EF-hand domain-containing protein

Overview
NameEF-hand domain-containing protein
Smed IDSMED30008542
Length (bp)791
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of EF-hand domain-containing protein (SMED30008542) t-SNE clustered cells

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30008542) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of EF-hand domain-containing protein (SMED30008542) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30008542) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008542

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30008542SMESG000045029.1 SmedASXL_009569SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.433e-15
Identity = 48/161 (29.81%), Postives = 74/161 (45.96%), Query Frame = 3 
Query:  246 LIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGI-------EYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNC---IKIYL-NAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
            L QM+ FI AF  ID DS+  I   EL  Y  K     + I  W+  FD D    I++EE+C+  G+       E      +R      +P N++I+ +TM + ++       K Y+ N   +G+  + +   +   L   YG  WQ  L+ G +W  FSH
Sbjct:    7 LSQMEEFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQFKEYVDNTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSH 167
[Close]
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P32070|SM21_SCHMA (Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=SM21.7 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.816e-14
Identity = 39/157 (24.84%), Postives = 71/157 (45.22%), Query Frame = 3 
Query:  255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIY---LNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
            M+ FI+ +  +  D    + T +LS+   K     + + +W+ +FD+D    IT EE+C+ LG++     ++R            S+PE V I+ STM + ++    +++    N +        ++K     L   YG  WQ   +   +W  FSH
Sbjct:    5 MEKFIQTYLTLLRDGDETVETSKLSESCRKEKLDMKQVNEWIALFDVDKDQKITFEEFCRGLGLKQNEMRIERNHIKTVQSGREQSLPEGVSIIASTMPKPKQVEVTQLFKDIYNEVKKDPDMNKVVKTFKSELERRYGRVWQVNAVTHSYWASFSH 161
[Close]
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267DRL5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010884g1 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 5.584e-28
Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 
Query:  243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
             +I+ + F++AF AID D S  I+ +EL +YM KN+++  FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR     +P  V ++ S+M E  +   ++    A       + I  D+ ++L   +G  WQ  ++ GQ++  +SH
Sbjct:   29 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVETVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 179
[Close]
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8GDT5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 6.122e-28
Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 
Query:  243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
             +I+ + F++AF AID D S  I+ +EL +YM KN+++  FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR     +P  V ++ S+M E  +   ++    A       + I  D+ ++L   +G  WQ  ++ GQ++  +SH
Sbjct:   26 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVETVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 176
[Close]
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.054e-27
Identity = 59/159 (37.11%), Postives = 92/159 (57.86%), Query Frame = 3 
Query:  243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYK--RASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713
            A+   D+FI+A+FAIDT+  GEI++ +L+ YM +N+Y   F++KWLK+FD DNSGTITL+EYC+ LG+E K  +A    +M   S+P ++ ++ S M    +   I+I  +        K + K + +         W   +I GQ W ++SH  G S 
Sbjct:    2 AVTMTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVKADYIAKMRSGSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSF 160
[Close]
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267G611 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010884g2 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 1.151e-27
Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 
Query:  243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
             +I+ + F++AF AID D S  I+ +EL +YM KN+++  FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR     +P  V ++ S+M E  +   ++    A       + I  D+ ++L   +G  WQ  ++ GQ++  +SH
Sbjct:   29 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVESVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 179
[Close]
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H025 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.209e-27
Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 
Query:  243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695
             +I+ + F++AF AID D S  I+ +EL +YM KN+++  FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR     +P  V ++ S+M E  +   ++    A       + I  D+ ++L   +G  WQ  ++ GQ++  +SH
Sbjct:   26 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVESVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 176
[Close]
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1)

HSP 1 Score: 318.546 bits (815), Expect = 1.524e-111
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3 
Query:  255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713
            MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL
Sbjct:    1 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 153
[Close]
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1)

HSP 1 Score: 114.775 bits (286), Expect = 2.071e-31
Identity = 59/160 (36.88%), Postives = 86/160 (53.75%), Query Frame = 3 
Query:  255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713
            MD+F+ AFF ID+D S EI+  EL  YM  N+ +  F+KKW+ IFD ++ G ITL+EYC VLG+    A + R            ++P++VKI+QS++        + I +N  S   +   + K + E L   YG  W C  + GQ W+YFS     S 
Sbjct:    1 MDNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSF 160
[Close]
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1)

HSP 1 Score: 112.079 bits (279), Expect = 2.228e-30
Identity = 63/158 (39.87%), Postives = 91/158 (57.59%), Query Frame = 3 
Query:  258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAIS-SGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713
            D+FI+A+  ID D SG I+ DEL  YM +N+Y+ +F+ KWL++FD DN+G ITLEE+C+ LG+E      KR +L+     +S+   V IL S M +      I++   A     +S K     + +NL L   T W C + KGQ+W Y+S    CSL
Sbjct:    3 DAFIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGLEVSDFIDKRTALKESAD-FSLYNGVNILSSNMVQPNEGIVIQVIRKARELYPNSFKEQSTYIKQNLDLVLETTWHCIITKGQYWAYYSCEPECSL 159
[Close]
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.175e-21
Identity = 47/152 (30.92%), Postives = 82/152 (53.95%), Query Frame = 3 
Query:  258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHL 698
            D+F+ AFF+ID  ++ +I+  +L ++ NKN+Y  +F+ KWL +FD + +G ITLEE+C VLG+E      KR   Q        P++ ++L   +++ +         N I + +  K I +         Y   W   +I+GQ+W ++S+L
Sbjct:    6 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEE--------RYQRAWVILIIRGQYWAHYSYL 149
[Close]
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.246e-21
Identity = 47/152 (30.92%), Postives = 82/152 (53.95%), Query Frame = 3 
Query:  258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHL 698
            D+F+ AFF+ID  ++ +I+  +L ++ NKN+Y  +F+ KWL +FD + +G ITLEE+C VLG+E      KR   Q        P++ ++L   +++ +         N I + +  K I +         Y   W   +I+GQ+W ++S+L
Sbjct:    3 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEE--------RYQRAWVILIIRGQYWAHYSYL 146
The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|P14202|TEGU_SCHMA1.433e-1529.81Tegument antigen OS=Schistosoma mansoni OX=6183 GN... [more]
sp|P32070|SM21_SCHMA8.816e-1424.84Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=S... [more]
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BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DRL55.584e-2835.10Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GDT56.122e-2835.10Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F2V41.054e-2737.11Dynein light chain (Fragment) OS=Macrostomum ligna... [more]
A0A267G6111.151e-2735.10Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H0251.209e-2735.10Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000045029.11.524e-111100.00SMESG000045029.1[more]
SMESG000026622.12.071e-3136.88SMESG000026622.1[more]
SMESG000006950.12.228e-3039.87SMESG000006950.1[more]
SMESG000026288.13.175e-2130.92SMESG000026288.1[more]
SMESG000026288.13.246e-2130.92SMESG000026288.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008542 ID=SMED30008542|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=791bp
GTGAATATGAATGTGTGCATCTCATTTACGCGTGGGCGAATATTCTGTCA
ACAATTAGGCAGCACTCTATCAAATGTGCCACACATTCTGTAACAGAATG
ACCTTTCAATTTTTTCTTCAATACTCTACGAAATTCGAATTTTTTGAATT
CTCAATATTCACAGATTTATCGTTGTTTTTAAAACTTGAAATTAAAGGAG
GAATTTTTTTAATCATTTTTCGGTTCTTTTTATTCACTTTAAGCGTTAAT
TCAAATGGATTCATTTATTGAGGCATTTTTTGCGATTGATACGGACTCTT
CAGGGGAAATTTCGACTGATGAGTTAAGCGATTACATGAATAAGAATCAT
TACAGATCAGAATTTATTAAAAAATGGTTGAAAATTTTCGATATTGATAA
TAGTGGCACCATCACGCTGGAGGAATACTGCAAAGTGTTGGGTATCGAAT
ACAAACGGGCATCTTTACAGCGAAGAATGTCGATGTATTCGATTCCGGAA
AATGTGAAAATTTTGCAGTCCACAATGGACGAGGGTCAGAGGAATAATTG
CATCAAAATATACTTAAATGCTATCTCGAGTGGAGATTCTCCAAAGGGAA
TATTGAAAGATGTCCATGAAAATTTAATTCTTTATTATGGAACTGGTTGG
CAGTGCTTTTTAATCAAGGGCCAGCATTGGACATATTTCTCTCATTTAGA
AGGATGCTCGTTGATTATTCATATAGACGGATTTATTTTTATCATTTTTA
GAACCGCAATCTAATTCTCCTGGAAATGATAGTCCTAGCTC
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protein sequence of SMED30008542-orf-1

>SMED30008542-orf-1 ID=SMED30008542-orf-1|Name=SMED30008542-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=170bp
MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNS
GTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCI
KIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEG
CSLIIHIDGFIFIIFRTAI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR037177DLC_sf
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR001372Dynein_light_chain_typ-1/2
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
Vocabulary: cellular component
TermDefinition
GO:0030286dynein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 37..65
e-value: 0.023
score: 23.9
coord: 3..31
e-value: 1.1
score: 17.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 6..61
e-value: 7.4E-9
score: 35.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 33..68
score: 11.64
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..28
score: 7.093
IPR002048EF-hand domainCDDcd00051EFhcoord: 4..62
e-value: 1.75289E-9
score: 49.4685
IPR037177Dynein light chain superfamilyGENE3DG3DSA:3.30.740.10coord: 74..168
e-value: 6.6E-6
score: 28.0
IPR037177Dynein light chain superfamilySUPERFAMILYSSF54648DLCcoord: 84..167
IPR001372Dynein light chain, type 1/2PFAMPF01221Dynein_lightcoord: 84..167
e-value: 6.2E-10
score: 39.3
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..67
e-value: 4.3E-13
score: 50.8
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 46..58
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 2..66