Protein CEPU-1

Overview
NameProtein CEPU-1
Smed IDSMED30008883
Length (bp)403
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein CEPU-1 (SMED30008883) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein CEPU-1 (SMED30008883) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein CEPU-1 (SMED30008883) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein CEPU-1 (SMED30008883) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008883

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008883SMESG000040207.1 SmedASXL_009124SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30008883SMESG000040207.1 dd_Smed_v4_11326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008883SMESG000040207.1 dd_Smed_v4_11326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30008883SMESG000040207.1 dd_Smed_v4_11326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30008883SMESG000040207.1 dd_Smed_v4_11326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008883SMESG000040207.1 dd_Smed_v6_11326_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30008883SMESG000057565.1 SMESG000040207.1 OX_Smed_1.0.00947ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Protein CEPU-1 vs. TrEMBL
Match: A0A3S1A419 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_002130 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.202e-28
Identity = 53/112 (47.32%), Postives = 76/112 (67.86%), Query Frame = 1 
Query:   52 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            M  IRS VRP   K PWGTI+ PEFGMGYQK+T YEI+Q V RL  IP    P +  +   N K  +E++++++ RL++    K PD++R V S++  ++GVV+S+AW+GYN
Sbjct:    1 MTTIRSSVRPSQPKQPWGTILKPEFGMGYQKMTEYEIEQTVSRLNTIP---PPKEQVYHRPNPKMTQEEIDAMLDRLTQNDKSKIPDSDRRVISSSYREMGVVSSYAWKGYN 109
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BLAST of Protein CEPU-1 vs. TrEMBL
Match: V4ATP2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_157955 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.298e-28
Identity = 52/113 (46.02%), Postives = 78/113 (69.03%), Query Frame = 1 
Query:   52 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGI-DNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            M  +RS VRP S+K  WGTI+ PEFG+GYQ +T YE+ Q+VDRLYY+P  RQ    P  I +NK+    + ++++ RL+     K PD++R ++S+  + +GVV+S+AW+GYN
Sbjct:    1 MSSLRSSVRPASSKQKWGTILKPEFGLGYQNVTKYELDQIVDRLYYVPTPRQ----PLHIRNNKRMTGTEFQAMMDRLTDTENVKIPDSDRRIESSIYKDMGVVSSYAWQGYN 109
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BLAST of Protein CEPU-1 vs. TrEMBL
Match: A0A194AN66 (Uncharacterized protein OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.942e-28
Identity = 53/112 (47.32%), Postives = 76/112 (67.86%), Query Frame = 1 
Query:   52 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            M  IRS VRPKS+K PWGTI+ PEFG+GYQK+T YE+ Q V+RLY IP  ++     +    KK  +++ E++  RL+K +  K PD++R V  +    +GVV+S+AW+GYN
Sbjct:    1 MSSIRSSVRPKSSKQPWGTILKPEFGLGYQKMTEYELYQTVERLYKIPSTKE---RAYERPGKKVSEDEAEAIFERLTKVNKEKIPDSDRRVSRSIYSPMGVVSSYAWKGYN 109
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BLAST of Protein CEPU-1 vs. TrEMBL
Match: A0A2C9KV31 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106080048 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 1.840e-27
Identity = 51/112 (45.54%), Postives = 77/112 (68.75%), Query Frame = 1 
Query:   52 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            M  IRS VRPKS+K  WGTI+ PEFG+GYQ++T YEI+Q V+RL  +P  +QP    +    KK  +E++++++ RL+K    K P+++R V  ++   +GVV S+AW+GYN
Sbjct:    1 MTSIRSSVRPKSSKQAWGTILKPEFGLGYQRMTEYEIEQTVNRLAVVPPAKQPY---YHRPQKKISQEEVKAMMDRLTKADKRKIPESDRRVTVSDYSTMGVVCSYAWKGYN 109
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BLAST of Protein CEPU-1 vs. TrEMBL
Match: A0A1I8F4N1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.848e-26
Identity = 51/111 (45.95%), Postives = 75/111 (67.57%), Query Frame = 1 
Query:   61 IRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGID--NKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            +RSGVR  + K PWGTI+ P FG+G+Q+LTAYEI+Q V RLY +P++RQP  +    D   K+    + + L+ +LS+++  +  DA RT + +   K+GV+ SFAWRGYN
Sbjct:  479 VRSGVRVDTEKQPWGTILLPNFGLGHQRLTAYEIQQSVQRLYVVPLQRQPSNSWQRRDCQQKRLSVAERQRLIEKLSRYNPDRVADARRTQRDSQYNKMGVLNSFAWRGYN 589
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BLAST of Protein CEPU-1 vs. Ensembl Nematostella
Match: EDO42141 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2F6])

HSP 1 Score: 51.6026 bits (122), Expect = 3.767e-9
Identity = 31/95 (32.63%), Postives = 55/95 (57.89%), Query Frame = 1 
Query:  121 EFGMGYQKLTAYEIKQVVDRLYYIP-----VRRQPD-KNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            E  M  +  T  EI Q+V+RL+         +RQP  +    ++ K+F ++D+E +V RL++ +  +  DANRT     +++ GVV ++AW+G+N
Sbjct:   69 EIKMEQRTYTGKEINQMVERLFVRDYTNRNKKRQPKIERRTKVEPKEFDEDDVEKVVERLTRNASQRATDANRT---GAMKQQGVVNTYAWKGWN 160

HSP 2 Score: 47.7506 bits (112), Expect = 8.248e-8
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:   97 PWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDME 264
            PWGTI  P F +G+QKL+A E++ VVDRL  +       K P    NKK +K ++E
Sbjct:    6 PWGTIEKPPFELGHQKLSADEVESVVDRLSQL-------KKPSSAVNKKRRKVEIE 54
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BLAST of Protein CEPU-1 vs. Planmine SMEST
Match: SMESG000040207.1 (SMESG000040207.1)

HSP 1 Score: 252.292 bits (643), Expect = 3.489e-88
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 
Query:   22 MGDNTEYRFKMPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            MGDNTEYRFKMPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN
Sbjct:    1 MGDNTEYRFKMPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 122
[Close]
BLAST of Protein CEPU-1 vs. Planmine SMEST
Match: SMESG000040207.1 (SMESG000040207.1)

HSP 1 Score: 231.491 bits (589), Expect = 5.335e-80
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1 
Query:   52 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 387
            MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN
Sbjct:    1 MPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLTAYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVGKPPDANRTVKSANLEKLGVVASFAWRGYN 112
The following BLAST results are available for this feature:
BLAST of Protein CEPU-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S1A4191.202e-2847.32Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
V4ATP21.298e-2846.02Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A194AN662.942e-2847.32Uncharacterized protein OS=Pinctada fucata OX=5042... [more]
A0A2C9KV311.840e-2745.54Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A1I8F4N12.848e-2645.95Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Protein CEPU-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO421413.767e-932.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein CEPU-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein CEPU-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000040207.13.489e-88100.00SMESG000040207.1[more]
SMESG000040207.15.335e-80100.00SMESG000040207.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008883 ID=SMED30008883|Name=Protein CEPU-1|organism=Schmidtea mediterranea sexual|type=transcript|length=403bp
TATTACTTTTCAAATTATTCAATGGGTGATAATACGGAATATAGATTTAA
AATGCCAGTGATTCGCTCGGGAGTAAGGCCAAAGTCAGCCAAATTACCTT
GGGGAACAATTGTCCATCCAGAATTTGGAATGGGTTACCAAAAATTAACT
GCTTATGAGATCAAGCAAGTCGTCGATAGATTATATTATATTCCGGTTAG
GAGGCAACCTGATAAAAACCCGTTTGGAATAGACAACAAAAAGTTTAAAA
AAGAAGACATGGAATCTTTAGTTCGCCGCCTTAGCAAGTTCAGCGTTGGA
AAGCCACCAGATGCTAACAGAACAGTAAAATCAGCAAATTTAGAAAAGCT
TGGTGTAGTCGCATCGTTTGCGTGGAGAGGATATAATTAAATTTTCGTAT
GAA
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protein sequence of SMED30008883-orf-1

>SMED30008883-orf-1 ID=SMED30008883-orf-1|Name=SMED30008883-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=130bp
YYFSNYSMGDNTEYRFKMPVIRSGVRPKSAKLPWGTIVHPEFGMGYQKLT
AYEIKQVVDRLYYIPVRRQPDKNPFGIDNKKFKKEDMESLVRRLSKFSVG
KPPDANRTVKSANLEKLGVVASFAWRGYN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head