SMED30008718

Overview
NameSMED30008718
Smed IDSMED30008718
Length (bp)448
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008718 (SMED30008718) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008718 (SMED30008718) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008718 (SMED30008718) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008718 (SMED30008718) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30008718

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30008718SMESG000008589.1 Contig2886GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30008718SMESG000058958.1 Contig2886GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
neural progenitor cellSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30008718SMESG000012332.1 SMESG000012317.1 SMESG000058958.1 SMESG000058954.1 dd_Smed_v6_246_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008718 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002455:1081..1569 +2199
v31.001419:77395..77976 +1493
Homology
[Close]
BLAST of SMED30008718 vs. Planmine SMEST
Match: SMESG000058958.1 (SMESG000058958.1)

HSP 1 Score: 256.914 bits (655), Expect = 4.392e-88
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 
Query:    1 LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK 366
            LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK
Sbjct:  115 LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK 236
[Close]
BLAST of SMED30008718 vs. Planmine SMEST
Match: SMESG000058958.1 (SMESG000058958.1)

HSP 1 Score: 256.144 bits (653), Expect = 5.484e-88
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 
Query:    1 LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK 366
            LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK
Sbjct:   96 LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIGPGSVVELASNVRGGFIYIILQK 217
[Close]
BLAST of SMED30008718 vs. Planmine SMEST
Match: SMESG000058954.1 (SMESG000058954.1)

HSP 1 Score: 184.496 bits (467), Expect = 6.582e-60
Identity = 93/147 (63.27%), Postives = 106/147 (72.11%), Query Frame = 1 
Query:    1 LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYE----------------------------RPDLTLGFYEIGPGSVVELASNVRGGFIYII 357
            LTGAQWSMKKNNEIIDDKDSLLNHIDKK FTIYFQ F+VIV C  R++SVTVTKDMTILEFRKECFKKIGEQIN+ L+LF+GESLDYE                            + +LTLGFYE+ P SV+ L   V+GGFIY++
Sbjct:   60 LTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDLFVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMM 206
The following BLAST results are available for this feature:
BLAST of SMED30008718 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008718 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000058958.14.392e-88100.00SMESG000058958.1[more]
SMESG000058958.15.484e-88100.00SMESG000058958.1[more]
SMESG000058954.16.582e-6063.27SMESG000058954.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008718 ID=SMED30008718|Name=SMED30008718|organism=Schmidtea mediterranea sexual|type=transcript|length=448bp
CTTACCGGAGCGCAATGGTCAATGAAAAAGAACAACGAGATCATTGATGA
TAAAGACAGTTTATTGAATCACATTGATAAGAAAATGTTTACAATATATT
TCCAGCCATTTATTGTCATTGTTAAATGCGCCGGTCGAAGCCATTCTGTG
ACCGTGACCAAGGACATGACTATTCTCGAATTCAGAAAAGAGTGCTTTAA
GAAAATTGGCGAACAAATAAATGATCTTTTAGTTTTATTTGATGGTGAAT
CTCTAGATTATGAAAGACCTGACTTAACATTGGGATTTTATGAGATTGGA
CCTGGTTCGGTAGTGGAGTTAGCTTCCAATGTAAGAGGCGGTTTCATTTA
TATTATTTTACAAAAATAAAAATAAATGTACCGGATTTTTTTCTGTATTT
CATACCATTGAATTTTATATTTTATTTAATAAAATGTGGTTCTTAAAA
back to top

protein sequence of SMED30008718-orf-1

>SMED30008718-orf-1 ID=SMED30008718-orf-1|Name=SMED30008718-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=123bp
LTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSV
TVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYERPDLTLGFYEIG
PGSVVELASNVRGGFIYIILQK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000012neural progenitor cell
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000110pharynx progenitor cell
PLANA:0000429neoblast
PLANA:0000463cholinergic neuron
PLANA:0000484secretory system
Vocabulary: INTERPRO
TermDefinition
IPR000626Ubiquitin-like_dom
IPR029071Ubiquitin-like_domsf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000626Ubiquitin-like domainSMARTSM00213ubq_7coord: 37..110
e-value: 0.0018
score: 27.5
IPR000626Ubiquitin-like domainPFAMPF00240ubiquitincoord: 40..109
e-value: 5.9E-6
score: 25.9
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 37..114
score: 10.832
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 6..115
e-value: 2.1E-7
score: 32.7
NoneNo IPR availableCDDcd17039Ubl_ubiquitin_likecoord: 39..107
e-value: 6.18674E-7
score: 41.813
IPR029071Ubiquitin-like domain superfamilySUPERFAMILYSSF54236Ubiquitin-likecoord: 26..115