SMED30008453

Overview
NameSMED30008453
Smed IDSMED30008453
Length (bp)1985
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008453 (SMED30008453) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008453 (SMED30008453) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008453 (SMED30008453) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008453 (SMED30008453) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008453

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008453h1SMcG0000989 SmedASXL_015816SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30008453h1SMcG0000989 Contig42758newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008453h1SMcG0000989 Contig42758uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30008453h1SMcG0000989 dd_Smed_v4_9776_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008453h1SMcG0000989 dd_Smed_v4_9776_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008453h1SMcG0000989 dd_Smed_v4_9776_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008453h1SMcG0000989 dd_Smed_v6_2867_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30008453h1SMcG0000989 DN293934smed_ncbi_20240424PMID:30399335
Ross et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30008453h1SMcG0000989 DN293934smed_ncbi_20240424PMID:30399335
Ross et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30008453h1SMcG0000989 dd_Smed_v4_9776_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30008453h1SMcG0000989 dd_Smed_v4_9776_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30008453h1SMcG0000989 dd_Smed_v4_2867_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30008453 vs. TrEMBL
Match: A0A4W6BNI5 (Mindbomb E3 ubiquitin protein ligase 2 OS=Lates calcarifer OX=8187 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 5.938e-6
Identity = 92/372 (24.73%), Postives = 157/372 (42.20%), Query Frame = 3 
Query:  702 GNVSRMKEYVQKYESVI---SQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFAD-DLEKKLH------NANIRQVLTNKLKRNSENIELSKNEF---MEILMDRTFQYTDKLLERLSKDF-LSENISKEIIEQAIASNKIKLLTSLFHKANVDV--QNKVFEDILFCYALNANEIDFETL--QNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIF---QKMNDSTDKSLKNRMMSVAKSIVNI----GGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSI 1742
            G+ S+++E VQKY   +   +Q K   QV  H+               IE V AL     +  N      D D +  LH       A I ++L +K      N+ L  N     + I +++ F    ++L   S D  L ++     +  AIA +   ++  L    N+D   QN    ++L   AL  N++  E +  +   LV  K  ++G S L     +N       L+    C+  +N++N++ Q+PLQ+A+     D+    + E + +N EDE+G TA+       Q  N S    L N  +SV  ++       G DI     +G+  +D+  +S     I+++ +     R  Q    +ICS+
Sbjct:  485 GSASKVRELVQKYPDKVDIKNQGKTALQVAAHQGH-------------IEVVKAL-----LQANSSIEVKDEDGDTALHYTAFGNQAEIARLLLSK----GANVNLLNNSMCTALHIAVNKGFTDVVRVLTEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILVVVPNIDFTQQNHRGFNLLHHAALKGNKLATEKILARARQLVDVK-KDDGFSALHLAALNNHRDVAEILIKEGRCD--VNIRNNRNQTPLQLAVTQGHTDLVQLLVAEGADVNMEDEDGDTAMHVALLRPQLANLSASGLLGNTELSVGAAMACFLAQEGADINYANHKGKSPLDLVADSTMLQLIKSFSEKH---RYLQHMFCQICSM 828
[Close]
BLAST of SMED30008453 vs. Ensembl Cavefish
Match: wasa (wiskott-Aldrich syndrome protein-like [Source:NCBI gene;Acc:103022907])

HSP 1 Score: 58.151 bits (139), Expect = 2.431e-8
Identity = 32/114 (28.07%), Postives = 62/114 (54.39%), Query Frame = 3 
Query:   93 SNILNLNEYEQVLNTLKNKCKILALALGKLNFNMD---FNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENF 425
            S +L+L E E+V + L  +C  L  A+ +L   +    FN+    + +L   +D+ +R+Y IR + +++   L + E+ ++  YH   +  H+F +D   + LNF D  ++E+F
Sbjct:   15 SALLSLQENEKVQDLLGRRCVSLCTAVAQLFMALPHSPFNWSLQHSGVLCFVKDNPQRSYFIRLYDIKAGKMLWEQELYNQLTYHRARAFFHTFPSDDCQVGLNFADEQEAESF 128
[Close]
BLAST of SMED30008453 vs. Planmine SMEST
Match: SMESG000074789.1 (SMESG000074789.1)

HSP 1 Score: 1134.01 bits (2932), Expect = 0.000e+0
Identity = 586/640 (91.56%), Postives = 590/640 (92.19%), Query Frame = 3 
Query:   84 MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGXXXXXXXXXXXXXXXXXXXHQIDNVNTNKSQTPNSIFYNLAIKDN-----------------------------------------------MSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLKRNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 1862
            MDLSNILNLNEYEQVLNTLKNKCKILALALGK NFNMDFNFESS+ASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIK+KHQIDNVNTNKSQTPNSIFYNLAIKDN                                               MSENDAAVELFDVAPPPTESPPPIK RLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKL+RNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEI+FETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTD SLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI
Sbjct:    1 MDLSNILNLNEYEQVLNTLKNKCKILALALGKFNFNMDFNFESSEASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKNKHQIDNVNTNKSQTPNSIFYNLAIKDNVSENDAAVKILDVAAPTESPPPIKPRLSKVKGTDKVKLQSQTIDLDNMSENDAAVELFDVAPPPTESPPPIKHRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLERNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIEFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDNSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 640
[Close]
BLAST of SMED30008453 vs. Planmine SMEST
Match: SMESG000074789.1 (SMESG000074789.1)

HSP 1 Score: 1126.69 bits (2913), Expect = 0.000e+0
Identity = 586/593 (98.82%), Postives = 590/593 (99.49%), Query Frame = 3 
Query:   84 MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGXXXXXXXXXXXXXXXXXXXHQIDNVNTNKSQTPNSIFYNLAIKDNMSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLKRNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 1862
            MDLSNILNLNEYEQVLNTLKNKCKILALALGK NFNMDFNFESS+ASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIK+KHQIDNVNTNKSQTPNSIFYNLAIKDNMSENDAAVELFDVAPPPTESPPPIK RLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKL+RNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEI+FETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTD SLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI
Sbjct:    1 MDLSNILNLNEYEQVLNTLKNKCKILALALGKFNFNMDFNFESSEASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKNKHQIDNVNTNKSQTPNSIFYNLAIKDNMSENDAAVELFDVAPPPTESPPPIKHRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLERNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIEFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDNSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 593
[Close]
BLAST of SMED30008453 vs. Planmine SMEST
Match: SMESG000074789.1 (SMESG000074789.1)

HSP 1 Score: 1121.69 bits (2900), Expect = 0.000e+0
Identity = 585/656 (89.18%), Postives = 590/656 (89.94%), Query Frame = 3 
Query:   84 MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGXXXXXXXXXXXXXXXXXXXHQIDNVNTNKSQTPNSIFYNLAIKDN-----------------------------------------------MSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIR----------------QVLTNKLKRNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 1862
            MDLSNILNLNEYEQVLNTLKNKCKILALALGK NFNMDFNFESS+ASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIK+KHQIDNVNTNKSQTPNSIFYNLAIKDN                                               MSENDAAVELFDVAPPPTESPPPIK RLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIR                +VLTNKL+RNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEI+FETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTD SLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI
Sbjct:    1 MDLSNILNLNEYEQVLNTLKNKCKILALALGKFNFNMDFNFESSEASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKNKHQIDNVNTNKSQTPNSIFYNLAIKDNVSENDAAVKILDVAAPTESPPPIKPRLSKVKGTDKVKLQSQTIDLDNMSENDAAVELFDVAPPPTESPPPIKHRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRHLLISNYLGRITNLEDRVLTNKLERNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIEFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDNSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKI 656
[Close]
BLAST of SMED30008453 vs. Planmine SMEST
Match: SMESG000074789.1 (SMESG000074789.1)

HSP 1 Score: 1110.52 bits (2871), Expect = 0.000e+0
Identity = 574/631 (90.97%), Postives = 579/631 (91.76%), Query Frame = 3 
Query:   84 MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGXXXXXXXXXXXXXXXXXXXHQIDNVNTNKSQTPNSIFYNLAIKDN-----------------------------------------------MSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLKRNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCR 1835
            MDLSNILNLNEYEQVLNTLKNKCKILALALGK NFNMDFNFESS+ASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIK+KHQIDNVNTNKSQTPNSIFYNLAIKDN                                               MSENDAAVELFDVAPPPTESPPPIK RLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKL+RNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEI+FETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTD SLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCP  +
Sbjct:    1 MDLSNILNLNEYEQVLNTLKNKCKILALALGKFNFNMDFNFESSEASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKNKHQIDNVNTNKSQTPNSIFYNLAIKDNVSENDAAVKILDVAAPTESPPPIKPRLSKVKGTDKVKLQSQTIDLDNMSENDAAVELFDVAPPPTESPPPIKHRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLERNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIEFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDNSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPTVK 631
[Close]
BLAST of SMED30008453 vs. Planmine SMEST
Match: SMESG000074789.1 (SMESG000074789.1)

HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0
Identity = 573/647 (88.56%), Postives = 579/647 (89.49%), Query Frame = 3 
Query:   84 MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGXXXXXXXXXXXXXXXXXXXHQIDNVNTNKSQTPNSIFYNLAIKDN-----------------------------------------------MSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIR----------------QVLTNKLKRNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCR 1835
            MDLSNILNLNEYEQVLNTLKNKCKILALALGK NFNMDFNFESS+ASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIK+KHQIDNVNTNKSQTPNSIFYNLAIKDN                                               MSENDAAVELFDVAPPPTESPPPIK RLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIR                +VLTNKL+RNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEI+FETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTD SLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCP  +
Sbjct:    1 MDLSNILNLNEYEQVLNTLKNKCKILALALGKFNFNMDFNFESSEASLLLLTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFILYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKNKHQIDNVNTNKSQTPNSIFYNLAIKDNVSENDAAVKILDVAAPTESPPPIKPRLSKVKGTDKVKLQSQTIDLDNMSENDAAVELFDVAPPPTESPPPIKHRLSKVSETDKAKLQSKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSETIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRHLLISNYLGRITNLEDRVLTNKLERNSENIELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIKLLTSLFHKANVDVQNKVFEDILFCYALNANEIEFETLQNHNLVSAKIFENGESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSLDIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDNSLKNRMMSVAKSIVNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKICSICRDNEVDCVYNCGHTFCMTCGETMYCCPTVK 647
The following BLAST results are available for this feature:
BLAST of SMED30008453 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A4W6BNI55.938e-624.73Mindbomb E3 ubiquitin protein ligase 2 OS=Lates ca... [more]
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BLAST of SMED30008453 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wasa2.431e-828.07wiskott-Aldrich syndrome protein-like [Source:NCBI... [more]
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BLAST of SMED30008453 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008453 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000074789.10.000e+091.56SMESG000074789.1[more]
SMESG000074789.10.000e+098.82SMESG000074789.1[more]
SMESG000074789.10.000e+089.18SMESG000074789.1[more]
SMESG000074789.10.000e+090.97SMESG000074789.1[more]
SMESG000074789.10.000e+088.56SMESG000074789.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008453 ID=SMED30008453|Name=SMED30008453|organism=Schmidtea mediterranea sexual|type=transcript|length=1985bp
GTTAAATTAAACACATTAATCAAGGAATCATATTTTGATGAAATTCAAAT
ATTTTTAAAATTAAATATTTTGAAATTTATATTATGGATTTATCGAATAT
TTTAAATCTTAATGAATATGAACAAGTGTTAAATACTTTGAAAAATAAAT
GCAAAATTTTAGCGTTGGCCCTTGGAAAACTTAACTTCAATATGGATTTT
AATTTTGAATCTTCAGATGCTTCATTACTTCTGCTAACTGAAGATGATGA
TGAAAGAAATTACCACATCAGATCATTTAAATTGGAAAGTTTTAATCAAC
TTCAGCAATACGAGATTTTATCCGAATCTAATTATCATATAATCTGTTCT
GAGTTGCATTCATTCGTGAATGATAAATTTATTTTGTATCTCAACTTTTT
GGATTTCATTCAATCCGAAAATTTTGGAAATAAAGTTAAAAATGTGATAG
AAAAGAAAAAAGAAAAAAGTGAAATCAAGAGCAAACATCAAATAGACAAC
GTTAATACGAATAAATCTCAAACCCCCAATTCGATATTTTATAATCTTGC
TATAAAAGATAATATGAGTGAAAATGATGCTGCTGTTGAATTATTTGATG
TTGCTCCTCCTCCAACAGAATCTCCGCCACCTATAAAACAACGTTTGTCA
AAGGTCAGTGAAACAGATAAAGCCAAATTACAATCAAAAACAATTGACCT
AGGTAACGTGAGTAGAATGAAAGAATATGTACAGAAATATGAATCTGTTA
TTTCTCAAACGAAAAATCCTCCACAAGTACAGTTACATAAGCCACAACTC
ATTAGACGAAATACAACCAAATGTCATTCAGAGACAATTGAACAGGTTGA
TGCACTAGATGGACCAAGATTGATAAATCGAAATTTTAAAAGATCTTTTG
CAGATGATCTAGAAAAGAAACTACATAATGCGAACATTCGGCAGGTCTTG
ACAAATAAATTAAAAAGAAATTCTGAAAATATTGAGTTATCAAAGAATGA
ATTTATGGAAATTTTAATGGATCGCACCTTTCAATACACTGATAAATTGT
TAGAAAGATTGTCGAAAGATTTTCTTTCTGAAAATATTTCAAAAGAAATC
ATAGAGCAAGCAATCGCTTCAAATAAAATCAAGTTATTAACATCATTATT
TCACAAGGCTAATGTTGATGTTCAAAACAAAGTTTTCGAAGACATTCTAT
TCTGTTACGCTCTCAATGCCAATGAAATCGATTTTGAAACTCTTCAAAAT
CATAATTTAGTGAGTGCAAAAATATTCGAAAATGGCGAAAGTCTTTTAAC
CAATTTAATAAAAAGTAATAAACCGAAATTTGTATGGAAATTGCTGAGTT
CAAATAATTGCAATTTATACATCAATTTAAAAAATTCCAAATTGCAATCA
CCTTTACAAGTTGCGATTGAGATGAATTCATTGGATATCGCAAACTTCTT
TATATATGAATCCAGCCAATTAAATGACGAGGACGAGAATGGAATGACTG
CGTTGGAACAGATTTTTCAGAAAATGAATGATTCTACTGATAAATCTTTG
AAAAATAGAATGATGTCAGTTGCGAAGTCGATTGTAAATATAGGAGGAGA
CATTTACAAACTAACTTCCAGAGGTCGCTTATTAATTGATATATATAGAA
ACTCTTGGTTCGGGAATGAAATTGAAAACTATTATAAAAATAAAGGTCGT
ATTAGAAATTCACAGGAATCGGATATAAAAATATGCTCAATATGCAGAGA
TAATGAAGTTGATTGCGTGTACAATTGCGGTCACACATTTTGCATGACGT
GTGGAGAGACCATGTACTGCTGTCCAGTGTGTAGGTCTGAACCGACTAAT
CCCAGGAAAATTTTTCTCTGAAATTATTACTAAGTTTTTAACAATTTATA
CATGATTTAATCTTTTGCCATGTTATTTATAAATTATCAGTAGATTTGTA
TTAACTCTCTAGTTCTCAGGCCCATTTTGAGAACG
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protein sequence of SMED30008453-orf-1

>SMED30008453-orf-1 ID=SMED30008453-orf-1|Name=SMED30008453-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=596bp
MDLSNILNLNEYEQVLNTLKNKCKILALALGKLNFNMDFNFESSDASLLL
LTEDDDERNYHIRSFKLESFNQLQQYEILSESNYHIICSELHSFVNDKFI
LYLNFLDFIQSENFGNKVKNVIEKKKEKSEIKSKHQIDNVNTNKSQTPNS
IFYNLAIKDNMSENDAAVELFDVAPPPTESPPPIKQRLSKVSETDKAKLQ
SKTIDLGNVSRMKEYVQKYESVISQTKNPPQVQLHKPQLIRRNTTKCHSE
TIEQVDALDGPRLINRNFKRSFADDLEKKLHNANIRQVLTNKLKRNSENI
ELSKNEFMEILMDRTFQYTDKLLERLSKDFLSENISKEIIEQAIASNKIK
LLTSLFHKANVDVQNKVFEDILFCYALNANEIDFETLQNHNLVSAKIFEN
GESLLTNLIKSNKPKFVWKLLSSNNCNLYINLKNSKLQSPLQVAIEMNSL
DIANFFIYESSQLNDEDENGMTALEQIFQKMNDSTDKSLKNRMMSVAKSI
VNIGGDIYKLTSRGRLLIDIYRNSWFGNEIENYYKNKGRIRNSQESDIKI
CSICRDNEVDCVYNCGHTFCMTCGETMYCCPVCRSEPTNPRKIFL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR011993PH-like_dom_sf
IPR013083Znf_RING/FYVE/PHD
IPR020683Ankyrin_rpt-contain_dom
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 9..29
NoneNo IPR availableCOILSCoilCoilcoord: 286..306
NoneNo IPR availablePANTHERPTHR45751FAMILY NOT NAMEDcoord: 466..588
NoneNo IPR availablePANTHERPTHR45751:SF14coord: 466..588
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 550..589
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 2..134
e-value: 6.4E-9
score: 37.4
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 324..526
e-value: 1.4E-10
score: 42.3
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 336..519
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 528..595
e-value: 6.9E-10
score: 40.3
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 400..474
score: 9.808
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 551..584
score: 11.825