Calcium binding egf domain-containing protein

Overview
NameCalcium binding egf domain-containing protein
Smed IDSMED30008893
Length (bp)5111
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium binding egf domain-containing protein (SMED30008893) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium binding egf domain-containing protein (SMED30008893) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium binding egf domain-containing protein (SMED30008893) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium binding egf domain-containing protein (SMED30008893) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008893

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal cellSMED30008893h1SMcG0020157 dd_Smed_v4_1748_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
prepharyngeal regionSMED30008893h1SMcG0020157 SmedASXL_004874SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30008893h1SMcG0020157 SmedASXL_004874SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30008893h1SMcG0020157 SmedASXL_004874SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30008893h1SMcG0020157 dd_Smed_v4_1748_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008893h1SMcG0020157 dd_Smed_v4_1748_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30008893h1SMcG0020157 dd_Smed_v6_1748_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30008893h1SMcG0005357 OX_Smed_1.0.04236ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30008893h1SMcG0020157 OX_Smed_1.0.14759ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30008893h1SMcG0020157 OX_Smed_1.0.04236ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30008893h1SMcG0020157 dd_Smed_v6_1748_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig8392uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig8392newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig12743newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig12743uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig40248uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig40248newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig16349newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30008893h1SMcG0020157 Contig16349uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Match: FBN3 (fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794])

HSP 1 Score: 157.532 bits (397), Expect = 1.182e-37
Identity = 101/274 (36.86%), Postives = 141/274 (51.46%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C D++EC S    C  N+ CIN PGS+ C C+   + L+P   C  +  EC   R+I ++C      S  +C  + G   C+C RGF     +  C+DI+EC+  PC    TC+N  GS++C C  GF  + +  CVD DEC     ++C   G C N  GS+ C C +G+E   D K C+D +EC +    C      CQN  GSF+C+CP G + +   C+DIDEC +        TC NS G F C C  GFV +++   C
Sbjct: 1793 GFNYNSILLACEDVDECGSRESPCQQNADCINIPGSYRCKCTRGYK-LSPGGACVGR-NEC---REIPNVC------SHGDCMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQPC-GNGTCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCR-FGHCLNTAGSFHCLCQDGFELTADGKNCVDTNECLSLAGTCLPGT--CQNLEGSFRCICPPGFQVQSDHCIDIDECSEEPNLCLFGTCTNSPGSFQCLCPPGFVLSDNGHRC 2051          

HSP 2 Score: 145.206 bits (365), Expect = 7.394e-34
Identity = 112/305 (36.72%), Postives = 139/305 (45.57%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----------DAVTCRSPA-------NCSSSNGVEFC-VCPRGFTYDLKEGCI------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ ++ G  C D+NEC+S      N RC+NT GSF C C            C     E  F R     C          D   C   A        C     +EF  +CPRG  +  ++         D+NEC + P L    TCRN+ GSF C C  GF        C DIDECR S    G G C N  GS+ C C  GYE  ++  K C+D+DEC     LC+G    C NT GS+KC CP G E   KG  C DIDEC   +  CP G  C+N  G F C+C+ GF    D + C
Sbjct:  894 DPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCFLRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEACPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALDAQERNCTDIDECRISPDLCGQGTCVNTPGSFECECFPGYESGFMLMKNCMDVDECARDPLLCRGGT--CTNTDGSYKCQCPPGHELTAKGTACEDIDECSLSDGLCPHGQ-CVNVIGAFQCSCHAGFQSTPDRQGC 1195          

HSP 3 Score: 127.872 bits (320), Expect = 1.407e-28
Identity = 94/281 (33.45%), Postives = 136/281 (48.40%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC--QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+Q   D   C+DINEC       +  CINT GS+ C C      +     CA  ++EC    ++   CD        +C++  G   C+C  GF  T D++  C+D++EC++NP  CL    C N+ GSF C C  G+       GC D+DEC      C  +  C N+ GS+ C C  G+   +   C D+DEC + +  C  +GD   C N PGS++C C  G       C D DEC +N    +   C+N+ GG+ C C  GF    D + C+
Sbjct: 1181 SCHAGFQSTPDRQGCVDINECRVQNGGCDVHCINTEGSYRCSCGQGYSLMPDGRACA-DVDECEENPRV---CDQ------GHCTNMPGGHRCLCYDGFMATPDMRT-CVDVDECDLNPHICL-HGDCENTKGSFVCHCQLGYMVRKGATGCSDVDECEVGGHNCDSHASCLNIPGSFSCRCLPGW-VGDGFECHDLDECVSQEHRCSPRGD---CLNVPGSYRCTCRQGFAGDGFFCEDRDECAENVDLCDNGQCLNAPGGYRCECEMGFDPTEDHRACQ 1445          

HSP 4 Score: 124.79 bits (312), Expect = 1.322e-27
Identity = 91/270 (33.70%), Postives = 130/270 (48.15%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C  GY        C+D++EC  +       C NT GSF+C C     YL  E G   K ++EC+  +                C ++ G   C CP GFT    + C D +EC+  P  C     C N+ GSF C C +GF   S   GC D++EC     C     CQN +G YRC+C  G+ ++ +   C+D +EC  S   C   +  C+NT G F+CVCP G ++ +   GC ++DEC      P   +C N+ GGF C C +G+
Sbjct: 2431 HCQAGYTPDATATTCLDMDECSQVPKPCTFLCKNTKGSFLCSCPRG--YLLEEDGRTCKDLDECTSRQHNCQFL----------CVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG--CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTC--GSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRG-PCSYSCANTPGGFLCGCPQGY 2682          

HSP 5 Score: 122.479 bits (306), Expect = 6.225e-27
Identity = 90/275 (32.73%), Postives = 123/275 (44.73%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    D++EC  LA    +  CIN+ GSF C+C            C   ++ECS   K  +            C ++ G   C CPRG+  +     C D++EC       +  C N+ G+F C CP GF    H  C D DEC      C  +G C N  GS+RC CH G+  V    GC D++EC    +   G    CQN  G ++C CP G     +   CVD +EC  +      ++C N+ GGF C C  GF F+     C+
Sbjct: 2396 GSGYTAEGR---DVDECRMLAHLCAHGECINSLGSFRCHCQAGYTPDATATTCL-DMDECSQVPKPCTFL----------CKNTKGSFLCSCPRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG----CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQ 2651          

HSP 6 Score: 116.316 bits (290), Expect = 4.372e-25
Identity = 70/187 (37.43%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG---LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C +++G   C CP G++ D     C+D +EC++     + TC N  G F+C C  GF+  +   C DIDEC  +  +C+    C N  GSY C C  GY   ED   C D+DEC   ++ C    + C+N  G+F CVCP G   L    +GC D +EC  Q + C  G  C+N+ G F C C++GF
Sbjct: 2142 CVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFR--CHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGR-CVNTAGSFRCDCDEGF 2325          

HSP 7 Score: 114.39 bits (285), Expect = 1.924e-24
Identity = 72/184 (39.13%), Postives = 92/184 (50.00%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+T D++  CID++EC  +PC     C N  G++ C C  GFQ +     CVD+DEC  S      G C N  GS++C C+ G+E   D K C+D +EC  S     G   VC N  GSF C+C  G      G  C+DIDECQ    C  G  C N+ G F C C  G     D + C
Sbjct:  475 CECNVGYTQDVRGECIDVDECTSSPC-HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNG---VCLNEDGSFSCLCKPGFLLAPGGHYCMDIDECQTPGICVNGH-CTNTEGSFRCQCLGGLAVGTDGRVC 653          

HSP 8 Score: 105.145 bits (261), Expect = 1.026e-21
Identity = 96/299 (32.11%), Postives = 129/299 (43.14%), Query Frame = 3
Query: 2610 CIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD---AVTCRSPANCSSSN-------GVEFCVCP-------------RGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVV 3410
            C DI+EC + +       C NT G++ C C      +N    C    +   F R     C    A        C S N         E C  P              GF  D+  G  +DI+EC   P +     C N  GSF C CP GF Y S+   C D+DEC  R+S  C  N  C N+ GSYRC C  GY+      C+  +EC+    +C  GD   C +T GS+ C+C  G +    +  C+DIDEC +   C  G TC N  G +NC C  GFV  ++    + D+   +V
Sbjct: 1645 CEDIDECSTHSGICGPGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCF-RHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESP-CQQNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD---CMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQ-PCGNG-TCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLV 1936          

HSP 9 Score: 104.76 bits (260), Expect = 1.537e-21
Identity = 98/289 (33.91%), Postives = 126/289 (43.60%), Query Frame = 3
Query: 2607 NCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECRDSK---------ICS---GNG------IC-QNMIGSYRCACHNGYEYVE--DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C+D +EC +L         C+NT GSF C C +  E       C    E  S          A TC  P  C +  G   C+CP GF     + CIDI+EC+  P  CL   TC NS GSF C CP GF            + S  F   +  +C   K          CS   G G      +C Q    +++  C  G+  V   D    D++EC  +  +C   N VC NT GSF+C CP G  L++    CVD DEC   + C +G TC N  GGF C C  GF
Sbjct: 1925 DCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFELTADGKNCVDTNECLSL---------AGTCL-PGTCQNLEGSFRCICPPGFQVQ-SDHCIDIDECSEEPNLCL-FGTCTNSPGSFQCLCPPGFVLSDNGHRCFDTRQSFCFTRFEAGKCSVPKAFNTTKTRCCCSKRPGEGWGDPCELCPQEGSAAFQELCPFGHGAVPGPDDSREDVNECAENPGVCT--NGVCVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQG-TCTNVIGGFECACADGF 2198          

HSP 10 Score: 101.679 bits (252), Expect = 1.229e-20
Identity = 94/316 (29.75%), Postives = 129/316 (40.82%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK-EGCIDIN-ECNMNPCLDKATCRNSY------------------------------GSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            N G      G NC+D NEC +     N  C+N  GSF C C        P F  AP    C          D   C++P      +C+++ G   C C  G         C+D +        ++K +C   +                                F   C  G    +     DI+EC  D ++C+ NG+C+N+ GSYRC C+ GYE     K C D+DEC  +  LC  DN  CQN+PGS+ C CP G  + +    C D+DEC  ++ C  G  C N  G + C C  G   +    FC LD T
Sbjct:  559 NAGFELSPDGKNCVDHNECATSTMCVNGVCLNEDGSFSCLC-------KPGFLLAPGGHYC---------MDIDECQTPGICVNGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGL--GITTDGRDINECALDPEVCA-NGVCENLRGSYRCVCNLGYEAGASGKDCTDVDECALNSLLC--DNGWCQNSPGSYSCSCPPGFHFWQDTEICKDVDEC-LSSPCVSG-VCRNLAGSYTCKCGPGSRLDPSGTFC-LDST 850          

HSP 11 Score: 97.8265 bits (242), Expect = 1.726e-19
Identity = 73/214 (34.11%), Postives = 98/214 (45.79%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKI-CSGNGI-CQNMIGSYRCACHNGYEYV--EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            D+NEC ++     N  C+NT GSF C C            C    +ECS                   C++  G   C C  GF   L   C DI+EC++NP L    C N+ GS+ C CP G+        C D+DEC D +  C   G+ C+N+IG++ C C  G   +    +GC D +EC A   LC   N  C NT GSF+C C  G +
Sbjct: 2126 DVNECAENPGVCTNGVCVNTDGSFRCECPFGYSLDFTGINCV-DTDECSVGHPC----------GQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCV--NGRCVNTAGSFRCDCDEGFQ 2326          

HSP 12 Score: 97.8265 bits (242), Expect = 2.025e-19
Identity = 62/168 (36.90%), Postives = 84/168 (50.00%), Query Frame = 3
Query: 2889 IDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            IDI     N CL+   C  +  S+ C C  G+   V   C+D+DEC  S    G+  C N+ G+Y C C+ G++    +  C+D+DEC  S  LC      C NT GSF+CVC  G E    G  CVD +EC  +  C  G  C+N  G F+C C  GF+      +C
Sbjct:  451 IDICRHFTNLCLN-GRCLPTPSSYRCECNVGYTQDVRGECIDVDECTSSPCHHGD--CVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGR--CVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNG-VCLNEDGSFSCLCKPGFLLAPGGHYC 612          

HSP 13 Score: 97.0561 bits (240), Expect = 3.299e-19
Identity = 92/305 (30.16%), Postives = 121/305 (39.67%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEEC-SFERKIIS--ICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY Q +   CID++EC S  CH+   C+N PG++ C C        P F   P  + C   +  I+S  +C          C ++ G   CVC  GF      + C+D NEC  +       C N  GSF C C  GF  +   H+ C+DIDEC+   IC  NG C N  GS+RC C  G                   Y  +E   C        +K  C       G    CQ  P      F+ +C  GL     G  DI+EC  +        C N  G + C CN G+      K C
Sbjct:  477 CNVGYTQDVRGECIDVDECTSSPCHHGD-CVNIPGTYHCRCY-------PGFQATPTRQACVDVDECIVSGGLCHL------GRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNGVCLNEDGSFSCLCKPGFLLAPGGHY-CMDIDECQTPGICV-NGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG-RDINECALDPEVCANGVCENLRGSYRCVCNLGYEAGASGKDC 764          

HSP 14 Score: 94.3597 bits (233), Expect = 1.790e-18
Identity = 92/309 (29.77%), Postives = 129/309 (41.75%), Query Frame = 3
Query: 2580 GKGYQ--KIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDI--------------NECNMNPCLDKATCRNSYGS----------------FDCFC---PKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G+G+Q  +I     DI+EC  L        C+NT GSF C C     +L+        I+ECS    I           P  C ++ G   CVCP  +   +    C+D+              NE   N       C  + G                 +   C     GF   +H G  +DIDEC +      NGIC N IGS+RC C  G+ Y      C D+DEC + +  CQ  N  C N PGS++C C  G +    G CV  +EC++  N C  G  C+++ G + C C++GF  + D   C
Sbjct: 1591 GEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGY-HLSEHTRICEDIDECSTHSGICG---------PGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESPCQ-QNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD-CMDTEGSYMCLCHRGFQASADQTLC 1887          

HSP 15 Score: 94.3597 bits (233), Expect = 2.065e-18
Identity = 102/334 (30.54%), Postives = 135/334 (40.42%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKI-------------ISICDAVTCRSPAN--------CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDE----------CQ------ASKKLC-------QGDNVVCQNTPG----SFKCVC----PVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+  + G NC DINEC       N  CINTPGS++C C  + E LNP   GC   +   C  E                + +  A  C S           C  +N  E+  +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  S H   C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +          CQ       ++K+C       Q  N  C+  P      ++ +C    P  L     G  +DIDEC +         CIN  G F C C  GF +N+    CE
Sbjct: 1472 CNGGYELDRGGGNCTDINECADPVNCINGVCINTPGSYLCSCPQDFE-LNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICEDIDECSTHSGICGPGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACE 1804          

HSP 16 Score: 92.0485 bits (227), Expect = 1.056e-17
Identity = 88/297 (29.63%), Postives = 123/297 (41.41%), Query Frame = 3
Query: 2610 CIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-------------SVHFGCVDIDEC--------RDSKICSGNG------ICQ----NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C+D++EC      CH   RC+NT GSF C C       N  F  +P  + C    +    C   T      C + +G   C+C  GF   L  G   C+DI+EC          C N+ GSF C C  G                S  +G ++   C          S+ C  N        CQ         ++  C +G     D    DI+EC    ++C   N VC+N  GS++CVC +G E    G  C D+DEC  N+   +   C NS G ++C+C  GF F  DT+ C+
Sbjct:  529 CVDVDECIVSGGLCHL-GRCVNTEGSFQCVC-------NAGFELSPDGKNCVDHNE----CATSTMCVNGVCLNEDGSFSCLCKPGFL--LAPGGHYCMDIDECQTPGICVNGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDGR--DINECALDPEVCA--NGVCENLRGSYRCVCNLGYEAGASGKDCTDVDECALNSLLCDNGWCQNSPGSYSCSCPPGFHFWQDTEICK 807          

HSP 17 Score: 91.6633 bits (226), Expect = 1.454e-17
Identity = 84/310 (27.10%), Postives = 126/310 (40.65%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID---------------------------IDECQASKKLCQGDNVVCQNTPGS----FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G  C D++EC S    C     C+N PGS+ C C   + +    F C  + +EC+   + + +CD         C ++ G   C C  GF   +    C D++EC         +C N  G F C C  G++     G C DI+EC D   C  NG+C N  GSY C+C   +E      GC+D                           +        L +     C+  P +    ++ +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  +  T+ CE
Sbjct: 1350 CLPGWVGDGFECHDLDECVSQEHRCSPRGDCLNVPGSYRCTC--RQGFAGDGFFCEDR-DECA---ENVDLCDN------GQCLNAPGGYRCECEMGFDPTEDHRACQDVDECAQGNLCAFGSCENLPGMFRCICNGGYELDRGGGNCTDINECADPVNCI-NGVCINTPGSYLCSCPQDFELNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICE 1646          

HSP 18 Score: 83.5741 bits (205), Expect = 4.252e-15
Identity = 63/198 (31.82%), Postives = 87/198 (43.94%), Query Frame = 3
Query: 2559 TCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNP-CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            TC+   C  G+ +    C D +EC +    C  +  C NTPGSF C C      ++   GC   + EC               R    C +  G   C CP+GFT +     C+D NEC ++P     A+CRN+ G F C CP GF +    G C ++DEC   +  CS +  C N  G + C C  GY       C+
Sbjct: 2511 TCR---CPPGFTQHHQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCE-DVNECDGPH-----------RCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPCSYS--CANTPGGFLCGCPQGYFRAGQGHCV 2691          

HSP 19 Score: 81.6481 bits (200), Expect = 1.647e-14
Identity = 84/311 (27.01%), Postives = 126/311 (40.51%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINEC--NMNPCLDKAT-CRNSYGSFDCFCPKGFQ--YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDE----CQASKKLCQGDNVVCQNT----------------------PGS--FKCVCPVGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G NC+D +EC          C N  G F C C++  E           I+ECS    + +            C ++ G   C CP G+T  L+E    C D++EC      C  +   C+N  G+F C CP G +       GC D +EC        NG C N  GS+RC C  G++       C DI +     +  + +C+  +   +                        PG+  ++ +CP G  Y  +G  D+DEC+   + C  G  CINS G F C C  G+  +     C ++D+   V +P
Sbjct: 2163 GINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFE--PGLMMTCEDIDECSLNPLLCAF----------RCHNTEGSYLCTCPAGYT--LREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPGTSAYRKLCPHGSGYTAEG-RDVDECRMLAHLCAHGE-CINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKP 2457          

HSP 20 Score: 77.7962 bits (190), Expect = 2.215e-13
Identity = 89/312 (28.53%), Postives = 125/312 (40.06%), Query Frame = 3
Query: 2577 CGKGYQK-IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY--DLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDE-----------CRD------------------------SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G++  +   C DI+EC         RC NT GS++C C      L  +      ++EC+  ++    C A        C +  G   CVCP G        EGC D NEC+  P L     C N+ GSF C C +GFQ S     C DI +           CR                          ++C   G       +YR  C +G  Y  +    D+DEC+    LC      C N+ GSF+C C  G   +     C+D+DEC Q    P    C N+ G F C+C +G++   D + C+
Sbjct: 2194 CADGFEPGLMMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYT-LREDGAMCRDVDECADGQQD---CHA----RGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPG-----TSAYRKLCPHGSGYTAEGR--DVDECRMLAHLCAHGE--CINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPK-PCTFLCKNTKGSFLCSCPRGYLLEEDGRTCK 2487          

HSP 21 Score: 72.4034 bits (176), Expect = 9.748e-12
Identity = 60/215 (27.91%), Postives = 92/215 (42.79%), Query Frame = 3
Query: 2790 AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIG-----------SYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +V+C +   C  ++    C+C +G+T  +    I    C N   C+    C   YG     C + ++    FG V  + C+   + +     +C   +G           +    C  G+   +    C D+DECQA   LCQG +  C N  GSF C CPVG  L      C D++EC   +    G  C N+ G + CTC++GF  + D   C
Sbjct:  121 SVSCMNGGTCRGAS----CLCQKGYTGTVCGQPICDRGCHNGGRCIGPNRCACVYGFMGPQCERDYRTGPCFGQVGPEGCQHQLTGLVCTKALCCATVGRAWGLPCELCPAQPHPCRRGFIPNIHTGACQDVDECQAVPGLCQGGS--CVNMVGSFHCRCPVGHRLSDSSAACEDVNECLSLSGLCSGGDCTNTVGSYVCTCSQGFASSLDGTHC 329          

HSP 22 Score: 67.0106 bits (162), Expect = 4.586e-10
Identity = 81/324 (25.00%), Postives = 125/324 (38.58%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRG-FTYDLKEGC--IDINECNMNPCLDKATCRN--------SYGS------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLCQGD-----NVVCQNTPG----SFKCVCPVGLEYKEKGCV-------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G +C D++EC  +SL C +N  C N+PGS+ C C     +      C   ++EC     +  +C  +       C   + ++    P G F  D  +G   + I E      L  A+ R+        ++GS       D  C +GF       C D++EC        NG C N  GS+RC C  G       + C+D+           DEC  +        +C         V C+  P      F  +CP GL +  +  +       D++EC+         TC N+ G F+C C  GF  +   + C
Sbjct:  751 CNLGYEAGASGKDCTDVDECALNSLLC-DNGWCQNSPGSYSCSCPPGFHFWQDTEICK-DVDECLSSPCVSGVCRNLAGSYTCKCGPGSRLD----PSGTFCLDSTKGTCWLKIQESRCEVNLQGASLRSECCATLGAAWGSPCERCEIDPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCFLRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEACPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALDAQERNC 1068          

HSP 23 Score: 58.5362 bits (140), Expect = 1.454e-7
Identity = 59/179 (32.96%), Postives = 77/179 (43.02%), Query Frame = 3
Query: 2628 CDSLACHNNSRCINTPGSFVCYC--------SNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP-------RGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGY 3107
            CD   CHN  RCI   G   C C           +    P FG     E C  +   +    A+ C   A    + G+   +CP       RGF  ++  G C D++EC   P L    +C N  GSF C CP G + S     C D++EC   S +CSG G C N +GSY C C  G+
Sbjct:  151 CDR-GCHNGGRCI---GPNRCACVYGFMGPQCERDYRTGPCFGQVGP-EGCQHQLTGLVCTKALCC---ATVGRAWGLPCELCPAQPHPCRRGFIPNIHTGACQDVDECQAVPGLCQGGSCVNMVGSFHCRCPVGHRLSDSSAACEDVNECLSLSGLCSG-GDCTNTVGSYVCTCSQGF 320          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Match: FBN2 (fibrillin 2 [Source:HGNC Symbol;Acc:HGNC:3604])

HSP 1 Score: 146.747 bits (369), Expect = 2.707e-34
Identity = 106/266 (39.85%), Postives = 150/266 (56.39%), Query Frame = 3
Query: 2610 CIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC   D+L C  N+ CIN+PGS+ C C+   + L+P   C  +  EC    +I ++C      S   C    G   C+C  GF     +  C+D++EC  +PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N IGS++C C+ GYE   D K CID +EC A    C      CQN  GSF+C+CP G E K + C+DI+EC ++ N C  GS C N+ GGF C C  GFV +++ + C
Sbjct: 1848 CEDIDECSNGDNL-CQRNADCINSPGSYRCECAAGFK-LSPNGACVDR-NEC---LEIPNVC------SHGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERHPC-GNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCR-NGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPG--TCQNLEGSFRCICPPGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCPPGFVLSDNGRRC 2096          

HSP 2 Score: 122.479 bits (306), Expect = 6.748e-27
Identity = 106/305 (34.75%), Postives = 143/305 (46.89%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSF---ERKIISIC------DAVTCRSPA-------NCSSSNGVEF-CVCPRGFTYDLKEGCI-------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G  +I G  C D+NEC+       N RC+N+ GSF C C            C   ++E+C     E + I         DA  C   A        C      E+  +CPRG  +  +   +       DINEC   P +     CRN+ GSF C C  GF   +    C DIDECR S    G+GIC N  GS+ C C  GYE  ++  K C+DIDEC+ +  LC+G    C NT GSF+C CP+G E     + CVDI+EC  ++       C+N  G + C+CN G+    D + C
Sbjct:  938 DTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCTDIDECRISPDLCGSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGG--TCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGC 1240          

HSP 3 Score: 121.324 bits (303), Expect = 1.380e-26
Identity = 100/281 (35.59%), Postives = 139/281 (49.47%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH---FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC  +    +++C N+ GS+ C CS     +     CA  I+EC        ICD         C++  G   C+C  GF  + D+K  CID+NEC++N   C+    C N+ GSF C C  G  YSV     GC D+DEC   +  C  +  C N+ GS++C+C  G+     K CID+DEC      C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N  G + C C  GF   +D++ C+
Sbjct: 1226 SCNPGYQATPDRQGCTDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCA-DIDEC---ENNPDICDG------GQCTNIPGEYRCLCYDGFMASMDMKT-CIDVNECDLNSNICM-FGECENTKGSFICHCQLG--YSVKKGTTGCTDVDECEIGAHNCDMHASCLNIPGSFKCSCREGWIGNGIK-CIDLDECSNGTHQCS-INAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENINLCENGQCLNVPGAYRCECEMGFTPASDSRSCQ 1490          

HSP 4 Score: 120.168 bits (300), Expect = 3.046e-26
Identity = 96/264 (36.36%), Postives = 129/264 (48.86%), Query Frame = 3
Query: 2616 DINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC +        +C NT GSF C C++       E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  TC N+ GSF C CP G + S     CVDI+EC  S     NG C NMIG+Y+C+C+ GY+   D+ GC DIDEC      C   +  C N+ GS++C C  G      G  C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1073 DINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCT-DIDECRISPDLC---------GSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCTDIDECMIMNGGC---DTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTC 1323          

HSP 5 Score: 113.62 bits (283), Expect = 2.823e-24
Identity = 74/184 (40.22%), Postives = 94/184 (51.09%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D    CID++EC  NPC +   C N+ GS+ C C  GFQ +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC  +     G   +C N  GSFKC+C  G      G  C D+DECQ    C  G  CINS G F C C  G     D + C
Sbjct:  519 CECNMGYKQDANGDCIDVDECTSNPCTN-GDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNCVDHDECTTTNMCLNG---MCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGH-CINSEGSFRCDCPPGLAVGMDGRVC 697          

HSP 6 Score: 113.62 bits (283), Expect = 3.230e-24
Identity = 98/295 (33.22%), Postives = 133/295 (45.08%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G   +  G+ C+D NEC +      N RC+N  GS+ C C+   +  +    C    +   F   + +IC   +       +S   C    G     E C          +CP G  Y       DI+EC + P L     C N+ GSF CFC  G+   +    C+D+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  Q + C     C N+ G F+C C +GF  +     CE
Sbjct: 2326 GMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDG--RDIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPKPC--NYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQ---FLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE 2613          

HSP 7 Score: 111.694 bits (278), Expect = 1.094e-23
Identity = 93/277 (33.57%), Postives = 124/277 (44.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DI+EC  +     N +CINT GSF C+C            C   ++ECS   K            P N  C ++ G   C CPRG+   +  + C D++EC       +  C N+ G F C CP GF    H  C+D +EC     +C   GICQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC   N C   S C N+ G + C C  GF F+  +  C 
Sbjct: 2440 GPGYTTDGR---DIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCI-DLDECSQSPK------------PCNYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG----CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSAS-CYNTLGSYKCACPSGFSFDQFSSACH 2694          

HSP 8 Score: 106.686 bits (265), Expect = 4.048e-22
Identity = 88/259 (33.98%), Postives = 122/259 (47.10%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            G +CID++EC       N  C NT GS+ C C     Y+  E G   K ++EC  ++                C ++ G   C CP GFT      CID NEC   P  C  K  C+N+ GSF C C +GF   +    C D+DEC  +  C     CQN++G YRC C  GY ++ +   C+D +EC          +  C NT GS+KC CP G  + +    C D++EC  +   P    C N+ GG+ C C  G+
Sbjct: 2486 GTSCIDLDECSQSPKPCNYICKNTEGSYQCSCPRG--YVLQEDGKTCKDLDECQTKQHNCQFL----------CVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG--CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNAC---GSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKN-PCNYGCSNTEGGYLCGCPPGY 2725          

HSP 9 Score: 102.064 bits (253), Expect = 8.589e-21
Identity = 101/317 (31.86%), Postives = 139/317 (43.85%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK--------------------------EGCIDINEC-----------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G++    G NC+D +EC +     N  CIN  GSF C C        P F  AP    C+         D   C++P      +C +S G   C CP G    +                            G +  +EC              PC  K +       F   C  G   +V     DI+EC  D  IC+ NGIC+N+ GSYRC C++GYE     + CIDIDEC  ++ LC  DN +C+NTPGS+ C CP G  ++   + C DI+EC ++N C  G+ C N+ G FNC C+ G   ++    C
Sbjct:  602 CNAGFELTTDGKNCVDHDECTTTNMCLNGMCINEDGSFKCICK-------PGFVLAPNGRYCT---------DVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNS-----AEFHGLCSSGVGITVDG--RDINECALDPDICA-NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLC--DNGLCRNTPGSYSCTCPPGYVFRTETETCEDINEC-ESNPCVNGA-CRNNLGSFNCECSPGSKLSSTGLIC 890          

HSP 10 Score: 98.2117 bits (243), Expect = 1.312e-19
Identity = 66/185 (35.68%), Postives = 101/185 (54.59%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GFT+D+  G  +DI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC +   +C  N  C N  GSYRC C  G++   +  C+D +EC     +C   + +C +  GS++C+C  G +  +    C+D+DEC+++  C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1796 GFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCS--HGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERH-PCGNG-TCKNTVGSYNCLCYPGFELTHNNDCLDIDE 1976          

HSP 11 Score: 95.9005 bits (237), Expect = 7.301e-19
Identity = 94/311 (30.23%), Postives = 137/311 (44.05%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDEC--------------------QASKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C +G+   G  CID++EC   +  C  N++C+NTPGS+ C CS  E +    F C+  ++EC+   + I++C+         C +  G   C C  GFT       C DI+EC+        TC N  G F C C  G++     G C DIDEC D   C  NG+C N  G Y C C   ++      GC+D  +  C                      S+  C     +     C+  P      +  +CP G  ++         DIDECQ+     +G  CIN+FG F C C +G+  + DT+ CE
Sbjct: 1394 SCREGWIGNGIKCIDLDECSNGTHQCSINAQCVNTPGSYRCACS--EGFTGDGFTCS-DVDECA---ENINLCEN------GQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYELDRTGGNCTDIDECADPINCV-NGLCVNTPGRYECNCPPDFQLNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICE 1691          

HSP 12 Score: 91.2781 bits (225), Expect = 1.669e-17
Identity = 93/297 (31.31%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP----------------RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF------CP--------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        CINT GSF C C     YL+ +      I+EC     +           P  C ++ G   C+CP                + F Y    G    NE   N  + K  C  +Y     +      CP               GF + +H G  VDIDEC++      NG+C N IGS+RC C  G+ Y +    C DIDEC     LCQ  N  C N+PGS++C C  G +    G CVD +EC +  N C  G  C++  G + C C+ GF  + D   C
Sbjct: 1650 DIDECQELPGLCQGGNCINTFGSFQCECPQGY-YLSEDTRICEDIDECFAHPGVCG---------PGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFN--VTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQ-RNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHG-LCVDLQGSYQCICHNGFKASQDQTMC 1932          

HSP 13 Score: 87.0409 bits (214), Expect = 3.144e-16
Identity = 83/273 (30.40%), Postives = 118/273 (43.22%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNG-ICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            D+NEC +S    +N +CINT GSF C C            C    +ECS      +            C++  G   C C  GF       C DINEC  NP L    C N++GS++C CP G+        C D+DEC +    C   G +C+N+IG++ C C  G     D +GC+D +EC+    +C+  N  C N  GS++C C  G +    G   +D  Q           C   S+  N      C C+ G  + +  + C L  T
Sbjct: 2171 DVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCV-DTDECSIGNPCGN----------GTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICE--NGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGT 2430          

HSP 14 Score: 83.1889 bits (204), Expect = 4.411e-15
Identity = 97/323 (30.03%), Postives = 135/323 (41.80%), Query Frame = 3
Query: 2568 DVNCGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGYEYVED-------------KGCI----------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPK 3419
            + NC +G++     NC DINEC         RC+NT GS+ C C         +  C   ++EC+   + +  C++        C +  G   C+CP G       EGC+D NEC   P + +   C N  GS+ C C +GFQ S         EC D++           IC      +N++    C C  G  +                K C           DIDEC+    LC   N  C NT GSF+C C VG      G  C+D+DEC Q+   P    C N+ G + C+C +G+V   D K C   K L+  Q K
Sbjct: 2236 ECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCK-DLDECA---EGLHDCESRG----MMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSG-----TECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDGRDIDECKVMPNLCT--NGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPK-PCNYICKNTEGSYQCSCPRGYVLQEDGKTC---KDLDECQTK 2539          

HSP 15 Score: 82.8037 bits (203), Expect = 7.184e-15
Identity = 92/298 (30.87%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2604 DNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDI-------DECQ--------ASKKLCQ-GDNVV------------CQNTPG------------SFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            ++C+DI+EC S       N RC N  GSF C C+   E         P  + C    + +++  +    SP  C +  G   C+CP G  Y++K E CIDINEC+ +P  CL   +C N+ G F C CP GF  S +        C D++       C     + +C+    +   + K C          D C+        A + LC  G   V            C  +PG            SF+C CP+G  L+Y    CVD DEC   N C  G TC N  G F C CN+GF
Sbjct: 1969 NDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYEL-------TPDGKNCIDTNECVALPGSC---SPGTCQNLEGSFRCICPPG--YEVKSENCIDINECDEDPNICL-FGSCTNTPGGFQCLCPPGFVLSDNG-----RRCFDTR----QSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCVDTDECSIGNPCGNG-TCTNVIGSFECNCNEGF 2243          

HSP 16 Score: 80.4925 bits (197), Expect = 3.078e-14
Identity = 51/125 (40.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 3015 IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID C+       NG C   + SYRC C+ GY+   +  CID+DEC  S     GD   C NTPGS+ C C  G +    ++ C+DIDEC QN    +   C+N+ G F C CN GF    D K C
Sbjct:  495 IDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDEC-TSNPCTNGD---CVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNC 615          

HSP 17 Score: 80.4925 bits (197), Expect = 3.376e-14
Identity = 105/344 (30.52%), Postives = 139/344 (40.41%), Query Frame = 3
Query: 2409 LTEINLLKDEGHRLT-----FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCID--INECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY--EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +     L +EG+ LT      I  N  V +P S        +    Q+L   F   C       GY+   +NCIDINECD     C   S C NTPG F C C        P F  +     C   R+     +             NG   C  P+ F     + C      E   +PC  +   ++   +F   CP G     S+H    D++EC +S     NG C N  GS+RC C  GY  +Y   + C+D DEC          N  C N  GSF+C C  G E      C DI+EC QN        C+N+FG + CTC  G+    D K C +LD+
Sbjct: 1994 IGSFKCLCNEGYELTPDGKNCIDTNECVALPGSC-------SPGTCQNLEGSFRCICP-----PGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCP-------PGFVLSDNGRRCFDTRQSFCFTNF-----------ENGK--CSVPKAFNTTKAKCCCSKMPGEGWGDPC--ELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVR-CVDTDECSIGNPC---GNGTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPL-LCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDE 2297          

HSP 18 Score: 79.337 bits (194), Expect = 6.617e-14
Identity = 90/308 (29.22%), Postives = 126/308 (40.91%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSG--NGIC----QNMIGSYRCACHNGYEYVEDKGCI-------------------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q+      CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C D++EC          C NS GSF C CP G    +  G V +D    S    G   G+C       +    C C N  +Y   + C                          DI+EC     +C   N +C+N  GS++C C  G E    G  C+DIDEC  N    +   C N+ G ++CTC  G+VF  +T+ CE
Sbjct:  560 CHAGFQRTPTKQACIDIDECIQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKNCVDH-DECTTTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMD-GRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCAN-PDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDGRDINECALDPDICA--NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCE 851          

HSP 19 Score: 77.411 bits (189), Expect = 3.243e-13
Identity = 95/310 (30.65%), Postives = 133/310 (42.90%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTL 3401
            C  GY Q    +CID++EC S  C  N  C+NTPGS+ C C    +    +  C   I+EC        I + V C++   C +++G   C+C  GF  T D K  C+D +EC   N CL+   C N  GSF C C  GF  + +   C D+DEC+   IC  NG C N  GS+RC C  G                   Y  ++   C+       +K  C       G    CQ  P      F  +C  G+     G  DI+EC  +        C N  G + C CN G+  +   + C ++D+ L
Sbjct:  521 CNMGYKQDANGDCIDVDECTSNPC-TNGDCVNTPGSYYCKCHAGFQRTPTKQACI-DIDEC--------IQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKN-CVDHDECTTTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICM-NGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDG-RDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECL 815          

HSP 20 Score: 73.1738 bits (178), Expect = 5.658e-12
Identity = 106/366 (28.96%), Postives = 148/366 (40.44%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGC------------APKIE---ECSFERKI---ISICDAVTCRSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-----------------QASKKLC-------QGDNVVCQN--TPGS--FKCVCP----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            +L  +F   C D   G    + G NC DI+EC D + C N   C+NTPG + C C  + + LNP   GC             P+ +    C+ E  +    S C     ++  N    C   N  E+  +CP G  +      I   DI+EC   P L     C N++GSF C CP+G+  S     C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +                    +K++C       +  N  C+   TPG+  FK +C        +      VDIDEC++         CIN  G F C C  GF +N+    C    E     N+ Q  AD +
Sbjct: 1508 NLPGMFHCICDD---GYELDRTGGNCTDIDECADPINCVN-GLCVNTPGRYECNCPPDFQ-LNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCI 1868          

HSP 21 Score: 72.7886 bits (177), Expect = 7.594e-12
Identity = 71/233 (30.47%), Postives = 95/233 (40.77%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC S    C     C NTPGSF C C            C   ++EC    +    C  +            G   C CP+G+    +   C+D NEC+       A+C N+ GS+ C CP GF +      C D++EC  SK     G C N  G Y C C  GY  V    C+
Sbjct: 2518 PRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCK---CPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE-DVDECDGNHRCQHGCQNIL-----------GGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCNYG-CSNTEGGYLCGCPPGYYRVGQGHCV 2734          

HSP 22 Score: 70.8626 bits (172), Expect = 2.772e-11
Identity = 84/262 (32.06%), Postives = 115/262 (43.89%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASK-KLCQGDNVVCQNTPGSFKCVCPVGL-EYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC  D   C N   C N  GS+ C C++  E       C   I+EC   R +   CD   CR+      + G   C CP G+ +  + E C DINEC  NPC++ A CRN+ GSF+C C  G + S   G + ID        S  G C   I   RC  +     ++ + C  +     S  + C+ D              CP GL   K   C D++EC+     CP G  C+NS G F+C C +G   +   + C
Sbjct:  768 DINECALDPDICAN-GICENLRGSYRCNCNSGYEPDASGRNCI-DIDECLVNRLL---CDNGLCRN------TPGSYSCTCPPGYVFRTETETCEDINECESNPCVNGA-CRNNLGSFNCECSPGSKLS-STGLICID--------SLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTA------------CPRGLARIKGVTCEDVNECEVFPGVCPNGR-CVNSKGSFHCECPEGLTLDGTGRVC 995          

HSP 23 Score: 61.6178 bits (148), Expect = 1.889e-8
Identity = 39/97 (40.21%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            +    C D+DECQA   +CQG N  C NT GSF+C CP G +  E  + C DIDEC       E   C N+ G + C C +G+V + D   C   +T
Sbjct:  269 IRTGACQDVDECQAIPGICQGGN--CINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCIDQRT 363          

HSP 24 Score: 55.4546 bits (132), Expect = 1.201e-6
Identity = 87/330 (26.36%), Postives = 124/330 (37.58%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----DAVTCR-SPANCSSSNGVEFCVCPRGFTYDLKEGCIDIN------ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDK------GCIDIDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  GY+    G NCIDI+EC  + L C +N  C NTPGS+ C C     +      C   I EC     +   C     +  C  SP +  SS G+           D  +G   +N      E N+N    K+ C  + G+           D  CP+G        C D++EC        NG C N  GS+ C C  G                    ++ ED+      G   +D C  +     G        PG+  ++ +CP G  +  +G V        DI+EC+          C N+ G F C CN GF  + + + C
Sbjct:  794 NCNSGYEPDASGRNCIDIDECLVNRLLC-DNGLCRNTPGSYSCTCPPGYVFRTETETCE-DINECESNPCVNGACRNNLGSFNCECSPGSKLSSTGL--------ICIDSLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNC 1113          

HSP 25 Score: 55.0694 bits (131), Expect = 1.739e-6
Identity = 40/100 (40.00%), Postives = 50/100 (50.00%), Query Frame = 3
Query: 2850 CPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CID 3137
            C RGF  +++ G C D++EC   P +     C N+ GSF+C CP G + S     C DIDEC         G C N +GSY C C  GY    D   CID
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGICQGGNCINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCID 360          

HSP 26 Score: 54.6842 bits (130), Expect = 2.314e-6
Identity = 41/104 (39.42%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    IC G G C N +GS+ C C  G++  E  + C DIDEC     +C+     C NT GS+ CVCP G      G   ID+
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGICQG-GNCINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGE--CSNTVGSYFCVCPRGYVTSTDGSRCIDQ 361          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Match: FBN2 (fibrillin 2 [Source:HGNC Symbol;Acc:HGNC:3604])

HSP 1 Score: 146.747 bits (369), Expect = 2.707e-34
Identity = 106/266 (39.85%), Postives = 150/266 (56.39%), Query Frame = 3
Query: 2610 CIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC   D+L C  N+ CIN+PGS+ C C+   + L+P   C  +  EC    +I ++C      S   C    G   C+C  GF     +  C+D++EC  +PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N IGS++C C+ GYE   D K CID +EC A    C      CQN  GSF+C+CP G E K + C+DI+EC ++ N C  GS C N+ GGF C C  GFV +++ + C
Sbjct: 1848 CEDIDECSNGDNL-CQRNADCINSPGSYRCECAAGFK-LSPNGACVDR-NEC---LEIPNVC------SHGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERHPC-GNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCR-NGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPG--TCQNLEGSFRCICPPGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCPPGFVLSDNGRRC 2096          

HSP 2 Score: 122.479 bits (306), Expect = 6.748e-27
Identity = 106/305 (34.75%), Postives = 143/305 (46.89%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSF---ERKIISIC------DAVTCRSPA-------NCSSSNGVEF-CVCPRGFTYDLKEGCI-------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G  +I G  C D+NEC+       N RC+N+ GSF C C            C   ++E+C     E + I         DA  C   A        C      E+  +CPRG  +  +   +       DINEC   P +     CRN+ GSF C C  GF   +    C DIDECR S    G+GIC N  GS+ C C  GYE  ++  K C+DIDEC+ +  LC+G    C NT GSF+C CP+G E     + CVDI+EC  ++       C+N  G + C+CN G+    D + C
Sbjct:  938 DTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCTDIDECRISPDLCGSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGG--TCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGC 1240          

HSP 3 Score: 121.324 bits (303), Expect = 1.380e-26
Identity = 100/281 (35.59%), Postives = 139/281 (49.47%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH---FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC  +    +++C N+ GS+ C CS     +     CA  I+EC        ICD         C++  G   C+C  GF  + D+K  CID+NEC++N   C+    C N+ GSF C C  G  YSV     GC D+DEC   +  C  +  C N+ GS++C+C  G+     K CID+DEC      C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N  G + C C  GF   +D++ C+
Sbjct: 1226 SCNPGYQATPDRQGCTDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCA-DIDEC---ENNPDICDG------GQCTNIPGEYRCLCYDGFMASMDMKT-CIDVNECDLNSNICM-FGECENTKGSFICHCQLG--YSVKKGTTGCTDVDECEIGAHNCDMHASCLNIPGSFKCSCREGWIGNGIK-CIDLDECSNGTHQCS-INAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENINLCENGQCLNVPGAYRCECEMGFTPASDSRSCQ 1490          

HSP 4 Score: 120.168 bits (300), Expect = 3.046e-26
Identity = 96/264 (36.36%), Postives = 129/264 (48.86%), Query Frame = 3
Query: 2616 DINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC +        +C NT GSF C C++       E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  TC N+ GSF C CP G + S     CVDI+EC  S     NG C NMIG+Y+C+C+ GY+   D+ GC DIDEC      C   +  C N+ GS++C C  G      G  C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1073 DINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCT-DIDECRISPDLC---------GSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCTDIDECMIMNGGC---DTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTC 1323          

HSP 5 Score: 113.62 bits (283), Expect = 2.823e-24
Identity = 74/184 (40.22%), Postives = 94/184 (51.09%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D    CID++EC  NPC +   C N+ GS+ C C  GFQ +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC  +     G   +C N  GSFKC+C  G      G  C D+DECQ    C  G  CINS G F C C  G     D + C
Sbjct:  519 CECNMGYKQDANGDCIDVDECTSNPCTN-GDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNCVDHDECTTTNMCLNG---MCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGH-CINSEGSFRCDCPPGLAVGMDGRVC 697          

HSP 6 Score: 113.62 bits (283), Expect = 3.230e-24
Identity = 98/295 (33.22%), Postives = 133/295 (45.08%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G   +  G+ C+D NEC +      N RC+N  GS+ C C+   +  +    C    +   F   + +IC   +       +S   C    G     E C          +CP G  Y       DI+EC + P L     C N+ GSF CFC  G+   +    C+D+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  Q + C     C N+ G F+C C +GF  +     CE
Sbjct: 2326 GMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDG--RDIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPKPC--NYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQ---FLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE 2613          

HSP 7 Score: 111.694 bits (278), Expect = 1.094e-23
Identity = 93/277 (33.57%), Postives = 124/277 (44.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DI+EC  +     N +CINT GSF C+C            C   ++ECS   K            P N  C ++ G   C CPRG+   +  + C D++EC       +  C N+ G F C CP GF    H  C+D +EC     +C   GICQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC   N C   S C N+ G + C C  GF F+  +  C 
Sbjct: 2440 GPGYTTDGR---DIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCI-DLDECSQSPK------------PCNYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG----CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSAS-CYNTLGSYKCACPSGFSFDQFSSACH 2694          

HSP 8 Score: 106.686 bits (265), Expect = 4.048e-22
Identity = 88/259 (33.98%), Postives = 122/259 (47.10%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            G +CID++EC       N  C NT GS+ C C     Y+  E G   K ++EC  ++                C ++ G   C CP GFT      CID NEC   P  C  K  C+N+ GSF C C +GF   +    C D+DEC  +  C     CQN++G YRC C  GY ++ +   C+D +EC          +  C NT GS+KC CP G  + +    C D++EC  +   P    C N+ GG+ C C  G+
Sbjct: 2486 GTSCIDLDECSQSPKPCNYICKNTEGSYQCSCPRG--YVLQEDGKTCKDLDECQTKQHNCQFL----------CVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG--CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNAC---GSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKN-PCNYGCSNTEGGYLCGCPPGY 2725          

HSP 9 Score: 102.064 bits (253), Expect = 8.589e-21
Identity = 101/317 (31.86%), Postives = 139/317 (43.85%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK--------------------------EGCIDINEC-----------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G++    G NC+D +EC +     N  CIN  GSF C C        P F  AP    C+         D   C++P      +C +S G   C CP G    +                            G +  +EC              PC  K +       F   C  G   +V     DI+EC  D  IC+ NGIC+N+ GSYRC C++GYE     + CIDIDEC  ++ LC  DN +C+NTPGS+ C CP G  ++   + C DI+EC ++N C  G+ C N+ G FNC C+ G   ++    C
Sbjct:  602 CNAGFELTTDGKNCVDHDECTTTNMCLNGMCINEDGSFKCICK-------PGFVLAPNGRYCT---------DVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNS-----AEFHGLCSSGVGITVDG--RDINECALDPDICA-NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLC--DNGLCRNTPGSYSCTCPPGYVFRTETETCEDINEC-ESNPCVNGA-CRNNLGSFNCECSPGSKLSSTGLIC 890          

HSP 10 Score: 98.2117 bits (243), Expect = 1.312e-19
Identity = 66/185 (35.68%), Postives = 101/185 (54.59%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GFT+D+  G  +DI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC +   +C  N  C N  GSYRC C  G++   +  C+D +EC     +C   + +C +  GS++C+C  G +  +    C+D+DEC+++  C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1796 GFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCS--HGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERH-PCGNG-TCKNTVGSYNCLCYPGFELTHNNDCLDIDE 1976          

HSP 11 Score: 95.9005 bits (237), Expect = 7.301e-19
Identity = 94/311 (30.23%), Postives = 137/311 (44.05%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDEC--------------------QASKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C +G+   G  CID++EC   +  C  N++C+NTPGS+ C CS  E +    F C+  ++EC+   + I++C+         C +  G   C C  GFT       C DI+EC+        TC N  G F C C  G++     G C DIDEC D   C  NG+C N  G Y C C   ++      GC+D  +  C                      S+  C     +     C+  P      +  +CP G  ++         DIDECQ+     +G  CIN+FG F C C +G+  + DT+ CE
Sbjct: 1394 SCREGWIGNGIKCIDLDECSNGTHQCSINAQCVNTPGSYRCACS--EGFTGDGFTCS-DVDECA---ENINLCEN------GQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYELDRTGGNCTDIDECADPINCV-NGLCVNTPGRYECNCPPDFQLNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICE 1691          

HSP 12 Score: 91.2781 bits (225), Expect = 1.669e-17
Identity = 93/297 (31.31%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP----------------RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF------CP--------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        CINT GSF C C     YL+ +      I+EC     +           P  C ++ G   C+CP                + F Y    G    NE   N  + K  C  +Y     +      CP               GF + +H G  VDIDEC++      NG+C N IGS+RC C  G+ Y +    C DIDEC     LCQ  N  C N+PGS++C C  G +    G CVD +EC +  N C  G  C++  G + C C+ GF  + D   C
Sbjct: 1650 DIDECQELPGLCQGGNCINTFGSFQCECPQGY-YLSEDTRICEDIDECFAHPGVCG---------PGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFN--VTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQ-RNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHG-LCVDLQGSYQCICHNGFKASQDQTMC 1932          

HSP 13 Score: 87.0409 bits (214), Expect = 3.144e-16
Identity = 83/273 (30.40%), Postives = 118/273 (43.22%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNG-ICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            D+NEC +S    +N +CINT GSF C C            C    +ECS      +            C++  G   C C  GF       C DINEC  NP L    C N++GS++C CP G+        C D+DEC +    C   G +C+N+IG++ C C  G     D +GC+D +EC+    +C+  N  C N  GS++C C  G +    G   +D  Q           C   S+  N      C C+ G  + +  + C L  T
Sbjct: 2171 DVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCV-DTDECSIGNPCGN----------GTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICE--NGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGT 2430          

HSP 14 Score: 83.1889 bits (204), Expect = 4.411e-15
Identity = 97/323 (30.03%), Postives = 135/323 (41.80%), Query Frame = 3
Query: 2568 DVNCGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGYEYVED-------------KGCI----------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPK 3419
            + NC +G++     NC DINEC         RC+NT GS+ C C         +  C   ++EC+   + +  C++        C +  G   C+CP G       EGC+D NEC   P + +   C N  GS+ C C +GFQ S         EC D++           IC      +N++    C C  G  +                K C           DIDEC+    LC   N  C NT GSF+C C VG      G  C+D+DEC Q+   P    C N+ G + C+C +G+V   D K C   K L+  Q K
Sbjct: 2236 ECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCK-DLDECA---EGLHDCESRG----MMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSG-----TECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDGRDIDECKVMPNLCT--NGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPK-PCNYICKNTEGSYQCSCPRGYVLQEDGKTC---KDLDECQTK 2539          

HSP 15 Score: 82.8037 bits (203), Expect = 7.184e-15
Identity = 92/298 (30.87%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2604 DNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDI-------DECQ--------ASKKLCQ-GDNVV------------CQNTPG------------SFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            ++C+DI+EC S       N RC N  GSF C C+   E         P  + C    + +++  +    SP  C +  G   C+CP G  Y++K E CIDINEC+ +P  CL   +C N+ G F C CP GF  S +        C D++       C     + +C+    +   + K C          D C+        A + LC  G   V            C  +PG            SF+C CP+G  L+Y    CVD DEC   N C  G TC N  G F C CN+GF
Sbjct: 1969 NDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYEL-------TPDGKNCIDTNECVALPGSC---SPGTCQNLEGSFRCICPPG--YEVKSENCIDINECDEDPNICL-FGSCTNTPGGFQCLCPPGFVLSDNG-----RRCFDTR----QSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCVDTDECSIGNPCGNG-TCTNVIGSFECNCNEGF 2243          

HSP 16 Score: 80.4925 bits (197), Expect = 3.078e-14
Identity = 51/125 (40.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 3015 IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID C+       NG C   + SYRC C+ GY+   +  CID+DEC  S     GD   C NTPGS+ C C  G +    ++ C+DIDEC QN    +   C+N+ G F C CN GF    D K C
Sbjct:  495 IDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDEC-TSNPCTNGD---CVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNC 615          

HSP 17 Score: 80.4925 bits (197), Expect = 3.376e-14
Identity = 105/344 (30.52%), Postives = 139/344 (40.41%), Query Frame = 3
Query: 2409 LTEINLLKDEGHRLT-----FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCID--INECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY--EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +     L +EG+ LT      I  N  V +P S        +    Q+L   F   C       GY+   +NCIDINECD     C   S C NTPG F C C        P F  +     C   R+     +             NG   C  P+ F     + C      E   +PC  +   ++   +F   CP G     S+H    D++EC +S     NG C N  GS+RC C  GY  +Y   + C+D DEC          N  C N  GSF+C C  G E      C DI+EC QN        C+N+FG + CTC  G+    D K C +LD+
Sbjct: 1994 IGSFKCLCNEGYELTPDGKNCIDTNECVALPGSC-------SPGTCQNLEGSFRCICP-----PGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCP-------PGFVLSDNGRRCFDTRQSFCFTNF-----------ENGK--CSVPKAFNTTKAKCCCSKMPGEGWGDPC--ELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVR-CVDTDECSIGNPC---GNGTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPL-LCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDE 2297          

HSP 18 Score: 79.337 bits (194), Expect = 6.617e-14
Identity = 90/308 (29.22%), Postives = 126/308 (40.91%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSG--NGIC----QNMIGSYRCACHNGYEYVEDKGCI-------------------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q+      CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C D++EC          C NS GSF C CP G    +  G V +D    S    G   G+C       +    C C N  +Y   + C                          DI+EC     +C   N +C+N  GS++C C  G E    G  C+DIDEC  N    +   C N+ G ++CTC  G+VF  +T+ CE
Sbjct:  560 CHAGFQRTPTKQACIDIDECIQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKNCVDH-DECTTTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMD-GRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCAN-PDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDGRDINECALDPDICA--NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCE 851          

HSP 19 Score: 77.411 bits (189), Expect = 3.243e-13
Identity = 95/310 (30.65%), Postives = 133/310 (42.90%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTL 3401
            C  GY Q    +CID++EC S  C  N  C+NTPGS+ C C    +    +  C   I+EC        I + V C++   C +++G   C+C  GF  T D K  C+D +EC   N CL+   C N  GSF C C  GF  + +   C D+DEC+   IC  NG C N  GS+RC C  G                   Y  ++   C+       +K  C       G    CQ  P      F  +C  G+     G  DI+EC  +        C N  G + C CN G+  +   + C ++D+ L
Sbjct:  521 CNMGYKQDANGDCIDVDECTSNPC-TNGDCVNTPGSYYCKCHAGFQRTPTKQACI-DIDEC--------IQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKN-CVDHDECTTTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICM-NGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDG-RDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECL 815          

HSP 20 Score: 73.1738 bits (178), Expect = 5.658e-12
Identity = 106/366 (28.96%), Postives = 148/366 (40.44%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGC------------APKIE---ECSFERKI---ISICDAVTCRSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-----------------QASKKLC-------QGDNVVCQN--TPGS--FKCVCP----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            +L  +F   C D   G    + G NC DI+EC D + C N   C+NTPG + C C  + + LNP   GC             P+ +    C+ E  +    S C     ++  N    C   N  E+  +CP G  +      I   DI+EC   P L     C N++GSF C CP+G+  S     C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +                    +K++C       +  N  C+   TPG+  FK +C        +      VDIDEC++         CIN  G F C C  GF +N+    C    E     N+ Q  AD +
Sbjct: 1508 NLPGMFHCICDD---GYELDRTGGNCTDIDECADPINCVN-GLCVNTPGRYECNCPPDFQ-LNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCI 1868          

HSP 21 Score: 72.7886 bits (177), Expect = 7.594e-12
Identity = 71/233 (30.47%), Postives = 95/233 (40.77%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC S    C     C NTPGSF C C            C   ++EC    +    C  +            G   C CP+G+    +   C+D NEC+       A+C N+ GS+ C CP GF +      C D++EC  SK     G C N  G Y C C  GY  V    C+
Sbjct: 2518 PRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCK---CPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE-DVDECDGNHRCQHGCQNIL-----------GGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCNYG-CSNTEGGYLCGCPPGYYRVGQGHCV 2734          

HSP 22 Score: 70.8626 bits (172), Expect = 2.772e-11
Identity = 84/262 (32.06%), Postives = 115/262 (43.89%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASK-KLCQGDNVVCQNTPGSFKCVCPVGL-EYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC  D   C N   C N  GS+ C C++  E       C   I+EC   R +   CD   CR+      + G   C CP G+ +  + E C DINEC  NPC++ A CRN+ GSF+C C  G + S   G + ID        S  G C   I   RC  +     ++ + C  +     S  + C+ D              CP GL   K   C D++EC+     CP G  C+NS G F+C C +G   +   + C
Sbjct:  768 DINECALDPDICAN-GICENLRGSYRCNCNSGYEPDASGRNCI-DIDECLVNRLL---CDNGLCRN------TPGSYSCTCPPGYVFRTETETCEDINECESNPCVNGA-CRNNLGSFNCECSPGSKLS-STGLICID--------SLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTA------------CPRGLARIKGVTCEDVNECEVFPGVCPNGR-CVNSKGSFHCECPEGLTLDGTGRVC 995          

HSP 23 Score: 61.6178 bits (148), Expect = 1.889e-8
Identity = 39/97 (40.21%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            +    C D+DECQA   +CQG N  C NT GSF+C CP G +  E  + C DIDEC       E   C N+ G + C C +G+V + D   C   +T
Sbjct:  269 IRTGACQDVDECQAIPGICQGGN--CINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCIDQRT 363          

HSP 24 Score: 55.4546 bits (132), Expect = 1.201e-6
Identity = 87/330 (26.36%), Postives = 124/330 (37.58%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----DAVTCR-SPANCSSSNGVEFCVCPRGFTYDLKEGCIDIN------ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDK------GCIDIDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  GY+    G NCIDI+EC  + L C +N  C NTPGS+ C C     +      C   I EC     +   C     +  C  SP +  SS G+           D  +G   +N      E N+N    K+ C  + G+           D  CP+G        C D++EC        NG C N  GS+ C C  G                    ++ ED+      G   +D C  +     G        PG+  ++ +CP G  +  +G V        DI+EC+          C N+ G F C CN GF  + + + C
Sbjct:  794 NCNSGYEPDASGRNCIDIDECLVNRLLC-DNGLCRNTPGSYSCTCPPGYVFRTETETCE-DINECESNPCVNGACRNNLGSFNCECSPGSKLSSTGL--------ICIDSLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNC 1113          

HSP 25 Score: 55.0694 bits (131), Expect = 1.739e-6
Identity = 40/100 (40.00%), Postives = 50/100 (50.00%), Query Frame = 3
Query: 2850 CPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CID 3137
            C RGF  +++ G C D++EC   P +     C N+ GSF+C CP G + S     C DIDEC         G C N +GSY C C  GY    D   CID
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGICQGGNCINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCID 360          

HSP 26 Score: 54.6842 bits (130), Expect = 2.314e-6
Identity = 41/104 (39.42%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    IC G G C N +GS+ C C  G++  E  + C DIDEC     +C+     C NT GS+ CVCP G      G   ID+
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGICQG-GNCINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGE--CSNTVGSYFCVCPRGYVTSTDGSRCIDQ 361          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Match: FBN3 (fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794])

HSP 1 Score: 144.436 bits (363), Expect = 1.350e-33
Identity = 104/275 (37.82%), Postives = 145/275 (52.73%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C D++EC S    C  N+ CIN PGS+ C C+   + L+P   C  +  EC   R+I ++C      S  +C  + G   C+C RGF     +  C+DI+EC+  PC    TC+N  GS++C C  GF  + +  CVD DEC     ++C   G C N  GS+ C C +G+E   D K C+D +EC +    C      CQN  GSF+C+CP G + +   C+DIDEC ++ N C  G TC NS G F C C  GFV +++   C
Sbjct: 1751 GFNYNSILLACEDVDECGSRESPCQQNADCINIPGSYRCKCTRGYK-LSPGGACVGR-NEC---REIPNVC------SHGDCMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQPC-GNGTCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCR-FGHCLNTAGSFHCLCQDGFELTADGKNCVDTNECLSLAGTCL--PGTCQNLEGSFRCICPPGFQVQSDHCIDIDECSEEPNLCLFG-TCTNSPGSFQCLCPPGFVLSDNGHRC 2009          

HSP 2 Score: 135.961 bits (341), Expect = 4.921e-31
Identity = 115/309 (37.22%), Postives = 144/309 (46.60%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNN-----------EEYLNPEFGCAPKIEECSFE-----RKIISICD-----AVTCRSPANCSSSNGVEFC-VCPRGFTY---DLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ ++ G  C D+NEC+S      N RC+NT GSF C C              +  L P F      +EC        R  +  C       V C +   C     +EF  +CPRG  +   D   G     D+NEC + P L    TCRN+ GSF C C  GF        C DIDECR S    G G C N  GS+ C C  GYE  ++  K C+D+DEC     LC+G    C NT GS+KC CP G E   KG  C DIDEC   +  CP G  C+N  G F C+C+ GF    D + C
Sbjct:  852 DPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCF-LRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEA---CPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALDAQERNCTDIDECRISPDLCGQGTCVNTPGSFECECFPGYESGFMLMKNCMDVDECARDPLLCRGG--TCTNTDGSYKCQCPPGHELTAKGTACEDIDECSLSDGLCPHGQ-CVNVIGAFQCSCHAGFQSTPDRQGC 1153          

HSP 3 Score: 118.242 bits (295), Expect = 1.050e-25
Identity = 98/314 (31.21%), Postives = 140/314 (44.59%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   +     DVD          R    L  H   +  +     +C  GY        C+D++EC  +       C NT GSF+C C     YL  E G   K ++EC+  +                C ++ G   C CP GFT    + C D +EC+    PC     C N+ GSF C C +GF   S   GC D++EC     C     CQN +G YRC+C  G+ ++ +   C+D +EC  S   C   +  C+NT G F+CVCP G ++ +   GC ++DEC      P   +C N+ GGF C C +G+
Sbjct: 2353 HGSGYTAEGRDVD--------ECRMLAHLCAHGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKPCTFLCKNTKGSFLCSCPRG--YLLEEDGRTCKDLDECTSRQHNCQF----------LCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG--CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTC--GSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRG-PCSYSCANTPGGFLCGCPQGY 2640          

HSP 4 Score: 115.161 bits (287), Expect = 8.989e-25
Identity = 107/328 (32.62%), Postives = 150/328 (45.73%), Query Frame = 3
Query: 2439 GHRLTFI-TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLC--QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            GH LT   T   D+D            +  L  H   V  I     +C  G+Q   D   C+DINEC       +  CINT GS+ C C      +     CA  ++EC    ++   CD        +C++  G   C+C  GF  T D++  C+D++EC++NP  CL    C N+ GSF C C  G+       GC D+DEC      C  +  C N+ GS+ C C  G+  V D   C D+DEC + +  C  +GD   C N PGS++C C  G       C D DEC +N    +   C+N+ GG+ C C  GF    D + C+
Sbjct: 1101 GHELTAKGTACEDID--------ECSLSDGLCPHGQCVNVIGAFQCSCHAGFQSTPDRQGCVDINECRVQNGGCDVHCINTEGSYRCSCGQGYSLMPDGRACA-DVDECEENPRV---CDQ------GHCTNMPGGHRCLCYDGFMATPDMRT-CVDVDECDLNPHICL-HGDCENTKGSFVCHCQLGYMVRKGATGCSDVDECEVGGHNCDSHASCLNIPGSFSCRCLPGW--VGDGFECHDLDECVSQEHRCSPRGD---CLNVPGSYRCTCRQGFAGDGFFCEDRDECAENVDLCDNGQCLNAPGGYRCECEMGFDPTEDHRACQ 1403          

HSP 5 Score: 113.235 bits (282), Expect = 3.486e-24
Identity = 95/288 (32.99%), Postives = 131/288 (45.49%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G GY   G    D++EC  LA H   +  CIN+ GSF C+C            C   ++ECS   K  +            C ++ G   C CPRG+  + ++G  C D++EC       +  C N+ G+F C CP GF    H  C D DEC      C  +G C N  GS+RC CH G+  V    GC D++EC    +   G    CQN  G ++C CP G     +   CVD +EC  +      ++C N+ GGF C C  GF F+     C E+D+      P
Sbjct: 2354 GSGYTAEGR---DVDECRMLA-HLCAHGECINSLGSFRCHCQAGYTPDATATTCL-DMDECSQVPKPCTF----------LCKNTKGSFLCSCPRGYLLE-EDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG----CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGP 2620          

HSP 6 Score: 106.301 bits (264), Expect = 4.228e-22
Identity = 70/187 (37.43%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG---LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C +++G   C CP G++ D     C+D +EC++     + TC N  G F+C C  GF+  +   C DIDEC  +  +C+    C N  GSY C C  GY   ED   C D+DEC   ++ C    + C+N  G+F CVCP G   L    +GC D +EC  Q + C  G  C+N+ G F C C++GF
Sbjct: 2100 CVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFR--CHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGR-CVNTAGSFRCDCDEGF 2283          

HSP 7 Score: 103.605 bits (257), Expect = 3.161e-21
Identity = 73/184 (39.67%), Postives = 95/184 (51.63%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+T D++  CID++EC  +PC     C N  G++ C C  GFQ +     CVD+DEC  S      G C N  GS++C C+ G+E   D K C+D +EC A+  +C   N VC N  GSF C+C  G      G  C+DIDECQ    C  G  C N+ G F C C  G     D + C
Sbjct:  433 CECNVGYTQDVRGECIDVDECTSSPC-HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCVNTEGSFQCVCNAGFELSPDGKNCVDHNEC-ATSTMCV--NGVCLNEDGSFSCLCKPGFLLAPGGHYCMDIDECQTPGICVNGH-CTNTEGSFRCQCLGGLAVGTDGRVC 611          

HSP 8 Score: 100.908 bits (250), Expect = 1.914e-20
Identity = 76/232 (32.76%), Postives = 115/232 (49.57%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG 3254
            +C +GY  ++ G  C D++EC S   +    C+NT G+F C C        P F    +  +  F+    S      C +  +C ++ G   C C +GFT      GC D+NEC+  P   +  C+N  G + C CP+GF Q+S    CVD +EC  S    G+  C+N +G +RC C +G+++ +   GC ++DEC   +  C   +  C NTPG F C CP G     +G
Sbjct: 2430 SCPRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCP-------PGF---TQHHQACFDNDECSA-QPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECD-GPHRCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPC---SYSCANTPGGFLCGCPQGYFRAGQG 2646          

HSP 9 Score: 97.0561 bits (240), Expect = 2.974e-19
Identity = 81/236 (34.32%), Postives = 116/236 (49.15%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESM----KNYMDIVKDMLKNIITDYKLKSLA 3530
            GF  D+  G  +DI+EC   P +     C N  GSF C CP GF Y S+   C D+DEC  R+S  C  N  C N+ GSYRC C  GY+      C+  +EC+    +C  GD   C +T GS+ C+C  G +    +  C+DIDEC +   C  G TC N  G +NC C  GFV  ++    + D+   +V         ++T+ S     ++  ++  D  KN +   +  SLA
Sbjct: 1709 GFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESP-CQQNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD---CMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQ-PCGNG-TCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFELTADG-KNCVDTNECLSLA 1937          

HSP 10 Score: 91.2781 bits (225), Expect = 1.578e-17
Identity = 97/289 (33.56%), Postives = 129/289 (44.64%), Query Frame = 3
Query: 2607 NCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECRDSKI---------CS---GNG------IC-QNMIGSYRCACHNGYEYVE--DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C+D +EC +L         C+NT GSF C C +  E            + C    + +S+  A TC  P  C +  G   C+CP GF     + CIDI+EC+  P  CL   TC NS GSF C CP GF            + S  F   +  +C   K          CS   G G      +C Q    +++  C  G+  V   D    D++EC  +  +C   N VC NT GSF+C CP G  L++    CVD DEC   + C +G TC N  GGF C C  GF
Sbjct: 1883 DCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFEL-------TADGKNCVDTNECLSL--AGTCL-PGTCQNLEGSFRCICPPGFQVQ-SDHCIDIDECSEEPNLCL-FGTCTNSPGSFQCLCPPGFVLSDNGHRCFDTRQSFCFTRFEAGKCSVPKAFNTTKTRCCCSKRPGEGWGDPCELCPQEGSAAFQELCPFGHGAVPGPDDSREDVNECAENPGVCT--NGVCVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQG-TCTNVIGGFECACADGF 2156          

HSP 11 Score: 89.3521 bits (220), Expect = 6.764e-17
Identity = 92/307 (29.97%), Postives = 127/307 (41.37%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDL-KEGCIDIN-ECNMNPCLDKATCRNSY------------------------------GSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            G NC+D NEC +     N  C+N  GSF C C        P F  AP    C          D   C++P      +C+++ G   C C  G         C+D +        ++K +C   +                                F   C  G   +      DI+EC  D ++C+ NG+C+N+ GSYRC C+ GYE     K C D+DEC  +  LC  DN  CQN+PGS+ C CP G  + +    C D+DEC  ++ C  G  C N  G + C C  G   +    FC LD T
Sbjct:  526 GKNCVDHNECATSTMCVNGVCLNEDGSFSCLC-------KPGFLLAPGGHYCM---------DIDECQTPGICVNGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG--RDINECALDPEVCA-NGVCENLRGSYRCVCNLGYEAGASGKDCTDVDECALNSLLC--DNGWCQNSPGSYSCSCPPGFHFWQDTEICKDVDEC-LSSPCVSG-VCRNLAGSYTCKCGPGSRLDPSGTFC-LDST 808          

HSP 12 Score: 88.9669 bits (219), Expect = 8.208e-17
Identity = 62/168 (36.90%), Postives = 84/168 (50.00%), Query Frame = 3
Query: 2889 IDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            IDI     N CL+   C  +  S+ C C  G+   V   C+D+DEC  S     +G C N+ G+Y C C+ G++    +  C+D+DEC  S  LC     V  NT GSF+CVC  G E    G  CVD +EC  +  C  G  C+N  G F+C C  GF+      +C
Sbjct:  409 IDICRHFTNLCLN-GRCLPTPSSYRCECNVGYTQDVRGECIDVDECTSSPC--HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCV--NTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNG-VCLNEDGSFSCLCKPGFLLAPGGHYC 570          

HSP 13 Score: 87.4261 bits (215), Expect = 2.369e-16
Identity = 73/214 (34.11%), Postives = 98/214 (45.79%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKI-CSGNGI-CQNMIGSYRCACHNGYEYV--EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            D+NEC ++     N  C+NT GSF C C            C    +ECS                   C++  G   C C  GF   L   C DI+EC++NP L    C N+ GS+ C CP G+        C D+DEC D +  C   G+ C+N+IG++ C C  G   +    +GC D +EC A   LC   N  C NT GSF+C C  G +
Sbjct: 2084 DVNECAENPGVCTNGVCVNTDGSFRCECPFGYSLDFTGINCV-DTDECSVGHPC----------GQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCV--NGRCVNTAGSFRCDCDEGFQ 2284          

HSP 14 Score: 85.1149 bits (209), Expect = 1.340e-15
Identity = 96/341 (28.15%), Postives = 131/341 (38.42%), Query Frame = 3
Query: 2460 TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            T+N  +DI R    L +       + L       C+   C  GY Q +   CID++EC S  CH+   C+N PG++ C C    +       C   ++EC     +  +           C ++ G   CVC  GF      + C+D NEC  +       C N  GSF C C  GF  +   H+ C+DIDEC+   IC  NG C N  GS+RC C  G                   Y  +E   C        +K  C       G    CQ  P      F+ +C  GL     G  DI+EC  +        C N  G + C CN G+      K C
Sbjct:  404 TLNQTIDICRHFTNLCLN-----GRCLPTPSSYRCE---CNVGYTQDVRGECIDVDECTSSPCHHGD-CVNIPGTYHCRCYPGFQATPTRQACV-DVDECIVSGGLCHL---------GRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNGVCLNEDGSFSCLCKPGFLLAPGGHY-CMDIDECQTPGICV-NGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG-RDINECALDPEVCANGVCENLRGSYRCVCNLGYEAGASGKDC 722          

HSP 15 Score: 80.8777 bits (198), Expect = 2.503e-14
Identity = 91/298 (30.54%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2616 DINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CP---------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L   C     C+NT GSF C C     +L+        I+ECS    I           P  C ++ G   CVCP  +   +    C+D+              NE   N  + +  C  SY     +      CP                GF   +H G  +DIDEC +      NGIC N IGS+RC C  G+ Y      C D+DEC + +  CQ  N  C N PGS++C C  G +    G CV  +EC++  N C  G  C+++ G + C C++GF  + D   C
Sbjct: 1563 DIDECQELPGLCQGGD-CVNTFGSFQCECPPGY-HLSEHTRICEDIDECSTHSGICG---------PGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFN--VTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESPCQ-QNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD-CMDTEGSYMCLCHRGFQASADQTLC 1845          

HSP 16 Score: 80.8777 bits (198), Expect = 2.655e-14
Identity = 71/235 (30.21%), Postives = 102/235 (43.40%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK-----------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNP-CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + +   RT K+L +  S+           V   TC+   C  G+ +    C D +EC +    C  +  C NTPGSF C C      ++   GC           + ++ CD    R    C +  G   C CP+GFT +     C+D NEC ++P     A+CRN+ G F C CP GF +    G C ++DEC   +  CS +  C N  G + C C  GY       C+
Sbjct: 2432 PRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCR---CPPGFTQHHQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGC-----------EDVNECDGPH-RCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPCSYS--CANTPGGFLCGCPQGYFRAGQGHCV 2649          

HSP 17 Score: 80.1073 bits (196), Expect = 3.812e-14
Identity = 106/337 (31.45%), Postives = 143/337 (42.43%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERK-------------IISICDAVTC----RSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG---CIDIDE----------CQ------ASKKLC-------QGDNVVCQNTPGS----FKCVC----PVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+  + G NC DINEC D + C N   CINTPGS++C C  + E LNP   GC   +   C  E                + +  A  C    R+  N    C  +N  E+  +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  S H   C DIDEC       G G C N +G+Y C C    EY++  G   C+D+ +          CQ       ++K+C       Q  N  C+  P      ++ +C    P  L     G  +DIDEC +         CIN  G F C C  GF +N+    CE
Sbjct: 1430 CNGGYELDRGGGNCTDINECADPVNCINGV-CINTPGSYLCSCPQDFE-LNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICEDIDECSTHSGICGPGTCYNTLGNYTCVCP--AEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACE 1762          

HSP 18 Score: 77.7962 bits (190), Expect = 1.999e-13
Identity = 84/310 (27.10%), Postives = 126/310 (40.65%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID---------------------------IDECQASKKLCQGDNVVCQNTPGS----FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G  C D++EC S    C     C+N PGS+ C C   + +    F C  + +EC+   + + +CD         C ++ G   C C  GF   +    C D++EC         +C N  G F C C  G++     G C DI+EC D   C  NG+C N  GSY C+C   +E      GC+D                           +        L +     C+  P +    ++ +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  +  T+ CE
Sbjct: 1308 CLPGWVGDGFECHDLDECVSQEHRCSPRGDCLNVPGSYRCTC--RQGFAGDGFFCEDR-DECA---ENVDLCDN------GQCLNAPGGYRCECEMGFDPTEDHRACQDVDECAQGNLCAFGSCENLPGMFRCICNGGYELDRGGGNCTDINECADPVNCI-NGVCINTPGSYLCSCPQDFELNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICE 1604          

HSP 19 Score: 72.0182 bits (175), Expect = 1.263e-11
Identity = 86/317 (27.13%), Postives = 128/317 (40.38%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEE---YLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINEC--NMNPCLDKAT-CRNSYGSFDCFCPKGFQ--YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQN-----------------------------TPG--SFKCVCPVGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G NC+D +EC          C N  G F C C++  E    +  E      I+ECS    + +            C ++ G   C CP G+T  L+E    C D++EC      C  +   C+N  G+F C CP G +       GC D +EC        NG C N  GS+RC C  G++       C DI +     ++ Q    +C++                              PG  +++ +CP G  Y  +G  D+DEC+   + C  G  CINS G F C C  G+  +     C ++D+   V +P
Sbjct: 2121 GINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCE-----DIDECSLNPLLCAF----------RCHNTEGSYLCTCPAGYT--LREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQ---TMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPGTSAYRKLCPHGSGYTAEG-RDVDECRMLAHLCAHGE-CINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKP 2415          

HSP 20 Score: 65.4698 bits (158), Expect = 1.145e-9
Identity = 89/305 (29.18%), Postives = 126/305 (41.31%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY--DLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDE-----------CRD------------------------SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            C DI+EC         RC NT GS++C C      L  +      ++EC+  ++    C A        C +  G   CVCP G        EGC D NEC+  P  C++   C N+ GSF C C +GFQ S     C DI +           CR                          ++C   G       +YR  C +G  Y  +    D+DEC+    LC   +  C N+ GSF+C C  G   +     C+D+DEC Q    P    C N+ G F C+C +G++   D + C +LD+
Sbjct: 2164 CEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYT-LREDGAMCRDVDECADGQQD---CHARG----MECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVN-GRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPG-----TSAYRKLCPHGSGYTAEG--RDVDECRMLAHLCA--HGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPK-PCTFLCKNTKGSFLCSCPRGYLLEEDGRTCKDLDE 2449          

HSP 21 Score: 53.9138 bits (128), Expect = 3.426e-6
Identity = 85/326 (26.07%), Postives = 128/326 (39.26%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRN--------SYGS------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLCQGD-----NVVCQNTPGS----FKCVCPVGLEYKEKGCV-------DIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G +C D++EC  +SL C +N  C N+PGS+ C C     +      C   ++EC     +  +C     + TC+            FC+     T  LK   I  + C +N  L  A+ R+        ++GS       D  C +GF       C D++EC        NG C N  GS+RC C  G       + C+D+           DEC  +        +C         V C+  P      F  +CP GL +  +  +       D++EC+     C  G TC N+ G F+C C  GF  +   + C
Sbjct:  709 CNLGYEAGASGKDCTDVDECALNSLLC-DNGWCQNSPGSYSCSCPPGFHFWQDTEICK-DVDECLSSPCVSGVCRNLAGSYTCKCGPGSRLDPSGTFCLDSTKGTCWLK---IQESRCEVN--LQGASLRSECCATLGAAWGSPCERCEIDPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCFLRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEACPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHG-TCRNTVGSFHCACAGGFALDAQERNC 1026          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Match: FBN3 (fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794])

HSP 1 Score: 144.436 bits (363), Expect = 1.350e-33
Identity = 104/275 (37.82%), Postives = 145/275 (52.73%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C D++EC S    C  N+ CIN PGS+ C C+   + L+P   C  +  EC   R+I ++C      S  +C  + G   C+C RGF     +  C+DI+EC+  PC    TC+N  GS++C C  GF  + +  CVD DEC     ++C   G C N  GS+ C C +G+E   D K C+D +EC +    C      CQN  GSF+C+CP G + +   C+DIDEC ++ N C  G TC NS G F C C  GFV +++   C
Sbjct: 1751 GFNYNSILLACEDVDECGSRESPCQQNADCINIPGSYRCKCTRGYK-LSPGGACVGR-NEC---REIPNVC------SHGDCMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQPC-GNGTCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCR-FGHCLNTAGSFHCLCQDGFELTADGKNCVDTNECLSLAGTCL--PGTCQNLEGSFRCICPPGFQVQSDHCIDIDECSEEPNLCLFG-TCTNSPGSFQCLCPPGFVLSDNGHRC 2009          

HSP 2 Score: 135.961 bits (341), Expect = 4.921e-31
Identity = 115/309 (37.22%), Postives = 144/309 (46.60%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNN-----------EEYLNPEFGCAPKIEECSFE-----RKIISICD-----AVTCRSPANCSSSNGVEFC-VCPRGFTY---DLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ ++ G  C D+NEC+S      N RC+NT GSF C C              +  L P F      +EC        R  +  C       V C +   C     +EF  +CPRG  +   D   G     D+NEC + P L    TCRN+ GSF C C  GF        C DIDECR S    G G C N  GS+ C C  GYE  ++  K C+D+DEC     LC+G    C NT GS+KC CP G E   KG  C DIDEC   +  CP G  C+N  G F C+C+ GF    D + C
Sbjct:  852 DPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCF-LRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEA---CPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALDAQERNCTDIDECRISPDLCGQGTCVNTPGSFECECFPGYESGFMLMKNCMDVDECARDPLLCRGG--TCTNTDGSYKCQCPPGHELTAKGTACEDIDECSLSDGLCPHGQ-CVNVIGAFQCSCHAGFQSTPDRQGC 1153          

HSP 3 Score: 118.242 bits (295), Expect = 1.050e-25
Identity = 98/314 (31.21%), Postives = 140/314 (44.59%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   +     DVD          R    L  H   +  +     +C  GY        C+D++EC  +       C NT GSF+C C     YL  E G   K ++EC+  +                C ++ G   C CP GFT    + C D +EC+    PC     C N+ GSF C C +GF   S   GC D++EC     C     CQN +G YRC+C  G+ ++ +   C+D +EC  S   C   +  C+NT G F+CVCP G ++ +   GC ++DEC      P   +C N+ GGF C C +G+
Sbjct: 2353 HGSGYTAEGRDVD--------ECRMLAHLCAHGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKPCTFLCKNTKGSFLCSCPRG--YLLEEDGRTCKDLDECTSRQHNCQF----------LCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG--CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTC--GSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRG-PCSYSCANTPGGFLCGCPQGY 2640          

HSP 4 Score: 115.161 bits (287), Expect = 8.989e-25
Identity = 107/328 (32.62%), Postives = 150/328 (45.73%), Query Frame = 3
Query: 2439 GHRLTFI-TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLC--QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            GH LT   T   D+D            +  L  H   V  I     +C  G+Q   D   C+DINEC       +  CINT GS+ C C      +     CA  ++EC    ++   CD        +C++  G   C+C  GF  T D++  C+D++EC++NP  CL    C N+ GSF C C  G+       GC D+DEC      C  +  C N+ GS+ C C  G+  V D   C D+DEC + +  C  +GD   C N PGS++C C  G       C D DEC +N    +   C+N+ GG+ C C  GF    D + C+
Sbjct: 1101 GHELTAKGTACEDID--------ECSLSDGLCPHGQCVNVIGAFQCSCHAGFQSTPDRQGCVDINECRVQNGGCDVHCINTEGSYRCSCGQGYSLMPDGRACA-DVDECEENPRV---CDQ------GHCTNMPGGHRCLCYDGFMATPDMRT-CVDVDECDLNPHICL-HGDCENTKGSFVCHCQLGYMVRKGATGCSDVDECEVGGHNCDSHASCLNIPGSFSCRCLPGW--VGDGFECHDLDECVSQEHRCSPRGD---CLNVPGSYRCTCRQGFAGDGFFCEDRDECAENVDLCDNGQCLNAPGGYRCECEMGFDPTEDHRACQ 1403          

HSP 5 Score: 113.235 bits (282), Expect = 3.486e-24
Identity = 95/288 (32.99%), Postives = 131/288 (45.49%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G GY   G    D++EC  LA H   +  CIN+ GSF C+C            C   ++ECS   K  +            C ++ G   C CPRG+  + ++G  C D++EC       +  C N+ G+F C CP GF    H  C D DEC      C  +G C N  GS+RC CH G+  V    GC D++EC    +   G    CQN  G ++C CP G     +   CVD +EC  +      ++C N+ GGF C C  GF F+     C E+D+      P
Sbjct: 2354 GSGYTAEGR---DVDECRMLA-HLCAHGECINSLGSFRCHCQAGYTPDATATTCL-DMDECSQVPKPCTF----------LCKNTKGSFLCSCPRGYLLE-EDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG----CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGP 2620          

HSP 6 Score: 106.301 bits (264), Expect = 4.228e-22
Identity = 70/187 (37.43%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG---LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C +++G   C CP G++ D     C+D +EC++     + TC N  G F+C C  GF+  +   C DIDEC  +  +C+    C N  GSY C C  GY   ED   C D+DEC   ++ C    + C+N  G+F CVCP G   L    +GC D +EC  Q + C  G  C+N+ G F C C++GF
Sbjct: 2100 CVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFR--CHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGR-CVNTAGSFRCDCDEGF 2283          

HSP 7 Score: 103.605 bits (257), Expect = 3.161e-21
Identity = 73/184 (39.67%), Postives = 95/184 (51.63%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+T D++  CID++EC  +PC     C N  G++ C C  GFQ +     CVD+DEC  S      G C N  GS++C C+ G+E   D K C+D +EC A+  +C   N VC N  GSF C+C  G      G  C+DIDECQ    C  G  C N+ G F C C  G     D + C
Sbjct:  433 CECNVGYTQDVRGECIDVDECTSSPC-HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCVNTEGSFQCVCNAGFELSPDGKNCVDHNEC-ATSTMCV--NGVCLNEDGSFSCLCKPGFLLAPGGHYCMDIDECQTPGICVNGH-CTNTEGSFRCQCLGGLAVGTDGRVC 611          

HSP 8 Score: 100.908 bits (250), Expect = 1.914e-20
Identity = 76/232 (32.76%), Postives = 115/232 (49.57%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG 3254
            +C +GY  ++ G  C D++EC S   +    C+NT G+F C C        P F    +  +  F+    S      C +  +C ++ G   C C +GFT      GC D+NEC+  P   +  C+N  G + C CP+GF Q+S    CVD +EC  S    G+  C+N +G +RC C +G+++ +   GC ++DEC   +  C   +  C NTPG F C CP G     +G
Sbjct: 2430 SCPRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCP-------PGF---TQHHQACFDNDECSA-QPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECD-GPHRCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPC---SYSCANTPGGFLCGCPQGYFRAGQG 2646          

HSP 9 Score: 97.0561 bits (240), Expect = 2.974e-19
Identity = 81/236 (34.32%), Postives = 116/236 (49.15%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESM----KNYMDIVKDMLKNIITDYKLKSLA 3530
            GF  D+  G  +DI+EC   P +     C N  GSF C CP GF Y S+   C D+DEC  R+S  C  N  C N+ GSYRC C  GY+      C+  +EC+    +C  GD   C +T GS+ C+C  G +    +  C+DIDEC +   C  G TC N  G +NC C  GFV  ++    + D+   +V         ++T+ S     ++  ++  D  KN +   +  SLA
Sbjct: 1709 GFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESP-CQQNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD---CMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQ-PCGNG-TCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFELTADG-KNCVDTNECLSLA 1937          

HSP 10 Score: 91.2781 bits (225), Expect = 1.578e-17
Identity = 97/289 (33.56%), Postives = 129/289 (44.64%), Query Frame = 3
Query: 2607 NCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECRDSKI---------CS---GNG------IC-QNMIGSYRCACHNGYEYVE--DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C+D +EC +L         C+NT GSF C C +  E            + C    + +S+  A TC  P  C +  G   C+CP GF     + CIDI+EC+  P  CL   TC NS GSF C CP GF            + S  F   +  +C   K          CS   G G      +C Q    +++  C  G+  V   D    D++EC  +  +C   N VC NT GSF+C CP G  L++    CVD DEC   + C +G TC N  GGF C C  GF
Sbjct: 1883 DCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFEL-------TADGKNCVDTNECLSL--AGTCL-PGTCQNLEGSFRCICPPGFQVQ-SDHCIDIDECSEEPNLCL-FGTCTNSPGSFQCLCPPGFVLSDNGHRCFDTRQSFCFTRFEAGKCSVPKAFNTTKTRCCCSKRPGEGWGDPCELCPQEGSAAFQELCPFGHGAVPGPDDSREDVNECAENPGVCT--NGVCVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQG-TCTNVIGGFECACADGF 2156          

HSP 11 Score: 89.3521 bits (220), Expect = 6.764e-17
Identity = 92/307 (29.97%), Postives = 127/307 (41.37%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDL-KEGCIDIN-ECNMNPCLDKATCRNSY------------------------------GSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            G NC+D NEC +     N  C+N  GSF C C        P F  AP    C          D   C++P      +C+++ G   C C  G         C+D +        ++K +C   +                                F   C  G   +      DI+EC  D ++C+ NG+C+N+ GSYRC C+ GYE     K C D+DEC  +  LC  DN  CQN+PGS+ C CP G  + +    C D+DEC  ++ C  G  C N  G + C C  G   +    FC LD T
Sbjct:  526 GKNCVDHNECATSTMCVNGVCLNEDGSFSCLC-------KPGFLLAPGGHYCM---------DIDECQTPGICVNGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG--RDINECALDPEVCA-NGVCENLRGSYRCVCNLGYEAGASGKDCTDVDECALNSLLC--DNGWCQNSPGSYSCSCPPGFHFWQDTEICKDVDEC-LSSPCVSG-VCRNLAGSYTCKCGPGSRLDPSGTFC-LDST 808          

HSP 12 Score: 88.9669 bits (219), Expect = 8.208e-17
Identity = 62/168 (36.90%), Postives = 84/168 (50.00%), Query Frame = 3
Query: 2889 IDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            IDI     N CL+   C  +  S+ C C  G+   V   C+D+DEC  S     +G C N+ G+Y C C+ G++    +  C+D+DEC  S  LC     V  NT GSF+CVC  G E    G  CVD +EC  +  C  G  C+N  G F+C C  GF+      +C
Sbjct:  409 IDICRHFTNLCLN-GRCLPTPSSYRCECNVGYTQDVRGECIDVDECTSSPC--HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCV--NTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNG-VCLNEDGSFSCLCKPGFLLAPGGHYC 570          

HSP 13 Score: 87.4261 bits (215), Expect = 2.369e-16
Identity = 73/214 (34.11%), Postives = 98/214 (45.79%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKI-CSGNGI-CQNMIGSYRCACHNGYEYV--EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            D+NEC ++     N  C+NT GSF C C            C    +ECS                   C++  G   C C  GF   L   C DI+EC++NP L    C N+ GS+ C CP G+        C D+DEC D +  C   G+ C+N+IG++ C C  G   +    +GC D +EC A   LC   N  C NT GSF+C C  G +
Sbjct: 2084 DVNECAENPGVCTNGVCVNTDGSFRCECPFGYSLDFTGINCV-DTDECSVGHPC----------GQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCV--NGRCVNTAGSFRCDCDEGFQ 2284          

HSP 14 Score: 85.1149 bits (209), Expect = 1.340e-15
Identity = 96/341 (28.15%), Postives = 131/341 (38.42%), Query Frame = 3
Query: 2460 TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            T+N  +DI R    L +       + L       C+   C  GY Q +   CID++EC S  CH+   C+N PG++ C C    +       C   ++EC     +  +           C ++ G   CVC  GF      + C+D NEC  +       C N  GSF C C  GF  +   H+ C+DIDEC+   IC  NG C N  GS+RC C  G                   Y  +E   C        +K  C       G    CQ  P      F+ +C  GL     G  DI+EC  +        C N  G + C CN G+      K C
Sbjct:  404 TLNQTIDICRHFTNLCLN-----GRCLPTPSSYRCE---CNVGYTQDVRGECIDVDECTSSPCHHGD-CVNIPGTYHCRCYPGFQATPTRQACV-DVDECIVSGGLCHL---------GRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNGVCLNEDGSFSCLCKPGFLLAPGGHY-CMDIDECQTPGICV-NGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG-RDINECALDPEVCANGVCENLRGSYRCVCNLGYEAGASGKDC 722          

HSP 15 Score: 80.8777 bits (198), Expect = 2.503e-14
Identity = 91/298 (30.54%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2616 DINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CP---------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L   C     C+NT GSF C C     +L+        I+ECS    I           P  C ++ G   CVCP  +   +    C+D+              NE   N  + +  C  SY     +      CP                GF   +H G  +DIDEC +      NGIC N IGS+RC C  G+ Y      C D+DEC + +  CQ  N  C N PGS++C C  G +    G CV  +EC++  N C  G  C+++ G + C C++GF  + D   C
Sbjct: 1563 DIDECQELPGLCQGGD-CVNTFGSFQCECPPGY-HLSEHTRICEDIDECSTHSGICG---------PGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFN--VTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESPCQ-QNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD-CMDTEGSYMCLCHRGFQASADQTLC 1845          

HSP 16 Score: 80.8777 bits (198), Expect = 2.655e-14
Identity = 71/235 (30.21%), Postives = 102/235 (43.40%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK-----------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNP-CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + +   RT K+L +  S+           V   TC+   C  G+ +    C D +EC +    C  +  C NTPGSF C C      ++   GC           + ++ CD    R    C +  G   C CP+GFT +     C+D NEC ++P     A+CRN+ G F C CP GF +    G C ++DEC   +  CS +  C N  G + C C  GY       C+
Sbjct: 2432 PRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCR---CPPGFTQHHQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGC-----------EDVNECDGPH-RCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPCSYS--CANTPGGFLCGCPQGYFRAGQGHCV 2649          

HSP 17 Score: 80.1073 bits (196), Expect = 3.812e-14
Identity = 106/337 (31.45%), Postives = 143/337 (42.43%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERK-------------IISICDAVTC----RSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG---CIDIDE----------CQ------ASKKLC-------QGDNVVCQNTPGS----FKCVC----PVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+  + G NC DINEC D + C N   CINTPGS++C C  + E LNP   GC   +   C  E                + +  A  C    R+  N    C  +N  E+  +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  S H   C DIDEC       G G C N +G+Y C C    EY++  G   C+D+ +          CQ       ++K+C       Q  N  C+  P      ++ +C    P  L     G  +DIDEC +         CIN  G F C C  GF +N+    CE
Sbjct: 1430 CNGGYELDRGGGNCTDINECADPVNCINGV-CINTPGSYLCSCPQDFE-LNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICEDIDECSTHSGICGPGTCYNTLGNYTCVCP--AEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACE 1762          

HSP 18 Score: 77.7962 bits (190), Expect = 1.999e-13
Identity = 84/310 (27.10%), Postives = 126/310 (40.65%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID---------------------------IDECQASKKLCQGDNVVCQNTPGS----FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G  C D++EC S    C     C+N PGS+ C C   + +    F C  + +EC+   + + +CD         C ++ G   C C  GF   +    C D++EC         +C N  G F C C  G++     G C DI+EC D   C  NG+C N  GSY C+C   +E      GC+D                           +        L +     C+  P +    ++ +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  +  T+ CE
Sbjct: 1308 CLPGWVGDGFECHDLDECVSQEHRCSPRGDCLNVPGSYRCTC--RQGFAGDGFFCEDR-DECA---ENVDLCDN------GQCLNAPGGYRCECEMGFDPTEDHRACQDVDECAQGNLCAFGSCENLPGMFRCICNGGYELDRGGGNCTDINECADPVNCI-NGVCINTPGSYLCSCPQDFELNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICE 1604          

HSP 19 Score: 72.0182 bits (175), Expect = 1.263e-11
Identity = 86/317 (27.13%), Postives = 128/317 (40.38%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEE---YLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINEC--NMNPCLDKAT-CRNSYGSFDCFCPKGFQ--YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQN-----------------------------TPG--SFKCVCPVGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G NC+D +EC          C N  G F C C++  E    +  E      I+ECS    + +            C ++ G   C CP G+T  L+E    C D++EC      C  +   C+N  G+F C CP G +       GC D +EC        NG C N  GS+RC C  G++       C DI +     ++ Q    +C++                              PG  +++ +CP G  Y  +G  D+DEC+   + C  G  CINS G F C C  G+  +     C ++D+   V +P
Sbjct: 2121 GINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCE-----DIDECSLNPLLCAF----------RCHNTEGSYLCTCPAGYT--LREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQ---TMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPGTSAYRKLCPHGSGYTAEG-RDVDECRMLAHLCAHGE-CINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKP 2415          

HSP 20 Score: 65.4698 bits (158), Expect = 1.145e-9
Identity = 89/305 (29.18%), Postives = 126/305 (41.31%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY--DLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDE-----------CRD------------------------SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            C DI+EC         RC NT GS++C C      L  +      ++EC+  ++    C A        C +  G   CVCP G        EGC D NEC+  P  C++   C N+ GSF C C +GFQ S     C DI +           CR                          ++C   G       +YR  C +G  Y  +    D+DEC+    LC   +  C N+ GSF+C C  G   +     C+D+DEC Q    P    C N+ G F C+C +G++   D + C +LD+
Sbjct: 2164 CEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYT-LREDGAMCRDVDECADGQQD---CHARG----MECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVN-GRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPG-----TSAYRKLCPHGSGYTAEG--RDVDECRMLAHLCA--HGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPK-PCTFLCKNTKGSFLCSCPRGYLLEEDGRTCKDLDE 2449          

HSP 21 Score: 53.9138 bits (128), Expect = 3.426e-6
Identity = 85/326 (26.07%), Postives = 128/326 (39.26%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRN--------SYGS------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLCQGD-----NVVCQNTPGS----FKCVCPVGLEYKEKGCV-------DIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G +C D++EC  +SL C +N  C N+PGS+ C C     +      C   ++EC     +  +C     + TC+            FC+     T  LK   I  + C +N  L  A+ R+        ++GS       D  C +GF       C D++EC        NG C N  GS+RC C  G       + C+D+           DEC  +        +C         V C+  P      F  +CP GL +  +  +       D++EC+     C  G TC N+ G F+C C  GF  +   + C
Sbjct:  709 CNLGYEAGASGKDCTDVDECALNSLLC-DNGWCQNSPGSYSCSCPPGFHFWQDTEICK-DVDECLSSPCVSGVCRNLAGSYTCKCGPGSRLDPSGTFCLDSTKGTCWLK---IQESRCEVN--LQGASLRSECCATLGAAWGSPCERCEIDPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCFLRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEACPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHG-TCRNTVGSFHCACAGGFALDAQERNC 1026          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Match: fbn-1 (FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7LFE6])

HSP 1 Score: 117.857 bits (294), Expect = 5.956e-26
Identity = 101/335 (30.15%), Postives = 142/335 (42.39%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSN----------GVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGC----------------------------------------------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  G++K  D +C DI+EC   +S  C  N++C+N PG++ C C N   +L   + C P  ++          CD+ T  S ++CS SN           VE   C  G+    K  C DINEC     PC   A C N  G+F C C +G++    F C DI+EC +   C  +  C N+ GS++C CH+G+E    K C                                                    +DIDEC+ S+  C   + VC NT GS++C C  G E +   C DIDEC +    C   + CIN  G   C C  G+  +  T
Sbjct: 1459 CPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENG--FLGDGYQCVPTTKK---------PCDS-TQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKV-CEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGYRGD-GFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPASAVCVNTEGSYRCECAEGYEGEGGVCTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGYTGDGAT 1779          

HSP 2 Score: 93.9745 bits (232), Expect = 8.772e-19
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKG-----CIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKGCVDIDEC--QQNNTCPEGSTCINSFGGF 3332
            TC     C        C C  GF+    E   D NEC  +PC   A C+N+ GS++C C  G++ + H  C DIDEC D  +  C  +  C N+ G+Y C C  G+    ++G     C DI+EC+     C  D  +C+N+ GSFKCV  C  G E  +  C D++EC  ++ + C   + C+N+ GG+
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFSGSACE-LQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE-CTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADE-ICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGY 288          

HSP 3 Score: 89.7373 bits (221), Expect = 1.929e-17
Identity = 100/327 (30.58%), Postives = 130/327 (39.76%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPA--NCSSSNGVEFCVCPRGFTYDLKE-GCIDINEC-----NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDE----------------CQASKKLCQGD-----------------NVVCQN----------------------TPGSFKCVCPVGLEYKEKGCVDIDECQQNNTC-PEGSTCINSFGGFNCTCNKGF 3356
            +C +GY+  G  C DINECD    CH ++ C N  GSF C C +  E    +    P    C    K     D V+C S    N S S+    C C  GF ++ +   C+DI+EC     N +P    A C N+ GS+ C C +G++      C DIDEC R    C    +C N +GS  C C  GY   +   CI I+E                C+  KK C  D                 N  CQ+                       P S  C CP G       C D+DEC     C  E + C N+ G FNC C +GF
Sbjct: 1592 SCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSS---VCECEPGFRFEKESNSCVDIDECEESRNNCDPA--SAVCVNTEGSYRCECAEGYEGEGGV-CTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGY-TGDGATCIKIEEEPKSDKTACTDEWSRLCELEKKQCTVDEEEVPQCGACLPGHHPINGTCQSLQISGLCAQKNDCNKHAECIDIHPDSHFCSCPDGFIGDGMICDDVDECNNAGMCDDENTKCENTIGSFNCVCLEGF 1911          

HSP 4 Score: 75.8702 bits (185), Expect = 2.795e-13
Identity = 73/227 (32.16%), Postives = 95/227 (41.85%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISICDAVT----CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQ--NMIGSYRCA-CHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C P  E    ++  + + +  T    C   A C +  G   C CP GF       C DI+EC   N   C   A C N  G++ C C  GF     + CV       D  +   S     N  C+   + GS  C  C  GY+    K C DI+EC A K  C   N  C N  G+F C C  G  Y+  G  C DI+EC + + C   + C N  G F C C+ GF
Sbjct: 1416 CEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGF-LGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKK-SGKVCEDINECVAEKAPCS-LNANCVNMNGTFSCSCKQG--YRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGF 1637          

HSP 5 Score: 72.0182 bits (175), Expect = 4.168e-12
Identity = 92/310 (29.68%), Postives = 132/310 (42.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD--AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN---MNPCLDKATCRNSYGSFD---------------------------------------CFCPKGFQYSVHFGCVDIDECRDSK--ICSGNGICQNMIGSYRCACHNGYEY---VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY-KEKGCVDIDECQQNNTC--PEGSTCINSFGGFNC 3338
            C  GY+  G  C DI+EC D L   C  +S+CIN PG++ C C+   +   P+      +++C+     I+ C+  A  C +   C +S G   CV      Y+L +G C D+NEC    ++ C  +A C N+ G ++                                       C C  G+       C DI+EC D+K   CS  G C N+ G Y C C NG +    ++D+G      C        GDN +C N      C C  G      K CVDI+EC +N++     G  C+N FGGF C
Sbjct:  159 CLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCT---QGFTPKGNQGSGLDKCAD----INECETGAHNCDADEICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGYECECEEGFEGDGKNCQPKSSCRKNSAICDRHASCHIVLDICDCKTGYTGD-GITCHDINEC-DAKDTPCSDGGRCLNLDGGYVC-CKNGQDDATCIKDQGAFCSGGC--------GDNAICSNA----TCACIDGFRGDPHKKCVDINECVENDSVCGGVGDRCVNLFGGFKC 446          

HSP 6 Score: 69.707 bits (169), Expect = 2.063e-11
Identity = 58/210 (27.62%), Postives = 79/210 (37.62%), Query Frame = 3
Query: 2886 CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL------------------------------------------------EYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C   +EC ++     A C    G   C C  GF+ +     CVD+DEC      C  +  CQN +G Y C C  G+   +D  C DIDEC      C G N  C N PG++ C C  G                                                  YK+ G  C DI+EC  +   C   + C+N  G F+C+C +G+
Sbjct: 1395 CTSSDECGLD-----ALCERRTGV--CRCEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKVCEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGY 1597          

HSP 7 Score: 68.5514 bits (166), Expect = 4.867e-11
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 3
Query: 3009 VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFNN 3368
            V+  +C  S  C  + +C+   G   C C  G+E    K  C+D+DEC      C  ++  CQN  G + C CP G    + G C DIDEC ++N+  C   + C+N  G ++C C  GF+ + 
Sbjct: 1390 VENTKCTSSDECGLDALCERRTG--VCRCEPGFEGAPPKKSCVDVDECATGDHNCH-ESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDG 1510          

HSP 8 Score: 53.5286 bits (127), Expect = 1.830e-6
Identity = 40/109 (36.70%), Postives = 50/109 (45.87%), Query Frame = 3
Query: 3039 ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
             CSG G C       +C C +G+       C   D+ +  +  C      CQNT GS++C C  G E     C DIDEC    T  CPE S CIN  G + C C +GF 
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFS---GSACELQDKNECLEHPCH-MMAQCQNTLGSYECRCLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFT 206          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Match: fbn-1 (FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7LFE6])

HSP 1 Score: 116.701 bits (291), Expect = 1.124e-25
Identity = 101/335 (30.15%), Postives = 142/335 (42.39%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSN----------GVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGC----------------------------------------------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  G++K  D +C DI+EC   +S  C  N++C+N PG++ C C N   +L   + C P  ++          CD+ T  S ++CS SN           VE   C  G+    K  C DINEC     PC   A C N  G+F C C +G++    F C DI+EC +   C  +  C N+ GS++C CH+G+E    K C                                                    +DIDEC+ S+  C   + VC NT GS++C C  G E +   C DIDEC +    C   + CIN  G   C C  G+  +  T
Sbjct: 1459 CPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENG--FLGDGYQCVPTTKK---------PCDS-TQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKV-CEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGYRGD-GFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPASAVCVNTEGSYRCECAEGYEGEGGVCTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGYTGDGAT 1779          

HSP 2 Score: 93.9745 bits (232), Expect = 1.105e-18
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKG-----CIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKGCVDIDEC--QQNNTCPEGSTCINSFGGF 3332
            TC     C        C C  GF+    E   D NEC  +PC   A C+N+ GS++C C  G++ + H  C DIDEC D  +  C  +  C N+ G+Y C C  G+    ++G     C DI+EC+     C  D  +C+N+ GSFKCV  C  G E  +  C D++EC  ++ + C   + C+N+ GG+
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFSGSACE-LQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE-CTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADE-ICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGY 288          

HSP 3 Score: 90.1225 bits (222), Expect = 1.688e-17
Identity = 97/325 (29.85%), Postives = 128/325 (39.38%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE-GCIDINEC-----NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDE----------------CQASKKLCQGD-----------------NVVCQNT----------------------PGSFKCVCPVGLEYKEKGCVDIDECQQNNTC-PEGSTCINSFGGFNCTCNKGF 3356
            +C +GY+  G  C DINECD    CH ++ C N  GSF C C +  E    +    P    C    K     D V+C S     + +    C C  GF ++ +   C+DI+EC     N +P    A C N+ GS+ C C +G++      C DIDEC R    C    +C N +GS  C C  GY   +   CI I+E                C+  KK C  D                 N  CQ+                       P S  C CP G       C D+DEC     C  E + C N+ G FNC C +GF
Sbjct: 1592 SCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLS-VRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPA--SAVCVNTEGSYRCECAEGYEGEGGV-CTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGY-TGDGATCIKIEEEPKSDKTACTDEWSRLCELEKKQCTVDEEEVPQCGACLPGHHPINGTCQSLQISGLCAQKNDCNKHAECIDIHPDSHFCSCPDGFIGDGMICDDVDECNNAGMCDDENTKCENTIGSFNCVCLEGF 1911          

HSP 4 Score: 75.8702 bits (185), Expect = 3.256e-13
Identity = 71/225 (31.56%), Postives = 92/225 (40.89%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISICDAVT----CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQ--NMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C P  E    ++  + + +  T    C   A C +  G   C CP GF       C DI+EC   N   C   A C N  G++ C C  GF     + CV       D  +   S     N  C+   + GS  C  C  GY+    K C DI+EC A K  C   N  C N  G+F C C  G       C DI+EC + + C   + C N  G F C C+ GF
Sbjct: 1416 CEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGF-LGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKK-SGKVCEDINECVAEKAPCS-LNANCVNMNGTFSCSCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGF 1637          

HSP 5 Score: 72.0182 bits (175), Expect = 5.093e-12
Identity = 92/310 (29.68%), Postives = 132/310 (42.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD--AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN---MNPCLDKATCRNSYGSFD---------------------------------------CFCPKGFQYSVHFGCVDIDECRDSK--ICSGNGICQNMIGSYRCACHNGYEY---VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY-KEKGCVDIDECQQNNTC--PEGSTCINSFGGFNC 3338
            C  GY+  G  C DI+EC D L   C  +S+CIN PG++ C C+   +   P+      +++C+     I+ C+  A  C +   C +S G   CV      Y+L +G C D+NEC    ++ C  +A C N+ G ++                                       C C  G+       C DI+EC D+K   CS  G C N+ G Y C C NG +    ++D+G      C        GDN +C N      C C  G      K CVDI+EC +N++     G  C+N FGGF C
Sbjct:  159 CLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCT---QGFTPKGNQGSGLDKCA----DINECETGAHNCDADEICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGYECECEEGFEGDGKNCQPKSSCRKNSAICDRHASCHIVLDICDCKTGYTGD-GITCHDINEC-DAKDTPCSDGGRCLNLDGGYVC-CKNGQDDATCIKDQGAFCSGGC--------GDNAICSNA----TCACIDGFRGDPHKKCVDINECVENDSVCGGVGDRCVNLFGGFKC 446          

HSP 6 Score: 71.2478 bits (173), Expect = 7.011e-12
Identity = 83/272 (30.51%), Postives = 110/272 (40.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP--ANCSSSNGVEFCVCPRGFTYDLKEG-----CIDINECNMNP--CLDKATCRNSYGSFDCF--CPKGFQYSVHFGCVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSF----KCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNC 3338
            C  G+        D NEC    CH  ++C NT GS+ C C    E    E      I+ECS         D +T R P  + C +  G  +C C +GFT    +G     C DINEC      C     C NS GSF C   C  G++  +   C D++EC   K+  C     C N IG Y           E+    D   CQ  K  C+ ++ +C            C C  G       C DI+EC   +T C +G  C+N  GG+ C
Sbjct:  120 CDSGFSGSACELQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE---CTDIDECS---------DKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADEICENSIGSFKCVNKCSPGYEL-IDGKCEDVNECGSEKLHKCDVRADCVNTIGGY-------ECECEEGFEGDGKNCQP-KSSCRKNSAICDRHASCHIVLDICDCKTGYTGDGITCHDINECDAKDTPCSDGGRCLNLDGGYVC 370          

HSP 7 Score: 69.3218 bits (168), Expect = 3.350e-11
Identity = 58/210 (27.62%), Postives = 79/210 (37.62%), Query Frame = 3
Query: 2886 CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL------------------------------------------------EYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C   +EC ++     A C    G   C C  GF+ +     CVD+DEC      C  +  CQN +G Y C C  G+   +D  C DIDEC      C G N  C N PG++ C C  G                                                  YK+ G  C DI+EC  +   C   + C+N  G F+C+C +G+
Sbjct: 1395 CTSSDECGLD-----ALCERRTGV--CRCEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKVCEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGY 1597          

HSP 8 Score: 68.5514 bits (166), Expect = 5.935e-11
Identity = 41/121 (33.88%), Postives = 61/121 (50.41%), Query Frame = 3
Query: 3009 VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
            V+  +C  S  C  + +C+   G   C C  G+E    K  C+D+DEC      C  ++  CQN  G + C CP G    + G C DIDEC ++N+  C   + C+N  G ++C C  GF+
Sbjct: 1390 VENTKCTSSDECGLDALCERRTG--VCRCEPGFEGAPPKKSCVDVDECATGDHNCH-ESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFL 1507          

HSP 9 Score: 53.9138 bits (128), Expect = 1.621e-6
Identity = 40/109 (36.70%), Postives = 50/109 (45.87%), Query Frame = 3
Query: 3039 ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
             CSG G C       +C C +G+       C   D+ +  +  C      CQNT GS++C C  G E     C DIDEC    T  CPE S CIN  G + C C +GF 
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFS---GSACELQDKNECLEHPCH-MMAQCQNTLGSYECRCLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFT 206          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Match: fbn-1 (FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7LFE6])

HSP 1 Score: 116.701 bits (291), Expect = 1.202e-25
Identity = 101/335 (30.15%), Postives = 142/335 (42.39%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSN----------GVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGC----------------------------------------------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  G++K  D +C DI+EC   +S  C  N++C+N PG++ C C N   +L   + C P  ++          CD+ T  S ++CS SN           VE   C  G+    K  C DINEC     PC   A C N  G+F C C +G++    F C DI+EC +   C  +  C N+ GS++C CH+G+E    K C                                                    +DIDEC+ S+  C   + VC NT GS++C C  G E +   C DIDEC +    C   + CIN  G   C C  G+  +  T
Sbjct: 1461 CPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENG--FLGDGYQCVPTTKK---------PCDS-TQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKV-CEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGYRGD-GFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPASAVCVNTEGSYRCECAEGYEGEGGVCTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGYTGDGAT 1781          

HSP 2 Score: 93.9745 bits (232), Expect = 1.078e-18
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKG-----CIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKGCVDIDEC--QQNNTCPEGSTCINSFGGF 3332
            TC     C        C C  GF+    E   D NEC  +PC   A C+N+ GS++C C  G++ + H  C DIDEC D  +  C  +  C N+ G+Y C C  G+    ++G     C DI+EC+     C  D  +C+N+ GSFKCV  C  G E  +  C D++EC  ++ + C   + C+N+ GG+
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFSGSACE-LQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE-CTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADE-ICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGY 288          

HSP 3 Score: 89.7373 bits (221), Expect = 1.702e-17
Identity = 97/325 (29.85%), Postives = 128/325 (39.38%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE-GCIDINEC-----NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDE----------------CQASKKLCQGD-----------------NVVCQNT----------------------PGSFKCVCPVGLEYKEKGCVDIDECQQNNTC-PEGSTCINSFGGFNCTCNKGF 3356
            +C +GY+  G  C DINECD    CH ++ C N  GSF C C +  E    +    P    C    K     D V+C S     + +    C C  GF ++ +   C+DI+EC     N +P    A C N+ GS+ C C +G++      C DIDEC R    C    +C N +GS  C C  GY   +   CI I+E                C+  KK C  D                 N  CQ+                       P S  C CP G       C D+DEC     C  E + C N+ G FNC C +GF
Sbjct: 1594 SCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLS-VRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPA--SAVCVNTEGSYRCECAEGYEGEGGV-CTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGY-TGDGATCIKIEEEPKSDKTACTDEWSRLCELEKKQCTVDEEEVPQCGACLPGHHPINGTCQSLQISGLCAQKNDCNKHAECIDIHPDSHFCSCPDGFIGDGMICDDVDECNNAGMCDDENTKCENTIGSFNCVCLEGF 1913          

HSP 4 Score: 75.8702 bits (185), Expect = 3.229e-13
Identity = 71/225 (31.56%), Postives = 92/225 (40.89%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISICDAVT----CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQ--NMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C P  E    ++  + + +  T    C   A C +  G   C CP GF       C DI+EC   N   C   A C N  G++ C C  GF     + CV       D  +   S     N  C+   + GS  C  C  GY+    K C DI+EC A K  C   N  C N  G+F C C  G       C DI+EC + + C   + C N  G F C C+ GF
Sbjct: 1418 CEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGF-LGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKK-SGKVCEDINECVAEKAPCS-LNANCVNMNGTFSCSCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGF 1639          

HSP 5 Score: 72.0182 bits (175), Expect = 4.967e-12
Identity = 92/310 (29.68%), Postives = 132/310 (42.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD--AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN---MNPCLDKATCRNSYGSFD---------------------------------------CFCPKGFQYSVHFGCVDIDECRDSK--ICSGNGICQNMIGSYRCACHNGYEY---VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY-KEKGCVDIDECQQNNTC--PEGSTCINSFGGFNC 3338
            C  GY+  G  C DI+EC D L   C  +S+CIN PG++ C C+   +   P+      +++C+     I+ C+  A  C +   C +S G   CV      Y+L +G C D+NEC    ++ C  +A C N+ G ++                                       C C  G+       C DI+EC D+K   CS  G C N+ G Y C C NG +    ++D+G      C        GDN +C N      C C  G      K CVDI+EC +N++     G  C+N FGGF C
Sbjct:  159 CLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCT---QGFTPKGNQGSGLDKCA----DINECETGAHNCDADEICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGYECECEEGFEGDGKNCQPKSSCRKNSAICDRHASCHIVLDICDCKTGYTGD-GITCHDINEC-DAKDTPCSDGGRCLNLDGGYVC-CKNGQDDATCIKDQGAFCSGGC--------GDNAICSNA----TCACIDGFRGDPHKKCVDINECVENDSVCGGVGDRCVNLFGGFKC 446          

HSP 6 Score: 71.633 bits (174), Expect = 6.780e-12
Identity = 83/272 (30.51%), Postives = 110/272 (40.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP--ANCSSSNGVEFCVCPRGFTYDLKEG-----CIDINECNMNP--CLDKATCRNSYGSFDCF--CPKGFQYSVHFGCVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSF----KCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNC 3338
            C  G+        D NEC    CH  ++C NT GS+ C C    E    E      I+ECS         D +T R P  + C +  G  +C C +GFT    +G     C DINEC      C     C NS GSF C   C  G++  +   C D++EC   K+  C     C N IG Y           E+    D   CQ  K  C+ ++ +C            C C  G       C DI+EC   +T C +G  C+N  GG+ C
Sbjct:  120 CDSGFSGSACELQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE---CTDIDECS---------DKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADEICENSIGSFKCVNKCSPGYEL-IDGKCEDVNECGSEKLHKCDVRADCVNTIGGY-------ECECEEGFEGDGKNCQP-KSSCRKNSAICDRHASCHIVLDICDCKTGYTGDGITCHDINECDAKDTPCSDGGRCLNLDGGYVC 370          

HSP 7 Score: 69.3218 bits (168), Expect = 3.322e-11
Identity = 58/210 (27.62%), Postives = 79/210 (37.62%), Query Frame = 3
Query: 2886 CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL------------------------------------------------EYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C   +EC ++     A C    G   C C  GF+ +     CVD+DEC      C  +  CQN +G Y C C  G+   +D  C DIDEC      C G N  C N PG++ C C  G                                                  YK+ G  C DI+EC  +   C   + C+N  G F+C+C +G+
Sbjct: 1397 CTSSDECGLD-----ALCERRTGV--CRCEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKVCEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGY 1599          

HSP 8 Score: 68.5514 bits (166), Expect = 5.836e-11
Identity = 41/121 (33.88%), Postives = 61/121 (50.41%), Query Frame = 3
Query: 3009 VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
            V+  +C  S  C  + +C+   G   C C  G+E    K  C+D+DEC      C  ++  CQN  G + C CP G    + G C DIDEC ++N+  C   + C+N  G ++C C  GF+
Sbjct: 1392 VENTKCTSSDECGLDALCERRTG--VCRCEPGFEGAPPKKSCVDVDECATGDHNCH-ESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFL 1509          

HSP 9 Score: 53.9138 bits (128), Expect = 1.594e-6
Identity = 40/109 (36.70%), Postives = 50/109 (45.87%), Query Frame = 3
Query: 3039 ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
             CSG G C       +C C +G+       C   D+ +  +  C      CQNT GS++C C  G E     C DIDEC    T  CPE S CIN  G + C C +GF 
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFS---GSACELQDKNECLEHPCH-MMAQCQNTLGSYECRCLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFT 206          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Match: fbn-1 (FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7LFE6])

HSP 1 Score: 116.316 bits (290), Expect = 1.435e-25
Identity = 101/335 (30.15%), Postives = 142/335 (42.39%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSN----------GVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGC----------------------------------------------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  G++K  D +C DI+EC   +S  C  N++C+N PG++ C C N   +L   + C P  ++          CD+ T  S ++CS SN           VE   C  G+    K  C DINEC     PC   A C N  G+F C C +G++    F C DI+EC +   C  +  C N+ GS++C CH+G+E    K C                                                    +DIDEC+ S+  C   + VC NT GS++C C  G E +   C DIDEC +    C   + CIN  G   C C  G+  +  T
Sbjct: 1459 CPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENG--FLGDGYQCVPTTKK---------PCDS-TQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKV-CEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGYRGD-GFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPASAVCVNTEGSYRCECAEGYEGEGGVCTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGYTGDGAT 1779          

HSP 2 Score: 93.9745 bits (232), Expect = 1.117e-18
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKG-----CIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKGCVDIDEC--QQNNTCPEGSTCINSFGGF 3332
            TC     C        C C  GF+    E   D NEC  +PC   A C+N+ GS++C C  G++ + H  C DIDEC D  +  C  +  C N+ G+Y C C  G+    ++G     C DI+EC+     C  D  +C+N+ GSFKCV  C  G E  +  C D++EC  ++ + C   + C+N+ GG+
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFSGSACE-LQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE-CTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADE-ICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGY 288          

HSP 3 Score: 89.7373 bits (221), Expect = 1.970e-17
Identity = 97/325 (29.85%), Postives = 128/325 (39.38%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE-GCIDINEC-----NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDE----------------CQASKKLCQGD-----------------NVVCQNT----------------------PGSFKCVCPVGLEYKEKGCVDIDECQQNNTC-PEGSTCINSFGGFNCTCNKGF 3356
            +C +GY+  G  C DINECD    CH ++ C N  GSF C C +  E    +    P    C    K     D V+C S     + +    C C  GF ++ +   C+DI+EC     N +P    A C N+ GS+ C C +G++      C DIDEC R    C    +C N +GS  C C  GY   +   CI I+E                C+  KK C  D                 N  CQ+                       P S  C CP G       C D+DEC     C  E + C N+ G FNC C +GF
Sbjct: 1592 SCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLS-VRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPA--SAVCVNTEGSYRCECAEGYEGEGGV-CTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGY-TGDGATCIKIEEEPKSDKTACTDEWSRLCELEKKQCTVDEEEVPQCGACLPGHHPINGTCQSLQISGLCAQKNDCNKHAECIDIHPDSHFCSCPDGFIGDGMICDDVDECNNAGMCDDENTKCENTIGSFNCVCLEGF 1911          

HSP 4 Score: 75.8702 bits (185), Expect = 3.214e-13
Identity = 71/225 (31.56%), Postives = 92/225 (40.89%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISICDAVT----CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQ--NMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C P  E    ++  + + +  T    C   A C +  G   C CP GF       C DI+EC   N   C   A C N  G++ C C  GF     + CV       D  +   S     N  C+   + GS  C  C  GY+    K C DI+EC A K  C   N  C N  G+F C C  G       C DI+EC + + C   + C N  G F C C+ GF
Sbjct: 1416 CEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGF-LGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKK-SGKVCEDINECVAEKAPCS-LNANCVNMNGTFSCSCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGF 1637          

HSP 5 Score: 71.633 bits (174), Expect = 5.658e-12
Identity = 92/310 (29.68%), Postives = 132/310 (42.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD--AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN---MNPCLDKATCRNSYGSFD---------------------------------------CFCPKGFQYSVHFGCVDIDECRDSK--ICSGNGICQNMIGSYRCACHNGYEY---VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY-KEKGCVDIDECQQNNTC--PEGSTCINSFGGFNC 3338
            C  GY+  G  C DI+EC D L   C  +S+CIN PG++ C C+   +   P+      +++C+     I+ C+  A  C +   C +S G   CV      Y+L +G C D+NEC    ++ C  +A C N+ G ++                                       C C  G+       C DI+EC D+K   CS  G C N+ G Y C C NG +    ++D+G      C        GDN +C N      C C  G      K CVDI+EC +N++     G  C+N FGGF C
Sbjct:  159 CLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCT---QGFTPKGNQGSGLDKCA----DINECETGAHNCDADEICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGYECECEEGFEGDGKNCQPKSSCRKNSAICDRHASCHIVLDICDCKTGYTGD-GITCHDINEC-DAKDTPCSDGGRCLNLDGGYVC-CKNGQDDATCIKDQGAFCSGGC--------GDNAICSNA----TCACIDGFRGDPHKKCVDINECVENDSVCGGVGDRCVNLFGGFKC 446          

HSP 6 Score: 71.633 bits (174), Expect = 6.808e-12
Identity = 83/272 (30.51%), Postives = 110/272 (40.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP--ANCSSSNGVEFCVCPRGFTYDLKEG-----CIDINECNMNP--CLDKATCRNSYGSFDCF--CPKGFQYSVHFGCVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSF----KCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNC 3338
            C  G+        D NEC    CH  ++C NT GS+ C C    E    E      I+ECS         D +T R P  + C +  G  +C C +GFT    +G     C DINEC      C     C NS GSF C   C  G++  +   C D++EC   K+  C     C N IG Y           E+    D   CQ  K  C+ ++ +C            C C  G       C DI+EC   +T C +G  C+N  GG+ C
Sbjct:  120 CDSGFSGSACELQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE---CTDIDECS---------DKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADEICENSIGSFKCVNKCSPGYEL-IDGKCEDVNECGSEKLHKCDVRADCVNTIGGY-------ECECEEGFEGDGKNCQP-KSSCRKNSAICDRHASCHIVLDICDCKTGYTGDGITCHDINECDAKDTPCSDGGRCLNLDGGYVC 370          

HSP 7 Score: 68.9366 bits (167), Expect = 3.754e-11
Identity = 58/210 (27.62%), Postives = 79/210 (37.62%), Query Frame = 3
Query: 2886 CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL------------------------------------------------EYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C   +EC ++     A C    G   C C  GF+ +     CVD+DEC      C  +  CQN +G Y C C  G+   +D  C DIDEC      C G N  C N PG++ C C  G                                                  YK+ G  C DI+EC  +   C   + C+N  G F+C+C +G+
Sbjct: 1395 CTSSDECGLD-----ALCERRTGV--CRCEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKVCEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGY 1597          

HSP 8 Score: 68.1662 bits (165), Expect = 6.540e-11
Identity = 41/121 (33.88%), Postives = 61/121 (50.41%), Query Frame = 3
Query: 3009 VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
            V+  +C  S  C  + +C+   G   C C  G+E    K  C+D+DEC      C  ++  CQN  G + C CP G    + G C DIDEC ++N+  C   + C+N  G ++C C  GF+
Sbjct: 1390 VENTKCTSSDECGLDALCERRTG--VCRCEPGFEGAPPKKSCVDVDECATGDHNCH-ESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFL 1507          

HSP 9 Score: 53.5286 bits (127), Expect = 1.788e-6
Identity = 40/109 (36.70%), Postives = 50/109 (45.87%), Query Frame = 3
Query: 3039 ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
             CSG G C       +C C +G+       C   D+ +  +  C      CQNT GS++C C  G E     C DIDEC    T  CPE S CIN  G + C C +GF 
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFS---GSACELQDKNECLEHPCHM-MAQCQNTLGSYECRCLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFT 206          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Match: fbn-1 (FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7LFE6])

HSP 1 Score: 116.316 bits (290), Expect = 1.544e-25
Identity = 101/335 (30.15%), Postives = 142/335 (42.39%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSN----------GVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGC----------------------------------------------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  G++K  D +C DI+EC   +S  C  N++C+N PG++ C C N   +L   + C P  ++          CD+ T  S ++CS SN           VE   C  G+    K  C DINEC     PC   A C N  G+F C C +G++    F C DI+EC +   C  +  C N+ GS++C CH+G+E    K C                                                    +DIDEC+ S+  C   + VC NT GS++C C  G E +   C DIDEC +    C   + CIN  G   C C  G+  +  T
Sbjct: 1459 CPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENG--FLGDGYQCVPTTKK---------PCDS-TQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKV-CEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGYRGD-GFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSSVCECEPGFRFEKESNSCVDIDECEESRNNCDPASAVCVNTEGSYRCECAEGYEGEGGVCTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGYTGDGAT 1779          

HSP 2 Score: 93.5893 bits (231), Expect = 1.223e-18
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKG-----CIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKGCVDIDEC--QQNNTCPEGSTCINSFGGF 3332
            TC     C        C C  GF+    E   D NEC  +PC   A C+N+ GS++C C  G++ + H  C DIDEC D  +  C  +  C N+ G+Y C C  G+    ++G     C DI+EC+     C  D  +C+N+ GSFKCV  C  G E  +  C D++EC  ++ + C   + C+N+ GG+
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFSGSACE-LQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE-CTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADE-ICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGY 288          

HSP 3 Score: 89.7373 bits (221), Expect = 2.120e-17
Identity = 100/327 (30.58%), Postives = 130/327 (39.76%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPA--NCSSSNGVEFCVCPRGFTYDLKE-GCIDINEC-----NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDE----------------CQASKKLCQGD-----------------NVVCQNT----------------------PGSFKCVCPVGLEYKEKGCVDIDECQQNNTC-PEGSTCINSFGGFNCTCNKGF 3356
            +C +GY+  G  C DINECD    CH ++ C N  GSF C C +  E    +    P    C    K     D V+C S    N S S+    C C  GF ++ +   C+DI+EC     N +P    A C N+ GS+ C C +G++      C DIDEC R    C    +C N +GS  C C  GY   +   CI I+E                C+  KK C  D                 N  CQ+                       P S  C CP G       C D+DEC     C  E + C N+ G FNC C +GF
Sbjct: 1592 SCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGFEGDGIKKCTNPLERSCEDVEKFCGRVDHVSCLSVRIYNGSLSS---VCECEPGFRFEKESNSCVDIDECEESRNNCDPA--SAVCVNTEGSYRCECAEGYEGEGGV-CTDIDECDRGMAGCDSMAMCINRMGSCGCKCMAGY-TGDGATCIKIEEEPKSDKTACTDEWSRLCELEKKQCTVDEEEVPQCGACLPGHHPINGTCQSLQISGLCAQKNDCNKHAECIDIHPDSHFCSCPDGFIGDGMICDDVDECNNAGMCDDENTKCENTIGSFNCVCLEGF 1911          

HSP 4 Score: 75.8702 bits (185), Expect = 3.461e-13
Identity = 71/225 (31.56%), Postives = 92/225 (40.89%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISICDAVT----CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQ--NMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C P  E    ++  + + +  T    C   A C +  G   C CP GF       C DI+EC   N   C   A C N  G++ C C  GF     + CV       D  +   S     N  C+   + GS  C  C  GY+    K C DI+EC A K  C   N  C N  G+F C C  G       C DI+EC + + C   + C N  G F C C+ GF
Sbjct: 1416 CEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGF-LGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKK-SGKVCEDINECVAEKAPCS-LNANCVNMNGTFSCSCKQGYRGDGFMCTDINECDERHPCHPHAECTNLEGSFKCECHSGF 1637          

HSP 5 Score: 71.633 bits (174), Expect = 5.992e-12
Identity = 92/310 (29.68%), Postives = 132/310 (42.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD--AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN---MNPCLDKATCRNSYGSFD---------------------------------------CFCPKGFQYSVHFGCVDIDECRDSK--ICSGNGICQNMIGSYRCACHNGYEY---VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY-KEKGCVDIDECQQNNTC--PEGSTCINSFGGFNC 3338
            C  GY+  G  C DI+EC D L   C  +S+CIN PG++ C C+   +   P+      +++C+     I+ C+  A  C +   C +S G   CV      Y+L +G C D+NEC    ++ C  +A C N+ G ++                                       C C  G+       C DI+EC D+K   CS  G C N+ G Y C C NG +    ++D+G      C        GDN +C N      C C  G      K CVDI+EC +N++     G  C+N FGGF C
Sbjct:  159 CLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCT---QGFTPKGNQGSGLDKCA----DINECETGAHNCDADEICENSIGSFKCVNKCSPGYELIDGKCEDVNECGSEKLHKCDVRADCVNTIGGYECECEEGFEGDGKNCQPKSSCRKNSAICDRHASCHIVLDICDCKTGYTGD-GITCHDINEC-DAKDTPCSDGGRCLNLDGGYVC-CKNGQDDATCIKDQGAFCSGGC--------GDNAICSNA----TCACIDGFRGDPHKKCVDINECVENDSVCGGVGDRCVNLFGGFKC 446          

HSP 6 Score: 71.2478 bits (173), Expect = 7.031e-12
Identity = 83/272 (30.51%), Postives = 110/272 (40.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP--ANCSSSNGVEFCVCPRGFTYDLKEG-----CIDINECNMNP--CLDKATCRNSYGSFDCF--CPKGFQYSVHFGCVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSF----KCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNC 3338
            C  G+        D NEC    CH  ++C NT GS+ C C    E    E      I+ECS         D +T R P  + C +  G  +C C +GFT    +G     C DINEC      C     C NS GSF C   C  G++  +   C D++EC   K+  C     C N IG Y           E+    D   CQ  K  C+ ++ +C            C C  G       C DI+EC   +T C +G  C+N  GG+ C
Sbjct:  120 CDSGFSGSACELQDKNECLEHPCHMMAQCQNTLGSYECRCLPGYEGNGHE---CTDIDECS---------DKLTSRCPEHSKCINLPGTYYCNCTQGFTPKGNQGSGLDKCADINECETGAHNCDADEICENSIGSFKCVNKCSPGYEL-IDGKCEDVNECGSEKLHKCDVRADCVNTIGGY-------ECECEEGFEGDGKNCQP-KSSCRKNSAICDRHASCHIVLDICDCKTGYTGDGITCHDINECDAKDTPCSDGGRCLNLDGGYVC 370          

HSP 7 Score: 68.9366 bits (167), Expect = 4.361e-11
Identity = 58/210 (27.62%), Postives = 79/210 (37.62%), Query Frame = 3
Query: 2886 CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL------------------------------------------------EYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C   +EC ++     A C    G   C C  GF+ +     CVD+DEC      C  +  CQN +G Y C C  G+   +D  C DIDEC      C G N  C N PG++ C C  G                                                  YK+ G  C DI+EC  +   C   + C+N  G F+C+C +G+
Sbjct: 1395 CTSSDECGLD-----ALCERRTGV--CRCEPGFEGAPPKKSCVDVDECATGDHNCHESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFLGDGYQCVPTTKKPCDSTQSSKSHCSESNMSCEVDTVDGSVECKECMGGYKKSGKVCEDINECVAEKAPCSLNANCVNMNGTFSCSCKQGY 1597          

HSP 8 Score: 68.1662 bits (165), Expect = 7.224e-11
Identity = 41/121 (33.88%), Postives = 61/121 (50.41%), Query Frame = 3
Query: 3009 VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
            V+  +C  S  C  + +C+   G   C C  G+E    K  C+D+DEC      C  ++  CQN  G + C CP G    + G C DIDEC ++N+  C   + C+N  G ++C C  GF+
Sbjct: 1390 VENTKCTSSDECGLDALCERRTG--VCRCEPGFEGAPPKKSCVDVDECATGDHNCH-ESARCQNYVGGYACFCPTGFRKADDGSCQDIDECTEHNSTCCGANAKCVNKPGTYSCECENGFL 1507          

HSP 9 Score: 53.5286 bits (127), Expect = 1.911e-6
Identity = 40/109 (36.70%), Postives = 50/109 (45.87%), Query Frame = 3
Query: 3039 ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFV 3359
             CSG G C       +C C +G+       C   D+ +  +  C      CQNT GS++C C  G E     C DIDEC    T  CPE S CIN  G + C C +GF 
Sbjct:  102 TCSGKGACLYDGAKPQCYCDSGFS---GSACELQDKNECLEHPCHM-MAQCQNTLGSYECRCLPGYEGNGHECTDIDECSDKLTSRCPEHSKCINLPGTYYCNCTQGFT 206          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Match: dpy (gene:FBgn0053196 transcript:FBtr0305142)

HSP 1 Score: 146.362 bits (368), Expect = 1.929e-34
Identity = 110/290 (37.93%), Postives = 144/290 (49.66%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDI--NECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC--DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            +C  GY   G NC+D   NEC    C   + C NT GSF C C     Y    F C   I+EC  +  I + C  +A  C  PA+         C C  G+  D +  C D++EC N   C   A C N+ G++ C CP G+  +  +  GC D+DEC    +C    IC N+ GSYRC C  GY+     + GC+D DEC  ++  C G N  C NT GSF+C+CP G       GC D+DEC  NN C  G+ C+N  G F C C  GFV  +D    +L + LN  Q
Sbjct:  115 HCNDGYG--GCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPG--YRGNGFHCE-DIDECQ-DPAIAARCVENAECCNLPAH-------FLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDECSYPNVCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDEC--ARTPC-GRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQ 388          

HSP 2 Score: 130.568 bits (327), Expect = 1.224e-29
Identity = 100/316 (31.65%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDN-CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNP-EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE--GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK----------------------------------GCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +L   F   CKD     GY+  G+  C D++EC +   C  N+ C NTPG++ C C +     NP   GC   ++ECS+            C   A C++  G   C CP G+  D +   GC+D +EC   PC   A C N+ GSF C CP G+      GC D+DEC  +  C     C N+ GS++C C +G+    D                                    C+DIDEC     + + G N  C N PGS++C+CP G + +    C +I+EC Q+N C E + C ++ G F CTC   + 
Sbjct:  189 NLPAHFLCKCKD-----GYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQ-DVDECSYPN---------VCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINEC-QDNPCGENAICTDTVGSFVCTCKPDYT 488          

HSP 3 Score: 120.939 bits (302), Expect = 9.177e-27
Identity = 100/322 (31.06%), Postives = 136/322 (42.24%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLKEGCIDINECNM-----NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGS-YRCACHNGYEYVEDKG--CIDIDECQASKK-------------LCQ-----------------GDNVVCQNTPGSFKCVCPVGLEYKEK---GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            C  G++ IG +C+DI+EC + A  C  +++C+NTPGS+ C C        P   C                C+ V C + A C       +CVC  G+TY   D+  GC+DI+EC++       C   ATC NS G F C CP GF    H  CVD+DECR  +  C     C N+ G  Y C C        D    C+ I  C A++              LC                  G +  C    G  +C+C  G         GC DIDEC+ N  C E + C N+ GG+ C C  G   +   + C   KT+
Sbjct:  567 CLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRMACK-------------QPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRAN-PCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTV 874          

HSP 4 Score: 115.161 bits (287), Expect = 5.126e-25
Identity = 105/326 (32.21%), Postives = 140/326 (42.94%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGS-FVCYCSNNE-EYLNPEFGCAPKIEECSFERKII--SICDAVT------------CRSP---------ANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ---------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDEC--QASKKLCQGDNVVCQNTPGSFKCVCPVG--------------------LEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFN-CTCNKGFVFNNDTKFCELDK 3395
            C+D++EC + A  C   + C+N PG  + C C  N     +P   C P I  CS        SICDA              CR P         A C  +NG   C+C  G+T +  L  GC DI+EC  NPC +KA C N+ G + C CP G            S   GC D + C   + C    +  +  G+  C C  GYE   + G C D+DEC  Q  K  C G N +C+N PGS++C CP G                      YK  G  CV +  C     CP G+ CI+  GG + C C KG+    D    ++D+
Sbjct:  707 CVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVP-IVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC----VQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPAC-GLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDE 1025          

HSP 5 Score: 102.834 bits (255), Expect = 3.287e-21
Identity = 104/390 (26.67%), Postives = 146/390 (37.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSL-ACHNNSRCINTPGSFVCYCSNN---------EEYLNP----EFGCAPKIEECSFERKI----ISICDAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDK-----------------------------------------GCIDIDECQASKKLCQGDNVVCQNTPGSFK---------------------------------------CVCPVGLEYKEK----GCVDIDECQQNN----TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C  GY     N C D++EC +   C   + C+N  GSF C C +          ++   P    + G  P   + +  ++     ++  D   C  P         A C +  G   C+CP GF       C +INEC  NPC + A C ++ GSF C C   +      GCVDIDEC    K C  + +C+N +  Y C C  GY+   D                                           C+DIDEC+   ++C G +  C NTPGS+                                        CVC  G  Y       GCVDIDEC   +    +C + +TC NS GGF C C  GF  +  +K  ++D+
Sbjct:  324 CPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGYDGKPDPKVACEQVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVC-GPHAQCLNTPGSYGCECEAGYVGSPPRMACKQPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDE 712          

HSP 6 Score: 84.3445 bits (207), Expect = 1.201e-15
Identity = 75/287 (26.13%), Postives = 111/287 (38.68%), Query Frame = 3
Query: 2577 CGKGY---QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANC--SSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN----PCLDKATCRNSYGSFDCFCPKGFQYS---------------------VHFGCVDIDECRDSKICSGNGICQNMIGSYR-CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---------YKEKGCVDIDECQQNNTCPEGSTCI 3314
            C  GY     +   C DI+EC +  C   + C NT G ++C C           GC           K +   DA  C +   C   S  G   C+C +G+  + + G C D++EC++      C   A C+N  GS++C CP+G   +                     V   CV +  C   + C     C ++ G    CAC  GY+   D  C+D+DEC+            C N PGS+ C CP G +           ++ C    EC  N  C +   C+
Sbjct:  813 CAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGC--------ITSKTVGCSDANPCATGETCVQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECV 1090          

HSP 7 Score: 72.0182 bits (175), Expect = 6.586e-12
Identity = 83/309 (26.86%), Postives = 119/309 (38.51%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIIS----------IC---------DAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECR--------------DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCIN 3317
            C KGYQ   D +C+D++EC+      C   ++C+N PGS+ C+C   E Y    +   CA    +C+ +R+  +          +C         D   C+SP         A C+ S+  + C+C  GF  D   GC D +EC+  PC   A C N  G + C CPK +              +    C+  D+C                S +C  N  C+    +  C C  GY    D  C+     Q    +C GD  +C  T     C CP G        G    D+C     C E   CIN
Sbjct: 1008 CPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCP--EGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQ-CMCEAGFKGDPLLGCTDEDECSHLPCAYGAYCVNKKGGYQCVCPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGDCVS----QCQDVIC-GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICIN 1308          

HSP 8 Score: 60.8474 bits (146), Expect = 1.558e-8
Identity = 78/284 (27.46%), Postives = 111/284 (39.08%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD-IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNN-------DTKFCE 3386
            C  GY K GD +C+  ++C  + C + + CI T     C C   +   NP  G +   ++CS  R                       E  +C  G   +  EG +         C   ATC  + G   C C   F  +    C+  I++ + S  C  N  C+  +G  RCAC+ G      +GC        SK +CQ    G N  C+       C+CP G       GC D+DEC  N  C   + C+N  GGF C C  G   N        ++KFC+
Sbjct: 1237 CRVGYVKNGDGDCV--SQCQDVICGDGALCIPTSEGPTCKCPQGQ-LGNPFPGGSCSTDQCSAARPC--------------------GERQICINGRCKERCEGVV---------CGIGATCDRNNGK--CICEPNFVGNPDLICMPPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCG-----AQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDEC-ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQ 1480          

HSP 9 Score: 60.4622 bits (145), Expect = 2.310e-8
Identity = 82/290 (28.28%), Postives = 112/290 (38.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFER--KIISICDAVTCRSP----------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN------------PCLDKATCRNSYGSFD----------CFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCI-NSFGGFNC 3338
            C +GYQ + D C DINEC S  CH+ + C N PGS+ C C         + GC     EC  +      + C    CRSP          ANC +      C CP     D    C+ I EC  N             C+D  +  N+ G+            C C  G       GCV +  C+    C+   IC + I S  C+         ++ CI       S++LC QG   VCQ T  S    CP         C    EC+ ++ C E  TC+ +++G   C
Sbjct: 2380 CYEGYQLVNDVCEDINECLSQPCHSTAFCNNLPGSYSCQCPEGLIGDPLQAGCR-DPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHI-ECADNDDCSGEKACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCS--------TNRDCI-------SEQLCLQG---VCQGTCKSNS-SCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKC 2648          

HSP 10 Score: 56.225 bits (134), Expect = 4.104e-7
Identity = 56/204 (27.45%), Postives = 81/204 (39.71%), Query Frame = 3
Query: 2634 SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVG 3233
            S  C  N+ C    G   C C N   + NP  GC  + +         ++C   +C   A C +      C+CP+GF+ +   GC D++EC   PC   A C N  G F+C C  G   + +  C  I    +SK C     CQ    + R  C  GY   + +           K LC     G   +C     +  C+CP+G
Sbjct: 1357 SPGCGENAHCEYGLGQSRCAC-NPGTFGNPYEGCGAQSK---------NVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDECANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPI----ESKFCQDANKCQ---CNERVECPEGYSCQKGQ----------CKNLCSQASCGPRAICD----AGNCICPMG 1529          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Match: dpy (gene:FBgn0053196 transcript:FBtr0305141)

HSP 1 Score: 145.976 bits (367), Expect = 2.266e-34
Identity = 110/290 (37.93%), Postives = 144/290 (49.66%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDI--NECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC--DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            +C  GY   G NC+D   NEC    C   + C NT GSF C C     Y    F C   I+EC  +  I + C  +A  C  PA+         C C  G+  D +  C D++EC N   C   A C N+ G++ C CP G+  +  +  GC D+DEC    +C    IC N+ GSYRC C  GY+     + GC+D DEC  ++  C G N  C NT GSF+C+CP G       GC D+DEC  NN C  G+ C+N  G F C C  GFV  +D    +L + LN  Q
Sbjct:  115 HCNDGYG--GCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPG--YRGNGFHCE-DIDECQ-DPAIAARCVENAECCNLPAH-------FLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDECSYPNVCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDEC--ARTPC-GRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQ 388          

HSP 2 Score: 130.183 bits (326), Expect = 1.438e-29
Identity = 100/316 (31.65%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDN-CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNP-EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE--GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK----------------------------------GCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +L   F   CKD     GY+  G+  C D++EC +   C  N+ C NTPG++ C C +     NP   GC   ++ECS+            C   A C++  G   C CP G+  D +   GC+D +EC   PC   A C N+ GSF C CP G+      GC D+DEC  +  C     C N+ GS++C C +G+    D                                    C+DIDEC     + + G N  C N PGS++C+CP G + +    C +I+EC Q+N C E + C ++ G F CTC   + 
Sbjct:  189 NLPAHFLCKCKD-----GYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQ-DVDECSYPN---------VCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINEC-QDNPCGENAICTDTVGSFVCTCKPDYT 488          

HSP 3 Score: 120.939 bits (302), Expect = 1.078e-26
Identity = 100/322 (31.06%), Postives = 136/322 (42.24%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLKEGCIDINECNM-----NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGS-YRCACHNGYEYVEDKG--CIDIDECQASKK-------------LCQ-----------------GDNVVCQNTPGSFKCVCPVGLEYKEK---GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            C  G++ IG +C+DI+EC + A  C  +++C+NTPGS+ C C        P   C                C+ V C + A C       +CVC  G+TY   D+  GC+DI+EC++       C   ATC NS G F C CP GF    H  CVD+DECR  +  C     C N+ G  Y C C        D    C+ I  C A++              LC                  G +  C    G  +C+C  G         GC DIDEC+ N  C E + C N+ GG+ C C  G   +   + C   KT+
Sbjct:  567 CLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRMACK-------------QPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRAN-PCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTV 874          

HSP 4 Score: 115.161 bits (287), Expect = 5.870e-25
Identity = 105/326 (32.21%), Postives = 140/326 (42.94%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGS-FVCYCSNNE-EYLNPEFGCAPKIEECSFERKII--SICDAVT------------CRSP---------ANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ---------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDEC--QASKKLCQGDNVVCQNTPGSFKCVCPVG--------------------LEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFN-CTCNKGFVFNNDTKFCELDK 3395
            C+D++EC + A  C   + C+N PG  + C C  N     +P   C P I  CS        SICDA              CR P         A C  +NG   C+C  G+T +  L  GC DI+EC  NPC +KA C N+ G + C CP G            S   GC D + C   + C    +  +  G+  C C  GYE   + G C D+DEC  Q  K  C G N +C+N PGS++C CP G                      YK  G  CV +  C     CP G+ CI+  GG + C C KG+    D    ++D+
Sbjct:  707 CVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVP-IVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC----VQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPAC-GLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDE 1025          

HSP 5 Score: 102.449 bits (254), Expect = 4.025e-21
Identity = 104/390 (26.67%), Postives = 146/390 (37.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSL-ACHNNSRCINTPGSFVCYCSNN---------EEYLNP----EFGCAPKIEECSFERKI----ISICDAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-----------------------------------------KGCIDIDECQASKKLCQGDNVVCQNTPGSFK---------------------------------------CVCPVGLEYKEK----GCVDIDECQQNN----TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C  GY     N C D++EC +   C   + C+N  GSF C C +          ++   P    + G  P   + +  ++     ++  D   C  P         A C +  G   C+CP GF       C +INEC  NPC + A C ++ GSF C C   +      GCVDIDEC    K C  + +C+N +  Y C C  GY+   D                                           C+DIDEC+   ++C G +  C NTPGS+                                        CVC  G  Y       GCVDIDEC   +    +C + +TC NS GGF C C  GF  +  +K  ++D+
Sbjct:  324 CPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGYDGKPDPKVACEQVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVC-GPHAQCLNTPGSYGCECEAGYVGSPPRMACKQPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDE 712          

HSP 6 Score: 83.9593 bits (206), Expect = 1.495e-15
Identity = 75/287 (26.13%), Postives = 111/287 (38.68%), Query Frame = 3
Query: 2577 CGKGY---QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANC--SSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN----PCLDKATCRNSYGSFDCFCPKGFQYS---------------------VHFGCVDIDECRDSKICSGNGICQNMIGSYR-CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---------YKEKGCVDIDECQQNNTCPEGSTCI 3314
            C  GY     +   C DI+EC +  C   + C NT G ++C C           GC           K +   DA  C +   C   S  G   C+C +G+  + + G C D++EC++      C   A C+N  GS++C CP+G   +                     V   CV +  C   + C     C ++ G    CAC  GY+   D  C+D+DEC+            C N PGS+ C CP G +           ++ C    EC  N  C +   C+
Sbjct:  813 CAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGC--------ITSKTVGCSDANPCATGETCVQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECV 1090          

HSP 7 Score: 71.633 bits (174), Expect = 7.665e-12
Identity = 83/309 (26.86%), Postives = 119/309 (38.51%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIIS----------IC---------DAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECR--------------DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCIN 3317
            C KGYQ   D +C+D++EC+      C   ++C+N PGS+ C+C   E Y    +   CA    +C+ +R+  +          +C         D   C+SP         A C+ S+  + C+C  GF  D   GC D +EC+  PC   A C N  G + C CPK +              +    C+  D+C                S +C  N  C+    +  C C  GY    D  C+     Q    +C GD  +C  T     C CP G        G    D+C     C E   CIN
Sbjct: 1008 CPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCP--EGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQ-CMCEAGFKGDPLLGCTDEDECSHLPCAYGAYCVNKKGGYQCVCPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGDCVS----QCQDVIC-GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICIN 1308          

HSP 8 Score: 60.4622 bits (145), Expect = 1.859e-8
Identity = 78/284 (27.46%), Postives = 111/284 (39.08%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD-IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNN-------DTKFCE 3386
            C  GY K GD +C+  ++C  + C + + CI T     C C   +   NP  G +   ++CS  R                       E  +C  G   +  EG +         C   ATC  + G   C C   F  +    C+  I++ + S  C  N  C+  +G  RCAC+ G      +GC        SK +CQ    G N  C+       C+CP G       GC D+DEC  N  C   + C+N  GGF C C  G   N        ++KFC+
Sbjct: 1237 CRVGYVKNGDGDCV--SQCQDVICGDGALCIPTSEGPTCKCPQGQ-LGNPFPGGSCSTDQCSAARPC--------------------GERQICINGRCKERCEGVV---------CGIGATCDRNNGK--CICEPNFVGNPDLICMPPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCG-----AQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDEC-ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQ 1480          

HSP 9 Score: 60.077 bits (144), Expect = 2.780e-8
Identity = 82/290 (28.28%), Postives = 112/290 (38.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFER--KIISICDAVTCRSP----------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN------------PCLDKATCRNSYGSFD----------CFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCI-NSFGGFNC 3338
            C +GYQ + D C DINEC S  CH+ + C N PGS+ C C         + GC     EC  +      + C    CRSP          ANC +      C CP     D    C+ I EC  N             C+D  +  N+ G+            C C  G       GCV +  C+    C+   IC + I S  C+         ++ CI       S++LC QG   VCQ T  S    CP         C    EC+ ++ C E  TC+ +++G   C
Sbjct: 2380 CYEGYQLVNDVCEDINECLSQPCHSTAFCNNLPGSYSCQCPEGLIGDPLQAGCR-DPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHI-ECADNDDCSGEKACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCS--------TNRDCI-------SEQLCLQG---VCQGTCKSNS-SCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKC 2648          

HSP 10 Score: 55.8398 bits (133), Expect = 4.735e-7
Identity = 56/204 (27.45%), Postives = 81/204 (39.71%), Query Frame = 3
Query: 2634 SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVG 3233
            S  C  N+ C    G   C C N   + NP  GC  + +         ++C   +C   A C +      C+CP+GF+ +   GC D++EC   PC   A C N  G F+C C  G   + +  C  I    +SK C     CQ    + R  C  GY   + +           K LC     G   +C     +  C+CP+G
Sbjct: 1357 SPGCGENAHCEYGLGQSRCAC-NPGTFGNPYEGCGAQSK---------NVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDECANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPI----ESKFCQDANKCQ---CNERVECPEGYSCQKGQ----------CKNLCSQASCGPRAICD----AGNCICPMG 1529          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Match: dpy (gene:FBgn0053196 transcript:FBtr0332352)

HSP 1 Score: 145.976 bits (367), Expect = 2.608e-34
Identity = 110/290 (37.93%), Postives = 144/290 (49.66%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDI--NECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC--DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            +C  GY   G NC+D   NEC    C   + C NT GSF C C     Y    F C   I+EC  +  I + C  +A  C  PA+         C C  G+  D +  C D++EC N   C   A C N+ G++ C CP G+  +  +  GC D+DEC    +C    IC N+ GSYRC C  GY+     + GC+D DEC  ++  C G N  C NT GSF+C+CP G       GC D+DEC  NN C  G+ C+N  G F C C  GFV  +D    +L + LN  Q
Sbjct:  115 HCNDGYG--GCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPG--YRGNGFHCE-DIDECQ-DPAIAARCVENAECCNLPAH-------FLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDECSYPNVCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDEC--ARTPC-GRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQ 388          

HSP 2 Score: 130.183 bits (326), Expect = 1.771e-29
Identity = 100/316 (31.65%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDN-CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNP-EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE--GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK----------------------------------GCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +L   F   CKD     GY+  G+  C D++EC +   C  N+ C NTPG++ C C +     NP   GC   ++ECS+            C   A C++  G   C CP G+  D +   GC+D +EC   PC   A C N+ GSF C CP G+      GC D+DEC  +  C     C N+ GS++C C +G+    D                                    C+DIDEC     + + G N  C N PGS++C+CP G + +    C +I+EC Q+N C E + C ++ G F CTC   + 
Sbjct:  189 NLPAHFLCKCKD-----GYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQ-DVDECSYPN---------VCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINEC-QDNPCGENAICTDTVGSFVCTCKPDYT 488          

HSP 3 Score: 120.553 bits (301), Expect = 1.350e-26
Identity = 100/322 (31.06%), Postives = 136/322 (42.24%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLKEGCIDINECNM-----NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGS-YRCACHNGYEYVEDKG--CIDIDECQASKK-------------LCQ-----------------GDNVVCQNTPGSFKCVCPVGLEYKEK---GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            C  G++ IG +C+DI+EC + A  C  +++C+NTPGS+ C C        P   C                C+ V C + A C       +CVC  G+TY   D+  GC+DI+EC++       C   ATC NS G F C CP GF    H  CVD+DECR  +  C     C N+ G  Y C C        D    C+ I  C A++              LC                  G +  C    G  +C+C  G         GC DIDEC+ N  C E + C N+ GG+ C C  G   +   + C   KT+
Sbjct:  567 CLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRMACK-------------QPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRAN-PCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTV 874          

HSP 4 Score: 114.775 bits (286), Expect = 7.796e-25
Identity = 105/326 (32.21%), Postives = 140/326 (42.94%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGS-FVCYCSNNE-EYLNPEFGCAPKIEECSFERKII--SICDAVT------------CRSP---------ANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ---------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDEC--QASKKLCQGDNVVCQNTPGSFKCVCPVG--------------------LEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFN-CTCNKGFVFNNDTKFCELDK 3395
            C+D++EC + A  C   + C+N PG  + C C  N     +P   C P I  CS        SICDA              CR P         A C  +NG   C+C  G+T +  L  GC DI+EC  NPC +KA C N+ G + C CP G            S   GC D + C   + C    +  +  G+  C C  GYE   + G C D+DEC  Q  K  C G N +C+N PGS++C CP G                      YK  G  CV +  C     CP G+ CI+  GG + C C KG+    D    ++D+
Sbjct:  707 CVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVP-IVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC----VQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPAC-GLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDE 1025          

HSP 5 Score: 102.064 bits (253), Expect = 4.875e-21
Identity = 104/390 (26.67%), Postives = 146/390 (37.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSL-ACHNNSRCINTPGSFVCYCSNN---------EEYLNP----EFGCAPKIEECSFERKI----ISICDAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-----------------------------------------KGCIDIDECQASKKLCQGDNVVCQNTPGSFK---------------------------------------CVCPVGLEYKEK----GCVDIDECQQNN----TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C  GY     N C D++EC +   C   + C+N  GSF C C +          ++   P    + G  P   + +  ++     ++  D   C  P         A C +  G   C+CP GF       C +INEC  NPC + A C ++ GSF C C   +      GCVDIDEC    K C  + +C+N +  Y C C  GY+   D                                           C+DIDEC+   ++C G +  C NTPGS+                                        CVC  G  Y       GCVDIDEC   +    +C + +TC NS GGF C C  GF  +  +K  ++D+
Sbjct:  324 CPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGYDGKPDPKVACEQVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVC-GPHAQCLNTPGSYGCECEAGYVGSPPRMACKQPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDE 712          

HSP 6 Score: 83.9593 bits (206), Expect = 1.752e-15
Identity = 75/287 (26.13%), Postives = 111/287 (38.68%), Query Frame = 3
Query: 2577 CGKGY---QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANC--SSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN----PCLDKATCRNSYGSFDCFCPKGFQYS---------------------VHFGCVDIDECRDSKICSGNGICQNMIGSYR-CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---------YKEKGCVDIDECQQNNTCPEGSTCI 3314
            C  GY     +   C DI+EC +  C   + C NT G ++C C           GC           K +   DA  C +   C   S  G   C+C +G+  + + G C D++EC++      C   A C+N  GS++C CP+G   +                     V   CV +  C   + C     C ++ G    CAC  GY+   D  C+D+DEC+            C N PGS+ C CP G +           ++ C    EC  N  C +   C+
Sbjct:  813 CAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGC--------ITSKTVGCSDANPCATGETCVQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECV 1090          

HSP 7 Score: 71.633 bits (174), Expect = 9.286e-12
Identity = 83/309 (26.86%), Postives = 119/309 (38.51%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIIS----------IC---------DAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECR--------------DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCIN 3317
            C KGYQ   D +C+D++EC+      C   ++C+N PGS+ C+C   E Y    +   CA    +C+ +R+  +          +C         D   C+SP         A C+ S+  + C+C  GF  D   GC D +EC+  PC   A C N  G + C CPK +              +    C+  D+C                S +C  N  C+    +  C C  GY    D  C+     Q    +C GD  +C  T     C CP G        G    D+C     C E   CIN
Sbjct: 1008 CPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCP--EGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQ-CMCEAGFKGDPLLGCTDEDECSHLPCAYGAYCVNKKGGYQCVCPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGDCVS----QCQDVIC-GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICIN 1308          

HSP 8 Score: 60.4622 bits (145), Expect = 2.071e-8
Identity = 78/284 (27.46%), Postives = 111/284 (39.08%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD-IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNN-------DTKFCE 3386
            C  GY K GD +C+  ++C  + C + + CI T     C C   +   NP  G +   ++CS  R                       E  +C  G   +  EG +         C   ATC  + G   C C   F  +    C+  I++ + S  C  N  C+  +G  RCAC+ G      +GC        SK +CQ    G N  C+       C+CP G       GC D+DEC  N  C   + C+N  GGF C C  G   N        ++KFC+
Sbjct: 1237 CRVGYVKNGDGDCV--SQCQDVICGDGALCIPTSEGPTCKCPQGQ-LGNPFPGGSCSTDQCSAARPC--------------------GERQICINGRCKERCEGVV---------CGIGATCDRNNGK--CICEPNFVGNPDLICMPPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCG-----AQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDEC-ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQ 1480          

HSP 9 Score: 59.6918 bits (143), Expect = 3.124e-8
Identity = 82/290 (28.28%), Postives = 112/290 (38.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFER--KIISICDAVTCRSP----------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN------------PCLDKATCRNSYGSFD----------CFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCI-NSFGGFNC 3338
            C +GYQ + D C DINEC S  CH+ + C N PGS+ C C         + GC     EC  +      + C    CRSP          ANC +      C CP     D    C+ I EC  N             C+D  +  N+ G+            C C  G       GCV +  C+    C+   IC + I S  C+         ++ CI       S++LC QG   VCQ T  S    CP         C    EC+ ++ C E  TC+ +++G   C
Sbjct: 2380 CYEGYQLVNDVCEDINECLSQPCHSTAFCNNLPGSYSCQCPEGLIGDPLQAGCR-DPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHI-ECADNDDCSGEKACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCS--------TNRDCI-------SEQLCLQG---VCQGTCKSNS-SCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKC 2648          

HSP 10 Score: 55.8398 bits (133), Expect = 5.277e-7
Identity = 56/204 (27.45%), Postives = 81/204 (39.71%), Query Frame = 3
Query: 2634 SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVG 3233
            S  C  N+ C    G   C C N   + NP  GC  + +         ++C   +C   A C +      C+CP+GF+ +   GC D++EC   PC   A C N  G F+C C  G   + +  C  I    +SK C     CQ    + R  C  GY   + +           K LC     G   +C     +  C+CP+G
Sbjct: 1357 SPGCGENAHCEYGLGQSRCAC-NPGTFGNPYEGCGAQSK---------NVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDECANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPI----ESKFCQDANKCQ---CNERVECPEGYSCQKGQ----------CKNLCSQASCGPRAICD----AGNCICPMG 1529          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Match: dpy (gene:FBgn0053196 transcript:FBtr0332344)

HSP 1 Score: 144.821 bits (364), Expect = 5.263e-34
Identity = 110/290 (37.93%), Postives = 144/290 (49.66%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDI--NECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC--DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            +C  GY   G NC+D   NEC    C   + C NT GSF C C     Y    F C   I+EC  +  I + C  +A  C  PA+         C C  G+  D +  C D++EC N   C   A C N+ G++ C CP G+  +  +  GC D+DEC    +C    IC N+ GSYRC C  GY+     + GC+D DEC  ++  C G N  C NT GSF+C+CP G       GC D+DEC  NN C  G+ C+N  G F C C  GFV  +D    +L + LN  Q
Sbjct:  115 HCNDGYG--GCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPG--YRGNGFHCE-DIDECQ-DPAIAARCVENAECCNLPAH-------FLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDECSYPNVCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDEC--ARTPC-GRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQ 388          

HSP 2 Score: 129.028 bits (323), Expect = 3.307e-29
Identity = 100/316 (31.65%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDN-CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNP-EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE--GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK----------------------------------GCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +L   F   CKD     GY+  G+  C D++EC +   C  N+ C NTPG++ C C +     NP   GC   ++ECS+            C   A C++  G   C CP G+  D +   GC+D +EC   PC   A C N+ GSF C CP G+      GC D+DEC  +  C     C N+ GS++C C +G+    D                                    C+DIDEC     + + G N  C N PGS++C+CP G + +    C +I+EC Q+N C E + C ++ G F CTC   + 
Sbjct:  189 NLPAHFLCKCKD-----GYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQ-DVDECSYPN---------VCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINEC-QDNPCGENAICTDTVGSFVCTCKPDYT 488          

HSP 3 Score: 119.783 bits (299), Expect = 2.476e-26
Identity = 100/322 (31.06%), Postives = 136/322 (42.24%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLKEGCIDINECNM-----NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGS-YRCACHNGYEYVEDKG--CIDIDECQASKK-------------LCQ-----------------GDNVVCQNTPGSFKCVCPVGLEYKEK---GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            C  G++ IG +C+DI+EC + A  C  +++C+NTPGS+ C C        P   C                C+ V C + A C       +CVC  G+TY   D+  GC+DI+EC++       C   ATC NS G F C CP GF    H  CVD+DECR  +  C     C N+ G  Y C C        D    C+ I  C A++              LC                  G +  C    G  +C+C  G         GC DIDEC+ N  C E + C N+ GG+ C C  G   +   + C   KT+
Sbjct:  567 CLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRMACK-------------QPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRAN-PCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTV 874          

HSP 4 Score: 113.62 bits (283), Expect = 1.719e-24
Identity = 105/326 (32.21%), Postives = 140/326 (42.94%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGS-FVCYCSNNE-EYLNPEFGCAPKIEECSFERKII--SICDAVT------------CRSP---------ANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ---------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDEC--QASKKLCQGDNVVCQNTPGSFKCVCPVG--------------------LEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFN-CTCNKGFVFNNDTKFCELDK 3395
            C+D++EC + A  C   + C+N PG  + C C  N     +P   C P I  CS        SICDA              CR P         A C  +NG   C+C  G+T +  L  GC DI+EC  NPC +KA C N+ G + C CP G            S   GC D + C   + C    +  +  G+  C C  GYE   + G C D+DEC  Q  K  C G N +C+N PGS++C CP G                      YK  G  CV +  C     CP G+ CI+  GG + C C KG+    D    ++D+
Sbjct:  707 CVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVP-IVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC----VQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPAC-GLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDE 1025          

HSP 5 Score: 101.293 bits (251), Expect = 8.850e-21
Identity = 104/390 (26.67%), Postives = 146/390 (37.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSL-ACHNNSRCINTPGSFVCYCSNN---------EEYLNP----EFGCAPKIEECSFERKI----ISICDAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDK-----------------------------------------GCIDIDECQASKKLCQGDNVVCQNTPGSFK---------------------------------------CVCPVGLEYKEK----GCVDIDECQQNN----TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C  GY     N C D++EC +   C   + C+N  GSF C C +          ++   P    + G  P   + +  ++     ++  D   C  P         A C +  G   C+CP GF       C +INEC  NPC + A C ++ GSF C C   +      GCVDIDEC    K C  + +C+N +  Y C C  GY+   D                                           C+DIDEC+   ++C G +  C NTPGS+                                        CVC  G  Y       GCVDIDEC   +    +C + +TC NS GGF C C  GF  +  +K  ++D+
Sbjct:  324 CPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGYDGKPDPKVACEQVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVC-GPHAQCLNTPGSYGCECEAGYVGSPPRMACKQPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDE 712          

HSP 6 Score: 82.8037 bits (203), Expect = 4.014e-15
Identity = 75/287 (26.13%), Postives = 111/287 (38.68%), Query Frame = 3
Query: 2577 CGKGY---QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANC--SSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN----PCLDKATCRNSYGSFDCFCPKGFQYS---------------------VHFGCVDIDECRDSKICSGNGICQNMIGSYR-CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---------YKEKGCVDIDECQQNNTCPEGSTCI 3314
            C  GY     +   C DI+EC +  C   + C NT G ++C C           GC           K +   DA  C +   C   S  G   C+C +G+  + + G C D++EC++      C   A C+N  GS++C CP+G   +                     V   CV +  C   + C     C ++ G    CAC  GY+   D  C+D+DEC+            C N PGS+ C CP G +           ++ C    EC  N  C +   C+
Sbjct:  813 CAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGC--------ITSKTVGCSDANPCATGETCVQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECV 1090          

HSP 7 Score: 70.4774 bits (171), Expect = 1.890e-11
Identity = 83/309 (26.86%), Postives = 119/309 (38.51%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIIS----------IC---------DAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG------------CVDIDECR--------------DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCIN 3317
            C KGYQ   D +C+D++EC+      C   ++C+N PGS+ C+C   E Y    +   CA    +C+ +R+  +          +C         D   C+SP         A C+ S+  + C+C  GF  D   GC D +EC+  PC   A C N  G + C CPK +    +              C+  D+C                S +C  N  C+    +  C C  GY    D  C+     Q    +C GD  +C  T     C CP G        G    D+C     C E   CIN
Sbjct: 1008 CPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCP--EGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQ-CMCEAGFKGDPLLGCTDEDECSHLPCAYGAYCVNKKGGYQCVCPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGDCVS----QCQDVIC-GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICIN 1308          

HSP 8 Score: 59.6918 bits (143), Expect = 3.871e-8
Identity = 78/284 (27.46%), Postives = 111/284 (39.08%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD-IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNN-------DTKFCE 3386
            C  GY K GD +C+  ++C  + C + + CI T     C C   +   NP  G +   ++CS  R                       E  +C  G   +  EG +         C   ATC  + G   C C   F  +    C+  I++ + S  C  N  C+  +G  RCAC+ G      +GC        SK +CQ    G N  C+       C+CP G       GC D+DEC  N  C   + C+N  GGF C C  G   N        ++KFC+
Sbjct: 1237 CRVGYVKNGDGDCV--SQCQDVICGDGALCIPTSEGPTCKCPQGQ-LGNPFPGGSCSTDQCSAARPC--------------------GERQICINGRCKERCEGVV---------CGIGATCDRNNG--KCICEPNFVGNPDLICMPPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCG-----AQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDEC-ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQ 1480          

HSP 9 Score: 58.9214 bits (141), Expect = 5.741e-8
Identity = 82/290 (28.28%), Postives = 112/290 (38.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFER--KIISICDAVTCRSP----------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN------------PCLDKATCRNSYGSFD----------CFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCI-NSFGGFNC 3338
            C +GYQ + D C DINEC S  CH+ + C N PGS+ C C         + GC     EC  +      + C    CRSP          ANC +      C CP     D    C+ I EC  N             C+D  +  N+ G+            C C  G       GCV +  C+    C+   IC + I S  C+         ++ CI       S++LC QG   VCQ T  S    CP         C    EC+ ++ C E  TC+ +++G   C
Sbjct: 2380 CYEGYQLVNDVCEDINECLSQPCHSTAFCNNLPGSYSCQCPEGLIGDPLQAGCR-DPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHI-ECADNDDCSGEKACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCS--------TNRDCI-------SEQLCLQG---VCQGTCKSNS-SCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKC 2648          

HSP 10 Score: 55.0694 bits (131), Expect = 9.857e-7
Identity = 56/204 (27.45%), Postives = 81/204 (39.71%), Query Frame = 3
Query: 2634 SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVG 3233
            S  C  N+ C    G   C C N   + NP  GC  + +         ++C   +C   A C +      C+CP+GF+ +   GC D++EC   PC   A C N  G F+C C  G   + +  C  I    +SK C     CQ    + R  C  GY   + +           K LC     G   +C     +  C+CP+G
Sbjct: 1357 SPGCGENAHCEYGLGQSRCAC-NPGTFGNPYEGCGAQSK---------NVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDECANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPI----ESKFCQDANKCQ---CNERVECPEGYSCQKGQ----------CKNLCSQASCGPRAICD----AGNCICPMG 1529          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Match: dpy (gene:FBgn0053196 transcript:FBtr0332349)

HSP 1 Score: 144.821 bits (364), Expect = 6.448e-34
Identity = 110/290 (37.93%), Postives = 144/290 (49.66%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDI--NECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC--DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            +C  GY   G NC+D   NEC    C   + C NT GSF C C     Y    F C   I+EC  +  I + C  +A  C  PA+         C C  G+  D +  C D++EC N   C   A C N+ G++ C CP G+  +  +  GC D+DEC    +C    IC N+ GSYRC C  GY+     + GC+D DEC  ++  C G N  C NT GSF+C+CP G       GC D+DEC  NN C  G+ C+N  G F C C  GFV  +D    +L + LN  Q
Sbjct:  115 HCNDGYG--GCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPG--YRGNGFHCE-DIDECQ-DPAIAARCVENAECCNLPAH-------FLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDECSYPNVCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDEC--ARTPC-GRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQ 388          

HSP 2 Score: 128.642 bits (322), Expect = 4.296e-29
Identity = 100/316 (31.65%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDN-CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNP-EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE--GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK----------------------------------GCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +L   F   CKD     GY+  G+  C D++EC +   C  N+ C NTPG++ C C +     NP   GC   ++ECS+            C   A C++  G   C CP G+  D +   GC+D +EC   PC   A C N+ GSF C CP G+      GC D+DEC  +  C     C N+ GS++C C +G+    D                                    C+DIDEC     + + G N  C N PGS++C+CP G + +    C +I+EC Q+N C E + C ++ G F CTC   + 
Sbjct:  189 NLPAHFLCKCKD-----GYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQ-DVDECSYPN---------VCGPGAICTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINEC-QDNPCGENAICTDTVGSFVCTCKPDYT 488          

HSP 3 Score: 119.398 bits (298), Expect = 3.298e-26
Identity = 100/322 (31.06%), Postives = 136/322 (42.24%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLKEGCIDINECNM-----NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGS-YRCACHNGYEYVEDKG--CIDIDECQASKK-------------LCQ-----------------GDNVVCQNTPGSFKCVCPVGLEYKEK---GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            C  G++ IG +C+DI+EC + A  C  +++C+NTPGS+ C C        P   C                C+ V C + A C       +CVC  G+TY   D+  GC+DI+EC++       C   ATC NS G F C CP GF    H  CVD+DECR  +  C     C N+ G  Y C C        D    C+ I  C A++              LC                  G +  C    G  +C+C  G         GC DIDEC+ N  C E + C N+ GG+ C C  G   +   + C   KT+
Sbjct:  567 CLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRMACK-------------QPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRAN-PCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTV 874          

HSP 4 Score: 113.235 bits (282), Expect = 2.252e-24
Identity = 105/326 (32.21%), Postives = 140/326 (42.94%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGS-FVCYCSNNE-EYLNPEFGCAPKIEECSFERKII--SICDAVT------------CRSP---------ANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ---------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDEC--QASKKLCQGDNVVCQNTPGSFKCVCPVG--------------------LEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFN-CTCNKGFVFNNDTKFCELDK 3395
            C+D++EC + A  C   + C+N PG  + C C  N     +P   C P I  CS        SICDA              CR P         A C  +NG   C+C  G+T +  L  GC DI+EC  NPC +KA C N+ G + C CP G            S   GC D + C   + C    +  +  G+  C C  GYE   + G C D+DEC  Q  K  C G N +C+N PGS++C CP G                      YK  G  CV +  C     CP G+ CI+  GG + C C KG+    D    ++D+
Sbjct:  707 CVDVDECRTGASKCGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVP-IVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGAHAQCMLANGQAQCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC----VQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPAC-GLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDE 1025          

HSP 5 Score: 100.908 bits (250), Expect = 1.084e-20
Identity = 104/390 (26.67%), Postives = 146/390 (37.44%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSL-ACHNNSRCINTPGSFVCYCSNN---------EEYLNP----EFGCAPKIEECSFERKI----ISICDAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDK-----------------------------------------GCIDIDECQASKKLCQGDNVVCQNTPGSFK---------------------------------------CVCPVGLEYKEK----GCVDIDECQQNN----TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C  GY     N C D++EC +   C   + C+N  GSF C C +          ++   P    + G  P   + +  ++     ++  D   C  P         A C +  G   C+CP GF       C +INEC  NPC + A C ++ GSF C C   +      GCVDIDEC    K C  + +C+N +  Y C C  GY+   D                                           C+DIDEC+   ++C G +  C NTPGS+                                        CVC  G  Y       GCVDIDEC   +    +C + +TC NS GGF C C  GF  +  +K  ++D+
Sbjct:  324 CPDGYSGDPMNGCEDVDECATNNPCGLGAECVNLGGSFQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSYRCLCPSGFQGQGYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGYDGKPDPKVACEQVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVC-GPHAQCLNTPGSYGCECEAGYVGSPPRMACKQPCEDVRCGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCVDVDE 712          

HSP 6 Score: 82.4185 bits (202), Expect = 5.081e-15
Identity = 75/287 (26.13%), Postives = 111/287 (38.68%), Query Frame = 3
Query: 2577 CGKGY---QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANC--SSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN----PCLDKATCRNSYGSFDCFCPKGFQYS---------------------VHFGCVDIDECRDSKICSGNGICQNMIGSYR-CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---------YKEKGCVDIDECQQNNTCPEGSTCI 3314
            C  GY     +   C DI+EC +  C   + C NT G ++C C           GC           K +   DA  C +   C   S  G   C+C +G+  + + G C D++EC++      C   A C+N  GS++C CP+G   +                     V   CV +  C   + C     C ++ G    CAC  GY+   D  C+D+DEC+            C N PGS+ C CP G +           ++ C    EC  N  C +   C+
Sbjct:  813 CAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGC--------ITSKTVGCSDANPCATGETCVQDSYTGNSVCICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNSCV-LSGCSSGQACPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECV 1090          

HSP 7 Score: 70.0922 bits (170), Expect = 2.392e-11
Identity = 83/309 (26.86%), Postives = 119/309 (38.51%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIIS----------IC---------DAVTCRSP---------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG------------CVDIDECR--------------DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCIN 3317
            C KGYQ   D +C+D++EC+      C   ++C+N PGS+ C+C   E Y    +   CA    +C+ +R+  +          +C         D   C+SP         A C+ S+  + C+C  GF  D   GC D +EC+  PC   A C N  G + C CPK +    +              C+  D+C                S +C  N  C+    +  C C  GY    D  C+     Q    +C GD  +C  T     C CP G        G    D+C     C E   CIN
Sbjct: 1008 CPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCP--EGYQGDAYNGLCALAQRKCAADRECAANEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQ-CMCEAGFKGDPLLGCTDEDECSHLPCAYGAYCVNKKGGYQCVCPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGDCVS----QCQDVIC-GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICIN 1308          

HSP 8 Score: 59.3066 bits (142), Expect = 4.736e-8
Identity = 78/284 (27.46%), Postives = 111/284 (39.08%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD-IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNN-------DTKFCE 3386
            C  GY K GD +C+  ++C  + C + + CI T     C C   +   NP  G +   ++CS  R                       E  +C  G   +  EG +         C   ATC  + G   C C   F  +    C+  I++ + S  C  N  C+  +G  RCAC+ G      +GC        SK +CQ    G N  C+       C+CP G       GC D+DEC  N  C   + C+N  GGF C C  G   N        ++KFC+
Sbjct: 1237 CRVGYVKNGDGDCV--SQCQDVICGDGALCIPTSEGPTCKCPQGQ-LGNPFPGGSCSTDQCSAARPC--------------------GERQICINGRCKERCEGVV---------CGIGATCDRNNG--KCICEPNFVGNPDLICMPPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCG-----AQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDEC-ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQ 1480          

HSP 9 Score: 58.9214 bits (141), Expect = 6.793e-8
Identity = 82/290 (28.28%), Postives = 112/290 (38.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFER--KIISICDAVTCRSP----------ANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN------------PCLDKATCRNSYGSFD----------CFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCI-NSFGGFNC 3338
            C +GYQ + D C DINEC S  CH+ + C N PGS+ C C         + GC     EC  +      + C    CRSP          ANC +      C CP     D    C+ I EC  N             C+D  +  N+ G+            C C  G       GCV +  C+    C+   IC + I S  C+         ++ CI       S++LC QG   VCQ T  S    CP         C    EC+ ++ C E  TC+ +++G   C
Sbjct: 2380 CYEGYQLVNDVCEDINECLSQPCHSTAFCNNLPGSYSCQCPEGLIGDPLQAGCR-DPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHI-ECADNDDCSGEKACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCS--------TNRDCI-------SEQLCLQG---VCQGTCKSNS-SCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKC 2648          

HSP 10 Score: 54.6842 bits (130), Expect = 1.100e-6
Identity = 56/204 (27.45%), Postives = 81/204 (39.71%), Query Frame = 3
Query: 2634 SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ----GDNVVCQNTPGSFKCVCPVG 3233
            S  C  N+ C    G   C C N   + NP  GC  + +         ++C   +C   A C +      C+CP+GF+ +   GC D++EC   PC   A C N  G F+C C  G   + +  C  I    +SK C     CQ    + R  C  GY   + +           K LC     G   +C     +  C+CP+G
Sbjct: 1357 SPGCGENAHCEYGLGQSRCAC-NPGTFGNPYEGCGAQSK---------NVCQPNSCGPNAECRAVGNHISCLCPQGFSGNPYIGCQDVDECANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPI----ESKFCQDANKCQ---CNERVECPEGYSCQKGQ----------CKNLCSQASCGPRAICD----AGNCICPMG 1529          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Match: fbn2b (fibrillin 2b [Source:ZFIN;Acc:ZDB-GENE-090112-3])

HSP 1 Score: 147.132 bits (370), Expect = 1.106e-34
Identity = 109/275 (39.64%), Postives = 152/275 (55.27%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C DI+EC+S    C  N+ CIN PGS+ C CS   + L+P   C  +  EC   ++I ++C      S   C  + G   C+C  GF T   +  C+DI+EC+  PC    TC+N+ GS++C C  GF+ + +  C+DIDEC    S++C  NG C N +GS++C C +GY    D K C+DI+EC      CQ     CQN  GSF+C+CP G E +   CVDI+EC  + N C  G TC N+ G F C C  GFV +++ + C
Sbjct: 1798 GFSYNNILLICEDIDECNSGDNLCQRNANCINIPGSYRCECSAGFK-LSPSGACIDR-NEC---QEIPNVC------SHGECIDTQGSFRCLCHNGFKTTPDQTMCMDIDECDRQPC-GNGTCKNTVGSYNCLCFPGFELTHNNDCMDIDECSVHASQVCR-NGQCINNMGSFKCLCLDGYNLTPDGKNCVDINECVTLPGACQPG--TCQNLDGSFRCICPPGYEVQNDKCVDINECNVEPNICQFG-TCKNTPGSFQCICQPGFVLSDNGRRC 2056          

HSP 2 Score: 133.65 bits (335), Expect = 1.329e-30
Identity = 106/300 (35.33%), Postives = 145/300 (48.33%), Query Frame = 3
Query: 2511 RTTKELSQHLSKVFDITCKDVNCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEY-LNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            + +  L +H   V  +     +C  GYQ   D   C+DI+EC  +    ++ C N+ GS+ C CS  E Y L P+      I+EC        ICD         C++  G   C+C  GF   +    CID+NEC++NP  CL    C N+ GSF C C  G F      GC D+DEC   +  C  +  C N  GS++C C +G+E    K CID+DEC   +  C   N  C NTPGS++C C  G       C D+DEC  N    E   C+N+ GG+ C C  GF    D+K C+
Sbjct: 1165 QLSDNLCKHGQCVNMVGTYQCSCETGYQVTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCS--EGYALMPDLRTCADIDEC---EDTPDICDG------GQCTNIPGEYRCLCYDGFMASMDMRTCIDVNECDLNPNICL-HGDCENTKGSFICHCQLGYFVKKGSTGCTDVDECEIGAHNCDLHAACVNAPGSFKCRCRDGWEGDGIK-CIDVDECVTEEHNCN-PNAECLNTPGSYRCSCKEGFNGDGFSCSDMDECADNVNLCENGQCLNAPGGYRCECEMGFTPTEDSKACQ 1450          

HSP 3 Score: 125.176 bits (313), Expect = 6.064e-28
Identity = 99/270 (36.67%), Postives = 137/270 (50.74%), Query Frame = 3
Query: 2574 NCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C  GY     G +CID++EC       N  C NT GS++C C      L P+      ++ECS ++                C ++ G   C CP GFT   +  CID NEC+  P  C  +A+C NS GSF+C C KGF   +    C D+DEC  +  C     CQNM+G YRC C  GY ++ +   C+D +EC  ++      +  C NT GS+KCVCP G +++    GC D++EC   NN C  G  C N+ GG+ C C  GF
Sbjct: 2435 HCNVGYTNDFTGTSCIDMDECSQSPKPCNFLCKNTEGSYLCSCPRG-YILQPDGKTCKDLDECSTKQHNCQFL----------CVNTIGGFTCKCPSGFTQH-QTACIDNNECSNQPNICGSRASCLNSPGSFNCECQKGFSLDATGLNCDDVDECGGNHRCQHG--CQNMMGGYRCGCPQGYVQHYQWNQCVDENECTGNQVC---GSASCYNTLGSYKCVCPSGFDFEATAGGCQDVNECSMGNNPCSYG--CSNTDGGYLCGCPGGF 2685          

HSP 4 Score: 113.62 bits (283), Expect = 1.760e-24
Identity = 74/184 (40.22%), Postives = 102/184 (55.43%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D++  CID++EC  NPC++   C N+ GS+ C C +G+Q +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K CID DEC A+  +C   N +C N  GSFKC+C  G      G  C DIDEC  +  C  G  C+N+ G F C C  G   + D + C
Sbjct:  479 CECNMGYRQDVRGECIDVDECVSNPCVN-GDCVNTQGSYHCKCHEGYQGTPTKQACIDIDECIVNGVMCRNGRCVNTDGSFQCICNAGFEISPDGKNCIDHDEC-ATTNMCL--NGMCINEDGSFKCICKPGFALAPNGRYCTDIDECHTSGICMNGR-CVNTEGSFRCECPPGLAIDVDGRVC 657          

HSP 5 Score: 113.62 bits (283), Expect = 1.820e-24
Identity = 91/264 (34.47%), Postives = 128/264 (48.48%), Query Frame = 3
Query: 2616 DINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D+NEC     L  H   R  NT GSF C C +       E  C   I+EC+    +      V       C   +G E      GF   + + C+DI+EC  +P L +  TC N+ GS++C CP G Q S     C D++EC+ S     +G C NM+G+Y+C+C  GY+   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L    + C DIDEC+      +G  C N  G + C C  GF+ + D + C
Sbjct: 1033 DVNECKVFRGLCTHGTCR--NTIGSFKCRCDSGFALTMEERNCT-DIDECTISPDLCGHGVCVNTPGSFECECFDGYE-----SGFM--MMKNCMDIDECERDPLLCRGGTCLNTEGSYECDCPPGHQLSTEASACEDVNECQLSDNLCKHGQCVNMVGTYQCSCETGYQVTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDLRTCADIDECEDTPDICDGGQCTNIPGEYRCLCYDGFMASMDMRTC 1283          

HSP 6 Score: 105.531 bits (262), Expect = 4.707e-22
Identity = 93/278 (33.45%), Postives = 125/278 (44.96%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-PKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DINEC+ +     N +CIN+ GSF C+C  N  Y N   G +   ++ECS   K            P N  C ++ G   C CPRG+      + C D++EC+      +  C N+ G F C CP GF       C+D +EC +   IC     C N  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC  N  C   S C N+ G + C C  GF F      C+
Sbjct: 2400 GPGYTTDGR---DINECEVMPNLCKNGQCINSVGSFRCHC--NVGYTNDFTGTSCIDMDECSQSPK------------PCNFLCKNTEGSYLCSCPRGYILQPDGKTCKDLDECSTKQHNCQFLCVNTIGGFTCKCPSGFTQH-QTACIDNNECSNQPNICGSRASCLNSPGSFNCECQKGFSLDATGLNCDDVDECGGNHRCQHG----CQNMMGGYRCGCPQGYVQHYQWNQCVDENECTGNQVCGSAS-CYNTLGSYKCVCPSGFDFEATAGGCQ 2654          

HSP 7 Score: 98.5969 bits (244), Expect = 7.462e-20
Identity = 93/310 (30.00%), Postives = 135/310 (43.55%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDID----------------ECQASKKLCQGDNVVCQNTPGS---------------FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G++  G  CID++EC  +   C+ N+ C+NTPGS+ C C   E +    F C+  ++EC+         D V       C ++ G   C C  GFT  +  + C DI+ECN        +C+N  G F C C  G++     G C DI+EC D   C  NG+C N+ GSY C C   +E      GC+D                   C A   +       C +  G+               +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1355 CRDGWEGDGIKCIDVDECVTEEHNCNPNAECLNTPGSYRCSCK--EGFNGDGFSCS-DMDECA---------DNVNLCENGQCLNAPGGYRCECEMGFTPTEDSKACQDIDECNFQNICVFGSCQNLPGMFRCVCDYGYELDRSGGNCTDINECFDPVNCI-NGVCVNLPGSYLCNCPPDFELNPSGVGCVDTRVGNCFLDTLDRGDGGISCSAEIGVGVTRASCCCSLGGAWGNPCELCPSVNSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1651          

HSP 8 Score: 97.0561 bits (240), Expect = 1.760e-19
Identity = 71/185 (38.38%), Postives = 97/185 (52.43%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GF  D+  G  IDI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC     +C  N  C N+ GSYRC C  G++      CID +ECQ    +C   +  C +T GSF+C+C  G +    +  C+DIDEC +   C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1756 GFIIDIHTGKPIDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICEDIDECNSGDNLCQRNANCINIPGSYRCECSAGFKLSPSGACIDRNECQEIPNVCS--HGECIDTQGSFRCLCHNGFKTTPDQTMCMDIDECDRQ-PCGNG-TCKNTVGSYNCLCFPGFELTHNNDCMDIDE 1936          

HSP 9 Score: 96.6709 bits (239), Expect = 2.750e-19
Identity = 99/297 (33.33%), Postives = 129/297 (43.43%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CP---------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        C+NT GSF C C     YLN E      I+EC+     I IC       P  C ++ G   CVCP  +   +    C+D+              NE + N  + K  C  +Y     +      CP                GF   +H G  +DIDECR+      NG+C N IGS+RC C  G+ Y      C DIDEC +   LCQ  N  C N PGS++C C  G +    G C+D +ECQ+  N C  G  CI++ G F C C+ GF    D   C
Sbjct: 1610 DIDECQELPGLCQGGNCVNTFGSFQCECPAGY-YLNEETRICEDIDECTAH---IGIC------GPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNFNDTCENELSFN--MTKKMCCCAYNVGKAWNRPCEACPTPATSEYQLLCGNQAPGFIIDIHTGKPIDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICEDIDECNSGDNLCQ-RNANCINIPGSYRCECSAGFKLSPSGACIDRNECQEIPNVCSHGE-CIDTQGSFRCLCHNGFKTTPDQTMC 1892          

HSP 10 Score: 96.2857 bits (238), Expect = 3.785e-19
Identity = 102/296 (34.46%), Postives = 128/296 (43.24%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKE-------------GCIDINECNMNPCLDKAT-----CRNSYGSFDCFC-PKGFQYSVHFGCV------------DIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G NCID +EC +     N  CIN  GSF C C        P F  AP    C+     I  C          C ++ G   C CP G   D+               G I +  C+  P     T     C N    F   C P     S  F  V            DI+EC  D  IC  NGIC+N+ GSYRC C+ GYE     K C+DI+EC  ++ LC  DN +C+NTPGS+ C CP G  +K   + C DI+EC  +N C  G  C N  G FNC C+ G   ++    C
Sbjct:  572 GKNCIDHDECATTNMCLNGMCINEDGSFKCICK-------PGFALAPNGRYCT----DIDECHTSGICMNGRCVNTEGSFRCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGVCSR-PFPGAVTKSECCCANPEHGFGEPCQPCPAFNSAEFQAVCSSGPGITTDGRDINECALDPDICQ-NGICENLRGSYRCICNIGYESDASGKNCVDINECLVNRLLC--DNGMCRNTPGSYTCSCPKGFSFKADSETCEDINEC-DSNPCING-ICRNIAGSFNCECSHGSKLDSTNTVC 850          

HSP 11 Score: 95.5153 bits (236), Expect = 5.212e-19
Identity = 88/287 (30.66%), Postives = 129/287 (44.95%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            D  C KG+ ++ G  C DINEC+       N RC+NT GSF C C            C   + E+C  +       D   C  P        V+ C C  G  + +        +C   P       + +   F   CP+G   +     +       D++EC+  +    +G C+N IGS++C C +G+   +E++ C DIDEC  S  LC   + VC NTPGSF+C C  G E      K C+DIDEC+++     G TC+N+ G + C C  G   + +   CE
Sbjct:  898 DPACSKGFGRMKGLVCEDINECEVFPGVCTNGRCVNTQGSFRCECPEGLTLDGAGRTCVDVRSEQCYMKW------DEDECVEPL--PGRYRVDMCCCTVGAAWGV--------DCEACP-------KPNTNEFKTICPRGPGIANRGDILTGRPFYKDVNECKVFRGLCTHGTCRNTIGSFKCRCDSGFALTMEERNCTDIDECTISPDLC--GHGVCVNTPGSFECECFDGYESGFMMMKNCMDIDECERDPLLCRGGTCLNTEGSYECDCPPGHQLSTEASACE 1159          

HSP 12 Score: 93.5893 bits (231), Expect = 2.178e-18
Identity = 96/306 (31.37%), Postives = 138/306 (45.10%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDS-KICSGNGI-CQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKN 3497
            D+NEC ++     N  CINT GSF C C            C    +ECS    I + C   TC      S+  G   C C  GF       C DINEC +NP L    C N++GS++C CP G+     +  C D DEC +    C+  G+ C+N IG++ C C  G     D +GC+D++EC++   +C+  N  C NT GS++C C  G E    G   ID  +           C + ST  N+     C CN G  + +  + C L  T+   +    L  G  T     N  +++ ++ KN
Sbjct: 2131 DMNECVENPEICGNGLCINTDGSFRCECPFGYNLDYTGVNCV-DTDECS----IGNPCGNGTC------SNVPGGFECSCQEGFEPGPMMTCEDINECAVNPLLCAFRCVNTFGSYECMCPTGYVLRDDNRMCRDQDECAEGLDDCASRGMACKNQIGTFMCICPPGMTRRPDGEGCMDLNECRSKPGICK--NGRCVNTVGSYRCECNEGFEASATGTECIDNRKGFCFTEVLQTMCQQSSTNRNTVTKSECCCNGGRGWGSLCELCPLPGTIQ-YKKMCPLGPGYTTDGRDINECEVMPNLCKN 2422          

HSP 13 Score: 88.5817 bits (218), Expect = 7.649e-17
Identity = 91/308 (29.55%), Postives = 133/308 (43.18%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-------------FGCVDIDECR--------DSKICSGNG------ICQNMIG----SYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   E ++P+       +EC+     ++            C + +G   C+C  GF        C DI+EC+ +       C N+ GSF C CP G    V              +G + +  C          S+ C  N        CQ         ++  C +G     D    DI+EC     +CQ  N +C+N  GS++C+C +G E    G  CVDI+EC  N    +   C N+ G + C+C KGF F  D++ CE
Sbjct:  520 CHEGYQGTPTKQACIDIDECIVNGVMCRN-GRCVNTDGSFQCICNAGFE-ISPDGKNCIDHDECATTNMCLN----------GMCINEDGSFKCICKPGFALAPNGRYCTDIDECHTSGICMNGRCVNTEGSFRCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGVCSRPFPGAVTKSECCCANPEHGFGEPCQPCPAFNSAEFQAVCSSGPGITTDG--RDINECALDPDICQ--NGICENLRGSYRCICNIGYESDASGKNCVDINECLVNRLLCDNGMCRNTPGSYTCSCPKGFSFKADSETCE 811          

HSP 14 Score: 82.8037 bits (203), Expect = 3.727e-15
Identity = 78/248 (31.45%), Postives = 112/248 (45.16%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNN-----------EEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--------CSSSNGVEF---CVCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVD 3263
            G  C DI+EC +     N RC+NT GSF C C              + ++      A K+  CS             C +P +        C + N  EF   C    G T D +    DINEC ++P +     C N  GS+ C C  G++       CVDI+EC  +++   NG+C+N  GSY C+C  G+ +  D + C DI+EC ++  +    N +C+N  GSF C C  G  L+     CVD
Sbjct:  613 GRYCTDIDECHTSGICMNGRCVNTEGSFRCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGVCSRPFPGAVTKSECCCANPEHGFGEPCQPCPAFNSAEFQAVCSSGPGITTDGR----DINECALDPDICQNGICENLRGSYRCICNIGYESDASGKNCVDINECLVNRLLCDNGMCRNTPGSYTCSCPKGFSFKADSETCEDINECDSNPCI----NGICRNIAGSFNCECSHGSKLDSTNTVCVD 852          

HSP 15 Score: 81.2629 bits (199), Expect = 1.170e-14
Identity = 101/350 (28.86%), Postives = 135/350 (38.57%), Query Frame = 3
Query: 2457 ITINND-VDIPR-SIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATC-----RNSY---------------------------GSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +T NND +DI   S++   V    +   ++   F   C D   G      G NC+DINEC +L  AC   + C N  GSF C C    E  N +      I EC+ E  I              C ++ G   C+C  GF   D    C D  E       +   C     RN+                             +F   CP G +      G  D++EC ++    GNG+C N  GS+RC C  GY        C+D DEC          N  C N PG F+C C  G E      C DI+EC  N        C+N+FG + C C  G+V  +D + C 
Sbjct: 1925 LTHNNDCMDIDECSVHASQVCRNGQCINNMGS-FKCLCLD---GYNLTPDGKNCVDINECVTLPGACQPGT-CQNLDGSFRCICPPGYEVQNDK---CVDINECNVEPNICQF---------GTCKNTPGSFQCICQPGFVLSDNGRRCFDTRESFCFTRFEAGKCSVPKPRNTTKAKCCCSLLPGEGWGDPCELCPRDTEEAAFHTLCPYGHEAVPKPEGREDMNECVENPEICGNGLCINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC---GNGTCSNVPGGFECSCQEGFEPGPMMTCEDINECAVNPLLC-AFRCVNTFGSYECMCPTGYVLRDDNRMCR 2253          

HSP 16 Score: 80.1073 bits (196), Expect = 2.473e-14
Identity = 89/309 (28.80%), Postives = 133/309 (43.04%), Query Frame = 3
Query: 2610 CIDINEC--DSLACHNNSRCINTPGSFVCYCS------NNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGY------EYVEDKGCI-----------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            C DINEC  + L C    RC+NT GS+ C C       ++      +  CA  +++C+                   C +  G   C+CP G T     EGC+D+NEC   P + K   C N+ GS+ C C +GF+ S         EC D++           +C  +   +N +    C C+ G       E     G I                 DI+EC+    LC+  N  C N+ GSF+C C VG   ++    C+D+DEC Q+   P    C N+ G + C+C +G++   D K C +LD+
Sbjct: 2211 CEDINECAVNPLLCA--FRCVNTFGSYECMCPTGYVLRDDNRMCRDQDECAEGLDDCASRGMA--------------CKNQIGTFMCICPPGMTRRPDGEGCMDLNECRSKPGICKNGRCVNTVGSYRCECNEGFEASATG-----TECIDNRKGFCFTEVLQTMCQQSSTNRNTVTKSECCCNGGRGWGSLCELCPLPGTIQYKKMCPLGPGYTTDGRDINECEVMPNLCK--NGQCINSVGSFRCHCNVGYTNDFTGTSCIDMDECSQSPK-PCNFLCKNTEGSYLCSCPRGYILQPDGKTCKDLDE 2495          

HSP 17 Score: 76.2554 bits (186), Expect = 4.173e-13
Identity = 71/235 (30.21%), Postives = 100/235 (42.55%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCID 3137
            PR  I Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC +    C + + C+N+PGSF C C            C   ++EC               R    C +  G   C CP+G+    +   C+D NEC  N     A+C N+ GS+ C CP GF + +   GC D++EC   +  CS    C N  G Y C C  G+       CI+
Sbjct: 2478 PRGYILQPDGKTCKDLDECSTKQHNCQFLCVNTIGGFTCK---CPSGFTQHQTACIDNNECSNQPNICGSRASCLNSPGSFNCECQKGFSLDATGLNCD-DVDECGGNH-----------RCQHGCQNMMGGYRCGCPQGYVQHYQWNQCVDENECTGNQVCGSASCYNTLGSYKCVCPSGFDFEATAGGCQDVNECSMGNNPCSYG--CSNTDGGYLCGCPGGFYRAGQGHCIN 2695          

HSP 18 Score: 73.1738 bits (178), Expect = 3.387e-12
Identity = 102/350 (29.14%), Postives = 144/350 (41.14%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQ--KIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEEC---SFERK----------IISICDAVTCRSPAN--------CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTP----GSFKCVCPVG-----LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            Q+L  +F   C       GY+  + G NC DINEC D + C N   C+N PGS++C C  + E LNP   GC   ++  C   + +R            + +  A  C S           C S N  E+  +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P      ++ +C        ++      +DIDEC++         CIN  G F C C  GF +NN    CE
Sbjct: 1467 QNLPGMFRCVCD-----YGYELDRSGGNCTDINECFDPVNCINGV-CVNLPGSYLCNCPPDFE-LNPSGVGCVDTRVGNCFLDTLDRGDGGISCSAEIGVGVTRASCCCSLGGAWGNPCELCPSVNSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECTAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNFNDTCENELSFNMTKKMCCCAYNVGKAWNRPCEACPTPATSEYQLLCGNQAPGFIIDIHTGKPIDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICE 1809          

HSP 19 Score: 66.6254 bits (161), Expect = 3.789e-10
Identity = 46/113 (40.71%), Postives = 56/113 (49.56%), Query Frame = 3
Query: 3051 NGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NG C     SYRC C+ GY       CID+DEC  S     GD   C NT GS+ C C  G +    ++ C+DIDEC  N        C+N+ G F C CN GF  + D K C
Sbjct:  467 NGRCIPTPTSYRCECNMGYRQDVRGECIDVDEC-VSNPCVNGD---CVNTQGSYHCKCHEGYQGTPTKQACIDIDECIVNGVMCRNGRCVNTDGSFQCICNAGFEISPDGKNC 575          

HSP 20 Score: 63.5438 bits (153), Expect = 2.687e-9
Identity = 39/100 (39.00%), Postives = 53/100 (53.00%), Query Frame = 3
Query: 3093 CHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+   +    C D+DECQA   LC G N  C NT GS++C CP G    E  + C DIDEC   +   +G  C N+ G + CTC +G++ + D   C
Sbjct:  230 CRRGFIPNIRTGACQDVDECQAVPGLCAGGN--CINTVGSYECKCPAGHRQSETNQKCEDIDECATISGVCDGGECTNTAGSYVCTCPRGYITSTDGSRC 327          

HSP 21 Score: 62.7734 bits (151), Expect = 5.177e-9
Identity = 91/330 (27.58%), Postives = 124/330 (37.58%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDIN------ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFK---CVCPVGLEYKEKGCVDIDECQQNNT------CPEG-------------------------------STCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+DINEC  + L C +N  C NTPGS+ C C     +      C   I EC     I  IC  +      NC  S+G +          D  +G   +N      E N+N    K+ C ++ G+           D  C KGF       C DI+EC        NG C N  GS+RC C  G       + C+D+   Q   K  + + V  +  PG ++   C C VG  +     VD + C + NT      CP G                                TC N+ G F C C+ GF    + + C
Sbjct:  755 CNIGYESDASGKNCVDINECLVNRLLC-DNGMCRNTPGSYTCSCPKGFSFKADSETCE-DINECDSNPCINGICRNIA--GSFNCECSHGSKL-DSTNTVCVDSMKGTCWLNIQDGRCEVNINGATLKSECCSTLGAAWGSPCERCEIDPACSKGFGRMKGLVCEDINECEVFPGVCTNGRCVNTQGSFRCECPEGLTLDGAGRTCVDVRSEQCYMKWDEDECV--EPLPGRYRVDMCCCTVGAAWG----VDCEACPKPNTNEFKTICPRGPGIANRGDILTGRPFYKDVNECKVFRGLCTHGTCRNTIGSFKCRCDSGFALTMEERNC 1073          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Match: fbn2a (fibrillin 2a [Source:ZFIN;Acc:ZDB-GENE-090112-4])

HSP 1 Score: 143.665 bits (361), Expect = 1.405e-33
Identity = 103/264 (39.02%), Postives = 135/264 (51.14%), Query Frame = 3
Query: 2610 CIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC S    C  N+ CIN PG + C CS+    L P   C  + E    E   +       CR   +C    G   C+C  GFT    +  C+DINEC+  PC    TC+NS GSF+C C  GF+ + +  C DIDEC    S++C  NG C N +GS++C C  GYE   D K C+D++EC     +C      C N  GSF+CVCP G   +   CVD++EC +        TC NS G F C C  GFV + D + C
Sbjct: 1763 CEDIDECSSREPVCQRNADCINRPGGYQCECSDGY-ILTPNGDCNDRNE--CLETPSV-------CRH-GDCVDIPGGYECMCHTGFTVTPNRRMCVDINECSHQPC-GNGTCKNSVGSFNCICHHGFELATNNYCTDIDECAVFHSQLCR-NGRCVNNVGSFQCLCREGYELTFDGKTCMDVNECTIIPGICAPG--TCLNLDGSFRCVCPDGYIVQSDHCVDVNECSEEPGICTYGTCFNSLGSFECVCKPGFVLSEDKRRC 2011          

HSP 2 Score: 117.857 bits (294), Expect = 1.049e-25
Identity = 99/298 (33.22%), Postives = 143/298 (47.99%), Query Frame = 3
Query: 2541 SKVFDITCKDVNCGKGYQKIGD-------NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD--LKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ++ FD  C     G G+   GD       +  DINEC    S+  H   +C NT GSF C C +       E  C   I+EC     +              C ++ G   C C  G+     + + C+DI+EC  N  L +  TC N+ GS+ C CP G   SV    C D++EC  S     NG C N++G+Y+C+C++GY+   D KGC DIDEC      C+   + C N+ GS+ C C  G  L   ++ C D++EC++     EG  C N  G ++C C +GF+ + D K C
Sbjct:  961 TRQFDTLCPR---GPGFANKGDFINGAIPSSSDINECKVFQSICVH--GKCRNTIGSFRCRCDSGFALDTNEKNCT-DIDECMISPDVCG---------HGTCVNTLGSFQCDCFPGYESGIMMMKNCMDIDECERNLSLCNGGTCENTDGSYKCVCPPGHHLSVDGSACEDVNECDLSSTLCPNGWCVNLVGTYQCSCNSGYQTTPDWKGCTDIDECTIENGGCE---LYCSNSEGSYSCSCGQGYSLTPDQRTCSDVNECEEVLDICEGGQCTNVPGAYHCLCYEGFMASVDMKTC 1240          

HSP 3 Score: 110.923 bits (276), Expect = 1.273e-23
Identity = 106/371 (28.57%), Postives = 163/371 (43.94%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFV------------FNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLN 3620
            +C  GY     G  C+D++EC       N  C NT GS+ C C       N    C   ++EC   R               +C ++ G   C CP GF+   +     I+EC  ++ PC  +  C+NS GSF C CP+GF   S+   C D+DEC RD +   G   CQN+ G +RC+C  GY ++ +   C+D +EC  +       +  C NT GSFKC CP G  +      C D+DEC    N C  G  C N+ GGF C C  G+             + +D     +   L  ++P  D V     S  + N +++V+  ++++       ++   +S        +    V++I E   N ++
Sbjct: 2390 HCKPGYIASTAGTACVDVDECGMSPKPCNFICKNTEGSYACSCPRGYTLQNDNRSCK-DVDECQSRRHNCQF----------SCMNTIGGFTCKCPAGFSQHHR---AYIDECLSDLTPCGLQGACQNSVGSFSCECPQGFSLDSLGHNCDDVDECARDHRCQFG---CQNVAGGFRCSCPQGYTQHQQWNQCVDDNECLNADNC---GSASCFNTLGSFKCGCPSGFTFDPASTSCEDVDECGSFMNPCRYG--CSNTQGGFACGCPAGYYRAGQGRNLICADWTDDNPLRLVVAILKKLRPALDRV---KCSSWLLNNIEVVR--VQSVCVSIPANTVVFPESLPHFRPISHHLPIVLFIPENSGNFID 2733          

HSP 4 Score: 109.383 bits (272), Expect = 3.159e-23
Identity = 89/278 (32.01%), Postives = 128/278 (46.04%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC          C N+ GS+ C C      L P+      + EC    +++ IC+         C++  G   C+C  GF  + D+K  CID+NEC +N  +     C N+ GSF C C  G+       GC D++EC  +S  C  +  C N  G Y C+C +G+ + +   C D+DEC     +C  D   C NT GS+ C C  G       C D+DEC ++    E   C+N  G + C C  GF   ++ K C 
Sbjct: 1143 SCNSGYQTTPDWKGCTDIDECTIENGGCELYCSNSEGSYSCSCGQGYS-LTPDQRTCSDVNEC---EEVLDICEG------GQCTNVPGAYHCLCYEGFMASVDMKT-CIDVNECELNDNICAFGECENTKGSFICHCDMGYAVKKGTSGCTDVNECEINSHNCDTHATCVNTPGHYHCSCGDGW-FGDGVKCADVDECANRTSVCSAD-AECVNTAGSYHCECSDGFIGDGVICSDLDECAEDVDLCENGQCLNVPGSYRCECEMGFTHTSNRKTCR 1407          

HSP 5 Score: 107.842 bits (268), Expect = 9.518e-23
Identity = 99/291 (34.02%), Postives = 134/291 (46.05%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-PKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLNVVQP 3416
            G GY   G    DI+EC  +   C N + CIN  GS+ C+C     Y+    G A   ++EC    K            P N  C ++ G   C CPRG+T  +    C D++EC       + +C N+ G F C CP GF Q+   +    IDEC  D   C   G CQN +GS+ C C  G+        C D+DEC A    CQ     CQN  G F+C CP G    ++   CVD +EC   + C   S C N+ G F C C  GF F+  +  CE +D+  + + P
Sbjct: 2355 GHGYAIDG---TDIDECKLIPDVCANGA-CINMMGSYRCHCKPG--YIASTAGTACVDVDECGMSPK------------PCNFICKNTEGSYACSCPRGYTLQNDNRSCKDVDECQSRRHNCQFSCMNTIGGFTCKCPAGFSQHHRAY----IDECLSDLTPCGLQGACQNSVGSFSCECPQGFSLDSLGHNCDDVDEC-ARDHRCQ---FGCQNVAGGFRCSCPQGYTQHQQWNQCVDDNECLNADNCGSAS-CFNTLGSFKCGCPSGFTFDPASTSCEDVDECGSFMNP 2618          

HSP 6 Score: 100.908 bits (250), Expect = 1.473e-20
Identity = 69/183 (37.70%), Postives = 92/183 (50.27%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  GF  D +  C D++EC  +PC +   C N+ GS+ C C  GF         DIDEC  + +   NG C N  GS++C C++G++   D + C+D DEC A+     G   +C N  GSFKCVC  G      G  C DIDEC+    C  G  C+N+ G F C C  G     D + C
Sbjct:  452 CDCNTGFKEDNRGECSDVDECEFDPCAN-GNCINTPGSYYCRCHTGFLRV-----PDIDECLQNGVLCKNGRCLNTEGSFQCICNSGFKLSPDGRNCVDHDECAATNMCLNG---MCINEDGSFKCVCKPGFTLNSSGRYCTDIDECRTPGVCMNGR-CVNTQGSFRCECFAGLTVGIDGRTC 624          

HSP 7 Score: 99.3673 bits (246), Expect = 3.686e-20
Identity = 98/310 (31.61%), Postives = 134/310 (43.23%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYC--------SNNEEYLNPEFGCAPKIE--ECSFERKIISICDAVTCRSPANCSSSNGVEFC----------VCP--RGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQN--NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQP 3416
            C  GY    D+C+D+NEC +         C N+ GSF C C             Y   E  C  + E  +CS  +   +      C   A     +  E C          +CP   G    + +  +D+NEC  NP  C++   C N  GSF C CP G+        C DIDEC     C GNG C N+IG++ C C  G+E      C D++EC  +  LC      C NT GS++C CP G   +E G  C D+DEC ++  N    G  C N  G F C C  G +     + C+ D+   + QP
Sbjct: 1958 CPDGYIVQSDHCVDVNECSEEPGICTYGTCFNSLGSFECVCKPGFVLSEDKRRCYDTRESFCFTRFENSKCSVPQAFNTTKAKCCCSIMAKEGWGDPCELCPKEHDAAFQDLCPFGHGIIPGVGDTRVDLNECVENPEICVN-GRCINMDGSFRCECPAGYTLDYTGTHCDDIDECSVRNPC-GNGTCSNVIGAFECLCEEGFEPGPMMSCEDVNECGQNPLLCA---FRCINTFGSYECSCPSGYTLREDGRMCQDVDECAEDLHNCDSRGMECSNLIGTFMCVCPAGMIRRMSDEACQ-DENECLTQP 2261          

HSP 8 Score: 97.4413 bits (241), Expect = 1.417e-19
Identity = 91/309 (29.45%), Postives = 139/309 (44.98%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID-------IDECQASKKLCQGD------------------NVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +CG G+   G  C D++EC   +  C  ++ C+NT GS+ C CS+   ++     C+  ++EC+ +   + +C+         C +  G   C C  GFT+   ++ C DI+EC+        TC+N  G F C C +G++     G C D+DEC DS  C  NG C+N  GSY+C C   +E      GC+D       ++    SK  C  +                     C + P      +  +CP G  +K         DIDECQ+     +G  C+N+FG F C C  GF  N  ++ CE
Sbjct: 1311 SCGDGWFGDGVKCADVDECANRTSVCSADAECVNTAGSYHCECSDG--FIGDGVICS-DLDECAED---VDLCEN------GQCLNVPGSYRCECEMGFTHTSNRKTCRDIDECSFQNICVFGTCQNIPGMFRCICDEGYELDRTGGNCTDVDECLDSLNCV-NGHCENTAGSYQCNCPIDFELNPTGIGCVDTRVGNCFLEVAYNSKLACSAEIGFGVSRSSCCCSLGRVWGYPCDHCPTLNTSEYNTLCPGGEGFKPNPVTIILEDIDECQELPGVCQGGNCVNTFGSFQCECPAGFYLNTQSRICE 1606          

HSP 9 Score: 92.8189 bits (229), Expect = 4.103e-18
Identity = 103/338 (30.47%), Postives = 144/338 (42.60%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN---CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECR-----------------------------DSKICSGN------------GIC----QNMIGSYRCACHNGYEYVEDKGC--------------IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+++  DN   C D++EC+   C N + CINTPGS+ C C          F   P I+EC        + + V C++   C ++ G   C+C  GF        C+D +EC   N CL+   C N  GSF C C  GF   S    C DIDECR                             D + C               G+C    Q  +   +C C N  +Y   + C               DI+EC     +C   N VC+N  G+F+CVC +G E  + G  C+DIDEC  N+   +   C N  G ++CTC  GF+F +DT+ CE
Sbjct:  453 DCNTGFKE--DNRGECSDVDECEFDPCANGN-CINTPGSYYCRCHTG-------FLRVPDIDEC--------LQNGVLCKN-GRCLNTEGSFQCICNSGFKLSPDGRNCVDHDECAATNMCLN-GMCINEDGSFKCVCKPGFTLNSSGRYCTDIDECRTPGVCMNGRCVNTQGSFRCECFAGLTVGIDGRTCVDTHMRSTCYGAVKRGLCLRPFQRAVTKSQCCCAN-PDYAFGEPCHPCPAKHSGTAITHADINECALDPDICS--NGVCENLRGNFRCVCNIGYESDQSGRNCLDIDECVVNSLLCDNGLCRNVPGSYSCTCPAGFIFRHDTETCE 767          

HSP 10 Score: 92.0485 bits (227), Expect = 7.209e-18
Identity = 57/134 (42.54%), Postives = 74/134 (55.22%), Query Frame = 3
Query: 2967 FCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
             CP G  Y++     DIDEC+       NG C NM+GSYRC C  GY        C+D+DEC  S K C   N +C+NT GS+ C CP G  L+   + C D+DECQ + + C    +C+N+ GGF C C  GF
Sbjct: 2351 LCPYGHGYAIDG--TDIDECKLIPDVCANGACINMMGSYRCHCKPGYIASTAGTACVDVDECGMSPKPC---NFICKNTEGSYACSCPRGYTLQNDNRSCKDVDECQSRRHNCQ--FSCMNTIGGFTCKCPAGF 2477          

HSP 11 Score: 90.8929 bits (224), Expect = 1.425e-17
Identity = 88/284 (30.99%), Postives = 121/284 (42.61%), Query Frame = 3
Query: 2598 IGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDEC-RDSKICSGNGI-CQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG----------LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLN 3404
            +GD  +D+NEC ++     N RCIN  GSF C C            C   I+ECS                   CS+  G   C+C  GF       C D+NEC  NP L    C N++GS++C CP G+        C D+DEC  D   C   G+ C N+IG++ C C  G    + D+ C D +EC     +C+  N  C NT GS+ C C VG          L+Y++  C        +  C   S+         C CN G  + +  + C L  T N
Sbjct: 2080 VGDTRVDLNECVENPEICVNGRCINMDGSFRCECPAGYTLDYTGTHCD-DIDECSVRNPC----------GNGTCSNVIGAFECLCEEGFEPGPMMSCEDVNECGQNPLLCAFRCINTFGSYECSCPSGYTLREDGRMCQDVDECAEDLHNCDSRGMECSNLIGTFMCVCPAGMIRRMSDEACQDENECLTQPGVCE--NGRCVNTVGSYTCECGVGFKTDSSATQCLDYRKGFCFTE---VLHTMCQMSSSSRVPVSRSQCCCNGGHGWGDQCELCPLPATAN 2347          

HSP 12 Score: 90.8929 bits (224), Expect = 1.524e-17
Identity = 88/291 (30.24%), Postives = 130/291 (44.67%), Query Frame = 3
Query: 2568 DVNCGKGY-QKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEY-LNPEFGCAPKIEEC--SFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV--------DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            D+ C +G+ Q  G +C D+NEC+     CHN   C+NT GSF C C  +    +        + E C  ++E  +        C  P        ++ C C  G  +          +C++ P             FD  CP+G  ++     +        DI+EC+  +    +G C+N IGS+RC C +G+     +K C DIDEC  S  +C   +  C NT GSF+C C  G E      K C+DIDEC++N +   G TC N+ G + C C  G   + D   CE
Sbjct:  854 DLVCERGFAQSRGASCEDVNECEVFPGVCHN-GHCVNTRGSFKCQCPESLTLDVTGRLCVDVRSEPCFLTYEEDV--------CTQPV--PGRFRMDMCCCTVGLAWG--------KDCDLCP-------EPGTRQFDTLCPRGPGFANKGDFINGAIPSSSDINECKVFQSICVHGKCRNTIGSFRCRCDSGFALDTNEKNCTDIDECMISPDVC--GHGTCVNTLGSFQCDCFPGYESGIMMMKNCMDIDECERNLSLCNGGTCENTDGSYKCVCPPGHHLSVDGSACE 1116          

HSP 13 Score: 88.9669 bits (219), Expect = 5.617e-17
Identity = 69/187 (36.90%), Postives = 95/187 (50.80%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECN--MNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GF  D+  G   DI+EC+  +  C     C N  GSF C CP GF Y+ +   C DIDEC   + +C  N  C N  G Y+C C +GY    +  C D +EC  +  +C+ GD   C + PG ++C+C  G       + CVDI+EC     C  G TC NS G FNC C+ GF    +    ++D+
Sbjct: 1711 GFWIDIATGKPKDIDECHEILGVCA-HGVCINHMGSFHCECPTGFLYNDLLLICEDIDECSSREPVCQRNADCINRPGGYQCECSDGYILTPNGDCNDRNECLETPSVCRHGD---CVDIPGGYECMCHTGFTVTPNRRMCVDINECSHQ-PCGNG-TCKNSVGSFNCICHHGFELATNNYCTDIDE 1891          

HSP 14 Score: 77.7962 bits (190), Expect = 1.539e-13
Identity = 95/310 (30.65%), Postives = 129/310 (41.61%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINE-----------CNMNPCLD--KATC------RNSYGSFDCFCPK----GFQYSVHFGC----------VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GY+    G  C+D+NEC  +   C   + C+N  GSF C C +   Y+     C   + ECS E  I +            C +S G   CVC  GF   + K  C D  E           C++    +  KA C      +  +G     CPK     FQ    FG           VD++EC ++     NG C NM GS+RC C  GY        C DIDEC          N  C N  G+F+C+C  G E      C D++EC QN        CIN+FG + C+C  G+    D + C+
Sbjct: 1916 CREGYELTFDGKTCMDVNECTIIPGICAPGT-CLNLDGSFRCVCPDG--YIVQSDHCV-DVNECSEEPGICTY---------GTCFNSLGSFECVCKPGFVLSEDKRRCYDTRESFCFTRFENSKCSVPQAFNTTKAKCCCSIMAKEGWGDPCELCPKEHDAAFQDLCPFGHGIIPGVGDTRVDLNECVENPEICVNGRCINMDGSFRCECPAGYTLDYTGTHCDDIDECSVRNPC---GNGTCSNVIGAFECLCEEGFEPGPMMSCEDVNECGQNPL-LCAFRCINTFGSYECSCPSGYTLREDGRMCQ 2208          

HSP 15 Score: 67.3958 bits (163), Expect = 2.203e-10
Identity = 101/334 (30.24%), Postives = 135/334 (40.42%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKIIS--ICDA----VTCRSPANCS-------------SSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDE------------------------CQASKKLCQGDNVVCQNTP----GSFKCVCP----VGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GY+  + G NC D++EC DSL C N   C NT GS+ C C  + E LNP   GC   ++  C  E    S   C A       RS   CS             + N  E+  +CP G  +      I   DI+EC   P +     C N++GSF C CP GF  +     C DIDEC  S    G G C N +G+Y C C   Y  V     C+D+ +                        C  S  + +  N  C   P      +  +C       ++       DIDEC +    C  G  CIN  G F+C C  GF++N+    CE
Sbjct: 1434 CDEGYELDRTGGNCTDVDECLDSLNCVN-GHCENTAGSYQCNCPIDFE-LNPTGIGCVDTRVGNCFLEVAYNSKLACSAEIGFGVSRSSCCCSLGRVWGYPCDHCPTLNTSEYNTLCPGGEGFKPNPVTIILEDIDECQELPGVCQGGNCVNTFGSFQCECPAGFYLNTQSRICEDIDECVLSLGVCGPGTCYNTLGNYTCVCPQDYIQVNGGHRCMDMRKSLCYRSYNGSACEHQLNFNITHRLCCCSYNVGKAWNKPCHACPIPGTDDYSSLCGSVTGFWIDIATGKPKDIDECHEILGVCAHG-VCINHMGSFHCECPTGFLYNDLLLICE 1764          

HSP 16 Score: 63.929 bits (154), Expect = 2.062e-9
Identity = 43/111 (38.74%), Postives = 54/111 (48.65%), Query Frame = 3
Query: 3051 NGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NG C  M   YRC C+ G++      C D+DEC+     C   N  C NTPGS+ C C  G         DIDEC QN    +   C+N+ G F C CN GF  + D + C
Sbjct:  440 NGRCIPMESDYRCDCNTGFKEDNRGECSDVDECEFDP--CANGN--CINTPGSYYCRCHTGFLRVP----DIDECLQNGVLCKNGRCLNTEGSFQCICNSGFKLSPDGRNC 542          

HSP 17 Score: 61.6178 bits (148), Expect = 1.146e-8
Identity = 38/96 (39.58%), Postives = 50/96 (52.08%), Query Frame = 3
Query: 3126 GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNV 3407
             C D+DEC+    LC+G    C NT GS++C CPVG +       C D+DEC       E   CINS G F+C C  G+V + D   C LD+   +
Sbjct:  221 ACQDVDECKVVPDLCKGGR--CINTLGSYQCTCPVGYQQHVSILKCEDVDECSTIERACEAGHCINSAGSFSCVCPSGYVLSADRTRC-LDQHTGI 313          

HSP 18 Score: 57.7658 bits (138), Expect = 1.762e-7
Identity = 86/335 (25.67%), Postives = 129/335 (38.51%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC---IDINECNMNPCLDKATCRN--------SYGS------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI------------------------DECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKG---------CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+  + G NC+DI+EC  +SL C +N  C N PGS+ C C     + +    C   + EC     +  +C            +S G  +C C +G   D         +K  C   +    C +N  ++ AT R+        ++GS       D  C +GF  S    C D++EC        NG C N  GS++C C       V  + C+D+                        D C  +  L  G +      PG+  F  +CP G  +  KG           DI+EC+   +      C N+ G F C C+ GF  + + K C
Sbjct:  711 CNIGYESDQSGRNCLDIDECVVNSLLC-DNGLCRNVPGSYSCTCPAGFIFRHDTETCE-DVNECVSSPCVNGVC-----------KNSAGSFYCECSQGSKLDSTGLLCVDSVKGSCWLTLQDGRCEVN--INGATLRSQCCATLGLAWGSPCEPCQTDLVCERGFAQSRGASCEDVNECEVFPGVCHNGHCVNTRGSFKCQCPESLTLDVTGRLCVDVRSEPCFLTYEEDVCTQPVPGRFRMDMCCCTVGLAWGKDCDLCPEPGTRQFDTLCPRGPGFANKGDFINGAIPSSSDINECKVFQSICVHGKCRNTIGSFRCRCDSGFALDTNEKNC 1030          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Match: hmcn2 (hemicentin 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9765])

HSP 1 Score: 140.969 bits (354), Expect = 9.403e-33
Identity = 97/284 (34.15%), Postives = 132/284 (46.48%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV--CPRGFTYDL-KEGCIDINECN---MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRC--ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG----CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C KG+       C D +EC  DS   H+   C N  G F C C +    ++ E      I+ECS    +        C     C ++ G   C   C  GF   L    C D++EC    ++PC  +  C N+ GSF C C  G+Q   H  C+DI+EC    +C  +  C+N  G Y+C  +C  G    E+  C+DIDECQ    +C+   + CQNT G + CVCP G  Y+ +G    C+DIDEC Q  + P    C N  G F C C  G V   D + C
Sbjct: 3677 CPKGFILDTASYCADEDECAVDSPCSHS---CNNNMGGFSCACPSGFT-ISTESNSCQDIDECSQGSHL--------CHPNQQCVNTVGTYRCQAKCGLGFKPSLVGTSCEDVDECQESAVSPC--QQQCFNTLGSFRCTCHPGYQLVGH-RCLDINECL-RNVCPAHQQCRNTDGGYQCFDSCPAGMTAAENGVCVDIDECQDGSHMCRYSQI-CQNTIGGYGCVCPRG--YRSQGVGRPCLDIDECAQVPS-PCAFQCRNVPGSFRCVCPPGTVLLGDGRSC 3940          

HSP 2 Score: 136.346 bits (342), Expect = 2.381e-31
Identity = 80/225 (35.56%), Postives = 117/225 (52.00%), Query Frame = 3
Query: 2787 DAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNMNP--CLDKATCRNSYGSFDC--FCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQPKA 3422
            D     SP +  C+++ G   C CP GFT   +   C DI+EC+     C     C N+ G++ C   C  GF+ S V   C D+DEC++S +      C N +GS+RC CH GY+ V  + C+DI+EC   + +C      C+NT G ++C   CP G+   E G CVDIDECQ  ++ C     C N+ GG+ C C +G+      + C ++D+   V  P A
Sbjct: 3693 DECAVDSPCSHSCNNNMGGFSCACPSGFTISTESNSCQDIDECSQGSHLCHPNQQCVNTVGTYRCQAKCGLGFKPSLVGTSCEDVDECQESAVSPCQQQCFNTLGSFRCTCHPGYQLVGHR-CLDINECL--RNVCPAHQ-QCRNTDGGYQCFDSCPAGMTAAENGVCVDIDECQDGSHMCRYSQICQNTIGGYGCVCPRGYRSQGVGRPCLDIDECAQVPSPCA 3913          

HSP 3 Score: 135.191 bits (339), Expect = 5.907e-31
Identity = 95/293 (32.42%), Postives = 124/293 (42.32%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGN--------------------------------GICQNMIGSYR---CACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNC 3338
            C  GYQ +G  C+DINEC    C  + +C NT G + C+ S        E G    I+EC     +        CR    C ++ G   CVCPRG+ +  +   C+DI+EC   P      CRN  GSF C CP G   +V  G        D + C+G                                  +    +GS R     C  GY   +D  CIDIDEC A +K CQ +   C+NT GS++C CP G +    G  C DIDEC  Q   C     C N+ GG+ C
Sbjct: 3803 CHPGYQLVGHRCLDINECLRNVCPAHQQCRNTDGGYQCFDSCPAGMTAAENGVCVDIDECQDGSHM--------CRYSQICQNTIGGYGCVCPRGYRSQGVGRPCLDIDECAQVPSPCAFQCRNVPGSFRCVCPPG---TVLLG--------DGRSCAGLERGHIFTNSTRVQARLRPQLVSALERPYLTRLSVLPEHLGSSRRPRHECPVGYT-SKDGTCIDIDEC-AFRKPCQHE---CRNTIGSYQCTCPPGYQLLSNGRTCKDIDECTVQGVQCGPNQMCFNTRGGYQC 4071          

HSP 4 Score: 90.5077 bits (223), Expect = 2.005e-17
Identity = 69/212 (32.55%), Postives = 94/212 (44.34%), Query Frame = 3
Query: 2577 CGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-------------------PKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRC---ACHNGYEYVEDKG 3128
            C +GY  Q +G  C+DI+EC  +      +C N PGSF C C      L     CA                   P++   + ER  ++    +    P +  SS       CP G+T   K+G CIDI+EC    PC  +  CRN+ GS+ C CP G+Q  S    C DIDEC    + C  N +C N  G Y+C    C N Y+   + G
Sbjct: 3886 CPRGYRSQGVGRPCLDIDECAQVPSPCAFQCRNVPGSFRCVCPPGTVLLGDGRSCAGLERGHIFTNSTRVQARLRPQLVS-ALERPYLTRLSVL----PEHLGSSRRPRH-ECPVGYTS--KDGTCIDIDECAFRKPC--QHECRNTIGSYQCTCPPGYQLLSNGRTCKDIDECTVQGVQCGPNQMCFNTRGGYQCLDTPCPNSYQRGRNPG 4087          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Match: ltbp1 (latent transforming growth factor beta binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-091202-8])

HSP 1 Score: 135.961 bits (341), Expect = 1.943e-31
Identity = 104/267 (38.95%), Postives = 134/267 (50.19%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCY-CSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G +C DI+EC +     +  C NT GSF+C  C +  E  N   G    I EC  +R    +C      +   CS+  G   C C  GF  T D K  C DI+EC  +    +  C+N+ GSF C C  GF+ S     C D+DEC    + C G G C N++GSY+C C  GY  V    C+D+DEC+   +LC   N  C NT GSF CVC  G      GC D+DEC  N NTC    TC N+ G F C C+ GF  + D + C
Sbjct:  840 GSSCSDIDECQNRRACPSGLCYNTLGSFMCSPCPDGFEGKN---GQCVDINECLDKR----VC------AHGQCSNLEGYFVCTCDEGFLPTPDGKS-CTDIDECQDDNVCIQGHCQNTQGSFICNCEPGFKLSSSGDQCEDVDECLVIPQTCDGVGQCVNILGSYQCNCPQGYRQVNSTSCLDVDECEDDTQLCI-PNGECLNTEGSFLCVCDAGFVSTRTGCQDVDECSLNENTCGSHGTCQNTIGSFICQCDPGFKDSQDGQGC 1091          

HSP 2 Score: 118.242 bits (295), Expect = 4.859e-26
Identity = 92/264 (34.85%), Postives = 131/264 (49.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYL-NPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTC 3344
            C  G++     C+DINEC       + +C N  G FVC C  +E +L  P+      I+EC  +   I            +C ++ G   C C  GF      + C D++EC + P  C     C N  GS+ C CP+G++      C+D+DEC  D+++C  NG C N  GS+ C C  G+      GC D+DEC  ++  C G +  CQNT GSF C C  G +  +  +GCVD++EC+   N C E +TC N  G F C C
Sbjct:  872 CPDGFEGKNGQCVDINECLDKRVCAHGQCSNLEGYFVCTC--DEGFLPTPDGKSCTDIDECQDDNVCIQ----------GHCQNTQGSFICNCEPGFKLSSSGDQCEDVDECLVIPQTCDGVGQCVNILGSYQCNCPQGYRQVNSTSCLDVDECEDDTQLCIPNGECLNTEGSFLCVCDAGFVSTR-TGCQDVDECSLNENTC-GSHGTCQNTIGSFICQCDPGFKDSQDGQGCVDVNECELLGNVCGE-ATCENREGTFLCLC 1120          

HSP 3 Score: 107.457 bits (267), Expect = 9.230e-23
Identity = 86/251 (34.26%), Postives = 121/251 (48.21%), Query Frame = 3
Query: 2661 CINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            C NT  S+ C+C +     +    C   ++EC+   +  S+C      +   C ++ G   CVC  GF  + +   C DI+EC          C N+ GSF C  CP GF+   +  CVDI+EC D ++C+ +G C N+ G + C C  G+    D K C DIDECQ      QG    CQNT GSF C C  G +    G  C D+DEC     TC     C+N  G + C C +G+   N T   ++D+
Sbjct:  777 CYNTAESYTCFCDDGYRTDSEGTTCV-DVDECA---ENPSLC------ANGRCQNTPGGFLCVCELGFMPNERGSSCSDIDECQNRRACPSGLCYNTLGSFMCSPCPDGFE-GKNGQCVDINECLDKRVCA-HGQCSNLEGYFVCTCDEGFLPTPDGKSCTDIDECQDDNVCIQGH---CQNTQGSFICNCEPGFKLSSSGDQCEDVDECLVIPQTCDGVGQCVNILGSYQCNCPQGYRQVNSTSCLDVDE 1012          

HSP 4 Score: 107.071 bits (266), Expect = 1.200e-22
Identity = 73/217 (33.64%), Postives = 107/217 (49.31%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            G +C DI+EC          C NT GSF+C C        P F  +   ++C    + + I    TC     C +  G   C CP+G+       C+D++EC  +   C+    C N+ GSF C C  GF  S   GC D+DEC  +   C  +G CQN IGS+ C C  G++  +D +GC+D++EC+    +C      C+N  G+F C+CP  L+
Sbjct:  921 GKSCTDIDECQDDNVCIQGHCQNTQGSFICNCE-------PGFKLSSSGDQCEDVDECLVI--PQTCDGVGQCVNILGSYQCNCPQGYRQVNSTSCLDVDECEDDTQLCIPNGECLNTEGSFLCVCDAGF-VSTRTGCQDVDECSLNENTCGSHGTCQNTIGSFICQCDPGFKDSQDGQGCVDVNECELLGNVC--GEATCENREGTFLCLCPDDLQ 1125          

HSP 5 Score: 96.6709 bits (239), Expect = 2.012e-19
Identity = 68/168 (40.48%), Postives = 87/168 (51.79%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +I+EC + P +     C N+  S+ CFC  G++  S    CVD+DEC ++     NG CQN  G + C C  G+   E    C DIDECQ  +    G   +C NT GSF C  CP G E K   CVDI+EC     C  G  C N  G F CTC++GF+   D K C
Sbjct:  761 EIDECVVRPDICGAGICYNTAESYTCFCDDGYRTDSEGTTCVDVDECAENPSLCANGRCQNTPGGFLCVCELGFMPNERGSSCSDIDECQNRRACPSG---LCYNTLGSFMCSPCPDGFEGKNGQCVDINECLDKRVCAHGQ-CSNLEGYFVCTCDEGFLPTPDGKSC 924          

HSP 6 Score: 72.7886 bits (177), Expect = 4.053e-12
Identity = 51/144 (35.42%), Postives = 76/144 (52.78%), Query Frame = 3
Query: 2574 NCGKGYQKIGD-NCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQ 2987
            NC +GY+++   +C+D++EC  D+  C  N  C+NT GSF+C C  +  +++   GC   ++ECS            TC S   C ++ G   C C  GF      +GC+D+NEC +  N C  +ATC N  G+F C CP   Q
Sbjct:  994 NCPQGYRQVNSTSCLDVDECEDDTQLCIPNGECLNTEGSFLCVC--DAGFVSTRTGCQ-DVDECSLNEN--------TCGSHGTCQNTIGSFICQCDPGFKDSQDGQGCVDVNECELLGNVC-GEATCENREGTFLCLCPDDLQ 1125          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322])

HSP 1 Score: 136.346 bits (342), Expect = 2.887e-31
Identity = 98/287 (34.15%), Postives = 132/287 (45.99%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINECD-----SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV--CPRGF--TYDLKEGCIDINECN-MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCA--CHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG----CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+Q    G  C D NEC      S ACHN        G++ C C      ++ +      I+ECS    +        C    +C ++ G   CV  C RGF  T D    C D+NEC   NPC     C N+ GSF C C  G+Q   +  C+DI+EC+  ++C  +  C+N  G Y C   C  G     +  CIDIDEC      C+  N +C+NT GS+ C CP G  Y+ +G    C+DI+EC+     P    C+NS G + C C  G     D K C
Sbjct: 5067 CPSGFQLDPTGPYCSDENECVEGNPCSHACHN------AIGTYYCSCPRGLT-ISADGRTCQDIDECSLGGHM--------CHDGQDCENTIGSYRCVMRCGRGFRRTAD-GLSCSDVNECQESNPC--HQHCLNTIGSFRCACEPGYQLR-NRRCIDINECK-QRVCRSDQQCKNTRGGYTCIDLCPTGMTKGGNGTCIDIDECHDGTHQCRY-NQICENTRGSYHCTCPRG--YRSQGVGRPCLDINECELV-PVPCAYQCVNSPGSYKCLCPPGLHLLGDGKSC 5329          

HSP 2 Score: 92.8189 bits (229), Expect = 4.064e-18
Identity = 66/207 (31.88%), Postives = 93/207 (44.93%), Query Frame = 3
Query: 2577 CGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-----PKIEECSFERKI----------ISICDAVTCRS---------------PANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKI-CSGNGICQNMIGSYRC 3089
            C +GY  Q +G  C+DINEC+ +      +C+N+PGS+ C C      L     CA     P  E  S+  +           +    ++T +S               P +  S N     +CP GF    K G C+DINEC   + C  +  C N+ GS  C CP G++   +   C DIDEC +  I C  N +C NM GSY+C
Sbjct: 5275 CPRGYRSQGVGRPCLDINECELVPVPCAYQCVNSPGSYKCLCPPGLHLLGDGKSCAGLERLPSYETFSYGYRTSQSSSDRSSYLQRYHSLTSQSYHSYVPNGGRHVANRPNSAPSRNRRSTYICPHGFK--TKNGHCLDINECEQRDTC--QHECMNTPGSHRCLCPSGYRLMTNGKTCQDIDECLEQNIQCGANRMCFNMRGSYQC 5477          

HSP 3 Score: 67.0106 bits (162), Expect = 2.824e-10
Identity = 37/103 (35.92%), Postives = 53/103 (51.46%), Query Frame = 3
Query: 3093 CHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCT---CNKGFVFNNDTKFC 3383
            C +G++  ++  C+DI+EC+  +  CQ +   C NTPGS +C+CP G      G  C DIDEC +QN  C     C N  G + C    C   +  +  T FC
Sbjct: 5398 CPHGFK-TKNGHCLDINECE-QRDTCQHE---CMNTPGSHRCLCPSGYRLMTNGKTCQDIDECLEQNIQCGANRMCFNMRGSYQCIDTPCPPNYQRDPATGFC 5495          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Match: paqr8 (progestin and adipoQ receptor family member VIII [Source:Xenbase;Acc:XB-GENE-964788])

HSP 1 Score: 154.451 bits (389), Expect = 8.059e-37
Identity = 111/289 (38.41%), Postives = 158/289 (54.67%), Query Frame = 3
Query: 2577 CGKGY--QKIGDNCIDINEC-DSLACHNNSRCINTPGSFVC-YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG-LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLNVVQ 3413
            C  GY   + G +C+D+NEC    AC  +  CINT GSF C YC     + + + G    I EC   ++  S+C      +  +CS+  G   C C RG+  T D K+ CIDINEC          C+N+ GSF+C C +G+Q +     C DIDEC+++ +CS NG C+N  GS+ C CH GY+   D + C DIDECQ   ++C G    C NT GS++C CP G L      C DI+EC+Q + C     C+N+ G ++C C +GF  + + + CE +D+  N  Q
Sbjct:  905 CHAGYMANEQGIDCVDVNECLRPHACEEDGLCINTIGSFGCEYCEKG--FRHNQRGECEDINEC---QEDTSVC------ANGDCSNLEGSYRCTCHRGYDVTSDGKQ-CIDINECQSGTLCLNGLCQNTEGSFECSCNEGYQLAPSGDQCHDIDECQNTNLCS-NGQCRNTRGSFSCICHQGYKVSADGEQCEDIDECQEEAEVCLGGGS-CHNTDGSYECTCPDGFLLVDGIRCQDINECEQPDLCNPNGECLNTEGSYHCICEQGFTTSANGRSCEDVDECANTTQ 1179          

HSP 2 Score: 142.895 bits (359), Expect = 2.716e-33
Identity = 117/370 (31.62%), Postives = 175/370 (47.30%), Query Frame = 3
Query: 2358 FVVGRLTEMTTEDKEALLTEINLLKDEGHRLT---FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINEC-NMNPCLDKATCRNSYGSFDC-FCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFG 3437
             V  R  E T +D+  +  E   + + G         T+N D   P  + + S      L+  +S     +   V       +I +  +D+N C          CIN P  + C C    +    +  C   I EC+ +  + S            C ++ G   C+C  G+  + ++G  C+D+NEC   + C +   C N+ GSF C +C KGF+++    C DI+EC++      NG C N+ GSYRC CH GY+   D K CIDI+ECQ S  LC   N +CQNT GSF+C C  G +    G  C DIDECQ  N C  G  C N+ G F+C C++G+  + D + CE    ++  Q +A++  G
Sbjct:  757 HVPERKGEATDDDQAHVPFEERRVVEGGQPQVSPGISTVNLDGQFPVVVEKSSPPVPVALAPDVSTA---SASQVIAPTQVTEINECAVDVNIC------GEGNCINLPVGYTCLCFTGYKLNEQKTKCV-DINECAEDPHLCSH---------GRCENTKGRFLCICHAGYMAN-EQGIDCVDVNECLRPHACEEDGLCINTIGSFGCEYCEKGFRHNQRGECEDINECQEDTSVCANGDCSNLEGSYRCTCHRGYDVTSDGKQCIDINECQ-SGTLCL--NGLCQNTEGSFECSCNEGYQLAPSGDQCHDIDECQNTNLCSNGQ-CRNTRGSFSCICHQGYKVSADGEQCE---DIDECQEEAEVCLG 1099          

HSP 3 Score: 128.257 bits (321), Expect = 6.690e-29
Identity = 96/265 (36.23%), Postives = 130/265 (49.06%), Query Frame = 3
Query: 2577 CGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE--YKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTC 3344
            C +GY    D   CIDINEC S     N  C NT GSF C C  NE Y       AP  ++C      I  C      S   C ++ G   C+C +G+      E C DI+EC      CL   +C N+ GS++C CP GF       C DI+EC    +C+ NG C N  GSY C C  G+      + C D+DEC  + +   G   +C+NT GS++C+C  G +  +  +GCVD++EC+  +  C E + C N  G F C C
Sbjct:  989 CHRGYDVTSDGKQCIDINECQSGTLCLNGLCQNTEGSFECSC--NEGYQ-----LAPSGDQC----HDIDECQNTNLCSNGQCRNTRGSFSCICHQGYKVSADGEQCEDIDECQEEAEVCLGGGSCHNTDGSYECTCPDGFLLVDGIRCQDINECEQPDLCNPNGECLNTEGSYHCICEQGFTTSANGRSCEDVDECANTTQC--GSQGICENTDGSYRCLCYQGYQDSFDGQGCVDVNECEMLSGVCGE-ARCENVEGSFLCLC 1239          

HSP 4 Score: 77.0258 bits (188), Expect = 2.907e-13
Identity = 99/374 (26.47%), Postives = 154/374 (41.18%), Query Frame = 3
Query: 2601 GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFGCVDIDECR----------DSKICS----GNGICQNMIGS---------------------YRCA----------CHNGYEYVEDKGCI---------DIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAII 3536
            G  C DINEC+    C+ N  C+NT GS+ C C            C   ++EC+         +   C S   C +++G   C+C +G+  ++D  +GC+D+NEC M +    +A C N  GSF C C    Q Y    G      CR          D K C     G+  CQN++ +                     Y C           C  G  Y+  K  +         D DEC    K++C+  N  C NT  S++C C  G  Y   +  C+D +EC+  ++C +G  CIN+ G ++C C    + +   K C        +QPK      +DTSE  +   D+ +D     +++  + S  ++
Sbjct: 1123 GIRCQDINECEQPDLCNPNGECLNTEGSYHCICEQGFTTSANGRSCE-DVDECA---------NTTQCGSQGICENTDGSYRCLCYQGYQDSFD-GQGCVDVNECEMLSGVCGEARCENVEGSFLCLCTDESQEYDPMTG-----HCRNHNLHDENDEDQKDCYYNIIGDDFCQNVLAANVSKQECCCTLGAGWGDNCEIYPCPVIGSDEFTEMCPGGKGYIPSKESMYEGNDLSYQDADECMLFGKEICK--NGYCLNTESSYECYCKQGTYYDPVKLQCIDNNECEDPSSCIDGQ-CINNEGSYSCFCTHPMILDESGKRC--------IQPKP-----VDTSEPTEE-TDVYQDFCWQELSEEFVCSQPLV 1463          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Match: FBN3 (fibrillin 3 [Source:NCBI gene;Acc:100151721])

HSP 1 Score: 150.214 bits (378), Expect = 1.758e-35
Identity = 107/275 (38.91%), Postives = 151/275 (54.91%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C DI+EC S    C  N+ CIN PGS+ C CS+  + L+P   C  +  EC   ++I ++C      S   C  + G   CVC  GF     +  C+DI+EC+  PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N +GS++C C  GYE   D K C+DI+EC +    C      CQN  GSF+C+CP G E +   C+DI+EC ++ N C  G TC N+ G F C C  GFV +++ + C
Sbjct: 1776 GFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYK-LSPSGACVGR-NEC---QEIPNVC------SHGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQPC-GNGTCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLVGQVCR-NGQCINNVGSFQCLCQEGYEITPDGKNCMDINECTSLPGTCSPG--TCQNLEGSFRCICPPGFEVQNDNCIDINECDEEPNICLFG-TCTNTPGSFQCVCPPGFVLSDNGRRC 2034          

HSP 2 Score: 130.568 bits (327), Expect = 2.103e-29
Identity = 99/278 (35.61%), Postives = 136/278 (48.92%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+Q   D   C+DI+EC  +    ++ C N+ GS+ C CS     +  +  CA  I+EC        ICD         C++  G   C+C  GF   L  + CID+NEC++NP  CL    C N+ GSF C C  G F      GC DIDEC   +  C  +  C N+ GS++C C  G+   +   C+D+DEC      C   N  C NTPGS++C C  G       C D+DEC  N    E   C+N+ GG+ C C  GF    D+K C+
Sbjct: 1164 SCDSGFQSTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCSEGYALMPDQRSCA-DIDEC---EDNPDICDG------GQCTNIPGEYRCLCFDGFMASLDMKTCIDVNECDLNPNICL-HGECENTKGSFICHCQLGYFVKKGTTGCTDIDECEVGAHNCDVHASCLNVPGSFKCQCQTGW-VGDGLNCVDLDECTTEDHKCNA-NANCVNTPGSYRCACNEGFNGDGFICSDVDECADNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQ 1428          

HSP 3 Score: 120.553 bits (301), Expect = 2.098e-26
Identity = 95/264 (35.98%), Postives = 133/264 (50.38%), Query Frame = 3
Query: 2616 DINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D+NEC   +SL  H   R  N+ GSF C C +       E  C   I+EC     +      V      +C   +G E      GF   + + C+DI+EC  +P L +  TC N+ GSF+C CP G + +     CVDI+EC  S     NG C NMIG+Y+C+C +G++   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L   ++ C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1011 DVNECKVFNSLCTHGTCR--NSIGSFRCVCDSGFALDAEERNCT-DIDECRISPDLCGHGTCVNTPGSFDCECFDGYE-----SGFM--MMKNCMDIDECERDPLLCRGGTCINTDGSFECICPPGHELTSEGNACVDINECSLSDNLCRNGRCINMIGTYQCSCDSGFQSTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDQRSCADIDECEDNPDICDGGQCTNIPGEYRCLCFDGFMASLDMKTC 1261          

HSP 4 Score: 116.701 bits (291), Expect = 3.027e-25
Identity = 105/314 (33.44%), Postives = 147/314 (46.82%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   F T   D+D  + +  L         Q ++ +    C   +C  GY     G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++ECS ++                C ++ G   C CP GFT   +  CID NEC+  P  C  +  C+N+ GSF C C KGF   S    C D+DEC  +  C     CQN++G +RC C  GY ++ +   C+D +EC  S+  C   +  C NT GSFKCVCP G ++ +   GC D+DEC      P    C N+ GG+ C C  G+
Sbjct: 2376 HGPGFATDGRDIDECKVLPNLCKN-----GQCINTIGSFRC---HCKLGYTTDITGTTCVDLDECAQSPKPCNFICKNTEGSYQCSCPRG--YILQEDGKTCKDLDECSTKQHNCQFL----------CVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG--CQNVLGGFRCGCPQGYVQHYQWNQCVDENEC-TSQSTC--GSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGY 2663          

HSP 5 Score: 111.309 bits (277), Expect = 1.159e-23
Identity = 74/184 (40.22%), Postives = 101/184 (54.89%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D +  CID++EC+ +PC+    C N+ GS+ C C +G+Q +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC A+  +C   N +C N  GSFKC+C  G      G  CVD+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  457 CECNMGYKQDSRLECIDVDECSSSPCI-HGDCVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCRNGRCVNTEGSFQCICNAGFETTPDGKNCVDHDEC-ATTNMCL--NGMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGR-CINTEGSFRCECLGGLTIGIDGRVC 635          

HSP 6 Score: 110.538 bits (275), Expect = 2.451e-23
Identity = 104/300 (34.67%), Postives = 141/300 (47.00%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDA------VTCRSPANCSSSNG----VEFC----------VCPRG--FTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G Q+   G+ C+D NEC +      N RC+NT GS+ C C+   +  +    C    +   F   + ++C        +  +S   C+S  G     E C          +CP G  F  D +    DI+EC + P L K   C N+ GSF C C  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DEC   +  CQ    +C NT GSF C CP G    +  C+D +EC  Q + C     C N+ G F C C KGF  ++    CE
Sbjct: 2260 CPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTECVDTRQGFCFTEVLQTMCQLSSTNRNMVTKSECCCNSGRGWGNQCEICPLPGTGAFKKMCPHGPGFATDGR----DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCVDLDECAQSPKPC--NFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQ---FLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE 2550          

HSP 7 Score: 108.612 bits (270), Expect = 8.169e-23
Identity = 68/194 (35.05%), Postives = 105/194 (54.12%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +++G   C CP G+  D     C+D +EC++ NPC    TC N  G F+C C +GF+      C DI+EC +  +C+    C N  GSY C C +GY   ED+  C D+DEC      C+   ++C+N  G+F C+CP G++ +   +GC+D +EC+          C+N+ G + C CN+GF  ++    C
Sbjct: 2125 CINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC-GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLCAFR--CINTYGSYECTCPSGYTLREDRRMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTEC 2315          

HSP 8 Score: 108.612 bits (270), Expect = 8.449e-23
Identity = 93/277 (33.57%), Postives = 127/277 (45.85%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G G+   G    DI+EC  L     N +CINT GSF C+C            C   ++EC+   K            P N  C ++ G   C CPRG+   +  + C D++EC+      +  C N+ GSF+C CP GF       C+D +EC     +C   G+CQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G F+C CP G    Y+   CVD +EC   +TC   S C N+ G F C C  GF F+     C+
Sbjct: 2377 GPGFATDGR---DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCV-DLDECAQSPK------------PCNFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG----CQNVLGGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSAS-CYNTLGSFKCVCPSGFDFDQSFGGCQ 2631          

HSP 9 Score: 100.138 bits (248), Expect = 3.679e-20
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVV 3410
            GF  D++ G  +DI+EC+  P +     C N  GSF C CP GF Y+ +   C DIDEC   + +C  N  C N+ GSYRC C +GY+      C+  +ECQ    +C   +  C +T GS+ CVC  G +    +  C+DIDEC +   C  G TC N+ G +NC C  GF   ++    ++D+  ++V
Sbjct: 1734 GFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCS--HGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQ-PCGNG-TCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLV 1919          

HSP 10 Score: 98.2117 bits (243), Expect = 1.205e-19
Identity = 91/310 (29.35%), Postives = 134/310 (43.23%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDECQASKKLCQGDNVVCQNTPG-----------------------------SFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G NC+D++EC  +   C+ N+ C+NTPGS+ C C  NE +    F C+  ++EC+         D V      +C ++ G   C C  GFT  +  + C DI+EC         TC+N  G F C C  G++     G C D++EC D   C  NG+C N  G+Y C C   +E      GC+D  +  C    +      + C    G                              +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1333 CQTGWVGDGLNCVDLDECTTEDHKCNANANCVNTPGSYRCAC--NEGFNGDGFICS-DVDECA---------DNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQDIDECTFQNICVFGTCQNLPGMFRCICDDGYELDRSGGNCTDVNECADPVNCI-NGLCVNTPGNYLCNCPQDFELNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGRAWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1629          

HSP 11 Score: 97.8265 bits (242), Expect = 1.659e-19
Identity = 63/155 (40.65%), Postives = 79/155 (50.97%), Query Frame = 3
Query: 2928 KATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
               C  +  S+ C C  G++      C+D+DEC  S    G+  C N  GSY C CH GY+    K  CIDIDEC  +  +C+  N  C NT GSF+C+C  G E    G  CVD DEC   N C  G  CIN  G F C C  GFV   + ++C
Sbjct:  445 NGRCIPTPASYRCECNMGYKQDSRLECIDVDECSSSPCIHGD--CVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCR--NGRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLNG-MCINEDGSFKCICKPGFVLAPNGRYC 594          

HSP 12 Score: 89.3521 bits (220), Expect = 5.697e-17
Identity = 98/297 (33.00%), Postives = 129/297 (43.43%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CPK---------------GFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        C+NT GSF C C     YLN E      I+ECS     I IC       P  C ++ G   CVCP  +   +    C+D+              NE + N  + K  C  SY     +      CP                GF   +  G  +DIDEC +      NG+C N IGS+RC C  G+ Y      C DIDEC + + LCQ  N  C N PGS++C C  G +    G CV  +ECQ+  N C  G TC+++ G + C C+ GF  + D   C
Sbjct: 1588 DIDECQELPGLCQGGNCVNTFGSFQCECPAGY-YLNEETRICEDIDECSAH---IGIC------GPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSN--MTKKMCCCSYNIGKAWNKPCEPCPSPATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQ-RNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCSHG-TCMDTEGSYICVCHNGFKASGDQTMC 1870          

HSP 13 Score: 83.5741 bits (205), Expect = 3.230e-15
Identity = 98/303 (32.34%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DECQAS--KKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY++     CID++EC S  C +   C+NTPGS+ C C    +  NP       I+EC        I + V CR+   C ++ G   C+C  GF T    + C+D +EC   N CL+   C N  GSF C C  GF  + +   CVD+DEC+   IC  NG C N  GS+RC C  G    ++ + C+D      C  +  K  C                    G    CQ  P      F+ +C  G+     G  DI+EC  N+  CP G  C N  G + C CN G+  +   K C
Sbjct:  459 CNMGYKQDSRLECIDVDECSSSPCIHGD-CVNTPGSYHCRCHEGYQS-NPTKQACIDIDEC--------IMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICM-NGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG-RDINECALNSEICPNG-ICENLRGSYRCICNLGYESDASGKNC 746          

HSP 14 Score: 82.8037 bits (203), Expect = 6.715e-15
Identity = 90/308 (29.22%), Postives = 129/308 (41.88%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--------HF------------------GCVDIDECRDSKICSGNG-ICQ----NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C+D++EC          C N+ GSF C C  G    +        H                   G V   EC  +    G G  CQ         ++  C +G     D    DI+EC  + ++C   N +C+N  GS++C+C +G E    G  CVD+DEC  N    +   C N+ G + CTC KG+VF  D++ CE
Sbjct:  498 CHEGYQSNPTKQACIDIDECIMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDH-DECATTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG--RDINECALNSEICP--NGICENLRGSYRCICNLGYESDASGKNCVDVDECAVNRLLCDNGLCRNTPGSYTCTCPKGYVFKIDSETCE 789          

HSP 15 Score: 80.8777 bits (198), Expect = 2.055e-14
Identity = 105/373 (28.15%), Postives = 143/373 (38.34%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKI-------------ISICDAVTCRSPANCSSSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTPGSFKCVCPVGLEY---------------KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            Q+L  +F   C D   G    + G NC D+NEC       N  C+NTPG+++C C  + E LNP   GC   ++  C  E +              + +  A  C S    +  N  EFC          +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P       P   EY               +    +DIDEC +         CIN  G F C C  GF +NN    C    E     N+ Q  AD +
Sbjct: 1445 QNLPGMFRCICDD---GYELDRSGGNCTDVNECADPVNCINGLCVNTPGNYLCNCPQDFE-LNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGR-AWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECSAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSNMTKKMCCCSYNIGKAWNKPCEPCPS------PATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCI 1806          

HSP 16 Score: 80.8777 bits (198), Expect = 2.090e-14
Identity = 102/353 (28.90%), Postives = 135/353 (38.24%), Query Frame = 3
Query: 2457 ITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKI--GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINE-----------CNMNPCLDKATCR--------NSYG------------SFDCFCPKGFQYSVHFGCV--DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +T NND       Y L  +  +   Q ++ V    C    C +GY+    G NC+DINEC SL    +   C N  GSF C C    E  N        I EC  E  I              C+++ G   CVCP GF   D    C D  +           C++    +    R          +G            +F   CP G       G    D++EC +S     NG+C N  GS+RC C  GY        C+D DEC          N  C N  G F+C C  G E      C DI+EC     C     CIN++G + CTC  G+    D + C +LD+
Sbjct: 1903 LTHNNDCMDIDECYSLVGQVCRN-GQCINNVGSFQCL---CQEGYEITPDGKNCMDINECTSLPGTCSPGTCQNLEGSFRCICPPGFEVQNDN---CIDINECDEEPNICLF---------GTCTNTPGSFQCVCPPGFVLSDNGRRCFDTRQSFCFTRFDNGKCSVPKAFNTTKARCCCSKMPGEGWGDPCELCPQEGSVAFQELCPYGHGSIPGMGDTREDVNECSESPGICVNGVCINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC---GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLC--AFRCINTYGSYECTCPSGYTLREDRRMCKDLDE 2234          

HSP 17 Score: 78.5666 bits (192), Expect = 1.007e-13
Identity = 70/230 (30.43%), Postives = 93/230 (40.43%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK--------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   +T K+L +  +K        V  I   +  C  G+ +   +CID NEC S    C +   C N PGSF C C       +    C   ++EC    +    C  V            G   C CP+G+    +   C+D NEC        A+C N+ GSF C CP GF +   F GC D+DEC        +  C N  G Y C C  GY       C+
Sbjct: 2455 PRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE-DVDECDGNHRCQHGCQNVL-----------GGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGYFRAGQGHCV 2672          

HSP 18 Score: 65.4698 bits (158), Expect = 1.022e-9
Identity = 89/332 (26.81%), Postives = 132/332 (39.76%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC-IDIN----ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLC-----QGDNVVCQN--TPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+D++EC  + L C +N  C NTPGS+ C C     +      C   I EC+    +  +C            ++ G   C C  G   D         +K  C ++I     E N+N    K+ C  + G+           D  CP+G+       C D++EC        NG C N  GS++C C  G       + C+D+           DEC A         +C         + C+    PGS  FK +CP G  +  +G V        D++EC+  N+     TC NS G F C C+ GF  + + + C
Sbjct:  733 CNLGYESDASGKNCVDVDECAVNRLLC-DNGLCRNTPGSYTCTCPKGYVFKIDSETCE-DINECASNPCVNGVC-----------RNNAGSFVCECSFGSKLDSTGTICVDSMKGTCWLNIQDGRCEVNINGATLKSECCATLGAAWGSPCERCEIDPACPRGYSRKKGVACEDVNECEVFPGVCPNGRCVNTAGSFKCECPEGLTLDGSGRNCVDVRVEQCYMKWDEDECTAQLPGKYRMDMCCCSVGAAWGIDCEECPKPGSDEFKALCPRGPGFANRGDVLSGRPFYKDVNECKVFNSLCTHGTCRNSIGSFRCVCDSGFALDAEERNC 1051          

HSP 19 Score: 62.003 bits (149), Expect = 1.350e-8
Identity = 37/92 (40.22%), Postives = 48/92 (52.17%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +    C D+DECQA   LCQG    C NT GS++C CP G +  E  + C DIDEC       +G  C N+ G + C C +GF  + D   C
Sbjct:  224 IRTGACQDVDECQAIPGLCQGG--TCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGECTNTAGSYVCVCPRGFTTSRDGSRC 313          

HSP 20 Score: 61.6178 bits (148), Expect = 1.753e-8
Identity = 42/104 (40.38%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    +C G G C N +GSY C C  G++  E  + C DIDEC     +C G    C NT GS+ CVCP G      G   ID+
Sbjct:  216 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GTCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGE--CTNTAGSYVCVCPRGFTTSRDGSRCIDQ 316          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Match: FBN3 (fibrillin 3 [Source:NCBI gene;Acc:100151721])

HSP 1 Score: 150.214 bits (378), Expect = 2.013e-35
Identity = 107/275 (38.91%), Postives = 151/275 (54.91%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C DI+EC S    C  N+ CIN PGS+ C CS+  + L+P   C  +  EC   ++I ++C      S   C  + G   CVC  GF     +  C+DI+EC+  PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N +GS++C C  GYE   D K C+DI+EC +    C      CQN  GSF+C+CP G E +   C+DI+EC ++ N C  G TC N+ G F C C  GFV +++ + C
Sbjct: 1785 GFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYK-LSPSGACVGR-NEC---QEIPNVC------SHGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQPC-GNGTCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLVGQVCR-NGQCINNVGSFQCLCQEGYEITPDGKNCMDINECTSLPGTCSPG--TCQNLEGSFRCICPPGFEVQNDNCIDINECDEEPNICLFG-TCTNTPGSFQCVCPPGFVLSDNGRRC 2043          

HSP 2 Score: 130.183 bits (326), Expect = 2.195e-29
Identity = 99/278 (35.61%), Postives = 136/278 (48.92%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+Q   D   C+DI+EC  +    ++ C N+ GS+ C CS     +  +  CA  I+EC        ICD         C++  G   C+C  GF   L  + CID+NEC++NP  CL    C N+ GSF C C  G F      GC DIDEC   +  C  +  C N+ GS++C C  G+   +   C+D+DEC      C   N  C NTPGS++C C  G       C D+DEC  N    E   C+N+ GG+ C C  GF    D+K C+
Sbjct: 1173 SCDSGFQSTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCSEGYALMPDQRSCA-DIDEC---EDNPDICDG------GQCTNIPGEYRCLCFDGFMASLDMKTCIDVNECDLNPNICL-HGECENTKGSFICHCQLGYFVKKGTTGCTDIDECEVGAHNCDVHASCLNVPGSFKCQCQTGW-VGDGLNCVDLDECTTEDHKCNA-NANCVNTPGSYRCACNEGFNGDGFICSDVDECADNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQ 1437          

HSP 3 Score: 120.553 bits (301), Expect = 2.190e-26
Identity = 95/264 (35.98%), Postives = 133/264 (50.38%), Query Frame = 3
Query: 2616 DINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D+NEC   +SL  H   R  N+ GSF C C +       E  C   I+EC     +      V      +C   +G E      GF   + + C+DI+EC  +P L +  TC N+ GSF+C CP G + +     CVDI+EC  S     NG C NMIG+Y+C+C +G++   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L   ++ C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1020 DVNECKVFNSLCTHGTCR--NSIGSFRCVCDSGFALDAEERNCT-DIDECRISPDLCGHGTCVNTPGSFDCECFDGYE-----SGFM--MMKNCMDIDECERDPLLCRGGTCINTDGSFECICPPGHELTSEGNACVDINECSLSDNLCRNGRCINMIGTYQCSCDSGFQSTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDQRSCADIDECEDNPDICDGGQCTNIPGEYRCLCFDGFMASLDMKTC 1270          

HSP 4 Score: 116.701 bits (291), Expect = 2.761e-25
Identity = 105/314 (33.44%), Postives = 147/314 (46.82%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   F T   D+D  + +  L         Q ++ +    C   +C  GY     G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++ECS ++                C ++ G   C CP GFT   +  CID NEC+  P  C  +  C+N+ GSF C C KGF   S    C D+DEC  +  C     CQN++G +RC C  GY ++ +   C+D +EC  S+  C   +  C NT GSFKCVCP G ++ +   GC D+DEC      P    C N+ GG+ C C  G+
Sbjct: 2384 HGPGFATDGRDIDECKVLPNLCKN-----GQCINTIGSFRC---HCKLGYTTDITGTTCVDLDECAQSPKPCNFICKNTEGSYQCSCPRG--YILQEDGKTCKDLDECSTKQHNCQFL----------CVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG--CQNVLGGFRCGCPQGYVQHYQWNQCVDENEC-TSQSTC--GSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGY 2671          

HSP 5 Score: 111.309 bits (277), Expect = 1.251e-23
Identity = 74/184 (40.22%), Postives = 101/184 (54.89%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D +  CID++EC+ +PC+    C N+ GS+ C C +G+Q +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC A+  +C   N +C N  GSFKC+C  G      G  CVD+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  466 CECNMGYKQDSRLECIDVDECSSSPCI-HGDCVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCRNGRCVNTEGSFQCICNAGFETTPDGKNCVDHDEC-ATTNMCL--NGMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGR-CINTEGSFRCECLGGLTIGIDGRVC 644          

HSP 6 Score: 110.153 bits (274), Expect = 2.691e-23
Identity = 104/300 (34.67%), Postives = 141/300 (47.00%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDA------VTCRSPANCSSSNG----VEFC----------VCPRG--FTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G Q+   G+ C+D NEC +      N RC+NT GS+ C C+   +  +    C    +   F   + ++C        +  +S   C+S  G     E C          +CP G  F  D +    DI+EC + P L K   C N+ GSF C C  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DEC   +  CQ    +C NT GSF C CP G    +  C+D +EC  Q + C     C N+ G F C C KGF  ++    CE
Sbjct: 2268 CPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTECVDTRQGFCFTEVLQTMCQLSSTNRNMVTKSECCCNSGRGWGNQCEICPLPGTGAFKKMCPHGPGFATDGR----DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCVDLDECAQSPKPC--NFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQ---FLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE 2558          

HSP 7 Score: 108.612 bits (270), Expect = 8.670e-23
Identity = 93/277 (33.57%), Postives = 127/277 (45.85%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G G+   G    DI+EC  L     N +CINT GSF C+C            C   ++EC+   K            P N  C ++ G   C CPRG+   +  + C D++EC+      +  C N+ GSF+C CP GF       C+D +EC     +C   G+CQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G F+C CP G    Y+   CVD +EC   +TC   S C N+ G F C C  GF F+     C+
Sbjct: 2385 GPGFATDGR---DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCV-DLDECAQSPK------------PCNFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG----CQNVLGGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSAS-CYNTLGSFKCVCPSGFDFDQSFGGCQ 2639          

HSP 8 Score: 108.612 bits (270), Expect = 8.966e-23
Identity = 68/194 (35.05%), Postives = 105/194 (54.12%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +++G   C CP G+  D     C+D +EC++ NPC    TC N  G F+C C +GF+      C DI+EC +  +C+    C N  GSY C C +GY   ED+  C D+DEC      C+   ++C+N  G+F C+CP G++ +   +GC+D +EC+          C+N+ G + C CN+GF  ++    C
Sbjct: 2133 CINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC-GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLCAFR--CINTYGSYECTCPSGYTLREDRRMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTEC 2323          

HSP 9 Score: 99.7525 bits (247), Expect = 4.211e-20
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVV 3410
            GF  D++ G  +DI+EC+  P +     C N  GSF C CP GF Y+ +   C DIDEC   + +C  N  C N+ GSYRC C +GY+      C+  +ECQ    +C   +  C +T GS+ CVC  G +    +  C+DIDEC +   C  G TC N+ G +NC C  GF   ++    ++D+  ++V
Sbjct: 1743 GFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCS--HGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQ-PCGNG-TCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLV 1928          

HSP 10 Score: 98.2117 bits (243), Expect = 1.391e-19
Identity = 91/310 (29.35%), Postives = 134/310 (43.23%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDECQASKKLCQGDNVVCQNTPG-----------------------------SFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G NC+D++EC  +   C+ N+ C+NTPGS+ C C  NE +    F C+  ++EC+         D V      +C ++ G   C C  GFT  +  + C DI+EC         TC+N  G F C C  G++     G C D++EC D   C  NG+C N  G+Y C C   +E      GC+D  +  C    +      + C    G                              +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1342 CQTGWVGDGLNCVDLDECTTEDHKCNANANCVNTPGSYRCAC--NEGFNGDGFICS-DVDECA---------DNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQDIDECTFQNICVFGTCQNLPGMFRCICDDGYELDRSGGNCTDVNECADPVNCI-NGLCVNTPGNYLCNCPQDFELNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGRAWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1638          

HSP 11 Score: 97.8265 bits (242), Expect = 1.867e-19
Identity = 63/155 (40.65%), Postives = 79/155 (50.97%), Query Frame = 3
Query: 2928 KATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
               C  +  S+ C C  G++      C+D+DEC  S    G+  C N  GSY C CH GY+    K  CIDIDEC  +  +C+  N  C NT GSF+C+C  G E    G  CVD DEC   N C  G  CIN  G F C C  GFV   + ++C
Sbjct:  454 NGRCIPTPASYRCECNMGYKQDSRLECIDVDECSSSPCIHGD--CVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCR--NGRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLNG-MCINEDGSFKCICKPGFVLAPNGRYC 603          

HSP 12 Score: 89.3521 bits (220), Expect = 5.995e-17
Identity = 98/297 (33.00%), Postives = 129/297 (43.43%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CPK---------------GFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        C+NT GSF C C     YLN E      I+ECS     I IC       P  C ++ G   CVCP  +   +    C+D+              NE + N  + K  C  SY     +      CP                GF   +  G  +DIDEC +      NG+C N IGS+RC C  G+ Y      C DIDEC + + LCQ  N  C N PGS++C C  G +    G CV  +ECQ+  N C  G TC+++ G + C C+ GF  + D   C
Sbjct: 1597 DIDECQELPGLCQGGNCVNTFGSFQCECPAGY-YLNEETRICEDIDECSAH---IGIC------GPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSN--MTKKMCCCSYNIGKAWNKPCEPCPSPATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQ-RNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCSHG-TCMDTEGSYICVCHNGFKASGDQTMC 1879          

HSP 13 Score: 83.5741 bits (205), Expect = 3.545e-15
Identity = 98/303 (32.34%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DECQAS--KKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY++     CID++EC S  C +   C+NTPGS+ C C    +  NP       I+EC        I + V CR+   C ++ G   C+C  GF T    + C+D +EC   N CL+   C N  GSF C C  GF  + +   CVD+DEC+   IC  NG C N  GS+RC C  G    ++ + C+D      C  +  K  C                    G    CQ  P      F+ +C  G+     G  DI+EC  N+  CP G  C N  G + C CN G+  +   K C
Sbjct:  468 CNMGYKQDSRLECIDVDECSSSPCIHGD-CVNTPGSYHCRCHEGYQS-NPTKQACIDIDEC--------IMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICM-NGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG-RDINECALNSEICPNG-ICENLRGSYRCICNLGYESDASGKNC 755          

HSP 14 Score: 82.4185 bits (202), Expect = 7.369e-15
Identity = 90/308 (29.22%), Postives = 129/308 (41.88%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--------HF------------------GCVDIDECRDSKICSGNG-ICQ----NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C+D++EC          C N+ GSF C C  G    +        H                   G V   EC  +    G G  CQ         ++  C +G     D    DI+EC  + ++C   N +C+N  GS++C+C +G E    G  CVD+DEC  N    +   C N+ G + CTC KG+VF  D++ CE
Sbjct:  507 CHEGYQSNPTKQACIDIDECIMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDH-DECATTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG--RDINECALNSEICP--NGICENLRGSYRCICNLGYESDASGKNCVDVDECAVNRLLCDNGLCRNTPGSYTCTCPKGYVFKIDSETCE 798          

HSP 15 Score: 81.2629 bits (199), Expect = 1.598e-14
Identity = 102/352 (28.98%), Postives = 135/352 (38.35%), Query Frame = 3
Query: 2457 ITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKI--GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINE-----------CNMNPCLDKATCR--------NSYG-----------SFDCFCPKGFQYSVHFGCV--DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +T NND       Y L  +  +   Q ++ V    C    C +GY+    G NC+DINEC SL    +   C N  GSF C C    E  N        I EC  E  I              C+++ G   CVCP GF   D    C D  +           C++    +    R          +G           +F   CP G       G    D++EC +S     NG+C N  GS+RC C  GY        C+D DEC          N  C N  G F+C C  G E      C DI+EC     C     CIN++G + CTC  G+    D + C +LD+
Sbjct: 1912 LTHNNDCMDIDECYSLVGQVCRN-GQCINNVGSFQCL---CQEGYEITPDGKNCMDINECTSLPGTCSPGTCQNLEGSFRCICPPGFEVQNDN---CIDINECDEEPNICLF---------GTCTNTPGSFQCVCPPGFVLSDNGRRCFDTRQSFCFTRFDNGKCSVPKAFNTTKARCCCSKMPGEGWGDPCELCPQEGIAFQELCPYGHGSIPGMGDTREDVNECSESPGICVNGVCINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC---GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLC--AFRCINTYGSYECTCPSGYTLREDRRMCKDLDE 2242          

HSP 16 Score: 80.8777 bits (198), Expect = 2.516e-14
Identity = 105/373 (28.15%), Postives = 143/373 (38.34%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKI-------------ISICDAVTCRSPANCSSSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTPGSFKCVCPVGLEY---------------KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            Q+L  +F   C D   G    + G NC D+NEC       N  C+NTPG+++C C  + E LNP   GC   ++  C  E +              + +  A  C S    +  N  EFC          +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P       P   EY               +    +DIDEC +         CIN  G F C C  GF +NN    C    E     N+ Q  AD +
Sbjct: 1454 QNLPGMFRCICDD---GYELDRSGGNCTDVNECADPVNCINGLCVNTPGNYLCNCPQDFE-LNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGR-AWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECSAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSNMTKKMCCCSYNIGKAWNKPCEPCPS------PATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCI 1815          

HSP 17 Score: 78.9518 bits (193), Expect = 8.809e-14
Identity = 70/230 (30.43%), Postives = 93/230 (40.43%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK--------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   +T K+L +  +K        V  I   +  C  G+ +   +CID NEC S    C +   C N PGSF C C       +    C   ++EC    +    C  V            G   C CP+G+    +   C+D NEC        A+C N+ GSF C CP GF +   F GC D+DEC        +  C N  G Y C C  GY       C+
Sbjct: 2463 PRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE-DVDECDGNHRCQHGCQNVL-----------GGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGYFRAGQGHCV 2680          

HSP 18 Score: 65.4698 bits (158), Expect = 1.103e-9
Identity = 89/332 (26.81%), Postives = 132/332 (39.76%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC-IDIN----ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLC-----QGDNVVCQN--TPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+D++EC  + L C +N  C NTPGS+ C C     +      C   I EC+    +  +C            ++ G   C C  G   D         +K  C ++I     E N+N    K+ C  + G+           D  CP+G+       C D++EC        NG C N  GS++C C  G       + C+D+           DEC A         +C         + C+    PGS  FK +CP G  +  +G V        D++EC+  N+     TC NS G F C C+ GF  + + + C
Sbjct:  742 CNLGYESDASGKNCVDVDECAVNRLLC-DNGLCRNTPGSYTCTCPKGYVFKIDSETCE-DINECASNPCVNGVC-----------RNNAGSFVCECSFGSKLDSTGTICVDSMKGTCWLNIQDGRCEVNINGATLKSECCATLGAAWGSPCERCEIDPACPRGYSRKKGVACEDVNECEVFPGVCPNGRCVNTAGSFKCECPEGLTLDGSGRNCVDVRVEQCYMKWDEDECTAQLPGKYRMDMCCCSVGAAWGIDCEECPKPGSDEFKALCPRGPGFANRGDVLSGRPFYKDVNECKVFNSLCTHGTCRNSIGSFRCVCDSGFALDAEERNC 1060          

HSP 19 Score: 61.6178 bits (148), Expect = 1.397e-8
Identity = 37/92 (40.22%), Postives = 48/92 (52.17%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +    C D+DECQA   LCQG    C NT GS++C CP G +  E  + C DIDEC       +G  C N+ G + C C +GF  + D   C
Sbjct:  233 IRTGACQDVDECQAIPGLCQGG--TCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGECTNTAGSYVCVCPRGFTTSRDGSRC 322          

HSP 20 Score: 61.2326 bits (147), Expect = 1.972e-8
Identity = 42/104 (40.38%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    +C G G C N +GSY C C  G++  E  + C DIDEC     +C G    C NT GS+ CVCP G      G   ID+
Sbjct:  225 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GTCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGE--CTNTAGSYVCVCPRGFTTSRDGSRCIDQ 325          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Match: FBN3 (fibrillin 3 [Source:NCBI gene;Acc:100151721])

HSP 1 Score: 150.214 bits (378), Expect = 2.050e-35
Identity = 107/275 (38.91%), Postives = 151/275 (54.91%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C DI+EC S    C  N+ CIN PGS+ C CS+  + L+P   C  +  EC   ++I ++C      S   C  + G   CVC  GF     +  C+DI+EC+  PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N +GS++C C  GYE   D K C+DI+EC +    C      CQN  GSF+C+CP G E +   C+DI+EC ++ N C  G TC N+ G F C C  GFV +++ + C
Sbjct: 1794 GFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYK-LSPSGACVGR-NEC---QEIPNVC------SHGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQPC-GNGTCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLVGQVCR-NGQCINNVGSFQCLCQEGYEITPDGKNCMDINECTSLPGTCSPG--TCQNLEGSFRCICPPGFEVQNDNCIDINECDEEPNICLFG-TCTNTPGSFQCVCPPGFVLSDNGRRC 2052          

HSP 2 Score: 130.183 bits (326), Expect = 2.331e-29
Identity = 99/278 (35.61%), Postives = 136/278 (48.92%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+Q   D   C+DI+EC  +    ++ C N+ GS+ C CS     +  +  CA  I+EC        ICD         C++  G   C+C  GF   L  + CID+NEC++NP  CL    C N+ GSF C C  G F      GC DIDEC   +  C  +  C N+ GS++C C  G+   +   C+D+DEC      C   N  C NTPGS++C C  G       C D+DEC  N    E   C+N+ GG+ C C  GF    D+K C+
Sbjct: 1182 SCDSGFQSTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCSEGYALMPDQRSCA-DIDEC---EDNPDICDG------GQCTNIPGEYRCLCFDGFMASLDMKTCIDVNECDLNPNICL-HGECENTKGSFICHCQLGYFVKKGTTGCTDIDECEVGAHNCDVHASCLNVPGSFKCQCQTGW-VGDGLNCVDLDECTTEDHKCNA-NANCVNTPGSYRCACNEGFNGDGFICSDVDECADNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQ 1446          

HSP 3 Score: 120.553 bits (301), Expect = 2.325e-26
Identity = 95/264 (35.98%), Postives = 133/264 (50.38%), Query Frame = 3
Query: 2616 DINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D+NEC   +SL  H   R  N+ GSF C C +       E  C   I+EC     +      V      +C   +G E      GF   + + C+DI+EC  +P L +  TC N+ GSF+C CP G + +     CVDI+EC  S     NG C NMIG+Y+C+C +G++   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L   ++ C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1029 DVNECKVFNSLCTHGTCR--NSIGSFRCVCDSGFALDAEERNCT-DIDECRISPDLCGHGTCVNTPGSFDCECFDGYE-----SGFM--MMKNCMDIDECERDPLLCRGGTCINTDGSFECICPPGHELTSEGNACVDINECSLSDNLCRNGRCINMIGTYQCSCDSGFQSTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDQRSCADIDECEDNPDICDGGQCTNIPGEYRCLCFDGFMASLDMKTC 1279          

HSP 4 Score: 116.701 bits (291), Expect = 3.135e-25
Identity = 105/314 (33.44%), Postives = 147/314 (46.82%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   F T   D+D  + +  L         Q ++ +    C   +C  GY     G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++ECS ++                C ++ G   C CP GFT   +  CID NEC+  P  C  +  C+N+ GSF C C KGF   S    C D+DEC  +  C     CQN++G +RC C  GY ++ +   C+D +EC  S+  C   +  C NT GSFKCVCP G ++ +   GC D+DEC      P    C N+ GG+ C C  G+
Sbjct: 2394 HGPGFATDGRDIDECKVLPNLCKN-----GQCINTIGSFRC---HCKLGYTTDITGTTCVDLDECAQSPKPCNFICKNTEGSYQCSCPRG--YILQEDGKTCKDLDECSTKQHNCQFL----------CVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG--CQNVLGGFRCGCPQGYVQHYQWNQCVDENEC-TSQSTC--GSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGY 2681          

HSP 5 Score: 111.309 bits (277), Expect = 1.221e-23
Identity = 74/184 (40.22%), Postives = 101/184 (54.89%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D +  CID++EC+ +PC+    C N+ GS+ C C +G+Q +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC A+  +C   N +C N  GSFKC+C  G      G  CVD+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  475 CECNMGYKQDSRLECIDVDECSSSPCI-HGDCVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCRNGRCVNTEGSFQCICNAGFETTPDGKNCVDHDEC-ATTNMCL--NGMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGR-CINTEGSFRCECLGGLTIGIDGRVC 653          

HSP 6 Score: 110.153 bits (274), Expect = 2.880e-23
Identity = 104/300 (34.67%), Postives = 141/300 (47.00%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDA------VTCRSPANCSSSNG----VEFC----------VCPRG--FTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G Q+   G+ C+D NEC +      N RC+NT GS+ C C+   +  +    C    +   F   + ++C        +  +S   C+S  G     E C          +CP G  F  D +    DI+EC + P L K   C N+ GSF C C  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DEC   +  CQ    +C NT GSF C CP G    +  C+D +EC  Q + C     C N+ G F C C KGF  ++    CE
Sbjct: 2278 CPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTECVDTRQGFCFTEVLQTMCQLSSTNRNMVTKSECCCNSGRGWGNQCEICPLPGTGAFKKMCPHGPGFATDGR----DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCVDLDECAQSPKPC--NFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQ---FLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE 2568          

HSP 7 Score: 108.612 bits (270), Expect = 8.897e-23
Identity = 68/194 (35.05%), Postives = 105/194 (54.12%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +++G   C CP G+  D     C+D +EC++ NPC    TC N  G F+C C +GF+      C DI+EC +  +C+    C N  GSY C C +GY   ED+  C D+DEC      C+   ++C+N  G+F C+CP G++ +   +GC+D +EC+          C+N+ G + C CN+GF  ++    C
Sbjct: 2143 CINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC-GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLCAFR--CINTYGSYECTCPSGYTLREDRRMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTEC 2333          

HSP 8 Score: 108.612 bits (270), Expect = 9.926e-23
Identity = 93/277 (33.57%), Postives = 127/277 (45.85%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G G+   G    DI+EC  L     N +CINT GSF C+C            C   ++EC+   K            P N  C ++ G   C CPRG+   +  + C D++EC+      +  C N+ GSF+C CP GF       C+D +EC     +C   G+CQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G F+C CP G    Y+   CVD +EC   +TC   S C N+ G F C C  GF F+     C+
Sbjct: 2395 GPGFATDGR---DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCV-DLDECAQSPK------------PCNFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG----CQNVLGGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSAS-CYNTLGSFKCVCPSGFDFDQSFGGCQ 2649          

HSP 9 Score: 99.7525 bits (247), Expect = 4.249e-20
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVV 3410
            GF  D++ G  +DI+EC+  P +     C N  GSF C CP GF Y+ +   C DIDEC   + +C  N  C N+ GSYRC C +GY+      C+  +ECQ    +C   +  C +T GS+ CVC  G +    +  C+DIDEC +   C  G TC N+ G +NC C  GF   ++    ++D+  ++V
Sbjct: 1752 GFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCS--HGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQ-PCGNG-TCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLV 1937          

HSP 10 Score: 98.2117 bits (243), Expect = 1.415e-19
Identity = 91/310 (29.35%), Postives = 134/310 (43.23%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDECQASKKLCQGDNVVCQNTPG-----------------------------SFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G NC+D++EC  +   C+ N+ C+NTPGS+ C C  NE +    F C+  ++EC+         D V      +C ++ G   C C  GFT  +  + C DI+EC         TC+N  G F C C  G++     G C D++EC D   C  NG+C N  G+Y C C   +E      GC+D  +  C    +      + C    G                              +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1351 CQTGWVGDGLNCVDLDECTTEDHKCNANANCVNTPGSYRCAC--NEGFNGDGFICS-DVDECA---------DNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQDIDECTFQNICVFGTCQNLPGMFRCICDDGYELDRSGGNCTDVNECADPVNCI-NGLCVNTPGNYLCNCPQDFELNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGRAWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1647          

HSP 11 Score: 97.4413 bits (241), Expect = 1.884e-19
Identity = 63/155 (40.65%), Postives = 79/155 (50.97%), Query Frame = 3
Query: 2928 KATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
               C  +  S+ C C  G++      C+D+DEC  S    G+  C N  GSY C CH GY+    K  CIDIDEC  +  +C+  N  C NT GSF+C+C  G E    G  CVD DEC   N C  G  CIN  G F C C  GFV   + ++C
Sbjct:  463 NGRCIPTPASYRCECNMGYKQDSRLECIDVDECSSSPCIHGD--CVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCR--NGRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLNG-MCINEDGSFKCICKPGFVLAPNGRYC 612          

HSP 12 Score: 89.3521 bits (220), Expect = 6.049e-17
Identity = 98/297 (33.00%), Postives = 129/297 (43.43%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CPK---------------GFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        C+NT GSF C C     YLN E      I+ECS     I IC       P  C ++ G   CVCP  +   +    C+D+              NE + N  + K  C  SY     +      CP                GF   +  G  +DIDEC +      NG+C N IGS+RC C  G+ Y      C DIDEC + + LCQ  N  C N PGS++C C  G +    G CV  +ECQ+  N C  G TC+++ G + C C+ GF  + D   C
Sbjct: 1606 DIDECQELPGLCQGGNCVNTFGSFQCECPAGY-YLNEETRICEDIDECSAH---IGIC------GPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSN--MTKKMCCCSYNIGKAWNKPCEPCPSPATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQ-RNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCSHG-TCMDTEGSYICVCHNGFKASGDQTMC 1888          

HSP 13 Score: 83.5741 bits (205), Expect = 3.400e-15
Identity = 98/303 (32.34%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DECQAS--KKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY++     CID++EC S  C +   C+NTPGS+ C C    +  NP       I+EC        I + V CR+   C ++ G   C+C  GF T    + C+D +EC   N CL+   C N  GSF C C  GF  + +   CVD+DEC+   IC  NG C N  GS+RC C  G    ++ + C+D      C  +  K  C                    G    CQ  P      F+ +C  G+     G  DI+EC  N+  CP G  C N  G + C CN G+  +   K C
Sbjct:  477 CNMGYKQDSRLECIDVDECSSSPCIHGD-CVNTPGSYHCRCHEGYQS-NPTKQACIDIDEC--------IMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICM-NGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG-RDINECALNSEICPNG-ICENLRGSYRCICNLGYESDASGKNC 764          

HSP 14 Score: 82.4185 bits (202), Expect = 7.435e-15
Identity = 90/308 (29.22%), Postives = 129/308 (41.88%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--------HF------------------GCVDIDECRDSKICSGNG-ICQ----NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C+D++EC          C N+ GSF C C  G    +        H                   G V   EC  +    G G  CQ         ++  C +G     D    DI+EC  + ++C   N +C+N  GS++C+C +G E    G  CVD+DEC  N    +   C N+ G + CTC KG+VF  D++ CE
Sbjct:  516 CHEGYQSNPTKQACIDIDECIMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDH-DECATTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG--RDINECALNSEICP--NGICENLRGSYRCICNLGYESDASGKNCVDVDECAVNRLLCDNGLCRNTPGSYTCTCPKGYVFKIDSETCE 807          

HSP 15 Score: 80.8777 bits (198), Expect = 2.200e-14
Identity = 102/353 (28.90%), Postives = 135/353 (38.24%), Query Frame = 3
Query: 2457 ITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKI--GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINE-----------CNMNPCLDKATCR--------NSYG------------SFDCFCPKGFQYSVHFGCV--DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +T NND       Y L  +  +   Q ++ V    C    C +GY+    G NC+DINEC SL    +   C N  GSF C C    E  N        I EC  E  I              C+++ G   CVCP GF   D    C D  +           C++    +    R          +G            +F   CP G       G    D++EC +S     NG+C N  GS+RC C  GY        C+D DEC          N  C N  G F+C C  G E      C DI+EC     C     CIN++G + CTC  G+    D + C +LD+
Sbjct: 1921 LTHNNDCMDIDECYSLVGQVCRN-GQCINNVGSFQCL---CQEGYEITPDGKNCMDINECTSLPGTCSPGTCQNLEGSFRCICPPGFEVQNDN---CIDINECDEEPNICLF---------GTCTNTPGSFQCVCPPGFVLSDNGRRCFDTRQSFCFTRFDNGKCSVPKAFNTTKARCCCSKMPGEGWGDPCELCPQEGSVAFQELCPYGHGSIPGMGDTREDVNECSESPGICVNGVCINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC---GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLC--AFRCINTYGSYECTCPSGYTLREDRRMCKDLDE 2252          

HSP 16 Score: 80.8777 bits (198), Expect = 2.581e-14
Identity = 105/373 (28.15%), Postives = 143/373 (38.34%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKI-------------ISICDAVTCRSPANCSSSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTPGSFKCVCPVGLEY---------------KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            Q+L  +F   C D   G    + G NC D+NEC       N  C+NTPG+++C C  + E LNP   GC   ++  C  E +              + +  A  C S    +  N  EFC          +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P       P   EY               +    +DIDEC +         CIN  G F C C  GF +NN    C    E     N+ Q  AD +
Sbjct: 1463 QNLPGMFRCICDD---GYELDRSGGNCTDVNECADPVNCINGLCVNTPGNYLCNCPQDFE-LNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGR-AWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECSAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSNMTKKMCCCSYNIGKAWNKPCEPCPS------PATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCI 1824          

HSP 17 Score: 78.5666 bits (192), Expect = 1.008e-13
Identity = 70/230 (30.43%), Postives = 93/230 (40.43%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK--------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   +T K+L +  +K        V  I   +  C  G+ +   +CID NEC S    C +   C N PGSF C C       +    C   ++EC    +    C  V            G   C CP+G+    +   C+D NEC        A+C N+ GSF C CP GF +   F GC D+DEC        +  C N  G Y C C  GY       C+
Sbjct: 2473 PRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE-DVDECDGNHRCQHGCQNVL-----------GGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGYFRAGQGHCV 2690          

HSP 18 Score: 65.4698 bits (158), Expect = 1.160e-9
Identity = 89/332 (26.81%), Postives = 132/332 (39.76%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC-IDIN----ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLC-----QGDNVVCQN--TPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+D++EC  + L C +N  C NTPGS+ C C     +      C   I EC+    +  +C            ++ G   C C  G   D         +K  C ++I     E N+N    K+ C  + G+           D  CP+G+       C D++EC        NG C N  GS++C C  G       + C+D+           DEC A         +C         + C+    PGS  FK +CP G  +  +G V        D++EC+  N+     TC NS G F C C+ GF  + + + C
Sbjct:  751 CNLGYESDASGKNCVDVDECAVNRLLC-DNGLCRNTPGSYTCTCPKGYVFKIDSETCE-DINECASNPCVNGVC-----------RNNAGSFVCECSFGSKLDSTGTICVDSMKGTCWLNIQDGRCEVNINGATLKSECCATLGAAWGSPCERCEIDPACPRGYSRKKGVACEDVNECEVFPGVCPNGRCVNTAGSFKCECPEGLTLDGSGRNCVDVRVEQCYMKWDEDECTAQLPGKYRMDMCCCSVGAAWGIDCEECPKPGSDEFKALCPRGPGFANRGDVLSGRPFYKDVNECKVFNSLCTHGTCRNSIGSFRCVCDSGFALDAEERNC 1069          

HSP 19 Score: 61.6178 bits (148), Expect = 1.397e-8
Identity = 37/92 (40.22%), Postives = 48/92 (52.17%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +    C D+DECQA   LCQG    C NT GS++C CP G +  E  + C DIDEC       +G  C N+ G + C C +GF  + D   C
Sbjct:  242 IRTGACQDVDECQAIPGLCQGG--TCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGECTNTAGSYVCVCPRGFTTSRDGSRC 331          

HSP 20 Score: 61.2326 bits (147), Expect = 1.956e-8
Identity = 42/104 (40.38%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    +C G G C N +GSY C C  G++  E  + C DIDEC     +C G    C NT GS+ CVCP G      G   ID+
Sbjct:  234 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GTCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGE--CTNTAGSYVCVCPRGFTTSRDGSRCIDQ 334          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Match: FBN3 (fibrillin 3 [Source:NCBI gene;Acc:100151721])

HSP 1 Score: 150.214 bits (378), Expect = 2.241e-35
Identity = 107/275 (38.91%), Postives = 151/275 (54.91%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C DI+EC S    C  N+ CIN PGS+ C CS+  + L+P   C  +  EC   ++I ++C      S   C  + G   CVC  GF     +  C+DI+EC+  PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N +GS++C C  GYE   D K C+DI+EC +    C      CQN  GSF+C+CP G E +   C+DI+EC ++ N C  G TC N+ G F C C  GFV +++ + C
Sbjct: 1770 GFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYK-LSPSGACVGR-NEC---QEIPNVC------SHGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQPC-GNGTCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLVGQVCR-NGQCINNVGSFQCLCQEGYEITPDGKNCMDINECTSLPGTCSPG--TCQNLEGSFRCICPPGFEVQNDNCIDINECDEEPNICLFG-TCTNTPGSFQCVCPPGFVLSDNGRRC 2028          

HSP 2 Score: 129.798 bits (325), Expect = 2.821e-29
Identity = 99/278 (35.61%), Postives = 136/278 (48.92%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  G+Q   D   C+DI+EC  +    ++ C N+ GS+ C CS     +  +  CA  I+EC        ICD         C++  G   C+C  GF   L  + CID+NEC++NP  CL    C N+ GSF C C  G F      GC DIDEC   +  C  +  C N+ GS++C C  G+   +   C+D+DEC      C   N  C NTPGS++C C  G       C D+DEC  N    E   C+N+ GG+ C C  GF    D+K C+
Sbjct: 1158 SCDSGFQSTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCSEGYALMPDQRSCA-DIDEC---EDNPDICDG------GQCTNIPGEYRCLCFDGFMASLDMKTCIDVNECDLNPNICL-HGECENTKGSFICHCQLGYFVKKGTTGCTDIDECEVGAHNCDVHASCLNVPGSFKCQCQTGW-VGDGLNCVDLDECTTEDHKCNA-NANCVNTPGSYRCACNEGFNGDGFICSDVDECADNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQ 1422          

HSP 3 Score: 120.168 bits (300), Expect = 2.721e-26
Identity = 95/264 (35.98%), Postives = 133/264 (50.38%), Query Frame = 3
Query: 2616 DINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D+NEC   +SL  H   R  N+ GSF C C +       E  C   I+EC     +      V      +C   +G E      GF   + + C+DI+EC  +P L +  TC N+ GSF+C CP G + +     CVDI+EC  S     NG C NMIG+Y+C+C +G++   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L   ++ C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1005 DVNECKVFNSLCTHGTCR--NSIGSFRCVCDSGFALDAEERNCT-DIDECRISPDLCGHGTCVNTPGSFDCECFDGYE-----SGFM--MMKNCMDIDECERDPLLCRGGTCINTDGSFECICPPGHELTSEGNACVDINECSLSDNLCRNGRCINMIGTYQCSCDSGFQSTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDQRSCADIDECEDNPDICDGGQCTNIPGEYRCLCFDGFMASLDMKTC 1255          

HSP 4 Score: 116.316 bits (290), Expect = 3.795e-25
Identity = 105/314 (33.44%), Postives = 147/314 (46.82%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   F T   D+D  + +  L         Q ++ +    C   +C  GY     G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++ECS ++                C ++ G   C CP GFT   +  CID NEC+  P  C  +  C+N+ GSF C C KGF   S    C D+DEC  +  C     CQN++G +RC C  GY ++ +   C+D +EC  S+  C   +  C NT GSFKCVCP G ++ +   GC D+DEC      P    C N+ GG+ C C  G+
Sbjct: 2370 HGPGFATDGRDIDECKVLPNLCKN-----GQCINTIGSFRC---HCKLGYTTDITGTTCVDLDECAQSPKPCNFICKNTEGSYQCSCPRG--YILQEDGKTCKDLDECSTKQHNCQF----------LCVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG--CQNVLGGFRCGCPQGYVQHYQWNQCVDENEC-TSQSTC--GSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGY 2657          

HSP 5 Score: 111.309 bits (277), Expect = 1.405e-23
Identity = 74/184 (40.22%), Postives = 101/184 (54.89%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D +  CID++EC+ +PC+    C N+ GS+ C C +G+Q +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC A+  +C   N +C N  GSFKC+C  G      G  CVD+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  451 CECNMGYKQDSRLECIDVDECSSSPCI-HGDCVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCRNGRCVNTEGSFQCICNAGFETTPDGKNCVDHDEC-ATTNMCL--NGMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGR-CINTEGSFRCECLGGLTIGIDGRVC 629          

HSP 6 Score: 110.153 bits (274), Expect = 3.206e-23
Identity = 104/300 (34.67%), Postives = 141/300 (47.00%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDA------VTCRSPANCSSSNG----VEFC----------VCPRG--FTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G Q+   G+ C+D NEC +      N RC+NT GS+ C C+   +  +    C    +   F   + ++C        +  +S   C+S  G     E C          +CP G  F  D +    DI+EC + P L K   C N+ GSF C C  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DEC   +  CQ    +C NT GSF C CP G    +  C+D +EC  Q + C     C N+ G F C C KGF  ++    CE
Sbjct: 2254 CPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTECVDTRQGFCFTEVLQTMCQLSSTNRNMVTKSECCCNSGRGWGNQCEICPLPGTGAFKKMCPHGPGFATDGR----DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCVDLDECAQSPKPC--NFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQ---FLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE 2544          

HSP 7 Score: 108.612 bits (270), Expect = 9.904e-23
Identity = 93/277 (33.57%), Postives = 127/277 (45.85%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G G+   G    DI+EC  L     N +CINT GSF C+C            C   ++EC+   K            P N  C ++ G   C CPRG+   +  + C D++EC+      +  C N+ GSF+C CP GF       C+D +EC     +C   G+CQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G F+C CP G    Y+   CVD +EC   +TC   S C N+ G F C C  GF F+     C+
Sbjct: 2371 GPGFATDGR---DIDECKVLPNLCKNGQCINTIGSFRCHCKLGYTTDITGTTCV-DLDECAQSPK------------PCNFICKNTEGSYQCSCPRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQH-QSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCEDVDECDGNHRCQHG----CQNVLGGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSAS-CYNTLGSFKCVCPSGFDFDQSFGGCQ 2625          

HSP 8 Score: 108.227 bits (269), Expect = 1.024e-22
Identity = 68/194 (35.05%), Postives = 105/194 (54.12%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +++G   C CP G+  D     C+D +EC++ NPC    TC N  G F+C C +GF+      C DI+EC +  +C+    C N  GSY C C +GY   ED+  C D+DEC      C+   ++C+N  G+F C+CP G++ +   +GC+D +EC+          C+N+ G + C CN+GF  ++    C
Sbjct: 2119 CINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC-GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLCAFR--CINTYGSYECTCPSGYTLREDRRMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMQRRPDGEGCMDENECRTKPGICVNGRCVNTVGSYKCDCNEGFQVSSSGTEC 2309          

HSP 9 Score: 99.7525 bits (247), Expect = 4.460e-20
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVV 3410
            GF  D++ G  +DI+EC+  P +     C N  GSF C CP GF Y+ +   C DIDEC   + +C  N  C N+ GSYRC C +GY+      C+  +ECQ    +C   +  C +T GS+ CVC  G +    +  C+DIDEC +   C  G TC N+ G +NC C  GF   ++    ++D+  ++V
Sbjct: 1728 GFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCS--HGTCMDTEGSYICVCHNGFKASGDQTMCMDIDECDRQ-PCGNG-TCKNTVGSYNCLCFPGFELTHNNDCMDIDECYSLV 1913          

HSP 10 Score: 97.8265 bits (242), Expect = 1.700e-19
Identity = 91/310 (29.35%), Postives = 134/310 (43.23%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDECQASKKLCQGDNVVCQNTPG-----------------------------SFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G NC+D++EC  +   C+ N+ C+NTPGS+ C C  NE +    F C+  ++EC+         D V      +C ++ G   C C  GFT  +  + C DI+EC         TC+N  G F C C  G++     G C D++EC D   C  NG+C N  G+Y C C   +E      GC+D  +  C    +      + C    G                              +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1327 CQTGWVGDGLNCVDLDECTTEDHKCNANANCVNTPGSYRCAC--NEGFNGDGFICS-DVDECA---------DNVNLCENGHCLNAPGGYRCECEMGFTPTEDSKACQDIDECTFQNICVFGTCQNLPGMFRCICDDGYELDRSGGNCTDVNECADPVNCI-NGLCVNTPGNYLCNCPQDFELNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGRAWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1623          

HSP 11 Score: 97.4413 bits (241), Expect = 1.978e-19
Identity = 63/155 (40.65%), Postives = 79/155 (50.97%), Query Frame = 3
Query: 2928 KATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
               C  +  S+ C C  G++      C+D+DEC  S    G+  C N  GSY C CH GY+    K  CIDIDEC  +  +C+  N  C NT GSF+C+C  G E    G  CVD DEC   N C  G  CIN  G F C C  GFV   + ++C
Sbjct:  439 NGRCIPTPASYRCECNMGYKQDSRLECIDVDECSSSPCIHGD--CVNTPGSYHCRCHEGYQSNPTKQACIDIDECIMNGVMCR--NGRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLNG-MCINEDGSFKCICKPGFVLAPNGRYC 588          

HSP 12 Score: 88.9669 bits (219), Expect = 7.327e-17
Identity = 98/297 (33.00%), Postives = 129/297 (43.43%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CPK---------------GFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        C+NT GSF C C     YLN E      I+ECS     I IC       P  C ++ G   CVCP  +   +    C+D+              NE + N  + K  C  SY     +      CP                GF   +  G  +DIDEC +      NG+C N IGS+RC C  G+ Y      C DIDEC + + LCQ  N  C N PGS++C C  G +    G CV  +ECQ+  N C  G TC+++ G + C C+ GF  + D   C
Sbjct: 1582 DIDECQELPGLCQGGNCVNTFGSFQCECPAGY-YLNEETRICEDIDECSAH---IGIC------GPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSN--MTKKMCCCSYNIGKAWNKPCEPCPSPATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQ-RNADCINIPGSYRCECSSGYKLSPSGACVGRNECQEIPNVCSHG-TCMDTEGSYICVCHNGFKASGDQTMC 1864          

HSP 13 Score: 83.1889 bits (204), Expect = 4.017e-15
Identity = 98/303 (32.34%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DECQAS--KKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY++     CID++EC S  C +   C+NTPGS+ C C    +  NP       I+EC        I + V CR+   C ++ G   C+C  GF T    + C+D +EC   N CL+   C N  GSF C C  GF  + +   CVD+DEC+   IC  NG C N  GS+RC C  G    ++ + C+D      C  +  K  C                    G    CQ  P      F+ +C  G+     G  DI+EC  N+  CP G  C N  G + C CN G+  +   K C
Sbjct:  453 CNMGYKQDSRLECIDVDECSSSPCIHGD-CVNTPGSYHCRCHEGYQS-NPTKQACIDIDEC--------IMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDHDECATTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICM-NGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG-RDINECALNSEICPNG-ICENLRGSYRCICNLGYESDASGKNC 740          

HSP 14 Score: 82.4185 bits (202), Expect = 8.351e-15
Identity = 90/308 (29.22%), Postives = 129/308 (41.88%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--------HF------------------GCVDIDECRDSKICSGNG-ICQ----NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C+D++EC          C N+ GSF C C  G    +        H                   G V   EC  +    G G  CQ         ++  C +G     D    DI+EC  + ++C   N +C+N  GS++C+C +G E    G  CVD+DEC  N    +   C N+ G + CTC KG+VF  D++ CE
Sbjct:  492 CHEGYQSNPTKQACIDIDECIMNGVMCRN-GRCVNTEGSFQCICNAGFETTPDGKNCVDH-DECATTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCVDVDECQTPGICMNGRCINTEGSFRCECLGGLTIGIDGRVCVDTHMRSTCYGAIKKGTCARPFPGAVTKSECCCASPDHGFGEPCQPCPAKNSAEFQALCSSGIGITADG--RDINECALNSEICP--NGICENLRGSYRCICNLGYESDASGKNCVDVDECAVNRLLCDNGLCRNTPGSYTCTCPKGYVFKIDSETCE 783          

HSP 15 Score: 80.4925 bits (197), Expect = 2.621e-14
Identity = 102/353 (28.90%), Postives = 135/353 (38.24%), Query Frame = 3
Query: 2457 ITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKI--GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINE-----------CNMNPCLDKATCR--------NSYG------------SFDCFCPKGFQYSVHFGCV--DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +T NND       Y L  +  +   Q ++ V    C    C +GY+    G NC+DINEC SL    +   C N  GSF C C    E  N        I EC  E  I              C+++ G   CVCP GF   D    C D  +           C++    +    R          +G            +F   CP G       G    D++EC +S     NG+C N  GS+RC C  GY        C+D DEC          N  C N  G F+C C  G E      C DI+EC     C     CIN++G + CTC  G+    D + C +LD+
Sbjct: 1897 LTHNNDCMDIDECYSLVGQVCRN-GQCINNVGSFQCL---CQEGYEITPDGKNCMDINECTSLPGTCSPGTCQNLEGSFRCICPPGFEVQNDN---CIDINECDEEPNICLF---------GTCTNTPGSFQCVCPPGFVLSDNGRRCFDTRQSFCFTRFDNGKCSVPKAFNTTKARCCCSKMPGEGWGDPCELCPQEGSVAFQELCPYGHGSIPGMGDTREDVNECSESPGICVNGVCINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPC---GNGTCSNVVGGFECTCDEGFEPGPMMTCEDINECTNPLLC--AFRCINTYGSYECTCPSGYTLREDRRMCKDLDE 2228          

HSP 16 Score: 80.4925 bits (197), Expect = 2.711e-14
Identity = 105/373 (28.15%), Postives = 143/373 (38.34%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKI-------------ISICDAVTCRSPANCSSSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTPGSFKCVCPVGLEY---------------KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            Q+L  +F   C D   G    + G NC D+NEC       N  C+NTPG+++C C  + E LNP   GC   ++  C  E +              + +  A  C S    +  N  EFC          +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P       P   EY               +    +DIDEC +         CIN  G F C C  GF +NN    C    E     N+ Q  AD +
Sbjct: 1439 QNLPGMFRCICDD---GYELDRSGGNCTDVNECADPVNCINGLCVNTPGNYLCNCPQDFE-LNPTGVGCVDTRVGNCFLETQPRGDGGLSCSAEIGVGVTRASCCCSLGR-AWGNPCEFCPFANSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECSAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNYNDTCENELSSNMTKKMCCCSYNIGKAWNKPCEPCPS------PATPEYIFLCGNQAPGFIIDIRTGKPMDIDECSEIPAICSNGVCINQIGSFRCECPMGFSYNNILLICEDIDECSSGENLCQRNADCI 1800          

HSP 17 Score: 78.5666 bits (192), Expect = 1.181e-13
Identity = 70/230 (30.43%), Postives = 93/230 (40.43%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK--------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   +T K+L +  +K        V  I   +  C  G+ +   +CID NEC S    C +   C N PGSF C C       +    C   ++EC    +    C  V            G   C CP+G+    +   C+D NEC        A+C N+ GSF C CP GF +   F GC D+DEC        +  C N  G Y C C  GY       C+
Sbjct: 2449 PRGYILQEDGKTCKDLDECSTKQHNCQFLCVNTIGSFNCKCPPGFTQHQSSCIDNNECSSQPSLCGSRGVCQNNPGSFTCECQKGFSLDSSGSNCE-DVDECDGNHRCQHGCQNVL-----------GGFRCGCPQGYVQHYQWNQCVDENECTSQSTCGSASCYNTLGSFKCVCPSGFDFDQSFGGCQDVDECSSGGGNPCSYGCSNTDGGYLCGCPGGYFRAGQGHCV 2666          

HSP 18 Score: 65.0846 bits (157), Expect = 1.394e-9
Identity = 89/332 (26.81%), Postives = 132/332 (39.76%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC-IDIN----ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI-----------DECQAS------KKLC-----QGDNVVCQN--TPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+D++EC  + L C +N  C NTPGS+ C C     +      C   I EC+    +  +C            ++ G   C C  G   D         +K  C ++I     E N+N    K+ C  + G+           D  CP+G+       C D++EC        NG C N  GS++C C  G       + C+D+           DEC A         +C         + C+    PGS  FK +CP G  +  +G V        D++EC+  N+     TC NS G F C C+ GF  + + + C
Sbjct:  727 CNLGYESDASGKNCVDVDECAVNRLLC-DNGLCRNTPGSYTCTCPKGYVFKIDSETCE-DINECASNPCVNGVC-----------RNNAGSFVCECSFGSKLDSTGTICVDSMKGTCWLNIQDGRCEVNINGATLKSECCATLGAAWGSPCERCEIDPACPRGYSRKKGVACEDVNECEVFPGVCPNGRCVNTAGSFKCECPEGLTLDGSGRNCVDVRVEQCYMKWDEDECTAQLPGKYRMDMCCCSVGAAWGIDCEECPKPGSDEFKALCPRGPGFANRGDVLSGRPFYKDVNECKVFNSLCTHGTCRNSIGSFRCVCDSGFALDAEERNC 1045          

HSP 19 Score: 61.6178 bits (148), Expect = 1.444e-8
Identity = 37/92 (40.22%), Postives = 48/92 (52.17%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +    C D+DECQA   LCQG    C NT GS++C CP G +  E  + C DIDEC       +G  C N+ G + C C +GF  + D   C
Sbjct:  218 IRTGACQDVDECQAIPGLCQGG--TCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGECTNTAGSYVCVCPRGFTTSRDGSRC 307          

HSP 20 Score: 61.2326 bits (147), Expect = 2.021e-8
Identity = 42/104 (40.38%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    +C G G C N +GSY C C  G++  E  + C DIDEC     +C G    C NT GS+ CVCP G      G   ID+
Sbjct:  210 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GTCINTVGSYECKCPAGHKQSETSQKCEDIDECSTIPNVCDGGE--CTNTAGSYVCVCPRGFTTSRDGSRCIDQ 310          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Match: Hmcn1 (hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047])

HSP 1 Score: 154.451 bits (389), Expect = 9.140e-37
Identity = 100/269 (37.17%), Postives = 128/269 (47.58%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINEC--DSLACHNNSRCINTPGSFVC--YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDC--FCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNC 3338
            +C KG     D   C DI+EC      C     C NT GS+ C  +C       +    C   I EC          ++  C     C +  G   C C  G  Y LK   CID+NEC  N C     C+N+ G + C   CP G   + +  C+DIDEC+D +  C  N IC+N  GSYRCAC  GY      + CIDIDECQ ++  CQ +   C+NT GS++CVCP G  L    K C D+DEC +QN  C     C N  G + C
Sbjct: 5130 SCPKGLTIAADGRTCQDIDECALGGHTCRAGQDCDNTIGSYRCVVHCGTGFRRTSDGLSCQ-DINECQ---------ESSPCHQ--RCFNVIGSFHCGCEAG--YQLKGRKCIDVNECRQNVCRPDQHCKNTRGGYKCIDLCPSGMTKAENGTCIDIDECKDGTHQCRYNQICENTRGSYRCACPRGYRSQGVGRPCIDIDECQ-NRDTCQHE---CKNTIGSYQCVCPPGYRLMLNGKTCQDVDECLEQNVRCGPNRMCFNMRGSYQC 5380          

HSP 2 Score: 147.902 bits (372), Expect = 9.351e-35
Identity = 97/284 (34.15%), Postives = 138/284 (48.59%), Query Frame = 3
Query: 2577 CGKGY--QKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV--CPRGF--TYDLKEGCIDINECN-MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCA--CHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG----CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+    +G  C D +EC +   C +   C N  G++ C C            C   I+EC+            TCR+  +C ++ G   CV  C  GF  T D    C DINEC   +PC  +  C N  GSF C C  G+Q      C+D++ECR + +C  +  C+N  G Y+C   C +G    E+  CIDIDEC+     C+  N +C+NT GS++C CP G  Y+ +G    C+DIDECQ  +TC     C N+ G + C C  G+    + K C+
Sbjct: 5091 CPSGFILDSVGPFCADEDECTAGNPCSHT--CHNAIGAYYCSCPKGLTIAADGRTCQ-DIDECAL--------GGHTCRAGQDCDNTIGSYRCVVHCGTGFRRTSD-GLSCQDINECQESSPCHQR--CFNVIGSFHCGCEAGYQLKGR-KCIDVNECRQN-VCRPDQHCKNTRGGYKCIDLCPSGMTKAENGTCIDIDECKDGTHQCRY-NQICENTRGSYRCACPRG--YRSQGVGRPCIDIDECQNRDTCQH--ECKNTIGSYQCVCPPGYRLMLNGKTCQ 5353          

HSP 3 Score: 138.658 bits (348), Expect = 5.591e-32
Identity = 85/231 (36.80%), Postives = 116/231 (50.22%), Query Frame = 3
Query: 2571 VNCGKGYQKIGD--NCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV--CPRGFTYDLKEGCIDINECN--MNPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE-YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV---CP 3227
            V+CG G+++  D  +C DINEC +S  CH   RC N  GSF C C         E G   K  +C      ++ C    CR   +C ++ G   C+  CP G T      CIDI+EC    + C     C N+ GS+ C CP+G++   V   C+DIDEC++   C     C+N IGSY+C C  GY   +  K C D+DEC      C G N +C N  GS++C+   CP
Sbjct: 5174 VHCGTGFRRTSDGLSCQDINECQESSPCHQ--RCFNVIGSFHCGC---------EAGYQLKGRKCI----DVNECRQNVCRPDQHCKNTRGGYKCIDLCPSGMTKAENGTCIDIDECKDGTHQCRYNQICENTRGSYRCACPRGYRSQGVGRPCIDIDECQNRDTCQHE--CKNTIGSYQCVCPPGYRLMLNGKTCQDVDECLEQNVRC-GPNRMCFNMRGSYQCIDTPCP 5386          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Match: Fbn2 (fibrillin 2 [Source:MGI Symbol;Acc:MGI:95490])

HSP 1 Score: 145.976 bits (367), Expect = 2.807e-34
Identity = 105/266 (39.47%), Postives = 150/266 (56.39%), Query Frame = 3
Query: 2610 CIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC   D+L C  N+ CIN+PGS+ C C+   + L+P   C  +  EC    +I ++C      S   C    G   C+C  GF     +  C+D++EC  +PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N IGS++C C+ GYE   D K CID +EC A    C      CQN  GSF+C+CP G E + + C+DI+EC ++ N C  GS C N+ GGF C C  GFV +++ + C
Sbjct: 1841 CEDIDECSNGDNL-CQRNADCINSPGSYRCECAAGFK-LSPNGACVDR-NEC---LEIPNVC------SHGLCVDLQGSYQCICNNGFKASQDQTMCMDVDECERHPC-GNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCR-NGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPG--TCQNLEGSFRCICPPGYEVRSENCIDINECDEDPNICLFGS-CTNTPGGFQCICPPGFVLSDNGRRC 2089          

HSP 2 Score: 125.561 bits (314), Expect = 4.167e-28
Identity = 101/281 (35.94%), Postives = 140/281 (49.82%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH---FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC  +    +++C N+ GS+ C CS     +     CA  I+EC        ICD         C++  G   C+C  GF  + D+K  CID+NEC++NP  C+    C N+ GSF C C  G  YSV     GC D+DEC   +  C  +  C N+ GS++C+C  G+     K CID+DEC      C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N  G + C C  GF   +D++ C+
Sbjct: 1219 SCNPGYQATPDRQGCTDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCA-DIDEC---ENNPDICDG------GQCTNIPGEYRCLCYDGFMASMDMKT-CIDVNECDLNPNICM-FGECENTKGSFICHCQLG--YSVKKGTTGCTDVDECEIGAHNCDMHASCLNVPGSFKCSCREGWVGNGIK-CIDLDECANGTHQCS-INAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENTNLCENGQCLNVPGAYRCECEMGFTPASDSRSCQ 1483          

HSP 3 Score: 122.479 bits (306), Expect = 3.880e-27
Identity = 106/305 (34.75%), Postives = 143/305 (46.89%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSF---ERKIISIC------DAVTCRSPA-------NCSSSNGVEF-CVCPRGFTYDLKEGCI-------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ +I G  C D+NEC+       N RC+N+ GSF C C            C   ++E C     E + I         DA  C   A        C      E+  +CPRG  +  +   +       DINEC   P +     CRN+ GSF C C  GF   +    C DIDECR S    G+GIC N  GS+ C C  GYE  ++  K C+DIDEC+ +  LC+G    C NT GSF+C CP+G E     + CVDI+EC  ++       C+N  G + C+CN G+    D + C
Sbjct:  931 DAACPRGFARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEHCFLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGPGFANRGDILTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNCTDIDECRISPDLCGSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGG--TCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGC 1233          

HSP 4 Score: 122.094 bits (305), Expect = 5.668e-27
Identity = 97/264 (36.74%), Postives = 129/264 (48.86%), Query Frame = 3
Query: 2616 DINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC +        +C NT GSF C C+N       E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  TC N+ GSF C CP G + S     CVDI+EC  S     NG C NMIG+Y+C+C+ GY+   D+ GC DIDEC      C   +  C N+ GS++C C  G      G  C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1066 DINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNCT-DIDECRISPDLC---------GSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCTDIDECMIMNGGC---DTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTC 1316          

HSP 5 Score: 114.775 bits (286), Expect = 9.158e-25
Identity = 98/295 (33.22%), Postives = 133/295 (45.08%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G   +  G+ C+D NEC +      N RC+N  GS+ C C+   +  +    C    +   F   + ++C   +       +S   C    G     E C          +CP G  Y       DI+EC + P L     C N+ GSF CFC  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  Q + C     C N+ G F+C C +GF  +     CE
Sbjct: 2319 GMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYATDG--RDIDECKVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISGTACVDLDECSQSPKPC--NFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQ---FLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCE 2606          

HSP 6 Score: 112.079 bits (279), Expect = 6.137e-24
Identity = 98/320 (30.63%), Postives = 148/320 (46.25%), Query Frame = 3
Query: 2604 DNCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNN-----------DTKFCELDKTLNVVQP-KADLVFGIDTSESMKNYMDIVKDMLKNI 3500
            ++C+DINEC   D+L    N +C+N  G++ C C+   +      GC   I+EC     +   CD         C++S G   C C  G+   + +G  C DI+EC  NP + D   C N  G + C C  GF  S+    C+D++EC  +      G C+N  GS+ C C  GY   +   GC D+DEC+     C   +  C N PGSFKC C  G       C+D+DEC    + C   + C+N+ G + C C++GF  +            +T  CE  + LNV    + +   G   +   ++  DI +   +NI
Sbjct: 1189 EDCVDINECSLSDNLC--RNGKCVNMIGTYQCSCNPGYQATPDRQGCT-DIDECMI---MNGGCDT-------QCTNSEGSYECSCSEGYAL-MPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTCIDVNECDLNPNICMFGECENTKGSFICHCQLGYSVKKGTTGCTDVDECEIGAHNCD-MHASCLNVPGSFKCSCREGWVGNGIKCIDLDECANGTHQCSINAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENTNLCENGQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNI 1493          

HSP 7 Score: 112.079 bits (279), Expect = 6.676e-24
Identity = 74/184 (40.22%), Postives = 93/184 (50.54%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D    CID++EC  NPC     C N+ GS+ C C  GFQ +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC  +     G   +C N  GSFKCVC  G      G  C D+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  512 CECNMGYKQDANGDCIDVDECTSNPC-SNGDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNCVDHDECTTTNMCLNG---MCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICMNGH-CINNEGSFRCDCPPGLAVGVDGRVC 690          

HSP 8 Score: 110.923 bits (276), Expect = 1.389e-23
Identity = 91/277 (32.85%), Postives = 124/277 (44.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DI+EC  + +   N +C+NT GSF C+C            C   ++ECS   K            P N  C ++ G   C CPRG+   +  + C D++EC       +  C N+ G F C CP GF    H  C+D +EC     +C   GICQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC     C   S C N+ G + C C  GF F+  +  C 
Sbjct: 2433 GPGYATDGR---DIDECKVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISGTACV-DLDECSQSPK------------PCNFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCEDVDECDGNHRCQHG----CQNILGGYRCGCPQGYVQHYQWNQCVDENECSNPGACGSAS-CYNTLGSYKCACPSGFSFDQFSSACH 2687          

HSP 9 Score: 105.145 bits (261), Expect = 8.438e-22
Identity = 89/260 (34.23%), Postives = 122/260 (46.92%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGF 3356
            G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++EC  ++                C ++ G   C CP GFT      CID NEC   P  C  K  C+N+ GSF C C +GF        C D+DEC  +  C     CQN++G YRC C  GY ++ +   C+D +EC          +  C NT GS+KC CP G  + +    C D++EC  + N C  G  C N+ GG+ C C  G+
Sbjct: 2479 GTACVDLDECSQSPKPCNFICKNTKGSYQCSCPRG--YVLQEDGKTCKDLDECQTKQHNCQFL----------CVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCEDVDECDGNHRCQHG--CQNILGGYRCGCPQGYVQHYQWNQCVDENECSNPGAC---GSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCSYG--CSNTEGGYLCGCPPGY 2718          

HSP 10 Score: 98.5969 bits (244), Expect = 8.004e-20
Identity = 98/317 (30.91%), Postives = 139/317 (43.85%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK--------------------------EGCIDINEC-----------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G++    G NC+D +EC +     N  CIN  GSF C C        P F  AP    C+         D   C++P      +C ++ G   C CP G    +                            G +  +EC              PC  K +       F   C  G   +V     DI+EC  D  IC+ NGIC+N+ GSYRC C++GYE     + CIDIDEC  ++ LC  DN +C+NTPGS+ C CP G  ++   + C D++EC ++N C  G+ C N+ G F+C C+ G   ++    C
Sbjct:  595 CNAGFELTTDGKNCVDHDECTTTNMCLNGMCINEDGSFKCVCK-------PGFILAPNGRYCT---------DVDECQTPGICMNGHCINNEGSFRCDCPPGLAVGVDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNS-----AEFHGLCSSGIGITVDG--RDINECALDPDICA-NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLC--DNGLCRNTPGSYSCTCPPGYVFRTETETCEDVNEC-ESNPCVNGA-CRNNLGSFHCECSPGSKLSSTGLIC 883          

HSP 11 Score: 98.2117 bits (243), Expect = 8.348e-20
Identity = 66/185 (35.68%), Postives = 101/185 (54.59%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GFT+D+  G  +DI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC +   +C  N  C N  GSYRC C  G++   +  C+D +EC     +C   + +C +  GS++C+C  G +  +    C+D+DEC+++  C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1789 GFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCS--HGLCVDLQGSYQCICNNGFKASQDQTMCMDVDECERH-PCGNG-TCKNTVGSYNCLCYPGFELTHNNDCLDIDE 1969          

HSP 12 Score: 94.3597 bits (233), Expect = 1.434e-18
Identity = 95/297 (31.99%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP----------------RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF------CP--------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        CINT GSF C C     YL+ E      I+EC     +           P  C ++ G   C+CP                + F Y    G    NE   N  + K  C  +Y     +      CP               GF + +H G  VDIDEC++      NG+C N IGS+RC C  G+ Y +    C DIDEC     LCQ  N  C N+PGS++C C  G +    G CVD +EC +  N C  G  C++  G + C CN GF  + D   C
Sbjct: 1643 DIDECQELPGLCQGGNCINTFGSFQCECPQGY-YLSEETRICEDIDECFAHPGVCG---------PGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFN--VTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQ-RNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHG-LCVDLQGSYQCICNNGFKASQDQTMC 1925          

HSP 13 Score: 92.4337 bits (228), Expect = 4.817e-18
Identity = 92/311 (29.58%), Postives = 136/311 (43.73%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDEC--------------------QASKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C +G+   G  CID++EC   +  C  N++C+NTPGS+ C CS  E +    F C+  ++EC+   +  ++C+         C +  G   C C  GFT       C DI+EC+        TC N  G F C C  G++     G C DIDEC D   C  NG+C N  G Y C C   ++      GC+D  +  C                      S+  C     +     C+  P      +  +CP G  ++         DIDECQ+     +G  CIN+FG F C C +G+  + +T+ CE
Sbjct: 1387 SCREGWVGNGIKCIDLDECANGTHQCSINAQCVNTPGSYRCACS--EGFTGDGFTCS-DVDECA---ENTNLCEN------GQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYELDRTGGNCTDIDECADPINCV-NGLCVNTPGRYECNCPPDFQLNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEVGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEETRICE 1684          

HSP 14 Score: 88.5817 bits (218), Expect = 7.998e-17
Identity = 86/273 (31.50%), Postives = 121/273 (44.32%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNG-ICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            D+NEC +S    +N +CINT GSF C C            C    +ECS    I + C   TC      ++  G   C C  GF       C DINEC  NP L    C N++GS++C CP G+        C D+DEC +    C   G +C+N+IG++ C C  G     D +GC+D +EC+    +C+  N  C N  GS++C C  G +    G   +D  Q           C   S+  N      C C+ G  + +  + C L  T
Sbjct: 2164 DVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCV-DTDECS----IGNPCGNGTC------TNVIGSFECTCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPVGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICE--NGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGT 2423          

HSP 15 Score: 83.9593 bits (206), Expect = 1.874e-15
Identity = 91/297 (30.64%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2604 DNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDI-------DECQ--------ASKKLCQ-GDNVV------------CQNTPG------------SFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            ++C+DI+EC S       N RC N  GSF C C+   E         P  + C    + +++  +    SP  C +  G   C+CP G+     E CIDINEC+ +P  CL   +C N+ G F C CP GF  S +        C D++       C     + +C+    +   + K C          D C+        A + LC  G   V            C  +PG            SF+C CP+G  L+Y    CVD DEC   N C  G TC N  G F CTCN+GF
Sbjct: 1962 NDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYEL-------TPDGKNCIDTNECVALPGSC---SPGTCQNLEGSFRCICPPGYEV-RSENCIDINECDEDPNICL-FGSCTNTPGGFQCICPPGFVLSDNG-----RRCFDTR----QSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCVDTDECSIGNPCGNG-TCTNVIGSFECTCNEGF 2236          

HSP 16 Score: 82.4185 bits (202), Expect = 6.137e-15
Identity = 94/309 (30.42%), Postives = 128/309 (41.42%), Query Frame = 3
Query: 2607 NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGYEYVED-------------KGCI----------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPK 3419
            NC DINEC         RC+NT GS+ C C         +  C   ++EC+   + +  C++        C +  G   C+CP G       EGC+D NEC   P + +   C N  GS+ C C +GFQ S         EC D++           +C      +N++    C C  G  +                K C           DIDEC+    LC   N  C NT GSF+C C VG      G  CVD+DEC Q+   P    C N+ G + C+C +G+V   D K C   K L+  Q K
Sbjct: 2243 NCEDINECAQNPLLCAFRCMNTFGSYECTCPVGYALREDQKMCK-DLDECA---EGLHDCESRG----MMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSG-----TECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYATDGRDIDECKVMPSLCT--NGQCVNTMGSFRCFCKVGYTTDISGTACVDLDECSQSPK-PCNFICKNTKGSYQCSCPRGYVLQEDGKTC---KDLDECQTK 2532          

HSP 17 Score: 81.6481 bits (200), Expect = 1.042e-14
Identity = 105/344 (30.52%), Postives = 139/344 (40.41%), Query Frame = 3
Query: 2409 LTEINLLKDEGHRLT-----FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCID--INECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY--EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +     L +EG+ LT      I  N  V +P S        +    Q+L   F   C       GY+   +NCIDINECD     C   S C NTPG F C C        P F  +     C   R+     +             NG   C  P+ F     + C      E   +PC  +   ++   +F   CP G     S+H    D++EC +S     NG C N  GS+RC C  GY  +Y   + C+D DEC          N  C N  GSF+C C  G E      C DI+EC QN        C+N+FG + CTC  G+    D K C +LD+
Sbjct: 1987 IGSFKCLCNEGYELTPDGKNCIDTNECVALPGSC-------SPGTCQNLEGSFRCICP-----PGYEVRSENCIDINECDEDPNICLFGS-CTNTPGGFQCICP-------PGFVLSDNGRRCFDTRQSFCFTNF-----------ENGK--CSVPKAFNTTKAKCCCSKMPGEGWGDPC--ELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVR-CVDTDECSIGNPC---GNGTCTNVIGSFECTCNEGFEPGPMMNCEDINECAQNPL-LCAFRCMNTFGSYECTCPVGYALREDQKMCKDLDE 2290          

HSP 18 Score: 80.4925 bits (197), Expect = 2.167e-14
Identity = 51/125 (40.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 3015 IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID C+       NG C   + SYRC C+ GY+   +  CID+DEC  S     GD   C NTPGS+ C C  G +    ++ C+DIDEC QN    +   C+N+ G F C CN GF    D K C
Sbjct:  488 IDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDEC-TSNPCSNGD---CVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNC 608          

HSP 19 Score: 79.337 bits (194), Expect = 5.748e-14
Identity = 91/312 (29.17%), Postives = 127/312 (40.71%), Query Frame = 3
Query: 2577 CGKGYQKIG--DNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI---CSGN---GIC----QNMIGSYRCACHNGYEYVEDKGCI-------------------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q+      CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   CVC  GF        C D++EC          C N+ GSF C CP G         VD   C D+ +   C G    G+C       +    C C N  +Y   + C                          DI+EC     +C   N +C+N  GS++C C  G E    G  C+DIDEC  N    +   C N+ G ++CTC  G+VF  +T+ CE
Sbjct:  553 CHAGFQRTPTKQACIDIDECIQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKNCVDH-DECTTTNMCLN----------GMCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICMNGHCINNEGSFRCDCPPGLAVG-----VDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCAN-PDYGFGEPCQPCPAKNSAEFHGLCSSGIGITVDGRDINECALDPDICA--NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCE 844          

HSP 20 Score: 78.1814 bits (191), Expect = 1.256e-13
Identity = 97/310 (31.29%), Postives = 137/310 (44.19%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DEC--QASKKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTL 3401
            C  GY Q    +CID++EC S  C +N  C+NTPGS+ C C    +    +  C   I+EC        I + V C++   C +++G   C+C  GF  T D K  C+D +EC   N CL+   C N  GSF C C  GF  + +   C D+DEC+   IC  NG C N  GS+RC C  G    V+ + C+D      C  +  K +C                    G    CQ  P      F  +C  G+     G  DI+EC  +        C N  G + C CN G+  +   + C ++D+ L
Sbjct:  514 CNMGYKQDANGDCIDVDECTSNPC-SNGDCVNTPGSYYCKCHAGFQRTPTKQACI-DIDEC--------IQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKN-CVDHDECTTTNMCLN-GMCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICM-NGHCINNEGSFRCDCPPGLAVGVDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNSAEFHGLCSSGIGITVDG-RDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECL 808          

HSP 21 Score: 73.559 bits (179), Expect = 2.594e-12
Identity = 106/366 (28.96%), Postives = 148/366 (40.44%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGC------------APKIE---ECSFERKI---ISICDAVTCRSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-----------------QASKKLC-------QGDNVVCQN--TPGS--FKCVCP----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            +L  +F   C D   G    + G NC DI+EC D + C N   C+NTPG + C C  + + LNP   GC             P+ +    C+ E  +    S C     ++  N    C   N  E+  +CP G  +      I   DI+EC   P L     C N++GSF C CP+G+  S     C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +                    +K++C       +  N  C+   TPG+  FK +C        +      VDIDEC++         CIN  G F C C  GF +N+    C    E     N+ Q  AD +
Sbjct: 1501 NLPGMFHCICDD---GYELDRTGGNCTDIDECADPINCVN-GLCVNTPGRYECNCPPDFQ-LNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEVGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEETRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCI 1861          

HSP 22 Score: 73.559 bits (179), Expect = 3.043e-12
Identity = 83/301 (27.57%), Postives = 117/301 (38.87%), Query Frame = 3
Query: 2304 KIVLSQCNGAR----LGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINN----DVDIPRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            K++ S C   +    +GS R    VG  T+++         +++          FI  N         PR  + Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC S    C     C NTPGSF C C            C   ++EC    +    C  +            G   C CP+G+    +   C+D NEC+       A+C N+ GS+ C CP GF +      C D++EC  SK     G C N  G Y C C  GY  V    C+
Sbjct: 2447 KVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISG----TACVDLDECSQSPKPCNFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCK---CPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCE-DVDECDGNHRCQHGCQNIL-----------GGYRCGCPQGYVQHYQWNQCVDENECSNPGACGSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCSYG-CSNTEGGYLCGCPPGYFRVGQGHCV 2727          

HSP 23 Score: 69.3218 bits (168), Expect = 6.280e-11
Identity = 68/216 (31.48%), Postives = 95/216 (43.98%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGS----------------------FKCVCPVGL-EYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +  G   C C  G+  D     CIDI+EC +N  L D   CRN+ GS+ C CP G+ +      C D++EC +S  C  NG C+N +GS+ C C  G + +   G I ID  + +  L   DN    N  G+                          CP G    K   C D++EC+     CP G  C+NS G F+C C +G   +   + C
Sbjct:  777 CENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCEDVNEC-ESNPCV-NGACRNNLGSFHCECSPGSK-LSSTGLICIDSLKGTCWLNIQDNRCEVNINGATLKSECCATLGAAWGSPCERCELDAACPRGFARIKGVTCEDVNECEVFPGVCPNGR-CVNSKGSFHCECPEGLTLDGTGRVC 988          

HSP 24 Score: 63.5438 bits (153), Expect = 3.028e-9
Identity = 42/100 (42.00%), Postives = 52/100 (52.00%), Query Frame = 3
Query: 3093 CHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+   +    C D+DECQA   LCQG N  C NT GSF+C CP G +  E  + C DIDEC       E   C N+ G + C C +GFV + D   C
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGLCQGGN--CINTVGSFECRCPAGHKQSETTQKCEDIDECSVIPGVCETGDCSNTVGSYFCLCPRGFVTSTDGSRC 358          

HSP 25 Score: 56.9954 bits (136), Expect = 2.958e-7
Identity = 89/333 (26.73%), Postives = 130/333 (39.04%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC---IDINEC--NMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDK------GCIDIDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  GY+    G NCIDI+EC  + L C +N  C NTPGS+ C C        P +    + E C    + ++ C++  C + A C ++ G   C C  G             LK  C   I  N C  N+N    K+ C  + G+           D  CP+GF       C D++EC        NG C N  GS+ C C  G                    ++ ED+      G   +D C  +     G        PG+  ++ +CP G  +  +G +        DI+EC+          C N+ G F C CN GF  + + + C
Sbjct:  787 NCNSGYEPDASGRNCIDIDECLVNRLLC-DNGLCRNTPGSYSCTCP-------PGYVFRTETETC----EDVNECESNPCVNGA-CRNNLGSFHCECSPGSKLSSTGLICIDSLKGTCWLNIQDNRCEVNINGATLKSECCATLGAAWGSPCERCELDAACPRGFARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEHCFLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGPGFANRGDILTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNC 1106          

HSP 26 Score: 55.0694 bits (131), Expect = 1.321e-6
Identity = 45/115 (39.13%), Postives = 61/115 (53.04%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEG 3302
            C +GF  ++  G C D+DEC+    +C G G C N +GS+ C C  G++  E  + C DIDEC     +C+ GD   C NT GS+ C+CP G      G   ID  Q+  TC  G
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GNCINTVGSFECRCPAGHKQSETTQKCEDIDECSVIPGVCETGD---CSNTVGSYFCLCPRGFVTSTDGSRCID--QRAGTCFSG 369          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Match: Ltbp1 (latent transforming growth factor beta binding protein 1 [Source:MGI Symbol;Acc:MGI:109151])

HSP 1 Score: 144.436 bits (363), Expect = 7.119e-34
Identity = 108/305 (35.41%), Postives = 146/305 (47.87%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVC-------------YCSNNEEYLNPE-------------FGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            G NCID++EC       + RCINT G+F C             YC + +E L P              + C P  E   F        D   C  P      +C++  G   C C RG+  T D +  C DI+EC          CRN+ GSF C C +G+Q S     C DIDEC    +CS +G C+N  GS++C C+ GY   V    C DI+EC     +CQG +  C NT GS+ C CP G +  + KGC DI+EC Q   C     C+N+ G F+C C +GF  + D + CE +D+ +N
Sbjct:  944 GTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTE--GFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYSPTPDHRH-CQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCS-HGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGD--CINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVN 1242          

HSP 2 Score: 138.658 bits (348), Expect = 4.187e-32
Identity = 99/277 (35.74%), Postives = 135/277 (48.74%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            V C +G++     C+D++EC       N  C N  GS++C C        P+      I+EC      ++            C +++G   C C +G+     K+ C DI+EC  +       CRN+ GSF C C +G++ SV  G  C DI+EC  DS +C G G C N  GSY C C +G++  ++KGC DI+EC A   LC G +  C NT GSF CVC  G      G  C DIDEC  N  C     C N+ G F C C +GF    D + C
Sbjct: 1015 VPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYS-PTPDHRHCQDIDECQQGNLCMN----------GQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQCRNTEGSFQCVCNQGYRASV-LGDHCEDINECLEDSSVCQG-GDCINTAGSYDCTCPDGFQLNDNKGCQDINEC-AQPGLC-GSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGC 1276          

HSP 3 Score: 122.865 bits (307), Expect = 2.893e-27
Identity = 94/265 (35.47%), Postives = 132/265 (49.81%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTC 3344
            +C +GY    D+  C DI+EC       N +C NT GSF C C    +    +  C   I+EC        +C    CR+      + G   CVC +G+    L + C DINEC  +  +     C N+ GS+DC CP GFQ + + GC DI+EC    +C  +G C N  GS+ C C  G+    D + C DIDEC  +  +C      C NT GSF+C+C  G +  +  +GCVD++EC+  +  C E + C N  G F C C
Sbjct: 1055 SCHRGYSPTPDHRHCQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCE-DIDECEHHH----LCSHGQCRN------TEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDEC-VNNTVCDSHG-FCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGE-AFCENVEGSFLCVC 1305          

HSP 4 Score: 119.783 bits (299), Expect = 2.556e-26
Identity = 96/271 (35.42%), Postives = 132/271 (48.71%), Query Frame = 3
Query: 2610 CIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFC-VCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C+DI+EC  +  H  +  RC NT GSF+C C     ++  E G               +  D   C  P       C ++ G   C  C  G+    +  C DI+EC       +  C N+ GS+ C  C +GF+   +  C+D+DEC   K+C+ NG C N+ GSY C+CH GY    D + C DIDECQ    LC   N  C+NT GSF+C C  G  L   +  C DIDEC+ ++ C  G  C N+ G F C CN+G+  +     CE
Sbjct:  905 CVDIDECAQVR-HLCSQGRCENTEGSFLCVCPAG--FMASEEG--------------TNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTEGFR-GWNGQCLDVDECLQPKVCT-NGSCTNLEGSYMCSCHRGYSPTPDHRHCQDIDECQQG-NLCM--NGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQ-CRNTEGSFQCVCNQGYRASVLGDHCE 1152          

HSP 5 Score: 86.6557 bits (213), Expect = 2.853e-16
Identity = 61/161 (37.89%), Postives = 87/161 (54.04%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCT-CNKGF 3356
            +INEC +NP +     C N    + C C +G+++S     CVDIDEC   +     G C+N  GS+ C C  G+   E+   CID+DEC     +C+  +  C NT G+F+C  C  G     +G C DIDEC + +TCPE   C+N+ G + C  C +GF
Sbjct:  865 EINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCIDVDEC-LRPDMCR--DGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPE-EQCVNTPGSYQCVPCTEGF 1021          

HSP 6 Score: 71.633 bits (174), Expect = 9.006e-12
Identity = 96/341 (28.15%), Postives = 135/341 (39.59%), Query Frame = 3
Query: 2550 FDITCKDVNCGKGYQKIGDN--CIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFG----------CVDIDECRDSKICSGN----GICQNMIGS---------------------YRCACHNGYEYVE----DKGCI---------------DIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +D TC D     G+Q + DN  C DINEC     C ++  C+NT GSF C C            C   I+EC          +   C S   C ++ G   C+C +GF      +GC+D+NEC + +    +A C N  GSF C C    Q YS   G           VD     + K C  N     +C N++                       + C      E+ E     KG +               D DEC    +++C+  N  C NT   ++C C  G  Y   +  C D+DECQ  N+C +G  C+N+ G +NC C    V +   K C
Sbjct: 1176 YDCTCPD-----GFQ-LNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCE-DIDECV---------NNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRVTEDSGVDRQPREEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVPAGESSYDTGGENYKDADECLLFGEEICK--NGYCLNTQPGYECYCKQGTYYDPVKLQCFDMDECQDPNSCIDGQ-CVNTEGSYNCFCTHPMVLDASEKRC 1497          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Match: Ltbp1 (latent transforming growth factor beta binding protein 1 [Source:MGI Symbol;Acc:MGI:109151])

HSP 1 Score: 144.436 bits (363), Expect = 7.121e-34
Identity = 108/305 (35.41%), Postives = 146/305 (47.87%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVC-------------YCSNNEEYLNPE-------------FGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            G NCID++EC       + RCINT G+F C             YC + +E L P              + C P  E   F        D   C  P      +C++  G   C C RG+  T D +  C DI+EC          CRN+ GSF C C +G+Q S     C DIDEC    +CS +G C+N  GS++C C+ GY   V    C DI+EC     +CQG +  C NT GS+ C CP G +  + KGC DI+EC Q   C     C+N+ G F+C C +GF  + D + CE +D+ +N
Sbjct:  945 GTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTE--GFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYSPTPDHRH-CQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCS-HGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGD--CINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVN 1243          

HSP 2 Score: 138.658 bits (348), Expect = 4.153e-32
Identity = 99/277 (35.74%), Postives = 135/277 (48.74%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            V C +G++     C+D++EC       N  C N  GS++C C        P+      I+EC      ++            C +++G   C C +G+     K+ C DI+EC  +       CRN+ GSF C C +G++ SV  G  C DI+EC  DS +C G G C N  GSY C C +G++  ++KGC DI+EC A   LC G +  C NT GSF CVC  G      G  C DIDEC  N  C     C N+ G F C C +GF    D + C
Sbjct: 1016 VPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYS-PTPDHRHCQDIDECQQGNLCMN----------GQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQCRNTEGSFQCVCNQGYRASV-LGDHCEDINECLEDSSVCQG-GDCINTAGSYDCTCPDGFQLNDNKGCQDINEC-AQPGLC-GSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGC 1277          

HSP 3 Score: 122.865 bits (307), Expect = 2.968e-27
Identity = 94/265 (35.47%), Postives = 132/265 (49.81%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTC 3344
            +C +GY    D+  C DI+EC       N +C NT GSF C C    +    +  C   I+EC        +C    CR+      + G   CVC +G+    L + C DINEC  +  +     C N+ GS+DC CP GFQ + + GC DI+EC    +C  +G C N  GS+ C C  G+    D + C DIDEC  +  +C      C NT GSF+C+C  G +  +  +GCVD++EC+  +  C E + C N  G F C C
Sbjct: 1056 SCHRGYSPTPDHRHCQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCE-DIDECEHHH----LCSHGQCRN------TEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDEC-VNNTVCDSHG-FCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGE-AFCENVEGSFLCVC 1306          

HSP 4 Score: 119.783 bits (299), Expect = 2.535e-26
Identity = 96/271 (35.42%), Postives = 132/271 (48.71%), Query Frame = 3
Query: 2610 CIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFC-VCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C+DI+EC  +  H  +  RC NT GSF+C C     ++  E G               +  D   C  P       C ++ G   C  C  G+    +  C DI+EC       +  C N+ GS+ C  C +GF+   +  C+D+DEC   K+C+ NG C N+ GSY C+CH GY    D + C DIDECQ    LC   N  C+NT GSF+C C  G  L   +  C DIDEC+ ++ C  G  C N+ G F C CN+G+  +     CE
Sbjct:  906 CVDIDECAQVR-HLCSQGRCENTEGSFLCVCPAG--FMASEEG--------------TNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTEGFR-GWNGQCLDVDECLQPKVCT-NGSCTNLEGSYMCSCHRGYSPTPDHRHCQDIDECQQG-NLCM--NGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQ-CRNTEGSFQCVCNQGYRASVLGDHCE 1153          

HSP 5 Score: 86.6557 bits (213), Expect = 2.902e-16
Identity = 61/161 (37.89%), Postives = 87/161 (54.04%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCT-CNKGF 3356
            +INEC +NP +     C N    + C C +G+++S     CVDIDEC   +     G C+N  GS+ C C  G+   E+   CID+DEC     +C+  +  C NT G+F+C  C  G     +G C DIDEC + +TCPE   C+N+ G + C  C +GF
Sbjct:  866 EINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCIDVDEC-LRPDMCR--DGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPE-EQCVNTPGSYQCVPCTEGF 1022          

HSP 6 Score: 71.633 bits (174), Expect = 9.084e-12
Identity = 96/341 (28.15%), Postives = 135/341 (39.59%), Query Frame = 3
Query: 2550 FDITCKDVNCGKGYQKIGDN--CIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFG----------CVDIDECRDSKICSGN----GICQNMIGS---------------------YRCACHNGYEYVE----DKGCI---------------DIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +D TC D     G+Q + DN  C DINEC     C ++  C+NT GSF C C            C   I+EC          +   C S   C ++ G   C+C +GF      +GC+D+NEC + +    +A C N  GSF C C    Q YS   G           VD     + K C  N     +C N++                       + C      E+ E     KG +               D DEC    +++C+  N  C NT   ++C C  G  Y   +  C D+DECQ  N+C +G  C+N+ G +NC C    V +   K C
Sbjct: 1177 YDCTCPD-----GFQ-LNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCE-DIDECV---------NNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRVTEDSGVDRQPREEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVPAGESSYDTGGENYKDADECLLFGEEICK--NGYCLNTQPGYECYCKQGTYYDPVKLQCFDMDECQDPNSCIDGQ-CVNTEGSYNCFCTHPMVLDASEKRC 1498          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Match: Ltbp1 (latent transforming growth factor beta binding protein 1 [Source:MGI Symbol;Acc:MGI:109151])

HSP 1 Score: 143.665 bits (361), Expect = 7.249e-34
Identity = 108/305 (35.41%), Postives = 146/305 (47.87%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVC-------------YCSNNEEYLNPE-------------FGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            G NCID++EC       + RCINT G+F C             YC + +E L P              + C P  E   F        D   C  P      +C++  G   C C RG+  T D +  C DI+EC          CRN+ GSF C C +G+Q S     C DIDEC    +CS +G C+N  GS++C C+ GY   V    C DI+EC     +CQG +  C NT GS+ C CP G +  + KGC DI+EC Q   C     C+N+ G F+C C +GF  + D + CE +D+ +N
Sbjct:  626 GTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTE--GFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYSPTPDHRH-CQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCS-HGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGD--CINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVN 924          

HSP 2 Score: 137.887 bits (346), Expect = 4.420e-32
Identity = 99/277 (35.74%), Postives = 135/277 (48.74%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            V C +G++     C+D++EC       N  C N  GS++C C        P+      I+EC      ++            C +++G   C C +G+     K+ C DI+EC  +       CRN+ GSF C C +G++ SV  G  C DI+EC  DS +C G G C N  GSY C C +G++  ++KGC DI+EC A   LC G +  C NT GSF CVC  G      G  C DIDEC  N  C     C N+ G F C C +GF    D + C
Sbjct:  697 VPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYS-PTPDHRHCQDIDECQQGNLCMN----------GQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQCRNTEGSFQCVCNQGYRASV-LGDHCEDINECLEDSSVCQG-GDCINTAGSYDCTCPDGFQLNDNKGCQDINEC-AQPGLC-GSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGC 958          

HSP 3 Score: 118.242 bits (295), Expect = 4.567e-26
Identity = 97/267 (36.33%), Postives = 134/267 (50.19%), Query Frame = 3
Query: 2610 CIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGC-APKIEECSFERKIISICDAVTCRSPANCSSSNGVEFC-VCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C+DI+EC  +  H  +  RC NT GSF+C C     ++  E G     ++EC              CR    C ++ G   C  C  G+    +  C DI+EC       +  C N+ GS+ C  C +GF+   +  C+D+DEC   K+C+ NG C N+ GSY C+CH GY    D + C DIDECQ    LC   N  C+NT GSF+C C  G  L   +  C DIDEC+ ++ C  G  C N+ G F C CN+G+  +     CE
Sbjct:  587 CVDIDECAQVR-HLCSQGRCENTEGSFLCVCPAG--FMASEEGTNCIDVDECLRPD---------MCRD-GRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTEGFR-GWNGQCLDVDECLQPKVCT-NGSCTNLEGSYMCSCHRGYSPTPDHRHCQDIDECQQG-NLCM--NGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQ-CRNTEGSFQCVCNQGYRASVLGDHCE 834          

HSP 4 Score: 85.8853 bits (211), Expect = 3.828e-16
Identity = 61/161 (37.89%), Postives = 86/161 (53.42%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCT-CNKGF 3356
            +INEC +NP +     C N    + C C +G+++S     CVDIDEC   +     G C+N  GS+ C C  G+   E+   CID+DEC     +C+     C NT G+F+C  C  G     +G C DIDEC + +TCPE   C+N+ G + C  C +GF
Sbjct:  547 EINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCIDVDEC-LRPDMCRDGR--CINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPE-EQCVNTPGSYQCVPCTEGF 703          
BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Match: sp|P35556|FBN2_HUMAN (Fibrillin-2 OS=Homo sapiens OX=9606 GN=FBN2 PE=1 SV=3)

HSP 1 Score: 146.747 bits (369), Expect = 1.300e-33
Identity = 106/266 (39.85%), Postives = 150/266 (56.39%), Query Frame = 3
Query: 2610 CIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC   D+L C  N+ CIN+PGS+ C C+   + L+P   C  +  EC    +I ++C      S   C    G   C+C  GF     +  C+D++EC  +PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N IGS++C C+ GYE   D K CID +EC A    C      CQN  GSF+C+CP G E K + C+DI+EC ++ N C  GS C N+ GGF C C  GFV +++ + C
Sbjct: 1848 CEDIDECSNGDNL-CQRNADCINSPGSYRCECAAGFK-LSPNGACVDR-NEC---LEIPNVC------SHGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERHPC-GNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCR-NGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPG--TCQNLEGSFRCICPPGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCPPGFVLSDNGRRC 2096          

HSP 2 Score: 122.479 bits (306), Expect = 3.241e-26
Identity = 106/305 (34.75%), Postives = 143/305 (46.89%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSF---ERKIISIC------DAVTCRSPA-------NCSSSNGVEF-CVCPRGFTYDLKEGCI-------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G  +I G  C D+NEC+       N RC+N+ GSF C C            C   ++E+C     E + I         DA  C   A        C      E+  +CPRG  +  +   +       DINEC   P +     CRN+ GSF C C  GF   +    C DIDECR S    G+GIC N  GS+ C C  GYE  ++  K C+DIDEC+ +  LC+G    C NT GSF+C CP+G E     + CVDI+EC  ++       C+N  G + C+CN G+    D + C
Sbjct:  938 DTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCTDIDECRISPDLCGSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGG--TCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGC 1240          

HSP 3 Score: 121.324 bits (303), Expect = 6.629e-26
Identity = 100/281 (35.59%), Postives = 139/281 (49.47%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH---FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC  +    +++C N+ GS+ C CS     +     CA  I+EC        ICD         C++  G   C+C  GF  + D+K  CID+NEC++N   C+    C N+ GSF C C  G  YSV     GC D+DEC   +  C  +  C N+ GS++C+C  G+     K CID+DEC      C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N  G + C C  GF   +D++ C+
Sbjct: 1226 SCNPGYQATPDRQGCTDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCA-DIDEC---ENNPDICDG------GQCTNIPGEYRCLCYDGFMASMDMKT-CIDVNECDLNSNICM-FGECENTKGSFICHCQLG--YSVKKGTTGCTDVDECEIGAHNCDMHASCLNIPGSFKCSCREGWIGNGIK-CIDLDECSNGTHQCS-INAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENINLCENGQCLNVPGAYRCECEMGFTPASDSRSCQ 1490          

HSP 4 Score: 120.168 bits (300), Expect = 1.463e-25
Identity = 96/264 (36.36%), Postives = 129/264 (48.86%), Query Frame = 3
Query: 2616 DINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC +        +C NT GSF C C++       E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  TC N+ GSF C CP G + S     CVDI+EC  S     NG C NMIG+Y+C+C+ GY+   D+ GC DIDEC      C   +  C N+ GS++C C  G      G  C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1073 DINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCT-DIDECRISPDLC---------GSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCTDIDECMIMNGGC---DTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTC 1323          

HSP 5 Score: 113.62 bits (283), Expect = 1.356e-23
Identity = 74/184 (40.22%), Postives = 94/184 (51.09%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D    CID++EC  NPC +   C N+ GS+ C C  GFQ +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC  +     G   +C N  GSFKC+C  G      G  C D+DECQ    C  G  CINS G F C C  G     D + C
Sbjct:  519 CECNMGYKQDANGDCIDVDECTSNPCTN-GDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNCVDHDECTTTNMCLNG---MCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGH-CINSEGSFRCDCPPGLAVGMDGRVC 697          

HSP 6 Score: 113.62 bits (283), Expect = 1.551e-23
Identity = 98/295 (33.22%), Postives = 133/295 (45.08%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G   +  G+ C+D NEC +      N RC+N  GS+ C C+   +  +    C    +   F   + +IC   +       +S   C    G     E C          +CP G  Y       DI+EC + P L     C N+ GSF CFC  G+   +    C+D+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  Q + C     C N+ G F+C C +GF  +     CE
Sbjct: 2326 GMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDG--RDIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPKPC--NYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQ---FLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE 2613          

HSP 7 Score: 111.694 bits (278), Expect = 5.256e-23
Identity = 93/277 (33.57%), Postives = 124/277 (44.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DI+EC  +     N +CINT GSF C+C            C   ++ECS   K            P N  C ++ G   C CPRG+   +  + C D++EC       +  C N+ G F C CP GF    H  C+D +EC     +C   GICQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC   N C   S C N+ G + C C  GF F+  +  C 
Sbjct: 2440 GPGYTTDGR---DIDECKVMPNLCTNGQCINTMGSFRCFCKVGYTTDISGTSCI-DLDECSQSPK------------PCNYICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG----CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSAS-CYNTLGSYKCACPSGFSFDQFSSACH 2694          

HSP 8 Score: 106.686 bits (265), Expect = 1.944e-21
Identity = 88/259 (33.98%), Postives = 122/259 (47.10%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            G +CID++EC       N  C NT GS+ C C     Y+  E G   K ++EC  ++                C ++ G   C CP GFT      CID NEC   P  C  K  C+N+ GSF C C +GF   +    C D+DEC  +  C     CQN++G YRC C  GY ++ +   C+D +EC          +  C NT GS+KC CP G  + +    C D++EC  +   P    C N+ GG+ C C  G+
Sbjct: 2486 GTSCIDLDECSQSPKPCNYICKNTEGSYQCSCPRG--YVLQEDGKTCKDLDECQTKQHNCQFL----------CVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCEDVDECDGNHRCQHG--CQNILGGYRCGCPQGYIQHYQWNQCVDENECSNPNAC---GSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKN-PCNYGCSNTEGGYLCGCPPGY 2725          

HSP 9 Score: 102.064 bits (253), Expect = 4.125e-20
Identity = 101/317 (31.86%), Postives = 139/317 (43.85%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK--------------------------EGCIDINEC-----------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G++    G NC+D +EC +     N  CIN  GSF C C        P F  AP    C+         D   C++P      +C +S G   C CP G    +                            G +  +EC              PC  K +       F   C  G   +V     DI+EC  D  IC+ NGIC+N+ GSYRC C++GYE     + CIDIDEC  ++ LC  DN +C+NTPGS+ C CP G  ++   + C DI+EC ++N C  G+ C N+ G FNC C+ G   ++    C
Sbjct:  602 CNAGFELTTDGKNCVDHDECTTTNMCLNGMCINEDGSFKCICK-------PGFVLAPNGRYCT---------DVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNS-----AEFHGLCSSGVGITVDG--RDINECALDPDICA-NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLC--DNGLCRNTPGSYSCTCPPGYVFRTETETCEDINEC-ESNPCVNGA-CRNNLGSFNCECSPGSKLSSTGLIC 890          

HSP 10 Score: 98.2117 bits (243), Expect = 6.301e-19
Identity = 66/185 (35.68%), Postives = 101/185 (54.59%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GFT+D+  G  +DI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC +   +C  N  C N  GSYRC C  G++   +  C+D +EC     +C   + +C +  GS++C+C  G +  +    C+D+DEC+++  C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1796 GFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCS--HGLCVDLQGSYQCICHNGFKASQDQTMCMDVDECERH-PCGNG-TCKNTVGSYNCLCYPGFELTHNNDCLDIDE 1976          

HSP 11 Score: 95.9005 bits (237), Expect = 3.506e-18
Identity = 94/311 (30.23%), Postives = 137/311 (44.05%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDEC--------------------QASKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C +G+   G  CID++EC   +  C  N++C+NTPGS+ C CS  E +    F C+  ++EC+   + I++C+         C +  G   C C  GFT       C DI+EC+        TC N  G F C C  G++     G C DIDEC D   C  NG+C N  G Y C C   ++      GC+D  +  C                      S+  C     +     C+  P      +  +CP G  ++         DIDECQ+     +G  CIN+FG F C C +G+  + DT+ CE
Sbjct: 1394 SCREGWIGNGIKCIDLDECSNGTHQCSINAQCVNTPGSYRCACS--EGFTGDGFTCS-DVDECA---ENINLCEN------GQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYELDRTGGNCTDIDECADPINCV-NGLCVNTPGRYECNCPPDFQLNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICE 1691          

HSP 12 Score: 91.2781 bits (225), Expect = 8.016e-17
Identity = 93/297 (31.31%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP----------------RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF------CP--------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        CINT GSF C C     YL+ +      I+EC     +           P  C ++ G   C+CP                + F Y    G    NE   N  + K  C  +Y     +      CP               GF + +H G  VDIDEC++      NG+C N IGS+RC C  G+ Y +    C DIDEC     LCQ  N  C N+PGS++C C  G +    G CVD +EC +  N C  G  C++  G + C C+ GF  + D   C
Sbjct: 1650 DIDECQELPGLCQGGNCINTFGSFQCECPQGY-YLSEDTRICEDIDECFAHPGVCG---------PGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFN--VTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQ-RNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHG-LCVDLQGSYQCICHNGFKASQDQTMC 1932          

HSP 13 Score: 87.0409 bits (214), Expect = 1.510e-15
Identity = 83/273 (30.40%), Postives = 118/273 (43.22%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNG-ICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            D+NEC +S    +N +CINT GSF C C            C    +ECS      +            C++  G   C C  GF       C DINEC  NP L    C N++GS++C CP G+        C D+DEC +    C   G +C+N+IG++ C C  G     D +GC+D +EC+    +C+  N  C N  GS++C C  G +    G   +D  Q           C   S+  N      C C+ G  + +  + C L  T
Sbjct: 2171 DVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCV-DTDECSIGNPCGN----------GTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICE--NGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGT 2430          

HSP 14 Score: 83.1889 bits (204), Expect = 2.118e-14
Identity = 97/323 (30.03%), Postives = 135/323 (41.80%), Query Frame = 3
Query: 2568 DVNCGKGYQKIGD-NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGYEYVED-------------KGCI----------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPK 3419
            + NC +G++     NC DINEC         RC+NT GS+ C C         +  C   ++EC+   + +  C++        C +  G   C+CP G       EGC+D NEC   P + +   C N  GS+ C C +GFQ S         EC D++           IC      +N++    C C  G  +                K C           DIDEC+    LC   N  C NT GSF+C C VG      G  C+D+DEC Q+   P    C N+ G + C+C +G+V   D K C   K L+  Q K
Sbjct: 2236 ECNCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPIGYALREDQKMCK-DLDECA---EGLHDCESRG----MMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSG-----TECLDNRQGLCFAEVLQTICQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYTTDGRDIDECKVMPNLCT--NGQCINTMGSFRCFCKVGYTTDISGTSCIDLDECSQSPK-PCNYICKNTEGSYQCSCPRGYVLQEDGKTC---KDLDECQTK 2539          

HSP 15 Score: 82.8037 bits (203), Expect = 3.450e-14
Identity = 92/298 (30.87%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2604 DNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDI-------DECQ--------ASKKLCQ-GDNVV------------CQNTPG------------SFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            ++C+DI+EC S       N RC N  GSF C C+   E         P  + C    + +++  +    SP  C +  G   C+CP G  Y++K E CIDINEC+ +P  CL   +C N+ G F C CP GF  S +        C D++       C     + +C+    +   + K C          D C+        A + LC  G   V            C  +PG            SF+C CP+G  L+Y    CVD DEC   N C  G TC N  G F C CN+GF
Sbjct: 1969 NDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYEL-------TPDGKNCIDTNECVALPGSC---SPGTCQNLEGSFRCICPPG--YEVKSENCIDINECDEDPNICL-FGSCTNTPGGFQCLCPPGFVLSDNG-----RRCFDTR----QSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCVDTDECSIGNPCGNG-TCTNVIGSFECNCNEGF 2243          

HSP 16 Score: 80.4925 bits (197), Expect = 1.478e-13
Identity = 51/125 (40.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 3015 IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID C+       NG C   + SYRC C+ GY+   +  CID+DEC  S     GD   C NTPGS+ C C  G +    ++ C+DIDEC QN    +   C+N+ G F C CN GF    D K C
Sbjct:  495 IDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDEC-TSNPCTNGD---CVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNC 615          

HSP 17 Score: 80.4925 bits (197), Expect = 1.621e-13
Identity = 105/344 (30.52%), Postives = 139/344 (40.41%), Query Frame = 3
Query: 2409 LTEINLLKDEGHRLT-----FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCID--INECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY--EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +     L +EG+ LT      I  N  V +P S        +    Q+L   F   C       GY+   +NCIDINECD     C   S C NTPG F C C        P F  +     C   R+     +             NG   C  P+ F     + C      E   +PC  +   ++   +F   CP G     S+H    D++EC +S     NG C N  GS+RC C  GY  +Y   + C+D DEC          N  C N  GSF+C C  G E      C DI+EC QN        C+N+FG + CTC  G+    D K C +LD+
Sbjct: 1994 IGSFKCLCNEGYELTPDGKNCIDTNECVALPGSC-------SPGTCQNLEGSFRCICP-----PGYEVKSENCIDINECDEDPNICLFGS-CTNTPGGFQCLCP-------PGFVLSDNGRRCFDTRQSFCFTNF-----------ENGK--CSVPKAFNTTKAKCCCSKMPGEGWGDPC--ELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVR-CVDTDECSIGNPC---GNGTCTNVIGSFECNCNEGFEPGPMMNCEDINECAQNPL-LCAFRCMNTFGSYECTCPIGYALREDQKMCKDLDE 2297          

HSP 18 Score: 79.337 bits (194), Expect = 3.178e-13
Identity = 90/308 (29.22%), Postives = 126/308 (40.91%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSG--NGIC----QNMIGSYRCACHNGYEYVEDKGCI-------------------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q+      CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   C+C  GF        C D++EC          C NS GSF C CP G    +  G V +D    S    G   G+C       +    C C N  +Y   + C                          DI+EC     +C   N +C+N  GS++C C  G E    G  C+DIDEC  N    +   C N+ G ++CTC  G+VF  +T+ CE
Sbjct:  560 CHAGFQRTPTKQACIDIDECIQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKNCVDH-DECTTTNMCLN----------GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICMNGHCINSEGSFRCDCPPGLAVGMD-GRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCAN-PDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDGRDINECALDPDICA--NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCE 851          

HSP 19 Score: 77.411 bits (189), Expect = 1.557e-12
Identity = 95/310 (30.65%), Postives = 133/310 (42.90%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTL 3401
            C  GY Q    +CID++EC S  C  N  C+NTPGS+ C C    +    +  C   I+EC        I + V C++   C +++G   C+C  GF  T D K  C+D +EC   N CL+   C N  GSF C C  GF  + +   C D+DEC+   IC  NG C N  GS+RC C  G                   Y  ++   C+       +K  C       G    CQ  P      F  +C  G+     G  DI+EC  +        C N  G + C CN G+  +   + C ++D+ L
Sbjct:  521 CNMGYKQDANGDCIDVDECTSNPC-TNGDCVNTPGSYYCKCHAGFQRTPTKQACI-DIDEC--------IQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKN-CVDHDECTTTNMCLN-GMCINEDGSFKCICKPGFVLAPNGRYCTDVDECQTPGICM-NGHCINSEGSFRCDCPPGLAVGMDGRVCVDTHMRSTCYGGIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNSAEFHGLCSSGVGITVDG-RDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECL 815          

HSP 20 Score: 73.1738 bits (178), Expect = 2.717e-11
Identity = 106/366 (28.96%), Postives = 148/366 (40.44%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGC------------APKIE---ECSFERKI---ISICDAVTCRSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-----------------QASKKLC-------QGDNVVCQN--TPGS--FKCVCP----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            +L  +F   C D   G    + G NC DI+EC D + C N   C+NTPG + C C  + + LNP   GC             P+ +    C+ E  +    S C     ++  N    C   N  E+  +CP G  +      I   DI+EC   P L     C N++GSF C CP+G+  S     C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +                    +K++C       +  N  C+   TPG+  FK +C        +      VDIDEC++         CIN  G F C C  GF +N+    C    E     N+ Q  AD +
Sbjct: 1508 NLPGMFHCICDD---GYELDRTGGNCTDIDECADPINCVN-GLCVNTPGRYECNCPPDFQ-LNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEIGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEDTRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCI 1868          

HSP 21 Score: 72.7886 bits (177), Expect = 3.647e-11
Identity = 71/233 (30.47%), Postives = 95/233 (40.77%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC S    C     C NTPGSF C C            C   ++EC    +    C  +            G   C CP+G+    +   C+D NEC+       A+C N+ GS+ C CP GF +      C D++EC  SK     G C N  G Y C C  GY  V    C+
Sbjct: 2518 PRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCK---CPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDATGLNCE-DVDECDGNHRCQHGCQNIL-----------GGYRCGCPQGYIQHYQWNQCVDENECSNPNACGSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCNYG-CSNTEGGYLCGCPPGYYRVGQGHCV 2734          

HSP 22 Score: 70.8626 bits (172), Expect = 1.331e-10
Identity = 84/262 (32.06%), Postives = 115/262 (43.89%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASK-KLCQGDNVVCQNTPGSFKCVCPVGL-EYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC  D   C N   C N  GS+ C C++  E       C   I+EC   R +   CD   CR+      + G   C CP G+ +  + E C DINEC  NPC++ A CRN+ GSF+C C  G + S   G + ID        S  G C   I   RC  +     ++ + C  +     S  + C+ D              CP GL   K   C D++EC+     CP G  C+NS G F+C C +G   +   + C
Sbjct:  768 DINECALDPDICAN-GICENLRGSYRCNCNSGYEPDASGRNCI-DIDECLVNRLL---CDNGLCRN------TPGSYSCTCPPGYVFRTETETCEDINECESNPCVNGA-CRNNLGSFNCECSPGSKLS-STGLICID--------SLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTA------------CPRGLARIKGVTCEDVNECEVFPGVCPNGR-CVNSKGSFHCECPEGLTLDGTGRVC 995          

HSP 23 Score: 61.6178 bits (148), Expect = 9.072e-8
Identity = 39/97 (40.21%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            +    C D+DECQA   +CQG N  C NT GSF+C CP G +  E  + C DIDEC       E   C N+ G + C C +G+V + D   C   +T
Sbjct:  269 IRTGACQDVDECQAIPGICQGGN--CINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCIDQRT 363          

HSP 24 Score: 55.4546 bits (132), Expect = 5.770e-6
Identity = 87/330 (26.36%), Postives = 124/330 (37.58%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC----DAVTCR-SPANCSSSNGVEFCVCPRGFTYDLKEGCIDIN------ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDK------GCIDIDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  GY+    G NCIDI+EC  + L C +N  C NTPGS+ C C     +      C   I EC     +   C     +  C  SP +  SS G+           D  +G   +N      E N+N    K+ C  + G+           D  CP+G        C D++EC        NG C N  GS+ C C  G                    ++ ED+      G   +D C  +     G        PG+  ++ +CP G  +  +G V        DI+EC+          C N+ G F C CN GF  + + + C
Sbjct:  794 NCNSGYEPDASGRNCIDIDECLVNRLLC-DNGLCRNTPGSYSCTCPPGYVFRTETETCE-DINECESNPCVNGACRNNLGSFNCECSPGSKLSSTGL--------ICIDSLKGTCWLNIQDSRCEVNINGATLKSECCATLGAAWGSPCERCELDTACPRGLARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEQCYLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGAGFANRGDVLTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNC 1113          

HSP 25 Score: 55.0694 bits (131), Expect = 8.352e-6
Identity = 40/100 (40.00%), Postives = 50/100 (50.00%), Query Frame = 3
Query: 2850 CPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CID 3137
            C RGF  +++ G C D++EC   P +     C N+ GSF+C CP G + S     C DIDEC         G C N +GSY C C  GY    D   CID
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGICQGGNCINTVGSFECRCPAGHKQSETTQKCEDIDECSIIPGICETGECSNTVGSYFCVCPRGYVTSTDGSRCID 360          
BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Match: sp|Q61555|FBN2_MOUSE (Fibrillin-2 OS=Mus musculus OX=10090 GN=Fbn2 PE=1 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 1.963e-33
Identity = 105/266 (39.47%), Postives = 150/266 (56.39%), Query Frame = 3
Query: 2610 CIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC   D+L C  N+ CIN+PGS+ C C+   + L+P   C  +  EC    +I ++C      S   C    G   C+C  GF     +  C+D++EC  +PC    TC+N+ GS++C C  GF+ + +  C+DIDEC     ++C  NG C N IGS++C C+ GYE   D K CID +EC A    C      CQN  GSF+C+CP G E + + C+DI+EC ++ N C  GS C N+ GGF C C  GFV +++ + C
Sbjct: 1841 CEDIDECSNGDNL-CQRNADCINSPGSYRCECAAGFK-LSPNGACVDR-NEC---LEIPNVC------SHGLCVDLQGSYQCICNNGFKASQDQTMCMDVDECERHPC-GNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCR-NGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPG--TCQNLEGSFRCICPPGYEVRSENCIDINECDEDPNICLFGS-CTNTPGGFQCICPPGFVLSDNGRRC 2089          

HSP 2 Score: 125.561 bits (314), Expect = 2.915e-27
Identity = 101/281 (35.94%), Postives = 140/281 (49.82%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH---FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC  +    +++C N+ GS+ C CS     +     CA  I+EC        ICD         C++  G   C+C  GF  + D+K  CID+NEC++NP  C+    C N+ GSF C C  G  YSV     GC D+DEC   +  C  +  C N+ GS++C+C  G+     K CID+DEC      C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N  G + C C  GF   +D++ C+
Sbjct: 1219 SCNPGYQATPDRQGCTDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCA-DIDEC---ENNPDICDG------GQCTNIPGEYRCLCYDGFMASMDMKT-CIDVNECDLNPNICM-FGECENTKGSFICHCQLG--YSVKKGTTGCTDVDECEIGAHNCDMHASCLNVPGSFKCSCREGWVGNGIK-CIDLDECANGTHQCS-INAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENTNLCENGQCLNVPGAYRCECEMGFTPASDSRSCQ 1483          

HSP 3 Score: 122.479 bits (306), Expect = 2.714e-26
Identity = 106/305 (34.75%), Postives = 143/305 (46.89%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSF---ERKIISIC------DAVTCRSPA-------NCSSSNGVEF-CVCPRGFTYDLKEGCI-------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ +I G  C D+NEC+       N RC+N+ GSF C C            C   ++E C     E + I         DA  C   A        C      E+  +CPRG  +  +   +       DINEC   P +     CRN+ GSF C C  GF   +    C DIDECR S    G+GIC N  GS+ C C  GYE  ++  K C+DIDEC+ +  LC+G    C NT GSF+C CP+G E     + CVDI+EC  ++       C+N  G + C+CN G+    D + C
Sbjct:  931 DAACPRGFARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEHCFLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGPGFANRGDILTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNCTDIDECRISPDLCGSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGG--TCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGC 1233          

HSP 4 Score: 122.094 bits (305), Expect = 3.965e-26
Identity = 97/264 (36.74%), Postives = 129/264 (48.86%), Query Frame = 3
Query: 2616 DINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC +        +C NT GSF C C+N       E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  TC N+ GSF C CP G + S     CVDI+EC  S     NG C NMIG+Y+C+C+ GY+   D+ GC DIDEC      C   +  C N+ GS++C C  G      G  C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct: 1066 DINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNCT-DIDECRISPDLC---------GSGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCVDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCTDIDECMIMNGGC---DTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTC 1316          

HSP 5 Score: 114.775 bits (286), Expect = 6.406e-24
Identity = 98/295 (33.22%), Postives = 133/295 (45.08%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G   +  G+ C+D NEC +      N RC+N  GS+ C C+   +  +    C    +   F   + ++C   +       +S   C    G     E C          +CP G  Y       DI+EC + P L     C N+ GSF CFC  G+   +    CVD+DEC  S K C  N IC+N  GSY+C+C  GY   ED K C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  Q + C     C N+ G F+C C +GF  +     CE
Sbjct: 2319 GMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYATDG--RDIDECKVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISGTACVDLDECSQSPKPC--NFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQ---FLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCE 2606          

HSP 6 Score: 112.079 bits (279), Expect = 4.293e-23
Identity = 98/320 (30.63%), Postives = 148/320 (46.25%), Query Frame = 3
Query: 2604 DNCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNN-----------DTKFCELDKTLNVVQP-KADLVFGIDTSESMKNYMDIVKDMLKNI 3500
            ++C+DINEC   D+L    N +C+N  G++ C C+   +      GC   I+EC     +   CD         C++S G   C C  G+   + +G  C DI+EC  NP + D   C N  G + C C  GF  S+    C+D++EC  +      G C+N  GS+ C C  GY   +   GC D+DEC+     C   +  C N PGSFKC C  G       C+D+DEC    + C   + C+N+ G + C C++GF  +            +T  CE  + LNV    + +   G   +   ++  DI +   +NI
Sbjct: 1189 EDCVDINECSLSDNLC--RNGKCVNMIGTYQCSCNPGYQATPDRQGCT-DIDECMI---MNGGCDT-------QCTNSEGSYECSCSEGYAL-MPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTCIDVNECDLNPNICMFGECENTKGSFICHCQLGYSVKKGTTGCTDVDECEIGAHNCD-MHASCLNVPGSFKCSCREGWVGNGIKCIDLDECANGTHQCSINAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENTNLCENGQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNI 1493          

HSP 7 Score: 112.079 bits (279), Expect = 4.670e-23
Identity = 74/184 (40.22%), Postives = 93/184 (50.54%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D    CID++EC  NPC     C N+ GS+ C C  GFQ +     C+DIDEC  + +   NG C N  GS++C C+ G+E   D K C+D DEC  +     G   +C N  GSFKCVC  G      G  C D+DECQ    C  G  CIN+ G F C C  G     D + C
Sbjct:  512 CECNMGYKQDANGDCIDVDECTSNPC-SNGDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNCVDHDECTTTNMCLNG---MCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICMNGH-CINNEGSFRCDCPPGLAVGVDGRVC 690          

HSP 8 Score: 110.923 bits (276), Expect = 9.713e-23
Identity = 91/277 (32.85%), Postives = 124/277 (44.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DI+EC  + +   N +C+NT GSF C+C            C   ++ECS   K            P N  C ++ G   C CPRG+   +  + C D++EC       +  C N+ G F C CP GF    H  C+D +EC     +C   GICQN  GS+ C C  G+        C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC     C   S C N+ G + C C  GF F+  +  C 
Sbjct: 2433 GPGYATDGR---DIDECKVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISGTACV-DLDECSQSPK------------PCNFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCEDVDECDGNHRCQHG----CQNILGGYRCGCPQGYVQHYQWNQCVDENECSNPGACGSAS-CYNTLGSYKCACPSGFSFDQFSSACH 2687          

HSP 9 Score: 105.145 bits (261), Expect = 5.903e-21
Identity = 89/260 (34.23%), Postives = 122/260 (46.92%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGF 3356
            G  C+D++EC       N  C NT GS+ C C     Y+  E G   K ++EC  ++                C ++ G   C CP GFT      CID NEC   P  C  K  C+N+ GSF C C +GF        C D+DEC  +  C     CQN++G YRC C  GY ++ +   C+D +EC          +  C NT GS+KC CP G  + +    C D++EC  + N C  G  C N+ GG+ C C  G+
Sbjct: 2479 GTACVDLDECSQSPKPCNFICKNTKGSYQCSCPRG--YVLQEDGKTCKDLDECQTKQHNCQFL----------CVNTLGGFTCKCPPGFTQH-HTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCEDVDECDGNHRCQHG--CQNILGGYRCGCPQGYVQHYQWNQCVDENECSNPGAC---GSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCSYG--CSNTEGGYLCGCPPGY 2718          

HSP 10 Score: 98.5969 bits (244), Expect = 5.599e-19
Identity = 98/317 (30.91%), Postives = 139/317 (43.85%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLK--------------------------EGCIDINEC-----------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G++    G NC+D +EC +     N  CIN  GSF C C        P F  AP    C+         D   C++P      +C ++ G   C CP G    +                            G +  +EC              PC  K +       F   C  G   +V     DI+EC  D  IC+ NGIC+N+ GSYRC C++GYE     + CIDIDEC  ++ LC  DN +C+NTPGS+ C CP G  ++   + C D++EC ++N C  G+ C N+ G F+C C+ G   ++    C
Sbjct:  595 CNAGFELTTDGKNCVDHDECTTTNMCLNGMCINEDGSFKCVCK-------PGFILAPNGRYCT---------DVDECQTPGICMNGHCINNEGSFRCDCPPGLAVGVDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNS-----AEFHGLCSSGIGITVDG--RDINECALDPDICA-NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLC--DNGLCRNTPGSYSCTCPPGYVFRTETETCEDVNEC-ESNPCVNGA-CRNNLGSFHCECSPGSKLSSTGLIC 883          

HSP 11 Score: 98.2117 bits (243), Expect = 5.840e-19
Identity = 66/185 (35.68%), Postives = 101/185 (54.59%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GFT+D+  G  +DI+EC   P +     C N  GSF C CP GF Y+ +   C DIDEC +   +C  N  C N  GSYRC C  G++   +  C+D +EC     +C   + +C +  GS++C+C  G +  +    C+D+DEC+++  C  G TC N+ G +NC C  GF   ++    ++D+
Sbjct: 1789 GFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCS--HGLCVDLQGSYQCICNNGFKASQDQTMCMDVDECERH-PCGNG-TCKNTVGSYNCLCYPGFELTHNNDCLDIDE 1969          

HSP 12 Score: 94.3597 bits (233), Expect = 1.003e-17
Identity = 95/297 (31.99%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCP----------------RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF------CP--------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L        CINT GSF C C     YL+ E      I+EC     +           P  C ++ G   C+CP                + F Y    G    NE   N  + K  C  +Y     +      CP               GF + +H G  VDIDEC++      NG+C N IGS+RC C  G+ Y +    C DIDEC     LCQ  N  C N+PGS++C C  G +    G CVD +EC +  N C  G  C++  G + C CN GF  + D   C
Sbjct: 1643 DIDECQELPGLCQGGNCINTFGSFQCECPQGY-YLSEETRICEDIDECFAHPGVCG---------PGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFN--VTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQ-RNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHG-LCVDLQGSYQCICNNGFKASQDQTMC 1925          

HSP 13 Score: 92.4337 bits (228), Expect = 3.369e-17
Identity = 92/311 (29.58%), Postives = 136/311 (43.73%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID--IDEC--------------------QASKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C +G+   G  CID++EC   +  C  N++C+NTPGS+ C CS  E +    F C+  ++EC+   +  ++C+         C +  G   C C  GFT       C DI+EC+        TC N  G F C C  G++     G C DIDEC D   C  NG+C N  G Y C C   ++      GC+D  +  C                      S+  C     +     C+  P      +  +CP G  ++         DIDECQ+     +G  CIN+FG F C C +G+  + +T+ CE
Sbjct: 1387 SCREGWVGNGIKCIDLDECANGTHQCSINAQCVNTPGSYRCACS--EGFTGDGFTCS-DVDECA---ENTNLCEN------GQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYELDRTGGNCTDIDECADPINCV-NGLCVNTPGRYECNCPPDFQLNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEVGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEETRICE 1684          

HSP 14 Score: 88.5817 bits (218), Expect = 5.595e-16
Identity = 86/273 (31.50%), Postives = 121/273 (44.32%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNG-ICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            D+NEC +S    +N +CINT GSF C C            C    +ECS    I + C   TC      ++  G   C C  GF       C DINEC  NP L    C N++GS++C CP G+        C D+DEC +    C   G +C+N+IG++ C C  G     D +GC+D +EC+    +C+  N  C N  GS++C C  G +    G   +D  Q           C   S+  N      C C+ G  + +  + C L  T
Sbjct: 2164 DVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCV-DTDECS----IGNPCGNGTC------TNVIGSFECTCNEGFEPGPMMNCEDINECAQNPLLCAFRCMNTFGSYECTCPVGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICE--NGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGT 2423          

HSP 15 Score: 83.9593 bits (206), Expect = 1.311e-14
Identity = 91/297 (30.64%), Postives = 124/297 (41.75%), Query Frame = 3
Query: 2604 DNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDI-------DECQ--------ASKKLCQ-GDNVV------------CQNTPG------------SFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            ++C+DI+EC S       N RC N  GSF C C+   E         P  + C    + +++  +    SP  C +  G   C+CP G+     E CIDINEC+ +P  CL   +C N+ G F C CP GF  S +        C D++       C     + +C+    +   + K C          D C+        A + LC  G   V            C  +PG            SF+C CP+G  L+Y    CVD DEC   N C  G TC N  G F CTCN+GF
Sbjct: 1962 NDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYEL-------TPDGKNCIDTNECVALPGSC---SPGTCQNLEGSFRCICPPGYEV-RSENCIDINECDEDPNICL-FGSCTNTPGGFQCICPPGFVLSDNG-----RRCFDTR----QSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVRCVDTDECSIGNPCGNG-TCTNVIGSFECTCNEGF 2236          

HSP 16 Score: 82.4185 bits (202), Expect = 4.292e-14
Identity = 94/309 (30.42%), Postives = 128/309 (41.42%), Query Frame = 3
Query: 2607 NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-----------ICSGNGICQNMIGSYRCACHNGYEYVED-------------KGCI----------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPK 3419
            NC DINEC         RC+NT GS+ C C         +  C   ++EC+   + +  C++        C +  G   C+CP G       EGC+D NEC   P + +   C N  GS+ C C +GFQ S         EC D++           +C      +N++    C C  G  +                K C           DIDEC+    LC   N  C NT GSF+C C VG      G  CVD+DEC Q+   P    C N+ G + C+C +G+V   D K C   K L+  Q K
Sbjct: 2243 NCEDINECAQNPLLCAFRCMNTFGSYECTCPVGYALREDQKMCK-DLDECA---EGLHDCESRG----MMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSG-----TECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYATDGRDIDECKVMPSLCT--NGQCVNTMGSFRCFCKVGYTTDISGTACVDLDECSQSPK-PCNFICKNTKGSYQCSCPRGYVLQEDGKTC---KDLDECQTK 2532          

HSP 17 Score: 81.6481 bits (200), Expect = 7.292e-14
Identity = 105/344 (30.52%), Postives = 139/344 (40.41%), Query Frame = 3
Query: 2409 LTEINLLKDEGHRLT-----FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCID--INECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY--EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            +     L +EG+ LT      I  N  V +P S        +    Q+L   F   C       GY+   +NCIDINECD     C   S C NTPG F C C        P F  +     C   R+     +             NG   C  P+ F     + C      E   +PC  +   ++   +F   CP G     S+H    D++EC +S     NG C N  GS+RC C  GY  +Y   + C+D DEC          N  C N  GSF+C C  G E      C DI+EC QN        C+N+FG + CTC  G+    D K C +LD+
Sbjct: 1987 IGSFKCLCNEGYELTPDGKNCIDTNECVALPGSC-------SPGTCQNLEGSFRCICP-----PGYEVRSENCIDINECDEDPNICLFGS-CTNTPGGFQCICP-------PGFVLSDNGRRCFDTRQSFCFTNF-----------ENGK--CSVPKAFNTTKAKCCCSKMPGEGWGDPC--ELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYTGVR-CVDTDECSIGNPC---GNGTCTNVIGSFECTCNEGFEPGPMMNCEDINECAQNPL-LCAFRCMNTFGSYECTCPVGYALREDQKMCKDLDE 2290          

HSP 18 Score: 80.4925 bits (197), Expect = 1.516e-13
Identity = 51/125 (40.80%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 3015 IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID C+       NG C   + SYRC C+ GY+   +  CID+DEC  S     GD   C NTPGS+ C C  G +    ++ C+DIDEC QN    +   C+N+ G F C CN GF    D K C
Sbjct:  488 IDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDEC-TSNPCSNGD---CVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGKNC 608          

HSP 19 Score: 79.337 bits (194), Expect = 4.020e-13
Identity = 91/312 (29.17%), Postives = 127/312 (40.71%), Query Frame = 3
Query: 2577 CGKGYQKIG--DNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI---CSGN---GIC----QNMIGSYRCACHNGYEYVEDKGCI-------------------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q+      CIDI+EC  + + C N  RC+NT GSF C C+   E       C    +EC+     ++            C + +G   CVC  GF        C D++EC          C N+ GSF C CP G         VD   C D+ +   C G    G+C       +    C C N  +Y   + C                          DI+EC     +C   N +C+N  GS++C C  G E    G  C+DIDEC  N    +   C N+ G ++CTC  G+VF  +T+ CE
Sbjct:  553 CHAGFQRTPTKQACIDIDECIQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKNCVDH-DECTTTNMCLN----------GMCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICMNGHCINNEGSFRCDCPPGLAVG-----VDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCAN-PDYGFGEPCQPCPAKNSAEFHGLCSSGIGITVDGRDINECALDPDICA--NGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCE 844          

HSP 20 Score: 78.1814 bits (191), Expect = 8.789e-13
Identity = 97/310 (31.29%), Postives = 137/310 (44.19%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDI---DEC--QASKKLC-------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTL 3401
            C  GY Q    +CID++EC S  C +N  C+NTPGS+ C C    +    +  C   I+EC        I + V C++   C +++G   C+C  GF  T D K  C+D +EC   N CL+   C N  GSF C C  GF  + +   C D+DEC+   IC  NG C N  GS+RC C  G    V+ + C+D      C  +  K +C                    G    CQ  P      F  +C  G+     G  DI+EC  +        C N  G + C CN G+  +   + C ++D+ L
Sbjct:  514 CNMGYKQDANGDCIDVDECTSNPC-SNGDCVNTPGSYYCKCHAGFQRTPTKQACI-DIDEC--------IQNGVLCKN-GRCVNTDGSFQCICNAGFELTTDGKN-CVDHDECTTTNMCLN-GMCINEDGSFKCVCKPGFILAPNGRYCTDVDECQTPGICM-NGHCINNEGSFRCDCPPGLAVGVDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKSECCCANPDYGFGEPCQPCPAKNSAEFHGLCSSGIGITVDG-RDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECL 808          

HSP 21 Score: 73.559 bits (179), Expect = 1.814e-11
Identity = 106/366 (28.96%), Postives = 148/366 (40.44%), Query Frame = 3
Query: 2535 HLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGC------------APKIE---ECSFERKI---ISICDAVTCRSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-----------------QASKKLC-------QGDNVVCQN--TPGS--FKCVCP----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV 3431
            +L  +F   C D   G    + G NC DI+EC D + C N   C+NTPG + C C  + + LNP   GC             P+ +    C+ E  +    S C     ++  N    C   N  E+  +CP G  +      I   DI+EC   P L     C N++GSF C CP+G+  S     C DIDEC       G G C N +G+Y C C   Y  V     C+D+ +                    +K++C       +  N  C+   TPG+  FK +C        +      VDIDEC++         CIN  G F C C  GF +N+    C    E     N+ Q  AD +
Sbjct: 1501 NLPGMFHCICDD---GYELDRTGGNCTDIDECADPINCVN-GLCVNTPGRYECNCPPDFQ-LNPTGVGCVDNRVGNCYLKFGPRGDGSLSCNTEVGVGVSRSSCCCSLGKAWGNPCETCPPVNSTEYYTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEETRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCI 1861          

HSP 22 Score: 73.559 bits (179), Expect = 2.129e-11
Identity = 83/301 (27.57%), Postives = 117/301 (38.87%), Query Frame = 3
Query: 2304 KIVLSQCNGAR----LGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINN----DVDIPRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            K++ S C   +    +GS R    VG  T+++         +++          FI  N         PR  + Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC S    C     C NTPGSF C C            C   ++EC    +    C  +            G   C CP+G+    +   C+D NEC+       A+C N+ GS+ C CP GF +      C D++EC  SK     G C N  G Y C C  GY  V    C+
Sbjct: 2447 KVMPSLCTNGQCVNTMGSFRCFCKVGYTTDISG----TACVDLDECSQSPKPCNFICKNTKGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCK---CPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCE-DVDECDGNHRCQHGCQNIL-----------GGYRCGCPQGYVQHYQWNQCVDENECSNPGACGSASCYNTLGSYKCACPSGFSFDQFSSACHDVNECSSSKNPCSYG-CSNTEGGYLCGCPPGYFRVGQGHCV 2727          

HSP 23 Score: 69.3218 bits (168), Expect = 4.393e-10
Identity = 68/216 (31.48%), Postives = 95/216 (43.98%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGS----------------------FKCVCPVGL-EYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +  G   C C  G+  D     CIDI+EC +N  L D   CRN+ GS+ C CP G+ +      C D++EC +S  C  NG C+N +GS+ C C  G + +   G I ID  + +  L   DN    N  G+                          CP G    K   C D++EC+     CP G  C+NS G F+C C +G   +   + C
Sbjct:  777 CENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCEDVNEC-ESNPCV-NGACRNNLGSFHCECSPGSK-LSSTGLICIDSLKGTCWLNIQDNRCEVNINGATLKSECCATLGAAWGSPCERCELDAACPRGFARIKGVTCEDVNECEVFPGVCPNGR-CVNSKGSFHCECPEGLTLDGTGRVC 988          

HSP 24 Score: 63.5438 bits (153), Expect = 2.118e-8
Identity = 42/100 (42.00%), Postives = 52/100 (52.00%), Query Frame = 3
Query: 3093 CHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+   +    C D+DECQA   LCQG N  C NT GSF+C CP G +  E  + C DIDEC       E   C N+ G + C C +GFV + D   C
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGLCQGGN--CINTVGSFECRCPAGHKQSETTQKCEDIDECSVIPGVCETGDCSNTVGSYFCLCPRGFVTSTDGSRC 358          

HSP 25 Score: 56.9954 bits (136), Expect = 2.069e-6
Identity = 89/333 (26.73%), Postives = 130/333 (39.04%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD---------LKEGC---IDINEC--NMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDK------GCIDIDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  GY+    G NCIDI+EC  + L C +N  C NTPGS+ C C        P +    + E C    + ++ C++  C + A C ++ G   C C  G             LK  C   I  N C  N+N    K+ C  + G+           D  CP+GF       C D++EC        NG C N  GS+ C C  G                    ++ ED+      G   +D C  +     G        PG+  ++ +CP G  +  +G +        DI+EC+          C N+ G F C CN GF  + + + C
Sbjct:  787 NCNSGYEPDASGRNCIDIDECLVNRLLC-DNGLCRNTPGSYSCTCP-------PGYVFRTETETC----EDVNECESNPCVNGA-CRNNLGSFHCECSPGSKLSSTGLICIDSLKGTCWLNIQDNRCEVNINGATLKSECCATLGAAWGSPCERCELDAACPRGFARIKGVTCEDVNECEVFPGVCPNGRCVNSKGSFHCECPEGLTLDGTGRVCLDIRMEHCFLKWDEDECIHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGPGFANRGDILTGRPFYKDINECKAFPGMCTYGKCRNTIGSFKCRCNNGFALDMEERNC 1106          

HSP 26 Score: 55.0694 bits (131), Expect = 9.237e-6
Identity = 45/115 (39.13%), Postives = 61/115 (53.04%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQ-GDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEG 3302
            C +GF  ++  G C D+DEC+    +C G G C N +GS+ C C  G++  E  + C DIDEC     +C+ GD   C NT GS+ C+CP G      G   ID  Q+  TC  G
Sbjct:  261 CRRGFIPNIRTGACQDVDECQAIPGLCQG-GNCINTVGSFECRCPAGHKQSETTQKCEDIDECSVIPGVCETGD---CSNTVGSYFCLCPRGFVTSTDGSRCID--QRAGTCFSG 369          
BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8CG19|LTBP1_MOUSE (Latent-transforming growth factor beta-binding protein 1 OS=Mus musculus OX=10090 GN=Ltbp1 PE=1 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 2.923e-33
Identity = 108/305 (35.41%), Postives = 146/305 (47.87%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVC-------------YCSNNEEYLNPE-------------FGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            G NCID++EC       + RCINT G+F C             YC + +E L P              + C P  E   F        D   C  P      +C++  G   C C RG+  T D +  C DI+EC          CRN+ GSF C C +G+Q S     C DIDEC    +CS +G C+N  GS++C C+ GY   V    C DI+EC     +CQG +  C NT GS+ C CP G +  + KGC DI+EC Q   C     C+N+ G F+C C +GF  + D + CE +D+ +N
Sbjct:  944 GTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTE--GFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYSPTPDHRH-CQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCS-HGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGD--CINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVN 1242          

HSP 2 Score: 138.658 bits (348), Expect = 2.537e-31
Identity = 99/277 (35.74%), Postives = 135/277 (48.74%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            V C +G++     C+D++EC       N  C N  GS++C C        P+      I+EC      ++            C +++G   C C +G+     K+ C DI+EC  +       CRN+ GSF C C +G++ SV  G  C DI+EC  DS +C G G C N  GSY C C +G++  ++KGC DI+EC A   LC G +  C NT GSF CVC  G      G  C DIDEC  N  C     C N+ G F C C +GF    D + C
Sbjct: 1015 VPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRGYS-PTPDHRHCQDIDECQQGNLCMN----------GQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQCRNTEGSFQCVCNQGYRASV-LGDHCEDINECLEDSSVCQG-GDCINTAGSYDCTCPDGFQLNDNKGCQDINEC-AQPGLC-GSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGC 1276          

HSP 3 Score: 121.709 bits (304), Expect = 3.718e-26
Identity = 94/265 (35.47%), Postives = 133/265 (50.19%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTC 3344
            +C +GY    D+  C DI+EC       N +C NT GSF C C    +    +  C   I+EC        +C    CR+      + G   CVC +G+    L + C DINEC  +  +     C N+ GS+DC CP GFQ + + GC DI+EC    +C  +G C N  GS+ C C  G+    D + C DIDEC  +  +C   +  C NT GSF+C+C  G +  +  +GCVD++EC+  +  C E + C N  G F C C
Sbjct: 1055 SCHRGYSPTPDHRHCQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCE-DIDECEHHH----LCSHGQCRN------TEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDEC-VNNTVCD-SHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGE-AFCENVEGSFLCVC 1305          

HSP 4 Score: 120.553 bits (301), Expect = 8.362e-26
Identity = 98/268 (36.57%), Postives = 135/268 (50.37%), Query Frame = 3
Query: 2610 CIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFC-VCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C+DI+EC  +  H  +  RC NT GSF+C C     ++  E G  C   ++EC              CR    C ++ G   C  C  G+    +  C DI+EC       +  C N+ GS+ C  C +GF+   +  C+D+DEC   K+C+ NG C N+ GSY C+CH GY    D + C DIDECQ    LC   N  C+NT GSF+C C  G  L   +  C DIDEC+ ++ C  G  C N+ G F C CN+G+  +     CE
Sbjct:  905 CVDIDECAQVR-HLCSQGRCENTEGSFLCVCPAG--FMASEEGTNCI-DVDECLRPD---------MCRD-GRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTEGFR-GWNGQCLDVDECLQPKVCT-NGSCTNLEGSYMCSCHRGYSPTPDHRHCQDIDECQQG-NLCM--NGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHHLCSHGQ-CRNTEGSFQCVCNQGYRASVLGDHCE 1152          

HSP 5 Score: 86.2705 bits (212), Expect = 2.284e-15
Identity = 61/161 (37.89%), Postives = 87/161 (54.04%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNC-TCNKGF 3356
            +INEC +NP +     C N    + C C +G+++S     CVDIDEC   +     G C+N  GS+ C C  G+   E+   CID+DEC     +C+  +  C NT G+F+C  C  G     +G C DIDEC + +TCPE   C+N+ G + C  C +GF
Sbjct:  865 EINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCIDVDEC-LRPDMCR--DGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCPE-EQCVNTPGSYQCVPCTEGF 1021          

HSP 6 Score: 70.4774 bits (171), Expect = 1.776e-10
Identity = 96/341 (28.15%), Postives = 137/341 (40.18%), Query Frame = 3
Query: 2550 FDITCKDVNCGKGYQKIGDN--CIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFG----------CVDIDECRDSKICSGN----GICQNMIGS----YRCACHNGYEYVED---------------------KGCI---------------DIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +D TC D     G+Q + DN  C DINEC     C ++  C+NT GSF C C            C   I+EC          +   C S   C ++ G   C+C +GF      +GC+D+NEC + +    +A C N  GSF C C    Q YS   G           VD     + K C  N     +C N++        C C +G  + ++                     KG +               D DEC    +++C+  N  C NT   ++C C  G  Y   +  C D+DECQ  N+C +G  C+N+ G +NC C    V +   K C
Sbjct: 1176 YDCTCPD-----GFQ-LNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCE-DIDECV---------NNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRVTEDSGVDRQPREEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVPAGESSYDTGGENYKDADECLLFGEEICK--NGYCLNTQPGYECYCKQGTYYDPVKLQCFDMDECQDPNSCIDGQ-CVNTEGSYNCFCTHPMVLDASEKRC 1497          
BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Match: sp|Q75N90|FBN3_HUMAN (Fibrillin-3 OS=Homo sapiens OX=9606 GN=FBN3 PE=2 SV=3)

HSP 1 Score: 144.436 bits (363), Expect = 6.485e-33
Identity = 104/275 (37.82%), Postives = 145/275 (52.73%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G  Y  I   C D++EC S    C  N+ CIN PGS+ C C+   + L+P   C  +  EC   R+I ++C      S  +C  + G   C+C RGF     +  C+DI+EC+  PC    TC+N  GS++C C  GF  + +  CVD DEC     ++C   G C N  GS+ C C +G+E   D K C+D +EC +    C      CQN  GSF+C+CP G + +   C+DIDEC ++ N C  G TC NS G F C C  GFV +++   C
Sbjct: 1751 GFNYNSILLACEDVDECGSRESPCQQNADCINIPGSYRCKCTRGYK-LSPGGACVGR-NEC---REIPNVC------SHGDCMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQPC-GNGTCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCR-FGHCLNTAGSFHCLCQDGFELTADGKNCVDTNECLSLAGTCL--PGTCQNLEGSFRCICPPGFQVQSDHCIDIDECSEEPNLCLFG-TCTNSPGSFQCLCPPGFVLSDNGHRC 2009          

HSP 2 Score: 135.961 bits (341), Expect = 2.363e-30
Identity = 115/309 (37.22%), Postives = 144/309 (46.60%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNN-----------EEYLNPEFGCAPKIEECSFE-----RKIISICD-----AVTCRSPANCSSSNGVEFC-VCPRGFTY---DLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYE--YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C +G+ ++ G  C D+NEC+S      N RC+NT GSF C C              +  L P F      +EC        R  +  C       V C +   C     +EF  +CPRG  +   D   G     D+NEC + P L    TCRN+ GSF C C  GF        C DIDECR S    G G C N  GS+ C C  GYE  ++  K C+D+DEC     LC+G    C NT GS+KC CP G E   KG  C DIDEC   +  CP G  C+N  G F C+C+ GF    D + C
Sbjct:  852 DPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGLMLDASGRLCVDVRLEPCF-LRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEA---CPDPESLEFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALDAQERNCTDIDECRISPDLCGQGTCVNTPGSFECECFPGYESGFMLMKNCMDVDECARDPLLCRGG--TCTNTDGSYKCQCPPGHELTAKGTACEDIDECSLSDGLCPHGQ-CVNVIGAFQCSCHAGFQSTPDRQGC 1153          

HSP 3 Score: 118.242 bits (295), Expect = 5.044e-25
Identity = 98/314 (31.21%), Postives = 140/314 (44.59%), Query Frame = 3
Query: 2442 HRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            H   +     DVD          R    L  H   +  +     +C  GY        C+D++EC  +       C NT GSF+C C     YL  E G   K ++EC+  +                C ++ G   C CP GFT    + C D +EC+    PC     C N+ GSF C C +GF   S   GC D++EC     C     CQN +G YRC+C  G+ ++ +   C+D +EC  S   C   +  C+NT G F+CVCP G ++ +   GC ++DEC      P   +C N+ GGF C C +G+
Sbjct: 2353 HGSGYTAEGRDVD--------ECRMLAHLCAHGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKPCTFLCKNTKGSFLCSCPRG--YLLEEDGRTCKDLDECTSRQHNCQF----------LCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG--CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTC--GSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRG-PCSYSCANTPGGFLCGCPQGY 2640          

HSP 4 Score: 115.161 bits (287), Expect = 4.317e-24
Identity = 107/328 (32.62%), Postives = 150/328 (45.73%), Query Frame = 3
Query: 2439 GHRLTFI-TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLC--QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            GH LT   T   D+D            +  L  H   V  I     +C  G+Q   D   C+DINEC       +  CINT GS+ C C      +     CA  ++EC    ++   CD        +C++  G   C+C  GF  T D++  C+D++EC++NP  CL    C N+ GSF C C  G+       GC D+DEC      C  +  C N+ GS+ C C  G+  V D   C D+DEC + +  C  +GD   C N PGS++C C  G       C D DEC +N    +   C+N+ GG+ C C  GF    D + C+
Sbjct: 1101 GHELTAKGTACEDID--------ECSLSDGLCPHGQCVNVIGAFQCSCHAGFQSTPDRQGCVDINECRVQNGGCDVHCINTEGSYRCSCGQGYSLMPDGRACA-DVDECEENPRV---CDQ------GHCTNMPGGHRCLCYDGFMATPDMRT-CVDVDECDLNPHICL-HGDCENTKGSFVCHCQLGYMVRKGATGCSDVDECEVGGHNCDSHASCLNIPGSFSCRCLPGW--VGDGFECHDLDECVSQEHRCSPRGD---CLNVPGSYRCTCRQGFAGDGFFCEDRDECAENVDLCDNGQCLNAPGGYRCECEMGFDPTEDHRACQ 1403          

HSP 5 Score: 113.235 bits (282), Expect = 1.674e-23
Identity = 95/288 (32.99%), Postives = 131/288 (45.49%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G GY   G    D++EC  LA H   +  CIN+ GSF C+C            C   ++ECS   K  +            C ++ G   C CPRG+  + ++G  C D++EC       +  C N+ G+F C CP GF    H  C D DEC      C  +G C N  GS+RC CH G+  V    GC D++EC    +   G    CQN  G ++C CP G     +   CVD +EC  +      ++C N+ GGF C C  GF F+     C E+D+      P
Sbjct: 2354 GSGYTAEGR---DVDECRMLA-HLCAHGECINSLGSFRCHCQAGYTPDATATTCL-DMDECSQVPKPCTF----------LCKNTKGSFLCSCPRGYLLE-EDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCPPGFTQH-HQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECDGPHRCQHG----CQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGP 2620          

HSP 6 Score: 106.301 bits (264), Expect = 2.031e-21
Identity = 70/187 (37.43%), Postives = 99/187 (52.94%), Query Frame = 3
Query: 2817 CSSSNGVEFCVCPRGFTYDLKE-GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG---LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C +++G   C CP G++ D     C+D +EC++     + TC N  G F+C C  GF+  +   C DIDEC  +  +C+    C N  GSY C C  GY   ED   C D+DEC   ++ C    + C+N  G+F CVCP G   L    +GC D +EC  Q + C  G  C+N+ G F C C++GF
Sbjct: 2100 CVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFR--CHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGR-CVNTAGSFRCDCDEGF 2283          

HSP 7 Score: 103.605 bits (257), Expect = 1.518e-20
Identity = 73/184 (39.67%), Postives = 95/184 (51.63%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+T D++  CID++EC  +PC     C N  G++ C C  GFQ +     CVD+DEC  S      G C N  GS++C C+ G+E   D K C+D +EC A+  +C   N VC N  GSF C+C  G      G  C+DIDECQ    C  G  C N+ G F C C  G     D + C
Sbjct:  433 CECNVGYTQDVRGECIDVDECTSSPC-HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCVNTEGSFQCVCNAGFELSPDGKNCVDHNEC-ATSTMCV--NGVCLNEDGSFSCLCKPGFLLAPGGHYCMDIDECQTPGICVNGH-CTNTEGSFRCQCLGGLAVGTDGRVC 611          

HSP 8 Score: 100.908 bits (250), Expect = 9.191e-20
Identity = 76/232 (32.76%), Postives = 115/232 (49.57%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG 3254
            +C +GY  ++ G  C D++EC S   +    C+NT G+F C C        P F    +  +  F+    S      C +  +C ++ G   C C +GFT      GC D+NEC+  P   +  C+N  G + C CP+GF Q+S    CVD +EC  S    G+  C+N +G +RC C +G+++ +   GC ++DEC   +  C   +  C NTPG F C CP G     +G
Sbjct: 2430 SCPRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCRCP-------PGF---TQHHQACFDNDECSA-QPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGCEDVNECD-GPHRCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPC---SYSCANTPGGFLCGCPQGYFRAGQG 2646          

HSP 9 Score: 97.0561 bits (240), Expect = 1.428e-18
Identity = 81/236 (34.32%), Postives = 116/236 (49.15%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESM----KNYMDIVKDMLKNIITDYKLKSLA 3530
            GF  D+  G  +DI+EC   P +     C N  GSF C CP GF Y S+   C D+DEC  R+S  C  N  C N+ GSYRC C  GY+      C+  +EC+    +C  GD   C +T GS+ C+C  G +    +  C+DIDEC +   C  G TC N  G +NC C  GFV  ++    + D+   +V         ++T+ S     ++  ++  D  KN +   +  SLA
Sbjct: 1709 GFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESP-CQQNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD---CMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQ-PCGNG-TCKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFELTADG-KNCVDTNECLSLA 1937          

HSP 10 Score: 91.2781 bits (225), Expect = 7.578e-17
Identity = 97/289 (33.56%), Postives = 129/289 (44.64%), Query Frame = 3
Query: 2607 NCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF------------QYSVHFGCVDIDECRDSKI---------CS---GNG------IC-QNMIGSYRCACHNGYEYVE--DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C+D +EC +L         C+NT GSF C C +  E            + C    + +S+  A TC  P  C +  G   C+CP GF     + CIDI+EC+  P  CL   TC NS GSF C CP GF            + S  F   +  +C   K          CS   G G      +C Q    +++  C  G+  V   D    D++EC  +  +C   N VC NT GSF+C CP G  L++    CVD DEC   + C +G TC N  GGF C C  GF
Sbjct: 1883 DCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFEL-------TADGKNCVDTNECLSL--AGTCL-PGTCQNLEGSFRCICPPGFQVQ-SDHCIDIDECSEEPNLCL-FGTCTNSPGSFQCLCPPGFVLSDNGHRCFDTRQSFCFTRFEAGKCSVPKAFNTTKTRCCCSKRPGEGWGDPCELCPQEGSAAFQELCPFGHGAVPGPDDSREDVNECAENPGVCT--NGVCVNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQG-TCTNVIGGFECACADGF 2156          

HSP 11 Score: 89.3521 bits (220), Expect = 3.248e-16
Identity = 92/307 (29.97%), Postives = 127/307 (41.37%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDL-KEGCIDIN-ECNMNPCLDKATCRNSY------------------------------GSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            G NC+D NEC +     N  C+N  GSF C C        P F  AP    C          D   C++P      +C+++ G   C C  G         C+D +        ++K +C   +                                F   C  G   +      DI+EC  D ++C+ NG+C+N+ GSYRC C+ GYE     K C D+DEC  +  LC  DN  CQN+PGS+ C CP G  + +    C D+DEC  ++ C  G  C N  G + C C  G   +    FC LD T
Sbjct:  526 GKNCVDHNECATSTMCVNGVCLNEDGSFSCLC-------KPGFLLAPGGHYCM---------DIDECQTPGICVNGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG--RDINECALDPEVCA-NGVCENLRGSYRCVCNLGYEAGASGKDCTDVDECALNSLLC--DNGWCQNSPGSYSCSCPPGFHFWQDTEICKDVDEC-LSSPCVSG-VCRNLAGSYTCKCGPGSRLDPSGTFC-LDST 808          

HSP 12 Score: 88.9669 bits (219), Expect = 3.942e-16
Identity = 62/168 (36.90%), Postives = 84/168 (50.00%), Query Frame = 3
Query: 2889 IDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            IDI     N CL+   C  +  S+ C C  G+   V   C+D+DEC  S     +G C N+ G+Y C C+ G++    +  C+D+DEC  S  LC     V  NT GSF+CVC  G E    G  CVD +EC  +  C  G  C+N  G F+C C  GF+      +C
Sbjct:  409 IDICRHFTNLCLN-GRCLPTPSSYRCECNVGYTQDVRGECIDVDECTSSPC--HHGDCVNIPGTYHCRCYPGFQATPTRQACVDVDECIVSGGLCHLGRCV--NTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNG-VCLNEDGSFSCLCKPGFLLAPGGHYC 570          

HSP 13 Score: 87.4261 bits (215), Expect = 1.138e-15
Identity = 73/214 (34.11%), Postives = 98/214 (45.79%), Query Frame = 3
Query: 2616 DINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKI-CSGNGI-CQNMIGSYRCACHNGYEYV--EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            D+NEC ++     N  C+NT GSF C C            C    +ECS                   C++  G   C C  GF   L   C DI+EC++NP L    C N+ GS+ C CP G+        C D+DEC D +  C   G+ C+N+IG++ C C  G   +    +GC D +EC A   LC   N  C NT GSF+C C  G +
Sbjct: 2084 DVNECAENPGVCTNGVCVNTDGSFRCECPFGYSLDFTGINCV-DTDECSVGHPC----------GQGTCTNVIGGFECACADGFEPGLMMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCV--NGRCVNTAGSFRCDCDEGFQ 2284          

HSP 14 Score: 85.1149 bits (209), Expect = 6.437e-15
Identity = 96/341 (28.15%), Postives = 131/341 (38.42%), Query Frame = 3
Query: 2460 TINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV--HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-------------------YEYVEDKGCIDIDECQASKKLC------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            T+N  +DI R    L +       + L       C+   C  GY Q +   CID++EC S  CH+   C+N PG++ C C    +       C   ++EC     +  +           C ++ G   CVC  GF      + C+D NEC  +       C N  GSF C C  GF  +   H+ C+DIDEC+   IC  NG C N  GS+RC C  G                   Y  +E   C        +K  C       G    CQ  P      F+ +C  GL     G  DI+EC  +        C N  G + C CN G+      K C
Sbjct:  404 TLNQTIDICRHFTNLCLN-----GRCLPTPSSYRCE---CNVGYTQDVRGECIDVDECTSSPCHHGD-CVNIPGTYHCRCYPGFQATPTRQACV-DVDECIVSGGLCHL---------GRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTMCVNGVCLNEDGSFSCLCKPGFLLAPGGHY-CMDIDECQTPGICV-NGHCTNTEGSFRCQCLGGLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPAKDSAEFQALCSSGLGITTDG-RDINECALDPEVCANGVCENLRGSYRCVCNLGYEAGASGKDC 722          

HSP 15 Score: 80.8777 bits (198), Expect = 1.202e-13
Identity = 91/298 (30.54%), Postives = 126/298 (42.28%), Query Frame = 3
Query: 2616 DINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDI--------------NECNMNPCLDKATCRNSYGSFDCF------CP---------------KGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DI+EC  L   C     C+NT GSF C C     +L+        I+ECS    I           P  C ++ G   CVCP  +   +    C+D+              NE   N  + +  C  SY     +      CP                GF   +H G  +DIDEC +      NGIC N IGS+RC C  G+ Y      C D+DEC + +  CQ  N  C N PGS++C C  G +    G CV  +EC++  N C  G  C+++ G + C C++GF  + D   C
Sbjct: 1563 DIDECQELPGLCQGGD-CVNTFGSFQCECPPGY-HLSEHTRICEDIDECSTHSGICG---------PGTCYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFN--VTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACEDVDECGSRESPCQ-QNADCINIPGSYRCKCTRGYKLSPGGACVGRNECREIPNVCSHGD-CMDTEGSYMCLCHRGFQASADQTLC 1845          

HSP 16 Score: 80.8777 bits (198), Expect = 1.275e-13
Identity = 71/235 (30.21%), Postives = 102/235 (43.40%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK-----------VFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNP-CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  + +   RT K+L +  S+           V   TC+   C  G+ +    C D +EC +    C  +  C NTPGSF C C      ++   GC           + ++ CD    R    C +  G   C CP+GFT +     C+D NEC ++P     A+CRN+ G F C CP GF +    G C ++DEC   +  CS +  C N  G + C C  GY       C+
Sbjct: 2432 PRGYLLEEDGRTCKDLDECTSRQHNCQFLCVNTVGAFTCR---CPPGFTQHHQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLVSSGHGC-----------EDVNECDGPH-RCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCGSASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPCSYS--CANTPGGFLCGCPQGYFRAGQGHCV 2649          

HSP 17 Score: 80.1073 bits (196), Expect = 1.831e-13
Identity = 106/337 (31.45%), Postives = 143/337 (42.43%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERK-------------IISICDAVTC----RSPAN----CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG---CIDIDE----------CQ------ASKKLC-------QGDNVVCQNTPGS----FKCVC----PVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+  + G NC DINEC D + C N   CINTPGS++C C  + E LNP   GC   +   C  E                + +  A  C    R+  N    C  +N  E+  +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  S H   C DIDEC       G G C N +G+Y C C    EY++  G   C+D+ +          CQ       ++K+C       Q  N  C+  P      ++ +C    P  L     G  +DIDEC +         CIN  G F C C  GF +N+    CE
Sbjct: 1430 CNGGYELDRGGGNCTDINECADPVNCINGV-CINTPGSYLCSCPQDFE-LNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICEDIDECSTHSGICGPGTCYNTLGNYTCVCP--AEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNIGQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQIGSFRCECPAGFNYNSILLACE 1762          

HSP 18 Score: 77.7962 bits (190), Expect = 9.598e-13
Identity = 84/310 (27.10%), Postives = 126/310 (40.65%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID---------------------------IDECQASKKLCQGDNVVCQNTPGS----FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G  C D++EC S    C     C+N PGS+ C C   + +    F C  + +EC+   + + +CD         C ++ G   C C  GF   +    C D++EC         +C N  G F C C  G++     G C DI+EC D   C  NG+C N  GSY C+C   +E      GC+D                           +        L +     C+  P +    ++ +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  +  T+ CE
Sbjct: 1308 CLPGWVGDGFECHDLDECVSQEHRCSPRGDCLNVPGSYRCTC--RQGFAGDGFFCEDR-DECA---ENVDLCDN------GQCLNAPGGYRCECEMGFDPTEDHRACQDVDECAQGNLCAFGSCENLPGMFRCICNGGYELDRGGGNCTDINECADPVNCI-NGVCINTPGSYLCSCPQDFELNPSGVGCVDTRAGNCFLETHDRGDSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVILEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICE 1604          

HSP 19 Score: 72.0182 bits (175), Expect = 6.065e-11
Identity = 86/317 (27.13%), Postives = 128/317 (40.38%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEE---YLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINEC--NMNPCLDKAT-CRNSYGSFDCFCPKGFQ--YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQN-----------------------------TPG--SFKCVCPVGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            G NC+D +EC          C N  G F C C++  E    +  E      I+ECS    + +            C ++ G   C CP G+T  L+E    C D++EC      C  +   C+N  G+F C CP G +       GC D +EC        NG C N  GS+RC C  G++       C DI +     ++ Q    +C++                              PG  +++ +CP G  Y  +G  D+DEC+   + C  G  CINS G F C C  G+  +     C ++D+   V +P
Sbjct: 2121 GINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGLMMTCE-----DIDECSLNPLLCAF----------RCHNTEGSYLCTCPAGYT--LREDGAMCRDVDECADGQQDCHARGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQ---TMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPGTSAYRKLCPHGSGYTAEG-RDVDECRMLAHLCAHGE-CINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPKP 2415          

HSP 20 Score: 65.4698 bits (158), Expect = 5.499e-9
Identity = 89/305 (29.18%), Postives = 126/305 (41.31%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY--DLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDE-----------CRD------------------------SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDK 3395
            C DI+EC         RC NT GS++C C      L  +      ++EC+  ++    C A        C +  G   CVCP G        EGC D NEC+  P  C++   C N+ GSF C C +GFQ S     C DI +           CR                          ++C   G       +YR  C +G  Y  +    D+DEC+    LC   +  C N+ GSF+C C  G   +     C+D+DEC Q    P    C N+ G F C+C +G++   D + C +LD+
Sbjct: 2164 CEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYT-LREDGAMCRDVDECADGQQD---CHARG----MECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVN-GRCVNTAGSFRCDCDEGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCPLPG-----TSAYRKLCPHGSGYTAEG--RDVDECRMLAHLCA--HGECINSLGSFRCHCQAGYTPDATATTCLDMDECSQVPK-PCTFLCKNTKGSFLCSCPRGYLLEEDGRTCKDLDE 2449          
BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Match: sp|Q00918|LTBP1_RAT (Latent-transforming growth factor beta-binding protein 1 OS=Rattus norvegicus OX=10116 GN=Ltbp1 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.241e-32
Identity = 106/305 (34.75%), Postives = 147/305 (48.20%), Query Frame = 3
Query: 2601 GDNCIDINECDSLACHNNSRCINTPGSFVC-------------YCSNNEEYLNPE-------------FGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            G NCID++EC       + RCINT G+F C             +C + +E L P              + C P  E   F        D   C  P      +C++  G   C C +G+  T D +  C DI+EC          C+N+ GSF C C +G+Q S     C DIDEC    +CS +G C+N  GS++C C+ GY   V    C DI+EC     +CQG +  C NT GS+ C CP GL+  + KGC DI+EC Q   C     C+N+ G F+C C +GF  + D + CE +D+ +N
Sbjct:  944 GSNCIDVDECLRPDVCRDGRCINTAGAFRCEYCDSGYRMSRRGHCEDIDECLTPSTCPEEQCVNSPGSYQCVPCTE--GFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHKGYSPTPDHRH-CQDIDECQQGNLCMNGQCKNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHRHLCS-HGQCRNTEGSFQCLCNQGYRASVLGDHCEDINECLEDSSVCQGGD--CINTAGSYDCTCPDGLQLNDNKGCQDINECAQPGLCAPHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVN 1242          

HSP 2 Score: 135.576 bits (340), Expect = 2.423e-30
Identity = 98/277 (35.38%), Postives = 132/277 (47.65%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            V C +G++     C+D++EC       N  C N  GS++C C        P+      I+EC      ++            C +++G   C C +G+     K+ C DI+EC          CRN+ GSF C C +G++ SV  G  C DI+EC  DS +C G G C N  GSY C C +G +  ++KGC DI+EC A   LC   +  C NT GSF CVC  G      G  C DIDEC  N  C     C N+ G F C C +GF    D + C
Sbjct: 1015 VPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHKGYS-PTPDHRHCQDIDECQQGNLCMN----------GQCKNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHRHLCSHGQCRNTEGSFQCLCNQGYRASV-LGDHCEDINECLEDSSVCQG-GDCINTAGSYDCTCPDGLQLNDNKGCQDINEC-AQPGLC-APHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGC 1276          

HSP 3 Score: 122.865 bits (307), Expect = 1.739e-26
Identity = 101/268 (37.69%), Postives = 135/268 (50.37%), Query Frame = 3
Query: 2610 CIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFG--CAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFC-VCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            CIDI+EC + A H  +  RC NT GSF+C C     ++  E G  C   ++EC              CR    C ++ G   C  C  G+    +  C DI+EC       +  C NS GS+ C  C +GF+   +  C+D+DEC   K+C+ NG C N+ GSY C+CH GY    D + C DIDECQ    LC   N  C+NT GSF+C C  G  L   +  C DIDEC+  + C  G  C N+ G F C CN+G+  +     CE
Sbjct:  905 CIDIDEC-AQAQHLCSQGRCENTEGSFLCICPAG--FIASEEGSNCI-DVDECLRPD---------VCRD-GRCINTAGAFRCEYCDSGYRMSRRGHCEDIDECLTPSTCPEEQCVNSPGSYQCVPCTEGFR-GWNGQCLDVDECLQPKVCT-NGSCTNLEGSYMCSCHKGYSPTPDHRHCQDIDECQQG-NLCM--NGQCKNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHRHLCSHGQ-CRNTEGSFQCLCNQGYRASVLGDHCE 1152          

HSP 4 Score: 120.168 bits (300), Expect = 1.297e-25
Identity = 93/265 (35.09%), Postives = 133/265 (50.19%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTC 3344
            +C KGY    D+  C DI+EC       N +C NT GSF C C    +    +  C   I+EC        +C    CR+      + G   C+C +G+    L + C DINEC  +  +     C N+ GS+DC CP G Q + + GC DI+EC    +C+ +G C N  GS+ C C  G+    D + C DIDEC  +  +C   +  C NT GSF+C+C  G +  +  +GCVD++EC+  +  C E + C N  G F C C
Sbjct: 1055 SCHKGYSPTPDHRHCQDIDECQQGNLCMNGQCKNTDGSFRCTCGQGYQLSAAKDQCE-DIDECEHRH----LCSHGQCRN------TEGSFQCLCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGLQLNDNKGCQDINECAQPGLCAPHGECLNTQGSFHCVCEQGFSISADGRTCEDIDEC-VNNTVCD-SHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGE-AFCENVEGSFLCVC 1305          

HSP 5 Score: 85.1149 bits (209), Expect = 5.872e-15
Identity = 61/161 (37.89%), Postives = 87/161 (54.04%), Query Frame = 3
Query: 2892 DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNC-TCNKGF 3356
            +INEC +NP +     C N    + C C +G+++S     C+DIDEC  ++     G C+N  GS+ C C  G+   E+   CID+DEC     +C+  +  C NT G+F+C  C  G     +G C DIDEC   +TCPE   C+NS G + C  C +GF
Sbjct:  865 EINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQQRKCIDIDECAQAQHLCSQGRCENTEGSFLCICPAGFIASEEGSNCIDVDEC-LRPDVCR--DGRCINTAGAFRCEYCDSGYRMSRRGHCEDIDECLTPSTCPE-EQCVNSPGSYQCVPCTEGF 1021          

HSP 6 Score: 70.0922 bits (170), Expect = 2.120e-10
Identity = 102/365 (27.95%), Postives = 143/365 (39.18%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGC------------APKIE--------ECSFERKIISICDAVTCR------------SPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFG----------CVDIDECRDSKICSGN----GICQNMIGS----YRCACHNGYEYVED---------------------KGCI---------------DIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +GY+   +GD+C DINEC  DS  C     CINT GS+ C C +  + LN   GC            AP  E         C  E+      D  TC             S   C ++ G   C+C +GF      +GC+D+NEC + +    +A C N  GSF C C    Q YS   G           VD     + K C  N     +C N++        C C +G  + ++                     KG +               D DEC    +++C+  N  C NT   ++C C  G  Y   +  C D+DECQ  N+C +G  C+N+ G +NC C    V +   K C
Sbjct: 1138 CNQGYRASVLGDHCEDINECLEDSSVCQGGD-CINTAGSYDCTCPDGLQ-LNDNKGCQDINECAQPGLCAPHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRATEDSGVDRQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFSEMCPRGKGFVPAGESSYETGGENYKDADECLLFGEEICK--NGYCLNTQPGYECYCKEGTYYDPVKLQCFDMDECQDPNSCIDGQ-CVNTEGSYNCFCTHPMVLDASEKRC 1497          
BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Match: A0A267ED60 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000911g5 PE=4 SV=1)

HSP 1 Score: 507.294 bits (1305), Expect = 1.129e-147
Identity = 347/1137 (30.52%), Postives = 568/1137 (49.96%), Query Frame = 3
Query: 1701 QYCISSCPVDYDTDAQFCK-----------RRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSV----VCGVNKELQDCASSNGGECNTK--CACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITI--NNDVDIPRSIYQLSV---RTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVF-NNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYK---LKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYNSSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDK-LYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCT-LNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCA-RQVTSQCI--FD-GKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIK-DKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDN 4997
            + C+ S  VDY    + CK           RR A C SL      C+++       K+R C  +K   NSV      G  +   D          ++  CACK G+V       C+P E  C     LD+ F++DG++ V+   F  +K  +++ +T+   S  +VR  IAV+G  +PT+ L ++     D M  V ++K  PG     L  A  +  K++++   GAR    + I ++ + +++    +  + +E++ LK     +  +T+          +I  LSV   R    L+  + K+     K   C  GY++ G  CID+NEC +    CH++S C+N PGSF+C C     +       + K E+C  + K                          C  G+ +D   G C+DINEC  NPCL D ATC N+ GSF C C  GF+Y+ +FGCVDIDEC+    C+G+G C N +GS++C C  GY Y    GC DIDEC A    C+G +  C NT GS+ C C  G  Y+   C+DIDEC +++  C   +TC+N   G+ C C  G  + +   + C  D+   + Q K D +F ID S+ +K+    +KD L  ++  +K   LK L++  S   +   G +++K  + +   + + +   ++ F GGK+ L  G+K A   + +  +  S+ +V  IT +     D + +  IK      K  G+   + +    + ++W  ++  +EI  +V  +    + +I+K+IY++   LI CPEGF+ +  +CQ +T    YCK +DK L A+ STW   C+ + C  +G+ V+   C+G+DG CY  +E+FPCT + G+L+W+C+CK+   T+++ KK +       +++   AT +   L +   +CV PFK++NKW Y C +Y     WC++H  ++ G +  C  ++  ++C   F+  K+Y EG++W+ GCNV+ C         +CQ  + TC  LN D Y C   G  + +C+C+Q++ G+G+N  +  DK     +       +     D S+G+RYE G+ W   CF  +C   G  ++++  C   DGKC + G    F C + GK  ++C CA D+
Sbjct:  534 RACLKSGKVDYTKIYKSCKFDVCQYRKAKNRRKAVCESLETLYNACLAKGFRVSWRKHRFC-PLKCGANSVFRRKTSGCPRTCADLGRRRKCRLPSREGCACKEGYVL--NGKKCVP-ERICGKVVPLDLAFVVDGTDDVSSDEFETLKNLIIKLLTKYRLSSNSVRPAIAVFGRTSPTVSLFNQRPGKADFMAFVRKMKK-PGGSADNLDAALRVANKVMMNPMGGARPAVPKYILLL-KKSKVPASSESVVKSELDSLKKANVHVVAVTLPPTKPGKTDTAIGGLSVIPARVPPYLA--IPKITKELEKTAGCTSGYKRKGKRCIDVNECAAKVPPCHSDSTCVNLPGSFICLCREGFRHDRSRGCVSLKKEKCPSKPK--------------------------CDNGYRFDKANGKCVDINECKENPCLGDSATCVNTPGSFKCKCKTGFEYNKYFGCVDIDECKGKDPCAGSGKCINTLGSFKCTCDEGYTYKTGLGCKDIDECAAGSHNCKGGSK-CINTAGSYNCSCDSGYRYESNKCIDIDECAEDSKLCDASATCVNKKPGYECQCKLGSNWVHKAGRGCVPDEKKGMSQRKVDFIFTIDGSDKLKSSEKDIKDFLTRLLNKFKANELKGLSVRASAIIMGKKGGADIK--FSQADLSGIDDKIKALNFPGGKANLLDGMKKANQEHAK-ARNGSTPIVFLITGSNIKPGD-TNLPAIKRLSDNFKSTGKFFTVQLTPAREPSDWSPLYSRDEISHYVTVIRSADINSIYKLIYKRIYPLINCPEGFVKVRGLCQLQTATIKYCKTEDKQLRAVGSTWQEECSEFKCTNSGIEVVSRKCLGYDGQCYSTEEYFPCTQVTGSLSWKCRCKLRTRTNKKGKKVES------LHYQSIATSKEKVLTESGRQCVFPFKYQNKWLYNCDEYNG-RYWCSYHRMHETGSWGYCKIKESKNRCTDPFNPSKVYIEGERWNHGCNVMECTKTGLKRIPKCQAADKTCKALNEDGYSCHYSGSLHKNCICVQNSAGWGKNEVVTGDKAKEVSSSGGSVVSVC---VDRSTGKRYEQGQEWVNSCFKYKCTAKG-GELVESGCKQKDGKCRKIGEKG-FDCVVKGKSAKSCECAKDS 1619          
BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Match: A0A267F293 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000911g1 PE=4 SV=1)

HSP 1 Score: 508.834 bits (1309), Expect = 4.643e-146
Identity = 347/1166 (29.76%), Postives = 568/1166 (48.71%), Query Frame = 3
Query: 1701 QYCISSCPVDYDTDAQFCK-----------RRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSV----VCGVNKELQDCASSNGGECNTK--CACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITI------NNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVF-NNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYK---LKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYN--SSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDK-LYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCT-LNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCA-RQVTSQCI--FD-GKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIK-DKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI 5075
            + C+ S  VDY    + CK           RR A C SL      C+++       K R C  +K   NSV      G  +   D          ++  CACK G+V       C+P E  C     LD+ F++DG++ V+   F  +K  +++ +T+   S  +VR  IAV+G  +PT+ L ++     D M  V ++K  PG     L  A  +  K++++   GAR    + I ++ + +++    +  + +E++ LK     +  +T+        D  I   +  +  R    L+  + K+     K   C  GY++ G  CID+NEC +    CH++S C+N PGSF+C C     +       + K E+C  + K                          C  G+ +D  +G C+DINEC  NPCL D ATC N+ GSF C C  GF+Y+ +FGCVDIDEC+    C+G+G C N +GS++C C  GY Y    GC DIDEC A    C+G +  C NT GS+ C C  G  Y+   C+DIDEC +++  C   +TC+N   G+ C C  G  + +   + C  D+   + Q K D +F ID S+ +K+    +KD L  ++  +K   LK L++  S   +   G +++K      +  ++    D I  +      ++ L   K     H +    S+ +V  IT +     D + +  IK      K  G+   + +    + ++W  ++  +EI  +V  +    + TI+K+IY++   LI CPEGF+ +  +CQ +T    YCK +DK L A+ STW   C+ + C  +G+ V+   C+G+DG CY  +E+FPCT + G+L+W+C+CK+   T+++ KK +       +++   AT +   L +   +CV PFK++NKW Y C +Y     WC++H  ++ G +  C  ++  ++C   F+  K+Y EG++W+ GCNV+ C         +CQ  + TC  LN D Y C   G  + +C+C+Q++ G+G+N  +  DK     +       +     D S+G+RYE G+ W   CF  +C   G  ++++  C   DGKC + G    F C + GK  ++C CA D+     +S         VV  +K++ +
Sbjct:  953 RACLKSGKVDYTKIYKSCKFDVCQYRKAKNRRKAVCESLETLYNACLAKGFRVSWRKRRFC-PLKCGANSVFRRKTSGCPRTCADLGRRRKCRLPSREGCACKEGYVL--NGKKCVP-ERICGKTVPLDLAFVVDGTDDVSSDEFETLKNLIIKLLTKYRLSSNSVRPAIAVFGRTSPTVSLFNQRPGKADFMAFVRKMKK-PGGSADNLDAALRVANKVMMNPMGGARPAVPKYILLLKK-SKVPASSESVVKSELDSLKKANVHVVAVTLPPTKPGKTDTAIG-GLSVIPARVPPYLA--IPKITKELEKTAGCTSGYKRQGKRCIDVNECAAKVPPCHSDSTCVNLPGSFICLCREGFRHDRSRGCVSLKKEKCPSKPK--------------------------CDNGYRFDKAKGKCVDINECKENPCLGDSATCVNTPGSFKCKCKTGFEYNKYFGCVDIDECKGKDPCAGSGKCINTLGSFKCTCDEGYTYKTGLGCKDIDECAAGSHNCKGGSK-CINTAGSYNCSCDSGYRYESNKCIDIDECAEDSKLCDASATCVNKKPGYECQCKLGSNWVHKAGRGCVPDEKKGMSQRKVDFIFTIDGSDKLKSSEKDIKDFLTRLLNKFKANELKGLSVRASAIIMGKRGGADIKF-----SQADLSGIDDKIKALNFPGGKANLLDGMKKANREHAKARNGSTPIVFLITGSNIKPGD-TNLPAIKRLSDNFKSTGKFFTVQLTPAREPSDWSPLYSRDEISHYVTVIRSADINTIYKLIYKRIYPLINCPEGFVKVRGLCQLQTATIKYCKTEDKQLRAVGSTWQEECSEFKCTNSGIEVVSRKCLGYDGQCYSTEEYFPCTQVTGSLSWKCRCKLRTRTNKKGKKVES------LHYQSIATSKEKVLTESGRQCVFPFKYQNKWLYNCDEYNG-RYWCSYHRMHETGSWGYCKIKESKNRCTDPFNPSKVYIEGERWNHGCNVMECTKTGLKRIPKCQAADKTCKALNEDGYSCHYSGSLHKNCICVQNSAGWGKNEVVTGDKAKEVSSSGGSVVSVC---VDRSTGKRYEQGQEWVNSCFKYKCTAKG-GELVESGCKQKDGKCRKIGEKG-FDCVVKGKSAKSCECAKDSAGNPTLSG--------VVSGRKMIQV 2056          
BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Match: A0A1I8G2Y1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 509.99 bits (1312), Expect = 3.614e-145
Identity = 347/1166 (29.76%), Postives = 568/1166 (48.71%), Query Frame = 3
Query: 1701 QYCISSCPVDYDTDAQFCK-----------RRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSV----VCGVNKELQDCASSNGGECNTK--CACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITI------NNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVF-NNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYK---LKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYN--SSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDK-LYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCT-LNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCA-RQVTSQCI--FD-GKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIK-DKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI 5075
            + C+ S  VDY    + CK           RR A C SL      C+++       K R C  +K   NSV      G  +   D          ++  CACK G+V       C+P E  C     LD+ F++DG++ V+   F  +K  +++ +T+   S  +VR  IAV+G  +PT+ L ++     D M  V ++K  PG     L  A  +  K++++   GAR    + I ++ + +++    +  + +E++ LK     +  +T+        D  I   +  +  R    L+  + K+     K   C  GY++ G  CID+NEC +    CH++S C+N PGSF+C C     +       + K E+C  + K                          C  G+ +D  +G C+DINEC  NPCL D ATC N+ GSF C C  GF+Y+ +FGCVDIDEC+    C+G+G C N +GS++C C  GY Y    GC DIDEC A    C+G +  C NT GS+ C C  G  Y+   C+DIDEC +++  C   +TC+N   G+ C C  G  + +   + C  D+   + Q K D +F ID S+ +K+    +KD L  ++  +K   LK L++  S   +   G +++K      +  ++    D I  +      ++ L   K     H +    S+ +V  IT +     D + +  IK      K  G+   + +    + ++W  ++  +EI  +V  +    + TI+K+IY++   LI CPEGF+ +  +CQ +T    YCK +DK L A+ STW   C+ + C  +G+ V+   C+G+DG CY  +E+FPCT + G+L+W+C+CK+   T+++ KK +       +++   AT +   L +   +CV PFK++NKW Y C +Y     WC++H  ++ G +  C  ++  ++C   F+  K+Y EG++W+ GCNV+ C         +CQ  + TC  LN D Y C   G  + +C+C+Q++ G+G+N  +  DK     +       +     D S+G+RYE G+ W   CF  +C   G  ++++  C   DGKC + G    F C + GK  ++C CA D+     +S         VV  +K++ +
Sbjct: 1844 RACLKSGKVDYTKIYKSCKFDVCQYRKAKNRRKAVCESLETLYNACLAKGFRVSWRKRRFC-PLKCGANSVFRRKTSGCPRTCADLGRRRKCRLPSREGCACKEGYVL--NGKKCVP-ERICGKTVPLDLAFVVDGTDDVSSDEFETLKNLIIKLLTKYRLSSNSVRPAIAVFGRTSPTVSLFNQRPGKADFMAFVRKMKK-PGGSADNLDAALRVANKVMMNPMGGARPAVPKYILLLKK-SKVPASSESVVKSELDSLKKANVHVVAVTLPPTKPGKTDTAIG-GLSVIPARVPPYLA--IPKITKELEKTAGCTSGYKRQGKRCIDVNECAAKVPPCHSDSTCVNLPGSFICLCREGFRHDRSRGCVSLKKEKCPSKPK--------------------------CDNGYRFDKAKGKCVDINECKENPCLGDSATCVNTPGSFKCKCKTGFEYNKYFGCVDIDECKGKDPCAGSGKCINTLGSFKCTCDEGYTYKTGLGCKDIDECAAGSHNCKGGSK-CINTAGSYNCSCDSGYRYESNKCIDIDECAEDSKLCDASATCVNKKPGYECQCKLGSNWVHKAGRGCVPDEKKGMSQRKVDFIFTIDGSDKLKSSEKDIKDFLTRLLNKFKANELKGLSVRASAIIMGKRGGADIKF-----SQADLSGIDDKIKALNFPGGKANLLDGMKKANREHAKARNGSTPIVFLITGSNIKPGD-TNLPAIKRLSDNFKSTGKFFTVQLTPAREPSDWSPLYSRDEISHYVTVIRSADINTIYKLIYKRIYPLINCPEGFVKVRGLCQLQTATIKYCKTEDKQLRAVGSTWQEECSEFKCTNSGIEVVSRKCLGYDGQCYSTEEYFPCTQVTGSLSWKCRCKLRTRTNKKGKKVES------LHYQSIATSKEKVLTESGRQCVFPFKYQNKWLYNCDEYNG-RYWCSYHRMHETGSWGYCKIKESKNRCTDPFNPSKVYIEGERWNHGCNVMECTKTGLKRIPKCQAADKTCKALNEDGYSCHYSGSLHKNCICVQNSAGWGKNEVVTGDKAKEVSSSGGSVVSVC---VDRSTGKRYEQGQEWVNSCFKYKCTAKG-GELVESGCKQKDGKCRKIGEKG-FDCVVKGKSAKSCECAKDSAGNPTLSG--------VVSGRKMIQV 2947          

HSP 2 Score: 72.4034 bits (176), Expect = 1.282e-8
Identity = 94/373 (25.20%), Postives = 138/373 (37.00%), Query Frame = 3
Query:  849 NRTICPIGYDKEGTYCIQ--KTCERGLLL-----NGFCL--DTICKT-NYEFDGKVCKKCLTSTHSYCSAMDWT---CPMGLIEIGSGPHMQCVMICPKRYAQFGKSCYDISVTGCPEGYLH--FEDNTCIEESKCPDGFEIFGTECVRK--LTYVGFIINSKEVQL-CPTDYIEYQGKCFKLTD--------------NACPDGTLAIEDSHYCVSTCPQGFNNVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQVLDLL---SFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCPVDYDTDAQFCKRRPAECP-SLSLSNGTCVSECPEGHVPKNRICVSV 1859
             R  CP GY + G  C+   + C R   +      G C+  + ICK+  + F+ +  +KC   T             C  GL+         C   CP    +    C  I    C +G++   F    CI  + C + F I G  CVRK  + Y+G     + V + CP  Y+   G+   L +              +AC  GT     +  C+  CP G       C   S+C+   F++  +C  +CP G   S   RSC V   C   ++L +    +  CV   AC + Y V      C +  +C    Y L   C  SCP  +      C+     C   L      CV  CP G   +   C  V
Sbjct:  884 KRKFCPSGYRRRGRTCVGYYRVCPRTFTMIRNKRGGRCIKFNKICKSGKFLFNNRCWRKCPRGTRRSSRRSCKRKRGCKRGLVYYSG----SCRSKCPSGTRRRRWYCLKI----CKKGFMRVGFRRQRCIR-AYCYNKFRI-GRSCVRKCPVGYIGIRRKCRRVSISCPKAYVRL-GRALCLRNCPAGFTKRGKQCAYSACRKGTYRYRGT--CMKGCPAGTKRRGSYCFKRSKCRFGRFAYGKRCLVRCPPGHYRSFARRSC-VTRLCPSGRLLLIKPSGARSCVR--ACPRTYTVVRSRGSC-KPTRCPKGMYRLGITCRKSCPKGFRARRGRCRAVRKSCKDGLFRLRRRCVKRCPRGFKARGGRCRRV 1239          

HSP 3 Score: 71.2478 bits (173), Expect = 3.022e-8
Identity = 67/271 (24.72%), Postives = 95/271 (35.06%), Query Frame = 3
Query:  804 CPPYFEKINSSTCESNRTICPIGYDKEGTYCIQKTCERGLL-LNGFCLDTICKTNYEFDGKVC---KKCLTSTHSYCSAMDWTCPMGLIEIGSGPHMQCV-MICPK-RYAQFGKSCYDISVTGCPEGYLHFEDNTCIEESKCPDGFEIFGTECVRKLTYVGFIINSKEVQLCPTDYIEYQGKCFKLTDNACPDGTLAIEDSHYCVSTCPQGFNNVQKACI-VSSECKSNEFSFMGKCHEKCP-------FGTKDSNNTRSCKVVPNCEKNQ 1574
            CP  + ++  + C  N   CP G+ K G  C    C +G     G C+   C    +  G  C    KC     +Y       CP G     S     CV  +CP  R      S     V  CP  Y         + ++CP G    G  C +                CP  +   +G+C +    +C DG   +     CV  CP+GF      C  V   CK   F F  +C +KCP       +        R C+ + +C+K Q
Sbjct: 1042 CPKAYVRLGRALCLRN---CPAGFTKRGKQCAYSACRKGTYRYRGTCMKG-CPAGTKRRGSYCFKRSKCRFGRFAYGKRCLVRCPPGHYR--SFARRSCVTRLCPSGRLLLIKPSGARSCVRACPRTYTVVRSRGSCKPTRCPKGMYRLGITCRKS---------------CPKGFRARRGRC-RAVRKSCKDGLFRLR--RRCVKRCPRGFKARGGRCRRVPFRCKKGTFRFRRRCVKKCPKKYTGRVYKRARRAPVRYCRPIRSCKKGQ 1288          
BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Match: A0A1I8I5M9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 504.597 bits (1298), Expect = 1.303e-143
Identity = 351/1162 (30.21%), Postives = 558/1162 (48.02%), Query Frame = 3
Query: 1701 QYCISSCPVDYDTDAQFCK-----------RRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSV----VCGVNKELQDCASSNGGECNTK--CACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINNDVDIPRSI--YQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVF-NNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYK---LKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYN--SSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDK-LYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCT-LNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCA-RQVTSQCI--FD-GKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIK-DKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI 5075
            + C+ S  VDY    + CK           RR A C SL      C+++       K R C  +K   NSV      G  +   D          ++  CACK G+V   G+  C+P E  C     LD+ F++DG++ V+   F  +K  +++ +T+   S  +VR  IAV+G  +PT+ L ++     D M  V ++K  PG     L  A  +  K++++   GAR    + I                LL + + +     R T   I     IP  +  Y    + TKEL            K   C  GY++ G  CID+NEC +    CH++S C+N PGSF+C C     +       + K E+C  + K                          C  G+ +D   G C+DINEC  NPCL D ATC N+ GSF C C  GF+Y+ +FGCVDIDEC+    C+G+G C N +GS++C C  GY Y    GC DIDEC A    C+G +  C NT GS+ C C  G  Y+   C+DIDEC +++  C   +TC+N   G+ C C  G  + +   + C  D+   + Q K D +F ID S+ +K+    +KD L  ++  +K   LK L++  S   +   G +++K      +  ++    D I  +      ++ L   K     H +    S+ +V  IT +     D + +  IK      K  G+   + +    + ++W  ++  +EI  +V  +    + TI+K+IY++   LI CPEGF+ +  +CQ +T    YCK +DK L A+ STW   C+ + C  +G+ V+   C+G+DG CY  +E+FPCT + G+L+W+C+CK+   T+++ KK +       +++   AT +   L +   +CV PFK++NKW Y C +Y     WC++H  ++ G +  C  ++  ++C   F+  K+Y EG++W+ GCNV+ C         +CQ  + TC  LN D Y C   G  + +C+C+Q++ G+G+N  +  DK     +       +     D S+G+RYE G+ W   CF  +C   G  ++++  C   DGKC + G    F C + GK  ++C CA D+     +S         VV  +K++ +
Sbjct: 1541 RACLKSGKVDYTKIYKSCKFDVCQYRKAKNRRKAVCESLETLYNACLAKGFRVSWRKRRFC-PLKCGANSVFRRKTSGCPRTCADLGRRRKCRLPSREGCACKEGYV-LNGKK-CVP-ERICGKVVPLDLAFVVDGTDDVSSDEFETLKNLIIKLLTKYRLSSNSVRPAIAVFGRTSPTVSLFNQRPGKADFMAFVRKMKK-PGGSADNLDAALRVANKVMMNPMGGARPAVPKYI----------------LLLKKSKVPASSERKTDTAIGGLSVIPARVPPYLAIPKITKELE-----------KTAGCTSGYKRQGKRCIDVNECAAKVPPCHSDSTCVNLPGSFICLCREGFRHDRSRGCVSLKKEKCPSKPK--------------------------CDNGYRFDKANGKCVDINECKENPCLGDSATCVNTPGSFKCKCKTGFEYNKYFGCVDIDECKGKDPCAGSGKCINTLGSFKCTCDEGYTYKTGLGCKDIDECAAGSHNCKGGSK-CINTAGSYNCSCDSGYRYESNKCIDIDECAEDSKLCDASATCVNKKPGYECQCKLGSNWVHKAGRGCVPDEKKGMSQRKVDFIFTIDGSDKLKSSEKDIKDFLTRLLNKFKANELKGLSVRASAIIMGKKGGADIKF-----SQADLSGIDDKIKALNFPGGKANLLDGMKKANREHAKARNGSTPIVFLITGSNIKPGD-TNLPAIKRLSDNFKSTGKFFTVQLTPAREPSDWSPLYSRDEISHYVTVIRSADINTIYKLIYKRIYPLINCPEGFVKVRGLCQLQTATIKYCKTEDKQLRAVGSTWQEECSEFKCTNSGIEVVSRKCLGYDGQCYSTEEYFPCTQVTGSLSWKCRCKLRTRTNKKGKKVES------LHYQSIATSKEKVLTESGRQCVFPFKYQNKWLYNCDEYNG-RYWCSYHRMHETGSWGYCKIKESKNRCTDPFNPSKVYIEGERWNHGCNVMECTKTGLKRIPKCQAADKTCKALNEDGYSCHYSGSLHKNCICVQNSAGWGKNEVVTGDKAKEVSSSGGSVVSVC---VDRSTGKRYEQGQEWVNSCFKYKCTAKG-GELVESGCKQKDGKCRKIGEKG-FDCVVKGKSAKSCECAKDSAGNPTLSG--------VVSGRKMIQV 2617          

HSP 2 Score: 68.9366 bits (167), Expect = 1.320e-7
Identity = 90/352 (25.57%), Postives = 133/352 (37.78%), Query Frame = 3
Query:  849 NRTICPIGYDKEGTYCIQ--KTCERGLLL-----NGFCL--DTICKT-NYEFDGKVCKKCLTSTHSYCSAMDWT---CPMGLIEIGSGPHMQCVMICPKRYAQFGKSCYDISVTGCPEGYLH--FEDNTCIEESKCPDGFEIFGTECVRK--LTYVGFIINSKEVQL-CPTDYIEYQGKCFKLTD--------------NACPDGTLAIEDSHYCVSTCPQGFNNVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQVLDLL---SFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCPVDYDTD-------AQFCKRRPAECP 1778
             R  CP GY + G  C+   + C R   +      G C+  + ICK+  + F+ +  +KC   T             C  GL+         C   CP    +    C  I    C +G++   F    CI  + C + F I G  CVRK  + Y+G     + V + CP  Y+   G+   L +              +AC  GT     +  C+  CP G       C   S+C+   F++  +C  +CP G   S   RSC V   C   ++L +    +  CV   AC + Y V      C +  +C    Y L   C  SCP  +           + CKRRP   P
Sbjct:  729 KRKFCPSGYRRRGRTCVGYYRVCPRTFTMIRNKRGGRCIKFNKICKSGKFLFNNRCWRKCPRGTRRSSRRSCKRKRGCKRGLVYYSG----SCRSKCPSGTRRRRWYCLKI----CKKGFMRVGFRRQRCIR-AYCYNKFRI-GRSCVRKCPVGYIGIRRKCRRVSISCPKAYVRL-GRALCLRNCPAGFTKRGKQCAYSACRKGTYRYRGT--CMKGCPAGTKRRGSYCFKRSKCRFGRFAYGKRCLVRCPPGHYRSFARRSC-VTRLCPSGRLLLIKPSGARSCVR--ACPRTYTVVRSRGSC-KPTRCPKGMYRLGITCRKSCPKGFRARRGRCRAVRKSCKRRPVPTP 1063          
BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Match: A0A267GFI7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016567g2 PE=4 SV=1)

HSP 1 Score: 486.878 bits (1252), Expect = 1.798e-143
Identity = 333/1058 (31.47%), Postives = 543/1058 (51.32%), Query Frame = 3
Query: 1947 CACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFI----TINNDVDIPRS-IYQLSVRTTKELS-QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA----PKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKG--FVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKSYFALT-TGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYNSSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWD-DKLYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCT-LNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCARQVT-SQCI--FDG-KIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIKDKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALD--NDELVLVSNEGGKI 5036
            C CKPGFV       C+P++S C +   +D+ F+I+G++ ++   F  +K  +++ +T+   S  +VR  + V+G  +PT+ L  + +   + M  V ++K   G     L  A  +  K+++    G R    + + V+ + +   T DK  +  ++  LK+   R+  +    T  + VD   S +  +S R     S   ++K  + T     C  G++   D+C DINEC +   ACH+++ C+N PGSF+C CS+   + +   GC     PK  +C  E K                          C +G+TYD  E  C+D+NEC  NPCL D+ATC N  GSF C CP GF+Y+ +FGCVD+DEC+  K  C G G C NM+GSY C C++G+ Y    GC D+DEC++ +   C+G +  C N  GS+ C C  G  Y  + CVDIDEC +N+  C   + C+N   G+ C C  G  +V+    + C  DK   +   K D  F ID S+ +K  +  ++  +  ++  +  K +A + +  ++   G   E  V + +     + +  +SI  +GG+S L  G+K+      +      S +V+F+          S + EIK    + K +G+   + +     S++W V++   EI  +V  +    +T I+K+ Y +   LI CP GF+  D +CQ +T+   YCK D  +L  + +TW   C+ + C ++GV VL   C+G+DG CY  +E+FPCT + G+LNW+C+C++   TD+ + K  Q + Y  I     A    T + D+   CV PFK+ NKW+Y+C  Y+    WC+ +  ++ G +  C  + + ++C   FD  K+Y EGD+W+ GCNV+ C         +C+  + TC  L+AD Y C++G +Q+ +C+C+Q++ G+G+N  +        T   G    M  C D SSG++YE G++W   CFT +C   G  +++  KC   DGKC   G    F C I  K   NC C+ D   + +++ + EG +I
Sbjct:  209 CVCKPGFVL--NGRACVPEKS-CGAVVPIDVAFVIEGTDDISTDEFEALKSLVVKLLTKYRLSLKSVRPSMVVFGRVSPTVSLFSEHQGKAEFMNYVRKLKRTGG-STDNLEAALRVVNKVLMQAIGGFRPEVPKYVIVMKKSSVPATSDK-PVRNQLEKLKESKVRVIAVNLPKTAKSSVDPTMSGLTVISARMPPYTSIPAITKELEST---AGCPMGFKTQNDSCTDINECKATVPACHSDATCVNLPGSFMCLCSDGLLH-DRARGCVKPDKPK-ADCPLEPK--------------------------CKQGYTYDKNEKKCVDVNECKENPCLSDRATCTNLAGSFRCDCPSGFKYNKYFGCVDVDECKLKKDPCMGAGKCINMLGSYNCTCNDGFTYQTGVGCQDVDECKSNATNNCKGGSK-CVNIAGSYNCTCGSGYRYSSESCVDIDECAENSKICDASAICVNQKPGYKCQCKLGDNWVYKTG-EGCIPDKNKGMADRKVDFFFLIDVSDKLKGSIRDIQGFINGLLQQFNTKEVAGLSARVSVVLMGKKGEAVVAFSQSPLVGIADKINSIAPLGGESNLLQGMKTVAGE--KSKARRGSTVVVFLITASSIPVGDSSLTEIKRYSDDFKSNGKFFTVQLSSSASSSQWSVLYSSNEISLYVTVIRSSEVTKIYKLFYLRVYPLINCPAGFVKNDGLCQLQTSTVKYCKTDAGELRPVGTTWQEECSQFKCTDSGVEVLYRKCLGYDGFCYSTEEYFPCTQVTGSLNWKCRCRLKTVTDKASGKKIQSLSYQSI-----AAANETMMTDKGIACVFPFKYRNKWHYSCDQYEG-KYWCSFNRNHKTGSWGYCKIKASKNRCTDPFDASKMYVEGDRWNYGCNVMECTHTGLKRIPKCEAADKTCKNLHADGYSCRIGDKQHSNCLCVQNSSGWGKNEIVSGDKATVITGSGGAVVSM--CVDISSGKKYEQGQQWTTSCFTYKCTATG-GQLLQAKCQQKDGKCRNIGEKG-FDCMISDKARANCECSKDASGNPMLVATFEGRQI 1216          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Match: fbn2a (fibrillin 2a [Source:ZFIN;Acc:ZDB-GENE-090112-4])

HSP 1 Score: 140.198 bits (352), Expect = 1.478e-32
Identity = 100/265 (37.74%), Postives = 144/265 (54.34%), Query Frame = 3
Query: 2610 CIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C DI+EC S  + C  N+ CIN+PGS+ C CS   + L+P   C  + E    E   +         S   C    G   C C  GF     ++ C+DI+EC   PC +  TC+N+ GS++C C  GF+ +++  C D+DEC    S++C  NG C N +GS++C C  GYE   D K CID++EC      C      CQN  GSF+C+CP G   ++  CVD++EC+++ N C  GS C N+ G F C C  GFV +++ + C
Sbjct: 1702 CEDIDECSSSEILCQRNADCINSPGSYRCECSEGFK-LSPIGACIDRNE--CLENPNLC--------SHGKCVDMPGGYICTCNTGFKATPNRKMCMDIDECVRQPCKN-GTCKNTVGSYNCLCNAGFELTLNNQCTDVDECSGPYSQLCR-NGQCMNSVGSFQCLCDEGYELTLDGKNCIDVNECLILPGTCAPG--TCQNLDGSFRCLCPKGYTVQDDHCVDVNECEEDPNICLFGS-CFNNPGSFECVCKPGFVLSDNGRRC 1950          

HSP 2 Score: 118.242 bits (295), Expect = 6.182e-26
Identity = 101/280 (36.07%), Postives = 134/280 (47.86%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSV---HFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GYQ   D   C DI+EC       +  C N+ GS+ C C      L P+      ++EC  E  I   CD         C++  G   C+C  GFT  +  + CID+NEC +N   C+    C N+ GSF C C  G  YSV     GC DIDEC+ D+  C  N  C N+ GS+ C C  G+     K C D+DEC      C  D   C NTPGSF+C C  G +     C DIDEC  +    E   C+N  G + C C  GF+ + D++ C+
Sbjct: 1079 SCSTGYQSTPDRKGCTDIDECTIENGGCDLLCTNSEGSYTCSCGQ-RYALMPDLRSCTDVDECEEEPDI---CDG------GQCTNVPGEYHCLCYDGFTSSMTTKSCIDVNECELNSNICM-FGECENTKGSFICHCEVG--YSVKKGSSGCTDIDECQIDAHNCDRNANCVNIPGSFSCTCMEGWIGNGVK-CDDVDECIRGTHQCSPDGE-CVNTPGSFRCACTEGYKGDGFTCSDIDECADSTHMCENGQCLNIPGSYQCECEIGFISDPDSRTCQ 1343          

HSP 3 Score: 113.235 bits (282), Expect = 1.887e-24
Identity = 91/266 (34.21%), Postives = 127/266 (47.74%), Query Frame = 3
Query: 2616 DINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCP--VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC    S+  H   R  NT GSF C C+        E  C   I+EC     +              C ++ G   C C  G+   + + + C+DI+EC  NP L +  +C+N+ GS+ C CP G Q S     C D++EC  S     NG C N+ G+Y C+C  GY+   D KGC DIDEC      C   +++C N+ GS+ C C     L    + C D+DEC++     +G  C N  G ++C C  GF  +  TK C
Sbjct:  926 DINECKVFPSICAHGTCR--NTIGSFRCRCNGGFALDAEERNCT-DIDECKISPDLCGH---------GACVNTPGSFECDCLPGYESGFMMMKNCMDIDECERNPGLCRGGSCKNTEGSYQCVCPPGHQLSTDGDACEDVNECELSATLCRNGECVNVAGTYHCSCSTGYQSTPDRKGCTDIDECTIENGGC---DLLCTNSEGSYTCSCGQRYALMPDLRSCTDVDECEEEPDICDGGQCTNVPGEYHCLCYDGFTSSMTTKSC 1176          

HSP 4 Score: 110.538 bits (275), Expect = 1.319e-23
Identity = 117/359 (32.59%), Postives = 152/359 (42.34%), Query Frame = 3
Query: 2427 LKDEGHRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEEC----------SFERKIISICDAVTCRSPANCSSSN-----GVEFCVCPRGFTYDLKEGC--------------IDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS---VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQN-NTC-PEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            L DEG+ LT +   N +D+   +  L         Q+L   F   C      KGY    D+C+D+NEC  D   C   S C N PGSF C C        P F  +     C           FE    SI  A        C S+      G    +CP+      ++ C              +D+NEC  NP +     C N+ GSF C CP G+  +   VH  CVD+DEC     C GNG C N+IG + C C  G+E      C DI+EC  +  LC      C NT G ++C CP G   +E G  C D+DEC +  + C   G TC N  G F C C  G V   D + C+
Sbjct: 1854 LCDEGYELT-LDGKNCIDVNECLI-LPGTCAPGTCQNLDGSFRCLCP-----KGYTVQDDHCVDVNECEEDPNICLFGS-CFNNPGSFECVCK-------PGFVLSDNGRRCYDTRVSFCFTRFENGKCSIPQAFNTTKARCCCSAMPKEGWGYPCELCPKEHEAGFRDLCPFGVGIIPGIDDTSVDVNECVENPGICHNGHCINTDGSFRCECPMGYTLNYTGVH--CVDVDECAVGNPC-GNGTCTNVIGGFECNCEEGFEPGPMMACEDINECGQNPLLCA---FRCINTFGGYECSCPSGYTLREDGRMCQDLDECVEGLHDCDTRGMTCKNLIGTFMCICPPGMVRRMDDELCQ 2191          

HSP 5 Score: 106.301 bits (264), Expect = 2.825e-22
Identity = 97/286 (33.92%), Postives = 131/286 (45.80%), Query Frame = 3
Query: 2610 CIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD-------AVTCRSPANCSSSNG----VEFC----------VCPRGFTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C D NEC +      N RC+NT GS++C C    +       C    +   F   + ++C        A+T +S   C   +G     E C          +CP G  Y       DI+EC + P + K   C N+ GS+ C C  G+  S+    CVD+DEC  S K C  N IC+N  GSY C+C  GY   ED + C D+DECQ  +  CQ    +C NT G F C CP G       C+D +EC  + + C     C NS G F+C C +GF  +     CE
Sbjct: 2190 CQDENECRTKPGICENGRCVNTIGSYMCECGEGFQPDPTHTQCLDYRQGFCFTEVLQTMCQMSSSSRTAIT-KSQCCCDGGHGWGNQCELCPLPGTAKYKKLCPHGHGYATDG--TDIDECKVIPDVCKNGRCINTMGSYRCHCNSGYTASIAGTACVDLDECAMSPKPC--NFICKNTEGSYACSCPRGYILQEDSRSCKDLDECQVKRHNCQ---FMCVNTIGGFTCKCPPGFTQHHTACIDHNECAAEPSLCGAKGLCQNSPGSFSCECQRGFSLDTSGLHCE 2467          

HSP 6 Score: 103.99 bits (258), Expect = 1.264e-21
Identity = 89/263 (33.84%), Postives = 123/263 (46.77%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-PKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCT 3341
            G GY   G    DI+EC  +     N RCINT GS+ C+C  N  Y     G A   ++EC+   K            P N  C ++ G   C CPRG+   +    C D++EC +     +  C N+ G F C CP GF    H  C+D +EC  +  +C   G+CQN  GS+ C C  G+        C D++EC ++ + CQ     C+N  G ++C CP G    Y+   CVD +EC    TC   S C N+ G F C 
Sbjct: 2294 GHGYATDG---TDIDECKVIPDVCKNGRCINTMGSYRCHC--NSGYTASIAGTACVDLDECAMSPK------------PCNFICKNTEGSYACSCPRGYILQEDSRSCKDLDECQVKRHNCQFMCVNTIGGFTCKCPPGFTQH-HTACIDHNECAAEPSLCGAKGLCQNSPGSFSCECQRGFSLDTSGLHCEDVNECDSNHR-CQ---FGCENMLGGYRCNCPQGYTQHYQWNQCVDENECSTPGTCGSAS-CYNTLGSFKCA 2533          

HSP 7 Score: 101.679 bits (252), Expect = 6.747e-21
Identity = 74/185 (40.00%), Postives = 99/185 (53.51%), Query Frame = 3
Query: 2859 GFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY--KEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            GF  D++ G  +DI+ECN  P +     C N  GSF C CP+GF Y+ V   C DIDEC  S+I C  N  C N  GSYRC C  G++      CID +EC  +  LC   +  C + PG + C C  G +     K C+DIDEC +   C  G TC N+ G +NC CN GF    + +  ++D+
Sbjct: 1650 GFEIDIETGKPVDIDECNEIPGICASGVCINQIGSFRCECPRGFSYNDVLLICEDIDECSSSEILCQRNADCINSPGSYRCECSEGFKLSPIGACIDRNECLENPNLCS--HGKCVDMPGGYICTCNTGFKATPNRKMCMDIDECVRQ-PCKNG-TCKNTVGSYNCLCNAGFELTLNNQCTDVDE 1830          

HSP 8 Score: 96.6709 bits (239), Expect = 2.412e-19
Identity = 66/187 (35.29%), Postives = 95/187 (50.80%), Query Frame = 3
Query: 2811 ANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
              C ++ G   C C  G+T  +    C+D++EC M+P      C+N+ GS+ C CP+G+        C D+DEC+  +  +   +C N IG + C C  G+       CID +EC A   LC G   +CQN+PGSF C C  G      G  C D++EC  N+ C  G  C N  GG+ C C +G+ 
Sbjct: 2317 GRCINTMGSYRCHCNSGYTASIAGTACVDLDECAMSPKPCNFICKNTEGSYACSCPRGYILQEDSRSCKDLDECQVKRH-NCQFMCVNTIGGFTCKCPPGFTQ-HHTACIDHNECAAEPSLC-GAKGLCQNSPGSFSCECQRGFSLDTSGLHCEDVNECDSNHRCQFG--CENMLGGYRCNCPQGYT 2498          

HSP 9 Score: 96.2857 bits (238), Expect = 3.131e-19
Identity = 89/287 (31.01%), Postives = 126/287 (43.90%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCV-------DIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            D  C +G+ +  G  C D+NEC+       N RC+NT GSF C CS      +    C   ++E C          D   C  P   S    ++ C C  G  +          EC   P             ++  CP+G  ++     +       DI+EC+       +G C+N IGS+RC C+ G+    E++ C DIDEC+ S  LC   +  C NTPGSF+C C  G E      K C+DIDEC++N     G +C N+ G + C C  G   + D   CE
Sbjct:  791 DPVCQRGFARPRGTICEDVNECEVFPGVCPNGRCVNTRGSFKCQCSEGLTLDSTGRICVDIRLEHCYLTY------DEDECVQPV--SGRFRMDACCCTVGAAWG--------KECEACP-------EPGTREYEALCPRGPGFANRGDVLTGRPVYKDINECKVFPSICAHGTCRNTIGSFRCRCNGGFALDAEERNCTDIDECKISPDLC--GHGACVNTPGSFECDCLPGYESGFMMMKNCMDIDECERNPGLCRGGSCKNTEGSYQCVCPPGHQLSTDGDACE 1052          

HSP 10 Score: 91.6633 bits (226), Expect = 6.422e-18
Identity = 101/318 (31.76%), Postives = 138/318 (43.40%), Query Frame = 3
Query: 2616 DINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLD--KATCRNSYGSFDC----------------------------FCPK---------------GFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQP 3416
            DI+EC  L   C   + C+NT GSF C C     YLN +      I+EC     I           P  C ++ G   CVCP+ +        + +N    N C+D  K+ C  +Y    C                             CP+               GF+  +  G  VDIDEC +      +G+C N IGS+RC C  G+ Y +    C DIDEC +S+ LCQ  N  C N+PGS++C C  G +    G C+D +EC +N N C  G  C++  GG+ CTCN GF    + K C +D    V QP
Sbjct: 1503 DIDECQELPGLCQGGN-CVNTFGSFQCDCPGGY-YLNADSRICEDIDECVVNPGICG---------PGTCYNTLGNYTCVCPQEY--------MQVN--GGNNCMDMRKSLCFRNYNGTACENELAFNITHRICCCAYNMGKAWNKPCQACPRPGTEGFRTLCGNIVPGFEIDIETGKPVDIDECNEIPGICASGVCINQIGSFRCECPRGFSYNDVLLICEDIDECSSSEILCQ-RNADCINSPGSYRCECSEGFKLSPIGACIDRNECLENPNLCSHGK-CVDMPGGYICTCNTGFKATPNRKMC-MDIDECVRQP 1796          

HSP 11 Score: 85.8853 bits (211), Expect = 3.583e-16
Identity = 93/320 (29.06%), Postives = 129/320 (40.31%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCID---------------------------IDECQASKKLCQGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQP 3416
            C +G+   G  C D++EC   +  C  +  C+NTPGSF C C+  E Y    F C+  I+EC+         D+        C +  G   C C  GF  D     C DI+EC          C N  G+F C C +G++ +     C D+DEC D   C  NG C N  GSY C C   +E      GC+D                           +        L +     C+  P      +  +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N D++ CE D    VV P
Sbjct: 1248 CMEGWIGNGVKCDDVDECIRGTHQCSPDGECVNTPGSFRCACT--EGYKGDGFTCS-DIDECA---------DSTHMCENGQCLNIPGSYQCECEIGFISDPDSRTCQDIDECTFQVICVFGLCENLPGAFRCVCDEGYELNRSGINCTDVDECLDPINCV-NGHCVNTPGSYECNCPTDFELNPTGIGCVDPRVGFCFLEAVPRGRGGLACSVEVGVGVSRSSCCCSLGRAWGNPCEGCPPVNTSDYNTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCVNTFGSFQCDCPGGYYLNADSRICE-DIDECVVNP 1553          

HSP 12 Score: 82.0333 bits (201), Expect = 6.364e-15
Identity = 86/283 (30.39%), Postives = 123/283 (43.46%), Query Frame = 3
Query: 2598 IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDS-KICSGNGI-CQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG----------LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            I D  +D+NEC  +   CHN   CINT GSF C C            C   ++EC+      +            C++  G   C C  GF       C DINEC  NP L    C N++G ++C CP G+        C D+DEC +    C   G+ C+N+IG++ C C  G    ++D+ C D +EC+    +C+  N  C NT GS+ C C  G          L+Y++  C    E  Q   C   S+   +     C C+ G  + N  + C L  T
Sbjct: 2019 IDDTSVDVNECVENPGICHN-GHCINTDGSFRCECPMGYTLNYTGVHCV-DVDECAVGNPCGN----------GTCTNVIGGFECNCEEGFEPGPMMACEDINECGQNPLLCAFRCINTFGGYECSCPSGYTLREDGRMCQDLDECVEGLHDCDTRGMTCKNLIGTFMCICPPGMVRRMDDELCQDENECRTKPGICE--NGRCVNTIGSYMCECGEGFQPDPTHTQCLDYRQGFC--FTEVLQ-TMCQMSSSSRTAITKSQCCCDGGHGWGNQCELCPLPGT 2284          

HSP 13 Score: 80.1073 bits (196), Expect = 2.101e-14
Identity = 75/247 (30.36%), Postives = 98/247 (39.68%), Query Frame = 3
Query: 2574 NCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGD---------------------NVVCQNTPGSFKCVCPVG 3233
            +C  GY     G  C+D++EC       N  C NT GS+ C C            C   ++EC  +R                C ++ G   C CP GFT      CID NEC   P  C  K  C+NS GSF C C +GF        C D++EC  +  C     C+NM+G YRC C  GY ++ +   C+D +EC                               N  C NT G + C CP G
Sbjct: 2329 HCNSGYTASIAGTACVDLDECAMSPKPCNFICKNTEGSYACSCPRGYILQEDSRSCK-DLDECQVKRHNCQF----------MCVNTIGGFTCKCPPGFTQH-HTACIDHNECAAEPSLCGAKGLCQNSPGSFSCECQRGFSLDTSGLHCEDVNECDSNHRCQFG--CENMLGGYRCNCPQGYTQHYQWNQCVDENECSTPGTCGSASCYNTLGSFKCAYECTSFKNPCNYGCSNTQGGYLCGCPPG 2561          

HSP 14 Score: 76.6406 bits (187), Expect = 2.291e-13
Identity = 70/225 (31.11%), Postives = 108/225 (48.00%), Query Frame = 3
Query: 2817 CSSSNGVEF-CVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTC----PEGSTCINSFGGF---NCTCNKGFVFNNDTKFCELDKTL--NVVQPKADLVFGIDTSE 3452
            C + +  EF  +CP G  Y +     DINEC M+P  C + A C N  G + C C  G++ +   G   +DEC  +++   NG+C+N+ GSY C C  GY Y +D + C D++EC++S  +    N +C+N  GSF C C  G +    G + ID      TC     EG   +N  GG     C    G  + +  + CE+D        +P+  +   ++  E
Sbjct:  599 CPTLDSAEFNALCPSGTGYAIDG--TDINECAMDPDICPNGA-CENLRGGYRCICNIGYE-TDQSGRSYVDECFVNRLLCDNGLCRNVPGSYTCTCPKGYSYRQDSETCEDVNECESSPCI----NGLCKNNAGSFACECSPGSKVDPTGLICID--SLKGTCWLTIQEGRCEVNINGGTLRSECCATLGAAWGSPCEPCEIDPVCQRGFARPRGTICEDVNECE 813          

HSP 15 Score: 74.7146 bits (182), Expect = 9.176e-13
Identity = 102/354 (28.81%), Postives = 141/354 (39.83%), Query Frame = 3
Query: 2526 LSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEECSFERKIISICDAVTC---------RSPANCS----SSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDE------------------------CQASKKLCQGDNVVCQNTPG----SFKCVC-------PVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            L ++L   F   C +   G    + G NC D++EC D + C N   C+NTPGS+ C C  + E LNP   GC  P++  C F   +      + C         RS   CS      N  E C          +CP G  +      I   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC  +    G G C N +G+Y C C   Y  V     C+D+ +                        C  +  + +  N  CQ  P      F+ +C        + +E  +   VDIDEC +         CIN  G F C C +GF +N+    CE
Sbjct: 1358 LCENLPGAFRCVCDE---GYELNRSGINCTDVDECLDPINCVN-GHCVNTPGSYECNCPTDFE-LNPTGIGCVDPRVGFC-FLEAVPRGRGGLACSVEVGVGVSRSSCCCSLGRAWGNPCEGCPPVNTSDYNTLCPGGEGFRPNPITIILEDIDECQELPGLCQGGNCVNTFGSFQCDCPGGYYLNADSRICEDIDECVVNPGICGPGTCYNTLGNYTCVCPQEYMQVNGGNNCMDMRKSLCFRNYNGTACENELAFNITHRICCCAYNMGKAWNKPCQACPRPGTEGFRTLCGNIVPGFEIDIETGKP--VDIDECNEIPGICASGVCINQIGSFRCECPRGFSYNDVLLICE 1703          

HSP 16 Score: 69.707 bits (169), Expect = 3.590e-11
Identity = 42/100 (42.00%), Postives = 51/100 (51.00%), Query Frame = 3
Query: 3093 CHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+   +    C D+DECQA   LCQG N V  NT GS++C CP G    E    C D+DEC       EG  C NS G F+C C  G+V + D   C
Sbjct:  172 CRRGFIPNIRTGACQDVDECQAVPGLCQGGNCV--NTVGSYECKCPAGHRLNEVTNKCEDVDECSTVQHVCEGGQCTNSVGSFSCVCPSGYVLSADKTRC 269          

HSP 17 Score: 67.3958 bits (163), Expect = 1.500e-10
Identity = 94/330 (28.48%), Postives = 134/330 (40.61%), Query Frame = 3
Query: 2526 LSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNP---------EFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFC----VCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDI-----------DEC--------RDSKICSGNGIC---------QNMIGSYRCACHNGYEYVEDKGCI-------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            L +++   +  TC     G  Y++  + C D+NEC+S  C  N  C N  GSF C CS   + ++P         +  C   I+E   E  I        C +    +  +  E C    VC RGF       C D+NEC + P +     C N+ GSF C C +G    S    CVDI           DEC        R    C   G           +     Y   C  G  +      +       DI+EC+    +C   +  C+NT GSF+C C  G  L+ +E+ C DIDEC+   + C  G+ C+N+ G F C C  G+
Sbjct:  678 LCRNVPGSYTCTCPK---GYSYRQDSETCEDVNECESSPCI-NGLCKNNAGSFACECSPGSK-VDPTGLICIDSLKGTCWLTIQEGRCEVNINGGTLRSECCATLGAAWGSPCEPCEIDPVCQRGFARPRGTICEDVNECEVFPGVCPNGRCVNTRGSFKCQCSEGLTLDSTGRICVDIRLEHCYLTYDEDECVQPVSGRFRMDACCCTVGAAWGKECEACPEPGTREYEALCPRGPGFANRGDVLTGRPVYKDINECKVFPSICA--HGTCRNTIGSFRCRCNGGFALDAEERNCTDIDECKISPDLCGHGA-CVNTPGSFECDCLPGY 999          

HSP 18 Score: 58.9214 bits (141), Expect = 5.676e-8
Identity = 87/280 (31.07%), Postives = 110/280 (39.29%), Query Frame = 3
Query: 2652 NSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVH--FGC---------VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDE--------------------------CQASKKLCQGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGF 3356
            N RCI    S  C C  N  +     G    I+EC        + D  T     +C ++ G  +C C  GF     K+ C D +EC + N CL+   C N  GSF C C  GF  +    F C         V IDEC    IC  +G C N  GSYRC C  G     D + C+D                             C AS     G+   CQ  P      F  +CP G  Y   G  DI+EC  + + CP G+ C N  GG+ C CN G+
Sbjct:  394 NGRCILVGSSHRCEC--NAGFRVDSLGECSDIDEC--------VSDPCT---NGDCVNTAGSFYCKCHTGFHRPPGKQACFDHDECTSTNMCLN-GMCINEEGSFKCVCKHGFTLASKKCFECYMSAIWLFFVYIDECLTPGICL-HGRCINTEGSYRCECLAGLAVGPDGRSCVDTHMRSTCYGAIKKGVCVRPFQAAVTRSACCCASASYSFGE--PCQPCPTLDSAEFNALCPSGTGYAIDG-TDINECAMDPDICPNGA-CENLRGGYRCICNIGY 654          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Match: fbn2b (fibrillin 2b [Source:ZFIN;Acc:ZDB-GENE-090112-3])

HSP 1 Score: 131.339 bits (329), Expect = 5.712e-30
Identity = 107/301 (35.55%), Postives = 149/301 (49.50%), Query Frame = 3
Query: 2523 ELSQHLSK----VFDITCKDVNCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEY-LNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKG-FQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            ELS +L +    V  +     +C  GYQ   D   C+DI+EC  +    ++ C N+ GS+ C CS  E Y L P+      I+EC        ICD         C++  G   C+C  GF  T D++  C D+NEC++NP  CL    C N+ GSF C C  G F      GC D+DEC   +  C  +  C N+ GS++C C +G+   +   C+D+DEC   +  C   N  C NTPGS++C C  G       C D+DEC +N    E   C+N+ GG+ C C  GF    DTK C+
Sbjct: 1092 ELSDNLCRNGQCVNMVGSYQCSCDTGYQVTPDRQGCVDIDECTIMNGGCDTHCTNSEGSYECSCS--EGYALMPDLRTCADIDEC---EDTPDICDG------GQCTNIPGEYRCLCFDGFMSTMDMRT-CEDVNECDLNPNICL-HGDCENTKGSFICHCQLGYFVKKGSTGCTDVDECEIGAHNCDMHAACVNVPGSFKCRCRDGW-VGDGIKCVDLDECSTEEHSCN-PNANCVNTPGSYRCACKEGFNGDGFSCSDMDECAENVNLCENGQCLNTPGGYRCECEMGFTPTEDTKACQ 1377          

HSP 2 Score: 119.783 bits (299), Expect = 2.052e-26
Identity = 95/269 (35.32%), Postives = 133/269 (49.44%), Query Frame = 3
Query: 2574 NCGKGYQK--IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            +C  GY     G +CID++EC       N  C NT GS++C C      L P+      ++ECS ++                C ++ G   C CP GFT   +  CID NEC   P  C  +A+C N+ GSF+C C KGF        C D+DEC  +  C     CQNM+G +RC C  GY ++ +   C+D +EC  ++      +  C NT GS+KCVCP G +++    GC DI+EC   +  P    C N+ GG+ C C  GF
Sbjct: 2328 HCNVGYTNDFSGTSCIDLDECSQSPKPCNFLCKNTEGSYLCSCPRGY-ILQPDGKTCKDLDECSTKQHNCQF----------LCVNTIGGFTCKCPSGFTQH-QTACIDNNECTNQPSVCGSRASCLNTPGSFNCECQKGFSLDGTGVECEDVDECTGNHRCQHG--CQNMLGGFRCGCPQGYVQHYQWNQCVDENECSGNQVC---GSASCYNTLGSYKCVCPSGFDFQAGAGGCQDINECSMGSN-PCSFGCSNTDGGYLCGCPGGF 2578          

HSP 3 Score: 119.398 bits (298), Expect = 2.850e-26
Identity = 94/267 (35.21%), Postives = 131/267 (49.06%), Query Frame = 3
Query: 2616 DINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            D+NEC     L  H   R  NT GSF C C+N       E  C   I+EC+    +              C ++ G   C C  G+   + + + C+DI+EC  +P L +  TC N+ GS++C CP+G Q S     C D++EC  S     NG C NM+GSY+C+C  GY+   D+ GC+DIDEC      C   +  C N+ GS++C C  G  L    + C DIDEC+      +G  C N  G + C C  GF+   D + CE
Sbjct:  960 DVNECKVFKGLCTHGTCR--NTIGSFKCRCNNGFALTMEERNCT-DIDECAISPDLCG---------QGTCVNTPGSFECECFEGYESGFMMMKNCMDIDECERDPLLCRGGTCLNTEGSYECECPRGHQLSPGGSSCEDVNECELSDNLCRNGQCVNMVGSYQCSCDTGYQVTPDRQGCVDIDECTIMNGGC---DTHCTNSEGSYECSCSEGYALMPDLRTCADIDECEDTPDICDGGQCTNIPGEYRCLCFDGFMSTMDMRTCE 1211          

HSP 4 Score: 115.931 bits (289), Expect = 3.331e-25
Identity = 76/184 (41.30%), Postives = 103/184 (55.98%), Query Frame = 3
Query: 2844 CVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C C  G+  D++  CID++EC  NPC++   C N+ GS+ C C +G+Q  +    C+DIDEC  + +   NG C N  GS++C C+ G++   D K CID DEC A+  +C   N +C N  GSFKC+C  G      G  C DIDECQ    C  G  CINS G F+C C  G   + D + C
Sbjct:  406 CECNMGYRQDMRGECIDVDECISNPCVN-GDCVNTQGSYHCKCHEGYQGTATKQACIDIDECIVNGVMCRNGRCVNTDGSFQCICNAGFDLSPDGKNCIDHDEC-ATTNMCL--NGMCINEDGSFKCICKQGFALAPNGRYCTDIDECQTPGICMNGR-CINSEGSFHCECPPGLAIDVDGRVC 584          

HSP 5 Score: 113.235 bits (282), Expect = 1.887e-24
Identity = 103/291 (35.40%), Postives = 139/291 (47.77%), Query Frame = 3
Query: 2601 GDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISIC-------DAVTCRSPANCSSSNG----VEFC----------VCP--RGFTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G+ C+D+NEC +      N RC+NT GS+ C CS   E       C    +   F   + ++C       ++VT +S   C+   G     E C          +CP   G+T D +    DINEC + P L K   C NS GSF C C  G+        C+D+DEC  S K C  N +C+N  GSY C+C  GY    D K C D+DEC   +  CQ    +C NT G F C CP G    +  C+D +EC  Q + C   ++C+N+ G FNC C KGF  +     CE
Sbjct: 2186 GEGCMDLNECRTKPGLCKNGRCVNTVGSYRCECSEGFEPSATGTECIDNRQGFCFTEVLQTMCQQGSTNRNSVT-KSECCCNGGRGWGPQCELCPLPGTVQYKKMCPLGPGYTTDGR----DINECEVLPNLCKNGQCINSVGSFRCHCNVGYTNDFSGTSCIDLDECSQSPKPC--NFLCKNTEGSYLCSCPRGYILQPDGKTCKDLDECSTKQHNCQ---FLCVNTIGGFTCKCPSGFTQHQTACIDNNECTNQPSVCGSRASCLNTPGSFNCECQKGFSLDGTGVECE 2466          

HSP 6 Score: 109.768 bits (273), Expect = 2.588e-23
Identity = 95/275 (34.55%), Postives = 135/275 (49.09%), Query Frame = 3
Query: 2613 IDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVD-IDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNV 3407
            IDI+EC  +     N  CIN  GSF C C     Y N    C   I+EC+    +        C+  ANC +  G   C C  GF       C+D NEC   P +     C +  GS+ C C  GF+ +     C+D+DEC     ++C  +G C N +GS++C C  GYE   D K C D++EC      C      CQN  GSF+C+CP G  ++  +  CV  I++C+ + N C  G TCIN+ G F C C  GFV +++ + C    T+++
Sbjct: 1694 IDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICE-DIDECNSGDNL--------CQRNANCINIPGSYRCECSPGFKLSPSGACVDRNECKEKPNVCSHGDCIDMQGSYRCLCHNGFKATADQTMCMDVDECTTLSGQVCR-HGQCINSLGSFQCLCQEGYELTPDNKNCADVNECVTFPGTCSPG--TCQNLVGSFRCICPPGYAVQNDQLTCVVYINDCEVEPNICQFG-TCINTPGSFQCICQPGFVLSDNGRRCFGKFTVDI 1955          

HSP 7 Score: 107.457 bits (267), Expect = 1.148e-22
Identity = 95/279 (34.05%), Postives = 128/279 (45.88%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-PKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            G GY   G    DINEC+ L     N +CIN+ GSF C+C  N  Y N   G +   ++ECS   K            P N  C ++ G   C CPRG+      + C D++EC+      +  C N+ G F C CP GF       C+D +EC +   +C     C N  GS+ C C  G+  ++  G  C D+DEC  + +   G    CQN  G F+C CP G    Y+   CVD +EC  N  C   S C N+ G + C C  GF F      C+
Sbjct: 2293 GPGYTTDGR---DINECEVLPNLCKNGQCINSVGSFRCHC--NVGYTNDFSGTSCIDLDECSQSPK------------PCNFLCKNTEGSYLCSCPRGYILQPDGKTCKDLDECSTKQHNCQFLCVNTIGGFTCKCPSGFTQH-QTACIDNNECTNQPSVCGSRASCLNTPGSFNCECQKGFS-LDGTGVECEDVDECTGNHRCQHG----CQNMLGGFRCGCPQGYVQHYQWNQCVDENECSGNQVCGSAS-CYNTLGSYKCVCPSGFDFQAGAGGCQ 2547          

HSP 8 Score: 102.834 bits (255), Expect = 2.564e-21
Identity = 103/311 (33.12%), Postives = 140/311 (45.02%), Query Frame = 3
Query: 2601 GDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDS-KICSGNGI-CQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-------NNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKN 3497
            GD   D+NEC D+     N  CINT GSF C C            C    +ECS    I + C   TC +        G E C C  GF       C DINEC++NP L    C N++GS++C CP G+        C D DEC +    C+  G+ C+N+IG++ C C  G     D +GC+D++EC+    LC+  N  C NT GS++C C  G E    G   ID  Q           C +GST  NS     C CN G  +    + C L  T+   +    L  G  T     N  +++ ++ KN
Sbjct: 2019 GDGREDMNECLDNPGICVNGMCINTDGSFRCECPFGYNLDYTGVNCV-DTDECS----IGNPCGNGTCTNVVG-----GFE-CSCQEGFEPGPMMTCEDINECSLNPLLCAFRCVNTFGSYECMCPAGYVLRDDQRMCRDQDECTEGLDDCASRGMACKNLIGTFMCICPPGMTRRPDGEGCMDLNECRTKPGLCK--NGRCVNTVGSYRCECSEGFEPSATGTECIDNRQGFCFTEVLQTMCQQGSTNRNSVTKSECCCNGGRGWGPQCELCPLPGTVQ-YKKMCPLGPGYTTDGRDINECEVLPNLCKN 2315          

HSP 9 Score: 98.5969 bits (244), Expect = 5.217e-20
Identity = 91/310 (29.35%), Postives = 139/310 (44.84%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDID----------------ECQASKKLCQGDNVVCQNTPGS---------------FKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+   G  C+D++EC  +  +C+ N+ C+NTPGS+ C C   E +    F C+  ++EC+   + +++C+         C ++ G   C C  GFT  +  + C DI+ECN        +C+N  G F C C  G++     G C D++EC D   C  NG+C NM GSY C C   +E      GC+D                   C A   +       C +  G+               +K +CP G  ++         DIDECQ+     +G  C+N+FG F C C  G+  N +T+ CE
Sbjct: 1282 CRDGWVGDGIKCVDLDECSTEEHSCNPNANCVNTPGSYRCACK--EGFNGDGFSCS-DMDECA---ENVNLCEN------GQCLNTPGGYRCECEMGFTPTEDTKACQDIDECNFQNICVFGSCQNLPGMFRCVCDDGYELDRSGGNCTDVNECADPVNCI-NGLCVNMPGSYLCNCPADFELNPTGVGCVDTRVGNCFLDTLDRGDGGISCSAEIGVGVTRASCCCSLGGAWGNPCELCPPVNSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICE 1578          

HSP 10 Score: 97.4413 bits (241), Expect = 1.252e-19
Identity = 101/313 (32.27%), Postives = 133/313 (42.49%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSP-----ANCSSSNGVEFCVCPRGFTYDLKE-------------GCIDINECNM---------------------NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            N G      G NCID +EC +     N  CIN  GSF C C          F  AP    C+         D   C++P       C +S G   C CP G   D+               G I +  C+                       PC      RNS   F   C  G   +      DI+EC  D +IC  NG+C+N++GSYRC C+ GYE     K C+DI+EC  ++ LC  DN +C+NTPGSF C CP G  +K   + C DI+EC +++ C  G  C N  G F C C+ G   ++    C
Sbjct:  490 NAGFDLSPDGKNCIDHDECATTNMCLNGMCINEDGSFKCICKQG-------FALAPNGRYCT---------DIDECQTPGICMNGRCINSEGSFHCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGTCSRPFPGAVTKSECCCANPEHGFGQPC-QPCPARNS-AEFQAVCSSGIGITTDG--RDINECALDPEICQ-NGMCENLLGSYRCICNIGYESDSSGKNCVDINECLVNRLLC--DNGLCRNTPGSFTCSCPKGFVFKADSETCEDINEC-ESSPCVNG-ICRNLAGSFTCECSHGSKLDSTNTVC 777          

HSP 11 Score: 93.9745 bits (232), Expect = 1.343e-18
Identity = 94/308 (30.52%), Postives = 134/308 (43.51%), Query Frame = 3
Query: 2577 CGKGYQKIG--DNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-------------FGCVDIDECR--------DSKICSGN---GICQ-------NMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GYQ       CIDI+EC  + + C N  RC+NT GSF C C+   + L+P+       +EC+     ++            C + +G   C+C +GF        C DI+EC          C NS GSF C CP G    V              +G + +  C          S+ C  N   G  Q            ++  C +G     D    DI+EC    ++CQ  N +C+N  GS++C+C +G E    G  CVDI+EC  N    +   C N+ G F C+C KGFVF  D++ CE
Sbjct:  447 CHEGYQGTATKQACIDIDECIVNGVMCRN-GRCVNTDGSFQCICNAGFD-LSPDGKNCIDHDECATTNMCLN----------GMCINEDGSFKCICKQGFALAPNGRYCTDIDECQTPGICMNGRCINSEGSFHCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGTCSRPFPGAVTKSECCCANPEHGFGQPCQPCPARNSAEFQAVCSSGIGITTDG--RDINECALDPEICQ--NGMCENLLGSYRCICNIGYESDSSGKNCVDINECLVNRLLCDNGLCRNTPGSFTCSCPKGFVFKADSETCE 738          

HSP 12 Score: 80.4925 bits (197), Expect = 1.647e-14
Identity = 94/302 (31.13%), Postives = 130/302 (43.05%), Query Frame = 3
Query: 2577 CGKGY-QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDID---ECQASKKLC---------------------QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY Q +   CID++EC S  C N   C+NT GS+ C C    +    +  C   I+EC        I + V CR+   C +++G   C+C  GF      + CID +EC   N CL+   C N  GSF C C +GF  + +   C DIDEC+   IC  NG C N  GS+ C C  G    V+ + C+D      C  + K+                       G    CQ  P      F+ VC  G+     G  DI+EC  +    +   C N  G + C CN G+  ++  K C
Sbjct:  408 CNMGYRQDMRGECIDVDECISNPCVNGD-CVNTQGSYHCKCHEGYQGTATKQACI-DIDEC--------IVNGVMCRN-GRCVNTDGSFQCICNAGFDLSPDGKNCIDHDECATTNMCLN-GMCINEDGSFKCICKQGFALAPNGRYCTDIDECQTPGICM-NGRCINSEGSFHCECPPGLAIDVDGRVCVDTHMRTTCYGAIKMGTCSRPFPGAVTKSECCCANPEHGFGQPCQPCPARNSAEFQAVCSSGIGITTDG-RDINECALDPEICQNGMCENLLGSYRCICNIGYESDSSGKNC 695          

HSP 13 Score: 77.7962 bits (190), Expect = 1.272e-13
Identity = 74/234 (31.62%), Postives = 100/234 (42.74%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   +T K+L      QH      ++ +   TCK   C  G+ +    CID NEC +    C + + C+NTPGSF C C            C   ++EC+              R    C +  G   C CP+G+    +   C+D NEC+ N     A+C N+ GS+ C CP GF +     GC DI+EC   S  CS    C N  G Y C C  G+       CI
Sbjct: 2371 PRGYILQPDGKTCKDLDECSTKQHNCQFLCVNTIGGFTCK---CPSGFTQHQTACIDNNECTNQPSVCGSRASCLNTPGSFNCECQKGFSLDGTGVECE-DVDECTGNH-----------RCQHGCQNMLGGFRCGCPQGYVQHYQWNQCVDENECSGNQVCGSASCYNTLGSYKCVCPSGFDFQAGAGGCQDINECSMGSNPCSFG--CSNTDGGYLCGCPGGFYRAGQGHCI 2587          

HSP 14 Score: 75.485 bits (184), Expect = 5.635e-13
Identity = 99/347 (28.53%), Postives = 139/347 (40.06%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCA-PKIEEC---SFERKIISI-CDA--------VTCRSPANCSSSNGVEFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDI----------DECQ------ASKKLC-------QGDNVVCQNTP----GSFKCVCP-----VGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            Q+L  +F   C D   G    + G NC D+NEC       N  C+N PGS++C C  + E LNP   GC   ++  C   + +R    I C A         +C      +  N  E C          +CP G  +      +   DI+EC   P L     C N++GSF C CP G+  +     C DIDEC       G G C N +G+Y C C   Y  V     C+D+          D C+       +KK+C       +  N  C+  P      ++ +C        ++      +DIDEC++         CIN  G F C C  GF +NN    CE
Sbjct: 1394 QNLPGMFRCVCDD---GYELDRSGGNCTDVNECADPVNCINGLCVNMPGSYLCNCPADFE-LNPTGVGCVDTRVGNCFLDTLDRGDGGISCSAEIGVGVTRASCCCSLGGAWGNPCELCPPVNSTEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPAGYYLNEETRICEDIDECTAHIGICGPGTCYNTLGNYTCVCPPEYMQVNGGNNCMDMRKSVCYRNFNDTCENELSFNMTKKMCCCAYNVGKAWNKPCEACPTPATSEYQLLCGNQAPGFIIDIHTGKPIDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICE 1736          

HSP 15 Score: 70.8626 bits (172), Expect = 1.365e-11
Identity = 87/306 (28.43%), Postives = 113/306 (36.93%), Query Frame = 3
Query: 2610 CIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CID--INEC------------------NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD------------------------IDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE--YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C D++EC ++        CINT GS+ C C            C   I+EC+    +  +CD         C+++ G   C CPRG+        CID  I  C                   M  C D   C  + G     CP      +     D                        ID C+       NG C     SYRC C+ GY       CID+DEC  S     GD   C NT GS+ C C  G +    ++ C+DIDEC  N        C+N+ G F C CN GF  + D K C
Sbjct:  213 CQDVDECQAIPGLCAGGNCINTVGSYECKCPAGHRQSETNQKCE-DIDECA----LPGLCDG------GECTNTAGSYVCTCPRGYVSSADGSRCIDQRIGTCFSALANGRCAAELTGQFSKMQCCCDLGRCW-ALGHIPEMCPVRGSGELFAKACDSPQSYASSPSPSPYPPELLLLHSTIDVCKHFTNLCLNGRCIPTTASYRCECNMGYRQDMRGECIDVDEC-ISNPCVNGD---CVNTQGSYHCKCHEGYQGTATKQACIDIDECIVNGVMCRNGRCVNTDGSFQCICNAGFDLSPDGKNC 502          

HSP 16 Score: 67.0106 bits (162), Expect = 2.246e-10
Identity = 89/324 (27.47%), Postives = 124/324 (38.27%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVE----FCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCID------------------------IDECQASKKLCQGDNVVCQNTPGS--FKCVCPVGLEYKEKGCV--------DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G NC+DINEC  + L C +N  C NTPGSF C C     +      C   I EC     +  IC  +       CS  + ++     CV     T  L    I  N C  N+N    K+ C ++ G+           D  CP+G        C DI+EC        NG+C N  GS+RC C  G    E  + C+D                        +D C  S     G        PG+  ++ +CP G  +  +G +        D++EC+         TC N+ G F C CN GF    + + C
Sbjct:  682 CNIGYESDSSGKNCVDINECLVNRLLC-DNGLCRNTPGSFTCSCPKGFVFKADSETCE-DINECESSPCVNGICRNLAGSFTCECSHGSKLDSTNTVCVDSMKGTCWLN---IQDNRCEVNINGATLKSECCSTLGAAWGSPCERCEVDTACPRGLARMKGLICEDINECEVFPGVCSNGVCVNTHGSFRCECAEGLTLDEGGRTCVDVRSEHCYMKWDEDECGEPLPGRYRVDMCCCSVGAAWGLECESCPKPGTPEYRTICPRGPGFANRGDILTGRPFYKDVNECKVFKGLCTHGTCRNTIGSFKCRCNNGFALTMEERNC 1000          

HSP 17 Score: 64.3142 bits (155), Expect = 1.620e-9
Identity = 41/100 (41.00%), Postives = 53/100 (53.00%), Query Frame = 3
Query: 3093 CHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  G+   +    C D+DECQA   LC G N  C NT GS++C CP G    E  + C DIDEC     C +G  C N+ G + CTC +G+V + D   C
Sbjct:  200 CRRGFIPNIRSGACQDVDECQAIPGLCAGGN--CINTVGSYECKCPAGHRQSETNQKCEDIDECALPGLC-DGGECTNTAGSYVCTCPRGYVSSADGSRC 296          

HSP 18 Score: 56.9954 bits (136), Expect = 2.600e-7
Identity = 43/104 (41.35%), Postives = 56/104 (53.85%), Query Frame = 3
Query: 2970 CPKGFQYSVHFG-CVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDE 3272
            C +GF  ++  G C D+DEC+    +C+G G C N +GSY C C  G+   E ++ C DIDEC A   LC G    C NT GS+ C CP G      G   ID+
Sbjct:  200 CRRGFIPNIRSGACQDVDECQAIPGLCAG-GNCINTVGSYECKCPAGHRQSETNQKCEDIDEC-ALPGLCDGGE--CTNTAGSYVCTCPRGYVSSADGSRCIDQ 299          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000047922.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:72873715:72914547:-1 gene:ENSAMXG00000010096.2 transcript:ENSAMXT00000047922.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.487 bits (319), Expect = 6.562e-29
Identity = 92/298 (30.87%), Postives = 125/298 (41.95%), Query Frame = 3
Query: 2595 KIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-------KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNN 3368
             I + C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+DEC  +  +C G N +C NT G + C C  G               C D+DEC+   + C   S C N+ GG+NCTC  GF+  N
Sbjct:  310 NISNPCSDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTN 598          

HSP 2 Score: 124.405 bits (311), Expect = 6.347e-28
Identity = 93/295 (31.53%), Postives = 127/295 (43.05%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLK----EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGY-------EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT    DL       C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C C  G+              C D+DEC+ +  +C G N +C NT G + C C  G               C D+D+C    + C   STC+N+ GG+NC+C KGF   N +
Sbjct:  413 CTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSICGPNSTCLNTKGGYNCSCLKGFTALNSS 698          

HSP 3 Score: 119.398 bits (298), Expect = 1.907e-26
Identity = 91/292 (31.16%), Postives = 126/292 (43.15%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYC------SNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--------GCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNN 3368
            D++EC   + AC  NS C NT G + C C      +N+   +N    C+  ++EC+    I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC  +  IC  N IC N IG Y C+C  G+              C D+DEC+ +  +C G N +C NT G + C C  G               C D+DEC    + C   S C N+ GG+NC+C  GF   N
Sbjct:  268 DVDECVLGTSACGPNSICSNTIGGYNCSCRTGFIVTNSSLPVNISNPCS-DVDECATAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATN 549          

HSP 4 Score: 117.857 bits (294), Expect = 6.618e-26
Identity = 87/290 (30.00%), Postives = 121/290 (41.72%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFV 3359
            C D++EC      C  NS C NT G + C C       NP+   +       ++EC+    I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+D+C  +  +C G N  C NT G + C C  G               C+D+DEC      C + + C N+ GG+NC+C  GF 
Sbjct:  462 CTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSIC-GPNSTCLNTKGGYNCSCLKGFTALNSSLPVNISNPCIDVDECLFTQLYCGQNTNCTNTIGGYNCSCWLGFT 742          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000035254.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:72873715:72914547:-1 gene:ENSAMXG00000010096.2 transcript:ENSAMXT00000035254.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.487 bits (319), Expect = 6.562e-29
Identity = 92/298 (30.87%), Postives = 125/298 (41.95%), Query Frame = 3
Query: 2595 KIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-------KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNN 3368
             I + C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+DEC  +  +C G N +C NT G + C C  G               C D+DEC+   + C   S C N+ GG+NCTC  GF+  N
Sbjct:  310 NISNPCSDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTN 598          

HSP 2 Score: 124.405 bits (311), Expect = 6.347e-28
Identity = 93/295 (31.53%), Postives = 127/295 (43.05%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLK----EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGY-------EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT    DL       C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C C  G+              C D+DEC+ +  +C G N +C NT G + C C  G               C D+D+C    + C   STC+N+ GG+NC+C KGF   N +
Sbjct:  413 CTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSICGPNSTCLNTKGGYNCSCLKGFTALNSS 698          

HSP 3 Score: 119.398 bits (298), Expect = 1.907e-26
Identity = 91/292 (31.16%), Postives = 126/292 (43.15%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYC------SNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--------GCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNN 3368
            D++EC   + AC  NS C NT G + C C      +N+   +N    C+  ++EC+    I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC  +  IC  N IC N IG Y C+C  G+              C D+DEC+ +  +C G N +C NT G + C C  G               C D+DEC    + C   S C N+ GG+NC+C  GF   N
Sbjct:  268 DVDECVLGTSACGPNSICSNTIGGYNCSCRTGFIVTNSSLPVNISNPCS-DVDECATAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATN 549          

HSP 4 Score: 117.857 bits (294), Expect = 6.618e-26
Identity = 87/290 (30.00%), Postives = 121/290 (41.72%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFV 3359
            C D++EC      C  NS C NT G + C C       NP+   +       ++EC+    I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+D+C  +  +C G N  C NT G + C C  G               C+D+DEC      C + + C N+ GG+NC+C  GF 
Sbjct:  462 CTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSIC-GPNSTCLNTKGGYNCSCLKGFTALNSSLPVNISNPCIDVDECLFTQLYCGQNTNCTNTIGGYNCSCWLGFT 742          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000010372.2 (pep primary_assembly:Astyanax_mexicanus-2.0:3:72873715:72914547:-1 gene:ENSAMXG00000010096.2 transcript:ENSAMXT00000010372.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.102 bits (318), Expect = 8.666e-29
Identity = 92/298 (30.87%), Postives = 125/298 (41.95%), Query Frame = 3
Query: 2595 KIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-------KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNN 3368
             I + C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+DEC  +  +C G N +C NT G + C C  G               C D+DEC+   + C   S C N+ GG+NCTC  GF+  N
Sbjct:  359 NISNPCSDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTN 647          

HSP 2 Score: 124.02 bits (310), Expect = 8.661e-28
Identity = 93/295 (31.53%), Postives = 127/295 (43.05%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY---DLK----EGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGY-------EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C D++EC +    C  NS C NT G + C C       NP    +       ++EC     I        C   + CS++ G   C C  GFT    DL       C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C C  G+              C D+DEC+ +  +C G N +C NT G + C C  G               C D+D+C    + C   STC+N+ GG+NC+C KGF   N +
Sbjct:  462 CTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSIC-GPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSICGPNSTCLNTKGGYNCSCLKGFTALNSS 747          

HSP 3 Score: 119.398 bits (298), Expect = 1.868e-26
Identity = 93/312 (29.81%), Postives = 133/312 (42.63%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYC------SNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-------TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADL 3428
            D++EC   + AC  NS C NT G + C C      +N+   +N    C+  ++EC        +     C   + CS++ G   C C  GF         ++   C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+DEC  +  +C G N +C NT G + C C  G               C D+DEC+   + C   S C N+ GG+NC+C  GF   N       D  L+   P  D+
Sbjct:  268 DVDECVLGTSACGPNSICSNTIGGYNCSCRTGFIVTNSSLPVNISNPCS-DVDEC--------VLGTSACGPNSICSNTIGGYNCSCRTGFIVTNSSLPVNISNPCSDVDECATAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTANPCTDVDECATAPSIC-GPNSICSNTIGGYNCSCRTGFTVTNPALNLSTTNLCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNP------DLNLSTTNPCTDV 563          

HSP 4 Score: 117.087 bits (292), Expect = 9.641e-26
Identity = 87/290 (30.00%), Postives = 121/290 (41.72%), Query Frame = 3
Query: 2610 CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-----IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-------YDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGF-------QYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDK-------GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFV 3359
            C D++EC      C  NS C NT G + C C       NP+   +       ++EC+    I        C   + CS++ G   C C  GFT             C D++EC   P  C   + C N+ G ++C C  GF         S    C D+DEC+ +  IC  N IC N IG Y C+C  G+              C D+D+C  +  +C G N  C NT G + C C  G               C+D+DEC      C + + C N+ GG+NC+C  GF 
Sbjct:  511 CTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPDLNLSTTNPCTDVDECATAPSI--------CGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDECKPAPSICGPNSICSNTIGGYNCTCRTGFIVTNPALNLSTANPCTDVDECKPAPSICGPNSICSNTIGGYNCSCRTGFTATNPALNLSTTNPCTDVDKCLTTPSIC-GPNSTCLNTKGGYNCSCLKGFTALNSSLPVNISNPCIDVDECLFTQLYCGQNTNCTNTIGGYNCSCWLGFT 791          

HSP 5 Score: 58.5362 bits (140), Expect = 7.558e-8
Identity = 39/116 (33.62%), Postives = 52/116 (44.83%), Query Frame = 3
Query: 3054 GICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--------CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            G+C   IG   C C  G   V++    D+DEC      C G N +C NT G + C C  G               C D+DEC    + C   S C N+ GG+NC+C  GF+  N +
Sbjct:  243 GMCDTSIGG-TCGCIRGLP-VDEMCQRDVDECVLGTSAC-GPNSICSNTIGGYNCSCRTGFIVTNSSLPVNISNPCSDVDECVLGTSACGPNSICSNTIGGYNCSCRTGFIVTNSS 355          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002704.1 (pep scaffold:Pmarinus_7.0:GL476354:513769:588114:-1 gene:ENSPMAG00000002318.1 transcript:ENSPMAT00000002704.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 159.073 bits (401), Expect = 5.194e-39
Identity = 108/272 (39.71%), Postives = 147/272 (54.04%), Query Frame = 3
Query: 2589 YQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            Y  I   C DINEC S    C  N+ CIN+ GS+ C CS    Y    FG     +ECS + +I S         P  C  + G   C+CP G+   + +  C+D++EC   PC    TCRN+ GSF+C C  GF+ S +  C+D DEC    + +C   G C N +GS++C C+ G+E   DK  C DI+ECQ  + +C      CQN  GSF+C+CP G E KE+ C DIDEC  + + C  GS C+N+FG F C C  GF+ +   + C
Sbjct: 1739 YNDIQIICEDINECASAEKMCQRNAECINSVGSYRCECSPG--YTQTPFGLCIDRDECSEKPEICS---------PGRCQDTEGSYRCMCPNGYKATEDQNMCMDVDECERQPC-GNGTCRNTVGSFNCLCFPGFEISANNDCMDKDECVSLAAHVCK-FGSCINNVGSFKCLCNEGFELTADKKNCRDINECQQFRGMCAPG--TCQNLEGSFRCMCPPGYEVKEEACTDIDECSSEEDLCMMGS-CLNTFGSFECRCPSGFILSETGRKC 1994          

HSP 2 Score: 128.257 bits (321), Expect = 1.098e-29
Identity = 104/281 (37.01%), Postives = 136/281 (48.40%), Query Frame = 3
Query: 2580 GKGYQKIGDNC-----IDINECDSLACH-NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G GY   GD        DINEC       +N +C NT GSF C C N    L+ E      I+EC     I  +           C ++ G   C C  GF   + + + C+DI+EC  NP L +  TC N+ GSF C CP G   +     C+D+DEC  S     NG C NMIGSY+C+C+ GY    D+ GC+DIDEC      C   +  C N+ GS++C C  G  L    + C DIDEC+ N    +G  C N  G + C C  GF+ + D K C
Sbjct:  951 GPGYSNRGDPVGRHFFKDINECKVFPQMCSNGKCRNTIGSFKCNCENGF-VLDAEGKNCTDIDECKISHDICGM---------GTCVNTPGSFDCECMEGFESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFKCLCPPGHDLNEDGTVCLDVDECSLSGNLCRNGRCVNMIGSYQCSCNTGYSATPDRQGCVDIDECTIMNGGC---DTHCSNSDGSYECSCERGYALLPDTRSCSDIDECEDNPYLCDGGQCTNVPGEYRCLCYDGFISSVDMKTC 1218          

HSP 3 Score: 121.324 bits (303), Expect = 1.514e-27
Identity = 108/296 (36.49%), Postives = 136/296 (45.95%), Query Frame = 3
Query: 2577 CGKGYQKI--GDNCIDINECD-SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTC------RSPANCSSSNGV-EFC-VCPRGFTYDLKEGCI----------DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY +   G+ CID NEC        N RCIN  GS+ C C+        E  C        F   I S+C  V+       +S   C    G  + C +CP   T   K+ C           DI+EC + P +     C NS GS+ C C  G+   +   GCVD+DEC  S K C  N IC+N  GSY+CAC  GY   ED + C D+DEC + +  CQ    +C NT G F C CP G       C+D +EC  Q  TC     C NS G F+C C KGF  +N    CE
Sbjct: 2222 CPPGYTRRPDGEGCIDENECRLKPGICENGRCINNVGSYKCVCNEGFTLNQDETECVDSRRGFCFTEVIQSLCQMVSSSRNLVTKSECCCDGGRGWGQQCDICPLPGTAQYKKLCPHGPGYTTDGRDIDECTVMPGMCHHGQCINSEGSYHCMCNNGYTTDITRTGCVDLDECAQSPKPC--NFICKNTEGSYQCACPRGYIMQEDGRTCRDLDECASKQHNCQ---FLCVNTIGGFTCKCPPGFTQHHTACIDNNECVSQPGTCGSKGVCQNSPGSFSCECQKGFSLDNSGSNCE 2512          

HSP 4 Score: 120.553 bits (301), Expect = 2.488e-27
Identity = 97/279 (34.77%), Postives = 135/279 (48.39%), Query Frame = 3
Query: 2574 NCGKGYQKIGD--NCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            +C  GY    D   C+DI+EC  +    ++ C N+ GS+ C C      L     C+  I+EC        +CD         C++  G   C+C  GF  + D+K  C D+NEC +NP  C+    C N+ GSF C C  GF       GC D+DEC   +  C  +  C N+ GS++C C  GY + +   C D+DEC      C  +   C N PG+F CVC  G     + CVD+DEC +N    E   C+NS GG+ C C  GFV   D + C+
Sbjct: 1121 SCNTGYSATPDRQGCVDIDECTIMNGGCDTHCSNSDGSYECSCERGYALLPDTRSCS-DIDEC---EDNPYLCDG------GQCTNVPGEYRCLCYDGFISSVDMKT-CSDVNECELNPNICI-HGKCENTRGSFICHCKVGFTVKKGSTGCTDVDECEIGAHNCDMHATCANVQGSFKCTCIEGY-FGDGVKCQDVDECANGTHNCIPE-AKCVNIPGTFTCVCRDGFSGDGQTCVDMDECAENVNLCEFGQCLNSAGGYRCECEMGFVPTVDGRSCK 1385          

HSP 5 Score: 113.62 bits (283), Expect = 3.123e-25
Identity = 103/297 (34.68%), Postives = 136/297 (45.79%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIE-------------------ECSFERKIISICDAVTCRSPANCSSSN-----------GVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            G GY   G    DI+EC  +   CH+  +CIN+ GS+ C C+N         GC    E                   +C+  R  I   D  TCR    C+S             G   C CP GFT      CID NEC   P  C  K  C+NS GSF C C KGF   +    C D+DEC  +  C     CQNM+G YRC C +GY ++ +   C+D +EC  + ++C   +  C NT GSFKC CP G  + +   GC D++EC  +   P    C N+ GG+ C C  G+
Sbjct: 2339 GPGYTTDGR---DIDECTVMPGMCHH-GQCINSEGSYHCMCNNGYTTDITRTGCVDLDECAQSPKPCNFICKNTEGSYQCACPRGYIMQEDGRTCRDLDECASKQHNCQFLCVNTIGGFTCKCPPGFTQH-HTACIDNNECVSQPGTCGSKGVCQNSPGSFSCECQKGFSLDNSGSNCEDMDECSGNHRCQHG--CQNMLGGYRCGCPSGYLQHFQWNQCVDENEC-TNPQVC--GSASCYNTLGSFKCACPNGYNFDQFSGGCNDVNECSHSKA-PCNFGCSNTEGGYLCGCPPGY 2624          

HSP 6 Score: 105.916 bits (263), Expect = 7.744e-23
Identity = 102/289 (35.29%), Postives = 132/289 (45.67%), Query Frame = 3
Query: 2568 DVNCGKGYQKI-GDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEEC--SFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYS-------VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE---YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            D  C KG+ +  G  C D+NEC+  A    NSRCINT GSF C C       +    C   ++E C  S+E +      AV  RS          + C C  G  +  + EGC +           K +   ++      CP+G  YS        HF   DI+EC+       NG C+N IGS++C C NG+    E K C DIDEC+ S  +C      C NTPGSF C C  G E      K C+DIDEC++N     G TC+N+ G F C C  G   N D   C
Sbjct:  834 DTICPKGFSRAKGTQCEDVNECEVFAGICVNSRCINTAGSFKCECPPGHTLESTGRTCIDLRLENCYLSYEEEECEEPLAVRLRS----------DVCCCSLGKAWGPECEGCPE-----------KGSSEYAF-----LCPRGPGYSNRGDPVGRHFF-KDINECKVFPQMCSNGKCRNTIGSFKCNCENGFVLDAEGKNCTDIDECKISHDIC--GMGTCVNTPGSFDCECMEGFESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFKCLCPPGHDLNEDGTVC 1093          

HSP 7 Score: 103.605 bits (257), Expect = 3.321e-22
Identity = 70/187 (37.43%), Postives = 97/187 (51.87%), Query Frame = 3
Query: 2811 ANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
              C +S G   C+C  G+T D+ + GC+D++EC  +P      C+N+ GS+ C CP+G+        C D+DEC  SK  +   +C N IG + C C  G+       CID +EC +    C G   VCQN+PGSF C C  G      G  C D+DEC  N+ C  G  C N  GG+ C C  G++
Sbjct: 2362 GQCINSEGSYHCMCNNGYTTDITRTGCVDLDECAQSPKPCNFICKNTEGSYQCACPRGYIMQEDGRTCRDLDECA-SKQHNCQFLCVNTIGGFTCKCPPGFTQ-HHTACIDNNECVSQPGTC-GSKGVCQNSPGSFSCECQKGFSLDNSGSNCEDMDECSGNHRCQHG--CQNMLGGYRCGCPSGYL 2543          

HSP 8 Score: 102.064 bits (253), Expect = 1.097e-21
Identity = 75/181 (41.44%), Postives = 98/181 (54.14%), Query Frame = 3
Query: 2853 PRGFTYDLKEG-CIDINECN-MNPCLDKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVF--NND 3371
            P G+  D+  G  IDI+EC+ +        C N  GSF C CP GF Y+ +   C DI+EC  + K+C  N  C N +GSYRC C  GY       CID DEC    ++C      CQ+T GS++C+CP G +  E    C+D+DEC++   C  G TC N+ G FNC C  GF    NND
Sbjct: 1692 PPGYEIDINTGERIDIDECSKIQGICTNGMCVNLPGSFRCECPMGFLYNDIQIICEDINECASAEKMCQRNAECINSVGSYRCECSPGYTQTPFGLCIDRDECSEKPEICSPGR--CQDTEGSYRCMCPNGYKATEDQNMCMDVDECERQ-PCGNG-TCRNTVGSFNCLCFPGFEISANND 1868          

HSP 9 Score: 95.9005 bits (237), Expect = 7.371e-20
Identity = 82/210 (39.05%), Postives = 102/210 (48.57%), Query Frame = 3
Query: 2652 NSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS--KICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVD 3263
            N RCINT GSF C C          F C     ECS    I + C   TC +        G E C C  GF       C D+NEC++NP L    C N++GS++C CP G+        C D DEC +      S N IC+N+IG+Y C C  GY    D +GCID +EC+    +C+  N  C N  GS+KCVC  G  L   E  CVD
Sbjct: 2083 NGRCINTDGSFRCDCPMGYNLEAAGFKCV-DANECS----IGNPCGNGTCLN-----VPGGFE-CTCDDGFEPGPMMTCEDLNECSLNPLLCAFRCVNNFGSYECMCPTGYVLREDRRMCKDQDECEEGLDNCASRNMICKNLIGTYMCICPPGYTRRPDGEGCIDENECRLKPGICE--NGRCINNVGSYKCVCNEGFTLNQDETECVD 2279          

HSP 10 Score: 88.1965 bits (217), Expect = 1.690e-17
Identity = 104/372 (27.96%), Postives = 145/372 (38.98%), Query Frame = 3
Query: 2511 RTTKELSQHLSKVFDITCKDVN------CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFG----------CAPKIEE--CSFERKIISICDAVTCRSPANCSSSNGVEFC-----------VCPRGFTY-----DLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNM----------------------------------------IGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            R   E  Q        TC+++       C  GY+   + C DI+EC S    C   S C+NT GSF C C +   ++  E G          C  +  +  CS  +   +      C         +  E C           +CP G        D +E   D++EC  NP +     C N+ GSF C CP G+   +  F CVD +EC     C GNG C N+                                         GSY C C  GY   ED+  C D DEC+     C   N++C+N  G++ C+CP G   +   +GC+D +EC+      E   CIN+ G + C CN+GF  N D   C
Sbjct: 1913 RDINECQQFRGMCAPGTCQNLEGSFRCMCPPGYEVKEEACTDIDECSSEEDLCMMGS-CLNTFGSFECRCPSG--FILSETGRKCYDVRVNYCFSEFAQGKCSTPKPFNTSKAGCCCSMMPGDGWGDPCEPCPKQNEEEAYRLLCPYGPGTVVGRDDSRE---DLDECLANPGICANGRCINTDGSFRCDCPMGYNLEAAGFKCVDANECSIGNPC-GNGTCLNVPGGFECTCDDGFEPGPMMTCEDLNECSLNPLLCAFRCVNNFGSYECMCPTGYVLREDRRMCKDQDECEEGLDNCASRNMICKNLIGTYMCICPPGYTRRPDGEGCIDENECRLKPGICENGRCINNVGSYKCVCNEGFTLNQDETEC 2277          

HSP 11 Score: 85.8853 bits (211), Expect = 9.089e-17
Identity = 75/231 (32.47%), Postives = 107/231 (46.32%), Query Frame = 3
Query: 2715 LNPEFGCA---PKIEECSFERKIISICDAVTCR-SPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            L P FG A   P+ +E   E  ++   + + C  SP    + N      C RGF  D + GC+  +  +   C+     R  + +F             F   DIDEC  + ++C  NG C N  GS++C C+ G+E   D + C+D DEC  +     G   +C N  GSFKC+C  G      G  C+DIDECQ +  C  G  CIN+ G F C C  G + + D + C
Sbjct:  385 LVPPFGPANFYPEGQEAQQELTLVLCANLILCVVSPGGIGALNQTH---CGRGFKSDFRGGCVAPD--SHQVCVFNINVRIHFAAF-----------TMFEFADIDECVYNGQVCK-NGRCVNTEGSFQCICNAGFELTADGRNCLDHDECSTTNMCLNG---MCINEDGSFKCICKPGFYLVPSGRYCLDIDECQTHGICMNGR-CINTEGSFKCECPPGMIVSMDGRLC 594          

HSP 12 Score: 81.6481 bits (200), Expect = 1.423e-15
Identity = 90/318 (28.30%), Postives = 132/318 (41.51%), Query Frame = 3
Query: 2574 NCGKGY--QKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINEC--NMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDE-----------CQ---ASKKLCQGDNVVCQNTPG--------------SFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQP 3416
            +C  GY  +  G  C+D NEC       N  C+N PG F C C +  E   P   C   + ECS    + +            C ++ G   C+CP G+   + +  C D +EC   ++ C  +   C+N  G++ C CP G+       GC+D +ECR       NG C N +GSY+C C+ G+   +D+  C+D              CQ   +S+ L       C    G               +K +CP G  Y   G  DIDEC           CINS G ++C CN G+  +     C +LD+     +P
Sbjct: 2096 DCPMGYNLEAAGFKCVDANECSIGNPCGNGTCLNVPGGFECTCDDGFE-PGPMMTCE-DLNECSLNPLLCAF----------RCVNNFGSYECMCPTGYVLREDRRMCKDQDECEEGLDNCASRNMICKNLIGTYMCICPPGYTRRPDGEGCIDENECRLKPGICENGRCINNVGSYKCVCNEGFTLNQDETECVDSRRGFCFTEVIQSLCQMVSSSRNLVTKSECCCDGGRGWGQQCDICPLPGTAQYKKLCPHGPGYTTDG-RDIDECTVMPGMCHHGQCINSEGSYHCMCNNGYTTDITRTGCVDLDECAQSPKP 2400          

HSP 13 Score: 79.337 bits (194), Expect = 8.975e-15
Identity = 89/319 (27.90%), Postives = 132/319 (41.38%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYV-EDKGCIDID------------------ECQA------SKKLC-----QGDNVVCQNTP----GSFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN 3404
            C +GY   G  C D++EC   +  C   ++C+N PG+F C C +     + +      ++EC+   + +++C+         C +S G   C C  GF   +    C DI+EC  +       C N  G F C C  G++     G C D DEC D   C  NGIC N  GS+ C C   Y+     +GC+  D                   C        S+  C     +     C+  P      ++ +CP G  ++         DIDEC +     +G  C N+FG F C C  GF FN D + CE +D+  N
Sbjct: 1290 CIEGYFGDGVKCQDVDECANGTHNCIPEAKCVNIPGTFTCVCRDG---FSGDGQTCVDMDECA---ENVNLCEF------GQCLNSAGGYRCECEMGFVPTVDGRSCKDIDECMFDNICVFGKCENVPGMFRCVCDPGYEIDETGGNCTDKDECADPSNCF-NGICTNTQGSFLCTCPADYQMSPSGQGCVGTDYRVGKCFLESTERGDGTLSCNVEIGIGISRSSCCCSLGRAWGAPCELCPVVNTTEYRILCPGGEGFRPNPYTVILEDIDECTELPGLCQGGNCQNTFGSFKCVCQPGFRFNEDNRQCEDIDECAN 1595          

HSP 14 Score: 77.7962 bits (190), Expect = 2.226e-14
Identity = 85/296 (28.72%), Postives = 128/296 (43.24%), Query Frame = 3
Query: 2616 DINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI---CSGNGICQNMIGSY-------RCACHN-GYEYVE----------------------DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            DI+EC  +   C N  RC+NT GSF C C+   E       C    +ECS     ++            C + +G   C+C  GF Y +  G  C+DI+EC  +       C N+ GSF C CP G   S+     D   C D+ +   C G+ +    +  +        C C N  + + E                      D G  DI+EC  + ++C   N  C+N  G+++CVC +G E    G  CVD+DEC       +  +C N+ G + C C  G++F ++T+ CE
Sbjct:  472 DIDECVYNGQVCKN-GRCVNTEGSFQCICNAGFELTADGRNCLDH-DECSTTNMCLN----------GMCINEDGSFKCICKPGF-YLVPSGRYCLDIDECQTHGICMNGRCINTEGSFKCECPPGMIVSM-----DGRLCIDTHMRSTCYGDYMMGKCLTPFPGANTKSECCCANPNFAFGEPCLPCPAVNSDDYKMLCPTPGFDNGGKDINECALNPEICT--NGQCENQLGTYRCVCNIGYESDITGKKCVDVDECMLRAHLCDNGSCRNTPGSYTCACPAGYLFKHETEMCE 747          

HSP 15 Score: 77.411 bits (189), Expect = 3.476e-14
Identity = 74/233 (31.76%), Postives = 95/233 (40.77%), Query Frame = 3
Query: 2484 PRS-IYQLSVRTTKELSQHLSK-----------VFDITCKDVNCGKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            PR  I Q   RT ++L +  SK           +   TCK   C  G+ +    CID NEC S    C +   C N+PGSF C C       N    C   ++ECS              R    C +  G   C CP G+    +   C+D NEC        A+C N+ GSF C CP G+ +     GC D++EC  SK    N  C N  G Y C C  GY       C+
Sbjct: 2417 PRGYIMQEDGRTCRDLDECASKQHNCQFLCVNTIGGFTCK---CPPGFTQHHTACIDNNECVSQPGTCGSKGVCQNSPGSFSCECQKGFSLDNSGSNCE-DMDECSGNH-----------RCQHGCQNMLGGYRCGCPSGYLQHFQWNQCVDENECTNPQVCGSASCYNTLGSFKCACPNGYNFDQFSGGCNDVNECSHSKA-PCNFGCSNTEGGYLCGCPPGYFRAGQGHCV 2633          

HSP 16 Score: 66.6254 bits (161), Expect = 6.617e-11
Identity = 89/342 (26.02%), Postives = 133/342 (38.89%), Query Frame = 3
Query: 2577 CGKGYQ--KIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP---KIEECSFERK-------------IISICDAVTCRSPAN--------CSSSNGVEF-CVCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDI------------------------DEC-------QASKKLC--------QGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+  + G NC D +EC   +   N  C NT GSF+C C  + +      GC     ++ +C  E                I I  +  C S           C   N  E+  +CP G  +      +   DI+EC   P L     C+N++GSF C C  GF+++  +  C DIDEC +     G G C N++G+Y C C   Y  V     C+D+                         EC       +A  + C        +  N +C   P  ++    + +   E+  +DIDEC +         C+N  G F C C  GF++N+    CE
Sbjct: 1412 CDPGYEIDETGGNCTDKDECADPSNCFNGICTNTQGSFLCTCPADYQMSPSGQGCVGTDYRVGKCFLESTERGDGTLSCNVEIGIGISRSSCCCSLGRAWGAPCELCPVVNTTEYRILCPGGEGFRPNPYTVILEDIDECTELPGLCQGGNCQNTFGSFKCVCQPGFRFNEDNRQCEDIDECANFPTVCGPGTCYNIVGNYTCVCPPDYMQVNGGNNCMDMRKSFCYKTYDAAGCDNELSFNLTEKECCCSYNVGKAWNRPCVPCPTISTKAHNQLCGGVPPGYE----IDINTGER--IDIDECSKIQGICTNGMCVNLPGSFRCECPMGFLYNDIQIICE 1747          

HSP 17 Score: 65.0846 bits (157), Expect = 1.846e-10
Identity = 86/302 (28.48%), Postives = 127/302 (42.05%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD----AVTCRSPANCSSSNGVEF----------CVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVD--IDEC-----------------RDSKICSG---------NGICQNMIGSYRCACHNGYEYVEDKGCI------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C D++EC+S  C N + C NT GSF C C    +    +  C    +   +   I + C+      T +S   C S+ G  +           +CP+GF+      C D+NEC +    C++ + C N+ GSF C CP G    S    C+D  ++ C                 R    C            G  +     Y   C  G  Y      +      DI+EC+   ++C   N  C+NT GSFKC C  G  L+ + K C DIDEC+ ++      TC+N+ G F+C C +GF
Sbjct:  746 CEDVDECESNPCINGN-CQNTVGSFTCVCLPTSKLDQTKTICIESTKGTCWLNIIDNRCEVNVNGATLKS--ECCSTLGAAWGSPCEPCELDTICPKGFSRAKGTQCEDVNECEVFAGICVN-SRCINTAGSFKCECPPGHTLESTGRTCIDLRLENCYLSYEEEECEEPLAVRLRSDVCCCSLGKAWGPECEGCPEKGSSEYAFLCPRGPGYSNRGDPVGRHFFKDINECKVFPQMCS--NGKCRNTIGSFKCNCENGFVLDAEGKNCTDIDECKISHDICGMGTCVNTPGSFDCECMEGF 1041          

HSP 18 Score: 63.1586 bits (152), Expect = 7.206e-10
Identity = 60/217 (27.65%), Postives = 92/217 (42.40%), Query Frame = 3
Query: 2811 ANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKG--FQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCAC------------------HNGYEYVEDKGC-IDIDECQASKKLCQ--GDNVVCQNTPGSFKCVCPVGL-EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
              C +  G   CVC  G+  D+  + C+D++EC +   L D  +CRN+ GS+ C CP G  F++     C D+DEC  +   +GN  CQN +GS+ C C                     +  + D  C ++++      + C   G        P     +CP G    K   C D++EC+        S CIN+ G F C C  G    +  + C
Sbjct:  678 GQCENQLGTYRCVCNIGYESDITGKKCVDVDECMLRAHLCDNGSCRNTPGSYTCACPAGYLFKHETEM-CEDVDECESNPCINGN--CQNTVGSFTCVCLPTSKLDQTKTICIESTKGTCWLNIIDNRCEVNVNGATLKSECCSTLGAAWGSPCEPCELDTICPKGFSRAKGTQCEDVNECEVFAGICVNSRCINTAGSFKCECPPGHTLESTGRTC 891          

HSP 19 Score: 58.9214 bits (141), Expect = 1.366e-8
Identity = 41/96 (42.71%), Postives = 56/96 (58.33%), Query Frame = 3
Query: 2850 CPRGFTYDLKEG-CIDINECNMNPCLDKA-TCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDK 3125
            C RGF  +++ G C D++EC   P + K  TC N+ GS++C CP G + S +   C DIDEC     IC+G G C+N IGSY C C  G+    D+
Sbjct:  176 CRRGFIPNIRTGACQDVDECQAIPGMCKGGTCVNTVGSYECKCPLGHKQSELSQQCEDIDECETIPGICNG-GECKNTIGSYFCNCPAGFSTPPDR 270          

HSP 20 Score: 56.6102 bits (135), Expect = 5.801e-8
Identity = 36/97 (37.11%), Postives = 49/97 (50.52%), Query Frame = 3
Query: 3114 VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKT 3398
            +    C D+DECQA   +C+G    C NT GS++C CP+G +  E  + C DIDEC+       G  C N+ G + C C  GF    D   C   +T
Sbjct:  184 IRTGACQDVDECQAIPGMCKGG--TCVNTVGSYECKCPLGHKQSELSQQCEDIDECETIPGICNGGECKNTIGSYFCNCPAGFSTPPDRSSCFDQRT 278          

HSP 21 Score: 53.9138 bits (128), Expect = 3.656e-7
Identity = 87/332 (26.20%), Postives = 124/332 (37.35%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINECDSLACH--NNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVC-PRGFTYDLKEGCIDIN-------------ECNMNPCLDKATCRNSYGS----------FDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG--CID--IDECQASK--------------------KLCQGDNVVCQNTP----GSFKCVCPVGLEYKEKG-------CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+    G  C+D++EC  L  H  +N  C NTPGS+ C C     + +    C   ++EC     I             NC ++ G   CVC P       K  CI+               E N+N    K+ C ++ G+           D  CPKGF  +    C D++EC        N  C N  GS++C C  G+  +E  G  CID  ++ C  S                      L +     C+  P      +  +CP G  Y  +G         DI+EC+          C N+ G F C C  GFV + + K C
Sbjct:  691 CNIGYESDITGKKCVDVDEC-MLRAHLCDNGSCRNTPGSYTCACPAGYLFKHETEMCE-DVDECESNPCIN-----------GNCQNTVGSFTCVCLPTSKLDQTKTICIESTKGTCWLNIIDNRCEVNVNGATLKSECCSTLGAAWGSPCEPCELDTICPKGFSRAKGTQCEDVNECEVFAGICVNSRCINTAGSFKCECPPGHT-LESTGRTCIDLRLENCYLSYEEEECEEPLAVRLRSDVCCCSLGKAWGPECEGCPEKGSSEYAFLCPRGPGYSNRGDPVGRHFFKDINECKVFPQMCSNGKCRNTIGSFKCNCENGFVLDAEGKNC 1008          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006555.1 (pep scaffold:Pmarinus_7.0:GL476370:200676:210822:-1 gene:ENSPMAG00000005922.1 transcript:ENSPMAT00000006555.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 129.028 bits (323), Expect = 3.339e-30
Identity = 95/268 (35.45%), Postives = 136/268 (50.75%), Query Frame = 3
Query: 2586 GYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFN 3365
            GY   G  C+D++EC   S  C  NS C NTPGS+ C C         + G     E C+   + +    +  C   A C ++ G   C C  G+  +    C+D++EC +  N C   +TC+N+ GSF C C  GFQ +    CVD++EC+   S  C     C N +GSY C C  GY       C+D+DEC      C   N  C+NTPGSF+C C  G +   + CVD++ECQ + +  C  G+ C+N+ G +NC C  G++ N
Sbjct:  107 GYVGNGTVCLDVDECLLPSNNCSANSTCTNTPGSYQCQC---------DAGFQKTGEMCTDVDECLKF-GSAACGQGAYCVNTVGSYNCPCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQKTGEK-CVDVNECQTSGSAACGPGAYCVNTVGSYNCPCLAGY-IGNGTVCLDVDECLLPSNNCSA-NSTCKNTPGSFQCQCDAGFQKTGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCLCLAGYIGN 360          

HSP 2 Score: 126.331 bits (316), Expect = 2.239e-29
Identity = 94/268 (35.07%), Postives = 134/268 (50.00%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN---PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C  G+QK G+ C D++EC    S AC   + C+NT GS+ C C     Y+     C   ++EC              C + + C ++ G   C C  GF     E C+D+NEC  +    C   A C N+ GS++C C  G+  +    C+D+DEC   S  CS N  C+N  GS++C C  G++   +K C+D++ECQ S     G    C NT GS+ C+C  G       C+D+DEC   +N C   STC N+ G F C C+ GF
Sbjct:  145 CDAGFQKTGEMCTDVDECLKFGSAACGQGAYCVNTVGSYNCPCL--AGYIGNGTVCL-DVDECLLPSN--------NCSANSTCKNTPGSFQCQCDAGFQ-KTGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCPCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQKTGEK-CVDVNECQTSGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGF 398          

HSP 3 Score: 125.561 bits (314), Expect = 3.579e-29
Identity = 107/339 (31.56%), Postives = 153/339 (45.13%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN---PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYV------EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF---VFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKS 3542
            C  G+QK G+ C+D+NEC    S AC   + C+NT GS+ C C     Y+     C   ++EC              C + + C ++ G   C C  GF     E C+D+NEC  +    C   A C N+ GS++C C  G+  +    C+D+DEC   S  CS N  C+N  GS++C C  G++ V        + C+D++EC  S     G    C NT GS+ C+C  G       C+D+DEC   +N C   STC N+ G F C C+ GF         + C L  +  +   K  LV          N   I+  ML      YKL    ++ S
Sbjct:  228 CDAGFQKTGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCPCL--AGYIGNGTVCL-DVDECLLPSN--------NCSANSTCKNTPGSFQCQCDAGFQ-KTGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQNVFPPPSETGEKCVDVNECHTSGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQSDFHGFGMRQCNLGSSYKLSIHKISLVM---------NCQSILGMML------YKLSKCCVLGS 538          

HSP 4 Score: 122.479 bits (306), Expect = 3.863e-28
Identity = 96/278 (34.53%), Postives = 137/278 (49.28%), Query Frame = 3
Query: 2577 CGKGYQKIGDN--CIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISI-----CDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFN 3365
            C  GY  + D+  C D++EC S    C   S C N  G++ C C N+  YLN EF           +R  I +       +  C   A C+++ G   C C  G+  +    C+D++EC +  N C   +TC N+ GS+ C C  GFQ +    C D+DEC    S  C     C N +GSY C C  GY       C+D+DEC      C   N  C+NTPGSF+C C  G +   + CVD++ECQ + +  C  G+ C+N+ G +NC C  G++ N
Sbjct:   15 CVAGYAAVSDSRTCQDVDECQSGIYNCTQYSTCHNLDGNYTCIC-NSGYYLN-EF---------PMQRTCIDVDECEKAGSALCGHGAYCANTLGSYACRCLVGYVGNGTV-CLDVDECLLPSNNCSANSTCTNTPGSYQCQCDAGFQKTGEM-CTDVDECLKFGSAACGQGAYCVNTVGSYNCPCLAGY-IGNGTVCLDVDECLLPSNNCSA-NSTCKNTPGSFQCQCDAGFQKTGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCPCLAGYIGN 277          

HSP 5 Score: 120.168 bits (300), Expect = 1.702e-27
Identity = 91/266 (34.21%), Postives = 132/266 (49.62%), Query Frame = 3
Query: 2592 QKIGDNCIDINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN---PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFN 3365
             + G+ C+D+NEC    S AC   + C+NT GS+ C C     Y+     C   ++EC              C + + C ++ G   C C  GF     E C+D+NEC+ +    C   A C N+ GS++C C  G+  +    C+D+DEC   S  CS N  C+N  GS++C C  G++   +K C+D++ECQ       G    C NT GS+ C+C  G       C+D+DEC   +N C   STC N+ G F C C+ GF  N
Sbjct:  702 SETGEKCVDVNECQTSGSAACGPGAYCVNTVGSYNCPCL--AGYIGNGTLCL-DVDECLLPSN--------NCSANSTCKNTPGSFQCQCDAGFQ-KTGEKCVDVNECHTSGSAACGPGAYCVNTVGSYNCPCLAGYIGNGTL-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQKTGEK-CVDVNECQTFGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQSN 953          

HSP 6 Score: 116.701 bits (291), Expect = 2.326e-26
Identity = 93/267 (34.83%), Postives = 127/267 (47.57%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ-NNTCPEGSTCINSFGGFNCTCNKGF 3356
            C  GY   G  C+D++EC   S  C  NS C NTPGSF C C    +    +      + EC           +  C   A C ++ G   C C  G+  +    C+D++EC +  N C   +TC+N+ GSF C C  GFQ +    CVD++EC+   S  C     C N +GSY C C  GY       C+D+DEC      C   N  C+NTPGSF+C C  G +     C DI+EC +    C  G+ C N+ G + C C  G+
Sbjct:  739 CLAGYIGNGTLCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQKTGEK---CVDVNECHTS-------GSAACGPGAYCVNTVGSYNCPCLAGYIGNGTL-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQKTGEK-CVDVNECQTFGSAACGPGAYCVNTVGSYNCLCLAGY-IGNGTVCLDVDECLLPSNNCSA-NSTCKNTPGSFQCQCDAGFQSNATVCEDINECGRVPAVCGLGTVCENTVGSYRCPCAPGY 991          

HSP 7 Score: 92.8189 bits (229), Expect = 4.831e-19
Identity = 106/367 (28.88%), Postives = 159/367 (43.32%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGI-----CQNMIGSY-----RC------ACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNY-MDIVKDMLKNIITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKET 3602
            C  GY   G  C+D++EC   S  C  NS C NTPGSF C C    + + P      +  E   +        +  C   A C ++ G   C+C  G+  +    C+D++EC +  N C   +TC+N+ GSF C C  GFQ   H FG    +     K+ S + I     CQ+++G       +C      A  N +    + G  C+D++ECQ       G    C NT GS+ C+C  G       C+D+DEC   +N C   STC N+ G F C C+ GF   +      L   ++VV    + +  +  +    NY  ++ K+  KN           +I  ++ L        K I+I ET
Sbjct:  353 CLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQNVFPP---PSETGEKCVDVNECHTSGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQSDFHGFGMRQCNLGSSYKL-SIHKISLVMNCQSILGMMLYKLSKCCVLGSSAYSNVFPPPSETGEKCVDVNECQTFGSAACGPGAYCVNTVGSYNCLCLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQIQSSIHKSILGMMVSVVYIYIEFILQLFLARDYPNYSYELGKN--KNTAHQKNPTCPLLIFHFYGL--------KFIWISET 704          

HSP 8 Score: 88.1965 bits (217), Expect = 1.311e-17
Identity = 56/148 (37.84%), Postives = 78/148 (52.70%), Query Frame = 3
Query: 2931 ATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGY---EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
             TC ++ GSF C C  G+   S    C D+DEC+     C+    C N+ G+Y C C++GY   E+   + CID+DEC+ +     G    C NT GS+ C C VG       C+D+DEC   +N C   STC N+ G + C C+ GF
Sbjct:    2 GTCTDTIGSFKCGCVAGYAAVSDSRTCQDVDECQSGIYNCTQYSTCHNLDGNYTCICNSGYYLNEFPMQRTCIDVDECEKAGSALCGHGAYCANTLGSYACRCLVGYVGNGTVCLDVDECLLPSNNCSANSTCTNTPGSYQCQCDAGF 149          

HSP 9 Score: 65.855 bits (159), Expect = 8.298e-11
Identity = 40/111 (36.04%), Postives = 59/111 (53.15%), Query Frame = 3
Query: 3054 GICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE----KGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFN 3365
            G C + IGS++C C  GY  V D + C D+DECQ+    C      C N  G++ C+C  G    E    + C+D+DEC++  +  C  G+ C N+ G + C C  G+V N
Sbjct:    2 GTCTDTIGSFKCGCVAGYAAVSDSRTCQDVDECQSGIYNCT-QYSTCHNLDGNYTCICNSGYYLNEFPMQRTCIDVDECEKAGSALCGHGAYCANTLGSYACRCLVGYVGN 111          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000017.1 (pep scaffold:Pmarinus_7.0:GL477746:39241:65692:1 gene:ENSPMAG00000000015.1 transcript:ENSPMAT00000000017.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.102 bits (318), Expect = 1.286e-29
Identity = 104/302 (34.44%), Postives = 144/302 (47.68%), Query Frame = 3
Query: 2586 GYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE---LDKTLNVVQPKADLVFGIDTSESMK 3461
            GY   G  C+D++EC   S  C  NS C NTPG + C C         + G     E C+   + +    AV C   A C +  G   C C  G+  +    C+D++EC +  N C   +TC+N+ GSF C C  GFQ +    CVD++EC+   S  C     C N +GSY C C  GY       C+D+DEC      C   N  C+NTPGSF+C C  G +     C DIDEC +  T C  G+ C N+ G + C C  G+  N +    E   L    NV   +A ++FG   +E+ +
Sbjct:  560 GYVGNGTVCLDVDECLLPSNNCSANSTCTNTPGFYQCQC---------DAGFQKTGEMCTDVDECLKFGSAV-CGQGAYCVNMVGSYNCPCLAGYIGNGTV-CLDVDECQLPSNNCSANSTCKNTPGSFQCQCDAGFQKTAEI-CVDVNECQTSGSAACGPGAYCVNTVGSYNCQCLAGY-IGNGTVCLDVDECLLPSNNCSA-NSTCKNTPGSFQCQCDAGFQSNATVCEDIDECGRVPTVCGLGTVCENTVGSYRCPCAPGYQRNTNHTILETSFLYTFTNVPYHRASVIFGRPMTEAFE 847          

HSP 2 Score: 118.627 bits (296), Expect = 6.248e-27
Identity = 94/290 (32.41%), Postives = 138/290 (47.59%), Query Frame = 3
Query: 2577 CGKGYQKIGDN--CIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLN--PEFGC---------------APKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFN 3365
            C  GY  + D+  C D++EC S    C   S C N  G++ C C++  E  +  P  G                 P+  E   +        +  C   A C+++ G   C C  G+  +    C+D++EC +  N C   +TC N+ G + C C  GFQ +    C D+DEC    S +C     C NM+GSY C C  GY       C+D+DECQ     C   N  C+NTPGSF+C C  G +   + CVD++ECQ + +  C  G+ C+N+ G +NC C  G++ N
Sbjct:  445 CVAGYAVVSDSRTCQDVDECQSGIYNCTQYSTCRNLEGNYTCICNSGYESAHSIPTQGVYSFEFKHSNLSPPPQPPESGETCIDVDECQKAGSALCGHGAYCANTLGSYACRCLVGYVGNGTV-CLDVDECLLPSNNCSANSTCTNTPGFYQCQCDAGFQKTGEM-CTDVDECLKFGSAVCGQGAYCVNMVGSYNCPCLAGY-IGNGTVCLDVDECQLPSNNCSA-NSTCKNTPGSFQCQCDAGFQKTAEICVDVNECQTSGSAACGPGAYCVNTVGSYNCQCLAGYIGN 730          

HSP 3 Score: 111.309 bits (277), Expect = 9.175e-25
Identity = 89/263 (33.84%), Postives = 127/263 (48.29%), Query Frame = 3
Query: 2601 GDNCIDINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFN 3365
            G+ CID++EC    S  C + + C NT GS+ C C     Y+     C   ++EC              C + + C+++ G   C C  GF     E C D++EC       C   A C N  GS++C C  G+  +    C+D+DEC+  S  CS N  C+N  GS++C C  G++   +  C+D++ECQ S     G    C NT GS+ C C  G       C+D+DEC   +N C   STC N+ G F C C+ GF  N
Sbjct:  523 GETCIDVDECQKAGSALCGHGAYCANTLGSYACRCL--VGYVGNGTVCL-DVDECLLPSN--------NCSANSTCTNTPGFYQCQCDAGFQ-KTGEMCTDVDECLKFGSAVCGQGAYCVNMVGSYNCPCLAGYIGNGTV-CLDVDECQLPSNNCSANSTCKNTPGSFQCQCDAGFQKTAEI-CVDVNECQTSGSAACGPGAYCVNTVGSYNCQCLAGYIGNGTVCLDVDECLLPSNNCSANSTCKNTPGSFQCQCDAGFQSN 771          

HSP 4 Score: 103.219 bits (256), Expect = 2.736e-22
Identity = 90/266 (33.83%), Postives = 128/266 (48.12%), Query Frame = 3
Query: 2619 INEC--DSLACHNNSRCINTPGSFVCYCS---NNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGI-CQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGS-TCINSFGGFNCTCNKGFVFNNDTKFC 3383
            I+EC  +S  C  N+ C+NTPG + C C+       +++ +       +ECS +           C S  N  C++S G   C+C  G+     E   DINEC    N C   A C N+ GS+ C C  G++ +  + C DIDEC     C+ NG  C N  GSY C C  G+       C DI+EC A+  LCQ D   C N  GS+KC C  G       C D+DEC+++  C   +  C N+ G + C C  G+ F++  + C
Sbjct:    1 IDECALNSSLCVKNAHCVNTPGHYDCSCNAGFRGNGFIDCQESTCLHEDECSVKNN--------PCLSVNNSFCNNSIGSYSCICTSGYNGTTCEV-TDINECISGQNNCPVNANCTNTIGSYTCACKLGYEGN-GWNCKDIDECMTGNHCNSNGSECMNTDGSYYCTCALGFT-GNGTYCADINECTANPYLCQ-DTEECMNGIGSYKCECLPGYIRNGTICTDVDECKKSQPCDANTMVCSNTIGSYTCLCKAGYQFDSTARIC 254          

HSP 5 Score: 94.7449 bits (234), Expect = 1.294e-19
Identity = 82/226 (36.28%), Postives = 104/226 (46.02%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNG-ICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASK-KLCQGDNVVCQNTPGSFKCVCPVG 3233
            C  GY        DINEC S    C  N+ C NT GS+ C C     Y    + C   I+EC       S        + + C +++G  +C C  GFT +    C DINEC  NP  C D   C N  GS+ C C  G+  +    C D+DEC+ S+ C  N  +C N IGSY C C  GY++    + C D DEC  S    C     +CQN  GSF C C  G
Sbjct:   77 CTSGYNGTTCEVTDINECISGQNNCPVNANCTNTIGSYTCACK--LGYEGNGWNCK-DIDECMTGNHCNS--------NGSECMNTDGSYYCTCALGFTGNGTY-CADINECTANPYLCQDTEECMNGIGSYKCECLPGYIRNGTI-CTDVDECKKSQPCDANTMVCSNTIGSYTCLCKAGYQFDSTARICTDTDECVVSSLNNCSRTTGLCQNFDGSFTCQCKAG 289          

HSP 6 Score: 94.3597 bits (233), Expect = 1.534e-19
Identity = 93/293 (31.74%), Postives = 129/293 (44.03%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSL--ACHNNSRCINTPGSFVCYCS------NNEEYLNPEFGCAPKIEECSFERKII----SICDAVTCRSPANCSSSNGVEFC-VCPR----GFTY---------DLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGF 3356
            C  G+Q     C DI+EC  +   C   + C NT GS+ C C+       N   L   F          + R  +     + +A  C +  +    N VE C VC       F+Y         +  E C D++EC       C   A C N+ GS++C C  G+  +    C+D+DEC   S  CS N  C N  GSY+C C  G++     G  C+D+DEC      C   N  C+N PGSF+C C  G +     C DIDEC +  T C  G+ C N+ G + C C  G+
Sbjct:  764 CDAGFQSNATVCEDIDECGRVPTVCGLGTVCENTVGSYRCPCAPGYQRNTNHTILETSF--LYTFTNVPYHRASVIFGRPMTEAFECCTLIHYVEYNKVERCNVCSNTAENAFSYSNLPPSHPPETGEMCTDVDECLKFGSAACGQGAYCVNTVGSYNCPCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCTNTPGSYQCQCDAGFQRYIGNGTVCLDVDECLLPSNNCSA-NSTCKNMPGSFQCQCDAGFQSNATVCEDIDECGRVPTVCGLGTVCENTVGSYRCPCAPGY 1052          

HSP 7 Score: 81.6481 bits (200), Expect = 1.254e-15
Identity = 58/155 (37.42%), Postives = 83/155 (53.55%), Query Frame = 3
Query: 2790 AVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            +  C   A C ++ G   C C  G+  +    C+D++EC +  N C   +TC N+ GS+ C C  GFQ  +  G  C+D+DEC   S  CS N  C+NM GS++C C  G++      C DIDEC     +C G   VC+NT GS++C C  G +
Sbjct:  902 SAACGQGAYCVNTVGSYNCPCLAGYIGNGTV-CLDVDECLLPSNNCSANSTCTNTPGSYQCQCDAGFQRYIGNGTVCLDVDECLLPSNNCSANSTCKNMPGSFQCQCDAGFQ-SNATVCEDIDECGRVPTVC-GLGTVCENTVGSYRCPCAPGYQ 1053          

HSP 8 Score: 81.2629 bits (199), Expect = 1.485e-15
Identity = 62/180 (34.44%), Postives = 90/180 (50.00%), Query Frame = 3
Query: 2595 KIGDNCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYE 3110
            + G+ C D++EC    S AC   + C+NT GS+ C C     Y+     C   ++EC              C + + C+++ G   C C  GF   +  G  C+D++EC +  N C   +TC+N  GSF C C  GFQ +    C DIDEC R   +C    +C+N +GSYRC C  GY+
Sbjct:  886 ETGEMCTDVDECLKFGSAACGQGAYCVNTVGSYNCPCL--AGYIGNGTVCL-DVDECLLPSN--------NCSANSTCTNTPGSYQCQCDAGFQRYIGNGTVCLDVDECLLPSNNCSANSTCKNMPGSFQCQCDAGFQSNATV-CEDIDECGRVPTVCGLGTVCENTVGSYRCPCAPGYQ 1053          

HSP 9 Score: 77.0258 bits (188), Expect = 3.525e-14
Identity = 52/126 (41.27%), Postives = 63/126 (50.00%), Query Frame = 3
Query: 3006 CVDIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE-YKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFN 3365
            C D+DEC    S  C     C N +GSY C C  GY       C+D+DEC      C   N  C NTPGS++C C  G + Y   G  C+D+DEC   +N C   STC N  G F C C+ GF  N
Sbjct:  891 CTDVDECLKFGSAACGQGAYCVNTVGSYNCPCLAGY-IGNGTVCLDVDECLLPSNNCSA-NSTCTNTPGSYQCQCDAGFQRYIGNGTVCLDVDECLLPSNNCSANSTCKNMPGSFQCQCDAGFQSN 1014          

HSP 10 Score: 75.8702 bits (185), Expect = 6.881e-14
Identity = 55/164 (33.54%), Postives = 74/164 (45.12%), Query Frame = 3
Query: 2952 GSFDCFCPKGFQY-SVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVED--------------------------KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            GSF C C  G+   S    C D+DEC+     C+    C+N+ G+Y C C++GYE                              + CID+DECQ +     G    C NT GS+ C C VG       C+D+DEC   +N C   STC N+ G + C C+ GF
Sbjct:  439 GSFKCGCVAGYAVVSDSRTCQDVDECQSGIYNCTQYSTCRNLEGNYTCICNSGYESAHSIPTQGVYSFEFKHSNLSPPPQPPESGETCIDVDECQKAGSALCGHGAYCANTLGSYACRCLVGYVGNGTVCLDVDECLLPSNNCSANSTCTNTPGFYQCQCDAGF 602          

HSP 11 Score: 75.485 bits (184), Expect = 9.099e-14
Identity = 91/324 (28.09%), Postives = 126/324 (38.89%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQ-----------------------YSVHFG---------CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE---------DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGF--VFNNDTKFCELDKTL 3401
            C  G+QK  + C+D+NEC    S AC   + C+NT GS+ C C     Y+     C   ++EC              C + + C ++ G   C C  GF  +    C DI+EC   P  C     C N+ GS+ C C  G+Q                        SV FG         C  I     +K+   N +C N          N + Y            + C D+DEC        G    C NT GS+ C C  G       C+D+DEC   +N C   STC N+ G + C C+ GF     N T   ++D+ L
Sbjct:  681 CDAGFQKTAEICVDVNECQTSGSAACGPGAYCVNTVGSYNCQCL--AGYIGNGTVCL-DVDECLLPSN--------NCSANSTCKNTPGSFQCQCDAGFQSNATV-CEDIDECGRVPTVCGLGTVCENTVGSYRCPCAPGYQRNTNHTILETSFLYTFTNVPYHRASVIFGRPMTEAFECCTLIHYVEYNKVERCN-VCSNTA-------ENAFSYSNLPPSHPPETGEMCTDVDECLKFGSAACGQGAYCVNTVGSYNCPCLAGYIGNGTVCLDVDECLLPSNNCSANSTCTNTPGSYQCQCDAGFQRYIGNGTVCLDVDECL 984          

HSP 12 Score: 68.9366 bits (167), Expect = 1.023e-11
Identity = 97/314 (30.89%), Postives = 135/314 (42.99%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLACHNN-SRCINTPGSFVCYCS----NNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMN-PC-LDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKI--CS-GNGICQNMIGSYRCACHNGYE----YVEDKGC---IDIDECQASKKLCQ---GDNVVCQNTP-------------------GSFKCVCPVGLEYKEKG----CVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C  GY+  G NC DI+EC     C++N S C+NT GS+ C C+     N  Y      CA  I EC+    +        C+    C +  G   C C  G+   ++ G  C D++EC  + PC  +   C N+ GS+ C C  G+Q+ S    C D DEC  S +  CS   G+CQN  GS+ C C  GY         K C   + + +C+ +  LC    G  ++C  TP                    +  C  PVG  Y        C D+DEC +  + C +   C N  GGF C+C   F   N T
Sbjct:  118 CKLGYEGNGWNCKDIDECMTGNHCNSNGSECMNTDGSYYCTCALGFTGNGTY------CA-DINECTANPYL--------CQDTEECMNGIGSYKCECLPGY---IRNGTICTDVDECKKSQPCDANTMVCSNTIGSYTCLCKAGYQFDSTARICTDTDECVVSSLNNCSRTTGLCQNFDGSFTCQCKAGYTGDGVTCTGKLCFHVVGVPQCRGAVILCNLAVGVVLLCDFTPIKLYKKKVFDVDECNPPENATQPCKGPVGDLYGRDDAHCVCPDVDECIRGLHNCTQ--NCTNIEGGFTCSCFARFSSVNGT 411          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005398.1 (pep scaffold:Pmarinus_7.0:GL477786:13802:46188:-1 gene:ENSPMAG00000004892.1 transcript:ENSPMAT00000005398.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 118.627 bits (296), Expect = 2.726e-27
Identity = 108/313 (34.50%), Postives = 143/313 (45.69%), Query Frame = 3
Query: 2577 CGKGY--QKIGDNCIDINECDSL-ACHNNSRCINTPGSFVCYC------------SNNEEYLN--------PEFGCA--PKIEECSF---------------ERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDC-FCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE-KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C +GY   + G  CID++EC+   AC  N RC+NT G + C C            +   EY+N        P   C   P    C F               ER+         CR  A    +N       P      L     DI+EC N   C++K  C N  G+F+C  CP GF+ +    C D++EC +S     +G C ++ GSYRC+C+ G+    + K C D+DEC  +++ CQ  N  C NTPGSFKC CP G +  +   C DIDEC   N C EG TCIN+ G F C C  GF     T  C
Sbjct:   69 CSQGYAASRGGTECIDVDECERADACGGNGRCVNTAGGYTCNCDRGYSLSTDKGETRRSEYVNECENTILCPGGRCVNLPGSHRCEFCDPGYQWRGQTGGVDERRRPKTKSKERCRKLAQREITNTSTT---PPLSPPHLNLIAADIDECQNPQACINK-RCVNIPGAFECVLCPTGFK-AAGTKCEDVNECLNSTAVCRDGRCIDLQGSYRCSCNPGFAPSGNGKRCDDVDEC--AERPCQ--NGECVNTPGSFKCSCPPGFQLSDGTNCRDIDECSTGNEC-EGGTCINTVGSFQCGCQPGFAALPGTDLC 371          

HSP 2 Score: 101.293 bits (251), Expect = 6.698e-22
Identity = 76/224 (33.93%), Postives = 111/224 (49.55%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY--SVHFGC-VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYV-EDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCP 3227
            V C  G++  G  C D+NEC +S A   + RCI+  GS+ C C       NP F  +   + C      +  C    C++   C ++ G   C CP GF       C DI+EC+     +  TC N+ GSF C C  GF         C  D+DEC ++  CS +  C N +GS++C C  GY+   E  GC+DI+EC+   ++C  +  +C N  G+F C+CP
Sbjct:  236 VLCPTGFKAAGTKCEDVNECLNSTAVCRDGRCIDLQGSYRCSC-------NPGFAPSGNGKRC----DDVDECAERPCQN-GECVNTPGSFKCSCPPGFQLSDGTNCRDIDECSTGNECEGGTCINTVGSFQCGCQPGFAALPGTDLCCSADVDECDNATACSEHQYCINSMGSFQCHCDQGYQTAKEGLGCVDINECETITEVCGSE--LCDNEEGTFLCICP 445          

HSP 3 Score: 77.411 bits (189), Expect = 1.709e-14
Identity = 70/249 (28.11%), Postives = 104/249 (41.77%), Query Frame = 3
Query: 2802 RSPA-----NCSSSNGVEFCVCPRGFTYDLKEG-CIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVD----IDECRDSKICSGNGICQNMIGSYRCA-CHNGYEYVEDKGCID-----------------------------------------IDECQASKKLCQGDNVVCQNTPGSFKCV-CPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            RSPA      C ++ G   CVC +G+        CID++EC   + C     C N+ G + C C +G+  S   G       ++EC ++ +C G G C N+ GS+RC  C  GY++    G +D                                         IDECQ  +      N  C N PG+F+CV CP G +     C D++EC  +        CI+  G + C+CN GF  + + K C+
Sbjct:   48 RSPAPCPGGRCENTRGGFRCVCSQGYAASRGGTECIDVDECERADACGGNGRCVNTAGGYTCNCDRGYSLSTDKGETRRSEYVNECENTILCPG-GRCVNLPGSHRCEFCDPGYQWRGQTGGVDERRRPKTKSKERCRKLAQREITNTSTTPPLSPPHLNLIAADIDECQNPQAC---INKRCVNIPGAFECVLCPTGFKAAGTKCEDVNECLNSTAVCRDGRCIDLQGSYRCSCNPGFAPSGNGKRCD 292          

HSP 4 Score: 76.2554 bits (186), Expect = 4.265e-14
Identity = 40/111 (36.04%), Postives = 61/111 (54.95%), Query Frame = 3
Query: 3048 GNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNND 3371
            G+G C ++   + C C+ GY+       CID+DEC  S   C G    C+NT G F+CVC  G      G  C+D+DEC++ + C     C+N+ GG+ C C++G+  + D
Sbjct:   12 GHGECVHLDRGHTCVCNGGYKLNPSLTHCIDVDECARSPAPCPGGR--CENTRGGFRCVCSQGYAASRGGTECIDVDECERADACGGNGRCVNTAGGYTCNCDRGYSLSTD 120          

HSP 5 Score: 72.0182 bits (175), Expect = 8.688e-13
Identity = 51/142 (35.92%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 2844 CVCPRGFTYDLK-EGCIDINECNMNPC-LDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCID----IDECQASKKLCQGDNVVCQNTPGSFKC-VCPVGLEYK 3245
            CVC  G+  +     CID++EC  +P       C N+ G F C C +G+  S     C+D+DEC  +  C GNG C N  G Y C C  GY    DKG       ++EC+ +  LC G   V  N PGS +C  C  G +++
Sbjct:   25 CVCNGGYKLNPSLTHCIDVDECARSPAPCPGGRCENTRGGFRCVCSQGYAASRGGTECIDVDECERADACGGNGRCVNTAGGYTCNCDRGYSLSTDKGETRRSEYVNECE-NTILCPGGRCV--NLPGSHRCEFCDPGYQWR 163          

HSP 6 Score: 67.3958 bits (163), Expect = 2.165e-11
Identity = 89/340 (26.18%), Postives = 129/340 (37.94%), Query Frame = 3
Query: 2574 NCGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEF-----------CVCPRGFTY----DLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDEC------------QASKKLCQ---GDNVVCQNTPGSF----KCVCPVGLEYK---EKGCVDIDECQQ-NNTCPEG----------------------------STCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +C  G+   G+   C D++EC    C N   C+NTPGSF C C        P F                 + D   CR    CS+ N  E            C C  GF      DL     D++EC N   C +   C NS GSF C C +G+Q +    GCVDI+EC       G+ +C N  G++ C C N Y+  + +    + +C             A ++ C     D  VC+N  G      +C C VG  +    E     ++  ++ N  CPEG                            S C+N+  G+ C C  G++++     C
Sbjct:  277 SCNPGFAPSGNGKRCDDVDECAERPCQN-GECVNTPGSFKCSCP-------PGF----------------QLSDGTNCRDIDECSTGNECEGGTCINTVGSFQCGCQPGFAALPGTDLCCS-ADVDECDNATACSEHQYCINSMGSFQCHCDQGYQTAKEGLGCVDINECETITEVCGSELCDNEEGTFLCICPNDYQEFDSQ----LGKCVPEPTVGSQSHVPAERRECYFNVNDENVCRNVLGRNVTRDECCCTVGAGWGTDCEPSPCPLEGTREFNELCPEGKGLVPSPNGRGYRDADECTLFSQELCRDSLCVNTQAGYKCYCRTGYLYDRSRLHC 587          

HSP 7 Score: 67.0106 bits (162), Expect = 2.968e-11
Identity = 91/324 (28.09%), Postives = 129/324 (39.81%), Query Frame = 3
Query: 2574 NCGKGYQ-KIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD-AVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQ-YSVHFG-CVDIDEC-------RDSKICSGN----GICQNMIGSY----RCACHNGYEYVED------------------------------KGCIDIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            +C  G+Q   G NC DI+EC +        CINT GSF C C        P F   P  + C      +  CD A  C     C +S G   C C +G+ T     GC+DINEC  +        C N  G+F C CP  +Q +    G CV             + + C  N     +C+N++G       C C  G  +  D                              +G  D DEC   S++LC+  + +C NT   +KC C  G  Y      C D+DEC+ + +C +G  C NS G ++C C      ++  + C
Sbjct:  317 SCPPGFQLSDGTNCRDIDECSTGNECEGGTCINTVGSFQCGC-------QPGFAALPGTDLCC--SADVDECDNATACSEHQYCINSMGSFQCHCDQGYQTAKEGLGCVDINECETITEVCGSELCDNEEGTFLCICPNDYQEFDSQLGKCVPEPTVGSQSHVPAERRECYFNVNDENVCRNVLGRNVTRDECCCTVGAGWGTDCEPSPCPLEGTREFNELCPEGKGLVPSPNGRGYRDADECTLFSQELCR--DSLCVNTQAGYKCYCRTGYLYDRSRLHCTDVDECENSQSCVDGQ-CYNSEGSYSCYCTAPLTLDHTGRRC 628          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008854.1 (pep scaffold:Pmarinus_7.0:GL476328:1008604:1022137:1 gene:ENSPMAG00000008009.1 transcript:ENSPMAT00000008854.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 114.39 bits (285), Expect = 6.347e-26
Identity = 91/256 (35.55%), Postives = 124/256 (48.44%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNND 3371
            C DINEC     + +  C NT GSF C C +    +N        I EC              C S  +CS+ +G   C C RG+  +    C +INEC  + C   A C +  GSF C C  G+  +    C+DI+EC +  S +C   G C+N+ GSYRC C +GY    +  C D++EC      C G N +CQNTPG + C+C  G       C DI+EC   N C   ++CIN+ GGF C C   +  N +
Sbjct:  128 CFDINECMVNKGNCSQNCQNTEGSFACTCWSGYT-MNTTTKTCVDINECLNNP----------CGSSGSCSNIDGSYICNCLRGYQLNSARICENINECLTSACGPNAICTDLIGSFTCQCQPGYSSNGTM-CLDINECANTASPMCP-IGTCENLPGSYRCHCPSGYTAANNT-CRDVNECLIIPSPC-GSNSICQNTPGFYTCLCSSGFTSVSGVCQDINECLA-NPCAAPASCINTAGGFQCNCPLDYQLNGN 367          

HSP 2 Score: 113.235 bits (282), Expect = 1.689e-25
Identity = 94/305 (30.82%), Postives = 145/305 (47.54%), Query Frame = 3
Query: 2478 DIPRSIYQLSVRTTKELSQHLS--KVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--CVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCI-DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNND 3371
            +I  ++ Q +V   K  S++         +     C  GY   G  C D++EC    C  N+ C+NT GS+ C C+    YLN        + ECS         +  +C   + C ++ G   C C  G+  D+K  C D++EC  +    +  C N+ GS+ C C  G+  +   G  C+DIDEC++  +  C+ N +C N  GS++CAC     Y +  G   DI  C      C  + +   + P S +CVCP G     K C D++EC    TCP  +TC++  GGF C C  G++F+N+
Sbjct:  367 NICEAVNQCTVPGQKLCSENAVCISTLGTSAYTCQCKAGYTGNGTVCTDVDECVKKPCPVNTVCLNTFGSYTCQCAAGYIYLNNT---CTDLNECSSV-------NLFSCPPNSKCVNAIGSYSCQCVPGYQ-DVKGACQDVDECLRDNGGCQHLCFNNVGSYRCGCRPGYLSAGLNGYMCIDIDECQNPTLNNCNQNAVCTNTRGSFKCACK---PYFKGDGITCDIGRCGYPINSCDPNAICDISNPQSPRCVCPSGWVGTGKKCYDVNECLNPCTCPASTTCMDLDGGFKCICPPGYIFSNN 657          

HSP 3 Score: 112.079 bits (279), Expect = 3.178e-25
Identity = 92/283 (32.51%), Postives = 129/283 (45.58%), Query Frame = 3
Query: 2523 ELSQHLSKVF-DITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDEC-QASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            + S H    F   TC    C  G+   G NC DINEC +   + +  C+N  G + C C                I EC   +   S           NC ++ G   C C  G+T +   + C+DINEC  NPC    +C N  GS+ C C +G+Q +    C +I+EC  S  C  N IC ++IGS+ C C  GY       C+DI+EC   +  +C      C+N PGS++C CP G       C D++EC    + C   S C N+ G + C C+ GF 
Sbjct:   59 QTSAHCVNTFGSFTCA---CNAGFTGNGYNCQDINECLTNNGNCSDICVNEQGGYHCACPVGYTLDPINHAACFDINECMVNKGNCS----------QNCQNTEGSFACTCWSGYTMNTTTKTCVDINECLNNPCGSSGSCSNIDGSYICNCLRGYQLNSARICENINECLTSA-CGPNAICTDLIGSFTCQCQPGYS-SNGTMCLDINECANTASPMCPIG--TCENLPGSYRCHCPSGYTAANNTCRDVNECLIIPSPCGSNSICQNTPGFYTCLCSSGFT 324          

HSP 4 Score: 105.145 bits (261), Expect = 5.566e-23
Identity = 91/261 (34.87%), Postives = 121/261 (46.36%), Query Frame = 3
Query: 2610 CIDINECD-SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL--KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C D+NEC     C  ++ C+NT GSF C C  N  +    + C   I EC       S            C +  G   C CP G+T D      C DINEC +N       C+N+ GSF C C  G+  +     CVDI+EC ++  C  +G C N+ GSY C C  GY+    + C +I+EC  S     G N +C +  GSF C C  G       C+DI+EC    +  CP G TC N  G + C C  G+   N+T
Sbjct:   46 CEDVNECLLPQQCQTSAHCVNTFGSFTCAC--NAGFTGNGYNCQ-DINECLTNNGNCS----------DICVNEQGGYHCACPVGYTLDPINHAACFDINECMVNKGNCSQNCQNTEGSFACTCWSGYTMNTTTKTCVDINECLNNP-CGSSGSCSNIDGSYICNCLRGYQLNSARICENINECLTSAC---GPNAICTDLIGSFTCQCQPGYSSNGTMCLDINECANTASPMCPIG-TCENLPGSYRCHCPSGYTAANNT 288          

HSP 5 Score: 69.707 bits (169), Expect = 4.942e-12
Identity = 62/201 (30.85%), Postives = 88/201 (43.78%), Query Frame = 3
Query: 2958 FDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEY---KEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKS 3542
            F C C  GF  +   G C D++EC   + C  +  C N  GS+ CAC+ G+       C DI+EC  +   C     +C N  G + C CPVG          C DI+EC  N   C +   C N+ G F CTC  G+  N  TK C ++++ LN     +     ID S             + N +  Y+L S  I ++
Sbjct:   29 FYCQCKPGFAKNPTTGECEDVNECLLPQQCQTSAHCVNTFGSFTCACNAGFT-GNGYNCQDINECLTNNGNCSD---ICVNEQGGYHCACPVGYTLDPINHAACFDINECMVNKGNCSQ--NCQNTEGSFACTCWSGYTMNTTTKTCVDINECLNNPCGSSGSCSNIDGS------------YICNCLRGYQLNSARICEN 211          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Match: EDO35109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SME0])

HSP 1 Score: 130.183 bits (326), Expect = 9.451e-33
Identity = 104/276 (37.68%), Postives = 135/276 (48.91%), Query Frame = 3
Query: 2577 CGKGYQKIGD-NCIDINECD----SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNPCL---DKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNN 3368
            C  GY+  G   C D++EC        C   + C+N  G + C C     Y          I+ECS    +          S A C ++ G   C CP G +Y+   G C DI+EC  +P +   D  TC N+ GSF+C C KGF  S     C D+DEC D   C G GIC N IGS+ C+C  GYE+    K C DIDEC + K+ C  DN  C N+ GSF+C C  G +     C DIDEC  ++  CP  S C N+ G + C C KG+   N
Sbjct:   31 CNIGYRGDGRIKCDDVDECHPPDGGSPCGEFADCVNVDGGYECSCWAG--YTGNSRASCKDIDECSGSNDVCK-------DSNARCINTPGSYKCECPSGMSYNHGTGTCEDIDECRSSPAVCTADHMTCSNTQGSFECNCKKGFSLSADKKSCRDVDECIDDP-CQG-GICINTIGSFNCSCIKGYEFDTGLKQCEDIDECHSRKRYCH-DNAKCTNSIGSFECKCREGFDGNGIFCADIDECFSEDFECPANSVCANTQGAYKCHCWKGYEMKN 294          

HSP 2 Score: 122.479 bits (306), Expect = 4.155e-30
Identity = 82/206 (39.81%), Postives = 113/206 (54.85%), Query Frame = 3
Query: 2796 TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECN----MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-IC-SGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
             C S A CS++ G  +C C  G+  D +  C D++EC+     +PC + A C N  G ++C C  G+  +    C DIDEC  S  +C   N  C N  GSY+C C +G  Y    G C DIDEC++S  +C  D++ C NT GSF+C C  G  L   +K C D+DEC  +    +G  CIN+ G FNC+C KG+ F+   K CE
Sbjct:   13 ACTSNAACSNTVGSYYCRCNIGYRGDGRIKCDDVDECHPPDGGSPCGEFADCVNVDGGYECSCWAGYTGNSRASCKDIDECSGSNDVCKDSNARCINTPGSYKCECPSGMSYNHGTGTCEDIDECRSSPAVCTADHMTCSNTQGSFECNCKKGFSLSADKKSCRDVDECIDDPC--QGGICINTIGSFNCSCIKGYEFDTGLKQCE 216          

HSP 3 Score: 105.531 bits (262), Expect = 2.436e-24
Identity = 86/226 (38.05%), Postives = 117/226 (51.77%), Query Frame = 3
Query: 2607 NCIDINEC---DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCV 3260
            +C DI+EC   + +   +N+RCINTPGS+ C C +   Y N   G    I+EC   R   ++C A        CS++ G   C C +GF+    K+ C D++EC  +PC     C N+ GSF+C C KG+++      C DIDEC   K  C  N  C N IGS+ C C  G++     G  C DIDEC +    C   N VC NT G++KC C  G E K   C+
Sbjct:   86 SCKDIDECSGSNDVCKDSNARCINTPGSYKCECPSGMSY-NHGTGTCEDIDEC---RSSPAVCTA----DHMTCSNTQGSFECNCKKGFSLSADKKSCRDVDECIDDPCQ-GGICINTIGSFNCSCIKGYEFDTGLKQCEDIDECHSRKRYCHDNAKCTNSIGSFECKCREGFD---GNGIFCADIDECFSEDFECPA-NSVCANTQGAYKCHCWKGYEMKNGECM 298          

HSP 4 Score: 102.834 bits (255), Expect = 1.563e-23
Identity = 67/174 (38.51%), Postives = 84/174 (48.28%), Query Frame = 3
Query: 2889 IDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR---DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
             D +EC    N C   A C N+ GS+ C C  G++      C D+DEC        C     C N+ G Y C+C  GY       C DIDEC  S  +C+  N  C NTPGS+KC CP G+ Y      C DIDEC+ +      +  TC N+ G F C C KGF  + D K C
Sbjct:    2 ADRDECQSLPNACTSNAACSNTVGSYYCRCNIGYRGDGRIKCDDVDECHPPDGGSPCGEFADCVNVDGGYECSCWAGYTGNSRASCKDIDECSGSNDVCKDSNARCINTPGSYKCECPSGMSYNHGTGTCEDIDECRSSPAVCTADHMTCSNTQGSFECNCKKGFSLSADKKSC 175          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Match: EDO27742 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8E5])

HSP 1 Score: 132.88 bits (333), Expect = 4.035e-32
Identity = 110/321 (34.27%), Postives = 152/321 (47.35%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEF----GCAPK-------IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK-----------EKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
             L V T    +  ++     TC+   C +GY  +   C D+NEC     +    NS C NT GSF C C   +    P+F    G AP         +EC+            TC + A+C +++G   C C RG+T  + + C DI+EC +    C   A C N+ GS+ C C +GF   V+  C DIDEC      C  +  C N  GSY C C  GY  V +K C D++EC  +  +C G+N +C+NT GSFKC C  G   K              C DIDEC+ N +TCP    C+N+ G + C C  G+   N+T
Sbjct:  190 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCTDVNECTETPYICSGENSICENTDGSFKCSC---KPGYTPKFSKAVGDAPANLTSCDDTDECAL--------GVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCKDIDECALGVATCPASADCVNNNGSYTCRCKRGFTL-VNNTCKDIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNT 493          

HSP 2 Score: 124.405 bits (311), Expect = 2.991e-29
Identity = 112/323 (34.67%), Postives = 147/323 (45.51%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEF----GCAPK-------IEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK-----------EKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
             L V T    +  ++     TC+   C +GY  +   C D+NEC     +    NS C NT GSF C C   +    P+F    G AP        I+EC      IS C       PAN  C +++G   C C  G+T  +   C DINEC +    C   A C N+ GS+ C C  GF   V+  C DIDEC      C  +  C N  GSY C C  GY  V +K C D++EC  +  +C G+N +C+NT GSFKC C  G   K              C D DEC     TCP  + C+N+ G + C C +G+   N T
Sbjct:   14 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCTDVNECTETPYVCSGENSICENTDGSFKCSC---KPGYTPKFSKAVGDAPANLTSCDDIDECKLN---ISTC-------PANRYCVNNDGSYTCKCKPGYTL-VNNTCEDINECALGVANCPASADCVNNNGSYTCRCKPGFTL-VNNTCKDIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDVNECTETPYICSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDTDECALGVATCPASADCVNNDGSYTCRCKRGYTLVNKT 317          

HSP 3 Score: 118.627 bits (296), Expect = 1.747e-27
Identity = 97/307 (31.60%), Postives = 143/307 (46.58%), Query Frame = 3
Query: 2523 ELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS--------LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-ICSG-NGICQNMIGSYRCACHNGY-----EYVEDK-----GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
             + ++    F  +CK     K  + +GD   ++  CD           C  ++ C+N  GS+ C C      +N        I+EC+            TC + A+C ++NG   C C RGFT  +   C DI+EC +    C   A C N+ GS+ C C +G+   V+  C D++EC ++  +CSG N IC+N  GS++C+C  GY     + V D       C DIDEC+ +   C   N  C N  GS+ C C  G       C DIDEC      CP  + C+N+ G + C C +GF   N+T
Sbjct:  242 SICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDTDECALGVATCPASADCVNNDGSYTCRCKRGYTLVNKT---CKDIDECAL--------GVATCPASADCVNNNGSYTCRCKRGFTL-VNNTCKDIDECALGVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECKLNISTCPA-NRYCVNNDGSYTCKCKPGYTLVNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKRGFTLVNNT 534          

HSP 4 Score: 92.4337 bits (228), Expect = 5.423e-19
Identity = 83/269 (30.86%), Postives = 118/269 (43.87%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL---DKATCRNSYGSFDCFCPKGF--QYSVHFG--------CVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGC 3257
             L V T    +  ++     TC+   C +GY  +   C DI+EC      C  ++ C+N  GS+ C C      +N        I+EC+            TC + A+C +++G   C C RG+T  + + C D+NEC   P +   + + C N+ GSF C C  G+  ++S   G        C DIDEC+ +   C  N  C N  GSY C C  GY  V +  C DIDEC      C   +  C N  GS+ C C  G       C
Sbjct:  284 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCKDIDECALGVATCPASADCVNNNGSYTCRCKRGFTLVNNT---CKDIDECAL--------GVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNT-CEDIDECALGVANCPA-SADCVNNDGSYTCRCKRGFTLVNNTC 535          

HSP 5 Score: 82.8037 bits (203), Expect = 5.718e-16
Identity = 53/134 (39.55%), Postives = 69/134 (51.49%), Query Frame = 3
Query: 3012 DIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK-----------EKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            DIDEC      C  +  C N  GSY C C  GY  V +K C D++EC  +  +C G+N +C+NT GSFKC C  G   K              C DIDEC+ N +TCP    C+N+ G + C C  G+   N+T
Sbjct:    9 DIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNT 141          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Match: EDO29764 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2Q1])

HSP 1 Score: 130.183 bits (326), Expect = 1.341e-30
Identity = 104/320 (32.50%), Postives = 151/320 (47.19%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL---DKATCRNSYGSFDCFCPKGF--QYSVHFG--------CVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK-----------EKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            +L++ T       ++     TCK   C  GY  + + C DI+EC      C  ++ C+N  GS+ C C      +N        I+EC+            TC + A+C +++G   C C RG+T  + + C D+NEC   P +   + + C N+ GSF C C  G+  ++S   G        C DIDEC      C  +  C N  GSY C C  GY  V +K C D++EC  +  +C G+N +C+NT GSFKC C  G   K              C DIDEC+ N +TCP    C+N+ G + C C  G+   N+T
Sbjct:  243 KLNISTCPANRYCVNNDGSYTCK---CKPGYTHVNNTCEDIDECAHGVANCPASADCVNNDGSYTCRCKRGYTLVNNT---CKDIDECAL--------GVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDVNECTETPYVCSGENSICENTDGSFKCYCKPGYTPKFSKAVNDALANLTSCDDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNT 546          

HSP 2 Score: 123.25 bits (308), Expect = 1.784e-28
Identity = 106/321 (33.02%), Postives = 153/321 (47.66%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEF----GCAPK-------IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-ICSG-NGICQNMIGSYRCACHNGY-----EYVEDK-----GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
             L V T    +  ++     TC+   C +GY  +   C D+NEC     +    NS C NT GSF C C   +    P+F    G AP        I+EC+             C + A+C +++G   C C  G+T  +   CIDI+EC +    C  +A C N+ GS+ C C +G+   V+  C D++EC ++  +CSG N IC+N  GS++C+C  GY     E V D       C DIDEC+ +   C   N  C N  GS+ C C  G  +    C DIDEC      CP  + C+N+ G + C C +G+   N+T
Sbjct:   14 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCTDVNECTETPYVCSGENSICENTDGSFKCSC---KPGYTPKFSKAVGDAPANLTSCDDIDECAH--------GVANCPASADCVNNDGSYTCRCKPGYTL-VNNTCIDIDECALGVATCPARADCVNNDGSYTCRCKRGYTL-VNKTCTDVNECTETPYVCSGKNSICENTNGSFKCSCKPGYTPKFSEAVGDAPANLTSCDDIDECKLNISTCPA-NRYCVNNDGSYTCKCKPGYTHVNNTCEDIDECAHGVANCPASADCVNNDGSYTCRCKRGYTLVNNT 317          

HSP 3 Score: 115.546 bits (288), Expect = 5.869e-26
Identity = 106/308 (34.42%), Postives = 137/308 (44.48%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCA-----------PKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVF 3362
             L V T    +  ++     TC+   C +GY  +   C D+NEC     +    NS C NT GSF CYC   +    P+F  A             I+EC      IS C       PAN  C +++G   C C  G+T  +   C DI+EC +    C   A C N+ GS+ C C +GF   V+  C DIDEC      C  +  C N  GSY C C  GY  V +K C DIDEC+ +   C   N  C N  GS+ C C  G       C DIDEC      CP  + C+N+ G + C C  GF  
Sbjct:  419 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCTDVNECTETPYVCSGENSICENTDGSFKCYC---KPGYTPKFSKAVNDALANLTSCDDIDECKLN---ISTC-------PANRYCVNNDGSYTCKCKPGYTL-VNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKRGFTL-VNNTCKDIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDIDECKLNISTCPA-NRYCVNNDGSYTCKCKPGYTLVNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKPGFTL 706          

HSP 4 Score: 109.383 bits (272), Expect = 4.004e-24
Identity = 108/361 (29.92%), Postives = 148/361 (41.00%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEF----GCAPK-------IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL---DKATCRNSYGSFDCFCPKG----FQYSVH------FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED----------------------------------------KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
             L V T    +  ++     TC+   C +GY  +   C D+NEC     +    NS C NT GSF C C   +    P+F    G AP        I+EC+            TC + A+C +++G   C C RG+T  + + C D+NEC   P +   + + C N+ GSF C+C  G    F  +V+        C DIDEC+ +   C  N  C N  GSY C C  GY  V +                                          C DIDEC      C   +  C N  GS+ C C  G     K C DIDEC+ N +TCP    C+N+ G + C C  G+   N+T
Sbjct:  325 ALGVATCPASADCVNNDGSYTCR---CKRGYTLVNKTCTDVNECTETPYVCSGENSICENTDGSFKCSC---KPGYTPKFSKAVGDAPANLTSCDDIDECAL--------GVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCTDVNECTETPYVCSGENSICENTDGSFKCYCKPGYTPKFSKAVNDALANLTSCDDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKRGFTLVNNTCKDIDECALGVATCPA-SADCVNNDGSYTCRCKRGYTLVNKTCTDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTLVNNT 669          

HSP 5 Score: 79.337 bits (194), Expect = 8.374e-15
Identity = 51/134 (38.06%), Postives = 66/134 (49.25%), Query Frame = 3
Query: 3012 DIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK-----------EKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            DIDEC      C  +  C N  GSY C C  GY  V +K C D++EC  +  +C G+N +C+NT GSFKC C  G   K              C DIDEC      CP  + C+N+ G + C C  G+   N+T
Sbjct:    9 DIDECALGVATCPASADCVNNDGSYTCRCKRGYTLV-NKTCTDVNECTETPYVCSGENSICENTDGSFKCSCKPGYTPKFSKAVGDAPANLTSCDDIDECAHGVANCPASADCVNNDGSYTCRCKPGYTLVNNT 141          

HSP 6 Score: 72.7886 bits (177), Expect = 8.850e-13
Identity = 66/214 (30.84%), Postives = 98/214 (45.79%), Query Frame = 3
Query: 2499 QLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDK 3125
            +L++ T       ++     TCK   C  GY  + + C DI+EC      C  ++ C+N  GS+ C C      +N        I+EC+            TC + A+C +++G   C C RG+T  + + C DI+EC +N   C     C N+ GS+ C C  G+   V+  C DIDEC      C  +  C N  GSY C C  G+  V +K
Sbjct:  513 KLNISTCPANRYCVNNDGSYTCK---CKPGYTLVNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKRGFTLVNNT---CKDIDECAL--------GVATCPASADCVNNDGSYTCRCKRGYTL-VNKTCTDIDECKLNISTCPANRYCVNNDGSYTCKCKPGYTL-VNNTCEDIDECALGVANCPASADCVNNDGSYTCRCKPGFTLVYNK 710          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Match: EDO49410 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGE4])

HSP 1 Score: 129.028 bits (323), Expect = 2.089e-30
Identity = 100/273 (36.63%), Postives = 136/273 (49.82%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSL---ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDT 3374
            C KG+   G  C+D++EC S+    C  N+ C N+ GS+VC C   + Y      C   I+EC+ +           C + A C++  G   C C +GF  + K  CI  NEC    N C   A C N+ GSF C C KGF       C+D+DEC+  ++ C  N +C N +GS+ C C  GYE      C D++EC+  K  C   N +C NT G+F C C  G     K C+D DEC+   N C   + CINS G ++C C  GF  N  T
Sbjct:  237 CRKGFVGDGKTCVDVDECASVLHNKCDPNALCTNSVGSYVCRCK--KGYTGDGITC-KDIDECTNKTD--------DCDANALCTNVLGSFVCRCLKGFEGNGKT-CIHDNECESGANDCSPDAVCTNTPGSFVCRCNKGFTGDGKI-CIDVDECKTGQVKCGENEVCANSLGSFTCQCAEGYERDSQGKCADVNECKTGKHDCS-VNALCTNTDGTFICRCLRGYIGDGKTCIDFDECKLPKNDCDVNAECINSIGSYSCICKPGFTGNGKT 495          

HSP 2 Score: 126.331 bits (316), Expect = 1.733e-29
Identity = 101/276 (36.59%), Postives = 139/276 (50.36%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C KG++  G  CI  NEC+S A  C  ++ C NTPGSFVC C  N+ +      C   ++EC   +        V C     C++S G   C C  G+  D +  C D+NEC    + C   A C N+ G+F C C +G+       C+D DEC+  K  C  N  C N IGSY C C  G+     K C D +EC  S  LC   N +C NTPGS+ C C  G +   K C D+DEC + ++ C   + C+N+ G + C C +GF  N+  K C+
Sbjct:  320 CLKGFEGNGKTCIHDNECESGANDCSPDAVCTNTPGSFVCRC--NKGFTGDGKICI-DVDECKTGQ--------VKCGENEVCANSLGSFTCQCAEGYERDSQGKCADVNECKTGKHDCSVNALCTNTDGTFICRCLRGYIGDGKT-CIDFDECKLPKNDCDVNAECINSIGSYSCICKPGFTG-NGKTCTDKNECVGSDLLCD-PNALCTNTPGSYLCRCKSGFQGDGKTCADVDECIKSDDICGNNTECLNNVGSYTCKCKEGFEDNDQDKHCK 581          

HSP 3 Score: 125.946 bits (315), Expect = 1.943e-29
Identity = 106/301 (35.22%), Postives = 148/301 (49.17%), Query Frame = 3
Query: 2475 VDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECN---MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGF 3356
            VDI     +++  +      + +  +  TCK      G+Q  G  C DI+EC +   +C  N+ C NT GS+VC C   + +      C+  I+EC      ++ CDA      A C++  G   C C +GF  D K  C+D++EC     N C   A C NS GS+ C C KG+       C DIDEC + +  C  N +C N++GS+ C C  G+E    K CI  +EC++    C  D  VC NTPGSF C C  G     K C+D+DEC+     C E   C NS G F C C +G+
Sbjct:  125 VDINECEVRIADCSQNATCTNTNGSYSCTCK-----AGFQGNGIVCRDIDECQAGKYSCDPNALCTNTEGSYVCRCL--KGFTGDGLVCS-DIDECKVTE--LNNCDA-----NALCTNIFGTFVCRCRKGFVGDGKT-CVDVDECASVLHNKCDPNALCTNSVGSYVCRCKKGYTGD-GITCKDIDECTNKTDDCDANALCTNVLGSFVCRCLKGFEG-NGKTCIHDNECESGANDCSPD-AVCTNTPGSFVCRCNKGFTGDGKICIDVDECKTGQVKCGENEVCANSLGSFTCQCAEGY 406          

HSP 4 Score: 116.701 bits (291), Expect = 1.771e-26
Identity = 100/268 (37.31%), Postives = 133/268 (49.63%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQ--NNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C  GY+  G+ C D +EC +    C  NS CINTPGS+ C C   E Y     G     +EC+     I+ CD       + C+++ G   C C +GF  D +   +DINEC +    C   ATC N+ GS+ C C  GFQ +    C DIDEC+  K  C  N +C N  GSY C C  G+   +   C DIDEC+ ++      N +C N  G+F C C  G     K CVD+DEC    +N C   + C NS G + C C KG+ 
Sbjct:   30 CKPGYKGNGEICTDADECTATPGICGENSICINTPGSYQCSCK--EGYQPSSDGNCDDYDECAGG---IADCDI-----NSVCTNTLGSYKCTCKQGFVGDGRFP-VDINECEVRIADCSQNATCTNTNGSYSCTCKAGFQGN-GIVCRDIDECQAGKYSCDPNALCTNTEGSYVCRCLKGFTG-DGLVCSDIDECKVTELNNCDANALCTNIFGTFVCRCRKGFVGDGKTCVDVDECASVLHNKCDPNALCTNSVGSYVCRCKKGYT 284          

HSP 5 Score: 114.775 bits (286), Expect = 8.381e-26
Identity = 98/269 (36.43%), Postives = 128/269 (47.58%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYE-YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C +GY++     C D+NEC +    C  N+ C NT G+F+C C     Y+     C    +EC   +          C   A C +S G   C+C  GFT + K  C D NEC  +   C   A C N+ GS+ C C  GFQ      C D+DEC +   IC  N  C N +GSY C C  G+E   +DK C DIDEC      C   N +C N PG+F C C  G     K C DI+EC +    C   + C NS G +NC C +G+ 
Sbjct:  402 CAEGYERDSQGKCADVNECKTGKHDCSVNALCTNTDGTFICRCL--RGYIGDGKTCI-DFDECKLPKN--------DCDVNAECINSIGSYSCICKPGFTGNGKT-CTDKNECVGSDLLCDPNALCTNTPGSYLCRCKSGFQGDGKT-CADVDECIKSDDICGNNTECLNNVGSYTCKCKEGFEDNDQDKHCKDIDECSDGSHDCH-VNAICTNVPGTFLCRCINGFLGDGKLCADINECFEGTANCDINAECTNSVGSYNCQCMEGYT 656          

HSP 6 Score: 108.612 bits (270), Expect = 6.927e-24
Identity = 101/279 (36.20%), Postives = 131/279 (46.95%), Query Frame = 3
Query: 2616 DINECDS---LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ--QNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQPKAD 3425
            D++EC +     C N + CINTPGSF C C    +  N E       +EC+    I        C   + C ++ G   C C  G+       C D +EC   +  C   + C N+ GS+ C C +GF     F  VDI+EC      CS N  C N  GSY C C  G++      C DIDECQA K  C   N +C NT GS+ C C  G       C DIDEC+  + N C   + C N FG F C C KGFV   D K C ++D+  +V+  K D
Sbjct:    1 DVDECSTPGVATCSNLATCINTPGSFKCECKPGYKG-NGEI--CTDADECTATPGI--------CGENSICINTPGSYQCSCKEGYQPSSDGNCDDYDECAGGIADCDINSVCTNTLGSYKCTCKQGFVGDGRFP-VDINECEVRIADCSQNATCTNTNGSYSCTCKAGFQG-NGIVCRDIDECQAGKYSCD-PNALCTNTEGSYVCRCLKGFTGDGLVCSDIDECKVTELNNCDANALCTNIFGTFVCRCRKGFV--GDGKTCVDVDECASVLHNKCD 263          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Match: EDO38787 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBY9])

HSP 1 Score: 122.479 bits (306), Expect = 4.645e-30
Identity = 99/279 (35.48%), Postives = 139/279 (49.82%), Query Frame = 3
Query: 2577 CGKGYQKIGD--NCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN-CSSSNGVEFCVCPRGFTYDL-KEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C +GY    D  +C DINEC  D+  C  +  C NT GS++C C      L P+      I+EC  ER  +          P N C++  G  +C C +G+   +    CID++EC  +P L     CRN+ GSF+C C KG+  +     CVD++EC+  + +C  N  C N++GSYRC C  G+   +   C D+DEC      C  +   C NTPGS +CVC  G       CVDI+EC  +    E   CIN  G F C C  G++   + K C+
Sbjct:   71 CNQGYGLTADRMDCEDINECLVDNGRC--SEMCNNTLGSYLCDCGAGFA-LQPDGRTCRDIDEC-MERPDVC--------GPGNTCNNIEGGYYCTCGKGYKQSVDSRSCIDVDECQEDPRLCTNGVCRNTPGSFECLCAKGYAIAKGTTACVDVNECQTGQAMCHENARCANLVGSYRCECKPGF-IGDGSSCTDVDECTTGIAQCS-EQGTCVNTPGSHRCVCHEGYTGDGITCVDINECLADPNLCEHGQCINKAGSFMCDCEIGYLPTPNGKGCQ 335          

HSP 2 Score: 117.087 bits (292), Expect = 3.202e-28
Identity = 94/259 (36.29%), Postives = 130/259 (50.19%), Query Frame = 3
Query: 2613 IDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFV 3359
            ID++EC      C N   C+NTPGS+ C CS      + +  C   I EC+  + +        CR+   C +  G   C+C +G+  T D  + C DINEC ++       C N+ GS+ C C  GF        C DIDEC +   +C     C N+ G Y C C  GY + V+ + CID+DECQ   +LC   N VC+NTPGSF+C+C  G  +      CVD++ECQ     C E + C N  G + C C  GF+
Sbjct:    1 IDVDECTMYPQLCRN-GICVNTPGSYRCECSAGLTLDSIQNACV-DINECNTWQGL--------CRN-GRCVNKQGTFECICNQGYGLTADRMD-CEDINECLVDNGRCSEMCNNTLGSYLCDCGAGFALQPDGRTCRDIDECMERPDVCGPGNTCNNIEGGYYCTCGKGYKQSVDSRSCIDVDECQEDPRLCT--NGVCRNTPGSFECLCAKGYAIAKGTTACVDVNECQTGQAMCHENARCANLVGSYRCECKPGFI 245          

HSP 3 Score: 92.4337 bits (228), Expect = 7.289e-20
Identity = 66/171 (38.60%), Postives = 92/171 (53.80%), Query Frame = 3
Query: 2889 IDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDEC-QQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            ID++EC M P L +   C N+ GS+ C C  G    S+   CVDI+EC   +    NG C N  G++ C C+ GY    D+  C DI+EC      C   + +C NT GS+ C C  G   +  G  C DIDEC ++ + C  G+TC N  GG+ CTC KG+  + D++ C
Sbjct:    1 IDVDECTMYPQLCRNGICVNTPGSYRCECSAGLTLDSIQNACVDINECNTWQGLCRNGRCVNKQGTFECICNQGYGLTADRMDCEDINECLVDNGRC---SEMCNNTLGSYLCDCGAGFALQPDGRTCRDIDECMERPDVCGPGNTCNNIEGGYYCTCGKGYKQSVDSRSC 168          

HSP 4 Score: 61.2326 bits (147), Expect = 1.401e-9
Identity = 35/87 (40.23%), Postives = 46/87 (52.87%), Query Frame = 3
Query: 3132 IDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            ID+DEC    +LC+  N +C NTPGS++C C  GL     +  CVDI+EC           C+N  G F C CN+G+    D   CE
Sbjct:    1 IDVDECTMYPQLCR--NGICVNTPGSYRCECSAGLTLDSIQNACVDINECNTWQGLCRNGRCVNKQGTFECICNQGYGLTADRMDCE 85          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Match: FBN1 (fibrillin 1 [Source:NCBI gene;Acc:101171649])

HSP 1 Score: 144.05 bits (362), Expect = 8.997e-34
Identity = 98/268 (36.57%), Postives = 139/268 (51.87%), Query Frame = 3
Query: 2610 CIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLN 3404
            C DI+EC +   C  N+ C+N PGSF C C +   +  P   C  +  EC+    I +         P  C    G   CVCP G+ T   +  C+D++EC   PC    TC+N+ GS++C C  GFQ S +  C+D+DEC   +    NG C+N++GS+ C C++GYE   D + C DI+EC  +   C      C N  GS++C+CP G    E+ C DIDEC Q+       TC N+ G F C C +GF  +   + C LD  +N
Sbjct: 1817 CEDIDECQNGPVCQQNANCLNMPGSFRCECEHGFRFT-PTGQCLDR-NECNENPGICN---------PGQCIDILGSYRCVCPNGYKTTRDQSMCVDVDECERQPC-GNGTCKNTLGSYNCLCYPGFQNSHNGDCIDVDECSTQRGLCRNGNCRNIVGSFLCVCNDGYELTLDGRVCTDINECAVNPGTCGAG--TCVNMDGSYRCICPPGYYLHEETCEDIDECSQSPEICTFGTCSNTEGSFTCQCPEGFQLSASGRRC-LDIRVN 2069          

HSP 2 Score: 123.25 bits (308), Expect = 1.719e-27
Identity = 97/264 (36.74%), Postives = 131/264 (49.62%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKAT-CRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC  +    +N RC NT GSF C C N       E  C   I+EC     +              C ++ G   C C  GF   + + + C+DI+EC  NP L +   C N+ GSF C CP G + +     C DI+EC  S     NG C NM+GSY C C+ GY+  ED   C+DIDEC      C+     C N+ GS++C C  G  L   ++ C+DIDEC++N +  +G  C N  G + C C  GF+ + D K C
Sbjct: 1038 DINECRMINTLCSNGRCRNTIGSFRCRCDNGFALDADERNCT-DIDECRISPDLCGQ---------GTCVNTPGDFECNCFEGFESGFMMMKNCMDIDECERNPQLCRGGECVNTEGSFQCICPPGHEITPDGSACQDINECDLSDRLCRNGQCVNMVGSYYCNCNTGYKSTEDLVNCVDIDECTIENGGCE---TFCTNSEGSYECSCHNGYALMPDQRSCIDIDECEENPSTCDGGQCTNIPGTYQCLCFDGFMSSEDMKSC 1288          

HSP 3 Score: 115.546 bits (288), Expect = 3.957e-25
Identity = 94/258 (36.43%), Postives = 136/258 (52.71%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK-IEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C+DI+EC       N  C NT GS++C C     Y+  E G + + ++ECS ++                C ++ G   C CP GFT      CID NEC+ +P  C     C+N+ GSF+C C +GF   S    C D+DEC  +  C     CQN++G YRC+C  GY ++ +   C+D +EC  + ++C G    C NT GSF+C+CP G +Y++   GC DI+EC    N C  G  C N+ GG+ C C  G++
Sbjct: 2453 CVDIDECIQAPKPCNFICKNTEGSYLCSCPRG--YILQEDGKSCRDLDECSTKQHNCQF----------LCINTIGGFTCKCPPGFTQH-HSACIDNNECSSDPNLCGSNGVCQNTPGSFNCECQRGFSLDSNGQTCEDMDECDGNHRCQHG--CQNLVGGYRCSCPQGYLQHYQWNQCVDENEC-LNTQICGG--ASCHNTLGSFRCLCPSGFDYEQTSGGCADINECSTTQNPCKFG--CSNTDGGYLCGCPPGYL 2690          

HSP 4 Score: 111.694 bits (278), Expect = 6.000e-24
Identity = 88/276 (31.88%), Postives = 127/276 (46.01%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN--CSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G GY   G    DI+EC  +     N +C+NT GS+ C C              P         + + I + +    P N  C ++ G   C CPRG+   +  + C D++EC+      +  C N+ G F C CP GF    H  C+D +EC  D  +C  NG+CQN  GS+ C C  G+    + + C D+DEC  + +   G    CQN  G ++C CP G    Y+   CVD +EC     C  G++C N+ G F C C  GF +   +  C
Sbjct: 2404 GPGYTTDGR---DIDECRVMGNLCKNGQCVNTKGSYKCIC-------------IPGYTTDITSTQCVDIDECIQAPKPCNFICKNTEGSYLCSCPRGYILQEDGKSCRDLDECSTKQHNCQFLCINTIGGFTCKCPPGFTQH-HSACIDNNECSSDPNLCGSNGVCQNTPGSFNCECQRGFSLDSNGQTCEDMDECDGNHRCQHG----CQNLVGGYRCSCPQGYLQHYQWNQCVDENECLNTQIC-GGASCHNTLGSFRCLCPSGFDYEQTSGGC 2657          

HSP 5 Score: 111.309 bits (277), Expect = 6.865e-24
Identity = 101/300 (33.67%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDA------VTCRSPANCSSSNG----VEFC----------VCP--RGFTYDLKEGCIDINECN-MNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY +   GD CID+NEC +      N RC NT GS+ C C         +  C    E+  +   + ++C        +  +S   C    G     E C          +CP   G+T D +    DI+EC  M        C N+ GS+ C C  G+   +    CVDIDEC +  K C  N IC+N  GSY C+C  GY   ED K C D+DEC   +  CQ    +C NT G F C CP G       C+D +EC  + N C     C N+ G FNC C +GF  +++ + CE
Sbjct: 2287 CSPGYTRHPSGDGCIDLNECATKPGICRNGRCENTVGSYRCKCDQGFVANPTQTECIDNREDFCYTEVLTTLCQMSSSNRNLVTKSECCCDGGRGWGINCELCPLPGTSQYKKMCPLGPGYTTDGR----DIDECRVMGNLCKNGQCVNTKGSYKCICIPGYTTDITSTQCVDIDECIQAPKPC--NFICKNTEGSYLCSCPRGYILQEDGKSCRDLDECSTKQHNCQ---FLCINTIGGFTCKCPPGFTQHHSACIDNNECSSDPNLCGSNGVCQNTPGSFNCECQRGFSLDSNGQTCE 2577          

HSP 6 Score: 110.538 bits (275), Expect = 1.527e-23
Identity = 109/349 (31.23%), Postives = 156/349 (44.70%), Query Frame = 3
Query: 2601 GDNCIDINECD-SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY-DLKEGCIDINECNMNPC-LDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKIC-SGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNN-----------DTKFCELDKTLNVVQP-KADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAII------KSYFALTTGGN 3569
            G  C DINECD S     N +C+N  GS+ C C+   +       C   I+EC+ E      C+         C++S G   C C  G+     +  CIDI+EC  NP   D   C N  G++ C C  GF  S     C+D+DEC  +S IC SGN  C+N  GS+ C C  GY   +   GC DI+EC+     C      C NT GSFKC C  G       C D+DEC    ++C   + C N+ G + C C +G+  +            ++  CE    LN+    + +   G  T+   K   DI + +  +I  + + ++L  +        Y   T+GGN
Sbjct: 1160 GSACQDINECDLSDRLCRNGQCVNMVGSYYCNCNTGYKSTEDLVNCV-DIDECTIEN---GGCETF-------CTNSEGSYECSCHNGYALMPDQRSCIDIDECEENPSTCDGGQCTNIPGTYQCLCFDGFMSSEDMKSCLDVDECELNSNICLSGN--CENTKGSFICHCDLGYSVRKGTTGCTDINECEIGAHNCD-RQATCTNTAGSFKCNCAPGWIGNGLKCTDLDECSNGTHSCNNNAECQNTMGSYRCVCKEGYFGDGFFCSDSDECAENSDLCENGHCLNLPGGFRCECDMGFITTADGKACEDIDECIFADICVNGRCQNLPGLFRCECNIGYERDTSGGN 1494          

HSP 7 Score: 109.768 bits (273), Expect = 2.617e-23
Identity = 99/269 (36.80%), Postives = 133/269 (49.44%), Query Frame = 3
Query: 2607 NCIDINECD-SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRG--FTYDLKEGCIDINECNMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC DI+EC  S        C+NTPG F C C    E           I+EC    ++        CR    C ++ G   C+CP G   T D    C DINEC+++  L +   C N  GS+ C C  G++ +     CVDIDEC  ++  C     C N  GSY C+CHNGY  + D + CIDIDEC+ +   C G    C N PG+++C+C  G    E  K C+D+DEC+ N N C  G+ C N+ G F C C+ G+     T  C
Sbjct: 1077 NCTDIDECRISPDLCGQGTCVNTPGDFECNCFEGFESGFMMMKNCMDIDECERNPQL--------CRG-GECVNTEGSFQCICPPGHEITPD-GSACQDINECDLSDRLCRNGQCVNMVGSYYCNCNTGYKSTEDLVNCVDIDECTIENGGCET--FCTNSEGSYECSCHNGYALMPDQRSCIDIDECEENPSTCDGGQ--CTNIPGTYQCLCFDGFMSSEDMKSCLDVDECELNSNICLSGN-CENTKGSFICHCDLGYSVRKGTTGC 1330          

HSP 8 Score: 108.997 bits (271), Expect = 3.485e-23
Identity = 78/189 (41.27%), Postives = 100/189 (52.91%), Query Frame = 3
Query: 2832 GVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            G   C C  GF  D +  CID +EC  NPC     C N+ GS+ C CP GFQ +     C D+DEC  + +IC+ NG C N  GS+ C C+ G+E   D K C D DEC   + +C   N +C N  GSFKC+C  G  L+   + CVDIDEC+    C  G  C+N+ G F C C  G     D + C
Sbjct:  479 GTYRCECNMGFKLDRRGECIDDDECERNPCA-HGECVNTPGSYICQCPAGFQSTATRTECRDLDECVANGRICN-NGRCVNTEGSFHCVCNAGFEITADGKNCQDQDECLI-RNMCL--NGLCINEDGSFKCICKSGFLLDTSGRMCVDIDECETPGMCMNGH-CVNTEGSFRCECMAGMAVGLDGRVC 661          

HSP 9 Score: 104.375 bits (259), Expect = 1.001e-21
Identity = 97/290 (33.45%), Postives = 128/290 (44.14%), Query Frame = 3
Query: 2565 KDVNCGKGYQKI-GDNCIDINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG--------CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLC-QGDNVVCQNTPGSFKCVCPVGLE---YKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            KD  CGKG+ ++ G+ C D++EC        N +C+NT GSF C C            C   + E C          D   C +P      + V+ C C  G  +          EC+  P        N    F   CP+G  +S H G          DI+ECR       NG C+N IGS+RC C NG+    +++ C DIDEC+ S  LC QG    C NTPG F+C C  G E      K C+DIDEC++N     G  C+N+ G F C C  G     D   C+
Sbjct:  902 KDPICGKGFARVKGNVCEDVDECQVFPGVCINGKCVNTQGSFFCQCPPGMTVDVSGRTCIDLRTEHCYLTH------DDERCGTPI--PGRHRVDACCCSVGVAWGP--------ECDECP-------ENGTPEFAQLCPRGPGFS-HRGDFINGRPFLKDINECRMINTLCSNGRCRNTIGSFRCRCDNGFALDADERNCTDIDECRISPDLCGQG---TCVNTPGDFECNCFEGFESGFMMMKNCMDIDECERNPQLCRGGECVNTEGSFQCICPPGHEITPDGSACQ 1164          

HSP 10 Score: 92.8189 bits (229), Expect = 3.016e-18
Identity = 83/223 (37.22%), Postives = 109/223 (48.88%), Query Frame = 3
Query: 2583 KGYQK-IGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDECRDS-KICSGNGI-CQNMIGSYRCACHNGY-EYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL 3236
            +GY++   DN  DINEC +  C N  +CINT GSF C C            C   I EC     I + C   TC +     +  G E C C  GF       C DINEC+ NP L    C N  GS++C CP G+        C D +EC D    C+  G+ C+N+IG+Y C C  GY  +    GCID++EC     +C+  N  C+NT GS++C C  G 
Sbjct: 2125 EGYERGKDDNPTDINECLNNPCVN-GQCINTDGSFRCECPIGYNLDTSGVTCI-DINEC----DIGNPCGNGTCTN-----AIGGFE-CTCDEGFEPGPMMSCEDINECSQNPLLCAFRCVNVVGSYECKCPTGYVLREDRRMCKDQNECEDGIDDCASRGMTCKNLIGTYMCICSPGYTRHPSGDGCIDLNECATKPGICR--NGRCENTVGSYRCKCDQGF 2333          

HSP 11 Score: 92.0485 bits (227), Expect = 5.389e-18
Identity = 99/314 (31.53%), Postives = 133/314 (42.36%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDK-GCIDIDECQASKK-LCQGD---NVVCQNTPG-----------------------------SFKCVCPVGLEYKEKGCV----DIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            NC  G+   G  C D++EC   + +C+NN+ C NT GS+ C C   E Y    F C+   +EC+    +             +C +  G   C C  GF  T D K  C DI+EC          C+N  G F C C  G++     G C DI+EC D   C  NGIC N+ G+Y C C   +E      GC+DI         L +GD   N VC    G                              +K +CP G  ++         DI+ECQ+     +G  CIN+FG F C C +GF  N DT+ CE
Sbjct: 1359 NCAPGWIGNGLKCTDLDECSNGTHSCNNNAECQNTMGSYRCVCK--EGYFGDGFFCS-DSDECAENSDLCEN---------GHCLNLPGGFRCECDMGFITTADGK-ACEDIDECIFADICVNGRCQNLPGLFRCECNIGYERDTSGGNCTDINECADPTTCI-NGICVNIPGTYLCNCPPDFELNPTGVGCVDIRSGSCYLNVLARGDASENYVCSQEIGVGVSKASCCCSEGAGWGSPCEACPHVNSTGYKTLCPGGEGFRPNPITVILEDINECQELPGLCQGGQCINTFGSFQCECPRGFSLNLDTRVCE 1658          

HSP 12 Score: 88.1965 bits (217), Expect = 7.500e-17
Identity = 93/289 (32.18%), Postives = 124/289 (42.91%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP-KIEEC--SFER------KIISICDAVTC--RSPANCSSSNGVEFC----------VCP-RGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQ--NNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C  GY    + C DI+EC  S        C NT GSF C C    +       C   ++  C   FE       K  +I     C  R P      +  E C          +CP  G+     +   DINEC  NPC++   C N+ GSF C CP G+        C+DI+EC     C GNG C N IG + C C  G+E      C DI+EC  +  LC      C N  GS++C CP G   +E  + C D +EC+   ++    G TC N  G + C C+ G+ 
Sbjct: 2010 CPPGYYLHEETCEDIDECSQSPEICTFGTCSNTEGSFTCQCPEGFQLSASGRRCLDIRVNYCFTKFENGRCLVPKPQNISKEACCCTRMPGQ-GWGDPCELCPAKDQEAYRVLCPNEGYERGKDDNPTDINECLNNPCVN-GQCINTDGSFRCECPIGYNLDTSGVTCIDINECDIGNPC-GNGTCTNAIGGFECTCDEGFEPGPMMSCEDINECSQNPLLCA---FRCVNVVGSYECKCPTGYVLREDRRMCKDQNECEDGIDDCASRGMTCKNLIGTYMCICSPGYT 2292          

HSP 13 Score: 88.1965 bits (217), Expect = 8.367e-17
Identity = 87/236 (36.86%), Postives = 111/236 (47.03%), Query Frame = 3
Query: 2580 GKGYQKIGDNCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK--GCVDI 3266
            G   +K    C DINEC+  A  C   + C NT GSF C C+    ++     C   ++ECS            +C + A C ++ G   CVC  G+  D    C D +EC  N  L +   C N  G F C C  GF  +     C DIDEC  + IC  NG CQN+ G +RC C+ GYE     G C DI+EC A    C   N +C N PG++ C CP   E      GCVDI
Sbjct: 1320 GYSVRKGTTGCTDINECEIGAHNCDRQATCTNTAGSFKCNCA--PGWIGNGLKCT-DLDECSNGTH--------SCNNNAECQNTMGSYRCVCKEGYFGD-GFFCSDSDECAENSDLCENGHCLNLPGGFRCECDMGFITTADGKACEDIDECIFADICV-NGRCQNLPGLFRCECNIGYERDTSGGNCTDINEC-ADPTTCI--NGICVNIPGTYLCNCPPDFELNPTGVGCVDI 1539          

HSP 14 Score: 83.9593 bits (206), Expect = 1.773e-15
Identity = 94/299 (31.44%), Postives = 125/299 (41.81%), Query Frame = 3
Query: 2616 DINECDSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINE--CNMNPCLDKATCRNSYGSFD----------------------CFCPKGFQYSVHFGC--------------VDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            DINEC  L       +CINT GSF C C      LN +      I EC        IC       P  C ++ G   C+CP  +   +    C+D+ +  C  N  LD  TC     +F+                      C  P   ++++  G               +DIDECR+      NG+C NMIGS+RC C  G+ Y  DK   C DIDECQ +  +CQ  N  C N PGSF+C C  G  +   G C+D +EC +N        CI+  G + C C  G+    D   C
Sbjct: 1617 DINECQELPGLCQGGQCINTFGSFQCECPRGFS-LNLDTRVCEDINECEHP----GIC------GPGQCYNTIGNYTCICPADYMPVNGGNNCMDMRKSYCYRNFYLDNGTCDGEL-TFNMTKKMCCCSYNIGRAWNKPCEQCPVPSTDEFAILCGSERPGYYIDITTGRVIDIDECREIPGVCENGVCINMIGSFRCECPIGFIY-NDKLLICEDIDECQ-NGPVCQ-QNANCLNMPGSFRCECEHGFRFTPTGQCLDRNECNENPGICNPGQCIDILGSYRCVCPNGYKTTRDQSMC 1900          

HSP 15 Score: 78.9518 bits (193), Expect = 5.575e-14
Identity = 119/454 (26.21%), Postives = 174/454 (38.33%), Query Frame = 3
Query: 1947 CACKPGFVEAKGEDYCIPKESKCPSKHML-----------------DMVFIIDGSEYVTESMFNEVKFFLMETIT---QIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMK---IVTQIKYIPG-----------PKKTTL---IDAFSITGKIVLS-QCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINND----VDIPRS-IYQLSVRTTKELS-----QH------LSKVFDITCKDVNCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQY-SVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCI 3134
            C C PG+      D CI   ++C +K  +                 D  F+ + ++  TE + N   F   E +T   Q+ SS  N                L+ K+E  CD  +   I  ++  +PG           P  TT    ID   + G +  + QC   + GS + I + G  T++T+        +I+          FI  N +       PR  I Q   ++ ++L      QH      ++ +   TCK   C  G+ +    CID NEC  D   C +N  C NTPGSF C C      L+        ++EC    +    C  +            G   C CP+G+    +   C+D NEC        A+C N+ GSF C CP GF Y     GC DI+EC  ++     G C N  G Y C C  GY       C+
Sbjct: 2285 CICSPGYTRHPSGDGCI-DLNECATKPGICRNGRCENTVGSYRCKCDQGFVANPTQ--TECIDNREDFCYTEVLTTLCQMSSSNRN----------------LVTKSECCCDGGRGWGINCELCPLPGTSQYKKMCPLGPGYTTDGRDIDECRVMGNLCKNGQCVNTK-GSYKCICIPGYTTDITS----TQCVDIDECIQAPKPCNFICKNTEGSYLCSCPRGYILQEDGKSCRDLDECSTKQHNCQFLCINTIGGFTCK---CPPGFTQHHSACIDNNECSSDPNLCGSNGVCQNTPGSFNCECQRGFS-LDSNGQTCEDMDECDGNHRCQHGCQNLV-----------GGYRCSCPQGYLQHYQWNQCVDENECLNTQICGGASCHNTLGSFRCLCPSGFDYEQTSGGCADINECSTTQNPCKFG-CSNTDGGYLCGCPPGYLRAGQGHCV 2698          

HSP 16 Score: 76.6406 bits (187), Expect = 2.290e-13
Identity = 111/371 (29.92%), Postives = 152/371 (40.97%), Query Frame = 3
Query: 2532 QHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPE-FGCAPKIEECSFERKIISICDA---VTCR-------SPANCSSSNGV------EFC----------VCPRGFTYDLKEGCI---DINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDE-------------CQA------SKKLC-------QGDNVVCQNTP----GSFKCVCPVG-----LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLN--VVQPKADLV 3431
            Q+L  +F   C   N G      G NC DINEC       N  C+N PG+++C C  + E LNP   GC   I   S    +++  DA     C        S A+C  S G       E C          +CP G  +      +   DINEC   P L     C N++GSF C CP+GF  ++    C DI+EC    IC G G C N IG+Y C C   Y  V     C+D+ +             C        +KK+C       +  N  C+  P      F  +C        ++      +DIDEC++     E   CIN  G F C C  GF++N+    CE +D+  N  V Q  A+ +
Sbjct: 1472 QNLPGLFRCEC---NIGYERDTSGGNCTDINECADPTTCINGICVNIPGTYLCNCPPDFE-LNPTGVGCV-DIRSGSCYLNVLARGDASENYVCSQEIGVGVSKASCCCSEGAGWGSPCEACPHVNSTGYKTLCPGGEGFRPNPITVILEDINECQELPGLCQGGQCINTFGSFQCECPRGFSLNLDTRVCEDINECEHPGIC-GPGQCYNTIGNYTCICPADYMPVNGGNNCMDMRKSYCYRNFYLDNGTCDGELTFNMTKKMCCCSYNIGRAWNKPCEQCPVPSTDEFAILCGSERPGYYIDITTGRVIDIDECREIPGVCENGVCINMIGSFRCECPIGFIYNDKLLICEDIDECQNGPVCQQNANCL 1836          

HSP 17 Score: 75.8702 bits (185), Expect = 4.641e-13
Identity = 89/318 (27.99%), Postives = 121/318 (38.05%), Query Frame = 3
Query: 2577 CGKGYQKIGDN--CIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYR-----------------CACHNGYEYVE------------DKGCI------------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  G+Q       C D++EC  +   C NN RC+NT GSF C C+   E       C  + +EC      ++            C + +G   C+C  GF  D     C+DI+EC          C N+ GSF C C  G    +           D ++C    +     G Y                  C     Y Y E            D  C+            DI+EC     +CQ  N VC+N   ++KC C  G E    G  CVDI+EC  N+   +   C N+ G F C C KGF F+ +T  CE
Sbjct:  524 CPAGFQSTATRTECRDLDECVANGRIC-NNGRCVNTEGSFHCVCNAGFEITADGKNCQDQ-DECLIRNMCLN----------GLCINEDGSFKCICKSGFLLDTSGRMCVDIDECETPGMCMNGHCVNTEGSFRCECMAGMAVGL-----------DGRVCVDTHMRSTCYGGYNRGQCVRPLFGVVTKSECCCASTEYAYGEPCQPCPRESSGTDPCCLSSCAHKVPSAFSDINECALDPDICQ--NGVCENMQRTYKCTCNTGFEVDLSGRNCVDINECMMNSLLCDNGLCRNTPGSFTCQCFKGFRFDPETDVCE 816          

HSP 18 Score: 71.633 bits (174), Expect = 8.734e-12
Identity = 90/311 (28.94%), Postives = 135/311 (43.41%), Query Frame = 3
Query: 2604 DNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICD----AVTCRSPANCSSSNGVEF----------CVCPRGFTYDLKEGCIDINECNMNP--CLDKATCRNSYGSFDCFCPKGFQYSVHF-GCVDI---------------------------------------DECRDSKICSGNGICQNMIG-SYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGF 3356
            D C DINEC+S  C N   C+N+ GSFVC CS      +    C   ++   + + + + C+      T +S  +C ++ G  +           +C +GF       C D++EC + P  C++   C N+ GSF C CP G    V    C+D+                                       DEC ++       +C    G S+R    NG  +++     DI+EC+    LC   N  C+NT GSF+C C  G  L+  E+ C DIDEC+   + C +G TC+N+ G F C C +GF
Sbjct:  813 DVCEDINECESNPCINGD-CVNSQGSFVCTCSAASSLDSTGLECIETVKSTCWLKMVNNRCEVNINGATLKS--HCCATLGEAWNSPCAKCEKDPICGKGFARVKGNVCEDVDECQVFPGVCIN-GKCVNTQGSFFCQCPPGMTVDVSGRTCIDLRTEHCYLTHDDERCGTPIPGRHRVDACCCSVGVAWGPECDECPENGTPEFAQLCPRGPGFSHRGDFINGRPFLK-----DINECRMINTLCS--NGRCRNTIGSFRCRCDNGFALDADERNCTDIDECRISPDLCGQG-TCVNTPGDFECNCFEGF 1111          

HSP 19 Score: 67.3958 bits (163), Expect = 1.685e-10
Identity = 64/181 (35.36%), Postives = 86/181 (47.51%), Query Frame = 3
Query: 2610 CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCID 3137
            CID +EC+   C +   C+NTPGS++C C    +       C   ++EC    +I +            C ++ G   CVC  GF  T D K  C D +EC + N CL+   C N  GSF C C  GF        CVDIDEC    +C  NG C N  GS+RC C  G    ++ + C+D
Sbjct:  497 CIDDDECERNPCAH-GECVNTPGSYICQCPAGFQSTATRTECR-DLDECVANGRICNN---------GRCVNTEGSFHCVCNAGFEITADGKN-CQDQDECLIRNMCLN-GLCINEDGSFKCICKSGFLLDTSGRMCVDIDECETPGMCM-NGHCVNTEGSFRCECMAGMAVGLDGRVCVD 663          

HSP 20 Score: 58.5362 bits (140), Expect = 8.064e-8
Identity = 39/100 (39.00%), Postives = 55/100 (55.00%), Query Frame = 3
Query: 2850 CPRGFTYDLKEG-CIDINECNMNPCL-DKATCRNSYGSFDCFCPKGFQYS-VHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNG-YEYVEDKGCID 3137
            C RGF  + + G C D++EC+  P +     C N+ GSF+C CP G +++ +   C D+DEC +     G G C N +GSY C C  G Y  V+   CID
Sbjct:  241 CRRGFIPNHRTGACQDVDECHAIPGICQGGNCINTVGSFECKCPAGHKFNEISQKCEDLDECSNIPGLCGVGECSNTVGSYFCKCPQGYYTSVDGSRCID 340          

HSP 21 Score: 53.9138 bits (128), Expect = 2.140e-6
Identity = 33/87 (37.93%), Postives = 46/87 (52.87%), Query Frame = 3
Query: 3129 CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C D+DEC A   +CQG N +  NT GSF+C CP G ++ E  + C D+DEC           C N+ G + C C +G+  + D   C
Sbjct:  254 CQDVDECHAIPGICQGGNCI--NTVGSFECKCPAGHKFNEISQKCEDLDECSNIPGLCGVGECSNTVGSYFCKCPQGYYTSVDGSRC 338          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Match: fbln2 (fibulin 2 [Source:ZFIN;Acc:ZDB-GENE-060711-1])

HSP 1 Score: 127.102 bits (318), Expect = 1.528e-29
Identity = 95/284 (33.45%), Postives = 131/284 (46.13%), Query Frame = 3
Query: 2616 DINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAP----------KIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV----CPRGFTYDLKEGCIDINECN--MNPCLDKATCRNSYGSFDC-----FCPKGFQYSVH-FGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            D++EC +  C  + RC+NT GSF C          P+  C P           ++EC+  +          C     C ++ G   C     C  GFT D    C+DINEC+    PC     C N+ GSF C      C +G+  S     CVD+DEC+  S  C    IC N+ GSYRC C  GY++    K C D++EC +   +LC      C+NT GS+ C C  G  L   +K C DI+EC Q    P    C+N  G + C C +G+  + D + CE
Sbjct:  119 DVDECLTHKCLPHQRCVNTEGSFTC---------RPQITCPPGYHLKSSGCQDVDECAMRKD--------RCGKDFLCENTPGSFKCTPKQKCLSGFTQDAHGNCVDINECSSLAQPCSSSFACVNTVGSFSCQQKVLVCNRGYHTSPDGASCVDVDECQMGSHACGVGQICHNLPGSYRCDCQTGYQFDALRKVCTDVNECWSYPGRLCA---QTCENTVGSYLCTCTAGFILASDQKNCEDINECDQK---PCSQECVNMHGSYQCYCRQGYYLHEDGRTCE 379          

HSP 2 Score: 114.775 bits (286), Expect = 1.391e-25
Identity = 101/264 (38.26%), Postives = 128/264 (48.48%), Query Frame = 3
Query: 2592 QKIGDNCIDINECDSLA--CHNNSRCINTPGSFVC----YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYD-LKEGCIDINECNMNPC-LDKATCRNSYGSFDCFCPKGF-QYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDEC-QASKKLCQGDNVVCQNTPGSFKCVCPV-GLEYKEKG--CVDIDECQ-QNNTCPEGSTCINSFGGFNC 3338
            Q    NC+DINEC SLA  C ++  C+NT GSF C       N   + +P+      ++EC           +  C     C +  G   C C  G+ +D L++ C D+NEC   P  L   TC N+ GS+ C C  GF   S    C DI+EC D K CS    C NM GSY+C C  GY   ED + C DIDEC Q+  KLC      C N  GS+ C CP  G      G  C D+DEC+  ++ C    TC N  GGF C
Sbjct:  200 QDAHGNCVDINECSSLAQPCSSSFACVNTVGSFSCQQKVLVCNRGYHTSPDGASCVDVDECQM--------GSHACGVGQICHNLPGSYRCDCQTGYQFDALRKVCTDVNECWSYPGRLCAQTCENTVGSYLCTCTAGFILASDQKNCEDINEC-DQKPCSQE--CVNMHGSYQCYCRQGYYLHEDGRTCEDIDECSQSIGKLCA---FQCVNVVGSYLCACPPHGYVMSANGHTCTDVDECKVGSHNCSSEKTCFNLQGGFRC 449          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000033645.1 (pep primary_assembly:ASM223467v1:15:6710262:6717090:-1 gene:ENSORLG00000024025.1 transcript:ENSORLT00000033645.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 122.479 bits (306), Expect = 2.031e-29
Identity = 98/272 (36.03%), Postives = 126/272 (46.32%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCS----NNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTK 3377
            C  G+Q  G  C D +EC   + CH N+ C N PGS+VC C+     N  YL         ++ECS    + S     T      C +  G   C C  G+     + C+D++EC  N C   A C NS GS+ C C  GF       CVDI+EC     C    IC N +GSY CAC  G+   E + C DI+EC    K+C   N  C N+PGS+ C C  G       C D+DEC   + C   S C N  G ++C C  GF    D K
Sbjct:   79 CKSGFQGDGFQCTDNDECSRQSICHWNATCNNNPGSYVCNCNAGFKGNGNYL------CMDVDECSENPSVCSSLPGST-----GCVNLPGTYRCSCRSGYETS-GQSCVDVDECARNICSPFAKCENSLGSYKCTCNSGF-VGNGLVCVDINECNQRNECDPKAICINRMGSYECACREGF-VGEGRKCDDINEC---AKICS-SNANCTNSPGSYTCQCLKGFLGNGSVCEDVDECSTPSLCHFNSICTNLPGSYSCPCKVGFASTGDFK 332          

HSP 2 Score: 117.857 bits (294), Expect = 7.570e-28
Identity = 95/257 (36.96%), Postives = 135/257 (52.53%), Query Frame = 3
Query: 2610 CIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP-CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICS---GNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C D++EC S    C + + CINT GSF C C   + Y      C   I EC        + D   C   A C++  G   C C  GF  D  + C D +EC+    C   ATC N+ GS+ C C  GF+ + ++ C+D+DEC ++  +CS   G+  C N+ G+YRC+C +GYE    + C+D+DEC  ++ +C      C+N+ GS+KC C  G       CVDI+EC Q N C   + CIN  G + C C +GFV
Sbjct:    8 CDDMDECASQLDKCGSKASCINTLGSFNCICL--DGYTGDGKDCQ-DINEC--------LKDNGGCHPDAICTNFEGGRRCECKSGFQGDGFQ-CTDNDECSRQSICHWNATCNNNPGSYVCNCNAGFKGNGNYLCMDVDECSENPSVCSSLPGSTGCVNLPGTYRCSCRSGYE-TSGQSCVDVDEC--ARNICS-PFAKCENSLGSYKCTCNSGFVGNGLVCVDINECNQRNECDPKAICINRMGSYECACREGFV 248          

HSP 3 Score: 63.929 bits (154), Expect = 4.939e-10
Identity = 44/123 (35.77%), Postives = 59/123 (47.97%), Query Frame = 3
Query: 3006 CVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNND 3371
            C D+DEC      C     C N +GS+ C C +GY   + K C DI+EC      C  D  +C N  G  +C C  G +     C D DEC + + C   +TC N+ G + C CN GF  N +
Sbjct:    8 CDDMDECASQLDKCGSKASCINTLGSFNCICLDGYT-GDGKDCQDINECLKDNGGCHPD-AICTNFEGGRRCECKSGFQGDGFQCTDNDECSRQSICHWNATCNNNPGSYVCNCNAGFKGNGN 128          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000027703.1 (latent transforming growth factor beta binding protein 2 [Source:NCBI gene;Acc:101168829])

HSP 1 Score: 125.176 bits (313), Expect = 4.037e-28
Identity = 91/265 (34.34%), Postives = 125/265 (47.17%), Query Frame = 3
Query: 2607 NCIDINECDSLACHNNSRCINTPGSFVCYC-SNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDC-FCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNC-TCNKGFVFNNDTKFCE 3386
            NCID+NECD   C     CIN+ GS+ C C S   + +         I ECS   K  +           NC ++ G   C C  GF    ++ C D++EC          C N+ GSF C  C  G +  ++  CVD++EC +  +C G G C N  GSYRC C +GY+   D  C D++EC   +    G   +C N  G+ +C C  G +     K C D++EC   N CP G  CIN+ G + C  C  GF  + D   CE
Sbjct:  871 NCIDVNECDEDPCEGKGHCINSYGSYTCQCHSGYSQVITQNRKFCQDINECSMPNKCQN----------GNCVNTEGSYTCECNSGFAKSWRDQCEDLDECKDPSSCPSGICINTLGSFQCQACSPGLRL-INERCVDVNECLNQTVC-GRGRCVNTQGSYRCNCFHGYKLSPDNVCQDVEECVLPEVCNHG---LCVNLDGTHECTCNHGYQVTPDRKSCEDVNECATGNACPVG-ICINTAGSYTCQNCRPGFGPSADGLRCE 1119          

HSP 2 Score: 115.546 bits (288), Expect = 3.848e-25
Identity = 99/279 (35.48%), Postives = 135/279 (48.39%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF--TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCA-CHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV-CPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNC-TCNKGFVFNNDTKFC 3383
            C  G + I + C+D+NEC +       RC+NT GS+ C C +  + L+P+  C   +EEC     +  +C+         C + +G   C C  G+  T D K  C D+NEC          C N+ GS+ C  C  GF  S     C DIDEC    +C G G+C N  GS+ C  C  GY   ++ + C DIDECQ+      G   +C N+ GS+ C  CP G      G  C DIDEC    TCP+G TC N+ G + C  C  GF  + D + C
Sbjct:  984 CSPGLRLINERCVDVNECLNQTVCGRGRCVNTQGSYRCNCFHGYK-LSPDNVCQ-DVEECV----LPEVCNH------GLCVNLDGTHECTCNHGYQVTPDRKS-CEDVNECATGNACPVGICINTAGSYTCQNCRPGFGPSADGLRCEDIDECARGDMCLG-GVCANTEGSFICTRCTAGYRVSLDQQSCEDIDECQSISTCANG---ICLNSEGSYSCEHCPAGYRVSHDGEFCKDIDECALPTTCPKG-TCTNTEGSYMCVVCQPGFRVSEDGQQC 1244          

HSP 3 Score: 112.849 bits (281), Expect = 2.151e-24
Identity = 102/290 (35.17%), Postives = 135/290 (46.55%), Query Frame = 3
Query: 2538 LSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDC-FCPKGFQYSVHF-GCVDIDECRDSKICSGNGICQNMIGSYRC-ACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV-CPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            ++     TC++   G G    G  C DI+EC          C NT GSF+C        ++ +      I+EC    + IS C    C    N   S   E C      ++D  E C DI+EC +     K TC N+ GS+ C  C  GF+ S     C D++EC  S +C G  +C N  GSY C +C  G++  ED   C DI+ECQ       G   VC NT GSF C+ C  G      G  C D+DEC  +N C  G  CIN++G F C C  GF F+ DT  C+
Sbjct: 1094 INTAGSYTCQNCRPGFGPSADGLRCEDIDECARGDMCLGGVCANTEGSFICTRCTAGYRVSLDQQSCEDIDEC----QSISTCANGIC---LNSEGSYSCEHCPAGYRVSHD-GEFCKDIDECALPTTCPKGTCTNTEGSYMCVVCQPGFRVSEDGQQCNDVNECLGSSVCPGQ-MCLNTQGSYTCRSCQTGFQPSEDGHTCQDINECQTPDVCPTG---VCTNTEGSFSCMTCAPGFTVAPNGLSCEDVDECNNDNVCVRGQ-CINTYGSFLCLCEAGFKFSVDTADCD 1370          

HSP 4 Score: 103.219 bits (256), Expect = 1.992e-21
Identity = 76/225 (33.78%), Postives = 109/225 (48.44%), Query Frame = 3
Query: 2733 CAPKIEECSFERKIISI--CDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG---CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C P  +  + +   I +  CD   C    +C +S G   C C  G++  + +    C DINEC+M        C N+ GS+ C C  GF  S    C D+DEC+D   C  +GIC N +GS++C AC  G   + ++ C+D++EC       +G    C NT GS++C C  G +      C D++EC     C  G  C+N  G   CTCN G+    D K CE
Sbjct:  859 CDPGFKLSALQTNCIDVNECDEDPCEGKGHCINSYGSYTCQCHSGYSQVITQNRKFCQDINECSMPNKCQNGNCVNTEGSYTCECNSGFAKSWRDQCEDLDECKDPSSCP-SGICINTLGSFQCQACSPGLRLINER-CVDVNECLNQTVCGRGR---CVNTQGSYRCNCFHGYKLSPDNVCQDVEECVLPEVCNHG-LCVNLDGTHECTCNHGYQVTPDRKSCE 1077          

HSP 5 Score: 82.4185 bits (202), Expect = 4.702e-15
Identity = 86/294 (29.25%), Postives = 132/294 (44.90%), Query Frame = 3
Query: 2571 VNCGKGYQ--KIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV-CPRGFTYDLKE-GCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRD--SKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG---CVDIDECQQNNTCPEGSTCINSFGGFNCTC-NKGFVFNNDTKFCELDKTLNVVQPK 3419
            V C  G++  + G  C D+NEC   +      C+NT GS+ C         + +      I EC    +   +C    C      +++ G   C+ C  GFT       C D++ECN +    +  C N+YGSF C C  GF++SV    C D DEC++  S +C G   CQN IGSY C            G ++ D  +   ++  G++  C+NT  SF C C  G          CVD++EC+ ++     + C N  G F C C N    F+  T  C   +++ ++ P+
Sbjct: 1229 VVCQPGFRVSEDGQQCNDVNECLGSSVCPGQMCLNTQGSYTCRSCQTGFQPSEDGHTCQDINEC----QTPDVCPTGVC------TNTEGSFSCMTCAPGFTVAPNGLSCEDVDECNNDNVCVRGQCINTYGSFLCLCEAGFKFSVDTADCDDQDECKEFGSSVC-GAWRCQNTIGSYSC----------QSGILEEDWDKCDSEINCGEHGFCENTNSSFHCNCDKGYTNPAGDVTQCVDVNECETSSALCGEALCENYDGSFLCICPNDNEEFDPITNRC---RSMAMIDPE 1498          

HSP 6 Score: 81.2629 bits (199), Expect = 8.772e-15
Identity = 82/272 (30.15%), Postives = 118/272 (43.38%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKII--SICDAVTCRSPANCSSSNGVEFC-VCPRGF-TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            +C  GY+    G+ C DI+EC          C NT GS++C          P F  +   ++C+   + +  S+C    C       ++ G   C  C  GF   +    C DINEC          C N+ GSF C  C  GF  + +   C D+DEC +  +C   G C N  GS+ C C  G+++ V+   C D DEC+     +C      CQNT GS+ C   +  E       D D+C     C E   C N+   F+C C+KG+ 
Sbjct: 1188 HCPAGYRVSHDGEFCKDIDECALPTTCPKGTCTNTEGSYMCV------VCQPGFRVSEDGQQCNDVNECLGSSVCPGQMCL------NTQGSYTCRSCQTGFQPSEDGHTCQDINECQTPDVCPTGVCTNTEGSFSCMTCAPGFTVAPNGLSCEDVDECNNDNVCV-RGQCINTYGSFLCLCEAGFKFSVDTADCDDQDECKEFGSSVCGA--WRCQNTIGSYSCQSGILEE-------DWDKCDSEINCGEHGFCENTNSSFHCNCDKGYT 1437          

HSP 7 Score: 65.0846 bits (157), Expect = 7.059e-10
Identity = 86/300 (28.67%), Postives = 129/300 (43.00%), Query Frame = 3
Query: 2574 NCGKGY-QKIGD--NCIDINECD-SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIG------SYRCACHNGYEYVEDKGCIDIDECQA-SKKLCQGDNVVCQNTPGSFKCVCPVGLEYK--EKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQPKADL 3428
            NC KGY    GD   C+D+NEC+ S A    + C N  GSF+C C N+ E  +P                I + C ++    P +        + V P  FT + KE   ++N+ N  N  L + T +      +C C  G  +  +    +I  C        N +C +  G      S R +  +G  Y      ID DEC+    ++C+  N  C N   ++ C C  G  Y      CVD DECQ  NTC  G+ C+N+ G FNC C+   V ++  + C  L+ T   ++   D+
Sbjct: 1431 NCDKGYTNPAGDVTQCVDVNECETSSALCGEALCENYDGSFLCICPNDNEEFDP----------------ITNRCRSMAMIDPEHI-------YPVIP--FTDERKECYYNLNDANYCNNVLSRNTTKQ-----ECCCTAGAGWGDN---CEIHTCPLPGKDDYNQLCPHGSGILPLSDSLRQSHGDGLPY------IDADECEMFGSEICK--NGQCSNIFSTYTCFCRSGYYYDNIRFECVDFDECQFGNTCENGA-CVNTAGSFNCFCSPPLVLDDSQRHCISLNSTEETIEYGQDV 1688          
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000000411.2 (latent-transforming growth factor beta-binding protein 1 [Source:NCBI gene;Acc:101168560])

HSP 1 Score: 124.405 bits (311), Expect = 6.263e-28
Identity = 98/277 (35.38%), Postives = 143/277 (51.62%), Query Frame = 3
Query: 2577 CGKGYQKIGDN--CIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCV-CPRGFTYDLKEG-CIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRC-ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE 3386
            C  GY+       C D++EC    C +NSRC NTPGS+ C C +   Y      C   ++EC          D + C     C ++ G   C  C  G  Y L  G C DI+EC  +PC   A C N+ GS+ C C  G++   +  C D+DEC +   C+G  +C N  GSYRC +C +GY+ + ++ C D+DEC+ S   C+ D   C NT GSF+C C  G +    G  C DI+EC + + C  G  C+N+ G + C C++GF  +++   C+
Sbjct:  909 CESGYEHGPQRTTCQDVDECLQNPC-SNSRCENTPGSYRCVCRHG--YRLSGNTCT-DVDECV---------DPLRCPG-QECVNTLGSYRCTSCQPG--YGLLNGLCTDIDECRQSPC-SNARCENTPGSYRCVCRPGYRLQ-NDTCADMDECAEPSRCNGQ-MCVNSAGSYRCVSCRSGYK-LTNRQCTDVDECE-SAAACEADRR-CVNTAGSFRCECLPGYQASSLGRQCRDINECLEGDFCFSGGECLNTPGSYMCVCSQGFTLSSNGTACK 1163          

HSP 2 Score: 119.398 bits (298), Expect = 2.655e-26
Identity = 95/275 (34.55%), Postives = 129/275 (46.91%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINEC-DSLACHNNSRCINTPGSFVC-YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCF-CPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVE-DKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG--CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC 3383
            C  GY+  G+ C D++EC D L C     C+NT GS+ C  C      LN   G    I+EC       S C      S A C ++ G   CVC  G+     + C D++EC      +   C NS GS+ C  C  G++ + +  C D+DEC  +  C  +  C N  GS+RC C  GY+     + C DI+EC        G    C NTPGS+ CVC  G      G  C D+DEC +   C +G  C+N+ G F+C C  GF  N +   C
Sbjct:  949 CRHGYRLSGNTCTDVDECVDPLRCPGQE-CVNTLGSYRCTSCQPGYGLLN---GLCTDIDECR-----QSPC------SNARCENTPGSYRCVCRPGYRLQ-NDTCADMDECAEPSRCNGQMCVNSAGSYRCVSCRSGYKLT-NRQCTDVDECESAAACEADRRCVNTAGSFRCECLPGYQASSLGRQCRDINECLEGDFCFSGGE--CLNTPGSYMCVCSQGFTLSSNGTACKDVDECLKPGVCSDGR-CVNTDGSFHCRCANGFASNPERTAC 1203          

HSP 3 Score: 109.383 bits (272), Expect = 2.425e-23
Identity = 91/278 (32.73%), Postives = 129/278 (46.40%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCY-CSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-TYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVH-FGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV--CPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCN-KGFVFNNDTKFC 3383
            C  GY+   D C D++EC   +  N   C+N+ GS+ C  C +  +  N +      ++EC           A  C +   C ++ G   C C  G+    L   C DINEC   + C     C N+ GS+ C C +GF  S +   C D+DEC    +CS +G C N  GS+ C C NG+    E   C+D+DEC +S     G    C+NT GS++C+  C  G   +  G C D DEC         + C N  GGF C C+  G VF   ++ C
Sbjct: 1027 CRPGYRLQNDTCADMDECAEPSRCNGQMCVNSAGSYRCVSCRSGYKLTNRQ---CTDVDECE---------SAAACEADRRCVNTAGSFRCECLPGYQASSLGRQCRDINECLEGDFCFSGGECLNTPGSYMCVCSQGFTLSSNGTACKDVDECLKPGVCS-DGRCVNTDGSFHCRCANGFASNPERTACLDVDECISSNGSVCGSQR-CENTIGSYRCLTSCEAGYRVQPDGSCADEDECASQPGVCGTARCENLKGGFVCRCDITGQVFEATSRRC 1290          

HSP 4 Score: 106.686 bits (265), Expect = 1.618e-22
Identity = 78/228 (34.21%), Postives = 106/228 (46.49%), Query Frame = 3
Query: 2571 VNCGKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVT---CRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSV-HFGCVDIDEC--RDSKICSGNGICQNMIGSYRC--ACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVC 3224
            V+C  GY+     C D++EC+S  AC  + RC+NT GSF C              C P  +  S  R+   I + +    C S   C ++ G   CVC +GFT       C D++EC          C N+ GSF C C  GF  +     C+D+DEC   +  +C G+  C+N IGSYRC  +C  GY    D  C D DEC +   +C      C+N  G F C C
Sbjct: 1065 VSCRSGYKLTNRQCTDVDECESAAACEADRRCVNTAGSFRCE-------------CLPGYQASSLGRQCRDINECLEGDFCFSGGECLNTPGSYMCVCSQGFTLSSNGTACKDVDECLKPGVCSDGRCVNTDGSFHCRCANGFASNPERTACLDVDECISSNGSVC-GSQRCENTIGSYRCLTSCEAGYRVQPDGSCADEDECASQPGVC--GTARCENLKGGFVCRC 1276          

HSP 5 Score: 69.707 bits (169), Expect = 3.171e-11
Identity = 42/110 (38.18%), Postives = 58/110 (52.73%), Query Frame = 3
Query: 3048 GNGICQNMIGS-YRCACHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCT-CNKGFVFNN 3368
            G G C ++ G  + C C +GYE+   +  C D+DEC   +  C   N  C+NTPGS++CVC  G       C D+DEC     CP G  C+N+ G + CT C  G+   N
Sbjct:  893 GRGHCLDLPGGKHSCLCESGYEHGPQRTTCQDVDECL--QNPC--SNSRCENTPGSYRCVCRHGYRLSGNTCTDVDECVDPLRCP-GQECVNTLGSYRCTSCQPGYGLLN 997          

HSP 6 Score: 53.1434 bits (126), Expect = 3.194e-6
Identity = 41/106 (38.68%), Postives = 56/106 (52.83%), Query Frame = 3
Query: 3012 DIDECR--DSKICSGNGICQNMIGSYRCACHNGYEYVED-KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINS 3320
            D+DEC+    ++C  NG+C N I  YRC C++GY Y      C+D DEC+  ++ C G   VC NT GS+ C CP  L         +DE Q+N        C+NS
Sbjct: 1420 DVDECKRFQPQVCK-NGVCVNNIPGYRCYCYSGYVYNSTLLECVDHDECE--EETCVGG--VCVNTVGSYYCTCPTHLV--------LDETQRN--------CVNS 1504          
BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Match: SMESG000034758.1 (SMESG000034758.1)

HSP 1 Score: 3214.86 bits (8334), Expect = 0.000e+0
Identity = 1616/1617 (99.94%), Postives = 1617/1617 (100.00%), Query Frame = 3
Query:  225 MIINNCIWLLITVTFTGILQTESRLYYDLKRFPRIADTQYFMSSSGEIRIKRQSDVTSSLEVVDPYCTIGDLDGKVCNEYLPMNKDKSCPSDSIPKKNRCVRFRNPRCPSRYTNVDGKCILDRGCDLKEYDQIGRNCYEKCQWSHRRYYSNLCISEKPGCPLQFRWNGTDHCILSECPTLLDQTEDGLYCQTRCPPYFEKINSSTCESNRTICPIGYDKEGTYCIQKTCERGLLLNGFCLDTICKTNYEFDGKVCKKCLTSTHSYCSAMDWTCPMGLIEIGSGPHMQCVMICPKRYAQFGKSCYDISVTGCPEGYLHFEDNTCIEESKCPDGFEIFGTECVRKLTYVGFIINSKEVQLCPTDYIEYQGKCFKLTDNACPDGTLAIEDSHYCVSTCPQGFNNVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQVLDLLSFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCPVDYDTDAQFCKRRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSVVCGVNKELQDCASSNGGECNTKCACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMXXXXXXXXXXXXXXDMYLRHGQYNSSKLVIFITATKPNSYDKSXXXXXXXXXXXXXXDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDKLYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCTLNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCARQVTSQCIFDGKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIKDKIXXXXXXXXXXXXXMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI 5075
            MIINNCIWLLITVTFTGILQTESRLYYDLKRFPRIADTQYFMSSSGEIRIKRQSDVTSSLEVVDPYCTIGDLDGKVCNEYLPMNKDKSCPSDSIPKKNRCVRFRNPRCPSRYTNVDGKCILDRGCDLKEYDQIGRNCYEKCQWSHRRYYSNLCISEKPGCPLQFRWNGTDHCILSECPTLLDQTEDGLYCQTRCPPYFEKINSSTCESNRTICPIGYDKEGTYCIQKTCERGLLLNGFCLDTICKTNYEFDGKVCKKCLTSTHSYCSAMDWTCPMGLIEIGSGPHMQCVMICPKRYAQFGKSCYDISVTGCPEGYLHFEDNTCIEESKCPDGFEIFGTECVRKLTYVGFIINSKEVQLCPTDYIEYQGKCFKLTDNACPDGTLAIEDSHYCVSTCPQGFNNVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQVLDLLSFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCPVDYDTDAQFCKRRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSVVCGVNKELQDCASSNGGECNTKCACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMGGKSLLSSGLKSAKDMYLRHGQYNSSKLVIFITATKPNSYDKSEIIEIKEQVKEIKKDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDKLYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCTLNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCARQVTSQCIFDGKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIKDKITGEKTKLTGGGGLMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDG+AKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI
Sbjct:    1 MIINNCIWLLITVTFTGILQTESRLYYDLKRFPRIADTQYFMSSSGEIRIKRQSDVTSSLEVVDPYCTIGDLDGKVCNEYLPMNKDKSCPSDSIPKKNRCVRFRNPRCPSRYTNVDGKCILDRGCDLKEYDQIGRNCYEKCQWSHRRYYSNLCISEKPGCPLQFRWNGTDHCILSECPTLLDQTEDGLYCQTRCPPYFEKINSSTCESNRTICPIGYDKEGTYCIQKTCERGLLLNGFCLDTICKTNYEFDGKVCKKCLTSTHSYCSAMDWTCPMGLIEIGSGPHMQCVMICPKRYAQFGKSCYDISVTGCPEGYLHFEDNTCIEESKCPDGFEIFGTECVRKLTYVGFIINSKEVQLCPTDYIEYQGKCFKLTDNACPDGTLAIEDSHYCVSTCPQGFNNVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQVLDLLSFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCPVDYDTDAQFCKRRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKNSVVCGVNKELQDCASSNGGECNTKCACKPGFVEAKGEDYCIPKESKCPSKHMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGPENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQCNGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMGGKSLLSSGLKSAKDMYLRHGQYNSSKLVIFITATKPNSYDKSEIIEIKEQVKEIKKDGRLLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRLIKCPEGFIFLDKVCQYKTTKAIYCKWDDKLYALNSTWTSSCAVYACRENGVVVLETNCMGFDGDCYKEDEFFPCTLNGNLNWRCKCKIVQYTDEETKKSQQRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNRWCAHHMYYQRGLYSNCARQVTSQCIFDGKIYKEGDKWSVGCNVVVCDSGKPYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIKDKITGEKTKLTGGGGLMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGVAKVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNEGGKIERKVVDTKKIVNI 1617          
BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Match: SMESG000021248.1 (SMESG000021248.1)

HSP 1 Score: 129.798 bits (325), Expect = 1.448e-29
Identity = 105/309 (33.98%), Postives = 151/309 (48.87%), Query Frame = 3
Query: 2574 NCGKGYQ--KIGDNCIDINECD---SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPAN------CSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDC--FCPKGFQYSVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCP--------------------VGLEY-KEKGCVDIDECQQNNTCPEG--STCINSFGGFNCTCNKGFVFNNDTKFC 3383
            NC  G+     G +C DINEC    S     N  CINTPGS++C          P+  CAP +   SF++  ++  D   C    N      C +++G  +C+C  G+   +K  CIDI+EC++    C     CRN+ GSF C   C  G+Q  ++  C D+DEC      CS +  C N+ GSYRC C++G+ Y     CID++ECQ +   C      C+NT GS++C+CP                     G  +  ++GC+DIDEC  + T P      CIN++G ++C C  GF  N  T  C
Sbjct: 3680 NCRSGFMLSSDGKSCQDINECSKNISPCSEPNHECINTPGSYMC---------KPK--CAPGLRR-SFDK--LTCGDINECVEKPNICDSQLCINTHGSYYCLCRHGYK-KMKNSCIDIDECSLGIAKCGLHQKCRNTPGSFFCENLCYDGYQL-INEQCYDLDECSSGTANCSKSSTCTNLPGSYRCECNDGF-YTSGSTCIDVNECQTNSSQCIYG---CKNTYGSYECICPPNFIQSRDKKSCLKKHEICKTGFTWTSDRGCIDIDECT-SYTNPHSCQYQCINTYGSYHCLCPPGFSLNPKTMKC 3967          

HSP 2 Score: 120.939 bits (302), Expect = 7.256e-27
Identity = 99/282 (35.11%), Postives = 138/282 (48.94%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA---CHNNSRCINTPGSFVC--YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQA--SKKLCQGDNVVCQNTPGSFKCVCPVG--LEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNCT---CNKGFVFNNDTKFC 3383
            C  GY+K+ ++CIDI+EC SL    C  + +C NTPGSF C   C +  + +N +  C   ++ECS             C   + C++  G   C C  GF Y     CID+NEC  N       C+N+YGS++C CP  F  S           RD K C    + ++ I      C  G+ +  D+GCIDIDEC +  +   CQ     C NT GS+ C+CP G  L  K   C+D+DEC+ N   C E   CIN+ G ++C    C K + F+   K C
Sbjct: 3769 CRHGYKKMKNSCIDIDEC-SLGIAKCGLHQKCRNTPGSFFCENLCYDGYQLINEQ--CY-DLDECS--------SGTANCSKSSTCTNLPGSYRCECNDGF-YTSGSTCIDVNECQTNSSQCIYGCKNTYGSYECICPPNFIQS-----------RDKKSC----LKKHEI------CKTGFTWTSDRGCIDIDECTSYTNPHSCQYQ---CINTYGSYHCLCPPGFSLNPKTMKCLDVDECRTNLGICSEKKLCINTPGNYSCIEPKCPKSYHFDIKLKAC 4013          

HSP 3 Score: 72.4034 bits (176), Expect = 3.587e-12
Identity = 69/186 (37.10%), Postives = 87/186 (46.77%), Query Frame = 3
Query: 2850 CPRGFTY-DLKEGCIDINECNMNPCLDK--ATCRNSYGSFDCFCPKGFQYSVHF-GCVDIDEC-RDSKICS-GNGICQNMIGSYRCA--CHNGYEYVEDK-GCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN-NTCPEGSTCINSFGGFNC--TCNKGFVFNND 3371
            CP GFT    +  C D+NEC  N  L K    C N+   F C C  GF  S     C DI+EC ++   CS  N  C N  GSY C   C  G     DK  C DI+EC     +C  D+ +C NT GS+ C+C  G +  +  C+DIDEC      C     C N+ G F C   C  G+   N+
Sbjct: 3639 CPPGFTLIKSQRFCEDVNECK-NAKLHKCDQICENTVPRFKCNCRSGFMLSSDGKSCQDINECSKNISPCSEPNHECINTPGSYMCKPKCAPGLRRSFDKLTCGDINECVEKPNIC--DSQLCINTHGSYYCLCRHGYKKMKNSCIDIDECSLGIAKCGLHQKCRNTPGSFFCENLCYDGYQLINE 3821          
BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Match: SMESG000032548.1 (SMESG000032548.1)

HSP 1 Score: 115.931 bits (289), Expect = 1.242e-25
Identity = 97/296 (32.77%), Postives = 133/296 (44.93%), Query Frame = 3
Query: 2541 SKVFDITCKDVNCGKGYQKIGDN-CIDINEC-----DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAV--------TCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM----NPCLD-KATCRNSYGSFDCFCPKGFQYSVHF--GCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQ-------QNNTCPEGSTCINSFGGFNC 3338
            S+ FD +     C  GY++  D+ C D++EC     D+L C +   CIN PGS+ C              C P +    F   + S C  V         C    +C +  G   C+C RG+       C D+NEC +      C D +A C N+ GS+ C CPKGF++S +    C DI+EC  +  C     C N +GSY+C C  GY   +    C DIDEC    + C   N +C N PG ++C CP G     K  C DIDEC        + +    G  CIN  G + C
Sbjct:  554 SQQFDYSVGKCMCRDGYRQNQDSICTDVDECAENGGDNL-CGSLYICINHPGSYACI---------QRAECPPGM----FRSSMYSNCQDVDECETGEGKCPQNMHCVNVVGSYKCMCDRGYILTSNNTCADVNECLLFRPEESCPDLEARCVNTAGSYKCQCPKGFEWSDYPIKKCTDINECLAANPCGKKHQCVNTVGSYKCKCFQGYRLSISGDTCEDIDECLLEPRKCL--NGICVNVPGRYECKCPPGFAADRKEICQDIDECSTSEGVCSRESDFQNGRLCINLLGYYKC 833          

HSP 2 Score: 108.997 bits (271), Expect = 1.671e-23
Identity = 100/328 (30.49%), Postives = 145/328 (44.21%), Query Frame = 3
Query: 2562 CKDVNCGKGYQKIGDNCIDINECDSLACHNNSRC------INTPGSFVC-----------------YCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNP----CLDKATCRNSYGSFDCF----CPKG-FQYSVHFGCVDIDECRDSK-ICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQ--ASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE---KGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCE-LDKTLNVVQPKADL 3428
            CKD   G  ++K   +C DI+EC       N RC       N PGS+ C                  C++ +E    +F C P  +E          C A  C        S G   C+C  G+  +    C D++EC  N     C     C N  GS+ C     CP G F+ S++  C D+DEC   +  C  N  C N++GSY+C C  GY    +  C D++EC     ++ C      C NT GS+KC CP G E+ +   K C DI+EC   N C +   C+N+ G + C C +G+  +     CE +D+ L  ++P+  L
Sbjct:  454 CKD---GFYFKKETRSCEDIDECTMNVGWYNGRCKETDVCFNLPGSYDCIPKKKCRPGTVLAQNGIACNDIDECKQMKFRCEP--DEICVNTYSSYHCVASPCAPSQQFDYSVGK--CMCRDGYRQNQDSICTDVDECAENGGDNLCGSLYICINHPGSYACIQRAECPPGMFRSSMYSNCQDVDECETGEGKCPQNMHCVNVVGSYKCMCDRGYILTSNNTCADVNECLLFRPEESCPDLEARCVNTAGSYKCQCPKGFEWSDYPIKKCTDINECLAANPCGKKHQCVNTVGSYKCKCFQGYRLSISGDTCEDIDECL--LEPRKCL 772          

HSP 3 Score: 106.301 bits (264), Expect = 1.133e-22
Identity = 84/231 (36.36%), Postives = 113/231 (48.92%), Query Frame = 3
Query: 2607 NCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT---YDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDN-----VVCQNTPGSFKCV---CPVGLEYKEKG 3254
            NC D++EC++    C  N  C+N  GS+ C C  +  Y+         + EC   R   S  D       A C ++ G   C CP+GF    Y +K+ C DINEC   NPC  K  C N+ GS+ C C +G++ S+    C DIDEC        NGIC N+ G Y C C  G+     + C DIDEC  S+ +C  ++      +C N  G +KCV   CPVG   KE G
Sbjct:  626 NCQDVDECETGEGKCPQNMHCVNVVGSYKCMC--DRGYILTSNNTCADVNECLLFRPEESCPDL-----EARCVNTAGSYKCQCPKGFEWSDYPIKK-CTDINECLAANPCGKKHQCVNTVGSYKCKCFQGYRLSISGDTCEDIDECLLEPRKCLNGICVNVPGRYECKCPPGFAADRKEICQDIDECSTSEGVCSRESDFQNGRLCINLLGYYKCVNSSCPVGYTKKETG 848          

HSP 4 Score: 95.5153 bits (236), Expect = 2.540e-19
Identity = 72/196 (36.73%), Postives = 99/196 (50.51%), Query Frame = 3
Query: 2850 CPRGFTYD-LKEGCIDINEC-NMNPCLDK-ATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRC--ACHNGYEYVED---KGCIDIDECQASKKLCQGDNVVCQNTPGSFKC---VCPVGLEYKEKG-----------------CVDIDECQQ-----NNTCPEGSTCINSFGGFNC 3338
            C +GF ++   E C DINEC   N C +K   C N+ GSF C C KGF++S    C DIDEC +  ICS +  C+N +GSY+C  +   G+ + ED     C+D+DEC+A  ++C G  + C N  GS +C    CP+ ++   KG                 C DIDEC       N  C E   C N  G ++C
Sbjct:  305 CSKGFLFNQFLEICGDINECETANACANKWEICVNTVGSFRCDCQKGFKHSKLGICEDIDECMNVNICSDDKKCKNNVGSYKCIRSIPCGFGWKEDPDTNDCVDVDECEAFPEIC-GPGMKCVNIRGSHRCEDDECPINMKRNNKGICSRCKDGFYFKKETRSCEDIDECTMNVGWYNGRCKETDVCFNLPGSYDC 499          

HSP 5 Score: 92.0485 bits (227), Expect = 2.690e-18
Identity = 87/323 (26.93%), Postives = 135/323 (41.80%), Query Frame = 3
Query: 2565 KDVNCGKGYQKIGD--NCIDINECDSLA--CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMN------PCLDKATCRNSYGSFDCF----CPKGFQYSVH-FGCVDIDECRDSKI-CSGNGICQNMIGSY------------------RCACHNGYEYVEDKGCIDIDEC--QASKKLCQGDNVVCQNTPGSFKCV----CPVGLEYKE--KGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTL 3401
            + + CG G+++  D  +C+D++EC++    C    +C+N  GS  C                 + +EC                 P N   +N      C  GF +  +   C DI+EC MN       C +   C N  GS+DC     C  G   + +   C DIDEC+  K  C  + IC N   SY                  +C C +GY   +D  C D+DEC       LC G   +C N PGS+ C+    CP G+        C D+DEC+     CP+   C+N  G + C C++G++  ++    ++++ L
Sbjct:  389 RSIPCGFGWKEDPDTNDCVDVDECEAFPEICGPGMKCVNIRGSHRC-----------------EDDEC-----------------PINMKRNNKGICSRCKDGFYFKKETRSCEDIDECTMNVGWYNGRCKETDVCFNLPGSYDCIPKKKCRPGTVLAQNGIACNDIDECKQMKFRCEPDEICVNTYSSYHCVASPCAPSQQFDYSVGKCMCRDGYRQNQDSICTDVDECAENGGDNLC-GSLYICINHPGSYACIQRAECPPGMFRSSMYSNCQDVDECETGEGKCPQNMHCVNVVGSYKCMCDRGYILTSNNTCADVNECL 676          

HSP 6 Score: 75.485 bits (184), Expect = 3.632e-13
Identity = 88/311 (28.30%), Postives = 124/311 (39.87%), Query Frame = 3
Query: 2526 LSQHLSKVFDITCKDVNCGKGY--QKIGDNCIDINECDSL-ACHNNSR-CINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKATCRNSYGSFDCF--CPKGFQYSVHF---GCVDIDECRD-SKICSGNGICQNMIGSYRCA------------------CHNGYEY-VEDKGCIDIDECQASKKLCQG---DNVVCQNTPGSFKCV----CPVGLEYKEKG--CVDIDECQQNN-TCPEGSTCINSFGGFNC 3338
            L  + SK   +    V C KG+   +  + C DINEC++  AC N    C+NT GSF C C                     F+   + IC+                                  DI+EC N+N C D   C+N+ GS+ C    P GF +        CVD+DEC    +IC     C N+ GS+RC                   C +G+ +  E + C DIDEC  +     G   +  VC N PGS+ C+    C  G    + G  C DIDEC+Q    C     C+N++  ++C
Sbjct:  288 LHPNTSKCVPVVKPKVPCSKGFLFNQFLEICGDINECETANACANKWEICVNTVGSFRCDCQK------------------GFKHSKLGICE----------------------------------DIDECMNVNICSDDKKCKNNVGSYKCIRSIPCGFGWKEDPDTNDCVDVDECEAFPEICGPGMKCVNIRGSHRCEDDECPINMKRNNKGICSRCKDGFYFKKETRSCEDIDECTMNVGWYNGRCKETDVCFNLPGSYDCIPKKKCRPGTVLAQNGIACNDIDECKQMKFRCEPDEICVNTYSSYHC 546          
BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Match: SMESG000076582.1 (SMESG000076582.1)

HSP 1 Score: 116.316 bits (290), Expect = 1.712e-25
Identity = 92/281 (32.74%), Postives = 139/281 (49.47%), Query Frame = 3
Query: 2574 NCGKGYQK------IGD----NCIDINECDSL--ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIE-ECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFT-YDLKEGCIDINECNMNP----CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRD-SKICSGNGICQNMIGSYRCACHNGYEYVEDKG--CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKG 3353
            NC  GY+       IGD     C DI+EC +   ACH  + C N  G++VC C     YL  +     + + EC+ E    ++ +   C +  N  +      C C  G+      + C++ NEC +NP    C++ A C ++ GS++C C  GF+      C +I+EC + S  CS N +CQ+    + C C  G+++ ++    C DIDEC          + +C N PGSF+CVCP G   +   C DI+EC   N+C + S C N+  GFNC+C  G
Sbjct: 4819 NCTNGYEDSNQMECIGDVCYKKCSDIDECVTRKSACHKFAECTNNDGAYVCTC---PSYLTGDGKTTCEDQNECALETHTCNL-NTSYCENLDNTVNVLAPFQCHCFNGYVKIPGTQICLNKNEC-LNPVENDCVENAICIDTEGSYECRCKSGFKQLPSGKCENINECEEKSDNCSINSVCQDKADGFSCQCKPGFKWSDETNLNCEDIDECTNPTSCSSMPHSICMNNPGSFECVCPHGFSLQGGYCNDINECLIPNSCGQNSVCANTVSGFNCSCANG 5094          

HSP 2 Score: 112.849 bits (281), Expect = 2.095e-24
Identity = 104/311 (33.44%), Postives = 136/311 (43.73%), Query Frame = 3
Query: 2577 CGKGYQK-----IGDNCIDINECD-SLACHNNSRCINTPGSFVCYCSNN---------------EEY--LNPEFGCAPKIE----ECS----FERKIISIC---------DAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC---NMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI--CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCP------EGSTCINSFGGFNCTCNKGF 3356
            C  G+Q+     + D+C DI+EC  S +C  N  C NT GSF C C +                E+Y   + +  C   +E    EC     F    I+ C            +C   A C   N    C+CP GF  D    C DI+EC   N + C     C NS G ++C C  G  Y V   CVDIDEC  ++   CS N ICQN  G+Y C C  G+ Y +   C DIDEC+     C  +N +C N PG+F C C  G       C D DEC++    P      E + C N   G+ C C  G+
Sbjct: 4174 CNVGFQRLENALVTDSCTDIDECLLSESCATNGECQNTIGSFKCECKDGFIGDGKYDCRIDSKCEKYGGCHEDASCIVNVESSIYECQCHTEFSGDGITSCIKNNLCFVNGKSSCHENAICEQVNATYRCICPDGFQGDGYNFCNDIDECANDNTHNCSSLEKCVNSNGQYNCVCGDGAVY-VDDICVDIDECSSNQTNKCSSNAICQNKEGTYSCQCAEGF-YGDGLLCHDIDECKFGVANCS-ENALCINKPGTFACECQAGTLGNGTTCGDEDECKKPKGSPGAPECDENAVCKNKDPGYTCECQSGY 4481          

HSP 3 Score: 111.309 bits (277), Expect = 6.352e-24
Identity = 108/353 (30.59%), Postives = 149/353 (42.21%), Query Frame = 3
Query: 2577 CGKGYQ-KIGDNCIDINECDSLA-CHNNSRCINTPGSFVCYCSNNEEYLNP-----EFGCAPKIEECSFER----------------------------KIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQ----YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY---------------EYV---EDKGCI-----DIDECQASKKLCQGD---------------------NVVCQNTPGSFKCVCPVGLEYKEKG-CVDIDECQQNNT--CPEGSTCINSFGGFNCTCNKGFVFNND 3371
            C  GY+      C D+NEC + + CH  ++CINTPGS+ C C  N  Y +P     E  C  K  +C                                 +  +C  + C + A C   NG   C C  G+ Y   E C+D++EC+     C + + C+N  GSF C C  GFQ      V   C DIDEC  S+ C+ NG CQN IGS++C C +G+               +Y    ED  CI      I ECQ   +   GD                     N +C+    +++C+CP G +      C DIDEC  +NT  C     C+NS G +NC C  G V+ +D
Sbjct: 4008 CKSGYEINPSGMCQDVNECQTQSPCHILAQCINTPGSYKCQCPENF-YGDPQNKCFEDKCKTKALQCQAGEVCKLSLYGSVCEKISCNSSEILINNECLPVNQVCQNINCGNHAFCKIENGRADCFCDSGY-YGDGELCVDVDECSNGDVSCPNNSFCQNKDGSFHCACNVGFQRLENALVTDSCTDIDECLLSESCATNGECQNTIGSFKCECKDGFIGDGKYDCRIDSKCEKYGGCHEDASCIVNVESSIYECQCHTEF-SGDGITSCIKNNLCFVNGKSSCHENAICEQVNATYRCICPDGFQGDGYNFCNDIDECANDNTHNCSSLEKCVNSNGQYNCVCGDGAVYVDD 4357          

HSP 4 Score: 103.219 bits (256), Expect = 1.587e-21
Identity = 106/361 (29.36%), Postives = 162/361 (44.88%), Query Frame = 3
Query: 2370 RLTEMTTEDKEALLTEINLLKDEGHRLTFITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDN--CIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTY--DLKEGCIDINECNMNP----CLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ---GDNVVCQNTPGSFKCVCPVGL---EYKEKGCVDIDECQQN-NTC-PEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDK 3395
            +  E T  D   + T  + L  +G   T     N+  +      L+    + L   ++ +    C   +C  GY KI     C++ NEC +     C  N+ CI+T GS+ C C +  + L P   C   I EC  +    SI     C+  A+  S      C C  GF +  +    C DI+EC  NP     +  + C N+ GSF+C CP GF     + C DI+EC     C  N +C N +  + C+C NG   + +  C  I+EC  +   CQ   G + VC + P  + CVCP G    E   + C + +EC+ N ++C    S C++++G F C C  GF  N+D    + D+
Sbjct: 4856 KFAECTNNDGAYVCTCPSYLTGDGK--TTCEDQNECALETHTCNLNTSYCENLDNTVNVLAPFQC---HCFNGYVKIPGTQICLNKNECLNPVENDCVENAICIDTEGSYECRCKSGFKQL-PSGKCE-NINECEEKSDNCSI--NSVCQDKADGFS------CQCKPGFKWSDETNLNCEDIDEC-TNPTSCSSMPHSICMNNPGSFECVCPHGFSLQGGY-CNDINECLIPNSCGQNSVCANTVSGFNCSCANGARQLPNGDCELINECLENIDRCQVLNGADSVCYDLPIGYACVCPDGFVNDEVFPQVCENQNECEINIHSCQASNSECVDTYGSFKCVCKPGFSKNSDGDCVDYDE 5199          

HSP 5 Score: 97.4413 bits (241), Expect = 9.106e-20
Identity = 97/340 (28.53%), Postives = 143/340 (42.06%), Query Frame = 3
Query: 2577 CGKGYQKI-GDNCIDINECD--SLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNM-NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS------------------------------------KIC--------------SGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDEC----QASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-------QNNTCPEGSTCINSFGGFNCTCNKGFVF-NNDTKFCELDK 3395
            C  G++++    C +INEC+  S  C  NS C +    F C C    ++ +        I+EC+      S+  ++   +P       G   CVCP GF+  L+ G C DINEC + N C   + C N+   F+C C  G +   +  C  I+EC ++                                    ++C              + N  C +  GS++C C  G+    D  C+D DEC    Q++ K C  +N  C NT G F C CP G  Y    C+D+DEC         +N       CIN+ GGF C CN+GFV   N TK  ++D+
Sbjct: 4964 CKSGFKQLPSGKCENINECEEKSDNCSINSVCQDKADGFSCQCKPGFKWSDETNLNCEDIDECTNPTSCSSMPHSICMNNP-------GSFECVCPHGFS--LQGGYCNDINECLIPNSCGQNSVCANTVSGFNCSCANGARQLPNGDCELINECLENIDRCQVLNGADSVCYDLPIGYACVCPDGFVNDEVFPQVCENQNECEINIHSCQASNSECVDTYGSFKCVCKPGFSKNSDGDCVDYDECEISEQSNMKFC--NNGKCINTAGGFTCDCPEGFIYNNYDCIDVDECSLDQVSSLADNCDQVNGHCINTVGGFQCYCNRGFVMIENSTKCVDIDE 5292          

HSP 6 Score: 97.4413 bits (241), Expect = 9.338e-20
Identity = 103/317 (32.49%), Postives = 148/317 (46.69%), Query Frame = 3
Query: 2541 SKVFDITCKDVNCGKGYQKIGDNCIDINEC-DSL-ACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNM--NPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDEC--RDSKICSGNGICQNMIGSY-RCACHNGYEYVEDKGCI---------DIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEK-GCVDIDECQ-QNNTCPEGST-------CINSFGGFNCTCNKGFVFNNDTKFC-ELDKTLNVVQ 3413
            SK    TC+   C +G+Q+I  +C+D++EC D + +C NN+ C N  GS+VC C +N +    +  C  +  EC  E  +I       C   + C +++G   C C  G+ ++    C DI+EC +  +PC D A C N  GS  C C  GF     + C++ D C  RD   C     C NMIG++  C C NGYE      CI         DIDEC   K  C      C N  G++ C CP  L    K  C D +EC  + +TC   ++        +N    F C C  G+V    T+ C   ++ LN V+
Sbjct: 4643 SKDSSYTCQ---CIEGFQRINGSCVDVDECADKIDSCKNNATCNNMIGSYVCKCPDNLKLDLSKKACTDR-NECQ-ENPMI-------CGDLSKCLNTDGSYECQCVTGYKWN-GTNCEDIDECAIKTHPCHDLARCSNVPGSCKCKCQPGFTGDGIYSCIETDSCPARDDVKCPTGSYC-NMIGNFIYCNCTNGYEDSNQMECIGDVCYKKCSDIDECVTRKSACH-KFAECTNNDGAYVCTCPSYLTGDGKTTCEDQNECALETHTCNLNTSYCENLDNTVNVLAPFQCHCFNGYVKIPGTQICLNKNECLNPVE 4944          

HSP 7 Score: 95.9005 bits (237), Expect = 3.105e-19
Identity = 89/272 (32.72%), Postives = 126/272 (46.32%), Query Frame = 3
Query: 2625 ECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC-NMNPCLDKAT--CRNSYGSFDCFCPKGFQYSVHFG-CVDIDECRDS-KICSGNGI-CQNMIGSYRCACHNGYEY--VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPE-GSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQ 3413
            EC ++ C N++ C      + C+C N   ++     C   I+ECS    +        C S A C ++ G   C C  GF  +    C +INEC  ++PC D AT  C +    ++C C KGF  +   G C D +EC D+  +C      C N  GSYRC C  G+    ++   C D++EC    + C  +   C NTPGS+ C C  G +   + C DIDEC  N  C    +TC+N  G + C C  G      T  C+ D   N VQ
Sbjct: 3171 ECTNITCSNDAFCDFKDDDYGCHCKNG--FMGDGIMCK-DIDECSNNTSV--------CDSNAFCKNTIGSFSCSCKEGFEGN-GVTCTNINECYPLSPC-DNATEECFDKVPGYECRCQKGFMKNTTTGNCDDRNECADTGNLCDRVSTNCNNTFGSYRCDCKYGFRPSPIDSFVCTDVNECNLVHE-CDKNYAKCTNTPGSYYCTCDSGYQGDGRNCTDIDECSSNKVCQRPDATCVNLPGSYECRCLDG------TPGCDGDNPCNTVQ 3422          

HSP 8 Score: 93.9745 bits (232), Expect = 1.284e-18
Identity = 85/273 (31.14%), Postives = 117/273 (42.86%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDSLA--------CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLK-EGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDS-KICSGNGICQNMIGSYRCACHNGYEY-VEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQ-QNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C  G+  +G+ C D NECD  +          +   C+NT GSFVC C  N+E +N        I+ C+                        G++ C        D K E CI   +                 S+ C C +GFQ  ++  CVD+DEC D    C  N  C NMIGSY C C +  +  +  K C D +ECQ +  +C GD   C NT GS++C C  G ++    C DIDEC  + + C + + C N  G   C C  GF 
Sbjct: 4561 CKPGFNMVGNTCQDKNECDPTSPDFAAQKCLASGGGCLNTKGSFVCTC--NQEQMNENNSSCKPIDSCAM-----------------------GLDNC--------DRKVEDCISSKD----------------SSYTCQCIEGFQ-RINGSCVDVDECADKIDSCKNNATCNNMIGSYVCKCPDNLKLDLSKKACTDRNECQENPMIC-GDLSKCLNTDGSYECQCVTGYKWNGTNCEDIDECAIKTHPCHDLARCSNVPGSCKCKCQPGFT 4782          

HSP 9 Score: 93.5893 bits (231), Expect = 1.545e-18
Identity = 94/309 (30.42%), Postives = 133/309 (43.04%), Query Frame = 3
Query: 2577 CGKGYQK--IGDNCIDINECDSLA---CHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINEC--NMNPC-LDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICS-GNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGL--------------------------------------EYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFV 3359
            C  G+Q     + C DI+ECD+ A       ++C N  GS+ C C       +    C   I EC        I +   C   A C  ++G  +C C  GFT    E C DI+EC  ++N C  D+ATC NS GSF C C +G+       C +IDEC      CS  +  C N+ G + C C +GY+   +  C+D +EC+     C  DN  C NT GSF C+C  G                                       E     C++ DEC+   +C   + CI++ G F C CN+G++
Sbjct: 3437 CKHGFQNNVTTNICEDIDECDTAANDCAGRKAKCANLDGSYECECLYGYRMSSINKICE-NINEC--------IDNLHQCGENAECIDTDGGYYCQCKTGFTGHCDE-CRDIDECAFSLNNCDSDRATCENSIGSFTCRCNEGYSGDGSV-CSEIDECLLGLHNCSRSHQFCFNVNGGFECRCLSGYKSDTNGSCVDENECRFFDNGCD-DNADCINTDGSFLCICRAGYIGSGRNCDPQEGLEECGKLLCPLNALCVNSTCLCKSGYENSTNQCINTDECESQTSCDVNANCIDTHGSFICYCNEGYI 3733          

HSP 10 Score: 92.8189 bits (229), Expect = 2.852e-18
Identity = 102/344 (29.65%), Postives = 136/344 (39.53%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSN------------NEEYLNPEFGCAPKIEEC-------------SFERKIIS--------------ICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG--CIDINECNMNPCLDK--ATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICS-GNGICQNMIGSYRCAC---------------------------------HNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKE--KGCVDIDECQQN-NTCPEGSTCINSFGGFNCTCNKGFV 3359
            +C  G+   G  C DI+EC  ++  C +N+ C NT GSF C C              NE Y  P   C    EEC              F +   +              +CD V+     NC+++ G   C C  GF     +   C D+NECN+    DK  A C N+ GS+ C C  G+Q      C DIDEC  +K+C   +  C N+ GSY C C                                 H     V    C DIDEC  +   C G    C N  GS++C C  G       K C +I+EC  N + C E + CI++ GG+ C C  GF 
Sbjct: 3193 HCKNGFMGDGIMCKDIDECSNNTSVCDSNAFCKNTIGSFSCSCKEGFEGNGVTCTNINECY--PLSPCDNATEECFDKVPGYECRCQKGFMKNTTTGNCDDRNECADTGNLCDRVS----TNCNNTFGSYRCDCKYGFRPSPIDSFVCTDVNECNLVHECDKNYAKCTNTPGSYYCTCDSGYQGDGR-NCTDIDECSSNKVCQRPDATCVNLPGSYECRCLDGTPGCDGDNPCNTVQCTKPNEICYLGQCFCKHGFQNNVTTNICEDIDECDTAANDCAGRKAKCANLDGSYECECLYGYRMSSINKICENINECIDNLHQCGENAECIDTDGGYYCQCKTGFT 3529          

HSP 11 Score: 92.0485 bits (227), Expect = 4.801e-18
Identity = 84/274 (30.66%), Postives = 120/274 (43.80%), Query Frame = 3
Query: 2568 DVNCGKGYQKIGDNCIDINECDS--LACHNNSRCINTPGSFVCYCSNNEEYLNPEF--GCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECN-MNPCLDKATCRNSYGS--FDCFCPKGFQYSVHFGCVDIDEC--RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQN--NTCPEGSTCINSFGGFNCTCNKGF 3356
            D  C  GY   G+ C+D++EC +  ++C NNS C N  GSF C C+   + L           I+EC             +C +   C ++ G   C C  GF  D K  C   ++C     C + A+C  +  S  ++C C   F       C+  + C       C  N IC+ +  +YRC C +G++      C DIDEC             C N+ G + CVC  G  Y +  CVDIDEC  N  N C   + C N  G ++C C +GF
Sbjct: 4130 DCFCDSGYYGDGELCVDVDECSNGDVSCPNNSFCQNKDGSFHCACNVGFQRLENALVTDSCTDIDECLLSE---------SCATNGECQNTIGSFKCECKDGFIGDGKYDCRIDSKCEKYGGCHEDASCIVNVESSIYECQCHTEFSGDGITSCIKNNLCFVNGKSSCHENAICEQVNATYRCICPDGFQGDGYNFCNDIDECANDNTHNCSSLEKCVNSNGQYNCVCGDGAVYVDDICVDIDECSSNQTNKCSSNAICQNKEGTYSCQCAEGF 4394          

HSP 12 Score: 87.0409 bits (214), Expect = 1.568e-16
Identity = 102/369 (27.64%), Postives = 133/369 (36.04%), Query Frame = 3
Query: 2577 CGKGYQKIGDNCIDINECDS-LACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPK---IEECSFERKII------SICDAVT-----------------CRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFG----CVDIDECRDS---------------------------------------KICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCV-----------------------------------CPVGLEYKEKG-CVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGF 3356
            C  GY+   + CI+ +EC+S  +C  N+ CI+T GSF+CYC  NE Y+     C  K    + C  E           IC   +                 C   A CS S+G   C C  G +       CIDI+ECN N   C + + C N +GS+DC CP  +      G     V + +C  S                                        IC  N  C+  +  Y C C  GYE V  K C DIDEC      C   N  C NT GSF C                                    C  G E    G C D++ECQ  + C   + CIN+ G + C C + F
Sbjct: 3688 CKSGYENSTNQCINTDECESQTSCDVNANCIDTHGSFICYC--NEGYIGDGINCFKKDTDTDACQIENYCTDGDCYDGICHCPSGFVFKSNKCFDERCENVCPKNAICSISSGSPVCSCSVGTSLSSDSSQCIDIDECNENVDDCQENSICFNRFGSYDCKCPPNYLDVFKDGKVCMAVPLSQCNQSCPTGQYCNNGSCSCLMGLNSQTDDDGNLNCSKNSASCLPGICPLNANCEERLFGYICKCKPGYEGVGIKSCRDIDECGGKINNCT-INEDCSNTEGSFTCSCKRGYSRNSSSGLCETNNVCNCGSHGICGPNYICQCKSGYEINPSGMCQDVNECQTQSPCHILAQCINTPGSYKCQCPENF 4053          

HSP 13 Score: 75.8702 bits (185), Expect = 3.615e-13
Identity = 97/335 (28.96%), Postives = 141/335 (42.09%), Query Frame = 3
Query: 2541 SKVFDITCKDVNCGKGYQKIGDNCIDINECDSLACHNNSR----CINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGF-----TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVED-----KGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDTKFC----ELDKTLNVVQPKADLV--FGIDTSESMKNYMDIVKD 3485
            + +   TC+   C +GY   G  C +I+EC  L  HN SR    C N  G F C C +   Y +   G      EC F            C   A+C +++G   C+C  G+       D +EG   + EC    C   A C NS     C C  G++ S +  C++ DEC     C  N  C +  GS+ C C+ GY  + D     K   D D CQ       GD   C +      C CP G  +K   C D + C+  N CP+ + C  S G   C+C+ G   ++D+  C    E ++ ++  Q  +     FG    +   NY+D+ KD
Sbjct: 3557 NSIGSFTCR---CNEGYSGDGSVCSEIDEC-LLGLHNCSRSHQFCFNVNGGFECRCLSG--YKSDTNGSCVDENECRFFDN--------GCDDNADCINTDGSFLCICRAGYIGSGRNCDPQEG---LEECGKLLCPLNALCVNST----CLCKSGYENSTN-QCINTDECESQTSCDVNANCIDTHGSFICYCNEGY--IGDGINCFKKDTDTDACQIENYCTDGD---CYDG----ICHCPSGFVFKSNKCFD-ERCE--NVCPKNAICSISSGSPVCSCSVGTSLSSDSSQCIDIDECNENVDDCQENSICFNRFGSYDCKCPPNYLDVFKD 3857          

HSP 14 Score: 75.485 bits (184), Expect = 4.205e-13
Identity = 87/263 (33.08%), Postives = 119/263 (45.25%), Query Frame = 3
Query: 2574 NCGKGYQKIGDNCIDINEC-----DSLA--CHN-NSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECNMNP--CLDKAT--CRNSYGSFDCFCPKGFQYS---VHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTC 3311
            +C +G+     +CID++EC      SLA  C   N  CINT G F CYC+     +     C   I+EC F    +   D +       C++  G   C+C  G  YDL  G C DINEC+ N   C  +A   C N+ GSF C C  G++ S    +  C  ++EC    I C  N  C +    Y C C +GY+  +   C +I+ECQ         +  C +T GS+KC C  G  YK  G V        N+C  G  C
Sbjct: 5226 DCPEGFIYNNYDCIDVDECSLDQVSSLADNCDQVNGHCINTVGGFQCYCNRGFVMIENSTKCV-DIDECQFPNSCL---DGL-------CNNLPGSFECLCNIG--YDLVNGKCTDINECSNNSQICGPQAAGICINAAGSFQCKCNVGYENSGGKSYNPCQSVNECVKLNITCPSNAQCVDRSNGYECICKDGYQEDQIGICRNINECQIEDSCHYLAD--CLDTDGSYKCTCKYG--YKGDGIVSCKAICGPNSCLPGQLC 5471          

HSP 15 Score: 72.4034 bits (176), Expect = 4.233e-12
Identity = 90/329 (27.36%), Postives = 123/329 (37.39%), Query Frame = 3
Query: 2577 CGKGYQKIGDN-CIDINECDSLACHNNS---RCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMN--PCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKI------CSGNGICQNMIGSYRCACHNGYE-----------------------------------------YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPE---------GSTCINSFGGFNCTCNKGFVFNNDTK 3377
            C  G+Q  G N C DI+EC +   HN S   +C+N+ G + C C +   Y++        I+ECS  +          C S A C +  G   C C  GF Y     C DI+EC      C + A C N  G+F C C  G        C D DEC+  K       C  N +C+N    Y C C +GY+                                          +E+  CI   EC+     C  +   C      FKC C  G       C D +EC    T P+         G  C+N+ G F CTCN+  +  N++ 
Sbjct: 4305 CPDGFQGDGYNFCNDIDECANDNTHNCSSLEKCVNSNGQYNCVCGDGAVYVD---DICVDIDECSSNQ-------TNKCSSNAICQNKEGTYSCQCAEGF-YGDGLLCHDIDECKFGVANCSENALCINKPGTFACECQAG-TLGNGTTCGDEDECKKPKGSPGAPECDENAVCKNKDPGYTCECQSGYKGTGWFCIKQTPCDVPNACKANQTCTPSNENSLAICECKPDFKLENDTCIPKTECENKTDNCDRETSNCVQLDPGFKCECKPGFNMVGNTCQDKNECDP--TSPDFAAQKCLASGGGCLNTKGSFVCTCNQEQMNENNSS 4619          

HSP 16 Score: 67.781 bits (164), Expect = 8.770e-11
Identity = 54/160 (33.75%), Postives = 75/160 (46.88%), Query Frame = 3
Query: 2919 CLDKATCRNSY--GSFDCFCPKGFQYSVHFGCVDIDEC-RDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDID-----------ECQQNNTCPEGSTCIN-SFGGFNCTCNKG 3353
            C +   C NS   G  +C C KG++      C DIDEC R S +CS NG C N +G+Y C C  G+       C D +EC++    C      C NT GS+KC+C  G E     C  I               Q+ +C + +TC++ + G F C C +G
Sbjct:  499 CHEYGECFNSTASGESECRCKKGYEGRAD-NCTDIDECSRGSYVCSDNGDCVNQLGTYNCLCKTGF-VGNGLNCTDFNECESKTANCSA-YATCYNTFGSYKCICQNGYEGNGSICSQIPIIAVNPDQTNCSFHQDASCDQLATCVDHADGSFTCDCTEG 655          

HSP 17 Score: 67.0106 bits (162), Expect = 1.605e-10
Identity = 63/218 (28.90%), Postives = 96/218 (44.04%), Query Frame = 3
Query: 3009 VDIDECRDS-KICSGNGICQNMIGSYRCACHNGYE-------YVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVG-LEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFNNDTKFCELDKTLNVVQPKADLVF-GIDTSESMKNYMDIVKDMLKN-IITDYKLKSLAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHT 3626
            +D +EC  +   C  +  C N+   +RC C  G++       Y   + CID DEC   K     D   C NTPGSF C C VG ++     C DI+EC  N++ C   + CIN++G + C C  GF  + +   CE             L F G       +    +V+D  +N +  +    +  +I   +   TG NS V   + KE    ++N+T
Sbjct: 2650 IDFNECSTNFHDCDLHANCTNLDSGFRCDCIKGWQGLGGNEMYSNGRSCIDDDECALLKCPSTSD---CTNTPGSFICNCHVGFVKAAPTVCNDINECSLNSSICDANADCINTYGSYICNCKPGFRGSGNPGDCEAVAICGCWGDPHCLSFDGNWLHYQGRCKYTLVRDECRNGLPIESSTANFEVIMKNWDQNTGTNSMVS--WAKEITVKIMNYT 2862          

HSP 18 Score: 62.3882 bits (150), Expect = 4.771e-9
Identity = 61/228 (26.75%), Postives = 91/228 (39.91%), Query Frame = 3
Query: 2799 CRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGY------EYVEDKGCIDIDECQASK-------------------------------KLCQ----GDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNN-TCPEGSTCINSFGGFNCTCNKGF 3356
            C    +CS++ G   C C RG++ +   G  + N  N+  C     C  +Y    C C  G++ +    C D++EC+    C     C N  GSY+C C   +      +  EDK      +CQA +                               ++CQ    G++  C+   G   C C  G     + CVD+DEC   + +CP  S C N  G F+C CN GF
Sbjct: 3956 CTINEDCSNTEGSFTCSCKRGYSRNSSSGLCETN--NVCNCGSHGICGPNY---ICQCKSGYEINPSGMCQDVNECQTQSPCHILAQCINTPGSYKCQCPENFYGDPQNKCFEDKCKTKALQCQAGEVCKLSLYGSVCEKISCNSSEILINNECLPVNQVCQNINCGNHAFCKIENGRADCFCDSGYYGDGELCVDVDECSNGDVSCPNNSFCQNKDGSFHCACNVGF 4178          

HSP 19 Score: 62.003 bits (149), Expect = 5.366e-9
Identity = 48/126 (38.10%), Postives = 57/126 (45.24%), Query Frame = 3
Query: 2889 IDINEC--NMNPCLDKATCRNSYGSFDCFCPKGFQ-------YSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLE 3239
            ID NEC  N + C   A C N    F C C KG+Q       YS    C+D DEC   K C     C N  GS+ C CH G+       C DI+EC  +  +C   N  C NT GS+ C C  G  
Sbjct: 2650 IDFNECSTNFHDCDLHANCTNLDSGFRCDCIKGWQGLGGNEMYSNGRSCIDDDECALLK-CPSTSDCTNTPGSFICNCHVGFVKAAPTVCNDINECSLNSSICDA-NADCINTYGSYICNCKPGFR 2773          

HSP 20 Score: 59.6918 bits (143), Expect = 2.623e-8
Identity = 36/98 (36.73%), Postives = 46/98 (46.94%), Query Frame = 3
Query: 3075 GSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNT-CPEGSTCINSFGGFNCTCNKGFVFN 3365
            G   C C  GYE   D  C DIDEC     +C  DN  C N  G++ C+C  G       C D +EC+     C   +TC N+FG + C C  G+  N
Sbjct:  512 GESECRCKKGYEGRADN-CTDIDECSRGSYVCS-DNGDCVNQLGTYNCLCKTGFVGNGLNCTDFNECESKTANCSAYATCYNTFGSYKCICQNGYEGN 607          

HSP 21 Score: 56.9954 bits (136), Expect = 1.983e-7
Identity = 41/118 (34.75%), Postives = 55/118 (46.61%), Query Frame = 3
Query: 2799 CRSPANCSSSNGVEFCVCPRGF-------TYDLKEGCIDINECNMNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECR-DSKICSGNGICQNMIGSYRCACHNGYEYVEDKG 3128
            C   ANC++ +    C C +G+        Y     CID +EC +  C   + C N+ GSF C C  GF  +    C DI+EC  +S IC  N  C N  GSY C C  G+    + G
Sbjct: 2662 CDLHANCTNLDSGFRCDCIKGWQGLGGNEMYSNGRSCIDDDECALLKCPSTSDCTNTPGSFICNCHVGFVKAAPTVCNDINECSLNSSICDANADCINTYGSYICNCKPGFRGSGNPG 2779          
BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Match: SMESG000032551.1 (SMESG000032551.1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.310e-16
Identity = 81/281 (28.83%), Postives = 123/281 (43.77%), Query Frame = 3
Query: 2436 EGHRLT-FITINNDVDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDINEC--DSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKIISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEG-CIDINECN--MNPCLDKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMIGSYRCACHNGYEYVEDKGCIDIDECQASKKLCQ------GDNVVCQNTPGSFKC---VCPVG 3233
            EG+R    I I  D+D     Y+L     KE+   + K    TC +  C  G  KI + C D++EC  +   C +  RC N  G + C           E  C    E                         +N  + C C  GF  +L  G C D++EC+  +  C +K  C+N  G ++C C  G+++  +  C+DIDEC   K   G+  C+N  G ++C C +GY+   +  C DI+EC  +  +C+       + V C N  GS+KC   +CP G
Sbjct:  117 EGYRWNPLINICEDIDECTGPYRLQCEPFKEIC--VRKPGLSTC-ETQCSGGTIKIANICYDVDECAENKNNCTDTQRCNNYFGYYTC----------SEIKCEGYEE------------------------LNNATKECHCKSGFKRNLYTGKCEDVDECDSLIPVCGNKGRCKNEIGRYNCQCNPGYEFK-NATCLDIDECVSLKDICGHLKCENTPGFFKCVCKSGYKMSNENVCEDINECTDTPGICRIERLSLNNRVECFNLVGSYKCLESICPSG 359          

HSP 2 Score: 77.7962 bits (190), Expect = 2.981e-14
Identity = 93/314 (29.62%), Postives = 129/314 (41.08%), Query Frame = 3
Query: 2568 DVNCGKGYQKIGD--NCIDINECDSLA--CHNNSRCINTPGSFVC----YCSNNEEYLNPEFGCAPKIEECSFERKIIS---ICDAV----TCRSPANCSSSNGVEFC-----VCPRGFTYD-LKEGCIDINECNMNPCLD----KATCRNSYGSFDCFCP-KGFQYSVHFGCVDIDECRDSKI-CSGNGICQNMIGSYRCA------------------CHNGYEYVEDKG-CIDIDECQASKKLCQGDNVVCQNTPGSFKCVCPVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNND 3371
            +V C  GYQ + +   C DINEC   A  CH N  C N PGS+ C    YC    E+ N E      + EC+   K      +C+ V     CR P     +N   +       C  G+ ++ L   C DI+EC     L     K  C    G   C     G    +   C D+DEC ++K  C+    C N  G Y C+                  C +G++     G C D+DEC +   +C G+   C+N  G + C C  G E+K   C+DIDEC           C N+ G F C C  G+  +N+
Sbjct:    5 NVTCESGYQFVFEEKKCKDINECSQKADICHINEYCRNLPGSYKCIVKDYCGQGYEF-NIETQTCIDLNECAQSLKYCDSGMVCENVIGSFNCRPPC---PTNKPHYWNSGCHQCKEGYRWNPLINICEDIDECTGPYRLQCEPFKEICVRKPGLSTCETQCSGGTIKIANICYDVDECAENKNNCTDTQRCNNYFGYYTCSEIKCEGYEELNNATKECHCKSGFKRNLYTGKCEDVDECDSLIPVC-GNKGRCKNEIGRYNCQCNPGYEFKNATCLDIDECVSLKDICGHLKCENTPGFFKCVCKSGYKMSNE 313          
The following BLAST results are available for this feature:
BLAST of Calcium binding egf domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FBN31.182e-3736.86fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794][more]
FBN22.707e-3439.85fibrillin 2 [Source:HGNC Symbol;Acc:HGNC:3604][more]
FBN22.707e-3439.85fibrillin 2 [Source:HGNC Symbol;Acc:HGNC:3604][more]
FBN31.350e-3337.82fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794][more]
FBN31.350e-3337.82fibrillin 3 [Source:HGNC Symbol;Acc:HGNC:18794][more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fbn-15.956e-2630.15FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7... [more]
fbn-11.124e-2530.15FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7... [more]
fbn-11.202e-2530.15FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7... [more]
fbn-11.435e-2530.15FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7... [more]
fbn-11.544e-2530.15FiBrilliN homolog [Source:UniProtKB/TrEMBL;Acc:I7... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dpy1.929e-3437.93gene:FBgn0053196 transcript:FBtr0305142[more]
dpy2.266e-3437.93gene:FBgn0053196 transcript:FBtr0305141[more]
dpy2.608e-3437.93gene:FBgn0053196 transcript:FBtr0332352[more]
dpy5.263e-3437.93gene:FBgn0053196 transcript:FBtr0332344[more]
dpy6.448e-3437.93gene:FBgn0053196 transcript:FBtr0332349[more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fbn2b1.106e-3439.64fibrillin 2b [Source:ZFIN;Acc:ZDB-GENE-090112-3][more]
fbn2a1.405e-3339.02fibrillin 2a [Source:ZFIN;Acc:ZDB-GENE-090112-4][more]
hmcn29.403e-3334.15hemicentin 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9765... [more]
ltbp11.943e-3138.95latent transforming growth factor beta binding pro... [more]
hmcn12.887e-3134.15hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322][more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
paqr88.059e-3738.41progestin and adipoQ receptor family member VIII [... [more]
FBN31.758e-3538.91fibrillin 3 [Source:NCBI gene;Acc:100151721][more]
FBN32.013e-3538.91fibrillin 3 [Source:NCBI gene;Acc:100151721][more]
FBN32.050e-3538.91fibrillin 3 [Source:NCBI gene;Acc:100151721][more]
FBN32.241e-3538.91fibrillin 3 [Source:NCBI gene;Acc:100151721][more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Hmcn19.140e-3737.17hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047][more]
Fbn22.807e-3439.47fibrillin 2 [Source:MGI Symbol;Acc:MGI:95490][more]
Ltbp17.119e-3435.41latent transforming growth factor beta binding pro... [more]
Ltbp17.121e-3435.41latent transforming growth factor beta binding pro... [more]
Ltbp17.249e-3435.41latent transforming growth factor beta binding pro... [more]
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BLAST of Calcium binding egf domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P35556|FBN2_HUMAN1.300e-3339.85Fibrillin-2 OS=Homo sapiens OX=9606 GN=FBN2 PE=1 S... [more]
sp|Q61555|FBN2_MOUSE1.963e-3339.47Fibrillin-2 OS=Mus musculus OX=10090 GN=Fbn2 PE=1 ... [more]
sp|Q8CG19|LTBP1_MOUSE2.923e-3335.41Latent-transforming growth factor beta-binding pro... [more]
sp|Q75N90|FBN3_HUMAN6.485e-3337.82Fibrillin-3 OS=Homo sapiens OX=9606 GN=FBN3 PE=2 S... [more]
sp|Q00918|LTBP1_RAT1.241e-3234.75Latent-transforming growth factor beta-binding pro... [more]
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BLAST of Calcium binding egf domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267ED601.129e-14730.52Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F2934.643e-14629.76Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8G2Y13.614e-14529.76Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I5M91.303e-14330.21Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GFI71.798e-14331.47Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fbn2a1.478e-3237.74fibrillin 2a [Source:ZFIN;Acc:ZDB-GENE-090112-4][more]
fbn2b5.712e-3035.55fibrillin 2b [Source:ZFIN;Acc:ZDB-GENE-090112-3][more]
ENSAMXT00000047922.16.562e-2930.87pep primary_assembly:Astyanax_mexicanus-2.0:3:7287... [more]
ENSAMXT00000035254.16.562e-2930.87pep primary_assembly:Astyanax_mexicanus-2.0:3:7287... [more]
ENSAMXT00000010372.28.666e-2930.87pep primary_assembly:Astyanax_mexicanus-2.0:3:7287... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002704.15.194e-3939.71pep scaffold:Pmarinus_7.0:GL476354:513769:588114:-... [more]
ENSPMAT00000006555.13.339e-3035.45pep scaffold:Pmarinus_7.0:GL476370:200676:210822:-... [more]
ENSPMAT00000000017.11.286e-2934.44pep scaffold:Pmarinus_7.0:GL477746:39241:65692:1 g... [more]
ENSPMAT00000005398.12.726e-2734.50pep scaffold:Pmarinus_7.0:GL477786:13802:46188:-1 ... [more]
ENSPMAT00000008854.16.347e-2635.55pep scaffold:Pmarinus_7.0:GL476328:1008604:1022137... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO351099.451e-3337.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO277424.035e-3234.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO297641.341e-3032.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494102.089e-3036.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387874.645e-3035.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FBN18.997e-3436.57fibrillin 1 [Source:NCBI gene;Acc:101171649][more]
fbln21.528e-2933.45fibulin 2 [Source:ZFIN;Acc:ZDB-GENE-060711-1][more]
ENSORLT00000033645.12.031e-2936.03pep primary_assembly:ASM223467v1:15:6710262:671709... [more]
ENSORLT00000027703.14.037e-2834.34latent transforming growth factor beta binding pro... [more]
ENSORLT00000000411.26.263e-2835.38latent-transforming growth factor beta-binding pro... [more]
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BLAST of Calcium binding egf domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034758.10.000e+099.94SMESG000034758.1[more]
SMESG000021248.11.448e-2933.98SMESG000021248.1[more]
SMESG000032548.11.242e-2532.77SMESG000032548.1[more]
SMESG000076582.11.712e-2532.74SMESG000076582.1[more]
SMESG000032551.11.310e-1628.83SMESG000032551.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008893 ID=SMED30008893|Name=Calcium binding egf domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=5111bp
AATTCACCGACATGGCAAAACATATCAAAAACTAAATACCGAATAATCAA
CAAATCTGAATATTAATTAAAATAATTTTAACAGACATGTTTTTTGACAG
AAAACCACCAGCTATTTGAAAAAGCTTAGGAAACTACAATAAAATCACAT
GTTAATTGAAAATTTTGTGTATAAATATTGGGCATTGTTGGAAGATCTTT
AGAAAACGTGTTAATTTCAACTAAATGATCATAAATAATTGTATATGGTT
ACTGATTACGGTAACATTTACCGGAATTTTACAAACGGAATCCAGGTTAT
ATTATGATTTGAAAAGATTTCCCCGAATTGCTGATACTCAATACTTTATG
AGTAGTTCTGGAGAGATTCGAATAAAAAGACAAAGCGACGTAACTTCTTC
TCTTGAAGTTGTAGATCCATATTGTACTATTGGTGATCTGGATGGAAAAG
TCTGTAATGAATACTTGCCAATGAATAAAGATAAATCATGCCCATCTGAT
TCGATTCCAAAGAAAAATAGATGTGTCAGATTTCGCAATCCAAGATGTCC
AAGCCGGTATACTAATGTGGACGGAAAATGCATATTAGATAGGGGTTGTG
ATTTAAAGGAATATGACCAAATCGGAAGAAACTGTTACGAGAAATGCCAG
TGGAGTCACAGAAGGTATTACAGTAATTTATGCATCAGTGAAAAGCCGGG
CTGTCCCCTTCAATTCAGATGGAATGGAACCGATCATTGCATTTTGAGCG
AATGTCCAACGCTGTTAGACCAGACTGAAGACGGACTCTACTGTCAAACT
CGATGCCCTCCTTATTTTGAAAAAATTAACTCTTCCACTTGTGAAAGCAA
TCGAACTATTTGTCCAATTGGATATGATAAAGAAGGAACTTATTGCATCC
AAAAAACATGTGAAAGAGGTCTACTATTGAACGGATTCTGCTTAGATACC
ATTTGCAAAACTAATTACGAATTCGATGGGAAGGTCTGCAAAAAATGTTT
AACAAGTACACACTCATATTGCTCAGCAATGGATTGGACTTGTCCTATGG
GTCTCATTGAAATTGGAAGTGGTCCACATATGCAATGCGTCATGATCTGT
CCGAAAAGATATGCACAATTTGGAAAGTCTTGTTACGATATATCAGTTAC
TGGTTGCCCTGAAGGATATTTACATTTCGAAGATAATACGTGTATTGAAG
AGTCGAAATGTCCAGATGGATTCGAAATTTTTGGCACTGAATGCGTTCGA
AAACTAACATATGTTGGATTTATTATAAATTCGAAAGAAGTCCAGTTGTG
TCCCACTGATTACATTGAATATCAAGGAAAATGTTTCAAGCTGACCGATA
ACGCTTGTCCAGATGGAACATTGGCCATCGAAGATAGTCATTATTGCGTT
TCAACTTGTCCTCAAGGATTCAATAATGTCCAGAAAGCTTGCATTGTTTC
AAGTGAATGTAAGAGTAATGAATTTTCCTTCATGGGAAAATGTCATGAAA
AATGTCCATTCGGAACCAAAGATTCGAATAATACAAGAAGTTGCAAAGTG
GTGCCAAACTGTGAAAAAAATCAAGTTCTAGACCTACTAAGTTTCCGTTG
TGTCGACAGATTAGCATGCCAGAAAAACTACCTTGTTAGTCCTGATGGAA
AATTCTGTCACAGGAAAGACCAATGCAATGGGCAAGGCTATATATTAGAC
CAATATTGCATTAGCTCATGCCCCGTCGATTATGACACCGACGCTCAGTT
CTGTAAAAGAAGACCGGCTGAATGTCCTAGTCTTAGTTTATCCAATGGAA
CATGTGTATCGGAATGTCCAGAAGGTCATGTTCCGAAAAATAGAATATGC
GTTTCAGTAAAAATTGACAAAAATTCAGTAGTTTGCGGAGTTAACAAAGA
ACTTCAAGACTGTGCTAGTTCCAATGGCGGTGAATGCAATACAAAGTGTG
CTTGCAAACCCGGATTCGTTGAAGCGAAAGGCGAAGATTATTGCATTCCG
AAAGAATCTAAATGTCCTTCAAAACATATGCTGGATATGGTGTTCATCAT
TGATGGTTCCGAGTACGTCACTGAAAGCATGTTTAATGAAGTCAAATTCT
TCTTAATGGAAACCATAACCCAAATTTATTCATCTGGCATCAATGTTCGT
GTTGGGATTGCAGTTTATGGGCCGGAAAATCCTACTATTGTTTTATTGGA
TAAAACAGAAAAATTATGCGATTGCATGAAAATCGTCACTCAAATAAAAT
ATATCCCAGGACCAAAGAAAACTACACTTATCGACGCGTTCAGCATCACG
GGAAAAATCGTCCTTTCTCAGTGTAATGGAGCAAGACTTGGATCGGCTAG
ACAGATATTTGTTGTTGGAAGATTAACGGAAATGACAACAGAAGATAAGG
AAGCATTGTTGACCGAGATAAATTTACTGAAGGATGAAGGTCATCGGTTA
ACCTTCATTACTATAAATAATGATGTGGATATTCCCCGGAGCATTTATCA
ATTAAGTGTCAGGACAACAAAAGAATTATCCCAGCATCTTTCTAAAGTTT
TTGATATTACTTGCAAAGACGTCAACTGTGGAAAAGGCTATCAAAAGATC
GGGGATAATTGCATAGACATTAACGAATGCGATTCCCTGGCTTGTCATAA
CAATTCTAGGTGTATCAATACACCAGGATCATTCGTATGTTATTGTTCGA
ACAACGAAGAATATCTGAATCCGGAGTTTGGCTGTGCACCCAAAATAGAA
GAATGCTCATTTGAGCGTAAAATCATCTCTATTTGTGACGCTGTTACTTG
TCGTTCACCAGCAAATTGTTCATCCAGCAACGGAGTAGAATTTTGCGTGT
GTCCTCGAGGATTTACATATGATCTTAAAGAAGGCTGTATCGATATCAAT
GAATGTAATATGAATCCATGTCTTGATAAAGCAACTTGCAGAAATTCATA
TGGGTCATTTGACTGTTTCTGTCCAAAAGGATTCCAGTACAGTGTGCATT
TTGGTTGTGTTGATATTGACGAATGCCGAGATAGTAAAATCTGCAGTGGC
AATGGAATTTGCCAAAACATGATCGGATCTTATCGTTGTGCTTGCCATAA
CGGTTATGAGTATGTTGAGGACAAAGGATGCATTGATATTGACGAATGTC
AGGCATCGAAAAAGTTGTGCCAAGGAGATAATGTTGTTTGTCAAAATACT
CCAGGATCCTTCAAGTGTGTTTGTCCAGTTGGTTTAGAATACAAAGAAAA
AGGATGTGTTGATATTGATGAATGTCAGCAGAACAACACATGCCCTGAAG
GATCAACATGCATCAATTCGTTCGGGGGCTTTAATTGTACGTGCAATAAG
GGATTCGTTTTCAATAATGATACAAAATTTTGTGAATTGGACAAAACATT
AAATGTGGTCCAACCGAAAGCTGATCTCGTCTTTGGAATTGATACGAGCG
AATCTATGAAAAATTATATGGATATTGTGAAAGACATGCTAAAAAATATA
ATCACCGATTACAAATTAAAGTCTTTGGCGATAATAAAAAGCTATTTTGC
CCTTACAACCGGCGGCAACTCAGAGGTGAAAGTTATCTACATAAAAGAGA
CAGATAATAACGTATTAAATCACACTGATTCAATAACTTTTATGGGAGGA
AAGTCTCTTTTATCTTCTGGCTTGAAAAGTGCGAAAGATATGTATCTACG
ACACGGGCAATACAATTCGAGTAAACTTGTGATTTTCATTACTGCAACCA
AGCCAAATTCTTATGATAAAAGTGAAATTATAGAAATAAAAGAACAAGTT
AAAGAAATCAAGAAAGATGGTCGACTGCTTATCATCTACGTTGGTGACGA
TGTTAAAAGTAATGAATGGAAGGTCATTTTCAGAGAAGAAGAAATAGAGG
AGCATGTAGTGGCGGTGTCCCAAGAATTTATGACCACAATTCACAAAGTG
ATCTATCAAAAAACAGATCGATTAATAAAATGCCCAGAAGGCTTCATTTT
CTTGGACAAAGTTTGCCAATACAAAACTACAAAGGCAATTTACTGCAAAT
GGGATGACAAATTATATGCACTCAATTCTACTTGGACCAGTTCTTGTGCG
GTGTATGCTTGTAGAGAAAACGGAGTTGTCGTACTTGAAACTAACTGTAT
GGGCTTCGACGGAGATTGTTATAAGGAAGATGAATTCTTTCCATGTACTC
TGAATGGAAATTTGAATTGGAGGTGCAAGTGTAAAATTGTCCAATACACA
GATGAAGAAACAAAGAAATCCCAACAAAGAATTGACTACATAATTATAAA
TTTCGACAAAAATGCAACAATCGAAAGCACAAAGCTGGAAGATGAGAATG
GAAAATGCGTCCTACCTTTTAAATTTGAAAACAAATGGTATTACGCTTGT
ACCGATTATAAGAGCTACAACAGATGGTGCGCCCACCACATGTACTACCA
GAGAGGATTGTACTCAAATTGTGCTCGACAAGTAACAAGTCAATGTATAT
TCGACGGTAAAATTTACAAAGAAGGTGACAAATGGAGTGTTGGCTGTAAT
GTCGTAGTTTGTGACTCTGGCAAACCTTATATTACCGCCCGATGCCAGAC
AATGAACGGAACTTGCGTGCCTCTAAACGCCGATAATTATCCATGTCAAG
TTGGTGGTGAACAATATGGATCATGCATGTGTTTGCAGGATAATTTCGGA
TACGGCGAAAATACGGCCATTAAGGACAAGATTACCGGTGAAAAAACAAA
ACTCACTGGAGGAGGTGGGTTAATGGAATTCTGCGAAGATCCTTCAAGCG
GCCAAAGATATGAAGTTGGAAAAAGATGGGAAAAGGATTGTTTTACTTTA
CAGTGTAATCCAGATGGAATTGCAAAAGTTATGGATAAAAAATGTTCATC
ATCAGATGGAAAGTGTATTGAACCCGGAAGCAATACATTTTTCACTTGTG
AAATTGGAGGCAAAGTTACACAAAATTGTCTTTGCGCCTTGGATAATGAT
GAATTAGTACTCGTCTCGAATGAAGGAGGCAAGATCGAAAGAAAAGTAGT
CGATACGAAGAAGATCGTAAATATTTAATGAAACCAATTAAACTGATTTG
ACAAATAATAA
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protein sequence of SMED30008893-orf-1

>SMED30008893-orf-1 ID=SMED30008893-orf-1|Name=SMED30008893-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1618bp
MIINNCIWLLITVTFTGILQTESRLYYDLKRFPRIADTQYFMSSSGEIRI
KRQSDVTSSLEVVDPYCTIGDLDGKVCNEYLPMNKDKSCPSDSIPKKNRC
VRFRNPRCPSRYTNVDGKCILDRGCDLKEYDQIGRNCYEKCQWSHRRYYS
NLCISEKPGCPLQFRWNGTDHCILSECPTLLDQTEDGLYCQTRCPPYFEK
INSSTCESNRTICPIGYDKEGTYCIQKTCERGLLLNGFCLDTICKTNYEF
DGKVCKKCLTSTHSYCSAMDWTCPMGLIEIGSGPHMQCVMICPKRYAQFG
KSCYDISVTGCPEGYLHFEDNTCIEESKCPDGFEIFGTECVRKLTYVGFI
INSKEVQLCPTDYIEYQGKCFKLTDNACPDGTLAIEDSHYCVSTCPQGFN
NVQKACIVSSECKSNEFSFMGKCHEKCPFGTKDSNNTRSCKVVPNCEKNQ
VLDLLSFRCVDRLACQKNYLVSPDGKFCHRKDQCNGQGYILDQYCISSCP
VDYDTDAQFCKRRPAECPSLSLSNGTCVSECPEGHVPKNRICVSVKIDKN
SVVCGVNKELQDCASSNGGECNTKCACKPGFVEAKGEDYCIPKESKCPSK
HMLDMVFIIDGSEYVTESMFNEVKFFLMETITQIYSSGINVRVGIAVYGP
ENPTIVLLDKTEKLCDCMKIVTQIKYIPGPKKTTLIDAFSITGKIVLSQC
NGARLGSARQIFVVGRLTEMTTEDKEALLTEINLLKDEGHRLTFITINND
VDIPRSIYQLSVRTTKELSQHLSKVFDITCKDVNCGKGYQKIGDNCIDIN
ECDSLACHNNSRCINTPGSFVCYCSNNEEYLNPEFGCAPKIEECSFERKI
ISICDAVTCRSPANCSSSNGVEFCVCPRGFTYDLKEGCIDINECNMNPCL
DKATCRNSYGSFDCFCPKGFQYSVHFGCVDIDECRDSKICSGNGICQNMI
GSYRCACHNGYEYVEDKGCIDIDECQASKKLCQGDNVVCQNTPGSFKCVC
PVGLEYKEKGCVDIDECQQNNTCPEGSTCINSFGGFNCTCNKGFVFNNDT
KFCELDKTLNVVQPKADLVFGIDTSESMKNYMDIVKDMLKNIITDYKLKS
LAIIKSYFALTTGGNSEVKVIYIKETDNNVLNHTDSITFMGGKSLLSSGL
KSAKDMYLRHGQYNSSKLVIFITATKPNSYDKSEIIEIKEQVKEIKKDGR
LLIIYVGDDVKSNEWKVIFREEEIEEHVVAVSQEFMTTIHKVIYQKTDRL
IKCPEGFIFLDKVCQYKTTKAIYCKWDDKLYALNSTWTSSCAVYACRENG
VVVLETNCMGFDGDCYKEDEFFPCTLNGNLNWRCKCKIVQYTDEETKKSQ
QRIDYIIINFDKNATIESTKLEDENGKCVLPFKFENKWYYACTDYKSYNR
WCAHHMYYQRGLYSNCARQVTSQCIFDGKIYKEGDKWSVGCNVVVCDSGK
PYITARCQTMNGTCVPLNADNYPCQVGGEQYGSCMCLQDNFGYGENTAIK
DKITGEKTKLTGGGGLMEFCEDPSSGQRYEVGKRWEKDCFTLQCNPDGIA
KVMDKKCSSSDGKCIEPGSNTFFTCEIGGKVTQNCLCALDNDELVLVSNE
GGKIERKVVDTKKIVNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0001527microfibril
GO:0005576extracellular region
GO:0005578proteinaceous extracellular matrix
GO:0005615extracellular space
GO:0031012extracellular matrix
GO:0043234protein complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002089reproductive organ
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036943FN_type2_sf
IPR036465vWFA_dom_sf
IPR002035VWF_A
IPR000742EGF-like_dom
IPR000562FN_type2_dom
IPR001881EGF-like_Ca-bd_dom
IPR018097EGF_Ca-bd_CS
IPR013032EGF-like_CS
IPR000152EGF-type_Asp/Asn_hydroxyl_site
IPR009030Growth_fac_rcpt_cys_sf
IPR013806Kringle-like
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding
GO:0019838growth factor binding
GO:0050431transforming growth factor beta binding
GO:0050436microfibril binding
Vocabulary: biological process
TermDefinition
GO:0003281ventricular septum development
GO:0007179transforming growth factor beta receptor signaling pathway
GO:0035904aorta development
GO:0060976coronary vasculature development
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01