SMED30008510
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008510 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30008510 aligns in the following genomic locations:
Homology
BLAST of SMED30008510 vs. Planmine SMEST
Match: SMESG000016753.1 (SMESG000016753.1) HSP 1 Score: 416.387 bits (1069), Expect = 1.377e-134 Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = -1 Query: 2768 YGYVPQPIIQHHYQHDFNKDCNIQRHFNQELPLNTLDFPVRHPRTRPGMPIRTPQFPNNGNVVNSHYFPVLSLQNFNNPYLIRSPFQHPKILTEKPINSIMDKTHNSIPNFNYRKSEKINKENLKFSNKHTPHKLVLNNQFFIATSFVNIKVCDKDSDDLIRHKMKYSGFSEKQIQSHLSSKRSNWDKMVNEIRMNCKNRKCY 3376 YGYVPQPIIQHHYQHDFNKDCNIQRHFNQELPLNTLDFPVRHPRTRPGMPIRTPQFPNNGNVVNSHYFPVLSLQNFNNPYLIRSPFQHPKILTEKPINSIMDKTHNSIPNFNYRKSEKINKENLKFSNKHTPHKLVLNNQFFIATSFVNIKVCDKDSDDLIRHKMKYSGFSEKQIQSHLSSKRSNWDKMVNEIRMNCKNRKCY Sbjct: 15 YGYVPQPIIQHHYQHDFNKDCNIQRHFNQELPLNTLDFPVRHPRTRPGMPIRTPQFPNNGNVVNSHYFPVLSLQNFNNPYLIRSPFQHPKILTEKPINSIMDKTHNSIPNFNYRKSEKINKENLKFSNKHTPHKLVLNNQFFIATSFVNIKVCDKDSDDLIRHKMKYSGFSEKQIQSHLSSKRSNWDKMVNEIRMNCKNRKCY 217
BLAST of SMED30008510 vs. Planmine SMEST
Match: SMESG000072682.1 (SMESG000072682.1) HSP 1 Score: 153.68 bits (387), Expect = 2.727e-41 Identity = 84/119 (70.59%), Postives = 99/119 (83.19%), Query Frame = 1 Query: 4039 NLKIAMEQRIREIDSIKNQLFQSRNLIKALKRECQLSNTKRQELEEENRSLNQKL--NRKWKQKVDELKSELATKWINNVLEDYWKKEAQRIKRKYCRRKNLNRAYKKIETYARKQYRK 4389 N+K+ +EQ+ +EIDSI NQ FQSRNLIKALK E QL+ KR+EL+EENRSLNQKL NR+W+Q VDELK ELA KW N+VL+DY KKEAQRIK+KY R K LNR YKK+ETYA KQY+K Sbjct: 366 NMKMTIEQQRQEIDSITNQWFQSRNLIKALKTEFQLAAIKRRELKEENRSLNQKLKLNREWEQNVDELKGELANKWANHVLKDYLKKEAQRIKKKYYRIKRLNREYKKLETYAVKQYQK 484 HSP 2 Score: 36.965 bits (84), Expect = 2.727e-41 Identity = 18/24 (75.00%), Postives = 19/24 (79.17%), Query Frame = 2 Query: 3917 KKDMYIKPIKIEV*NEDPIPTGCK 3988 KKD+ IKPIKIEV NED IP CK Sbjct: 325 KKDVRIKPIKIEVENEDQIPKECK 348
BLAST of SMED30008510 vs. Planmine SMEST
Match: SMESG000072682.1 (SMESG000072682.1) HSP 1 Score: 146.747 bits (369), Expect = 2.817e-39 Identity = 80/113 (70.80%), Postives = 94/113 (83.19%), Query Frame = 1 Query: 4039 NLKIAMEQRIREIDSIKNQLFQSRNLIKALKRECQLSNTKRQELEEENRSLNQKL--NRKWKQKVDELKSELATKWINNVLEDYWKKEAQRIKRKYCRRKNLNRAYKKIETYA 4371 N+K+ +EQ+ +EIDSI NQ FQSRNLIKALK E QL+ KR+EL+EENRSLNQKL NR+W+Q VDELK ELA KW N+VL+DY KKEAQRIK+KY R K LNR YKK+ETYA Sbjct: 366 NMKMTIEQQRQEIDSITNQWFQSRNLIKALKTEFQLAAIKRRELKEENRSLNQKLKLNREWEQNVDELKGELANKWANHVLKDYLKKEAQRIKKKYYRIKRLNREYKKLETYA 478 HSP 2 Score: 36.965 bits (84), Expect = 2.817e-39 Identity = 18/24 (75.00%), Postives = 19/24 (79.17%), Query Frame = 2 Query: 3917 KKDMYIKPIKIEV*NEDPIPTGCK 3988 KKD+ IKPIKIEV NED IP CK Sbjct: 325 KKDVRIKPIKIEVENEDQIPKECK 348
BLAST of SMED30008510 vs. Planmine SMEST
Match: SMESG000003311.1 (SMESG000003311.1) HSP 1 Score: 76.6406 bits (187), Expect = 1.509e-14 Identity = 49/100 (49.00%), Postives = 66/100 (66.00%), Query Frame = 1 Query: 4096 LFQSRNLIKALKRECQLSNTKRQELEEENRSLNQKLN--RKWKQKVDELKSELATKWINNVLEDYWKKEAQRIKRKYCRRKNLNRAYKKIETYARKQYRK 4389 + QSR L K LK EC + N K++ LEEEN L Q++ + ++KVDEL KW V E +WK +A+RI+RKY + K LNRAY+K+E YA KQY+K Sbjct: 179 VVQSRKLFKTLKEECLILN-KQKNLEEENELLRQQMEVCKGLEEKVDEL----VAKWTRYVQEKHWKVQAKRIQRKYRKTKRLNRAYRKLEGYAMKQYKK 273
BLAST of SMED30008510 vs. Planmine SMEST
Match: SMESG000009758.1 (SMESG000009758.1) HSP 1 Score: 70.0922 bits (170), Expect = 2.884e-13 Identity = 38/71 (53.52%), Postives = 56/71 (78.87%), Query Frame = 1 Query: 4036 RNLKIAMEQRIREIDSIKNQLFQSRNLIKALKRECQLSNTKRQELEEENRSLNQ--KLNRKWKQKVDELKS 4242 +N+KIAMEQ+I+EID + NQ FQSRNLIKALKRE + +R+E+E+EN+ L Q ++N+ W++ ++ELK Sbjct: 555 KNMKIAMEQQIQEIDCMTNQWFQSRNLIKALKRERDVLKRQRKEVEDENKWLKQNIEVNKGWEEGIEELKP 625 HSP 2 Score: 26.1794 bits (56), Expect = 2.884e-13 Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 2 Query: 3932 IKPIKIEV*NEDPIPTGCK 3988 IKP+K+EV NE PI CK Sbjct: 514 IKPLKMEVENEFPISMKCK 532 The following BLAST results are available for this feature:
BLAST of SMED30008510 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30008510 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30008510 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30008510 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30008510 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008510 ID=SMED30008510|Name=SMED30008510|organism=Schmidtea mediterranea sexual|type=transcript|length=4978bpback to top protein sequence of SMED30008510-orf-1 >SMED30008510-orf-1 ID=SMED30008510-orf-1|Name=SMED30008510-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp MEQRIREIDSIKNQLFQSRNLIKALKRECQLSNTKRQELEEENRSLNQKLback to top protein sequence of SMED30008510-orf-2 >SMED30008510-orf-2 ID=SMED30008510-orf-2|Name=SMED30008510-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=218bp MNFNINKINFFPNNYGYVPQPIIQHHYQHDFNKDCNIQRHFNQELPLNTLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|