ++MKM+RNISQH+G+ K  F+DY+SDLA  I       D EFV+ECVGIL N+T  DLD+E++L E+ LV W++  +QP   EDD++LE+V+L+GTV  D+ CAKM+AD GIIQ+LI+L+ AKQEDDE+VCQI+YVFYQ+IFHE+TR+III++TQAPAY+IDLMHDKN EIRKVCD TLDII EF+E+W  KIQ EKFRW+NSQWLEM+E    + +++  E  AFLY  G +   + V   DIL R ++          M G Y +  +G    +DG+V+P        + DE G  +    Y MR +     Y RPD   GF  D  T++   A         VD++GRPL+ D       +Y+
Sbjct:    3 AEDARFLKRKVKGGNIDVHPTEKALVVHYELEATILGELGDPMLGERKECQKIIRVKTLNQNTNIAALAREIIDKCKLIHPSKLPEVEQLLYYLQKRKDSPPPGKGNEKKTLSEKPLDQQTFEGTEIDETANLNDVDEYLELLYEDLPEKLRGSALFLQLARNPDNLEEIFQNETVVGALARVLREDWQKSSELATNIIYIFFCFSSFSQFHGVISHFKIGARTMGIIEHELKKYELWKQELDKKRKAAEADKNSEKAKADYEKSEKKFKGLLAKQEQLLRVSFYLLLNLSEDTSVEKKMRNKGIVIMLVKCLERDNFELLILVVSFLKKLSIFVENKNEMAEAGIVERLSRLVPCEHEDLLNITLRLLLNLSFDQEQRGKMVKVGLLPKFVGLLGNENHKLIVLCILYHLSLDDKCKSLFTYTDCIPIVMKMILESPGDRVDLELIALGINLAANKRNAQMICEGQGLKLLLKRAYKFKDPLLMKMIRNISQHDGQTKELFIDYVSDLANAIQKIE---DEEFVIECVGILGNVTFPDLDYELLLKEYQLVPWIQTKMQPGAAEDDMVLEIVILLGTVCCDDNCAKMLADNGIIQNLIELLNAKQEDDEVVCQIIYVFYQMIFHEATREIIIKQTQAPAYLIDLMHDKNPEIRKVCDQTLDIIGEFEEDWRKKIQLEKFRWHNSQWLEMVE----SRQIEDNE--AFLY--GDDSFGSYVQDSDILDRPDLFYGSPGDVLEMGGYYGDYLHGFDPTLDGNVTP-------EYMDEQGYMMGGVPYGMRVSDEYESYARPDAHPGFFMDGQTVDEYGAPIDDYGTVMVDQYGRPLVDDYGRPIPPEYI 832          
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Match: A0A267DQM3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016361g2 PE=4 SV=1)

HSP 1 Score: 836.639 bits (2160), Expect = 0.000e+0
Identity = 441/833 (52.94%), Postives = 604/833 (72.51%), Query Frame = 3
Query:   75 NMTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS-------EQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNA--DXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVIS--EGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGE-IKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQP-TKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGD----FPYDRPDNRTGFR-------SDMATIEHSMAVDKFGRPLIMDVNDE 2501
            N   D RYLK+ +R G IDVHPTEKAL+VNYEL+ATI  E GNPM  D+KECQK+IRVKNLD NT+IPKLA+EIV+ C+LI PSK+PEVEHLLHYL +RK   V++ K+K S KP+DPGAF+STEIDE AN+ D ++Y+ELLYEDIP+K+ ASA+ILQLARNPDNLEEL++N TLIGALARVL+EDWK S DL+ NIIY+FFCFSSFS+FH +I HF+IG   M +++HEL+KYEMW +EL +KR        + ++DL+K  +K +T+ +KQEQLFRVS+YMLLN++E++S+E+KM+ K +V ML +TL+R N EL ILIVSF+KKLSI+ EN  +L+K NV+ERL   +       DLL+I+LRL+LN++FD  +R ++VK G+L  L+       ++ +VLCILY++S +D++K  F  TDC+  L+K+I E + E + IE+++LA+NL CD  +A V++  +GKG+R+ MKRA++ +D ++MKMLRNIS+HE E +KM F++YISDL  VI +  G +  +F +EC+GIL+NL I DLDF +I+ E++LV+W+++ LQP  +  DD++L+V++LVGT+ ND++CA+++A+A ++Q+LI+L+ AKQEDDE+VCQIV+VFYQLIFHE+TR I+I++TQAPAY+IDLMHDKN  IR+VCD TLDIISEFD +W  +IQ EKF+W+NSQWL+M+EGG       PA  P               G DD            +D  G   D +++    P L  + D+  R    A       GD     PYDRP N  GF        +D A +   MA D+F RP+  D+ D+
Sbjct:    2 NPDSDARYLKRKVRGGNIDVHPTEKALVVNYELEATILSEEGNPMLEDKKECQKLIRVKNLDENTDIPKLAKEIVERCRLIHPSKLPEVEHLLHYLANRKTEYVAQAKTKLSEKPSDPGAFESTEIDEQANIVDVEDYMELLYEDIPEKVHASALILQLARNPDNLEELFNNETLIGALARVLKEDWKSSYDLSINIIYVFFCFSSFSNFHPVIAHFRIGAQVMTVLEHELQKYEMWKEELARKRQAAAGSGKDGKRDLEKATRKFKTLVKKQEQLFRVSFYMLLNISEELSVEVKMKAKGIVPMLCRTLDRDNYELQILIVSFIKKLSIFKENIDELLKVNVMERLHHVLKQFKTCDDLLNITLRLILNLTFDRRMRAQLVKIGMLQTLMQYMMHENHKNIVLCILYHVSHDDEHKAKFFHTDCVSLLVKLITESREELIDIEVMSLAVNLACDPTLAEVLAGKDGKGIRIFMKRAFRFKDSVLMKMLRNISEHESEKLKMLFVNYISDLGRVIVD-PGDYGDDFALECLGILANLNIPDLDFRLIIEEYDLVEWIRKQLQPGAQTPDDVVLQVIILVGTICNDDSCARLLAEAQVVQTLIELLNAKQEDDEVVCQIVFVFYQLIFHETTRNILIKETQAPAYLIDLMHDKNVAIRQVCDLTLDIISEFDPDWGRRIQLEKFKWHNSQWLDMMEGGQ------PAAGPGL------------GGVDD------------FDPRGYDGDEALN---HPEL-FYGDDLARVTGAATPDMIYPGDEGPPRPYDRPPNGMGFSPMDATSLADQAMMGGLMAYDEFDRPISADLLDQ 799          
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Match: A0A267GUR2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016361g1 PE=4 SV=1)

HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0
Identity = 440/833 (52.82%), Postives = 604/833 (72.51%), Query Frame = 3
Query:   75 NMTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS-------EQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNA--DXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVIS--EGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGE-IKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQP-TKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGD----FPYDRPDNRTGFR-------SDMATIEHSMAVDKFGRPLIMDVNDE 2501
            N   D RYLK+ +R G IDVHPTEKAL+VNYEL+ATI  E GNPM  D+KECQK+IRVKNLD NT+IPKLA+EIV+ C+LI PSK+PEVEHLLHYL +RK   V++ K+K S KP+DPGAF+STEIDE AN+ D ++Y+ELLYEDIP+K+ ASA+ILQLARNPDNLEEL++N TLIGALARVL+EDWK S DL+ NIIY+FFCFSSFS+FH +I HF+IG   M +++HEL+KY+MW +EL +KR        + ++DL+K  +K +T+ +KQEQLFRVS+YMLLN++E++S+E+KM+ K +V ML +TL+R N EL ILIVSF+KKLSI+ EN  +L+K NV+ERL   +       DLL+I+LRL+LN++FD  +R ++VK G+L  L+       ++ +VLCILY++S +D++K  F  TDC+  L+K+I E + E + IE+++LA+NL CD  +A V++  +GKG+R+ MKRA++ +D ++MKMLRNIS+HE E +KM F++YISDL  VI +  G +  +F +EC+GIL+NL I DLDF +I+ E++LV+W+++ LQP  +  DD++L+V++LVGT+ ND++CA+++A+A ++Q+LI+L+ AKQEDDE+VCQIV+VFYQLIFHE+TR I+I++TQAPAY+IDLMHDKN  IR+VCD TLDIISEFD +W  +IQ EKF+W+NSQWL+M+EGG       PA  P               G DD            +D  G   D +++    P L  + D+  R    A       GD     PYDRP N  GF        +D A +   MA D+F RP+  D+ D+
Sbjct:    2 NPDSDARYLKRKVRGGNIDVHPTEKALVVNYELEATILSEEGNPMLEDKKECQKLIRVKNLDENTDIPKLAKEIVERCRLIHPSKLPEVEHLLHYLANRKTEYVAQAKTKLSEKPSDPGAFESTEIDEQANIVDVEDYMELLYEDIPEKVHASALILQLARNPDNLEELFNNETLIGALARVLKEDWKSSYDLSINIIYVFFCFSSFSNFHPVIAHFRIGAQVMTVLEHELQKYDMWKEELARKRQAAAGSGKDGKRDLEKATRKFKTLVKKQEQLFRVSFYMLLNISEELSVEVKMKAKGIVPMLCRTLDRDNYELQILIVSFIKKLSIFKENIDELLKVNVMERLHHVLKQFKTCDDLLNITLRLILNLTFDRRMRAQLVKIGMLQTLMQYMMHENHKNIVLCILYHVSHDDEHKAKFFHTDCVSLLVKLITESREELIDIEVMSLAVNLACDPTLAEVLAGKDGKGIRIFMKRAFRFKDSVLMKMLRNISEHESEKLKMLFVNYISDLGRVIVD-PGDYGDDFALECLGILANLNIPDLDFRLIIEEYDLVEWIRKQLQPGAQTPDDVVLQVIILVGTICNDDSCARLLAEAQVVQTLIELLNAKQEDDEVVCQIVFVFYQLIFHETTRNILIKETQAPAYLIDLMHDKNVAIRQVCDLTLDIISEFDPDWGRRIQLEKFKWHNSQWLDMMEGGQ------PAAGPGL------------GGVDD------------FDPRGYDGDEALN---HPEL-FYGDDLARVTGAATPDMIYPGDEGPPRPYDRPPNGMGFSPMDATSLADQAMMGGLMAYDEFDRPISADLLDQ 799          
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Match: A0A1I8I5H2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0
Identity = 440/833 (52.82%), Postives = 604/833 (72.51%), Query Frame = 3
Query:   75 NMTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRS-------EQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNA--DXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVIS--EGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGE-IKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQP-TKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGD----FPYDRPDNRTGFR-------SDMATIEHSMAVDKFGRPLIMDVNDE 2501
            N   D RYLK+ +R G IDVHPTEKAL+VNYEL+ATI  E GNPM  D+KECQK+IRVKNLD NT+IPKLA+EIV+ C+LI PSK+PEVEHLLHYL +RK   V++ K+K S KP+DPGAF+STEIDE AN+ D ++Y+ELLYEDIP+K+ ASA+ILQLARNPDNLEEL++N TLIGALARVL+EDWK S DL+ NIIY+FFCFSSFS+FH +I HF+IG   M +++HEL+KY+MW +EL +KR        + ++DL+K  +K +T+ +KQEQLFRVS+YMLLN++E++S+E+KM+ K +V ML +TL+R N EL ILIVSF+KKLSI+ EN  +L+K NV+ERL   +       DLL+I+LRL+LN++FD  +R ++VK G+L  L+       ++ +VLCILY++S +D++K  F  TDC+  L+K+I E + E + IE+++LA+NL CD  +A V++  +GKG+R+ MKRA++ +D ++MKMLRNIS+HE E +KM F++YISDL  VI +  G +  +F +EC+GIL+NL I DLDF +I+ E++LV+W+++ LQP  +  DD++L+V++LVGT+ ND++CA+++A+A ++Q+LI+L+ AKQEDDE+VCQIV+VFYQLIFHE+TR I+I++TQAPAY+IDLMHDKN  IR+VCD TLDIISEFD +W  +IQ EKF+W+NSQWL+M+EGG       PA  P               G DD            +D  G   D +++    P L  + D+  R    A       GD     PYDRP N  GF        +D A +   MA D+F RP+  D+ D+
Sbjct:    2 NPDSDARYLKRKVRGGNIDVHPTEKALVVNYELEATILSEEGNPMLEDKKECQKLIRVKNLDENTDIPKLAKEIVERCRLIHPSKLPEVEHLLHYLANRKTEYVAQAKTKLSEKPSDPGAFESTEIDEQANIVDVEDYMELLYEDIPEKVHASALILQLARNPDNLEELFNNETLIGALARVLKEDWKSSYDLSINIIYVFFCFSSFSNFHPVIAHFRIGAQVMTVLEHELQKYDMWKEELARKRQAAAGSGKDGKRDLEKATRKFKTLVKKQEQLFRVSFYMLLNISEELSVEVKMKAKGIVPMLCRTLDRDNYELQILIVSFIKKLSIFKENIDELLKVNVMERLHHVLKQFKTCDDLLNITLRLILNLTFDRRMRAQLVKIGMLQTLMQYMMHENHKNIVLCILYHVSHDDEHKAKFFHTDCVSLLVKLITESREELIDIEVMSLAVNLACDPTLAEVLAGKDGKGIRIFMKRAFRFKDSVLMKMLRNISEHESEKLKMLFVNYISDLGRVIVD-PGDYGDDFALECLGILANLNIPDLDFRLIIEEYDLVEWIRKQLQPGAQTPDDVVLQVIILVGTICNDDSCARLLAEAQVVQTLIELLNAKQEDDEVVCQIVFVFYQLIFHETTRNILIKETQAPAYLIDLMHDKNVAIRQVCDLTLDIISEFDPDWGRRIQLEKFKWHNSQWLDMMEGGQ------PAAGPGL------------GGVDD------------FDPRGYDGDEALN---HPEL-FYGDDLARVTGAATPDMIYPGDEGPPRPYDRPPNGMGFSPMDATSLADQAMMGGLMAYDEFDRPISADLLDQ 799          
BLAST of Kinesin-associated protein 3 vs. Ensembl Cavefish
Match: kifap3a (kinesin associated protein 3 [Source:NCBI gene;Acc:103028328])

HSP 1 Score: 770 bits (1987), Expect = 0.000e+0
Identity = 416/796 (52.26%), Postives = 568/796 (71.36%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQ---------KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLY-DTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP--VLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSD 2429
             DDVRYL + ++ G++DVHPTE+AL+VNYE++A I G++G+ M G+RK CQKIIR+K+L+ NT+I  LA+++V+ C+LI P+K+PEVE LL YL +RK  + +E K K   KP DP  F+  E+DE AN+N  D+Y+ELLYED+P+K++ + +IL LARNPDNLEEL  N T +GALARVLREDWK+S DLAT IIY+FFCFSSF  FH +I H+KIG L MNII+HELK+Y++W +ELQKK+   +         K+ +K LKK + +  KQEQL RV+ Y+LLN++ED   E+KMR KN+V +L KTL+R + ELL+L+VSFLKKLSI+ ENK+D+ + + VE+L+K VP  + DLL+++LRLL N+SFD+ LR ++V+ GLLPKL  L        L + ILY+IS +D++K  F  TDCIP +MKM+ +C  E++  E+++  INL  +   A +I EG GL+MLMKRA K +D ++MKM+RNISQH+G     F+DY+ DLA  I       + EF++EC+G L+NLTI DLD+E++L E+NLV ++K +L+P   EDDL+LEVV+++GTVS D++CA M+A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW  KIQ EKFRW+NSQWLEM+E      ++D AE   F+Y D G  +    +   DIL R ++     Y  +GL   D ++SP    D   G F    G P    +         P  RP    GFR D
Sbjct:    3 ADDVRYLTRKVKGGSLDVHPTERALVVNYEVEARILGQMGDAMLGERKACQKIIRLKSLNPNTDIGSLARKVVEECRLISPTKLPEVEQLLFYLQNRK--SPTEKKEKKQIKPRDPTPFEGMELDEEANINSIDDYVELLYEDVPEKIRGATLILHLARNPDNLEELLQNETALGALARVLREDWKQSVDLATTIIYVFFCFSSFIQFHGLITHYKIGALCMNIIEHELKRYDLWQEELQKKKKAGEDDPENQNLKKEYEKALKKYQGLLTKQEQLLRVALYLLLNLSEDTRTELKMRNKNIVHLLVKTLDRDSEELLVLVVSFLKKLSIFLENKNDMAETDTVEKLAKLVPCDHEDLLNVTLRLLHNLSFDTGLRSKMVQVGLLPKLTALLGDEAQRHLSMRILYHISMDDRFKSMFAYTDCIPQVMKMLFDCGEERMDPELISFCINLAANKRNAQLICEGNGLKMLMKRALKLKDPLMMKMIRNISQHDGPSMNLFIDYVGDLAAQIGQNE---EEEFMIECLGTLANLTIPDLDWELVLKEYNLVPFLKDHLKPGCAEDDLVLEVVIMIGTVSMDDSCAAMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWGRKIQNEKFRWHNSQWLEMVE----NRQMDEAE--PFMYGDEGEPF----LQGGDILERPDLF----YSADGLITADSAISPEFFTDFQNGDFVGPPGFPSSAVSDGFGQVAGAPV-RPTTAYGFRPD 778          
BLAST of Kinesin-associated protein 3 vs. Ensembl Cavefish
Match: kifap3a (kinesin associated protein 3 [Source:NCBI gene;Acc:103028328])

HSP 1 Score: 764.992 bits (1974), Expect = 0.000e+0
Identity = 411/768 (53.52%), Postives = 561/768 (73.05%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQ---------KDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLY-DTGMNYPYNTVGADDILSRYEMLMSGMYDENGL-PVDGSVSP--VLDPSLGSFTDEFGRP 2345
             DDVRYL + ++ G++DVHPTE+AL+VNYE++A I G++G+ M G+RK CQKIIR+K+L+ NT+I  LA+++V+ C+LI P+K+PEVE LL YL +RK  + +E K K   KP DP  F+  E+DE AN+N  D+Y+ELLYED+P+K++ + +IL LARNPDNLEEL  N T +GALARVLREDWK+S DLAT IIY+FFCFSSF  FH +I H+KIG L MNII+HELK+Y++W +ELQKK+   +         K+ +K LKK + +  KQEQL RV+ Y+LLN++ED   E+KMR KN+V +L KTL+R + ELL+L+VSFLKKLSI+ ENK+D+ + + VE+L+K VP  + DLL+++LRLL N+SFD+ LR ++V+ GLLPKL  L        L + ILY+IS +D++K  F  TDCIP +MKM+ +C  E++  E+++  INL  +   A +I EG GL+MLMKRA K +D ++MKM+RNISQH+G     F+DY+ DLA  I       + EF++EC+G L+NLTI DLD+E++L E+NLV ++K +L+P   EDDL+LEVV+++GTVS D++CA M+A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW  KIQ EKFRW+NSQWLEM+E      ++D AE   F+Y D G   P+   G  DIL R ++     Y  +GL   D ++SP    D   G F    G P
Sbjct:    3 ADDVRYLTRKVKGGSLDVHPTERALVVNYEVEARILGQMGDAMLGERKACQKIIRLKSLNPNTDIGSLARKVVEECRLISPTKLPEVEQLLFYLQNRK--SPTEKKEKKQIKPRDPTPFEGMELDEEANINSIDDYVELLYEDVPEKIRGATLILHLARNPDNLEELLQNETALGALARVLREDWKQSVDLATTIIYVFFCFSSFIQFHGLITHYKIGALCMNIIEHELKRYDLWQEELQKKKKAGEDDPENQNLKKEYEKALKKYQGLLTKQEQLLRVALYLLLNLSEDTRTELKMRNKNIVHLLVKTLDRDSEELLVLVVSFLKKLSIFLENKNDMAETDTVEKLAKLVPCDHEDLLNVTLRLLHNLSFDTGLRSKMVQVGLLPKLTALLGDEAQRHLSMRILYHISMDDRFKSMFAYTDCIPQVMKMLFDCGEERMDPELISFCINLAANKRNAQLICEGNGLKMLMKRALKLKDPLMMKMIRNISQHDGPSMNLFIDYVGDLAAQIGQNE---EEEFMIECLGTLANLTIPDLDWELVLKEYNLVPFLKDHLKPGCAEDDLVLEVVIMIGTVSMDDSCAAMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWGRKIQNEKFRWHNSQWLEMVENR----QMDEAE--PFMYGDEG--EPFLQGG--DILERPDLF----YSADGLITADSAISPEFFTDFQNGDFVGPPGFP 751          
BLAST of Kinesin-associated protein 3 vs. Ensembl Cavefish
Match: kifap3b (kinesin-associated protein 3b [Source:ZFIN;Acc:ZDB-GENE-030131-2219])

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 387/757 (51.12%), Postives = 530/757 (70.01%), Query Frame = 3
Query:   81 TDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASN-KPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKK-------RSEQ------QKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDP 2309
             D+ +YLK+ ++ G IDVHPTEKA++V+YE++A+I GE G  +  +RKECQKIIR+K+L+++T++  LA+++V+ CKLI PSK+ EVEHLL YL +RK+ +    ++K    KP +   F+  E+DE AN+N  DEY+ELLYE+IP+K++ S +IL LARNPDNLEEL  N T +GALARVLREDWK+S +LAT IIY+FFCFSSFS FH +I H+KIG L+MNII+HELK+Y++W DEL+KK       R E       +KD +K  KK +++  KQEQL RVS Y+LLN+AED   E+KMR KN+V +L KTL+R N ELL+L+VSFLKKLSI+ ENK+D+ + + VE+L++ +P  + DL++++LRLLLN+SFD+ LR ++V+ GLLPKL  L  S   + +V+CILY+IS +D+ K  F  TDCIP LMKM+ E   E++  E+++  INL  +   A +I EG GL+MLMKRA K +D ++MKM+RNISQH+G +K  FLDY+ DLA  I  +    D EFV+EC+G L+NLTI+DLD+E++L E+NLV ++K +L+P   EDDLILEVV+++GTVS D++CA M+A +GII +LIDL+                                  +APAY+IDLMHDKNTEIRKVCD TLDII+E+DEEW  KIQ EKFRW+NSQWLEM+E    + ++D  E   +LY    + P     AD             Y  +G+P +G +SP  DP
Sbjct:    3 ADEAQYLKRKVKPGNIDVHPTEKAIVVHYEVEASILGEKGAAVHEERKECQKIIRLKSLNASTDVSSLARKVVEECKLIHPSKLAEVEHLLFYLQNRKKPSSRGAENKNKLLKPRELTPFEGMELDEEANINKIDEYVELLYENIPEKIRGSTLILHLARNPDNLEELLQNETALGALARVLREDWKQSVELATTIIYVFFCFSSFSQFHGLITHYKIGALSMNIIEHELKRYDLWQDELEKKILLNNPLRDEDPDNLTLRKDHEKAYKKYQSLLVKQEQLLRVSLYLLLNLAEDTRTELKMRNKNIVHLLVKTLDRDNEELLVLVVSFLKKLSIFLENKNDMAEMDTVEKLARLIPCEHEDLMNVTLRLLLNLSFDTGLRCKMVQVGLLPKLTALLGSEGYKQIVMCILYHISIDDRSKAMFAYTDCIPQLMKMLFEHGEERIDSELISFCINLAANKRNAHIICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPLKQLFLDYVGDLAAQIGPEE---DEEFVIECLGTLANLTIADLDWELLLREYNLVPYLKDHLKPASAEDDLILEVVIMIGTVSMDDSCAVMLAKSGIIPALIDLL---------------------------------NEAPAYLIDLMHDKNTEIRKVCDNTLDIIAEYDEEWGRKIQSEKFRWHNSQWLEMVE----SRQMDEGE--PYLYG---DDPEGLEQAD-----------LFYSTDGMPPEGGLSP--DP 701          
BLAST of Kinesin-associated protein 3 vs. Ensembl Medaka
Match: kifap3b (kinesin associated protein 3 [Source:NCBI gene;Acc:101155384])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 398/802 (49.63%), Postives = 555/802 (69.20%), Query Frame = 3
Query:   78 MTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVS---EIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKR---------SEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEG-GANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGDFPY-----DRPDNRTGFRSD 2429
            M +D R LK+ +  G++DVHPTEKAL+V+YE+QA + G  G P+ G+RKE QKIIR+K+L   T++  LA+++V+ CKL+  S++P+VE LL+YL +RK   V    E K K   KP D   F+  E++E A++   DEY+ELLYE IP+K++ASA+ILQLARNPDNLEEL  N   +GALAR+LREDWK+S DLAT IIYIFFCFSSFS FH ++ H KIG L M+++DHE+K++++W +EL+KK             ++D DK LKK +++  KQE+L RVS Y+LLN+AED   E+KMR KN+V +L K LER + ELL+L+VSFLKKLSI+ ENK+D+ + + VE+L + VP  + DLL+++LRLL N+SFDS L+ ++V+ GLLPKL  L     N  +++ ILY+IS E++++  F  TDC+P LM+ + E   E + +E+++L INL  + + A ++ EG GL+MLMKRA K++  ++MKM+RNISQH+G  K+ F+D++ DLA  I  +  +   E+V+EC+G L+NLTI DLD+E++L E+NLV ++K  L+P   E DL LEVV+++GTVS D+ CA M+A +GII +LI+L+ A+QEDDE VCQIVYVFYQ++FH++TR +II++TQAPAY+IDLMHDKN EIRKVCD TLDII+E+DEEW  KIQ EKFR++N+QWLEM+E   A+ PE        +LYD                 R ++  S    +  +P D SVSP L      F  + G     ++  R  S  F        RP    GFR D
Sbjct:    1 MQEDTRCLKRKVTLGSLDVHPTEKALVVHYEVQAAVLGGGGGPVLGERKEGQKIIRLKSLSPTTDVAALAKKVVEECKLLPASRLPQVEQLLYYLQNRKSSPVEGKVERKEKRQVKPKDLTPFEGLELNEEASITKIDEYVELLYEGIPEKIRASALILQLARNPDNLEELLHNEAALGALARILREDWKQSVDLATIIIYIFFCFSSFSQFHAVVSHHKIGALCMSVLDHEIKRHDVWCEELRKKNKACESAPENGSLRRDQDKVLKKYQSLLTKQEELLRVSLYLLLNLAEDTRTELKMRNKNIVGLLVKLLERNDEELLVLVVSFLKKLSIFLENKTDMAEVDTVEKLGRLVPCEHEDLLNLTLRLLFNLSFDSGLKAKMVEVGLLPKLTALLGDENNRAVIMRILYHISMEERFRGMFAYTDCVPQLMQRLYEHGKEDMEVELISLLINLAANKKNAQLMCEGNGLKMLMKRALKTKACLLMKMIRNISQHDGPTKVLFIDFVGDLAAEIQAEEEE---EWVLECLGTLANLTIPDLDWELVLKEYNLVPYLKDQLKPGSAEGDLTLEVVIMIGTVSMDHACAVMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWGRKIQSEKFRFHNNQWLEMVESRQADEPE-------PYLYDN---------------ERTDLFYSA---DGIIPADVSVSPDL---FNPFQPQNGD----SHLTRDVSDVFDQTSSSPGRPATAYGFRPD 767          
BLAST of Kinesin-associated protein 3 vs. Planmine SMEST
Match: SMESG000038958.1 (SMESG000038958.1)

HSP 1 Score: 1649.41 bits (4270), Expect = 0.000e+0
Identity = 825/826 (99.88%), Postives = 825/826 (99.88%), Query Frame = 3
Query:   78 MTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIXXXXXXXXXXHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADXXXXXXXXXXNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSDMATIEHSMAVDKFGRPLIMDVNDEALQDYVGQYARKSRGDFR 2555
            MTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIFFCFSSFSDFHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADLLSISLRLLLNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKN EIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSDMATIEHSMAVDKFGRPLIMDVNDEALQDYVGQYARKSRGDFR
Sbjct:    1 MTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKECQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKEVAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLKASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIFFCFSSFSDFHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKDLDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSKTLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADLLSISLRLLLNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNISCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIASVISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAEVITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQPTKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEIVCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNAEIRKVCDWTLDIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYDTGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFTDEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSDMATIEHSMAVDKFGRPLIMDVNDEALQDYVGQYARKSRGDFR 826          
The following BLAST results are available for this feature:
BLAST of Kinesin-associated protein 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
KIFAP30.000e+054.26kinesin associated protein 3 [Source:HGNC Symbol;A... [more]
KIFAP30.000e+053.76kinesin associated protein 3 [Source:HGNC Symbol;A... [more]
KIFAP30.000e+052.99kinesin associated protein 3 [Source:HGNC Symbol;A... [more]
KIFAP30.000e+053.88kinesin associated protein 3 [Source:HGNC Symbol;A... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
kap-11.211e-11034.69Kinesin associated protein kap-1; Kinesin-Associat... [more]
kap-12.365e-10433.67Kinesin associated protein kap-1; Kinesin-Associat... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Kap33.342e-11944.72gene:FBgn0028421 transcript:FBtr0289954[more]
Kap33.412e-11944.72gene:FBgn0028421 transcript:FBtr0289956[more]
Kap31.794e-11844.72gene:FBgn0028421 transcript:FBtr0343145[more]
Kap32.137e-11844.72gene:FBgn0028421 transcript:FBtr0289955[more]
Kap32.453e-11844.72gene:FBgn0028421 transcript:FBtr0343146[more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
kifap3a0.000e+052.90kinesin-associated protein 3a [Source:ZFIN;Acc:ZDB... [more]
kifap3b0.000e+054.25kinesin-associated protein 3b [Source:ZFIN;Acc:ZDB... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kifap30.000e+054.59kinesin-associated protein 3 [Source:NCBI gene;Acc... [more]
kifap30.000e+054.93kinesin-associated protein 3 [Source:NCBI gene;Acc... [more]
kifap30.000e+054.51kinesin-associated protein 3 [Source:NCBI gene;Acc... [more]
kifap30.000e+055.12kinesin-associated protein 3 [Source:NCBI gene;Acc... [more]
kifap30.000e+055.15kinesin-associated protein 3 [Source:NCBI gene;Acc... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Kifap30.000e+054.26kinesin-associated protein 3 [Source:MGI Symbol;Ac... [more]
Kifap30.000e+056.95kinesin-associated protein 3 [Source:MGI Symbol;Ac... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q26626|KIFA3_STRPU0.000e+053.50Kinesin-associated protein 3 OS=Strongylocentrotus... [more]
sp|Q92845|KIFA3_HUMAN0.000e+054.26Kinesin-associated protein 3 OS=Homo sapiens OX=96... [more]
sp|P70188|KIFA3_MOUSE0.000e+054.26Kinesin-associated protein 3 OS=Mus musculus OX=10... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210R5E30.000e+055.06Kinesin-associated protein 3 OS=Mizuhopecten yesso... [more]
A0A2R2MLN00.000e+055.19kinesin-associated protein 3-like OS=Lingula ungui... [more]
A0A267DQM30.000e+052.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GUR20.000e+052.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I5H20.000e+052.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
kifap3a0.000e+052.26kinesin associated protein 3 [Source:NCBI gene;Acc... [more]
kifap3a0.000e+053.52kinesin associated protein 3 [Source:NCBI gene;Acc... [more]
kifap3b0.000e+051.12kinesin-associated protein 3b [Source:ZFIN;Acc:ZDB... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Kinesin-associated protein 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
kifap3b0.000e+049.63kinesin associated protein 3 [Source:NCBI gene;Acc... [more]
back to top
BLAST of Kinesin-associated protein 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000038958.10.000e+099.88SMESG000038958.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008904 ID=SMED30008904|Name=Kinesin-associated protein 3|organism=Schmidtea mediterranea sexual|type=transcript|length=2635bp
TAATTTAATATTCATTTTGTTTATGAGTTGTCTTCATGGTGATAAATTCT
TTTCTCTTGAAATAAAATTTTTAAAATATGACTGATGACGTCAGATATTT
AAAAAAAAATATAAGAACTGGAACTATTGATGTGCATCCAACTGAAAAAG
CATTAATAGTAAATTATGAATTACAAGCTACTATTTTCGGAGAACTTGGC
AATCCTATGGCAGGGGATAGAAAAGAATGTCAAAAAATAATTCGTGTGAA
AAATCTTGACAGCAATACAAATATTCCAAAATTAGCCCAAGAAATAGTCG
ATAGTTGTAAATTAATACAACCATCCAAAATTCCAGAAGTTGAGCATTTA
TTACATTATTTATTAAGTCGTAAAGAAGTAGCAGTTTCAGAAATCAAATC
AAAAGCAAGTAATAAGCCAGCTGATCCCGGAGCATTTGATTCAACTGAAA
TTGATGAAATCGCAAATCTCAATGACACAGATGAATATCTGGAGCTACTT
TATGAAGATATTCCAGACAAATTAAAAGCATCTGCAATGATTTTGCAACT
TGCTCGAAATCCTGATAATTTAGAGGAACTATATGATAACAGTACCCTTA
TTGGAGCATTGGCACGAGTTCTTCGAGAAGATTGGAAAGAAAGCACAGAT
TTAGCCACAAATATTATTTACATATTTTTTTGTTTTTCATCATTTTCCGA
TTTTCATCAAATCATAATCCATTTCAAAATTGGCACATTGACGATGAACA
TCATAGATCACGAATTAAAGAAGTACGAAATGTGGATTGACGAATTGCAG
AAAAAACGTTCAGAGCAACAAAAAGATCTTGATAAAACATTAAAGAAGGT
CGAAACAATGACGCGCAAACAAGAACAATTGTTTAGAGTATCTTACTACA
TGCTTCTCAATGTCGCGGAAGATATAAGTCTAGAAATAAAGATGCGCACT
AAGAACTTAGTCGAAATGCTGTCTAAAACTCTGGAAAGAAAGAATCCTGA
ACTTTTGATTTTGATCGTTTCCTTTTTAAAAAAGTTGAGTATTTACGGCG
AGAATAAATCTGATTTGGTTAAATACAATGTAGTTGAGCGACTTTCTAAG
CACGTTCCTAATTCAAATGCTGATTTGTTAAGCATTTCATTGCGATTGTT
ATTAAATATGAGCTTTGATTCTGCTTTGCGTTTGCGAATCGTCAAATGTG
GTTTGTTACCAAAATTGGTCAATTTATCTCAATCGCTAGAAAATGAAACT
TTAGTATTGTGTATTCTTTATAATATCAGTTGTGAAGATAAGTATAAACC
TTATTTTATGAAAACTGATTGCATTCCTTACTTAATGAAAATGATTCTCG
AGTGCAAAATGGAGAAAGTTCCAATTGAAATTGTTGCTCTTGCTATCAAT
TTAGTTTGTGATACAGAGATTGCGTCGGTTATCAGTGAAGGAAAAGGGTT
ACGTATGTTAATGAAGCGAGCATATAAATCTAGGGATTTCATGATAATGA
AAATGCTCCGTAATATTTCTCAGCATGAAGGAGAAATTAAAATGAGGTTT
TTGGATTACATCAGTGACTTAGCTGAAGTAATTACCAATAAGTCTGGCAA
ATTCGACCATGAATTTGTCGTTGAGTGCGTTGGAATTTTGAGCAATCTGA
CAATATCTGATCTCGACTTTGAAATGATTTTGACTGAGTTTAATTTAGTG
GACTGGATGAAGCAAAATTTACAACCGACTAAAATTGAAGATGATTTAAT
TTTGGAAGTAGTAATGCTTGTTGGTACAGTATCCAATGATAATACATGTG
CAAAAATGATTGCTGATGCTGGAATTATTCAAAGTCTGATAGATCTAATA
ACAGCTAAACAAGAAGATGATGAAATTGTTTGTCAGATTGTTTATGTTTT
CTATCAATTAATTTTCCATGAATCCACTCGAAAAATAATCATCGAAAAAA
CTCAAGCACCGGCATATATAATCGACTTGATGCATGACAAGAATACTGAA
ATCCGCAAAGTCTGTGATTGGACATTAGATATCATATCTGAATTTGATGA
AGAATGGTCAAATAAAATTCAACAAGAAAAGTTTCGTTGGTATAATAGCC
AGTGGTTGGAAATGATTGAAGGCGGTGCAAACACTCCAGAACTAGATCCC
GCCGAAGATCCTGCGTTTCTTTATGACACAGGAATGAATTATCCCTATAA
TACAGTGGGAGCAGATGATATACTTAGTAGATATGAAATGTTAATGTCCG
GAATGTATGACGAAAACGGTTTACCAGTCGATGGATCAGTAAGTCCAGTA
TTGGATCCTTCTCTGGGATCATTTACAGATGAATTTGGTCGGCCGGTGCA
ATTGGCAAATTATATGCGTTTTGCATCTGGTGACTTTCCCTACGATAGGC
CTGATAATCGAACAGGCTTCCGGTCTGACATGGCAACGATTGAACATTCT
ATGGCTGTAGATAAATTTGGTCGACCCTTGATAATGGATGTTAATGATGA
AGCACTGCAAGACTATGTCGGCCAATATGCTAGAAAGTCAAGAGGAGATT
TCAGATAGAAAAATAATTGATGTTTAACAATAAATAAATTCTTTAATTTG
TTTTATTTTTAATTTGAAAATGGTTTAATAAAAAA
back to top

protein sequence of SMED30008904-orf-1

>SMED30008904-orf-1 ID=SMED30008904-orf-1|Name=SMED30008904-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=827bp
MTDDVRYLKKNIRTGTIDVHPTEKALIVNYELQATIFGELGNPMAGDRKE
CQKIIRVKNLDSNTNIPKLAQEIVDSCKLIQPSKIPEVEHLLHYLLSRKE
VAVSEIKSKASNKPADPGAFDSTEIDEIANLNDTDEYLELLYEDIPDKLK
ASAMILQLARNPDNLEELYDNSTLIGALARVLREDWKESTDLATNIIYIF
FCFSSFSDFHQIIIHFKIGTLTMNIIDHELKKYEMWIDELQKKRSEQQKD
LDKTLKKVETMTRKQEQLFRVSYYMLLNVAEDISLEIKMRTKNLVEMLSK
TLERKNPELLILIVSFLKKLSIYGENKSDLVKYNVVERLSKHVPNSNADL
LSISLRLLLNMSFDSALRLRIVKCGLLPKLVNLSQSLENETLVLCILYNI
SCEDKYKPYFMKTDCIPYLMKMILECKMEKVPIEIVALAINLVCDTEIAS
VISEGKGLRMLMKRAYKSRDFMIMKMLRNISQHEGEIKMRFLDYISDLAE
VITNKSGKFDHEFVVECVGILSNLTISDLDFEMILTEFNLVDWMKQNLQP
TKIEDDLILEVVMLVGTVSNDNTCAKMIADAGIIQSLIDLITAKQEDDEI
VCQIVYVFYQLIFHESTRKIIIEKTQAPAYIIDLMHDKNTEIRKVCDWTL
DIISEFDEEWSNKIQQEKFRWYNSQWLEMIEGGANTPELDPAEDPAFLYD
TGMNYPYNTVGADDILSRYEMLMSGMYDENGLPVDGSVSPVLDPSLGSFT
DEFGRPVQLANYMRFASGDFPYDRPDNRTGFRSDMATIEHSMAVDKFGRP
LIMDVNDEALQDYVGQYARKSRGDFR*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000231vitelline gland
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR008658KAP3
IPR000225Armadillo
IPR011989ARM-like
IPR016024ARM-type_fold
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005488binding
GO:0019894kinesin binding
Vocabulary: cellular component
TermDefinition
GO:0005871kinesin complex
GO:0016939kinesin II complex
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 230..257
NoneNo IPR availableSMARTSM01297KAP_2coord: 12..722
e-value: 0.0
score: 1344.1
NoneNo IPR availablePFAMPF05804KAPcoord: 13..682
e-value: 4.9E-297
score: 986.9
IPR000225ArmadilloSMARTSM00185arm_5coord: 571..613
e-value: 0.0055
score: 25.9
coord: 323..363
e-value: 24.0
score: 10.4
coord: 364..402
e-value: 19.0
score: 11.2
coord: 615..655
e-value: 43.0
score: 8.4
coord: 478..526
e-value: 450.0
score: 0.4
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 275..669
e-value: 4.2E-28
score: 100.1
IPR008658Kinesin-associated protein 3PANTHERPTHR15605KINESIN-ASSOCIATED PROTEINScoord: 9..688
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 272..660