SMED30008714

Overview
NameSMED30008714
Smed IDSMED30008714
Length (bp)1283
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008714 (SMED30008714) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008714 (SMED30008714) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008714

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30008714SMESG000071921.1 Contig5607_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30008714SMESG000071921.1 Contig5607_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30008714SMESG000071921.1 Contig5607_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008714 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005768:8798..10072 -6375
Homology
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BLAST of SMED30008714 vs. Planmine SMEST
Match: SMESG000071921.1 (SMESG000071921.1)

HSP 1 Score: 688.337 bits (1775), Expect = 0.000e+0
Identity = 361/378 (95.50%), Postives = 362/378 (95.77%), Query Frame = 1 
Query:   85 MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANXXXXXXXXXXXXYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVLSMFLYIFNRNITLSSAVNHLADNSTAIQAYFSILLKLLTPCCIVATTIFIATYRAIMAYKIKSRYSLKSKYRWLMASSPKLIRSTSENSSIDYLNRDSLAEEKTHKKFVSIFRFSRFMIFLLIPVYFFSLWISASWLLNETHTIISLEHTFSTVYYKITSSYPLNLRELFLVLSQMIMFMINFISVLLCFNLIIFNNTIRRVK 1218
            MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANSISFSLMMISFIYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVL                +NHLADNSTAIQAYFSILLKLLTPCCIVATTIFIATYRAIMAYKIKSRYSLKSKYRWLMASSPKLIRSTSENSSIDYLNRDSLAEEKTHKKFVSIFRFSRFMIFLLIPVYFFSLWISASWLLNETHTIISLEHTFSTVYYKITSSYPLNLRELFLVLSQMIMFMINFISVLLCFNLIIFNNTIRRVK
Sbjct:    1 MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANSISFSLMMISFIYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVL----------------INHLADNSTAIQAYFSILLKLLTPCCIVATTIFIATYRAIMAYKIKSRYSLKSKYRWLMASSPKLIRSTSENSSIDYLNRDSLAEEKTHKKFVSIFRFSRFMIFLLIPVYFFSLWISASWLLNETHTIISLEHTFSTVYYKITSSYPLNLRELFLVLSQMIMFMINFISVLLCFNLIIFNNTIRRVK 362
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BLAST of SMED30008714 vs. Planmine SMEST
Match: SMESG000071921.1 (SMESG000071921.1)

HSP 1 Score: 329.717 bits (844), Expect = 6.412e-113
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 1 
Query:   85 MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANXXXXXXXXXXXXYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVLS 612
            MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANSISFSLMMISFIYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVL+
Sbjct:    1 MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNPLFILLLANSISFSLMMISFIYTLRKYWKFLLSKAMPIIPIIFGAVTFSGLITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLATLRLKLMNIASLWTILIITGFKIVLT 176

HSP 2 Score: 47.3654 bits (111), Expect = 4.660e-6
Identity = 22/23 (95.65%), Postives = 22/23 (95.65%), Query Frame = 2 
Query:  653 AQSITWRIIRQLFKRISQFYSNY 721
             QSITWRIIRQLFKRISQFYSNY
Sbjct:  176 TQSITWRIIRQLFKRISQFYSNY 198
The following BLAST results are available for this feature:
BLAST of SMED30008714 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008714 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000071921.10.000e+095.50SMESG000071921.1[more]
SMESG000071921.16.412e-11399.43SMESG000071921.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008714 ID=SMED30008714|Name=SMED30008714|organism=Schmidtea mediterranea sexual|type=transcript|length=1283bp
GTTTGTTTTGCTGCAATGAAATGGACAACCAATGAATATATTGATAATTA
ATAAGAAACCGAGCGTTAACCGATAAATATTTCAATGGAATTAGAAAGTG
ATTATCAAATCGAGCCCCCCACAGGAACATGCCCATTCAATCCGATTTGC
TCAAATTGCATTTGTTGGTTGCATAAGAGTTCCCAGCTGTTCAATCCGGC
CAGTAAAGAAAACTTTGCGAACATTTCGAATCCACTTTTCATTTTATTGC
TGGCCAATTCCATTTCATTCAGTCTGATGATGATTTCATTTATTTACACG
CTAAGAAAATATTGGAAATTTCTCCTATCAAAAGCAATGCCAATTATTCC
AATAATTTTCGGAGCTGTGACTTTTAGCGGCCTGATCACATTTCAATCAT
TTGTCGATTGGCTCAGTATCCCATCGGTGCAATTCATGGAGACAATTAAA
TATCAAAACTTTCTTCATTTCTACATAATCTCAACGTTTTTAATTCTAAA
TTCCCTTGCTGTCATGTTGGGAATTCTTTTGGCAACATTACGACTGAAAT
TGATGAATATTGCGTCGCTTTGGACTATTCTTATTATAACCGGATTCAAG
ATTGTCTTAAGTATGTTCCTATATATTTTCAATAGAAATATAACGCTTTC
AAGCGCAGTCAATCACTTGGCGGATAATTCGACAGCTATTCAAGCGTATT
TCTCAATTCTACTCAAATTACTGACTCCCTGTTGCATAGTTGCGACAACG
ATTTTCATTGCAACCTATCGAGCGATAATGGCATACAAAATCAAATCTCG
CTACAGCTTAAAGTCAAAATACCGCTGGCTAATGGCGTCAAGTCCTAAAC
TAATAAGATCGACATCAGAGAACAGCAGCATTGACTACCTTAACAGAGAC
AGCTTAGCCGAAGAGAAAACACATAAGAAATTTGTTTCCATTTTTCGATT
TTCTCGTTTTATGATATTTCTATTGATACCGGTTTACTTTTTTAGTCTGT
GGATTTCTGCATCTTGGCTGTTAAACGAGACCCATACGATCATATCTCTA
GAGCATACATTTTCCACAGTTTACTACAAAATAACCTCATCTTATCCATT
AAATTTAAGAGAACTATTTTTAGTTTTATCCCAGATGATTATGTTCATGA
TTAATTTTATTAGTGTATTATTATGTTTTAATTTGATAATTTTCAACAAT
ACCATCCGTCGGGTGAAATGACAGGAAATTCATTTTAATTTTTATCTTTT
TCTGAAATAAATTTTTGCTCTGGGAAAAAAAAA
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protein sequence of SMED30008714-orf-1

>SMED30008714-orf-1 ID=SMED30008714-orf-1|Name=SMED30008714-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=379bp
MELESDYQIEPPTGTCPFNPICSNCICWLHKSSQLFNPASKENFANISNP
LFILLLANSISFSLMMISFIYTLRKYWKFLLSKAMPIIPIIFGAVTFSGL
ITFQSFVDWLSIPSVQFMETIKYQNFLHFYIISTFLILNSLAVMLGILLA
TLRLKLMNIASLWTILIITGFKIVLSMFLYIFNRNITLSSAVNHLADNST
AIQAYFSILLKLLTPCCIVATTIFIATYRAIMAYKIKSRYSLKSKYRWLM
ASSPKLIRSTSENSSIDYLNRDSLAEEKTHKKFVSIFRFSRFMIFLLIPV
YFFSLWISASWLLNETHTIISLEHTFSTVYYKITSSYPLNLRELFLVLSQ
MIMFMINFISVLLCFNLIIFNNTIRRVK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 85..107
NoneNo IPR availableTMHMMTMhelixcoord: 51..73
NoneNo IPR availableTMHMMTMhelixcoord: 204..226
NoneNo IPR availableTMHMMTMhelixcoord: 347..369
NoneNo IPR availableTMHMMTMhelixcoord: 162..184
NoneNo IPR availableTMHMMTMhelixcoord: 287..309
NoneNo IPR availableTMHMMTMhelixcoord: 127..149