NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5

Overview
NameNADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5
Smed IDSMED30008760
Length (bp)1258
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 (SMED30008760) t-SNE clustered cells

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 (SMED30008760) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 (SMED30008760) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 (SMED30008760) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008760

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30008760SMESG000053470.1 SMESG000038413.1 SmedASXL_010271SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parenchymal cellSMED30008760SMESG000053470.1 SMESG000038413.1 dd_Smed_v4_7392_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30008760SMESG000038413.1 Contig22288newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Human
Match: NDUFAF5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:HGNC Symbol;Acc:HGNC:15899])

HSP 1 Score: 299.286 bits (765), Expect = 1.622e-98
Identity = 152/315 (48.25%), Postives = 206/315 (65.40%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            +N+FD   K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG+++++L  + +    Q D  EN L+ S E   ++    +L + E  PFKEN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC W+R   L +D M A AA+Y EMY                 R     +PAT+Q+ + I WK H SQ +PA+RGSATVSF +LGK+    P
Sbjct:   44 LNIFDRDLKRKQKNWAARQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGYIAQYLNKETIGKFFQADIAENALKNSSE--TEIPTVSVLADEEFLPFKENTFDLVVSSLSLHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQGMGESNCAWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSVPATYQIYYMIGWKYHESQARPAERGSATVSFGELGKINNLMP 339
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Human
Match: NDUFAF5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:HGNC Symbol;Acc:HGNC:15899])

HSP 1 Score: 246.514 bits (628), Expect = 2.873e-78
Identity = 137/315 (43.49%), Postives = 187/315 (59.37%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            +N+FD   K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG+++++L        +Q   C   L          + SK+ L+                 L +HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC W+R   L +D M A AA+Y EMY                 R     +PAT+Q+ + I WK H SQ +PA+RGSATVSF +LGK+    P
Sbjct:   44 LNIFDRDLKRKQKNWAARQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGYIAQYLNK------LQLFHCRKLLE---------SFSKLTLQ---------------KMLCLHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQGMGESNCAWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSVPATYQIYYMIGWKYHESQARPAERGSATVSFGELGKINNLMP 311
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Human
Match: NDUFAF5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:HGNC Symbol;Acc:HGNC:15899])

HSP 1 Score: 216.468 bits (550), Expect = 2.186e-67
Identity = 109/218 (50.00%), Postives = 152/218 (69.72%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLR 746
            +N+FD   K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG+++++L  + +    Q D  EN L+ S E   ++    +L + E  PFKEN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+
Sbjct:   44 LNIFDRDLKRKQKNWAARQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGYIAQYLNKETIGKFFQADIAENALKNSSET--EIPTVSVLADEEFLPFKENTFDLVVSSLSLHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQ 259
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Celegans
Match: K09E4.3 (pep chromosome:WBcel235:II:14123945:14126941:-1 gene:WBGene00010721.1 transcript:K09E4.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K09E4.3)

HSP 1 Score: 221.476 bits (563), Expect = 4.588e-69
Identity = 126/313 (40.26%), Postives = 193/313 (61.66%), Query Frame = 3 
Query:   99 VFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDE-ENLDVANSKILLEPETFP-FKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTE 1031
            VFD + K  QR  A   P+ +  +YL EE+ +R+AD+++D+ +   + +D GCG G ++ HL  + V  +IQ D      + S + E+ +V   +  ++ ET   F EN  DL+++S+S HWIN+LP  M++ ++ +KPD  F+G +   +TLY+LR +  LA+ ERIGG   H+SPFV ++D+GGLL+S+GF+++T+D DEI V +P++F LM+DL+ MG S+CT  R   + +DV+ A  AIY  MYS                       PATF+++ FI WKP P+  K AKRGS TVS KD+GK+  +
Sbjct:    2 VFDRQMKRGQRDWAVRQPDFKAAQYLKEEIGWRVADKVFDLTKFNPLVLDIGCGVGHIAPHLIKENVGKIIQVDMSGGMAQSSAKCEDPEVIVERRTVDEETLDGFHENQFDLLLTSMSAHWINHLPQWMRKCHDIVKPDCPFIGSMLAEDTLYELRCSLQLAELERIGGVSSHISPFVKTQDIGGLLSSAGFDMITLDSDEIQVGYPNMFALMYDLQLMGESHCTHRRNPTIRRDVLVAAEAIYQSMYSSDGKY------------------PATFKIVSFIGWKPGPNMPKAAKRGSQTVSLKDIGKIVED 296
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Celegans
Match: K09E4.3 (pep chromosome:WBcel235:II:14123945:14126606:-1 gene:WBGene00010721.1 transcript:K09E4.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K09E4.3)

HSP 1 Score: 172.94 bits (437), Expect = 2.744e-51
Identity = 100/231 (43.29%), Postives = 142/231 (61.47%), Query Frame = 3 
Query:  339 RGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTE 1031
            R  DEE LD              F EN  DL+++S+S HWIN+LP  M++ ++ +KPD  F+G +   +TLY+LR +  LA+ ERIGG   H+SPFV ++D+GGLL+S+GF+++T+D DEI V +P++F LM+DL+ MG S+CT  R   + +DV+ A  AIY  MYS                       PATF+++ FI WKP P+  K AKRGS TVS KD+GK+  +
Sbjct:   22 RTVDEETLD-------------GFHENQFDLLLTSMSAHWINHLPQWMRKCHDIVKPDCPFIGSMLAEDTLYELRCSLQLAELERIGGVSSHISPFVKTQDIGGLLSSAGFDMITLDSDEIQVGYPNMFALMYDLQLMGESHCTHRRNPTIRRDVLVAAEAIYQSMYSSDGKY------------------PATFKIVSFIGWKPGPNMPKAAKRGSQTVSLKDIGKIVED 221
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Fly
Match: CG8067 (gene:FBgn0033891 transcript:FBtr0330625)

HSP 1 Score: 280.026 bits (715), Expect = 1.958e-91
Identity = 142/332 (42.77%), Postives = 211/332 (63.55%), Query Frame = 3 
Query:   57 KLQNIVILRLSKMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP*FDSN 1052
            +L+ +  L  +  ++FD  AK  Q+ RAAL  +   Y+YL EE+ +R+ADR++DIKR F  A D GC RG+LS+H+ ++ V  L   DT    L  + +    +   K++ + E   F++NS+DLV+SSLS+HW+N+LPG   +I  +LKPDG F+  +FGG+TLY+LRS+  LA+ ER GG  PH+SPF   RD+G LLN +GF +LTID DE+ + +PS+FELM DL+GM  +N  ++R  HLS++ M A +AIY E+Y                 +   + IPATFQ+++F+ WKP P+Q +P +RG+  VS KDLG +  +    D+ 
Sbjct:   16 ELRALSSLTQTSQHIFDRNAKRLQKERAALSEDVGLYDYLKEEIGFRLADRVFDIKREFKAAADIGCSRGYLSRHILAESVEQLTLTDTSATMLEQA-QGTPGLKMVKLVKDEEQLDFEDNSLDLVISSLSLHWVNDLPGCFVRIKQSLKPDGVFIASMFGGDTLYELRSSLQLAELERKGGISPHISPFTQIRDIGSLLNRAGFTMLTIDTDELVIGYPSMFELMWDLKGMAENNAAFNRPAHLSRETMLAASAIYQELY----------------AKPNEKGIPATFQIIYFVGWKPGPNQPQPLERGTGEVSLKDLGSIIEKGGKLDTK 330
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Fly
Match: CG8067 (gene:FBgn0033891 transcript:FBtr0087595)

HSP 1 Score: 280.026 bits (715), Expect = 1.958e-91
Identity = 142/332 (42.77%), Postives = 211/332 (63.55%), Query Frame = 3 
Query:   57 KLQNIVILRLSKMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP*FDSN 1052
            +L+ +  L  +  ++FD  AK  Q+ RAAL  +   Y+YL EE+ +R+ADR++DIKR F  A D GC RG+LS+H+ ++ V  L   DT    L  + +    +   K++ + E   F++NS+DLV+SSLS+HW+N+LPG   +I  +LKPDG F+  +FGG+TLY+LRS+  LA+ ER GG  PH+SPF   RD+G LLN +GF +LTID DE+ + +PS+FELM DL+GM  +N  ++R  HLS++ M A +AIY E+Y                 +   + IPATFQ+++F+ WKP P+Q +P +RG+  VS KDLG +  +    D+ 
Sbjct:   16 ELRALSSLTQTSQHIFDRNAKRLQKERAALSEDVGLYDYLKEEIGFRLADRVFDIKREFKAAADIGCSRGYLSRHILAESVEQLTLTDTSATMLEQA-QGTPGLKMVKLVKDEEQLDFEDNSLDLVISSLSLHWVNDLPGCFVRIKQSLKPDGVFIASMFGGDTLYELRSSLQLAELERKGGISPHISPFTQIRDIGSLLNRAGFTMLTIDTDELVIGYPSMFELMWDLKGMAENNAAFNRPAHLSRETMLAASAIYQELY----------------AKPNEKGIPATFQIIYFVGWKPGPNQPQPLERGTGEVSLKDLGSIIEKGGKLDTK 330
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Zebrafish
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:794020])

HSP 1 Score: 295.434 bits (755), Expect = 1.794e-97
Identity = 150/310 (48.39%), Postives = 202/310 (65.16%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            MNVFD   K +Q+  A+   +S  Y+YL EE+  R+ADR+YD+ R F +A+D GCGR  +++HL+ + V  L   D   + LR  + +  D+    ++ + E  PFKEN+ DLV+SSLSMHWIN+LPG ++QI+  LKPDG F+G + GGETLY+LR +  LA+ ER GGF PH+SP+    DLG LL  +GFN+LT+D+DE+ V++P + E+M DL+GMG SNC W+R   L +D M A AAIY EMY                       +PATFQ+L+ I WKPH SQ KPAKRGSA VSF DL K+
Sbjct:   25 MNVFDRSMKRRQKDWASSLLDSSKYDYLREEVGSRVADRVYDVARTFPLALDVGCGRSHIAEHLSKEVVERLFLTDISSSSLR--NRKKSDIPAQCVMADEEFLPFKENTFDLVLSSLSMHWINDLPGALRQIHQVLKPDGVFIGAMVGGETLYELRCSLQLAELEREGGFAPHISPYTAVTDLGNLLGQAGFNMLTVDIDEVQVNYPGMLEVMRDLQGMGESNCAWNRKLLLQRDTMLAAAAIYKEMYGNEDGS-----------------VPATFQILYMIGWKPHDSQAKPAKRGSANVSFADLSKI 315
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Zebrafish
Match: zgc:162396 (zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190])

HSP 1 Score: 49.2914 bits (116), Expect = 3.731e-6
Identity = 34/106 (32.08%), Postives = 50/106 (47.17%), Query Frame = 3 
Query:  234 NVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILL----EPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFG 539
             +AVD GCG G  S+ LT  Y + ++  D  E+ +    EE   V     L       E  PF + S+DL+ ++ + HW +     +K+    LKP GC    LFG
Sbjct:   43 QLAVDLGCGTGQTSRPLTP-YFQQVVGIDVSESQV----EEARAVQGFPNLTYRVGTAEELPFPDASVDLLTAASAAHWFDA-ERFVKEAQRVLKPHGCL--ALFG 140
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Zebrafish
Match: zgc:162396 (zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190])

HSP 1 Score: 49.2914 bits (116), Expect = 3.731e-6
Identity = 34/106 (32.08%), Postives = 50/106 (47.17%), Query Frame = 3 
Query:  234 NVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILL----EPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFG 539
             +AVD GCG G  S+ LT  Y + ++  D  E+ +    EE   V     L       E  PF + S+DL+ ++ + HW +     +K+    LKP GC    LFG
Sbjct:   43 QLAVDLGCGTGQTSRPLTP-YFQQVVGIDVSESQV----EEARAVQGFPNLTYRVGTAEELPFPDASVDLLTAASAAHWFDA-ERFVKEAQRVLKPHGCL--ALFG 140
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Xenopus
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:549152])

HSP 1 Score: 289.656 bits (740), Expect = 3.064e-94
Identity = 148/310 (47.74%), Postives = 204/310 (65.81%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +NVFD + K +Q+  A   P + + +YL EE+  R+ADR++DI R F  A+D G GRG++S+HLT D V   +  D  E  LR  +    ++ + +++++ E  PFKEN+ DLVVSSLS+HW+N+LP   ++I+  LKPDG F+G ++GGETL++LR +  LA+ ER GGF PH+SPF    DLG L+  +GFN+LT+D DEI + +P +FE+M DL+GMG SNC W+R + L +D M A AAIY +MY                  E    +PATFQ+ + I WKPH SQ KPAKRGSATVSF DL KV
Sbjct:   88 LNVFDRQMKRRQKDWAVSQPGAHHCDYLREEVGERIADRVFDIARTFPFALDLGSGRGYISQHLTKDIVERFVHADLSEKALR--NLAASEIPSVRVIVDEEFVPFKENTFDLVVSSLSLHWVNDLPRAFQEIHRILKPDGVFIGAMYGGETLFELRCSLQLAEIEREGGFSPHISPFTAVTDLGNLMGRAGFNMLTVDADEIQIQYPGMFEVMKDLQGMGESNCAWNRRSLLHRDSMIAAAAIYQDMYG-----------------EEDGTVPATFQVYYMIGWKPHESQAKPAKRGSATVSFGDLKKV 378
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Xenopus
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:549152])

HSP 1 Score: 282.722 bits (722), Expect = 3.342e-91
Identity = 150/334 (44.91%), Postives = 206/334 (61.68%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDL---------------------RGSDEENL---DVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +NVFD + K +Q+  A   P + + +YL EE+  R+ADR++DI R F  A+D G GRG++S+HLT D V   +  D  E  L                       S + NL   ++ + +++++ E  PFKEN+ DLVVSSLS+HW+N+LP   ++I+  LKPDG F+G ++GGETL++LR +  LA+ ER GGF PH+SPF    DLG L+  +GFN+LT+D DEI + +P +FE+M DL+GMG SNC W+R + L +D M A AAIY +MY                  E    +PATFQ+ + I WKPH SQ KPAKRGSATVSF DL KV
Sbjct:   88 LNVFDRQMKRRQKDWAVSQPGAHHCDYLREEVGERIADRVFDIARTFPFALDLGSGRGYISQHLTKDIVERFVHADLSEKALVRQLPFSAASAVKALSRVLISTRSSQRNLAASEIPSVRVIVDEEFVPFKENTFDLVVSSLSLHWVNDLPRAFQEIHRILKPDGVFIGAMYGGETLFELRCSLQLAEIEREGGFSPHISPFTAVTDLGNLMGRAGFNMLTVDADEIQIQYPGMFEVMKDLQGMGESNCAWNRRSLLHRDSMIAAAAIYQDMYG-----------------EEDGTVPATFQVYYMIGWKPHESQAKPAKRGSATVSFGDLKKV 404
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Mouse
Match: Ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:MGI Symbol;Acc:MGI:1916737])

HSP 1 Score: 300.442 bits (768), Expect = 4.202e-99
Identity = 154/310 (49.68%), Postives = 208/310 (67.10%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +N+FD + K KQ++ AA  P+   ++YL EE+  R+ADR+YDI R F +A+D GCGRG++++HL  + V  + Q D  E+ L+ S E ++   N  IL + E  PF+EN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC+W+R   L +D M A AA+Y EMY                 R     IPATFQ+ H I WK H SQ +PA+RGSATVSF +L K+
Sbjct:   44 LNIFDRELKRKQKNWAARQPDPMKFDYLKEEVGSRIADRVYDIARDFPLALDIGCGRGYIAQHLDKETVGKIFQTDIAEHALKNSLETDIPTVN--ILADEEFLPFQENTFDLVVSSLSLHWVNDLPRALEQIHYVLKPDGVFVGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLKGMGESNCSWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSIPATFQIYHMIGWKYHDSQARPAERGSATVSFGELAKL 334
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Match: sp|A2APY7|NDUF5_MOUSE (Arginine-hydroxylase NDUFAF5, mitochondrial OS=Mus musculus OX=10090 GN=Ndufaf5 PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 2.939e-98
Identity = 154/310 (49.68%), Postives = 208/310 (67.10%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +N+FD + K KQ++ AA  P+   ++YL EE+  R+ADR+YDI R F +A+D GCGRG++++HL  + V  + Q D  E+ L+ S E ++   N  IL + E  PF+EN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC+W+R   L +D M A AA+Y EMY                 R     IPATFQ+ H I WK H SQ +PA+RGSATVSF +L K+
Sbjct:   44 LNIFDRELKRKQKNWAARQPDPMKFDYLKEEVGSRIADRVYDIARDFPLALDIGCGRGYIAQHLDKETVGKIFQTDIAEHALKNSLETDIPTVN--ILADEEFLPFQENTFDLVVSSLSLHWVNDLPRALEQIHYVLKPDGVFVGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLKGMGESNCSWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSIPATFQIYHMIGWKYHDSQARPAERGSATVSFGELAKL 334
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Match: sp|B2GV71|NDUF5_RAT (Arginine-hydroxylase NDUFAF5, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufaf5 PE=2 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 6.415e-98
Identity = 154/310 (49.68%), Postives = 207/310 (66.77%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +N+FD + K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG++++HL  + V  + Q D  E+ L+ S E ++   N  IL + E  PF EN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC+W+R   L +D M A AA+Y EMYS                      IPAT+Q+ H I WK H SQ +PA+RGSATVSF DL ++
Sbjct:   44 LNIFDRELKRKQKNWAARQPEPMKFDYLKEEIGSRIADRVYDIARDFPLALDIGCGRGYIAQHLNKETVGKIFQTDIAEHALKNSIETDIPTVN--ILADEEFLPFPENTFDLVVSSLSLHWVNDLPRALEQIHYVLKPDGVFVGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLKGMGESNCSWNRKALLHRDTMLAAAAVYREMYSNEDGS-----------------IPATYQIYHMIGWKYHDSQARPAERGSATVSFGDLARL 334
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Match: sp|Q5TEU4|NDUF5_HUMAN (Arginine-hydroxylase NDUFAF5, mitochondrial OS=Homo sapiens OX=9606 GN=NDUFAF5 PE=1 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 7.792e-98
Identity = 152/315 (48.25%), Postives = 206/315 (65.40%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            +N+FD   K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG+++++L  + +    Q D  EN L+ S E   ++    +L + E  PFKEN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC W+R   L +D M A AA+Y EMY                 R     +PAT+Q+ + I WK H SQ +PA+RGSATVSF +LGK+    P
Sbjct:   44 LNIFDRDLKRKQKNWAARQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGYIAQYLNKETIGKFFQADIAENALKNSSE--TEIPTVSVLADEEFLPFKENTFDLVVSSLSLHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQGMGESNCAWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSVPATYQIYYMIGWKYHESQARPAERGSATVSFGELGKINNLMP 339
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Match: sp|Q5RBS1|NDUF5_PONAB (Arginine-hydroxylase NDUFAF5, mitochondrial OS=Pongo abelii OX=9601 GN=NDUFAF5 PE=2 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 4.225e-97
Identity = 151/315 (47.94%), Postives = 207/315 (65.71%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            +N+FD   K KQ++ AA  PE   ++YL EE+  R+ADR+YDI R F +A+D GCGRG+++++L  + +    Q D  EN L+ S E   ++    +L + E  PF+EN+ DLVVSSLS+HW+N+LP  ++QI+  LKPDG F+G +FGG+TLY+LR +  LA+ ER GGF PH+SPF    DLG LL  +GFN LT+D DEI V++P +FELM DL+GMG SNC+W+R   L +D M A AA+Y EMY                 R     +PAT+Q+ + I WK H SQ +PA+RGSATVSF +LGK+    P
Sbjct:   44 LNIFDRDLKRKQKNWAAQQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGYIAQYLNKETIGKFFQADIAENALKNSLET--EIPTVSVLADEEFLPFRENTFDLVVSSLSLHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQGMGESNCSWNRKALLHRDTMLAAAAVYREMY-----------------RNEDGSVPATYQIYYMIGWKYHESQARPAERGSATVSFGELGKINNLMP 339
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Match: sp|A3KP37|NDUF5_DANRE (Arginine-hydroxylase NDUFAF5, mitochondrial OS=Danio rerio OX=7955 GN=ndufaf5 PE=2 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 1.090e-96
Identity = 150/310 (48.39%), Postives = 202/310 (65.16%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            MNVFD   K +Q+  A+   +S  Y+YL EE+  R+ADR+YD+ R F +A+D GCGR  +++HL+ + V  L   D   + LR  + +  D+    ++ + E  PFKEN+ DLV+SSLSMHWIN+LPG ++QI+  LKPDG F+G + GGETLY+LR +  LA+ ER GGF PH+SP+    DLG LL  +GFN+LT+D+DE+ V++P + E+M DL+GMG SNC W+R   L +D M A AAIY EMY                       +PATFQ+L+ I WKPH SQ KPAKRGSA VSF DL K+
Sbjct:   24 MNVFDRSMKRRQKDWASSLLDSSKYDYLREEVGSRVADRVYDVARTFPLALDVGCGRSHIAEHLSKEVVERLFLTDISSSSLR--NRKTSDIPAQCVMADEEFLPFKENTFDLVLSSLSMHWINDLPGALRQIHQVLKPDGVFIGAMVGGETLYELRCSLQLAELEREGGFAPHISPYTAVTDLGNLLGQAGFNMLTVDIDEVQVNYPGMLEVMRDLQGMGESNCAWNRKLLLQRDTMLAAAAIYKEMYGNEDGS-----------------VPATFQILYMIGWKPHDSQAKPAKRGSANVSFADLSKI 314
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Match: A0A0K8REZ2 (Putative methyltransferase OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 1.824e-104
Identity = 160/311 (51.45%), Postives = 212/311 (68.17%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVT 1025
            MNVFD KAK + ++ AA  P+ E Y+YL  E  YR+ADR++DIKR F+VAV+ GCGRG++ +HL ++ V  LIQCDT E  L        DV  ++++++ E  PF +NS+D+ +SSLS+HW+NNLPG  KQI  ALKPDG FLG +FGGETLYQLR A  LA+ ER GGFG H+SPFV  +DLGGLL+ +GF +LT+D DE+ V++P+ F LM DL+GM  +N +W R +HL +D M A A++Y ++Y                       +PATF +L FI WKPHPSQ KPA+RGS  VSFKDL  +T
Sbjct:    1 MNVFDRKAKTRHKNLAASDPDGEVYDYLKSEAGYRLADRVFDIKRKFSVAVELGCGRGYVGQHLDNEAVESLIQCDTSEGYLAHCKPPQ-DVPTTRMVVDEEFLPFADNSVDIFLSSLSLHWVNNLPGTFKQIQTALKPDGVFLGCVFGGETLYQLRGALQLAETEREGGFGAHISPFVQPQDLGGLLHQAGFTMLTLDSDELVVNYPTAFHLMADLKGMAENNASWKRKSHLHRDSMVAAASVYQQLYGKDDGT-----------------VPATFHILSFIGWKPHPSQAKPAERGSQNVSFKDLASLT 293
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Match: A0A224Z544 (NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex assembly factor 5 OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 321.627 bits (823), Expect = 4.844e-104
Identity = 168/311 (54.02%), Postives = 212/311 (68.17%), Query Frame = 3 
Query:   90 KMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            +MNVFD KAK++ ++ AAL  ++E Y+YL +E+ YR+ADR+YDIKR F  AV+ GCGRG +S HL S+ V  LI CDT +  L+       DV  +K++++ E  PF ENSID+ +SSLS+HW+NNLPG  KQ+ NALK DG FLG +FGGETLYQLR +  LA+ ER GGFG H+SPFV   DL  LLN +GF LLTID DE+TV++P+ F LM DL+GM  +N TW R +HL +D M A AAIY E+Y                       +PATF +L FI WKPHPSQ KPAKRGS TVS KDLG +
Sbjct:   56 QMNVFDRKAKMRHKTLAALDKDAEVYDYLKDEVGYRLADRVYDIKRKFPRAVELGCGRGHVSHHLDSEAVESLILCDTSQECLKRCKVPK-DVPVTKLVVDEEFLPFAENSIDIFLSSLSLHWVNNLPGTFKQVWNALKQDGAFLGCVFGGETLYQLRGSLQLAETEREGGFGAHISPFVQPTDLAALLNHAGFVLLTIDSDEMTVNYPTAFHLMADLKGMAENNVTWKRKSHLHRDTMVAAAAIYQELYGNEDGS-----------------VPATFHILSFIGWKPHPSQAKPAKRGSQTVSLKDLGSL 348
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Match: C3Y0I3 (Methyltransf_11 domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_92966 PE=4 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 1.576e-103
Identity = 160/314 (50.96%), Postives = 213/314 (67.83%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTES 1034
            MNVFD K K KQ++  A  P+ E+++YL +E+AYR+ADR+ D+ R F VA+D GCG+G++SK+L  D V  L QCDT E  L  +     +V       + E  P K+NS+DLVVS LS+HW+N+LPG ++Q+ +ALKPDGCF+GV+FGG+TL++LR +  LA+ ER GGF PHVSPF D RDLG LL  +G+ +LT+DMD++TV+FPS++ELM DL+GMG SN +W+R   L +D M A AA+Y +MY                       +PATFQLL+ I WKPH SQ KPAKRGSAT SF DL     +S
Sbjct:    1 MNVFDRKTKRKQKNWTASRPDHEDFDYLRDEVAYRIADRVCDVSRKFPVALDLGCGKGYISKYLNKDIVETLYQCDTSEKMLEHATNRPSEVPTLTFQADEEFLPCKDNSLDLVVSCLSLHWVNDLPGCLRQVWSALKPDGCFIGVMFGGDTLFELRCSLQLAETEREGGFAPHVSPFTDVRDLGNLLTRAGYTMLTMDMDDLTVNFPSMYELMADLQGMGESNASWARKAILHRDTMMAAAAVYKDMYGNEDGT-----------------VPATFQLLYMIGWKPHKSQPKPAKRGSATASFGDLSSAPPQS 297
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Match: A0A1S3HJ98 (arginine-hydroxylase NDUFAF5, mitochondrial OS=Lingula unguis OX=7574 GN=LOC106155688 PE=4 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 2.642e-103
Identity = 161/309 (52.10%), Postives = 214/309 (69.26%), Query Frame = 3 
Query:   96 NVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            N+FD KAK  QR+R AL  + E Y+Y+ +++ Y +ADRI DIKR FNV VD GCGRG +   +  D V  LIQC+  E  L     +N D+    ++ + E  PFKENS+DLVVS LS+HW+N+LPG  KQI  +LKPDG FLG +FGGETLY+LR +  LA+ ER GGFGPH+SPFV+  D+G LL+ +GFNL+T+D +EI + +PS+FE+M DL+GMG +NC W+R +H+ +D + A  AIY EMY +   +                 +PATFQ++HFI WKPHPSQ+KPAKRGSATVS KD+ K+
Sbjct:   59 NIFDRKAKKMQRNRTALMEDYEVYQYIRDKVGYSLADRIRDIKRKFNVVVDLGCGRGHIGSSMDPDTVGTLIQCEMAEMVL-AQACKNEDIKTFSVVADEEYLPFKENSLDLVVSGLSLHWVNDLPGTFKQIQRSLKPDGAFLGTVFGGETLYELRCSLQLAEIEREGGFGPHISPFVEPIDIGALLDRAGFNLVTMDTEEINIAYPSMFEVMEDLKGMGENNCAWNRKSHIQRDTLIAADAIYREMYGLEDGN-----------------LPATFQIIHFIGWKPHPSQKKPAKRGSATVSIKDIDKL 349
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Match: A0A023F7U5 (Putative methyltransferase OS=Triatoma infestans OX=30076 PE=2 SV=1)

HSP 1 Score: 319.701 bits (818), Expect = 2.999e-103
Identity = 168/334 (50.30%), Postives = 225/334 (67.37%), Query Frame = 3 
Query:   39 VKSKFKKLQNIVILRL----SKMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTT 1028
            +K+++     I +LRL    + MNVFD +AK+ Q++RA+     E Y+YL +E+ YR+ DRI+DIKR F+  +D GCGRG  SK++ SD V  LI CD  E+ L  +      V  SKI+++ E  PF+ENS+D+V+S+LS+HW+NNLPG   Q+   LK DG FLG +FGG+TL++LR++  LA+ ER GG  PH+SPF D RD+GGLLN +GF +LTID DEI V +PS+FELM DL+GM  +NCTW+R +HL K+VM A +AIY EMY                       IPATFQ+L+F+AWKP PSQ KPAKRGSAT S   LG++ T
Sbjct:   27 LKNQWPNFAYIAVLRLVHQETVMNVFDRRAKLLQKTRASNLDNVELYDYLKDEVGYRLTDRIFDIKRSFHKVIDLGCGRGSASKNVLSDCVENLIMCDMSESMLEKAVLPEESVKVSKIVVDEEDLPFEENSMDMVMSNLSLHWVNNLPGTFAQVLKCLKNDGVFLGSVFGGDTLFELRASLQLAETERAGGISPHISPFTDVRDIGGLLNQAGFTMLTIDCDEIIVKYPSMFELMWDLKGMAENNCTWARSHHLRKEVMIAASAIYKEMYGT------------------DEHIPATFQILYFVAWKPDPSQPKPAKRGSATHSLDSLGEIIT 342
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Cavefish
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:103024722])

HSP 1 Score: 294.278 bits (752), Expect = 1.364e-96
Identity = 151/310 (48.71%), Postives = 205/310 (66.13%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            MNVFD   K +Q+S A+   ++  Y+YL +E+  R+ADR+YDI R F +A+D GCG+  +++HL+ + V      D  E  LR +  +  ++    +L + E  PF+EN+ DLVVSSLSMHWIN+LPG ++QIN  LKPDG F+G + GGETLY+LR +  LA+ ER GGF PH+SP+    DLG LL+ +GFN+LT+D+DEI V++P + E+M DL+GMG SNC W+R + L +D M A AAIY EMY                       +PATF++L+ I WKPH SQ KPAKRGSATVSF DL K+
Sbjct:   58 MNVFDRNMKKRQKSWASALQDNHKYDYLRDEVGIRVADRVYDIARTFPLALDVGCGKSHIAEHLSKEVVERFFLTDISEGLLRQT--KPCEMPTHCVLADEEFLPFQENTFDLVVSSLSMHWINDLPGALRQINGVLKPDGVFIGAMVGGETLYELRCSLQLAELEREGGFSPHISPYTAVTDLGNLLSQAGFNMLTVDVDEIQVNYPGMLEVMSDLQGMGESNCAWNRRSLLHRDTMLAAAAIYKEMYGNEDGS-----------------VPATFEILYMIGWKPHESQAKPAKRGSATVSFGDLSKI 348
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Cavefish
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:103024722])

HSP 1 Score: 294.278 bits (752), Expect = 1.497e-96
Identity = 151/310 (48.71%), Postives = 205/310 (66.13%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            MNVFD   K +Q+S A+   ++  Y+YL +E+  R+ADR+YDI R F +A+D GCG+  +++HL+ + V      D  E  LR +  +  ++    +L + E  PF+EN+ DLVVSSLSMHWIN+LPG ++QIN  LKPDG F+G + GGETLY+LR +  LA+ ER GGF PH+SP+    DLG LL+ +GFN+LT+D+DEI V++P + E+M DL+GMG SNC W+R + L +D M A AAIY EMY                       +PATF++L+ I WKPH SQ KPAKRGSATVSF DL K+
Sbjct:   63 MNVFDRNMKKRQKSWASALQDNHKYDYLRDEVGIRVADRVYDIARTFPLALDVGCGKSHIAEHLSKEVVERFFLTDISEGLLRQT--KPCEMPTHCVLADEEFLPFQENTFDLVVSSLSMHWINDLPGALRQINGVLKPDGVFIGAMVGGETLYELRCSLQLAELEREGGFSPHISPYTAVTDLGNLLSQAGFNMLTVDVDEIQVNYPGMLEVMSDLQGMGESNCAWNRRSLLHRDTMLAAAAIYKEMYGNEDGS-----------------VPATFEILYMIGWKPHESQAKPAKRGSATVSFGDLSKI 353
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Cavefish
Match: zgc:162396 (putative methyltransferase DDB_G0268948 [Source:NCBI gene;Acc:103038316])

HSP 1 Score: 49.2914 bits (116), Expect = 3.134e-6
Identity = 37/114 (32.46%), Postives = 53/114 (46.49%), Query Frame = 3 
Query:  231 FNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDV---ANSKILLEP-ETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQL 560
            F + VD GCG G  +  L S + + ++  D  E+ L    EE   V    N      P E  PF + S+DL+ ++ + HW +  P  +K+    LKP GC    LFG    Y L
Sbjct:   42 FELVVDLGCGTG-QNTRLLSPHFQKVVGIDISESQL----EEARAVPGFTNLSYRAGPAEELPFPDASVDLLTAASAAHWFDAEP-FLKEATRVLKPRGCM--ALFGYLDTYDL 147
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Cavefish
Match: zgc:162396 (putative methyltransferase DDB_G0268948 [Source:NCBI gene;Acc:103038316])

HSP 1 Score: 48.521 bits (114), Expect = 5.399e-6
Identity = 28/97 (28.87%), Postives = 46/97 (47.42%), Query Frame = 3 
Query:  231 FNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCF 521
            F + VD GCG G  +  L S + + ++  D  E+ L  +         S      E  PF + S+DL+ ++ + HW +   G +K+ +  LKP GC 
Sbjct:   42 FELVVDLGCGTG-QNTRLLSPHFQKVVGIDISESQLEEARAVPGFTNLSYRTGTAEQLPFPDGSVDLLTAASAAHWFDP-QGFLKEASRVLKPHGCM 136
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Sea Lamprey
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:794020])

HSP 1 Score: 201.445 bits (511), Expect = 1.801e-63
Identity = 106/196 (54.08%), Postives = 130/196 (66.33%), Query Frame = 3 
Query:  447 SMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLR----GMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKV 1022
            S+HW+N+LP    QI+  LKPDG FLG + GGE+L++LR A  LA+ ER GGF PHVSPF  + DLGGL+  +GF +LT+DMDEI V +P++FELM DL+    GMG SNCTW+R   L +D M A AAIY EMY  +                    IPATFQ+LH I WKPH SQ KPAKRGS T SF DLG++
Sbjct:    1 SLHWVNDLPAAFHQIHRVLKPDGVFLGAMLGGESLFELRGALQLAEMEREGGFSPHVSPFTTAADLGGLMTRAGFTMLTVDMDEIVVRYPTMFELMADLQACASGMGESNCTWNRKAMLHRDTMVAAAAIYKEMYGPTEDS-----------------IPATFQVLHMIGWKPHESQAKPAKRGSQTRSFGDLGRL 179
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Sea Lamprey
Match: zgc:162396 (zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190])

HSP 1 Score: 50.8322 bits (120), Expect = 1.059e-7
Identity = 29/99 (29.29%), Postives = 49/99 (49.49%), Query Frame = 3 
Query:  237 VAVDFGCGRGFLSKHLTSDYVRYL---IQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFL 524
            +AVD GCG G  ++ L S   + L   +     E  +R    +N+    + I+   ET P  + S+ L+ + +S HW  +LP  M ++   L+P GC +
Sbjct:    1 LAVDVGCGSGQGTELLASISTKVLGIDVSPSQIEEAMRAKHLDNI----TYIVGPAETLPVDDGSVSLLTAFVSAHWF-DLPAFMHEVERVLQPRGCLV 94
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Sea Lamprey
Match: zgc:162396 (zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190])

HSP 1 Score: 48.9062 bits (115), Expect = 6.294e-7
Identity = 29/99 (29.29%), Postives = 47/99 (47.47%), Query Frame = 3 
Query:  237 VAVDFGCGRGFLSKHLTSDYVRYL---IQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFL 524
            +AVD GCG G  ++ L S     L   +     E  +     +N+    S I+   ET P  + S+ L+ + +S HW  +LP  M ++   L+P GC +
Sbjct:    1 LAVDIGCGSGQGTEILASFSTNVLGIDVSPSQIEEAMHAKHPDNI----SYIVGPAETLPVDDGSVSLLTAFVSAHWF-DLPAFMHEVERVLQPRGCLV 94
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Nematostella
Match: EDO42203 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2A7])

HSP 1 Score: 273.863 bits (699), Expect = 1.408e-89
Identity = 145/305 (47.54%), Postives = 202/305 (66.23%), Query Frame = 3 
Query:   87 SKMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVS 1001
            + ++VFD KAK +QR+RAA+  +   Y+YL +E A  ++DRI DI R F +A+D GCGRG L+KHL+ D +  L+  D+ E  L+   E  + +   K+  + E  PF++N+ DLVVSSLS+HW+N+LPG   Q+ + LKPDG F+G +F G+TL++LR A  +A+ ER GGF  HVSPF + RD+G LL  +G++L TID DEI+V +PS+FELMHDL+GMG +  + +R N L +D + A  AIY EMY +                     IPATFQ+L+FI WKP P+QQKP+ RGSAT S
Sbjct:   24 TSIDVFDRKAKRRQRNRAAMADDVHVYDYLKDE-ASCLSDRIGDIARHFPMAMDLGCGRGHLNKHLSKDQIGKLVLLDSAEKMLKQCQENEVQLL--KVHGDEEFLPFEKNTFDLVVSSLSLHWVNDLPGTFHQVLSCLKPDGAFVGAMFSGDTLFELRCALQIAEMEREGGFAAHVSPFTEMRDIGNLLTRAGYSLTTIDSDEISVGYPSMFELMHDLKGMGENGASRTRKNILHRDTLQAATAIYKEMYGLEEGG-----------------IPATFQILYFIGWKPSPTQQKPSDRGSATRS 308
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Medaka
Match: ndufaf5 (NADH:ubiquinone oxidoreductase complex assembly factor 5 [Source:NCBI gene;Acc:101161215])

HSP 1 Score: 203.371 bits (516), Expect = 7.181e-59
Identity = 107/224 (47.77%), Postives = 151/224 (67.41%), Query Frame = 3 
Query:   81 RLS-KMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSPFVDSRDXXXXXXXXXXXXXTIDMDEITVHFPSVFELMHDLRG 749
            RLS  + VF+ + K +Q+  AA   + + Y+YL +E+  R+ADR+YDI R F +A+D G G+  +++HLT + V  L+  D  +  LR        +A   +L + E  PF+ENS DLVVSSLS+HWIN+LPG +KQI+  LKPDG F+G + GGETLY+LR +  LA+ ER GGF PHVSPF    DLG LL  +GF +LT+D+D++ VH+P + E+M DL+G
Sbjct:   50 RLSGSVIVFNREMKKRQKDWAASLQDGQQYDYLRDEVGSRVADRVYDIARTFPLALDIGAGKSHIAQHLTEEVVERLLLTDVSQQALR--QRRRTPMATRCVLADEEFLPFQENSFDLVVSSLSLHWINDLPGALKQIHQVLKPDGVFIGAMVGGETLYELRCSLQLAETEREGGFSPHVSPFTAVTDLGNLLGRAGFTMLTVDVDDVQVHYPGIMEVMTDLQG 271
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Medaka
Match: zgc:162396 (putative methyltransferase DDB_G0268948 [Source:NCBI gene;Acc:101157984])

HSP 1 Score: 48.1358 bits (113), Expect = 2.053e-6
Identity = 28/101 (27.72%), Postives = 48/101 (47.52%), Query Frame = 3 
Query:  237 VAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLPGIMKQINNALKPDGCFLGVLFG 539
            +AVD GCG G  +  L + + + ++  D  E+ L            +      E  PF + S+DL+ ++ + HW  ++P  + + N  LKP GC   + FG
Sbjct:   42 LAVDLGCGTG-QNTRLLAPHFKEVVGIDISESQLGEMRSFCCRTGTA------EKLPFPDGSVDLLAAASAAHWF-DVPKFLAEANRVLKPGGCMALLGFG 134
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Match: SMESG000038413.1 (SMESG000038413.1)

HSP 1 Score: 279.256 bits (713), Expect = 4.148e-94
Identity = 134/136 (98.53%), Postives = 136/136 (100.00%), Query Frame = 3 
Query:   39 VKSKFKKLQNIVILRLSKMNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL 446
            VKSKFKKLQNIVILRLSKMNVFDTKAKVKQR+RA+LWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL
Sbjct:    6 VKSKFKKLQNIVILRLSKMNVFDTKAKVKQRNRASLWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL 141
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Match: SMESG000038413.1 (SMESG000038413.1)

HSP 1 Score: 244.973 bits (624), Expect = 6.703e-81
Identity = 116/118 (98.31%), Postives = 118/118 (100.00%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL 446
            MNVFDTKAKVKQR+RA+LWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL
Sbjct:    1 MNVFDTKAKVKQRNRASLWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL 118
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Match: SMESG000038413.1 (SMESG000038413.1)

HSP 1 Score: 247.284 bits (630), Expect = 1.525e-80
Identity = 116/124 (93.55%), Postives = 122/124 (98.39%), Query Frame = 3 
Query:   93 MNVFDTKAKVKQRSRAALWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWIN 464
            MNVFDTKAKVKQR+RA+LWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSL ++ ++
Sbjct:    1 MNVFDTKAKVKQRNRASLWPESENYEYLYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDTCENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLRVNHLS 124

HSP 2 Score: 161.77 bits (408), Expect = 1.647e-47
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 3 
Query:  777 RVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            RVNHLSKDVMFAVAAIYDEMYSISTSDSTESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP
Sbjct:  119 RVNHLSKDVMFAVAAIYDEMYSISTSDSTESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 205
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Match: SMESG000053470.1 (SMESG000053470.1)

HSP 1 Score: 224.557 bits (571), Expect = 4.658e-73
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:  690 MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 1037
            MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYSISTSD+TESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP
Sbjct:    1 MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYSISTSDNTESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP 116
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Match: SMESG000038413.1 (SMESG000038413.1)

HSP 1 Score: 176.022 bits (445), Expect = 5.793e-54
Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = 3 
Query:  690 MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYXXXXXXXXXXXNIPEKCREMSRPIPATFQLLHFIAWKPHPSQ 965
            MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYSISTSDSTESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQ
Sbjct:    1 MDEITVHFPSVFELMHDLRGMGNSNCTWSRVNHLSKDVMFAVAAIYDEMYSISTSDSTESSNIPEKCREMSRPIPATFQLLHFIAWKPHPSQ 92
The following BLAST results are available for this feature:
BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NDUFAF51.622e-9848.25NADH:ubiquinone oxidoreductase complex assembly fa... [more]
NDUFAF52.873e-7843.49NADH:ubiquinone oxidoreductase complex assembly fa... [more]
NDUFAF52.186e-6750.00NADH:ubiquinone oxidoreductase complex assembly fa... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
K09E4.34.588e-6940.26pep chromosome:WBcel235:II:14123945:14126941:-1 ge... [more]
K09E4.32.744e-5143.29pep chromosome:WBcel235:II:14123945:14126606:-1 ge... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG80671.958e-9142.77gene:FBgn0033891 transcript:FBtr0330625[more]
CG80671.958e-9142.77gene:FBgn0033891 transcript:FBtr0087595[more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ndufaf51.794e-9748.39NADH:ubiquinone oxidoreductase complex assembly fa... [more]
zgc:1623963.731e-632.08zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190][more]
zgc:1623963.731e-632.08zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190][more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ndufaf53.064e-9447.74NADH:ubiquinone oxidoreductase complex assembly fa... [more]
ndufaf53.342e-9144.91NADH:ubiquinone oxidoreductase complex assembly fa... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ndufaf54.202e-9949.68NADH:ubiquinone oxidoreductase complex assembly fa... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A2APY7|NDUF5_MOUSE2.939e-9849.68Arginine-hydroxylase NDUFAF5, mitochondrial OS=Mus... [more]
sp|B2GV71|NDUF5_RAT6.415e-9849.68Arginine-hydroxylase NDUFAF5, mitochondrial OS=Rat... [more]
sp|Q5TEU4|NDUF5_HUMAN7.792e-9848.25Arginine-hydroxylase NDUFAF5, mitochondrial OS=Hom... [more]
sp|Q5RBS1|NDUF5_PONAB4.225e-9747.94Arginine-hydroxylase NDUFAF5, mitochondrial OS=Pon... [more]
sp|A3KP37|NDUF5_DANRE1.090e-9648.39Arginine-hydroxylase NDUFAF5, mitochondrial OS=Dan... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K8REZ21.824e-10451.45Putative methyltransferase OS=Ixodes ricinus OX=34... [more]
A0A224Z5444.844e-10454.02NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex... [more]
C3Y0I31.576e-10350.96Methyltransf_11 domain-containing protein OS=Branc... [more]
A0A1S3HJ982.642e-10352.10arginine-hydroxylase NDUFAF5, mitochondrial OS=Lin... [more]
A0A023F7U52.999e-10350.30Putative methyltransferase OS=Triatoma infestans O... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ndufaf51.364e-9648.71NADH:ubiquinone oxidoreductase complex assembly fa... [more]
ndufaf51.497e-9648.71NADH:ubiquinone oxidoreductase complex assembly fa... [more]
zgc:1623963.134e-632.46putative methyltransferase DDB_G0268948 [Source:NC... [more]
zgc:1623965.399e-628.87putative methyltransferase DDB_G0268948 [Source:NC... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ndufaf51.801e-6354.08NADH:ubiquinone oxidoreductase complex assembly fa... [more]
zgc:1623961.059e-729.29zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190][more]
zgc:1623966.294e-729.29zgc:162396 [Source:ZFIN;Acc:ZDB-GENE-030131-1190][more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO422031.408e-8947.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ndufaf57.181e-5947.77NADH:ubiquinone oxidoreductase complex assembly fa... [more]
zgc:1623962.053e-627.72putative methyltransferase DDB_G0268948 [Source:NC... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038413.14.148e-9498.53SMESG000038413.1[more]
SMESG000038413.16.703e-8198.31SMESG000038413.1[more]
SMESG000038413.11.525e-8093.55SMESG000038413.1[more]
SMESG000053470.14.658e-7399.14SMESG000053470.1[more]
SMESG000038413.15.793e-54100.00SMESG000038413.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008760 ID=SMED30008760|Name=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5|organism=Schmidtea mediterranea sexual|type=transcript|length=1258bp
ATATAAATCTTATATGATAAATTATGCTAAGTATTTTGGTGAAGTCTAAA
TTTAAAAAGCTACAAAATATTGTCATTTTAAGGCTTTCTAAAATGAATGT
CTTTGATACAAAAGCTAAAGTGAAACAGAGAAGTCGAGCTGCTTTATGGC
CGGAATCAGAAAACTATGAATATTTATATGAAGAAATGGCTTATCGGATG
GCCGATAGAATATACGATATCAAGAGATGTTTCAATGTGGCTGTCGATTT
CGGTTGTGGCCGGGGATTTTTGAGCAAGCATTTGACCTCGGACTATGTGA
GATATTTGATCCAATGCGACACTTGTGAAAACGATTTGAGAGGATCTGAT
GAAGAGAATCTTGATGTTGCAAATAGCAAAATTCTATTGGAACCGGAAAC
GTTTCCATTCAAGGAAAACTCGATCGATCTGGTGGTGTCGTCATTGAGCA
TGCACTGGATCAATAATCTTCCCGGAATTATGAAGCAGATAAACAATGCT
CTAAAACCAGATGGCTGCTTCCTTGGAGTATTATTTGGAGGTGAAACTCT
TTATCAATTGAGAAGTGCATTTTACTTAGCGGACAATGAAAGGATTGGAG
GATTCGGACCTCACGTCTCGCCATTTGTAGATAGTCGTGATCTCGGAGGA
CTTTTAAATAGTTCAGGATTCAATCTATTGACTATTGATATGGATGAAAT
AACAGTTCATTTCCCATCAGTATTTGAATTAATGCATGATTTGCGCGGGA
TGGGAAATAGTAACTGTACCTGGTCTAGGGTGAATCATTTGAGTAAAGAT
GTCATGTTTGCTGTTGCTGCTATATATGATGAAATGTACTCGATATCGAC
ATCAGATAGCACCGAATCCTCAAACATCCCTGAGAAATGTCGCGAAATGA
GTCGACCGATTCCTGCGACATTCCAGTTACTCCATTTCATAGCGTGGAAG
CCTCATCCATCTCAACAAAAGCCCGCCAAACGCGGAAGTGCGACAGTCTC
GTTTAAGGATTTAGGCAAAGTCACCACAGAATCTCCATAATTTGATTCAA
ATTTCTTTAATAAATACTTGTTCAGTCTTCGATAGCAGTTGCACACGTGT
TTTCTTGATACTGTATCGTTGGAAATGGATCAGATGAATTTGTACAAATT
ATTATTTATACTGCTTTTTGCGAGTGGCTCACAGCATACCTTACGAGCAT
AATATATTTGACGAAACGTAGTGAAAGAACATCTCAATTGCTTTCATTGA
TACCAAGG
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protein sequence of SMED30008760-orf-1

>SMED30008760-orf-1 ID=SMED30008760-orf-1|Name=SMED30008760-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=339bp
MLSILVKSKFKKLQNIVILRLSKMNVFDTKAKVKQRSRAALWPESENYEY
LYEEMAYRMADRIYDIKRCFNVAVDFGCGRGFLSKHLTSDYVRYLIQCDT
CENDLRGSDEENLDVANSKILLEPETFPFKENSIDLVVSSLSMHWINNLP
GIMKQINNALKPDGCFLGVLFGGETLYQLRSAFYLADNERIGGFGPHVSP
FVDSRDLGGLLNSSGFNLLTIDMDEITVHFPSVFELMHDLRGMGNSNCTW
SRVNHLSKDVMFAVAAIYDEMYSISTSDSTESSNIPEKCREMSRPIPATF
QLLHFIAWKPHPSQQKPAKRGSATVSFKDLGKVTTESP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0002111X2 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
IPR013216Methyltransf_11
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
GO:0030961peptidyl-arginine hydroxylation
GO:0032259methylation
GO:0032981mitochondrial respiratory chain complex I assembly
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0008168methyltransferase activity
GO:0003674molecular_function
GO:0005515protein binding
GO:0016491oxidoreductase activity
GO:0016740transferase activity
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0016020membrane
GO:0031314extrinsic component of mitochondrial inner membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 26..229
e-value: 1.7E-17
score: 65.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..338
NoneNo IPR availablePANTHERPTHR13090:SF1ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIALcoord: 22..332
NoneNo IPR availablePANTHERPTHR13090UNCHARACTERIZEDcoord: 22..332
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 74..166
e-value: 1.7E-13
score: 51.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 36..310