SH3 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008832 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SH3 domain-containing protein vs. TrEMBL
Match: A0A267G2C0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015536g1 PE=4 SV=1) HSP 1 Score: 155.606 bits (392), Expect = 6.476e-36 Identity = 127/428 (29.67%), Postives = 245/428 (57.24%), Query Frame = 2 Query: 419 SPWLSKKMNNNVPELQNIKYK---SIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDSLKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDL---MLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKN 1684 S WLSKK++ + E Q K + TDT +L ++ R M E++ DL+ +++ K RE++T K+IENEM R+L+T D L+ K+ +R + QEK+ +++ +N +L ++L+ +DR++ +LQSEV+NLD++I K+ + E+E Q + +D+ + +L +++ ++ + + RK+ E+ K +E+ T ++ + E E + + K+ RE ++L +Q K+ + + + +LE+EN ++ +++ LE + LE ++D E + D +HDL + +KN+++++ L + +REKT++E +LQKE +LK +++LE+E YR +++ + + E ERER E + +++P++++ Q + +EKD LI +L EL+S P DL +E +N Sbjct: 114 SAWLSKKLDK-IDEKQKDSAKLPTNAQTDTKDLPNNEENRRRKMEEQLGDLKAENRAKTRELKTNKYIENEMNRKLKTADDAYVLRHTVKDKDRLINNQEKIIAELTAENAKLAEDLAERDRQVFQLQSEVDNLDRVIVAKKALKEAERLQAVGEDLDKAKDDLANANFEIETARNELKNVQSVVARKDREIKSKNDHIEQLTKQLTQAETKIAGLENEKLVADNHSKAANREAKLLKEQNDKLVDFKERCNNLEEENAYMAEAIRTLEAKILQLE---SIKHDKEVLDDLVHDLKSALKDKNQIAKDRRNLMIETQQLRDLLREKTVDEEALQKELYKLKGVLSELEKENNFYRDLDVDFRRLKQKYEEIERERFKNELVLAKLRPRVERLQNRCKEKDDLIRRLANELRSGGDRYPRDLLQEADRN 537
BLAST of SH3 domain-containing protein vs. TrEMBL
Match: A0A267FY94 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015536g2 PE=4 SV=1) HSP 1 Score: 155.606 bits (392), Expect = 6.754e-36 Identity = 127/428 (29.67%), Postives = 245/428 (57.24%), Query Frame = 2 Query: 419 SPWLSKKMNNNVPELQNIKYK---SIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDSLKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDL---MLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKN 1684 S WLSKK++ + E Q K + TDT +L ++ R M E++ DL+ +++ K RE++T K+IENEM R+L+T D L+ K+ +R + QEK+ +++ +N +L ++L+ +DR++ +LQSEV+NLD++I K+ + E+E Q + +D+ + +L +++ ++ + + RK+ E+ K +E+ T ++ + E E + + K+ RE ++L +Q K+ + + + +LE+EN ++ +++ LE + LE ++D E + D +HDL + +KN+++++ L + +REKT++E +LQKE +LK +++LE+E YR +++ + + E ERER E + +++P++++ Q + +EKD LI +L EL+S P DL +E +N Sbjct: 122 SAWLSKKLDK-IDEKQKDSAKLPTNAQTDTKDLPNNEENRRRKMEEQLGDLKAENRAKTRELKTNKYIENEMNRKLKTADDAYVLRHTVKDKDRLINNQEKIIAELTAENAKLAEDLAERDRQVFQLQSEVDNLDRVIVAKKALKEAERLQAVGEDLDKAKDDLANANFEIETARNELKNVQSVVARKDREIKSKNDHIEQLTKQLTQAETKIAGLENEKLVADNHSKAANREAKLLKEQNDKLVDFKERCNNLEEENAYMAEAIRTLEAKILQLE---SIKHDKEVLDDLVHDLKSALKDKNQIAKDRRNLMIETQQLRDLLREKTVDEEALQKELYKLKGVLSELEKENNFYRDLDVDFRRLKQKYEEIERERFKNELVLAKLRPRVERLQNRCKEKDDLIRRLANELRSGGDRYPRDLLQEADRN 545
BLAST of SH3 domain-containing protein vs. TrEMBL
Match: A0A267DMP0 (SH3 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031867g1 PE=4 SV=1) HSP 1 Score: 142.51 bits (358), Expect = 9.602e-32 Identity = 121/423 (28.61%), Postives = 234/423 (55.32%), Query Frame = 2 Query: 419 SPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDS-LKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKN 1684 SPWL+K++ +N GT R+ + +DDL+ +++ + RE++T ++IE EM R+L+ D L+ K+ ER++ QEK+ D++ DN RL ++++ K+R I +LQSEV+NLD++I K + E+E Q + ++D + EL E+++ + ++ L ++ RK E+ K ++E E+ + IA N + DS ++ EREN+ L +Q+ K+ + + ++LE+EN ++ +++ LE + LE + +E +V +L + +KN++ ++ L + + +REK+ +E LQ+E +LK +N+LE E YR E + +++ + + ER++ E + +++P++++ Q + +EKD LI +L EL+ LP +L +E +N Sbjct: 96 SPWLNKQLQQENGGAEN------GTSYASQAARRSHQPH--ESELDDLKTENRAQSRELDTNRYIEREMNRKLKIADDAYMLRHLIKDKERQISNQEKVIADLTEDNARLGEDIADKERMIFQLQSEVDNLDRVIVAKNALKEAERLQAVSEELDRTKDELASIELEVDGAKSELKSLQNQLARKEREVRAKNEQIENLNRELVEAKSS-IASAENEKAVSDSHYRTAERENKALKEQVDKLADYRERCRNLEEENAYISEAVRALEAKVFHLETLKRDKETLEELVHDLKMALKDKNKVLKDRRDLLVETQQLKDMLREKSTDEQGLQQELGKLKDVLNELERENDFYRDLETDFHKIKKRYEDLERDKFKNELLVAKLRPKVERLQKRCKEKDDLIRRLANELRGGGERLPRELLQEADQN 509
BLAST of SH3 domain-containing protein vs. TrEMBL
Match: A0A267DSA3 (SH3 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031867g3 PE=4 SV=1) HSP 1 Score: 141.739 bits (356), Expect = 1.810e-31 Identity = 121/423 (28.61%), Postives = 234/423 (55.32%), Query Frame = 2 Query: 419 SPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDS-LKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKN 1684 SPWL+K++ +N GT R+ + +DDL+ +++ + RE++T ++IE EM R+L+ D L+ K+ ER++ QEK+ D++ DN RL ++++ K+R I +LQSEV+NLD++I K + E+E Q + ++D + EL E+++ + ++ L ++ RK E+ K ++E E+ + IA N + DS ++ EREN+ L +Q+ K+ + + ++LE+EN ++ +++ LE + LE + +E +V +L + +KN++ ++ L + + +REK+ +E LQ+E +LK +N+LE E YR E + +++ + + ER++ E + +++P++++ Q + +EKD LI +L EL+ LP +L +E +N Sbjct: 96 SPWLNKQLQQENGGAEN------GTSYASQAARRSHQPH--ESELDDLKTENRAQSRELDTNRYIEREMNRKLKIADDAYMLRHLIKDKERQINNQEKVIADLTEDNARLGEDIADKERMIFQLQSEVDNLDRVIVAKNALKEAERLQAVSEELDRTKDELASIELEVDGAKSELKSLQNQLARKEREVRAKNEQIENLNRELVEAKSS-IASAENEKAVSDSHYRTAERENKALKEQVDKLADYRERCRNLEEENAYISEAVRALEAKVFHLETLKRDKETLEELVHDLKMALKDKNKVLKDRRDLLVETQQLKDMLREKSTDEEGLQQELGKLKDVLNELERENDFYRDLETDFHKIKKRYEDLERDKFKNELLVAKLRPKVERLQKRCKEKDDLIRRLANELRGGGERLPRELLQEADQN 509
BLAST of SH3 domain-containing protein vs. TrEMBL
Match: A0A267EQV6 (SH3 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031867g2 PE=4 SV=1) HSP 1 Score: 141.354 bits (355), Expect = 2.391e-31 Identity = 121/423 (28.61%), Postives = 234/423 (55.32%), Query Frame = 2 Query: 419 SPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDS-LKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKN 1684 SPWL+K++ +N GT R+ + +DDL+ +++ + RE++T ++IE EM R+L+ D L+ K+ ER++ QEK+ D++ DN RL ++++ K+R I +LQSEV+NLD++I K + E+E Q + ++D + EL E+++ + ++ L ++ RK E+ K ++E E+ + IA N + DS ++ EREN+ L +Q+ K+ + + ++LE+EN ++ +++ LE + LE + +E +V +L + +KN++ ++ L + + +REK+ +E LQ+E +LK +N+LE E YR E + +++ + + ER++ E + +++P++++ Q + +EKD LI +L EL+ LP +L +E +N Sbjct: 94 SPWLNKQLQQENGGTEN------GTSYASQAARRSHQPH--ESELDDLKTENRAQSRELDTNRYIEREMNRKLKIADDAYMLRHLIKDKERQINNQEKVIADLTEDNARLGEDIADKERMIFQLQSEVDNLDRVIVAKNALKEAERLQAVSEELDRTKDELASIELEVDGAKSELKSLQNQLARKEREVRAKNEQIENLNRELVEAKSS-IASAENEKAVSDSHYRTAERENKALKEQVDKLADYRERCRNLEEENAYISEAVRALEAKVFHLETLKRDKETLEELVHDLKMALKDKNKVLKDRRDLLVETQQLKDMLREKSTDEEGLQQELGKLKDVLNELERENDFYRDLETDFHKIKKRYEDLERDKFKNELLVAKLRPKVERSQKRCKEKDDLIRRLANELRGGGERLPRELLQEADQN 507
BLAST of SH3 domain-containing protein vs. Planmine SMEST
Match: SMESG000028773.1 (SMESG000028773.1) HSP 1 Score: 1019.61 bits (2635), Expect = 0.000e+0 Identity = 540/542 (99.63%), Postives = 540/542 (99.63%), Query Frame = 2 Query: 89 MENRGKVNKLVKRFNNKPIMKIEPPRSSNNKSALKQEIDKTEIEQDEISVTIDIGEDENPIESKPLVKTRSKKNISLKSDNEASKSNYLLNNPNPEKQVKEINKNEASQLSPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDXXXXXXXXXXXXXXXXXQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDSLKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVDELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKNDIKENMDSIS 1714 MENRGKVNKLVKRFNNKPIMKIEPPRSSNNKSALKQEIDKTEIEQDEISVTIDIGEDENPIESKPLVKTRSKKNISLKSDNEASKSN LLNNPNPEKQVKEINKNEASQLSPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDEIQKELMEKEIKLKENEQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDSLKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVV ELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKNDIKENMDSIS Sbjct: 1 MENRGKVNKLVKRFNNKPIMKIEPPRSSNNKSALKQEIDKTEIEQDEISVTIDIGEDENPIESKPLVKTRSKKNISLKSDNEASKSNDLLNNPNPEKQVKEINKNEASQLSPWLSKKMNNNVPELQNIKYKSIGTDTLELKRRQDEEIRLMNERIDDLECQSKIKDREIETLKWIENEMQRRLQTTGDHLSLKQKAKETERKLLMQEKLNQDISNDNLRLYDELSVKDRKILELQSEVNNLDKIIANKEFICESEFSQQMISKVDEIQKELMEKEIKLKENEQQISELFREIERKNVELNIKTMKLEEATDEVGRREYDYIAQERNRFNLLDSLKSKERENEILSQQLKKVDELERKWKSLEDENLFLKQSLHELEDQCNDLERRHETRNDVESVVGELHDLMLEKNRLSEENIYLSQQFSAAESKIREKTMEELSLQKENSQLKTFINDLEEEIGLYRKFEMEANEMDLQLRESERERNALENQIVEMKPQIQKYQIKSQEKDHLIMQLVRELKSQVGYLPTDLRKEISKNDIKENMDSIS 542 The following BLAST results are available for this feature:
BLAST of SH3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SH3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SH3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SH3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SH3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008832 ID=SMED30008832|Name=SH3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1800bpback to top protein sequence of SMED30008832-orf-1 >SMED30008832-orf-1 ID=SMED30008832-orf-1|Name=SMED30008832-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=543bp MENRGKVNKLVKRFNNKPIMKIEPPRSSNNKSALKQEIDKTEIEQDEISVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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