hypothetical protein

Overview
Namehypothetical protein (AFJ24818.1)
Smed IDSMED30008690
Length (bp)3495
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of hypothetical protein (SMED30008690) t-SNE clustered cells

Violin plots show distribution of expression levels for hypothetical protein (SMED30008690) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of hypothetical protein (SMED30008690) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for hypothetical protein (SMED30008690) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008690

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008690 SmedASXL_069591SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30008690 SmedASXL_069591SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
head regionSMED30008690SMESG000019707.1 dd_Smed_v6_9352_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of hypothetical protein vs. Ensembl Human
Match: ZBED8 (zinc finger BED-type containing 8 [Source:HGNC Symbol;Acc:HGNC:30804])

HSP 1 Score: 58.9214 bits (141), Expect = 1.885e-14
Identity = 39/121 (32.23%), Postives = 57/121 (47.11%), Query Frame = 1 
Query:  625 LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQIN---SGMVEQMLQIMMFNQ*NLKRVHF--DSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
             TCT ++   Q+P   + ++V SN  L+P KL    N    G+    L        +LK +    D   T +   + S E +L  A Y   Y  AK K PH + E ++KPCAL++  I LG
Sbjct:   16 FTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLN-------SLKHMPAPSDQSETLKAFGVASHEDTLLQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLG 129

HSP 2 Score: 41.5874 bits (96), Expect = 1.885e-14
Identity = 19/49 (38.78%), Postives = 34/49 (69.39%), Query Frame = 2 
Query: 1034 DLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDIYDGAI 1180
            ++S +I  Q + DI+AS LK+ +QL +T D+ +CSQL++ ++ I +  I
Sbjct:  152 EMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRYIKEREI 200
[Close]
BLAST of hypothetical protein vs. Ensembl Human
Match: ZBED8 (zinc finger BED-type containing 8 [Source:HGNC Symbol;Acc:HGNC:30804])

HSP 1 Score: 58.9214 bits (141), Expect = 1.885e-14
Identity = 39/121 (32.23%), Postives = 57/121 (47.11%), Query Frame = 1 
Query:  625 LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQIN---SGMVEQMLQIMMFNQ*NLKRVHF--DSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
             TCT ++   Q+P   + ++V SN  L+P KL    N    G+    L        +LK +    D   T +   + S E +L  A Y   Y  AK K PH + E ++KPCAL++  I LG
Sbjct:   16 FTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLN-------SLKHMPAPSDQSETLKAFGVASHEDTLLQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLG 129

HSP 2 Score: 41.5874 bits (96), Expect = 1.885e-14
Identity = 19/49 (38.78%), Postives = 34/49 (69.39%), Query Frame = 2 
Query: 1034 DLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDIYDGAI 1180
            ++S +I  Q + DI+AS LK+ +QL +T D+ +CSQL++ ++ I +  I
Sbjct:  152 EMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRYIKEREI 200
[Close]
BLAST of hypothetical protein vs. UniProt/SwissProt
Match: sp|A4Z945|ZBED8_BOVIN (Protein ZBED8 OS=Bos taurus OX=9913 GN=ZBED8 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.507e-14
Identity = 41/118 (34.75%), Postives = 56/118 (47.46%), Query Frame = 1 
Query:  625 LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQINSGMVEQMLQIMMFNQ*NLKRVHF--DSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
             TC  +I   Q+P   + ++V SN  L+P KL    N     Q   I   +  +LK V    D   T +   + S E +L  A Y   Y  AK K PH I E ++KPCAL++  I LG
Sbjct:   16 FTCVTEIDGTQRPQCVLCNSVFSNADLRPSKLSDHFN----RQHGGIGGHDLSSLKHVPVPADQSETLKTFGVASQEDALLQASYQFAYLCAKEKNPHTIAEKLVKPCALEIAQIVLG 129

HSP 2 Score: 41.9726 bits (97), Expect = 1.507e-14
Identity = 19/49 (38.78%), Postives = 34/49 (69.39%), Query Frame = 2 
Query: 1034 DLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDIYDGAI 1180
            ++S +I  Q + DI+AS LK+ +QL +T D+ +CSQL++ ++ I +  I
Sbjct:  152 EMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRYIREREI 200
[Close]
BLAST of hypothetical protein vs. UniProt/SwissProt
Match: sp|Q8IZ13|ZBED8_HUMAN (Protein ZBED8 OS=Homo sapiens OX=9606 GN=ZBED8 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 8.386e-14
Identity = 39/121 (32.23%), Postives = 57/121 (47.11%), Query Frame = 1 
Query:  625 LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQIN---SGMVEQMLQIMMFNQ*NLKRVHF--DSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
             TCT ++   Q+P   + ++V SN  L+P KL    N    G+    L        +LK +    D   T +   + S E +L  A Y   Y  AK K PH + E ++KPCAL++  I LG
Sbjct:   16 FTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLN-------SLKHMPAPSDQSETLKAFGVASHEDTLLQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLG 129

HSP 2 Score: 41.5874 bits (96), Expect = 8.386e-14
Identity = 19/49 (38.78%), Postives = 34/49 (69.39%), Query Frame = 2 
Query: 1034 DLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDIYDGAI 1180
            ++S +I  Q + DI+AS LK+ +QL +T D+ +CSQL++ ++ I +  I
Sbjct:  152 EMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRYIKEREI 200
[Close]
BLAST of hypothetical protein vs. TrEMBL
Match: I1ZIG4 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 447.203 bits (1149), Expect = 1.050e-145
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 1 
Query: 2692 YITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII 3360
            YITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII
Sbjct:   14 YITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII 236
[Close]
BLAST of hypothetical protein vs. TrEMBL
Match: T1FCQ4 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20206603 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.554e-18
Identity = 50/138 (36.23%), Postives = 81/138 (58.70%), Query Frame = 1 
Query: 2008 VNSCK*NGIVTKIKPEMVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITKDSVELQKLYRGEAQ-----IKESKNTGWRDNLKFLFGLCDAFKLYRKCENLPLIKLRKLQSLHAASWNSRAL 2406
            VN+   +G+V +++   V+ G   P +IGCQHH LDR+L+HV D  + +    P +NY F+ +I KD   L+K +  +A+     +KE +  GWRD++KFL  L   FK ++  + +P +  + L  +  A WNSRA+
Sbjct:  239 VNTGSKSGVVVRLQKLFVQHGESAPVFIGCQHHILDRVLRHVCDALLGDSTESPNMNYPFVSKILKDYEMLKKDFESKAKKCTPNLKE-QEVGWRDDMKFLHHLICVFKHFKNFQKIPKVNFKALPGISNARWNSRAI 375
[Close]
BLAST of hypothetical protein vs. TrEMBL
Match: T1EPU1 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20198591 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.691e-18
Identity = 50/138 (36.23%), Postives = 81/138 (58.70%), Query Frame = 1 
Query: 2008 VNSCK*NGIVTKIKPEMVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITKDSVELQKLYRGEAQ-----IKESKNTGWRDNLKFLFGLCDAFKLYRKCENLPLIKLRKLQSLHAASWNSRAL 2406
            VN+   +G+V +++   V+ G   P +IGCQHH LDR+L+HV D  + +    P +NY F+ +I KD   L+K +  +A+     +KE +  GWRD++KFL  L   FK ++  + +P +  + L  +  A WNSRA+
Sbjct:  284 VNTGSKSGVVVRLQKLFVQHGESAPVFIGCQHHILDRVLRHVCDALLGDSTESPNMNYPFVSKILKDYEMLKKDFESKAKKCTPNLKE-QEVGWRDDMKFLHHLICVFKHFKNFQQMPKVNFKALPGISNARWNSRAI 420
[Close]
BLAST of hypothetical protein vs. TrEMBL
Match: T1FGE5 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20207894 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.994e-18
Identity = 50/138 (36.23%), Postives = 81/138 (58.70%), Query Frame = 1 
Query: 2008 VNSCK*NGIVTKIKPEMVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITKDSVELQKLYRGEAQ-----IKESKNTGWRDNLKFLFGLCDAFKLYRKCENLPLIKLRKLQSLHAASWNSRAL 2406
            VN+   +G+V +++   V+ G   P +IGCQHH LDR+L+HV D  + +    P +NY F+ +I KD   L+K +  +A+     +KE +  GWRD++KFL  L   FK ++  + +P +  + L  +  A WNSRA+
Sbjct:  357 VNTESKSGVVVRLQKLFVQHGESAPVFIGCQHHILDRVLRHVCDALLGDSTESPNMNYPFVSKILKDYEMLKKDFESKAKKCTLNLKE-QEVGWRDDMKFLHHLICVFKHFKNFQQMPKVNFKALPGISNAQWNSRAI 493
[Close]
BLAST of hypothetical protein vs. TrEMBL
Match: T1F4F5 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20203704 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 4.288e-17
Identity = 51/144 (35.42%), Postives = 80/144 (55.56%), Query Frame = 1 
Query: 2008 VNSCK*NGIVTKIKPEMVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITKDSVELQKLYRGEAQ-----IKESKNTGWRDNLKFLFGLCDAFKLYRKCENLPLIKLRKLQSLHAASWNSRAL*HT*WL 2424
            VN+   +G+V +++   V+ G   P +IGCQHH LDR+L+HV D  + N    P +NY F+ +I KD   L+K +  +A+     +KE +  GWRD++KFL  L   FK ++  + +P +  + L  +  A WN     H  WL
Sbjct:  207 VNTGSKSGVVVRLQKLFVQHGESAPVFIGCQHHILDRVLRHVCDALLGNSTESPNINYPFVSKILKDYEMLKKDFESKAKKCTPNLKE-QEVGWRDDMKFLHHLICVFKHFKNFQQIPKVNFKALPGISNARWNLEQFLHC-WL 348
[Close]
BLAST of hypothetical protein vs. Ensembl Medaka
Match: ENSORLT00000032191.1 (pep primary_assembly:ASM223467v1:7:34400406:34401509:1 gene:ENSORLG00000024356.1 transcript:ENSORLT00000032191.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 2.255e-16
Identity = 39/124 (31.45%), Postives = 62/124 (50.00%), Query Frame = 1 
Query:  601 YLYFVFT*LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQINSGMVEQMLQIMMFNQ*NLKRVHFDSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
            Y+ + F+   C  ++   Q+P   I +A +SN++L P KL + I     +   +     +  +KR  FD +AT      +   K +  A Y + Y +AK  EPH I E ++KP ALKM  + LG
Sbjct:   12 YVQYGFS---CITELDGSQRPICMICNAKLSNSNLAPAKLKEHIEKLHGDGEYKNTTLAEFKVKRARFDDKATLPALGFVPINKPILTASYEVAYLIAKQGEPHTIGETLVKPAALKMANLILG 132

HSP 2 Score: 40.817 bits (94), Expect = 2.255e-16
Identity = 21/51 (41.18%), Postives = 33/51 (64.71%), Query Frame = 2 
Query: 1028 MGDLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDIYDGAI 1180
            +GD+S++I  Q +AD+ +S  K  LQL++T DV N SQL   ++ + D  I
Sbjct:  154 IGDMSNDILAQVVADLISSPAKFSLQLDETTDVANLSQLAVFVRYVKDDMI 204
[Close]
BLAST of hypothetical protein vs. Planmine SMEST
Match: SMESG000019707.1 (SMESG000019707.1)

HSP 1 Score: 461.84 bits (1187), Expect = 1.886e-151
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 1 
Query: 2596 VDSSDQSFWIRNXXXXXXXXXXXXXXXXXXXXYITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII 3360
            VDSSDQSFWIRNLFYIILSIIGLILIFSAIKIYITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII
Sbjct:  203 VDSSDQSFWIRNLFYIILSIIGLILIFSAIKIYITNFVISKHRRTTKKSSTRDSPLYSLSLGDSDVESAYDDIEKCSETESKKNSSLIISEDEETQEEILGTSKEYLDSVYNNNVFHPQLVSPRKQCVEKKSHTVPVPVELHSLSSYKLQDCPTNIMTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKIITSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII 457

HSP 2 Score: 122.094 bits (305), Expect = 6.584e-29
Identity = 55/55 (100.00%), Postives = 55/55 (100.00%), Query Frame = 2 
Query: 1412 EKFCNETNGFWCGDLCIDVKLRCDGREDCKIFSNSNLESADEMNCTKYNRSFGYI 1576
            EKFCNETNGFWCGDLCIDVKLRCDGREDCKIFSNSNLESADEMNCTKYNRSFGYI
Sbjct:  147 EKFCNETNGFWCGDLCIDVKLRCDGREDCKIFSNSNLESADEMNCTKYNRSFGYI 201
[Close]
BLAST of hypothetical protein vs. Planmine SMEST
Match: SMESG000067084.1 (SMESG000067084.1)

HSP 1 Score: 64.6994 bits (156), Expect = 9.086e-12
Identity = 39/117 (33.33%), Postives = 59/117 (50.43%), Query Frame = 1 
Query: 2008 VNSCK*NGIVTKIKPEMVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITKDSVELQKLYRG--EAQIKESKNTGWRDNLKFL----FGLCDAFKLYRKCEN 2340
             NS   NGIVT+++    +     PQYI CQH+ LDR+L+H  ++ IS     P ++Y F+ +   +   L + +    ++ I    N GWRD +  L    F L    K+YRK  N
Sbjct:   31 TNSASKNGIVTQLQKYRKQAHVYSPQYISCQHYVLDRVLRHAMEFDISGKTFNPNISYPFVSKTNANYESLNRKFGSHEDSPILTESNIGWRDKINILKFSDFHLNK--KMYRKFVN 145
[Close]
BLAST of hypothetical protein vs. Planmine SMEST
Match: SMESG000028317.1 (SMESG000028317.1)

HSP 1 Score: 45.0542 bits (105), Expect = 3.530e-7
Identity = 36/124 (29.03%), Postives = 57/124 (45.97%), Query Frame = 1 
Query:  601 YLYFVFT*LTCTGKIGDLQKPHWKIIDAVISNTHLKPFKLMKQINSGMVEQMLQIMMFNQ*NLKRVHFDSRATHQNSALISSEKSL*MA*YHIDYKVAKSKEPHVITENMIKPCALKMNAIFLG 972
            YL + F+     G+    Q+P   I   V++N  +KP KL K + +       + + F +   K ++     +H     I  +  L  A + I Y +AK K P  I E ++KP A+KM  I LG
Sbjct:  662 YLAYGFSSCIVNGE----QRPQCAIFYEVLANESMKPAKLRKYVATKHESYQDKPLAFFKRKEKVLNSQKSLSHN----IIYDSKLLRASFEISYLIAKDKMPCTIAEMLLKPAAMKMCEILLG 777

HSP 2 Score: 30.0314 bits (66), Expect = 3.530e-7
Identity = 18/46 (39.13%), Postives = 32/46 (69.57%), Query Frame = 2 
Query: 1028 MGDLSSEISNQFIADIRASSLKIPLQLEKTADVTNCSQLISLLKDI 1165
            +G+++++   Q +  I+ SS K  +QLE+T DVTN +QL  L++D+
Sbjct:  798 IGEINTDFFIQLLDRIKKSS-KFAIQLEETNDVTNFAQL-QLVEDL 841
The following BLAST results are available for this feature:
BLAST of hypothetical protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZBED81.885e-1432.23zinc finger BED-type containing 8 [Source:HGNC Sym... [more]
ZBED81.885e-1432.23zinc finger BED-type containing 8 [Source:HGNC Sym... [more]
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BLAST of hypothetical protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|A4Z945|ZBED8_BOVIN1.507e-1434.75Protein ZBED8 OS=Bos taurus OX=9913 GN=ZBED8 PE=2 ... [more]
sp|Q8IZ13|ZBED8_HUMAN8.386e-1432.23Protein ZBED8 OS=Homo sapiens OX=9606 GN=ZBED8 PE=... [more]
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BLAST of hypothetical protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZIG41.050e-145100.00Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
T1FCQ42.554e-1836.23Uncharacterized protein OS=Helobdella robusta OX=6... [more]
T1EPU12.691e-1836.23Uncharacterized protein OS=Helobdella robusta OX=6... [more]
T1FGE52.994e-1836.23Uncharacterized protein OS=Helobdella robusta OX=6... [more]
T1F4F54.288e-1735.42Uncharacterized protein OS=Helobdella robusta OX=6... [more]
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BLAST of hypothetical protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hypothetical protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000032191.12.255e-1631.45pep primary_assembly:ASM223467v1:7:34400406:344015... [more]
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BLAST of hypothetical protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000019707.11.886e-151100.00SMESG000019707.1[more]
SMESG000067084.19.086e-1233.33SMESG000067084.1[more]
SMESG000028317.13.530e-729.03SMESG000028317.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30008690
GenBankAFJ24818.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008690 ID=SMED30008690|Name=hypothetical protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3495bp
CGTATATAAAAGCTAGCGGGTAACAAAACTCGCCGCTGAGGCGCGAATTT
AGTTTGACGAAATAAACCACTTATGAGGTTAATATTGTCAAACACCAAAT
TAAAAAAAATTTACTAAATTGATTATTTACAAAATCCGCAAAATTTTTCC
GGCTTTCAGTGAAAAATTTCACCGTGGAATTTTTAGTTTATCATTCAGAA
AACGTAATTTTTGGAACTTTATCGATTGATTTCAGCCGAAATATTAATCA
GAAAAATTTAGCCAGGCTGCTTTATCTTTCATCAGTACTTCATTGTAAGT
CGGGTCATTATCTCAATAACGTTCGTTATAGTGCAGGGTTTTCTACCAAG
AAATGCGTAAAAGCATTCCAGGTGGCGCGAGGTAACATCAGTTTTTTTGC
TTATATTAAAGAAAAGAATATCGATTTTCTAAAGTCCAGATTATAAATCG
GGATGCCGTATTTTTCAATGGAATGACATCAACATCTGTTTCATTTATAT
GGCATCGCCATATAATTGTCTGTAATCGGAACATTTAAAATAGGAGTCAA
ACTTGTCGTTTCGTGTTTATAGCCAATTACGTTCGATTTTAATTTTTTAT
TATTTGTATTTTGTATTCACATGATTGACTTGCACCGGAAAAATTGGGGA
TCTGCAAAAACCCCACTGGAAAATTATTGATGCTGTCATTTCAAACACAC
ATCTAAAGCCATTTAAGTTAATGAAACAAATCAATTCAGGCATGGTGGAG
CAGATGTTGCAGATCATGATGTTTAATCAATGAAACCTTAAAAGAGTCCA
CTTTGATTCTCGAGCAACACATCAAAATTCAGCTCTTATTTCTTCTGAAA
AATCACTGTAGATGGCTTAATACCACATCGACTATAAAGTAGCCAAATCA
AAGGAGCCCCATGTAATTACAGAAAACATGATAAAACCATGTGCATTAAA
AATGAATGCAATTTTTCTAGGTAAATAAACATAATCTTTAATTGGTAGTG
TTATAAATTGCCTGTTATTTAAAGCTGATGGGTGATTTGAGTTCGGAAAT
TTCGAACCAATTTATTGCAGATATCAGAGCTAGTTCCCTAAAGATCCCTC
TTCAGTTGGAGAAAACAGCTGATGTCACAAATTGCAGTCAACTAATCTCA
TTACTGAAAGACATTTATGATGGTGCCATATAGAACATAATGGCTTACAT
TTTCAATCAATTGAGTTTATGAATGGATAGAAAAGAGCACAATATGTAGA
TCGACTAATAATGAAATTTATATATCCTAATCAGTTTTTAAGTTAAACAA
CTCTTGATGTCAATTTTCAGGCTAACCTCAAGTCGCCAGCTGCAGCCGTA
TTAGCTAGGTCTTTGCCAGACTATATTATGCATTGATATTTAACAAATTT
CCGATTATTAAGAAAAATTCTGCAATGAAACAAATGGTTTCTGGTGCGGT
GACCTCTGTATTGATGTTAAACTTCGTTGTGATGGACGAGAAGACTGTAA
AATATTTTCTAACTCAAATTTGGAGTCGGCTGATGAAATGAATTGTACAA
AATACAATAGAAGTTTCGGATATATCATCCCCGAAAATTTCCGTCTAAAA
AGTCCAAGTGTTCTGTTGATAAAAACGCAAAGTTGTGGCATAATAATCTT
ACTCGAACAAAGAAAGTTAAACGAAGGAGTGATGTGAACTTTGTGGACAA
GAATTTGTCAGATTTTGTAAAGTTACCTTTGAAGAAGGATGTAATTGGAA
GATTTTTATTTTTATACGAAGAAAAAAAGGATAGAAGTTGTAGAATAAAG
AAAATTTGTAATAAATTAGTGACTTTGTGGTCTAAATTCAACTTTCCGTC
CCTCAGTCATCAAGTAGTAATTGCAAAAATAAAGAAAATTTTGAATCAAT
ACGATAACCACAGGAAAATGCAGTCGCAAAGTTAGTATATGAACTGCTAG
CAATTTTTATATCACCAAGCATGATGGACTGTTGTTGTGCCGAGAAGATA
AGGATTTGTTAACAGTTGTAAATAAAACGGGATTGTGACCAAAATTAAAC
CCGAGATGGTTAAAAAAGGTTTTCAAAAACCTCAATACATCGGTTGTCAA
CACCATAATTTAGATAGAATCTTGAAACACGTATGGGATTATTTCATCTC
AAATCATTTCCGAAAACCTACATTAAATTATGCATTCATAGATGAAATTA
CCAAAGATTCGGTAGAACTGCAGAAACTGTATAGAGGAGAAGCTCAAATC
AAGGAAAGTAAAAATACTGGATGGCGAGACAATTTAAAATTTTTGTTCGG
ATTATGTGATGCCTTCAAGTTATACAGGAAATGTGAAAATCTTCCCTTGA
TTAAATTGCGAAAATTGCAATCTCTACATGCAGCAAGTTGGAATTCGAGA
GCCTTGTAGCATACTTGATGGTTGTTGATAGAGAATATCAATCAATTTAC
AGAATAGTATTCAAACTGTGTATGACTTCTCTTCAAATCAGTTATCAAAC
AACTAATCAAAATGATCATCAAGCTAATCACCATACTGATCATCAACATG
TTTATCAAATTGCTCATATTTATCAAACTTTTCATTCATACTTAAGTCGA
TTCTTCGGACCAATCGTTTTGGATCAGAAATTTATTTTATATAATATTAA
GTATCATCGGGCTTATCTTAATATTCTCGGCAATAAAAATTTATATTACA
AATTTTGTGATTTCTAAACATCGTCGAACAACAAAGAAGTCTTCCACACG
CGATTCTCCACTCTATTCATTGTCACTTGGAGATTCTGACGTCGAATCAG
CTTACGATGATATAGAAAAATGTAGTGAAACGGAATCAAAGAAAAATTCT
TCATTAATTATTAGTGAAGATGAAGAAACTCAAGAGGAAATTTTAGGAAC
GTCGAAAGAATATCTCGATTCAGTTTATAATAACAATGTATTTCATCCTC
AATTAGTCAGTCCAAGAAAACAATGTGTTGAAAAGAAAAGCCATACCGTG
CCTGTTCCAGTAGAATTGCATTCTTTATCGTCTTATAAACTTCAAGATTG
CCCAACAAATATAATGACATCTAATAGTATTTCTCTCACTGGAACTATGT
TATCTCAACGACACAATGCCATATCTGAGATTAGCTCTCCTGGATCTTCA
AGAACATTTCGGAAAATATTCAAAGAAAACCTCAACAAAACATCAAGTTA
TGATAAAATTATCACGTCTAATGAAGATTCATTCAGGACTTCTCGTGATA
TAGAGTCTATTAAAACTACTCCATCAATAATAAAGCCCAAAAGTCACACA
ATATCGAAGTTGGAAAACAATGACAGAGATAATGATAATTTCACCGCTTA
CGTAATCATTTAATCGAGCCTTTTGGAGGAATCTGTGATAACTTATTTAT
TTTCATGGCTGTATTTATATTTATGTCATCATTTTTAAATATTATCGAAT
TCTATCAAAATTCGAATTTGTTTTTTACTTATTTTTAGTTTTCTA
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protein sequence of SMED30008690-orf-1

>SMED30008690-orf-1 ID=SMED30008690-orf-1|Name=SMED30008690-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MTSNSISLTGTMLSQRHNAISEISSPGSSRTFRKIFKENLNKTSSYDKII
TSNEDSFRTSRDIESIKTTPSIIKPKSHTISKLENNDRDNDNFTAYVII*
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protein sequence of SMED30008690-orf-2

>SMED30008690-orf-2 ID=SMED30008690-orf-2|Name=SMED30008690-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
MVKKGFQKPQYIGCQHHNLDRILKHVWDYFISNHFRKPTLNYAFIDEITK
DSVELQKLYRGEAQIKESKNTGWRDNLKFLFGLCDAFKLYRKCENLPLIK
LRKLQSLHAASWNSRAL*
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protein sequence of SMED30008690-orf-3

>SMED30008690-orf-3 ID=SMED30008690-orf-3|Name=SMED30008690-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MVVDREYQSIYRIVFKLCMTSLQISYQTTNQNDHQANHHTDHQHVYQIAH
IYQTFHSYLSRFFGPIVLDQKFILYNIKYHRAYLNILGNKNLYYKFCDF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0004672protein kinase activity
GO:0004674protein serine/threonine kinase activity
GO:0004675transmembrane receptor protein serine/threonine kinase activity
GO:0005524ATP binding
GO:0016301kinase activity
GO:0016740transferase activity
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0007178transmembrane receptor protein serine/threonine kinase signaling pathway
GO:0016310phosphorylation
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005654nucleoplasm
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR002172LDrepeatLR_classA_rpt