Guanylate cyclase domain-containing protein
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008588 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 362.459 bits (929), Expect = 3.218e-115 Identity = 228/620 (36.77%), Postives = 345/620 (55.65%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 348.977 bits (894), Expect = 7.033e-110 Identity = 224/642 (34.89%), Postives = 344/642 (53.58%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLE----------------------NLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+ NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I + ++F +I+ K + +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQKNESKHISDLHHCFLGIYLCCNNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQF------LIEEKESKEEDFYEDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 626
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 345.895 bits (886), Expect = 3.595e-109 Identity = 219/595 (36.81%), Postives = 332/595 (55.80%), Query Frame = 2
Query: 242 LEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
++ A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 6 IKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 584
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 345.895 bits (886), Expect = 1.429e-108 Identity = 221/601 (36.77%), Postives = 334/601 (55.57%), Query Frame = 2
Query: 224 EAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
+ W+ EA D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 53 KCWDNRKEA-QLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 636
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:4687]) HSP 1 Score: 325.479 bits (833), Expect = 6.440e-102 Identity = 204/551 (37.02%), Postives = 310/551 (56.26%), Query Frame = 2
Query: 374 FGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 2 FGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 536
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-35 (Soluble guanylate cyclase gcy-35 [Source:UniProtKB/Swiss-Prot;Acc:O02298]) HSP 1 Score: 397.897 bits (1021), Expect = 2.027e-128 Identity = 224/608 (36.84%), Postives = 361/608 (59.38%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNF---LPYHIIFDSMLYVRQSGYMLQK-LCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLL--FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL--EYVFEGRGEVEIKGKGLMKTYFL 1957
M G IH ++LV K G++ WE+I+ + + + + H Y D L+ ++A+ + + +E EA+G ++T G+ +++ + D+ FL++LD+LH YF V + + PSF D +++ ++LHY S R GLYP +KG++ +A+ + TE+ K+ + ++ +H+V +I ++IE + KSIS+ + + + +F PYHI FD L+V G ++K + ++ E ++ + L+ P VPF SI+ + NS F F L + + N+ +LL+G + F+D+ L++ S + T+ E+ E+NL LSD+ +D TR+++++NQ R++++E+++ L E T +L+K LE EK + + LLC+++P VA L +GK + A++F CT+LF+DIVTFT+I + C P DVV +LN LY FD+ + AYKVETIGDAYM+V GVPE HAE + ++ M+ S V SP++ K ++IR+G+H GPVVAGVVG KMPR+CLFGD VNVA++MES+G+ +IH S++ L YVF RG EI+GKG+M TYFL
Sbjct: 1 MFGWIHESFRQLVTRKYGKDIWEKIVHMSKFELGTESEIAHYYNDDETLRLVNSMANVIGIPIEEIWEAYGGFLIQFTMETGWDELLRAMAPDLEGFLDSLDSLH-YFIDHVVYKTKLRGPSFRCDVQADGTLLLHYYSKRSGLYPIVKGVVREVARRIYDTEVVMKV-QERKQEHLDAFVTEHVVFVI-TQIENANSTQP---KSISSKADSQIDLSTGIYEISSSDFSLAFPYHICFDPDLFVEHFGNFIKKTFPNAMRQETR----VTDLLELVHPEVPFSYESIKYYKNSLFVFRLKGLGDIVHNANDEAKTVLLKGSMVFIDEGKYILYMCSVNVTTVRELIERNLHLSDMQRHDGTRDVIMLNQSRMSQVELNRTLEETTKKLKKMAQELEIEKQKTDELLCELMPASVADSLRSGKAMDAKEFADCTLLFTDIVTFTNICAMCTPYDVVTLLNDLYLRFDRLVGLHDAYKVETIGDAYMIVGGVPERCENHAERVLNISIGMLMESKLVLSPITHKPIKIRLGVHCGPVVAGVVGIKMPRYCLFGDTVNVANKMESNGIQCKIHVSETGKLNGLKANPSYVFIDRGNTEIRGKGMMYTYFL 598
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-36 (Soluble guanylate cyclase gcy-36 [Source:UniProtKB/Swiss-Prot;Acc:Q6DNF4]) HSP 1 Score: 381.719 bits (979), Expect = 2.165e-122 Identity = 223/635 (35.12%), Postives = 350/635 (55.12%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE----SRFRQHMVIIIQSKIEAQYGSLAELDKSI---STNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAF------TFLLFRNYKNLTN-----------ETDSGIL---------LRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLL 1960
M G IH +++L++ GE W ++LE A + ++ ++ H Y D+ F+L++AV+ L +T ++ E +G +YT G+ +I ++ +++ FL+NLD+LH YF V + ++ PSF + + + LHY + R GLYP +KG+L AK FK ++ IT T + + R +H++ ++++ Q A L ++ S N++ + H ++ PYH++ D + Q G L KD L +I + P +P D SI FIN+ F T + F+ N E ++G L L+GQ+ + +++ SP + ++ E+ + L L+ +P++D TR+L+L+NQQRL+++E++ L QL+ LE EK + + LL +MLPP VAQQL G V+A +++ T++F+D+ TF I C P D+V +LN L++ FD+ AYKVET+GD+YM V G+P++ H E+I A+ MV + V P++ L IR GIHSGPVVAGVVG KMPR+CLFGD VN +SRMESH GRIHCS++ + + + FE RG V+IKGKG M TYFLL
Sbjct: 1 MFGFIHESIRQLMIRTYGEAFWSKVLERAGFEAGKENIINHYYSDADTFSLVDAVSVILKVTREQVWEMYGCFLIQYTMETGWDDLIRSMSPNLKGFLDNLDSLH-YFIDHVVYKANLRGPSFRCEDNPDGTITLHYYTGRPGLYPIVKGVLREAAKRVFKLDVSM-TITGRTQRSVQMATGERIEEHVIFLVKTLNTDQSNEEA-LGTAVVQHSNNYKIRLTHMDFIST------FPYHMVVDQDCKIVQVGRELYNHIP--KDLLSVGTPLMRIFEVTRPQIPLDFDSICNFINAVFVLQVKTTPMEFQRNANKRAAQAIEASENLYEDNNGALALSQSQHLKLKGQMMLMSSGGHIMYLCSPYVTSIPELLQYGLRLTAMPIHDPTRDLILLNQQRLSDVEMNLQLEANNEQLENMAKDLEVEKGKTDALLREMLPPSVAQQLKQGLSVEAREYEEATVMFTDVPTFQQIVPLCTPKDIVHLLNELFTKFDRLIGIQKAYKVETVGDSYMSVGGIPDLVDDHCEVICHLALGMVMEARTVCDPITNTPLHIRAGIHSGPVVAGVVGAKMPRYCLFGDTVNTSSRMESHSPIGRIHCSENAKKCAESTGRFEFEPRGRVQIKGKGEMNTYFLL 624
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-32 (Soluble guanylate cyclase gcy-32 [Source:UniProtKB/Swiss-Prot;Acc:Q6DNF7]) HSP 1 Score: 342.428 bits (877), Expect = 3.287e-107 Identity = 207/633 (32.70%), Postives = 350/633 (55.29%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEI----QFKIITSATDNNPESRFRQHMVIIIQSKIEAQY---GSLAELDKSISTNFRPKYPHKSL-VNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAF-----TFLLFRNY-----------------KNLTNETDSG--ILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFL 1957
M G IH +++LV+ GE+ W ++LE + ++ ++ ++ H Y D+ + L+++V+ L +T D+ E +G Y+ G+ +++ ++ +++ FL+NLD+LH YF V + ++ PSF ++ + ++LHY + R GLY +KG++ +AK F +I Q + S NN E R +H+V +I + E + GS L S + NF P +L ++ LPYH + D + Q G L P + Q +L +I + P +P D +I FIN+ F T L + + ++ TNE G + L+GQ+ L +++ SP + +++E+ + + L+ +PL+D TR+L+L+NQQRL+++E++ L QL+ T LE E+ + + +L MLP ++AQQLL+G+ ++A + + T++F D+ F QC+P D+V+MLN ++ D+ G YKVET+ D+YM V+G+P+ T HAE + A+ M+ + V PVS +R+GIHSG + AGVVG P++CLFG+ V +AS+MES GM G+I CS YQ + + F RG +++K +GL +TYFL
Sbjct: 1 MFGFIHESIRQLVIRNYGEDTWTQVLERSGFESGKENIMNHYYSDTDTYVLVDSVSLVLKVTKDQVWEMYGGFLITYSMEIGWDELVRSMSPNLKGFLDNLDSLH-YFIDHVVYKANLRGPSFRCEETPDGTLLLHYFTGRPGLYHIVKGVVKEVAKRVFDLDITLVVQGRTQRSVHMNNGE-RVEEHVVFLINNLSEPRRDSEGSEVSLLTSTNANF-PTIVDDTLGISLDDFSKALPYHFVIDESCKLVQCGSELHNHI-PNELLQPGTPIL-RIFEINRPQIPLDFENICNFINAVFVLQVKTSPLKKKHMDAMSQEELKQEMETLDEDATNELTQGHHLKLKGQMMLLASKKHIIYLCSPYVTSINELMQYGMRLTAMPLHDATRDLILLNQQRLSDVEVNLQLEANNEQLETMTRELELERQKTDSILKDMLPRRIAQQLLSGEHIEACEHE-ATVMFCDLPAFQQAIPQCSPKDIVNMLNEIFRKLDRIVVIRGVYKVETVSDSYMAVSGIPDYTPEHAENMCHVALGMMWEARSVIDPVSKTPFLLRIGIHSGTITAGVVGTVHPKYCLFGETVTLASQMESLGMAGKIQCSKWAYQKAMETGRFEFSPRGRIDVKQRGLTETYFL 627
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-34 (Soluble guanylate cyclase gcy-34 [Source:UniProtKB/Swiss-Prot;Acc:P92006]) HSP 1 Score: 339.347 bits (869), Expect = 4.260e-106 Identity = 203/636 (31.92%), Postives = 353/636 (55.50%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEI----QFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDK---SISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDE--QHDVILLSKIASLIVPAVPFDAVSIRRFINSAF-----TFLLFRNYKN------------------LTNETDSG--ILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLL 1960
M G IH +++LV+ K GE+ W ++LE + +N ++ +V H Y D+ + L+++V+ L +T D+ E +G Y+ G+ +++ ++ +++ FL+NLD+LH YF V + ++ PSF ++ + ++LHY + R GLY +KG++ +AK F +I Q + S NN E R +H++ +I++ E + S S+ +F V+ + LPYH + D + Q G +L + + +E Q +L +I + P +P D +I FIN+ F T L + + N +NE G + L+GQ+ L +++ SP + +++E+ + + L+ +PL+D TR+L+L+NQQRL+++E++ L QL+ T LE E+ + + +L MLP K+A+QLL+G+ ++ +++ T++F D+ F I C P ++V +LN ++ D+ G YKVET+ D+YM V+G+P+ TS HAE + A+ M+ + V PV+ +R+G+HSG ++AGVVG KMPR+CLFG+ V +AS+MES G+ G+I CS TY + + F RG + +KG+G ++TYFL+
Sbjct: 1 MFGFIHESIRQLVIRKYGEDVWLQVLERSGFENGKENIVNHYYSDTDTYVLVDSVSIVLKVTKDQIWEMYGGFLITYSMEIGWDELVRSMSPNLKGFLDNLDSLH-YFIDHVVYKANLRGPSFRCEENPDGTLMLHYFTGRPGLYHIVKGVVKEVAKLVFNLDISLVVQGRTQRSVHMNNGE-RVEEHVIFLIKNVEEPRRDSDTSTTSALTSVEPDFGEIIDDNLKVSLQDFSRALPYHFVLDESCRLVQCG---DELYNHIPNELLQPGTPIL-RIFEINRPQIPLDFENICNFINAVFVLQVKTSPLRKKHMNAMTKEEREQEVEAMEEEVESNELTQGCHLKLKGQMMMLSTKKHIIYLCSPYVTSINELMQFGMRLTAMPLHDATRDLILLNQQRLSDVEVNLQLEANNEQLETMTHELEVERQKTDSILKDMLPRKIAKQLLSGEHLEPCEYE-ATVMFCDLPAFQQIIPVCQPKNIVKLLNEVFFKLDRIVVLRGVYKVETVSDSYMTVSGIPDYTSEHAENMCHVALGMMWEARSVMDPVNKTPFLLRIGLHSGTIIAGVVGTKMPRYCLFGETVTLASQMESLGVAGKIQCSSWTYSKAMETGRFEFSPRGRINVKGRGDVETYFLM 629
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Match: gcy-37 (Soluble guanylate cyclase gcy-37 [Source:UniProtKB/Swiss-Prot;Acc:Q6DNF3]) HSP 1 Score: 300.056 bits (767), Expect = 5.369e-91 Identity = 174/619 (28.11%), Postives = 331/619 (53.47%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF--AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESR---FRQHMVIIIQSKIEAQYGS--LAELDKSISTNFRPKYPHKS--LVNARHMVNFLPYHIIFDSMLYVRQSG-YMLQKLCDPLKDEQHDVILLSKIASLIVPA-VPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSG--------ILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL---LALEYVFEGRGEVEIKGKGLMKTYFL 1957
M+G H+C+ L+L K G E E IL A F + Y D+ + A+ L L+ D+ E +G+ + G+ KM+ + +++ FL+NL+++H + + M P+F + + LHY S R+GL+P +KG++ A+ F+ +++ ++ + N E R +H++ ++ + G + + +T P + S LV R N PYH+ F+ + + G Y+L++ E + +S + L+ P+ + ++ ++N+ F F L + K NE G ++L+G++ ++D + +FI SP + T+ ++ L++SD+P++D TR+L+++NQ R+ +ME++K L E +++K T LE +K++ + LL + +PP +A+ L K V A++F C+++F+DI F +I+ C+P +++ ++ L+ FD+ E + YKV ++ D+Y++V GVP H E A+ ++ + +V P +S+++R+G+H GPVVAG+V ++ PRFC+ G+ VNV + SH PG++ S++ + L +VF G +E++ G + T+FL
Sbjct: 1 MIGWTHVCVSALILRKYGPEVLEEILRKAGYQEDIKFDIQCYYDDTETMRIFRVAATVLGLSVDDMWEMYGEFLITHACETGWQKMLFCMANNLQEFLDNLNSMHYFIDQIAFKSEMKGPTFQCEPFGESGLKLHYFSFRQGLFPIVKGLVRKTARTLFEMDVKVCML----ERNQERRKSGMVEHVIFSVEPDDNHRKGKRLFHKFRNTKTTENAPSFTLSSTILVGLRDFKNIFPYHVCFNKQMIIEHIGIYLLREY----GLENKKTLKVSDLMQLVQPSDIQLTYKNVLSYLNTLFIFQLKHHSKR--NEVQEGSSEAFQQPLVLKGEMMPINDGNSIIFICSPHVTTVRDILNLKLYISDMPMHDATRDLVMLNQSRICQMELNKKLEETMKKMKKMTEELEVKKSQTDRLLFEFVPPVIAEALRAAKTVPAQEFSDCSVIFTDIPDFFTISVNCSPTEIITVVTDLFHRFDRIIEKHKGYKVLSLMDSYLIVGGVPNANQYHCEDSLNLALGLLFEAKQVVVPKLERSVRLRIGVHCGPVVAGIVSQQKPRFCVLGNTVNVTKSICSHSSPGKVLVSNAVRTMVTKHLKSIFVFNANGYLELQS-GKVLTHFL 608
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0334926) HSP 1 Score: 344.739 bits (883), Expect = 2.262e-104 Identity = 206/622 (33.12%), Postives = 337/622 (54.18%), Query Frame = 2
Query: 191 MKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF---------RNYKNLTNETDS----GILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIPVE 2008
+ E + GEE WE I A D S F V YP+++ L + L ++ + ++ G F + ++GY +++ LG MR FL LD LHEY + M APSF + ++ + LHY S R+G + G + +A+ F+ E+ +++ E + + Q + + +LA L + P ++A + P+ ++F + + VR G L + L ++ ++ L+ P + F +I N+ F + +N L +E S + L+GQ+ ++++ +F+G+P + L+ + L+++D+ ++D +R+L+L Q+ E++++ + + +L+++ LL+ E R + LL QM+P +VA +L G+ E F +ILFSDIVTFT I S+ PM+VV MLNA+YS+FDK +E N YKVETIGDAYMVVAG P+ + HAE + + A+DMV+A ++ P +G+ L+IRVG+HSG VVAG+VG KMPR+CLFGD VN ASRMES + ++H S+ST + L+ Y RGE+++KGKG M TY+L R E ++P++
Sbjct: 9 LSEYIKSVYGEEKWEDIRRQAGID-SPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVR-------EEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLP-------ISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKK-----ITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISEST-KVLIGPNYKIIERGEIDVKGKGTMGTYWLEER-------ENRLPLQ 602
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0110961) HSP 1 Score: 344.354 bits (882), Expect = 3.366e-104 Identity = 206/622 (33.12%), Postives = 337/622 (54.18%), Query Frame = 2
Query: 191 MKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF---------RNYKNLTNETDS----GILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIPVE 2008
+ E + GEE WE I A D S F V YP+++ L + L ++ + ++ G F + ++GY +++ LG MR FL LD LHEY + M APSF + ++ + LHY S R+G + G + +A+ F+ E+ +++ E + + Q + + +LA L + P ++A + P+ ++F + + VR G L + L ++ ++ L+ P + F +I N+ F + +N L +E S + L+GQ+ ++++ +F+G+P + L+ + L+++D+ ++D +R+L+L Q+ E++++ + + +L+++ LL+ E R + LL QM+P +VA +L G+ E F +ILFSDIVTFT I S+ PM+VV MLNA+YS+FDK +E N YKVETIGDAYMVVAG P+ + HAE + + A+DMV+A ++ P +G+ L+IRVG+HSG VVAG+VG KMPR+CLFGD VN ASRMES + ++H S+ST + L+ Y RGE+++KGKG M TY+L R E ++P++
Sbjct: 9 LSEYIKSVYGEEKWEDIRRQAGID-SPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVR-------EEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLP-------ISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKK-----ITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISEST-KVLIGPNYKIIERGEIDVKGKGTMGTYWLEER-------ENRLPLQ 602
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gyc88E (gene:FBgn0038295 transcript:FBtr0306245) HSP 1 Score: 344.354 bits (882), Expect = 3.366e-104 Identity = 206/622 (33.12%), Postives = 337/622 (54.18%), Query Frame = 2
Query: 191 MKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF---------RNYKNLTNETDS----GILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIPVE 2008
+ E + GEE WE I A D S F V YP+++ L + L ++ + ++ G F + ++GY +++ LG MR FL LD LHEY + M APSF + ++ + LHY S R+G + G + +A+ F+ E+ +++ E + + Q + + +LA L + P ++A + P+ ++F + + VR G L + L ++ ++ L+ P + F +I N+ F + +N L +E S + L+GQ+ ++++ +F+G+P + L+ + L+++D+ ++D +R+L+L Q+ E++++ + + +L+++ LL+ E R + LL QM+P +VA +L G+ E F +ILFSDIVTFT I S+ PM+VV MLNA+YS+FDK +E N YKVETIGDAYMVVAG P+ + HAE + + A+DMV+A ++ P +G+ L+IRVG+HSG VVAG+VG KMPR+CLFGD VN ASRMES + ++H S+ST + L+ Y RGE+++KGKG M TY+L R E ++P++
Sbjct: 9 LSEYIKSVYGEEKWEDIRRQAGID-SPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEMHIELVR-------EEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLP-------ISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLGKK-----ITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLGTPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPDKDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHISEST-KVLIGPNYKIIERGEIDVKGKGTMGTYWLEER-------ENRLPLQ 602
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: Gycbeta100B (gene:FBgn0013973 transcript:FBtr0085753) HSP 1 Score: 234.187 bits (596), Expect = 5.661e-66 Identity = 134/394 (34.01%), Postives = 233/394 (59.14%), Query Frame = 2
Query: 815 LVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTS-IASQCNP---MDVVDMLNALYSLFDKASEDN---GAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL-LAL----EYVFEGRGEVEIKGKGL-MKTYFL 1957
L++ P+H++FD + + Q+G + ++ + +E +I ++ I P + + +I IN+ + L R + + L+GQ+ ++ + D LF P +M L ++ +K L++SD+PL+D R+L+L++++ AE +++K+L +T++LQ+T LE+EK + + LL +LP VA +L + + V +++ T++FS IV F A+ +P M +V MLN LY++FD ++ YKVET+GD YM V+G+P+ HA+ +A A+DM++ + V+ + +QI +GIHSG VV GV+G ++PR+CLFG+ VN+ SR E+ G+PGRI+ S+ TY+ L +A+ + E RG V +KGK M +FL
Sbjct: 327 LISPATFCKVFPFHLMFDRQMKIVQAGKAVSRVIPRVAEENCSLI---EVVEAIRPHLQLNFENILSHINTIYV-LQTRQGAMSSRHEQRFLRLKGQMMYIPETDRILFQCYPSVMNLDDLTKKGLYISDVPLHDAARDLVLLSEKFEAEYKLTKNLEMLTDKLQQTFRDLESEKQKTDRLLYSVLPKSVANELRHQRPVPPKRYDSVTLMFSGIVGFGQYCAANTDPDGAMKIVKMLNELYTVFDALTDSKRNLNVYKVETVGDKYMAVSGLPDHCEDHAKCMARVALDMMDMAKNVK--MGSNPVQITIGIHSGEVVTGVIGNRVPRYCLFGNTVNLTSRTETTGVPGRINVSEETYRLLCMAINQDDSFHLEYRGPVIMKGKPTPMDCWFL 714 HSP 2 Score: 122.094 bits (305), Expect = 2.913e-28 Identity = 63/166 (37.95%), Postives = 93/166 (56.02%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKII 664
M G ++ ++ LVL GEE WE+I + A FLV Y D I +NL+ A LN+ D+ LE FGK FF++ + GY K+++ LG R FL+NLDALH++ M APSF +K E++LHY S R GL + G++ +A + E++ I+
Sbjct: 1 MYGFVNYALELLVLKHFGEEIWEKIKKKAMVSMEGQFLVRQIYDDEITYNLIGAAVEILNIPADDILELFGKTFFEFCQDSGYDKILQVLGATPRDFLQNLDALHDHLGTLYPGMRAPSFRCTEKDG-ELLLHYYSERPGLEHIVIGIVKAVASKLHGVEVEIDIV 165
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Match: CG34357 (gene:FBgn0085386 transcript:FBtr0335239) HSP 1 Score: 218.394 bits (555), Expect = 4.286e-59 Identity = 113/234 (48.29%), Postives = 151/234 (64.53%), Query Frame = 2
Query: 1271 ISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVR-SPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLLGR 1966
+ K+ N + +++ T L+ E+ + LL +MLP VA++L G V E+F TI FSDIV FT+IA+ C+P+ VVD+LN LY++FD YKVETIGDAYMVV+G+P HAE IA A+D++ S + + G LQ+R+G+H+GP AGVVG MPR+CLFGD VN ASRMES G RIH S T L A Y E RG ++IKGKG+M T++LLG+
Sbjct: 1054 LEKYSNNLEELIRERTEQLDIERKKTEQLLNRMLPSSVAEKLKMGLAVDPEEFSDVTIYFSDIVGFTTIAAHCSPVQVVDLLNDLYTIFDATINAYNVYKVETIGDAYMVVSGLPVKIPDHAEQIATMALDLLHQSGRFNVKHLPGVPLQLRIGLHTGPCCAGVVGLTMPRYCLFGDTVNTASRMESTGSSWRIHMSQETRDRLDARGGYAIEPRGLIDIKGKGMMNTFWLLGK 1287
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 484.952 bits (1247), Expect = 5.144e-161 Identity = 259/649 (39.91%), Postives = 393/649 (60.55%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVI-IIQS-------------------------------KIEAQYGSLA---------ELDKSI--------STNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFE--GRGEVEIKGKGLMKTYFLL 1960
M G I+ C+K LV+++ GEE WE + A + F+ + Y D I L++ L+++++ L+ FG+ FF + K GY M+ LGG++ F+ENLDALH Y A++ ++M APSF +++ + ++LHY S RKGLY + G++ +AK+FF +E+ I+ + ++ + ++H+V ++Q +++A+Y +L EL +SI +F P YP + + N P+HI+FDS L V+ +G +QK L Q I L + +++ P V F+ SI++FINS F R + + LRGQ+ +++ +C +++ SP++ +L E+EE+ L L+DI +D TR+L+L+NQQRLAE+E+S L +L+ + LE EK + LL MLP VA QL GKRV+A +F++CTILFSD+VTFT+I + C P+ +V+MLNA+YS FD+ + + YKVETIGDAYMVV GVP T+ HAE +A A+ M A+ +V +P++G+ +QIRVG+H+GPV+AGVVGEKMPR+CLFGD VN ASRMESHG+P IH S T+ ++ + +FE RGE+E+KGKGLM+TYFLL
Sbjct: 1 MYGFINTCLKSLVIERFGEETWENLRLMAGVQET--FMTYEIYDDIITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEAMNAPSFRVERMDDGRILLHYYSDRKGLYHIVPGIIEAVAKDFFDSEVTMTILNQSEEDERTGK-KEHVVFHMVQKEKVTKRKAQTRQNNSEEEILEVKKDKEETMRRMKARYANLQLCPRKRSPWELVRSIVMLGQGNLRQSFTPSYPKTLWIEEQAFCNAFPFHIVFDSDLVVKHTGVNIQKFVPGL---QTAGIRLDEYFTIVHPEVIFNIQSIKKFINSQFVLKTRREMLPEVQQNQPTLKLRGQMMWMESLNCMIYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEIELSNQLERKKEELRILSRNLEIEKQKSEKLLYAMLPTHVANQLKEGKRVEAGEFKVCTILFSDVVTFTNICAACEPIQIVNMLNAMYSRFDRLTSIHNVYKVETIGDAYMVVGGVPVPTNTHAERVANFALGMRIAAREVTNPITGQPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHVSPFTFS-VIKDKGIFEMAERGEIEVKGKGLMRTYFLL 642
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b1 (guanylate cyclase 1 soluble subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-090313-160]) HSP 1 Score: 352.058 bits (902), Expect = 1.474e-111 Identity = 216/620 (34.84%), Postives = 342/620 (55.16%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFD---KASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ ++ LVL G + WE I A D FLV Y D+ ++L+ A + L + L+ FGK FF++ + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI+ K+I P+S H+ +I+ K + +LD F ++ ++ P+H++FD L + Q G + ++ L Q V LS + SL+ P + F I IN+ F LL N LT S + L+GQ+ L + + LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS + +V++LN +Y+ FD + ++ YKVET+GD YM V+G+PE + HA+ I A+DM+E + +V+ V +QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L ++E + E RG V +KGK MK +FL
Sbjct: 1 MYGFVNHALELLVLRNYGPDVWEDIKREAQVDVEGQFLVRIIYEDAKTYDLVAAASKVLKMDAGGILQMFGKMFFEFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVI------QPKSEECDHIKFLIEEKDSEEEAFYEDLD-----GFEENGTQETRISPYTFCKAFPFHLMFDKDLMLTQCGNAIFRVLPQL---QPGVCNLSSVFSLVRPHIDFSFHGILSHINTVFVLRSKEGLLNVETAENEDELTGTEISCLRLKGQMISLPETENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASAEGAIKIVNLLNDIYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQVK--VDEDPVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQSVENADPQFHLEYRGPVTMKGKKEPMKVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1b1 (guanylate cyclase 1 soluble subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-090313-160]) HSP 1 Score: 348.977 bits (894), Expect = 2.055e-110 Identity = 214/609 (35.14%), Postives = 336/609 (55.17%), Query Frame = 2
Query: 200 LVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFD---KASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
LVL G + WE I A D FLV Y D+ ++L+ A + L + L+ FGK FF++ + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI+ K+I P+S H+ +I+ K + +LD F ++ ++ P+H++FD L + Q G + ++ L Q V LS + SL+ P + F I IN+ F LL N LT S + L+GQ+ L + + LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS + +V++LN +Y+ FD + ++ YKVET+GD YM V+G+PE + HA+ I A+DM+E + +V+ V +QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L ++E + E RG V +KGK MK +FL
Sbjct: 6 LVLRNYGPDVWEDIKREAQVDVEGQFLVRIIYEDAKTYDLVAAASKVLKMDAGGILQMFGKMFFEFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVI------QPKSEECDHIKFLIEEKDSEEEAFYEDLD-----GFEENGTQETRISPYTFCKAFPFHLMFDKDLMLTQCGNAIFRVLPQL---QPGVCNLSSVFSLVRPHIDFSFHGILSHINTVFVLRSKEGLLNVETAENEDELTGTEISCLRLKGQMISLPETENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASAEGAIKIVNLLNDIYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQVK--VDEDPVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQSVENADPQFHLEYRGPVTMKGKKEPMKVWFL 598
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: GUCY1A2 (guanylate cyclase 1, soluble, alpha 2 [Source:NCBI gene;Acc:100330875]) HSP 1 Score: 252.292 bits (643), Expect = 1.148e-73 Identity = 182/587 (31.01%), Postives = 294/587 (50.09%), Query Frame = 2
Query: 374 FGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVT---QKSMIAPSFSI------DKKSNCE------MILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFK----IITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSIST--------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL---LALEYVFEGRGEV------EIKGKGLMKTYFL-LGRGVTHKN--GERKIPVENVS 2017
FG++FF +++ LGG+++ F DAL E+ + + S APSF ++++N E +ILH + + + G++ A+ +++E+ + + + E + L+ L + + T + P + P+H++ S + V Q G L+K K + H + S L+ P +P +I +++ FT + +L + + + L+GQ+ L +++ +F+GSPR+ L E+ + L LSDIP++D TR+++LV +Q A+ + K ++++ L+KT LE EK R LL + P VAQ+L G V+A+KF T+LFSDIV FT++ +QC PM V+ MLN LY+ FD YK+ETIGDAY V G+ HA+ IA A+ M+E S +V +P GK +++R+GIHSG V+AGVVG MPR+CLFG+ V +AS+ ES P I+ S +TYQ L + ++ R E+ EI G YFL G+ +H + R P+ VS
Sbjct: 42 FGEEFFGLCLEEN-ERVLRALGGNLQDFFNGFDALLEHIRTSCGRRASSEAPSFLCKDAPQENEETNAESGAGRTLILHCFNPDPTVGVAMPGLIRAAARRIYQSEVSVEEAPPTWLHTANEDGELSADSSPSSSPSPPSSSAPACLSFLIREVHTPPTSSSRSRQLSRSPLDLRIGLSTFCRAFPFHLVLGSNMEVLQVGEGLRKQA---KTDPHRTLTFSNSFELVSPRIPCSFQNILLRLSTPFTIRTRPDGSSL-DCREKVMELKGQMLHLPESNSIMFLGSPRVDRLEELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLEKTHQALEEEKRRTVDLLYSIFPGDVAQRLWQGLPVQAKKFDDVTMLFSDIVGFTAVCAQCTPMQVISMLNELYTRFDYQCGILDVYKIETIGDAYCVAGGLHRKIDSHAKPIALMALKMMELSEEVLTP-DGKPIKLRIGIHSGSVLAGVVGVMMPRYCLFGNNVTLASKFESGSHPRCINVSPTTYQLLRDDRSFTFIPRSRQELPDNFPKEIPG----ICYFLEAGKSQSHASLTSTRSAPIRKVS 618
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Match: gucy1a1 (guanylate cyclase 1 soluble subunit alpha 1 [Source:ZFIN;Acc:ZDB-GENE-050417-230]) HSP 1 Score: 233.417 bits (594), Expect = 2.214e-66 Identity = 131/345 (37.97%), Postives = 206/345 (59.71%), Query Frame = 2
Query: 845 LPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL 1879
P+HI+ D + + Q G L++ +D Q A ++ P + I +N+ F + ++ + + + L+GQ+ F+ + LF+GSP + L E+ + L+LSDIP+++ R+++LV +Q A+ + K L + L++ LE EK R LL + P VAQ+L G V+A+KF T+LFSDIV FT+I S+C PM VV+ML+ LY+ FD + YKVETIGDAY V G+ + + HA IA A+ M+E S++V +P+ G+ +++R+GIHSG V+AGVVG KMPR+CLFG+ V +A++ ES +P +I+ S +TY+ L
Sbjct: 288 FPFHILLDQEMGLLQIGDGLRRRLGRCRDGQRRPAFNEHFA-IVSPEIRASFQDILTMLNTQFLLRVKQHGASSADSPGKHMDLKGQMIFMSEMSALLFLGSPCVDKLEELTGRGLYLSDIPIHNALRDVVLVGEQTKAQDGLKKRLGKAKAALEQAHQALEEEKRRTVELLFTIFPGNVAQRLWQGLPVQAKKFDHVTVLFSDIVGFTAICSRCTPMQVVNMLSELYTRFDHHCGELDVYKVETIGDAYCVAGGLHKESPTHAVQIALMALKMMELSDEVTTPM-GEVIRMRIGIHSGSVLAGVVGVKMPRYCLFGNNVTLANKFESCSLPRKINVSPTTYRLL 630
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: ank1 (ankyrin 1 [Source:Xenbase;Acc:XB-GENE-5838516]) HSP 1 Score: 431.024 bits (1107), Expect = 8.296e-142 Identity = 240/612 (39.22%), Postives = 350/612 (57.19%), Query Frame = 2
Query: 404 RHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSK----------------------IEAQYGSLAELDKSIST------------------------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIPVENVSQCNAAVKCNAKEKSRTAEISNNHNDSPI 2101
+ GY M+ LGGD+ F+ NLDALH Y +++ + M APSF ++ + M LHY S R+GL + G++G +A +FFKTEI +II S +D + ++H+V ++ K E Y + +S FRP YP V+ LP+HI+FD L ++Q+G +QK+ L Q I L + S + P V ++R+FINS F F R + + LRGQ+ +++ +C L++ SP + TL E+EE+ + +SDI +DVTR+L+L+NQQRLAEME+S L +L+ + LE EK + +LL MLP VA QL GKRV+A +F+ CTILFSD+VTFT+I +QC P+ +V MLN++Y FD+ + + YKVETIGDAYMVV GVP S HAE +A A+ M+ + +V++PV+G +QIRVG+HSGPV+AGVVGEKMPR+CLFGD VN ASRMESHG+P +IH S + Y++L + RGE+++KGKG M+TYFL+ +N ++ A+ + + S+ N+ D+P+
Sbjct: 1 KSGYDHMLRTLGGDLYEFIANLDALHSYLSLSYQEMNAPSFRVEMNGDGSMHLHYYSDRRGLCHIVPGIMGAVAMDFFKTEISMEII-SQSDEEERTGKKEHIVFLVTQKPAFPPKCPTKPPLVSRGIKNKSFENPYNKYRDRKNQVSVCPVKKNHWAMIRGIVQLGKGRMLRGFRPVYPKDLCVDVETFCKGLPFHIVFDEELRIKQAGVSIQKIVPGL---QTTGIRLDQYFSTVHPEVTLTISNVRKFINSQFVFRTKREMMPESWRDRPMLELRGQMIWMEKLECMLYLCSPLLRTLHELEERQMHISDIAPHDVTRDLILLNQQRLAEMELSNQLERKKEELRILSKHLEEEKKKTELLLYAMLPKHVANQLKEGKRVEAGEFKECTILFSDVVTFTNICAQCEPIQIVFMLNSMYLRFDRLTTVHDVYKVETIGDAYMVVGGVPVPVSSHAERVANFALGMILMAKQVKNPVNGNPIQIRVGLHSGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPNKIHISPTVYRYLKGKNFEMTERGEIDVKGKGRMRTYFLIRNSSAKENEILGQAIKETDSGRASSQSLLDDTSKRNVQRNSPGDAPV 608
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 353.214 bits (905), Expect = 9.984e-112 Identity = 215/629 (34.18%), Postives = 344/629 (54.69%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGK-GLMKTYFLLGRGVTHKN 1984
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A LNL + L+ FG FF + + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I + ++F +I+ K + + D+ F +S ++ P+HI+FD L+V Q G + ++ L+ +++ + SL+ P + I IN+ F LL + LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN +Y+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + + RG V +KGK M+ +FL + +N
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQF------LIEEKDTREEDFYEDQDR-----FEENGTQESRISPYTFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLL---SVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSESEDELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQYRGPVSMKGKTDPMQVWFLSRKAAETEN 613
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 352.829 bits (904), Expect = 1.366e-111 Identity = 214/620 (34.52%), Postives = 341/620 (55.00%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGK-GLMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A LNL + L+ FG FF + + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I + ++F +I+ K + + D+ F +S ++ P+HI+FD L+V Q G + ++ L+ +++ + SL+ P + I IN+ F LL + LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN +Y+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + + RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQF------LIEEKDTREEDFYEDQDR-----FEENGTQESRISPYTFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLL---SVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSESEDELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQYRGPVSMKGKTDPMQVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 352.829 bits (904), Expect = 1.469e-111 Identity = 214/620 (34.52%), Postives = 341/620 (55.00%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGK-GLMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A LNL + L+ FG FF + + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I + ++F +I+ K + + D+ F +S ++ P+HI+FD L+V Q G + ++ L+ +++ + SL+ P + I IN+ F LL + LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN +Y+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + + RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQF------LIEEKDTREEDFYEDQDR-----FEENGTQESRISPYTFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLL---SVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSESEDELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQYRGPVSMKGKTDPMQVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Match: GUCY1B1 (guanylate cyclase 1 soluble subunit beta 1 [Source:NCBI gene;Acc:100379900]) HSP 1 Score: 341.273 bits (874), Expect = 5.198e-107 Identity = 214/638 (33.54%), Postives = 341/638 (53.45%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLE------------------NLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGK-GLMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A LNL + L+ FG FF + + GY ++ LG ++R FL+ NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I + ++F +I+ K + + D+ F +S ++ P+HI+FD L+V Q G + ++ L+ +++ + SL+ P + I IN+ F LL + LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN +Y+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + + RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVSAATKVLNLNAGDILQMFGNMFFVFCQESGYDTILRVLGSNVREFLQHFIYIIDKKACFLLFPINNLDALHDHLGTIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIVKTVAQQIHGTEIDMKVIQQRNEECDHTQF------LIEEKDTREEDFYEDQDR-----FEENGTQESRISPYTFCKAFPFHIMFDRDLFVTQCGNAIYRVLPQLQPGNCNLL---SVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSESEDELTGTEISCLRLKGQMIYLPEADNILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNTFCSKHASGEGAMKIVNLLNDIYTRFDILTDSRNNPYVYKVETVGDKYMTVSGIPEPCVHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLQYRGPVSMKGKTDPMQVWFL 622
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:MGI Symbol;Acc:MGI:2660873]) HSP 1 Score: 457.603 bits (1176), Expect = 9.010e-150 Identity = 245/636 (38.52%), Postives = 376/636 (59.12%), Query Frame = 2
Query: 164 SMLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE---SRFRQHMVIIIQSKIEAQYGSLAELDKSI----------------------------------STNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
+M G I+ C++ LV +K GEE WE++ +A + F+ + Y D+I L++ L+++ + L+ FG+ FFK+ K GY +M+ LGG++ F+ENLDALH Y A++ + M APSF ++ ++ M LHY S R+GL + G++ +AK+FF T++ I+ D N E + ++H+V ++ K Q L ++ F+P +P + + N P+H++FD L V+Q+G +QK + ++ L + S++ P V F+ SI +FINS F R ++ + LRGQ+ +++ C +F+ SP++ +L E+EE + LSDI +D TR+L+L+NQQRLAEME+S L + +L+ ++ L EK + LL MLP VA QL GK+V A +F+ CTILFSD+VTFT+I + C P+ +V+MLN++YS FD+ + + YKVETIGDAYMVV GVP HA+ +A A+ M ++ +V +PV+G+ +QIRVGIH+GPV+AGVVG+KMPR+CLFGD VN ASRMESHG+P ++H S + ++ L + RGE+E+KGKG M TYFL+
Sbjct: 81 TMYGFINTCLQSLVTEKFGEETWEKLKASAEVQDV--FMTYTVYDDTITVKLIQEACKALDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEGGADGAMRLHYYSDRRGLCHIVPGIIEAVAKDFFDTDVAMSIL----DMNEEVERTGKKEHVVFLVVQKARRQIRGAKSLQGTLLRKKERYLSIPVCPGEKSHSAVVRASVLLGKGPLGDTFQPVFPERLWIEEEVFCNAFPFHVVFDEALRVKQAGVNIQKYVPGILTQKFG---LDEYFSIVHPQVTFNISSICKFINSQFILKTRREMMPEAWKSQPTLKLRGQMIWMESLKCMVFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGKKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTNIHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAHRALENKGFEIVTRGEIEVKGKGKMTTYFLI 707
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:MGI Symbol;Acc:MGI:2660873]) HSP 1 Score: 454.521 bits (1168), Expect = 2.424e-148 Identity = 251/651 (38.56%), Postives = 383/651 (58.83%), Query Frame = 2
Query: 164 SMLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE---SRFRQHMVII----------------------IQSKIEAQYGSLAEL------------DKSIST---------------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
+M G I+ C++ LV +K GEE WE++ +A + F+ + Y D+I L++ L+++ + L+ FG+ FFK+ K GY +M+ LGG++ F+ENLDALH Y A++ + M APSF ++ ++ M LHY S R+GL + G++ +AK+FF T++ I+ D N E + ++H+V + IQ+K E+ G+L +KS S F+P +P + + N P+H++FD L V+Q+G +QK + ++ L + S++ P V F+ SI +FINS F R ++ + LRGQ+ +++ C +F+ SP++ +L E+EE + LSDI +D TR+L+L+NQQRLAEME+S L + +L+ ++ L EK + LL MLP VA QL GK+V A +F+ CTILFSD+VTFT+I + C P+ +V+MLN++YS FD+ + + YKVETIGDAYMVV GVP HA+ +A A+ M ++ +V +PV+G+ +QIRVGIH+GPV+AGVVG+KMPR+CLFGD VN ASRMESHG+P ++H S + ++ L + RGE+E+KGKG M TYFL+
Sbjct: 81 TMYGFINTCLQSLVTEKFGEETWEKLKASAEVQDV--FMTYTVYDDTITVKLIQEACKALDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEGGADGAMRLHYYSDRRGLCHIVPGIIEAVAKDFFDTDVAMSIL----DMNEEVERTGKKEHVVFLVVQKARRQIRGAKVNRPRDSENIQAKQESLQGTLLRKKERYLSIPVCPGEKSHSAVVRASVLLGKGPLGDTFQPVFPERLWIEEEVFCNAFPFHVVFDEALRVKQAGVNIQKYVPGILTQKFG---LDEYFSIVHPQVTFNISSICKFINSQFILKTRREMMPEAWKSQPTLKLRGQMIWMESLKCMVFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGKKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTNIHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAHRALENKGFEIVTRGEIEVKGKGKMTTYFLI 722
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b1 (guanylate cyclase 1, soluble, beta 1 [Source:MGI Symbol;Acc:MGI:1860604]) HSP 1 Score: 362.073 bits (928), Expect = 2.811e-115 Identity = 228/620 (36.77%), Postives = 345/620 (55.65%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1b1 (guanylate cyclase 1, soluble, beta 1 [Source:MGI Symbol;Acc:MGI:1860604]) HSP 1 Score: 352.058 bits (902), Expect = 1.487e-111 Identity = 217/588 (36.90%), Postives = 328/588 (55.78%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL 1879
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRKL 572
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Match: Gucy1a2 (guanylate cyclase 1, soluble, alpha 2 [Source:MGI Symbol;Acc:MGI:2660877]) HSP 1 Score: 253.832 bits (647), Expect = 2.178e-73 Identity = 177/535 (33.08%), Postives = 282/535 (52.71%), Query Frame = 2
Query: 314 NLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVT---QKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSAT----DNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFL----PYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAV--PFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL 1879
+L+ A+ L L E E FG++FFK +++ +G ++ F DAL E+ + Q ++ + SF + ++LHY + + GM+ K + ++ + I + +NP S + E + + T P+ + + R +N P+H++FDS + V Q G L+K L+ + H + ++ P + FD V +R +++ F NE D + ++GQ+ + ++ LF+GSP + L E+ + L LSDIP++D TR+++LV +Q A+ + K ++++ L++T LE EK + LL + P VAQQL G++V+A KF T+LFSDIV FT+I +QC PM V+ MLN LY+ FD YKVETIGDAY V +G+ + HA+ IA A+ M+E S +V +P GK++Q+R+GIHSG V+AGVVG +MPR+CLFG+ V +AS+ ES P RI+ S +TYQ L
Sbjct: 151 GILQCTANVLGLQFQEIQERFGEEFFKICFDEN-ERVLRAVGSTLQDFFNGFDALLEHIRTSFGKQATLESSSFLCKELPEGTLMLHYFHPHHTVGFAMLGMIKAAGKRIYHLNVEVEQIENEKLCFDGSNP-------------SNCSCLTFLIKECETTQITKDNPQGTSQVPADLRISINTFCRAFPFHLMFDSNMVVLQLGEGLRK---QLRYDTHRALKFEDCFEIVSPMINATFDRVLLR--LSTPFVIRTKPEASGTENE-DKVMEIKGQMIHVPESSAILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGLLDIYKVETIGDAYCVASGLHRKSLCHAKPIALMALKMMELSEEVLTP-DGKAIQMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLL 664
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|O75343|GCYB2_HUMAN (Guanylate cyclase soluble subunit beta-2 OS=Homo sapiens OX=9606 GN=GUCY1B2 PE=5 SV=2) HSP 1 Score: 417.542 bits (1072), Expect = 9.010e-136 Identity = 228/596 (38.26%), Postives = 343/596 (57.55%), Query Frame = 2
Query: 410 GYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE---SRFRQHMVIII-------------------------QSKIEAQYGSLAELDKSIST------------------------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKN---GERKIPVENVSQCNAA 2032
GY +M+ LGG++ F+ENLDALH Y A++ + M APSF +++ ++ +M LHY S R GL + G++ +AK+FF ++ I+ D N E + ++H+V +I Q ++A + + E ++S F P YP + + + N P+HI+FD L V+Q+ +QK L+ + I L + S+I P V F+ SIRRFINS F R + ++ + + L+GQ+ +++ C +++ SP++ +L E+EE N+ LSDI D TR+L+L+NQQRLAE+E+S L +LQ + L EK + LL MLP VA QL GK+V A +F+ CTILFSD+VTFT+I + C P+ +V++LN++YS FD+ + + YKVETIGDAYMVV GVP HA+ +A A+ M ++ +V +PV+G+ +Q+RVGIH+GPV+A VVG+KMPR+CLFGD VN ASRMESHG+P ++H S + Y+ L + RGE+E+KGKG M TYFL+ ++ G K PV++ A
Sbjct: 3 GYDRMLRTLGGNLMEFIENLDALHSYLALSYQEMNAPSFRVERGADGKMFLHYYSDRSGLCHIVPGIIEAVAKDFFDIDVIMDIL----DMNEEVERTGKKEHVVFLIVQKAHRKMRKTKPKRLQDSQGMERDQEALQAAFLKMKEKYLNVSACPVKKSHWDVVRSIVMFGKGHLMNTFEPIYPERLWIEEKTFCNAFPFHIVFDESLQVKQARVNIQKYVPGLQTQN---IQLDEYFSIIHPQVTFNIFSIRRFINSQFVLKTRREMMPVAWQSRTTLKLQGQMIWMESMWCMVYLCSPKLRSLQELEELNMHLSDIAPNDTTRDLILLNQQRLAEIELSNQLERKKEELQVLSKHLAIEKKKTETLLYAMLPKHVANQLREGKKVAAGEFKSCTILFSDVVTFTNICTACEPIQIVNVLNSMYSKFDRLTSVHAVYKVETIGDAYMVVGGVPVPIGNHAQRVANFALGMRISAKEVTNPVTGEPIQLRVGIHTGPVLADVVGDKMPRYCLFGDTVNTASRMESHGLPNKVHLSPTAYRALKNQGFKIIERGEIEVKGKGRMTTYFLIQNLNATEDEIMGRSKTPVDHKGSTQKA 591
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|P22717|GCYB2_RAT (Guanylate cyclase soluble subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Gucy1b2 PE=2 SV=1) HSP 1 Score: 418.698 bits (1075), Expect = 2.229e-135 Identity = 233/584 (39.90%), Postives = 347/584 (59.42%), Query Frame = 2
Query: 365 LEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE---SRFRQHMVIII----------------------QSKIEAQYGSLAEL------------DKSIST---------------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
L+ FG+ FFK+ K GY +M+ LGG++ F+ENLDALH Y A++ + M APSF +++ ++ M+LHY S R GL + G++ +AK+FF T++ I+ D N E + ++H+V ++ Q+ EA G+L + +KS ST F+P YP + V + P+HI+FD L V+Q+G +QK + ++ L + S+I P V F+ SI +FINS F + ++ + LRGQ+ +++ C +F+ SP + +L E+EE + LSDI +D TR+L+L+NQQRLAEME+S L + +L+ ++ L EK + LL MLP VA QL G++V A +F+ CTILFSD+VTFT+I + C P+ +V+MLN++YS FD+ + + YKVETIGDAYMVV GVP HA+ +A A+ M ++ +V +PV+G+ +QIRVGIH+GPV+AGVVG+KMPR+CLFGD VN ASRMESHG+P ++H S + ++ L + RGE+E+KGKG M TYFL+
Sbjct: 5 LKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSIL----DMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQK---FALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPNVRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLI 581
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|O02298|GCY35_CAEEL (Soluble guanylate cyclase gcy-35 OS=Caenorhabditis elegans OX=6239 GN=gcy-35 PE=1 SV=3) HSP 1 Score: 397.897 bits (1021), Expect = 2.578e-127 Identity = 224/608 (36.84%), Postives = 361/608 (59.38%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNF---LPYHIIFDSMLYVRQSGYMLQK-LCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLL--FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL--EYVFEGRGEVEIKGKGLMKTYFL 1957
M G IH ++LV K G++ WE+I+ + + + + H Y D L+ ++A+ + + +E EA+G ++T G+ +++ + D+ FL++LD+LH YF V + + PSF D +++ ++LHY S R GLYP +KG++ +A+ + TE+ K+ + ++ +H+V +I ++IE + KSIS+ + + + +F PYHI FD L+V G ++K + ++ E ++ + L+ P VPF SI+ + NS F F L + + N+ +LL+G + F+D+ L++ S + T+ E+ E+NL LSD+ +D TR+++++NQ R++++E+++ L E T +L+K LE EK + + LLC+++P VA L +GK + A++F CT+LF+DIVTFT+I + C P DVV +LN LY FD+ + AYKVETIGDAYM+V GVPE HAE + ++ M+ S V SP++ K ++IR+G+H GPVVAGVVG KMPR+CLFGD VNVA++MES+G+ +IH S++ L YVF RG EI+GKG+M TYFL
Sbjct: 1 MFGWIHESFRQLVTRKYGKDIWEKIVHMSKFELGTESEIAHYYNDDETLRLVNSMANVIGIPIEEIWEAYGGFLIQFTMETGWDELLRAMAPDLEGFLDSLDSLH-YFIDHVVYKTKLRGPSFRCDVQADGTLLLHYYSKRSGLYPIVKGVVREVARRIYDTEVVMKV-QERKQEHLDAFVTEHVVFVI-TQIENANSTQP---KSISSKADSQIDLSTGIYEISSSDFSLAFPYHICFDPDLFVEHFGNFIKKTFPNAMRQETR----VTDLLELVHPEVPFSYESIKYYKNSLFVFRLKGLGDIVHNANDEAKTVLLKGSMVFIDEGKYILYMCSVNVTTVRELIERNLHLSDMQRHDGTRDVIMLNQSRMSQVELNRTLEETTKKLKKMAQELEIEKQKTDELLCELMPASVADSLRSGKAMDAKEFADCTLLFTDIVTFTNICAMCTPYDVVTLLNDLYLRFDRLVGLHDAYKVETIGDAYMIVGGVPERCENHAERVLNISIGMLMESKLVLSPITHKPIKIRLGVHCGPVVAGVVGIKMPRYCLFGDTVNVANKMESNGIQCKIHVSETGKLNGLKANPSYVFIDRGNTEIRGKGMMYTYFL 598
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6DNF4|GCY36_CAEEL (Soluble guanylate cyclase gcy-36 OS=Caenorhabditis elegans OX=6239 GN=gcy-36 PE=1 SV=1) HSP 1 Score: 381.719 bits (979), Expect = 2.754e-121 Identity = 223/635 (35.12%), Postives = 350/635 (55.12%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYF---AVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPE----SRFRQHMVIIIQSKIEAQYGSLAELDKSI---STNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAF------TFLLFRNYKNLTN-----------ETDSGIL---------LRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLL 1960
M G IH +++L++ GE W ++LE A + ++ ++ H Y D+ F+L++AV+ L +T ++ E +G +YT G+ +I ++ +++ FL+NLD+LH YF V + ++ PSF + + + LHY + R GLYP +KG+L AK FK ++ IT T + + R +H++ ++++ Q A L ++ S N++ + H ++ PYH++ D + Q G L KD L +I + P +P D SI FIN+ F T + F+ N E ++G L L+GQ+ + +++ SP + ++ E+ + L L+ +P++D TR+L+L+NQQRL+++E++ L QL+ LE EK + + LL +MLPP VAQQL G V+A +++ T++F+D+ TF I C P D+V +LN L++ FD+ AYKVET+GD+YM V G+P++ H E+I A+ MV + V P++ L IR GIHSGPVVAGVVG KMPR+CLFGD VN +SRMESH GRIHCS++ + + + FE RG V+IKGKG M TYFLL
Sbjct: 1 MFGFIHESIRQLMIRTYGEAFWSKVLERAGFEAGKENIINHYYSDADTFSLVDAVSVILKVTREQVWEMYGCFLIQYTMETGWDDLIRSMSPNLKGFLDNLDSLH-YFIDHVVYKANLRGPSFRCEDNPDGTITLHYYTGRPGLYPIVKGVLREAAKRVFKLDVSM-TITGRTQRSVQMATGERIEEHVIFLVKTLNTDQSNEEA-LGTAVVQHSNNYKIRLTHMDFIST------FPYHMVVDQDCKIVQVGRELYNHIP--KDLLSVGTPLMRIFEVTRPQIPLDFDSICNFINAVFVLQVKTTPMEFQRNANKRAAQAIEASENLYEDNNGALALSQSQHLKLKGQMMLMSSGGHIMYLCSPYVTSIPELLQYGLRLTAMPIHDPTRDLILLNQQRLSDVEMNLQLEANNEQLENMAKDLEVEKGKTDALLREMLPPSVAQQLKQGLSVEAREYEEATVMFTDVPTFQQIVPLCTPKDIVHLLNELFTKFDRLIGIQKAYKVETVGDSYMSVGGIPDLVDDHCEVICHLALGMVMEARTVCDPITNTPLHIRAGIHSGPVVAGVVGAKMPRYCLFGDTVNTSSRMESHSPIGRIHCSENAKKCAESTGRFEFEPRGRVQIKGKGEMNTYFLL 624
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q02153|GCYB1_HUMAN (Guanylate cyclase soluble subunit beta-1 OS=Homo sapiens OX=9606 GN=GUCY1B1 PE=1 SV=1) HSP 1 Score: 362.459 bits (929), Expect = 1.545e-114 Identity = 228/620 (36.77%), Postives = 345/620 (55.65%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFS-IDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFDKASEDNG---AYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ ++ LV+ G E WE I + A D FLV Y DS ++L+ A + LNL E L+ FGK FF + + GY ++ LG ++R FL+NLDALH++ A M APSF D + +ILHY S R+GL + G++ +A++ TEI K+I N E Q ++ +SK E Y +LD+ F +S ++ P+HIIFD L V Q G + ++ L + + LLS + SL+ P + I IN+ F LL LT S + L+GQ+ +L + D LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS M +V++LN LY+ FD ++ YKVET+GD YM V+G+PE HA I A+DM+E + +V+ V G+S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L++ E + E RG V +KGK M+ +FL
Sbjct: 1 MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQ---RNEECDHTQFLIEEKESKEEDFY---EDLDR-----FEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQL--QPGNCSLLS-VFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQ--VDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFL 604
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1I8IZI1 (Guanylate cyclase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 630.558 bits (1625), Expect = 0.000e+0 Identity = 312/565 (55.22%), Postives = 403/565 (71.33%), Query Frame = 2
Query: 368 EAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV---------------------------KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLA----LEYVFEGRGEVEIKGKGLMKTYFLLGRG 1969
E FG FFKY K+HGY KMI LG D++ F++NLD+LH+Y + KSM+ PSFS + + + E ILHY S+R GL WIKGML +AKE F TEIQ K++ TD PESR++ H+ I+I + S + L S+ +P YP KSL+ + V + PYHIIFDS+L+++QSG +Q +C L+ + V+ L+++ L+ P V F + I+RF+NS+FT L R+ ++ + T+S +L++GQ+ F++D + FLF+ SPR+MTLSEM EKN+F+SD+PLYDVTRELL VNQQRLAE+EISKH++EMTN+++K T+ LE+EK + + LL QMLP KVA+QLL GKRV AEK+++CTILFSDIVTFT IASQC PM++VDMLN LYSLFDKA+E N YKVETIGDAYMVV GVPE T +HA ++EQAMDM+EAS +V SPV+ LQIRVG+H+GP VAGVVG+KMPRFCLFGD VNVASRMESHG+P RIH S +TY+ L Y FEGRGE+ IKGKGLM TYFLLG
Sbjct: 3 EMFGSHFFKYFKKHGYDKMIYTLGQDLKKFIQNLDSLHDYLQIDNKSMVPPSFSCNYEDSGEFILHYYSARAGLESWIKGMLTTVAKEIFSTEIQLKVMDIDTDFPPESRYQTHVRILIS------FASHSSLAASLDDFVQPCYPSKSLITVKSFVRYFPYHIIFDSLLFIKQSGAAIQSVCQTLRFKD-SVVKLTEVCELVNPPVNFSSHYIKRFLNSSFTLRLKRSIED--DCTESSLLIKGQMIFMEDTNLFLFVCSPRVMTLSEMMEKNIFMSDLPLYDVTRELLQVNQQRLAEIEISKHMDEMTNEMKKMTAELEDEKKKTHTLLSQMLPSKVAKQLLEGKRVAAGERTRDIPSVSPFCWTAASGGAILTSAEKYEICTILFSDIVTFTDIASQCTPMNIVDMLNNLYSLFDKATEVNKVYKVETIGDAYMVVGGVPERTDQHAHFVSEQAMDMIEASCRVASPVTNLPLQIRVGMHTGPTVAGVVGDKMPRFCLFGDTVNVASRMESHGVPSRIHVSPTTYKVLTEKCADKGYKFEGRGEINIKGKGLMSTYFLLGHA 558
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1I8IZT5 (Guanylate cyclase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 622.854 bits (1605), Expect = 0.000e+0 Identity = 312/579 (53.89%), Postives = 402/579 (69.43%), Query Frame = 2
Query: 368 EAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNET----------------DSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV---------------------------KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLA----LEYVFEGRGEVEIKGKGLMKTYFLLG 1963
E FG FFKY K+HGY KMI LG D++ F++NLD+LH+Y + KSM+ PSFS + + + E ILHY S+R GL WIKGML +AKE F TEIQ K++ TD PESR++ H+ I+I + S + L S+ +P YP KSL+ + V + PYHIIFDS+L+++QSG +Q +C L+ + V+ L+++ L+ P V F + I+RF+NS+FT L R+ ++ E+ +S +L+ GQ+ F++D + FLF+ SPR+MTLSEM EKN+F+SD+PLYDVTRELL VNQQRLAE+EISKH++EMTN+++K T+ LE+EK + + LL QMLP KVA+QLL GKRV AEK+++CTILFSDIVTFT IASQC PM++VDMLN LYSLFDKA+E N YKVETIGDAYMVV GVPE T +HA ++EQAMDM+EAS +V SPV+ LQIRVG+H+GP VAGVVG+KMPRFCLFGD VNVASRMESHG+P RIH S +TY+ L Y FEGRGE+ IKGKGLM TYFLLG
Sbjct: 3 EMFGSHFFKYFKKHGYDKMIYTLGQDLKKFIQNLDSLHDYLQIDNKSMVPPSFSCNYEDSGEFILHYYSARAGLESWIKGMLTTVAKEIFSTEIQLKVMDIDTDFPPESRYQTHVRILIS------FASHSSLAASLDDFVQPCYPSKSLITVKSFVRYFPYHIIFDSLLFIKQSGAAIQSVCQTLRFKD-SVVKLTEVCELVNPPVNFSSHYIKRFLNSSFTLRLKRSIEDDCTESSLLIKGKGWGGRCVSRESSLLINGQMIFMEDTNLFLFVCSPRVMTLSEMMEKNIFMSDLPLYDVTRELLQVNQQRLAEIEISKHMDEMTNEMKKMTAELEDEKKKTHTLLSQMLPSKVAKQLLEGKRVAAGERTRDIPSVSPFCWTAASGGAILTSAEKYEICTILFSDIVTFTDIASQCTPMNIVDMLNNLYSLFDKATEVNKVYKVETIGDAYMVVGGVPERTDQHAHFVSEQAMDMIEASCRVASPVTNLPLQIRVGMHTGPTVAGVVGDKMPRFCLFGDTVNVASRMESHGVPSRIHVSPTTYKVLTEKCADKGYKFEGRGEINIKGKGLMSTYFLLG 574
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1I8GHI0 (Guanylate cyclase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 550.821 bits (1418), Expect = 0.000e+0 Identity = 277/509 (54.42%), Postives = 351/509 (68.96%), Query Frame = 2
Query: 347 LTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQ 1873
++ DE E FG+ FFKY KRHGY KMI LG D++ F+ NLD+LH+Y + K M+ PSFS+ + + ++ + + P GML +AKE F TEIQ K++ TD ESR+R H ++I + S L S+ F+P P S + R + PYHI+FD L VRQSG +Q +C L+ V L+ + L+ P V F A IRRF+NSAFT LL R + + ET +LL+GQ+ +DD FLF SPR++TL EM EK L +SD+PLYDVTRELL VNQQRLAE+ IS+ L+EMT Q+++ T+ LE EK + LL QMLP KVA+QLL+GK+VKAEK+ +CTILFSDIVTFT IASQC PMDVVDMLN+LYSLFD A+E N YKVETIGDAYMVV GVPE T HAE++ EQAMDM+EA+ KV SPV+ K LQ+R+GIH+GP VAGVVG+KMPRFCLFGD VNVASRMESHG+P R+H S ++
Sbjct: 1 MSKDELYEMFGRYFFKYFKRHGYDKMIYTLGQDLKKFIRNLDSLHDYLQIDNKGMVPPSFSVIDMGD---VANFQALGRSPDPDPSGMLSTVAKEIFGTEIQLKVLNIETDFPAESRYRTHAKLLIT------FSSTGSLAVSLEDYFQPWCPAHSHITVRSFIRHFPYHIVFDEQLLVRQSGVGIQTVCHALRSPSEPV-RLTDVCDLVNPPVAFTASFIRRFLNSAFTLLLRRGPEETSPET--SLLLKGQMIHMDDTQLFLFFCSPRVLTLCEMMEKRLCMSDLPLYDVTRELLQVNQQRLAEIGISRQLDEMTYQMRRMTAELEVEKQKTYTLLSQMLPAKVAKQLLDGKKVKAEKYDICTILFSDIVTFTDIASQCTPMDVVDMLNSLYSLFDHATEVNRVYKVETIGDAYMVVGGVPERTVLHAELVCEQAMDMLEAAGKVCSPVTKKPLQVRIGIHTGPTVAGVVGDKMPRFCLFGDTVNVASRMESHGLPNRVHVSPEAHE 497
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1I8H552 (Guanylate cyclase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 534.258 bits (1375), Expect = 1.042e-180 Identity = 271/493 (54.97%), Postives = 341/493 (69.17%), Query Frame = 2
Query: 347 LTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRME 1825
++ DE E FG+ FFKY KRHGY KMI LG D++ F+ NLD+LH+Y + K M+ PSFS+ + + + + + P GML +AKE F TEIQ K++ TD ESR+R H ++I + S L S+ F+P P S + R + PYHI+FD L VRQSG +Q +C L+ V L+ + L+ P V F A IRRF+NSAFT LL R + + ET +LL+GQ+ +DD FLF SPR++TLSEM EK L +SD+PLYDVTRELL VNQQRLAE+ IS+ L+EMT Q+++ T+ LE EK + LL QMLP KVA+QLL+GK+VKAEK+ +CTILFSDIVTFT IASQC PMDVVDMLN+LYSLFD A+E N YKVETIGDAYMVV GVPE T +HAE++ EQAMDM+EA+ KV SPV+ K LQ+R+GIH+GP VAGVVG+KMPRFCLFGD VNVASRME
Sbjct: 1 MSKDELYEMFGRYFFKYFKRHGYDKMIYTLGQDLKKFIRNLDSLHDYLQIDNKGMVPPSFSVIDMGD---VANSQALGRSPDPDPSGMLSTVAKEIFGTEIQLKVLNIETDFPAESRYRTHAKLLIT------FSSTGSLAVSLEDYFQPWCPAHSHITVRSFIRHFPYHIVFDEQLLVRQSGVGIQTVCHALRSPSEPV-RLTDVCDLVNPPVAFTASFIRRFLNSAFTLLLRRGPEETSPET--SLLLKGQMIHMDDTQLFLFFCSPRVLTLSEMMEKRLCMSDLPLYDVTRELLQVNQQRLAEIGISRQLDEMTYQMRRMTAELEVEKQKTYTLLSQMLPAKVAKQLLDGKKVKAEKYDICTILFSDIVTFTDIASQCTPMDVVDMLNSLYSLFDHATEVNRVYKVETIGDAYMVVGGVPERTVQHAELVCEQAMDMLEAAGKVCSPVTKKPLQVRIGIHTGPTVAGVVGDKMPRFCLFGDTVNVASRME 481
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Match: A0A1S3IRP6 (guanylate cyclase soluble subunit beta-2 OS=Lingula unguis OX=7574 GN=LOC106166546 PE=3 SV=1) HSP 1 Score: 540.806 bits (1392), Expect = 1.826e-180 Identity = 303/693 (43.72%), Postives = 432/693 (62.34%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCE-MILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYG--------SLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPL---KDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLL-------FRNYKNLTNETDSGILLRGQICFLDDN-DCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIPV--------------ENVSQCNAAVKCNAKEKSRTAEISNNHNDSPIKYSQAHKSALCRIV 2143
M G IH+C++ LV+ K GEE WE ILE A CD++ DF+VFH Y D L+ AV+ L++ + LE FG+ FF+Y +GY KM+ LG D+ +F++NLD+LH A++ K++ APSF + S + +ILHY S RKGLYP + G++ + K F ++ ++I+ DN E + H+V ++ +A+ +L + +KS+ + F PK+P + ++ LPYH++FD + + QSG +QK+C L + HD L+ P +P + +I RFIN+ F + N K + ++GQ+ LD + +FI SPR+ +L+E+ E+N+FLSDIPLYDVTREL+L+NQQR+AE+EISK L+E T +L++T + L EK + + LL QMLP KVA QL G+ V+AEKF TILFSDIVTFT+IA+ C P+ +V+MLN LYS FD + +G YKVETIGDAYMVVAGVP+ HAE IA A+DMVEA+ +V SP +G+ LQIRVG+H G VVAGVVG+KMPR+CLFGD VN ASRMESHG+PG+IH S S + L Y+FE RG +EIKGKG M T+FL+GR NGE ++ + N+++ NA++K + +S++ ND S+ ++S C I+
Sbjct: 1 MYGQIHVCVRSLVIKKFGEEVWETILEKAQCDDTTDFMVFHRYTDQKTIGLVVAVSQVLDVPLEAVLELFGEYFFEYCLDNGYDKMLRTLGEDLPSFIQNLDSLHSLLAMSYKNIEAPSFRCEANSGEDTLILHYYSRRKGLYPIVIGLVRAVGKHLFNSDCTLEVISKTDDNPMEENSQDHVVFRVKVTDQAEKEDESKCRELALTQKEKSLRS-FVPKFPRRIHIDPSSFCKALPYHVVFDGTMTILQSGVQMQKICPDLMKPGGKLHDYF------ELVHPRMPLTSENIIRFINANFILQIKESMCGEVTNGKAVVPLRSKAFPIKGQMILLDQSVGHIMFICSPRLTSLTELMERNIFLSDIPLYDVTRELILLNQQRIAEIEISKKLDETTAELKRTATALNREKQKTDSLLYQMLPRKVADQLREGRTVEAEKFSEVTILFSDIVTFTNIAAACAPISIVEMLNELYSRFDALTSKHGVYKVETIGDAYMVVAGVPDSVPDHAERIANMALDMVEAAGEVISPATGRPLQIRVGMHLGGVVAGVVGKKMPRYCLFGDTVNTASRMESHGVPGKIHVSPSAREKLDNKPYIFEERGLIEIKGKGEMVTFFLIGR-----NGEVRLELMKDNNTTVRGGTLDTNLAEPNASLKWVDSHR-----MSSDQNDR--TGSRQNESRTCTII 674
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 487.649 bits (1254), Expect = 1.536e-162 Identity = 248/610 (40.66%), Postives = 375/610 (61.48%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKS------------ISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
M G I+ C++ LV++K GEE WE++ A ++ F+ + Y D I L++ L+++++ L+ FG+ FF + K GY M+ LGG++ F+ENLDALH Y A++ + M APSF +++ ++ +LHY S RKGL+ + G++ +AK+FF +++ I+ + + R+H++ ++ E G A L+ I +F P+YP + + + N P+HI+FD L V+ +G +QK L Q L + S++ P V + SI++FINS F R + + + + LRGQ+ +++ C +++ SP++ +L E+EE+ L L+DI +D TR+L+L+NQQRLAE+E+S L +L+ + LE EK + LL MLP VA QL GK+V A +F++CTILFSD+VTFT+I + C P+ +V MLN++YS FD+ + + YKVETIGDAYMVV GVP HAE +A A+ M A+ +V +P++GK +QIRVG+H+GPV+AGVVGEKMPR+CLFGD VN ASRMESHG+P IH S TY L + RGE+++KGKGLM TYFL+
Sbjct: 1 MYGFINTCLRTLVVEKFGEETWEKLRLLAEVQDT--FMTYEIYDDVITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEEMNAPSFRVERTNDGRTLLHYYSDRKGLHYIVPGIIEAVAKDFFGSKVTMTILEQPKEQERTGK-REHVIFLMTQTSEGAKGEEAHLNGGDGTVERKVMSDHIQHSFCPRYPKRLWIEEQAFCNAFPFHIVFDQNLVVKHTGVNIQKFVPGL---QKTGCRLDEYFSVVHPEVTLNIHSIKKFINSQFVLKTRREMLPESLQNQTMLKLRGQMVWMESLHCMVYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEIELSNQLERKKEELRILSRNLEVEKKKTETLLYAMLPRHVANQLKEGKKVDAGEFKVCTILFSDVVTFTNICAACEPIQIVHMLNSMYSRFDRLTNVHDVYKVETIGDAYMVVGGVPIPKDTHAERVANFALGMRIAAREVTNPITGKPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHLSPFTYSALKDKMFEIRERGEIQVKGKGLMDTYFLI 604
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 486.878 bits (1252), Expect = 3.476e-162 Identity = 248/611 (40.59%), Postives = 375/611 (61.37%), Query Frame = 2
Query: 164 SMLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKS------------ISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
S G I+ C++ LV++K GEE WE++ A ++ F+ + Y D I L++ L+++++ L+ FG+ FF + K GY M+ LGG++ F+ENLDALH Y A++ + M APSF +++ ++ +LHY S RKGL+ + G++ +AK+FF +++ I+ + + R+H++ ++ E G A L+ I +F P+YP + + + N P+HI+FD L V+ +G +QK L Q L + S++ P V + SI++FINS F R + + + + LRGQ+ +++ C +++ SP++ +L E+EE+ L L+DI +D TR+L+L+NQQRLAE+E+S L +L+ + LE EK + LL MLP VA QL GK+V A +F++CTILFSD+VTFT+I + C P+ +V MLN++YS FD+ + + YKVETIGDAYMVV GVP HAE +A A+ M A+ +V +P++GK +QIRVG+H+GPV+AGVVGEKMPR+CLFGD VN ASRMESHG+P IH S TY L + RGE+++KGKGLM TYFL+
Sbjct: 6 SQYGFINTCLRTLVVEKFGEETWEKLRLLAEVQDT--FMTYEIYDDVITLRLVQEACKMLDVSSEVVLKLFGEYFFSFCKMSGYDTMLRTLGGNLVEFIENLDALHSYLALSYEEMNAPSFRVERTNDGRTLLHYYSDRKGLHYIVPGIIEAVAKDFFGSKVTMTILEQPKEQERTGK-REHVIFLMTQTSEGAKGEEAHLNGGDGTVERKVMSDHIQHSFCPRYPKRLWIEEQAFCNAFPFHIVFDQNLVVKHTGVNIQKFVPGL---QKTGCRLDEYFSVVHPEVTLNIHSIKKFINSQFVLKTRREMLPESLQNQTMLKLRGQMVWMESLHCMVYLCSPKLRSLQELEERGLHLADIAQHDTTRDLILLNQQRLAEIELSNQLERKKEELRILSRNLEVEKKKTETLLYAMLPRHVANQLKEGKKVDAGEFKVCTILFSDVVTFTNICAACEPIQIVHMLNSMYSRFDRLTNVHDVYKVETIGDAYMVVGGVPIPKDTHAERVANFALGMRIAAREVTNPITGKPIQIRVGLHTGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGVPDHIHLSPFTYSALKDKMFEIRERGEIQVKGKGLMDTYFLI 610
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000013658.2 (pep primary_assembly:Astyanax_mexicanus-2.0:17:5336467:5343990:1 gene:ENSAMXG00000013166.2 transcript:ENSAMXT00000013658.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 441.039 bits (1133), Expect = 2.529e-145 Identity = 237/565 (41.95%), Postives = 341/565 (60.35%), Query Frame = 2
Query: 365 LEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSIST-------------------------------NFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNET--DSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLL 1960
L+ FG+ FF++ KR GY M+ LG ++ F+ENLDALH Y +++ K M APSF ++K S+ M+LHY S R+GL + G++G +AK+FF + I+ KI+ + + ++H+V +I + S + S T F P YP++ ++ + N P+HI+FD L VRQ+G +QK+ L Q I L + +++ P V F SIR+FINS F + + + + E+ D+ +L + C + SP + +L E+EE+ ++LSDI +D TR L+L+NQQRLAEME+S L +L++ + LE EK + LL MLP VA QL GK+V+A +F+ CTILFSD+VTFT+I SQC P+ +V MLN++Y FD+ + + YKVETIGDAYMVV GVP S H E +A A+ M+ AS +V +P++G +QIRVG+HSGPV+AGVVGEKMPR+CLFGD VN ASRMESHG+P +IH S + Y L + E RGE+++KGKG M+TYFL+
Sbjct: 7 LKLFGEYFFEFCKRSGYDHMMRTLGANLFEFIENLDALHGYLSISYKEMNAPSFRVEKNSDGTMLLHYYSDRRGLCHIVPGIIGAVAKDFFNSRIEMKIVNQMEEVERTGK-KEHVVFLITQHFPDKICSKSTSSPSQQTQKPSGCRHNKVHINKQVGDEYNLFFSGKLLRGFEPVYPNELCIDLKTFCNAFPFHIVFDEELRVRQAGVAVQKIVPGL---QTMGIQLDQYFTILHPEVTFTIASIRKFINSHF---VLQTRREMMPESWKDTPML---ECCGWPLSAACSTRASPLLRSLQELEERRMYLSDIAPHDATRHLILLNQQRLAEMELSSQLERKKEELRQLSQHLEQEKQKTENLLYAMLPKHVANQLKEGKKVEAGEFKECTILFSDVVTFTTICSQCEPIQIVIMLNSMYLKFDRLTTVHNVYKVETIGDAYMVVGGVPIPVSSHGERVANFALGMIIASKEVTNPITGGPIQIRVGLHSGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGLPDKIHVSPNVYYALRDKGFQLEERGEIDVKGKGRMRTYFLV 561
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: gucy1b1 (guanylate cyclase 1 soluble subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-090313-160]) HSP 1 Score: 294.664 bits (753), Expect = 1.100e-90 Identity = 183/551 (33.21%), Postives = 297/551 (53.90%), Query Frame = 2
Query: 374 FGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKN---------LTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFD---KASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
FGK FF++ + GY ++ LG ++R FL+NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI+ K+I ++ H+ +I+ K + +LD F ++ ++ P+H++FD L + Q G + ++ L Q V LS + SL+ P + F I IN+ F N LT S + L+GQ+ L + + LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T+ L + +LPP VA +L + + V A+++ TILFS IV F + AS + +V++LN +Y+ FD + ++ YKVET+GD YM V+G+PE + HA+ I A+DM+E + +V+ V + +QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L ++E + E RG + +KGK MK +FL
Sbjct: 2 FGKMFFEFCQESGYDTILRVLGSNVREFLQNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVIQQKSEEC------DHIKFLIEEKDSEEEAFYEDLD-----GFEENGTQETRISPYTFCKAFPFHLMFDKDLMLTQCGNAIFRV---LPQLQPGVCNLSSVFSLVRPHIDFSFHGILSHINTVFVLRSKEGLLNVEMAECEDELTGTEISCLRLKGQMISLPETENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDLLPHP----------IITPTTPLLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASAEGAIKIVNLLNDIYTKFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAQSICHLALDMMEIAGQVK--VDEEPVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQSVENADPQFNLEYRGPITMKGKKEPMKVWFL 526
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000045584.1 (soluble guanylate cyclase 88E-like [Source:NCBI gene;Acc:103027224]) HSP 1 Score: 301.212 bits (770), Expect = 2.397e-90 Identity = 204/679 (30.04%), Postives = 347/679 (51.10%), Query Frame = 2
Query: 155 LTLSMLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQF-----KIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQ-HDVILLSK-----IASLIV--PAVPFDAVSIR----------RFINS----------------AFTFLLFRNYK--NLTNETDSG------ILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISK-HLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNGERKIP---VENVSQCNAA 2032
L++SM G + + + + + G++ W+ + E A+ F+ Y +S+ + +A + +E + ++G F + ++GY ++++ LG +R F+ LD LHEY + + P+F ++S + LHY S RKG + G L + K+F+ T+I +++ + F IQ + E + L I+++F P++I+F + V G L + L ++ +D LL++ ++I+ P F+ +S R NS AF ++ +YK N N +S + L+GQ+ ++ + + +F+G+P + +LS M + L+++D+ ++D +R+L+L Q+ E++ + + +++L+++ +L+ E + + LL +M+P VA++L G+ E F TILFSD+V FT I S PM VV MLN +Y+LFD SE + +KVETIGDAYMVVAG PE T HA I + A+DMV + + ++ P +G ++QIRVGIHSG VVAGVVG KMPR+ L GD V+ AS MES+G I S +TY+ L ++FE RG + IKG ++TY+L G+ N + P + +S+ A+
Sbjct: 31 LSVSMYGLLCESLHDFIKESYGDDVWKLVRERADV-RLHSFVTHQVYSESVIPRIAKAASGVTGTPYNELMNSWGVYFLGFVGKYGYDRILKVLGRHVRDFVNGLDNLHEYLRFSYPKVQPPTFFCQEESATGVTLHYRSKRKGYLHYAMGQLRQMGKQFYDTDIHVEVLSEQLVGDYSHVTMRLNFDNSAYRYIQKEDEEEQEILP-----ITSDF--------------FFEVFPFNIVFRQDMVVHNVGSGLATVFPDLDGKKINDAFLLARPLVEFTWNMIISHPNNLFEIMSKEPVKRERNLHNRVQNSDYENANRSADVDVELMAFQSIIGDDYKDGNSANAMESWGDGSRCLKLKGQMRYMPEWESIIFLGTPVMESLSAMFKTGLYINDLSMHDSSRDLVLAGTQQSEELKRALIQEQKKSSKLEESMKMLDYEMKKTDDLLYRMIPKPVAKRLRKGEPAVNTCEVFPDVTILFSDVVGFTRICSHITPMQVVSMLNTMYTLFDTLSEKHRVFKVETIGDAYMVVAGAPEKTKYHAHNICDMALDMVRSIDHLKDPSNGNNIQIRVGIHSGMVVAGVVGHKMPRYGLHGDTVHTASAMESNGKEMHIQLSSATYEHLKGSHFIFERRGTITIKGNVEIETYWLKGKRDKDGNAQAACPQFEAQTISKAAAS 689
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: gucy1b2 (guanylate cyclase 1, soluble, beta 2 [Source:ZFIN;Acc:ZDB-GENE-130530-664]) HSP 1 Score: 340.502 bits (872), Expect = 6.342e-111 Identity = 182/363 (50.14%), Postives = 243/363 (66.94%), Query Frame = 2
Query: 875 LYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLG 1963
L V+Q+G +QK L+ I L + ++ P V F SIR+FINS F R + + LRGQ+ +++ + L++ SP + +L E+EE+ L +SDI +D TR+L+L+NQQRLAEME+S L +L+ + L+ EK + LL MLP VA QL GK+V+A +F++CTILFSD+VTFT+I + C P+ +V MLNA+Y FD+ + + YKVETIGDAYMVV GVP HAE +A A+ MV A+ +V +PV+G ++QIRVGIHSGPV+AGVVGEKMPR+CLFGD VN ASRMESHG+PGRIH S ST + L E+ E RGE+E+KGKG M TYFLLG
Sbjct: 1 LRVKQAGVNIQKFVPGLQTMD---IRLDEYFTIGHPEVTFTIESIRKFINSQFVLKTRREMLPEARRSQPMLDLRGQMVWMEGSRSMLYLCSPLLRSLEELEERQLHISDIAPHDATRDLILLNQQRLAEMELSTQLERKKEELRVLSKNLQVEKKKTEALLYAMLPHHVANQLKEGKKVEAGEFKVCTILFSDVVTFTNICAVCEPIQIVHMLNAMYMRFDRLTTVHNVYKVETIGDAYMVVGGVPIPVPSHAERVANFALGMVLAAREVTNPVTGSAIQIRVGIHSGPVLAGVVGEKMPRYCLFGDTVNTASRMESHGLPGRIHLSPSTREALQGKEFDVEERGEIEVKGKGRMSTYFLLG 360
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002650.1 (pep scaffold:Pmarinus_7.0:GL478983:4063:28109:1 gene:ENSPMAG00000002416.1 transcript:ENSPMAT00000002650.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 202.601 bits (514), Expect = 1.937e-57 Identity = 112/239 (46.86%), Postives = 145/239 (60.67%), Query Frame = 2
Query: 1274 SKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVP-EVTSRHAEIIAEQAMDMVEASNKVRSPVSGK-SLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLLGRGVTHK 1981
SKHL + + + L EK + + LL MLP ++A L G+ KA+ + T+ FSDIV FT ++S P +VVD LN LY+ FD ++ YKVETIGDAYMVV+GVP E ++HA A A+D+V P + L+IR GIH+GPVVAGVVG KMPR+CLFGD VN ASRMES +I CS ST L + Y+ RG V +KGKG M T++L GR + K
Sbjct: 267 SKHLESVVTERTQDLVL---EKQKTDRLLYSMLPKQIADDLRQGRVTKAQSYASATVFFSDIVGFTQLSSSSTPYEVVDFLNKLYTSFDGIIDNYDVYKVETIGDAYMVVSGVPTENHNQHAVETANMALDLVNVCQAFVIPHKPEMQLKIRAGIHTGPVVAGVVGTKMPRYCLFGDTVNTASRMESTSEALKIQCSPSTSSILQQIGSYLLSCRGTVTVKGKGEMITWWLEGRDTSTK 502
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006352.1 (pep scaffold:Pmarinus_7.0:GL477176:19246:83539:-1 gene:ENSPMAG00000005587.1 transcript:ENSPMAT00000006352.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 207.223 bits (526), Expect = 7.119e-57 Identity = 115/240 (47.92%), Postives = 160/240 (66.67%), Query Frame = 2
Query: 1256 LAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSR-HAEIIAEQAMDMVEA--SNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-YVFEGRGEVEIKGKGLMKTYFLLG 1963
L+ ME ++ N + +++ T EK + LL Q+LP VA+QL G+ V+AE F TI FSDIV FTS++++ PM VV +LN LY+ FD ++ YKVETIGDAYMVV+G+P + +A+ IA ++ +++A + K+R K L++R+GIH+GPV AGVVG KMPRFCLFGD VN ASRMES+G +IH S +T + L + E RG+VE+KGKG M+TY+LLG
Sbjct: 583 LSRME--QYANNLEELVEERTQAYLEEKRKAETLLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTSLSAESTPMQVVTLLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVRNGKLNAKEIARMSLALLDAVKTFKIRHRQEDK-LKLRIGIHTGPVCAGVVGLKMPRFCLFGDTVNTASRMESNGEALKIHVSQTTKEVLDEFGCFRLELRGDVEMKGKGKMRTYWLLG 819
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: NPR1 (natriuretic peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:7943]) HSP 1 Score: 200.29 bits (508), Expect = 1.472e-56 Identity = 113/244 (46.31%), Postives = 152/244 (62.30%), Query Frame = 2
Query: 1256 LAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVP-EVTSRHAEIIAEQAMDMVEASN--KVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLLGRGVT 1975
L+ ME ++ N + +++ T EK + LL Q+LP VA+QL G+ V AE F TI SDIV FT++++Q PM VV LN LY+ FD ++ YKVETIGDAYMVV+G+P +RHA +A A+ ++ A +R + L +R+GIH+GPV AGVVG KMPR+CLFGD VN ASRME+ G RIH S +T L +V + G+V+IKGKG M+TY+LLG T
Sbjct: 266 LSRME--QYANNLEELVEERTQAYLEEKGKAEALLYQILPHSVAEQLKRGETVPAEAFDSVTIYLSDIVGFTAMSAQSTPMQVVAFLNDLYTCFDAIIDNFDVYKVETIGDAYMVVSGLPVHNGTRHAIEVARMALALLSAVRCFHIRHR-PAQRLLLRIGIHTGPVCAGVVGLKMPRYCLFGDTVNTASRMEAKGEAHRIHLSSTTKAILDEYGGFVVQLHGDVDIKGKGTMRTYWLLGENPT 506
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007957.1 (pep scaffold:Pmarinus_7.0:GL476724:304294:325245:1 gene:ENSPMAG00000007197.1 transcript:ENSPMAT00000007957.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 177.563 bits (449), Expect = 2.180e-51 Identity = 97/223 (43.50%), Postives = 138/223 (61.88%), Query Frame = 2
Query: 1304 LQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE---YVFEGRGEVEIKGKGLMK--TYFL 1957
L++T LE EK R LL + P VA+ L G + A KF T+LFSDIV FT+I +QC P+ V+ MLNALY+ FD YKVETIGDAY V G+ + ++ HA+ +A A+ M++ + KV +P G+ +++R+G+HSGPV+AGVVG MPR+CLFG+ V +A+R ES P R++ S +T++ L E + RGE+ G + YFL
Sbjct: 2 LEQTRRALEEEKKRTVDLLDSIFPGDVARCLWQGLAMPARKFDDVTVLFSDIVGFTAICAQCTPLQVIGMLNALYTRFDHQCGLLDIYKVETIGDAYCVAGGLHKPSAEHAQRVAWMALRMIDLAQKVLTP-DGEPIKMRIGLHSGPVLAGVVGVNMPRYCLFGNNVALANRFESGSHPCRVNVSPTTHRLLSGDEGFRFTPRSRGELPANFPGDVPGICYFL 223
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO47086 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN72]) HSP 1 Score: 386.726 bits (992), Expect = 8.470e-126 Identity = 224/590 (37.97%), Postives = 335/590 (56.78%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCD-NSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQ---------SKIEAQ-YGSLAELDKSISTNFRPKYPH-KSLVNARHMVNFL------PYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTN---ETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIAS--QCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDST 1867
M G IH + +LV+ K GEEAW+ I++ A + + +L+ Y D L+ A L D LE FG FF Y ++ GY ++++ LG ++R FL NLDALH++ A M APSF + N ++LHY S R GL + G++ IAK+ +TE+ I + N H+ I+ SK+ Q + S E +K N R K KS + + +N L P+H++FD L+++Q+G L ++ K + +D + + + + P + F ++ IN+ F L + TD+ + L+GQ+ ++ D+DC LF+ SPR+ + + EK L+ SDIP++D TR+LL ++ R AE E+ + L E +N L+K S L K R + LL +LP +VA +L + V AE ++ TILFSDIV FT++ S + PMD+V MLN LY+ FD S N YKVETIGDAYMVV G+P HA+ +A + M+EA++ V SPV + L+IR+GIH+GPV+AGVVG+KMPR+CLFG+ V +A++MES PGRI+ S T
Sbjct: 1 MYGFIHCALADLVIGKFGEEAWKDIIKKAGVELDGGSYLIHKIYDDEETLRLVGAACEATGLELDTVLEVFGAHFFAYCEKSGYDRILKVLGRNLRDFLCNLDALHDHLATIYPGMEAPSFRCTETDNGTLLLHYYSKRPGLSYIVIGLVKAIAKQLLETEVNVTIHQDIDEEN------DHVTFAIREAENKHSSGSKLGLQTFASGHETEKE--ANKRSKDASVKSETHKKREMNLLSFCKAFPFHVMFDRKLHIKQAGLSLTRIM--TKQKLNDTLKFTDLFRISRPRMEFSFQAVISHINTVFVVTTKAGAVQLPDNIESTDASLRLKGQMLYVPDSDCLLFLCSPRVKNMDSLREKGLYFSDIPVHDATRDLLFLSHARRAERELVEKLEETSNNLKKVESKLIEHKKRTDDLLYSILPKEVAAKLRLNQSVPAESYKTVTILFSDIVGFTALCSNDKIVPMDIVKMLNRLYTYFDMLSGMNDVYKVETIGDAYMVVGGLPHPCEDHADRVASMGLGMMEAADVVLSPVDREPLKIRIGIHTGPVMAGVVGKKMPRYCLFGNTVTLANKMESGTQPGRINISIDT 580
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO47083 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN69]) HSP 1 Score: 354.755 bits (909), Expect = 2.659e-111 Identity = 209/625 (33.44%), Postives = 345/625 (55.20%), Query Frame = 2
Query: 173 GHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSI-DKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFL-----LFRNYKNLTNET-------DSGIL-LRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASED--NGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKGLMKTYFLLGRGVTHKN 1984
G ++ ++ LV+ G WE I AA + FL Y D + ++L+ A L++ LE FG+ FF + + GY ++++ LGG +R F+ NLDALH++ M APSF + D++S+ +ILHY S R GL P + G++ +A++ TE+ +++ + + +F + ++Q + + S + PK + A P+H++F+ L V Q+G + ++ L D +S+I LI P + F +I IN+ F L + N+ N+ DS L L+GQ+ ++ ++D +F+ SP + L ++ + L+LSDIPL+D T++L+L+++Q AE +++ L +T++L++T + L +K + LL +LPP VA +L + + V+AE+ +M T+LFS IV F I PM++V +LN +Y+ FD ++ N YKVET+GD YM V+G+PE + HA I A+DM + + G +QI++GIHSG VVAGVVG K PR+CLFG+ VN+ SR E+ G PG+++ ++ TY+ LL E +VFE RG V +KG+ +LL R H++
Sbjct: 101 GFVNHALELLVVRNFGVAKWEEIKRAAELEIEGHFLQRIIYDDVMTYSLVGAATRVLDIETSAILEKFGEMFFDFCQESGYDRILQVLGGTLRDFIGNLDALHDHLGSIYPGMRAPSFRVSDRESDGALILHYYSERDGLEPIVVGIVKTVARKLLNTEVSVEVVKGKEYDGDDVQFVIKEISDRGRDCDSQVKNYDPIKDSFQLSNEPKISPATFCKA------FPFHLMFNRDLKVTQAGESVSRVVPSLTPGDAD---MSEIFELIRPQLRFTYEAIIAHINTVFVLRTREGKLSKPANNMCNKNGCCKLCWDSLPLRLKGQMVYIPESDNMIFLCSPSVGNLEDLADVGLYLSDIPLHDATKDLILLSEQFKAEYVLTQQLEILTDKLRQTHNALAEKKQLTDQLLYSVLPPSVANELRHNRPVQAERAEMVTLLFSGIVDFAQICKNSKPMEIVMLLNNIYTKFDMLTDPSINDVYKVETVGDKYMAVSGLPERCAFHARSICNMALDMKHVTQSIIH--KGDKIQIKIGIHSGEVVAGVVGHKTPRYCLFGNTVNLTSRTETTGAPGQVNITEFTYRCLLEPECQDPSFVFERRGPVAMKGRQEPMVTYLLTRDPRHRS 714
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO47084 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN70]) HSP 1 Score: 333.183 bits (853), Expect = 1.348e-104 Identity = 208/619 (33.60%), Postives = 341/619 (55.09%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCD--NSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKS-NCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFR--PKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LLFRNYKNLTNETDSGIL------LRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCN-PMDVVDMLNALYSLFDKASEDN-GAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL---EYVFEGRGEVEIKGKG-LMKTYFL 1957
M G ++ +++LV+ G + W+ ILE AN D +SR+F Y D +LL + L+ + LE FG FF++ GY MI +LGG++ F +LD LHE + M PSF + + N ++ L Y S R GL + G++ +A + + ++ ++ + ++ + + F ++ Q + E +Y + D ++ + S + P+H +FD + + Q+G LC L + L+ I I P + +I ++IN + LL + +E D G L RGQ+ +L + + +F+ SP ++ L + EK L++SDIP++D TR+L+L+++Q AE ++S+ L +T++LQ+T+ LENE+ + LL +LP VA L K V A K+++ TI+FS IV FT+ ++ PM++V+MLN +Y+ FD+ ++ N YKVET+GD YM V+G+P +HA IA A+DM++ S +++ G LQ+ +GIHSG VVAGVVG+K PR+CLFG+ VN+ SR E+ G+ G+I+ S+ Y+ LL ++VF+ RG V +KGK M TY L
Sbjct: 1 MYGFVNRALQDLVIQGYGPDVWKEILEKANLDLGDSREFNERRIYDDQNTSDLLIVAEDVIGLSKNAILERFGDAFFEHCIGSGYDSMIRSLGGNLMDFFNSLDGLHEQLLLLYPGMRPPSFRAKQHNINMKLELVYTSERSGLEYMVVGLVKTVASKLYGIQVNVELASMSSKDQSWAMF----YVLPQHQDEMKY--ILPTDTTLERTMKELSIMTLSSRIGTAAFCRACPFHAVFDQRMVIYQAGI---SLCRVLPAVRVGKSKLTDIFESIRPPIKLTFDNILQYINKVYVIKTKSGLLDSASLSTVSEDDYGNLESPSMRFRGQMMYLPECNSIMFLASPSVVNLDGLNEKGLYMSDIPIHDATRDLILLSEQHNAEFKLSQRLEILTDKLQQTSRELENEQQLNDRLLYSILPASVANDLRLRKPVNANKYEIVTIMFSGIVDFTNYCNKIKEPMEIVEMLNEVYTEFDQLTDSNDDIYKVETVGDKYMAVSGLPTRCDKHALNIANLALDMIDISKGMKA--KGIQLQVTIGIHSGEVVAGVVGQKKPRYCLFGNTVNLTSRTETTGLKGKINVSEYAYRCLLVQPHEQFVFKKRGPVVMKGKKEPMITYIL 608
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO43095 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZH8]) HSP 1 Score: 313.923 bits (803), Expect = 2.482e-97 Identity = 212/642 (33.02%), Postives = 322/642 (50.16%), Query Frame = 2
Query: 200 LVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVN--------ARHMVNF---LPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLT-----------------------------------NETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLS-----DIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVS-GKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLG 1963
L+ + GE+ W +I E A F + + Y DSIF L+ + + LE+ G F + + GY K++ LG +R FL LD LHE+ T + APSF +++ M LHY SSRKG ++ G + + K F+ +I+ +++ + ++ V ++Q + + IS RPK S+++ + H+ F P+H+++D + + G L+ + L + ++ SLI P + F S+ F+++ F L K L+ E DS I L+GQ+ ++D+ ++ +P ++ + FL D+ L + +E+ ++ + + M+ S L E +L +NEK R + LL QM+P VA +L G+ E F TILFSD+V FT+I S PM VV MLN +Y+ FDK SE + YKVETIGDAYMVV+G+PE T HAE +A+ A++M+ A ++ P L+IR+GIH+G VVAGVVG KMPR+CLFGD VN ASRMES G+ IH S+ T +FL Y + RG ++IKGKG MKTY+LLG
Sbjct: 13 LLTSRYGEDTWHKIRENAGV-QQHTFAIHNIYSDSIFQRLVSSAEEITGEDRNSLLESTGALFVSFIGQFGYDKILRILGRHIRDFLNGLDNLHEFMRFTYPKLKAPSFHCSEETERGMKLHYRSSRKGFLYYVVGQIREVGKMFYDQQIEVEVL-------EQHESKEGFVAVMQLYFDNR--------AFISARKRPK--RHSVISLGGETDSLSIHIDVFYEVFPFHVVYDKSMTITSVGSSLEAVLPNLVGCK-----ITDEFSLIRPFLDFSWESV--FVHTNVVFELQSRSKALSMRKNGKVQNGLLMGCPIMDALGEGVNGNHDNNHNVTEFDSRIHLKGQMMYIDEWKAMAYLATPINISTLMLINFTFFLQTYLDVDVILLFLRKEIRIILSKDVKCMQKSAKLEESMREL-------DNEKLRTDALLYQMIPKTVADRLRKGEPALNTCETFSNVTILFSDVVGFTTICSLIAPMQVVTMLNCMYTTFDKLSEKHNVYKVETIGDAYMVVSGIPERTKYHAEHVADMALNMLSAMPEIEDPAGLNDHLRIRIGIHTGTVVAGVVGLKMPRYCLFGDTVNTASRMESTGLAMAIHVSEDTQKFLAKANYALKQRGSMDIKGKGGMKTYWLLG 622
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Match: EDO40362 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7B2]) HSP 1 Score: 279.256 bits (713), Expect = 1.171e-84 Identity = 186/609 (30.54%), Postives = 310/609 (50.90%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASEL-----NLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFK-TEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPH------KSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF------RNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLN-EMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDA-YMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIK 1927
M G + ++ ++ K GEE W I+ A +S +F+ Y ++ F + A + L ++T D+ + FG F K+ +GY +I G R FL +D+LHE+ M +P+F D++++ + LHY S RKG ++ G + IA++F+ + I KI+ + + + SR + H+V ++ E ++P P K V+ + P+ ++ + + G LQ L L +L I + + P + + F L R + + + LRGQ+ F+ D D FLFI SP I L M + L+++D+ ++D ++E+ L + L ++E ++ E +L++ LE E+ R LL +M+P +A++L G E+F T+LFS + FTSI ++ N M V ++N L+ FD +E + YK ET+GDA YM V+GVP T HAE +A A+DM++A V+ P++ K + I VGIHSGPV AG+VGE+ ++CLFGD VN+ASR+ + +P RIH S++ + L +++ E RGE+E+K
Sbjct: 1 MYGLLLESIRHFIVGKYGEELWSEIVNIAGT-SSHEFVTHRLYDENWIFKIANAATTVLGDLDEHITVDDCMRLFGVCFVKFFSFYGYDPIIRVSGRHFRDFLIGIDSLHEHMRFGYPKMKSPTFYCDEETSTGINLHYESRRKGFMFYVVGQVEEIARKFYGISNINVKILKNESSQS--SREQCHIVYRLEFNNEG---------------YKPPPPDFLTVGTKKAVSTEVFFSIFPFSLVISPDMTLSMFGNGLQSLLGKL-------VLGRDIREVFILRRPKTDFTWHTLLTRKVVFELLSKVPLIRRPQPKHQGSTMTLHLRGQMKFMHDWDKFLFICSPLIANLDTMLQSGLYMNDLSMHDTSQEMALSGIKPLPQLEYARDQQFEQGIELEENMRKLEEERQRSEDLLYRMVPKPIAERLKRGGNPIDTCEEFDAATVLFSYMDGFTSICTRLNAMQAVTLINNLFIKFDLLTEKHNVYKFETLGDALYMAVSGVPVRTEHHAEPMAAMALDMLKAVQLVKDPITKKPMTITVGIHSGPVAAGLVGERTLQYCLFGDTVNIASRLRTTALPMRIHISENCRECLEESDFITEFRGEIELK 584
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: gucy1b1 (fatty acid binding protein 2 [Source:NCBI gene;Acc:101170642]) HSP 1 Score: 300.056 bits (767), Expect = 2.946e-92 Identity = 194/578 (33.56%), Postives = 311/578 (53.81%), Query Frame = 2
Query: 296 PDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRT-FLENLDALHEYFAVTQKSMIAPSF-SIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTF-----LL----FRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSI----ASQCNPMDVVDMLNALYSLFD---KASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-----YVFEGRGEVEIKGKG-LMKTYFL 1957
P IF ++ L++ +D +A K HG H NLG T + +NLDALH++ M APSF D + +ILHY S R+GL + G++ +A++ TEI+ K+I ++ H+ +I+ K + +LD F ++ ++ P+H++FD L + Q G + ++ L Q +L + SL+ P + F I IN+ F LL N LT S + L+GQ+ +L + + LF+ SP +M L ++ + L+LSDIPL+D TR+L+L+ +Q E ++++ L +T++LQ T LE+EK + + LL +LPP VA +L + + V A+++ TILFS IV F + AS + +V++LN +Y+ FD + ++ YKVET+GD YM V+G+PE + HA+ I A+DM+E + +V+ V +S+QI +GIH+G VV GV+G++MPR+CLFG+ VN+ SR E+ G G+I+ S+ TY+ L E + E RG + +KGK MK +FL
Sbjct: 7 PGVIFLEIIWLFGLLLHVVSDNRNQALLKVL------HGVHD--HNLGSVRWTEWFDNLDALHDHLGTIYPGMRAPSFRCTDAEKGNNLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIEMKVIQQKSEEC------DHIKFLIEEKDSEEEAFNEDLD-----GFEENGTQETRISPYTFCKAFPFHLMFDKDLMLTQCGNAIYRVLPQL---QPGSCILPSVFSLVRPHIDFSFHGILSHINTVFVLRSKEGLLNVETVENEDELTGVEISCLRLKGQMIYLPEAENILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQHTLRALEDEKKKTDRLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNTFCSKHASAEGAIKIVNLLNDVYTRFDILTDSRKNPYVYKVETVGDKYMTVSGLPEPCTHHAKSICHLALDMMEIAGQVK--VDDESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLQCAENADPQFQLEYRGPITMKGKKEPMKVWFL 560
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: gucy1a2 (guanylate cyclase 1 soluble subunit alpha 2 [Source:NCBI gene;Acc:100049248]) HSP 1 Score: 235.343 bits (599), Expect = 2.917e-66 Identity = 136/371 (36.66%), Postives = 210/371 (56.60%), Query Frame = 2
Query: 773 SISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILLSKIASLIVPAV--PFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL 1879
S+STN P + P+H++ + + Q G L++ + E H ++ P + F + +R + S FT + R + + L+GQ+ + ++ +F+GSPR+ L E+ + L+LSDIP++D TR+++LV +Q A+ + K ++++ L++T LE EK R LL + P VAQ+L G+ V A KF T+LFSDIV FT++ + C PM V+ MLN LY+ FD YK+ETIGDAY V G+ + HA+ IA A+ M+E S++V +P GK +++R+GIH+G V+AGVVG KMPR+CLFG+ V +AS+ ES P I+ S +TYQ L
Sbjct: 378 SLSTN-----PSDLRIGLATFCRAFPFHLVLGPHMELLQLGEGLRRQA---RIEPHRSFSFRDCFEIVSPKMEPSFQGILLR--LASPFT-IRTRPDSTQAGTKEKVMELKGQMIHVPESCSLMFLGSPRVDKLEELMGRGLYLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKRRTVDLLYSIFPGDVAQKLWQGQPVPARKFDDVTMLFSDIVGFTAVCAHCTPMQVISMLNELYTRFDYQCGILDVYKIETIGDAYCVAGGLHKKVDSHAKPIAHMALKMMELSDEVLTP-DGKPIKLRIGIHTGSVLAGVVGVKMPRYCLFGNNVTLASKFESGSHPRCINVSPTTYQLL 736
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: gucy2g (guanylate cyclase 2g [Source:ZFIN;Acc:ZDB-GENE-130531-70]) HSP 1 Score: 218.394 bits (555), Expect = 1.217e-59 Identity = 117/236 (49.58%), Postives = 156/236 (66.10%), Query Frame = 2
Query: 1268 EISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVP-EVTSRHAEIIAEQAMDMVEASNKVRSP-VSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL-LALEYVFEGRGEVEIKGKGLMKTYFLLGR 1966
++ K+ N + +++ T+ L EKAR + LL MLP +A QL++GK V+ + + M TI FSDIV FTS+ S + M+VV LN LYSLFD + YKVETIGDAYMV +G+P ++HA I+ A+ + A + P + + L IR+GIHSGPVVAGVVG MPR+CLFGD VN ASRMES+ +P +IH S ST L LA + E RGE+E+KGKG KTY+LL +
Sbjct: 705 KLEKYANHLEEVVEERTNQLTVEKARADKLLSSMLPRYIADQLMSGKSVEPQSYDMVTIFFSDIVGFTSMCSVSSAMEVVTFLNDLYSLFDDIIKMYDVYKVETIGDAYMVASGLPISNGNKHALEISTMALHFLAAIKIFKIPHMPNEGLAIRIGIHSGPVVAGVVGTTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILVLAGSFEMEERGEIEMKGKGFQKTYWLLKK 940
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc-r2 (guanylate cyclase OlGC-R2 [Source:NCBI gene;Acc:100216004]) HSP 1 Score: 216.083 bits (549), Expect = 1.977e-58 Identity = 117/238 (49.16%), Postives = 158/238 (66.39%), Query Frame = 2
Query: 1268 EISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTS-RHAEIIAEQAMDMVEA--SNKVRSPVSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFL--LALEYVFEGRGEVEIKGKGLMKTYFLLGR 1966
+ S +L E+ +++ T LE EK + LL QMLPP VA+ L+ G VK E F ++ FSDIV FT+I++ P++VVD+LN LY+LFD ++ YKVETIGDAYMV +GVP + RHA IA ++D++ A + K+R + ++IR+G+H+GP VAGVVG MPR+CLFGD V ASRMES GMP RIH +S + L L L Y E RG E+KGKG +TY+L+GR
Sbjct: 837 QYSSNLEEL---IRERTEELEIEKQKTEKLLTQMLPPSVAEALMVGGTVKPEYFDSVSLYFSDIVGFTTISANSEPIEVVDLLNDLYTLFDAIIGNHDVYKVETIGDAYMVASGVPVPNANRHAAEIANMSLDILSAVGTFKMRH-MPDVPVRIRIGLHTGPCVAGVVGLTMPRYCLFGDTVTTASRMESSGMPYRIHVHESIVKVLKELNLGYKLELRGRTEVKGKGTEETYWLVGR 1070
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Match: olgc8 (membrane guanylyl cyclase [Source:NCBI gene;Acc:100049174]) HSP 1 Score: 211.46 bits (537), Expect = 1.614e-57 Identity = 118/237 (49.79%), Postives = 146/237 (61.60%), Query Frame = 2
Query: 1274 SKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRVKAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTS-RHAEIIAEQAMDMVEASNKVRSP-VSGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLAL-EYVFEGRGEVEIKGKGLMKTYFLLGRGVT 1975
SKHL + + + T L +EK + + LL MLP +VA L GK +A+ + T+ FSDIV FT ++S P VVD LN LY+ FD ++ YKVETIGDAYMVV+GVP+ HA IA A+D+V R P LQIR GIHSGPVVAGVVG KMPR+CLFGD VN ASRMES +I CS ST+ L + YV E RG ++KGKG M TY+L G+ T
Sbjct: 586 SKHLEVL---VAERTQDLTHEKQKTDRLLYSMLPKQVADDLRQGKPSQAQSYVSATVFFSDIVGFTQLSSSSTPYQVVDFLNKLYTTFDNIIDNYDVYKVETIGDAYMVVSGVPQENGILHASEIASMALDLVGVCQTFRIPHKPSMQLQIRAGIHSGPVVAGVVGTKMPRYCLFGDTVNTASRMESTSTALKIQCSSSTFYLLEEIGGYVLECRGVQQVKGKGDMVTYWLQGKKTT 819
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000035922.1 (SMESG000035922.1) HSP 1 Score: 337.806 bits (865), Expect = 1.497e-105 Identity = 223/691 (32.27%), Postives = 366/691 (52.97%), Query Frame = 2
Query: 155 LTLSMLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPES-----RFRQHMVIIIQSKIEAQYGSLAEL---DKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILL--SKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVS-GKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-YVFEGRGEVEIKGKGLMKTYFLLGRGVTHKNG--------ER-KIPVENVSQCNAAVKCNAKE----------KSRTAEI----------SNNHNDS 2095
+ L+M G I +++ + K GE+ W IL +A+ D F Y +++ N++E+ + LN+T DE + G F + ++GY ++ LG D+R FL LD LHEY + + PSF +S + L Y + R G +++G + IA++F+ TE+ +I++ +++N + F I I+ + E+ + + R K ++NA + LP+ +I + L R PL + D ++L + I LI + I R N + + K + + + +RGQ+ ++ + D +F+G+P + + M + LF++D+ ++D +R+L+L +Q+ AE++++ + +E + +L+++ L+ + + LL QM+P VA++L +G E F TILFSD+V FT+I S+ NP DVV+MLN LYS+FD+ +E + YKVETIGDAYM+ +G P VT HA I E ++DM+++ + P +SLQIRVGIH+G VVAGVVG KMPR+CLFGD VN ASRMES+ P +IH S++T + L+ Y RG ++IKGKG +KTY+L G + + ER +I + + + + +K + E +SRT I S NH DS
Sbjct: 1 MLLAMYGLILEGVRQFICSKYGEDHWNSILRSADID-VITFGTHEVYGETLIPNIIESASRFLNMTVDEIMFLNGIFFINFVSQYGYDGILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESKTGITLQYRTRRSGYIHYVRGQITEIARQFYSTEMSIEIVSQTSEDNVSNCIMRLHFNNKNFIQIEDLLPVPSEVFFEVFPFNIVFNRGMRAINCGKGMLNA---LPDLPHQLITEYFLLTR-----------PLIEFTWDAVMLHTNNIFELIHLDPKISSSKILREGNHS-------SKKRTLSRNNPSLKMRGQMKYMPEWDAIVFLGTPIMRDVESMYKAGLFINDLSMHDSSRDLVLAGEQQSAELKLALEQESEKSKKLEESLHKLDELMKKTDELLFQMIPKSVAERLRHGSEAVDTCEHFDEVTILFSDVVGFTTICSKINPFDVVEMLNKLYSVFDELTEKHKVYKVETIGDAYMIASGCPTVTQYHAPFIIEMSLDMIKSIGFLMDPSDHSRSLQIRVGIHTGMVVAGVVGLKMPRYCLFGDTVNTASRMESNSEPQKIHISEATKAKVTDLQSYKISERGHIDIKGKGNIKTYWLDGHNLENPKAVASLITQLERERITISDSNLAGSQIKGDGWESLTHSRASSARSRTGSILELSHTKRLASINHGDS 669
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000035922.1 (SMESG000035922.1) HSP 1 Score: 335.88 bits (860), Expect = 9.702e-105 Identity = 208/614 (33.88%), Postives = 339/614 (55.21%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPES-----RFRQHMVIIIQSKIEAQYGSLAEL---DKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQHDVILL--SKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVS-GKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-YVFEGRGEVEIKGKGLMKTYFLLG 1963
M G I +++ + K GE+ W IL +A+ D F Y +++ N++E+ + LN+T DE + G F + ++GY ++ LG D+R FL LD LHEY + + PSF +S + L Y + R G +++G + IA++F+ TE+ +I++ +++N + F I I+ + E+ + + R K ++NA + LP+ +I + L R PL + D ++L + I LI + I R N + + K + + + +RGQ+ ++ + D +F+G+P + + M + LF++D+ ++D +R+L+L +Q+ AE++++ + +E + +L+++ L+ + + LL QM+P VA++L +G E F TILFSD+V FT+I S+ NP DVV+MLN LYS+FD+ +E + YKVETIGDAYM+ +G P VT HA I E ++DM+++ + P +SLQIRVGIH+G VVAGVVG KMPR+CLFGD VN ASRMES+ P +IH S++T + L+ Y RG ++IKGKG +KTY+L G
Sbjct: 1 MYGLILEGVRQFICSKYGEDHWNSILRSADID-VITFGTHEVYGETLIPNIIESASRFLNMTVDEIMFLNGIFFINFVSQYGYDGILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESKTGITLQYRTRRSGYIHYVRGQITEIARQFYSTEMSIEIVSQTSEDNVSNCIMRLHFNNKNFIQIEDLLPVPSEVFFEVFPFNIVFNRGMRAINCGKGMLNA---LPDLPHQLITEYFLLTR-----------PLIEFTWDAVMLHTNNIFELIHLDPKISSSKILREGNHS-------SKKRTLSRNNPSLKMRGQMKYMPEWDAIVFLGTPIMRDVESMYKAGLFINDLSMHDSSRDLVLAGEQQSAELKLALEQESEKSKKLEESLHKLDELMKKTDELLFQMIPKSVAERLRHGSEAVDTCEHFDEVTILFSDVVGFTTICSKINPFDVVEMLNKLYSVFDELTEKHKVYKVETIGDAYMIASGCPTVTQYHAPFIIEMSLDMIKSIGFLMDPSDHSRSLQIRVGIHTGMVVAGVVGLKMPRYCLFGDTVNTASRMESNSEPQKIHISEATKAKVTDLQSYKISERGHIDIKGKGNIKTYWLDG 592
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000079311.1 (SMESG000079311.1) HSP 1 Score: 321.627 bits (823), Expect = 8.302e-100 Identity = 199/612 (32.52%), Postives = 325/612 (53.10%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELDKSISTNFRPKYPHKSLVNARHMVNFLPYHIIFDSMLYVRQSGYMLQKLC-DPLKDEQHDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLFRNYKNLT---------NETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAEMEIS-KHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNKVRSPVSG-KSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALE-YVFEGRGEVEIKGKGLMKTYFL 1957
M G + +++ + G +AW +ILE + D + F + Y + + ++ A +T DE + GK F + +GY ++ LG D+R FL LD LHEY + + PSF +S + L Y + R G +++G + IAK F+ T I+ +II K P+K + N P++I+F+ L V G ++ L D + D DV +++ P + +I N+ F +L K+ E++ + +RGQ+ ++++ D +F+G+P + + M + L+++D+ ++D +R+L+L +Q+ AE+ ++ + +E + +L+ + L++E R + LL QM+P VA +L NG++ E F TILFSD+V FT I S P+DVV+MLN++YS+FD+ +E + YKVET+GDAYM+ +G P +T+ +A +IAE A++M++ +++P + +SL+IRVGIH+G VVAGVVG KMPR+CLFGD VN ASRMES G +I S ST + L+ Y RG VE+KGKG + TY+L
Sbjct: 1 MYGLLLEGIRQFICSTYGLQAWTQILEKSKLDVNV-FAIHEVYDEELLKRIVFTAADYCRITKDELMFQNGKYFISFLSHYGYDSILRVLGRDLRDFLNGLDNLHEYLRFSYPKLKPPSFFCVNESRTGITLQYRTQRAGFVHYVRGQIFEIAKTFYNTTIEIEIIGEN-----------------------------------------KMPNK-------VFNCFPFNIVFNRGLRVVNCGRGMRNLIPDIIGDYVGDVFHVNR------PIIQISWDTIILHSNNVFELILLHIKKSRLSDSDSSNSLKESNYRLRMRGQMKYMNEWDAIVFLGTPIMRDVESMWKAGLYINDLSMHDSSRDLVLAGEQQSAELRLALEQESEKSKRLEDSLKRLDDEMRRTDELLYQMIPRAVANRLRNGEKSVNTCETFNEVTILFSDVVGFTKICSNITPLDVVEMLNSMYSVFDQLTERHKVYKVETVGDAYMIASGCPVLTTLNAPLIAEMALEMIDKICTIKNPANEIESLKIRVGIHTGTVVAGVVGIKMPRYCLFGDTVNTASRMESTGETQKIQISSSTQKKLMMHNVYQIVERGVVEVKGKGNVSTYWL 557
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000051217.1 (SMESG000051217.1) HSP 1 Score: 285.419 bits (729), Expect = 3.731e-84 Identity = 189/629 (30.05%), Postives = 313/629 (49.76%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHS-YPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELD-----KSISTNFRPKYPHKSLVNARHMV-NFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQ-HDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF----------------RNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAE-MEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNK-VRSPV-SGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGR 1966
M G + ++ V G + W + L A D + HS Y ++I ++ A++S LN ++ + G F +Y GY K++ LG + F++ LD LHEY + + PSF+++ + + LHY + R G +++G L IAK F++ +I+ I + SKIE Y + D K P+++ R M P+H++ L ++ G + +C L + +D +LSK P + I R+ NS F +L N K L++ I +GQ+ + + +F+G+P I + +++E LFL+D+ +D ++++L Q+ E ME+ + E + QL++ L + + + LL Q +P +A++L G + FQ +I FS +V F I S+ + +V++LN +Y+ FD +E YKVETI D+YM+V+G P T H+ I E A+ M+ + K +++P S S+++ +G HSG +VAG+VG KMPR+CLFGD VN +SRM SHG P ++H SD ++ + Y + RGE+EIKGKG M TYF+ R
Sbjct: 1 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS--INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIK---------------------IFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSK------PFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKQLEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESR 600
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Match: SMESG000051217.1 (SMESG000051217.1) HSP 1 Score: 285.034 bits (728), Expect = 6.011e-84 Identity = 189/629 (30.05%), Postives = 313/629 (49.76%), Query Frame = 2
Query: 167 MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHS-YPDSIFFNLLEAVASELNLTNDETLEAFGKQFFKYTKRHGYHKMIENLGGDMRTFLENLDALHEYFAVTQKSMIAPSFSIDKKSNCEMILHYNSSRKGLYPWIKGMLGCIAKEFFKTEIQFKIITSATDNNPESRFRQHMVIIIQSKIEAQYGSLAELD-----KSISTNFRPKYPHKSLVNARHMV-NFLPYHIIFDSMLYVRQSGYMLQKLCDPLKDEQ-HDVILLSKIASLIVPAVPFDAVSIRRFINSAFTFLLF----------------RNYKNLTNETDSGILLRGQICFLDDNDCFLFIGSPRIMTLSEMEEKNLFLSDIPLYDVTRELLLVNQQRLAE-MEISKHLNEMTNQLQKTTSLLENEKARVNMLLCQMLPPKVAQQLLNGKRV--KAEKFQMCTILFSDIVTFTSIASQCNPMDVVDMLNALYSLFDKASEDNGAYKVETIGDAYMVVAGVPEVTSRHAEIIAEQAMDMVEASNK-VRSPV-SGKSLQIRVGIHSGPVVAGVVGEKMPRFCLFGDAVNVASRMESHGMPGRIHCSDSTYQFLLALEYVFEGRGEVEIKGKGLMKTYFLLGR 1966
M G + ++ V G + W + L A D + HS Y ++I ++ A++S LN ++ + G F +Y GY K++ LG + F++ LD LHEY + + PSF+++ + + LHY + R G +++G L IAK F++ +I+ I + SKIE Y + D K P+++ R M P+H++ L ++ G + +C L + +D +LSK P + I R+ NS F +L N K L++ I +GQ+ + + +F+G+P I + +++E LFL+D+ +D ++++L Q+ E ME+ + E + QL++ L + + + LL Q +P +A++L G + FQ +I FS +V F I S+ + +V++LN +Y+ FD +E YKVETI D+YM+V+G P T H+ I E A+ M+ + K +++P S S+++ +G HSG +VAG+VG KMPR+CLFGD VN +SRM SHG P ++H SD ++ + Y + RGE+EIKGKG M TYF+ R
Sbjct: 1 MYGLLVGALQHFVESLYGADIWIQALNLAEIDYQS--INTHSVYAETIIPRIVNALSSILNEAEEDIMFHNGCYFVEYLSTCGYDKILRVLGRNFVDFIQGLDNLHEYLRFSYPKIKPPSFNVESVTESTIKLHYRTKRPGFQHYVRGQLVNIAKYFYEKDIK---------------------IFLISKIEDSYQTHYTFDILLDMKGFEKTLEISIPYENFPIRRDMFFEIFPFHLVLSENLTIQHCGVGFKHICPTLLNSNFNDEFILSK------PFIDATYEKIVRYNNSNFEIILMPHTSSGPLNAALKKDKANIKRLSSIVQGRIKFKGQMLDMPLWNGLMFLGTPVIRDVKQLKEAGLFLNDLNKFDTGKDIILAGSQQSDELMELFEKQLEKSKQLEENMRDLNKFRKKTDELLYQCIPKSIAKRLRKGVSSMDTCQVFQSVSICFSKVVDFVQICSEISVDKIVNLLNDMYTHFDNLTELYKVYKVETISDSYMLVSGAPSTTDLHSAHIVEMALSMITCAKKNLKNPAKSSGSIELLIGCHSGMIVAGIVGIKMPRYCLFGDTVNTSSRMMSHGSPNKVHVSDIFFEHISQFPYNYTKRGEMEIKGKGSMTTYFIESR 600 The following BLAST results are available for this feature:
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Guanylate cyclase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008588 ID=SMED30008588|Name=Guanylate cyclase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2228bpback to top protein sequence of SMED30008588-orf-1 >SMED30008588-orf-1 ID=SMED30008588-orf-1|Name=SMED30008588-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=660bp MLGHIHLCMKELVLDKMGEEAWERILEAANCDNSRDFLVFHSYPDSIFFNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
