SMED30008633
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008633 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30008633 aligns in the following genomic locations:
Homology
BLAST of SMED30008633 vs. TrEMBL
Match: A0A430Q543 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0003404 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 3.666e-10 Identity = 76/284 (26.76%), Postives = 139/284 (48.94%), Query Frame = 3 Query: 63 ISTSNSLSYKWFYKY---KYVYHKPKSYPSFENDWEHKMGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGII-CEPEEP-RQYTDDLPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLSEI--------------NLNIEEVLDEVIEDRR-ETLMRPIYDQKYGWFDKEKQLKAQY 854 I +S+SL+ +W+ Y V PK+ ++ DWE++ E + V L+D+Q L E K +T +++ K+ Q+ +++I K+L LA + ++ T+++R K L + R A + L+ R+ LR LR N RI + I+ E +P R YTDD + + +++ L+ +L+ S+++F K + L + +L+ + NL ++++ EVIE+R+ TL +PI DQ + W+ KE + + QY Sbjct: 72 ICSSSSLN-RWYLSYLRGTRVLKPPKNPFVYDRDWENEPYKPKAKDEYLLPVKSLKDFQSLDERCKNMFTYSFVYKRQEFQENLNEILKELGFEPTDDSLAAKIVRMTLELRYKKWRLKEHPRCITLKLATKCLMNARQRTLRLLRATNLSEFHRITSALKIVHYEHVDPYRSYTDD-----------PMERIRRMTVLKANLISSQQEFYTEKNETLTNLLNDLTSLVDSNHSEMKKKEAQNL-MKQLFYEVIEERQLATLHKPIEDQ-FLWYLKEAKQREQY 341
BLAST of SMED30008633 vs. TrEMBL
Match: A0A095BXL3 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_01954 PE=4 SV=1) HSP 1 Score: 70.0922 bits (170), Expect = 1.976e-9 Identity = 77/286 (26.92%), Postives = 142/286 (49.65%), Query Frame = 3 Query: 63 ISTSNSLSYKWFYKY---KYVYHKPKSYPSFENDWEHKMGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGII-CEPEEP-RQYTDD--LPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEK--------ELETIRKNLSEI------NLNIEEVLDEVIEDRRET-LMRPIYDQKYGWFDKEKQLKAQY 854 I +S+SL+ +W+ Y V PK+ ++ DWE++ E + V L+D+Q L E K +T +++ K+ Q+ +++I K+L LA + ++ T+++R K L + R A + L+ R+ LR LR N RI + ++ E +P R YTDD + R + ++E + +++ L+ +L+ S+++F K + + N SE+ NL ++++ EVIE+R+ T L +PI Q + W+ KE + + QY Sbjct: 72 ICSSSSLN-RWYLSYLRGTRVLKPPKNPFVYDRDWENEPYKPKAKDEYLLPVKSLKDFQSLDERCKNMFTYSFVYKRQEFQENLNEILKELGFEPTDDSLAAKIVRMTLELRYKKWRLKEHPRCITLKLATKCLMNARQRTLRLLRATNLSEFHRITSALKVVHYEHVDPYRLYTDDPMVQRKNSFRNECYQERIRRITVLKANLISSQQEFYTEKNEILTNLLNDLTLLVDSNNSEMKKKEAQNL-MKQLFHEVIEERQLTSLYKPIKHQ-FLWYLKEAKQREQY 354
BLAST of SMED30008633 vs. TrEMBL
Match: G4VLA7 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_074210 PE=4 SV=1) HSP 1 Score: 64.6994 bits (156), Expect = 7.263e-8 Identity = 71/285 (24.91%), Postives = 142/285 (49.82%), Query Frame = 3 Query: 63 ISTSNSLSYKWFYKY---KYVYHKPKSYPSFENDWEHKMGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGII-CEPEEP-RQYTDD--LPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLS-------------EINLNIEEVLDEVIEDRR-ETLMRPIYDQKYGWFDKEKQLKAQY 854 I +S+SL+ +W+ +Y V + PK+ ++ DWE++ +E + + +D+Q L E K +T ++ K+ ++ ++++ +KL LA + ++ T+++R K L + R A + LI+ R+ LR LR N +RI + I+ E +P R TDD + R + ++E + K+ + L+ S+++F K K L + +L+ E ++++ +EVIE+R+ +L +PI D ++ W+ KE + + Q+ Sbjct: 27 ICSSSSLN-RWYLRYLRAPRVLNPPKNPFLYDKDWENEPYKPKAKNEYLLPLKCSKDFQSLDERCKNLFTYNFVYKRQEFEENVAEMLEKLGFQPTDDSLAAKIVRMTLELRYKKWRLKEHPRCITLKLATKCLIRARQRSLRLLRATNLSEFNRITSALKIVRYEHVDPYRLCTDDPIVQRKISFRNECYQRRIAKMTVFKAKLISSEQEFYIEKNKTLTNLLGDLTLLVDSNNPETKQKEAEHLMKQLFNEVIEERQLNSLRKPIED-RFLWYTKEGEKREQF 309
BLAST of SMED30008633 vs. TrEMBL
Match: A0A183QYE8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 9.734e-8 Identity = 71/285 (24.91%), Postives = 142/285 (49.82%), Query Frame = 3 Query: 63 ISTSNSLSYKWFYKY---KYVYHKPKSYPSFENDWEHKMGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGII-CEPEEP-RQYTDD--LPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLS-------------EINLNIEEVLDEVIEDRR-ETLMRPIYDQKYGWFDKEKQLKAQY 854 I +S+SL+ +W+ +Y V PK+ ++ DWE++ +E + + +D+Q L E K +T +++ K+ ++ ++++ +KL LA + ++ T+++R K L + R A + LI+ R+ LR LR N +RI + I+ E +P R TDD + R + ++E + K+ + L+ S+++F K K L + +L+ E ++E+ +EVI++R+ +L +PI D ++ W+ KE + + Q+ Sbjct: 27 ICSSSSLN-RWYLRYLRAPRVLTPPKNPFLYDKDWENEPYKPKAKNEYLLPLKCSKDFQSLDERCKNLFTYSFVYKRQEFEENVAEMLEKLGFQPTDDSLAAKIVRMTLELRYKKWRLKEHPRCITLKLATKCLIRARQRSLRLLRATNLSEFNRITSALKIVRYEHVDPYRLCTDDPIVQRKISFRNECYQRRITKMTVFKAKLISSEQEFYIEKNKTLTNLLGDLTLLVDSNNPETKQKEAEHLMKELFNEVIKERQLNSLRKPIED-RFLWYTKEGEKREQF 309
BLAST of SMED30008633 vs. TrEMBL
Match: A0A5J4NLW9 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0012013 PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 6.265e-7 Identity = 68/276 (24.64%), Postives = 114/276 (41.30%), Query Frame = 3 Query: 87 YKWFYKYKYVYHKPKSYPSFENDWEHKMGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGIICEPEEPRQYTDDLP-------RYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLSEINLN-------------IEEVLDEVIEDRRETLMRPIYDQKYGWFDKEKQLKAQY 854 YK F++ K V PK SF NDWEHK EP P+ ++ C + + Q + A HP I+ T++IR K+ L + R+K + R LI R LR LR N +R+ K + I P + DLP R + V+ + ++ K+ +L+ DL S++ F KEK+L + ++L+ + N ++ + EVI++R+ ++ W+ E + +Y Sbjct: 35 YKHFFQNKTV-ELPKEVNSFPNDWEHKP------YEPFS-----------PQTVRSC-----VQQSPSFQNWSTGYAVSF------HPSPIPVIRMTMRIRFKKRMLQEHPRNKTLRASTRALINARFRTLRLLRATNLPEFNRLTKALEITGF-RHPDPF--DLPETNPIAERKRAVRADCFQLRLTKLANLKVDLAASEQTFYERKEKQLNKVLQDLASLQRNESSDENMESAKRMLKSLFQEVIKERQNEVLSGPEQDALSWYANEAIQRERY 278
BLAST of SMED30008633 vs. Planmine SMEST
Match: SMESG000057402.1 (SMESG000057402.1) HSP 1 Score: 464.922 bits (1195), Expect = 4.538e-166 Identity = 232/234 (99.15%), Postives = 233/234 (99.57%), Query Frame = 3 Query: 168 MGFDAKLSEPIDGVTELEDYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGIICEPEEPRQYTDDLPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLSEINLNIEEVLDEVIEDRRETLMRPIYDQKYGWFDKEKQLKAQYEKKVL 869 MGFDAKLSEPIDGVTELE YQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGIICEPEEPRQYTDDLPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLSEINLNIEEVLDEVIEDRRETLM+PIYDQKYGWFDKEKQLKAQYEKKVL Sbjct: 1 MGFDAKLSEPIDGVTELEGYQKLPEILKKCYTNAYLDKKSLMQKEISDIAKKLQVNDFSHPLAFQFIKRTVQIRNGKKSLHDMYRDKLKYRAIRKLIQLRKADLRQLRIENGELVDRIVKQMGIICEPEEPRQYTDDLPRYKTVKDEAKLIKQKKVKSLREDLVQSKEKFLAMKEKELETIRKNLSEINLNIEEVLDEVIEDRRETLMQPIYDQKYGWFDKEKQLKAQYEKKVL 234 The following BLAST results are available for this feature:
BLAST of SMED30008633 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30008633 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30008633 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30008633 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30008633 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30008633 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008633 ID=SMED30008633|Name=SMED30008633|organism=Schmidtea mediterranea sexual|type=transcript|length=1153bpback to top protein sequence of SMED30008633-orf-1 >SMED30008633-orf-1 ID=SMED30008633-orf-1|Name=SMED30008633-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=301bp MFKTYLLFNESLIFVTNRFISTSNSLSYKWFYKYKYVYHKPKSYPSFENDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|