EF-hand domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008542 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1) HSP 1 Score: 76.2554 bits (186), Expect = 1.433e-15 Identity = 48/161 (29.81%), Postives = 74/161 (45.96%), Query Frame = 3 Query: 246 LIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGI-------EYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNC---IKIYL-NAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 L QM+ FI AF ID DS+ I EL Y K + I W+ FD D I++EE+C+ G+ E +R +P N++I+ +TM + ++ K Y+ N +G+ + + + L YG WQ L+ G +W FSH Sbjct: 7 LSQMEEFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQFKEYVDNTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSH 167
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P32070|SM21_SCHMA (Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=SM21.7 PE=2 SV=1) HSP 1 Score: 71.2478 bits (173), Expect = 8.816e-14 Identity = 39/157 (24.84%), Postives = 71/157 (45.22%), Query Frame = 3 Query: 255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIY---LNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 M+ FI+ + + D + T +LS+ K + + +W+ +FD+D IT EE+C+ LG++ ++R S+PE V I+ STM + ++ +++ N + ++K L YG WQ + +W FSH Sbjct: 5 MEKFIQTYLTLLRDGDETVETSKLSESCRKEKLDMKQVNEWIALFDVDKDQKITFEEFCRGLGLKQNEMRIERNHIKTVQSGREQSLPEGVSIIASTMPKPKQVEVTQLFKDIYNEVKKDPDMNKVVKTFKSELERRYGRVWQVNAVTHSYWASFSH 161
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267DRL5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010884g1 PE=4 SV=1) HSP 1 Score: 115.931 bits (289), Expect = 5.584e-28 Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 Query: 243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 +I+ + F++AF AID D S I+ +EL +YM KN+++ FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR +P V ++ S+M E + ++ A + I D+ ++L +G WQ ++ GQ++ +SH Sbjct: 29 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVETVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 179
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8GDT5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 115.546 bits (288), Expect = 6.122e-28 Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 Query: 243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 +I+ + F++AF AID D S I+ +EL +YM KN+++ FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR +P V ++ S+M E + ++ A + I D+ ++L +G WQ ++ GQ++ +SH Sbjct: 26 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVETVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 176
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1) HSP 1 Score: 114.39 bits (285), Expect = 1.054e-27 Identity = 59/159 (37.11%), Postives = 92/159 (57.86%), Query Frame = 3 Query: 243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYK--RASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713 A+ D+FI+A+FAIDT+ GEI++ +L+ YM +N+Y F++KWLK+FD DNSGTITL+EYC+ LG+E K +A +M S+P ++ ++ S M + I+I + K + K + + W +I GQ W ++SH G S Sbjct: 2 AVTMTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVKADYIAKMRSGSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSF 160
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267G611 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010884g2 PE=4 SV=1) HSP 1 Score: 115.161 bits (287), Expect = 1.151e-27 Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 Query: 243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 +I+ + F++AF AID D S I+ +EL +YM KN+++ FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR +P V ++ S+M E + ++ A + I D+ ++L +G WQ ++ GQ++ +SH Sbjct: 29 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVESVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 179
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H025 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 114.775 bits (286), Expect = 1.209e-27 Identity = 53/151 (35.10%), Postives = 92/151 (60.93%), Query Frame = 3 Query: 243 ALIQMDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSH 695 +I+ + F++AF AID D S I+ +EL +YM KN+++ FIKKWLK+FD+++ G I+L+E+C+VLG++ K A + RR +P V ++ S+M E + ++ A + I D+ ++L +G WQ ++ GQ++ +SH Sbjct: 26 PVIEANPFLDAFIAIDEDGSETITVEELRNYMKKNNFQESFIKKWLKMFDLNSDGVISLDEFCEVLGLQMKNARMYRRKVQGGLPAEVTVISSSMAEWMQVIIVESVRRASGRFRDERDISNDIKQHLDAEFGKLWQVVIVMGQYFASYSH 176
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1) HSP 1 Score: 318.546 bits (815), Expect = 1.524e-111 Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3 Query: 255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL Sbjct: 1 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 153
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1) HSP 1 Score: 114.775 bits (286), Expect = 2.071e-31 Identity = 59/160 (36.88%), Postives = 86/160 (53.75%), Query Frame = 3 Query: 255 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713 MD+F+ AFF ID+D S EI+ EL YM N+ + F+KKW+ IFD ++ G ITL+EYC VLG+ A + R ++P++VKI+QS++ + I +N S + + K + E L YG W C + GQ W+YFS S Sbjct: 1 MDNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSF 160
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1) HSP 1 Score: 112.079 bits (279), Expect = 2.228e-30 Identity = 63/158 (39.87%), Postives = 91/158 (57.59%), Query Frame = 3 Query: 258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAIS-SGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSL 713 D+FI+A+ ID D SG I+ DEL YM +N+Y+ +F+ KWL++FD DN+G ITLEE+C+ LG+E KR +L+ +S+ V IL S M + I++ A +S K + +NL L T W C + KGQ+W Y+S CSL Sbjct: 3 DAFIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGLEVSDFIDKRTALKESAD-FSLYNGVNILSSNMVQPNEGIVIQVIRKARELYPNSFKEQSTYIKQNLDLVLETTWHCIITKGQYWAYYSCEPECSL 159
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1) HSP 1 Score: 87.8113 bits (216), Expect = 3.175e-21 Identity = 47/152 (30.92%), Postives = 82/152 (53.95%), Query Frame = 3 Query: 258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHL 698 D+F+ AFF+ID ++ +I+ +L ++ NKN+Y +F+ KWL +FD + +G ITLEE+C VLG+E KR Q P++ ++L +++ + N I + + K I + Y W +I+GQ+W ++S+L Sbjct: 6 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEE--------RYQRAWVILIIRGQYWAHYSYL 149
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1) HSP 1 Score: 87.8113 bits (216), Expect = 3.246e-21 Identity = 47/152 (30.92%), Postives = 82/152 (53.95%), Query Frame = 3 Query: 258 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEY-----KRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHL 698 D+F+ AFF+ID ++ +I+ +L ++ NKN+Y +F+ KWL +FD + +G ITLEE+C VLG+E KR Q P++ ++L +++ + N I + + K I + Y W +I+GQ+W ++S+L Sbjct: 3 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEE--------RYQRAWVILIIRGQYWAHYSYL 146 The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008542 ID=SMED30008542|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=791bpback to top protein sequence of SMED30008542-orf-1 >SMED30008542-orf-1 ID=SMED30008542-orf-1|Name=SMED30008542-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=170bp MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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