SMED30008516

Overview
NameSMED30008516
Smed IDSMED30008516
Length (bp)970
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008516 (SMED30008516) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008516 (SMED30008516) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008516 (SMED30008516) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008516 (SMED30008516) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008516

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008516h1SMcG0004650 SmedASXL_009585SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X2 cellSMED30008516h1SMcG0004650 SmedASXL_009585SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30008516h1SMcG0004650 dd_Smed_v4_8729_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30008516h1SMcG0004650 dd_Smed_v4_8729_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008516h1SMcG0004650 dd_Smed_v6_8729_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30008516h1SMcG0004650 OX_Smed_1.0.07983ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008516 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.021972:2959..3885 +4280
Homology
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BLAST of SMED30008516 vs. TrEMBL
Match: A0A5P8I4M3 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 5.471e-166
Identity = 237/238 (99.58%), Postives = 238/238 (100.00%), Query Frame = 3 
Query:  126 MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLXXXXXXXXXXXIHQLGENKLTKARSEPDGGSWVEETKDNNQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV 839
            MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLSPYSLSSDKSSIHQLGENKLTKARSEPDGGSWVEE+KDNNQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV
Sbjct:    1 MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLSPYSLSSDKSSIHQLGENKLTKARSEPDGGSWVEESKDNNQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV 238
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BLAST of SMED30008516 vs. Planmine SMEST
Match: SMESG000066250.1 (SMESG000066250.1)

HSP 1 Score: 468.389 bits (1204), Expect = 1.118e-168
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 3 
Query:  126 MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLXXXXXXXXXXXIHQLGENKLTKARSEPDGGSWVEETKDNNQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV 839
            MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLSPYSLSSDKSSIHQLGENKLTKARSEPDGGSWVEETKD NQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV
Sbjct:    1 MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAYICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQAGSCSSGRSSLTDTSEMLSPYSLSSDKSSIHQLGENKLTKARSEPDGGSWVEETKDINQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKILANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV 238
The following BLAST results are available for this feature:
BLAST of SMED30008516 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A5P8I4M35.471e-16699.58Uncharacterized protein OS=Schmidtea mediterranea ... [more]
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BLAST of SMED30008516 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008516 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000066250.11.118e-16899.58SMESG000066250.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008516 ID=SMED30008516|Name=SMED30008516|organism=Schmidtea mediterranea sexual|type=transcript|length=970bp
CAGATTTTAGCAAATGCCATTTATATTTTTGGATTTATTTATAATTTATT
ACACAATTTAATCGAGACGTACGTCAAAATTCTAGATAAAGTTTTGGAAT
AAATTACTCCAAATACATTTCAACAATGAATCGAAACATGAAACAAATGC
CTCAGATTGTGATGGAATATGCGGTAATGATTGATGATTTTATCGCAGAA
GGTTCGACTGAACTATCTACAAAAAGAGGTGATTTTGTACAAGTACTCTA
TCATATTGACAAAAAATGGGCTTACATTTGTCGACAAGTTGATAAAAGAG
CAGGCTATGTACCTTTTCTTTATTGTGCGTTTATTAAAAGCATTACATTC
GGAAACATTGGTGATCAGTTATCAGTAGTTGCAGTGAAACCACAAACCAA
AGAGCTTCTACGAGTGAATACACAAGCCGGAAGTTGTTCCTCTGGTAGAT
CATCTTTAACGGATACATCGGAAATGCTAAGTCCATATTCATTATCCAGT
GATAAGTCATCAATTCATCAACTTGGAGAAAACAAATTAACCAAAGCTCG
TTCAGAACCCGACGGAGGGAGTTGGGTAGAGGAAACAAAGGATAATAATC
AAGAAATAATCACCCCACCAAGTCCTTATATTAATGAGCCAACTTCATCA
AAATGTGCAACTCGAAAACAGTCTGACTTTCCCGTAAATATCAAATCTTC
CAGAAGTTTCAGCGGTAAAATACTTGCAAATAAGCCTTCGTCTATGACTC
CGAATCTTGGTAAACGTGGCAGACTGTGCGAACCATACAAAACAATTGTT
AACGAACTGGAAGATTGCCTAGAAAAATTAACTCTTGTTTAAAATTTTTC
AAAAAAATAATCTTATATTTTTTTCTTTGTAAATATATCCTTTTTCGCAA
TACTTAATTTTTCGTATTTCTTGATGTTTTTTTCTCCGTGAGATTTTTTT
TTGCGTCAAAGATAATCTGC
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protein sequence of SMED30008516-orf-1

>SMED30008516-orf-1 ID=SMED30008516-orf-1|Name=SMED30008516-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MNRNMKQMPQIVMEYAVMIDDFIAEGSTELSTKRGDFVQVLYHIDKKWAY
ICRQVDKRAGYVPFLYCAFIKSITFGNIGDQLSVVAVKPQTKELLRVNTQ
AGSCSSGRSSLTDTSEMLSPYSLSSDKSSIHQLGENKLTKARSEPDGGSW
VEETKDNNQEIITPPSPYINEPTSSKCATRKQSDFPVNIKSSRSFSGKIL
ANKPSSMTPNLGKRGRLCEPYKTIVNELEDCLEKLTLV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036028SH3-like_dom_sf
IPR001452SH3_domain
IPR011511SH3_2
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001452SH3 domainSMARTSM00326SH3_2coord: 14..71
e-value: 4.3E-6
score: 36.3
IPR001452SH3 domainPFAMPF07653SH3_2coord: 15..64
e-value: 1.5E-6
score: 27.8
IPR001452SH3 domainPROSITEPS50002SH3coord: 11..72
score: 12.495
NoneNo IPR availableGENE3DG3DSA:2.30.30.40coord: 10..83
e-value: 3.6E-8
score: 34.9
NoneNo IPR availableCDDcd00174SH3coord: 15..66
e-value: 4.28447E-7
score: 43.6055
IPR036028SH3-like domain superfamilySUPERFAMILYSSF50044SH3-domaincoord: 8..83