SMED30008638

Overview


Name	SMED30008638
Smed ID	SMED30008638
Length (bp)	1813

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30008638 (SMED30008638) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008638 (SMED30008638) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008638 (SMED30008638) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008638 (SMED30008638) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30008638

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
protonephridia	SMED30008638	SMESG000013006.1	dd_Smed_v4_6779_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30008638	SMESG000013006.1	dd_Smed_v4_6779_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30008638	SMESG000013006.1	dd_Smed_v4_6779_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30008638	SMESG000013006.1	Contig52703	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30008638	SMESG000013006.1	Contig52703	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
epidermis	SMED30008638	SMESG000013006.1	dd_Smed_v4_6779_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30008638 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.006391:10619..12454 -	7540

Homology

[Close]

BLAST of SMED30008638 vs. Ensembl Human
Match: ANKRD17 (ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:HGNC:23575])

HSP 1 Score: 59.3066 bits (142), Expect = 2.062e-8
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1451 NGKLTVASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1543

[Close]

BLAST of SMED30008638 vs. Ensembl Human
Match: ANKRD17 (ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:HGNC:23575])

HSP 1 Score: 58.9214 bits (141), Expect = 2.795e-8
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1586 NGKLTVASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1678

[Close]

BLAST of SMED30008638 vs. Ensembl Human
Match: ANKRD17 (ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:HGNC:23575])

HSP 1 Score: 58.9214 bits (141), Expect = 2.819e-8
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1589 NGKLTVASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1681

[Close]

BLAST of SMED30008638 vs. Ensembl Human
Match: ANKRD17 (ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:HGNC:23575])

HSP 1 Score: 58.9214 bits (141), Expect = 2.856e-8
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1702 NGKLTVASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1794

[Close]

BLAST of SMED30008638 vs. Ensembl Human
Match: ANKHD1 (ankyrin repeat and KH domain containing 1 [Source:HGNC Symbol;Acc:HGNC:24714])

HSP 1 Score: 53.5286 bits (127), Expect = 1.186e-6
Identity = 31/113 (27.43%), Postives = 61/113 (53.98%), Query Frame = -3

Query:  237 PNKITNKGNTWTHINSSKNVQSN----KIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 563
            PN ++    T +   SS N++ N    K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct:  310 PNSLSTSYKTVSLPLSSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 420

[Close]

BLAST of SMED30008638 vs. Ensembl Celegans
Match: pes-4 (Patterned Expression Site [Source:UniProtKB/TrEMBL;Acc:Q95Y67])

HSP 1 Score: 54.299 bits (129), Expect = 1.749e-7
Identity = 36/130 (27.69%), Postives = 59/130 (45.38%), Query Frame = -3

Query:  180 PNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDS-MANELDERTVHLKGTSEAVHKAREFICD-LVEGKITGNHVIQESETFFDEI 563
            P +I     T   I  + N+  NK EE +      +      + + V     G +IG+GGS +  IR+ TGA I + S M     ER V L GT++A++     +C  L+E    G+ +    +  F+ +
Sbjct:  113 PERIVTITGTLGVIGKAFNMVCNKFEEDMLLLPNSVPKPPITMRVIVPATQCGSLIGKGGSKIKDIREATGASIQVASEMLPHSTERAVTLSGTADAINLCMTQVCQILLEAPPKGSTITYRPKPTFNPL 242

[Close]

BLAST of SMED30008638 vs. Ensembl Fly
Match: mask (gene:FBgn0043884 transcript:FBtr0334942)

HSP 1 Score: 53.5286 bits (127), Expect = 6.170e-7
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 2674 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 2740

[Close]

BLAST of SMED30008638 vs. Ensembl Fly
Match: mask (gene:FBgn0043884 transcript:FBtr0345261)

HSP 1 Score: 53.5286 bits (127), Expect = 6.221e-7
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 2661 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 2727

[Close]

BLAST of SMED30008638 vs. Ensembl Fly
Match: mask (gene:FBgn0043884 transcript:FBtr0305043)

HSP 1 Score: 53.5286 bits (127), Expect = 7.048e-7
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 3048 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 3114

[Close]

BLAST of SMED30008638 vs. Ensembl Fly
Match: mask (gene:FBgn0043884 transcript:FBtr0334943)

HSP 1 Score: 53.1434 bits (126), Expect = 7.608e-7
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 3038 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 3104

[Close]

BLAST of SMED30008638 vs. Ensembl Fly
Match: mask (gene:FBgn0043884 transcript:FBtr0084562)

HSP 1 Score: 53.1434 bits (126), Expect = 7.609e-7
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 3039 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 3105

[Close]

BLAST of SMED30008638 vs. Ensembl Zebrafish
Match: ankhd1 (ankyrin repeat and KH domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-090421-1])

HSP 1 Score: 55.4546 bits (132), Expect = 2.012e-7
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = -3

Query:  237 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 530
            T  NS  N  S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1708 TSPNSKMNPLSPKRTQKREEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1803

[Close]

BLAST of SMED30008638 vs. Ensembl Zebrafish
Match: ankhd1 (ankyrin repeat and KH domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-090421-1])

HSP 1 Score: 55.4546 bits (132), Expect = 2.062e-7
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = -3

Query:  237 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 530
            T  NS  N  S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1681 TSPNSKMNPLSPKRTQKREEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1776

[Close]

BLAST of SMED30008638 vs. Ensembl Xenopus
Match: ANKRD17 (ankyrin repeat domain 17 [Source:NCBI gene;Acc:100127573])

HSP 1 Score: 57.7658 bits (138), Expect = 5.400e-8
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    + S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A   I  L++
Sbjct: 1573 NGKLTIASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATHLINALIK 1665

[Close]

BLAST of SMED30008638 vs. Ensembl Xenopus
Match: ANKRD17 (ankyrin repeat domain 17 [Source:NCBI gene;Acc:100127573])

HSP 1 Score: 57.7658 bits (138), Expect = 5.423e-8
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    + S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A   I  L++
Sbjct: 1561 NGKLTIASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATHLINALIK 1653

[Close]

BLAST of SMED30008638 vs. Ensembl Xenopus
Match: ANKRD17 (ankyrin repeat domain 17 [Source:NCBI gene;Acc:100127573])

HSP 1 Score: 57.7658 bits (138), Expect = 5.618e-8
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    + S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A   I  L++
Sbjct: 1449 NGKLTIASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATHLINALIK 1541

[Close]

BLAST of SMED30008638 vs. Ensembl Xenopus
Match: ANKRD17 (ankyrin repeat domain 17 [Source:NCBI gene;Acc:100127573])

HSP 1 Score: 57.7658 bits (138), Expect = 5.632e-8
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    + S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A   I  L++
Sbjct: 1546 NGKLTIASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATHLINALIK 1638

[Close]

BLAST of SMED30008638 vs. Ensembl Xenopus
Match: ANKRD17 (ankyrin repeat domain 17 [Source:NCBI gene;Acc:100127573])

HSP 1 Score: 57.7658 bits (138), Expect = 5.876e-8
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    + S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A   I  L++
Sbjct: 1552 NGKLTIASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATHLINALIK 1644

[Close]

BLAST of SMED30008638 vs. Ensembl Mouse
Match: Ankrd17 (ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MGI:1932101])

HSP 1 Score: 58.5362 bits (140), Expect = 2.801e-8
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K        W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1447 NGKLTVASPKRGPKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIRECTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1539

[Close]

BLAST of SMED30008638 vs. Ensembl Mouse
Match: Ankrd17 (ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MGI:1932101])

HSP 1 Score: 57.7658 bits (138), Expect = 4.189e-8
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K        W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1697 NGKLTVASPKRGPKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIRECTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1789

[Close]

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K        W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1698 NGKLTVASPKRGPKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIRECTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1790

[Close]

BLAST of SMED30008638 vs. Ensembl Mouse
Match: Ankrd17 (ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MGI:1932101])

HSP 1 Score: 57.7658 bits (138), Expect = 4.428e-8
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K        W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1589 NGKLTVASPKRGPKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIRECTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1681

[Close]

BLAST of SMED30008638 vs. Ensembl Mouse
Match: Ankhd1 (ankyrin repeat and KH domain containing 1 [Source:MGI Symbol;Acc:MGI:1921733])

HSP 1 Score: 51.6026 bits (122), Expect = 2.710e-6
Identity = 23/77 (29.87%), Postives = 47/77 (61.04%), Query Frame = -3

Query:  237 WQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 467
            W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct:  179 WKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 253

[Close]

BLAST of SMED30008638 vs. UniProt/SwissProt
Match: sp|O75179|ANR17_HUMAN (Ankyrin repeat domain-containing protein 17 OS=Homo sapiens OX=9606 GN=ANKRD17 PE=1 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 1.372e-7
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K  +     W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1702 NGKLTVASPKRGQKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIREFTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1794

[Close]

BLAST of SMED30008638 vs. UniProt/SwissProt
Match: sp|Q99NH0|ANR17_MOUSE (Ankyrin repeat domain-containing protein 17 OS=Mus musculus OX=10090 GN=Ankrd17 PE=1 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 2.930e-7
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = -3

Query:  237 NSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 521
            N    V S K        W+++    KK  + V    I +VIG+GG  +NAIR+ TGAHIDID   ++  +R + ++G +E+  +A + I  L++
Sbjct: 1698 NGKLTVASPKRGPKREEGWKEVVRRSKK--VSVPSTVISRVIGRGGCNINAIRECTGAHIDIDKQKDKTGDRIITIRGGTESTRQATQLINALIK 1790

[Close]

BLAST of SMED30008638 vs. UniProt/SwissProt
Match: sp|Q9VCA8|ANKHM_DROME (Ankyrin repeat and KH domain-containing protein mask OS=Drosophila melanogaster OX=7227 GN=mask PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 7.627e-6
Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = -3

Query:  237 KKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 443
            KK+ + VN   I +VIG+GGS +NAIR  TGAHI+++       ER + +KG ++A  +A   I  L++
Sbjct: 3039 KKVQVPVNA--ISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQAHMLILALIK 3105

[Close]

BLAST of SMED30008638 vs. UniProt/SwissProt
Match: sp|Q8IWZ3|ANKH1_HUMAN (Ankyrin repeat and KH domain-containing protein 1 OS=Homo sapiens OX=9606 GN=ANKHD1 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 7.716e-6
Identity = 31/113 (27.43%), Postives = 61/113 (53.98%), Query Frame = -3

Query:  237 PNKITNKGNTWTHINSSKNVQSN----KIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 563
            PN ++    T +   SS N++ N    K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1654 PNSLSTSYKTVSLPLSSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1764

[Close]

BLAST of SMED30008638 vs. TrEMBL
Match: A0A448WVQ0 (KH domain-containing protein (Fragment) OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS14850 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.260e-15
Identity = 42/87 (48.28%), Postives = 62/87 (71.26%), Query Frame = -3

Query:  192 SKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSM-ANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETF 449
            SK+++ IQV++ DIGKVIGQGG+VV+A+R +TG  IDI+S  ++E+ ER V+L+G S+ V +  + I  L+ G ITGN V+     F
Sbjct:  260 SKRRIAIQVSRHDIGKVIGQGGAVVSALRNMTGIQIDIESARSDEVTERMVYLRGPSDGVQRTHDLIQGLISGTITGNDVLSRHAVF 346

[Close]

BLAST of SMED30008638 vs. TrEMBL
Match: A0A0R3XB68 (KH domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS10778 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 3.641e-15
Identity = 42/86 (48.84%), Postives = 62/86 (72.09%), Query Frame = -3

Query:  195 SKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSM-ANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESET 449
            +K+K+ I V+K DIGK+IGQGG+VV+A+R ++G  IDI+S   +E+ ER V+LKG  E VH+  + I DL+ G + GN VI  ++T
Sbjct:  327 AKRKIAISVSKHDIGKIIGQGGAVVSALRNMSGIQIDIESARGDEVTERMVYLKGPGEIVHRTYQMIQDLLSGALAGNEVIARAKT 412

[Close]

BLAST of SMED30008638 vs. TrEMBL
Match: A0A158R6V9 (ANK_REP_REGION domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS976 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.383e-14
Identity = 51/134 (38.06%), Postives = 76/134 (56.72%), Query Frame = -3

Query:  195 TNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGS--------------KKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSM-ANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESET 551
            +  GN W       + +S++   SI S  +   GS              K+K+ I V+K DIGK+IGQGG+VV+A+R ++G  IDI+S   +E+ ER V+LKG  E VH+  + I DL+ G + GN VI  ++T
Sbjct: 1706 STAGNDWLVAQPGSSNRSHRAPTSIVSQTETFSGSDWKTSGSSGSGGSAKRKIAISVSKHDIGKIIGQGGAVVSALRNMSGIQIDIESARGDEVTERMVYLKGPGEIVHRTYQMIQDLLSGALAGNEVIARAKT 1839

[Close]

BLAST of SMED30008638 vs. TrEMBL
Match: A0A068Y3R2 (Ankyrin domain repeat containing protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000508000 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.363e-14
Identity = 50/134 (37.31%), Postives = 77/134 (57.46%), Query Frame = -3

Query:  195 TNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGS--------------KKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSM-ANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESET 551
            +  GN W     + + +S+++  S+ S      GS              K+K+ I V+K DIGK+IGQGG+VV+A+R ++G  IDI+S   +E+ ER V+LKG  E VH+  + I DL+ G + GN VI  ++T
Sbjct: 1718 STAGNDWLVAQPTSSNRSHRVPTSVVSQTDTFSGSDWRTSGSSGSGGSAKRKIAISVSKHDIGKIIGQGGAVVSALRSMSGIQIDIESARGDEVTERMVYLKGPGEIVHRTYQMIQDLLSGVLAGNEVIARAKT 1851

[Close]

BLAST of SMED30008638 vs. TrEMBL
Match: W6UMR4 (Ankyrin repeat domain-containing protein OS=Echinococcus granulosus OX=6210 GN=EGR_10361 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.391e-14
Identity = 50/134 (37.31%), Postives = 77/134 (57.46%), Query Frame = -3

Query:  195 TNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGS--------------KKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSM-ANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESET 551
            +  GN W     + + +S+++  S+ S      GS              K+K+ I V+K DIGK+IGQGG+VV+A+R ++G  IDI+S   +E+ ER V+LKG  E VH+  + I DL+ G + GN VI  ++T
Sbjct: 1814 STAGNDWLVAQPTSSNRSHRVPTSMVSQTDTFSGSDWRTSGSSGSGGSAKRKIAISVSKHDIGKIIGQGGAVVSALRSMSGIQIDIESARGDEVTERMVYLKGPGEIVHRTYQMIQDLLSGVLAGNEVIARAKT 1947

[Close]

BLAST of SMED30008638 vs. Ensembl Cavefish
Match: ankhd1 (ankyrin repeat and KH domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-090421-1])

HSP 1 Score: 54.299 bits (129), Expect = 3.385e-7
Identity = 34/116 (29.31%), Postives = 60/116 (51.72%), Query Frame = -3

Query:  204 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLV-------EGKITGNHVIQE 530
            T  NS  N+ S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L+       E  I  NH+ Q 
Sbjct:   78 TSPNSKMNLTSPKRGQKREDGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRHAVQLINALIQDPAKELEDLIPRNHIRQP 191

[Close]

BLAST of SMED30008638 vs. Ensembl Medaka
Match: ankhd1 (ankyrin repeat and KH domain-containing protein 1 [Source:NCBI gene;Acc:101167550])

HSP 1 Score: 52.7582 bits (125), Expect = 1.172e-6
Identity = 27/98 (27.55%), Postives = 55/98 (56.12%), Query Frame = -3

Query:  237 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 530
            T  N+  ++ S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1722 TSPNTKHSLTSPKRIQKRDEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1817

[Close]

BLAST of SMED30008638 vs. Ensembl Medaka
Match: ankhd1 (ankyrin repeat and KH domain-containing protein 1 [Source:NCBI gene;Acc:101167550])

HSP 1 Score: 52.7582 bits (125), Expect = 1.183e-6
Identity = 27/98 (27.55%), Postives = 55/98 (56.12%), Query Frame = -3

Query:  237 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 530
            T  N+  ++ S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1764 TSPNTKHSLTSPKRIQKRDEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1859

[Close]

BLAST of SMED30008638 vs. Ensembl Medaka
Match: ankhd1 (ankyrin repeat and KH domain-containing protein 1 [Source:NCBI gene;Acc:101167550])

HSP 1 Score: 52.7582 bits (125), Expect = 1.205e-6
Identity = 27/98 (27.55%), Postives = 55/98 (56.12%), Query Frame = -3

Query:  237 THINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVE 530
            T  N+  ++ S K  +     W+++    KKL +  +   + +++G+GG  + AI+ +TGAHID+D   ++  ER + ++G +E+   A + I  L++
Sbjct: 1764 TSPNTKHSLTSPKRIQKRDEGWKEVVRRSKKLSVPASV--VSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQ 1859

[Close]

BLAST of SMED30008638 vs. Planmine SMEST
Match: SMESG000013006.1 (SMESG000013006.1)

HSP 1 Score: 595.89 bits (1535), Expect = 0.000e+0
Identity = 387/427 (90.63%), Postives = 387/427 (90.63%), Query Frame = -3

Query:  189 MLMPKYMPGLILNQRVENNYRSIYSSDLSSFKTVCKNISNMELSYFIDRSQISSNLSIDKCESCRDFTDFFDEAHYNDMDLDLINRCEACKDIILKAKERQEYEAAKNAKDLLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDDIPKSDKQDVSDDNLVLSKSQNDYMEXXXXXXXXKQEKDDIPKSDKQDVSDDNLVLSKSQNDYMENYYLEDLDQKSLXXXXXXXXXXXNSIKPGKQLKVXXXXXXXXXXXXXXXXXXXKMINGVVKVKKEESSNFPKETNGKXXXXXXXXXXXSPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFF 1469
            MLMPKYMPGLILNQRVENNYRSIYSSDLSSFKTVCKNISNMELSYFIDRSQISSNLSIDKCESCRDFTDFFDEAHYNDMDLDLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY                                       MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETF 
Sbjct:    1 MLMPKYMPGLILNQRVENNYRSIYSSDLSSFKTVCKNISNMELSYFIDRSQISSNLSIDKCESCRDFTDFFDEAHYNDMDLDLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY---------------------------------------MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFL 388

HSP 2 Score: 117.087 bits (292), Expect = 7.018e-27
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = -1

Query:   23 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 193
            FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV
Sbjct:  387 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 443

[Close]

BLAST of SMED30008638 vs. Planmine SMEST
Match: SMESG000013006.1 (SMESG000013006.1)

HSP 1 Score: 421.779 bits (1083), Expect = 3.637e-146
Identity = 308/350 (88.00%), Postives = 308/350 (88.00%), Query Frame = -3

Query:  189 DMDLDLINRCEACKDIILKAKERQEYEAAKNAKDLLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDDIPKSDKQDVSDDNLVLSKSQNDYMEXXXXXXXXKQEKDDIPKSDKQDVSDDNLVLSKSQNDYMENYYLEDLDQKSLXXXXXXXXXXXNSIKPGKQLKVXXXXXXXXXXXXXXXXXXXKMINGVVKVKKEESSNFPKETNGKXXXXXXXXXXXSPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFF 1238
            D D DLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY                                       MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETF 
Sbjct: 1519 DADKDLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY---------------------------------------MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFL 1829

HSP 2 Score: 117.472 bits (293), Expect = 3.637e-146
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = -1

Query:   23 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 193
            FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV
Sbjct: 1828 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 1884

[Close]

BLAST of SMED30008638 vs. Planmine SMEST
Match: SMESG000013006.1 (SMESG000013006.1)

HSP 1 Score: 421.779 bits (1083), Expect = 3.855e-146
Identity = 308/350 (88.00%), Postives = 308/350 (88.00%), Query Frame = -3

Query:  189 DMDLDLINRCEACKDIILKAKERQEYEAAKNAKDLLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDDIPKSDKQDVSDDNLVLSKSQNDYMEXXXXXXXXKQEKDDIPKSDKQDVSDDNLVLSKSQNDYMENYYLEDLDQKSLXXXXXXXXXXXNSIKPGKQLKVXXXXXXXXXXXXXXXXXXXKMINGVVKVKKEESSNFPKETNGKXXXXXXXXXXXSPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFF 1238
            D D DLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY                                       MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETF 
Sbjct: 1517 DADKDLINRCEACKDIILKAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQEKDDIPKSDKQDVSDDNLVLSKSQNDY---------------------------------------MENYYLEDLDQKSLNNNENINPDNRNSIKPGKQLKVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNSSNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKLDIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGTSEAVHKAREFICDLVEGKITGNHVIQESETFL 1827

HSP 2 Score: 117.087 bits (292), Expect = 3.855e-146
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = -1

Query:   23 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 193
            FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV
Sbjct: 1826 FLTKSANNPKISTEFSNITNSSDSEYPKAIHPKTLSLVRHNGKLNKVGYSKFSLTPV 1882

The following BLAST results are available for this feature:

BLAST of SMED30008638 vs. Ensembl Human
BLAST of SMED30008638 vs. Ensembl Celegans
BLAST of SMED30008638 vs. Ensembl Fly
BLAST of SMED30008638 vs. Ensembl Zebrafish
BLAST of SMED30008638 vs. Ensembl Xenopus
BLAST of SMED30008638 vs. Ensembl Mouse
BLAST of SMED30008638 vs. UniProt/SwissProt
BLAST of SMED30008638 vs. TrEMBL
BLAST of SMED30008638 vs. Ensembl Cavefish
BLAST of SMED30008638 vs. Ensembl Sea Lamprey
BLAST of SMED30008638 vs. Ensembl Yeast
BLAST of SMED30008638 vs. Ensembl Nematostella
BLAST of SMED30008638 vs. Ensembl Medaka
BLAST of SMED30008638 vs. Planmine SMEST

BLAST of SMED30008638 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ANKRD17	2.062e-8	33.68	ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD17	2.795e-8	33.68	ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD17	2.819e-8	33.68	ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD17	2.856e-8	33.68	ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:H...	[more]
ANKHD1	1.186e-6	27.43	ankyrin repeat and KH domain containing 1 [Source:...	[more]

BLAST of SMED30008638 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pes-4	1.749e-7	27.69	Patterned Expression Site [Source:UniProtKB/TrEMB...	[more]

BLAST of SMED30008638 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mask	6.170e-7	40.58	gene:FBgn0043884 transcript:FBtr0334942	[more]
mask	6.221e-7	40.58	gene:FBgn0043884 transcript:FBtr0345261	[more]
mask	7.048e-7	40.58	gene:FBgn0043884 transcript:FBtr0305043	[more]
mask	7.608e-7	40.58	gene:FBgn0043884 transcript:FBtr0334943	[more]
mask	7.609e-7	40.58	gene:FBgn0043884 transcript:FBtr0084562	[more]

BLAST of SMED30008638 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ankhd1	2.012e-7	29.59	ankyrin repeat and KH domain containing 1 [Source:...	[more]
ankhd1	2.062e-7	29.59	ankyrin repeat and KH domain containing 1 [Source:...	[more]

BLAST of SMED30008638 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ANKRD17	5.400e-8	32.63	ankyrin repeat domain 17 [Source:NCBI gene;Acc:100...	[more]
ANKRD17	5.423e-8	32.63	ankyrin repeat domain 17 [Source:NCBI gene;Acc:100...	[more]
ANKRD17	5.618e-8	32.63	ankyrin repeat domain 17 [Source:NCBI gene;Acc:100...	[more]
ANKRD17	5.632e-8	32.63	ankyrin repeat domain 17 [Source:NCBI gene;Acc:100...	[more]
ANKRD17	5.876e-8	32.63	ankyrin repeat domain 17 [Source:NCBI gene;Acc:100...	[more]

BLAST of SMED30008638 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ankrd17	2.801e-8	33.68	ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd17	4.189e-8	33.68	ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd17	4.189e-8	33.68	ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd17	4.428e-8	33.68	ankyrin repeat domain 17 [Source:MGI Symbol;Acc:MG...	[more]
Ankhd1	2.710e-6	29.87	ankyrin repeat and KH domain containing 1 [Source:...	[more]

BLAST of SMED30008638 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|O75179\|ANR17_HUMAN	1.372e-7	33.68	Ankyrin repeat domain-containing protein 17 OS=Hom...	[more]
sp\|Q99NH0\|ANR17_MOUSE	2.930e-7	33.68	Ankyrin repeat domain-containing protein 17 OS=Mus...	[more]
sp\|Q9VCA8\|ANKHM_DROME	7.627e-6	40.58	Ankyrin repeat and KH domain-containing protein ma...	[more]
sp\|Q8IWZ3\|ANKH1_HUMAN	7.716e-6	27.43	Ankyrin repeat and KH domain-containing protein 1 ...	[more]

BLAST of SMED30008638 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A448WVQ0	2.260e-15	48.28	KH domain-containing protein (Fragment) OS=Protopo...	[more]
A0A0R3XB68	3.641e-15	48.84	KH domain-containing protein OS=Hydatigena taeniae...	[more]
A0A158R6V9	1.383e-14	38.06	ANK_REP_REGION domain-containing protein OS=Taenia...	[more]
A0A068Y3R2	2.363e-14	37.31	Ankyrin domain repeat containing protein OS=Echino...	[more]
W6UMR4	2.391e-14	37.31	Ankyrin repeat domain-containing protein OS=Echino...	[more]

BLAST of SMED30008638 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ankhd1	3.385e-7	29.31	ankyrin repeat and KH domain containing 1 [Source:...	[more]

BLAST of SMED30008638 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30008638 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30008638 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30008638 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ankhd1	1.172e-6	27.55	ankyrin repeat and KH domain-containing protein 1 ...	[more]
ankhd1	1.183e-6	27.55	ankyrin repeat and KH domain-containing protein 1 ...	[more]
ankhd1	1.205e-6	27.55	ankyrin repeat and KH domain-containing protein 1 ...	[more]

BLAST of SMED30008638 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000013006.1	7.018e-27	90.63	SMESG000013006.1	[more]
SMESG000013006.1	3.637e-146	88.00	SMESG000013006.1	[more]
SMESG000013006.1	3.855e-146	88.00	SMESG000013006.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30008638 ID=SMED30008638|Name=SMED30008638|organism=Schmidtea mediterranea sexual|type=transcript|length=1813bp
TGTTATTATTATTATTATTATTTACTGGAGTTAAACTGAATTTGCTATAG
CCAACTTTATTCAGCTTTCCATTGTGACGAACCAATGACAAAGTTTTAGG
ATGAATAGCTTTGGGATATTCTGAATCAGAGCTATTAGTAATATTACTGA
ACTCGGTGGAAATTTTCGGATTATTTGCAGATTTCGTCAAAAAAAGTTTC
AGATTCTTGAATTACATGATTTCCCGTTATTTTACCTTCAACTAAATCAC
AGATAAACTCTCTAGCCTTATGTACTGCTTCTGATGTTCCTTTTAAATGC
ACAGTTCTTTCATCTAGTTCATTTGCCATGCTGTCTATATCTATGTGAGC
TCCAGTTATTTGCCTAATAGCATTGACAACAGAACCCCCCTGTCCAATGA
CTTTACCAATATCCTTTTTATTCACTTGAATATCTAATTTCTTTTTACTT
CCTCCGATTTTCTGCCAATTACTACTAATTGATTCTTCAATTTTATTTGA
TTGGACATTTTTACTAGAATTAATGTGAGTCCATGTGTTTCCTTTATTGG
TAATTTTATTTGGTGAAATATCCATTATATTCGAAGAATTTGACATAATT
TTTCCATTGGTTTCCTTGGGAAAATTTGATGATTCTTCTTTTTTAACTTT
CACAACTCCATTGATCATTTTCTTGGATTTTATTTCTTCAGTAGAATTGG
AAGACTTATTTTTTTCTGATTCTATTTTTACTTTTAATTGCTTACCGGGT
TTAATTGAATTTCGATTATCTGGATTAATATTTTCATTATTATTTAATGA
TTTCTGGTCTAAATCCTCAAGATAATAATTCTCCATGTAATCATTTTGAC
TTTTCGACAAAACCAAGTTATCATCTGACACGTCTTGTTTGTCACTTTTC
GGAATATCGTCTTTTTCTTGCTTAGCCTTTTTCTTTGCTTTCTTTTTCTC
CATGTAATCATTTTGACTTTTCGACAAAACCAAGTTATCATCTGACACGT
CTTGTTTGTCACTTTTCGGAATATCGTCTTTTTCTTGCTTAGCCTTTTTC
TTTGCTTTCTTTTTCTCCCGTTTTTTGGCTTGCTTTACTTCTTTTTGTTC
TTTTGCTTTTCTCTCTTCTTCTACTTGTTTTAAAAGGTCCTTTGCATTTT
TTGCAGCTTCATATTCTTGACGCTCTTTGGCTTTTAGAATAATATCTTTA
CAAGCCTCACATCTATTAATTAGATCCAAATCCATATCATTATAATGTGC
TTCATCAAAAAAATCAGTGAAATCTCGACATGACTCACATTTATCAATAC
TTAAATTAGATGATATTTGTGATCGATCAATGAAATAACTTAATTCCATG
TTCGATATATTTTTACACACTGTTTTAAATGATGATAAATCTGAACTATA
TATTGATCTGTAGTTGTTTTCGACACGTTGATTCAGAATTAATCCAGGCA
TATATTTAGGCATTAACATATAGTTAAATGCCATGACAGATTAACATTAG
AAGAAAGTTTAGAGCATTTGAAATATATCCCGAAACGTATTAAATCCAAA
GATAAATAAAATTGAACGAAAATCATAACAGGATTTAGAATTAAATTTTT
ATCATATTGATTAGAATTGACATTAGCACGCCTCAATAAATGACAAATAA
ATATCTCATTGTGATAGGCTAGCAGGTTTGAGAGAAATACGGTAGACATA
CATGTCATTACTCAGTTGGAGAAGAGAGTAGTTCAAATATGTGTGCATTT
ATAGACTTATTATTGAACGTATATAAATAGTAACAACAGAGAATTTGACG
TTATGTAGTCAAT

protein sequence of SMED30008638-orf-1

>SMED30008638-orf-1 ID=SMED30008638-orf-1|Name=SMED30008638-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=438bp
MAFNYMLMPKYMPGLILNQRVENNYRSIYSSDLSSFKTVCKNISNMELSY
FIDRSQISSNLSIDKCESCRDFTDFFDEAHYNDMDLDLINRCEACKDIIL
KAKERQEYEAAKNAKDLLKQVEEERKAKEQKEVKQAKKREKKKAKKKAKQ
EKDDIPKSDKQDVSDDNLVLSKSQNDYMEKKKAKKKAKQEKDDIPKSDKQ
DVSDDNLVLSKSQNDYMENYYLEDLDQKSLNNNENINPDNRNSIKPGKQL
KVKIESEKNKSSNSTEEIKSKKMINGVVKVKKEESSNFPKETNGKIMSNS
SNIMDISPNKITNKGNTWTHINSSKNVQSNKIEESISSNWQKIGGSKKKL
DIQVNKKDIGKVIGQGGSVVNAIRQITGAHIDIDSMANELDERTVHLKGT
SEAVHKAREFICDLVEGKITGNHVIQESETFFDEICK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000099	neuron

Vocabulary: **INTERPRO**
Term	Definition
IPR004087	KH_dom
IPR004088	KH_dom_type_1

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0003723	RNA binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 101..154
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..178

Main menu

SMED30008638

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

blazegraph-runner