DNA topoisomerase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008509 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 379.793 bits (974), Expect = 1.705e-118 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 367 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 976 HSP 2 Score: 62.3882 bits (150), Expect = 2.613e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 120 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 157
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 379.793 bits (974), Expect = 1.705e-118 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 367 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 976 HSP 2 Score: 62.3882 bits (150), Expect = 2.613e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 120 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 157
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 379.407 bits (973), Expect = 3.714e-118 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 392 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 1001 HSP 2 Score: 62.3882 bits (150), Expect = 2.635e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 182
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 379.407 bits (973), Expect = 3.714e-118 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 392 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 1001 HSP 2 Score: 62.3882 bits (150), Expect = 2.635e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 182
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 379.407 bits (973), Expect = 3.714e-118 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 392 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 1001 HSP 2 Score: 62.3882 bits (150), Expect = 2.635e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 182
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: top-3 (DNA topoisomerase 3 [Source:UniProtKB/Swiss-Prot;Acc:O61660]) HSP 1 Score: 226.868 bits (577), Expect = 6.602e-64 Identity = 117/270 (43.33%), Postives = 164/270 (60.74%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLK--QGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDE 926
FA +L G++P NG+KSD+AHPPIHPLK + L D+ +++EL+ RHFLAC+S+DA G ET V + + E F +GL + + YL VY Y+KW ++ +P Y GE F + I +T AP LTEADL+ LMDK+GIGTDATHAEHIEK+K R+Y+G G LIP LG+ LV+GY +G +S+PDLR+ E L+ IC+G +Q EVL+ + Y+ I+ + KI L + + +YLD+
Sbjct: 364 FANEVLQNGVNPRNGRKSDEAHPPIHPLKFTEKHQLQGDDWKVYELVVRHFLACVSQDAQGEETMVNLTVGT---------------------EKFHASGLRIRDMGYLKVYVYEKWGNRLLPTYTEGERFTDFELKIGDGKTQAPDFLTEADLISLMDKYGIGTDATHAEHIEKIKTREYIGVRPDGK--LIPSFLGLALVDGYDDMGFAMSKPDLRANLEIGLKEICDGRRQKQEVLDEQIGKYRAIFVESERKIGVLSQSLQRYLDK 610
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 121.324 bits (303), Expect = 3.854e-28 Identity = 89/261 (34.10%), Postives = 134/261 (51.34%), Query Frame = 3
Query: 126 ATRLLSEGI-HPHNG-KKSDQAHPPIHPLKQG-IGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPV----YGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKI 887
+++L++GI P +G K D HPPI P++ GL+ D +RI+E +++HF+A L K + T V + C G+ E F+L G +V+E + V P+ V++E V IG E + I ET+ P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L+P +LG LV GY V L P +R++ E L L+ +G L+V NH L ++ ++ T I
Sbjct: 369 VSQVLADGIKRPKSGVDKGD--HPPITPMRPNHGGLSGDMSRIYEYVSQHFVATLMKQCVYEVTTVKMSC-------------GD--------EQFTLQGKNVVEPGFTAVMPWLS-VEEECSVPKSLQQIGAKIELQNAEIQARETTPPGYLTEAELITLMEKHGIGTDASIPVHINTITQRNYV--TVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNI 603
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 120.939 bits (302), Expect = 3.943e-28 Identity = 90/263 (34.22%), Postives = 134/263 (50.95%), Query Frame = 3
Query: 126 ATRLLSEGI-HPHNG-KKSDQAHPPIHPLKQG-IGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKE----VP--VYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKI 887
+++L++GI P +G K D HPPI P++ GL+ D +RI+E +++HF+A L K + T V + C G+ E F+L G +V+E + V P W+ E VP + IG E + I ET+ P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L+P +LG LV GY V L P +R++ E L L+ +G L+V NH L ++ ++ T I
Sbjct: 233 VSQVLADGIKRPKSGVDKGD--HPPITPMRPNHGGLSGDMSRIYEYVSQHFVATLMKQCVYEVTTVKMSC-------------GD--------EQFTLQGKNVVEPGFTAVMP---WLSVEEECSVPKSLQQIGAKIELQNAEIQARETTPPGYLTEAELITLMEKHGIGTDASIPVHINTITQRNYV--TVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNI 467
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 65.855 bits (159), Expect = 3.233e-11 Identity = 38/100 (38.00%), Postives = 54/100 (54.00%), Query Frame = 3
Query: 588 MDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKI 887
M+KHGIGTDA+ HI + R+YV T L+P +LG LV GY V L P +R++ E L L+ +G L+V NH L ++ ++ T I
Sbjct: 1 MEKHGIGTDASIPVHINTITQRNYV--TVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNI 98
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3alpha (gene:FBgn0040268 transcript:FBtr0081246) HSP 1 Score: 302.368 bits (773), Expect = 1.412e-88 Identity = 165/411 (40.15%), Postives = 226/411 (54.99%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDE--NDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNV--WFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSK 1343
FA R++ G +P NG KSDQAHPPIHP K L +EAR++EL+ RHFLAC+SKDA+G ET V ID GE F+ GL + ERNYLDVY YDKW K++ Y G+ FEPT VS+ + T+AP LLTEADL+ LM+KHGIGTDATHAEHI +K R Y+G G FL+PG +GMGL EGY + L++P LR++FE DL+LIC G K P VL + YK+ Y+ KI + ++ ++E P + + A ++ + +++ CP C L K KKN W++GC N+P CK+A+WLP + + E TC + + +K + + + +F + CL CD F NI + K
Sbjct: 386 FAQRVIEWGPNPRNGNKSDQAHPPIHPTKLAENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIA---------------------GEKFTANGLVIHERNYLDVYVYDKWSAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGVLDKG--FLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADGSAPSHGIIQSIFQCPKCNEAPLALKPKKNQQGWYIGCNNFPDCKNAVWLPTECKDASVLD----ECCPTCGDGYRM----------LKFRLSTPYYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVK 759 HSP 2 Score: 78.1814 bits (191), Expect = 1.690e-14 Identity = 63/228 (27.63%), Postives = 84/228 (36.84%), Query Frame = 3
Query: 1548 CGQIAATRKVSKEGPNKNKFFYTCSS-SKCQMFAWVES---------------------------------------GDVRNQNPHCSSTVMNSANTPQSSNNTRS-----DVNCNCGKPVNLLTVRKENQNKGRQFYSC----SQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPP----PAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
C Q A V K GPN + +Y C +C F W + G ++ S+ + T Q NT + +V CNCG+ + LTVRK+ N+GR FY+C C FF+W DE G +ST ++N PP P I PK+ R C LCR+ GHT KCP K
Sbjct: 1038 CQQPARQNTVRKNGPNLGRLYYKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQSNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLASQLTVRKDGPNQGRPFYACPTREKSCGFFKWGDEDQNQGASSTSWG-------------------SANRNPPGRSQPTAITSDGPKT-RRCGLCRKEGHTRNKCPRK 1245 HSP 3 Score: 65.855 bits (159), Expect = 1.141e-10 Identity = 28/38 (73.68%), Postives = 31/38 (81.58%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+D C AIKPNI+V RA FSEIT
Sbjct: 137 IWTDCDREGENIGYEIIDVCRAIKPNISVYRATFSEIT 174 HSP 4 Score: 51.2174 bits (121), Expect = 4.118e-6 Identity = 33/88 (37.50%), Postives = 46/88 (52.27%), Query Frame = 3
Query: 1713 NTPQSSNNTRSDVNC-NCGKPVNLLTVRKENQNKGRQFYSC---SQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTS 1964
+TP SS V C C +P TVRK N GR +Y C +C+FFQW+DEP +A +K + + QS +G N+ + L S
Sbjct: 1021 STPPSSVPESETVLCTGCQQPARQNTVRKNGPNLGRLYYKCPKPDECNFFQWADEPP--SSAKSKNSTGSAPQSTTSWGSNRVVTLPS 1106
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3beta (gene:FBgn0026015 transcript:FBtr0340028) HSP 1 Score: 100.138 bits (248), Expect = 2.203e-21 Identity = 74/248 (29.84%), Postives = 116/248 (46.77%), Query Frame = 3
Query: 126 ATRLLSEGIHPHNGKKSDQAHPPIHPLKQG--IGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIG-EGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYK 857
A +L + P GK + HPPI P+K G + D R++E I RHF+ +S+D T + +G E FS T +++ + V + + D+ P + G V + +S+T P LTE++L+ LM++HGIGTDA+ HI + R+YV + G L+P LG+ LV GY + L P +R++ E L LI G+ +VL H + +K
Sbjct: 363 ARSILGDIQTPRKGKDAGD-HPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKL----------------------SVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYV-HIENGRK-LMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFK 585
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3beta (gene:FBgn0026015 transcript:FBtr0070930) HSP 1 Score: 100.138 bits (248), Expect = 2.203e-21 Identity = 74/248 (29.84%), Postives = 116/248 (46.77%), Query Frame = 3
Query: 126 ATRLLSEGIHPHNGKKSDQAHPPIHPLKQG--IGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIG-EGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYK 857
A +L + P GK + HPPI P+K G + D R++E I RHF+ +S+D T + +G E FS T +++ + V + + D+ P + G V + +S+T P LTE++L+ LM++HGIGTDA+ HI + R+YV + G L+P LG+ LV GY + L P +R++ E L LI G+ +VL H + +K
Sbjct: 363 ARSILGDIQTPRKGKDAGD-HPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKL----------------------SVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYV-HIENGRK-LMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFK 585
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 378.252 bits (970), Expect = 6.461e-118 Identity = 251/672 (37.35%), Postives = 337/672 (50.15%), Query Frame = 3
Query: 123 FATRLL-SEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWV----ESG-DVRNQNPHCSSTVM------NSANTPQSSNNTR------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCP 2066
FA R+L S G P NG KSDQAHPPIHP K GL +E R++E I RHFLAC SKDA G E V ID E FS +GL+++ RNYL+VYPYDKW +K +P Y F+PT++ + + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T FL PG+LGMGLVEGY+ +G +S+PDLR++ EADL+L+ G K VL++++ YK ++ + K KL E ++ YL A E + P VR CP C M+L +K L C YPACK A+W PDTV L S++ TC H H + S+ F+G I CD + E LN+++ + + + + ++ S+Q S PP + ++N I + Q + Q ++ VC+CGQ A V KEGPN+ + FY C++ C+ F W E G RN+ P S V N Q N R + CNC +P TV KE NKGR F++C QC FFQW++E A + N + NK+ A T++V PP A K R+C +C +PGHT CP
Sbjct: 390 FAQRILESGGPTPRNGNKSDQAHPPIHPTKYTNGLQGNEKRLYEFIVRHFLACCSKDAQGQEVTVEIDIAE---------------------ERFSASGLTIIARNYLEVYPYDKWYNKVIPSYSPNTTFQPTAIEMVEGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTA-DQRFL-PGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKNKASVLSYHVAKYKAVFIESVRKAKKLDEALSNYLGPAQEIAEQEQVELEIPLP-----VRKCPQCNRDMVLKKKKDSTGMLLSCLGYPACKAAVWFPDTV----LEVSRDESICPTCRPHPVHMLKFKFKRGSLPPMMPLEFVGCIG-----------GCDDTLREVLNLKYLRGRDAESAPQANNSSRSSQHHIHRTSSAPPPSR-VEN--IRPPAPRPRADPGLQPPPSTQGGAI----VCNCGQDALLLTVRKEGPNQGRQFYKCNTGDCKFFLWADQPPEQGVPERNRGP-LSQNVQPPRPSNGFGNVSQQRQNHRGSGGDEGETMCNCNEPSVTRTVMKEGPNKGRMFHTCGKPRDQQCGFFQWANENVAPATAFSGGNGGKP-------SGNKK-ASTTSVKPPTA-------KRQRTCGICHEPGHTRVTCP 995 HSP 2 Score: 65.4698 bits (158), Expect = 1.924e-10 Identity = 27/38 (71.05%), Postives = 31/38 (81.58%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+D C A+KPNI V RA+FSEIT
Sbjct: 142 VWTDCDREGENIGFEIIDVCKAVKPNIQVFRARFSEIT 179
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3b (DNA topoisomerase III beta [Source:ZFIN;Acc:ZDB-GENE-070705-37]) HSP 1 Score: 121.709 bits (304), Expect = 5.156e-28 Identity = 86/248 (34.68%), Postives = 125/248 (50.40%), Query Frame = 3
Query: 135 LLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKE-VPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIY 866
LLSEGI+ P G + HPPI P++ L D R+++ ITRHF+A +S D K L IS + + EGF+ +G +++ Y +V P+ +E +PV GE+F + + + +T+ P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K +
Sbjct: 370 LLSEGINRPRKGADAGD-HPPITPMRAASENELGSDGWRLYDYITRHFIATVSPDC-----KYLQTTISFSIAT----------------EGFTCSGKTLISAGYTEVMPWQGIAQEEALPVCERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYV--TVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKF 593
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3b (DNA topoisomerase III beta [Source:ZFIN;Acc:ZDB-GENE-070705-37]) HSP 1 Score: 121.709 bits (304), Expect = 5.156e-28 Identity = 86/248 (34.68%), Postives = 125/248 (50.40%), Query Frame = 3
Query: 135 LLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKE-VPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIY 866
LLSEGI+ P G + HPPI P++ L D R+++ ITRHF+A +S D K L IS + + EGF+ +G +++ Y +V P+ +E +PV GE+F + + + +T+ P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K +
Sbjct: 370 LLSEGINRPRKGADAGD-HPPITPMRAASENELGSDGWRLYDYITRHFIATVSPDC-----KYLQTTISFSIAT----------------EGFTCSGKTLISAGYTEVMPWQGIAQEEALPVCERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYV--TVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKF 593
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: BX005237.1 (DNA topoisomerase III beta [Source:NCBI gene;Acc:793414]) HSP 1 Score: 121.324 bits (303), Expect = 5.213e-28 Identity = 86/248 (34.68%), Postives = 125/248 (50.40%), Query Frame = 3
Query: 135 LLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKE-VPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIY 866
LLSEGI+ P G + HPPI P++ L D R+++ ITRHF+A +S D K L IS + + EGF+ +G +++ Y +V P+ +E +PV GE+F + + + +T+ P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K +
Sbjct: 327 LLSEGINRPRKGADAGD-HPPITPMRAASENELGSDGWRLYDYITRHFIATVSPDC-----KYLQTTISFSIAT----------------EGFTCSGKTLISAGYTEVMPWQGIAQEEALPVCERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYV--TVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKF 550
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: neil3 (nei-like DNA glycosylase 3 [Source:ZFIN;Acc:ZDB-GENE-041114-18]) HSP 1 Score: 53.9138 bits (128), Expect = 5.534e-7 Identity = 49/180 (27.22%), Postives = 73/180 (40.56%), Query Frame = 3
Query: 1380 PSNQAQPKVMKSTPPFKKEIK------NQPISQSSFDFPTSSFR----QELENLQDTSVKSTPVCHCGQIAAT-RKVSKEGPNKNKFFYTCS---SSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSC-----SQCSFFQWSD 1862
PSN K P+K+E N+ + ++ F + + + + D S+ +TP C T R V+KEG NK + FYTCS ++C F W + + P C+ GK + TV K N GR FY+C QC+FFQW++
Sbjct: 406 PSNGVSGMQFKGNEPYKRESPTDHSQPNKRMKTTNGTFSGGNIKHISSRGTQQSNDASLPTTPCCKSHHRPCTQRVVTKEGENKGRQFYTCSLPRETQCNFFEWAD------------------LHFPM----------CHHGKRTVMKTVLKLGPNNGRNFYTCPVKMGKQCNFFQWAE 557
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 387.497 bits (994), Expect = 2.473e-121 Identity = 245/686 (35.71%), Postives = 335/686 (48.83%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---------HTSKPSSNFQ-NSLSTIPSNQA-----QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAW---VESGDVRNQNPHCSSTVMNSANTPQSSNNTR-----------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NG K+DQAHPPIHP K L +E RI+E I RHFLAC S+DA G ET V ID + E F GL ++ RNYLDVYPYDKW K +PVY +G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ E+DL+LIC G K VL + YK+++ K KL E +++Y G P + T VR CP C ++++ + +FL C YP CK ++W P V V S++ TC H + + + S+ F+G I CD++ E L++++ + S++ Q N S N QP+ PP K ++ ++ + P+SS E+ VC+CG A V KEGPN+ + FY C+ C F W +SG Q H + N+ + P++S++ R C C +P TV+K+ NKGRQF++CS QC FFQW+DE P G+ +P FG + + +S P K RSC +C QPGH CP +
Sbjct: 380 FAQRILERGGPTPRNGNKTDQAHPPIHPTKYTNSLQGNEQRIYEFIVRHFLACCSQDAQGQETTVEIDIAA---------------------ERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQPSTVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLT-PEQRFL-PGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYF-----GQAAQPVPQEEIYADTPLPVRKCPQCNKDMVLKTKRDGGFFLSCVGYPGCKSSVWFPSFVLEV----SRDESICGTCKPHPVYKLKMKFKRGSVPPLMPLEFVGCIG-----------GCDETLREILDLKYLHGGNRAPQAAPRESNHLQVNQTSNRMGNSGDGRGRQPQAKTLKPPVAKPSASRGVAPT----PSSSESNEV------------VCNCGVAAVQLTVRKEGPNQGRPFYKCNGGACNFFLWADQADSGSPNAQGGHSTGRPSNAFSNPRASDSFRPAGGGSEPGSEGQAVCMCNQPAVTRTVQKDGANKGRQFHTCSKPREQQCGFFQWADENVPPGSG-----------APRGFGKKNKRSESSFSGP--------VAKKQRSCGICHQPGHNRTNCPQNR 987 HSP 2 Score: 60.8474 bits (146), Expect = 6.691e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C ++KPN+ V RA+FSEIT
Sbjct: 133 IWTDCDREGENIGFEIIHVCKSVKPNLQVFRARFSEIT 170
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 387.882 bits (995), Expect = 2.688e-121 Identity = 245/686 (35.71%), Postives = 337/686 (49.13%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---------HTSKPSSNFQ-NSLSTIPSNQA-----QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAW---VESGDVRNQNPHCSSTVMNSANTPQSSNNTR-----------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NG K+DQAHPPIHP K L +E RI+E I RHFLAC S+DA G ET V ID + E F GL ++ RNYLDVYPYDKW K +PVY +G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ E+DL+LIC G K VL + YK+++ K KL E +++Y +A P + T VR CP C ++++ + +FL C YP CK ++W P V V S++ TC H + + + S+ F+G I CD++ E L++++ + S++ Q N S N QP+ PP K ++ ++ + P+SS E+ VC+CG A V KEGPN+ + FY C+ C F W +SG Q H + N+ + P++S++ R C C +P TV+K+ NKGRQF++CS QC FFQW+DE P G ++ +P FG + + +S P K RSC +C QPGH CP +
Sbjct: 397 FAQRILERGGPTPRNGNKTDQAHPPIHPTKYTNSLQGNEQRIYEFIVRHFLACCSQDAQGQETTVEIDIAA---------------------ERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQPSTVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLT-PEQRFL-PGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYFGQAA-----QPVPQEEIYADTPLPVRKCPQCNKDMVLKTKRDGGFFLSCVGYPGCKSSVWFPSFVLEV----SRDESICGTCKPHPVYKLKMKFKRGSVPPLMPLEFVGCIG-----------GCDETLREILDLKYLHGGNRAPQAAPRESNHLQVNQTSNRMGNSGDGRGRQPQAKTLKPPVAKPSASRGVAPT----PSSSESNEV------------VCNCGVAAVQLTVRKEGPNQGRPFYKCNGGACNFFLWADQADSGSPNAQGGHSTGRPSNAFSNPRASDSFRPAGGGSEPGSEGQAVCMCNQPAVTRTVQKDGANKGRQFHTCSKPREQQCGFFQWADENVPPGKEASNPG------APRGFGKKNKRSESSFSGP--------VAKKQRSCGICHQPGHNRTNCPQNR 1009 HSP 2 Score: 60.8474 bits (146), Expect = 6.738e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C ++KPN+ V RA+FSEIT
Sbjct: 150 IWTDCDREGENIGFEIIHVCKSVKPNLQVFRARFSEIT 187
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 386.726 bits (992), Expect = 2.825e-121 Identity = 245/686 (35.71%), Postives = 336/686 (48.98%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---------HTSKPSSNFQ-NSLSTIPSNQA-----QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAW---VESGDVRNQNPHCSSTVMNSANTPQSSNNTR-----------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NG K+DQAHPPIHP K L +E RI+E I RHFLAC S+DA G ET V ID + E F GL ++ RNYLDVYPYDKW K +PVY +G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ E+DL+LIC G K VL + YK+++ K KL E +++Y +A P + T VR CP C ++++ + +FL C YP CK ++W P V V S++ TC H + + + S+ F+G I CD++ E L++++ + S++ Q N S N QP+ PP K ++ ++ + P+SS E+ VC+CG A V KEGPN+ + FY C+ C F W +SG Q H + N+ + P++S++ R C C +P TV+K+ NKGRQF++CS QC FFQW+DE P G+ +P FG + + +S P K RSC +C QPGH CP +
Sbjct: 368 FAQRILERGGPTPRNGNKTDQAHPPIHPTKYTNSLQGNEQRIYEFIVRHFLACCSQDAQGQETTVEIDIAA---------------------ERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQPSTVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLT-PEQRFL-PGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYFGQAA-----QPVPQEEIYADTPLPVRKCPQCNKDMVLKTKRDGGFFLSCVGYPGCKSSVWFPSFVLEV----SRDESICGTCKPHPVYKLKMKFKRGSVPPLMPLEFVGCIG-----------GCDETLREILDLKYLHGGNRAPQAAPRESNHLQVNQTSNRMGNSGDGRGRQPQAKTLKPPVAKPSASRGVAPT----PSSSESNEV------------VCNCGVAAVQLTVRKEGPNQGRPFYKCNGGACNFFLWADQADSGSPNAQGGHSTGRPSNAFSNPRASDSFRPAGGGSEPGSEGQAVCMCNQPAVTRTVQKDGANKGRQFHTCSKPREQQCGFFQWADENVPPGSG-----------APRGFGKKNKRSESSFSGP--------VAKKQRSCGICHQPGHNRTNCPQNR 975 HSP 2 Score: 60.8474 bits (146), Expect = 6.665e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C ++KPN+ V RA+FSEIT
Sbjct: 121 IWTDCDREGENIGFEIIHVCKSVKPNLQVFRARFSEIT 158
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 385.571 bits (989), Expect = 1.008e-120 Identity = 246/690 (35.65%), Postives = 341/690 (49.42%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---------HTSKPSSNFQ-NSLSTIPSNQA-----QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAW---VESGDVRNQNPHCSSTVMNSANTPQSSNNTR-----------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTK----LNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NG K+DQAHPPIHP K L +E RI+E I RHFLAC S+DA G ET V ID + E F GL ++ RNYLDVYPYDKW K +PVY +G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ E+DL+LIC G K VL + YK+++ K KL E +++Y +A P + T VR CP C ++++ + +FL C YP CK ++W P V V S++ TC H + + + S+ F+G I CD++ E L++++ + S++ Q N S N QP+ PP K ++ ++ + P+SS E+ VC+CG A V KEGPN+ + FY C+ C F W +SG Q H + N+ + P++S++ R C C +P TV+K+ NKGRQF++CS QC FFQW+DE P G ++ +++ S +P FG + + +S P K RSC +C QPGH CP +
Sbjct: 362 FAQRILERGGPTPRNGNKTDQAHPPIHPTKYTNSLQGNEQRIYEFIVRHFLACCSQDAQGQETTVEIDIAA---------------------ERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQPSTVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLT-PEQRFL-PGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYFGQAA-----QPVPQEEIYADTPLPVRKCPQCNKDMVLKTKRDGGFFLSCVGYPGCKSSVWFPSFVLEV----SRDESICGTCKPHPVYKLKMKFKRGSVPPLMPLEFVGCIG-----------GCDETLREILDLKYLHGGNRAPQAAPRESNHLQVNQTSNRMGNSGDGRGRQPQAKTLKPPVAKPSASRGVAPT----PSSSESNEV------------VCNCGVAAVQLTVRKEGPNQGRPFYKCNGGACNFFLWADQADSGSPNAQGGHSTGRPSNAFSNPRASDSFRPAGGGSEPGSEGQAVCMCNQPAVTRTVQKDGANKGRQFHTCSKPREQQCGFFQWADENVPPGKEASNPGDWVHVGGS-GAPRGFGKKNKRSESSFSGP--------VAKKQRSCGICHQPGHNRTNCPQNR 983 HSP 2 Score: 60.8474 bits (146), Expect = 6.682e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C ++KPN+ V RA+FSEIT
Sbjct: 115 IWTDCDREGENIGFEIIHVCKSVKPNLQVFRARFSEIT 152
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 383.645 bits (984), Expect = 7.313e-120 Identity = 244/686 (35.57%), Postives = 335/686 (48.83%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---------HTSKPSSNFQ-NSLSTIPSNQA-----QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAW---VESGDVRNQNPHCSSTVMNSANTPQSSNNTR-----------SDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NG K+DQAHPPIHP K L +E RI+E I RHFLAC S+DA G ET V ID + E F GL ++ RNYLDVYPYDKW K +PVY +G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ E+DL+LIC G K VL + YK+++ K KL E +++Y +A P + T VR CP C ++++ + +FL C YP CK ++W P V V S++ TC H + + + S+ F+G I CD++ E L++++ + S++ Q N S N QP+ PP K ++ ++ + P+SS E+ VC+CG A V KEGPN+ + FY C+ C F W +SG Q H + N+ + P++S++ R C C +P TV+K+ NKGRQF++CS QC FFQW+DE P G ++ FG + + +S P K RSC +C QPGH CP +
Sbjct: 387 FAQRILERGGPTPRNGNKTDQAHPPIHPTKYTNSLQGNEQRIYEFIVRHFLACCSQDAQGQETTVEIDIAA---------------------ERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQPSTVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLT-PEQRFL-PGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYFGQAA-----QPVPQEEIYADTPLPVRKCPQCNKDMVLKTKRDGGFFLSCVGYPGCKSSVWFPSFVLEV----SRDESICGTCKPHPVYKLKMKFKRGSVPPLMPLEFVGCIG-----------GCDETLREILDLKYLHGGNRAPQAAPRESNHLQVNQTSNRMGNSGDGRGRQPQAKTLKPPVAKPSASRGVAPT----PSSSESNEV------------VCNCGVAAVQLTVRKEGPNQGRPFYKCNGGACNFFLWADQADSGSPNAQGGHSTGRPSNAFSNPRASDSFRPAGGGSEPGSEGQAVCMCNQPAVTRTVQKDGANKGRQFHTCSKPREQQCGFFQWADENVPPGKEASNPG----------FGKKNKRSESSFSGP--------VAKKQRSCGICHQPGHNRTNCPQNR 995 HSP 2 Score: 60.8474 bits (146), Expect = 6.708e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C ++KPN+ V RA+FSEIT
Sbjct: 140 IWTDCDREGENIGFEIIHVCKSVKPNLQVFRARFSEIT 177
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3a (topoisomerase (DNA) III alpha [Source:MGI Symbol;Acc:MGI:1197527]) HSP 1 Score: 383.259 bits (983), Expect = 8.670e-120 Identity = 246/688 (35.76%), Postives = 332/688 (48.26%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF-----HTSKPSSNFQ-----NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVN------------------CNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIAL--TSNVWPPPAHINKGKP-KSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K GL D+ R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K +VL + YK+++ K KL E +++YL G+ + A + P E VR CP C ++++ K ++L C +P C+ A+W PD+V L S++ C + L + S+ F+G I CD++ E +RF S+PS + Q N + + N +QP V + T P K ++Q+ PT++ ++ C+CG+ A V K+GPN+ + FY CS+ C F W +S T +++ P SS S V C CG+P TV+K+ NKGRQF++C+ QC FFQW DE G SF +P G + ++ P + G K R C LC QPGHT CP +
Sbjct: 392 FAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSD--KRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYL-----GERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSV----LEASRDNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIG-----------GCDETLKEIFGLRFPRALPRASQPSGHLQASQALNRMDSSQHNLSQPLVNRHTRPSKT------VAQALLP-PTTA-----------GESNSVTCNCGREAVLLTVRKQGPNQGRHFYKCSNGDCNFFLWADSSHSTG-----GGTPTSASGPPGSSVGCPSSVGSHMDGFGSLGSDSDGGTPCLCGQPAVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPG----------SFAAPAWPGGRGKAQRPEAASKRPRAGSSDAGSTVKKPRKCSLCHQPGHTRTFCPQNR 1003 HSP 2 Score: 62.003 bits (149), Expect = 2.058e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEIT 182
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3a (topoisomerase (DNA) III alpha [Source:MGI Symbol;Acc:MGI:1197527]) HSP 1 Score: 373.244 bits (957), Expect = 2.159e-116 Identity = 229/623 (36.76%), Postives = 310/623 (49.76%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF-----HTSKPSSNFQ-----NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVN------------------CNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNAS 1889
FA +L G P NG KSDQAHPPIHP K GL D+ R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K +VL + YK+++ K KL E +++YL G+ + A + P E VR CP C ++++ K ++L C +P C+ A+W PD+V L S++ C + L + S+ F+G I CD++ E +RF S+PS + Q N + + N +QP V + T P K ++Q+ PT++ ++ C+CG+ A V K+GPN+ + FY CS+ C F W +S T +++ P SS S V C CG+P TV+K+ NKGRQF++C+ QC FFQW DE G A+
Sbjct: 392 FAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSD--KRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYL-----GERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSV----LEASRDNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIG-----------GCDETLKEIFGLRFPRALPRASQPSGHLQASQALNRMDSSQHNLSQPLVNRHTRPSKT------VAQALLP-PTTA-----------GESNSVTCNCGREAVLLTVRKQGPNQGRHFYKCSNGDCNFFLWADSSHSTG-----GGTPTSASGPPGSSVGCPSSVGSHMDGFGSLGSDSDGGTPCLCGQPAVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPGEAA 948 HSP 2 Score: 62.003 bits (149), Expect = 2.024e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEIT 182
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3a (topoisomerase (DNA) III alpha [Source:MGI Symbol;Acc:MGI:1197527]) HSP 1 Score: 303.138 bits (775), Expect = 1.279e-91 Identity = 168/411 (40.88%), Postives = 221/411 (53.77%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSS 1352
FA +L G P NG KSDQAHPPIHP K GL D+ R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K +VL + YK+++ K KL E +++YL G+ + A + P E VR CP C ++++ K ++L C +P C+ A+W PD+V L S++ C + L + S+ F+G I CD++ E +RF + P +
Sbjct: 367 FAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSD--KRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYL-----GERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSV----LEASRDNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIG-----------GCDETLKEIFGLRFPRALPRA 734 HSP 2 Score: 62.003 bits (149), Expect = 1.860e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 120 IWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEIT 157
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3a (topoisomerase (DNA) III alpha [Source:MGI Symbol;Acc:MGI:1197527]) HSP 1 Score: 284.648 bits (727), Expect = 1.111e-85 Identity = 149/323 (46.13%), Postives = 189/323 (58.51%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACK 1088
FA +L G P NG KSDQAHPPIHP K GL D+ R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K +VL + YK+++ K KL E +++YL G+ + A + P E VR CP C ++++ K ++L C +P C+
Sbjct: 367 FAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSD--KRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYL-----GERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECR 661 HSP 2 Score: 60.077 bits (144), Expect = 7.332e-9 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 120 IWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEIT 157
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3b (topoisomerase (DNA) III beta [Source:MGI Symbol;Acc:MGI:1333803]) HSP 1 Score: 116.316 bits (290), Expect = 7.207e-27 Identity = 86/250 (34.40%), Postives = 124/250 (49.60%), Query Frame = 3
Query: 132 RLLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIG-EGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIY 866
+LL+EGI+ P G + HPPI P+K L D R++E ITRHF+A +S H+ K L IS IG E F+ G +V+ + ++ P+ +++ +P G+ F V + + +TS P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K +
Sbjct: 164 QLLAEGINRPRKGHDAGD-HPPITPMKSATEAELGGDAWRLYEYITRHFIATVS-----HDCKYLQSTIS-----------------FRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYV--TVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKF 388
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|O70157|TOP3A_MOUSE (DNA topoisomerase 3-alpha OS=Mus musculus OX=10090 GN=Top3a PE=2 SV=1) HSP 1 Score: 383.259 bits (983), Expect = 6.065e-119 Identity = 246/688 (35.76%), Postives = 332/688 (48.26%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF-----HTSKPSSNFQ-----NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVN------------------CNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIAL--TSNVWPPPAHINKGKP-KSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K GL D+ R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K +VL + YK+++ K KL E +++YL G+ + A + P E VR CP C ++++ K ++L C +P C+ A+W PD+V L S++ C + L + S+ F+G I CD++ E +RF S+PS + Q N + + N +QP V + T P K ++Q+ PT++ ++ C+CG+ A V K+GPN+ + FY CS+ C F W +S T +++ P SS S V C CG+P TV+K+ NKGRQF++C+ QC FFQW DE G SF +P G + ++ P + G K R C LC QPGHT CP +
Sbjct: 392 FAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSD--KRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYL-----GERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSV----LEASRDNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIG-----------GCDETLKEIFGLRFPRALPRASQPSGHLQASQALNRMDSSQHNLSQPLVNRHTRPSKT------VAQALLP-PTTA-----------GESNSVTCNCGREAVLLTVRKQGPNQGRHFYKCSNGDCNFFLWADSSHSTG-----GGTPTSASGPPGSSVGCPSSVGSHMDGFGSLGSDSDGGTPCLCGQPAVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPG----------SFAAPAWPGGRGKAQRPEAASKRPRAGSSDAGSTVKKPRKCSLCHQPGHTRTFCPQNR 1003 HSP 2 Score: 62.003 bits (149), Expect = 1.440e-8 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEIT 182
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|Q13472|TOP3A_HUMAN (DNA topoisomerase 3-alpha OS=Homo sapiens OX=9606 GN=TOP3A PE=1 SV=1) HSP 1 Score: 379.407 bits (973), Expect = 1.784e-117 Identity = 246/679 (36.23%), Postives = 333/679 (49.04%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSS--TVMNSANTPQS-----------SNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE---PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC+G K VL + YK+++ K KL E +A+Y G+ + A + P E +R CP C ++++ K ++L C +P C+ A+WLPD+V L S+++ C H + L + S+ F+ C+ CD + E L++RF S+PS Q +NQ+ ++ S P QP + S + + Q L ++ C+CGQ A V KEGPN+ + F+ C+ C F W +S + P + + S P + + S +C C +P TV+K+ NKGRQF++C+ QC FFQW DE P G S + R+ +S E + A +S++ K R C LC QPGHT CP +
Sbjct: 392 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYF-----GNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSV----LEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFV------------CCIGGCDDTLREILDLRFSGGPPRASQPSGRLQ-------ANQSLNRMDNSQHP-------QP-ADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLES--EARSKRPRASSSDM--------GSTAKKPRKCSLCHQPGHTRPFCPQNR 1001 HSP 2 Score: 62.3882 bits (150), Expect = 1.266e-8 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 145 IWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEIT 182
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|Q9NG98|TOP3A_DROME (DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 SV=2) HSP 1 Score: 302.368 bits (773), Expect = 1.415e-87 Identity = 165/411 (40.15%), Postives = 226/411 (54.99%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDE--NDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNV--WFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSK 1343
FA R++ G +P NG KSDQAHPPIHP K L +EAR++EL+ RHFLAC+SKDA+G ET V ID GE F+ GL + ERNYLDVY YDKW K++ Y G+ FEPT VS+ + T+AP LLTEADL+ LM+KHGIGTDATHAEHI +K R Y+G G FL+PG +GMGL EGY + L++P LR++FE DL+LIC G K P VL + YK+ Y+ KI + ++ ++E P + + A ++ + +++ CP C L K KKN W++GC N+P CK+A+WLP + + E TC + + +K + + + +F + CL CD F NI + K
Sbjct: 386 FAQRVIEWGPNPRNGNKSDQAHPPIHPTKLAENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIA---------------------GEKFTANGLVIHERNYLDVYVYDKWSAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGVLDKG--FLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADGSAPSHGIIQSIFQCPKCNEAPLALKPKKNQQGWYIGCNNFPDCKNAVWLPTECKDASVLD----ECCPTCGDGYRM----------LKFRLSTPYYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVK 759 HSP 2 Score: 78.1814 bits (191), Expect = 1.694e-13 Identity = 63/228 (27.63%), Postives = 84/228 (36.84%), Query Frame = 3
Query: 1548 CGQIAATRKVSKEGPNKNKFFYTCSS-SKCQMFAWVES---------------------------------------GDVRNQNPHCSSTVMNSANTPQSSNNTRS-----DVNCNCGKPVNLLTVRKENQNKGRQFYSC----SQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPP----PAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
C Q A V K GPN + +Y C +C F W + G ++ S+ + T Q NT + +V CNCG+ + LTVRK+ N+GR FY+C C FF+W DE G +ST ++N PP P I PK+ R C LCR+ GHT KCP K
Sbjct: 1038 CQQPARQNTVRKNGPNLGRLYYKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQSNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLASQLTVRKDGPNQGRPFYACPTREKSCGFFKWGDEDQNQGASSTSWG-------------------SANRNPPGRSQPTAITSDGPKT-RRCGLCRKEGHTRNKCPRK 1245 HSP 3 Score: 65.855 bits (159), Expect = 1.144e-9 Identity = 28/38 (73.68%), Postives = 31/38 (81.58%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+D C AIKPNI+V RA FSEIT
Sbjct: 137 IWTDCDREGENIGYEIIDVCRAIKPNISVYRATFSEIT 174
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|O61660|TOP3_CAEEL (DNA topoisomerase 3 OS=Caenorhabditis elegans OX=6239 GN=top-3 PE=2 SV=1) HSP 1 Score: 226.868 bits (577), Expect = 8.398e-63 Identity = 117/270 (43.33%), Postives = 164/270 (60.74%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLK--QGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDE 926
FA +L G++P NG+KSD+AHPPIHPLK + L D+ +++EL+ RHFLAC+S+DA G ET V + + E F +GL + + YL VY Y+KW ++ +P Y GE F + I +T AP LTEADL+ LMDK+GIGTDATHAEHIEK+K R+Y+G G LIP LG+ LV+GY +G +S+PDLR+ E L+ IC+G +Q EVL+ + Y+ I+ + KI L + + +YLD+
Sbjct: 364 FANEVLQNGVNPRNGRKSDEAHPPIHPLKFTEKHQLQGDDWKVYELVVRHFLACVSQDAQGEETMVNLTVGT---------------------EKFHASGLRIRDMGYLKVYVYEKWGNRLLPTYTEGERFTDFELKIGDGKTQAPDFLTEADLISLMDKYGIGTDATHAEHIEKIKTREYIGVRPDGK--LIPSFLGLALVDGYDDMGFAMSKPDLRANLEIGLKEICDGRRQKQEVLDEQIGKYRAIFVESERKIGVLSQSLQRYLDK 610
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|Q9LVP1|TOP3A_ARATH (DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1) HSP 1 Score: 212.231 bits (539), Expect = 7.277e-57 Identity = 166/525 (31.62%), Postives = 244/525 (46.48%), Query Frame = 3
Query: 123 FATRLLS-EG---IHPHNGKKSDQAHPPIHPLKQGIG---LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACL-YCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPT---SSFRQELENLQDTSVKSTPVCH-CGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVES 1658
+A RLL EG +P NG D+AHPPIHP K G + D ++EL+ RH+LAC+S+ A+ ET V ID GE FS +G ++L +NYL+VY ++ W +PVY G+ F PT++++ + T P LL EADLL MDK GIGTDAT +HI+K+ R Y T P LG LV GY +G L +P+LR+ E D+ + G K EVL L K + + K +KL E + + + + DE++ + E VR C LC E + +RK++ + +GC NYP C++A+WLP L S T +C V KI K + GF D C+ CD + ++I S+ S + + T PSN Q + + I Q +S + P+ +S + +ST C+ CG R + E N+ + F++C + C FAW +S
Sbjct: 372 YAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVRHYLACVSQPAVAAETTVEIDIA---------------------GERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQFIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYA--TKDANTRFSPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLDARVKKSKLLEAMTIFFERSNNTDESE-------SQTAGEVVRRCNLCNESDMALRKNRDGNFMVGCMNYPQCRNAVWLPGPT----LEASVTTNVCQSCGPG-------PVYKILFKFRQIGIPPGF-----DVNHLGCVGGCDDILKQLIDICGTGSR--SQARRTPGTAPSNNIQGSNTRQS---NVCIHCQQRGHASTNCPSRVPASRNSRPTATNPRNDESTVSCNTCGSQCVLRTANTEA-NRGRQFFSCPTQGCSFFAWEDS 844 HSP 2 Score: 58.9214 bits (141), Expect = 1.364e-7 Identity = 25/46 (54.35%), Postives = 33/46 (71.74%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAV 139
LWL CDREGENI E+VD C A+K N+ ++RA FS + + H+AV
Sbjct: 120 LWLDCDREGENIAFEVVDVCRAVKHNLFIRRAHFSALIDRDIHEAV 165
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A5N3WYH4 (Uncharacterized protein OS=Muntiacus muntjak OX=9888 GN=FD754_011056 PE=4 SV=1) HSP 1 Score: 397.127 bits (1019), Expect = 1.312e-121 Identity = 245/665 (36.84%), Postives = 325/665 (48.87%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMN----SANTPQSS----NNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++ELI RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD+W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K L VL+ + YK+++ K KL E +++Y G + AP +L P + +R CP C ++++ K ++L CT +P C+ A+W PD+V L S++ C H + + S+ F+G I CD++ E L +RF P P++Q + S + + Q + P+ + + L S ++ C+CGQ A V KEGPN+ + FY CS C F W +SG P +S + S P S N +C CG+P TV+K+ N+GRQF++C+ QC FFQW DE G L P G + + S + K R C LC QPGHT CP +
Sbjct: 410 FARNILERGGPTPRNGNKSDQAHPPIHPTKYTDSLQGDEQRLYELIVRHFLACCSQDAQGQETTVEIDIAH---------------------ERFVAQGLMILARNYLDVYPYDRWSDKTLPVYEQGTCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKLVVLHQQVQKYKQVFIEAVAKARKLDEALSQYF-----GAAAEVAPQEAL-PAVPQPIRKCPQCGQDMVLKTRKSGGFYLSCTGFPECRSAVWFPDSV----LEASRDDSVCPVCQPHPVYRLKFKFKRGSLPPTMPLEFVGCIG-----------GCDETLREILALRFSRGAPQVG-------QPASQTSGHLQASQSLNRMDSGQQGQPRPPVTKPSKALTRTLPPPAIESEGNSVTCNCGQEALLLTVRKEGPNQGRQFYKCSGGGCSFFLWADSGHPGAGPPSSASRPLGRPPASGTLPGGSSRPGNGGSGGASCLCGQPAVTRTVQKDGPNQGRQFHTCAQPREQQCGFFQWVDENMAPGTFGGTL-------WPGSRGGTQGLEARSKRARAGSSDMGSAAKKPRKCSLCHQPGHTRPFCPQNR 1016
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A4X2KWD5 (DNA topoisomerase OS=Vombatus ursinus OX=29139 GN=TOP3A PE=3 SV=1) HSP 1 Score: 396.356 bits (1017), Expect = 1.568e-121 Identity = 250/681 (36.71%), Postives = 337/681 (49.49%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDE--APVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFH---------TSKPSSNFQ--NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVM-----NSANTPQSSN------NTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NGKKSDQAHPPIHP K L +E R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVY YDKW DK +P+Y G F+PT++ + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K VL + YK+++ NKL E +++Y E A VG E + P T++ CP C ++++K K ++L C YP C+ A+W PD+V L SK+ C H + L + S+ F+G I CD++ + L++R +P+++ Q SL+ + +NQ + + E + P++ S+ S+ E N T +C+CGQ A V KEGPN+ + FY C++ C F W +S + + NS N + ++ S C C +P TV+KE NKGRQF++CS QC FFQW+DE N + S + Q +KR S+ P K +R C LC QPGHT CP +
Sbjct: 375 FAQRILDRGGPTPRNGKKSDQAHPPIHPTKYTCNLQGNEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYIYDKWSDKVIPIYEKGSCFQPTTIEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKHVVLRQQIQKYKQVFIEAVAHANKLDEALSQYFGEMAAAVGQEE-------IYPAMPATIKKCPQCNKDMVLKKKKNGGFYLSCMGYPDCRKAIWFPDSV----LEASKDDSVCPVCKPHPVYRLKLKFKRGSVPPTMPLEFVGCIG-----------GCDETLMQILDLRSSNGPSRASQPAGRPTNHLQANQSLNRMGNNQGSYSISST------ERQTGPLNPSAGILVPSAATSESHN--------TVMCNCGQEALLLTVRKEGPNQGRHFYKCNTGSCNFFLWADSQQGTEMASFSRTRTLSHFQGNSTNFGGRQDGFGNHGSSSSSATCMCNQPAIKRTVQKEGPNKGRQFHTCSKPREQQCGFFQWADE-NVVPGTSGPVWFGGRGQGQGSEAKSKRPRTNSDTEP--------TTKKIRKCGLCHQPGHTRTTCPQNR 987 HSP 2 Score: 62.003 bits (149), Expect = 5.167e-6 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 128 IWTDCDREGENIGFEIIHVCKAVKPNLRVFRARFSEIT 165
BLAST of DNA topoisomerase vs. TrEMBL
Match: K9J3S6 (DNA topoisomerase (Fragment) OS=Desmodus rotundus OX=9430 PE=2 SV=1) HSP 1 Score: 392.119 bits (1006), Expect = 1.793e-121 Identity = 252/678 (37.17%), Postives = 326/678 (48.08%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFH---------TSKPSSNFQ-----NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTV--MNSANTPQ---SSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTS-NVWPPPAHINKGK-PKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD+W DK +PVY G F+P+++ + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K VL + YK+++ K KL E +++Y G+ + A ++ P E VR CP C ++++ K ++L CT +P C+ +W PD+V L S++ C H + + S+ FIG I CD++ E L +RF S+PS Q N + + + QP + T P K N P PT++ + SV VC+CGQ A V KEGPN+ + FY C C F W S P S+ S N P S + +C C + TV+K+ NKGRQF++C+ QC FFQW DE G P G K L S + P P+ + G K R C LC QPGHT CP +
Sbjct: 230 FAQSILERGGPTPRNGNKSDQAHPPIHPTKYTSSLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDRWSDKTLPVYERGSRFQPSTMQMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDAMGYEMSKPDLRAELEADLKLICEGKKDKCVVLRQQVQKYKQVFIEAVAKAKKLDEALSQYF-----GEGAEVAQQEAIYPAMLEPVRKCPQCNKDMVLKTKKSGGFYLSCTGFPECRSVMWFPDSV----LEASRDASVCPVCQPHPVYRLKFKFKRGSLPPTMPLEFIGCIG-----------GCDETLKEILGLRFSQGTPRAVPTASQPSGYLQAGRSSNRMDSSQHSHPQPPGTRQTRPLKALAPNLPP-------PTAAG-------ESNSV----VCNCGQEALLLTVRKEGPNQGRQFYKCQGGSCSFFLWANSSHPEAGGPPSSAIRHPTGSGNRPHGFGSPGDDSGGTSCLCSQATVTWTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENAAPGTLGA---------PPWPGGRGKTHGLESRSKRPRPSSSDPGSMAKKPRKCSLCHQPGHTRPFCPQNR 837
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A4W2HP29 (DNA topoisomerase OS=Bos indicus x Bos taurus OX=30522 GN=TOP3A PE=3 SV=1) HSP 1 Score: 393.66 bits (1010), Expect = 2.532e-121 Identity = 250/685 (36.50%), Postives = 331/685 (48.32%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF-----HTSKPSSNFQNSLSTIPS-------NQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRN--------------QNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKL--NMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L G P NG KSDQAHPPIHP K L DE R++ELI RHFLAC S+DA G ET V ID E F GL +L RNYLDVYPYD+W DK +PVY G F+P++V + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K L VL+ + YK+++ K KL E +++Y G + AP ++L P E +R CP C ++++ K ++LGCT +P C+ A+W PD+V L S++ C H + + S+ F+G I CD++ E L +RF S+P+S L S Q QP+ PP + P+ + + L S ++ C+CGQ A V KEGPN+ + FY C S C F W +SG ++P S T++ ++ + +C CG+P TV+KE N+GRQF++C+ QC FFQW DE G L +R Q R + K R C LC QPGHT CP +
Sbjct: 297 FARNILERGGPTPRNGNKSDQAHPPIHPTKYTDSLQGDEQRLYELIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYPYDRWSDKTLPVYEQGTCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKLVVLHQQVQKYKQVFIEAVAKARKLDEALSQYF-----GAATEVAPQDAL-PAVPEPIRKCPQCSRDMVLKTRKSGGFYLGCTGFPECRSAVWFPDSV----LEASRDVSVCPVCQPHPVYRLKFKFKRGSLPPTMPLEFVGCIG-----------GCDETLREILALRFSQGAARVSQPASQTSGYLQASQSLNRMDSGQQGQPR-----PPVTR--------------PSKALTRTLPPPAVESESNSVTCNCGQEALLLTVRKEGPNQGRQFYKCGSGGCSFFLWADSGHPGAGPPSSASRPPGGPLEHPPGSGTLLGGSSR--PGSGGSGGASCLCGQPAVTRTVQKEGPNQGRQFHTCAQPREHQCGFFQWVDENVAPGTFGGPLWPGSRRGTQGLEARSKRPRAGSSD---------TGSAAKKPRKCSLCHQPGHTRPFCPQNR 907
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A4X2KWG1 (DNA topoisomerase OS=Vombatus ursinus OX=29139 GN=TOP3A PE=3 SV=1) HSP 1 Score: 395.586 bits (1015), Expect = 2.672e-121 Identity = 250/681 (36.71%), Postives = 337/681 (49.49%), Query Frame = 3
Query: 123 FATRLLSEG-IHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDE--APVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFH---------TSKPSSNFQ--NSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVM-----NSANTPQSSN------NTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA R+L G P NGKKSDQAHPPIHP K L +E R++E I RHFLAC S+DA G ET V ID E F GL +L RNYLDVY YDKW DK +P+Y G F+PT++ + ETS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T P FL PG LGMGLVEGY +G +S+PDLR++ EADL+LIC G K VL + YK+++ NKL E +++Y E A VG E + P T++ CP C ++++K K ++L C YP C+ A+W PD+V L SK+ C H + L + S+ F+G I CD++ + L++R +P+++ Q SL+ + +NQ + + E + P++ S+ S+ E N T +C+CGQ A V KEGPN+ + FY C++ C F W +S + + NS N + ++ S C C +P TV+KE NKGRQF++CS QC FFQW+DE N + S + Q +KR S+ P K +R C LC QPGHT CP +
Sbjct: 370 FAQRILDRGGPTPRNGKKSDQAHPPIHPTKYTCNLQGNEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQ---------------------ERFVAHGLMILARNYLDVYIYDKWSDKVIPIYEKGSCFQPTTIEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLT-PDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKHVVLRQQIQKYKQVFIEAVAHANKLDEALSQYFGEMAAAVGQEE-------IYPAMPATIKKCPQCNKDMVLKKKKNGGFYLSCMGYPDCRKAIWFPDSV----LEASKDDSVCPVCKPHPVYRLKLKFKRGSVPPTMPLEFVGCIG-----------GCDETLMQILDLRSSNGPSRASQPAGRPTNHLQANQSLNRMGNNQGSYSISST------ERQTGPLNPSAGILVPSAATSESHN--------TVMCNCGQEALLLTVRKEGPNQGRHFYKCNTGSCNFFLWADSQQGTEMASFSRTRTLSHFQGNSTNFGGRQDGFGNHGSSSSSATCMCNQPAIKRTVQKEGPNKGRQFHTCSKPREQQCGFFQWADE-NVVPGTSGPVWFGGRGQGQGSEAKSKRPRTNSDTEP--------TTKKIRKCGLCHQPGHTRTTCPQNR 982 HSP 2 Score: 62.003 bits (149), Expect = 5.158e-6 Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG EI+ C A+KPN+ V RA+FSEIT
Sbjct: 148 IWTDCDREGENIGFEIIHVCKAVKPNLRVFRARFSEIT 185
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 380.178 bits (975), Expect = 1.115e-118 Identity = 244/689 (35.41%), Postives = 341/689 (49.49%), Query Frame = 3
Query: 123 FATRLL-SEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVW-FLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF---HTSKPSS--NFQNSLSTIPSNQAQP---KVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVES-----GDVRNQNP-----HCSSTVMNSANTPQ-------SSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDN------KRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L S G P NG KSDQAHPPIHP K L +E R++E I RHFLAC SKDA G E V ID E FS +GL+++ RNYLDVYPY+KW +K +P+Y F+P+++ + + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG+LGMGLVEGY+ +G +S+PDLR++ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL G + VR CP C +++RK K + FL C YPACK A+W PDTV V S++ TC H H + S+ + F+G I CD++ E LN+++ + +P++ N +L + N+ P + S PP +++P+ S D Q +V VC+CG A V KEGPN+ + FY C++ C+ F W + G ++ P S + N PQ ++ +T + CNC +P TV K+ NKGR F++C QC FFQW+DE P + +S GDN K+ ++S+ P K R+C +C QPGHT CP +
Sbjct: 391 FAQGILESGGPTPRNGNKSDQAHPPIHPTKYTNSLQGNEKRLYEFIVRHFLACCSKDAQGQEVIVDIDIAE---------------------EKFSASGLTIIARNYLDVYPYEKWFNKVIPLYTANTTFQPSAIEMVEGQTSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLTAD--QRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKDKASVLRHHVAKYKAVFIESAKKAKKLDEALSNYL-----GQAQEYTEQEEQELEMPLPVRKCPQCNRDMVLRKRKDSTGKFLSCLGYPACKAAVWFPDTVLEV----SRDDSVCPTCRPHPVHMLKFKFKRGSLPPGMDLEFVGCIG-----------GCDETLREVLNLKYLRGASRQPNAPRNNNTALQSRSENRPPPPRLSAVSSRPPLTNPAQSRPMQLPSSD-------------QSNAV----VCNCGVDALLLTVRKEGPNQGRQFYKCNTGNCKFFLWADESAEQHGSAGSRGPAGQSFQPSRPSVGFGNGPQERHGSRGAAGDTGGEAMCNCNEPAVQRTVMKDGPNKGRAFHTCGKPRDQQCGFFQWADENVPPA----------AVRSTTGGGDNSTWNGSKKTGVSSSKAP--------AAKRPRTCGICHQPGHTRTTCPQGR 1001 HSP 2 Score: 60.077 bits (144), Expect = 7.987e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG E+++ C A+K NI V RA+FSEIT
Sbjct: 143 IWTDCDREGENIGFEVINVCKAVKANIQVFRARFSEIT 180
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 374.015 bits (959), Expect = 4.258e-117 Identity = 244/704 (34.66%), Postives = 341/704 (48.44%), Query Frame = 3
Query: 123 FATRLL-SEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVW-FLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF------------------HTSKPSS--NFQNSLSTIPSNQAQP---KVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVES-----GDVRNQNP-----HCSSTVMNSANTPQ-------SSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDN------KRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA +L S G P NG KSDQAHPPIHP K L +E R++E I RHFLAC SKDA G E V ID E FS +GL+++ RNYLDVYPY+KW +K +P+Y F+P+++ + + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T +PG+LGMGLVEGY+ +G +S+PDLR++ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL G + VR CP C +++RK K + FL C YPACK A+W PDTV V S++ TC H H + S+ + F+G I CD++ E LN+++ + +P++ N +L + N+ P + S PP +++P+ S D Q +V VC+CG A V KEGPN+ + FY C++ C+ F W + G ++ P S + N PQ ++ +T + CNC +P TV K+ NKGR F++C QC FFQW+DE P + +S GDN K+ ++S+ P K R+C +C QPGHT CP +
Sbjct: 308 FAQGILESGGPTPRNGNKSDQAHPPIHPTKYTNSLQGNEKRLYEFIVRHFLACCSKDAQGQEVIVDIDIAE---------------------EKFSASGLTIIARNYLDVYPYEKWFNKVIPLYTANTTFQPSAIEMVEGQTSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLTAD--QRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKDKASVLRHHVAKYKAVFIESAKKAKKLDEALSNYL-----GQAQEYTEQEEQELEMPLPVRKCPQCNRDMVLRKRKDSTGKFLSCLGYPACKAAVWFPDTVLEV----SRDDSVCPTCRPHPVHMLKFKFKRGSLPPGMDLEFVGCIG-----------GCDETLREVLNLKYLRGGGSGGDSGIQVIQRTASRQPNAPRNNNTALQSRSENRPPPPRLSAVSSRPPLTNPAQSRPMQLPSSD-------------QSNAV----VCNCGVDALLLTVRKEGPNQGRQFYKCNTGNCKFFLWADESAEQHGSAGSRGPAGQSFQPSRPSVGFGNGPQERHGSRGAAGDTGGEAMCNCNEPAVQRTVMKDGPNKGRAFHTCGKPRDQQCGFFQWADENVPPA----------AVRSTTGGGDNSTWNGSKKTGVSSSKAP--------AAKRPRTCGICHQPGHTRTTCPQGR 933 HSP 2 Score: 60.077 bits (144), Expect = 7.677e-9 Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEIT 115
+W CDREGENIG E+++ C A+K NI V RA+FSEIT
Sbjct: 60 IWTDCDREGENIGFEVINVCKAVKANIQVFRARFSEIT 97
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3b (DNA topoisomerase III beta [Source:NCBI gene;Acc:103043516]) HSP 1 Score: 118.242 bits (295), Expect = 4.583e-27 Identity = 83/248 (33.47%), Postives = 125/248 (50.40%), Query Frame = 3
Query: 135 LLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIY 866
LL+EGI+ P G + HPPI P++ L + R++E ITRHF+A +S D +T + + R+ +E GFS TG +++ + +V P+ +++ +P G+ F V + + +TS P LTEA+L+ LM+KHGIGTDA+ HI + R+YV T L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K +
Sbjct: 370 LLAEGINRPRKGADAGD-HPPITPMRSATESELGSESWRLYEYITRHFIATVSPDCKYLQTTI------------------SFRIGTE---GFSCTGKTLISAGFTEVMPWQGIPLEEALPTCEKGDAFTVDEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYV--TVENGRKLKPTNLGIVLVHGYYKIDVELVLPTIRSAVEKQLNLIALGKANFHQVLQHTLDIFKRKF 593
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 375.555 bits (963), Expect = 7.646e-118 Identity = 239/664 (35.99%), Postives = 339/664 (51.05%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFH--TSKPSSNFQNSLSTIPSNQA--QPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQW-SDEPNPIGNASTKLNMKRSFQS---PHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FA RLL G +P NG KSDQAHPPIHP K LN ++ R++E I RHFLAC S+DA G ET V I+ GE F GL + RNYLDVYPYDKW K +PVY G F+P+++ ++ ETS P LL+EADL+ LM+KHGIGTDATHA+HIE +K R YVG T P FL PG+LGMGLVEGY +G +S+PDLR++ EADL+LIC G K VL + YK+++ +K KL E ++ YL E N++ L P VR CP C+ ++++++K + L C +P C+ A++ PD V L +++ C + H K S+ T ++G + CD + ++++ + P+ + QN S++ + QP S ++ + P + P R E +S VC+CG A V KEG NK + FY CSSS C F W + D ++ + + +S+ + ++ +V C+C P + T+ K+ NKGRQFY+CS QC FF W +DEP+ +S+ + + S P DN R TSN H ++ R+C +C QPGH CP K
Sbjct: 392 FAQRLLEAGPNPRNGTKSDQAHPPIHPTKYTADLNGNDQRLYEFIVRHFLACCSQDAQGQETTVEIEIA---------------------GERFFANGLMITARNYLDVYPYDKWNAKVIPVYTTGVKFQPSNIEMTDGETSPPQLLSEADLISLMEKHGIGTDATHADHIETIKSRMYVGLT-PAQRFL-PGELGMGLVEGYDAMGYEMSKPDLRAELEADLKLICEGKKNNTTVLQQQIQKYKQVFLEAVSKAEKLDEALSCYLGEPQQSIANEETIPRRLEP-----VRKCPQCKSNMVLKQTKDGKFMLTCLGFPNCRAAVFFPDFV----LEATRDESICQFCQPNAIHRLKFKFKKNSVIPCTPLDYVGCVG-----------GCDDVINDIADLKYLRPATTPNQSRQNQPSSVARQRGTCQP----SNAYVQRPPQQAPPPMAMLQIPPG--RNE---------ESAVVCNCGIEATLLTVKKEGINKGRMFYKCSSSVCNFFLWADQQDGGFRSAQSNQSSFHSSAVSRGGHD---EVMCSCNNPAVMRTIVKDGPNKGRQFYTCSKPRDEQCGFFLWMADEPSNSDLSSSNCDFAYAAHSVTRPSPRPDNARP--TSNNRTAGEHTTV---RAKRTCGICHQPGHIRTTCPMNK 989 HSP 2 Score: 67.781 bits (164), Expect = 6.709e-12 Identity = 29/45 (64.44%), Postives = 34/45 (75.56%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKA 136
+W CDREGENIG EI+D C A+KPN+ V RA+FSEIT Q H A
Sbjct: 145 IWTDCDREGENIGFEIIDVCKAVKPNLQVFRARFSEITQQSIHNA 189
BLAST of DNA topoisomerase vs. Ensembl Yeast
Match: TOP3 (DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination [Source:SGD;Acc:S000004224]) HSP 1 Score: 160.614 bits (405), Expect = 4.393e-42 Identity = 91/254 (35.83%), Postives = 138/254 (54.33%), Query Frame = 3
Query: 156 PHNGKKSDQAHPPIHP---LKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGM-NFLIPGKLGMGLVEGYSLVG--QLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKL 896
P +G D+AHPPIHP L ++ E R++E + RHFLAC S+DA G +++D W V E FS +GL VLERN+LDVYP+ +W K++P + + + + T+ P +TE++L++LMD +GIGTDAT AEHI+K++ R+YV + G +L P LG+ LV G+ +G ++P R + E DL+ IC G +V+ + Y++ + TN N L
Sbjct: 413 PRSGSHDDKAHPPIHPIVSLGPEANVSPVERRVYEYVARHFLACCSEDAKGQSMTLVLD----------WAV-----------ERFSASGLVVLERNFLDVYPWARWETTKQLPRLEMNALVDIAKAEMKAGTTAPPKPMTESELILLMDTNGIGTDATIAEHIDKIQVRNYVRSEKVGKETYLQPTTLGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVVKDIVEKYRKYWHKTNACKNTL 645
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Match: EDO40018 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S883]) HSP 1 Score: 338.191 bits (866), Expect = 6.820e-104 Identity = 215/615 (34.96%), Postives = 309/615 (50.24%), Query Frame = 3
Query: 90 SALNFLKLLFRFATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEE-TVRGCPLCEG-MILIRKSKK------NVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSK--CQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVN--------CNCGKPVNLLTVRKENQNKGRQFYSCSQ-----CSFFQWSDE 1865
S +++++ + FA+ +L+ G +P G K+D AHPPIHP + L ++ R++E I RHFLAC S+DA G ET V ID GE F+ TGL + RNYLDVYPYD+W K +PVY E F+PTS+ + ET+ P LLTEADL+ LM+KHGIGTDATHA+HIE +K R YVG G G +PG+LGMGLVEGY L+ S+P LRS EADL+ IC+GTK VL + YKE + + KL + ++ Y GD D L+PL + VR CP CE + +RK+K+ + + +GC +P C+ +++LP +V LA + + C H + S+ T ++G I CD+ E L+I+ + +N+ + PS + Q + + F + T+ Q S ++ VC CG A V K+GPN+ + FY CS + C F W ++ P T+ +S N S N++ ++ CNC +P TV K+ NKGR FYSCS+ C FF+W+D+
Sbjct: 342 SMISYIEFVTGFASDVLANGPNPRQGSKTDNAHPPIHPTQYTNNLQGNDKRLYEFIVRHFLACCSQDAQGRETSVEIDIA---------------------GEKFTATGLMITARNYLDVYPYDRWSTKVIPVYETNEEFQPTSIEMVDGETTPPPLLTEADLIALMEKHGIGTDATHADHIETIKSRSYVGVQGDGT--FLPGQLGMGLVEGYDLMHFEFSKPKLRSGLEADLKRICDGTKSGAVVLREQIAKYKENFIQAAQEAIKLDQALSNYF-----GDPADFIE-QELDPLAADPIVRPCPRCEQHQMTLRKTKEGRQVKLSFYMIGCQGFPQCRASIFLPKSV----LALTVDESVCPVCRPRDVHMLRFTFKRGSVPPMTPLQYVGCIG-----------GCDEMLTETLDIKDIS-------RNTRGSQPS-RGQSRGRSQSSQFGASQTSASGRGRGRGTRTTGSNQSSRYYVCYSDENAIVCTCGNAAIQLTVRKDGPNQGRQFYKCSQREGGCDFFLWADANGPLPSTPR--PTLWSSTNGSSSWNDSGKRLDHVIGDELVCNCRQPAVSRTVNKDGPNKGRPFYSCSKPIGQSCGFFKWADQ 902 HSP 2 Score: 60.4622 bits (145), Expect = 2.250e-9 Identity = 26/45 (57.78%), Postives = 31/45 (68.89%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKA 136
+W CDREGENIG EI+D C + P I V RA+FSEIT Q +A
Sbjct: 112 IWTDCDREGENIGHEIIDVCTKVCPRIKVYRARFSEITPQSVQRA 156 HSP 3 Score: 52.373 bits (124), Expect = 5.754e-7 Identity = 23/47 (48.94%), Postives = 28/47 (59.57%), Query Frame = 3
Query: 1749 VNCNCGKPVNLLTVRKENQNKGRQFYSCSQ----CSFFQWSDEPNPI 1877
+ C CG LTVRK+ N+GRQFY CSQ C FF W+D P+
Sbjct: 780 IVCTCGNAAIQLTVRKDGPNQGRQFYKCSQREGGCDFFLWADANGPL 826
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Match: EDO44262 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:A7RW77]) HSP 1 Score: 110.538 bits (275), Expect = 4.963e-25 Identity = 78/243 (32.10%), Postives = 117/243 (48.15%), Query Frame = 3
Query: 186 HPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIG-EGFSLTGLSVLERNYLDVYPYDKWVDKE-VPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKE 902
HPPI P++ L D R+++ ITRHFLA LS D +T V + +IG E FS TG +V + + V + D E +P + G+I + V + + +T P LTE++L+ LM+KHGIGTDA+ + HI + R+YV G L P LG+ LV GY + L P +RS E L LI +G VL+H + + + + ++ + E
Sbjct: 385 HPPITPMRCASESELGGDPWRLYDFITRHFLATLSPDCKYLQTTV----------------------KFKIGKEAFSFTGKAVTDPGFTSVMTWQAISDDERMPPFNKGDICQVAEVKLDERQTLPPDFLTESELITLMEKHGIGTDASISVHINNICERNYV-QIQSGRK-LAPTSLGIVLVHGYQKIDPELVLPTMRSAVEEQLNLIAHGKADFNAVLHHAIGIFTQKFTYFVKTVSSMDE 603
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 360.147 bits (923), Expect = 6.375e-112 Identity = 248/688 (36.05%), Postives = 330/688 (47.97%), Query Frame = 3
Query: 123 FATRLLSE--GIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLN-PLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF--------HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVE---------------SGDVRNQNPHCSSTVMNSANTPQSSNNTRS----DVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE--PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
FA R+L + G +P GKKSDQAHPPIHP K L +EAR++E I RHFLAC+S+DALG ET V ID E FS +GL ++ RNYLDVYPYDKW K +PVY F+P+++ + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T FL PG+LGMGLVEGY+ +G +S+P+LRS+ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL A E +A L PL VR CP C M+L RK + + FL C YPACK A+W PD V V S++ TC H + S+ F+G I CD++ E L++++ + +S P A P+ ++ PP + + P P + N D VC+CGQ A V K+GPN+ + FY C+S C F W + S Q P S+ N++ + S CNC + TV+K+ NKGR F++C QC FFQW+DE P P G + D +R + + PP A K R C C PGHT CP +
Sbjct: 307 FAQRVLDQPGGPNPRQGKKSDQAHPPIHPTKYTSSLQGNEARVYEFIVRHFLACVSQDALGQETVVDIDIAQ---------------------EKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQPSAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTA-DQRFL-PGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAA---LEIPEAQQQDLEMPL---PVRKCPNCGRDMVLKRKREGSSKFLTCVGYPACKTAVWFPDMVLDV----SRDESICPTCQPSPVHMLKFKFRRGSLPPMMPLEFVGCIG-----------GCDETLREVLDLKYLRGAGAGGAGGGGRGDDPHSPPPRPPRAAPPRTQRAIPP----VPSVPAWNPEPRPPPGGSSVAINNSNDVI-----VCNCGQEAVLLTVRKDGPNQGRQFYKCNSGSCNFFLWADQPSQQEAPQSRGPTPSAQRIQQLPRPSAGFRNASGGRRGHEAGGSGHSGQTLCNCNQTAVTRTVQKDGPNKGRMFHTCGKPREQQCGFFQWADENVPPPDGGF-------------NRLEDGRRKVMGDSNKPPTA-------KKPRHCGNCHMPGHTRVTCPQR 921 HSP 2 Score: 66.2402 bits (160), Expect = 8.709e-11 Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAVVRRNTST 160
+W CDREGENIG E++D C A+KPN+ V RAKFSEIT A +RR T
Sbjct: 60 IWTDCDREGENIGFEVIDVCKAVKPNLQVFRAKFSEIT-----PASIRRACET 107
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 362.459 bits (929), Expect = 7.826e-112 Identity = 250/687 (36.39%), Postives = 334/687 (48.62%), Query Frame = 3
Query: 123 FATRLLSE--GIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLN-PLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF--------HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVE---------------SGDVRNQNPHCSSTVMNSANTPQSSNNTRS----DVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE-PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
FA R+L + G +P GKKSDQAHPPIHP K L +EAR++E I RHFLAC+S+DALG ET V ID E FS +GL ++ RNYLDVYPYDKW K +PVY F+P+++ + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T FL PG+LGMGLVEGY+ +G +S+P+LRS+ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL A E +A L PL VR CP C M+L RK + + FL C YPACK A+W PD V V S++ TC H + S+ F+G I CD++ E L++++ + +S P A P+ ++ PP + + P P + N D VC+CGQ A V K+GPN+ + FY C+S C F W + S Q P S+ N++ + S CNC + TV+K+ NKGR F++C QC FFQW+DE P G +S+ R S H + +R + + PP A K R C C PGHT CP +
Sbjct: 408 FAQRVLDQPGGPNPRQGKKSDQAHPPIHPTKYTSSLQGNEARVYEFIVRHFLACVSQDALGQETVVDIDIAQ---------------------EKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQPSAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTA-DQRFL-PGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAA---LEIPEAQQQDLEMPL---PVRKCPNCGRDMVLKRKREGSSKFLTCVGYPACKTAVWFPDMVLDV----SRDESICPTCQPSPVHMLKFKFRRGSLPPMMPLEFVGCIG-----------GCDETLREVLDLKYLRGAGAGGAGGGGRGDDPHSPPPRPPRAAPPRTQRAIPP----VPSVPAWNPEPRPPPGGSSVAINNSNDVI-----VCNCGQEAVLLTVRKDGPNQGRQFYKCNSGSCNFFLWADQPSQQEAPQSRGPTPSAQRIQQLPRPSAGFRNASGGRRGHEAGGSGHSGQTLCNCNQTAVTRTVQKDGPNKGRMFHTCGKPREQQCGFFQWADENVPPPGKSSSSKRSTRVGSSSHPNSNGRRKVMGDSNKPPTA-------KKPRHCGNCHMPGHTRVTCPQR 1034 HSP 2 Score: 66.2402 bits (160), Expect = 8.860e-11 Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAVVRRNTST 160
+W CDREGENIG E++D C A+KPN+ V RAKFSEIT A +RR T
Sbjct: 161 IWTDCDREGENIGFEVIDVCKAVKPNLQVFRAKFSEIT-----PASIRRACET 208
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 360.533 bits (924), Expect = 1.830e-111 Identity = 248/688 (36.05%), Postives = 330/688 (47.97%), Query Frame = 3
Query: 123 FATRLLSE--GIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLN-PLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF--------HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVE---------------SGDVRNQNPHCSSTVMNSANTPQSSNNTRS----DVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE--PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
FA R+L + G +P GKKSDQAHPPIHP K L +EAR++E I RHFLAC+S+DALG ET V ID E FS +GL ++ RNYLDVYPYDKW K +PVY F+P+++ + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T FL PG+LGMGLVEGY+ +G +S+P+LRS+ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL A E +A L PL VR CP C M+L RK + + FL C YPACK A+W PD V V S++ TC H + S+ F+G I CD++ E L++++ + +S P A P+ ++ PP + + P P + N D VC+CGQ A V K+GPN+ + FY C+S C F W + S Q P S+ N++ + S CNC + TV+K+ NKGR F++C QC FFQW+DE P P G + D +R + + PP A K R C C PGHT CP +
Sbjct: 367 FAQRVLDQPGGPNPRQGKKSDQAHPPIHPTKYTSSLQGNEARVYEFIVRHFLACVSQDALGQETVVDIDIAQ---------------------EKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQPSAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTA-DQRFL-PGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAA---LEIPEAQQQDLEMPL---PVRKCPNCGRDMVLKRKREGSSKFLTCVGYPACKTAVWFPDMVLDV----SRDESICPTCQPSPVHMLKFKFRRGSLPPMMPLEFVGCIG-----------GCDETLREVLDLKYLRGAGAGGAGGGGRGDDPHSPPPRPPRAAPPRTQRAIPP----VPSVPAWNPEPRPPPGGSSVAINNSNDVI-----VCNCGQEAVLLTVRKDGPNQGRQFYKCNSGSCNFFLWADQPSQQEAPQSRGPTPSAQRIQQLPRPSAGFRNASGGRRGHEAGGSGHSGQTLCNCNQTAVTRTVQKDGPNKGRMFHTCGKPREQQCGFFQWADENVPPPDGGF-------------NRLEDGRRKVMGDSNKPPTA-------KKPRHCGNCHMPGHTRVTCPQR 981 HSP 2 Score: 66.2402 bits (160), Expect = 9.473e-11 Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAVVRRNTST 160
+W CDREGENIG E++D C A+KPN+ V RAKFSEIT A +RR T
Sbjct: 120 IWTDCDREGENIGFEVIDVCKAVKPNLQVFRAKFSEIT-----PASIRRACET 167
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 360.533 bits (924), Expect = 3.758e-111 Identity = 248/688 (36.05%), Postives = 330/688 (47.97%), Query Frame = 3
Query: 123 FATRLLSE--GIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLN-PLTEETVRGCPLC-EGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRF--------HTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVE---------------SGDVRNQNPHCSSTVMNSANTPQSSNNTRS----DVNCNCGKPVNLLTVRKENQNKGRQFYSCS-----QCSFFQWSDE--PNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSK 2072
FA R+L + G +P GKKSDQAHPPIHP K L +EAR++E I RHFLAC+S+DALG ET V ID E FS +GL ++ RNYLDVYPYDKW K +PVY F+P+++ + +TS P LLTEADL+ LM+KHGIGTDATHAEHIE +K R YVG T FL PG+LGMGLVEGY+ +G +S+P+LRS+ EADL+L+ G K VL H++ YK ++ + K KL E ++ YL A E +A L PL VR CP C M+L RK + + FL C YPACK A+W PD V V S++ TC H + S+ F+G I CD++ E L++++ + +S P A P+ ++ PP + + P P + N D VC+CGQ A V K+GPN+ + FY C+S C F W + S Q P S+ N++ + S CNC + TV+K+ NKGR F++C QC FFQW+DE P P G + D +R + + PP A K R C C PGHT CP +
Sbjct: 410 FAQRVLDQPGGPNPRQGKKSDQAHPPIHPTKYTSSLQGNEARVYEFIVRHFLACVSQDALGQETVVDIDIAQ---------------------EKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQPSAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTA-DQRFL-PGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAA---LEIPEAQQQDLEMPL---PVRKCPNCGRDMVLKRKREGSSKFLTCVGYPACKTAVWFPDMVLDV----SRDESICPTCQPSPVHMLKFKFRRGSLPPMMPLEFVGCIG-----------GCDETLREVLDLKYLRGAGAGGAGGGGRGDDPHSPPPRPPRAAPPRTQRAIPP----VPSVPAWNPEPRPPPGGSSVAINNSNDVI-----VCNCGQEAVLLTVRKDGPNQGRQFYKCNSGSCNFFLWADQPSQQEAPQSRGPTPSAQRIQQLPRPSAGFRNASGGRRGHEAGGSGHSGQTLCNCNQTAVTRTVQKDGPNKGRMFHTCGKPREQQCGFFQWADENVPPPDGGF-------------NRLEDGRRKVMGDSNKPPTA-------KKPRHCGNCHMPGHTRVTCPQR 1024 HSP 2 Score: 66.2402 bits (160), Expect = 9.216e-11 Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAVVRRNTST 160
+W CDREGENIG E++D C A+KPN+ V RAKFSEIT A +RR T
Sbjct: 163 IWTDCDREGENIGFEVIDVCKAVKPNLQVFRAKFSEIT-----PASIRRACET 210
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3b (DNA topoisomerase III beta [Source:NCBI gene;Acc:101167966]) HSP 1 Score: 111.694 bits (278), Expect = 6.340e-25 Identity = 124/472 (26.27%), Postives = 194/472 (41.10%), Query Frame = 3
Query: 135 LLSEGIH-PHNGKKSDQAHPPIHPLKQGIG--LNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIG-EGFSLTGLSVLERNYLDVYPYDKW-VDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVG-NTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGV-----------------------AKYLDEAPV------GDENDDAPWNSLNPLTEET---------------VRG-----CPLCEGMILIRKSKKNVWFLGCTNYPACKH---ALWLPDTVEH----VMLAKSKETEAIITCSNHH------EHDHNLAVNKISIKI-QTNQTFIGF-----IFFDEDRRFTACLYCDKSFCEALNIR 1328
LLS G++ P G HPPI P++ L D R++E ITRHF+A +S+D ++ + IG E FS G + + Y + P+ +++ +P G+ F + + + +T P LTEA+L+ LM+KHGIGTDA+ HI V R+YV TG L P LG+ LV GY + L P +RS E L LI G +VL H L+ +K + I+ + E + KY+ P DE + P N L +E RG CP C R KK + CT +P+C+H AL + VE ++L + + + C+ + EH H + V++ S ++ + + F + D + T C++CD F +A+ ++
Sbjct: 370 LLSAGLNRPRKGNDVGD-HPPITPMRAASEGELGSDGWRLYEYITRHFIATVSQDCRYLQSTIEFS----------------------IGTERFSCGGKTPISPGYTALMPWQGVPLEEALPTCQPGDAFTVDELRLVERQTGPPDYLTEAELITLMEKHGIGTDASIPVHINNVCQRNYVTVETG---RRLKPTNLGVVLVHGYYKIDAELVLPTIRSAVEKQLSLIALGKANFQQVLQHTLDIFKRKFHYFVDSISSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYNVPQNGAIKLYKELRCPLDDFELVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECT-HPSCQHSLSALGIGQCVECESGVLVLDPTSGPKWRMACNRCNVLVHLFEHAHKVQVSQDSCEVCDASLVMVDFNKARTPLPEGDTQHTGCVFCDPLFQDAVELK 814
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081337.1 (SMESG000081337.1) HSP 1 Score: 1367.06 bits (3537), Expect = 0.000e+0 Identity = 651/651 (100.00%), Postives = 651/651 (100.00%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 2075
FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK
Sbjct: 360 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYLDEAPVGDENDDAPWNSLNPLTEETVRGCPLCEGMILIRKSKKNVWFLGCTNYPACKHALWLPDTVEHVMLAKSKETEAIITCSNHHEHDHNLAVNKISIKIQTNQTFIGFIFFDEDRRFTACLYCDKSFCEALNIRFHTSKPSSNFQNSLSTIPSNQAQPKVMKSTPPFKKEIKNQPISQSSFDFPTSSFRQELENLQDTSVKSTPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQPGHTSRKCPSKK 1010 HSP 2 Score: 84.3445 bits (207), Expect = 1.880e-16 Identity = 38/46 (82.61%), Postives = 41/46 (89.13%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAV 139
+W CDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQ +A+
Sbjct: 112 IWTDCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQSVTRAL 157
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081321.1 (SMESG000081321.1) HSP 1 Score: 298.516 bits (763), Expect = 3.453e-96 Identity = 141/147 (95.92%), Postives = 144/147 (97.96%), Query Frame = 3
Query: 1599 NKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIALTSNVWPPPAHINKGKPKSVRSCLLCRQ 2039
+KFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRI LTSNVW PPAHINKGKPKSVRSCLL ++
Sbjct: 128 DKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQCSFFQWSDEPNPIGNASTKLNMKRSFQSPHEFGDNKRIDLTSNVWSPPAHINKGKPKSVRSCLLSKK 274
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081353.1 (SMESG000081353.1) HSP 1 Score: 295.819 bits (756), Expect = 7.873e-90 Identity = 149/266 (56.02%), Postives = 189/266 (71.05%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYL 920
FA +L +GI P G K+D AHPPI PLK GIGL DE +FELITRHFLACLSKDA T++LIDCISK + D+W + + S +GE FSLTG V E+NYLDVYPY+K +D +P Y IG+IFEP+ V I +T PS LT+ DL+ LMDK+GIGTDATHAEHI K+K R+Y P NF +P KLG+GL++ Y+ +G +++P LRS+FEADL+ ICNGTK P EVL HYL+ YK I+ + I+KL++ V KYL
Sbjct: 310 FAKNILLDGITPMCGNKNDNAHPPIIPLKLGIGLAFDENHLFELITRHFLACLSKDAQCSRTEILIDCISKDHGRDTWKHKDSKIACSGLGEEFSLTGTIVREKNYLDVYPYEKLLDTFIPNYEIGDIFEPSCVGIKMGKTCPPSFLTDVDLISLMDKYGIGTDATHAEHIYKIKVREYTCLKYP--NFTVPTKLGIGLIDAYNYLGINIAQPMLRSKFEADLKEICNGTKDPNEVLRHYLDEYKSIFLESIKNIDKLQDIVLKYL 573 HSP 2 Score: 64.3142 bits (155), Expect = 2.114e-10 Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAV 139
+W CDREGENIGMEIVD CLA+ I VKRA+FSEIT + +A+
Sbjct: 62 IWTDCDREGENIGMEIVDVCLAVNSKIDVKRARFSEITAESVTRAI 107 HSP 3 Score: 53.5286 bits (127), Expect = 4.556e-7 Identity = 34/112 (30.36%), Postives = 46/112 (41.07%), Query Frame = 3
Query: 1530 STPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQ---CSFFQW 1856
+ P C CG K + P ++ Y C + KC + + + + N T+ C CGKP L T K N NKGRQ+YSC+Q C FF W
Sbjct: 612 NVPNCFCG------KPTSSTPKLDQLLYNCKNKKCDFYLLI------------ENEKEKKKSEIDDDNVTK----CYCGKPAKLKTSLKNNANKGRQYYSCNQITNCKFFCW 701
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081353.1 (SMESG000081353.1) HSP 1 Score: 295.819 bits (756), Expect = 7.932e-90 Identity = 149/266 (56.02%), Postives = 189/266 (71.05%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYL 920
FA +L +GI P G K+D AHPPI PLK GIGL DE +FELITRHFLACLSKDA T++LIDCISK + D+W + + S +GE FSLTG V E+NYLDVYPY+K +D +P Y IG+IFEP+ V I +T PS LT+ DL+ LMDK+GIGTDATHAEHI K+K R+Y P NF +P KLG+GL++ Y+ +G +++P LRS+FEADL+ ICNGTK P EVL HYL+ YK I+ + I+KL++ V KYL
Sbjct: 308 FAKNILLDGITPMCGNKNDNAHPPIIPLKLGIGLAFDENHLFELITRHFLACLSKDAQCSRTEILIDCISKDHGRDTWKHKDSKIACSGLGEEFSLTGTIVREKNYLDVYPYEKLLDTFIPNYEIGDIFEPSCVGIKMGKTCPPSFLTDVDLISLMDKYGIGTDATHAEHIYKIKVREYTCLKYP--NFTVPTKLGIGLIDAYNYLGINIAQPMLRSKFEADLKEICNGTKDPNEVLRHYLDEYKSIFLESIKNIDKLQDIVLKYL 571 HSP 2 Score: 64.3142 bits (155), Expect = 2.166e-10 Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAV 139
+W CDREGENIGMEIVD CLA+ I VKRA+FSEIT + +A+
Sbjct: 60 IWTDCDREGENIGMEIVDVCLAVNSKIDVKRARFSEITAESVTRAI 105 HSP 3 Score: 53.5286 bits (127), Expect = 5.131e-7 Identity = 34/112 (30.36%), Postives = 46/112 (41.07%), Query Frame = 3
Query: 1530 STPVCHCGQIAATRKVSKEGPNKNKFFYTCSSSKCQMFAWVESGDVRNQNPHCSSTVMNSANTPQSSNNTRSDVNCNCGKPVNLLTVRKENQNKGRQFYSCSQ---CSFFQW 1856
+ P C CG K + P ++ Y C + KC + + + + N T+ C CGKP L T K N NKGRQ+YSC+Q C FF W
Sbjct: 610 NVPNCFCG------KPTSSTPKLDQLLYNCKNKKCDFYLLI------------ENEKEKKKSEIDDDNVTK----CYCGKPAKLKTSLKNNANKGRQYYSCNQITNCKFFCW 699
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081353.1 (SMESG000081353.1) HSP 1 Score: 294.664 bits (753), Expect = 7.950e-90 Identity = 149/266 (56.02%), Postives = 189/266 (71.05%), Query Frame = 3
Query: 123 FATRLLSEGIHPHNGKKSDQAHPPIHPLKQGIGLNEDEARIFELITRHFLACLSKDALGHETKVLIDCISKAYSADSWPVRGNIRLRSEIGEGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGIGEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKEGVAKYL 920
FA +L +GI P G K+D AHPPI PLK GIGL DE +FELITRHFLACLSKDA T++LIDCISK + D+W + + S +GE FSLTG V E+NYLDVYPY+K +D +P Y IG+IFEP+ V I +T PS LT+ DL+ LMDK+GIGTDATHAEHI K+K R+Y P NF +P KLG+GL++ Y+ +G +++P LRS+FEADL+ ICNGTK P EVL HYL+ YK I+ + I+KL++ V KYL
Sbjct: 310 FAKNILLDGITPMCGNKNDNAHPPIIPLKLGIGLAFDENHLFELITRHFLACLSKDAQCSRTEILIDCISKDHGRDTWKHKDSKIACSGLGEEFSLTGTIVREKNYLDVYPYEKLLDTFIPNYEIGDIFEPSCVGIKMGKTCPPSFLTDVDLISLMDKYGIGTDATHAEHIYKIKVREYTCLKYP--NFTVPTKLGIGLIDAYNYLGINIAQPMLRSKFEADLKEICNGTKDPNEVLRHYLDEYKSIFLESIKNIDKLQDIVLKYL 573 HSP 2 Score: 64.3142 bits (155), Expect = 1.883e-10 Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 2
Query: 2 LWLGCDREGENIGMEIVDTCLAIKPNITVKRAKFSEITFQVRHKAV 139
+W CDREGENIGMEIVD CLA+ I VKRA+FSEIT + +A+
Sbjct: 62 IWTDCDREGENIGMEIVDVCLAVNSKIDVKRARFSEITAESVTRAI 107 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of DNA topoisomerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of DNA topoisomerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of DNA topoisomerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008509 ID=SMED30008509|Name=DNA topoisomerase|organism=Schmidtea mediterranea sexual|type=transcript|length=2104bpback to top protein sequence of SMED30008509-orf-1 >SMED30008509-orf-1 ID=SMED30008509-orf-1|Name=SMED30008509-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=497bp MDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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