Ubiquitin carboxyl-terminal hydrolase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008854 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Match: USP32 (ubiquitin specific peptidase 32 [Source:HGNC Symbol;Acc:HGNC:19143]) HSP 1 Score: 471.47 bits (1212), Expect = 8.367e-142 Identity = 292/838 (34.84%), Postives = 427/838 (50.95%), Query Frame = 3
Query: 1761 TILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR-DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-DTECSEINKN-------GSSPSTQENLI---------------------------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
T LT +GG L +P L D+E++P P+W ++WYGA++++ RP+ ++ DI + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S + ILL + + IK F N ++ L+ + + FE S I+ + SSPST E F Y HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D F Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L ++ +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P + D + F+
Sbjct: 208 TSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPE--LELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDR-----AFIGNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 982 HSP 2 Score: 87.4261 bits (215), Expect = 1.879e-16 Identity = 46/101 (45.54%), Postives = 59/101 (58.42%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N KWY +NDS CKE+ EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1179 IYNLYAISCHSGILGGGHYVTYAKNPNC-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1274 HSP 3 Score: 54.299 bits (129), Expect = 2.343e-6 Identity = 26/76 (34.21%), Postives = 40/76 (52.63%), Query Frame = 3
Query: 1101 NYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
+++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + + +II+ W QW
Sbjct: 2 GHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQW 77
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Match: USP32 (ubiquitin specific peptidase 32 [Source:HGNC Symbol;Acc:HGNC:19143]) HSP 1 Score: 471.855 bits (1213), Expect = 1.749e-139 Identity = 292/838 (34.84%), Postives = 427/838 (50.95%), Query Frame = 3
Query: 1761 TILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR-DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-DTECSEINKN-------GSSPSTQENLI---------------------------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
T LT +GG L +P L D+E++P P+W ++WYGA++++ RP+ ++ DI + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S + ILL + + IK F N ++ L+ + + FE S I+ + SSPST E F Y HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D F Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L ++ +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P + D + F+
Sbjct: 538 TSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPE--LELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDR-----AFIGNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 1312 HSP 2 Score: 248.054 bits (632), Expect = 1.326e-65 Identity = 147/414 (35.51%), Postives = 221/414 (53.38%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEY-SQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LSYEEA +R+T+ EL RLK AF + F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ +ES + + E+E + + D ++R+ E + ++ + W+ N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++ + E+ + D D++ IL++ + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + + +II+ W QW
Sbjct: 1 MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGNYVIREEMERMLHVVDGKVPD----TLRKCFSEGEKVNYEKFRNWLFLNKDAFTFSRWLLSGGVYV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLEVWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQW 407 HSP 3 Score: 87.4261 bits (215), Expect = 2.075e-16 Identity = 46/101 (45.54%), Postives = 59/101 (58.42%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N KWY +NDS CKE+ EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1509 IYNLYAISCHSGILGGGHYVTYAKNPNC-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1604
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Match: USP4 (ubiquitin specific peptidase 4 [Source:HGNC Symbol;Acc:HGNC:12627]) HSP 1 Score: 330.102 bits (845), Expect = 8.269e-95 Identity = 262/903 (29.01%), Postives = 406/903 (44.96%), Query Frame = 3
Query: 1815 DFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINRE-EMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQL------VTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLN---------PNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIA-EITNQDIQDKVAHI----LNNQIFHSHVYPA------------------------DQVSVKQNSP--IPIIKPNHALDCDSVNQRHP---FVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHL----SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
D+ ++P WN NWYG + ++PI R + +H + + KVE+ LL L + ++ P+ N LS FS I + + + K NI E E R+W N + D +QD L +++E +N D TWP + Q + + + + G+ GL N+GNTCF NSA+QC+SNT LT+YF++D + EIN R NP G +G +A Y ELIK +W+G + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y ELKD+NGR D VA+EAW+ H N S++VD FHGL KS + C C SV FD + L+LP+P++ ++ E+ LV P +Y V + + S +CE +SR + ++++ + +++ K P D I++ + + + +EC + +Y R + + + + + L+G P++L+V K + E Q + D+++ L ++ S + P +Q+S + S P P+ Q P F + VNS G N+ A + N LA+DW L + +EK K K+T + L CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +P+R L++++F+ N + +Y+L A++ H G++ +GHY YA NK + KWY F+DS E +I AY+LFY+R
Sbjct: 92 DYVLVPTEAWNKLLNWYGC-VEGQQPI---VRKVV-------EHGLFVKH----CKVEVYLLELKLCENSDPT-----NVLS-------------------------------CHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSNGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEIN----RDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSD-LCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSV-DGSEC----------------VTLPVYFR----ERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNS--------------YGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTT----VALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSY-NRYWRDKLDTVVEFPIRGLNMSEFV------------------CNLSA----RPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQR 875
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Match: USP11 (ubiquitin specific peptidase 11 [Source:HGNC Symbol;Acc:HGNC:12609]) HSP 1 Score: 327.02 bits (837), Expect = 1.066e-93 Identity = 231/773 (29.88%), Postives = 362/773 (46.83%), Query Frame = 3
Query: 2145 AYRASFSHRTRITDLIELLSKKLNIN-REEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPE-ELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLN---PNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSH------------------VYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVL------AQVNSNIKHCSICHWSNF-CNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDA---IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANL 4364
++ FSH I ++ ++ + +E+ R+W E +L + D + D L I++E R D TWP +L+ + S ++ G+ GL+N+GNTCF NSA+QC+SN LTEYF+ + +L E+N NP G +G +A Y +L+K W+G IVP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL D+ GR D EVAQEAWQ H N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ + L S +C +S+ + P +++ + ++ K P I ++ Y E++ + I +Y R+ +Y ++ + LFG+P++++V +D T + + + + + L+ + + P P +P A V R PF+L +Q + + NG SD + + + Y+AIDW + + ++ D A P+ L +CIE FTT E L+K+ + C CK+ Q A KKLDLW LP ILIIHLKRF + +K V +P+R+LD ++F+ QP +E++ Y+L A++ H G ++ GHY T+A NK+S +W+ F+D+ V+E +I AY+LFY+R ++
Sbjct: 167 SHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNF----RNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKK-EYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVY--------EVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLM--LFGHPLLVSVPRD---RFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDP----EPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNG-----TSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKA--PVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFV---------------------IQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDV 889
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Match: USP11 (ubiquitin specific peptidase 11 [Source:HGNC Symbol;Acc:HGNC:12609]) HSP 1 Score: 326.635 bits (836), Expect = 3.225e-93 Identity = 231/773 (29.88%), Postives = 362/773 (46.83%), Query Frame = 3
Query: 2145 AYRASFSHRTRITDLIELLSKKLNIN-REEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPE-ELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLN---PNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSH------------------VYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVL------AQVNSNIKHCSICHWSNF-CNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDA---IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANL 4364
++ FSH I ++ ++ + +E+ R+W E +L + D + D L I++E R D TWP +L+ + S ++ G+ GL+N+GNTCF NSA+QC+SN LTEYF+ + +L E+N NP G +G +A Y +L+K W+G IVP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL D+ GR D EVAQEAWQ H N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ + L S +C +S+ + P +++ + ++ K P I ++ Y E++ + I +Y R+ +Y ++ + LFG+P++++V +D T + + + + + L+ + + P P +P A V R PF+L +Q + + NG SD + + + Y+AIDW + + ++ D A P+ L +CIE FTT E L+K+ + C CK+ Q A KKLDLW LP ILIIHLKRF + +K V +P+R+LD ++F+ QP +E++ Y+L A++ H G ++ GHY T+A NK+S +W+ F+D+ V+E +I AY+LFY+R ++
Sbjct: 210 SHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNF----RNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKK-EYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVY--------EVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLM--LFGHPLLVSVPRD---RFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDP----EPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNG-----TSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKA--PVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFV---------------------IQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDV 932
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Match: cyk-3 (Ubiquitin carboxyl-terminal hydrolase cyk-3 [Source:UniProtKB/Swiss-Prot;Acc:Q8WT44]) HSP 1 Score: 254.988 bits (650), Expect = 8.716e-69 Identity = 211/778 (27.12%), Postives = 339/778 (43.57%), Query Frame = 3
Query: 2154 ASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNL--TLIDDEDKLLQDLCLR-------NFDKILVEQRNIDL-TWPEELNQLVTLKSL-------QNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDL----NRIDAKPSYRELKDSNGRHDDEVAQ-------------EAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIY-LTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRT--VTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPL-PNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDK--RWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDA-IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANL 4364
A S + +L+ L +L + + R+W+V+ EN L+DD + L L N K+L+E R WPEEL ++ K + N + S G GL N GN C+ N+ IQC++ + LT+YF+ + +L I +LR +G + Y +L++++W I P + + F ++QHD QE ++ LLD LH + + P E DSN D + +W + N S+V LF G L+S + C C+++S F+ ++ LSLPI + ++++V R IP++Y + DS + E V+ + + + + + +PN R L L F + + ST E+ + V HRKL ++ S + FG P+++ + + E+T + + + V + ++ + HS + S + + P C+ N +PF L V+ N + C C FC GCP+ P+ S + +A+DW AL+L N E+ D S P L CIE F+ E LD QC +C+K K + +W LP LIIHLKRF+FL + R K +TV +P+++ D F+ D+ D Y A+ H G L GH++ YA + E +KW L ND +EV E+E+ AYLLFYER ++
Sbjct: 456 AQVSRSMTVDELLALCKTELRLGDGDARLWQVVKENEEGNVLLDDGAQNLHQLYSSLGKTKKVNKMKLLLEVRERGTGVWPEELRASLSGKQITAASTLSSNAQLSGRPGAVGLVNYGNFCYRNAGIQCLARVSPLTQYFLDEDNLDAIKRGNLR---RGDAAETTIEYAKLLREMWAAKKKNIAPNDFNDAIRLSSDMFECSEQHDCQEFVAFLLDQLHTSMYESNKSLHPSPEESEGTDSNKLSDSSKKKEADKEEADEEKAERSWTEYEKQNESLVTQLFTGQLRSRLICRTCQSSSSVFEPFTSLSLPIGFEDVDLYQVIVVHRDGRIPRRYGFRLSR-DSKVGNLREVVAVSSGISMSHLTIQCMSSKGTLMSRSPNHRSSNLRDELPLSSFPSG--ARLYALELPESTGEDQWR-VAMHRKLQYNHEPYILGSTAGFIVSRFGLPLIVGLDE----EVTGKKLYEDVMYQMHRFMEHS----VNSSSSRAHDP-----------CEDENSGYPFTLCLVDPNYEWCGQCPALRFCRGCPIRPDESKVFIPANC-----PIAVDWLPIALYLRYNHSQEQACEDDPSVAETWSRHFAPSSLEHCIEKFSCPETLD--AAIQCDRCEKKTMRDKVMTIWKLPKYLIIHLKRFEFLREQGRMGKCKRTVNFPLKHFDPAPFV-------------------------DKPDGNTYECIALANHYGQLSCGHFIAYAKSNE-DKWLLLNDCSVREVSEEEVDKQGAYLLFYERKDV 1174 HSP 2 Score: 86.6557 bits (213), Expect = 1.137e-16 Identity = 92/407 (22.60%), Postives = 177/407 (43.49%), Query Frame = 3
Query: 105 GVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVF-GSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDL-ITSILSSVEMMTKNYISIDDLLIWINDYD-FLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSW 1316
G S + I+S E+A+ I+++E R+++AF + + +++D+FC V G+ +P EEK + FS + + A+ + + ++S+ + ++I+E ++E + W L+ K+ + +DS + Y++++ VTHL E+IELEK + + N + + P+S E++F FDEN DG IDF ELVC +SA CRGP R +I+ D++ C+++ +E + S +Y DL + +V + ++ D IW + + +++ + ++ QI I GL+P++ E I+ + +E++ +P I+ W
Sbjct: 7 GRSNAIAPIISAEDAKTYISDEEYRRIRQAFQRF-KNGCINYDEFCYHVLGGAQIP-------------------------------------EEKRRLLFSFF-------------SHGAETISFDNLLSSLVGLCRVEEV-----QSRFIEE---YHEFASWGLSPP-KLTIPLNDSY-ISFYEVMSYVTHLSVNEVIELEKVFATISDRAVCKLNEEKWKQALGGCFPDSYAERLFAVFDENRDGQIDFRELVCTLSALCRGPLPGRISQLARIW-----------DVD-----CDKLLSDEEL------SNMYKDLNVPEEHQTVTKSSNGKSALVDFGIWAQENEKYVNEYYSMALQIGHICLGLRPESRKMELQIVNE---FEKRASELPLSEWNIVASGW 327
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Match: cyk-3 (Ubiquitin carboxyl-terminal hydrolase cyk-3 [Source:UniProtKB/Swiss-Prot;Acc:Q8WT44]) HSP 1 Score: 252.677 bits (644), Expect = 4.063e-68 Identity = 211/780 (27.05%), Postives = 339/780 (43.46%), Query Frame = 3
Query: 2154 ASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNL--TLIDDEDKLLQDLCLR-------NFDKILVEQRNIDL-TWPEELNQLVTLKSL----------QNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDL----NRIDAKPSYRELKDSNGRHDDEVAQ-------------EAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIY-LTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRT--VTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPL-PNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDK--RWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANL 4364
A S + +L+ L +L + + R+W+V+ EN L+DD + L L N K+L+E R WPEEL ++ K + Q +S G GL N GN C+ N+ IQC++ + LT+YF+ + +L I +LR +G + Y +L++++W I P + + F ++QHD QE ++ LLD LH + + P E DSN D + +W + N S+V LF G L+S + C C+++S F+ ++ LSLPI + ++++V R IP++Y + DS + E V+ + + + + + +PN R L L F + + ST E+ + V HRKL ++ S + FG P+++ + + E+T + + + V + ++ + HS + S + + P C+ N +PF L V+ N + C C FC GCP+ P+ S + +A+DW AL+L N E+ D S P L CIE F+ E LD QC +C+K K + +W LP LIIHLKRF+FL + R K +TV +P+++ D F+ + D N Y A+ H G L GH++ YA + E +KW L ND +EV E+E+ AYLLFYER ++
Sbjct: 456 AQVSRSMTVDELLALCKTELRLGDGDARLWQVVKENEEGNVLLDDGAQNLHQLYSSLGKTKKVNKMKLLLEVRERGTGVWPEELRASLSGKQITAASTLSSNAQLSDSSGRPGAVGLVNYGNFCYRNAGIQCLARVSPLTQYFLDEDNLDAIKRGNLR---RGDAAETTIEYAKLLREMWAAKKKNIAPNDFNDAIRLSSDMFECSEQHDCQEFVAFLLDQLHTSMYESNKSLHPSPEESEGTDSNKLSDSSKKKEADKEEADEEKAERSWTEYEKQNESLVTQLFTGQLRSRLICRTCQSSSSVFEPFTSLSLPIGFEDVDLYQVIVVHRDGRIPRRYGFRLSR-DSKVGNLREVVAVSSGISMSHLTIQCMSSKGTLMSRSPNHRSSNLRDELPLSSFPSG--ARLYALELPESTGEDQWR-VAMHRKLQYNHEPYILGSTAGFIVSRFGLPLIVGLDE----EVTGKKLYEDVMYQMHRFMEHS----VNSSSSRAHDP-----------CEDENSGYPFTLCLVDPNYEWCGQCPALRFCRGCPIRPDESKVFIPANC-----PIAVDWLPIALYLRYNHSQEQACEDDPSVAETWSRHFAPSSLEHCIEKFSCPETLD--AAIQCDRCEKKTMRDKVMTIWKLPKYLIIHLKRFEFLREQGRMGKCKRTVNFPLKHFDPAPFVDKP----------------------DGN--TYECIALANHYGQLSCGHFIAYAKSNE-DKWLLLNDCSVREVSEEEVDKQGAYLLFYERKDV 1177 HSP 2 Score: 86.6557 bits (213), Expect = 1.101e-16 Identity = 93/408 (22.79%), Postives = 177/408 (43.38%), Query Frame = 3
Query: 105 GVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVF-GSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTS-DFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDL-ITSILSSVEMMTKNYISIDDLLIWINDYD-FLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSW 1316
G S + I+S E+A+ I+++E R+++AF + + +++D+FC V G+ +P EEK + FS + + A+ + + ++S+ + ++I+E ++E + W L+ +PL +DS + Y++++ VTHL E+IELEK + + N + + P+S E++F FDEN DG IDF ELVC +SA CRGP R +I+ D++ C+++ +E + S +Y DL + +V + ++ D IW + + +++ + ++ QI I GL+P++ E I+ + +E++ +P I+ W
Sbjct: 7 GRSNAIAPIISAEDAKTYISDEEYRRIRQAFQRF-KNGCINYDEFCYHVLGGAQIP-------------------------------------EEKRRLLFSFF-------------SHGAETISFDNLLSSLVGLCRVEEV-----QSRFIEE---YHEFASWGLSPPKLTIPL--NDSY-ISFYEVMSYVTHLSVNEVIELEKVFATISDRAVCKLNEEKWKQALGGCFPDSYAERLFAVFDENRDGQIDFRELVCTLSALCRGPLPGRISQLARIW-----------DVD-----CDKLLSDEEL------SNMYKDLNVPEEHQTVTKSSNGKSALVDFGIWAQENEKYVNEYYSMALQIGHICLGLRPESRKMELQIVNE---FEKRASELPLSEWNIVASGW 327
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Match: F07A11.4 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:I2HA92]) HSP 1 Score: 174.481 bits (441), Expect = 6.991e-44 Identity = 96/229 (41.92%), Postives = 135/229 (58.95%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLS--AIPKKYCVLFNTIDSTSSFI 3101
V +G TGL NIGNTCF N+ +Q + N L EYF+R+ + EIN TNP G +G +A + + + +W+G I P ++K + + A QF+ QHDA E LS LLD LHED+NR+ KP + +S+GRHD +V+ EAW+ HI N SI VDLFHG LKS+V C C S+ FD + + LP+P N+ L+F L P K V ++T + + F+
Sbjct: 231 VEQGYTGLRNIGNTCFMNAVLQMLVNNIELREYFLRNHYQPEINE----TNPLGSEGRLAKAFADFMHQMWSGHQKAIEPTQIKNIVAEKASQFANFAQHDAHEFLSFLLDGLHEDVNRVKKKPLTGTV-ESHGRHDLDVSNEAWKNHILRNDSIFVDLFHGQLKSHVQCPNCDRVSITFDPF--VYLPVPFPKNKQSTDLIFWPLENQTKPYKLTVSYSTEGTVADFL 452 HSP 2 Score: 80.1073 bits (196), Expect = 9.423e-15 Identity = 56/206 (27.18%), Postives = 92/206 (44.66%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-------KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVT------YALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEI 4433
L + ++ F+ E L + + C CK+ A KKL L+ LPP+LII LKRF + +Q K ++V YP+ +LD++ F+ T P N +Y+L + CH G+ GHY++ + +K +W F+DS + AYLLFY+ + N + + + P+ V+I
Sbjct: 763 LTQMLDLFSETERLKPEESWYCSTCKENVEATKKLQLYRLPPVLIIQLKRFVYTAFTYQSSMARRSKDTRSVEYPLESLDMSPFLA-------------------ETSP-HPNSTMYDLTGVVCHSGNSYFGHYISMGRLADFDSSKTKIEWRNFDDSTVARQSTSTLQTEDAYLLFYKMRDQNVTRSIFKRHYSCDPGAPEEVKI 948
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Match: F07A11.4 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:I2HA92]) HSP 1 Score: 174.866 bits (442), Expect = 8.225e-44 Identity = 96/229 (41.92%), Postives = 135/229 (58.95%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLS--AIPKKYCVLFNTIDSTSSFI 3101
V +G TGL NIGNTCF N+ +Q + N L EYF+R+ + EIN TNP G +G +A + + + +W+G I P ++K + + A QF+ QHDA E LS LLD LHED+NR+ KP + +S+GRHD +V+ EAW+ HI N SI VDLFHG LKS+V C C S+ FD + + LP+P N+ L+F L P K V ++T + + F+
Sbjct: 418 VEQGYTGLRNIGNTCFMNAVLQMLVNNIELREYFLRNHYQPEINE----TNPLGSEGRLAKAFADFMHQMWSGHQKAIEPTQIKNIVAEKASQFANFAQHDAHEFLSFLLDGLHEDVNRVKKKPLTGTV-ESHGRHDLDVSNEAWKNHILRNDSIFVDLFHGQLKSHVQCPNCDRVSITFDPF--VYLPVPFPKNKQSTDLIFWPLENQTKPYKLTVSYSTEGTVADFL 639 HSP 2 Score: 80.4925 bits (197), Expect = 8.773e-15 Identity = 56/206 (27.18%), Postives = 91/206 (44.17%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLD-------KRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVT------YALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEI 4433
L + ++ F+ E L + + C CK+ A KKL L+ LPP+LII LKRF + R K ++V YP+ +LD++ F+ T P N +Y+L + CH G+ GHY++ + +K +W F+DS + AYLLFY+ + N + + + P+ V+I
Sbjct: 910 LTQMLDLFSETERLKPEESWYCSTCKENVEATKKLQLYRLPPVLIIQLKRFVYTAFTYQSSMARRSKDTRSVEYPLESLDMSPFLA-------------------ETSP-HPNSTMYDLTGVVCHSGNSYFGHYISMGRLADFDSSKTKIEWRNFDDSTVARQSTSTLQTEDAYLLFYKMRDQNVTRSIFKRHYSCDPGAPEEVKI 1095
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Match: H34C03.2 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:Q9TYY8]) HSP 1 Score: 167.162 bits (422), Expect = 1.274e-41 Identity = 83/192 (43.23%), Postives = 118/192 (61.46%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGR-HDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
+ G GLSN+GNTCF SA QC+SN L EYF+ + + +IN NP G G +A+ GEL+K +W+G I P K K + ++A +F+G Q DA EL++ +LD LHEDLNRI KP Y E D + + + E A ++W+ + N SI+VD HG LKS + C +C+ S++FD + LSLP+P
Sbjct: 270 TTPGACGLSNLGNTCFMASAFQCLSNMPPLREYFLANTYQNDINE----DNPLGTHGHLAMAVGELMKGMWSGDYASINPRKFKSIIGQFAPRFNGYSQQDAHELMAYVLDGLHEDLNRIKKKP-YIEDSDEDAKLPEAEYAAKSWEVYKMRNDSIIVDTLHGQLKSTLICPVCEKISIKFDPFGYLSLPLP 456 HSP 2 Score: 99.7525 bits (247), Expect = 1.083e-20 Identity = 62/177 (35.03%), Postives = 87/177 (49.15%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKT--VRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEV--DEKEITMSCAYLLFYERANLNAD 4373
L + ++ FTT+E L + + C +CKK +RA K+L LW LP ILI+HLKRFQ+ +W + T V PVR LDL T++ + AIY+L A++ H GSL GHY N KW+ FNDS + S Y+L Y R L+++
Sbjct: 700 LQETLDWFTTKEQLGEQDSWYCPQCKKHERATKQLALWKLPEILILHLKRFQY--TKWSREKLTWEVVIPVRGLDL----------------------TDKVANPNHEKAIYDLIAVSRHYGSLSGGHYTAIGYNDREQKWFDFNDSSANPTYPPAEPYESSDPYILVYRRRKLDSN 852
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Match: Usp32 (gene:FBgn0036913 transcript:FBtr0332150) HSP 1 Score: 320.472 bits (820), Expect = 1.633e-88 Identity = 199/559 (35.60%), Postives = 305/559 (54.56%), Query Frame = 3
Query: 1755 NFTILTEDGGVL---SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNG----------LSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKL-LQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNR----------KASV----SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQ-ENLIKFVIYHRKLIQKA 3344
N LT +GG L +P ++ N DFE++P +W + WYG ++ + R + + + V++E+ L L I+ H T V G +S S G + ++ KR +AY A+FS + + E L ++L + E++R+W V +N ++ +ED + L++L +R+ D++L+E RN DLTWPEEL L T + Q ++S+ + G TGL N+GNTCF N+A+Q + NT L +YF R++H E+N N G +G +A+RY EL+K++WT +T + PLKL+ ++KYA QF+G QHD+QELL LLDALHEDLNR+ KP Y ELKDSNGR D VA EAW H A N SI++DLF+G LKS V C C SVRFD +S+LSLP+P++ FE+LV ++P KY N+ D S + ++S S+ PN +L+ + +++I++ LN ++++ Y+++ E S ++ S S E +K + LI A
Sbjct: 443 NVRTLTGEGGHLKRDTPLVQ-NHDFELVPKSLWKALNRWYGDNLPLPRQV--------------------IQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVN----AANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKP-YSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNS-DCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSA 974 HSP 2 Score: 184.496 bits (467), Expect = 2.277e-46 Identity = 127/404 (31.44%), Postives = 211/404 (52.23%), Query Frame = 3
Query: 309 NNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI-ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKD--FNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLIT-----SILSSVEMMTKNYISI--DDLLIWI--NDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYD 1472
T GI+F +L G+V++T+GT EK KF ++LYCN++ + I + +Y+R + SV + + F+ + WI ++ +SKWLL SD V L ++ P YQ +AGVTHL+E +I +LEK + RL N S++ +L L+ PP+P++ + +F AFDEN DG IDF EL CG+SA CRGP ER +FC KIF+ DG + + I + + +E + ++ K +K + DL+ S + +T++ +S+ +D ++W D + P L+L+ ++C IVFGL P+ E I+ WL E++ Y+I W W+ + + + DY K +A++ T + ++ + F + Q+D
Sbjct: 7 GGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVR-----NVNLAPFESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLL-SDNCVSLTSELETPT-FYQSLAGVTHLEEKDIGDLEKEFWRLK-NTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY-KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQ-YTQHTTHTCDYCKRTASQ----RTAVDEALVCDESFNTHSLEQHD 395 HSP 3 Score: 129.413 bits (324), Expect = 1.824e-29 Identity = 83/307 (27.04%), Postives = 135/307 (43.97%), Query Frame = 3
Query: 3309 FVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQN-SPIPII--KPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSD--------------------------------------------AYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
V HRK+ + +YF+++ +T LFG P+++ + H +Y A + V + SP+P + NHA DC + PF L V ++ C+IC WS+FC GC + N+D +Y + ++ +AIDW ALHL E+ ++ + ++ +P+DL C+ +FT++E L++ Y C CK + A KKL +W LPPILI+HLKRF ++ +W KS K V +P + D ++
Sbjct: 1198 LVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGT---------------------HKDLYCAVWLQVSRLLSPLPATTEQANHAADC--LGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQ--WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYL 1479 HSP 4 Score: 82.8037 bits (203), Expect = 2.535e-15 Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3
Query: 4164 DENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKE-ITMSCAYLLFYERANLNADTFLQQVLS-NVHNTVPQAVEIISDEVESDIKKYCLI 4478
D+ D Y LYA+ H G L GHY++YA N + WY +NDS C+E+ +K I S AYLLFYER L+ + +L + + NT +E+ DE E ++KK C I
Sbjct: 1589 DQFDPRYRLYAVVSHSGMLNGGHYISYASNA-TGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEV--DETEGELKKLCSI 1692
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Match: Usp32 (gene:FBgn0036913 transcript:FBtr0332149) HSP 1 Score: 320.472 bits (820), Expect = 1.689e-88 Identity = 199/559 (35.60%), Postives = 305/559 (54.56%), Query Frame = 3
Query: 1755 NFTILTEDGGVL---SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNG----------LSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKL-LQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNR----------KASV----SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQ-ENLIKFVIYHRKLIQKA 3344
N LT +GG L +P ++ N DFE++P +W + WYG ++ + R + + + V++E+ L L I+ H T V G +S S G + ++ KR +AY A+FS + + E L ++L + E++R+W V +N ++ +ED + L++L +R+ D++L+E RN DLTWPEEL L T + Q ++S+ + G TGL N+GNTCF N+A+Q + NT L +YF R++H E+N N G +G +A+RY EL+K++WT +T + PLKL+ ++KYA QF+G QHD+QELL LLDALHEDLNR+ KP Y ELKDSNGR D VA EAW H A N SI++DLF+G LKS V C C SVRFD +S+LSLP+P++ FE+LV ++P KY N+ D S + ++S S+ PN +L+ + +++I++ LN ++++ Y+++ E S ++ S S E +K + LI A
Sbjct: 443 NVRTLTGEGGHLKRDTPLVQ-NHDFELVPKSLWKALNRWYGDNLPLPRQV--------------------IQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVN----AANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKP-YSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNS-DCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSA 974 HSP 2 Score: 184.496 bits (467), Expect = 2.113e-46 Identity = 127/404 (31.44%), Postives = 211/404 (52.23%), Query Frame = 3
Query: 309 NNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI-ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKD--FNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLIT-----SILSSVEMMTKNYISI--DDLLIWI--NDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYD 1472
T GI+F +L G+V++T+GT EK KF ++LYCN++ + I + +Y+R + SV + + F+ + WI ++ +SKWLL SD V L ++ P YQ +AGVTHL+E +I +LEK + RL N S++ +L L+ PP+P++ + +F AFDEN DG IDF EL CG+SA CRGP ER +FC KIF+ DG + + I + + +E + ++ K +K + DL+ S + +T++ +S+ +D ++W D + P L+L+ ++C IVFGL P+ E I+ WL E++ Y+I W W+ + + + DY K +A++ T + ++ + F + Q+D
Sbjct: 7 GGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVR-----NVNLAPFESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLL-SDNCVSLTSELETPT-FYQSLAGVTHLEEKDIGDLEKEFWRLK-NTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY-KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQ-YTQHTTHTCDYCKRTASQ----RTAVDEALVCDESFNTHSLEQHD 395 HSP 3 Score: 133.265 bits (334), Expect = 9.955e-31 Identity = 85/308 (27.60%), Postives = 137/308 (44.48%), Query Frame = 3
Query: 3309 FVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQN-SPIPII--KPNHALDCD-SVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSD--------------------------------------------AYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
V HRK+ + +YF+++ +T LFG P+++ + H +Y A + V + SP+P + NHA DCD S+ PF L V ++ C+IC WS+FC GC + N+D +Y + ++ +AIDW ALHL E+ ++ + ++ +P+DL C+ +FT++E L++ Y C CK + A KKL +W LPPILI+HLKRF ++ +W KS K V +P + D ++
Sbjct: 1198 LVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGT---------------------HKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQ--WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYL 1482 HSP 4 Score: 82.8037 bits (203), Expect = 2.558e-15 Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3
Query: 4164 DENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKE-ITMSCAYLLFYERANLNADTFLQQVLS-NVHNTVPQAVEIISDEVESDIKKYCLI 4478
D+ D Y LYA+ H G L GHY++YA N + WY +NDS C+E+ +K I S AYLLFYER L+ + +L + + NT +E+ DE E ++KK C I
Sbjct: 1592 DQFDPRYRLYAVVSHSGMLNGGHYISYASNA-TGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEV--DETEGELKKLCSI 1695
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Match: Usp32 (gene:FBgn0036913 transcript:FBtr0332151) HSP 1 Score: 320.087 bits (819), Expect = 1.773e-88 Identity = 199/559 (35.60%), Postives = 305/559 (54.56%), Query Frame = 3
Query: 1755 NFTILTEDGGVL---SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNG----------LSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKL-LQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNR----------KASV----SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQ-ENLIKFVIYHRKLIQKA 3344
N LT +GG L +P ++ N DFE++P +W + WYG ++ + R + + + V++E+ L L I+ H T V G +S S G + ++ KR +AY A+FS + + E L ++L + E++R+W V +N ++ +ED + L++L +R+ D++L+E RN DLTWPEEL L T + Q ++S+ + G TGL N+GNTCF N+A+Q + NT L +YF R++H E+N N G +G +A+RY EL+K++WT +T + PLKL+ ++KYA QF+G QHD+QELL LLDALHEDLNR+ KP Y ELKDSNGR D VA EAW H A N SI++DLF+G LKS V C C SVRFD +S+LSLP+P++ FE+LV ++P KY N+ D S + ++S S+ PN +L+ + +++I++ LN ++++ Y+++ E S ++ S S E +K + LI A
Sbjct: 443 NVRTLTGEGGHLKRDTPLVQ-NHDFELVPKSLWKALNRWYGDNLPLPRQV--------------------IQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVN----AANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKP-YSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNS-DCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSA 974 HSP 2 Score: 184.496 bits (467), Expect = 2.115e-46 Identity = 127/404 (31.44%), Postives = 211/404 (52.23%), Query Frame = 3
Query: 309 NNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI-ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKD--FNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLIT-----SILSSVEMMTKNYISI--DDLLIWI--NDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYD 1472
T GI+F +L G+V++T+GT EK KF ++LYCN++ + I + +Y+R + SV + + F+ + WI ++ +SKWLL SD V L ++ P YQ +AGVTHL+E +I +LEK + RL N S++ +L L+ PP+P++ + +F AFDEN DG IDF EL CG+SA CRGP ER +FC KIF+ DG + + I + + +E + ++ K +K + DL+ S + +T++ +S+ +D ++W D + P L+L+ ++C IVFGL P+ E I+ WL E++ Y+I W W+ + + + DY K +A++ T + ++ + F + Q+D
Sbjct: 7 GGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVR-----NVNLAPFESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLL-SDNCVSLTSELETPT-FYQSLAGVTHLEEKDIGDLEKEFWRLK-NTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY-KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQ-YTQHTTHTCDYCKRTASQ----RTAVDEALVCDESFNTHSLEQHD 395 HSP 3 Score: 129.028 bits (323), Expect = 1.897e-29 Identity = 83/307 (27.04%), Postives = 135/307 (43.97%), Query Frame = 3
Query: 3309 FVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQN-SPIPII--KPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSD--------------------------------------------AYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
V HRK+ + +YF+++ +T LFG P+++ + H +Y A + V + SP+P + NHA DC + PF L V ++ C+IC WS+FC GC + N+D +Y + ++ +AIDW ALHL E+ ++ + ++ +P+DL C+ +FT++E L++ Y C CK + A KKL +W LPPILI+HLKRF ++ +W KS K V +P + D ++
Sbjct: 1217 LVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGT---------------------HKDLYCAVWLQVSRLLSPLPATTEQANHAADC--LGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQ--WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYL 1498 HSP 4 Score: 82.8037 bits (203), Expect = 2.586e-15 Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3
Query: 4164 DENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKE-ITMSCAYLLFYERANLNADTFLQQVLS-NVHNTVPQAVEIISDEVESDIKKYCLI 4478
D+ D Y LYA+ H G L GHY++YA N + WY +NDS C+E+ +K I S AYLLFYER L+ + +L + + NT +E+ DE E ++KK C I
Sbjct: 1608 DQFDPRYRLYAVVSHSGMLNGGHYISYASNA-TGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEV--DETEGELKKLCSI 1711
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Match: Usp32 (gene:FBgn0036913 transcript:FBtr0332148) HSP 1 Score: 320.087 bits (819), Expect = 2.091e-88 Identity = 199/559 (35.60%), Postives = 305/559 (54.56%), Query Frame = 3
Query: 1755 NFTILTEDGGVL---SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNG----------LSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKL-LQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNR----------KASV----SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQ-ENLIKFVIYHRKLIQKA 3344
N LT +GG L +P ++ N DFE++P +W + WYG ++ + R + + + V++E+ L L I+ H T V G +S S G + ++ KR +AY A+FS + + E L ++L + E++R+W V +N ++ +ED + L++L +R+ D++L+E RN DLTWPEEL L T + Q ++S+ + G TGL N+GNTCF N+A+Q + NT L +YF R++H E+N N G +G +A+RY EL+K++WT +T + PLKL+ ++KYA QF+G QHD+QELL LLDALHEDLNR+ KP Y ELKDSNGR D VA EAW H A N SI++DLF+G LKS V C C SVRFD +S+LSLP+P++ FE+LV ++P KY N+ D S + ++S S+ PN +L+ + +++I++ LN ++++ Y+++ E S ++ S S E +K + LI A
Sbjct: 443 NVRTLTGEGGHLKRDTPLVQ-NHDFELVPKSLWKALNRWYGDNLPLPRQV--------------------IQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVN----AANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKP-YSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNS-DCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSA 974 HSP 2 Score: 184.496 bits (467), Expect = 2.191e-46 Identity = 127/404 (31.44%), Postives = 211/404 (52.23%), Query Frame = 3
Query: 309 NNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI-ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKD--FNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLIT-----SILSSVEMMTKNYISI--DDLLIWI--NDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYD 1472
T GI+F +L G+V++T+GT EK KF ++LYCN++ + I + +Y+R + SV + + F+ + WI ++ +SKWLL SD V L ++ P YQ +AGVTHL+E +I +LEK + RL N S++ +L L+ PP+P++ + +F AFDEN DG IDF EL CG+SA CRGP ER +FC KIF+ DG + + I + + +E + ++ K +K + DL+ S + +T++ +S+ +D ++W D + P L+L+ ++C IVFGL P+ E I+ WL E++ Y+I W W+ + + + DY K +A++ T + ++ + F + Q+D
Sbjct: 7 GGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVR-----NVNLAPFESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLL-SDNCVSLTSELETPT-FYQSLAGVTHLEEKDIGDLEKEFWRLK-NTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY-KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQ-YTQHTTHTCDYCKRTASQ----RTAVDEALVCDESFNTHSLEQHD 395 HSP 3 Score: 133.265 bits (334), Expect = 1.090e-30 Identity = 85/308 (27.60%), Postives = 137/308 (44.48%), Query Frame = 3
Query: 3309 FVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQN-SPIPII--KPNHALDCD-SVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSD--------------------------------------------AYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
V HRK+ + +YF+++ +T LFG P+++ + H +Y A + V + SP+P + NHA DCD S+ PF L V ++ C+IC WS+FC GC + N+D +Y + ++ +AIDW ALHL E+ ++ + ++ +P+DL C+ +FT++E L++ Y C CK + A KKL +W LPPILI+HLKRF ++ +W KS K V +P + D ++
Sbjct: 1217 LVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGT---------------------HKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQ--WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYL 1501 HSP 4 Score: 82.8037 bits (203), Expect = 2.744e-15 Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3
Query: 4164 DENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKE-ITMSCAYLLFYERANLNADTFLQQVLS-NVHNTVPQAVEIISDEVESDIKKYCLI 4478
D+ D Y LYA+ H G L GHY++YA N + WY +NDS C+E+ +K I S AYLLFYER L+ + +L + + NT +E+ DE E ++KK C I
Sbjct: 1611 DQFDPRYRLYAVVSHSGMLNGGHYISYASNA-TGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEV--DETEGELKKLCSI 1714
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Match: Usp15-31 (gene:FBgn0050421 transcript:FBtr0343495) HSP 1 Score: 201.83 bits (512), Expect = 6.772e-52 Identity = 171/709 (24.12%), Postives = 311/709 (43.86%), Query Frame = 3
Query: 2415 ASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYS-LRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFIC--EQVSRQLSVEPNSILLVSIEDNKIKKFL---NPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRK---------LIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDK----VAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQR--HPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQY--LAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMD-----LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHK---TVRYPVRNLDLNKFIQRDXXXXXXX---------------------XXXXXXXETNRTQPGDENDAIYNLYAINCHLG-SLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSC----AYLLFYERANLNA 4370
A + GV GL N GNTCF N+ +QC+S+T+IL EYF+ D + ++ + + + G +G + + ++K +WT K +D+Y QF + QHDAQE L LLD +HEDLN +K Y+ LK+S GR D+ +A E HI N S V +F +S++ C C+ S FD + +S+ +P QL Q + ++ +P++ + + + S + EQ+ +E + ++LV + + P + + +T+ Y E + + K + T + + L+ AN + A FG P + +D + QD+Q + +A +L ++F A+ ++ P + D D+ ++ HP + ++ A + + + Q+ L ++WS H S + ++ + + ++ A+ +A++P D L +C+E +T E L + ++C C++ K L LW+LP IL++H KRF+ + ++ K V++P+ D++ + R + + ++ S + D D Y+LYA+ H G +L+ GHY N +WY F+D +V E +I AY+LFY+R +++A
Sbjct: 230 ADQTPGVMGLKNHGNTCFMNAVLQCLSHTDILAEYFVLDQYKADLKRRNKINSRKFGTKGELTEQLANVLKALWTCKNESDHSTSFKAVVDRYGTQFRSSTQHDAQEFLFWLLDKVHEDLN-TASKRRYKSLKNSYGRSDEVIAAETLANHIRCNNSFVQAVFQAQFRSSLTCPRCEKQSNTFDPFHCISVQLP-QLTQQTIFVTVVYMTRLPRQVRMGLR-VPAGSPIVALREQLQADTGIEGSRMVLVDLNAEGFTRVFYDTQPVETLSSIETI--YCIEVPAATPVAKAATPSETGDKSAPTAVTPSPAAAQSQADLLLLVANVYRAKDGADITRFGAPFSMKAPRD----CSYQDLQKRMLREMAPLLKPEVFSYATPLAEMFRIRLQDP--------SADPDTYLEQVEHPLLTEMIDM------------------------ALSVLSSEAGPQHIKLLLEWSSPEAHFISKQQDTEEAVVEHESV-ARLSASKPSDTAALTLEQCLEHYTKAETLSAEDAWRCPHCQQYLPVVKTLGLWSLPDILVVHFKRFRQHQSKGPQAAKLTTMVKFPLTAFDMSPHLARGVHESASSSLGMGTGIGLGLGLGSNPWKKARSVDSRSSTLNSRCDAKDTRYDLYAVCYHQGDTLETGHYTAACKNPYDRQWYKFDDQRVSKVPEDDIEQDIINNEAYMLFYQRRSVDA 896
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Match: usp32 (ubiquitin specific peptidase 32 [Source:ZFIN;Acc:ZDB-GENE-100210-5]) HSP 1 Score: 479.174 bits (1232), Expect = 1.471e-142 Identity = 298/832 (35.82%), Postives = 434/832 (52.16%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA--KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE--------------DTEC----------SEINKNGSSPSTQENLI------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L S L +FE +P P+W ++WYGA++S+ RP+ KS V + E++L L+++ L P + SN + P+A KR +AY FS I D+ E LS++L I E+MR+W EN LTL+DDED L+ L + + +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LTEYFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG L+S V C C S RFD ++ LSLP+PM + EI V + + P +Y + N +D + +Q+S S++P ILL + + IK F N ++ + + FE TE + I K+G P+ + + F+ Y HRK+++ YF++ + LFG P+++ + +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + N D ++G Y+A+DW ALHL E+ + ++ +P+ L C+++FT++E L +D LY C KCK + A KKLDLW LPP+LI+HLKRFQF++ RW KS K V++P + D + F+
Sbjct: 532 LTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPV-------IKSTVK--------------AQPELELFPLYLL--FLRQQPATRSPQSNIWVNMGNVPS----------PNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELN----RTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLN-MDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTSKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRA-SLGN----AYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 1299 HSP 2 Score: 259.61 bits (662), Expect = 1.969e-69 Identity = 153/444 (34.46%), Postives = 244/444 (54.95%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKM-LSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDI-EYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSE---KEDYNKDSANKSNQTNTKI 1415
MG +SR+ LSY+EA KR+T+ EL RL+ AF S + ++ F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ ++S + + E + ++ + S+R+ E + ++ + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R +I E + + E K N + + S++ +++ IL + + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + ++I+ W QW + R +N ++ + S +S+Q+ +
Sbjct: 1 MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFG--GTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENML-RSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSV-SEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASV 439 HSP 3 Score: 89.7373 bits (221), Expect = 2.378e-17 Identity = 45/103 (43.69%), Postives = 62/103 (60.19%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+++YNLYAI+CH G + GHYVTYA N + KWY +NDS CKEV EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1464 ESLYNLYAISCHSGIMGGGHYVTYAKNP-NEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTS----MDEDFESDYKKYCVLQ 1561
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Match: usp32 (ubiquitin specific peptidase 32 [Source:ZFIN;Acc:ZDB-GENE-100210-5]) HSP 1 Score: 478.789 bits (1231), Expect = 2.515e-142 Identity = 298/832 (35.82%), Postives = 434/832 (52.16%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA--KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE--------------DTEC----------SEINKNGSSPSTQENLI------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L S L +FE +P P+W ++WYGA++S+ RP+ KS V + E++L L+++ L P + SN + P+A KR +AY FS I D+ E LS++L I E+MR+W EN LTL+DDED L+ L + + +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LTEYFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG L+S V C C S RFD ++ LSLP+PM + EI V + + P +Y + N +D + +Q+S S++P ILL + + IK F N ++ + + FE TE + I K+G P+ + + F+ Y HRK+++ YF++ + LFG P+++ + +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + N D ++G Y+A+DW ALHL E+ + ++ +P+ L C+++FT++E L +D LY C KCK + A KKLDLW LPP+LI+HLKRFQF++ RW KS K V++P + D + F+
Sbjct: 540 LTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPV-------IKSTVK--------------AQPELELFPLYLL--FLRQQPATRSPQSNIWVNMGNVPS----------PNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELN----RTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLN-MDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTSKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRA-SLGN----AYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 1307 HSP 2 Score: 259.61 bits (662), Expect = 1.933e-69 Identity = 153/444 (34.46%), Postives = 244/444 (54.95%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKM-LSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDI-EYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSE---KEDYNKDSANKSNQTNTKI 1415
MG +SR+ LSY+EA KR+T+ EL RL+ AF S + ++ F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ ++S + + E + ++ + S+R+ E + ++ + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R +I E + + E K N + + S++ +++ IL + + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + ++I+ W QW + R +N ++ + S +S+Q+ +
Sbjct: 1 MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFG--GTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENML-RSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSV-SEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASV 439 HSP 3 Score: 89.7373 bits (221), Expect = 2.349e-17 Identity = 45/103 (43.69%), Postives = 62/103 (60.19%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+++YNLYAI+CH G + GHYVTYA N + KWY +NDS CKEV EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1488 ESLYNLYAISCHSGIMGGGHYVTYAKNP-NEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTS----MDEDFESDYKKYCVLQ 1585
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Match: usp11 (ubiquitin specific peptidase 11 [Source:ZFIN;Acc:ZDB-GENE-081104-421]) HSP 1 Score: 234.187 bits (596), Expect = 4.601e-65 Identity = 140/423 (33.10%), Postives = 226/423 (53.43%), Query Frame = 3
Query: 2154 ASFSHRTRITDLIELLSKKLNINR-EEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEIL-VFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
A FS +I + + + + +++ E R+W + + + + + + D CL + +++E RN D TWP Q++ S GV GL+N+GNTCF NSA+QC+SNT LTEYF+++++L E+N +NP G +G +A Y ++IK +W+G +VP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL+D++GR D EVA+EAW+ H+ N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ V +A P +Y ++ +C +S+ V PN + + + +++ RIY + + I + + + + + IY R+ +Y + T LFG+P+ ++V +
Sbjct: 162 AQFSRADKIHAIHQKIRQHFDVSETAESRLWMRSVDTSSERLRNLNMTVLDSCLSSGMTVIMEIRNADGTWPTSRPQIMR-NSADEDSYRGPPGVCGLTNLGNTCFMNSALQCLSNTPPLTEYFLKNLYLEELNF----SNPLGMKGEIAEAYADVIKQMWSGRHYSVVPRVFKTKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKK-EYIELRDADGRPDQEVAEEAWRNHLRRNDSVIVDTFHGLFKSTLVCPECNKVSVTFDPFCYLSVPLPVSKERVMEVFYVSLDPTAKPTQYRLIVPKAGRVLD-LCTALSQATGVPPNQMTVTDVFNHRFY-------RIYQSDESLSCILDRDDIFVYQLSSGCVDEDSDEVVLSIYMRERSHYRDYGSGSNSYGTSLFGHPLRMSVPR 570
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Match: usp11 (ubiquitin specific peptidase 11 [Source:ZFIN;Acc:ZDB-GENE-081104-421]) HSP 1 Score: 234.572 bits (597), Expect = 1.053e-62 Identity = 140/423 (33.10%), Postives = 226/423 (53.43%), Query Frame = 3
Query: 2154 ASFSHRTRITDLIELLSKKLNINR-EEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEIL-VFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
A FS +I + + + + +++ E R+W + + + + + + D CL + +++E RN D TWP Q++ S GV GL+N+GNTCF NSA+QC+SNT LTEYF+++++L E+N +NP G +G +A Y ++IK +W+G +VP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL+D++GR D EVA+EAW+ H+ N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ V +A P +Y ++ +C +S+ V PN + + + +++ RIY + + I + + + + + IY R+ +Y + T LFG+P+ ++V +
Sbjct: 148 AQFSRADKIHAIHQKIRQHFDVSETAESRLWMRSVDTSSERLRNLNMTVLDSCLSSGMTVIMEIRNADGTWPTSRPQIMR-NSADEDSYRGPPGVCGLTNLGNTCFMNSALQCLSNTPPLTEYFLKNLYLEELN----FSNPLGMKGEIAEAYADVIKQMWSGRHYSVVPRVFKTKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKK-EYIELRDADGRPDQEVAEEAWRNHLRRNDSVIVDTFHGLFKSTLVCPECNKVSVTFDPFCYLSVPLPVSKERVMEVFYVSLDPTAKPTQYRLIVPKAGRVLD-LCTALSQATGVPPNQMTVTDVFNHRFY-------RIYQSDESLSCILDRDDIFVYQLSSGCVDEDSDEVVLSIYMRERSHYRDYGSGSNSYGTSLFGHPLRMSVPR 556 HSP 2 Score: 141.739 bits (356), Expect = 1.983e-33 Identity = 91/275 (33.09%), Postives = 138/275 (50.18%), Query Frame = 3
Query: 3564 ALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMD---------LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERAN 4361
A C N+RHP K N NG ++A ++ +F+ + Y+AIDW + +KK+ ++N+ K +Q MD L +CIE FTT E L+++ + C CK+ Q A KKLDLW+LP +LIIHLKRF + +K V +P+R+LD + F+ + N T+P Y+L +++ H G L+ GHY +YA NK++ +WY F+DS E++I S AYLLFY+R +
Sbjct: 706 AASCTPANKRHPGRRRYTERRKKALFTIQAVN-SNGTTERGMAEAGGSI-SFSSQPYIAIDW---------DPDMKKKYY--NENEAEKYIKHQSMDVPHQQTTVQLQECIELFTTVETLEEENPWYCPTCKRHQLATKKLDLWSLPEVLIIHLKRFSYTKYSREKLDTIVEFPLRDLDFSGFLLKKT--------------VNSTEP----PCPYDLISVSNHYGGLRDGHYTSYARNKDNGQWYYFDDSKVTYAREEQIVTSAAYLLFYQRQD 949
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Match: usp4 (ubiquitin specific peptidase 4 (proto-oncogene) [Source:ZFIN;Acc:ZDB-GENE-041008-187]) HSP 1 Score: 211.46 bits (537), Expect = 3.288e-55 Identity = 120/340 (35.29%), Postives = 191/340 (56.18%), Query Frame = 3
Query: 2409 RKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPND---RIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
R+++ G+ GLSN+GNTCF NSA+QC+SNT LTEYF+ D + EIN R NP G +G +A Y +L+K +W ++ + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y L+D+ GR D+ VA+EAW H N SI+VD+FHGL KS + C C SV FD + L+LP+PM+ ++ E+ LV ++ V+ + + + +C +++ V ++++ + +++ K +D +I ++ Y + + +N +Y R+ + A + S T+ LFG P+++TV +
Sbjct: 310 RESAAQPGLCGLSNLGNTCFMNSALQCLSNTPPLTEYFLEDRYEAEIN----RENPLGMRGEIAEAYADLVKQMWLSRSSYVAPRTFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLALRDAEGRPDEIVAKEAWANHRLRNDSIIVDIFHGLFKSTLVCPECSKVSVTFDPFCYLTLPLPMKKDRTMEVYLVRTDPQFRAMQFRVVVPKMGAVAD-LCSALAKLSGVPSENMVVADVYNHRFHKIYKRDDGLNQIMEKDDIFVYEVLEEDSERMN--------------LPVYFRE--RHAKHSGGSSGTM--LFGQPLLITVPR 625 HSP 2 Score: 110.153 bits (274), Expect = 1.102e-23 Identity = 66/175 (37.71%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADT 4376
L +CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +P+R+L++++F+ P + IY+L A++ H G + GHY Y NK KWY F+DS E +I AY+LFY+R +ADT
Sbjct: 836 LRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPRILVVHLKRFSY-NRCWRDKLDTVVDFPIRDLNMSEFV---------------------CDP-KADPYIYDLIAVSNHYGGMGGGHYTAYGKNKADGKWYYFDDSSVSAATEDQIVTKAAYVLFYQRR--DADT 985
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Match: ENSXETT00000054870.1 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:100487551]) HSP 1 Score: 489.189 bits (1258), Expect = 1.889e-145 Identity = 296/845 (35.03%), Postives = 441/845 (52.19%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR------DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA----KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE----DTECSEINKNGSSP-----------------------------------STQENLIKFVI------------------YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L SP+L +D+EI+P P+W ++WYGA++S+ RP+ + ++ + + R+ Q+ + V + +++L + LP + SN I + + PS KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE + S + + P T++NL+ V+ HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D + Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F+
Sbjct: 541 LTLEGGRLKQSPSLLRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNCKTDLPELELFPRYLLFLRQQPVTRQQQSNIWVNMGMMSLRMFPQHLPRGNVRMQQESNSLVLVPKEIYSALLWHFCNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLEGLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWDNHLRRNKSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPIPGSPTSATSPTQTDPNTILLSNGPHSLISNGGLNRPTLIPNGLPNTVVPCGTEKNLVNGVLNGHVSVHPEGPFIGYIIAIHRKMMRTELYFLSFQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCASCPWYRFCRGCKIECSEDR-----AYIGNAYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1354 HSP 2 Score: 234.572 bits (597), Expect = 1.831e-61 Identity = 144/411 (35.04%), Postives = 216/411 (52.55%), Query Frame = 3
Query: 138 YEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFC--SEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNN 1352
Y E+ +T+ EL RLK AF ++ C EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + D S+++ E + +D + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ +S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++E + E+ + ID ++ +IL S + +++++D IW + FLNL+ Q+C IV GLKP EE II WL E + + + ++I+ W QW R ++
Sbjct: 28 YLTVEEPMTDVELKRLKDAFKRTCGGPSYYMNQQCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFSNESGSCVVREEMESMLCMVDGEVP----LSLKKCFAEGEKVNYDKFRNWLLQNKDAFTFSRWLLSGGVFV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHQSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDIDRDGILSRTELEEMVLALLEVWKDNRIDAIPELHLNLSNITDNILQSHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLKPATPEEEGQIIRGWLERESRYGLQFGHNWFLISMQWWQQWKEYVRYDS 431 HSP 3 Score: 88.9669 bits (219), Expect = 6.165e-17 Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N + KWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1548 IYNLYAISCHSGILGGGHYVTYAKNPNA-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1643
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Match: ENSXETT00000054874.1 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:100487551]) HSP 1 Score: 488.419 bits (1256), Expect = 2.231e-145 Identity = 296/841 (35.20%), Postives = 440/841 (52.32%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR------DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLP--SVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE------DTECSEINKNGS---SPS----------------------------TQENLIKFVI------------------YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L SP+L +D+EI+P P+W ++WYGA++S+ RP+ + ++ + + R+ Q+ + V + +++L + LP +VP+ L KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE T + + G +PS T++NL+ V+ HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D + Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F+
Sbjct: 535 LTLEGGRLKQSPSLLRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNCKTDLPELELFPRYLLFLRQQPVTRQQQSNIWVNMGMMSLRMFPQHLPRGNVPSPNAPL-------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLEGLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWDNHLRRNKSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPIPGSPTSATSPTQTGELCPTPSLSGLISNGGLNRPTLIPNGLPNTVVPCGTEKNLVNGVLNGHVSVHPEGPFIGYIIAIHRKMMRTELYFLSFQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCASCPWYRFCRGCKIECSEDR-----AYIGNAYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1319 HSP 2 Score: 232.261 bits (591), Expect = 7.747e-61 Identity = 142/401 (35.41%), Postives = 212/401 (52.87%), Query Frame = 3
Query: 159 ITNKELNRLKRAFNEYSQKKMLSFDKFC--SEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR 1343
+T+ EL RLK AF ++ C EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + D + S+++ E + +D + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ +S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++E + E+ + ID ++ +IL S + +++++D IW + FLNL+ Q+C IV GLKP EE II WL E + + + ++I+ W QW R
Sbjct: 20 VTDVELKRLKDAFKRTCGGPSYYMNQQCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFSNESGSCVVREEMESMLCMVDG----EVPLSLKKCFAEGEKVNYDKFRNWLLQNKDAFTFSRWLLSGGVFV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHQSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDIDRDGILSRTELEEMVLALLEVWKDNRIDAIPELHLNLSNITDNILQSHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLKPATPEEEGQIIRGWLERESRYGLQFGHNWFLISMQWWQQWKEYVR 413 HSP 3 Score: 88.9669 bits (219), Expect = 5.774e-17 Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N + KWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1514 IYNLYAISCHSGILGGGHYVTYAKNPNA-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1609
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Match: ENSXETT00000054888.1 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:100487551]) HSP 1 Score: 483.026 bits (1242), Expect = 5.982e-145 Identity = 291/831 (35.02%), Postives = 430/831 (51.74%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-------DTECSEINKNGSSPS----------------------------TQENLIKFVI------------------YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L SP+L +D+EI+P P+W ++WYGA++S+ RP+ + + + + F R Q + + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE + S +PS T++NL+ V+ HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D + Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F+
Sbjct: 362 LTLEGGRLKQSPSLLRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNCKTDLPELELFPRYLL-FLRQQPVTRQQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLEGLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWDNHLRRNKSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPIPGSPTSATSPTQTGDPTPSLSGLISNGGLNRPTLIPNGLPNTVVPCGTEKNLVNGVLNGHVSVHPEGPFIGYIIAIHRKMMRTELYFLSFQKNRPSLFGMPLIVPC----TVHTRKKDLYDAVWI---------------QVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCASCPWYRFCRGCKIECSEDR-----AYIGNAYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1130 HSP 2 Score: 96.2857 bits (238), Expect = 3.398e-19 Identity = 76/280 (27.14%), Postives = 118/280 (42.14%), Query Frame = 3
Query: 516 ENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNN 1352
E + +D + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ +S+ E C K+F+ DG +R ++E + E+ + ID + +++++D IW + FLNL+ Q+C IV GLKP EE II WL E + + + ++I+ W QW R ++
Sbjct: 21 EGEKVNYDKFRNWLLQNKDAFTFSRWLLSGGVFV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHQSLSE------------------------------------VCFKVFDIDRDGILSRTELEEMVLALLEVWKDNRID---------------------AIPIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLKPATPEEEGQIIRGWLERESRYGLQFGHNWFLISMQWWQQWKEYVRYDS 242 HSP 3 Score: 89.3521 bits (220), Expect = 4.653e-17 Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N + KWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1308 IYNLYAISCHSGILGGGHYVTYAKNPNA-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKM----ADTSSMDEDFESDYKKYCVLQ 1403
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Match: ENSXETT00000054876.1 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:100487551]) HSP 1 Score: 486.108 bits (1250), Expect = 6.467e-145 Identity = 301/877 (34.32%), Postives = 447/877 (50.97%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-------DTECSEINKNGS--SPS--------------TQENLIKFVI------------------YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI----------------QRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLG 4214
LT +GG L SP+L +D+EI+P P+W ++WYGA++S+ RP+ + + + + F R Q + + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE + ++ NG P+ T++NL+ V+ HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D + Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F+ D ++ + N PGD + A N A+N G
Sbjct: 527 LTLEGGRLKQSPSLLRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNCKTDLPELELFPRYLL-FLRQQPVTRQQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLEGLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWDNHLRRNKSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPIPGSPTSPPRDLISNGGLNRPTLIPNGLPNTVVPCGTEKNLVNGVLNGHVSVHPEGPFIGYIIAIHRKMMRTELYFLSFQKNRPSLFGMPLIVPC----TVHTRKKDLYDAVWI---------------QVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCASCPWYRFCRGCKIECSEDR-----AYIGNAYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFLVPKGPIQYQKKQMVLQNEDQSDTRLTQVEGKKELANSVLPGDTDAACRNQPALNPSTG 1341 HSP 2 Score: 233.032 bits (593), Expect = 4.706e-61 Identity = 147/428 (34.35%), Postives = 219/428 (51.17%), Query Frame = 3
Query: 159 ITNKELNRLKRAFNEYSQKKMLSFDKFC--SEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR---------LNNSEKEDYNKDSANKSN 1397
+T+ EL RLK AF ++ C EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + D S+++ E + +D + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ +S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++E + E+ + ID ++ +IL S + +++++D IW + FLNL+ Q+C IV GLKP EE II WL E + + + ++I+ W QW R L+ + D +D SN
Sbjct: 20 VTDVELKRLKDAFKRTCGGPSYYMNQQCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFSNESGSCVVREEMESMLCMVDGEVP----LSLKKCFAEGEKVNYDKFRNWLLQNKDAFTFSRWLLSGGVFV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHQSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDIDRDGILSRTELEEMVLALLEVWKDNRIDAIPELHLNLSNITDNILQSHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLKPATPEEEGQIIRGWLERESRYGLQFGHNWFLISMQWWQQWKEYVRYVSVPGGRNLSGTTSPDQKEDDRTGSN 440 HSP 3 Score: 88.9669 bits (219), Expect = 5.780e-17 Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N + KWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1477 IYNLYAISCHSGILGGGHYVTYAKNPNA-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1572
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Match: ENSXETT00000054867.1 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:100487551]) HSP 1 Score: 485.723 bits (1249), Expect = 1.396e-144 Identity = 293/831 (35.26%), Postives = 429/831 (51.62%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE------DTECSEINKNGSSPSTQENLIK-------------------------------------------FVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L SP+L +D+EI+P P+W ++WYGA++S+ RP+ + + + + F R Q + + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE T + + G ST NLI F+ Y HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D + Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F+
Sbjct: 536 LTLEGGRLKQSPSLLRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNCKTDLPELELFPRYLL-FLRQQPVTRQQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLEGLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWDNHLRRNKSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPIPGSPTSATSPTQTGDPFSTSPNLISNGGLNRPTLIPNGLPNTVVPCGTEKNLVNGVLNGHVSVHPEGPFIGYIIAIHRKMMRTELYFLSFQKNRPSLFGMPLIVPC----TVHTRKKDLYDAVWI---------------QVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCASCPWYRFCRGCKIECSEDR-----AYIGNAYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1304 HSP 2 Score: 253.062 bits (645), Expect = 2.795e-67 Identity = 152/423 (35.93%), Postives = 228/423 (53.90%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFC--SEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNN 1352
MG +SR+ L+Y+EA KR+T+ EL RLK AF ++ C EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + D + S+++ E + +D + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ +S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++E + E+ + ID ++ +IL S + +++++D IW + FLNL+ Q+C IV GLKP EE II WL E + + + ++I+ W QW R ++
Sbjct: 1 MGAKESRIGFLTYDEAVKRVTDVELKRLKDAFKRTCGGPSYYMNQQCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFSNESGSCVVREEMESMLCMVDG----EVPLSLKKCFAEGEKVNYDKFRNWLLQNKDAFTFSRWLLSGGVFV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHQSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDIDRDGILSRTELEEMVLALLEVWKDNRIDAIPELHLNLSNITDNILQSHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLKPATPEEEGQIIRGWLERESRYGLQFGHNWFLISMQWWQQWKEYVRYDS 416 HSP 3 Score: 89.3521 bits (220), Expect = 5.473e-17 Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N + KWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1499 IYNLYAISCHSGILGGGHYVTYAKNPNA-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1594
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Match: Usp32 (ubiquitin specific peptidase 32 [Source:MGI Symbol;Acc:MGI:2144475]) HSP 1 Score: 469.929 bits (1208), Expect = 5.034e-139 Identity = 287/838 (34.25%), Postives = 429/838 (51.19%), Query Frame = 3
Query: 1761 TILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR-DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE--------------DTECSE---------INKNGSSP----------------STQENLIKFVI------------------YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
T LT +GG L +P L D+E++P P+W ++WYG+++++ RP+ ++ DI + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++ IL + + IK F N ++ L+ + + FE T+CS + NG P T++N+ ++ HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D F +A+DW ALHL E+ + + ++ +P++L C+ +FT++E L ++ +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P + D + F+
Sbjct: 538 TSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPE--LELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLN-MDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDR-----AFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 1312 HSP 2 Score: 253.447 bits (646), Expect = 1.550e-67 Identity = 150/414 (36.23%), Postives = 224/414 (54.11%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEY-SQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LSYEEA +R+T+ EL RLK AF +S F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ +ES S + E+E + + D ++R+ E + ++ + W+ N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R +++ + E+ + D D++ IL++ + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + +II+ W QW
Sbjct: 1 MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMSQHCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPD----TLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQW 407 HSP 3 Score: 88.1965 bits (217), Expect = 8.878e-17 Identity = 46/101 (45.54%), Postives = 59/101 (58.42%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N KWY +NDS CKE+ EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1509 IYNLYAISCHSGILGGGHYVTYAKNPNC-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1604
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Match: Usp4 (ubiquitin specific peptidase 4 (proto-oncogene) [Source:MGI Symbol;Acc:MGI:98905]) HSP 1 Score: 333.183 bits (853), Expect = 5.831e-96 Identity = 261/880 (29.66%), Postives = 414/880 (47.05%), Query Frame = 3
Query: 1815 DFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINRE-EMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQL------VTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRI-YLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIA-EITNQDIQDKVAHI----LNNQIFHSHVYP----ADQVSVKQNSPIPI---------IKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPN-NSDAYNT-VGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
D+ ++P WN NWYG + ++PI R + +H + + KVE+ LL L + ++ P+ N LS FS I + + + K NI E E R+W N + D +QD L +++E +N D TWP + Q + + + G+ GL N+GNTCF NSA+QC+SNT LTEYF++D + EIN R NP G +G +A Y ELIK +W+G + P K + ++A QFSG QQ D+QELL+ +LD LHEDLNR+ KP Y E KD+NGR D VA+EAW+ H N S++VD FHGL KS + C C SV FD + L+LP+P++ ++ E+ LV P +Y V + + S +CE +S+ + ++++ + +++ K ++ + ++T +++ E + +GS I +Y R+ +K+ A S V L+G P++++V K + E Q + D+++ L ++ S + P + S + + + + D H +V +H + +S N C + NS A + + N LAIDW L + +K LS + + K A + L +CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +PVR L++++F+ +R+ +Y+L A++ H G++ +GHY YA N+ + KWY F+DS E +I AY+LFY+R
Sbjct: 92 DYVLVPAEAWNKLLNWYGC-VEGQQPI---VRKVV-------EHGLFVKH----CKVEVYLLELKLCENSDPT-----NVLS-------------------------------CHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQGQVLVIEPQNEDGTWPRQSLQSNGSGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTEYFLKDEYEAEIN----RDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKP-YLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPQCRPIQYRVTVPLMGAISD-LCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVY---EVCNTSMDGS------ECITLPVYFRE--KKSRPSSASSGAV--LYGQPLLVSVPKHKLTLESLYQAVCDRISRYIKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEVEGSGEDDQGDDHSESAQKVKGQPRHKRLFTFS-LVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHLSMSQPQKKKKAA---VALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSY-NRYWRDKLDTVVEFPVRALNMSEFV------------------CDRSA----RPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQR 874
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Match: Usp4 (ubiquitin specific peptidase 4 (proto-oncogene) [Source:MGI Symbol;Acc:MGI:98905]) HSP 1 Score: 313.538 bits (802), Expect = 6.327e-89 Identity = 217/671 (32.34%), Postives = 342/671 (50.97%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRI-YLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIA-EITNQDIQDKVAHI----LNNQIFHSHVYP----ADQVSVKQNSPIPI---------IKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPN-NSDAYNT-VGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
+ G+ GL N+GNTCF NSA+QC+SNT LTEYF++D + EIN R NP G +G +A Y ELIK +W+G + P K + ++A QFSG QQ D+QELL+ +LD LHEDLNR+ KP Y E KD+NGR D VA+EAW+ H N S++VD FHGL KS + C C SV FD + L+LP+P++ ++ E+ LV P +Y V + + S +CE +S+ + ++++ + +++ K ++ + ++T +++ E + +GS I +Y R+ +K+ A S V L+G P++++V K + E Q + D+++ L ++ S + P + S + + + + D H +V +H + +S N C + NS A + + N LAIDW L + +K LS + + K A + L +CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +PVR L++++F+ +R+ +Y+L A++ H G++ +GHY YA N+ + KWY F+DS E +I AY+LFY+R
Sbjct: 297 IQPGLCGLGNLGNTCFMNSALQCLSNTAPLTEYFLKDEYEAEIN----RDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKP-YLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPQCRPIQYRVTVPLMGAISD-LCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVY---EVCNTSMDGS------ECITLPVYFRE--KKSRPSSASSGAV--LYGQPLLVSVPKHKLTLESLYQAVCDRISRYIKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEVEGSGEDDQGDDHSESAQKVKGQPRHKRLFTFS-LVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHLSMSQPQKKKKAA---VALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSY-NRYWRDKLDTVVEFPVRALNMSEFV------------------CDRSA----RPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQR 921
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Match: Usp32 (ubiquitin specific peptidase 32 [Source:MGI Symbol;Acc:MGI:2144475]) HSP 1 Score: 259.225 bits (661), Expect = 1.984e-77 Identity = 133/279 (47.67%), Postives = 179/279 (64.16%), Query Frame = 3
Query: 2343 RNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIK 3179
RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++ IL + + IK
Sbjct: 2 RNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLN-MDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIK 274
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Match: Usp15 (ubiquitin specific peptidase 15 [Source:MGI Symbol;Acc:MGI:101857]) HSP 1 Score: 233.032 bits (593), Expect = 2.707e-62 Identity = 155/443 (34.99%), Postives = 233/443 (52.60%), Query Frame = 3
Query: 2160 FSHRTRITDLIELLSKKLNI-NREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQ---------LVTLKSLQNRKASVSK------GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE--DTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAE 3431
FS I + + + K NI + +E R+W N ++ D +QD L +++EQ+N D TWP + L + + +N S G+ GLSN+GNTCF NSAIQC+SNT LTEYF+ D + E+N NP G +G +A Y ELIK +W+G + + P K + ++A QFSG QQ D QELL+ LLD LHEDLNRI KP Y +LKD++GR D VA+EAW+ H+ N SI+VD+FHGL KS + C C SV FD + L+LP+PM+ + E+ LV A P +Y V+ I + +C +S V + +++ I +++ RI+ + I E D EIN N + T+ +I + R+ + ++Y T + LFG P ++ + +++ +
Sbjct: 152 FSKADTIDTIEKEIRKIFNIPDEKEARLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELN----FDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKP-YIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPMQYKVIVPKIGNILD-LCTALSALSGVPADKMIVTDIYNHRFH-------RIFAVDENLSSIMERDDIYVFEININ-RAEDTEHVVIPVCL--REKFRHSSY---THHTGSSLFGQPFLMAIPRNNTED 575 HSP 2 Score: 112.079 bits (279), Expect = 2.997e-24 Identity = 72/224 (32.14%), Postives = 110/224 (49.11%), Query Frame = 3
Query: 3717 FNDEQYLAIDWS--MAALHLSSNIK--FEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADT 4376
++ +LA+DW + + N FEK ++ K + L CIE FTT+E L + + C CK+ Q+A KKLDLW+LPP+L++HLKRF + K V +P+ +LD+++F+ P + YNL A++ H G + GHY +A NK+ KWY F+DS E +I AY+LFY+R + + T
Sbjct: 714 LDERSFLALDWDPDLKKRYFDENAAEDFEKH-----ESVEYKPPKRPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFL---------------------INP-NAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGT 910
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Match: sp|Q8NFA0|UBP32_HUMAN (Ubiquitin carboxyl-terminal hydrolase 32 OS=Homo sapiens OX=9606 GN=USP32 PE=1 SV=1) HSP 1 Score: 471.855 bits (1213), Expect = 8.398e-139 Identity = 292/838 (34.84%), Postives = 427/838 (50.95%), Query Frame = 3
Query: 1761 TILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETR-DISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-DTECSEINKN-------GSSPSTQENLI---------------------------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
T LT +GG L +P L D+E++P P+W ++WYGA++++ RP+ ++ DI + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW H+ N SIVVDLFHG L+S V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S + ILL + + IK F N ++ L+ + + FE S I+ + SSPST E F Y HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D F Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L ++ +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P + D + F+
Sbjct: 538 TSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPE--LELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDR-----AFIGNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL 1312 HSP 2 Score: 248.054 bits (632), Expect = 6.367e-65 Identity = 147/414 (35.51%), Postives = 221/414 (53.38%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEY-SQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LSYEEA +R+T+ EL RLK AF + F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ +ES + + E+E + + D ++R+ E + ++ + W+ N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++ + E+ + D D++ IL++ + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + + +II+ W QW
Sbjct: 1 MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFIREVLGDGVPPKVAEVIYCSFGG--TSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGNYVIREEMERMLHVVDGKVPD----TLRKCFSEGEKVNYEKFRNWLFLNKDAFTFSRWLLSGGVYV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLEVWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQW 407 HSP 3 Score: 87.4261 bits (215), Expect = 9.963e-16 Identity = 46/101 (45.54%), Postives = 59/101 (58.42%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
IYNLYAI+CH G L GHYVTYA N KWY +NDS CKE+ EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1509 IYNLYAISCHSGILGGGHYVTYAKNPNC-KWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1604
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Match: sp|P51784|UBP11_HUMAN (Ubiquitin carboxyl-terminal hydrolase 11 OS=Homo sapiens OX=9606 GN=USP11 PE=1 SV=3) HSP 1 Score: 326.635 bits (836), Expect = 1.549e-92 Identity = 231/773 (29.88%), Postives = 362/773 (46.83%), Query Frame = 3
Query: 2145 AYRASFSHRTRITDLIELLSKKLNIN-REEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPE-ELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLN---PNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSH------------------VYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVL------AQVNSNIKHCSICHWSNF-CNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDA---IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANL 4364
++ FSH I ++ ++ + +E+ R+W E +L + D + D L I++E R D TWP +L+ + S ++ G+ GL+N+GNTCF NSA+QC+SN LTEYF+ + +L E+N NP G +G +A Y +L+K W+G IVP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL D+ GR D EVAQEAWQ H N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ + L S +C +S+ + P +++ + ++ K P I ++ Y E++ + I +Y R+ +Y ++ + LFG+P++++V +D T + + + + + L+ + + P P +P A V R PF+L +Q + + NG SD + + + Y+AIDW + + ++ D A P+ L +CIE FTT E L+K+ + C CK+ Q A KKLDLW LP ILIIHLKRF + +K V +P+R+LD ++F+ QP +E++ Y+L A++ H G ++ GHY T+A NK+S +W+ F+D+ V+E +I AY+LFY+R ++
Sbjct: 210 SHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNF----RNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKK-EYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVY--------EVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLM--LFGHPLLVSVPRD---RFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDP----EPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNG-----TSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKA--PVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFV---------------------IQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDV 932
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Match: sp|P35123|UBP4_MOUSE (Ubiquitin carboxyl-terminal hydrolase 4 OS=Mus musculus OX=10090 GN=Usp4 PE=1 SV=3) HSP 1 Score: 313.538 bits (802), Expect = 4.426e-88 Identity = 217/671 (32.34%), Postives = 342/671 (50.97%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRI-YLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIA-EITNQDIQDKVAHI----LNNQIFHSHVYP----ADQVSVKQNSPIPI---------IKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPN-NSDAYNT-VGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
+ G+ GL N+GNTCF NSA+QC+SNT LTEYF++D + EIN R NP G +G +A Y ELIK +W+G + P K + ++A QFSG QQ D+QELL+ +LD LHEDLNR+ KP Y E KD+NGR D VA+EAW+ H N S++VD FHGL KS + C C SV FD + L+LP+P++ ++ E+ LV P +Y V + + S +CE +S+ + ++++ + +++ K ++ + ++T +++ E + +GS I +Y R+ +K+ A S V L+G P++++V K + E Q + D+++ L ++ S + P + S + + + + D H +V +H + +S N C + NS A + + N LAIDW L + +K LS + + K A + L +CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +PVR L++++F+ +R+ +Y+L A++ H G++ +GHY YA N+ + KWY F+DS E +I AY+LFY+R
Sbjct: 297 IQPGLCGLGNLGNTCFMNSALQCLSNTAPLTEYFLKDEYEAEIN----RDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKP-YLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPQCRPIQYRVTVPLMGAISD-LCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVY---EVCNTSMDGS------ECITLPVYFRE--KKSRPSSASSGAV--LYGQPLLVSVPKHKLTLESLYQAVCDRISRYIKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEVEGSGEDDQGDDHSESAQKVKGQPRHKRLFTFS-LVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHLSMSQPQKKKKAA---VALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSY-NRYWRDKLDTVVEFPVRALNMSEFV------------------CDRSA----RPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQR 921
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Match: sp|A6QR55|UBP4_BOVIN (Ubiquitin carboxyl-terminal hydrolase 4 OS=Bos taurus OX=9913 GN=USP4 PE=2 SV=1) HSP 1 Score: 311.997 bits (798), Expect = 1.647e-87 Identity = 215/681 (31.57%), Postives = 332/681 (48.75%), Query Frame = 3
Query: 2421 VSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLN---------PNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIA-EITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIK----HCSICHWSNFCNGCPLPNNSDAY---NTVGT--------------FNDEQYLAIDW---SMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
V G+ GL N+GNTCF NSA+QC+SNT LT+YF++D + EIN R NP G +G +A Y ELIK +W+G + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y ELKD+NGR D VA+EAW+ H N S++VD FHGL KS + C C SV FD + L+LP+P++ ++ EI LV P +Y V+ + + S +CE +S+ + ++++ + +++ K P D I++ + CS +SP E + +Y R+ + + + T L+G P++++V K + E Q + ++++ + + ++ S + R + + + + H N G P P + N+ GT N LAIDW + + + + +K +S + + K TA + L CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +PVR L++++F+ P +Y+L A++ H G++ +GHY YA NK + KWY F+DS E +I AY+LFY+R
Sbjct: 297 VQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEIN----RDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSD-LCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEV----------CS------TSPDGSE-CVTLPVYFRERKSRP----SSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEEMEHQEEGREQLSETEGSGDDEPGSDHGEATQKKN--KGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSCYYNEQESETYEKHVSMLQPQKKKKTA---VALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSY-NRYWRDKLDTVVEFPVRGLNMSEFV---------------------CDP-SARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLACEDQIVTKAAYVLFYQR 922
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Match: sp|M9PD06|UBP32_DROME (Ubiquitin carboxyl-terminal hydrolase 32 OS=Drosophila melanogaster OX=7227 GN=Usp32 PE=3 SV=1) HSP 1 Score: 320.087 bits (819), Expect = 2.096e-87 Identity = 199/559 (35.60%), Postives = 305/559 (54.56%), Query Frame = 3
Query: 1755 NFTILTEDGGVL---SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNG----------LSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKL-LQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNR----------KASV----SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQ-ENLIKFVIYHRKLIQKA 3344
N LT +GG L +P ++ N DFE++P +W + WYG ++ + R + + + V++E+ L L I+ H T V G +S S G + ++ KR +AY A+FS + + E L ++L + E++R+W V +N ++ +ED + L++L +R+ D++L+E RN DLTWPEEL L T + Q ++S+ + G TGL N+GNTCF N+A+Q + NT L +YF R++H E+N N G +G +A+RY EL+K++WT +T + PLKL+ ++KYA QF+G QHD+QELL LLDALHEDLNR+ KP Y ELKDSNGR D VA EAW H A N SI++DLF+G LKS V C C SVRFD +S+LSLP+P++ FE+LV ++P KY N+ D S + ++S S+ PN +L+ + +++I++ LN ++++ Y+++ E S ++ S S E +K + LI A
Sbjct: 443 NVRTLTGEGGHLKRDTPLVQ-NHDFELVPKSLWKALNRWYGDNLPLPRQV--------------------IQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVN----AANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKP-YSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNS-DCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSA 974 HSP 2 Score: 184.496 bits (467), Expect = 2.196e-45 Identity = 127/404 (31.44%), Postives = 211/404 (52.23%), Query Frame = 3
Query: 309 NNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI-ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKD--FNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLIT-----SILSSVEMMTKNYISI--DDLLIWI--NDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYD 1472
T GI+F +L G+V++T+GT EK KF ++LYCN++ + I + +Y+R + SV + + F+ + WI ++ +SKWLL SD V L ++ P YQ +AGVTHL+E +I +LEK + RL N S++ +L L+ PP+P++ + +F AFDEN DG IDF EL CG+SA CRGP ER +FC KIF+ DG + + I + + +E + ++ K +K + DL+ S + +T++ +S+ +D ++W D + P L+L+ ++C IVFGL P+ E I+ WL E++ Y+I W W+ + + + DY K +A++ T + ++ + F + Q+D
Sbjct: 7 GGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVR-----NVNLAPFESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLL-SDNCVSLTSELETPT-FYQSLAGVTHLEEKDIGDLEKEFWRLK-NTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY-KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQ-YTQHTTHTCDYCKRTASQ----RTAVDEALVCDESFNTHSLEQHD 395 HSP 3 Score: 133.265 bits (334), Expect = 1.092e-29 Identity = 85/308 (27.60%), Postives = 137/308 (44.48%), Query Frame = 3
Query: 3309 FVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQN-SPIPII--KPNHALDCD-SVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSD--------------------------------------------AYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
V HRK+ + +YF+++ +T LFG P+++ + H +Y A + V + SP+P + NHA DCD S+ PF L V ++ C+IC WS+FC GC + N+D +Y + ++ +AIDW ALHL E+ ++ + ++ +P+DL C+ +FT++E L++ Y C CK + A KKL +W LPPILI+HLKRF ++ +W KS K V +P + D ++
Sbjct: 1217 LVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGT---------------------HKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQ--WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYL 1501 HSP 4 Score: 82.8037 bits (203), Expect = 2.751e-14 Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3
Query: 4164 DENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKE-ITMSCAYLLFYERANLNADTFLQQVLS-NVHNTVPQAVEIISDEVESDIKKYCLI 4478
D+ D Y LYA+ H G L GHY++YA N + WY +NDS C+E+ +K I S AYLLFYER L+ + +L + + NT +E+ DE E ++KK C I
Sbjct: 1611 DQFDPRYRLYAVVSHSGMLNGGHYISYASNA-TGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEV--DETEGELKKLCSI 1714
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Match: A0A4W4G5G8 (Uncharacterized protein OS=Electrophorus electricus OX=8005 PE=3 SV=1) HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0 Identity = 477/1436 (33.22%), Postives = 718/1436 (50.00%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEY-SQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR---LNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIK--LLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA--KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE---------DTECSEINKNGSSPSTQEN-----------------------------------------LIKFVIY-HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI-QRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINC 4205
MG +SR+ LSY+EA KR+T+ EL RLK AF +S F EV G +P +S I+ + T G++F L +G+V+LT+G EEK ++ FSL+ +ES S E I A + + S+R+ E + ++ + W+ +N S+WLL+S V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ SI E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG R ++ + E+ + D D++ IL + K +++++D IW FLNL+ Q+C IV GL+P EE II WL E + + P D+ ++I W QW + + + + + + ++ Q + +S S F+ N ++ +++A + + + + D + Q+ +G D + RP + + LT +GG+L SP LR +DFE++P P+W ++WYGA++S+ RP+ RD+ K+ + + F R Q + ++ H + P A +++++ PSA KR + Y FS I D+ + LS++L I E+MR+W I+EN LTL+DDED L+ L + + +++E RN D++WPEE++ L + K KG TGLSN+GNTCF NS+IQC+SNT LTEYF HL E+N RTNP G +G +A YG+LI+++W G+ I PLKL+ T+ KYA +F G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG L+S V C C S RFD ++ LSLP+PM + EI+V + P +Y + N +D S + +S ++P +LLV I + IK F N ++ + + FE T E +SPS + +VI HRK+++ YF++ + LFG P+++ +A+ +D+ + V QVS + SP+P +P NHA DC DS+ ++PF L V + C+ C W FC GC + N D VG +A+DW ALHL +S + ++ +S + ++ A++ +P+ L C+ +FTT+E L ++ LY C KC+ + A KKLD+W LPPILI+HLKRFQF++ RW KS K VR+P + D + F+ R+ ++ S E + + G + N+YA+ C
Sbjct: 1 MGAKESRIGFLSYDEAVKRVTDVELKRLKDAFRRTCGLSYFMSQQCFFKEVLGDMVPLNISEVIYSSFG--GTSKGLHFNNLIVGLVLLTRGRDEEKARYIFSLFASESGSYATREDIE-AMLQAVDGEVHPSLRKCFTEAEKVNYEKFKNWLLQNKDAFTFSRWLLSSGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFIPLVSPPIHPSISEGLFHAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDGDRDGVLCRQELHEMVGALLEVWRDNRTDFIPELQANVPDIVEDILRVHDTTQKGHLTLEDYQIWSVQSALAAEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPGDYWFLICTQWWQQWKDYVKYPPVFAAGRSPPSTSGLDQGGQGDCAVSLHSSPEEKFSDN------VSSASEASETTSTGHSTATDVCFARQPDSPEADAQSF------LGAD------------------GSATALRPGAIDNQPLVTSEPLKA-------PTLTMEGGLLRRSPPLRLGQDFEVVPEPVWRALYHWYGANLSLPRPV-IRARDMEKAELELFPRHLLFLRQQPPARTPQSNSWSSIGRTGHPIFHWPPAAPLVTSWSMPS---------------PSAPLKRVLVYTGCFSRVGTIKDIHDYLSQRLRIKEEDMRLWLFINENYLTLLDDEDHSLEALKIPDEQYMVIEVRNKDMSWPEEMSFLANSSRMNRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFTSGCHLYELN----RTNPIGMRGHMAKCYGDLIQELWNGTQKSIAPLKLRWTIAKYAPRFDGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVASEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHVSSRFDPFNFLSLPLPMDNSMHLEIIVVRLDGSTPVRYGLRLN-VDERYSGLKSLLSELCHLKPEQLLLVEIHLSCIKNFPLDNQKVRRAVSGFLCAFEIPLPTFPSSATSHKEAGTGENSPSLSNSTSLRLDIIPKGMPNTVVPCSADGSPANGLPNGHVPTPHAGPFTGYVIAIHRKMMRAELYFLSSQKNRPSLFGMPLIVPC----MADTRGRDLYNTV---------------WTQVS-RLASPLPPQEPCNHAQDCDDSLGYQYPFTLRAVQGDGNSCAWCPWYRFCRGCTIECN-DGRAAVGHV----CIAVDWDPTALHLRYQTSQERLVEEHVSVELSRRAQA---EPISLDSCLRAFTTEEELGENELYYCSKCQTHRVATKKLDIWRLPPILIVHLKRFQFVNGRWIKSQKIVRFPRDSFDPSAFLTPREAEHSQHGWRSCSKEELSEQETGPNSG---NIYALVC 1342 HSP 2 Score: 87.0409 bits (214), Expect = 3.940e-13 Identity = 42/103 (40.78%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ +Y+LYAI+CH G L GHYVTYA N + KWY +NDS CKE++ +EI AY+LFYE+ ++ F+ + + ++ E+D KKYC++Q
Sbjct: 1429 EPVYDLYAISCHSGILGGGHYVTYAKNP-NEKWYCYNDSSCKEMNPEEIDTDSAYILFYEQQGVDYSLFMPKTDGKKMTDTSS----MDEDFETDYKKYCVLQ 1526
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Match: K1RE00 (Ubiquitin carboxyl-terminal hydrolase 32 OS=Crassostrea gigas OX=29159 GN=CGI_10017491 PE=3 SV=1) HSP 1 Score: 720.309 bits (1858), Expect = 0.000e+0 Identity = 518/1604 (32.29%), Postives = 782/1604 (48.75%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQ-KKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVF-NLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGD----IEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHV-YIINKSWMNQWIN---------SFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNN--VFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENN-LTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPND--RIYLTQTLYAYIFE----------DTECSEINKNGSSPSTQENLIKFVIYHRKLIQK------------------ANYFMAHSR---------------------TVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILN------------NQIFHSHVYPADQVSVKQNSPIPIIK---PNHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEML---DKDTLYQ----------CFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDEN----------------DAIYNLYAIN-----CHL----------------------GSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
MG +S+L +EEA KR+ + E+ R K AF + +S FC EV G +P+ ++N +++ T G++ K+L G+VILTKG+ EEK K F +Y ++S S ++ E + ++ + +A + EN + FD + W+K++P E++KWLL V L D P YQ +AGVTHL+E +I ELEK Y L + S F+L F +VCPP+PE+I E +F AFDEN D IDF E+ CGISACCRGP TER+KFC K+F+ DGK + ++ + +I +E ++S++ + IL+S + IS + L+W + D FL+L++QIC +V GLKP++ EE II QWL E K K V Y+I+ W N WI + RL+N E + N + S ++ ++ V +N I K + Y Q+NG T S + +S + ++ S + P K I+N NL+ NN K+ S LT +GG L L +DFE++P IW WYG +I++ R + IS + ++E+ +T+ +++H + + A + + P +R +A+ A+FS + I + E L K+ RE++R+WK+ ++++ +TL++DE ++D + IL+E RN DLTWPEE++QL K+ + + KG TGL+N+GNTCF N+A+QC+SNT LT+YFI +HL E+N R+NP G +G +A RYGEL+KD+W+G++ I PLKL+ T+ KYA +F+G QQHD+QELLS LLD LHEDLNR+ KP Y ELKDS+GR D+ VA+EAW+ H+ N SI+VDLFHG LKS V C C SVRFD ++ LSLP+PM E++V ++P KY + N +D + +++SR S+ P+ +L V I +K N + + LYAY + E + KN S N I+ + +K+ QK AN HSR +ELF ++ RK ++ ++ Q + NQ + V+ QVS + SP+P + NHA DC DS+ +PF L V + C+ C W FC GC + N++ +N G+F LAIDW ALHL E+ + D ++++ +P++L C+++FT + ++T Y C KCK I++LKRFQFL+ RW KSHK V +PV + D + ++ +++ + + + T EN D Y+ ++N C L G L GHYV YA N + KWY +NDS CKE +++ + AY+LFYER N++ F + +V P E I DE ESD+KK C++Q
Sbjct: 1 MGAKESKLQYFPFEEACKRMKDTEMKRSKDAFKRVATLGGQMSDIVFCREVLGDGVPAGIANLLYRGFG--GTSKGLSLKDLLCGLVILTKGSQEEKIKLLFGMYADDSYSYVQKEEMDRLVLESEGQISVA-LSDLFQENDQVSFDYFRLWVKKHPDATEMTKWLLMEHMTVSLSDDTDTPT-FYQTLAGVTHLEETDISELEKRYWTLKSQSKSGKFDLDTFKAMVCPPVPEAICESLFHAFDENRDNHIDFKEMACGISACCRGPGTERQKFCFKVFDVNHDGKLCERELVQMVQALLRIRQENHPSDSMECDPFYKMEPETVAREILASHDSDKDGCISQAEYLVWTVNNALADNFLDLLSQICHLVMGLKPQSKEEEGEIIKQWLQRESK-KAFEVGQVWYLISMGWWNSWIEYVTPQIQLVNGRLDNGEGDRVNGTETLHKITKGKQASKALPWDDDSVIIVNM-----ITKGKNSEY----NTQLNGSLKSSTIPQRSQSACSSPIKTCLSASDSNGFTSVSPGPSPKLRKKGLPQTNALPVKPGPIDNSNLITPNNSKVVS----LTAEGGRLRRDTMLVRGKDFELVPEVIWRALSTWYGGNIALPRTV------------------ISSKNNNGNPELELYPITVKLLRHQIVAQRPAPTSFTGVMSGIGGMALSMTNYVSSAPPIPRRYVAFTATFSKQHTIQQIYEFLCTKVRFIREDIRLWKIGNKDDIMTLLEDESMTVEDCVIEENQSILIEVRNKDLTWPEEISQLAKNKTCRKEQVPTQKGATGLNNLGNTCFMNAAVQCVSNTWPLTQYFIGGLHLFELN----RSNPLGMKGHIAQRYGELVKDLWSGTSKTIAPLKLRWTIGKYAPRFNGFQQHDSQELLSFLLDGLHEDLNRVHVKP-YVELKDSDGREDEIVAEEAWENHLRRNQSIIVDLFHGQLKSQVRCKECGLLSVRFDPFNYLSLPLPMDSCIHLEVIVTRLDGSVPIKYGIRLN-MDEKYKALKKELSRLTSIPPDQVLFVEIMGPIVKALPKDNQMVKTLVGGVLYAYELPPHEEIKPMTIEEEKGTVLKN----SVGLNDIQRGLATKKIQQKLENGPSSVSNALTVEDSSANNTPTHSRHPSDVPTMGSLQPTPENPAPKPSSELFSGFVIGVHRKMNLMDVYFLSSQKARPGLFGTPIILPCVEDTTNQDLYQFVW--TQVS-RLVSPLPPSESKSANHAQDCDDSLGYEYPFTLKVVKKDGFSCAWCPWYRFCRGCKVECNAEDFNFGGSF-----LAIDWEPTALHLRYQTALERLYEDHDSVEDSRRKQTEPINLDTCLQAFTIWRLTLPGLQETRYMEASFHTGKCDCSKCK------------------IVNLKRFQFLNGRWVKSHKIVNFPVEDFDPSNYLAPRVSSSDDDKQSVTCVCQSETSAVVENVPNGDIPNHREDQTHGDMTYSPDSLNSPCMKCQLTRNIPNDESPIKYDLSSMSCHTGILGGGHYVCYAKNP-NQKWYCYNDSSCKECSVEQMDCNSAYILFYERQNIDFSHF----MPDVEGKEPDLQE-IDDEFESDLKKACVLQ 1526
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Match: A0A151N6Q3 (Ubiquitin carboxyl-terminal hydrolase 32 isoform A OS=Alligator mississippiensis OX=8496 GN=USP32 PE=3 SV=1) HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0 Identity = 475/1411 (33.66%), Postives = 707/1411 (50.11%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW------------INSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE------DTECS-----------------EINKNGSSP----------------STQENL-----------------IKFVI-YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKF-IQRD 4097
MG +SR+ LSY+EA +R+T+ EL RLK AF +S F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + + D ++++ E + ++ + W+ N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R +++ + E+ + DK +++ IL++ + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + ++I SW QW I S + N K S + S Q K+ +N A K +I+ +++A +++ M + + + SD+ Q + ++S G P K I+NQ L+ K AS LT +GG L SP L D+ ++P P+W ++WYGA++S+ RP+ ++ + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN+L+L+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ I PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE T S I NG P T+ NL I ++I HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D VG Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F +Q+D
Sbjct: 1 MGAKESRIGFLSYDEALRRVTDVELKRLKDAFKRTCGLSCYMSQQCFIREVLGDGVPPKVAEVIYCSFGG--TAKGLHFNNLIVGLVLLTRGKEEEKAKYIFSLFSNESGSYVVREEMERMLHVVDGKVPD----TLKKCFSEGEKVNYEKFKNWLLHNKDAFTFSRWLLSGGVYV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIHPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRTELKEMVVALLEVWKDNRTDKIPELDKDLSEIVEDILNAHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFLIAVSWWQQWKEYVKYDANLVMIESSFVLNGGKNAVIAASTHASEQGEEKMG---GLNYFSATEEKISDNISTASEASETTSSRTDM----CFARQHNTSDNNNQCL---LVSNGN--------------------TLLQLNPQKPGAIDNQPLVTQEPVKAAS----LTMEGGRLKQSPHLVEGRDYIMVPEPVWRALYHWYGANLSLPRPVIKNSKTNMPELELFPCYLL-FLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWIYYSENHLSLLDDEDHRLEYLKIKDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNIAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWENHLRRNRSIVVDLFHGQLKSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSELCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPGSPTSASSPVQTDFSTGPSTNGMLSIMMNGDLPRPTLIPNGMPNTVVPCGTENNLANWTLNGHVSLLPDSPFIGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIECTEDRA-CVGN----AYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFLVQKD 1308 HSP 2 Score: 90.5077 bits (223), Expect = 3.259e-14 Identity = 46/103 (44.66%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ IYNLYAI+CH G + GHYVTYA N +NKWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1504 NPIYNLYAISCHSGIMGGGHYVTYAKNP-NNKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1601
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Match: A0A401RZT3 (Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0002065 PE=3 SV=1) HSP 1 Score: 709.909 bits (1831), Expect = 0.000e+0 Identity = 470/1420 (33.10%), Postives = 700/1420 (49.30%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKM-LSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIIT---------------QWLL-----WEQKLKVIPYDH--VYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRK---HQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE--------------DTECSEI-------NKNGSSPSTQENLI--------------------------------KFVIY----HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
MG +SR+ LSY+EA KR+T+ EL RLK AF S +S F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ F+L+ NES S + E+E + A D V++ E + ++ + W+ +N ++WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++E + E+ + D D++ IL + +++++D IW FLNL+ QIC IV GL+P EE II+ W L W Q + + Y+ V I S N N LN +++ + + N + + K +IS+ + + + +Q A ++ R+N NG+ + + +I+ + P +P + + + + LT +GG L +PTL +D+EI+P P+W ++WYGA++++ RP+ T ++ + + F R QT + + + + + PS P KR +AY FS I D+ E LS++L I E+MR+W EN L L+DDED L+DL + + +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT YFI HL E+N R NP G +G +A YG+LI+++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C C SVRFD ++ LSLP+PM EI V + + P +Y + N +D + + +Q+S ++ ILL + + IK F N ++ L+ + + FE T+ S++ N++ S+ + NLI F+ Y HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V+ + CS C W FC GC + + SD VG Y+AIDW ALHL E+ + + ++ +P++L C+ +FT++E L +D LY C KCK + A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P D + F+
Sbjct: 1 MGAKESRIGFLSYDEAVKRVTDIELKRLKDAFKRTSGLSFYMSQQAFVREVLGDGVPPKVAEVIYHSFGG--TSKGLHFNNLIVGLVLLTRGRDEEKAKYIFNLFANESGSYVVREEMETMLRACDGEVQ----LGVKKCFVEGEKVNYEKFKNWLLQNKDAFTFARWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHPSLTEGLFSAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDTDQDGALSRAELEEMVVALLEVWKDNRTDSIPELQMGIADIVNHILKKHDTTKMGHLTLEDYQIWSVQSALASEFLNLLFQICHIVLGLRPATPEEEGQIISGWLERESRHGLQPGHNWFLISVQWWHQWKEYVKYEAKPVIIEPSSITNLMKNQTSLNAADQGEDKPEVQNSFSSADEKCFDNISIASEASESTGTGLYQSSPAGTDMCFARQNNSLDNNGQCF----LSANGNILAQLGPQ-------------------------------KPGAIDNQSLV-------MQGSVKVPTLTLEGGRLKQNPTLVCGKDYEIVPEPVWRALYHWYGANLALPRPVIRNTAS-EEAELELFPRYLLFLRQQTATRTQQSNIWVNMGNVPSPSSPM------------------------------KRVLAYTGCFSRMQTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLALLDDEDHTLEDLKILDEQHLVIEVRNKDMSWPEEMSFIANSSKMDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTRYFISGRHLYELN----RANPLGMKGHMAKCYGDLIQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDFEVAAEAWENHLRRNKSIVVDLFHGQLKSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGSTPVRYGLRLN-MDEKYTGLKKQLSELCGLKSEQILLGEVHGSNIKNFPQDNQKVRLSASGFLCAFEIPVPGSPTSATSPIQTDSSKVPLTNGKHNQSSSANPMKPNLIPNGIPSTVVPTEIEKNLSNGLPNGHLPAASDSPFIGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDSV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVHKDGNTCSWCSWYRFCRGCKI-DCSDERAAVGN----AYIAIDWDPTALHLRYQTSQERICEEHESVEESRRAQAEPINLDSCLRAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPREGFDPSAFL 1309 HSP 2 Score: 89.3521 bits (220), Expect = 6.795e-14 Identity = 46/101 (45.54%), Postives = 60/101 (59.41%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+YNLYAI+CH G L GHYVTYA N + KWY +NDS CKEV EI AY+LFYE+ +N FL + + ++ ESD +KYC++Q
Sbjct: 1507 LYNLYAISCHSGILGGGHYVTYAKNP-NEKWYCYNDSSCKEVRPDEIDTDSAYILFYEQQGVNYAQFLPKTEGKKMADTSS----MDEDFESDYRKYCILQ 1602
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Match: A0A151N605 (Ubiquitin carboxyl-terminal hydrolase 32 isoform D OS=Alligator mississippiensis OX=8496 GN=USP32 PE=3 SV=1) HSP 1 Score: 708.753 bits (1828), Expect = 0.000e+0 Identity = 479/1415 (33.85%), Postives = 711/1415 (50.25%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNG-RFVYCTKIDVSDDIV-----------QNIYPDIISIGGD----HXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE------DTECS-----------------EINKNGSSP----------------STQENL-----------------IKFVI-YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKF-IQRD 4097
MG +SR+ LSY+EA +R+T+ EL RLK AF +S F EV G +P V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ NES S + E+E + + D ++++ E + ++ + W+ N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R +++ + E+ + DK +++ IL++ + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + ++I SW QW ++Y K AN + + + F +N IA S A E + +M G + T+ +SD+I +Y I+ G D ++ N+ S+ + P K I+NQ L+ K AS LT +GG L SP L D+ ++P P+W ++WYGA++S+ RP+ ++ + + + F R Q + + + + + P+ P KR +AY FS I ++ E LS++L I E+MR+W EN+L+L+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L++++W+G+ I PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C C SVRFD ++ LSLP+PM EI V + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + + FE T S I NG P T+ NL I ++I HRK+++ YF++ + LFG P+++ +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + D VG Y+A+DW ALHL E+ + + ++ +P++L C+ +FT++E L +D +Y C KCK A KKLDLW LPPILIIHLKRFQF++ RW KS K V++P N D + F +Q+D
Sbjct: 1 MGAKESRIGFLSYDEALRRVTDVELKRLKDAFKRTCGLSCYMSQQCFIREVLGDGVPPKVAEVIYCSFGG--TAKGLHFNNLIVGLVLLTRGKEEEKAKYIFSLFSNESGSYVVREEMERMLHVVDGKVPD----TLKKCFSEGEKVNYEKFKNWLLHNKDAFTFSRWLLSGGVYV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIHPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRTELKEMVVALLEVWKDNRTDKIPELDKDLSEIVEDILNAHDTTKIGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFLIAVSWWQQW-----------KEYVKYDANL-----------VMIESSFVLNGGKNAVIAASTHA--SEQGEEKMGGLNYFSATEEKISDNISTASEASETTSSNTLYS--ITPGTDMCFARQHNTSDNNNQCLLVSNGNTLLQLNPQKPGAIDNQPLVTQEPVKAAS----LTMEGGRLKQSPHLVEGRDYIMVPEPVWRALYHWYGANLSLPRPVIKNSKTNMPELELFPCYLL-FLRQQPATRTQQSNIWVNMGNVPSPNAPL------------------------------KRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWIYYSENHLSLLDDEDHRLEYLKIKDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELN----RTNPIGMKGHMAKCYGDLVQELWSGTQKNIAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAAEAWENHLRRNRSIVVDLFHGQLKSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLN-MDEKYTGLKKQLSELCGLKPEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPGSPTSASSPVQTDFSTGPSTNGMLSIMMNGDLPRPTLIPNGMPNTVVPCGTENNLANWTLNGHVSLLPDSPFIGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTRKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIECTEDRA-CVGN----AYIAVDWDPTALHLRYQTSQERIVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGEDEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFLVQKD 1316 HSP 2 Score: 90.5077 bits (223), Expect = 3.434e-14 Identity = 46/103 (44.66%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ IYNLYAI+CH G + GHYVTYA N +NKWY +NDS CKE+ EI AY+LFYE+ ++ FL ++ + ++ ESD KKYC++Q
Sbjct: 1512 NPIYNLYAISCHSGIMGGGHYVTYAKNP-NNKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGVDYAQFLPKIDGKKMADTSS----MDEDFESDYKKYCVLQ 1609
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Match: ENSAMXT00000013101.2 (ubiquitin carboxyl-terminal hydrolase 32-like [Source:NCBI gene;Acc:103023192]) HSP 1 Score: 486.108 bits (1250), Expect = 5.300e-145 Identity = 304/833 (36.49%), Postives = 442/833 (53.06%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA----KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKF-----------------------LNPNDRIYLTQTLYAYIFE---------------------DTECSEINK--NGS--SPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIK-PNHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG+L SP L +DFE++P P+W ++WYGA++S+ RP+ TRD+ + + F R+ L ++ P+ NG N + + SM PS KR + Y FS I D+ + LS++L I E+MR+W I+EN LTL+DDED L+ L + + +++E RN D++WPEE++ L + K KG TGLSN+GNTCF NS+IQC+SNT LT+YF+ HL E+N RTNP G +G +A YG+LI+++W+G+ I PLKL+ T+ KYA +F G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG L+S V C C S RFD ++ LSLP+PM + EI+V + P +Y + N +D S + +S ++ +LLV I + IK F +P+ + +T A + T+ S N NG +P T + HRK+++ YF++ + LFG P+++ AE +D+ + V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + N D +G+ +A+DW ALHL +S + ++ +S + ++ A++ +P+ L C+ +FTT+E L ++ LY C KCK + A KKLD+W LPPILI+HLKRFQF++ RW KS K VR+P N D + F+
Sbjct: 540 LTMEGGLLRRSPNLHLGQDFEVVPEPVWRALYHWYGANLSLPRPV-IRTRDLENAELEL------FPRH------------LLFLRQQPPARTPQSNGWINMG---VMNQQKAPQSMKGNMPSPSAPLKRVLVYTGCFSRVGTIKDIHDYLSQRLRIKEEDMRLWLYINENYLTLLDDEDHSLEALKIPDEQYMVIEVRNKDMSWPEEMSFLANSSRINRYKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFLSGSHLYELN----RTNPIGMRGHMAKCYGDLIQELWSGTQKSIAPLKLRWTIAKYAPRFDGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAYEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHVSARFDPFNFLSLPLPMDNSMHLEIIVIRLDGSTPVRYGLRLN-VDEKYSGLKSLLSELCHLKAEQLLLVEIHLSCIKNFPLDHQKVRRAVSGFLCAFEIPLPASPSSNLEITSPKEAGVVSSGTSLRLDIIPKGMPNTVVPCSTDGSPANGLPNGHIMTPPTGPFTGYIIAIHRKMMRAELYFLSSQKNRPSLFGMPLIVPC----TAETRCRDLYNTV---------------WTQVS-RLASPLPPQESSNHAQDCDDSMGYQYPFTLRVVQGDGNSCAWCPWYRFCRGCAIECNDDR-AALGSV----CIAVDWDPTALHLRYQTSQERLVEEHVSVELSRRAQA---EPISLDSCLRAFTTEEELGENELYYCSKCKTHRLATKKLDIWRLPPILIVHLKRFQFVNGRWIKSQKIVRFPRDNFDPSAFL 1316 HSP 2 Score: 249.595 bits (636), Expect = 2.202e-66 Identity = 152/422 (36.02%), Postives = 224/422 (53.08%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFS----LYCNES-------ASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LSY+EA KR+T+ EL RL+ AF +S F EV G +P +S I+ + TL G++F L +G+V+LT+G EEK + +F L+C S A+ ++E + A D S+R+ E + ++ + W+ +N S+WLL+S V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ SI E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG + +I+ + E+ + D + D++ IL + K +++++D IW FLNL+ Q+C IV GL+P EE II WL E + + P D+ ++I+ W QW
Sbjct: 1 MGAKESRIGFLSYDEAIKRVTDVELKRLRDAFRRTCGLSCYMSQQCFYKEVLGDMVPLNISEVIYSSFG--GTLKGLHFNNLIVGLVLLTRGRDEEKARCYFYYIIYLFCIHSFIESGSYATREDIESMLQAVDGEVH----PSLRKCFTEAEKVNYEKFKSWLLQNKDAFTFSRWLLSSGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFIPLVSPPIHPSISEGLFHAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLCQEEIQEMVSALLEVWKDNRTDSIPELESSVSDIVEDILRIHDTTKKGHLTLEDYQIWSVQSVLAAEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPGDYWFLISTQWWQQW 415 HSP 3 Score: 88.1965 bits (217), Expect = 6.823e-17 Identity = 43/103 (41.75%), Postives = 64/103 (62.14%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ +Y+LYAI+CH G L GHYVTYA N ++KWY +NDS CKEV+ +EI AY+LFYE+ ++ F+ + + ++ E+D KKYC++Q
Sbjct: 1483 EPVYDLYAISCHSGILGGGHYVTYAKNP-NDKWYCYNDSSCKEVNPEEIDTDSAYILFYEQQGVDYSLFMPKTDGKKMADTSS----MDEDFETDYKKYCVLQ 1580
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Match: ENSAMXT00000044953.1 (ubiquitin carboxyl-terminal hydrolase 32-like [Source:NCBI gene;Acc:103023192]) HSP 1 Score: 474.937 bits (1221), Expect = 4.216e-141 Identity = 301/846 (35.58%), Postives = 437/846 (51.65%), Query Frame = 3
Query: 1767 LTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA--KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYI--------------FEDTECSEIN-------KNGSSPSTQEN-----------------------------------------LIKFVIYHRKLIQKAN-YFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIK-PNHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHL---SSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG+L SP L +DFE++P P+W ++WYGA++S+ RP+ TRD+ + + F R+ L ++ P+ NG N PSA KR + Y FS I D+ + LS++L I E+MR+W I+EN LTL+DDED L+ L + + +++E RN D++WPEE++ L + K KG TGLSN+GNTCF NS+IQC+SNT LT+YF+ HL E+N RTNP G +G +A YG+LI+++W+G+ I PLKL+ T+ KYA +F G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG L+S V C C S RFD ++ LSLP+PM + EI+V + P +Y + N +D S + +S ++ +LLV I + IK F P D + + + ++ E T E NG+SP ++I + + +A YF++ + LFG P+++ AE +D+ + V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + N D +G+ +A+DW ALHL +S + ++ +S + ++ A++ +P+ L C+ +FTT+E L ++ LY C KCK + A KKLD+W LPPILI+HLKRFQF++ RW KS K VR+P N D + F+
Sbjct: 533 LTMEGGLLRRSPNLHLGQDFEVVPEPVWRALYHWYGANLSLPRPV-IRTRDLENAELEL------FPRH------------LLFLRQQPPARTPQSNGWINMGNMPS--------------PSAPLKRVLVYTGCFSRVGTIKDIHDYLSQRLRIKEEDMRLWLYINENYLTLLDDEDHSLEALKIPDEQYMVIEVRNKDMSWPEEMSFLANSSRINRYKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFLSGSHLYELN----RTNPIGMRGHMAKCYGDLIQELWSGTQKSIAPLKLRWTIAKYAPRFDGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDWEVAYEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHVSARFDPFNFLSLPLPMDNSMHLEIIVIRLDGSTPVRYGLRLN-VDEKYSGLKSLLSELCHLKAEQLLLVEIHLSCIKNF--PLDHQKVRRAVSGFLCAFEIPLPASPSSNLEITSPKEAGVGAFPVVANGTSPPVSSGTSLRLDIIPKGMPNTVVPCSTDGSPANGLPNGHIMTPPTGPFTGYIIAIHRKMMRAELYFLSSQKNRPSLFGMPLIVPC----TAETRCRDLYNTV---------------WTQVS-RLASPLPPQESSNHAQDCDDSMGYQYPFTLRVVQGDGNSCAWCPWYRFCRGCAIECNDDR-AALGSV----CIAVDWDPTALHLRYQTSQERLVEEHVSVELSRRAQA---EPISLDSCLRAFTTEEELGENELYYCSKCKTHRLATKKLDIWRLPPILIVHLKRFQFVNGRWIKSQKIVRFPRDNFDPSAFL 1309 HSP 2 Score: 261.536 bits (667), Expect = 4.350e-70 Identity = 152/411 (36.98%), Postives = 224/411 (54.50%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LSY+EA KR+T+ EL RL+ AF +S F EV G +P +S I+ + TL G++F L +G+V+LT+G EEK ++ FSL+ +ES S E I + + + S+R+ E + ++ + W+ +N S+WLL+S V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ SI E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG + +I+ + E+ + D + D++ IL + K +++++D IW FLNL+ Q+C IV GL+P EE II WL E + + P D+ ++I+ W QW
Sbjct: 1 MGAKESRIGFLSYDEAIKRVTDVELKRLRDAFRRTCGLSCYMSQQCFYKEVLGDMVPLNISEVIYSSFG--GTLKGLHFNNLIVGLVLLTRGRDEEKARYIFSLFASESGSYATREDIE-SMLQAVDGEVHPSLRKCFTEAEKVNYEKFKSWLLQNKDAFTFSRWLLSSGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFIPLVSPPIHPSISEGLFHAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLCQEEIQEMVSALLEVWKDNRTDSIPELESSVSDIVEDILRIHDTTKKGHLTLEDYQIWSVQSVLAAEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPGDYWFLISTQWWQQW 407 HSP 3 Score: 88.1965 bits (217), Expect = 5.614e-17 Identity = 43/103 (41.75%), Postives = 64/103 (62.14%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ +Y+LYAI+CH G L GHYVTYA N ++KWY +NDS CKEV+ +EI AY+LFYE+ ++ F+ + + ++ E+D KKYC++Q
Sbjct: 1479 EPVYDLYAISCHSGILGGGHYVTYAKNP-NDKWYCYNDSSCKEVNPEEIDTDSAYILFYEQQGVDYSLFMPKTDGKKMADTSS----MDEDFETDYKKYCVLQ 1576
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Match: usp32 (ubiquitin specific peptidase 32 [Source:ZFIN;Acc:ZDB-GENE-100210-5]) HSP 1 Score: 441.425 bits (1134), Expect = 1.531e-129 Identity = 284/832 (34.13%), Postives = 407/832 (48.92%), Query Frame = 3
Query: 1767 LTEDGGVLSPTLRF--NEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSA--KRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE-DTECSEINKNGSSPSTQEN--------------------------------------------------LIKFVI-YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L L DFE +P P+W ++WYGA++S+ RP+ KS V + F RY LF+ + P + N N P+A KR +AY FS + I D+ E LS++L I E+MR+W EN LTL+DDED L+ L +++ +++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N AL +++ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C C S RFD ++ LSLP+PM + EI V + + P +Y + N +D + + +Q+S ++P ILL + + IK F N ++ L+ + FE S + +P+ +E+ I ++I HRK+++ YF++ + LFG P+++ + +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + CS C W FC GC + N D +G Y+A+DW ALHL E+ + ++ +P+ L C+++FT++E L +D LY C KCK + A KKLDLW LPPILI+HLKRFQF++ RW KS K V++P D + F+
Sbjct: 514 LTMEGGRLKRALHLMAGRDFETVPEPVWRALYHWYGANLSLPRPV-------IKSVVTEEAELELFPRY-----------LLFLRQQPATRTPQS-NIWVNMGNVPS--------------PNAPLKRVLAYTGCFSRMSTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFISGRHLYELNRL-----------YTALE-------------------QIRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDAEVATEAWENHLRRNRSIVVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLN-MDEKYTGLKKQLSELCGLKPEQILLAEVHSSNIKTFPQDNQKVRLSANGFLCAFEIPVPGSPTSVTSPTPTGEEDNLSLLSVLFNLGSDKNLANGMPSTVVPSASEKSLTNGIPNGHITPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTSKKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCSWCPWYRFCRGCTVECNEDR-AALGN----AYIAVDWDPTALHLRYQTSQERIVEEHASVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPILIVHLKRFQFVNGRWIKSQKIVKFPRGGFDPSAFL 1255 HSP 2 Score: 236.498 bits (602), Expect = 2.077e-62 Identity = 147/415 (35.42%), Postives = 226/415 (54.46%), Query Frame = 3
Query: 159 ITNKELNRLKRAFNEYSQ-KKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGI---ELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDI-EYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR-LNNSEKEDYNKDS 1382
IT+ EL RLK AF S ++ F EV G +PS V+ I+ + T G++F L +G+V+LT+G EEK K+ FSL+ N+S S + E+E + + D + S+++ E + ++ + W+ +N S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++ E + + E K N + + S + D++ IL + + +++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + P + ++I+ W W + + ++N+ K NK S
Sbjct: 24 ITDVELKRLKDAFKRTSGLSAYMTQQCFFKEVLGDGVPSKVAEVIYNSFG--GTPKGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFANDSGSYVVRDEVENMLRSVDG----EVPPSLKKCFLEGEKVNYERFKGWLLQNKEAFTFSRWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEVHEMVVALLEVWKDNRTDTLPELHSNV-SDIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPAQNWFLISMQWWQLWKDYVKYVSNTVKLRNNKQS 430 HSP 3 Score: 88.9669 bits (219), Expect = 3.496e-17 Identity = 45/103 (43.69%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+ IY+LYAI+CH G + GHYVTYA N ++KWY +NDS CKEV +EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1435 EPIYDLYAISCHSGIMGGGHYVTYAKNP-NDKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSRFLPRTDGKKMADTTS----MDEDFESDYKKYCVLQ 1532
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Match: usp4 (ubiquitin specific peptidase 4 [Source:NCBI gene;Acc:103035454]) HSP 1 Score: 305.834 bits (782), Expect = 4.058e-86 Identity = 217/719 (30.18%), Postives = 337/719 (46.87%), Query Frame = 3
Query: 2430 GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFE-ILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRI-YLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHS--RTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVL---------------AQVNSNIKHCSICHWSN-FCNG---------CPLPNNSDA---------------------------------YNTVGTFN--------------DEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKT-VRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
G+ GLSN+GNTCF NSA+QC+SN+ LTEYF+ D + EIN R NP G +G +A Y +L+K +W ++ + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y L+D+ GR D+ VA+EAW H N SI+VD+FHGL KS + C C SV FD + L+LP+PM+ ++ E ILV + P KY V+ I T + +C +S+ V ++++ + +++ K + + ++ +I+E E + +Y R+ M HS + T LFG P+++TV + ++++ T + ++V + + S V + ++ +H++DC H + N + H C+ N C+G P P +S + NT GT N +AIDW + L + + + + + A+ + L +CIE FTT E L + + C CKK QRA KK DLW+LP IL++HLKRF + ++ W+ T V +P+R+L++++F+ + P +Y+L A++ H G + GHY YA NK KWY F+DS E +I AY+LFY+R
Sbjct: 318 GLCGLSNLGNTCFMNSALQCLSNSPPLTEYFLEDQYEAEIN----RENPLGMRGEIAEAYADLVKQMWLSRSSYVAPRTFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLALRDAEGRPDEIVAKEAWTNHRLRNDSIIVDIFHGLFKSTLVCPECSKVSVTFDPFCYLTLPLPMKKDRTMEVILVCSDPLSRPTKYRVVVPKI-GTVADLCSALSKLSGVPAENMVVADVYNHRFHKIYRKEEGLNHIVDKDIIFIYEVLE------------EDSERMNLPVYFRE------RHMKHSGGSSGTMLFGQPLLVTVPRHNLSQDT---LYERVLERIGRYVKRSQ---GTVVDSRASASATSCNSSHSVDCGVSASNHSAGVSSCSSPMSDGASCSAGSTNGSSSHSETCNGPNGVCDGEVEAMDHQDSPDPESSQSETVDGEDEGSEPENGPVASVGKASRPKLFTFSMVNTYGTANISPLPCEGNILKLTPHSTIAIDWEADSKRLCYDDQEAEAYEKHESMMQAQKKKTT-VALKECIELFTTMETLGEHDPWYCPTCKKHQRATKKFDLWSLPRILVVHLKRFSY-NRCWRDKLDTIVDFPIRDLNMSEFVC-----------------DPKAAP-----YVYDLVAVSNHYGGMGGGHYTAYAKNKADGKWYYFDDSSVSSASEDQIVTKAAYVLFYQR 982
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Match: usp4 (ubiquitin specific peptidase 4 [Source:NCBI gene;Acc:103035454]) HSP 1 Score: 296.975 bits (759), Expect = 2.961e-84 Identity = 206/644 (31.99%), Postives = 313/644 (48.60%), Query Frame = 3
Query: 2430 GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFE-ILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKT-VRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
G+ GLSN+GNTCF NSA+QC+SN+ LTEYF+ D + EIN R NP G +G +A Y +L+K +W ++ + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y L+D+ GR D+ VA+EAW H N SI+VD+FHGL KS + C C SV FD + L+LP+PM+ ++ E ILV + P KY V+ I T + +C +S+ V ++++ + +++ K IY + +I + K +I+ + N +HS T G V ++ + +QD D + V D+ S +N P+ + ++ F + VN+ N PLP + + +AIDW + L + + + + + A+ + L +CIE FTT E L + + C CKK QRA KK DLW+LP IL++HLKRF + ++ W+ T V +P+R+L++++F+ + P +Y+L A++ H G + GHY YA NK KWY F+DS E +I AY+LFY+R
Sbjct: 280 GLCGLSNLGNTCFMNSALQCLSNSPPLTEYFLEDQYEAEIN----RENPLGMRGEIAEAYADLVKQMWLSRSSYVAPRTFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLALRDAEGRPDEIVAKEAWTNHRLRNDSIIVDIFHGLFKSTLVCPECSKVSVTFDPFCYLTLPLPMKKDRTMEVILVCSDPLSRPTKYRVVVPKI-GTVADLCSALSKLSGVPAENMVVADVYNHRFHK-------IYRKEEGLNHIVD---------------------KDIIF---IAGSTNGSSSHSETCNGPNG------VCDGEVEAMDHQDSPDPESSQSET------VDGEDEGSEPENGPVASVG--------KASRPKLFTFSMVNT----------YGTANISPLPCEGN----ILKLTPHSTIAIDWEADSKRLCYDDQEAEAYEKHESMMQAQKKKTT-VALKECIELFTTMETLGEHDPWYCPTCKKHQRATKKFDLWSLPRILVVHLKRFSY-NRCWRDKLDTIVDFPIRDLNMSEFVC-----------------DPKAAP-----YVYDLVAVSNHYGGMGGGHYTAYAKNKADGKWYYFDDSSVSSASEDQIVTKAAYVLFYQR 828
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Match: usp32 (ubiquitin specific peptidase 32 [Source:ZFIN;Acc:ZDB-GENE-100210-5]) HSP 1 Score: 437.958 bits (1125), Expect = 9.989e-131 Identity = 272/796 (34.17%), Postives = 414/796 (52.01%), Query Frame = 3
Query: 1749 ASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRK-ASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE------DTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTE----------LFGYPIVLTVRKDDIAEITNQDIQDKV-------------AHILNNQIF-------------HSHVYPA--DQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILI 3989
A LT +GG L SP L + +D+EI+P P+W +WYG +I + R + + +++E+ + +++++H P PT+ + G + + KR +AY A FS + + E L+++L + +E++R+W + DENNLTL+DD+++ ++ L + + +IL+E RN D++WPEE++ L K+L+ K KG TGLSN+GNTCF NS+IQC+SNT LT+YF+ +HL E+N RTNP G +G +A YG+LI ++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C +C SVRFD ++ LSLP+PM + EI V ++P +Y + D S + +Q+S S++P ILL + + I+ F + N ++ T Y FE D + +P F++ + L+ AH T L + + R + + + V +H +F H H+Y QV+ + SP+P + NHA DC DS+ ++PF L+ V + C+ C W FC GC + N+ + VG+ Y+AIDW ALHL E+ L + ++ +P+ L C+++FT +E L +D LY C KC+ + A KKLD+W LPP L+
Sbjct: 514 AVKVPTLTAEGGRLKCSPPLMWGQDYEIVPDPVWRSLSHWYGYNIVLPRTVIPQVNG-------------------RPLELELYPVCIYLLRHQQP--PTSARLAAAAAAAGGVASAPA---------PPKRYLAYTACFSTMLSVRQIHEFLAQRLRLRQEDLRLWLLKDENNLTLLDDDEQTIKSLRIEDNQQILIEVRNKDMSWPEEMSSLANRKNLERSKLVPTEKGATGLSNLGNTCFMNSSIQCVSNTRQLTQYFLSGMHLYELN----RTNPLGMKGHMAKCYGDLINELWSGTQKNVAPLKLRWTIGKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDHEVATEAWENHLLRNKSIVVDLFHGQLKSQVRCKMCDHVSVRFDPFNFLSLPLPMDSSMHVEITVIRLDGSVPVRYGLRLGVEDKYSG-LKQQLSELCSLQPQQILLAEVFSSNIRNFPDDNQKVRSGATSYLCAFEIPVLSGDPASTSPTDAHFTPIGGPRHFTFIVLNDNLLPPCFAPTAHISTRGAGGLAKSASNGLTNGHLAQSCRPEQLGGVQPSGYAIAVHRKMMRMEVYFLSSHKNRPSLFGMPLIVPCAAGTQHCHLYDLVWGQVA-RLVSPLPPQEASNHAQDCDDSLGYQYPFTLSAVQKDGVTCAWCPWYRFCRGCKIECNTVVF--VGSSG--VYIAIDWDPTALHLRYQTAQERVCLEHESVGESRRAQTEPISLNSCLQAFTKEEELGEDELYYCSKCRAHRLASKKLDIWRLPPSLV 1268 HSP 2 Score: 215.698 bits (548), Expect = 7.180e-57 Identity = 145/407 (35.63%), Postives = 215/407 (52.83%), Query Frame = 3
Query: 159 ITNKELNRLKRAFNEYSQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTH-LDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCC-KIFNKTMDGKFT-------RGDIEY--IFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR 1343
+T+ EL RL+ AF ++ F EV G +P V+ I+ + ++ G++FKEL +G+V+LT+G EEK K+ FSL NES S + E + ++ +VRQ E+ ++ + +W+ N +S+WLL S V LTDDS YQ +AGVTH ++E +II+LEK Y L + + F+L F LV PPLP S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F++ DG + I Y IF + E ++NE ++ + ++ +IL + + +S++D IW FL L+ Q+C IV GL+P + EE II WL E + P Y+I+ W QW + R
Sbjct: 4 VTDDELKRLRDAFKRSCGLAYMNQQCFVREVLGEGVPPKVAEVIYNSFGGSS--KGLSFKELVVGLVLLTRGRDEEKIKYIFSLCANESGSYVSREGLEGLLQACETQPLAETVRQCFRESERANYERFKQWLLANKELLILSRWLLASTVSV-TLTDDSDTPTFYQTLAGVTHSVEESDIIDLEKRYWLLKAQSRTGHFDLETFAPLVSPPLPPSLTEGIFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCSFKVFDRDRDGLLSNLEDSVCENAIFYFPIFAL-RETQHNEEMNMETAA------VVNAILQMHDTNQEKSLSLEDYQIWSVTSTLAHEFLTLLFQVCHIVLGLRPASPGEEGQIIRGWLEREHRQGFRPGQLWYLISMQWWQQWKDYVR 400
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Match: usp32 (ubiquitin specific peptidase 32 [Source:ZFIN;Acc:ZDB-GENE-100210-5]) HSP 1 Score: 356.295 bits (913), Expect = 1.847e-102 Identity = 197/501 (39.32%), Postives = 295/501 (58.88%), Query Frame = 3
Query: 1749 ASNFTILTEDGGVL--SPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRK-ASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE 3242
A LT +GG L SP L + +D+EI+P P+W +WYG +I + R + + +++E+ + +++++H P PT+ + G + + KR +AY A FS + + E L+++L + +E++R+W + DENNLTL+DD+++ ++ L + + +IL+E RN D++WPEE++ L K+L+ K KG TGLSN+GNTCF NS+IQC+SNT LT+YF+ +HL E+N RTNP G +G +A YG+LI ++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SIVVDLFHG LKS V C +C SVRFD ++ LSLP+PM + EI V ++P +Y + D S + +Q+S S++P ILL + + I+ F + N ++ T Y FE
Sbjct: 488 AVKVPTLTAEGGRLKCSPPLMWGQDYEIVPDPVWRSLSHWYGYNIVLPRTVIPQVNG-------------------RPLELELYPVCIYLLRHQQP--PTSARLAAAAAAAGGVASAPA---------PPKRYLAYTACFSTMLSVRQIHEFLAQRLRLRQEDLRLWLLKDENNLTLLDDDEQTIKSLRIEDNQQILIEVRNKDMSWPEEMSSLANRKNLERSKLVPTEKGATGLSNLGNTCFMNSSIQCVSNTRQLTQYFLSGMHLYELN----RTNPLGMKGHMAKCYGDLINELWSGTQKNVAPLKLRWTIGKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKP-YVELKDSDGRPDHEVATEAWENHLLRNKSIVVDLFHGQLKSQVRCKMCDHVSVRFDPFNFLSLPLPMDSSMHVEITVIRLDGSVPVRYGLRLGVEDKYSG-LKQQLSELCSLQPQQILLAEVFSSNIRNFPDDNQKVRSGATSYLCAFE 952 HSP 2 Score: 205.682 bits (522), Expect = 1.014e-53 Identity = 138/381 (36.22%), Postives = 203/381 (53.28%), Query Frame = 3
Query: 237 FCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTH-LDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCC-KIFNKTMDGKFT-------RGDIEY--IFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFR 1343
F EV G +P V+ I+ + ++ G++FKEL +G+V+LT+G EEK K+ FSL NES S + E + ++ +VRQ E+ ++ + +W+ N +S+WLL S V LTDDS YQ +AGVTH ++E +II+LEK Y L + + F+L F LV PPLP S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F++ DG + I Y IF + E ++NE ++ + ++ +IL + + +S++D IW FL L+ Q+C IV GL+P + EE II WL E + P Y+I+ W QW + R
Sbjct: 6 FVREVLGEGVPPKVAEVIYNSFGGSS--KGLSFKELVVGLVLLTRGRDEEKIKYIFSLCANESGSYVSREGLEGLLQACETQPLAETVRQCFRESERANYERFKQWLLANKELLILSRWLLASTVSV-TLTDDSDTPTFYQTLAGVTHSVEESDIIDLEKRYWLLKAQSRTGHFDLETFAPLVSPPLPPSLTEGIFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCSFKVFDRDRDGLLSNLEDSVCENAIFYFPIFAL-RETQHNEEMNMETAA------VVNAILQMHDTNQEKSLSLEDYQIWSVTSTLAHEFLTLLFQVCHIVLGLRPASPGEEGQIIRGWLEREHRQGFRPGQLWYLISMQWWQQWKDYVR 376 HSP 3 Score: 119.783 bits (299), Expect = 2.511e-27 Identity = 74/230 (32.17%), Postives = 115/230 (50.00%), Query Frame = 3
Query: 3312 VIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPA--DQVSVKQNSPIPIIKP-NHALDCD-SVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILI 3989
+ HRK+++ YF++ + LFG P+++ A T H H+Y QV+ + SP+P + NHA DCD S+ ++PF L+ V + C+ C W FC GC + N+ + VG+ Y+AIDW ALHL E+ L + ++ +P+ L C+++FT +E L +D LY C KC+ + A KKLD+W LPP L+
Sbjct: 1035 IAVHRKMMRMEVYFLSSHKNRPSLFGMPLIVPC-----AAGTQ----------------HCHLYDLVWGQVA-RLVSPLPPQEASNHAQDCDDSLGYQYPFTLSAVQKDGVTCAWCPWYRFCRGCKIECNTVVF--VGSSG--VYIAIDWDPTALHLRYQTAQERVCLEHESVGESRRAQTEPISLNSCLQAFTKEEELGEDELYYCSKCRAHRLASKKLDIWRLPPSLV 1238
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Match: usp4 (ubiquitin specific peptidase 4 (proto-oncogene) [Source:ZFIN;Acc:ZDB-GENE-041008-187]) HSP 1 Score: 244.588 bits (623), Expect = 7.564e-67 Identity = 154/447 (34.45%), Postives = 234/447 (52.35%), Query Frame = 3
Query: 2160 FSHRTRITDLIELLSKKLNINRE-EMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEE--LNQLVTLKSLQ-----------------------NRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHR-KLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
FS I + + + K +I E E R+W N ++ +K LQD+ L +++EQ+N D TWP + + T KS + R + + G+ GLSN+GNTCF NSA+QC+SNT LT YF+ D +L E+N R NP G +G +A Y +LIK +W+GS + VP K + ++A QFSG QQ D+QELL+ LLD LHEDLNRI KP Y ELKD+ GR D VAQEAW H N SI+VD+FHGL KS + C C SV FD + L+LP+P++ + E+ LV++ + P Y V+ + S S +C + V ++++ + +++ K N+ + +D E+ ++P + + +Y R + ++ Y + S T LFG P++++V +
Sbjct: 155 FSKADTIDTIEKEMRKLFHIPEEKETRLWNKYMTNTYEPLNKPEKTLQDVGLFQGQVLVIEQKNEDGTWPRQSPTTKYGTYKSFKSILKVNSSSSSSNNYNSSYNSYSGRGGTSTPGLCGLSNLGNTCFMNSALQCLSNTPPLTTYFLEDAYLKELN----RDNPLGMRGEIACAYADLIKQMWSGSYSSTVPRSFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRIRKKP-YVELKDAEGRDDKVVAQEAWDNHRKRNDSIIVDIFHGLFKSTLICPDCAKVSVTFDPFCYLTLPLPIKTERAVEVTLVWQDPKSKPTMYRVVVPKMGSVSD-LCAALGLLADVPKENMVVTDVYNHRFHKIFTMNEPLNGINDR-----DDIFVFEVADEDTAP----DHVHVAVYMRERKTSRSGY--SSSCASTSLFGLPLLVSVPR 584 HSP 2 Score: 117.087 bits (292), Expect = 1.511e-26 Identity = 73/209 (34.93%), Postives = 105/209 (50.24%), Query Frame = 3
Query: 3732 YLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKT-VRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
YLA++W N +K+F D P+ L +CIE FTT+E L + C CKK ++A KK DLW LP +L++HLKRF + +K W+ T V +P+R+LD+++F+ TQ + Y+L A++ H G + GHY YA NK N WY F+DS E +I AY+LFY+R
Sbjct: 736 YLALEWDSETRARFYNNNVDKEFEKHDSMLAQPLKKKNPVKLKECIELFTTKEELAATDPWYCPNCKKHKQATKKFDLWALPKVLVVHLKRFSY-NKYWRDKLDTLVDFPIRDLDMSEFVI-------------------GTQ---DVPCTYDLIAVSNHYGGMGGGHYTAYAKNKGENSWYYFDDSSVSSSAEDQIVSKAAYVLFYQR 921
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001826.1 (pep scaffold:Pmarinus_7.0:GL478831:2528:40053:1 gene:ENSPMAG00000001614.1 transcript:ENSPMAT00000001826.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 228.024 bits (580), Expect = 1.337e-62 Identity = 147/472 (31.14%), Postives = 228/472 (48.31%), Query Frame = 3
Query: 2157 SFSHRTRITDLIELLSKKLN-----INREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEEL-------------------NQLVTLKSLQNR---------------------------KASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
SF+ D I+ + K++ +E R+W N ++ D +QD L +++EQ+N D TWP + T S R + + G+ GLSN+GNTCF NSAIQC+SNT LTEYF+ + ++ E+NN NP G +G +A Y +L K IW+G + + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNRI KP Y ELKD+ GR D+ VA+EAW+ H N SI+VD+FHGL KS + C C SV FD + L+LP+PM+ ++ E+ L+ + P +Y V S +C +S+ V + +++ + +++ K ++++ I E + G E ++ +YHR+ + N + + + LFG P+ ++V +
Sbjct: 32 SFTRHFSKADTIDTIEKEMKKLFEIPEDKETRLWNKYTSNTYEPLNKLDSSIQDAGLCQGQVLIIEQKNEDGTWPRQFISNNFPTSPGLSSASSSLAIGSSTYSSGYGRTNRFNPFNSSSGYNSYGYSSYDYREPGRGTSQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFVNNKYVSELNN----ENPLGMKGEIAKAYADLTKQIWSGRYSYVTPRAFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRILNKP-YVELKDAEGRPDEVVAREAWENHQKRNNSIIVDIFHGLFKSTLVCPECSKVSVTFDPFCYLTLPLPMKKERNMEVFLICMDPRSKPTQYKVTVPKSGQISD-LCVALSKLSGVPAHKMVVADVYNHRFHKIFQMDEKL-------NNILERDDIFVYEVAGGKQDDPEQVV-LPVYHREKRIRHNSYNCNLVSTGMLFGQPLFISVPR 489 HSP 2 Score: 71.2478 bits (173), Expect = 1.230e-12 Identity = 37/97 (38.14%), Postives = 55/97 (56.70%), Query Frame = 3
Query: 3723 DEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQF 4013
D+ Y+A+DW + K + F ++ ++ T + L CIE FTT+E L D + C CK+ Q+A KK DLW+LP IL++HLKRF +
Sbjct: 643 DQPYIALDWDPELKRRYYDEKAAEDF-ERHESMQSQPTRKHSVRLRDCIELFTTKERLGVDDPWYCPSCKRHQQATKKFDLWSLPHILVVHLKRFSY 738
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003329.1 (pep scaffold:Pmarinus_7.0:GL476462:53018:71827:1 gene:ENSPMAG00000003036.1 transcript:ENSPMAT00000003329.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 150.984 bits (380), Expect = 1.111e-37 Identity = 91/268 (33.96%), Postives = 134/268 (50.00%), Query Frame = 3
Query: 2430 GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEV--------AQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICE------QVSRQLSVEPNSILLVSIEDNKIKKFLN 3191
G TGL N+GNTCF NS IQ +SNT L +Y D NYS N G G +A+ + +++ +W G+ P KLK + A QFSG QHDAQE L+ LLD LHEDLN P+ ++ + +G D+V A+ AW H A N S +V LFHGL KS + C +C S+ FD + L +P+P Q +L L P + V + + + + + + V P+++ LV + + ++F+
Sbjct: 2 GFTGLVNLGNTCFMNSVIQALSNTRELRDYLHADSAFKADINYS---NVLGTGGRLAVSFAIILRTLWKGTHYAFSPSKLKEIVSNKASQFSGFSQHDAQEFLAFLLDGLHEDLNLAANTPTPQQYPEPHGSLSDQVPRPSHNHMAERAWARHKAWNDSFIVHLFHGLYKSKLVCPVCGHVSITFDPFLYLPVPLP----QRQRVLPVYFLPREPYRRPVKVPILRGGQRRMGKIHANVILLGKMMKVHPDNLKLVQVNKARFERFVQ 262 HSP 2 Score: 100.523 bits (249), Expect = 1.168e-21 Identity = 61/183 (33.33%), Postives = 88/183 (48.09%), Query Frame = 3
Query: 3831 STANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRW-QKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTY-----ALNKESN--KWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
S P L C++ FT E L + + C +C++P+ A K+L LW LPP+L++ LKRF F W +K V +P+R LDL++F + + A+Y+LYA H G L GHY Y A N + + W F+DS V+E ++ AYLLFY R
Sbjct: 474 SGKKGPTSLHHCLKLFTKPETLTPEEAWLCPQCEQPREALKQLMLWRLPPVLVVQLKRFSFCSSVWREKISNLVTFPIRGLDLSEFC--------------VEAASGGAGGDAGGAAVYDLYAAINHYGGLIGGHYTAYARLPCARNSQCSDMGWRWFDDSTVTMVNESQVMSRHAYLLFYRR 642
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Match: UBP12 (Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm [Source:SGD;Acc:S000003733]) HSP 1 Score: 142.124 bits (357), Expect = 2.146e-34 Identity = 106/373 (28.42%), Postives = 173/373 (46.38%), Query Frame = 3
Query: 2412 KASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLI------VPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDD--------EVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIP----------------KKYCVLFNTIDSTSSFICEQVSRQL-----SVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDI 3425
K + G TGL N+GNTC+ NSA+QC+ + L +YF+ D + EIN NP G G VA + +L++ ++ +++ P K T+ + FSG Q D+QE L+ LLD+LHEDLNRI K Y E K S DD ++A + W+ H+ N S++ DLF G+ KS + C C+ S+ FD Y+ ++LP+P+ D I +F +++ P K Y + +D + F CE S Q+ S E N+ L +++ + P D + + P T +N + + + ++ + M LFG P +T+++D++
Sbjct: 356 KLEPASGTTGLVNLGNTCYMNSALQCLVHIPQLRDYFLYDGYEDEIN----EENPLGYHGYVARAFSDLVQKLFQNRMSIMQRNAAFPPSMFKSTIGHFNSMFSGYMQQDSQEFLAFLLDSLHEDLNRI-IKKEYTE-KPSLSPGDDVNDWNVVKKLADDTWEMHLKRNCSVITDLFVGMYKSTLYCPECQNVSITFDPYNDVTLPLPVDTVWDKTIKIFP-MNSPPLLLEVELSKSSTYMDLKNYVGKMSGLDPNTLFGCEIFSNQIYVNYESTESNAQFLT------LQELIKPADDVIFYEL--------------------PVTNDNEVIVPVLNTRIEKGYKNAM--------LFGVPFFITLKEDEL 687 HSP 2 Score: 96.6709 bits (239), Expect = 1.862e-20 Identity = 62/201 (30.85%), Postives = 96/201 (47.76%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTF----LQQVLSNVHNTVPQAVEIISDE 4445
L C++ F+ E+L + C CK+ ++A K++ LW P IL+IHLKRF+ K TV +P+ +LDL++++ D IY+LYA++ H G L GHY Y N NKWY F+DS E + AYLLFY R + + + LQ+++ + + ++ I DE
Sbjct: 964 LDDCLQLFSKPEILGLTDSWYCPTCKEHRQATKQIQLWNTPDILLIHLKRFESQRSFSDKIDATVNFPITDLDLSRYV---------------------VYKDDPRGLIYDLYAVDNHYGGLGGGHYTAYVKNFADNKWYYFDDSRVTETAPENSIAGSAYLLFYIRRHKDGNGLGSSKLQEIIQKSRHGYDERIKKIYDE 1143
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Match: DOA4 (Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication [Source:SGD;Acc:S000002476]) HSP 1 Score: 116.316 bits (290), Expect = 1.332e-26 Identity = 69/198 (34.85%), Postives = 104/198 (52.53%), Query Frame = 3
Query: 2433 VTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEIN-NYSLRTNPQGCQGIVALRYGELI----KDIWTGSTNL-IVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYREL-KDSNGRHDDEVAQEA----WQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
GL N+GN+C+ N IQCI T+ LT+ F+ D + IN N L G +GI+A + L+ K+ GS + I P+K K+ F Q D QE LLD LHEDLN+ + P +EL +++ R + + A W+ + T+FS++VDLF G S + C +C S + +++LS+PIP
Sbjct: 561 AVGLENLGNSCYMNCIIQCILGTHELTQIFLDDSYAKHININSKL-----GSKGILAKYFARLVHMMYKEQVDGSKKISISPIKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEARREKLSLRIASSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIP 753 HSP 2 Score: 78.1814 bits (191), Expect = 8.333e-15 Identity = 58/176 (32.95%), Postives = 76/176 (43.18%), Query Frame = 3
Query: 3864 CIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAI----------YNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITM-SCAYLLFYERA 4358
C FT E L+ D + C C+K Q + K+L + LP LI+HLKRF D K++ V YP LDL F D + PG +D + Y LY + CH G+L GHY Y W F+D+ K V K + S AY+LFY R
Sbjct: 766 CFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRF---DNLLNKNNDFVIYPFL-LDLTPFWANDFDGVF--------------PPGVNDDELPIRGQIPPFKYELYGVACHFGTLYGGHYTAYVKKGLKKGWLYFDDTKYKPVKNKADAINSNAYVLFYHRV 923
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Match: UBP5 (Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication [Source:SGD;Acc:S000000946]) HSP 1 Score: 105.145 bits (261), Expect = 3.389e-23 Identity = 60/200 (30.00%), Postives = 102/200 (51.00%), Query Frame = 3
Query: 2433 VTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDI-----WTGSTNLIVP---LKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEA-----WQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
+ GL NIGN C+ N +QC+ T+ L F+ + +L IN S R G +G++A + L+ ++ +T +P ++ K +S + Q D QE LLD LHEDLN+ +K ++L D R ++++ W+ + T+FS ++DLF G S + C +C+ S + +S+LS+P+P
Sbjct: 445 IVGLENIGNCCYMNCILQCLVGTHDLVRMFLDNTYLNFINFDSSR----GSKGLLAKNFAILVNNMHRHGAFTPPNVRTIPVQTIQFKKICGHINPMYSDSMQQDCQEFCQFLLDGLHEDLNQNGSKKHLKQLSDEEERMREKMSIRKASALEWERFLLTDFSAIIDLFQGQYASRLQCQVCEHTSTTYQTFSVLSVPVP 640 HSP 2 Score: 92.0485 bits (227), Expect = 3.591e-19 Identity = 61/171 (35.67%), Postives = 83/171 (48.54%), Query Frame = 3
Query: 3852 DLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQP--GDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVD-EKEITMSCAYLLFYER 4355
+++ C FT E L D + C KC K Q + K+L + LP LII+LKRF D + K++ V+YP +LDL + RD N+ N P G Y LY + CH GSL GHY +Y WY F+DS+ + + E AY+LFYER
Sbjct: 646 NILDCFREFTKCERLGVDEQWSCPKCLKKQPSTKQLKITRLPKKLIINLKRF---DNQMNKNNVFVQYPY-SLDLTPYWARDFNHEAI---------VNEDIPTRGQVPPFRYRLYGVACHSGSLYGGHYTSYVYKGPKKGWYFFDDSLYRPITFSTEFITPSAYVLFYER 803
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Match: UBP8 (Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B [Source:SGD;Acc:S000004836]) HSP 1 Score: 78.5666 bits (192), Expect = 2.943e-15 Identity = 50/172 (29.07%), Postives = 84/172 (48.84%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQ-FLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLN 4367
L +C++SF +E L KD Y C +C Q A K+L + LP +L++ LKRF+ L+ +K + +P L++ + + + ++ G D IY L I H G++ GHY+ + +W+ FNDS+ + ++E+ AYLLFY +N
Sbjct: 316 LYECLDSFHKKEQL-KDFNYHCGECNSTQDAIKQLGIHKLPSVLVLQLKRFEHLLNGSNRKLDDFIEFPTY-LNMKNYCS-------------TKEKDKHSENGKVPDIIYELIGIVSHKGTVNEGHYIAFC-KISGGQWFKFNDSMVSSISQEEVLKEQAYLLFYTIRQVN 471 HSP 2 Score: 48.9062 bits (115), Expect = 5.403e-6 Identity = 55/198 (27.78%), Postives = 88/198 (44.44%), Query Frame = 3
Query: 2430 GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIR-DVHLCEINNYSLRTNPQGC-----QGIVALRYGEL-IKDIWTGSTNL---IVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNS-VRFDAYSMLSLPI 2990
G++GL N+G+TCF +S +QC+ I YFIR + NN +R+ P C IV YG L K + ST+ + L K + +G Q DA E +++ +H+ + P+ +E+ +N + + IV +F G L+S++ C C+ NS D + LSL I
Sbjct: 135 GLSGLINMGSTCFMSSILQCL----IHNPYFIRHSMSQIHSNNCKVRS-PDKCFSCALDKIVHELYGALNTKQASSSSTSTNRQTGFIYLLTCAWKINQNLAGYSQQDAHEFWQFIINQIHQSY--VLDLPNAKEVSRANNKQCE---------------CIVHTVFEGSLESSIVCPGCQNNSKTTIDPFLDLSLDI 310
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Match: UBP11 (Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication [Source:SGD;Acc:S000001806]) HSP 1 Score: 65.855 bits (159), Expect = 3.634e-11 Identity = 51/193 (26.42%), Postives = 84/193 (43.52%), Query Frame = 3
Query: 2430 GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLK-LKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYREL--KDSNGRHDDEVAQEAWQAH--IATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
+TGL N NTC+ NS IQC+ T + + F+ + +N Q + I L K ++ I+P + LKM + Q D QE L ++L +HE+L+ + Y +L D+N + E W I S + ++ G L++ + C C +S + + +LSL IP
Sbjct: 296 SITGLQNPCNTCYINSIIQCLFGTTLFRDLFLTKKYRLFLNTNKYPKEVQLSRSIYV-----LFKKMYLNGGRAIIPNRFLKMCKKLRPDLNIPDDQQDTQEFLLIVLARIHEELSNENVVKYYPDLVSYDANALQVNPSKYEKWYERNVITDGLSPIDHIYRGQLENILKCQRCGNSSYSYSTFYVLSLAIP 483
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Match: EDO39905 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A7S8P2]) HSP 1 Score: 306.99 bits (785), Expect = 7.727e-85 Identity = 193/505 (38.22%), Postives = 269/505 (53.27%), Query Frame = 3
Query: 1764 ILTEDGGVLSPTLRFNE--DFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVN-------GLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRK--ASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPND----RIYLTQTLYAY 3233
ILT +GG L ++ DFEILP P+W WYG ++ R + ++E+ L L I +H P + GLS+F + SM K+ +AY A+FS+ IN + +N L L+++E+ L+DL L + +L+E RN D++WPEE++ L + KSL+ + KG TGLSN+GNTCF NSA+QC+SNT LT++F H E+N RTNP G +G +A RYG+L++D+W+GS+ + PLKL+ T+ +YA +F+G QQHDAQE LS LLD LHEDLNR+ KP Y ELKDS+GR D+ VAQEAW HI N SI+VDLF G LKS V C C S RFD ++ LSLP+PM + EI+V A P KY +L N D + + +++++ + +LLV + +K P D R L LYAY
Sbjct: 471 ILTGEGGRLKRSVPLGRVRDFEILPEPVWRALLTWYGGGPALPRTVVVPLNG------------------DCTPELELFPLNLKIYRHSPPPTRGGITTWTGVGFGLSSFG--------FNTNSMTASHAPPKKYLAYLAAFSY----------------IN--------TLQQNTLHLLEEENSSLRDLNLTDNMNLLLEVRNRDMSWPEEMSSLASSKSLREKLNLEPTEKGATGLSNLGNTCFMNSALQCLSNTQPLTQFFTAKCHYYELN----RTNPLGMKGHIARRYGDLVEDLWSGSSRSLAPLKLRWTIGRYAPRFNGFQQHDAQEFLSFLLDGLHEDLNRVHDKP-YVELKDSDGRLDEIVAQEAWDNHIKRNQSIIVDLFQGQLKSQVRCVECGYVSARFDPFTFLSLPLPMDNSIFIEIVVVPLSGATPVKYGLLLNNDDKYKA-VRKELAKLCVINVAQLLLVEVYGATVKSL--PTDSQKIRSALGGILYAY 917 HSP 2 Score: 233.417 bits (594), Expect = 6.875e-62 Identity = 136/375 (36.27%), Postives = 207/375 (55.20%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQ-KKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIE--LEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPL-LTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKP 1211
MG+ +S+LC L YEEA KR+T +E+ RL+ AF S ++ + F EV G +PS V I+ A+ T G+ F++L G+V+LT+GT EEK KF F++Y ++ + +E + A D ++ V +D L FD + W+ ++P +++WLL L LTD+S YQ +A VTHL EL+IIELEK Y L + S F+L FT L PP+P S + +F+AFDENNDG IDF E+ CGISACCRG +ER++FC ++F+ DG +R ++E + +I +++ S +K +++++ +W N + F F L+ Q+C IV GL+P
Sbjct: 1 MGLRESKLCFLPYEEAVKRVTEEEMQRLRLAFKRSSGVSGLMPQNMFSREVLGDGVPSKVCEQIYGAIG--GTGKGVTFRDLLCGLVLLTRGTREEKIKFIFNVYSSDGTYVTKDCIESLILACDGGNIPQVVSDVFSEVDR---LTFDEFSHWLLKHPDVTTLTRWLLIVSSGCSLQLTDNSETPTFYQTLATVTHLQELDIIELEKKYWFLKSASKSGKFDLETFTMLASPPIPSSACKGLFQAFDENNDGHIDFREMACGISACCRGSSSERQQFCFRVFDCDQDGFLSRDELELMSKILLQIRKENSPEK-----------------------DGKVNLEEFQVWSNLHRFSLLFPKLLVQVCHIVLGLRP 347 HSP 3 Score: 143.665 bits (361), Expect = 2.847e-34 Identity = 87/280 (31.07%), Postives = 137/280 (48.93%), Query Frame = 3
Query: 3258 EINKNGSSPSTQENLIKFVIY-HRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPA--DQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
E +G SP FV+ HRK+ + YF++ + LFG P+V+ ++ H +Y QVS ++P P K +A D ++ + +PF L V + C+ C W FC GC + + + N Y+AI+W LHL EK + + ++ +P+DL +C+ +FT +E L + L+ C KCKK + A KKL++W+LPPIL+IHLKRFQ+++ W KS+K V YP+ D + F+
Sbjct: 1009 ETPSSGPSPYLGYCFKGFVVAIHRKMNRSDAYFLSPQKNRPSLFGVPVVVPC---------------------TSRTKHCELYQTVWKQVSRLLSAPAPGDK--NAKDGNTGD--YPFELRVVQKDGVTCAWCSWFRFCRGCLIKCSDQEFG-----NSSSYIAIEWDPTTLHLRYQSSLEKNCEEHESVERSRRLQTEPIDLYECLRAFTNEEELGDEELWYCNKCKKHRLAVKKLEIWSLPPILVIHLKRFQYVNGHWVKSNKIVNYPMAGFDPSAFL 1258 HSP 4 Score: 82.0333 bits (201), Expect = 1.796e-15 Identity = 39/101 (38.61%), Postives = 60/101 (59.41%), Query Frame = 3
Query: 4179 IYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
+Y LYA++CH G L GHY++Y+ N + KWY +NDS CKE D ++ + YLLFY++ +LN FL V + V ++ E+D+K+ C +Q
Sbjct: 1432 VYRLYAMSCHSGVLGGGHYISYSHNP-NGKWYCYNDSSCKECDVNKMHVDSPYLLFYQQDDLNEKAFLPSVRDRTPDNVSD-----DEDFENDVKRMCSVQ 1526
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Match: EDO34754 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A7SNI6]) HSP 1 Score: 229.565 bits (584), Expect = 1.008e-63 Identity = 189/664 (28.46%), Postives = 303/664 (45.63%), Query Frame = 3
Query: 2433 VTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQ-LNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTEC---SEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSV---NQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRA-YKKLDLWTLPPILIIHLKRF-QFLDKRWQ------KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXX----XETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLN 4367
+TGL N GNTCF N+ IQC+++T++L EYF+ + ++ + G +G V + L+K +W + V + K + K+ Q+ G QHDAQE L LLD +HEDLNR K Y+ KD+ GR D+ +A EA H+ N S V+DLF +S + C CK S FD + LSLPIP + L +VF S P + V I + S +C +S + +S+++ + + N R Y Q L I D++ E + + E +I +Y R FG P V+ V + E++ +Q H ++ + + AD +K++ + ++ + L S HP A V + L +SDA + G + L + + + +++ + + +N + ++L C E +T E L D + C +CKK Q+ KKL LW+LP +LIIHLKRF Q L + Q K H V +PV +LD++ ++ + +S ++ T R+ D +Y+LYA+ HLG++ GHY + N +WYL++D+ ++V ++T AYLLFY R NL
Sbjct: 1 LTGLKNHGNTCFINAIIQCLAHTDLLAEYFVMGGYKQDLKLRKGQIKKFGTKGEVTEKLAVLLKSLWYSEYSGEVSSEFKSVVGKHGAQYRGYSQHDAQEFLLWLLDIVHEDLNRA-TKRKYKANKDNLGRSDECIATEALNNHLRCNDSFVLDLFQAQYRSALSCPKCKQKSTTFDPFLCLSLPIPQRSLRPLLVTIVFVGASRAPLRIGVSV-PISGSISDLCAAISEMTDIPCSSLIITELSSDGF------NRRFYANQPL--SIIHDSDAIYGFEAPQRLGIHTFTETVISPSLY---------------RCCCR-FGQPFVVHV----LQELSFSKLQALFLHEMDRMLLDN----AD-FQIKKHRQLFRLRVMNGLAGRSYLSSELDHPLYTATVERRVA---------------LLTHSDAPGSTGPAHIRMVLEWESELRNSCIKPDVQDSPEEHTSVQNVQNLFSNTHAVNLDNCFELYTKDETLGLDDAWLCPRCKKLQQGTVKKLSLWSLPEVLIIHLKRFRQLLFLQLQCPSGRTKLHTLVEFPVNDLDMSPHLEPRHKIIPDTHSVLTNWPHWRRTRRSSGVAGEDNLYDLYAVCNHLGNMSGGHYTAHCRNPTDGQWYLYDDTRVEQVPVNQVTTQAAYLLFYARRNLG 614
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Match: EDO44454 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A7RVV5]) HSP 1 Score: 219.164 bits (557), Expect = 3.880e-58 Identity = 145/455 (31.87%), Postives = 228/455 (50.11%), Query Frame = 3
Query: 2160 FSHRTRITDLIELLSKKLNINRE-EMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTL-------------------------------KSLQNRKASV--SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFE-ILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKK-FLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
FS I ++ + +I E E R+W N L+ + ++ +QD L +++EQ+N D +W + + +L + K S + G+TGL+N+GNTCF NS +QC+SNT LT+YF+ ++ E+N NP G +G +A Y +L+ IW+G ++ I P + KM + ++A QFSG QQ D+ ELL+ LLD LHEDLNRI KP Y EL+D++GR D VA EAW H N SI+VD+FHGL KS + C C SV FD LSLP+P++ ++ E L+ P ++ + + S + +C + +Q V + +++ + +N+ K F N + +I+E +E + E L+ V +K + A+Y S V LFG P++++V K
Sbjct: 165 FSKGDTIGHIVSTMKNLFSIPEESETRLWNKYMSNTFELLSNMEQSVQDAGLYQGQTLVIEQQNEDGSWQRQNTRSSYSSSTITSSSADNGYSSSNNSSSYSGYGSGRYWSNLYDNKKSYRGTPGLTGLANLGNTCFMNSGLQCLSNTMTLTKYFLSKEYVDELNT----DNPLGMKGEIAKAYADLLNQIWSGQSSSIAPRQFKMQVGRFAPQFSGYQQQDSHELLAFLLDGLHEDLNRIKMKP-YIELRDADGRPDQVVASEAWHNHRQRNDSIIVDIFHGLFKSTLVCPECNKISVTFDPLCYLSLPLPVKKERNIECFLIKNEPMEKPVQFKLTVPKMGSVAD-MCAILGKQAGVPKDKLVVTDVYNNRFHKIFKNTESLTTIMDRDDIFIYEVSERVD---------NPEYLVLPVYQRKKGARLASYTYGSSSKV--LFGIPLLISVPK 602 HSP 2 Score: 105.531 bits (262), Expect = 1.032e-22 Identity = 61/210 (29.05%), Postives = 101/210 (48.10%), Query Frame = 3
Query: 3726 EQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
+ YL++DW+ N ++ D+ + + L CI+ F T+E L + + C +CKK Q+A KK DLW LP +L+IHLKRF + K V +P+ LD++ ++ + P +Y+L ++ H G + GHY Y N E + WY ++DS +++E++I AY+LFY+R
Sbjct: 740 QSYLSLDWTTEKKEKYYNTNLSEETEEDESVARKPTQRKSVVQLNDCIQLFLTREKLGANDPWYCPQCKKHQQASKKFDLWCLPEVLVIHLKRFSYTSLWRDKIDTLVNFPLCGLDMSDYV------------------IGKDGP----PPLYDLIGVSNHYGGMGGGHYTAYCRNHEDDHWYSYDDSSVSQINEEQIVSKAAYVLFYKR 927
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Match: EDO34837 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A7SN67]) HSP 1 Score: 141.354 bits (355), Expect = 3.029e-36 Identity = 75/192 (39.06%), Postives = 112/192 (58.33%), Query Frame = 3
Query: 2424 SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWT--GSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
S G+ GL N+GNTCF NS +QC+S+T +LT ++ ++ INN S +G + + +LIK +W + + I K + ++A +F G Q DAQE L LL+ LH+DLN++ KP Y+ + N D E A++AW+ +I + S++ DLF G+LKS + C C SV FD + LSLPIP
Sbjct: 2 SDGLVGLRNLGNTCFMNSILQCLSSTWLLTNIVLKGTYVSSINNKS------SMKGRLVQAFADLIKSMWKHDATESAISAHAFKTQIQRFAPRFVGFNQQDAQEFLHFLLEGLHDDLNKVQEKPKYKMCEFDNSLSDLEKAKKAWKLYIERDNSLITDLFVGVLKSTLICLECGFKSVTFDPFWDLSLPIP 187 HSP 2 Score: 83.9593 bits (206), Expect = 6.016e-17 Identity = 58/175 (33.14%), Postives = 87/175 (49.71%), Query Frame = 3
Query: 3834 TANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRN-LDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYER 4355
T +D+ C+E FT +E+LD D C KCKK +++ KK + P IL+IHLKRF R K V +PV + +L +F P + A+Y+LYA++ H GS GHY Y + +S +W+ FNDS ++ +T AY+LF+E+
Sbjct: 200 TDGGDVDIRDCMELFTKEEVLDGDERPTCEKCKKKRKSTKKFTVQRFPRILVIHLKRFSGYGFR-SKLQTNVVFPVTSPQELGEFA---------------------ADPSEGQSAVYSLYAVSNHSGSTYGGHYTAYCKHPQSRQWHCFNDSRVDQISSSRVTGPQAYILFFEK 352
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Match: EDO36301 (Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A7SIY1]) HSP 1 Score: 114.005 bits (284), Expect = 1.149e-26 Identity = 58/166 (34.94%), Postives = 86/166 (51.81%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRW-QKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFY 4349
L CI+ FT E + D ++ C +CK+ + A K+L +W LPPILI+HLKRFQ+ D W QK V +PV LD++ +I G Y+LY ++ H G++ GHY + N + +W+ F+D + E+ +I S AYLLFY
Sbjct: 257 LRDCIKLFTRAERMSGDDMWYCPRCKQHRVASKQLQIWRLPPILIVHLKRFQY-DGMWRQKLQTAVSFPVDGLDMSGYIT-----------------------GPTKRPAYHLYGVSNHYGTMHGGHYTAFCKNAHNQRWFKFDDQVVTEMSANDIVSSAAYLLFY 398 HSP 2 Score: 100.138 bits (248), Expect = 5.376e-22 Identity = 64/187 (34.22%), Postives = 95/187 (50.80%), Query Frame = 3
Query: 2448 NIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQ 3008
N+GNTC+ NS +QC+ NT L YF+ +IN + NP G G+ Y + S N L + ++ F G Q D QE L++++D LHED+ +I KP Y + D N E A W H N S++++LF G+ KS V C +C +SV ++ +S L+LP+P N
Sbjct: 84 NLGNTCYMNSVLQCLFNTAPLAAYFVSGAFRDDIN----KENPLGTSGMYLDFY-------FITSINFSFYLT---SSPEFFTTFEGVCQQDFQEFLALMMDKLHEDVCQIRHKP-YLKAPDDN-IDPAEAANITWDNHRKRNESLMLNLFDGMFKSTVRCLVCAKDSVTYETFSNLTLPLPGNSNH 254
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Match: usp32 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:101173728]) HSP 1 Score: 486.878 bits (1252), Expect = 2.177e-145 Identity = 298/831 (35.86%), Postives = 431/831 (51.87%), Query Frame = 3
Query: 1767 LTEDGGVLSPTLRF--NEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE----------------DTECSE---------------INKNGSS---PSTQEN------------------LIKFVI-YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L L+ DFE +P P+W ++WYGA+IS+ RP+ ET+ Y + + F R Q + + + + P+ P KR +AY FS I D+ LS++L I E+MR+W EN LTL+DDED L++L + + ++++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L+ ++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SI+VDLFHG LKS V C C S RFD ++ LSLP+PM + EI V + + P +Y + N +D + + +Q+S S++P ILL + + IK F N ++ L+ + FE DT + I G S PS+ E I ++I HRK+++ YF++ + LFG P+++ + +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D +VG Y+A+DW ALHL E+ + ++ +P+ L C+++FT++E L +D LY C KCK + A KKLDLW LPPILI+HLKRFQF++ RW KS K V++P N D + F+
Sbjct: 524 LTMEGGRLKRCLQLLPGRDFETVPEPVWRALYHWYGANISLPRPVILETKTGLAELELYPRYLL-FLRQQPATRSPQSNIWVNMGNVPSPNAPL------------------------------KRMLAYTGCFSRMATIKDIHLYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLENLKIYDEQQLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFISGRHLYELN----RTNPIGMRGHMAKCYGDLVMELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVASEAWENHLRRNRSIIVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLN-MDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEVPVPGSPTSLSSPTLTDTTPTANGTAANGHVGDMAILIPNGGPSTMMPSSPETPLFNGVPNGHIMQVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTSRKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVGKDGNSCAWCPWYRFCRGCTIECSEDR-ASVGN----AYIAVDWDPTALHLRYQTSQERIVEEHCSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPILIVHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1292 HSP 2 Score: 223.787 bits (569), Expect = 1.712e-58 Identity = 136/393 (34.61%), Postives = 210/393 (53.44%), Query Frame = 3
Query: 159 ITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDI-EYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
+T EL RLK AF S ++ F EV G+ +P V+ I+ + ++ G++F L +G+V+LT+G EEK K+ FSL+ N+ E I A + + A++ + E + ++ + W+ +N +S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++ E + + E K N + D ++ D++ IL ++++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + + ++I+ W W
Sbjct: 9 VTEVELKRLKDAFKRTSGLSCYMTQQCFYREVLGNGVPPKVAEVIYTSFGGSS--KGLHFNNLTVGLVLLTRGRDEEKAKYLFSLFANDLGGYAAREDIE-AVLQVLDGEVPATLSKCFTEGDKVNYERFRSWLLQNKEAFTLSRWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPIAERQKFCFKVFDVDRDGVLSRDELHEMVVALLEVWKDNRT-DTLPELNSSVSDIVEEILKMHNTTELDHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQQGQNWFLISTFWWQTW 396 HSP 3 Score: 93.9745 bits (232), Expect = 1.033e-18 Identity = 48/103 (46.60%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
DAIYNLYAI+CH G + GHYVTYA N + KWY +NDS CKEV +EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1463 DAIYNLYAISCHSGIMGGGHYVTYAKNP-NRKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSRFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1560
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Match: usp32 (ubiquitin specific peptidase 32 [Source:NCBI gene;Acc:101173728]) HSP 1 Score: 486.878 bits (1252), Expect = 2.515e-145 Identity = 298/831 (35.86%), Postives = 431/831 (51.87%), Query Frame = 3
Query: 1767 LTEDGGVLSPTLRF--NEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFE----------------DTECSE---------------INKNGSS---PSTQEN------------------LIKFVI-YHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKP-NHALDC-DSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI 4088
LT +GG L L+ DFE +P P+W ++WYGA+IS+ RP+ ET+ Y + + F R Q + + + + P+ P KR +AY FS I D+ LS++L I E+MR+W EN LTL+DDED L++L + + ++++E RN D++WPEE++ + + K KG TGLSN+GNTCF NS+IQC+SNT LT+YFI HL E+N RTNP G +G +A YG+L+ ++W+G+ + PLKL+ T+ KYA +F+G QQ D+QELL+ LLD LHEDLNR+ KP Y ELKDS+GR D EVA EAW+ H+ N SI+VDLFHG LKS V C C S RFD ++ LSLP+PM + EI V + + P +Y + N +D + + +Q+S S++P ILL + + IK F N ++ L+ + FE DT + I G S PS+ E I ++I HRK+++ YF++ + LFG P+++ + +D+ D V QVS + SP+P + NHA DC DS+ ++PF L V + C+ C W FC GC + + D +VG Y+A+DW ALHL E+ + ++ +P+ L C+++FT++E L +D LY C KCK + A KKLDLW LPPILI+HLKRFQF++ RW KS K V++P N D + F+
Sbjct: 536 LTMEGGRLKRCLQLLPGRDFETVPEPVWRALYHWYGANISLPRPVILETKTGLAELELYPRYLL-FLRQQPATRSPQSNIWVNMGNVPSPNAPL------------------------------KRMLAYTGCFSRMATIKDIHLYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLENLKIYDEQQLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFISGRHLYELN----RTNPIGMRGHMAKCYGDLVMELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP-YVELKDSDGRPDWEVASEAWENHLRRNRSIIVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLN-MDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEVPVPGSPTSLSSPTLTDTTPTANGTAANGHVGDMAILIPNGGPSTMMPSSPETPLFNGVPNGHIMQVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPC----TVHTSRKDLYDAV---------------WIQVS-RLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVGKDGNSCAWCPWYRFCRGCTIECSED-RASVGN----AYIAVDWDPTALHLRYQTSQERIVEEHCSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPILIVHLKRFQFVNGRWIKSQKIVKFPRENFDPSAFL 1304 HSP 2 Score: 245.358 bits (625), Expect = 4.803e-65 Identity = 146/412 (35.44%), Postives = 224/412 (54.37%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKK-MLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRL-VFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDI-EYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQW 1328
MG +SR+ LS+E+A KR+T EL RLK AF S ++ F EV G+ +P V+ I+ + ++ G++F L +G+V+LT+G EEK K+ FSL+ N+ E I A + + A++ + E + ++ + W+ +N +S+WLL+ V LTDDS YQ +AGVTHL+E +II+LEK Y L + + F+L F LV PP+ S+ E +F AFDEN D IDF E+ CG+SACCRGP ER+KFC K+F+ DG +R ++ E + + E K N + D ++ D++ IL ++++++D IW + FLNL+ Q+C IV GL+P EE II WL E + + + ++I+ W W
Sbjct: 1 MGAKESRIGFLSFEDASKRVTEVELKRLKDAFKRTSGLSCYMTQQCFYREVLGNGVPPKVAEVIYTSFGGSS--KGLHFNNLTVGLVLLTRGRDEEKAKYLFSLFANDLGGYAAREDIE-AVLQVLDGEVPATLSKCFTEGDKVNYERFRSWLLQNKEAFTLSRWLLSGGVCV-TLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPIAERQKFCFKVFDVDRDGVLSRDELHEMVVALLEVWKDNRT-DTLPELNSSVSDIVEEILKMHNTTELDHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQQGQNWFLISTFWWQTW 407 HSP 3 Score: 94.3597 bits (233), Expect = 9.532e-19 Identity = 48/103 (46.60%), Postives = 63/103 (61.17%), Query Frame = 3
Query: 4173 DAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
DAIYNLYAI+CH G + GHYVTYA N + KWY +NDS CKEV +EI AY+LFYE+ ++ FL + + ++ ESD KKYC++Q
Sbjct: 1480 DAIYNLYAISCHSGIMGGGHYVTYAKNP-NRKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSRFLPKTDGKKMADTSS----MDEDFESDYKKYCVLQ 1577
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Match: usp11 (ubiquitin carboxyl-terminal hydrolase 11 [Source:NCBI gene;Acc:101166285]) HSP 1 Score: 342.813 bits (878), Expect = 1.067e-99 Identity = 231/751 (30.76%), Postives = 368/751 (49.00%), Query Frame = 3
Query: 2229 EMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFV------LAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDI 4460
E R+W +++ + + + D CL + +++E RN D TWP Q++ + GV GL+N+GNTCF NSA+QC+SNT LTEYF+++ +L E+N TNP G +G +A Y ++IK +W+G +VP K + +A QF G QQHD+QELLS LLD LHEDLNR+ K Y EL+D++GR D EVA EAW+ H N S++VD FHGL KS + C C SV FD + LS+P+P+ + E+ VF A P ++ V+ S +C +S SV P +++ + +++ K N ++ + L + GSS +++E ++ +Y R+ +Y S T LFG+P++L V + + + +++ L + ADQ + P +P + ++ + + + + C N + +N G + Y+AIDW N +K++ + + A + L +CIE FTT E L+++ + C CKK Q A KKLDLW+LP +LIIHLKRF + +K V +P+R+LD + F+ R ++ +E + Y+L A++ H G L+ GHY +YA NK++ +WY F+DS E +I + AY+LFY R + L L+N ++ + S E ++
Sbjct: 175 ECRLWMKSSDSSCERLRNVHISVLDACLSSGMTVIMEMRNSDGTWPSSRPQIMRNSVEEQDSYRGQPGVCGLTNLGNTCFMNSALQCLSNTPPLTEYFLQNSYLDELNF----TNPLGMKGEIAEAYADVIKQMWSGRHYSVVPRVFKTKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKNK-EYIELRDADGRPDQEVAGEAWRNHRRRNDSVIVDKFHGLFKSTLVCPECHKVSVTFDPFCYLSVPLPVSKERVMEVFFVFLDPHAKPVQHRVVVPKAGKVSD-LCAALSDMTSVAPTQMVVADVFNHRFYKIYNADESLSLMR------------------GSSSTSREQVL-LALYLRERSHYRDYGSGSSSYGTSLFGHPLLLNVPQ---SSCSREELYSLFLQRLASSAEEGMNACADQSAAGCTEAAP-EQPCVTTEEENTESKQEGACSPSPPPNENPAKRRACRQRRKKNLFTIQAVNSNGTTERGTGDGGTQPYVAIDWDPDMKKRFYNENEAEKYVKHSSMEVPQQQAT--VQLQECIELFTTVETLEEENPWYCPVCKKHQLATKKLDLWSLPEVLIIHLKRFSYTKFTREKLDSIVDFPLRDLDFSDFLLR------------------KSLSSEEPPSRYDLIAVSNHYGGLRDGHYTSYAKNKDNAQWYYFDDSKVTYASEDQIMTNAAYVLFYHRQDKIRKPALPSPLTNSASSTHSTNNVASSTDEGEV 876
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Match: usp4 (ubiquitin specific peptidase 4 [Source:NCBI gene;Acc:101173051]) HSP 1 Score: 313.153 bits (801), Expect = 2.850e-89 Identity = 208/669 (31.09%), Postives = 333/669 (49.78%), Query Frame = 3
Query: 2409 RKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEI-LVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPND---RIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDI-AEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNA---KSTANQP------MDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQ-KSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNA 4370
R++ G+ GLSN+GNTCF NSA+QC+SN LTEYF+ D + EIN R NP G +G +A Y +L+K +W ++ + P K + ++A QFSG QQ D+QELL+ LLD LHEDLNR+ KP Y L+D+ GR D+ VA+EAW+ H N S++VD+FHGL KS + C +C SV FD + L+LP+PM+ ++ E+ LV + P +Y + + + + +C +S+ + P ++++ + +++ K +D +I ++ Y ++ + +N +Y R+ K A S + T LFG P+++TV + ++ A++ I +++ N + +D L+ S Q+ + + S+ ++ N LP + V N +AIDW + KK D++ A + QP + L CIE FTT E L + + C CKK Q+A KK DLW+LP IL++HLKRF + ++ W+ K V +P+R+L++++F+ + P IY+L A++ H G + GHY YA NK KWY F+DS + +I AY+LFY+R + +A
Sbjct: 310 RESQAQPGLCGLSNLGNTCFMNSALQCLSNAPPLTEYFLDDQYEAEIN----RENPLGMRGEIAEAYADLVKQMWLSRSSYVAPRTFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKP-YLALRDAEGREDEIVAKEAWKNHRLRNDSVIVDIFHGLFKSTLVCPVCSKVSVTFDPFCYLTLPLPMKKDRTMEVFLVRSDPQSKPTQYRAVVPKLGTVAD-LCSALSKLCGIPPENMVVADVYNHRFHKIYRRDDGLNQIMEKDDIFVYEVQEEDSERMN--------------LPVYLRERQSK----HAASSSSTLLFGQPLLITVPRHNLTADVLYDKILERIGPEPENGLSEEEEETSD------------------LENGSKGQK---------TKLFTFSVVNFYGTANISSLPCEGN----VLKLNPHSTIAIDWDTES----------KKLFYDEQEAEAYEKHESMLQPQKKKTTVALRDCIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPRILVVHLKRFSY-NRCWRDKLDTVVDFPIRDLNMSEFVC-----------------DPKASP-----YIYDLIAVSNHYGGMGGGHYTAYAKNKLDGKWYYFDDSSVSSATDDQIVTKAAYVLFYQRRDEDA 890
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Match: USP15 (ubiquitin specific peptidase 15 [Source:NCBI gene;Acc:101156943]) HSP 1 Score: 240.35 bits (612), Expect = 1.539e-64 Identity = 147/442 (33.26%), Postives = 233/442 (52.71%), Query Frame = 3
Query: 2163 SHRTRITDLIELLSKKLN-----INREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEE------LNQLVTLKSLQ----------NRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAI--PKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRI-YLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRK 3416
+ R D I+++ K++ + +E R+W N ++ D +QD L +++EQ+N D TWP N +L S + S G+ GLSN+GNTCF NSA+QC+SNT LT+YF++D + E+N NP G +G +A Y ELIK +W+G + + P K + ++A QFSG QQ D+ ELL+ LLD LHEDLNRI KP Y +LKD+NGR D VA+EAW+ HI N SI+VD+FHGL KS + C +C SV FD + L+LP+PM+ + E+ + RL + P +Y + + S +C +S V +++ I +++ + N+ + + + Y+FE +N+ + + + ++ R+ +++ Y + T T LFG P ++ V +
Sbjct: 149 TRRFSKADTIDMIEKEMRELFSIPDEKETRLWNRYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGSTATNVKNSSYSLPSYHPYTNSYDYSDQSRPSERSGLCGLSNLGNTCFMNSAVQCLSNTPPLTDYFLKDKYTDELN----EDNPLGMKGEIARVYAELIKQLWSGKYSYVTPRPFKTQVGRFAPQFSGYQQQDSHELLAFLLDGLHEDLNRIRKKP-YIQLKDANGRPDKVVAEEAWENHIKRNDSIIVDIFHGLFKSTLVCPVCAKVSVTFDPFCYLTLPLPMKKERTLEVYLV-RLDPVAKPTQYKLTVPKVGCISD-LCTALSNLSGVPAEKMIVTDIYNHRFHRIFATNENLSSIMERDDIYVFE----VAVNRVEDT-----DHVVIPVHLREKYKQSGY----NHTSTPLFGQPFLIAVPR 570 HSP 2 Score: 116.316 bits (290), Expect = 1.105e-25 Identity = 72/218 (33.03%), Postives = 111/218 (50.92%), Query Frame = 3
Query: 3717 FNDEQYLAIDWSMAALHLSSNIKFEKKFLSD-DKNKNAKSTANQP--MDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERAN 4361
+D YL++DW F++ + D DK+++ + + L CI+ FTT+E L D + C CK+ Q+A KKLDLW+LPP+L++HLKRF + K V +P+R+LD+++F+ P + Y+L A++ H G + GHY YA NK+ KWY F+DS E +I AY+LFY+R +
Sbjct: 718 LSDRSYLSLDWEPEV----KKKYFDETIVEDFDKHESMEYKPQKKAFFKLKDCIDLFTTKEKLGADDPWYCPNCKQHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDSLVDFPLRDLDMSEFL---------------------INP-NAGPCRYDLIAVSNHYGGMGGGHYTAYAKNKDDGKWYNFDDSSVSPASEDQIVSKAAYVLFYQRQD 909
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Match: SMESG000062710.1 (SMESG000062710.1) HSP 1 Score: 2852 bits (7392), Expect = 0.000e+0 Identity = 1452/1460 (99.45%), Postives = 1455/1460 (99.66%), Query Frame = 3
Query: 102 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHXXXXXXXXXXXXXXXXXXXXXXXRPNKLTXXXXXXXXXXXXFKLASNFTILTEDGGVLSPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIKLLTLFIMKHVLPSVPTAVNGLSNFNQXXXXXXXXXXXXMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 4481
MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHSNSNGSCNSMSSFSS+TSYSDISRPNKLTQINNQNLLQINNFKLASNFTILTEDGGVLSPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEI+LLTLFIMKHVLPSVPTAVNGLSNFNQFGFSSIGLSGGSMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEIN +TNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQY AIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDNNNNVTNNSDHSSSETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ
Sbjct: 1 MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVFGSFLPSMVSNTIFKALNSNNTLSGINFKELFIGIVILTKGTYEEKCKFFFSLYCNESASGIELEYIRYAADKTTSKDYIASVRQYLDENSILPFDNYMKWIKENPAFNEISKWLLTSDFKVPLLTDDSVPLDIYQLVAGVTHLDELEIIELEKTYHRLVFNLSKDFNLCGFTDLVCPPLPESIVEKVFRAFDENNDGIIDFNELVCGISACCRGPKTERKKFCCKIFNKTMDGKFTRGDIEYIFQICEEMKYNESIDKTKSKSTIYCDLITSILSSVEMMTKNYISIDDLLIWINDYDFLDPFLNLMNQICMIVFGLKPKNIFEEELIITQWLLWEQKLKVIPYDHVYIINKSWMNQWINSFRLNNSEKEDYNKDSANKSNQTNTKISHSISMNNVFAINRKHQYDIAKSNKALYRENAKNQMNGRFVYCTKIDVSDDIVQNIYPDIISIGGDHSNSNGSCNSMSSFSSATSYSDISRPNKLTQINNQNLLQINNFKLASNFTILTEDGGVLSPTLRFNEDFEILPPPIWNCFHNWYGADISIKRPIWTETRDISKSAVAYDDHSISFRRYQTIVKVEIQLLTLFIMKHVLPSVPTAVNGLSNFNQFGFSSIGLSGGSMYKEFPSAKRQIAYRASFSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLIDDEDKLLQDLCLRNFDKILVEQRNIDLTWPEELNQLVTLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEIN----KTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYFAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDNNNNVTNNSDHSSSETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTFLQQVLSNVHNTVPQAVEIISDEVESDIKKYCLIQ 1456
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Match: SMESG000021124.1 (SMESG000021124.1) HSP 1 Score: 286.96 bits (733), Expect = 2.938e-81 Identity = 227/748 (30.35%), Postives = 351/748 (46.93%), Query Frame = 3
Query: 2160 FSHRTRITDLIELLSKKLNINREEMRIWKVIDENNLTLID-DEDKLLQDLCLRNFDKILVEQRNIDLTW---PEELNQLVTLKSLQNRKASVSK--GVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTIDSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQTLYAYIFEDTECSEINKNGSSPSTQENLIKFVIYHRKLIQKANYFMAHSRTV-TELFGYPIVLTVRKDDI--AEITNQDIQDK--VAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPFVLAQ----VNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFI---QRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFY 4349
FS + D+ + K + + +RI + +D ++ D + + D L N + I ++ ++ D W + + L S N + S + GV GL N+GNTCF NSAIQCISN LT YFI+ ++N TNP G G +A RY +L++ +W N VP K+ + K+A QFSG QHD+QEL+ LLD LHEDLNRI KP Y E DS+ + + A+EAW+ H N S++V+ FHGLLKS V C CK SV FDA+ LSLP+ + Q F I + I K +L + ++ F+ + V + + + I+ N++ + P D A I +D E + + SS + E + RTV + FG+PI+++V K++I + N+ Q + I+ N+ H+ ++ + P++K L S ++ H L + ++I++ I WS E++ D+ + H S N++ ++P+D+ C+ F E L + L+ C CK + A KK DLW LP +LI+HLKRF+ R K V +P+ +LDL+KF+ RD +E + Q GDE IY+L A++ H+G L GHY YA N + WYLF+DS ++ + AY+L Y
Sbjct: 129 FSRCKTLNDIYQYAMNKFAVTKP-LRILQKMDNGAFDVVKLDGNISIGDAGLSNGEHIFIDYQDSDHQWMNGKSKQRSVTKLISGGNSRQSDGRVQGVCGLCNLGNTCFMNSAIQCISNVPPLTYYFIKTEWKKDLNT----TNPLGMHGRLAERYHQLLEQMWVKGCNYTVPRDFKVEVAKFAPQFSGYAQHDSQELMMFLLDGLHEDLNRIQKKP-YLESSDSDDQPVQKKAEEAWKYHKMRNDSVIVENFHGLLKSTVVCPDCKYVSVTFDAFGSLSLPLVLTHPQLFNIKFYSSDKKILKAK-ILVSKSETVKDFLDKIKELFPEVAKSYLTAIEIQANRLSRVYFPQDS--------ANILKDVENLMVYELPSSDNKDE-----------------WIHLTMRTVDDQYFGWPIMVSVCKEEIDWYSLKNRIFQRLPFLNQIIGNKSEHNDCIDPKVAKIEYDKISPVVKI--YLKDKSTSKLHLIDLESPIKALQTHIRYELILDWSK-------------------EGKEKFYDPDFEIPESHPSMNMQ------------------HRPLDVENCVNEFIRTEKLGINDLWYCKMCKDQKAATKKFDLWQLPKVLILHLKRFRSGSYRRDKIDTFVEFPLTDLDLSKFVSDAHRD-------------AEDHNFQNGDE---IYDLVAVSNHMGGLGGGHYTAYANNFIDSNWYLFDDSHTSKIASDSVQTKTAYVLVY 789
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Match: SMESG000039010.1 (SMESG000039010.1) HSP 1 Score: 273.478 bits (698), Expect = 3.383e-76 Identity = 206/674 (30.56%), Postives = 324/674 (48.07%), Query Frame = 3
Query: 2424 SKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQDFEILVFERLSAIPKKYCVLFNTI--DSTSSFICEQVSRQLSVEPNSILLVSIEDNKIKKFLNPNDRIYLTQ-----TLYAY--IFEDTECSEINKNGSSPSTQENLIKFVIYHRKL----------IQKANYFMAHSRTVTELFGYPIVLTVRKDDIAEITNQDIQDKVAHILNNQIFHSHVYPADQVSVKQNSPIPIIKPNHALDCDSVNQRHPF-----VLAQVNSNIKHCSICHWSNFCNGCPLPNNSDAYNTVGTFNDEQYLAIDWSMAALHLSSNIKFEKKFLSDDKNKNAKSTANQPMDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNAD 4373
+ GV GLSN+GNTCF NSAIQCISN +T+YF+R+ ++N + NP G +G +A Y LI+++W+G + +P + K + ++A QF G QHD+QEL+ LLD LHEDLNRI KP Y E K+ + D +A++ W H+ N SI+VD FHG LKS V C C SV FD ++ LSLP+ Q + I + +++ I +S F +Q+ N I++ ++ +KI ++L N+ + TQ LY + D +I+ S + +L V H+KL +Q + +F + LF + D I I NQ + D + ++ D+ NS I +K N+ +PF V+ + NS+ +N+ PL + N + +Q++ I+WS + + K + N N + + + C++ F +E L + L+ C KCKK + A KKLD+W LP +L++HLKRF+ K + V +P++NL+L++++ + +END IY+L A++ H+G L GHY +A N ++WY F+DS VDE EI AY+L Y + N D
Sbjct: 260 TPGVCGLSNLGNTCFMNSAIQCISNVYPITDYFLRNSWTSDLN----KDNPLGMEGRIATAYANLIREMWSGKVSYALPREFKYEVAQFAPQFCGYSQHDSQELMMFLLDGLHEDLNRITNKP-YVEQKNQTNQPDHILAEQNWDYHLRRNNSIIVDYFHGQLKSKVVCPDCNFVSVTFDPFASLSLPLRHSYYQPVIFFPIDPAMPIVELSVLIYKNISLESLKDFAEKQIKWNTK---NEIIMAQVKKSKIFRYLKENELMPNTQLDDHIILYEVNALQPDHHYIDISFKEKSMYSYCSLPMIVQVHKKLNWHNFLLAIKLQMSRFFNESGFSGDNLFDF-------SDKIDFIDNQPMTDDS---------NDNISDFDKSESVSNSSIESLKTNN----------NPFENIFSVVLRENSSTS-------TNYHEINPL-----SRNRLLKSGADQHIVIEWSADGMK-----DYHKGVSRNHINCNQQIPK---LTIEDCLKQFLCEEHLGQKDLWFCPKCKKDEAASKKLDIWRLPKVLVLHLKRFKATSYFGDKINNEVLFPIKNLNLSQYVPK---------------------VKNENDYIYDLVAVSNHMGCLAGGHYFAFAKNYLDSRWYKFDDSNTSCVDESEIVTKSAYVLVYVARSCNRD 858
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Match: SMESG000020277.1 (SMESG000020277.1) HSP 1 Score: 157.147 bits (396), Expect = 2.026e-38 Identity = 84/221 (38.01%), Postives = 123/221 (55.66%), Query Frame = 3
Query: 2418 SVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQGCQGIVALRYGELIKDIWTGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKDSNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIPMQLNQ-DFE-----ILVFERLSAIPKKYC 3062
+V+ G+TGL N+GNTC+ NS +Q +SNT LT Y + + + +IN R NP G G + + + LI +WTG+ + P K +D + F G++QHD+QE L LLD LHEDLNR + + D E A ++W+ HIA N S++V +F G +S V CD C+ SV FDA+ LSLP+ N+ E L ER++ + +C
Sbjct: 623 TVTPGLTGLRNMGNTCYMNSVVQALSNTKFLTNYILTNSYQHDIN----RDNPLGYGGEIVIEFAYLITALWTGNYREVSPSDFKRMIDGKNEMFRGSEQHDSQEFLLFLLDGLHEDLNRGSKLSKVTLPVNEDKITDIERANQSWKTHIAINDSVIVRIFQGQYRSKVTCDHCQMTSVTFDAFMYLSLPLVANHNRCSLEDCLKLFLAEERMTGASQWFC 839 HSP 2 Score: 100.138 bits (248), Expect = 7.878e-21 Identity = 53/175 (30.29%), Postives = 91/175 (52.00%), Query Frame = 3
Query: 3855 LIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLNADTF 4379
L C++ F +E + + + C KCK + A KK+D+W LPP L+IHLKRF + QK + V +P+ + L ++ T + Q D Y+LYA++ H G+++ GHY ++ N ++N+W+ ++D + ++ E ++ Y+LFYE NL F
Sbjct: 818 LEDCLKLFLAEERMTGASQWFCPKCKSNRNAKKKIDIWKLPPYLLIHLKRFSCTGQYRQKLNTFVDFPMESFSLVDILKH----------------TKQPQKHD----TYHLYAVSNHHGNMESGHYTSFCKNMKANEWHNYDDDVVTKIRECDVKTPSGYILFYESYNLKQAGF 972
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Match: SMESG000053081.1 (SMESG000053081.1) HSP 1 Score: 128.257 bits (321), Expect = 5.782e-30 Identity = 80/206 (38.83%), Postives = 109/206 (52.91%), Query Frame = 3
Query: 2388 TLKSLQNRKASVSKGVTGLSNIGNTCFFNSAIQCISNTNILTEYFIRDVHLCEINNYSLRTNPQG-CQGIVALRYGELIKDIW-TGSTNLIVPLKLKMTMDKYAKQFSGNQQHDAQELLSVLLDALHEDLNRIDAKPSYRELKD--SNGRHDDEVAQEAWQAHIATNFSIVVDLFHGLLKSNVCCDICKTNSVRFDAYSMLSLPIP 2993
T+KSL N + G+ GL N+GNTCF NS +QC+SN L EY C N Y NP G + Y EL+K IW + S + + P K K K A F Q D+QE L ++L+ LH+D+N++ K + D S D E+A+ W +I + S +VDLF G LKS + C+ C S+ FD + LSLPIP
Sbjct: 265 TMKSLNN-SLNEQLGLVGLVNLGNTCFMNSVLQCLSNCKPLQEY-------CLSNKYEADLNPNSKSNGSLFKSYAELMKSIWKSDSESYVSPDKFKRIFQKLAPAFHVTSQQDSQEFLRLMLEGLHQDVNKVRRKSKSIPITDEESEKLSDKELAELYWGRYIKVDDSFIVDLFVGQLKSTLRCNKCGYRSITFDPFWDLSLPIP 462 HSP 2 Score: 106.301 bits (264), Expect = 5.382e-23 Identity = 54/173 (31.21%), Postives = 86/173 (49.71%), Query Frame = 3
Query: 3849 MDLIKCIESFTTQEMLDKDTLYQCFKCKKPQRAYKKLDLWTLPPILIIHLKRFQFLDKRWQKSHKTVRYPVRNLDLNKFIQRDXXXXXXXXXXXXXXETNRTQPGDENDAIYNLYAINCHLGSLQMGHYVTYALNKESNKWYLFNDSICKEVDEKEITMSCAYLLFYERANLN 4367
+ L C+ FT++E+LD D + +CFKCK + K+ + P +L+IHLKRF D+ K + +P RNL+L + + A YNLYA++ H G++ GHY Y N S WY ++D+ +V+E + + AY+LFY+ + N
Sbjct: 469 ISLTDCLSLFTSEEILDGDEMPKCFKCKTKRPCTKRFSIHKFPSVLVIHLKRFSG-DRYRSKISSFIEFPTRNLNLQSYAEPSTF------------------------ATYNLYAVSNHSGTVDGGHYTAYCCNPSSKNWYKYDDTRVYKVNETNVCSTEAYVLFYQMTDRN 616 The following BLAST results are available for this feature:
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Ubiquitin carboxyl-terminal hydrolase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008854 ID=SMED30008854|Name=Ubiquitin carboxyl-terminal hydrolase|organism=Schmidtea mediterranea sexual|type=transcript|length=4696bpback to top protein sequence of SMED30008854-orf-1 >SMED30008854-orf-1 ID=SMED30008854-orf-1|Name=SMED30008854-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1461bp MGVSKSRLCILSYEEAEKRITNKELNRLKRAFNEYSQKKMLSFDKFCSEVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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