SMED30008513
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008513 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of SMED30008513 vs. Ensembl Human
Match: APEH (acylaminoacyl-peptide hydrolase [Source:HGNC Symbol;Acc:HGNC:586]) HSP 1 Score: 383.645 bits (984), Expect = 2.634e-118 Identity = 236/730 (32.33%), Postives = 379/730 (51.92%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKF------LNNADPYIISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI G+QF + +D+GE + + PV+C+LDV+ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L Y+ T T VVVDVV + E F+G+Y L CW+ DS ++ SA + + + +D + V L + S LL ++ ++ + S+PS P L +G L SA E + V L ++ ++L PE ++ ++ +++I + S + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A E +++E D + + L GGSHGGF++ HL Y + ++ RNPVIN+ SM +TDIPDW E G + D L + M ++SP+ + ++K P+L+M+GQ+D+RVP QG+E Y+AL+ R+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVP--ENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQE--EHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWL 726
BLAST of SMED30008513 vs. Ensembl Human
Match: APEH (acylaminoacyl-peptide hydrolase [Source:HGNC Symbol;Acc:HGNC:586]) HSP 1 Score: 374.015 bits (959), Expect = 1.178e-114 Identity = 235/735 (31.97%), Postives = 380/735 (51.70%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKF------LNNADPYIISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFT-----ECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI G+QF + +D+GE + + PV+C+LDV+ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L Y+ T T VVVDVV + E F+G+Y L CW+ DS ++ SA + + + +D + V L + S LL ++ ++ + S+PS P L +G L SA E + V L ++ ++L PE ++ ++ +++I + S + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A E +++E D + + L GGSHGGF++ HL Y + ++ RNPVIN+ SM +TDIPD + + E G + D L + M ++SP+ + ++K P+L+M+GQ+D+RVP QG+E Y+AL+ R+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVP--ENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQE--EHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDCSVSCRCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWL 731
BLAST of SMED30008513 vs. Ensembl Human
Match: APEH (acylaminoacyl-peptide hydrolase [Source:HGNC Symbol;Acc:HGNC:586]) HSP 1 Score: 140.584 bits (353), Expect = 5.560e-36 Identity = 93/281 (33.10%), Postives = 145/281 (51.60%), Query Frame = -3
Query: 2146 RKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVK 2952
RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI G+QF + +D+GE + + PV+C+LDV+ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L Y+ T T VVVDVV + E F+G+Y L CW+ DS ++ SA +
Sbjct: 32 RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVP--ENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQR 308
BLAST of SMED30008513 vs. Ensembl Human
Match: APEH (acylaminoacyl-peptide hydrolase [Source:HGNC Symbol;Acc:HGNC:586]) HSP 1 Score: 123.25 bits (308), Expect = 7.517e-31 Identity = 73/229 (31.88%), Postives = 117/229 (51.09%), Query Frame = -3
Query: 2299 RKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRL 2952
RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI G+QF + +D+GE + + PV+C+LDV+ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L
Sbjct: 6 RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVP--ENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQL 230
BLAST of SMED30008513 vs. Ensembl Human
Match: APEH (acylaminoacyl-peptide hydrolase [Source:HGNC Symbol;Acc:HGNC:586]) HSP 1 Score: 85.8853 bits (211), Expect = 1.497e-17 Identity = 74/322 (22.98%), Postives = 132/322 (40.99%), Query Frame = -3
Query: 2299 MSVLKNITSVYKSYARYPNLNSAKF------LNNADPYIISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSPQ--VLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRL 3231
+S + ++Y+ +R P L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI L ++ I+ + P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L
Sbjct: 4 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIAR----------------------LKKPDQAIKAFWA---------------PGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQL 282
BLAST of SMED30008513 vs. Ensembl Celegans
Match: dpf-5 (Dipeptidyl Peptidase Four (IV) family [Source:UniProtKB/TrEMBL;Acc:Q9TYX1]) HSP 1 Score: 271.937 bits (694), Expect = 7.928e-78 Identity = 204/728 (28.02%), Postives = 334/728 (45.88%), Query Frame = -3
Query: 1123 NMSVLKNITSVYKSYARYPNLNSAKF-LNNADPYI-ISSTWTVNDLVTSK-TKNLNLNFYYEK-----DKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKS-FNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE--IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPY-NLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTD-------VVVDVVKEPIT--EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNIL-NVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFG-AAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTE-CGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3234
++ L+ + +Y A+ P + + D +I +++TW + +K T+ ++ +K D+ L+ + + E VYS+S A L + D + K + ++EL +L+ HG +H FG + ++Y+AE F ++ D K E +G +F+ + +GEQ V PVICI+D+ + LD + +S P G+ F G E LG IYC R L+YY L + ++ KI + S +FSPDG ++W + A GPH ++ E ++V +VKE + EF G W+ DS ++L+S+ + +++ ++I T N+ L N S + + +LLD+ D +L VS+P+ P + +G+L EM V + D + + EF + + Y+ I I + + PM++ HGGPH A + + G+ L VN+RGS G+G D I +L G G MDVKD A ++ + P ++ +K+ L+GGSHGGFL SHL Y KS NPV+N+ +M TDIP+W + E G + + E M+ SP++ V+ P L++IG+ D RV P +AL+AR V + YP HPL V +D +N++ WF
Sbjct: 13 DVRALEKLKDLYGDLAQIPIPTQGRIEKKSGDEFIKVTTTWDNRAMQLNKLTRQQRVSLIGKKGPEVTDRYLMTSVSVPMCNYENQRSVYSASNSRVAQLLTIGTGDDKKQYLKIYNLEEHVEEL---CADLSTQKKHGTIHGGGCMPFGCLHFSYGEGHVLYVAERNSKTSQYFDADIEWDNETKVFESKVGKKFELTESWGEQNDGVKQPVICIVDISSGNVTALDQ--IPANISPSYTKWAPGDAGVVFFGLEEQDTPRLGRIYCNNRRGNLYYYELATAKLTKISEGEISAEELQFSPDGNTLVWFQRAADGPHQAVLEMVAVEWPLKVDSAENIEKRIIVPIVKEKRSADEFQGFTFPQTATRSWSTDSKRLILSSSWCSKLELLSVNIATGNIEKLTNHSICLGTWSLLDVYDNEVLAVVSAPNRPPNVLLGQLPGTGKAEEMVWVRIDEAKAVDK--RRHLFEFSWEFVNLERDGATYEGILIVPNEGNNLPMVVNPHGGPHGASMASWPRRDLTTLLNSGYAVLQVNFRGSLGFGDDFIRALPGNCGDMDVKDVHNAVLTVLDKNP-RISRDKVVLFGGSHGGFLVSHLIGQYPGFYKSCVALNPVVNIATMHDITDIPEWCYFEGTGEYPDWTKITTTEQREKMFNSSPIAHVENATTPYLLLIGEKDLRVVPHY-RAFIRALKARGVPARVLTYPPSNHPLDEVNVEADYAINMVRWF 731
BLAST of SMED30008513 vs. Ensembl Celegans
Match: dpf-5 (Dipeptidyl Peptidase Four (IV) family [Source:UniProtKB/TrEMBL;Acc:Q9TYX1]) HSP 1 Score: 271.937 bits (694), Expect = 7.928e-78 Identity = 204/728 (28.02%), Postives = 334/728 (45.88%), Query Frame = -3
Query: 1123 NMSVLKNITSVYKSYARYPNLNSAKF-LNNADPYI-ISSTWTVNDLVTSK-TKNLNLNFYYEK-----DKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKS-FNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE--IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPY-NLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTD-------VVVDVVKEPIT--EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNIL-NVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFG-AAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTE-CGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3234
++ L+ + +Y A+ P + + D +I +++TW + +K T+ ++ +K D+ L+ + + E VYS+S A L + D + K + ++EL +L+ HG +H FG + ++Y+AE F ++ D K E +G +F+ + +GEQ V PVICI+D+ + LD + +S P G+ F G E LG IYC R L+YY L + ++ KI + S +FSPDG ++W + A GPH ++ E ++V +VKE + EF G W+ DS ++L+S+ + +++ ++I T N+ L N S + + +LLD+ D +L VS+P+ P + +G+L EM V + D + + EF + + Y+ I I + + PM++ HGGPH A + + G+ L VN+RGS G+G D I +L G G MDVKD A ++ + P ++ +K+ L+GGSHGGFL SHL Y KS NPV+N+ +M TDIP+W + E G + + E M+ SP++ V+ P L++IG+ D RV P +AL+AR V + YP HPL V +D +N++ WF
Sbjct: 13 DVRALEKLKDLYGDLAQIPIPTQGRIEKKSGDEFIKVTTTWDNRAMQLNKLTRQQRVSLIGKKGPEVTDRYLMTSVSVPMCNYENQRSVYSASNSRVAQLLTIGTGDDKKQYLKIYNLEEHVEEL---CADLSTQKKHGTIHGGGCMPFGCLHFSYGEGHVLYVAERNSKTSQYFDADIEWDNETKVFESKVGKKFELTESWGEQNDGVKQPVICIVDISSGNVTALDQ--IPANISPSYTKWAPGDAGVVFFGLEEQDTPRLGRIYCNNRRGNLYYYELATAKLTKISEGEISAEELQFSPDGNTLVWFQRAADGPHQAVLEMVAVEWPLKVDSAENIEKRIIVPIVKEKRSADEFQGFTFPQTATRSWSTDSKRLILSSSWCSKLELLSVNIATGNIEKLTNHSICLGTWSLLDVYDNEVLAVVSAPNRPPNVLLGQLPGTGKAEEMVWVRIDEAKAVDK--RRHLFEFSWEFVNLERDGATYEGILIVPNEGNNLPMVVNPHGGPHGASMASWPRRDLTTLLNSGYAVLQVNFRGSLGFGDDFIRALPGNCGDMDVKDVHNAVLTVLDKNP-RISRDKVVLFGGSHGGFLVSHLIGQYPGFYKSCVALNPVVNIATMHDITDIPEWCYFEGTGEYPDWTKITTTEQREKMFNSSPIAHVENATTPYLLLIGEKDLRVVPHY-RAFIRALKARGVPARVLTYPPSNHPLDEVNVEADYAINMVRWF 731
BLAST of SMED30008513 vs. Ensembl Celegans
Match: dpf-5 (Dipeptidyl Peptidase Four (IV) family [Source:UniProtKB/TrEMBL;Acc:Q9TYX1]) HSP 1 Score: 263.848 bits (673), Expect = 2.390e-76 Identity = 176/580 (30.34%), Postives = 275/580 (47.41%), Query Frame = -3
Query: 1123 FGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE--IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPY-NLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTD-------VVVDVVKEPIT--EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNIL-NVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFG-AAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTE-CGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 2817
FG + ++Y+AE F ++ D K E +G +F+ + +GEQ V PVICI+D+ + LD + +S P G+ F G E LG IYC R L+YY L + ++ KI + S +FSPDG ++W + A GPH ++ E ++V +VKE + EF G W+ DS ++L+S+ + +++ ++I T N+ L N S + + +LLD+ D +L VS+P+ P + +G+L EM V + D + + EF + + Y+ I I + + PM++ HGGPH A + + G+ L VN+RGS G+G D I +L G G MDVKD A ++ + P ++ +K+ L+GGSHGGFL SHL Y KS NPV+N+ +M TDIP+W + E G + + E M+ SP++ V+ P L++IG+ D RV P +AL+AR V + YP HPL V +D +N++ WF
Sbjct: 3 FGCLHFSYGEGHVLYVAERNSKTSQYFDADIEWDNETKVFESKVGKKFELTESWGEQNDGVKQPVICIVDISSGNVTALDQ--IPANISPSYTKWAPGDAGVVFFGLEEQDTPRLGRIYCNNRRGNLYYYELATAKLTKISEGEISAEELQFSPDGNTLVWFQRAADGPHQAVLEMVAVEWPLKVDSAENIEKRIIVPIVKEKRSADEFQGFTFPQTATRSWSTDSKRLILSSSWCSKLELLSVNIATGNIEKLTNHSICLGTWSLLDVYDNEVLAVVSAPNRPPNVLLGQLPGTGKAEEMVWVRIDEAKAVDK--RRHLFEFSWEFVNLERDGATYEGILIVPNEGNNLPMVVNPHGGPHGASMASWPRRDLTTLLNSGYAVLQVNFRGSLGFGDDFIRALPGNCGDMDVKDVHNAVLTVLDKNP-RISRDKVVLFGGSHGGFLVSHLIGQYPGFYKSCVALNPVVNIATMHDITDIPEWCYFEGTGEYPDWTKITTTEQREKMFNSSPIAHVENATTPYLLLIGEKDLRVVPHY-RAFIRALKARGVPARVLTYPPSNHPLDEVNVEADYAINMVRWF 576
BLAST of SMED30008513 vs. Ensembl Celegans
Match: dpf-5 (Dipeptidyl Peptidase Four (IV) family [Source:UniProtKB/TrEMBL;Acc:Q9TYX1]) HSP 1 Score: 263.848 bits (673), Expect = 2.390e-76 Identity = 176/580 (30.34%), Postives = 275/580 (47.41%), Query Frame = -3
Query: 1123 FGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE--IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPY-NLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTD-------VVVDVVKEPIT--EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNIL-NVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFG-AAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTE-CGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 2817
FG + ++Y+AE F ++ D K E +G +F+ + +GEQ V PVICI+D+ + LD + +S P G+ F G E LG IYC R L+YY L + ++ KI + S +FSPDG ++W + A GPH ++ E ++V +VKE + EF G W+ DS ++L+S+ + +++ ++I T N+ L N S + + +LLD+ D +L VS+P+ P + +G+L EM V + D + + EF + + Y+ I I + + PM++ HGGPH A + + G+ L VN+RGS G+G D I +L G G MDVKD A ++ + P ++ +K+ L+GGSHGGFL SHL Y KS NPV+N+ +M TDIP+W + E G + + E M+ SP++ V+ P L++IG+ D RV P +AL+AR V + YP HPL V +D +N++ WF
Sbjct: 3 FGCLHFSYGEGHVLYVAERNSKTSQYFDADIEWDNETKVFESKVGKKFELTESWGEQNDGVKQPVICIVDISSGNVTALDQ--IPANISPSYTKWAPGDAGVVFFGLEEQDTPRLGRIYCNNRRGNLYYYELATAKLTKISEGEISAEELQFSPDGNTLVWFQRAADGPHQAVLEMVAVEWPLKVDSAENIEKRIIVPIVKEKRSADEFQGFTFPQTATRSWSTDSKRLILSSSWCSKLELLSVNIATGNIEKLTNHSICLGTWSLLDVYDNEVLAVVSAPNRPPNVLLGQLPGTGKAEEMVWVRIDEAKAVDK--RRHLFEFSWEFVNLERDGATYEGILIVPNEGNNLPMVVNPHGGPHGASMASWPRRDLTTLLNSGYAVLQVNFRGSLGFGDDFIRALPGNCGDMDVKDVHNAVLTVLDKNP-RISRDKVVLFGGSHGGFLVSHLIGQYPGFYKSCVALNPVVNIATMHDITDIPEWCYFEGTGEYPDWTKITTTEQREKMFNSSPIAHVENATTPYLLLIGEKDLRVVPHY-RAFIRALKARGVPARVLTYPPSNHPLDEVNVEADYAINMVRWF 576
BLAST of SMED30008513 vs. Ensembl Celegans
Match: dpf-4 (Dipeptidyl Peptidase Four (IV) family [Source:UniProtKB/TrEMBL;Acc:Q19086]) HSP 1 Score: 88.5817 bits (218), Expect = 1.162e-17 Identity = 62/219 (28.31%), Postives = 103/219 (47.03%), Query Frame = -3
Query: 1180 SKKEGSCPMILML-HGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNK--EDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCH 1827
S G+ P +L+L HGGP + + + G VNYRGS G+G + L G D D + ++ ++ + KVD K+ + G S GG+L + +K+A V +L+++ T + + E L+ K E + ERSP+ +DKI+ PI + G++D VP SQ + +Y+ +RA V T L ++ + H
Sbjct: 395 SAPAGTLPPVLLLGHGGPTAPAQNNLDLKKQFFTSRGIAVFDVNYRGSTGFGTEFRRMLYKNCGVADRDDMLNGAKALVEQ--GKVDAEKVLITGSSSGGYLILSCLISPKNIIKAAVSVYGVADLLALDEDTHKLERCYNEM--------LIGKYPEQASIYEERSPIYHIDKIRTPIAFLHGREDTVVPMSQSITMYEKIRASGVTTALMLFDGEGH 603
BLAST of SMED30008513 vs. Ensembl Fly
Match: CG11319 (gene:FBgn0031835 transcript:FBtr0343159) HSP 1 Score: 53.5286 bits (127), Expect = 1.248e-6 Identity = 53/220 (24.09%), Postives = 95/220 (43.18%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELG---FHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLS-LVDKIK-CPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P IL ++ GP S TD G + + ++ RGS G G + + +G ++V D ++ SE +R+ +D ++ ++G S+GG+ A+ A + +PV N W + ++ N D +E S LS VD ++ L++ G D V Q + L ++L ++ V + IYP + H L+ V+
Sbjct: 693 PTILHVYSGPGSQLVTDHWHVDWNTYLSGSKDYIVVEIDGRGSAGQGYQLLHEVYKRLGSVEVSDQLEVSE-YLRDNLHFIDSRRMGVWGWSYGGYTAALALAGQQSIFQCGISVSPVTN------------WKLYDSTYAERYLSFPNVTDNYKGYEESDLSKYVDNLRDRQFLLVHGTADDNVHVQQSMVLARSLTSKGVLYKQQIYPDEGHSLSGVK 899
BLAST of SMED30008513 vs. Ensembl Fly
Match: CG11319 (gene:FBgn0031835 transcript:FBtr0343160) HSP 1 Score: 53.1434 bits (126), Expect = 1.440e-6 Identity = 53/220 (24.09%), Postives = 95/220 (43.18%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELG---FHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLS-LVDKIK-CPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P IL ++ GP S TD G + + ++ RGS G G + + +G ++V D ++ SE +R+ +D ++ ++G S+GG+ A+ A + +PV N W + ++ N D +E S LS VD ++ L++ G D V Q + L ++L ++ V + IYP + H L+ V+
Sbjct: 693 PTILHVYSGPGSQLVTDHWHVDWNTYLSGSKDYIVVEIDGRGSAGQGYQLLHEVYKRLGSVEVSDQLEVSE-YLRDNLHFIDSRRMGVWGWSYGGYTAALALAGQQSIFQCGISVSPVTN------------WKLYDSTYAERYLSFPNVTDNYKGYEESDLSKYVDNLRDRQFLLVHGTADDNVHVQQSMVLARSLTSKGVLYKQQIYPDEGHSLSGVK 899
BLAST of SMED30008513 vs. Ensembl Fly
Match: CG11319 (gene:FBgn0031835 transcript:FBtr0089641) HSP 1 Score: 53.1434 bits (126), Expect = 1.440e-6 Identity = 53/220 (24.09%), Postives = 95/220 (43.18%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELG---FHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLS-LVDKIK-CPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P IL ++ GP S TD G + + ++ RGS G G + + +G ++V D ++ SE +R+ +D ++ ++G S+GG+ A+ A + +PV N W + ++ N D +E S LS VD ++ L++ G D V Q + L ++L ++ V + IYP + H L+ V+
Sbjct: 693 PTILHVYSGPGSQLVTDHWHVDWNTYLSGSKDYIVVEIDGRGSAGQGYQLLHEVYKRLGSVEVSDQLEVSE-YLRDNLHFIDSRRMGVWGWSYGGYTAALALAGQQSIFQCGISVSPVTN------------WKLYDSTYAERYLSFPNVTDNYKGYEESDLSKYVDNLRDRQFLLVHGTADDNVHVQQSMVLARSLTSKGVLYKQQIYPDEGHSLSGVK 899
BLAST of SMED30008513 vs. Ensembl Zebrafish
Match: apeh (acylaminoacyl-peptide hydrolase [Source:ZFIN;Acc:ZDB-GENE-030131-9870]) HSP 1 Score: 383.259 bits (983), Expect = 4.052e-118 Identity = 240/733 (32.74%), Postives = 380/733 (51.84%), Query Frame = -3
Query: 1117 ITSVYKSYARYPNLNSAKFLNNADPYIIS----------STWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSPQVLMDE--EIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQN-------------VSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDH--IPEFDHPKFGKKKYQSIFISSK---KEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3213
I +Y+ R+P+L A + P I S + WT DL + +N+ Y DK+++ P E ++ V S S + +A+ E N + G+E + + +W +K +NL ++ HG V++ FG W L+YIAE K+PK S+FQ + D+ + + G+QF+Y +D+GE L + +PV+C+LD++ I VL+ +S G P GL F G+ + P+ LGL C R LFY L S + ++ ++ S SP+ SPD I++LE+ S H+QC RL ++ T T VVVDVV+ + FTG+Y+ L+ CW+ DS +L SA + + ++ ++D ++ + L S LL+++ ++V SSP++ P L +G L E+++ V L ++ + Q L+ PE D+ ++ + ++ + K +G P+I+M HGGPHS +++ + A + ++GF L+VNYRGS G+GQD++ SL G G DVKD A + ++++ D K+ + GGSHGGFLA HL Y K+ RNP NL SM TDIPDW E G +K D L + M +SP+ V K+K P+L+M+G+ DKRVP QG+E YKAL++ V + YP + H L+ V+A SD F+N W +
Sbjct: 28 IAQLYRDQCRFPSLCRA----DVGPVITSGYGGQYSNIYTEWTQRDL--ERMENVKFCRQYIVFHHDKSVVYSGPSGNCTEIRGELLSVDSPSGEMKAVLRE---NTTEGEEKQ--FLEIWHKNRKLKCLNLTTLNKHGKVYEDD-QFGCLVWSHSETHLLYIAEKKRPKTESYFQQGPETSLTPDEEEPIKTDKKEETVP-GHQFEYYEDWGENLVSKSSPVLCVLDIEGSNISVLEGIPA--AISPGQAFWAPADTGLLFVGWWHEPFRLGLKSCGNRRSSLFYVDLASGKCDQLSSDSISVCSPRLSPDHCRIVYLESGVSCSHLQCHRLCVFDWYTKQTSVVVDVVQRARDDGFTGIYSFLLSPRCWSADSERVLFCSAQRSRKELFVVDTSSREITRLTSQSQEGSWNLLNIHRDLMVVSCSSPNSPPDLRVGFLPGKGSESKISWVTLEESQPQSDI-SWQTLNFSPPPEQDNTQYPGLDFDALLLKPKDTPADGKLPLIVMPHGGPHSVLVSEWILSTAVLCKMGFSVLLVNYRGSLGFGQDNVFSLPGNCGTQDVKDVQFAVDSVLKQ--GGFDEQKVAVIGGSHGGFLACHLIGQYPGFYKACVARNPT-NLASMVCCTDIPDWCIVEAGFDYKPDIQLEPAILEQMLIKSPIKHVAKVKTPVLLMLGEGDKRVPNKQGIEYYKALKSLQVPVRVLWYPGNNHSLSKVDAESDGFMNGALWMIQ 741
BLAST of SMED30008513 vs. Ensembl Zebrafish
Match: zgc:136971 (zgc:136971 [Source:ZFIN;Acc:ZDB-GENE-060421-3674]) HSP 1 Score: 343.199 bits (879), Expect = 1.373e-103 Identity = 199/617 (32.25%), Postives = 330/617 (53.48%), Query Frame = -3
Query: 1117 ESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKK--PKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVS-------ETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 2925
E+ + +W KS++L ++ HG V++ + FG W +KL+Y+AE K+ P S F + + D +E ++ Y +D+GE L +PV+C+ D+++ + + P +S PNG G+ F G N P+ LGL +C R L+Y + + + + S SP+ SPD +I++L+ + GPH QC +M Y++ + V+VDV++ +F G+Y L +CW+ DS + +SA C+NK + + I+ V + S LL + +++ SSP+ P L G L +M+L L ++ +L P + ++ +F + +I + S + P+++ +HGGPH+ F D+ AA+ +LGF LMVNYRGS G+GQDSI+SLLG +G DVKD +A ++ + +D +++ + GGSHGGFLA HL Y D ++ A RNPVIN ++ +DI DW ++ G+++ FD L + + +M ++SP+ +I+ P+L+M+G D+RV P QGLELY+AL++R+ L + + H L+ V SD F+N+L WF E
Sbjct: 103 EAGHQYLEVWGQNGLEKSLDLTALNKHGRVYEDA-QFGCLAWSPCEKKLLYVAEKKRVEPSVHSSFGSANEDGGLVLLEEQDKNVYVEDWGEGLVGKSSPVLCVADLNKATVIIYAGVP--PHISPAQALWAPNGRGVVFVGLWNEPFRLGLKFCSNRRSALYYLDMKGN-CECLSSDGMSVSSPRMSPDSCWIVYLQGEVFGPHHQCLSMMLYDMKNRSSSVLVDVIRRAEKGQFAGIYE-SLPSHCWSADSERVFFSSA--CENKKAVFSVDRTTGKIIQVCGLDVLRLDDFGSVQLLTIQKDLMVLSCSSPNQPPCLRAGFLSKVNRAEDMQLSNLGGADVYEGFDWQPMLITPPSQEENHQFSGLNFGAILLKPYSPPERRKTPLVVNIHGGPHAHFAADWNATAAALTKLGFAVLMVNYRGSTGFGQDSIESLLGNVGSQDVKDVQRAVLCALQN-ETTLDPDRVVVMGGSHGGFLACHLIGQYPDFYRACAARNPVINAATLLGTSDIVDWRYSSVGLQYAFDRLPTSQSLISMLDKSPIIHAPQIRAPVLLMLGGRDRRVSPHQGLELYRALKSRNTPVRLLWFSDEGHTLSKVNTQSDCFLNILLWFKE 711
BLAST of SMED30008513 vs. Ensembl Zebrafish
Match: zgc:136971 (zgc:136971 [Source:ZFIN;Acc:ZDB-GENE-060421-3674]) HSP 1 Score: 343.199 bits (879), Expect = 1.373e-103 Identity = 199/617 (32.25%), Postives = 330/617 (53.48%), Query Frame = -3
Query: 1117 ESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKK--PKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVS-------ETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 2925
E+ + +W KS++L ++ HG V++ + FG W +KL+Y+AE K+ P S F + + D +E ++ Y +D+GE L +PV+C+ D+++ + + P +S PNG G+ F G N P+ LGL +C R L+Y + + + + S SP+ SPD +I++L+ + GPH QC +M Y++ + V+VDV++ +F G+Y L +CW+ DS + +SA C+NK + + I+ V + S LL + +++ SSP+ P L G L +M+L L ++ +L P + ++ +F + +I + S + P+++ +HGGPH+ F D+ AA+ +LGF LMVNYRGS G+GQDSI+SLLG +G DVKD +A ++ + +D +++ + GGSHGGFLA HL Y D ++ A RNPVIN ++ +DI DW ++ G+++ FD L + + +M ++SP+ +I+ P+L+M+G D+RV P QGLELY+AL++R+ L + + H L+ V SD F+N+L WF E
Sbjct: 103 EAGHQYLEVWGQNGLEKSLDLTALNKHGRVYEDA-QFGCLAWSPCEKKLLYVAEKKRVEPSVHSSFGSANEDGGLVLLEEQDKNVYVEDWGEGLVGKSSPVLCVADLNKATVIIYAGVP--PHISPAQALWAPNGRGVVFVGLWNEPFRLGLKFCSNRRSALYYLDMKGN-CECLSSDGMSVSSPRMSPDSCWIVYLQGEVFGPHHQCLSMMLYDMKNRSSSVLVDVIRRAEKGQFAGIYE-SLPSHCWSADSERVFFSSA--CENKKAVFSVDRTTGKIIQVCGLDVLRLDDFGSVQLLTIQKDLMVLSCSSPNQPPCLRAGFLSKVNRAEDMQLSNLGGADVYEGFDWQPMLITPPSQEENHQFSGLNFGAILLKPYSPPERRKTPLVVNIHGGPHAHFAADWNATAAALTKLGFAVLMVNYRGSTGFGQDSIESLLGNVGSQDVKDVQRAVLCALQN-ETTLDPDRVVVMGGSHGGFLACHLIGQYPDFYRACAARNPVINAATLLGTSDIVDWRYSSVGLQYAFDRLPTSQSLISMLDKSPIIHAPQIRAPVLLMLGGRDRRVSPHQGLELYRALKSRNTPVRLLWFSDEGHTLSKVNTQSDCFLNILLWFKE 711
BLAST of SMED30008513 vs. Ensembl Zebrafish
Match: zgc:136971 (zgc:136971 [Source:ZFIN;Acc:ZDB-GENE-060421-3674]) HSP 1 Score: 313.538 bits (802), Expect = 9.205e-93 Identity = 192/619 (31.02%), Postives = 322/619 (52.02%), Query Frame = -3
Query: 1117 ESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKK--PKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSK-EIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVS-------ETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHG-GPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 2925
E+ + +W KS++L ++ HG V++ + FG W +KL+Y+AE K+ P S F + + D +E ++ Y +D+GE L +PV+C+ D+++ + + P +S PNG G+ F G N P+ LGL +C R L+Y LD K + + + S SP+ SPD +I++L+ + GPH QC +M Y++ + V+VDV++ +F G+Y L +CW+ DS + +SA C+NK + + I+ V + S LL + +++ SSP+ P L G L +M+L L ++ +L P + ++ +F + +I + S + P+++ +H HS+ + A+ + VNYRGS G+GQDSI+SLLG +G DVKD +A ++ + +D +++ + GGSHGGFLA HL Y D ++ A RNPVIN ++ +DI DW ++ G+++ FD L + + +M ++SP+ +I+ P+L+M+G D+RV P QGLELY+AL++R+ L + + H L+ V SD F+N+L WF E
Sbjct: 103 EAGHQYLEVWGQNGLEKSLDLTALNKHGRVYEDA-QFGCLAWSPCEKKLLYVAEKKRVEPSVHSSFGSANEDGGLVLLEEQDKNVYVEDWGEGLVGKSSPVLCVADLNKATVIIYAGVP--PHISPAQALWAPNGRGVVFVGLWNEPFRLGLKFCSNRRSALYY--LDMKGNCECLSSDGMSVSSPRMSPDSCWIVYLQGEVFGPHHQCLSMMLYDMKNRSSSVLVDVIRRAEKGQFAGIYE-SLPSHCWSADSERVFFSSA--CENKKAVFSVDRTTGKIIQVCGLDVLRLDDFGSVQLLTIQKDLMVLSCSSPNQPPCLRAGFLSKVNRAEDMQLSNLGGADVYEGFDWQPMLITPPSQEENHQFSGLNFGAILLKPYSPPERRKTPLVVNIHVFKCHSSSSDNIKSRKRALVYSVILYIAVNYRGSTGFGQDSIESLLGNVGSQDVKDVQRAVLCALQN-ETTLDPDRVVVMGGSHGGFLACHLIGQYPDFYRACAARNPVINAATLLGTSDIVDWRYSSVGLQYAFDRLPTSQSLISMLDKSPIIHAPQIRAPVLLMLGGRDRRVSPHQGLELYRALKSRNTPVRLLWFSDEGHTLSKVNTQSDCFLNILLWFKE 712
BLAST of SMED30008513 vs. Ensembl Xenopus
Match: APEH (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:100491896]) HSP 1 Score: 411.379 bits (1056), Expect = 7.950e-129 Identity = 246/723 (34.02%), Postives = 379/723 (52.42%), Query Frame = -3
Query: 1126 KNITSVYKSYARYPNLNSAKFLNNADPYI------ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE----------IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPIT-EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETV-NSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWW 3219
K+I ++Y+ ++++P+L+S I + W+ DL S+ + D N ++ S EI ++ S S +A+ E K+ K T + +W +KSINL +D HG V++ FG W L+YIAE K+PK SFFQ + + S E G+QF +D+GE L N P +C+LD++ I VL+ + +S G P+ G+ F G+ + P+ +GL YC R LF+ L + + + ++ + SP+ SPD I++L+ + GPH QC +L+ Y+ T T VVVD V I +F+G+Y L CW+ DS ++L++A Q + ++D T NV L E+ S L+ ++ L+V SSPS P L +G L S+ E E+ + L ++ H P E ++PK+ ++SI + K E P+++ HGGPHS F +++ +F A + ++GF +VNYRGS G+GQDSI SL G IG DVKD A E +++E P +D +KI L GGSHGGFL+ HL Y + RNPV N+ +M +TDIPDW E G+ + + L + M ++SP+ V ++K P+L+M+G++D+RVP QGLE Y+AL+A V L YP + H LA V+A SD F+N+ W
Sbjct: 6 KDIAALYREFSQFPSLSSVSIGPEVTTQYGGKYSNIYTEWSQRDLDRSEQVKFCRQYIVFHDNNTVVYSGACGNGSEIKGELLSKESPSGTLKAVLRETKNI----KGEDTQFLEIWERNRKVKSINLTALDKHGKVYEDE-QFGCLSWSHSETHLLYIAERKRPKAESFFQAATGELSTGASEEEEDGINKPIKGDQFVLHEDWGEGLVNKSVPALCVLDIEGSNISVLEG--IPDHISPGQAFWSPDDTGVVFVGWWHSPFRVGLKYCANRRSALFFVDLTGGKCEMLSSDSSAVFSPRLSPDKCRIVYLKCNVYGPHQQCCQLVMYDWYTKVTSVVVDTVPRSIEGKFSGIYTSLLAPFCWSADSQRVILSTAQHSQENLFVVDTMTGNVTHLFSKESSPGSWKLMAIDRDLLVVSFSSPSCPPTLKVGFLPSSGREEEITWISLEEESPIVDIEWSFKTHHPPQEQENPKYPGLVFESILLKPKNSPPESKIPLVVYPHGGPHSTFVSEWMLFPAVLCKMGFAVQLVNYRGSLGFGQDSILSLPGNIGDQDVKDVQFAVEQVLKEDP--IDPHKIVLCGGSHGGFLSCHLIGQYPGFYVACIARNPVTNVPAMVGSTDIPDWCLVESGLPYSSETLPDPAQWGDMLKKSPIGYVSQVKTPVLLMLGEEDRRVPNKQGLEYYRALKAHGVPARLLWYPGNNHSLAKVDAESDGFMNIALW 719
BLAST of SMED30008513 vs. Ensembl Xenopus
Match: APEH (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:100491896]) HSP 1 Score: 410.994 bits (1055), Expect = 2.127e-128 Identity = 246/723 (34.02%), Postives = 379/723 (52.42%), Query Frame = -3
Query: 1126 KNITSVYKSYARYPNLNSAKFLNNADPYI------ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE----------IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPIT-EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETV-NSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWW 3219
K+I ++Y+ ++++P+L+S I + W+ DL S+ + D N ++ S EI ++ S S +A+ E K+ K T + +W +KSINL +D HG V++ FG W L+YIAE K+PK SFFQ + + S E G+QF +D+GE L N P +C+LD++ I VL+ + +S G P+ G+ F G+ + P+ +GL YC R LF+ L + + + ++ + SP+ SPD I++L+ + GPH QC +L+ Y+ T T VVVD V I +F+G+Y L CW+ DS ++L++A Q + ++D T NV L E+ S L+ ++ L+V SSPS P L +G L S+ E E+ + L ++ H P E ++PK+ ++SI + K E P+++ HGGPHS F +++ +F A + ++GF +VNYRGS G+GQDSI SL G IG DVKD A E +++E P +D +KI L GGSHGGFL+ HL Y + RNPV N+ +M +TDIPDW E G+ + + L + M ++SP+ V ++K P+L+M+G++D+RVP QGLE Y+AL+A V L YP + H LA V+A SD F+N+ W
Sbjct: 25 KDIAALYREFSQFPSLSSVSIGPEVTTQYGGKYSNIYTEWSQRDLDRSEQVKFCRQYIVFHDNNTVVYSGACGNGSEIKGELLSKESPSGTLKAVLRETKNI----KGEDTQFLEIWERNRKVKSINLTALDKHGKVYEDE-QFGCLSWSHSETHLLYIAERKRPKAESFFQAATGELSTGASEEEEDGINKPIKGDQFVLHEDWGEGLVNKSVPALCVLDIEGSNISVLEG--IPDHISPGQAFWSPDDTGVVFVGWWHSPFRVGLKYCANRRSALFFVDLTGGKCEMLSSDSSAVFSPRLSPDKCRIVYLKCNVYGPHQQCCQLVMYDWYTKVTSVVVDTVPRSIEGKFSGIYTSLLAPFCWSADSQRVILSTAQHSQENLFVVDTMTGNVTHLFSKESSPGSWKLMAIDRDLLVVSFSSPSCPPTLKVGFLPSSGREEEITWISLEEESPIVDIEWSFKTHHPPQEQENPKYPGLVFESILLKPKNSPPESKIPLVVYPHGGPHSTFVSEWMLFPAVLCKMGFAVQLVNYRGSLGFGQDSILSLPGNIGDQDVKDVQFAVEQVLKEDP--IDPHKIVLCGGSHGGFLSCHLIGQYPGFYVACIARNPVTNVPAMVGSTDIPDWCLVESGLPYSSETLPDPAQWGDMLKKSPIGYVSQVKTPVLLMLGEEDRRVPNKQGLEYYRALKAHGVPARLLWYPGNNHSLAKVDAESDGFMNIALW 738
BLAST of SMED30008513 vs. Ensembl Xenopus
Match: APEH (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:100491896]) HSP 1 Score: 407.527 bits (1046), Expect = 2.605e-127 Identity = 245/727 (33.70%), Postives = 378/727 (51.99%), Query Frame = -3
Query: 1126 KNITSVYKSYARYPNLNSAKFLNNADPYI------ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE---------------IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPIT-EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWW 3219
K+I ++Y+ ++++P+L+S I + W+ DL S+ + D N ++ S EI ++ S S +A+ E K+ K T + +W +KSINL +D HG V++ FG W L+YIAE K+PK SFFQ + + S E G+QF +D+GE L N P +C+LD++ I VL+ + +S G P+ G+ F G+ + P+ +GL YC R LF+ L + + + ++ + SP+ SPD I++L+ + GPH QC +L+ Y+ T T VVVD V I +F+G+Y L CW+ DS ++L++A Q + ++D T NV L + S L+ ++ L+V SSPS P L +G L S+ E E+ + L ++ H P E ++PK+ ++SI + K E P+++ HGGPHS F +++ +F A + ++GF +VNYRGS G+GQDSI SL G IG DVKD A E +++E P +D +KI L GGSHGGFL+ HL Y + RNPV N+ +M +TDIPDW E G+ + + L + M ++SP+ V ++K P+L+M+G++D+RVP QGLE Y+AL+A V L YP + H LA V+A SD F+N+ W
Sbjct: 8 KDIAALYREFSQFPSLSSVSIGPEVTTQYGGKYSNIYTEWSQRDLDRSEQVKFCRQYIVFHDNNTVVYSGACGNGSEIKGELLSKESPSGTLKAVLRETKNI----KGEDTQFLEIWERNRKVKSINLTALDKHGKVYEDE-QFGCLSWSHSETHLLYIAERKRPKAESFFQAATGELSTGASEEEEDGINKPIKVRDGSGDQFVLHEDWGEGLVNKSVPALCVLDIEGSNISVLEG--IPDHISPGQAFWSPDDTGVVFVGWWHSPFRVGLKYCANRRSALFFVDLTGGKCEMLSSDSSAVFSPRLSPDKCRIVYLKCNVYGPHQQCCQLVMYDWYTKVTSVVVDTVPRSIEGKFSGIYTSLLAPFCWSADSQRVILSTAQHSQENLFVVDTMTGNVTHLF---SKGSWKLMAIDRDLLVVSFSSPSCPPTLKVGFLPSSGREEEITWISLEEESPIVDIEWSFKTHHPPQEQENPKYPGLVFESILLKPKNSPPESKIPLVVYPHGGPHSTFVSEWMLFPAVLCKMGFAVQLVNYRGSLGFGQDSILSLPGNIGDQDVKDVQFAVEQVLKEDP--IDPHKIVLCGGSHGGFLSCHLIGQYPGFYVACIARNPVTNVPAMVGSTDIPDWCLVESGLPYSSETLPDPAQWGDMLKKSPIGYVSQVKTPVLLMLGEEDRRVPNKQGLEYYRALKAHGVPARLLWYPGNNHSLAKVDAESDGFMNIALW 722
BLAST of SMED30008513 vs. Ensembl Xenopus
Match: APEH (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:100491896]) HSP 1 Score: 387.111 bits (993), Expect = 8.430e-120 Identity = 236/721 (32.73%), Postives = 366/721 (50.76%), Query Frame = -3
Query: 1126 KNITSVYKSYARYPNLNSAKFLNNADPYI------ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE----------IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWW 3219
K+I ++Y+ ++++P+L+S I + W+ DL S+ + D N ++ S EI ++ S S +A+ E K+ K T + +W +KSINL +D HG V++ FG W L+YIAE K+PK SFFQ + + S E G+QF +D+GE L N P +C+LD++ I VL+ + +S G P+ G+ F G+ + P+ +GL YC R LF+ L + + + ++ + SP+ SPD I++L+ + GPH QC K +F+G+Y L CW+ DS ++L++A Q + ++D T NV L + S L+ ++ L+V SSPS P L +G L S+ E E+ + L ++ H P E ++PK+ ++SI + K E P+++ HGGPHS F +++ +F A + ++GF +VNYRGS G+GQDSI SL G IG DVKD A E +++E P +D +KI L GGSHGGFL+ HL Y + RNPV N+ +M +TDIPDW E G+ + + L + M ++SP+ V ++K P+L+M+G++D+RVP QGLE Y+AL+A V L YP + H LA V+A SD F+N+ W
Sbjct: 8 KDIAALYREFSQFPSLSSVSIGPEVTTQYGGKYSNIYTEWSQRDLDRSEQVKFCRQYIVFHDNNTVVYSGACGNGSEIKGELLSKESPSGTLKAVLRETKNI----KGEDTQFLEIWERNRKVKSINLTALDKHGKVYEDE-QFGCLSWSHSETHLLYIAERKRPKAESFFQAATGELSTGASEEEEDGINKPIKGDQFVLHEDWGEGLVNKSVPALCVLDIEGSNISVLEG--IPDHISPGQAFWSPDDTGVVFVGWWHSPFRVGLKYCANRRSALFFVDLTGGKCEMLSSDSSAVFSPRLSPDKCRIVYLKCNVYGPHQQCCH---------------SYTKLQCRKFSGIYTSLLAPFCWSADSQRVILSTAQHSQENLFVVDTMTGNVTHLF---SKGSWKLMAIDRDLLVVSFSSPSCPPTLKVGFLPSSGREEEITWISLEEESPIVDIEWSFKTHHPPQEQENPKYPGLVFESILLKPKNSPPESKIPLVVYPHGGPHSTFVSEWMLFPAVLCKMGFAVQLVNYRGSLGFGQDSILSLPGNIGDQDVKDVQFAVEQVLKEDP--IDPHKIVLCGGSHGGFLSCHLIGQYPGFYVACIARNPVTNVPAMVGSTDIPDWCLVESGLPYSSETLPDPAQWGDMLKKSPIGYVSQVKTPVLLMLGEEDRRVPNKQGLEYYRALKAHGVPARLLWYPGNNHSLAKVDAESDGFMNIALW 701
BLAST of SMED30008513 vs. Ensembl Xenopus
Match: APEH (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:100491896]) HSP 1 Score: 376.711 bits (966), Expect = 4.312e-116 Identity = 230/687 (33.48%), Postives = 354/687 (51.53%), Query Frame = -3
Query: 1126 ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIE----------IGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPIT-EFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP-EFDHPKFGKKKYQSIFISSK---KEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWW 3132
I + W+ DL S+ + D N ++ S EI ++ S S +A+ E K+ K T + +W +KSINL +D HG V++ FG W L+YIAE K+PK SFFQ + + S E G+QF +D+GE L N P +C+LD++ I VL+ + +S G P+ G+ F G+ + P+ +GL YC R LF+ L + + + ++ + SP+ SPD I++L+ + GPH QC +L+ Y+ T T VVVD V I +F+G+Y L CW+ DS ++L++A Q + ++D T N + S L+ ++ L+V SSPS P L +G L S+ E E+ + L ++ H P E ++PK+ ++SI + K E P+++ HGGPHS F +++ +F A + ++GF +VNYRGS G+GQDSI SL C+ E +++E P +D +KI L GGSHGGFL+ HL Y + RNPV N+ +M +TDIPDW E G+ + + L + M ++SP+ V ++K P+L+M+G++D+RVP QGLE Y+AL+A V L YP + H LA V+A SD F+N+ W
Sbjct: 32 IYTEWSQRDLDRSEQVKFCRQYIVFHDNNTVVYSGACGNGSEIKGELLSKESPSGTLKAVLRETKNI----KGEDTQFLEIWERNRKVKSINLTALDKHGKVYEDE-QFGCLSWSHSETHLLYIAERKRPKAESFFQAATGELSTGASEEEEDGINKPIKGDQFVLHEDWGEGLVNKSVPALCVLDIEGSNISVLEG--IPDHISPGQAFWSPDDTGVVFVGWWHSPFRVGLKYCANRRSALFFVDLTGGKCEMLSSDSSAVFSPRLSPDKCRIVYLKCNVYGPHQQCCQLVMYDWYTKVTSVVVDTVPRSIEGKFSGIYTSLLAPFCWSADSQRVILSTAQHSQENLFVVDTMTGN-------SSPGSWKLMAIDRDLLVVSFSSPSCPPTLKVGFLPSSGREEEITWISLEEESPIVDIEWSFKTHHPPQEQENPKYPGLVFESILLKPKNSPPESKIPLVVYPHGGPHSTFVSEWMLFPAVLCKMGFAVQLVNYRGSLGFGQDSILSLPD-----TYTLCL---EQVLKEDP--IDPHKIVLCGGSHGGFLSCHLIGQYPGFYVACIARNPVTNVPAMVGSTDIPDWCLVESGLPYSSETLPDPAQWGDMLKKSPIGYVSQVKTPVLLMLGEEDRRVPNKQGLEYYRALKAHGVPARLLWYPGNNHSLAKVDAESDGFMNIALW 694
BLAST of SMED30008513 vs. Ensembl Mouse
Match: Apeh (acylpeptide hydrolase [Source:MGI Symbol;Acc:MGI:88041]) HSP 1 Score: 406.371 bits (1043), Expect = 6.262e-127 Identity = 233/730 (31.92%), Postives = 382/730 (52.33%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P+L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA E+ G+QF + +D+GE + + PV+C+LD++ I VL+ +S G P G+ F G+ + P+ LG+ YC R L+Y L + + + DE+ + SP+ SPD +++L+ + PH QC +L Y+ T T +VVD+V + E F+G+Y L CW+ DS ++ S + + + +D +T +V L + S LL ++ ++ + S+P+ P L +G L A E + V L ++ ++L P+ ++ ++ +++I + +S + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A + +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN++SM TDIPDW E G + DYL + + M ++SP+ + ++K P+L+M+GQ+D+RVP QGLE Y AL+AR+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPQEAAALYRGLSRQPSLSAACLGPEVTTQYGGLYRTVHTEWTQRDL--DRMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGAVSGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIESGNISVLEGVP--ENVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCELLSDESLAVCSPRLSPDQCRVVYLQYPSLAPHHQCSQLFLYDWYTKVTSLVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSVQRSRQDLFAVDTQTGSVTSLTAGGSAGSWKLLTIDRDLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEPIPDIHWGIRVLHPPPDQENVQYADLDFEAILLQPSNSPDKSQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVQQVLQE--EHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDIPDWCMVETGFPYSNDYLPDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQGLEYYHALKARNVPVRLLLYPKSTHALSEVEVESDSFMNTVLWL 726
BLAST of SMED30008513 vs. Ensembl Mouse
Match: Apeh (acylpeptide hydrolase [Source:MGI Symbol;Acc:MGI:88041]) HSP 1 Score: 377.867 bits (969), Expect = 1.765e-116 Identity = 225/730 (30.82%), Postives = 371/730 (50.82%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P+L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA E+ G+QF + +D+GE + + PV+C+LD++ I VL+ +S G P G+ F G+ + P+ LG+ YC R L+Y L + + + DE+ + SP+ SPD +++L+ + PH QC +L Y+ T T +VVD+V + E F+G+Y L CW+ DS ++ S + + + +D +T +V L + S LL ++ ++ + S+P+ P L +G L A E + V L ++ ++L P+ ++ ++ +++I + +S + PM++M H A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A + +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN++SM TDIPDW E G + DYL + + M ++SP+ + ++K P+L+M+GQ+D+RVP QGLE Y AL+AR+V L +YPK H L+ VE SDSF+N + W
Sbjct: 6 LSEPQEAAALYRGLSRQPSLSAACLGPEVTTQYGGLYRTVHTEWTQRDL--DRMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGAVSGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIESGNISVLEGVP--ENVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCELLSDESLAVCSPRLSPDQCRVVYLQYPSLAPHHQCSQLFLYDWYTKVTSLVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSVQRSRQDLFAVDTQTGSVTSLTAGGSAGSWKLLTIDRDLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEPIPDIHWGIRVLHPPPDQENVQYADLDFEAILLQPSNSPDKSQVPMVVMPH---------------AMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVQQVLQE--EHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDIPDWCMVETGFPYSNDYLPDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQGLEYYHALKARNVPVRLLLYPKSTHALSEVEVESDSFMNTVLWL 710
BLAST of SMED30008513 vs. UniProt/SwissProt
Match: sp|Q8R146|APEH_MOUSE (Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3) HSP 1 Score: 406.371 bits (1043), Expect = 4.380e-126 Identity = 233/730 (31.92%), Postives = 382/730 (52.33%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P+L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA E+ G+QF + +D+GE + + PV+C+LD++ I VL+ +S G P G+ F G+ + P+ LG+ YC R L+Y L + + + DE+ + SP+ SPD +++L+ + PH QC +L Y+ T T +VVD+V + E F+G+Y L CW+ DS ++ S + + + +D +T +V L + S LL ++ ++ + S+P+ P L +G L A E + V L ++ ++L P+ ++ ++ +++I + +S + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A + +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN++SM TDIPDW E G + DYL + + M ++SP+ + ++K P+L+M+GQ+D+RVP QGLE Y AL+AR+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPQEAAALYRGLSRQPSLSAACLGPEVTTQYGGLYRTVHTEWTQRDL--DRMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGAVSGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIESGNISVLEGVP--ENVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCELLSDESLAVCSPRLSPDQCRVVYLQYPSLAPHHQCSQLFLYDWYTKVTSLVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSVQRSRQDLFAVDTQTGSVTSLTAGGSAGSWKLLTIDRDLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEPIPDIHWGIRVLHPPPDQENVQYADLDFEAILLQPSNSPDKSQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVQQVLQE--EHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDIPDWCMVETGFPYSNDYLPDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQGLEYYHALKARNVPVRLLLYPKSTHALSEVEVESDSFMNTVLWL 726
BLAST of SMED30008513 vs. UniProt/SwissProt
Match: sp|P19205|ACPH_PIG (Acylamino-acid-releasing enzyme OS=Sus scrofa OX=9823 GN=APEH PE=1 SV=2) HSP 1 Score: 402.134 bits (1032), Expect = 1.854e-124 Identity = 232/730 (31.78%), Postives = 379/730 (51.92%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSPQ--VLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+A+ K+PK SFFQ + D + E+ G+QF + +D+GE + + PV+C+LD++ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + DE+ + SP+ SPD I++L + PH QC +L Y+ T T VVVD+V + E F+G+Y L CW+ DS ++ S + + + +D + +V L + S LL ++ ++V+ S+PS P L +G L A E + V L F ++ + ++L P+ +H ++ +++I + +S ++ PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A E +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN+ SM +TDIPDW E G + D L + M ++SP+ ++K P+L+M+GQ+D+RVP QG+E Y+ L+AR+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGRYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGTGTAEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVADKKRPKAESFFQTKALDVTGSDDEMARTKKPDQAIKGDQFLFYEDWGENMVSKSTPVLCVLDIESGNISVLEGVP--ESVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLTGGKCELLSDESVAVTSPRLSPDQCRIVYLRFPSLVPHQQCGQLCLYDWYTRVTSVVVDIVPRQLGEDFSGIYCSLLPLGCWSADSQRVVFDSPQRSRQDLFAVDTQMGSVTSLTAGGSGGSWKLLTIDRDLMVVQFSTPSVPPSLKVGFLPPAGKEQAVSWVSLEEAEPFPDISWSIRVLQPPPQQEHVQYAGLDFEAILLQPSNSPEKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQE--EHFDAGRVALMGGSHGGFLSCHLIGQYPETYSACVVRNPVINIASMMGSTDIPDWCMVEAGFSYSSDCLPDLSVWAAMLDKSPIKYAPQVKTPLLLMLGQEDRRVPFKQGMEYYRVLKARNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWL 726
BLAST of SMED30008513 vs. UniProt/SwissProt
Match: sp|P13676|ACPH_RAT (Acylamino-acid-releasing enzyme OS=Rattus norvegicus OX=10116 GN=Apeh PE=1 SV=1) HSP 1 Score: 399.053 bits (1024), Expect = 2.510e-123 Identity = 233/730 (31.92%), Postives = 378/730 (51.78%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P+L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ L + SG+E + + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA E+ G+QF + +D+GE + + PV+C+LD+D I VL+ +S G P G+ F G+ + P+ LG+ YC R L+Y L + + + D + + SP+ SPD I++L+ PH QC +L Y+ T T VVVD+V + E F+G+Y L CW+ DS ++ SA + + + +D +T ++ L + + S LL ++ ++ + S+PS P L +G L E + V L + ++L P+ ++ ++ +++I + + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A E +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN+ SM +TDIPDW E G + L + M ++SP+ + ++K P+L+M+GQ+D+RVP QG+E Y+AL+AR+V L +YPK H L+ VEA SDSF+N + W
Sbjct: 7 LSEPQEAAALYRGLSRQPSLSAACLGPEVTTQYGGLYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAV-LRKAGGTVSGEEKQ--FLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDISASDDEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIDSGNISVLEGVP--ENVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCELLSDGSLAICSPRLSPDQCRIVYLQYPCLAPHHQCSQLCLYDWYTKVTSVVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQTGSITSLTAAGSAGSWKLLTIDKDLMVAQFSTPSLPPSLKVGFLPPPGKEQSVSWVSLEEAEPIPGIHWGVRVLHPPPDQENVQYADLDFEAILLQPSNPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQE--EHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIASMMGSTDIPDWCMVETGFPYSNSCLPDLNVWEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQGMEYYRALKARNVPVRLLLYPKSNHALSEVEAESDSFMNAVLWL 726
BLAST of SMED30008513 vs. UniProt/SwissProt
Match: sp|P80227|ACPH_BOVIN (Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2) HSP 1 Score: 385.956 bits (990), Expect = 1.777e-118 Identity = 231/725 (31.86%), Postives = 375/725 (51.72%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKFLNNADPY------IISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P L++A + + WT DL + + D + ++ + E ++ S + + S+G+E + + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D S E+ G+QF + +D+GE + + G+PV+C+LD++ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + D++ + SP+ SPD I++L+ + PH QC +L Y+ T T VVVDVV + E F+G+Y L CW+ DS ++ +A + + + +D + V L + S LL ++ ++ + S+P+ P L +G L A +E E+ V L ++ + ++L PE +H ++ +++I I + PM++M HGGPHS+F T + + A + ++GF AL+VNYRGS G+GQDSI SL G +G DVKD A E +++E D ++ L GGSHGGFL+ HL Y + + RNPVIN+ SM +TDIPDW E G + D L + + M +SP+ ++K P+L+M+GQ+D+RVP QG+E Y+AL+AR+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGRYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGSTGEEKQ--FLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDISGSDDEMARPKKPDQAIKGDQFLFYEDWGENMVSKGSPVLCVLDIESGNISVLEGVP--ESVSPGQAFWAPGDTGVVFAGWWHEPFRLGIRFCTNRRSALYYVDLTGGNCELLSDDSLAVTSPRLSPDQCRIVYLQFPSLVPHQQCGQLCLYDWYTRVTVVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDTAQRSRQDLFAVDTQMGTVTPLTAGGSGGSWKLLTIDRDLMVAQFSTPNLPPCLKVGFLPPAGMEQEVVWVSLEEAEPIPDISWSIRVLQPPPEQEHAQYVGLDFEAILIQPSNPPDKTQVPMVVMPHGGPHSSFVTSWMLLPAMLCKMGFAALLVNYRGSTGFGQDSILSLPGNVGSQDVKDVQFAVEQVLQE--EHFDAGRVALLGGSHGGFLSCHLIGQYPETYGACVVRNPVINIASMMGSTDIPDWCVVEAGYLYSSDCLPDPNVWSEMLNKSPIKYTPQVKTPVLLMLGQEDRRVPFKQGMEYYRALKARNVPVRLLLYPKSTHSLSEVEVESDSFMNAVIWM 724
BLAST of SMED30008513 vs. UniProt/SwissProt
Match: sp|P13798|ACPH_HUMAN (Acylamino-acid-releasing enzyme OS=Homo sapiens OX=9606 GN=APEH PE=1 SV=4) HSP 1 Score: 383.645 bits (984), Expect = 1.265e-117 Identity = 236/730 (32.33%), Postives = 379/730 (51.92%), Query Frame = -3
Query: 1123 MSVLKNITSVYKSYARYPNLNSAKF------LNNADPYIISSTWTVNDLVTSKTKNLNLNFYY---EKDKNLILKSP--QVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEI-----------GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWF 3231
+S + ++Y+ +R P L++A + + WT DL + +N+ Y +++ P + ++ S S +A+ RK+ + E K + +W +KS NL+ ++ HG V++ FG W L+Y+AE K+PK SFFQ + D SA EI G+QF + +D+GE + + PV+C+LDV+ I VL+ +S G P G+ F G+ + P+ LG+ +C R L+Y L + + + D++ + SP+ SPD I++L+ + PH QC +L Y+ T T VVVDVV + E F+G+Y L CW+ DS ++ SA + + + +D + V L + S LL ++ ++ + S+PS P L +G L SA E + V L ++ ++L PE ++ ++ +++I + S + PM++M HGGPHS+F T + +F A + ++GF L+VNYRGS G+GQDSI SL G +G DVKD A E +++E D + + L GGSHGGF++ HL Y + ++ RNPVIN+ SM +TDIPDW E G + D L + M ++SP+ + ++K P+L+M+GQ+D+RVP QG+E Y+AL+ R+V L +YPK H L+ VE SDSF+N + W
Sbjct: 7 LSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDL--ERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVL--RKAGGTGPGEEK-QFLEVWEKNRKLKSFNLSALEKHGPVYEDDC-FGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVP--ENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQE--EHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWL 726
BLAST of SMED30008513 vs. TrEMBL
Match: V4A709 (Peptidase_S9 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_201138 PE=4 SV=1) HSP 1 Score: 468.389 bits (1204), Expect = 2.299e-147 Identity = 259/712 (36.38%), Postives = 400/712 (56.18%), Query Frame = -3
Query: 1117 LKNITSVYKSYARYPNLNSAKFLNNADPYI-ISSTWTVNDLVTSKTKNLNLNFYYE-KDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHD---QSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITEF-TGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFD--HPKFGKKKYQSIFISSKK--EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3222
++ I VY+ A SA+ + D ++ +++ W+ DL S+ N +N+ +D ++++K+P + EI S S K+ A+ K+ K+ I +W + IK+ ++ + HG V+ K FG QW + KL+Y +E K PK SFF + + + K I +G + +D+GEQL VICILD+ IEVLD+ LS G C P + FTG+ N P+ LGL YC QR ++ Y L +++I I D+ +S SP SPD +++ + G H QC RL Y+ T V++D+V P E G+Y L CW++DS I+L+++ + + + I+++TK V LN S +++D+ +++ +SP+T +L +G+L S E E++ L+ DN ++ + H P D H K+G Y++I + K + P+IL HGGPH+ F T + ++ A + GF LMVNYRGS G+GQDS++S+LG IG DVKDC A++++I + VDG K+F++GGSHGGFL +HL Y D ++ CRNPV NL MT TDIPDW + E G + LLN MW+RSP+ VD++K P+L+MIG+DD RVPPSQ E Y+AL+ R++KT L Y +CHP+ V++ +D+FVN++ W+ E
Sbjct: 13 VEEIVGVYRDLASISLPTSAQVRSVDDQFLAVTTQWSQRDLEKSEKTNFTVNYILNAQDYSVLIKTPSQEVKNEI-WKKESPSGKYHAVVTSWKNKKGEEKQ----FIEIWNSQGKIKNYDVCSQEKHGKVNNKDDTFGCLQWSNNEEKLLYTSERKLPKTKSFFGTTNGNDDKKEEKDIVLGKEHYTVEDWGEQLVKKSCSVICILDIKSGTIEVLDTPA---DLSCGQACWGPEDNTVVFTGWNNDPFRLGLKYCTQRQSAVYTYDLKTQKITSISDKTQSSRSPNISPDQSKLVYFDQPIGGAHKQCSRLKMYDFKTQTNQVILDIVHRPKDEVCNGIYEDVLMSRCWSHDSKQIILSTSYRSRTVLYCINVETKTVRQLNGGIKDGSSSIVDIYKDIIIISCTSPNTPQHLMVGKLLSNPGE-EIKWHYLNQP-EIDNNISFHLSSHQPTTDRKHSKYGYLDYETILVLPKTTDDKKPPLILFPHGGPHTTFDTLYMMYVAGFCKCGFAVLMVNYRGSLGFGQDSVESILGNIGDQDVKDCKAAADEIISK--GLVDGQKVFMFGGSHGGFLTTHLIGQYPDFFQAGCCRNPVTNLTVMTGVTDIPDWVYVESGFDYNHSCLLNPSYSEEMWKRSPIRYVDQVKAPLLLMIGKDDLRVPPSQAKEYYRALKTRNIKTRLIEYEDNCHPIVKVDSEADAFVNIMLWYKE 712
BLAST of SMED30008513 vs. TrEMBL
Match: A0A1I8GHA1 (Peptidase_S9 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 446.432 bits (1147), Expect = 1.423e-138 Identity = 252/741 (34.01%), Postives = 401/741 (54.12%), Query Frame = -3
Query: 1108 KNITSVYKSYARYPNLNSAKFLNN---ADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGI-----EIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGEL-KSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFI-------------------SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPS--------KVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYL 3219
K + VY A YP SA+ L +D + V DL + K + L +L+ P VL D + DVY S HRA+ L+ K+ S + ++H++ + FIKSI+L++ HG V+ G QW + L+Y+AE K+PK S+F + + + G +G Q+ + FGEQL+ PV+C LDVD E + VLD + G+ C P+ G+ F GY+ +PY LG+IYC QR ++Y S E IG + ++ SP+FSP ++WL+ + SGPH QC+RL+ + AT ++D V + + F G+Y L CW+ + ++L+S + ++ ++ ID+KTK +L++ +S +LD + +L+V+ S+P+ P++A G + S L + ++ L+ ++ +++ ++ H + HP+FG + ++SI + ++ E P+I HGGP+++F + + +A G+ LM+NYRGS G+G+ ++DSLLG IG+ DV DCI+A E+ + E S ++DG K+ +GGSHGGFL HL K+A RNPVINL +M +DIPDW + + G+ + D LL+ E + +W +SPLS K+ P L+ +G D RVPP QG ELY+ALRAR+V T L + P DCHP++SV S FV+++ WF +
Sbjct: 11 KEVAQVYSDLAAYPVPQSAQLLPQYPFSDAIRTRCAYQVQDLASQKNRTLVKTAVVLPTGDLVDSDP-VLRDPTTLWDVYCSVGDHRAVLLKLKAEGSGSDNADKFIVHVYNESRFIKSIDLSEKAKHGAVYGP--ELGACQWSYNEGHLLYVAEKKQPKNASYFAADTFSEESAGKPGEAKSVGKQYDSVEHFGEQLSEYKRPVLCTLDVDSEVVTVLDDGGILDGVLPTEPCWAPDDAGVVFVGYKELPYQLGVIYCNQRPSAIYYLDFRSGECAVIGHDTRAVSSPRFSPCQTQLVWLQNEPSGPHKQCRRLIWCDWATKDIRFIIDFVNQAGSAGFPGIYADSLPSRCWSSNCRTVVLSSINRSKDCLLAIDVKTKIGELLSLPPDASSAKVLDFHRDWLVVEASAPNVPPFIAAGFIDASKPLTSNIKWTCLTDCFKLNDI-AWRVKQHTAKLAHPEFGNQLFESILVWPVDSSKKKDSKKGDEEKPAAITELPLPLIAFPHGGPNTSFTATYMRYVCGLARCGYAVLMINYRGSLGFGKSTVDSLLGGIGERDVSDCIQAVEETLAESASSADNSALPRLDGEKLAAFGGSHGGFLGGHLIGQRPGMFKAAVLRNPVINLATMVGGSDIPDWVYEQSGLPFSHDRLLDAETLALLWSKSPLSHASKVNTPTLVAVGLKDLRVPPQQGRELYRALRARNVPTRLLVCPDDCHPMSSVPVQSSVFVDIVLWFNRHVM 747
BLAST of SMED30008513 vs. TrEMBL
Match: A0A267GS90 (Peptidase_S9 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010596g3 PE=4 SV=1) HSP 1 Score: 446.817 bits (1148), Expect = 1.799e-138 Identity = 252/741 (34.01%), Postives = 401/741 (54.12%), Query Frame = -3
Query: 1108 KNITSVYKSYARYPNLNSAKFLNN---ADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGI-----EIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGEL-KSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFI-------------------SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPS--------KVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYL 3219
K + VY A YP SA+ L +D + V DL + K + L +L+ P VL D + DVY S HRA+ L+ K+ S + ++H++ + FIKSI+L++ HG V+ G QW + L+Y+AE K+PK S+F + + + G +G Q+ + FGEQL+ PV+C LDVD E + VLD + G+ C P+ G+ F GY+ +PY LG+IYC QR ++Y S E IG + ++ SP+FSP ++WL+ + SGPH QC+RL+ + AT ++D V + + F G+Y L CW+ + ++L+S + ++ ++ ID+KTK +L++ +S +LD + +L+V+ S+P+ P++A G + S L + ++ L+ ++ +++ ++ H + HP+FG + ++SI + ++ E P+I HGGP+++F + + +A G+ LM+NYRGS G+G+ ++DSLLG IG+ DV DCI+A E+ + E S ++DG K+ +GGSHGGFL HL K+A RNPVINL +M +DIPDW + + G+ + D LL+ E + +W +SPLS K+ P L+ +G D RVPP QG ELY+ALRAR+V T L + P DCHP++SV S FV+++ WF +
Sbjct: 33 KEVAQVYSDLAAYPVPQSAQLLPQYPFSDAIRTRCAYQVQDLASQKNRTLVKTAVVLPTGDLVDSDP-VLRDPTTLWDVYCSVGDHRAVLLKLKAEGSGSDNADKFIVHVYNESRFIKSIDLSEKAKHGAVYGP--ELGACQWSYNEGHLLYVAEKKQPKNASYFAADTFSEESAGKPGEAKSVGKQYDSVEHFGEQLSEYKRPVLCTLDVDSEVVTVLDDGGILDGVLPTEPCWAPDDAGVVFVGYKELPYQLGVIYCNQRPSAIYYLDFRSGECAVIGHDTRAVSSPRFSPCQTQLVWLQNEPSGPHKQCRRLIWCDWATKDIRFIIDFVNQAGSAGFPGIYADSLPSRCWSSNCRTVVLSSINRSKDCLLAIDVKTKIGELLSLPPDASSAKVLDFHRDWLVVEASAPNVPPFIAAGFIDASKPLTSNIKWTCLTDCFKLNDI-AWRVKQHTAKLAHPEFGNQLFESILVWPVDSSKKKDSKKGDEEKPAAITELPLPLIAFPHGGPNTSFTATYMRYVCGLARCGYAVLMINYRGSLGFGKSTVDSLLGGIGERDVSDCIQAVEETLAESASSADNSALPRLDGEKLAAFGGSHGGFLGGHLIGQRPGMFKAAVLRNPVINLATMVGGSDIPDWVYEQSGLPFSHDRLLDAETLALLWSKSPLSHASKVNTPTLVAVGLKDLRVPPQQGRELYRALRARNVPTRLLVCPDDCHPMSSVPVQSSVFVDIVLWFNRHVM 769
BLAST of SMED30008513 vs. TrEMBL
Match: A0A098M0X3 (Acylamino-acid-releasing enzyme OS=Hypsiglena sp. JMG-2014 OX=1550645 PE=4 SV=1) HSP 1 Score: 440.654 bits (1132), Expect = 1.159e-136 Identity = 246/727 (33.84%), Postives = 392/727 (53.92%), Query Frame = -3
Query: 1117 KNITSVYKSYARYPNLNSAKFLNNADPYI------ISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEI---IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFF----------QNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTT--LCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNV-LNCQILDHIPEFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3219
+ I ++Y+ ++YP+L+ A I + W+ DL ++ + D I+ S E+ ++ S S +A+ RK +S G+E + + +W +KSI L ++ HG V+ FG W L+Y+AE K+PK SFF + ++ + K ++ G QF + +D+GE L PV+CILD++ + VL+ + PG + T PN G+ FTG+ + P+ LGL YCI R LF+ L + + + ++NK+ SP+ SPD I++LE ASGPH QC R+ Y+ T T V+VD+V I F G+Y+ L E+CW DS ++L + + + ++I++D+ TK+V L S + S LL ++ ++ K S+P++ P L + L A E ++ V L + + +L P+ D+P++ +++I + + + P+++ HGGPHS F T + ++ A + +GF L+VNYRGS G+GQDS+DSL G +G DVKD E M+++ P +D ++ L GGSHGGFL+ HL Y K+ RNPV+N+ SM +TDIPDW TE G+ + L E M SP+ VDK++ P+L++IG++D+RVPP QGLE Y+ALR+R + T + YP D H L+SVEA +D F+N+ W +
Sbjct: 10 EEIAALYRDLSQYPSLSHACIGPEVTTQYGGRHCNIYTEWSQRDLERAENVKFCRQYLVFHDNQSIIYSGPSGNCTEVKGELLSRDSPSGTQKAVV--RKMKNSKGEEK--HFLEVWEKNRKVKSIELTALEKHGQVYDDD-QFGCLAWSHSETHLLYVAEKKRPKAESFFKPRASELNTEEELAKTERDKAVK-GEQFVFYEDWGETLVAKSIPVLCILDIESSNVSVLEGVPEYISPGQAFWT----PNDTGVVFTGWYHEPFRLGLKYCINRRSSLFHVDLTGGQCELLSEDNKAIWSPRLSPDQCRIVYLENQASGPHQQCSRICMYDWYTKLTSVIVDIVPRQIQGGFAGIYSTALPEHCWAADSQRVVLDTTQRSRQELIVVDMATKSVCSLTKSSFLGSWVLLTIDRDLMVAKFSTPNSPPMLMVAFLPPAGKEADVNWVCLEETSPIQGISWDVHVLQPSPDQDNPQYRGLDFEAILLKPTQVSEQAKLPLVVSPHGGPHSVFTTAWMLYPAVLCRMGFAVLLVNYRGSLGFGQDSVDSLPGNVGCQDVKDVQFCVEQMLQKEP--LDSKRVALLGGSHGGFLSCHLIGQYPSTYKACVTRNPVVNMASMIGSTDIPDWCITEVGLSYDQAALPVPEQWEKMLLHSPMQYVDKVQAPVLLLIGEEDRRVPPKQGLEYYRALRSRGIPTRMLQYPSDNHALSSVEAEADGFMNIALWLLQ 724
BLAST of SMED30008513 vs. TrEMBL
Match: A0A267FM49 (Peptidase_S9 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009883g1 PE=4 SV=1) HSP 1 Score: 439.884 bits (1130), Expect = 1.031e-135 Identity = 251/741 (33.87%), Postives = 401/741 (54.12%), Query Frame = -3
Query: 1108 KNITSVYKSYARYPNLNSAKFLNN---ADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGI-----EIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGEL-KSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFI-------------------SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPS--------KVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYL 3219
K + VY + A YP SA+ L +D + V DL + K + L +L+ P VL D + DVY S HRA+ L+ K+ S + ++H++ + FIKSI+L++ HG V+ G QW + L+Y+AE K+PK S+F + + + G +G Q+ + FGEQL+ PV+C LDVD E + VLD + G+ C P+ G+ F GY+ +PY LG+IYC QR ++Y S E IG + ++ SP+FSP ++WL+ + SGPH QC+RL+ + AT ++D V + + F G+Y L CW+ + ++L+S + ++ ++ ID+KTK +L + +S +LD + +L+V+ S+P+ P++A G + S L + ++ L+ ++ +++ ++ H + HP+FG + ++SI + ++ E P+I HGGP+++F + + +A G+ L++NYRGS G+G+ ++DSLLG IG+ DV DCI+A E+ + E S ++DG K+ +GGSHGGFL HL K+A RNPVINL +M +DIPDW + + G+ + D LL+ E + +W +SPLS K+ P L+ +G D RVPP QG ELY+ALRAR+V T L + P DCHP++SV S FV+++ WF +
Sbjct: 33 KEVAQVYSNLAAYPVPQSAQLLPQYPFSDAIRTRCVYQVQDLASQKNRTLVKTAVVLPTGDLVDSDP-VLRDPTTLWDVYCSGGDHRAVLLKLKAEGSGSDNADKFVVHVYNESRFIKSIDLSEKAKHGAVYGP--ELGACQWSCNEGHLLYVAEKKQPKNASYFAADAFSEESAGKPGEAKSVGKQYDSVEHFGEQLSEYKRPVLCTLDVDSEVVTVLDDG-IPDGVLPTEPCWAPDDAGVVFVGYKELPYQLGVIYCNQRPSAIYYLDFRSGECAVIGHDTRAVSSPRFSPCQTQLVWLQNEPSGPHKQCRRLIWCDWATKDIRFIIDFVNQAGSAGFPGIYADSLPSRCWSSNCRTVVLSSINRSKDCLLAIDVKTKIGELLPLPPDASSAKVLDFHRDWLVVEASAPNVPPFIAAGFIDASKPLTSNIKWTCLTDCFKLNDI-AWRVKQHTAKLAHPEFGNQLFESILVWPVGSSKKKDSKKGDEEKPAAITELPLPLIAFPHGGPNTSFTATYMRYVCGLARCGYAVLVINYRGSLGFGKSTVDSLLGGIGERDVSDCIQAVEETLAESASSADNSALPRLDGEKLAAFGGSHGGFLGGHLIGQRPGMFKAAVLRNPVINLATMVGGSDIPDWVYEQSGLPFSHDRLLDAETLALLWSKSPLSHASKVNTPTLVAVGLKDLRVPPQQGRELYRALRARNVPTRLLVCPDDCHPMSSVPVQSSVFVDIVLWFNRHVM 768
BLAST of SMED30008513 vs. Ensembl Cavefish
Match: apeh (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:103034957]) HSP 1 Score: 397.897 bits (1021), Expect = 1.384e-123 Identity = 252/768 (32.81%), Postives = 401/768 (52.21%), Query Frame = -3
Query: 1117 IFAFKSGLFIYLIYSIFKFFD*NMSVLKNITSVYKSYARYPNLNSAKFLNNADPYI----------ISSTWTVNDLVTSKTKNLNLNF-----YYEKDKNLILKSPQVLMDE--EIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHD----------QSAKGIEI--GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP--EFDHPKFGKKKYQSIFISSK--KEGS-CPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDY-----LLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3300
IF+ + + + + D ++ ++I +Y+ +++P+L+ A + P I IS+ WT DL +N N+ F + DK+++ P E ++ S S +A+ ++ G+E + + +W IKS+NL + HG V++ FG W L+Y+AE K+PK S+F++ S ++ K E+ G+QF + +D+GE L + PV+C+LD++ + VL+ L +S G PN G+ F G+ + P+ LGL YC R LFY L + +++ ++ + SP+ SPD I++LE GPH QC +L Y+ T T VVVDVVK + FTG+Y+ L CW+ DS +L++ A + + ++++D + V + + S LL ++ ++V SSP+ P L +G L ++ E+E+ V L + + QIL P E D+ ++ + +I + K KEG+ P+I+ HGGPHS F ++ + A + +GF L VNYRGS GYGQDSI SL G +G DVKD A E ++++ + K+ + GGSHGGFLA HL Y D K +NPVINL SM +TDIPDW E G FDY L++ + M +SP+ V ++K P+L+ +G+DDKRVP QG+E Y++L+A V L YP++ H L+ V+A SD F+N+ W +
Sbjct: 11 IFSARRIVLLNWCVRFSRVCDKVLTTPEDIAKLYREQSQFPSLSRA----DVGPVITSQYGGKYSNISTEWTQRDL----ERNENVKFCRQYILFHDDKSIVYSGPSGNCTEIKGELLSQDSPSGDMKAVV---RACTIKGEEKQ--FLEIWHKNSKIKSLNLTALKKHGKVYEDD-QFGCLVWSHSETHLLYVAEKKRPKAESYFESGSEPSLLGDEEEPIKTDKKEEVVKGDQFVFYEDWGEALVSKSTPVLCVLDIEGNNVSVLEG--LPDNISPGQAFWAPNDTGVVFVGWWHEPFRLGLKYCPNRRSSLFYVDLTGGKCEQLSSDSAAVCSPRLSPDLCRIVYLECGVYGPHQQCSKLCMYDWYTKKTSVVVDVVKRAGEDGFTGIYSSMLPHQCWSADSQRVLVSCAQRSRKDLLVVDTSSGQVTSITSKSEMGSWCLLTIHSDLMVVGCSSPNCPPSLRVGFLPASGSESEVAWVTLEEAQK-QAEFDWQILTFTPPSEEDNSQYPGLDFDAILLKPKGVKEGTKLPLIVTPHGGPHSVFVAEWFLSEAVLCRMGFGVLQVNYRGSIGYGQDSILSLPGNVGTQDVKDVQFAVESVLKQ--GDFNREKVAISGGSHGGFLACHLIGQYPDFYKVCIAQNPVINLASMVGSTDIPDWCMVEAG----FDYNTNNALMSPAILEQMLNKSPIKFVTQVKTPVLLTLGEDDKRVPNKQGIEYYRSLKALQVPVRLLWYPENNHSLSKVDAESDGFMNIALWIIQ 755
BLAST of SMED30008513 vs. Ensembl Cavefish
Match: apeh (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:103034957]) HSP 1 Score: 397.127 bits (1019), Expect = 1.656e-123 Identity = 249/745 (33.42%), Postives = 393/745 (52.75%), Query Frame = -3
Query: 1117 MSVLKNITSVYKSYARYPNLNSAKFLNNADPYI----------ISSTWTVNDLVTSKTKNLNLNF-----YYEKDKNLILKSPQVLMDE--EIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHD----------QSAKGIEI--GNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP--EFDHPKFGKKKYQSIFISSK--KEGS-CPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDY-----LLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3231
++ ++I +Y+ +++P+L+ A + P I IS+ WT DL +N N+ F + DK+++ P E ++ S S +A+ ++ G+E + + +W IKS+NL + HG V++ FG W L+Y+AE K+PK S+F++ S ++ K E+ G+QF + +D+GE L + PV+C+LD++ + VL+ L +S G PN G+ F G+ + P+ LGL YC R LFY L + +++ ++ + SP+ SPD I++LE GPH QC +L Y+ T T VVVDVVK + FTG+Y+ L CW+ DS +L++ A + + ++++D + V + + S LL ++ ++V SSP+ P L +G L ++ E+E+ V L + + QIL P E D+ ++ + +I + K KEG+ P+I+ HGGPHS F ++ + A + +GF L VNYRGS GYGQDSI SL G +G DVKD A E ++++ + K+ + GGSHGGFLA HL Y D K +NPVINL SM +TDIPDW E G FDY L++ + M +SP+ V ++K P+L+ +G+DDKRVP QG+E Y++L+A V L YP++ H L+ V+A SD F+N+ W +
Sbjct: 12 LTTPEDIAKLYREQSQFPSLSRA----DVGPVITSQYGGKYSNISTEWTQRDL----ERNENVKFCRQYILFHDDKSIVYSGPSGNCTEIKGELLSQDSPSGDMKAVV---RACTIKGEEKQ--FLEIWHKNSKIKSLNLTALKKHGKVYEDD-QFGCLVWSHSETHLLYVAEKKRPKAESYFESGSEPSLLGDEEEPIKTDKKEEVVKGDQFVFYEDWGEALVSKSTPVLCVLDIEGNNVSVLEG--LPDNISPGQAFWAPNDTGVVFVGWWHEPFRLGLKYCPNRRSSLFYVDLTGGKCEQLSSDSAAVCSPRLSPDLCRIVYLECGVYGPHQQCSKLCMYDWYTKKTSVVVDVVKRAGEDGFTGIYSSMLPHQCWSADSQRVLVSCAQRSRKDLLVVDTSSGQVTSITSKSEMGSWCLLTIHSDLMVVGCSSPNCPPSLRVGFLPASGSESEVAWVTLEEAQK-QAEFDWQILTFTPPSEEDNSQYPGLDFDAILLKPKGVKEGTKLPLIVTPHGGPHSVFVAEWFLSEAVLCRMGFGVLQVNYRGSIGYGQDSILSLPGNVGTQDVKDVQFAVESVLKQ--GDFNREKVAISGGSHGGFLACHLIGQYPDFYKVCIAQNPVINLASMVGSTDIPDWCMVEAG----FDYNTNNALMSPAILEQMLNKSPIKFVTQVKTPVLLTLGEDDKRVPNKQGIEYYRSLKALQVPVRLLWYPENNHSLSKVDAESDGFMNIALWIIQ 733
BLAST of SMED30008513 vs. Ensembl Cavefish
Match: apeh (acylaminoacyl-peptide hydrolase [Source:NCBI gene;Acc:103034957]) HSP 1 Score: 386.341 bits (991), Expect = 8.593e-120 Identity = 249/737 (33.79%), Postives = 386/737 (52.37%), Query Frame = -3
Query: 1117 MSVLKNITSVYKSYARYPNLNSAKFLNNADPYI----------ISSTWTVNDLVTSKTKNLNLNF-----YYEKDKNLILKSPQVLMDE--EIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQ----NKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP--EFDHPKFGKKKYQSIFISSK--KEGS-CPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDY-----LLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3231
++ ++I +Y+ +++P+L+ A + P I IS+ WT DL +N N+ F + DK+++ P E ++ S S +A+ ++ G+E + + +W IKS+NL + HG V++ FG W L+Y+AE K+PK S+F+ H +G+QF + +D+GE L + PV+C+LD++ + VL+ L +S G PN G+ F G+ + P+ LGL YC R LFY L + +++ ++ + SP+ SPD I++LE GPH QC +L Y+ T T VVVDVVK + FTG+Y+ L CW+ DS +L++ A + + + L D N L + + S LL ++ ++V SSP+ P L +G L ++ E+E+ V L + + QIL P E D+ ++ + +I + K KEG+ P+I+ HGGPHS F ++ + A + +GF L VNYRGS GYGQDSI SL G +G DVKD A E ++++ + K+ + GGSHGGFLA HL Y D K +NPVINL SM +TDIPDW E G FDY L++ + M +SP+ V ++K P+L+ +G+DDKRVP QG+E Y++L+A V L YP++ H L+ V+A SD F+N+ W +
Sbjct: 7 LTTPEDIAKLYREQSQFPSLSRA----DVGPVITSQYGGKYSNISTEWTQRDL----ERNENVKFCRQYILFHDDKSIVYSGPSGNCTEIKGELLSQDSPSGDMKAVV---RACTIKGEEKQ--FLEIWHKNSKIKSLNLTALKKHGKVYEDD-QFGCLVWSHSETHLLYVAEKKRPKAESYFEVRDH--------LGDQFVFYEDWGEALVSKSTPVLCVLDIEGNNVSVLEG--LPDNISPGQAFWAPNDTGVVFVGWWHEPFRLGLKYCPNRRSSLFYVDLTGGKCEQLSSDSAAVCSPRLSPDLCRIVYLECGVYGPHQQCSKLCMYDWYTKKTSVVVDVVKRAGEDGFTGIYSSMLPHQCWSADSQRVLVSCAQRSRKVFNSSRCLGDSHHSN---LCLGSEMGSWCLLTIHSDLMVVGCSSPNCPPSLRVGFLPASGSESEVAWVTLEEAQK-QAEFDWQILTFTPPSEEDNSQYPGLDFDAILLKPKGVKEGTKLPLIVTPHGGPHSVFVAEWFLSEAVLCRMGFGVLQVNYRGSIGYGQDSILSLPGNVGTQDVKDVQFAVESVLKQ--GDFNREKVAISGGSHGGFLACHLIGQYPDFYKVCIAQNPVINLASMVGSTDIPDWCMVEAG----FDYNTNNALMSPAILEQMLNKSPIKFVTQVKTPVLLTLGEDDKRVPNKQGIEYYRSLKALQVPVRLLWYPENNHSLSKVDAESDGFMNIALWIIQ 709
BLAST of SMED30008513 vs. Ensembl Cavefish
Match: zgc:136971 (zgc:136971 [Source:ZFIN;Acc:ZDB-GENE-060421-3674]) HSP 1 Score: 313.538 bits (802), Expect = 4.496e-93 Identity = 197/639 (30.83%), Postives = 318/639 (49.77%), Query Frame = -3
Query: 1117 IMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFK--YKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVN-ILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSS---IYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFI---SSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3003
++ +S S RA+ E +S + +W KS +L + HG V++ + F W KL+Y+AE K+ S +QS + I + + K Y +D+GE L PV+C+ D+ + ++ V T + P +S G G G+ F G+ N P+ LGL +C R F+ L + + ++ S SP+ SPD R++++L+ GPH QC +M Y+I + V++DV++ +F G+Y+ L CW +S I +S + +D +T + + + S LL + + L++ SSP+ P L +G + S + E++ L +FD I+ PE ++ +F + ++ + SS K G P+++ +H VNYRGS G+GQDSIDSLLG +G DVKD +A +++ +D +++ + GGSHGGFLA HL Y + ++ A RNPVIN ++ +DI DW ++ G+ + FD L E + TM E+SP++ +I P+L+M+G DKRV P QGLELYKAL++R L Y D H L+ +E SD F+N+ WF +
Sbjct: 82 LLSSHSPSGTRRAVIRE-----ASAHGVGHQYLEVWGCRGLEKSFDLTALSKHGKVYEDA-QFACLAWSPCEEKLLYVAEKKRVGSSEKASPSSANQSGELIVLEEEDKNVYMEDWGEGLVGKSVPVLCVADLSKREVNVC--TGVPPHVSPGQALWAEGGRGVVFVGWWNEPFRLGLKFCSNRRSARFHIDLKGN-CELLSADSGSVSSPRLSPDHRWLVYLQGRVFGPHHQCLLMMLYDIEKRSSSVLIDVIRRAGKGQFAGVYD-PLPPQCWASNSEEIFFSSPCGNSKVVFSVDRQTGRIRPVYDTKREFGSARLLTVINDLLVISCSSPNHPPSLVVGFVSSRENKQEVQWTHLGGADVCEKFD--WQDIIITPPPEEENHQFSGLTFGAVLLKPDSSSKSGKIPLVVFIH---------------------------VNYRGSTGFGQDSIDSLLGNVGSQDVKDVQRAVLHILQS-DQALDADRVAVMGGSHGGFLACHLIGQYPEFYRACAARNPVINAATLVGTSDIVDWRYSSVGLHYSFDQLPTPEALITMLEKSPIAHAAQICAPVLLMLGGKDKRVSPYQGLELYKALKSRKSPVRLLWYDGDGHSLSRLETQSDCFLNIALWFQQ 680
BLAST of SMED30008513 vs. Ensembl Cavefish
Match: ENSAMXT00000041345.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:32334536:32339002:1 gene:ENSAMXG00000029230.1 transcript:ENSAMXT00000041345.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 42.3578 bits (98), Expect = 3.116e-12 Identity = 26/76 (34.21%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 524 VKNAS*KFQRCMDKIISPLREFSG-TYIGDIIVLSTSKKEHEKNLDLIREALKQENLSIKEEKSNIESISRQLDYL 748
+KNA+ FQR MD ++ L+ S YI DI+V S++ ++H +L+ + L Q L++ K N+ + R L +L
Sbjct: 754 IKNAAATFQRLMDSVLVNLKGKSCFVYINDIVVYSSTIEQHLGHLEEVFRCLHQAGLTLNLRKCNL--LQRSLIFL 827 HSP 2 Score: 36.5798 bits (83), Expect = 3.116e-12 Identity = 17/36 (47.22%), Postives = 22/36 (61.11%), Query Frame = 3
Query: 423 KKAYHQIPIALDYIPKTAVITHYGLYEYVKMTFALK 530
K Y Q+ + D IPKTA IT GLYE+ + F +K
Sbjct: 720 KSGYWQVGLEPDSIPKTAFITCQGLYEFTVLPFGIK 755 HSP 3 Score: 32.7278 bits (73), Expect = 3.116e-12 Identity = 14/33 (42.42%), Postives = 22/33 (66.67%), Query Frame = 1
Query: 328 VQTCGDYRGLNA-LRVDRYPLPRITGILDQLRG 423
V+ C DYR +N+ +D YP+P++ IL+ L G
Sbjct: 678 VRFCVDYRRMNSKTHLDAYPMPQVQEILESLHG 710
BLAST of SMED30008513 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001392.1 (pep scaffold:Pmarinus_7.0:GL482689:14672:17070:1 gene:ENSPMAG00000001250.1 transcript:ENSPMAT00000001392.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 75.0998 bits (183), Expect = 4.402e-15 Identity = 62/252 (24.60%), Postives = 113/252 (44.84%), Query Frame = -3
Query: 2551 NMSVLKNITSVYKSYARYPNLNSAKF-----LNNADPY-IISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSS-SYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQN-----------------VSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIG 3234
N+ +K + + ++ A+ P A+ + Y ++ + W+ D+ +++ +L++ +D I+ + E+ ++ S S + RK G + K + +W L K+I+L ++ HG + FG W L+Y+AE K+PK +FFQ+ + Q AKG ++F +++D+GE L N +PV+CILDV + VLD L P +S G
Sbjct: 6 NLKHVKVVMAAFRDVAKQPVALRARIGPQMRSRHGGAYSLVHTEWSYRDVERAESVVCSLSYSVFRDGATIVDTSPAGGPTEMHAELKRSVSPSGTLTAILRKIGGKGGADEK-QFLEIWNGNLKEKNIDLEALEQHGKFYDDG-QFGVLAWSNSESHLLYVAEKKRPKGTTFFQSRPPSANTAQTDAPTDPGTTDRQKAKG----DKFLFREDWGEALVNKSDPVLCILDVVSGTVSVLDG--LPPHISPG 249
BLAST of SMED30008513 vs. Ensembl Nematostella
Match: EDO40564 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6Q1]) HSP 1 Score: 377.481 bits (968), Expect = 9.051e-117 Identity = 224/730 (30.68%), Postives = 378/730 (51.78%), Query Frame = -3
Query: 1117 VLKNITSVYKSYARYPNLNSAKFLNNAD-----PYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLIL---KSPQVLMD--EEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQ------SAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPG----LSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNL-GLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENE-MRLVPLSSIYRFDNVLNCQI---LDHIPEFDHPKFGKKKYQSIFISS-KKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3225
VL+ V++ A P L A L+N +I++S WT DL + N N +++ + L K P +L + V S S K++ I R ++S E K + +W I+SI++ + D HG+++ S F F W + ++Y+AE KKPK+LS+F+ + G+ + Y++D+GEQL PV+ +L V +I T+ PG LS+G C + G+ F+G+ + PY LGL+YC R +F+ +D ++++ ++ P +P + +L D G H C +L++Y T ++D V P + F G Y L+ CW+ D ++L S + I+ +D+ ++ +++ +LD+ LL SSPS + + + + L E + ++S + L I ++ P + + Y+ + + KE P+I++ HGGP S + + + + A+ +LGF L VNY+GS G+G+ S+ S++G +G DV++ + A+E+++ D + +F+ GGSHGGFL++HL Y D+ ++ A RNPVI++ SM T TDIPDW F ECG + ++ + + + MWE+SP++ V K++ P+L+ IG D+RVPPSQG+ ++ LR R V+T+L +YP+D HPL V SD FVN + WF E
Sbjct: 13 VLERAAHVFRELAAIPYLTKANILSNTQNEKPASFIVTSQWTQRDL------DRNENRVFQRSHCVTLSEGKEPSILETGFPAELHKVTSPSRKYQLIL--RGVSESKQYEDK-QFLEIWSQNNIIRSIDVKEADKHGLIYTDSL-FSCFNWSASEKYVLYVAEKKKPKRLSYFEKDKEGNIKIFFNNYSSSSSGDHYLYEEDWGEQLVKKNCPVLAMLAVHSGEI------TIIPGIPDYLSVGQACWTLDDKGVVFSGWWHKPYRLGLVYCSNRRGGVFHVKVDGSGLEQLSSPEETVAYPNINPITGKLYYLARDCIGVHQTCWKLVEYNWKTKANTTIIDYVDVPGDDGFPGFYMFNSLSSRCWSKDGTKLILASIWRSSQHILCVDVN--KCTLVRLTKAPGCWDVLDVYQDLLLASFSSPSQPHQIYLMHIPTELSSMEDLGCTEIASSANGSSDLFNDIEWKIETFPSLETGEGFNADYEGVLVQPIGKETKPPLIVLPHGGPQSVYNSSYIISSVAMCKLGFAILWVNYKGSVGFGRKSLQSIIGKVGTQDVREVMAAAENVLSR--GAHDPHNLFVMGGSHGGFLSAHLIGQYPDKFRACAARNPVIDISSMVTVTDIPDWCFVECGFDFDYNLATDSKTMTDMWEKSPIAHVHKVRTPVLLCIGAVDRRVPPSQGIHFHRVLRERGVETKLLLYPEDAHPLDKVGTESDVFVNTVRWFHE 722
BLAST of SMED30008513 vs. Ensembl Medaka
Match: apeh (acylamino-acid-releasing enzyme [Source:NCBI gene;Acc:101160809]) HSP 1 Score: 402.134 bits (1032), Expect = 1.746e-125 Identity = 243/736 (33.02%), Postives = 377/736 (51.22%), Query Frame = -3
Query: 1117 KNITSVYKSYARYPNLNSAKFLNNADPYIIS----------STWTVNDLVTSKTKNLNLNF-----YYEKDKNLILKSPQVLMDE--EIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSA------------KGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITE-FTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIP--EFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3219
+ I +Y+ + YP+L+ A + P I S + W+ DL +N N+ F + DK+L+ E ++ S S + +A+ E + K + + +W + +KSINL + HG V++ FG W L+Y+AE K+ K SFFQ V + S+ K G QF Y +D+GE L N PV+C+LD++ + + VL+ +S G P G+ F G+ + P+ LGL +C R LFY L + +++ + + SP+ SPD II+LE GPHMQC ++ Y+ T T VVVD VK + F G+Y L+ CW+ DS +L++ + + ++ +DI T +V L + S LL++ ++V SSP+ PYL +G L S + ++ V L ++ QI+ P E D+ ++ ++++ I K+ EG P++++ HGGPHS ++ + + + +GF L+VNYRGS G+GQD+I SLLG +G DVKD A E +++ + DG K+ + GGSHGGFLA HL Y K+ RNPV NL SM +TDIPDW E G + D L M +SP+ V +++ P+L+ +G+DDKRVP QG+E YKAL+AR + L YP + H L+ V+A SD F+N+ W +
Sbjct: 10 EEIAKLYRELSLYPSLSRA----DVGPVITSQYGGKYSNIYTEWSQRDL----ERNENVKFCRQYIVFHDDKSLVYSGASGNCTEIRGEVLSKDSPSGEMKAVVRE-----CTIKGEEKQFLEIWSKNIKMKSINLTALKKHGKVYEDE-QFGCLVWSHSETHLLYVAEKKRLKAESFFQMVPPELSSSIDEEETTRLDKKETVKGEQFAYHEDWGEALVNKSCPVLCVLDIEGDNVSVLEGVP--DDISPGQAFWAPGDTGVVFVGWWHEPFRLGLKFCPNRRSSLFYVDLTGGKCEQLSSGSSAVCSPRLSPDQCRIIYLECSVYGPHMQCSKVCMYDWYTKKTSVVVDEVKRSKEDGFAGIYCSQLSPKCWSADSQRVLISCPQRSRKDLLTVDISTGSVTSLTAESDIGSWCLLNIERDLMVVSCSSPNCPPYLRVGFLPSRDSQEKVVWVTLEGSQMLPDI-GWQIMTFTPPPEQDNSQYPGIDFEALLIKPKEVKAEGKLPLVVIPHGGPHSVIVAEWILSSSVLCSMGFAVLLVNYRGSLGFGQDNILSLLGNVGSQDVKDVQFAVESVLKN--GEFDGQKVAVSGGSHGGFLACHLIGQYPGFYKACVARNPVTNLASMVGSTDIPDWCVVEAGYDYSHDCLPEPSVWEQMLNKSPIKHVTQVQTPVLLTLGEDDKRVPNKQGIEYYKALKARQIPVRLLWYPGNNHSLSKVDAESDGFMNIALWIIQ 726
BLAST of SMED30008513 vs. Ensembl Medaka
Match: zgc:136971 (acylamino-acid-releasing enzyme [Source:NCBI gene;Acc:101170588]) HSP 1 Score: 338.961 bits (868), Expect = 2.367e-102 Identity = 219/724 (30.25%), Postives = 357/724 (49.31%), Query Frame = -3
Query: 1117 NITSVYKSYARYPNLNSAKFLNNADP----YIISSTWTVNDLVTSKTKNLNLNFYYEKDKN-------LILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFK--YKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNV-----NILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGEL--KSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 3216
I VY + +P SA P Y +++ W+ +DLV + + + D N ++ P + E++ +S +AI E SG + L+ +W K +NL ++IHG V+ + FG+ W + KL+Y+AE S + SA G + + Y +D+GE LT+ PV+C++D+ ++VL +S G P+ + F G+ + P+ LGL +C R LF ++ + +++ N S P+ SPDG +I+L+ GPH QC L + ++ + T ++DV+ P EF G+Y L+ CW+ DS ++ +SA + + ++D K+K V N+ S S LL + ++V SSP+T P L +G L KS + + P + FD + PE D+ ++ + S+ + + + P+++ +HGGPHS F ++ A + +LG LMVNYRGS G+GQDSI SL+G IG DVKD + + +D ++ + GGSHGGFL HL Y D K+ A RNPVIN ++ +DI DW +T G ++ +D + E + + ++SP++ +IK +L+M+G D+RV P QGLELYK L+ R+ L +P+D H L+ V+ +D FVN W +
Sbjct: 5 QIAGVYAEVSGFPTPVSAHVKVEELPEYRVYSVTADWSQSDLVRGSRLHYSQQWTLIADTNNHKTVRTVLPPGPFTPVSGELLR-AFSPIRGLKAIVRE------SGNQ---QLLEIWDCHGLRKCLNLTALNIHGRVYDDA-QFGSLSWSECEDKLLYVAEK------------SRNSSAGGESACREDRNVYCEDWGEALTSKSVPVVCVVDLHSGVVDVLQGVP--EDVSPGQALWAPDYQSVFFVGWYHEPFRLGLKFCSNRSSSLFVLDMEGR-CERLSGANLSVSCPRLSPDGSTLIFLQGRVFGPHHQCLSLQQLDLKSRKTSALIDVLNRPQAGEFAGVYET-LSPCCWSADSQRVVFSSACRNWKDLFVVDRKSKKVLSLSDNLSAASRDCGSWKLLTIQRDLMVVCCSSPNTPPTLRVGFLPEKSEAMTWQTLQQPAMT---FDFSWTSLDIKPPPEEDNTQYPGLDFGSVLVKPSRPFCDSRIPLVVFIHGGPHSQFPAEWNSTTAGLVKLGCAVLMVNYRGSTGFGQDSILSLIGNIGSQDVKDVQRGVLAALWS-DETLDPKRVAVIGGSHGGFLCCHLVGQYPDFYKACAARNPVINAATLLGTSDIVDWRYTSAGFQFSYDSVPTAEVLAALLQKSPITHAVQIKAAVLLMLGGKDRRVSPHQGLELYKVLKTRASPVRLLWFPEDGHSLSRVDTQADCFVNTALWLQQ 697
BLAST of SMED30008513 vs. Ensembl Medaka
Match: zgc:136971 (acylamino-acid-releasing enzyme [Source:NCBI gene;Acc:101170588]) HSP 1 Score: 211.846 bits (538), Expect = 9.049e-57 Identity = 118/338 (34.91%), Postives = 181/338 (53.55%), Query Frame = -3
Query: 1117 KTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGEL--KSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKK---EGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTE 2115
KT ++ S S LL + ++V SSP+T P L +G L KS + + P + FD + PE D+ ++ + S+ + + + P+++ +HGGPHS F ++ A + +LG LMVNYRGS G+GQDSI SL+G IG DVKD + + +D ++ + GGSHGGFL HL Y D K+ A RNPVIN ++ +DI DW +T G ++ +D + E + + ++SP++ +IK +L+M+G D+RV P QGLELYK L+ R+ L +P+D H L+ V+ +D FVN W +
Sbjct: 422 KTDVEDLSAASRDCGSWKLLTIQRDLMVVCCSSPNTPPTLRVGFLPEKSEAMTWQTLQQPAMT---FDFSWTSLDIKPPPEEDNTQYPGLDFGSVLVKPSRPFCDSRIPLVVFIHGGPHSQFPAEWNSTTAGLVKLGCAVLMVNYRGSTGFGQDSILSLIGNIGSQDVKDVQRGVLAALWS-DETLDPKRVAVIGGSHGGFLCCHLVGQYPDFYKACAARNPVINAATLLGTSDIVDWRYTSAGFQFSYDSVPTAEVLAALLQKSPITHAVQIKAAVLLMLGGKDRRVSPHQGLELYKVLKTRASPVRLLWFPEDGHSLSRVDTQADCFVNTALWLQQ 755 HSP 2 Score: 141.354 bits (355), Expect = 5.561e-34 Identity = 108/400 (27.00%), Postives = 187/400 (46.75%), Query Frame = -3
Query: 2059 NITSVYKSYARYPNLNSAKFLNNADP----YIISSTWTVNDLVTSKTKNLNLNFYYEKDKN-------LILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFK--YKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEP-ITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETL 3216
I VY + +P SA P Y +++ W+ +DLV + + + D N ++ P + E++ +S +AI E SG + L+ +W K +NL ++IHG V+ + FG+ W + KL+Y+AE S + SA G + + Y +D+GE LT+ PV+C++D+ ++VL +S G P+ + F G+ + P+ LGL +C R LF ++ + +++ N S P+ SPDG +I+L+ GPH QC L + ++ + T ++DV+ P EF G+Y L+ CW+ DS ++ +SA + + ++D K+K V L++S+TV TL
Sbjct: 5 QIAGVYAEVSGFPTPVSAHVKVEELPEYRVYSVTADWSQSDLVRGSRLHYSQQWTLIADTNNHKTVRTVLPPGPFTPVSGELLR-AFSPIRGLKAIVRE------SGNQ---QLLEIWDCHGLRKCLNLTALNIHGRVYDDA-QFGSLSWSECEDKLLYVAEK------------SRNSSAGGESACREDRNVYCEDWGEALTSKSVPVVCVVDLHSGVVDVLQGVP--EDVSPGQALWAPDYQSVFFVGWYHEPFRLGLKFCSNRSSSLFVLDMEGR-CERLSGANLSVSCPRLSPDGSTLIFLQGRVFGPHHQCLSLQQLDLKSRKTSALIDVLNRPQAGEFAGVYET-LSPCCWSADSQRVVFSSACRNWKDLFVVDRKSKKV--LSLSDTVPLSTL 375
BLAST of SMED30008513 vs. Ensembl Medaka
Match: ENSORLT00000040051.1 (pep primary_assembly:ASM223467v1:1:37354481:37359289:-1 gene:ENSORLG00000026212.1 transcript:ENSORLT00000040051.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 45.4394 bits (106), Expect = 1.634e-12 Identity = 27/76 (35.53%), Postives = 40/76 (52.63%), Query Frame = 2
Query: 524 VKNAS*KFQRCMDKIISPLR-EFSGTYIGDIIVLSTSKKEHEKNLDLIREALKQENLSIKEEKSNIESISRQLDYL 748
+KNA FQR M+K++ LR YI D+I+ S S ++H K+L + L NL + +K RQL +L
Sbjct: 858 LKNAGATFQRLMEKVLGELRGRLCCVYIDDVIIFSPSAEQHLKDLQAVFGKLHSANLVLNLKKCTF--FQRQLRFL 931 HSP 2 Score: 36.5798 bits (83), Expect = 1.634e-12 Identity = 18/36 (50.00%), Postives = 22/36 (61.11%), Query Frame = 3
Query: 423 KKAYHQIPIALDYIPKTAVITHYGLYEYVKMTFALK 530
K Y Q+ +A D KTAVIT GLY++ M F LK
Sbjct: 824 KSGYWQVAMAEDSKAKTAVITPVGLYQFKSMPFGLK 859 HSP 3 Score: 30.4166 bits (67), Expect = 1.634e-12 Identity = 14/32 (43.75%), Postives = 19/32 (59.38%), Query Frame = 1
Query: 337 CGDYRGLNA-LRVDRYPLPRITGILDQLRGKK 429
C DYR LNA D YP+P I +L+ + G +
Sbjct: 785 CVDYRRLNAKTTADAYPMPLIHDLLESMHGAE 816
BLAST of SMED30008513 vs. Ensembl Medaka
Match: ENSORLT00000037906.1 (pep primary_assembly:ASM223467v1:16:28643737:28647831:-1 gene:ENSORLG00000027277.1 transcript:ENSORLT00000037906.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 43.5134 bits (101), Expect = 2.997e-12 Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 2
Query: 530 NAS*KFQRCMDKIISPLREFSGT-YIGDIIVLSTSKKEHEKNLDLIREALKQENLSIKEEK 709
NA FQR M+++ R S Y+ DII+ S+S ++H + LDL+ LKQ NL ++ +K
Sbjct: 480 NAPGTFQRLMERLFGDQRYQSLLLYLDDIIIFSSSFEQHLERLDLVFSRLKQYNLKVRLQK 540 HSP 2 Score: 36.1946 bits (82), Expect = 2.997e-12 Identity = 19/35 (54.29%), Postives = 22/35 (62.86%), Query Frame = 1
Query: 328 VQTCGDYRGLNA-LRVDRYPLPRITGILDQLRGKK 429
++ C DYR LNA R D +PLPRI LD L G K
Sbjct: 402 LRLCVDYRQLNANTRKDAFPLPRIDESLDALHGAK 436 HSP 3 Score: 31.9574 bits (71), Expect = 2.997e-12 Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3
Query: 429 AYHQIPIALDYIPKTAVITHYGLYEYVKMTFAL 527
YHQ+ +A KTA T +GL+E+ +M F L
Sbjct: 446 GYHQVGMAEKDRCKTAFCTPFGLFEFTRMPFGL 478
BLAST of SMED30008513 vs. Planmine SMEST
Match: SMESG000008541.1 (SMESG000008541.1) HSP 1 Score: 1474.14 bits (3815), Expect = 0.000e+0 Identity = 710/710 (100.00%), Postives = 710/710 (100.00%), Query Frame = -3
Query: 1102 MSVLKNITSVYKSYARYPNLNSAKFLNNADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYLDE 3231
MSVLKNITSVYKSYARYPNLNSAKFLNNADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYLDE
Sbjct: 1 MSVLKNITSVYKSYARYPNLNSAKFLNNADPYIISSTWTVNDLVTSKTKNLNLNFYYEKDKNLILKSPQVLMDEEIIMDVYSSSYKHRAIFLERKSNDSSGKESKTNLIHLWIDELFIKSINLNDMDIHGMVHKKSFNFGNFQWLKDGRKLVYIAESKKPKKLSFFQNVSHDQSAKGIEIGNQFKYKQDFGEQLTNVGNPVICILDVDEEQIEVLDSTTLCPGLSIGTVCCHPNGLGLAFTGYENMPYNLGLIYCIQRHCELFYYSLDSKEIKKIGDENKSQMSPKFSPDGRFIIWLETDASGPHMQCQRLMKYEIATGFTDVVVDVVKEPITEFTGLYNLGLTENCWTYDSNHILLTSAVKCQNKIILIDIKTKNVNILNVSETVNSETLLDLNDRFLLVKVSSPSTVPYLAIGELKSALLENEMRLVPLSSIYRFDNVLNCQILDHIPEFDHPKFGKKKYQSIFISSKKEGSCPMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVEASSDSFVNVLWWFTERYLDE 710
BLAST of SMED30008513 vs. Planmine SMEST
Match: SMESG000049836.1 (SMESG000049836.1) HSP 1 Score: 77.7962 bits (190), Expect = 2.572e-14 Identity = 59/215 (27.44%), Postives = 102/215 (47.44%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P++++ HGGP S+ + GF L V+YRGS GYG+ + L G D DC + ++ ++ E VD + + G S GG+ + + YSD ++ V +L S+ T + + + I DY ++ + ERSP++ IKC ++ G +DK VP Q ++Y+A++A+ + L +Y + H E
Sbjct: 404 PLLVLAHGGPTSSAKLSLNLNIQYYTSRGFAVLDVDYRGSTGYGKQYRNLLQHKWGLFDRIDCCEGAQYLVSE--HLVDRTMLAISGKSAGGY-TTLCSLTYSDIFRTGCSIYGVGDLKSLVEDTHKFESRYLDILIG---DYQQCQQLYH---ERSPINYPANIKCAMVFYQGDEDKVVPKEQSEKMYEAVKAQGIPCSLVMYKGERHGFRKAE 609
BLAST of SMED30008513 vs. Planmine SMEST
Match: SMESG000049836.1 (SMESG000049836.1) HSP 1 Score: 77.7962 bits (190), Expect = 2.603e-14 Identity = 59/215 (27.44%), Postives = 102/215 (47.44%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P++++ HGGP S+ + GF L V+YRGS GYG+ + L G D DC + ++ ++ E VD + + G S GG+ + + YSD ++ V +L S+ T + + + I DY ++ + ERSP++ IKC ++ G +DK VP Q ++Y+A++A+ + L +Y + H E
Sbjct: 406 PLLVLAHGGPTSSAKLSLNLNIQYYTSRGFAVLDVDYRGSTGYGKQYRNLLQHKWGLFDRIDCCEGAQYLVSE--HLVDRTMLAISGKSAGGY-TTLCSLTYSDIFRTGCSIYGVGDLKSLVEDTHKFESRYLDILIG---DYQQCQQLYH---ERSPINYPANIKCAMVFYQGDEDKVVPKEQSEKMYEAVKAQGIPCSLVMYKGERHGFRKAE 611
BLAST of SMED30008513 vs. Planmine SMEST
Match: SMESG000049836.1 (SMESG000049836.1) HSP 1 Score: 77.7962 bits (190), Expect = 2.787e-14 Identity = 59/215 (27.44%), Postives = 102/215 (47.44%), Query Frame = -3
Query: 1162 PMILMLHGGPHSAFYTDFTVFGAAVAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDRVKSAACRNPVINLISMTTATDIPDWNFTECGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLASVE 1806
P++++ HGGP S+ + GF L V+YRGS GYG+ + L G D DC + ++ ++ E VD + + G S GG+ + + YSD ++ V +L S+ T + + + I DY ++ + ERSP++ IKC ++ G +DK VP Q ++Y+A++A+ + L +Y + H E
Sbjct: 423 PLLVLAHGGPTSSAKLSLNLNIQYYTSRGFAVLDVDYRGSTGYGKQYRNLLQHKWGLFDRIDCCEGAQYLVSE--HLVDRTMLAISGKSAGGY-TTLCSLTYSDIFRTGCSIYGVGDLKSLVEDTHKFESRYLDILIG---DYQQCQQLYH---ERSPINYPANIKCAMVFYQGDEDKVVPKEQSEKMYEAVKAQGIPCSLVMYKGERHGFRKAE 628
BLAST of SMED30008513 vs. Planmine SMEST
Match: SMESG000018885.1 (SMESG000018885.1) HSP 1 Score: 61.6178 bits (148), Expect = 3.048e-9 Identity = 57/218 (26.15%), Postives = 102/218 (46.79%), Query Frame = -3
Query: 1171 PMILMLHGGPHS-AFYTDFTVFGAA--VAELGFHALMVNYRGSWGYGQDSIDSLLGYIGQMDVKDCIKASEDMIREMPSKVDGNKIFLYGGSHGGFLASHLAAHYSDR-VKSAACRNPVINLISMTTATDIPDWNFTE--CGIKWKFDYLLNKEDINTMWERSPLSLVDKIKCPILIMIGQDDKRVPPSQGLELYKALRARSVKTELYIYPKDCHPLA 1806
P++L ++ GP S A T FT A V+ L L V+ RG+ G++ ++ +G +++ D IKA+E+++ + ++ +I YG S+GGF H+ ++ ++ PV T+ D D +TE G+K K + DI+ + L IK L++ G D V +L ++L + ++ +YP + H LA
Sbjct: 591 PLLLSVYAGPSSQAVTTKFTFDWATYLVSSLNIINLQVDGRGTLNRGREFERAVYKNMGVIEIHDQIKATEEIVSKY-HFINKTQIGAYGWSYGGFAVGHILRTIDNKMIRCGVSVAPV-------TSFDYYDTAYTERYLGLKEKNPLAYQQTDISNFAKSFDLG----IKKKYLLIHGTKDDNVHFLNSAKLIRSLVEKGFDFDVMVYPDEAHTLA 796 The following BLAST results are available for this feature:
BLAST of SMED30008513 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30008513 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMED30008513 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of SMED30008513 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of SMED30008513 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30008513 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of SMED30008513 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30008513 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30008513 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30008513 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of SMED30008513 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008513 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30008513 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30008513 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008513 ID=SMED30008513|Name=SMED30008513|organism=Schmidtea mediterranea sexual|type=transcript|length=3344bpback to top protein sequence of SMED30008513-orf-1 >SMED30008513-orf-1 ID=SMED30008513-orf-1|Name=SMED30008513-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp HGQQHSNQQSWSWRFIVADVTCPILGDDFLVKHNIIVNYRNSENFKRYRTback to top protein sequence of SMED30008513-orf-2 >SMED30008513-orf-2 ID=SMED30008513-orf-2|Name=SMED30008513-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=711bp MSVLKNITSVYKSYARYPNLNSAKFLNNADPYIISSTWTVNDLVTSKTKNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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