G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30008402
Length (bp)1084
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008402) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008402) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008402) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30008402) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008402

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior region of the whole animalSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
body wall musculatureSMED30008402h1SMcG0014792 dd_Smed_v4_12494_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: chrm4b (cholinergic receptor, muscarinic 4b [Source:ZFIN;Acc:ZDB-GENE-090410-7])

HSP 1 Score: 49.2914 bits (116), Expect = 4.247e-6
Identity = 39/114 (34.21%), Postives = 53/114 (46.49%), Query Frame = 2 
Query:  707 EAQTFGKNNVSKS--RITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQA---WTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKIFFSRFSCKF*NLS 1033
            + Q    N VSK+  R   R+ KVTKT+L V +AF I   P+ V+  + T+          W      W V Y L YVN+ VN   YA  +  F    K++      CKF NL+
Sbjct:  348 QVQRQTSNMVSKNAKRKALRERKVTKTVLAVLLAFIITWTPYSVMVLIGTFC--------RWCVPEVLWMVGYFLCYVNSTVNPVCYALCNATFKRTFKRL----LRCKFKNLA 449
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A2H1CRL0 (7 transmembrane receptor OS=Fasciola hepatica OX=6192 GN=D915_03041 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 1.190e-35
Identity = 117/346 (33.82%), Postives = 176/346 (50.87%), Query Frame = 2 
Query:   53 DDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHR--FPSTYRLQVIGLYLTFFICCSLN-IHLLFDTELEFDMKTNSTG---------CTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITS--------RLPDSHTTPSS----SVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKIF-FSRFSC 1015
            D  +W+  + + I +G+ EN +SV VL R++F  +PSR++L+ L+I D ++L++GLGRYWL      Y        C+++S  V+  SH+SS +VAL+ +ER  AV   H+   PS  +L   G  + F  C +++ +HL +        KT +           CT+ S  VA   D  D + L  +P ++II CN+  I+ L+  S+++          R    H   +     S+ +  +  T  K N  K     R+ ++T TLL VT  F + N PF +V+ LRT  SE  T    W  AW  TY LVY+NNC+N PIY   H  F  ELK +    R SC
Sbjct:   39 DSPVWMYVAPIFIIIGLAENCLSVAVLSRERFTHVPSRVTLVVLAIVDCVVLLIGLGRYWLSVTFQVYPVEWSNAWCKIISTFVLFSSHLSSCLVALLSVERFFAVKLPHKQFRPSIDKL---GTVILFLFCLAVDTVHLKYIVLQSGLNKTETLENVEILHVYVCTSESEWVAFWQDIADILLLFGMPFLLIIICNTYTIVSLRRRSNQLDRMTKCRDKVRSLGEHGVKARRRWISLLVTEKLSTREKYNTIKPNYKDRNARITFTLLCVTFVFLLCNFPFALVHILRTANSERRT--EGWNIAWLFTYFLVYLNNCLNFPIYVVSHAQFRGELKTLLRCQRRSC 379
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183A5J7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2232 PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.681e-32
Identity = 111/316 (35.13%), Postives = 172/316 (54.43%), Query Frame = 2 
Query:   62 IWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRF--PSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITSRLPDSHTTPS-SSVRLKNEAQTFGKNNVSKSRITK--RDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKI 994
            IW+  + +LI +GV EN +S++VL R +F  +PSR++L+ L+I D ++L +GL RYWL      +  + + W     S   +  SH+SS +VAL+ +ER  AV    +   PS  +L  + L           I +L D+  + + K     C++ S  VA+  D  D + L  LP I+I +CN  II+ ++  S ++        T P+ SS  +++E   FG+ + ++ R  +  R+ ++T TLL VT AF + N+PF VV+ +RT  S   T    W  AW VTY+LVYVNNC+N PIY      F  ELK +
Sbjct:   37 IWMYVAPILIIVGVTENCLSILVLTRDRFIHVPSRVTLVALAIVDCVVLFIGLIRYWLNVT---FDMHPVEWN----SAWFLFSSHLSSCLVALLSVERFLAVCLPRKIFRPSIDKLGTVLL-----------IVVLTDSNDKDEHK--KYVCSSESDWVALWQDIADVLLLFVLPCIVITTCNIYIILAIRRNSKRVGRSGKRRDTLPTISSKPVESEQTAFGRKSPNRIRGGEKHRNVRITTTLLCVTFAFLLCNLPFAVVHIMRTADSSRRT--REWNIAWLVTYILVYVNNCLNFPIYVVSQEQFRNELKNL 330
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A5J4NX88 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0002405 PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.690e-31
Identity = 102/355 (28.73%), Postives = 170/355 (47.89%), Query Frame = 2 
Query:   50 MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHR-FPSTYRLQ---VIGLYL--TFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAXXXXX----------------XXXXXXXXXXXXXXXXXXXVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNV----------SKSRITKRDN--------KVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKI 994
            +D  +W     +LIP+G+ EN++++ VL R++F  +PSR +LI ++I D ++L++GL R+WL      Y  +     C+ LS  VI +SH+SS +VAL+ +ER  AV    R F     +    V+G+    TFFI    +  +L D     D +     CTN     A  +D LD++ L                  +        N            ++T  +  +    ++   +K  +  FG+ +           +K +  K+++        ++T TLL+VT  F +  +PF ++N LRT  SE    S +W  +W V+Y+LVY NNC+N PIY   +P+F  E+K +
Sbjct:   19 LDALLWHYIPPILIPIGLTENSLALFVLSRRRFVRLPSRATLISIAIVDSVVLVLGLIRFWLNVTFNVYLPSLSEMCCKTLSATVIILSHLSSFLVALLSVERFFAVFMPQRQFKPPIDMMGIPVLGVLCAGTFFI--YFHYTVLIDVPNASDHQHQKV-CTNMDDTTAFWLDILDSLMLSVIPFIIILLCNGYIIWRMKRNSRRLGNFARGSPSPETKFQLTQTIEGASLPTNTCTNVKRISVEFGQADQCRHQSIITFGTKQKPKKKEHPISSTRRTRITNTLLWVTFTFLVCTLPFAIINLLRTADSERR--SIAWNTSWLVSYVLVYTNNCLNFPIYIISNPLFRSEVKDL 368
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: G7Y6K2 (G-protein coupled receptor OS=Clonorchis sinensis OX=79923 GN=CLF_101785 PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.237e-30
Identity = 116/338 (34.32%), Postives = 176/338 (52.07%), Query Frame = 2 
Query:   53 DDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSH-RFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMK--TNSTGCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKI---------TSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITK---------RDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKIFFS 1003
            D ++WL    +LIP+G  EN ++V+VL R +F  +P R++LI ++I D+I+L  GL R+W   +           +C++LS LVI+ SH+SS +VAL+ +ER  +V   H RF +   +   G+ +  F C  +       T L   +    +   C ++S  VAII+D LD   LC +PL+II   N+ II +L   + K+         TSR    H T  S +   ++     +    K R+T          R  ++T TLL +T +F +F  PF VV+  RT  +E  T  + W  +W +TY+LVYVNNC+N PIY    P F  ELK +  S
Sbjct:   19 DASVWLYIPPILIPVGFCENILAVLVLARPRFSQLPCRITLIAIAIVDMIVLFFGLTRFWTNVMFDTNLQTLSEAVCKMLSPLVISCSHLSSLLVALLSVERFCSVYRPHNRFHTP--VDYFGVPVLTFFCLGIFFVYYHYTILTPGIPGTKHDKVCVSTSQTVAIILDLLDTALLCLIPLVIISVSNTFIIYRLYQNTKKLDNYHNSVQSTSR----HMTGDSEISSVDQRSCLPQLIRKKPRVTHPSPKLVGFNRQKRITITLLLITSSFVLFTFPFTVVHLSRTVDNERRT--SPWNMSWLITYILVYVNNCLNFPIYIISQPYFYGELKAMMRS 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4V3SEV5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005529 PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.860e-30
Identity = 116/334 (34.73%), Postives = 179/334 (53.59%), Query Frame = 2 
Query:   53 DDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSH-RFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMK--TNSTGCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITS-----RLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITK---------RDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKIFFS 1003
            D ++WL    +LIP+G  EN ++V+VL R +F  +P R++LI ++I D+I+L  GL R+W   +           +C++LS LVI+ SH+SS +VAL+ +ER  +V   H RF +   +  +G+ +  F C  +       T L   +    +   C ++S  VAII+D LD   LC LPL++I   N+ II +L   + K+ S     +    H T  S +  +++     +    K R+T+         R  ++T TLL +T +F +F  PF VV+  RT  +E  T  +SW  +W VTY+LVYVNNC+N PIY    P F  ELK +  S
Sbjct:   19 DASVWLYIPPILIPVGFCENILAVLVLARPRFSQLPCRITLIAIAIVDMIVLFFGLTRFWTNVLFDTNLQTLSEAVCKMLSPLVISCSHLSSLLVALLSVERFCSVYRPHNRFHTP--VDYVGVPVLTFFCFGIFFVYYHYTILTPGIPGTKHDKVCVSTSQTVAIILDLLDTALLCLLPLVVISVNNTFIIYRLYQNTKKLDSYHNTVQFTSRHMTGGSEISSEDQRSCLPQLIRKKPRVTQPSPKMVAFNRQKRITITLLLITSSFVLFTFPFTVVHLSRTVDNERRT--SSWNMSWLVTYILVYVNNCLNFPIYIISQPYFYGELKAMMRS 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000075377.1 (SMESG000075377.1)

HSP 1 Score: 520.776 bits (1340), Expect = 0.000e+0
Identity = 292/309 (94.50%), Postives = 295/309 (95.47%), Query Frame = 2 
Query:   50 MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVT---YLLVYVNNCVNLPIYAFIHP 967
            MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAIFLCFLPLIIIISCNSCIIIKLKIVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVT   + L+       LP   F+HP
Sbjct:    1 MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAIFLCFLPLIIIISCNSCIIIKLKIVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTRQKHQLLTTYKQFVLPA-GFVHP 308
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000075377.1 (SMESG000075377.1)

HSP 1 Score: 520.005 bits (1338), Expect = 0.000e+0
Identity = 292/309 (94.50%), Postives = 295/309 (95.47%), Query Frame = 2 
Query:   50 MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVT---YLLVYVNNCVNLPIYAFIHP 967
            MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAIFLCFLPLIIIISCNSCIIIKLKIVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVT   + L+       LP   F+HP
Sbjct:    1 MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNSTGCTNSSWKVAIIVDSLDAIFLCFLPLIIIISCNSCIIIKLKIVSHKITSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTRQKHQLLTTYKQFVLPA-GFVHP 308
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000062905.1 (SMESG000062905.1)

HSP 1 Score: 218.779 bits (556), Expect = 2.688e-68
Identity = 147/340 (43.24%), Postives = 205/340 (60.29%), Query Frame = 2 
Query:   65 WLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKTNST--GCTNSSWKVAIIVDSLDAXXXXXXXXXXXXXXXXXXXXXXXXVSHKITSRLPDSHTTPSSSVRLK---------NEAQT--FGKNNVSKS---------RITKRDNKVTKTLLFVTMAFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPIYAFIHPIFMEELKKIFFSRFSCK 1018
            WLI SS+ IP GV EN +SV+VL+R +F  MPSR +LI LSIND ++L  G+ RYWL AV      N   W C++LSYLVI +SHISS +VAL+C+ER  A+V+ +    +  +Q+ GLY+ +F    +N+H++F+T L+ + +TNST   CT+ +W  A+ VD +D+I LCF PL +I+  N CII+KL+ VS+++      +  + SS ++ K         N +Q      N VSK          +  KRD +VT  LLFV+  F   N PFGVV+ LRTY + +NTP+  W  AW  TYLLVY NNC+N PIY     +F   +     S  SCK
Sbjct:   14 WLIASSIFIPFGVSENILSVLVLIRPRFSDMPSRYTLIALSINDGLVLFFGMFRYWLVAVFEYNPMNYSLWSCKVLSYLVITLSHISSGLVALVCVERFFAIVYYYILLPSKTIQIRGLYIVYFSSLFVNLHVIFNTILK-ENETNSTDFSCTSLNWNTAMAVDIIDSILLCFCPLFVILFSNGCIIVKLRKVSNQMQQNTRPNKVSMSSPLKQKRLLSITMNDNSSQITCHCSNVVSKKYQSYHRKRWKPWKRDTQVTIMLLFVSFVFLFCNFPFGVVHLLRTYVNGENTPTRPWDMAWISTYLLVYFNNCINFPIYIISQSVFRNHV----ISLLSCK 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042142.1 (SMESG000042142.1)

HSP 1 Score: 51.9878 bits (123), Expect = 4.201e-7
Identity = 29/100 (29.00%), Postives = 54/100 (54.00%), Query Frame = 2 
Query:   62 IWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDLILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALICIERL 361
            +W++    ++ +G I N +S VVL R+    + S + L  L+I D ++L+ GL R W++ VT     N     C+L   + ++ S IS  ++  + +ER+
Sbjct:   70 LWMLIPIFIVAMGSIGNFLSAVVLCRKSMSHISSYIYLATLAIFDELILLCGLFRKWIEHVTDWSLENHSISFCKLFQVIGVSSSFISVWLIVTLTVERV 169
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
chrm4b4.247e-634.21cholinergic receptor, muscarinic 4b [Source:ZFIN;A... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1CRL01.190e-3533.827 transmembrane receptor OS=Fasciola hepatica OX=6... [more]
A0A183A5J72.681e-3235.13G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A5J4NX883.690e-3128.73G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
G7Y6K21.237e-3034.32G-protein coupled receptor OS=Clonorchis sinensis ... [more]
A0A4V3SEV52.860e-3034.73G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000075377.10.000e+094.50SMESG000075377.1[more]
SMESG000075377.10.000e+094.50SMESG000075377.1[more]
SMESG000062905.12.688e-6843.24SMESG000062905.1[more]
SMESG000042142.14.201e-729.00SMESG000042142.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008402 ID=SMED30008402|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1084bp
GTGTATTCCTATTATTTAAAGTTAAACTCTATTTCTATTTTTCCTAAATA
TGGATGATACAATTTGGTTGATTTGCTCCTCAGTCCTCATTCCTTTAGGC
GTTATTGAAAATGCAATTTCTGTCGTCGTTCTGGTCCGTCAGAAATTCCA
AGCAATGCCATCTCGACTCAGTCTCATATGTCTGTCAATTAATGACTTGA
TCCTGTTGATTGTCGGACTGGGAAGGTATTGGTTGAAGGCCGTAACCGGA
AGGTACGCTGCGAATGACATTTACTGGCTTTGTCGACTGTTATCTTATCT
TGTGATTGCAATGAGCCATATCAGCTCAACAATTGTTGCATTGATATGCA
TCGAGAGATTGGCCGCTGTTGTTTTTAGTCACAGATTCCCATCAACCTAC
AGGTTACAAGTCATTGGCCTCTATCTAACCTTCTTTATATGTTGTTCATT
GAATATTCATTTATTATTTGACACAGAATTGGAATTTGACATGAAGACTA
ACAGTACCGGATGTACTAATTCAAGTTGGAAGGTAGCAATAATTGTCGAC
AGTCTTGACGCAATTTTTCTTTGTTTTTTACCTCTGATAATTATTATCTC
TTGCAATTCGTGCATCATTATTAAATTGAAAATTGTGTCCCACAAAATCA
CCTCAAGACTCCCAGATTCTCACACAACTCCGAGTTCGTCGGTAAGACTG
AAAAACGAGGCTCAAACCTTTGGGAAGAATAATGTTTCAAAATCAAGAAT
AACCAAACGTGACAATAAAGTGACAAAGACTTTGCTCTTCGTGACAATGG
CGTTCTTCATTTTCAATGTTCCGTTTGGTGTAGTAAATTTTCTTCGAACT
TACGCCAGTGAAGACAATACCCCTTCCAATAGTTGGACCCAGGCATGGAC
CGTGACGTATTTATTGGTTTACGTTAACAACTGCGTCAACTTACCGATCT
ACGCATTCATTCACCCTATCTTCATGGAAGAATTGAAGAAAATATTTTTC
AGTAGATTTTCATGTAAATTTTGAAATCTGTCACGGAGAATCGGCAACAA
AACATGCAGTATGGAAAAGGTTAATTGATGGTGT
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protein sequence of SMED30008402-orf-1

>SMED30008402-orf-1 ID=SMED30008402-orf-1|Name=SMED30008402-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=325bp
MDDTIWLICSSVLIPLGVIENAISVVVLVRQKFQAMPSRLSLICLSINDL
ILLIVGLGRYWLKAVTGRYAANDIYWLCRLLSYLVIAMSHISSTIVALIC
IERLAAVVFSHRFPSTYRLQVIGLYLTFFICCSLNIHLLFDTELEFDMKT
NSTGCTNSSWKVAIIVDSLDAIFLCFLPLIIIISCNSCIIIKLKIVSHKI
TSRLPDSHTTPSSSVRLKNEAQTFGKNNVSKSRITKRDNKVTKTLLFVTM
AFFIFNVPFGVVNFLRTYASEDNTPSNSWTQAWTVTYLLVYVNNCVNLPI
YAFIHPIFMEELKKIFFSRFSCKF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000240body wall musculature
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0004871signal transducer activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 283..309
score: 27.13
coord: 38..59
score: 19.9
coord: 85..107
score: 19.05
coord: 5..29
score: 18.14
coord: 241..265
score: 25.51
coord: 167..190
score: 21.11
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 21..297
e-value: 2.8E-20
score: 72.8
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 1..323
e-value: 1.4E-43
score: 151.1
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 4..320
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 4..320
NoneNo IPR availableCDDcd006377tm_classA_rhodopsin-likecoord: 6..303
e-value: 7.9446E-17
score: 76.9326
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 5..322
NoneNo IPR availableTMHMMTMhelixcoord: 5..27
NoneNo IPR availableTMHMMTMhelixcoord: 121..140
NoneNo IPR availableTMHMMTMhelixcoord: 40..62
NoneNo IPR availableTMHMMTMhelixcoord: 282..304
NoneNo IPR availableTMHMMTMhelixcoord: 82..101
NoneNo IPR availableTMHMMTMhelixcoord: 245..267
NoneNo IPR availableTMHMMTMhelixcoord: 169..191
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 20..301
score: 25.367