G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30008484
Length (bp)1336
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008484) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008484) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008484) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008484) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008484

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30008484h1SMcG0022110 dd_Smed_v4_13762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008484h1SMcG0022110 dd_Smed_v4_13762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30008484h1SMcG0022110 dd_Smed_v4_26560_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008484h1SMcG0022110 dd_Smed_v4_26560_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30008484h1SMcG0022110 dd_Smed_v4_26560_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30008484h1SMcG0022110 dd_Smed_v4_26560_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008484h1SMcG0022110 dd_Smed_v6_13762_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A193KUC7 (GCR117 OS=Schmidtea mediterranea OX=79327 GN=gcr117 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 4.444e-166
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 1
Query:  271 MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIXXXXXXXXXXXXXXXXXXXXXXXXXXXXQREXXXXXXXXXXXXXXXXXXXPNTCLFFIMKSLQNDSTINQNYLQ 1194
            MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQNDSTINQNYLQ
Sbjct:    1 MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQNDSTINQNYLQ 308          

HSP 2 Score: 90.8929 bits (224), Expect = 2.928e-16
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 3
Query: 1197 LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS 1334
            LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS
Sbjct:  310 LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS 355          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183SIW2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4160 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 5.850e-39
Identity = 90/224 (40.18%), Postives = 135/224 (60.27%), Query Frame = 1
Query:  298 NYSYIDSPYDMLYLC-DPKTENINI--NCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFT 960
            N+ Y+DSP+D+L  C D +T N      C +S ILG +S YIFP VG  GVVSN  + Y+FLF + + +RQ I L  +A+AD++ I++FGW+W+FPAKGLPY T+G +YFF++N + + C VFR +Y FTS ++ ++FL+   DRC  +YFP +  R++ K  +    +  L C  L + Y    R         C +   +K     L +Y VL+ +S +L T
Sbjct:    8 NWLYVDSPWDLLRDCKDYQTGNSTKFGACVLSHILGITSAYIFPIVGTFGVVSNFLVFYVFLFVFRRSSRQMIYLGCVAIADMMTIVSFGWMWMFPAKGLPYATSGRVYFFVLNVNDLSCKVFRYVYSFTSCLSSSLFLVTACDRCLCVYFPLKFSRLSKKRAWQAVGLTILCCSLLMLPYPILARHDKFQGKMICWVHEGEK----VLQIYHVLLANSSLLQT 227          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3SM37 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS6000 PE=4 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 8.864e-39
Identity = 92/231 (39.83%), Postives = 137/231 (59.31%), Query Frame = 1
Query:  298 NYSYIDSPYDMLYLCD---PKTENINI---NCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGII 972
            N+ Y+DSP+D+L  C        N +I    C +S +LGF S YIFPFVGF GV+SNI++ Y+FLF + K +RQ I L+ +A AD+  ++ FGW+W+FPAKGLPY T  ++YFFI N ++  C +FR LY FTS V+ +IFLL   DRC  IYFP +  RI+ K  +    +  L    + + +   I     +    C +  +  +    L +Y VL+ +S ++ T+ +I
Sbjct:   16 NWPYLDSPWDLLRDCQYLASGAANASIYRGACILSHMLGFVSAYIFPFVGFFGVLSNIFVTYVFLFVFRKPSRQMIYLACVAAADVATVILFGWIWMFPAKGLPYATGAHVYFFIFNVNNYSCKIFRYLYSFTSCVSSSIFLLTAFDRCLCIYFPLQFARISRKRAWEAVGVTTLLSALMMLPFGIMIGHGPSNGKIICWVQVEPNA----LQIYHVLLANSSLVQTILVI 242          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3T6L1 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS2698 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.258e-37
Identity = 88/224 (39.29%), Postives = 132/224 (58.93%), Query Frame = 1
Query:  298 NYSYIDSPYDMLYLC---DPKTENINI---NCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGV 951
            N++Y+DSP+D+L  C   +    N +I    C +S +LGF S +IFPFVG  GV+SNI++ Y+FLF + K +RQ I L+ +A AD+  I+ FGW+W+FP+KGLPY T  ++YFFI N S+  C +FR  Y FTS V+ +IFLL   DRC  IYFP +  RI+ K  +    +  L    L + +   +     +    C +  +  +    L +Y VL+ +S +
Sbjct:   16 NWAYLDSPWDLLRDCQYLNSGAANASIYRGACILSHMLGFVSAFIFPFVGVFGVISNIFVTYVFLFVFRKPSRQMIYLACVAAADVATIILFGWIWMFPSKGLPYATGAHVYFFIFNVSNYSCKIFRYFYSFTSCVSSSIFLLTAFDRCLCIYFPLQFARISRKRAWEAVGVTTLLSALLMLPFGIMVEHGPSNGKIICWVQVEANT----LQIYHVLLANSSL 235          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P6PGL7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS266 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 2.198e-34
Identity = 93/230 (40.43%), Postives = 135/230 (58.70%), Query Frame = 1
Query:  298 NYSYIDSPYDMLYLCDPKTENININ---CAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFI--RTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGII 972
            ++ YIDSP+D+   CD     +  N   C I+ +LG  S YIFPF+G  G++SN  + Y+F F Y K TRQ I L  +A AD+L ++ FGW+W+FPAKGLPY T   +YFFI N + V C VFR  Y FT++++ ++FLL  LDRC +IYFP R  RI+ +  +    I+ L    L +L  G +   ++ Q       +  K      FL +Y VL+ +S +L T+ +I
Sbjct:    8 DWPYIDSPWDLFLECDRYNSTVRKNFGACIIAHMLGIVSAYIFPFIGAFGLISNFLVLYVFFFVYRKPTRQMIYLGSIAGADILTLITFGWIWLFPAKGLPYATLARVYFFIFNFNDVTCKVFRYAYSFTASLSSSLFLLTALDRCLSIYFPLRFSRISTRRAWEAVLIITLVS-ALAMLPFGLMVGHSLSQGKIICWVLEQKT-----FLQIYHVLLSNSSLLQTLLVI 231          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000022744.1 (SMESG000022744.1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.236e-169
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 1
Query:  271 MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIXXXXXXXXXXXXXXXXXXXXXXXXXXXXQREXXXXXXXXXXXXXXXXXXXPNTCLFFIMKSLQNDSTINQNYLQ 1194
            MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQNDSTINQNYLQ
Sbjct:    1 MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQNDSTINQNYLQ 308          

HSP 2 Score: 90.8929 bits (224), Expect = 8.143e-20
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 3
Query: 1197 LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS 1334
            LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS
Sbjct:  310 LYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALILSNSTKNLSITS 355          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000055742.1 (SMESG000055742.1)

HSP 1 Score: 161.77 bits (408), Expect = 4.075e-45
Identity = 100/226 (44.25%), Postives = 134/226 (59.29%), Query Frame = 1
Query:  292 KLNYSYIDSPYDMLYLCDPKTENININ----CAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNK-DKSLNLFLNLYRVLIVHSGVL 954
            K NYSYID+PYD+LY C  + EN   +    C +S I G  S YIFP     G +SN+ ICYIF+ +Y K+TRQTILL+ L+ ADLL IL+ GWLW+FPAKGLPY + G IY FIIN S   C +FR    F+ +  F  FLLVCLDR  AIY+P + +    K       ++   C+  + +   F+R+I      FCT  +  D     FL  +R+L ++SG +
Sbjct:    8 KSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAI 233          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000022976.1 (SMESG000022976.1)

HSP 1 Score: 140.198 bits (352), Expect = 4.231e-37
Identity = 96/240 (40.00%), Postives = 133/240 (55.42%), Query Frame = 1
Query:  286 SKKLNYSYIDSPYDMLYLCDP-----KTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPK--RTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFI----RTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGII 972
             KK  + YIDSP+D+L  C+      + E  +  C  S ILG  SGYIFPFVG  G++ N  I Y+F+ RYP   RTRQ I LS LA+AD  +I+ FGWLWIFPAKGLPY++ G IY+F  N+++  C   R +Y F ST+   +FLL  +DRC +IY P   L+ + K      ++V +  +   +L T F       + +     C I   D    +   LY VL  + G + T+ II
Sbjct:    4 EKKNAWLYIDSPWDLLVPCEDLNTTIEDERTSALCISSHILGVFSGYIFPFVGVFGIIFNFMIFYVFMIRYPPKLRTRQLIYLSALAIADNFSIITFGWLWIFPAKGLPYISKGLIYYFTFNENNPTCKAHRYIYSFASTLAVNLFLLTSIDRCLSIYKP---LKYSKKGKTFAWTLVAITTVISGILMTPFAIIISLNVNKQNLIICWI--DDARFAVSGQLYHVLFSNCGFIQTIAII 238          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000053055.1 (SMESG000053055.1)

HSP 1 Score: 129.413 bits (324), Expect = 3.199e-33
Identity = 84/240 (35.00%), Postives = 127/240 (52.92%), Query Frame = 1
Query:  280 FTSKKLNYSYIDSPYDMLYLCDP-----KTENININCAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFL-TCSIV---FLFCLPLTVLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGII 972
            F+ +K  + Y+DSP+D+L  C+       T N  + C  S I+GF S YI P +   G+VSN+   YI   +   RTRQ I LS++A  D +NI+ +GW+WIF AKGLPY + G + FF  NQ  + C + R      S    +IFLL  +DR FAI++P + L I  +  +  +  ++   F+   P  + Y G+  T  Q    +  +   ++ L +FL +Y VL    G   T+ II
Sbjct:    6 FSEQKARWKYVDSPWDLLTACEELRLSKTTGNTLVFCIFSHIMGFWSAYILPIISLFGIVSNMVTIYILSSKLHDRTRQYIYLSFIAGCDSVNIICYGWIWIFFAKGLPYASLGKVSFFTYNQGEISCKLHRYFIAAISCSASSIFLLASVDRWFAIFWPLKCLNIPLRRAWQYSIGVIISSFILMSPFAI-YMGYTETGNQKTLCWIKVSTDEQGLAIFLQVYNVLFSFCGSFQTIAII 244          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000052989.1 (SMESG000052989.1)

HSP 1 Score: 125.946 bits (315), Expect = 4.360e-32
Identity = 87/244 (35.66%), Postives = 128/244 (52.46%), Query Frame = 1
Query:  265 PKMGYFTSKKLNYSYIDSPYDMLYLCDP-KTENININ----CAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTIXXXXXXXXXXXXXXXFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLT---VLYTGFIRTIYQHPYFFCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGII 972
            P + Y +  K  + +IDSP+D+L  C+  K +  N N    C  S+I+GF S ++FP +   G+  NI I YIF F++  +TRQ I LS+LA  D  N++  GW+WIF AKGLPY ++G +YFF  NQ  + C + R      S    +IFLL  +DR FAI++P + L I  K  +   S+V L    +    ++Y     T       +       ++L  FL +Y VL    G   T+ II
Sbjct:    2 PNLTY-SDLKAQWKFIDSPWDLLISCEEVKLKQTNENNYLFCVFSQIMGFWSAFVFPVISIFGIFLNILIIYIFNFKFRDKTRQYIYLSFLAAFDSFNLICSGWMWIFFAKGLPYASSGVLYFFTYNQGVIPCKLHRYFTAAISCSAISIFLLTSIDRYFAIFWPLKCLNIPLKKAWQYSSMVVLLSFIMMSPFIVYMDHSETGNHKTICWLEASKDKETLGTFLQIYNVLFSFCGSFQTLAII 244          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUC74.444e-166100.00GCR117 OS=Schmidtea mediterranea OX=79327 GN=gcr11... [more]
A0A183SIW25.850e-3940.18G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3SM378.864e-3939.83G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3T6L17.258e-3739.29G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P6PGL72.198e-3440.43G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022744.11.236e-169100.00SMESG000022744.1[more]
SMESG000055742.14.075e-4544.25SMESG000055742.1[more]
SMESG000022976.14.231e-3740.00SMESG000022976.1[more]
SMESG000053055.13.199e-3335.00SMESG000053055.1[more]
SMESG000052989.14.360e-3235.66SMESG000052989.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008484 ID=SMED30008484|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1336bp
AGCAATACATAGAACAAGAGGAAAGAGGGAGTTATCGTACTTTTGGACAG
TCAGACACGATAGAATGATTTTTACGTATAATAAACCAAATACAAAAAAC
TTTGCTTGACTTGAATATTAGCACAATTCGTTGAAACCGGCAAGATTGGC
TGTTGACAAATCAAAATTGGGGCAACAAATAATTTTAACCAATAACAAAT
CACAATGTCAAAATGATATTTACCAAAATCAAATCAAATTTATCTCACGT
TTAAAACCTCCAAACCAAAAATGGGATATTTTACTTCGAAAAAGTTAAAT
TATTCTTACATTGACTCCCCCTATGACATGCTTTATTTATGTGATCCCAA
AACTGAAAATATCAATATAAATTGTGCAATTAGTGAAATCCTCGGATTTT
CATCGGGATATATTTTCCCATTTGTTGGCTTTATCGGTGTCGTAAGCAAT
ATTTATATTTGTTATATCTTTCTTTTCAGATACCCAAAACGAACAAGACA
GACGATTTTGTTGTCATACTTGGCGTTAGCGGACCTTTTGAACATTCTAG
CATTCGGTTGGTTGTGGATATTTCCTGCTAAAGGATTACCATATATGACT
AATGGGAATATTTACTTTTTTATTATAAATCAATCAAGCGTTTACTGTAC
AGTTTTTCGAACTTTGTACTTTTTTACCTCAACTGTCACATTTGCTATTT
TTCTTTTAGTTTGCCTTGACAGATGTTTTGCCATTTATTTCCCATTTAGA
TCGTTGAGAATCACAAACAAAATGACATTCTTAACCTGCTCGATAGTATT
TCTTTTTTGTCTTCCTCTAACGGTTTTGTACACGGGTTTTATTCGAACAA
TTTACCAACATCCGTATTTTTTCTGTACAATCGGAAACAAAGATAAAAGC
CTGAATTTGTTCCTGAATTTATATCGAGTTTTGATTGTTCATTCAGGAGT
TCTTTTCACCGTTGGAATAATCATATCGAATCTTGCGTTGATCTTGAAAC
TTGTTCTTTTATCTAAAACAAACTTACTGTTGATTAAAACAAAATCCAAT
AATATTCAGAGAGAAATTCGTTCAACAATTACCATTCTAATTTTATCGCT
AATTTTTCTCAGTGGATCCTTGCCAAATACTTGTTTATTTTTCATCATGA
AATCCTTACAAAATGACAGCACTATAAATCAGAACTATTTACAGTCCTTT
ACTCCCTAACTGATGCGTTCTACCAACTACTGATCTGGATTGAGTCTTTC
AGTTTTTTGGTTTACGTCATCAGGATGAAAAACTTTAGAGCACTGATTCT
TTCCAATTCTACAAAAAACCTTTCTATAACGTCAAC
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protein sequence of SMED30008484-orf-1

>SMED30008484-orf-1 ID=SMED30008484-orf-1|Name=SMED30008484-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=313bp
MGYFTSKKLNYSYIDSPYDMLYLCDPKTENININCAISEILGFSSGYIFP
FVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWI
FPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLD
RCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYF
FCTIGNKDKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKT
NLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQNDS
TINQNYLQSFTP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 60..307
e-value: 2.7E-9
score: 36.7
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 43..313
e-value: 3.3E-22
score: 80.9
NoneNo IPR availablePANTHERPTHR24246:SF27ADENOSINE RECEPTOR, ISOFORM Acoord: 46..310
NoneNo IPR availablePANTHERPTHR24246OLFACTORY RECEPTOR AND ADENOSINE RECEPTORcoord: 46..310
NoneNo IPR availableCDDcd006377tm_classA_rhodopsin-likecoord: 48..313
e-value: 2.77319E-13
score: 66.5322
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 28..300
NoneNo IPR availableTMHMMTMhelixcoord: 81..103
NoneNo IPR availableTMHMMTMhelixcoord: 271..293
NoneNo IPR availableTMHMMTMhelixcoord: 127..149
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 220..242
NoneNo IPR availableTMHMMTMhelixcoord: 169..191
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 59..313
score: 18.325