Histone-lysine N-methyltransferase SETMAR-like protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008358 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Human
Match: SETMAR (SET domain and mariner transposase fusion gene [Source:HGNC Symbol;Acc:HGNC:10762]) HSP 1 Score: 64.3142 bits (155), Expect = 1.635e-13 Identity = 31/71 (43.66%), Postives = 44/71 (61.97%), Query Frame = 2 Query: 203 GVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 G +LLHDNA H+ T + L EVLPHPPYSPDL+P+++H+ L+N L G F++ + + EF Sbjct: 582 GPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEF 652 HSP 2 Score: 29.6462 bits (65), Expect = 1.635e-13 Identity = 11/38 (28.95%), Postives = 21/38 (55.26%), Query Frame = 3 Query: 42 LHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQM 155 LHP ++ +W ++ Y L+ +T+ SE Y Q++ Sbjct: 526 LHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEI 563
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Human
Match: SETMAR (SET domain and mariner transposase fusion gene [Source:HGNC Symbol;Acc:HGNC:10762]) HSP 1 Score: 61.2326 bits (147), Expect = 1.452e-12 Identity = 31/71 (43.66%), Postives = 44/71 (61.97%), Query Frame = 2 Query: 203 GVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 G +LLHDNA H+ T + L EVLPHPPYSPDL+P+++H+ L+N L G F++ + + EF Sbjct: 443 GPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEF 513 HSP 2 Score: 29.6462 bits (65), Expect = 1.452e-12 Identity = 11/38 (28.95%), Postives = 21/38 (55.26%), Query Frame = 3 Query: 42 LHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQM 155 LHP ++ +W ++ Y L+ +T+ SE Y Q++ Sbjct: 387 LHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEI 424
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. UniProt/SwissProt
Match: sp|Q7JQ07|MOS1T_DROMA (Mariner Mos1 transposase OS=Drosophila mauritiana OX=7226 GN=mariner\T PE=1 SV=1) HSP 1 Score: 67.0106 bits (162), Expect = 4.472e-16 Identity = 38/97 (39.18%), Postives = 50/97 (51.55%), Query Frame = 2 Query: 164 NNTIQQK*TD---RLYGVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEFCKLFQALFIW 445 N +Q+K + R + V+ LHDNA H + + TL EVLPH YSPDL PSD+HL S+ +AL F+ WLDE+ F W Sbjct: 227 NRALQRKRPEYQKRQHRVIFLHDNAPSHTARAVRDTLETLNWEVLPHAAYSPDLAPSDYHLFASMGHALAEQRFDSYESVKKWLDEWFAAKDDEFYW 323 HSP 2 Score: 37.7354 bits (86), Expect = 4.472e-16 Identity = 16/32 (50.00%), Postives = 22/32 (68.75%), Query Frame = 3 Query: 60 ILCVWRDWKWIVLYELLDRNQTVNSEVYIQQM 155 +LCVW D ++ YELL R +TVN+ Y QQ+ Sbjct: 192 MLCVWWDQSGVIYYELLKRGETVNTARYQQQL 223
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. UniProt/SwissProt
Match: sp|Q53H47|SETMR_HUMAN (Histone-lysine N-methyltransferase SETMAR OS=Homo sapiens OX=9606 GN=SETMAR PE=1 SV=2) HSP 1 Score: 64.3142 bits (155), Expect = 6.517e-13 Identity = 31/71 (43.66%), Postives = 44/71 (61.97%), Query Frame = 2 Query: 203 GVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 G +LLHDNA H+ T + L EVLPHPPYSPDL+P+++H+ L+N L G F++ + + EF Sbjct: 582 GPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEF 652 HSP 2 Score: 29.6462 bits (65), Expect = 6.517e-13 Identity = 11/38 (28.95%), Postives = 21/38 (55.26%), Query Frame = 3 Query: 42 LHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQM 155 LHP ++ +W ++ Y L+ +T+ SE Y Q++ Sbjct: 526 LHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEI 563
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Match: A0A1I7WUB6 (Uncharacterized protein OS=Heterorhabditis bacteriophora OX=37862 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 4.100e-27 Identity = 46/76 (60.53%), Postives = 54/76 (71.05%), Query Frame = 2 Query: 164 NNTIQQK*TDRLYGVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVE 391 N I++K +R +GVLLL+DNA HI N+TK VI T EVLPHPPYS L P+DFHL SLSN + GV FN DVE Sbjct: 149 NTAIKEKGPNRQHGVLLLNDNARPHIANMTKEVIQTHGWEVLPHPPYSLALSPTDFHLFRSLSNDMRGVSFNTDVE 224 HSP 2 Score: 63.5438 bits (153), Expect = 4.100e-27 Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 3 Query: 3 SLCEQAHCEQKKDLHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQMHRPITLFNKNEP 191 S +QA K+DLHP + C+W DW+ ++ YELL+RNQTVN+E+Y+QQM R T + P Sbjct: 95 SSGKQAIPRVKQDLHPRKTMSCIWWDWEGLIHYELLERNQTVNAELYVQQMERLNTAIKEKGP 157
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Match: A0A1I7XA86 (Uncharacterized protein OS=Heterorhabditis bacteriophora OX=37862 PE=4 SV=1) HSP 1 Score: 100.908 bits (250), Expect = 3.788e-24 Identity = 54/99 (54.55%), Postives = 67/99 (67.68%), Query Frame = 2 Query: 164 NNTIQQK*TDRLYGVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEFCKLFQALFIW*TIQS 460 N I++K +R +GVLLL+DNA HI N+TK VI T EVLPHPPYS L P+DFHL SLSN + GV FN DVE +WLDEF + Q F +I++ Sbjct: 5 NTAIKEKGPNRQHGVLLLNDNARPHIANMTKEVIQTHGWEVLPHPPYSLALSPTDFHLFRSLSNDMRGVSFNTDVELRAWLDEFFESKQGNFCQRSIEN 103
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Match: A0A1I7XTN1 (Uncharacterized protein OS=Heterorhabditis bacteriophora OX=37862 PE=4 SV=1) HSP 1 Score: 94.7449 bits (234), Expect = 1.206e-20 Identity = 49/84 (58.33%), Postives = 58/84 (69.05%), Query Frame = 2 Query: 164 NNTIQQK*TDRLYGVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 N I++K +R +GVLLL+DNA HI N+TK I T EVLPHPPYS L P+DFHL SLSN + GV FN D E +WLDEF Sbjct: 109 NTAIKEKGPNRQHGVLLLNDNARPHIANMTKEAIQTHGWEVLPHPPYSLALSPTDFHLFRSLSNDMRGVSFNTDAELRAWLDEF 192
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Match: A0A158NRL0 (HTH_48 domain-containing protein OS=Atta cephalotes OX=12957 GN=105623384 PE=4 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 1.770e-19 Identity = 36/71 (50.70%), Postives = 49/71 (69.01%), Query Frame = 2 Query: 203 GVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 GV+ DNA H++ +T+ +L L +VLPHPPYSPDL PSDFHL SL N+L+G FN +E + L++F Sbjct: 230 GVVFHQDNARPHVSLITRQKLLELGWDVLPHPPYSPDLAPSDFHLFRSLQNSLNGKSFNSLIEIKNHLEKF 300 HSP 2 Score: 51.2174 bits (121), Expect = 1.770e-19 Identity = 25/53 (47.17%), Postives = 31/53 (58.49%), Query Frame = 3 Query: 33 KKDLHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQMHRPITLFNKNEP 191 K DLHP +LCVW DWK I+ YELL N+ +NSE Y Q+ T + P Sbjct: 171 KTDLHPKKVMLCVWWDWKGILYYELLPNNEMINSEKYCSQLDELKTAIEQKRP 223
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Match: Q8I8E8 (Camar1 transposase OS=Chymomyza amoena OX=7293 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 8.856e-19 Identity = 34/71 (47.89%), Postives = 44/71 (61.97%), Query Frame = 2 Query: 203 GVLLLHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLDEF 415 GV+ DNA H + +T+ + L EVL HPPYSPDL PSD+HL SL N+L+G F +D S L +F Sbjct: 239 GVIFHQDNARPHTSLMTRQKLRELGWEVLMHPPYSPDLAPSDYHLFRSLQNSLNGKTFGNDEAIKSHLVQF 309 HSP 2 Score: 56.225 bits (134), Expect = 8.856e-19 Identity = 27/61 (44.26%), Postives = 36/61 (59.02%), Query Frame = 3 Query: 9 CEQAHCEQKKDLHPLNRILCVWRDWKWIVLYELLDRNQTVNSEVYIQQMHRPITLFNKNEP 191 E A K D+H +L VW DWK +V +ELL RNQT+NS+VY QQ+ + N+ P Sbjct: 172 GEPAQTTSKADIHQKKVMLSVWWDWKGVVYFELLPRNQTINSDVYCQQLDKLNAAINEKRP 232
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Planmine SMEST
Match: SMESG000049199.1 (SMESG000049199.1) HSP 1 Score: 44.2838 bits (103), Expect = 7.264e-6 Identity = 26/65 (40.00%), Postives = 34/65 (52.31%), Query Frame = 2 Query: 215 LHDNAHHHITNLTKTVILTLR*EVLPHPPYSPDLVPSDFHLVWSLSNALHGVLFNDDVEF*SWLD 409 L NA H + ++ + VL HPPYSPDL SDF+L L +++ G FN D E LD Sbjct: 44 LQYNARPHTAAKSVQKLIDIGFTVLLHPPYSPDLATSDFYLFSPLKSSIRGRDFNGDNEIQVVLD 108 The following BLAST results are available for this feature:
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Histone-lysine N-methyltransferase SETMAR-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008358 ID=SMED30008358|Name=Histone-lysine N-methyltransferase SETMAR-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=551bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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