SMED30008680

Overview
NameSMED30008680
Smed IDSMED30008680
Length (bp)1523
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008680 (SMED30008680) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008680 (SMED30008680) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008680 (SMED30008680) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008680 (SMED30008680) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008680

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008680SMESG000063962.1 SmedASXL_011286SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30008680SMESG000063962.1 dd_Smed_v4_18803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008680SMESG000063962.1 dd_Smed_v4_18803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30008680SMESG000063962.1 dd_Smed_v4_18803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008680SMESG000063962.1 Contig3462newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008680SMESG000063962.1 Contig3462uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30008680SMESG000063962.1 dd_Smed_v6_18803_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30008680SMESG000063962.1 OX_Smed_1.0.11968ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008680 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.015619:4678..6198 -7455
Homology
BLAST of SMED30008680 vs. TrEMBL
Match: A0A267GCJ6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031497g1 PE=4 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 6.193e-89
Identity = 152/485 (31.34%), Postives = 252/485 (51.96%), Query Frame = 1
Query:   40 HLKKMGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFER---------DSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGI--HNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCN---EYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFS 1452
            H   MGN YS+I    +P   F++ FRQPD +  +NAE  L+  L Q     W  G+++EKK   G+EL+L+  PF ++  P ++L+    L+  +L  +L  G  +++SG +++  ERS  + RK +         + ++ +E W ++++ N     S+ L+ ++  +    +  +     Y  ME+  QPWDS+KS S +SPLI L +  LL+  GY  +   H  G  D + F +         D    V++  +FVLSLNQ DQLR Y A  +    + +     WE G+     S  D +    L  SPW V +WDKD++F ++  + E+ +EL    W+I +   +YRS YDKSL V +RNS      H  S+  HD++RLC+ G+ + L ++L +   + W +G + +++E+      E+  + KPW    E+   NL+  G     ++ +   GW +  + DICS+S +SP+  I S
Sbjct:   41 HAAAMGNDYSRIQRRAFPQSAFVVAFRQPDHLLVLNAEPALLQQLRQAVSANWRGGVRSEKKYPLGVELRLDKEPFDSEVHPADRLVPIYRLVTALLKLLLDRGCTLLASGAVVRGVERSAWLLRKASDLCPARDVELLTVAMEDWGKLLLINSPAALSTALMAKLKEVWPKGVELVSEEPTYFAMELGEQPWDSYKSASERSPLIVLEVTRLLLAHGYSFLAATHARGSMDCLTFVKSTFPASDCQDEQPAVDDG-LFVLSLNQRDQLRLYAAPQEVRECVEKFAATSWESGVLRSEASAGDLLQSVQLKSSPWFVPDWDKDAAFCAREFIQELLRELRSKGWQIAVKADIYRSAYDKSLLVLRRNSAQQSLPH-LSVTTHDVNRLCVLGATESLKAELVNDVKDAWSRGHRCEAFESASGWTVEFSFDRKPWQWDPEAPLTNLEGFGLVATLIDVLQQRGWHVETSLDICSSSKFSPSTIILS 523          
BLAST of SMED30008680 vs. TrEMBL
Match: A0A267E9A1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005544g1 PE=4 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 1.382e-88
Identity = 152/485 (31.34%), Postives = 252/485 (51.96%), Query Frame = 1
Query:   40 HLKKMGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFER---------DSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGI--HNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCN---EYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFS 1452
            H   MGN YS+I    +P   F++ FRQPD +  +NAE  L+  L Q     W  G+++EKK   G+EL+L+  PF ++  P ++L+    L+  +L  +L  G  +++SG +++  ERS  + RK +         + ++ +E W ++++ N     S+ L+ ++  +    +  +     Y  ME+  QPWDS+KS S +SPLI L +  LL+  GY  +   H  G  D + F +         D    V++  +FVLSLNQ DQLR Y A  +    + +     WE G+     S  D +    L  SPW V +WDKD++F ++  + E+ +EL    W+I +   +YRS YDKSL V +RNS      H  S+  HD++RLC+ G+ + L ++L +   + W +G + +++E+      E+  + KPW    E+   NL+  G     ++ +   GW +  + DICS+S +SP+  I S
Sbjct:   69 HAAAMGNDYSRIQRRAFPQSAFVVAFRQPDHLLVLNAEPALLQQLRQAVSANWRGGVRSEKKYPLGVELRLDKEPFDSEVHPADRLVPIYRLVTALLKLLLDRGCTLLASGAVVRGVERSAWLLRKASDLCPARDVELLTVAMEDWGKLLLINSPAALSTALMAKLKEVWPKGVELVSEEPTYFAMELGEQPWDSYKSASERSPLIVLEVTRLLLAHGYSFLAATHARGSMDCLTFVKSTFPASDCQDEQPAVDDG-LFVLSLNQRDQLRLYAAPQEVRECVEKFAATSWESGVLRSEASAGDLLQSVQLKSSPWFVPDWDKDAAFCAREFIQELLRELRSKGWQIAVKADIYRSAYDKSLLVLRRNSAQQSLPH-LSVTTHDVNRLCVLGATESLKAELVNDVKDAWSRGHRCEAFESASGWTVEFSFDRKPWQWDPEAPLTNLEGFGLVATLIDVLQQRGWHVETSLDICSSSKFSPSTIILS 551          
BLAST of SMED30008680 vs. TrEMBL
Match: A0A267DV85 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034140g1 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 1.993e-88
Identity = 152/485 (31.34%), Postives = 251/485 (51.75%), Query Frame = 1
Query:   40 HLKKMGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFER---------DSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGI--HNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCN---EYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFS 1452
            H   MGN YS+I    +P   F++ FRQPD +  +NAE  L+  L Q     W  G++ EKK   G+EL+L+  PF ++  P ++L+    L+  +L  +L  G  +++SG +++  ERS  + RK +         + ++ +E W ++++ N     S+ L+ ++  +    +  +     Y  ME+  QPWDS+KS S +SPLI L +  LL+  GY  +   H  G  D + F +         D    V++  +FVLSLNQ DQLR Y A  +    + +     WE G+     S  D +    L  SPW V +WDKD++F ++  + E+ +EL    W+I +   +YRS YDKSL V +RNS      H  S+  HD++RLC+ G+ + L ++L +   + W +G + +++E+      E+  + KPW    E+   NL+  G     ++ +   GW +  + DICS+S +SP+  I S
Sbjct:   41 HAAAMGNDYSRIQRRAFPQSAFVVAFRQPDHLLVLNAEPALLQQLRQAVSANWRSGVRLEKKYPLGVELRLDKEPFDSEVHPADRLVPIYRLVTALLKLLLDRGCTLLASGAVVRGVERSAWLLRKASDLCPARDVELLTVAMEDWGKLLLINSPAALSTALMAKLKEVWPKGVELVSEEPTYFAMELGEQPWDSYKSASERSPLIVLEVTRLLLAHGYSFLAATHARGSMDCLTFVKSTFPASDCQDEQPAVDDG-LFVLSLNQRDQLRLYAAPQEVRECVEKFAATSWESGVLRSEASAGDLLQSVQLKSSPWFVPDWDKDAAFCAREFIQELLRELRSKGWQIAVKADIYRSAYDKSLLVLRRNSAQQSLPH-LSVTTHDVNRLCVLGATESLKAELVNDVKDAWSRGHRCEAFESASGWTVEFSFDRKPWQWDPEAPLTNLEGFGLVATLIDVLQQRGWHVETSLDICSSSKFSPSTIILS 523          
BLAST of SMED30008680 vs. TrEMBL
Match: A0A0M3JRI7 (Uncharacterized protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS10081 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.307e-31
Identity = 115/470 (24.47%), Postives = 214/470 (45.53%), Query Frame = 1
Query:   52 MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGI-ELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKL---NVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKS-NSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFF--ERDSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQ--DKIWICY-LNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWAL-----EHISDFGWSLVETADICS 1416
            MG + S          YF L F   D I  ++A  ++I  +  V  +    GI++E +   G  + KL   PF    + +   +QT L++CE+L  +   G+ +  S  + +  + STL F+K+     E       I  + L   +R+ + N     ++ L++ + +L   +     S  NY  ++M G  W++  +    ++  + LN      +  Y+    A++ G  D +FF  +RD    V + +  +LSLN  D+LR  CA ++ ++ +   ++  W KGI +  ++  D +   Y L G PW   N     +  S+LL+  I ++L    W +  +I L R   DK++F+ +R + +   +  F+I P + D+L +  + K ++  + +     W  GI+ + Y     EY + G PW  T   SS+  D     C  +     + +   GW ++ +AD+ S
Sbjct:    1 MGQQVSSFPKRPKDFTYFCLTFHVTDSIVLVDASEEVISMVRSVVSQLSAAGIQSESQGPCGAYKFKLRGMPFVTSSREIS--VQTKLMLCELLKVLRENGWELTLSSDLSRRRDLSTLFFQKIPNNGYEYAFRGCHIMCLSLSSMDRLQLVNAPVEANNILIECVDTLHQRD----HSGQNYFEVQMKGYLWEALTAEGGFQARSLLLNTFSRFHELRYRFYGTANIKGTADCIFFISDRDD---VGQREYCMLSLNASDRLRLVCAPNEVVDLVGECLQRYWVKGIQHAKVKGGDPLHFEYKLGGFPW---NSMGTEAVESRLLITIILQQLVTIGWAVVTAIDLSRRATDKAVFLLRRCAASTIPH--FAICPAETDKLRVINANKEMIEVVGNVLRSCWTLGIQREEYYGNSFEYKLVGYPWSST--VSSLTGDHQYELCRMMMISLIDELGRHGWRVICSADVSS 454          
BLAST of SMED30008680 vs. TrEMBL
Match: A0A267FU33 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033074g3 PE=4 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 8.436e-31
Identity = 111/475 (23.37%), Postives = 217/475 (45.68%), Query Frame = 1
Query:   52 MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILS-LQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFERDSGT-------LVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKDS-SFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICST--SAYSPTDY 1443
            MG  YS + ++   +++F + FR PD I+ I+ +   ++ + +   R +  G++ E          L  NPF       E+ +    + C +L ++   G+ +++S  + ++ + S+  F + N     + S++C I L G+++  + N  +   + +   +     + I   ++     + M+M+G PW + +  +V++  + L +I  +   GY +     +    D++FF   S         L + +  FVLSLN+ D+LR   A S+A   +  V+   W  G+  D          L GSPW    W + + S  ++LL+  + +EL    W +  +I+L R   DKS+FVF R    +  +   S+  H    L    +P  ++  L+   + +++ GIK +    G ++  + G+PW  +  + + ++         LE +   GWSLV +AD+ +    A S  DY
Sbjct:    1 MGGNYSYLPSEPASNNFFCITFRVPDSIKVIDCDPPTLEIVRKAIARGYRLGLRQEYDKYGSRTFVLAGNPFKVGCSK-EEAIAGKQVACTLLQDLAHKGWQVLNSSELCRNKDLSSWFFERTNEACVNYDSTVCCIALSGFDKFQLVNFPKQLLAKVADAVRRTWTLGIQEEMKDAYGSMEMKMNGNPWSASERGAVEARRMILEIIRAMASEGYVLCMSTSIKDTTDSLFFRSASAADQPSCSELGSPDASFVLSLNRHDRLRLIEAPSEAQEVVRNVLLHHWTSGLTGDRDYHGSREFKLGGSPW----WTEGAESVRTRLLITLLLQELRDKGWRLVQTIELCRRLNDKSVFVFARAQPESSLHWCLSL--HSKSVLRFVNAPVEVVDALRLVIAGNYIHGIKTERVFAGYHQVRLRGRPW--SSHNGNDHVHGRHMMLGILEAMQQRGWSLVCSADVSAIYHHADSGQDY 466          
BLAST of SMED30008680 vs. Ensembl Nematostella
Match: EDO36791 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHJ7])

HSP 1 Score: 85.5001 bits (210), Expect = 9.583e-18
Identity = 100/489 (20.45%), Postives = 196/489 (40.08%), Query Frame = 1
Query:    7 HFMFNNT*INFHLKKMGNKYSQISTD----LYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRK---LNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLL-MQCGYKVMCKAHVTGYNDTVFFERDSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIK--LQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKD--SSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIK-EDSYENGCN--EYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAY 1428
             +M ++T    + K MG   S+   +      P +Y  +   +P  I  IN   D    + +     W  GIK E   + G    L   PF  + +      +   ++ +I+ ++   G+ +     + + F  S   F +    + EL     S+ S+     + I+  NI     + + + I       I   +   N L +++ G+PW   K  S+ + L+   L  L   +   ++ C+ ++    D+  F        +EN    + L     +    A S+AI  + +V++    W  GI ++      +   L GSPW    W +    S  S+L++  + +EL  ++W+I       +   +K + VFQ+    N ++    + P   ++L +  +   + + +++    HW   IK E  Y       E+ + GKPW      ++ +  A    C+ ++ +   GW L  +ADI +   Y
Sbjct:    5 RYMLSHTHSAINSKTMGTSDSRQGKESDELQSPPNYLCVTAVKPATIHLINGGKDEQIIIQEAITESWEQGIKDETPQKHGHSWTLQEFPFGYNKQGFYCPAREIRVLSKIIQSLDKNGWLLAGVADLSRRFVFSQWFFIRGSGQHSELDYPCVSLSSV-----DTILFVNIPSQFYARIKELILKYWDKGIKESKEEENTLELQLKGKPWHG-KILSMPARLLMTQLFGLFHRKYNLELYCRLNLRCKADSFLFRPKVKESKDENTFMGICLYGKSGVLIIDAQSEAITIVRKVLQETWMWRGGIKDEKEYVACYGFTLAGSPW----WSRKRKQSAMSRLVICRLIEELQSSSWKIITGAAFSKHSMNKDMLVFQKCKPMNVSS--MCLCPVGANKLRLVNATSEIFATVRELIQTHWPTRIKRERRYRTPAAFLEFKLNGKPW---SGKTTDSFSASNMMCYIMQELQRQGWKLALSADIAAKYMY 478          
BLAST of SMED30008680 vs. Ensembl Nematostella
Match: EDO30470 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0Q1])

HSP 1 Score: 69.3218 bits (168), Expect = 1.231e-13
Identity = 51/217 (23.50%), Postives = 96/217 (44.24%), Query Frame = 1
Query:  793 LSLNQLDQLRTYCATSDAINAISRVIKLQW--EKGIHNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGP------HDIDRLCITGSPKCLLSQLKDRFSEHWVKGIK-EDSYENGCNEYIME--GKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADI 1410
            +SLN+ D+LR   A    I  + +++   W    GI  +      W   L+G+PW       D +  S+ +M +I + L    W +  ++ + R Q DKS+  F ++           I P      +D  ++ +  +P+  +   +D     W+KGI+ E +    C  Y  +  G PW        +++ ++   C+ L+ ++  GW L+ +ADI
Sbjct:    1 ISLNRNDRLRLINAPQPIIETVKQILSQYWSTRGGIQKERQYHASWEFKLSGTPWWACG---DEAVMSRFVMCKILEGLQSQGWHVRAALDMCRRQNDKSVLAFYQS--------LPKIAPVVCLSFNDECKIRLINAPQEFVGLCRDIIQARWIKGIRDEKALNTPCMAYQFKLFGNPWSGYSIVDGLHIRSM--LCFILQMLASRGWKLLISADI 204          
BLAST of SMED30008680 vs. Ensembl Nematostella
Match: EDO45093 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTU8])

HSP 1 Score: 62.003 bits (149), Expect = 8.597e-11
Identity = 50/219 (22.83%), Postives = 94/219 (42.92%), Query Frame = 1
Query:  784 VFVLSLNQLDQLR--TYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKDS-SFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYEN-----GCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICS 1416
            +F +SLN  D +R   Y A   ++     V    W  G+ ++      +   LNG+PW    W ++  S  ++ L++++ + L    W +     + R    KS  +FQ+++          I     D+L    +P   LS +++   +HW K I  +  ++      C ++ M G PWH  ++       A    C+ L+ +S   W  V +AD+ +
Sbjct:    1 MFSISLNGTDGVRLINYPANMQSVVQQVLVDNWAWRGGVASEREFYGGYEYKLNGNPW----WSEEKESIMARYLILKLLEALQGYGWHVIGGFDVSRRLTTKSTLIFQQSAPM--KTPLLCISASGSDKLRFINTPDAELSIMREIMHKHWPKKISAEKRKDTALGVSCVQFKMNGTPWHGEDDDKYY---ARSMMCFLLQALSKRSWKPVLSADVSA 210          
BLAST of SMED30008680 vs. Planmine SMEST
Match: SMESG000063962.1 (SMESG000063962.1)

HSP 1 Score: 956.051 bits (2470), Expect = 0.000e+0
Identity = 466/469 (99.36%), Postives = 468/469 (99.79%), Query Frame = 1
Query:   52 MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFERDSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFSKV 1458
            MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRII+NNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPL+FLNLICLLMQCGYKVMCKAHVTGYNDTVFFERDSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPK LLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFSKV
Sbjct:    1 MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWPFGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCGYGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIVNNIDEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSPLVFLNLICLLMQCGYKVMCKAHVTGYNDTVFFERDSGTLVNENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNNDNNHFFSIGPHDIDRLCITGSPKGLLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFSKV 469          
BLAST of SMED30008680 vs. Planmine SMEST
Match: SMESG000064344.1 (SMESG000064344.1)

HSP 1 Score: 50.0618 bits (118), Expect = 3.044e-6
Identity = 63/294 (21.43%), Postives = 118/294 (40.14%), Query Frame = 1
Query:  622 SHKSNSVKSPLIFLNLICLLMQCGYKVMCKAHVT---GYNDTVFFERDSGTLVN----ENDVFVLSLNQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDS-IQDKI--WICYLNGSPWSVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQRNSLNND-----NNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKEDSYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVETADICSTSAYSPTDYIFSKV 1458
            S +S+S K  L+F     L  + G+ ++    +T     +   +F+ D+  L++    EN+   + L+  D  +      +  N I   I+  W KG  N   I+D +  W   LNG PWS        S  ++ L++ + + +S +NW +  S  + +   D   F  Q N LN D       ++F +   +   L I G    L   + +   ++  KG K    ++      +  K W    E  +   +         + ++  GW   E+ D C+      +  +F++V
Sbjct:   76 SFQSDSAKFKLMFCKFHKLFYKHGWSLVSSPTLTFSVSLSSWFYFKYDNPFLLHDKKVENEFCAIGLSNTDGFQFINFPIELENPIINCIRQNWTKGTKNKKLIKDTVNLWSLQLNGFPWSCYG---KQSVDARYLVLCLIRFMSHHNWRLVNSTAMKQDASDTMFFRSQSNMLNVDCPESMPEYYFMLALINPGSLSILGCDANLGQFIANSCDKYETKGSKLLYTKHNYVSLDLNSKVWSRRNEKET---ETRRLMTCLFQDLAAIGWIPTESID-CTRHITDLSSILFTRV 362          
The following BLAST results are available for this feature:
BLAST of SMED30008680 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GCJ66.193e-8931.34Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267E9A11.382e-8831.34Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267DV851.993e-8831.34Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A0M3JRI71.307e-3124.47Uncharacterized protein OS=Anisakis simplex OX=626... [more]
A0A267FU338.436e-3123.37Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30008680 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO367919.583e-1820.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO304701.231e-1323.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO450938.597e-1122.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30008680 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008680 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000063962.10.000e+099.36SMESG000063962.1[more]
SMESG000064344.13.044e-621.43SMESG000064344.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008680 ID=SMED30008680|Name=SMED30008680|organism=Schmidtea mediterranea sexual|type=transcript|length=1523bp
ATTTTGCATTTTATGTTCAATAATACTTGAATCAATTTTCATCTCAAAAA
AATGGGAAATAAATATTCACAAATCTCCACAGATCTTTATCCTAGTCATT
ATTTTATTTTGATATTTCGTCAACCGGATATTATTCAGGCTATCAATGCA
GAAAGTGATTTGATAGATAATTTGACTCAAGTCTTTGAAAGGTTCTGGCC
ATTTGGCATTAAAACGGAAAAGAAAATTCGATTCGGCATCGAGTTGAAAT
TAAATAACAATCCGTTTTCCAATGATCCGAAGCCGCTGGAAAAATTGCTA
CAAACCAATCTATTGATATGTGAGATTTTGTCCAATGTCCTTAGCTGTGG
ATATGGCATCGTATCTTCTGGATATATTATCAAGTCGTTCGAGAGGTCTA
CTTTAATTTTTCGAAAATTGAATGTCGAATTAAAGGTCCACTCATCAAGC
ATCTGTTCAATTATTCTCGAAGGCTGGAATCGGATTATCATTAATAACAT
TGATGAAATGACATCAAGCTTTTTGTTAAAGGAAATAACTTCATTAGCAA
TGATTAACATTTTAAGTTTACAATCAATTAGCAATTATTTAACTATGGAA
ATGGATGGCCAGCCATGGGATTCGCATAAATCTAATAGTGTGAAATCGCC
TCTCATATTTTTGAATCTAATTTGTCTGTTGATGCAGTGCGGATATAAAG
TAATGTGCAAAGCCCATGTCACCGGATATAATGATACAGTATTTTTCGAA
CGAGATTCCGGCACATTAGTTAATGAAAATGACGTTTTCGTGCTTAGTTT
AAATCAACTTGATCAACTGAGAACATACTGTGCTACCTCGGATGCCATAA
ATGCCATTTCTCGGGTCATTAAATTGCAATGGGAAAAAGGAATCCACAAT
GATTCGATTCAAGATAAAATATGGATTTGTTATCTTAATGGATCACCGTG
GTCGGTTGCAAATTGGGATAAAGATTCGAGTTTCTCATCTAAACTGTTGA
TGATGGAAATATTTAAGGAACTTAGCAAAAACAATTGGGAAATTACACTT
TCTATTAAACTTTATCGAAGTCAGTATGATAAAAGCCTGTTTGTATTTCA
GAGAAACTCATTGAATAATGACAACAATCATTTTTTTTCTATTGGTCCGC
ATGACATTGATAGACTCTGTATTACTGGTTCTCCAAAATGTTTACTATCT
CAATTAAAAGATCGATTTTCGGAGCATTGGGTTAAGGGTATTAAAGAAGA
CAGTTACGAAAATGGATGCAACGAATACATTATGGAAGGGAAACCTTGGC
ATTTAACTGAAGAAAGTTCAAGCGTTAATTTAGATGCGATTGGTTTCTTT
TGCTGGGCTTTGGAACATATAAGTGATTTTGGTTGGTCATTAGTTGAAAC
GGCCGATATTTGTTCCACAAGTGCTTATTCACCCACTGATTATATATTTT
CAAAGGTTTAATTGTTATTAACTTTACTTTTGATTTTACACTAAATTTAC
TGACAATAAAGTTTTCACAGAAG
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protein sequence of SMED30008680-orf-1

>SMED30008680-orf-1 ID=SMED30008680-orf-1|Name=SMED30008680-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=470bp
MGNKYSQISTDLYPSHYFILIFRQPDIIQAINAESDLIDNLTQVFERFWP
FGIKTEKKIRFGIELKLNNNPFSNDPKPLEKLLQTNLLICEILSNVLSCG
YGIVSSGYIIKSFERSTLIFRKLNVELKVHSSSICSIILEGWNRIIINNI
DEMTSSFLLKEITSLAMINILSLQSISNYLTMEMDGQPWDSHKSNSVKSP
LIFLNLICLLMQCGYKVMCKAHVTGYNDTVFFERDSGTLVNENDVFVLSL
NQLDQLRTYCATSDAINAISRVIKLQWEKGIHNDSIQDKIWICYLNGSPW
SVANWDKDSSFSSKLLMMEIFKELSKNNWEITLSIKLYRSQYDKSLFVFQ
RNSLNNDNNHFFSIGPHDIDRLCITGSPKCLLSQLKDRFSEHWVKGIKED
SYENGCNEYIMEGKPWHLTEESSSVNLDAIGFFCWALEHISDFGWSLVET
ADICSTSAYSPTDYIFSKV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR38696FAMILY NOT NAMEDcoord: 231..454
coord: 16..149