SMED30008517

Overview
NameSMED30008517
Smed IDSMED30008517
Length (bp)3042
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008517 (SMED30008517) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008517 (SMED30008517) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008517 (SMED30008517) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008517 (SMED30008517) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008517

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008517SMESG000024157.1 SMESG000024156.1 SmedASXL_014108SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30008517SMESG000024157.1 SMESG000024156.1 SmedASXL_014108SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30008517 vs. Planmine SMEST
Match: SMESG000024156.1 (SMESG000024156.1)

HSP 1 Score: 720.694 bits (1859), Expect = 0.000e+0
Identity = 379/429 (88.34%), Postives = 379/429 (88.34%), Query Frame = 3
Query: 1878 MCSKKPRLESDKDQYSIEKPQEINISNLQRVTNEINEKCIRRYQEMVEFTNRNSILPTLSFQINQSKTNKTPVSNSLLKKSNEMITETPNP----------------------------------------------TERPAESKIPPTVSDTMEKSGKSSVQLENASISLDNREPLNNNRINEVFIENLFQELIHQQSPKDFLPIIRQRIIMETETQAKRFIDSFLEFFLQHKYKSFNRKLAADIAYYMILSLYSQCQQPIYDYWYSALLAAFRSQGIIPXXXXXXXXXXXXXQRSDQLIGFLVDLIRLPNVRLLSVIRWISRPADLFPAPLAATHPLVIHLIKRIVVFEYSVAMSNSEARKSFINIPLDSQRFWLDGHTTETVLQIDDAAPIVQLANQISRQYKSQNSNLKLDNLDEDIKLKYVVNK 3026
            MCSKKPRLESDKDQYSIEK QEINISNLQRVTNEINEKCIRRYQEMVEFTNRNSILPTLSFQINQSKTNKTPVSNSLLKKSNEMITETPNP                                              TERPAESKIPPTVSDTMEKSGKSSVQLENASISLDNREPLNNNRINEV IENLFQELIHQQSPKDFLPIIRQRIIMETETQAKRFIDSFLEFFLQHKYKSFNRKLAADIAYYMILSLYSQCQQPIYDYWYSALLAAFRSQGIIPVLLMANLALLVFLQRSDQLIGFLVDLIRLPNVRLLSVIRWISRPADLFPAPLAATHPLVIHLIKRIVVFEYSVAMSNSEARKSFINIPLDSQRFWLDGHTTETVLQIDD  PIVQLANQISRQYKSQNSNLKLDNLDEDIKLKYVVNK
Sbjct:    1 MCSKKPRLESDKDQYSIEKHQEINISNLQRVTNEINEKCIRRYQEMVEFTNRNSILPTLSFQINQSKTNKTPVSNSLLKKSNEMITETPNPAEKSVESFISPNLNIDNFNQSEANKNSVSNPLLKKSNEMINETPNPTERPAESKIPPTVSDTMEKSGKSSVQLENASISLDNREPLNNNRINEVLIENLFQELIHQQSPKDFLPIIRQRIIMETETQAKRFIDSFLEFFLQHKYKSFNRKLAADIAYYMILSLYSQCQQPIYDYWYSALLAAFRSQGIIPVLLMANLALLVFLQRSDQLIGFLVDLIRLPNVRLLSVIRWISRPADLFPAPLAATHPLVIHLIKRIVVFEYSVAMSNSEARKSFINIPLDSQRFWLDGHTTETVLQIDDVTPIVQLANQISRQYKSQNSNLKLDNLDEDIKLKYVVNK 429          
BLAST of SMED30008517 vs. Planmine SMEST
Match: SMESG000024157.1 (SMESG000024157.1)

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 342/349 (97.99%), Postives = 343/349 (98.28%), Query Frame = 3
Query:  441 MSRKLLEAYKQAKFTLLKHVQNNLITHMLTIEGESDLLKSIEEIIDRDKSKKISPKTVIPNETDEGESSPSYMTVDSNYDTKVNDFSGYDTDHEMDNRIGDRKKRVVLXXXXXXXXXXXXXXXXXDGNSNXXXXXXXXXXXXXXXXDSLKYRQETSVNQNNSEVQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK 1487
            MSRKLLEAYKQAKFTLLKHVQNNLITHMLTIEGESDLLKSIEEIIDRDKSKKISPK VIPNETDEGESSPSYMTVDSNYDTKVNDFSG DTDHEM NRIGDRKK+VVLSKPSKTYQ SSTKTSIKDGNSNPQETIEIIPLEEIFPEDSLKYRQETS NQNNS VQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK
Sbjct:    1 MSRKLLEAYKQAKFTLLKHVQNNLITHMLTIEGESDLLKSIEEIIDRDKSKKISPKKVIPNETDEGESSPSYMTVDSNYDTKVNDFSGDDTDHEMHNRIGDRKKQVVLSKPSKTYQNSSTKTSIKDGNSNPQETIEIIPLEEIFPEDSLKYRQETSANQNNSAVQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK 349          

HSP 2 Score: 53.9138 bits (128), Expect = 3.885e-7
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 1
Query: 1486 KIQINSTNRNILRLHRLLFRNCMTIIT 1566
            KIQINSTNRNILRLHRLLFRN MTIIT
Sbjct:  349 KIQINSTNRNILRLHRLLFRNSMTIIT 375          
BLAST of SMED30008517 vs. Planmine SMEST
Match: SMESG000024157.1 (SMESG000024157.1)

HSP 1 Score: 558.91 bits (1439), Expect = 0.000e+0
Identity = 315/322 (97.83%), Postives = 316/322 (98.14%), Query Frame = 3
Query:  522 MLTIEGESDLLKSIEEIIDRDKSKKISPKTVIPNETDEGESSPSYMTVDSNYDTKVNDFSGYDTDHEMDNRIGDRKKRVVLXXXXXXXXXXXXXXXXXDGNSNXXXXXXXXXXXXXXXXDSLKYRQETSVNQNNSEVQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK 1487
            MLTIEGESDLLKSIEEIIDRDKSKKISPK VIPNETDEGESSPSYMTVDSNYDTKVNDFSG DTDHEM NRIGDRKK+VVLSKPSKTYQ SSTKTSIKDGNSNPQETIEIIPLEEIFPEDSLKYRQETS NQNNS VQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK
Sbjct:    1 MLTIEGESDLLKSIEEIIDRDKSKKISPKKVIPNETDEGESSPSYMTVDSNYDTKVNDFSGDDTDHEMHNRIGDRKKQVVLSKPSKTYQNSSTKTSIKDGNSNPQETIEIIPLEEIFPEDSLKYRQETSANQNNSAVQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNIDKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYISTPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQSPLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIK 322          

HSP 2 Score: 53.9138 bits (128), Expect = 3.241e-7
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 1
Query: 1486 KIQINSTNRNILRLHRLLFRNCMTIIT 1566
            KIQINSTNRNILRLHRLLFRN MTIIT
Sbjct:  322 KIQINSTNRNILRLHRLLFRNSMTIIT 348          
BLAST of SMED30008517 vs. Planmine SMEST
Match: SMESG000024163.1 (SMESG000024163.1)

HSP 1 Score: 162.155 bits (409), Expect = 2.802e-40
Identity = 122/392 (31.12%), Postives = 206/392 (52.55%), Query Frame = 3
Query: 1872 REMCSKKPRLESDKDQYSIEKPQEINISNLQRVTNEINEKCIRRYQEMVEFTNRNSILPT-LSFQINQSKTNKTPVSNSLLKKSNEMITETPNPTERPAESKIPPTVSDTMEKSGKSSVQLENASISLDNREPLNNNRINEVFIEN----LFQELIHQQSPKDFLPIIRQRIIMETETQAKRFIDSFLEFFLQHKYKSFNRKLAADIAYYMILSLYSQCQQPIYDYWYSALLAAFRS----QGIIPXXXXXXXXXXXXXQRSDQLIGFLVDLIRLPNVRLLSVIRWISRPADLFPAPLAATHPLVIHLIKRIVVFEYSVAMSNSEARKSFINIPLDSQRFWLDGHTTETVLQIDDAAPIVQLANQISRQYKSQNSNLKLDNLDEDIKLKYVV 3020
            R +   KP  ES     SI K   +N+     + ++INE+ I ++Q +V FT  N +LP  L    N+SK N+  +                 P E+     I  T ++    S  S  +L++ +              NE FIEN    LF ELI Q + KD + +I QR  + TE  AK  ID     F  +K K F R    +  + +++S+ S+CQ+  Y Y+Y  LLA  +S     G++  +++A +++ + L++ D+++GF VDL+RLPN+ LL ++  ++ P + FP      +  +I L K++V++EYS   ++ E++KS  +  ++ Q+FWL+G T      + D   +++LANQI+ +YKS+N   KLD L +D+++KY +
Sbjct:  765 RNIIQSKPTEESK----SISKLLNVNLGQY-LLRHQINEEDIIKHQRLVTFTAYNPMLPEPLPVIKNKSKQNRNEI-----------------PIEKQPIKSISETTNEIKTISESSQSKLKDLN--------------NEQFIENQIVSLFHELI-QDNGKDSIGLISQRFPIATENDAKLLIDGIFNLFKSNKDKLFKRTFLINAIFQLVVSIISRCQKA-YSYYYLNLLAFIKSVETHSGLV--VILATISIFISLEQWDRIVGFFVDLLRLPNIFLLKLVSVVT-PNNFFPQQTRTKYNSIIVLFKQLVIYEYSTT-TDDESKKSLASTSIEVQKFWLNGQTVGNFQSMKDLQSVIELANQITDEYKSKNLKFKLDFLGKDLEMKYFI 1114          
The following BLAST results are available for this feature:
BLAST of SMED30008517 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008517 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000024156.10.000e+088.34SMESG000024156.1[more]
SMESG000024157.13.885e-797.99SMESG000024157.1[more]
SMESG000024157.13.241e-797.83SMESG000024157.1[more]
SMESG000024163.12.802e-4031.12SMESG000024163.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008517 ID=SMED30008517|Name=SMED30008517|organism=Schmidtea mediterranea sexual|type=transcript|length=3042bp
AAAGGGTAAAAAAAAAGAGGGTCAAAACAATCAAGATATTTTTATCGAAA
ATAAAAAATTAATTCTGTTGAATGAAGAATTGAATTGTACCCTTAATAAT
ATAAAAAAAGAATGTAAAACTTGTCAAGATCTTAAAGAACAAAACCAAGA
ATTAAAGAAAGCAAATATGATGTATCAAATGGAAATTAAGGCATTAAAAA
GAAAGGCTAGTGATCTAGATCGTGAATTAAAAGATATTAACATTTTTAAT
ACTAAATTACAAAATGAAAATATTAAATTATCGACACAAAACGATAAGTA
CAAGGAACTGAATCCCGGGAAAGTTGAAGAAATGACAAGAACAGTTGAAC
GTTTGAAGCAGTTAATTTCCAATGACACGAGACAACGGAAAATGGAAATA
AATAAAACCTGGTAATGAGTCCTATCGAGCAATCAATTCAATGAGTAGGA
AATTGCTAGAGGCATATAAGCAAGCCAAGTTTACTCTTTTAAAACATGTA
CAGAATAATCTGATAACTCACATGCTCACAATTGAAGGAGAGTCAGATTT
ATTGAAATCTATCGAGGAAATTATTGACAGAGATAAATCTAAGAAAATAT
CTCCCAAAACAGTAATTCCCAACGAAACTGACGAGGGGGAAAGCTCACCG
TCATATATGACAGTTGATTCGAATTATGATACTAAAGTTAATGACTTTTC
TGGTTATGATACTGATCACGAGATGGACAATAGAATAGGGGATAGAAAGA
AACGAGTTGTATTAAGTAAACCATCGAAAACATATCAAACTAGTTCAACA
AAAACATCAATTAAAGATGGTAATTCAAATCCACAGGAAACAATTGAAAT
TATCCCATTAGAAGAAATATTTCCGGAAGATAGTTTAAAATATCGCCAAG
AAACATCTGTAAATCAAAATAACAGTGAAGTTCAAAAAATAAGGACTTCG
TCTAAGAAAATGAGTGTAACGCCAGATGGCCCATCTTGTCGAACGCTTCA
TTACGATTCAAGTAAAAATGAAGATGAAAAAATGAATATTGATAAAGATG
TTTCTGTTGAGATGAATTCGGATAATAATGACAATAAGCAAGGAGAGGAA
TTATCTATTCCGATTAATGCTTTGAAAATTCCTGAATCAATTAACAATAT
GGATGAGTTTCCAGAAAGTTGTAGAGGTGCCGAATATATATCAACACCGT
TATGCATTGACAATGACATAGAAATAATCGGAACATACATGCAGAAACTT
AGTACCGCTACATGCATTCCGATCCATTTGATTAAAACCGAAGATTCCAA
TGAAAATAAAAATTTCTCTACAGTTTCCAAAGTTCAATCTCCTTTGAAAT
CAGATATTATCGATTGTGAGTTATCGATTAATGAAAACCCTTTAAATCTG
TCGTTTAAACCCAATCCATTCCTTACGAAAGCAGAAGTCGAAGAATTCGA
TTGTGAAATCGATTTCCCATTGAATTTATGTATAAAAAATTCAAATAAAT
TCCACCAATCGAAACATTCTGAGATTACATCGACTCCTATTCAGAAATTG
CATGACCATTATTACATAAACAATCCTCGCGAAGAATTAAATTGCAAACT
TTTCTCGTCACCATCAATCGATTTCCAAAACTTTCATAATCAAATCAATA
GTTCAATTGCAAATTCATCGATTGGAGTCTCAAAACGAGCAAATTCTGTG
GTAGAAATAGCAAAACATGATCAGACAGCTGCTGTGAAGAGATCAGGAAT
GAAGCCAATAATTGAATCCGATTTATACCAATGTGACACAATCAATAATA
ATAATAATGGGAATTTGTTAGAAAATATCGGAAAATCCATTGTAAAGCGA
AAGATAATCTATGATAAAAATAGGGAAATGTGTTCGAAAAAGCCGAGATT
GGAAAGTGATAAGGATCAATATTCCATTGAAAAACCCCAAGAAATCAATA
TCAGTAATTTACAACGGGTTACAAATGAAATTAATGAGAAATGTATTAGA
AGATATCAAGAAATGGTGGAATTTACTAACAGAAATTCTATTCTACCAAC
ATTATCATTTCAAATCAACCAATCAAAAACGAATAAAACCCCAGTTTCGA
ATTCTCTGCTTAAGAAATCCAATGAAATGATTACCGAAACTCCAAATCCA
ACAGAGAGACCAGCCGAATCAAAAATACCACCAACTGTGAGTGATACAAT
GGAAAAATCCGGAAAAAGCAGCGTCCAATTGGAAAATGCTTCGATCTCAT
TGGATAACCGAGAACCATTGAATAACAATCGAATAAATGAGGTGTTTATC
GAGAACCTATTCCAAGAATTGATTCACCAGCAAAGCCCGAAAGATTTCCT
CCCGATCATCCGACAGCGAATCATCATGGAAACCGAAACCCAGGCGAAAC
GGTTCATCGACAGTTTCCTTGAGTTCTTCCTCCAGCACAAATACAAATCA
TTCAATCGGAAGCTTGCTGCCGACATTGCATATTACATGATCCTGTCCCT
GTACAGCCAATGCCAACAGCCGATATATGATTACTGGTACAGCGCACTGC
TGGCAGCATTCAGATCACAAGGGATCATCCCGGTTCTCCTCATGGCCAAT
CTGGCGCTCCTGGTGTTCCTACAGAGATCGGATCAGTTGATCGGGTTCCT
GGTGGATCTGATCCGGCTGCCAAATGTCCGGCTACTCAGTGTTATCCGGT
GGATCAGTCGGCCTGCTGATCTGTTCCCAGCACCGCTGGCAGCCACTCAT
CCATTGGTTATCCATCTCATCAAGCGCATTGTGGTATTTGAGTACTCGGT
TGCCATGAGCAACAGTGAGGCAAGGAAATCGTTCATCAATATCCCACTGG
ATAGTCAGCGGTTTTGGCTTGACGGCCACACGACTGAGACCGTGCTGCAG
ATCGATGATGCTGCACCGATTGTACAATTGGCCAATCAGATCAGTCGCCA
ATACAAATCACAGAATTCCAATCTGAAATTGGATAATTTAGATGAAGATA
TAAAATTGAAATATGTTGTAAATAAATGATTTTCTTTTAATA
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protein sequence of SMED30008517-orf-1

>SMED30008517-orf-1 ID=SMED30008517-orf-1|Name=SMED30008517-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=138bp
KGKKKEGQNNQDIFIENKKLILLNEELNCTLNNIKKECKTCQDLKEQNQE
LKKANMMYQMEIKALKRKASDLDRELKDINIFNTKLQNENIKLSTQNDKY
KELNPGKVEEMTRTVERLKQLISNDTRQRKMEINKTW*
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protein sequence of SMED30008517-orf-2

>SMED30008517-orf-2 ID=SMED30008517-orf-2|Name=SMED30008517-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=863bp
MSRKLLEAYKQAKFTLLKHVQNNLITHMLTIEGESDLLKSIEEIIDRDKS
KKISPKTVIPNETDEGESSPSYMTVDSNYDTKVNDFSGYDTDHEMDNRIG
DRKKRVVLSKPSKTYQTSSTKTSIKDGNSNPQETIEIIPLEEIFPEDSLK
YRQETSVNQNNSEVQKIRTSSKKMSVTPDGPSCRTLHYDSSKNEDEKMNI
DKDVSVEMNSDNNDNKQGEELSIPINALKIPESINNMDEFPESCRGAEYI
STPLCIDNDIEIIGTYMQKLSTATCIPIHLIKTEDSNENKNFSTVSKVQS
PLKSDIIDCELSINENPLNLSFKPNPFLTKAEVEEFDCEIDFPLNLCIKN
SNKFHQSKHSEITSTPIQKLHDHYYINNPREELNCKLFSSPSIDFQNFHN
QINSSIANSSIGVSKRANSVVEIAKHDQTAAVKRSGMKPIIESDLYQCDT
INNNNNGNLLENIGKSIVKRKIIYDKNREMCSKKPRLESDKDQYSIEKPQ
EINISNLQRVTNEINEKCIRRYQEMVEFTNRNSILPTLSFQINQSKTNKT
PVSNSLLKKSNEMITETPNPTERPAESKIPPTVSDTMEKSGKSSVQLENA
SISLDNREPLNNNRINEVFIENLFQELIHQQSPKDFLPIIRQRIIMETET
QAKRFIDSFLEFFLQHKYKSFNRKLAADIAYYMILSLYSQCQQPIYDYWY
SALLAAFRSQGIIPVLLMANLALLVFLQRSDQLIGFLVDLIRLPNVRLLS
VIRWISRPADLFPAPLAATHPLVIHLIKRIVVFEYSVAMSNSEARKSFIN
IPLDSQRFWLDGHTTETVLQIDDAAPIVQLANQISRQYKSQNSNLKLDNL
DEDIKLKYVVNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR003123VPS9
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..156