Aldo_ket_red domain-containing protein

Overview
NameAldo_ket_red domain-containing protein
Smed IDSMED30008411
Length (bp)1272
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Aldo_ket_red domain-containing protein (SMED30008411) t-SNE clustered cells

Violin plots show distribution of expression levels for Aldo_ket_red domain-containing protein (SMED30008411) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Aldo_ket_red domain-containing protein (SMED30008411) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Aldo_ket_red domain-containing protein (SMED30008411) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30008411

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30008411SMESG000053228.1 Contig5506GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30008411SMESG000053228.1 dd_Smed_v4_487_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008411SMESG000053228.1 dd_Smed_v4_487_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008411SMESG000053228.1 dd_Smed_v4_487_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30008411SMESG000053228.1 dd_Smed_v4_487_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30008411SMESG000053228.1 dd_Smed_v6_487_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008411SMESG000053228.1 dd_Smed_v4_487_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Human
Match: GCLM (glutamate-cysteine ligase modifier subunit [Source:HGNC Symbol;Acc:HGNC:4312])

HSP 1 Score: 108.227 bits (269), Expect = 1.913e-26
Identity = 59/183 (32.24%), Postives = 102/183 (55.74%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+II+  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I      P W+ RYS++ K+R +I+SKGYI
Sbjct:   62 DEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYI 244
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Human
Match: GCLM (glutamate-cysteine ligase modifier subunit [Source:HGNC Symbol;Acc:HGNC:4312])

HSP 1 Score: 108.227 bits (269), Expect = 3.473e-26
Identity = 59/183 (32.24%), Postives = 102/183 (55.74%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+II+  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I      P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Celegans
Match: E01A2.1 (pep chromosome:WBcel235:I:4140649:4142367:1 gene:WBGene00017084.1 transcript:E01A2.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E01A2.1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.190e-18
Identity = 54/199 (27.14%), Postives = 103/199 (51.76%), Query Frame = 2 
Query:  224 DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGD--------VYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKT------RLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYISKVF 778
            +R ++++ +K+FL   + + I + +  + + L+  +++ LI+S     +       D        V  L++ +E+ V+   I  +GV DF+  QL+ +    ++KPSIN +     C V   L+  A D+ +QLL H+D      +N+FKT        Q+ + + + E+   +W+ RYS+  + RS++ SKGYI + F
Sbjct:   77 ERDEIKITMKVFLSAWDVQQIDQAVTSLKEQLKTKDIEVLILSFPELDLIDGESEEDENNRWFEKVKPLYTYMEKLVETSEIASIGVSDFSARQLKTIIDHFDVKPSINHVRLDGCCQVPPELQALANDNDVQLLVHNDPTPFPTNNIFKTFCEIDSGSQKAVCSPLFET---TWLSRYSVWVRKRSIMTSKGYIVQFF 272
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Celegans
Match: E01A2.1 (pep chromosome:WBcel235:I:4139514:4142335:1 gene:WBGene00017084.1 transcript:E01A2.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E01A2.1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.230e-18
Identity = 54/199 (27.14%), Postives = 103/199 (51.76%), Query Frame = 2 
Query:  224 DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGD--------VYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKT------RLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYISKVF 778
            +R ++++ +K+FL   + + I + +  + + L+  +++ LI+S     +       D        V  L++ +E+ V+   I  +GV DF+  QL+ +    ++KPSIN +     C V   L+  A D+ +QLL H+D      +N+FKT        Q+ + + + E+   +W+ RYS+  + RS++ SKGYI + F
Sbjct:   91 ERDEIKITMKVFLSAWDVQQIDQAVTSLKEQLKTKDIEVLILSFPELDLIDGESEEDENNRWFEKVKPLYTYMEKLVETSEIASIGVSDFSARQLKTIIDHFDVKPSINHVRLDGCCQVPPELQALANDNDVQLLVHNDPTPFPTNNIFKTFCEIDSGSQKAVCSPLFET---TWLSRYSVWVRKRSIMTSKGYIVQFF 286
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Fly
Match: Gclm (gene:FBgn0046114 transcript:FBtr0334605)

HSP 1 Score: 83.5741 bits (205), Expect = 1.011e-17
Identity = 49/213 (23.00%), Postives = 96/213 (45.07%), Query Frame = 2 
Query:  212 LILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSI----------------------------CMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            L  + R+++ +  K+FL  ++ E +++ + ++   L    +D+++++  P  + ++                              + ++ EL+  LE+Y    +I QLG+ D     LE++  S ++ P+I Q+     C V   L++F   H IQL +H D   +LP   F      ++P   I+     W  RY +  + R V+ +KGYI
Sbjct:   74 LTENGRNEISIGAKIFLNRHSTESVNQAVEELLHILSVTHVDNVVLAYHPNAVATATPVATTKPPCSEDSNVSRATNWSQRNGKEGVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDG----LVPGYTID-----WTLRYQVHVRCRGVLTAKGYI 277
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Fly
Match: Gclm (gene:FBgn0046114 transcript:FBtr0084336)

HSP 1 Score: 83.5741 bits (205), Expect = 1.011e-17
Identity = 49/213 (23.00%), Postives = 96/213 (45.07%), Query Frame = 2 
Query:  212 LILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSI----------------------------CMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            L  + R+++ +  K+FL  ++ E +++ + ++   L    +D+++++  P  + ++                              + ++ EL+  LE+Y    +I QLG+ D     LE++  S ++ P+I Q+     C V   L++F   H IQL +H D   +LP   F      ++P   I+     W  RY +  + R V+ +KGYI
Sbjct:   74 LTENGRNEISIGAKIFLNRHSTESVNQAVEELLHILSVTHVDNVVLAYHPNAVATATPVATTKPPCSEDSNVSRATNWSQRNGKEGVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDG----LVPGYTID-----WTLRYQVHVRCRGVLTAKGYI 277
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Zebrafish
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:ZFIN;Acc:ZDB-GENE-030131-5906])

HSP 1 Score: 100.138 bits (248), Expect = 1.566e-23
Identity = 58/189 (30.69%), Postives = 98/189 (51.85%), Query Frame = 2 
Query:  200 NHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            N + +  ++R +L+V+VKLFL   +   I   +      L  ++LDS+II+        S  +  +  LW +LE  V+  +I  +G  D     LE ++   +IKPS NQ+     C +   L  FA +  IQLL+H D  +++ +  F+  +Q     + ++     WV RYSI+ K+R +I++KGYI
Sbjct:   76 NTEAITPEEREELKVSVKLFLTEWDCSSIRSAVDMACLSLGVSQLDSVIIAPPSLPEGESQTLTHLQPLWQELESLVQSQKIAAIGTSDLDKTLLEQLYNWAQIKPSSNQVNLASCCVMPPDLTAFAKEFDIQLLTHSDPKELISAAGFQEAVQGSSQELQVDDWRLEWVLRYSIIVKSRGIIKAKGYI 264
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Xenopus
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:NCBI gene;Acc:549290])

HSP 1 Score: 108.612 bits (270), Expect = 2.475e-26
Identity = 53/183 (28.96%), Postives = 101/183 (55.19%), Query Frame = 2 
Query:  218 LDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            LD+R +++V+VKLF+++ ++  +++ +      L   ++DS+II+  P     S  + ++   W +LE  V++ +++ +G  D     LE ++   ++KP+ NQ+     C +   L +FA    IQLL+H+D  ++L    F+  L+E        +  P W+ RYS++ K R +I+ KGYI
Sbjct:   83 LDEREEMKVSVKLFIVSPSHSSVTQAIDMACSTLGVAQIDSMIIAPPPLEDGRSFSLENLQPYWEELESLVRNGKVVSIGTSDLDKALLEQLYLWSQVKPASNQVNLASCCIMPPDLTEFAKQFDIQLLTHNDPKELLSEEAFQEALKESAQECHSSAWSPIWILRYSVIVKTRGIIKLKGYI 265
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Xenopus
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:NCBI gene;Acc:549290])

HSP 1 Score: 88.1965 bits (217), Expect = 2.864e-19
Identity = 45/150 (30.00%), Postives = 79/150 (52.67%), Query Frame = 2 
Query:  317 LECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            L   ++DS+II+  P     S  + ++   W +LE  V++ +++ +G  D     LE ++   ++KP+ NQ+     C +   L +FA    IQLL+H+D  ++L    F+  L+E        +  P W+ RYS++ K R +I+ KGYI
Sbjct:   97 LGVAQIDSMIIAPPPLEDGRSFSLENLQPYWEELESLVRNGKVVSIGTSDLDKALLEQLYLWSQVKPASNQVNLASCCIMPPDLTEFAKQFDIQLLTHNDPKELLSEEAFQEALKESAQECHSSAWSPIWILRYSVIVKTRGIIKLKGYI 246
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Mouse
Match: Gclm (glutamate-cysteine ligase, modifier subunit [Source:MGI Symbol;Acc:MGI:104995])

HSP 1 Score: 107.842 bits (268), Expect = 3.454e-26
Identity = 58/183 (31.69%), Postives = 103/183 (56.28%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+I++  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I  +   P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Mouse
Match: Gclm (glutamate-cysteine ligase, modifier subunit [Source:MGI Symbol;Acc:MGI:104995])

HSP 1 Score: 107.842 bits (268), Expect = 3.454e-26
Identity = 58/183 (31.69%), Postives = 103/183 (56.28%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+I++  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I  +   P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Mouse
Match: Gclm (glutamate-cysteine ligase, modifier subunit [Source:MGI Symbol;Acc:MGI:104995])

HSP 1 Score: 67.3958 bits (163), Expect = 1.367e-12
Identity = 41/147 (27.89%), Postives = 77/147 (52.38%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRL 658
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+I++  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D      + +F +RL
Sbjct:   53 DEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKG--ETGIFISRL 197
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Match: sp|P48508|GSH0_RAT (Glutamate--cysteine ligase regulatory subunit OS=Rattus norvegicus OX=10116 GN=Gclm PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.240e-27
Identity = 58/183 (31.69%), Postives = 104/183 (56.83%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    +++   +    L   +LDS+I++  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I  +   P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2T9Y6|GSH0_BOVIN (Glutamate--cysteine ligase regulatory subunit OS=Bos taurus OX=9913 GN=GCLM PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.820e-27
Identity = 58/183 (31.69%), Postives = 103/183 (56.28%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    +++   +    L   +LDS+II+  P     ++ +  +   W +L+  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I      P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIIASPPVEDGVNLSLEHLQPYWEELQNLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIRAHEWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Match: sp|P48507|GSH0_HUMAN (Glutamate--cysteine ligase regulatory subunit OS=Homo sapiens OX=9606 GN=GCLM PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.668e-25
Identity = 59/183 (32.24%), Postives = 102/183 (55.74%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+II+  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I      P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Match: sp|O09172|GSH0_MOUSE (Glutamate--cysteine ligase regulatory subunit OS=Mus musculus OX=10090 GN=Gclm PE=1 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.416e-25
Identity = 58/183 (31.69%), Postives = 103/183 (56.28%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIF-KYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            D+R +++V+ KLF++ +N    ++    +    L   +LDS+I++  P     ++ +  +   W +LE  V+  +I+ +G  D    QLE ++Q  ++KP+ NQ+     C +   L  FA    IQLL+H+D  ++L    F+  LQE +P+I  +   P W+ RYS++ K+R +I+SKGYI
Sbjct:   84 DEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYI 266
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Match: sp|O94246|GSH0_SCHPO (Putative glutamate--cysteine ligase regulatory subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC737.06c PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.208e-13
Identity = 47/151 (31.13%), Postives = 76/151 (50.33%), Query Frame = 2 
Query:  323 CNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPS---WVCRYSIVYKNRSVIRSKGYI 766
             NE+   I  I P+ I S +      + W  LEE V + +I  LGV +F + +L+ +  SV + P   QI   + C +   L +FA  HH++L  H D   +L  +   + + +  P I     +P+   WV RY+I+ ++ +VI  KGYI
Sbjct:  140 SNEIYDSIDEIPPQEIQSWV------DTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINIGQNCKLPNDLLNFADRHHLKLFFHSDPSALLSESEITSVIHKACPEIP----NPARVDWVIRYTILTRHTAVIHQKGYI 280
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Match: A0A564Y432 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS2691 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 5.290e-33
Identity = 80/247 (32.39%), Postives = 136/247 (55.06%), Query Frame = 2 
Query:   98 DEILKCSIQVLKCYMKNENLFANNKLSDVEVECI---NHKDLILD-DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSI-----CMGDVY------------ELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIE----SVDPSWVCRYSIVYKNRSVIRSKGY 763
            DE+  C   V++C+           L D E  CI    H+D I D +RS + V+ KLFL  ++++ I + + +    L   ++++L++S++PE    +I     C+G  +            ++W  LEE+V+ ++I +LGV D  + ++E ++ +V+I P +NQ++    C +S+ L++FA  H IQLL+HHD  D+LP    + +LQ +M N   +       PSW+  Y+  YKNR V+RSKGY
Sbjct:   31 DEVETCIKTVMQCWHSCNK----TNLKDEEYLCIACDKHRDKIPDWERSSVTVSAKLFLFTDDHDAIRQAVNKTLDRLGLEDMETLVVSLSPELSRGAIVDHPPCLGTTWARIPQNTAHSLLKIWPVLEEFVETNKIFRLGVRDMPVSEMEALYTAVKIPPKVNQVFIGSTCLMSEELENFAKQHDIQLLTHHDSADMLP----EEKLQSVMRNEFCDEDGAGWRPSWLVSYAEYYKNRGVVRSKGY 269
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Match: A0A068WDE9 (Glutamate cysteine ligase regulatory OS=Echinococcus granulosus OX=6210 GN=EgrG_000887650 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.139e-31
Identity = 80/246 (32.52%), Postives = 134/246 (54.47%), Query Frame = 2 
Query:   98 DEILKCSIQVLKCY--MKNENLFANNKLSDVEVECINHKDLILD-DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSI-----CMG------------DVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIM----IESVDPSWVCRYSIVYKNRSVIRSKGY 763
            +E+  C   V+ C+   +N +L     L    + C  H+D I + +RS++ V+ KLFL  ++++ I + + +    L   +L++LIIS++PE    ++     CMG             + ++W  LEE+V+ ++I +LGV D  +  LE +  +V+I P +NQ++    C +   L++FA  H IQLL+HHD  D+LP    + +LQ +M N           PSW+ RY+  YKNR V+RS+GY
Sbjct:   31 EEVETCIRTVMHCWHSRRNADLCEQETLC---IACDKHRDKIPESERSNVIVSAKLFLFTDDHDSIRQAVNKTLDRLGLEDLETLIISLSPELSRGALVDHPPCMGLPWARIPQNTAHSLLKIWPVLEEFVEGNKIFRLGVSDMPVTDLEALCAAVKIPPKVNQVFIGSTCLLGDELENFAKLHSIQLLTHHDSADMLP----EEKLQAVMRNEFSAEDAAGWRPSWLMRYAEYYKNRGVVRSRGY 269
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Match: A0A068YG87 (Glutamate cysteine ligase regulatory subunit OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000887650 PE=4 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.131e-31
Identity = 80/246 (32.52%), Postives = 134/246 (54.47%), Query Frame = 2 
Query:   98 DEILKCSIQVLKCY--MKNENLFANNKLSDVEVECINHKDLILD-DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSI-----CMG------------DVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIE----SVDPSWVCRYSIVYKNRSVIRSKGY 763
            +E+  C   V+ C+   +N +L     L    + C  H+D I + +RS++ V+ KLFL  ++++ I + + +    L   +L++LIIS++PE    ++     CMG             + ++W  LEE+V+ ++I +LGV D  +  LE +  +V+I P +NQ++    C +   L++FA  H IQLL+HHD  D+LP    + +LQ +M N           PSW+ RY+  YKNR V+RS+GY
Sbjct:   31 EEVETCIRTVMHCWHSRRNADLCEQETLC---IACDKHRDKIPESERSNVIVSAKLFLFTDDHDSIRQAVNKTLDRLGLEDLETLIISLSPELSRGALVDHPPCMGLPWARIPQNTAHSLLKIWPVLEEFVEGNKIFRLGVSDMPVTDLEALCAAVKIPPKVNQVFIGSTCLLGDELENFAKLHSIQLLTHHDSADMLP----EEKLQAVMRNEFSAEDAVGWRPSWLMRYAEYYKNRGVVRSRGY 269
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Match: A0A0X3NHG5 (Glutamate--cysteine ligase regulatory subunit OS=Schistocephalus solidus OX=70667 GN=GSH0 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 1.791e-30
Identity = 80/241 (33.20%), Postives = 126/241 (52.28%), Query Frame = 2 
Query:   98 DEILKCSIQVLKCYMKNENLFANNKLSDVEVECINHKDLILD-DRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPET--------------ICSSICMGDVYEL---WSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSN-LFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGY 763
            DE  KC   VL C+ KN          ++ V C  H+D I D +R D+ V  K+FL   + + + + + +    L   ++++LI+S+ PE                 + IC G    L   W  LEE+VK +++ +LGVCD + +++E +  SV+I P +NQ++    C +S  L++FA  H +QL +H D  DIL ++ L     QE  P+       P W+ RYS ++K+R VIRSKGY
Sbjct:   31 DEPTKCIETVLHCWHKNTACKDLRTTEEINVACELHRDKIPDSERPDICVTAKVFLSTYDADAVRQAVNRTLDRLGLADIETLIVSVDPELSKAPITDVPPCMDFPSAKICKGTAESLLKVWPILEEFVKSNKVFRLGVCDMSADEMELLHSSVKIPPKVNQLFLGSSCLISDELQNFAKLHDVQLTTHQDSSDILRTDWLVSIISQEFSPDDAT-GWRPLWLVRYSEMFKHRGVIRSKGY 270
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Match: A0A158QEM4 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7636 PE=4 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.387e-29
Identity = 81/261 (31.03%), Postives = 139/261 (53.26%), Query Frame = 2 
Query:   98 DEILKCSIQVLKCYMKNENLFANNKLSDVEVECI---NHKDLILD-DRSDLRVNVKLFLINNNYEDISKH-----LAQIFKY---------LECNELDSLIISITPETICSSI-----CMGDVY------------ELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIE----SVDPSWVCRYSIVYKNRSVIRSKGY 763
            DE+  C   V++C+           L D E  CI    H+D I D +RS + V+ KLFL  ++++ I +      L ++ ++         L   ++++L++S++PE    +I     C+G  +            ++W  LEE+V+ ++I +LGV D  + ++E ++ +V+I P +NQ++    C +S+ L++FA  H IQLL+HHD  D+LP    + +LQ +M N   +       PSW+  Y+  YKNR V+RSKGY
Sbjct:   31 DEVETCIKTVMQCWHSCNK----TNLKDEEYLCIACDKHRDKIPDWERSSVTVSAKLFLFTDDHDAIRQAVNKSMLRKMLQFITLVSSLDRLGLEDMETLVVSLSPELSRGAIVDHPPCLGTTWARIPQNTAHSLLKIWPVLEEFVETNKIFRLGVRDMPVSEMEALYTAVKIPPKVNQVFIGSTCLMSEELENFAKQHDIQLLTHHDSADMLP----EEKLQSVMRNEFCDEDGAGWRPSWLVSYAEYYKNRGVVRSKGY 283
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Cavefish
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:103026947])

HSP 1 Score: 108.997 bits (271), Expect = 8.988e-27
Identity = 57/189 (30.16%), Postives = 102/189 (53.97%), Query Frame = 2 
Query:  200 NHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            N + +  ++R +L+V+VKLFL   +   I   +      L  ++LDS+II+  P     ++ +  +  LW +LE  V+  ++  +G  D     LE ++   ++KPS NQ+     C +   L  FA +  IQLL+H+D  +++ +  FK  +QE   ++ +      WV RYSI+ K+R +I++KGY+
Sbjct:   75 NTEAITPEEREELKVSVKLFLSEADCSAIGNAVDMACVSLGVSQLDSVIIAPPPLPEGETLALAHLQPLWEELEGLVRSQKVAAIGTSDLDKALLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKEFDIQLLTHNDSKEMISAEGFKEAVQESSKDLQVADWRLEWVLRYSIIVKSRGIIKAKGYL 263
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Sea Lamprey
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:ZFIN;Acc:ZDB-GENE-030131-5906])

HSP 1 Score: 112.464 bits (280), Expect = 1.117e-28
Identity = 62/231 (26.84%), Postives = 118/231 (51.08%), Query Frame = 2 
Query:   89 TSTDEILKCSIQVLKCYMKNENLFANNK-----LSDVEVECINHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            + ++E++ C    L  +    + FA ++       DV V C   + +  +DR DL++  K+FL+  + + I   + +    L  + LD+++++  P      +    +   W++LE  +  H++  +G  D   + LE+++   ++KP+ NQ+     C +   L  FA ++ IQLL+H+D  +ILP+  F+  +  + P    E   P WV RYS++ K R V++SKGY 
Sbjct:   42 SPSEELVDCVRSTLSDWASKLDGFARDQSPSCLAQDVCV-CPGTEKISAEDRDDLKITAKIFLLGGDAQVIKPAVEKACMALGVSLLDAVVLAPPPLPEEEKLTADRLSPYWAELESLIAAHKVATIGTSDLDSQLLEELYHWAQVKPASNQVNLASCCVMPPELTAFAKEYDIQLLTHNDPTEILPTKSFQDMVATVAPE-QAEVWTPLWVLRYSVIIKCRGVLKSKGYF 270
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Nematostella
Match: EDO44307 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWF3])

HSP 1 Score: 83.1889 bits (204), Expect = 3.993e-18
Identity = 49/181 (27.07%), Postives = 89/181 (49.17%), Query Frame = 2 
Query:  218 LDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKG 760
            LD+R  L+++ K+F I+ N   +   L +    L  + +++LI+++ P+          +   W ++E+      +  LGVCD     LE ++    IKP I+Q+     C + ++   ++    IQLL+H D  ++L     +  L  +   + +    PSWV RYS++ K R VI+SK 
Sbjct:   88 LDERMHLKISAKIFAISLNPAVVRDSLFRAMLELGVDSIETLILTL-PDIQDPEQHFTTIKSFWQEMEQLYDSGYVQTLGVCDLDKPTLEKLYDWARIKPEIDQVNLASCCVMPKASSTYSKTRDIQLLTHADPREMLSEERLQQVLGVVSEEMNVAGWRPSWVMRYSVLVKCRGVIKSKA 267
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Medaka
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:101162516])

HSP 1 Score: 100.523 bits (249), Expect = 1.150e-23
Identity = 54/182 (29.67%), Postives = 95/182 (52.20%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            ++R +L+V+VKLFL       I   +    + L  ++LDS+II+  P  + +   +  +   W +LE  V+  +I  +G  D   + LE ++   ++KPS NQ+     C +   L  FA D  IQLL+H D  +++ +  F   ++E   +  +      WV RYS++ K+R + +SKGY+
Sbjct:   83 EEREELKVSVKLFLCEAGQSAIGDAVDLACQTLAVSQLDSVIIA-PPPPLEADQSLAHLQPAWEQLEALVRSQKIAAIGTSDLDKDLLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKDFDIQLLTHSDPKELMSAESFLEAMREGARDQSLSGWRLEWVLRYSVIVKSRGIFKSKGYL 263
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Medaka
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:101162516])

HSP 1 Score: 100.138 bits (248), Expect = 1.400e-23
Identity = 54/182 (29.67%), Postives = 95/182 (52.20%), Query Frame = 2 
Query:  221 DDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYI 766
            ++R +L+V+VKLFL       I   +    + L  ++LDS+II+  P  + +   +  +   W +LE  V+  +I  +G  D   + LE ++   ++KPS NQ+     C +   L  FA D  IQLL+H D  +++ +  F   ++E   +  +      WV RYS++ K+R + +SKGY+
Sbjct:   84 EEREELKVSVKLFLCEAGQSAIGDAVDLACQTLAVSQLDSVIIA-PPPPLEADQSLAHLQPAWEQLEALVRSQKIAAIGTSDLDKDLLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKDFDIQLLTHSDPKELMSAESFLEAMREGARDQSLSGWRLEWVLRYSVIVKSRGIFKSKGYL 264
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Planmine SMEST
Match: SMESG000053228.1 (SMESG000053228.1)

HSP 1 Score: 490.73 bits (1262), Expect = 1.521e-175
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 2 
Query:   20 MNEMLIHTXXXXXXXXXXXXPQLTSTDEILKCSIQVLKCYMKNENLFANNKLSDVEVECINHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYISKVFWN 784
            MNEMLIHTSNIINWKSIKNNPQLTSTDEILKCSIQVLKCYMKNENLFANNKLSDVEVECINHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYISKVFWN
Sbjct:    1 MNEMLIHTSNIINWKSIKNNPQLTSTDEILKCSIQVLKCYMKNENLFANNKLSDVEVECINHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYISKVFWN 255
[Close]
BLAST of Aldo_ket_red domain-containing protein vs. Planmine SMEST
Match: SMESG000005952.1 (SMESG000005952.1)

HSP 1 Score: 100.908 bits (250), Expect = 3.529e-24
Identity = 71/230 (30.87%), Postives = 123/230 (53.48%), Query Frame = 2 
Query:   89 TSTDEILKCSIQVLKCYM-KNENLFANNKLSDVEVECINHKDL-ILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELD---SLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGY 763
            +++D+I++C   V+KC+   N+  F ++     +++C+ H+D   L +  D+R+ VK+F+ +   ED    L + F+Y+  N +D   + +I   P+     + +  +  LW+ L   +  H+I +LG+ D  L QL++  ++V + PSINQ      CSV   LKDFA  H I+LLSH+D   ++        L   +P        P WV + S +Y++RSVI+S  Y
Sbjct:   24 SNSDKIIECFRCVIKCWKDSNQESFEHS----TDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLED----LEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISLLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQSCSVACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEY 242
The following BLAST results are available for this feature:
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GCLM1.913e-2632.24glutamate-cysteine ligase modifier subunit [Source... [more]
GCLM3.473e-2632.24glutamate-cysteine ligase modifier subunit [Source... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
E01A2.15.190e-1827.14pep chromosome:WBcel235:I:4140649:4142367:1 gene:W... [more]
E01A2.18.230e-1827.14pep chromosome:WBcel235:I:4139514:4142335:1 gene:W... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Gclm1.011e-1723.00gene:FBgn0046114 transcript:FBtr0334605[more]
Gclm1.011e-1723.00gene:FBgn0046114 transcript:FBtr0084336[more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gclm1.566e-2330.69glutamate-cysteine ligase, modifier subunit [Sourc... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gclm2.475e-2628.96glutamate-cysteine ligase, modifier subunit [Sourc... [more]
gclm2.864e-1930.00glutamate-cysteine ligase, modifier subunit [Sourc... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Gclm3.454e-2631.69glutamate-cysteine ligase, modifier subunit [Sourc... [more]
Gclm3.454e-2631.69glutamate-cysteine ligase, modifier subunit [Sourc... [more]
Gclm1.367e-1227.89glutamate-cysteine ligase, modifier subunit [Sourc... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P48508|GSH0_RAT1.240e-2731.69Glutamate--cysteine ligase regulatory subunit OS=R... [more]
sp|Q2T9Y6|GSH0_BOVIN1.820e-2731.69Glutamate--cysteine ligase regulatory subunit OS=B... [more]
sp|P48507|GSH0_HUMAN1.668e-2532.24Glutamate--cysteine ligase regulatory subunit OS=H... [more]
sp|O09172|GSH0_MOUSE2.416e-2531.69Glutamate--cysteine ligase regulatory subunit OS=M... [more]
sp|O94246|GSH0_SCHPO4.208e-1331.13Putative glutamate--cysteine ligase regulatory sub... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A564Y4325.290e-3332.39Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A068WDE91.139e-3132.52Glutamate cysteine ligase regulatory OS=Echinococc... [more]
A0A068YG872.131e-3132.52Glutamate cysteine ligase regulatory subunit OS=Ec... [more]
A0A0X3NHG51.791e-3033.20Glutamate--cysteine ligase regulatory subunit OS=S... [more]
A0A158QEM41.387e-2931.03Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gclm8.988e-2730.16glutamate-cysteine ligase modifier subunit [Source... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gclm1.117e-2826.84glutamate-cysteine ligase, modifier subunit [Sourc... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO443073.993e-1827.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gclm1.150e-2329.67glutamate-cysteine ligase modifier subunit [Source... [more]
gclm1.400e-2329.67glutamate-cysteine ligase modifier subunit [Source... [more]
back to top
BLAST of Aldo_ket_red domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000053228.11.521e-175100.00SMESG000053228.1[more]
SMESG000005952.13.529e-2430.87SMESG000005952.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008411 ID=SMED30008411|Name=Aldo_ket_red domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1272bp
AACTGCGTAATTTAAGAAAATGAATGAAATGCTAATCCACACAAGCAACA
TTATTAATTGGAAAAGCATAAAAAACAATCCACAATTAACATCCACAGAT
GAAATATTAAAATGCAGTATTCAAGTTTTGAAATGTTATATGAAAAATGA
GAATTTATTTGCCAACAATAAGCTTTCAGATGTTGAAGTGGAGTGTATTA
ATCACAAAGATTTAATTTTAGATGATAGATCAGATTTAAGAGTAAATGTG
AAACTATTTCTTATTAACAACAATTACGAAGACATTTCAAAACATTTAGC
ACAAATATTTAAATATTTGGAATGCAATGAATTGGATTCGTTGATTATTT
CCATCACTCCCGAAACTATATGCTCTTCAATCTGTATGGGTGATGTGTAT
GAACTTTGGTCGAAATTAGAAGAATATGTTAAAGATCATAGGATTTTACA
GCTGGGAGTTTGTGATTTCACATTAGAACAGTTAGAGGATATGTGGCAAA
GCGTCGAGATAAAACCGTCGATTAATCAAATTTATTGGAAACGTATGTGT
AGTGTGTCTCAATCACTTAAAGATTTTGCAGCTGATCATCACATTCAGTT
GCTTTCGCATCACGACAAACCAGATATCTTGCCAAGTAATCTATTCAAAA
CCAGGCTCCAAGAAATAATGCCAAATATAATGATTGAATCAGTAGACCCC
TCGTGGGTTTGCCGGTATTCTATTGTGTATAAAAATAGGAGTGTCATCAG
ATCTAAAGGCTATATTTCAAAGGTTTTCTGGAATTGAAATTTCCACTTGA
TTTTGATTTCATTCTCATTACGCAGTTTTATTTTTCGTAAGTATCGATTA
ATATTTTAGTTCGTGTTGTTGGTTGAAAGTTTTCCTATCGAAAAGGATTT
AATGAAATGAAATAATTTACTTGTGTTGCTTGATTTTTTCTTTGTTGATA
TTTATTGATTGATATTGGTTGATTGTTATTTGATGAAAGTAAAATAAAAT
TTCATTTATCTAAAAGTCAATCATGCAAATCTTCGGTACATTAAATCAAT
GAATGTATCAATGTTTATGATCCTGCGCAAGGCGTCGTGCCGTCGCATCT
GCAACACTACAAAAACTTACAATAGCAAATAAATACTGTGAATTGAGAGA
TTAAAACTCCTTTACGTTTAGAGATTCTAGGTGTGAATCGATTTAGTGCC
GTTATCATCGTAAACAATTCAAATTTTGAATTTATTGTGATTTTTAATGT
AAATACCAAGTAATGTGTTTAG
back to top

protein sequence of SMED30008411-orf-1

>SMED30008411-orf-1 ID=SMED30008411-orf-1|Name=SMED30008411-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=256bp
MNEMLIHTSNIINWKSIKNNPQLTSTDEILKCSIQVLKCYMKNENLFANN
KLSDVEVECINHKDLILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYL
ECNELDSLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDF
TLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDK
PDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGYIS
KVFWN*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036812NADP_OxRdtase_dom_sf
IPR032963Gclm
IPR023210NADP_OxRdtase_dom
Vocabulary: biological process
TermDefinition
GO:0006750glutathione biosynthetic process
Vocabulary: molecular function
TermDefinition
GO:0016874ligase activity
GO:0035226glutamate-cysteine ligase catalytic subunit binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3DG3DSA:3.20.20.100coord: 47..216
e-value: 1.2E-15
score: 59.0
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILYSSF51430NAD(P)-linked oxidoreductasecoord: 69..196
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 69..194
e-value: 2.0E-8
score: 33.8
IPR032963Glutamate--cysteine ligase regulatory subunitPANTHERPTHR13295GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNITcoord: 3..249