SCO-spondin
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008350 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 25
Homology
BLAST of SCO-spondin vs. Ensembl Human
Match: MUC5AC (mucin 5AC, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7515]) HSP 1 Score: 386.726 bits (992), Expect = 3.548e-108 Identity = 344/1299 (26.48%), Postives = 543/1299 (41.80%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMY--VLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV--EYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHT------TKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT-GSCDNTPQSIVDP-------PAGDNDICSTI--LNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD--------SVSGC-NSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTP-GDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDI--VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-----EGG--KHYDACASPCPQTCDTIGSNAPSHC-STTHCVEGC--SCPNGMIDLNG---ECV-----PPTTCPCFYNGQSYVNGAIIKME-NCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDD 4463 N C ++G +Y+TFD ++ + G C+Y+ + + ++I + ++ +V++ G V+ + G+ ++ + + L P S S ++I + Y V + +L+ ++ L+ D GLCG + + FGN K D P + C ++ F GC LV+ +Y C Q++C + +C L+ YS C+ G Q WR C +CP N + +C S C TC N Q S C++ V C C G V + G T C+ C C+Y+G + PG DC CTC+ W C E CP TC ++G F TFDG ++ + C YV +P +SA+ ++ + S+ LK+ + L +QT V I T+LP + + I I + + L + ++++++ L+ GLCG F+ +DF + + ++ T +C N S DP CS + +GP F C + K +Y+ + + + C+A+ + AC G+Q + + + C T + TCQ TCR L+E + C P GC CP + TG+CV S C C++ + IP G ++ + C C +G+L+C PVC V+ D + +GC SC D + C CP+ V+ +G C+ DCPC++ Y I + C C +RMW + C A C YGD HY T DGQ +SF G C YTL+ G + + N+ CG + TC+K ++I +GG LS G + I ++ I Q+ + + +D IGL +LWD+ T +++ L P F+G V GLCGN+D N++ + D ++F S+ + CP ++P +D C ANP RKSWA++ C ++ FA+C H + RYY C+ DAC C+ GGDCEC CT++A +A C +G+ V WRT ++CP+ C EG HY C PC +TC N C +EGC CP + +CV PP C +G+SY GA++ + NC+ C C + CT C+ T +F D Sbjct: 76 HNGRVCSTWGSFHYKTFDGDVFRFPGLCNYVFSEHCGAAYEDFNIQLRRSQESAAPTLSRVLMKVDGVVIQLTKGSVLV---NGHPVLLPFS----QSGVLIQQSSSYTKVEARLGLVLMWNHDDSLLLELDTKYA-----------NKTCGLCGDFNGMPVVSELLSHNTKLTPMEFGNLQKMDDPTDQCQDPVPEPPRNCSTGFGICEELLHGQLFSGCVALVDVGSYLEACRQDLCFCEDTD--------LLSCVCHTLAEYSRQCTHAGGLPQDWRGPDFCPQKCPNNMQYHECRSPCADTCSN--QEHSRACEDHCVAGCFCPEGTVLDDIGQTGCVPVSKCACVYNGAAYAPGATYSTDCTNCTCSGGRWSCQEVPCPGTCSVLGGAHFSTFDGKQYTVHG-DCSYVLTKPC-------------------------DSSAFTVLAELRRCGLTDSETCLKSVTLSLDGAQTVVVIKASGEVFLNQIYTQLPISAANVTIFRPSTFFIIAQTSLGLQLNLQLVPTMQLFMQLAPKLRGQTCGLCGNFNSIQADDFRTLSGVVEATAAAFFNTFKTQAACPNIRNSFEDPCSLSVENEKYAQHWCSQLTDADGP-FGRCHAAVK-PGTYYSNCMFDTCNCERSEDCLCAALSSYVHACAAKGVQLGG-WRDGVCTKPMTTCPKSMT-YHYHVSTCQPTCRSLSEGDIT-CSVGFIPVDGCICPKGTFLDDTGKCVQASNCPCYH----RGSMIPNGESVHDSGAICTCTHGKLSC-IGGQAPAPVCAAPMVFFDCRNATPGDTGAGCQKSCHTLDMTCYSPQCVPGCVCPDGLVADGEGGCITAEDCPCVHNEASYRAGQTIRVGCNTCTCDSRMWRCTDDP---CLATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQEVPYTIRQMGIYLVVDTD-IGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPS-CPDALAP---------KDPCTANPFRKSWAQKQCSILH--GPTFAAC--HAHVEPARYYEACVNDACACDSGGDCECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWHYQPCGVPCLRTC----RNPRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVCTYNGQRFHPGD 1288 HSP 2 Score: 83.9593 bits (206), Expect = 2.795e-15 Identity = 113/430 (26.28%), Postives = 161/430 (37.44%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAP--SDVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTCK--PDDQILANQQCSKIR-LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLC---------ETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVG------RC-----ICGSGEVEYGYTCIKPESCP-CLYDGQEHPPGTVRQFD--CNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDV 1940 TC YG +Y TFD Q Y++ G C Y L + S +V EN T+ T C K I I G +K S + V TD + +P Y +RQ L+ + L WD + + G V GLCG ++ + S +V FGNS+K CPD C P + A +QCS + F C V Y+ CV + C + + CT ++AY+ +C + G C+ WR+ ++C E QC + + C C TCRN P C G +C I +++ TC P P C G+ + PG V D C +C C CT K C T++G +F DV Sbjct: 900 LATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKV-------------EVIGTDESQEVP----YTIRQMGIYLVVDTDIGLVLLWD-KKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACACDSGGD--------CECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWH--YQPCGVPCLRTCRN----PRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVC--------TYNGQRFHPGDV 1289 HSP 3 Score: 74.7146 bits (182), Expect = 1.906e-12 Identity = 100/422 (23.70%), Postives = 164/422 (38.86%), Query Frame = 3 Query: 3303 NCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPI----TISVANIACGFSLA-TCAKFVQIIIGGQTYLLSRG------------NNDTI-PLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKP--SFQGNVQGLCGNYDGDLQNE-------------YMHGTMKYEDAIQFA------------ESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKW--RTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC---VEGCSCPNGM--IDLNGECVPPTTCP-CF-YNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 C+ VC +GDPHY T DG ++F +C Y L+ I P+ + V N CG +C + + + +L+R NN + P F K IV+ ++ +G++V++ S ++ P F N +G CG D ++E M G Q A + + + + P + V P+ C +P +C ++ + VF C + + +Y C++D C D + +C+SL +A CA I + W RT ++CP C K Y C P C G+++ S + EGC CP GM + + PT CP C +G+ G + M+ C++C C A + T Sbjct: 4916 QCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLV-------QQIVPVYGHFRVLVDNYFCGAEDGLSCPRSIILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNGIVVSRIGVKMYATIPELGVQVMF--SGLIFSVEVPFSKFANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVG-STTVGPTTPPAPCLPSP--------ICQLI--LSKVFEPCHTVIPPLL--FYEGCVFDRCHMT---DLDVVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKVYQPCGPSNPSYC--YGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGPHGEPVKVGHTVGMD-CQECTCEAATWTLT 5309
BLAST of SCO-spondin vs. Ensembl Human
Match: MUC5AC (mucin 5AC, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7515]) HSP 1 Score: 386.726 bits (992), Expect = 3.548e-108 Identity = 344/1299 (26.48%), Postives = 543/1299 (41.80%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMY--VLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV--EYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHT------TKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT-GSCDNTPQSIVDP-------PAGDNDICSTI--LNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD--------SVSGC-NSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTP-GDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDI--VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-----EGG--KHYDACASPCPQTCDTIGSNAPSHC-STTHCVEGC--SCPNGMIDLNG---ECV-----PPTTCPCFYNGQSYVNGAIIKME-NCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDD 4463 N C ++G +Y+TFD ++ + G C+Y+ + + ++I + ++ +V++ G V+ + G+ ++ + + L P S S ++I + Y V + +L+ ++ L+ D GLCG + + FGN K D P + C ++ F GC LV+ +Y C Q++C + +C L+ YS C+ G Q WR C +CP N + +C S C TC N Q S C++ V C C G V + G T C+ C C+Y+G + PG DC CTC+ W C E CP TC ++G F TFDG ++ + C YV +P +SA+ ++ + S+ LK+ + L +QT V I T+LP + + I I + + L + ++++++ L+ GLCG F+ +DF + + ++ T +C N S DP CS + +GP F C + K +Y+ + + + C+A+ + AC G+Q + + + C T + TCQ TCR L+E + C P GC CP + TG+CV S C C++ + IP G ++ + C C +G+L+C PVC V+ D + +GC SC D + C CP+ V+ +G C+ DCPC++ Y I + C C +RMW + C A C YGD HY T DGQ +SF G C YTL+ G + + N+ CG + TC+K ++I +GG LS G + I ++ I Q+ + + +D IGL +LWD+ T +++ L P F+G V GLCGN+D N++ + D ++F S+ + CP ++P +D C ANP RKSWA++ C ++ FA+C H + RYY C+ DAC C+ GGDCEC CT++A +A C +G+ V WRT ++CP+ C EG HY C PC +TC N C +EGC CP + +CV PP C +G+SY GA++ + NC+ C C + CT C+ T +F D Sbjct: 76 HNGRVCSTWGSFHYKTFDGDVFRFPGLCNYVFSEHCGAAYEDFNIQLRRSQESAAPTLSRVLMKVDGVVIQLTKGSVLV---NGHPVLLPFS----QSGVLIQQSSSYTKVEARLGLVLMWNHDDSLLLELDTKYA-----------NKTCGLCGDFNGMPVVSELLSHNTKLTPMEFGNLQKMDDPTDQCQDPVPEPPRNCSTGFGICEELLHGQLFSGCVALVDVGSYLEACRQDLCFCEDTD--------LLSCVCHTLAEYSRQCTHAGGLPQDWRGPDFCPQKCPNNMQYHECRSPCADTCSN--QEHSRACEDHCVAGCFCPEGTVLDDIGQTGCVPVSKCACVYNGAAYAPGATYSTDCTNCTCSGGRWSCQEVPCPGTCSVLGGAHFSTFDGKQYTVHG-DCSYVLTKPC-------------------------DSSAFTVLAELRRCGLTDSETCLKSVTLSLDGAQTVVVIKASGEVFLNQIYTQLPISAANVTIFRPSTFFIIAQTSLGLQLNLQLVPTMQLFMQLAPKLRGQTCGLCGNFNSIQADDFRTLSGVVEATAAAFFNTFKTQAACPNIRNSFEDPCSLSVENEKYAQHWCSQLTDADGP-FGRCHAAVK-PGTYYSNCMFDTCNCERSEDCLCAALSSYVHACAAKGVQLGG-WRDGVCTKPMTTCPKSMT-YHYHVSTCQPTCRSLSEGDIT-CSVGFIPVDGCICPKGTFLDDTGKCVQASNCPCYH----RGSMIPNGESVHDSGAICTCTHGKLSC-IGGQAPAPVCAAPMVFFDCRNATPGDTGAGCQKSCHTLDMTCYSPQCVPGCVCPDGLVADGEGGCITAEDCPCVHNEASYRAGQTIRVGCNTCTCDSRMWRCTDDP---CLATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKVEVIGTDESQEVPYTIRQMGIYLVVDTD-IGLVLLWDKKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPS-CPDALAP---------KDPCTANPFRKSWAQKQCSILH--GPTFAAC--HAHVEPARYYEACVNDACACDSGGDCECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWHYQPCGVPCLRTC----RNPRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVCTYNGQRFHPGD 1288 HSP 2 Score: 83.9593 bits (206), Expect = 2.795e-15 Identity = 113/430 (26.28%), Postives = 161/430 (37.44%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAP--SDVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTCK--PDDQILANQQCSKIR-LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLC---------ETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVG------RC-----ICGSGEVEYGYTCIKPESCP-CLYDGQEHPPGTVRQFD--CNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDV 1940 TC YG +Y TFD Q Y++ G C Y L + S +V EN T+ T C K I I G +K S + V TD + +P Y +RQ L+ + L WD + + G V GLCG ++ + S +V FGNS+K CPD C P + A +QCS + F C V Y+ CV + C + + CT ++AY+ +C + G C+ WR+ ++C E QC + + C C TCRN P C G +C I +++ TC P P C G+ + PG V D C +C C CT K C T++G +F DV Sbjct: 900 LATCAVYGDGHYLTFDGQSYSFNGDCEYTLVQNHCGGKDSTQDSFRVVTENVPCGTTGTTCSKAIKIFLGGFELKLSHGKV-------------EVIGTDESQEVP----YTIRQMGIYLVVDTDIGLVLLWD-KKTSIFINLSPEFKGRVCGLCGNFDDIAVNDFATRSRSVVGDVLEFGNSWKLSPSCPDALAPKDPCTANPFRKSWAQKQCSILHGPTFAACHAHVEPARYYEACVNDACACDSGGD--------CECFCTAVAAYAQACHEVGLCVSWRTPSICPLFCDYYNPEGQCEWH--YQPCGVPCLRTCRN----PRGDCLRDVRGLEGCYPKCPPEAPIFDEDKMQCVATCPTPPLPPRCHVHGKSYRPGAVVPSDKNCQSCLCTERGVECTYKAEACVC--------TYNGQRFHPGDV 1289 HSP 3 Score: 74.7146 bits (182), Expect = 1.906e-12 Identity = 100/422 (23.70%), Postives = 164/422 (38.86%), Query Frame = 3 Query: 3303 NCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPI----TISVANIACGFSLA-TCAKFVQIIIGGQTYLLSRG------------NNDTI-PLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKP--SFQGNVQGLCGNYDGDLQNE-------------YMHGTMKYEDAIQFA------------ESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKW--RTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC---VEGCSCPNGM--IDLNGECVPPTTCP-CF-YNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 C+ VC +GDPHY T DG ++F +C Y L+ I P+ + V N CG +C + + + +L+R NN + P F K IV+ ++ +G++V++ S ++ P F N +G CG D ++E M G Q A + + + + P + V P+ C +P +C ++ + VF C + + +Y C++D C D + +C+SL +A CA I + W RT ++CP C K Y C P C G+++ S + EGC CP GM + + PT CP C +G+ G + M+ C++C C A + T Sbjct: 4916 QCQCVCSGWGDPHYITFDGTYYTFLDNCTYVLV-------QQIVPVYGHFRVLVDNYFCGAEDGLSCPRSIILEYHQDRVVLTRKPVHGVMTNEIIFNNKVVSPGFRKNGIVVSRIGVKMYATIPELGVQVMF--SGLIFSVEVPFSKFANNTEGQCGTCTNDRKDECRTPRGTVVASCSEMSGLWNVSIPDQPACHRPHPTPTTVGPTTVGSTTVGPTTVG-STTVGPTTPPAPCLPSP--------ICQLI--LSKVFEPCHTVIPPLL--FYEGCVFDRCHMT---DLDVVCSSLELYAALCASHDICIDWRGRTGHMCPFTCPADKVYQPCGPSNPSYC--YGNDSASLGALPEAGPITEGCFCPEGMTLFSTSAQVCVPTGCPRCLGPHGEPVKVGHTVGMD-CQECTCEAATWTLT 5309
BLAST of SCO-spondin vs. Ensembl Human
Match: MUC2 (mucin 2, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7512]) HSP 1 Score: 335.109 bits (858), Expect = 1.583e-92 Identity = 327/1288 (25.39%), Postives = 535/1288 (41.54%), Query Frame = 3 Query: 726 NFAGGNDVNNENFT------TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMY-----VLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA----SFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK---------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV--EYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD---------SVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-------EGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGC--SCPNGMI----DLNGECVPPTTCPCFYNGQSYVNGAIIKMEN-CKKCQCVQA 4391 + AGG+++ E T C ++G +Y+TFD ++ + G C Y + S +++++ G + IL T + + + A++ TP Y + + D + + AG + L W+ + + + + GLCG+ + S D V FGN K PD+ C+ ++ +A CS+ R + F C DLV + Y C Q+ C P +C+ ++ +S CS G WR+ TLC CPGN ++ + S C TC +L S+ C+ ++ C C G V + G + C+ C C G + PG DC C CN+ W C + CP TC + G + TFDG + F+ C YV + N++ S + + +V K +GS + L + Q N LP ++ +I + + ++ +++ + A + + GLCG F+G +DF + + A ++ SC + + DP A + ++L E S VD Y C+ + +N+D L C+A+ + A AC G+ + ++ + C VF TCQ TCR L+EA S C + P GC CP + + G+CV ++C+C++ + + AG + + CVC +GRL+C+ + C +++MD S SC + + C CP+ + G CV +CPC++ N Y+ I + C C W A C C YG HY T DG+ + F G C Y + ++++ +I N+ CG + TC+K ++I +G L + I + + LV +S G+ V+WD+ T V++ L PS++G V GLCGN+D N++ M + F S+ A CP +V+ + E C+ NP R+SWAE+ C ++K + VF+ C + D +Y C++D+C C+ GGDCEC C+++A++A +C G V WRT +LCPI C E HY+ C + +TC TI + S+ S ++ +EGC CP DL +CV C C+ Y GA + E CK C C + Sbjct: 16 SLAGGSELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFASDCRGSYKEFAVHLKRG---------------PGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVV--STPHYSPGLLIEKSDAYTKVYSRAG-LTLMWNREDALMLELDTKFR-NHTCGLCGDYNGLQSYSEFLSDGVLFSPLEFGNMQK-INQPDVV-----CEDPEEEVAPASCSEHRAECERLLTAEAFADCQDLVPLEPYLRACQQDRCR----------CPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHG-DCYYVLAKGDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVL--------LNELQVN-----------LPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTETPFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCL--CAALSSYARACTAKGVML-WGWREHVCNKDVGSC-PNSQVFLYNLTTCQQTCRSLSEAD-SHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYH----RGLYLEAGDVVVRQEERCVCRDGRLHCR-QIRLIGQSCTAPKIHMDCSNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCNTCTCKRGRWVCTQ---AVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYCGQNSSLGSFSIITENVPCGTTGVTCSKAIKIFMGRTELKLEDKHRVVIQRDEGHHVAYTTREVGQYLVVESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHMVVSSELDFGNSW-KEAPTCP-------DVSTNPEP--CSLNPHRRSWAEKQCSILKSS--VFSICHSKV--DPKPFYEACVHDSCSCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPPHECEWHYEPCGNRSFETCRTI-NGIHSNISVSY-LEGCYPRCPKDRPIYEEDLK-KCVTADKCGCYVEDTHYPPGASVPTEETCKSCVCTNS 1216 HSP 2 Score: 139.428 bits (350), Expect = 3.172e-32 Identity = 132/487 (27.10%), Postives = 218/487 (44.76%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCNSCTNYDTCT--DTSSI-----ANSCGCPNDTVSKI--DGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIA-CGFS-LATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTF---------FTQLVSDSIGLRVLWDRSTRVYLF--LKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPI---MCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC-VEGCSCPNG-MIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 CP N VY++S S C DTC+ + SS+ + C CP TV D CV S C C Y P IT C + +C+ W + C C G H T DG+ ++F G C Y L GD +++ + +A CG + TC K V ++ + ++ ++ ++ L + + + +T + +VS +IG+R+ + + LF L + QG VQGLCGN++G +++ + E FA ++ A + C ++ + D C+ N + ++AE C ++K T F C A+ D YY C YD C C D CLC +L+++A C G+ + +++C C + + + C QTC ++ S A SHC V+GC CP+ +D G CVP C C++ G Y+ + + ++C C + +C + Sbjct: 295 CPGNLVYLESGSPC-----MDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVC---KDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVL--AKGDHNDSYALL----GELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSV-LLNELQVNLPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSS-CHDKLDWLD--------DPCSLNIESANYAEHWCSLLKKTETPFGRCHSAV--DPAEYYKRCKYDTCNCQNNED--CLCAALSSYARACTAKGVMLWGWREHVCNKDVGSCPNSQVFLYNLTTCQQTCRSL-SEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYHRGL-YLEAGDVVVRQEERCVCRDGRLHCRQ 751 HSP 3 Score: 117.087 bits (292), Expect = 2.384e-25 Identity = 106/377 (28.12%), Postives = 172/377 (45.62%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN---NDTIPLFPKYD--IVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQN--EYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNAN-PDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DL-NGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 VC +G+ HYKT DG +F F G C Y D + + + + + + I T L+R N + P Y ++IE+ +T++ S + GL ++W+R + L L F+ + GLCG+Y+G LQ+ E++ D + F+ N +I+ + V E+++ A+ + ++ E++ T FA C + + + Y C D C C GGD C+C+++A F+ QC+ G P WRT LCP C G Y SPC TC + S C H ++GC CP G + D+ + CVP + C C +G Y G I +C++C C ++ C + Sbjct: 36 VCSTWGNFHYKTFDGDVFRFPGLCDYNFAS---DCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSPGLLIEKSDAYTKVYSRA-GLTLMWNREDALMLELDTKFRNHTCGLCGDYNG-LQSYSEFL------SDGVLFSPLEFGNMQ----KINQPDVVCEDPEEEVAPASCSEHRAECERLL-----TAEAFADCQDLV--PLEPYLRACQQDRCRCP-GGD-TCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCE-EHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEI-TNDCEQCVCNAGRWVCKD 384
BLAST of SCO-spondin vs. Ensembl Human
Match: MUC2 (mucin 2, oligomeric mucus/gel-forming [Source:HGNC Symbol;Acc:HGNC:7512]) HSP 1 Score: 335.109 bits (858), Expect = 4.693e-92 Identity = 327/1287 (25.41%), Postives = 540/1287 (41.96%), Query Frame = 3 Query: 726 NFAGGNDVNNENFT------TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA----SFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK---------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV--EYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCK----TKDNCTNPVCPKNQVYMD---------SVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-------EGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGC--SCPNGMI----DLNGECVPPTTCPCFYNGQSYVNGAIIKMEN-CKKCQCVQA 4391 + AGG+++ E T C ++G +Y+TFD ++ + G C Y + S +++++ G + + ++ + + +Y T L L+ + PG + + + D + + AG + L W+ + + + + GLCG+ + S D V FGN + PD+ C+ ++ +A CS+ R + F C DLV + Y C Q+ C P +C+ ++ +S CS G WR+ TLC CPGN ++ + S C TC +L S+ C+ ++ C C G V + G + C+ C C G + PG DC C CN+ W C + CP TC + G + TFDG + F+ C YV + N++ S + + +V K +GS + L + Q N LP ++ +I + + ++ +++ + A + + GLCG F+G +DF + + A ++ SC + + DP A + ++L E S VD Y C+ + +N+D L C+A+ + A AC G+ + ++ + C VF TCQ TCR L+EA S C + P GC CP + + G+CV ++C+C++ + + AG + + CVC +GRL+C+ +CT P +++MD S SC + + C CP+ + G CV +CPC++ N Y+ I + C C W A C C YG HY T DG+ + F G C Y + ++++ +I N+ CG + TC+K ++I +G L + I + + LV +S G+ V+WD+ T V++ L PS++G V GLCGN+D N++ M + F S+ A CP +V+ + E C+ NP R+SWAE+ C ++K + VF+ C + D +Y C++D+C C+ GGDCEC C+++A++A +C G V WRT +LCPI C E HY+ C + +TC TI + S+ S ++ +EGC CP DL +CV C C+ Y GA + E CK C C + Sbjct: 16 SLAGGSELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFASDCRGSYKEFAVHL-------KRGPGQAEAPAGVESILLTIKDDTIYLTRH---LAVLNGAVVSTPHYSPG--LLIEKSDAYTKVYSRAG-LTLMWNREDALMLELDTKFR-NHTCGLCGDYNGLQSYSEFLSDGVLFSPLEFGN-MQKINQPDVV-----CEDPEEEVAPASCSEHRAECERLLTAEAFADCQDLVPLEPYLRACQQDRCR----------CPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHG-DCYYVLAKGDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVL--------LNELQVN-----------LPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTETPFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCL--CAALSSYARACTAKGVML-WGWREHVCNKDVGSC-PNSQVFLYNLTTCQQTCRSLSEAD-SHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYH----RGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAP-----KIHMDCSNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCNTCTCKRGRWVCTQ---AVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYCGQNSSLGSFSIITENVPCGTTGVTCSKAIKIFMGRTELKLEDKHRVVIQRDEGHHVAYTTREVGQYLVVESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHMVVSSELDFGNSW-KEAPTCP-------DVSTNPEP--CSLNPHRRSWAEKQCSILKSS--VFSICHSKV--DPKPFYEACVHDSCSCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPPHECEWHYEPCGNRSFETCRTI-NGIHSNISVSY-LEGCYPRCPKDRPIYEEDLK-KCVTADKCGCYVEDTHYPPGASVPTEETCKSCVCTNS 1216 HSP 2 Score: 139.428 bits (350), Expect = 4.658e-32 Identity = 131/486 (26.95%), Postives = 216/486 (44.44%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCNSCTNYDTCT--DTSSI-----ANSCGCPNDTVSKI--DGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTF---------FTQLVSDSIGLRVLWDRSTRVYLF--LKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPI---MCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC-VEGCSCPNG-MIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 CP N VY++S S C DTC+ + SS+ + C CP TV D CV S C C Y P IT C + +C+ W + C C G H T DG+ ++F G C Y L GD +++ + CG + TC K V ++ + ++ ++ ++ L + + + +T + +VS +IG+R+ + + LF L + QG VQGLCGN++G +++ + E FA ++ A + C ++ + D C+ N + ++AE C ++K T F C A+ D YY C YD C C D CLC +L+++A C G+ + +++C C + + + C QTC ++ S A SHC V+GC CP+ +D G CVP C C++ G Y+ + + ++C C + +C + Sbjct: 295 CPGNLVYLESGSPC-----MDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVC---KDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAK--GDHNDS---YALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSV-LLNELQVNLPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSS-CHDKLDWLD--------DPCSLNIESANYAEHWCSLLKKTETPFGRCHSAV--DPAEYYKRCKYDTCNCQNNED--CLCAALSSYARACTAKGVMLWGWREHVCNKDVGSCPNSQVFLYNLTTCQQTCRSL-SEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYHRGL-YLEAGDVVVRQEERCVCRDGRLHCRQ 751 HSP 3 Score: 116.701 bits (291), Expect = 3.088e-25 Identity = 106/377 (28.12%), Postives = 172/377 (45.62%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN---NDTIPLFPKYD--IVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQN--EYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNAN-PDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DL-NGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 VC +G+ HYKT DG +F F G C Y D + + + + + + I T L+R N + P Y ++IE+ +T++ S + GL ++W+R + L L F+ + GLCG+Y+G LQ+ E++ D + F+ N +I+ + V E+++ A+ + ++ E++ T FA C + + + Y C D C C GGD C+C+++A F+ QC+ G P WRT LCP C G Y SPC TC + S C H ++GC CP G + D+ + CVP + C C +G Y G I +C++C C ++ C + Sbjct: 36 VCSTWGNFHYKTFDGDVFRFPGLCDYNFAS---DCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSPGLLIEKSDAYTKVYSRA-GLTLMWNREDALMLELDTKFRNHTCGLCGDYNG-LQSYSEFL------SDGVLFSPLEFGNMQ----KINQPDVVCEDPEEEVAPASCSEHRAECERLL-----TAEAFADCQDLV--PLEPYLRACQQDRCRCP-GGD-TCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCE-EHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEI-TNDCEQCVCNAGRWVCKD 384 HSP 4 Score: 74.3294 bits (181), Expect = 2.327e-12 Identity = 81/342 (23.68%), Postives = 136/342 (39.77%), Query Frame = 3 Query: 3306 CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLS-----------RGNNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNE-YMHGTMKYEDAIQFAESFIAN---ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWR--TQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMID 4274 C+ C +GDPHY T DG +S+QG+C Y L++ S +N + + N C + +C + + + Q L+ + N + L + KY + + Q + +G+ V ++ + F N +G CG ++ + + A+ ++ N CP+ S A + P + +C ++KD+ +FA C + YY C++D+C G EC SL +A CA I + WR T C + C + Y AC TC + S + T VEGC CP G ++ Sbjct: 2115 CDCYCTGWGDPHYVTFDGLYYSYQGNCTYVLVEEISPSVDNF---GVYIDNYHCDPNDKVSCPRTLIVRHETQEVLIKTVHMMPMQVQVQVNRQAVALPYKKYGLEVYQSGINYVVDIPELGVLVSYNGLSFSVRLPYHRFGNNTKGQCGTCTNTTSDDCILPSGEIVSNCEAAADQWLVNDPSKPHCPHSSSTTKRPAVTVPGGG-KTTPHKDCTPSPLCQLIKDS--LFAQCHALV--PPQHYYDACVFDSCF-MPGSSLEC--ASLQAYAALCAQQNICLDWRNHTHGACLVECPSHREYQACGPAEEPTCKSSSSQQ----NNTVLVEGCFCPEGTMN 2441
BLAST of SCO-spondin vs. Ensembl Human
Match: MUC2 (mucin 2, oligomeric mucus/gel-forming [Source:NCBI gene;Acc:4583]) HSP 1 Score: 335.109 bits (858), Expect = 7.042e-92 Identity = 328/1287 (25.49%), Postives = 540/1287 (41.96%), Query Frame = 3 Query: 726 NFAGGNDVNNENFT------TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA----SFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK---------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV--EYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCK----TKDNCTNPVCPKNQVYMD---------SVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-------EGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGC--SCPNGMI----DLNGECVPPTTCPCFYNGQSYVNGAIIKM-ENCKKCQCVQA 4391 + AGG+++ E T C ++G +Y+TFD ++ + G C Y + S +++++ G + + ++ + + +Y T L L+ + PG + + + D + + AG + L W+ + + + + GLCG+ + S D V FGN K PD+ C+ ++ +A CS+ R + F C DLV + Y C Q+ C P +C+ ++ +S CS G WR+ TLC CPGN ++ + S C TC +L S+ C+ ++ C C G V + G + C+ C C G + PG DC C CN+ W C + CP TC + G + TFDG + F+ C YV + N++ S + + +V K +GS + L + Q N LP ++ +I + + ++ +++ + A + + GLCG F+G +DF + + A ++ SC + + DP A + ++L E S VD Y C+ + +N+D L C+A+ + A AC G+ + ++ + C VF TCQ TCR L+EA S C + P GC CP + + G+CV ++C+C++ + + AG + + CVC +GRL+C+ +CT P +++MD S SC + + C CP+ + G CV +CPC++ N Y+ I + C C W A C C YG HY T DG+ + F G C Y + ++++ +I N+ CG + TC+K ++I +G L + I + + LV +S G+ V+WD+ T V++ L PS++G V GLCGN+D N++ M + F S+ A CP +V+ + E C+ NP R+SWAE+ C ++K + VF+ C + D +Y C++D+C C+ GGDCEC C+++A++A +C G V WRT +LCPI C E HY+ C + +TC TI + S+ S ++ +EGC CP DL +CV C C+ Y GA + E CK C C + Sbjct: 16 SLAGGSELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFASDCRGSYKEFAVHL-------KRGPGQAEAPAGVESILLTIKDDTIYLTRH---LAVLNGAVVSTPHYSPG--LLIEKSDAYTKVYSRAG-LTLMWNREDALMLELDTKFR-NHTCGLCGDYNGLQSYSEFLSDGVLFSPLEFGNMQK-INQPDVV-----CEDPEEEVAPASCSEHRAECERLLTAEAFADCQDLVPLEPYLRACQQDRCR----------CPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHG-DCYYVLAKGDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVL--------LNELQVN-----------LPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTETPFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCL--CAALSSYARACTAKGVML-WGWREHVCNKDVGSC-PNSQVFLYNLTTCQQTCRSLSEAD-SHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYH----RGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAP-----KIHMDCSNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCNTCTCKRGRWVCTQ---AVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYCGQNSSLGSFSIITENVPCGTTGVTCSKAIKIFMGRTELKLEDKHRVVIQRDEGHHVAYTTREVGQYLVVESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHMVVSSELDFGNSW-KEAPTCP-------DVSTNPEP--CSLNPHRRSWAEKQCSILKSS--VFSICHSKV--DPKPFYEACVHDSCSCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPPHECEWHYEPCGNRSFETCRTI-NGIHSNISVSY-LEGCYPRCPKDRPIYEEDLK-KCVTADKCGCYVEDTHYPPGASVPTEETCKSCVCTNS 1216 HSP 2 Score: 139.043 bits (349), Expect = 6.440e-32 Identity = 131/486 (26.95%), Postives = 216/486 (44.44%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCNSCTNYDTCT--DTSSI-----ANSCGCPNDTVSKI--DGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTF---------FTQLVSDSIGLRVLWDRSTRVYLF--LKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPI---MCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC-VEGCSCPNG-MIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 CP N VY++S S C DTC+ + SS+ + C CP TV D CV S C C Y P IT C + +C+ W + C C G H T DG+ ++F G C Y L GD +++ + CG + TC K V ++ + ++ ++ ++ L + + + +T + +VS +IG+R+ + + LF L + QG VQGLCGN++G +++ + E FA ++ A + C ++ + D C+ N + ++AE C ++K T F C A+ D YY C YD C C D CLC +L+++A C G+ + +++C C + + + C QTC ++ S A SHC V+GC CP+ +D G CVP C C++ G Y+ + + ++C C + +C + Sbjct: 295 CPGNLVYLESGSPC-----MDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEITNDCEQCVCNAGRWVC---KDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAK--GDHNDS---YALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSV-LLNELQVNLPHVTASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFKTASGLVEATGAGFANTWKAQSS-CHDKLDWLD--------DPCSLNIESANYAEHWCSLLKKTETPFGRCHSAV--DPAEYYKRCKYDTCNCQNNED--CLCAALSSYARACTAKGVMLWGWREHVCNKDVGSCPNSQVFLYNLTTCQQTCRSL-SEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSCYHRGL-YLEAGDVVVRQEERCVCRDGRLHCRQ 751 HSP 3 Score: 116.701 bits (291), Expect = 4.235e-25 Identity = 106/377 (28.12%), Postives = 172/377 (45.62%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN---NDTIPLFPKYD--IVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQN--EYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNAN-PDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DL-NGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 VC +G+ HYKT DG +F F G C Y D + + + + + + I T L+R N + P Y ++IE+ +T++ S + GL ++W+R + L L F+ + GLCG+Y+G LQ+ E++ D + F+ N +I+ + V E+++ A+ + ++ E++ T FA C + + + Y C D C C GGD C+C+++A F+ QC+ G P WRT LCP C G Y SPC TC + S C H ++GC CP G + D+ + CVP + C C +G Y G I +C++C C ++ C + Sbjct: 36 VCSTWGNFHYKTFDGDVFRFPGLCDYNFAS---DCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSPGLLIEKSDAYTKVYSRA-GLTLMWNREDALMLELDTKFRNHTCGLCGDYNG-LQSYSEFL------SDGVLFSPLEFGNMQ----KINQPDVVCEDPEEEVAPASCSEHRAECERLL-----TAEAFADCQDLV--PLEPYLRACQQDRCRCP-GGD-TCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVYLESGSPCMDTCSHL--EVSSLCE-EHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLYTPGQEI-TNDCEQCVCNAGRWVCKD 384 HSP 4 Score: 74.3294 bits (181), Expect = 2.675e-12 Identity = 81/342 (23.68%), Postives = 136/342 (39.77%), Query Frame = 3 Query: 3306 CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLS-----------RGNNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNE-YMHGTMKYEDAIQFAESFIAN---ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWR--TQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMID 4274 C+ C +GDPHY T DG +S+QG+C Y L++ S +N + + N C + +C + + + Q L+ + N + L + KY + + Q + +G+ V ++ + F N +G CG ++ + + A+ ++ N CP+ S A + P + +C ++KD+ +FA C + YY C++D+C G EC SL +A CA I + WR T C + C + Y AC TC + S + T VEGC CP G ++ Sbjct: 4471 CDCYCTGWGDPHYVTFDGLYYSYQGNCTYVLVEEISPSVDNF---GVYIDNYHCDPNDKVSCPRTLIVRHETQEVLIKTVHMMPMQVQVQVNRQAVALPYKKYGLEVYQSGINYVVDIPELGVLVSYNGLSFSVRLPYHRFGNNTKGQCGTCTNTTSDDCILPSGEIVSNCEAAADQWLVNDPSKPHCPHSSSTTKRPAVTVPGGG-KTTPHKDCTPSPLCQLIKDS--LFAQCHALV--PPQHYYDACVFDSCF-MPGSSLEC--ASLQAYAALCAQQNICLDWRNHTHGACLVECPSHREYQACGPAEEPTCKSSSSQQ----NNTVLVEGCFCPEGTMN 4797
BLAST of SCO-spondin vs. Ensembl Celegans
Match: lrp-2 (Low-density lipoprotein RecePtor related [Source:UniProtKB/TrEMBL;Acc:O01768]) HSP 1 Score: 84.3445 bits (207), Expect = 9.198e-16 Identity = 87/239 (36.40%), Postives = 112/239 (46.86%), Query Frame = 3 Query: 4410 DYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC---NVCDLQQF------QCISNGACIPMTKKCDGTQNCEDNSDEISCGC----------------LPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWN-----GFDEKNC 5030 D S+C TC ++FRC G CI S C+ + DC+++ DE C N L +F C + CI + KCDGT +C D SDE C Q C +RTC+ + CDG DC D SDE+NC KKC E+ C +S+ CI L KCDG+ DC DG DEH CE C +T N +CI +CDG DC + G DE +C Sbjct: 2711 DESNC--TCNENEFRCQSGACIPSKARCNHMQDCNDASDEIGCPFRNCSALNEFGMTGLINCATTSQCIHPSWKCDGTNDCYDGSDEKDCFVEFDATGGSVSAPRSCDAKTQFACLATRTCMPKHWQCDGQPDCA--DGSDEKNCDAKKCTSFEFSCESSKKCIPLEQKCDGRRDCPDGEDEHQCETECDS-------KGGNATFRCTNHRCIPMAWRCDGTDDCMDNAKSLGSDEVDC 2938 HSP 2 Score: 77.0258 bits (188), Expect = 1.695e-13 Identity = 82/235 (34.89%), Postives = 113/235 (48.09%), Query Frame = 3 Query: 4407 EDYSDCIT-TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGC----------LPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--------------KKCQM-TEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCE 5033 ED C T C P F C +G CI K+C+++ DC+N DE NC C+ +F+C S GACIP +C+ Q+C D SDEI C + I C + CI CDG DC D SDE++C + C T++ C ++ C+ + +CDGQ DC DGSDE C+ CE+ + +CI ++CDG DC +G DE CE Sbjct: 2671 EDTKYCSTRVCRPGYFNCGNGLCIPEQKVCNRINDCANFADESNC-TCNENEFRCQS-GACIPSKARCNHMQDCNDASDEIGCPFRNCSALNEFGMTGLINCATTSQCIHPSWKCDGTNDC--YDGSDEKDCFVEFDATGGSVSAPRSCDAKTQFACLATRTCMPKHWQCDGQPDCADGSDEKNCDAKKCTSFEFSCESSK---------KCIPLEQKCDGRRDCPDGEDEHQCE 2892 HSP 3 Score: 68.5514 bits (166), Expect = 5.860e-11 Identity = 68/220 (30.91%), Postives = 98/220 (44.55%), Query Frame = 3 Query: 4425 ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC---NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG----CLP-HQIVC-NISRTCIEEKKICDGNYDCGVNDKSDEENCKK------CQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC---ICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 I C+ SQF+C KCI SK C+ V +C N DE++C +CD +F+C G CI ++ CDG C D DE C CL Q C N TC++ CDG DC + +E CK +M ++ C + + L + + E EC +C + C + +CI+ +CDGV DC + DEK+C Sbjct: 1010 IVKCSVSQFQCSKTKCIIKSKRCNGVQECDNGADEEDCPRSKLCDPDEFRC-GTGLCIKQSQVCDGKMQCLDGLDEEHCNEEEKCLQGRQFRCANGKSTCLDLIFRCDGVADCEDSSDESDEFCKGNSSSTCGKMNQFMCADGK---CLRSFQLCDGFPDCLTGEDETECPPSMCDSTTHLSCLNGQ---------KCISKQLECDGVDDCGDNSDEKHC 1216 HSP 4 Score: 67.3958 bits (163), Expect = 1.380e-10 Identity = 83/239 (34.73%), Postives = 116/239 (48.54%), Query Frame = 3 Query: 4368 KKCQCVQAKFNCTEDYSDCITT--CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDL----QQFQCISNG--ACIPMTKKCDGTQNCEDNSDE----------ISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQ---MTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDE 5021 K+C VQ N D DC + C P +FRC G CI+ S++CD C + DE++CN + +QF+C +NG C+ + +CDG +CED+SDE +CG + +Q +C + C+ ++CDG DC + DE C T C N Q CI +CDG DDCGD SDE C I + + C + N +CI+ CDG DC G DE Sbjct: 1030 KRCNGVQECDN-GADEEDCPRSKLCDPDEFRCGTGLCIKQSQVCDGKMQCLDGLDEEHCNEEEKCLQGRQFRC-ANGKSTCLDLIFRCDGVADCEDSSDESDEFCKGNSSSTCGKM-NQFMCADGK-CLRSFQLCDGFPDCLTGE--DETECPPSMCDSTTHLSCLNGQKCISKQLECDGVDDCGDNSDEKHCTEIRLDEAALRC-QSPMYQCDGPNFKCISDKHLCDGTNDCEEGDDE 1261 HSP 5 Score: 64.3142 bits (155), Expect = 1.001e-9 Identity = 79/257 (30.74%), Postives = 111/257 (43.19%), Query Frame = 3 Query: 4434 CTPSQFRCDD------GKCIESSKICDKVTDCSNSEDEKNCN----VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-------------------GCLPHQIVCN----------------------------ISRTCIEEKKICDGNYDCGVNDKSDEENCKK-CQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 C QF C +C+ +SK+CD V DCS +DE+ C QF+C CIP+T CD ++C+D SDE++C C+P + C+ S CI +++ CDG DC D SDE+ CKK C + C+NS C+ ++ CDG+ C DGSDE C+ K+ E+ N +C G CDG+ DC +G DEK C Sbjct: 3527 CIAGQFVCGRTAQNATAQCLYASKLCDGVKDCSGGDDEEPAFCENFECTEAQFKCGDKKKCIPLTSVCDKEKDCDDGSDELNCEITSCKPDFFACFNGTAISKCIPKEFYCDGEDDCPEGQDEPDSCFGIGECTHEQFQCTSGKCIPKRRQCDGTLDC--RDGSDEKGCKKECAIV---CDNS--CVHADDLCDGKKKCNDGSDEDEEACVIKQ------------LLEKENRKC--GGFACDGIVDCEDGSDEKGC 3762 HSP 6 Score: 62.003 bits (149), Expect = 4.856e-9 Identity = 60/201 (29.85%), Postives = 85/201 (42.29%), Query Frame = 3 Query: 4425 ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCK--KCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDE 5021 I CT QF+C GKCI + CD DC + DEK C ++ + + +C+ CDG + C D SDE C+ Q++ +R C CDG DC D SDE+ C +C + N S IC + +C + E C S + + Q I + CDG +DC +G DE Sbjct: 3656 IGECTHEQFQCTSGKCIPKRRQCDGTLDCRDGSDEKGCK----KECAIVCDNSCVHADDLCDGKKKCNDGSDEDEEACVIKQLLEKENRKC--GGFACDGIVDC--EDGSDEKGCPDLECHLAS---NKSLIC------------------DGTIDCSSGKDENN-CHNNGESNFFQCSMQTIKEWQVCDGRWDCADGLDE 3826 HSP 7 Score: 53.5286 bits (127), Expect = 2.085e-6 Identity = 64/227 (28.19%), Postives = 89/227 (39.21%), Query Frame = 3 Query: 4422 CITTCTPSQFRCDDG-KCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISN------------GACIPMTKKCDGTQNCEDNSDEISC-----GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCK--KCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNC---QCINGTKQCDGVYDCWNGFDEKNCE 5033 C++ C P+ F C KCI S CD DC + EDE F CI+ C+ +K CDG ++C DE C Q C + CI +CD DC +D SDE NC+ C+ + C N + + + +C + E C T E+ C +CI +QCDG DC +G DEK C+ Sbjct: 3478 CVSECKPNDFVCTKTYKCISSWWRCDGQDDCGDGEDEGYFVEGVCPPFPCIAGQFVCGRTAQNATAQCLYASKLCDGVKDCSGGDDEEPAFCENFECTEAQFKCGDKKKCIPLTSVCDKEKDC--DDGSDELNCEITSCKPDFFAC--------FNGTAISKCIPKEFYCDGEDDCPEGQDEPDSCFGIGECTHEQFQCTSGKCIPKRRQCDGTLDCRDGSDEKGCK 3694 HSP 8 Score: 53.1434 bits (126), Expect = 2.572e-6 Identity = 38/131 (29.01%), Postives = 65/131 (49.62%), Query Frame = 3 Query: 4311 CFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRC-------DDGKCIE-SSKICDKVTDCSNSEDEKNCNVCDL--QQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQI 4673 C NG+S N M+ K+ Q +++C + + C + RC D +C++ S+ +CD + DC + DE + D+ +++C +NG CI ++ +CDG +C D SDE +CG H Sbjct: 3792 CHNNGES--NFFQCSMQTIKEWQVCDGRWDCADGLDESPEMCASRKARCFRIGYCDDKKQCLDVSTALCDGIRDCFDGSDENPIHCKDMCKGKYRC-TNGRCIDVSARCDGRDDCGDGSDEDTCGAECHHF 3919 HSP 9 Score: 51.6026 bits (122), Expect = 7.660e-6 Identity = 31/94 (32.98%), Postives = 47/94 (50.00%), Query Frame = 3 Query: 4404 TEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCS---------NSEDEKNCNV---CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEIS 4649 +++ S C TC ++F C GKC+ +CD DC + E + CN CD ++ C + C+P+ K CDG ++C D SDE S Sbjct: 71 SDEPSTCKRTCLENEFVCKTGKCLPRGYLCDSQYDCGRLPNGDRDLSDEAPELCNQPLHCDYNEYACSKSAQCVPLFKFCDGKRDCSDGSDEHS 164
BLAST of SCO-spondin vs. Ensembl Celegans
Match: lrp-1 (Low-density lipoprotein receptor-related protein [Source:UniProtKB/Swiss-Prot;Acc:Q04833]) HSP 1 Score: 82.8037 bits (203), Expect = 2.433e-15 Identity = 70/206 (33.98%), Postives = 92/206 (44.66%), Query Frame = 3 Query: 4338 NGAIIKMENCKKCQCVQAKFNCT----------------------EDYSDCI-TTCTPSQFRCDDGK-CIESSKICDKVTDCSNSEDEKNCNV----CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCL------PHQIVCNISRTCIEEKKICDGNYDCG-VNDKSDEENCK------KCQMTEYKCNNSQ-ICILLN 4829 +G+ K+E C C +F+C ED C C+ QFRC +G+ C+ CD +DC + DE +C V C QF+C+S+G CIP + KCDG Q+C+D SDE GC Q C R CI +CDG DCG +D+S E CK KC C N Q CI L+ Sbjct: 1173 DGSDEKLEMCGNATCAANQFSCANGRCIPIYWLCDGDNDCYDGTDEDKERCPPVQCSALQFRCANGRQCVPLRNHCDGQSDCEDGSDEDSCAVTAESCTPDQFKCVSSGLCIPASWKCDGQQDCDDGSDEPKFGCTSGRQCSSDQFKCGNGR-CILNNWLCDGENDCGDGSDESSERGCKTSMNARKCPFEHVACENDQETCIPLH 1377 HSP 2 Score: 82.8037 bits (203), Expect = 2.737e-15 Identity = 55/140 (39.29%), Postives = 70/140 (50.00%), Query Frame = 3 Query: 4410 DYSDC-ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-----NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCL------PHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEY 4793 D S C + C P +CDDG CI K CD+ DC N+ DE +C C +F+C +N CIP CDG +C DNSDE S C P + C SR C+ ++C+G DCG ND SDE +EY Sbjct: 3908 DESQCTLRRCLPGHRQCDDGTCIAEHKWCDRKKDCPNAADELHCEDVSRRTCSPFEFEC-ANSVCIPRKFMCDGDNDCGDNSDETSSECRSAQCDPPLRFRCAHSRLCLNILQLCNGFNDCGPNDFSDEHLSMCSSFSEY 4046 HSP 3 Score: 78.9518 bits (193), Expect = 3.794e-14 Identity = 51/140 (36.43%), Postives = 73/140 (52.14%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNV--CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC------GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQ---MTEYKCNNSQICI 4820 C+ S+FRC+DGKCI SK+CD CS+ DE C + C QC +G CI K CD ++C + +DE+ C C P + C S CI K +CDG+ DCG N C+ Q ++C +S++C+ Sbjct: 3878 CSESEFRCNDGKCIPGSKVCDGTIQCSDGLDESQCTLRRCLPGHRQC-DDGTCIAEHKWCDRKKDCPNAADELHCEDVSRRTCSPFEFECANS-VCIPRKFMCDGDNDCGDNSDETSSECRSAQCDPPLRFRCAHSRLCL 4015 HSP 4 Score: 74.7146 bits (182), Expect = 6.711e-13 Identity = 83/267 (31.09%), Postives = 117/267 (43.82%), Query Frame = 3 Query: 4311 CFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED-----YSD---------CITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEK----NCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLP-----HQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC----KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNC---QCINGTKQCDGVYDCWNGFDE 5021 CF+ + N IK N KKC + A++ C D YSD C +F+CD+ +CI CD DC + DEK C QF C +NG CIP+ CDG +C D +DE C P Q C R C+ + CDG DC D SDE++C + C ++KC +S +CI + K + C+ EP+ C + R ++++ C +CI CDG DC +G DE Sbjct: 1095 CFHETECAEN--TIKCRNTKKC--IPAQYGCDGDNDCGDYSDEDVKYCKDGQKPVCAAKKFQCDNHRCIPEQWKCDSDNDCGDGSDEKLEMCGNATCAANQFSC-ANGRCIPIYWLCDGDNDCYDGTDEDKERCPPVQCSALQFRCANGRQCVPLRNHCDGQSDC--EDGSDEDSCAVTAESCTPDQFKCVSSGLCIPASWK---------CDGQQDCDDGSDEPKF-GCTSGRQCSSDQFKCGNGRCILNNWLCDGENDCGDGSDE 1344 HSP 5 Score: 72.0182 bits (175), Expect = 4.331e-12 Identity = 53/148 (35.81%), Postives = 74/148 (50.00%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNV--CDLQQFQCIS----NGACIPMTKKCDGTQNCEDNSDEISC-----GCLPHQI-VCNISRTCIEEKKICDGNYDCGVNDKSDEE-----NCKKCQMTEYKCNNSQICI 4820 + C + FRC+DGKCI + CD DCS+ EDEK+C C Q+QC + + +CI ++CD +C D SDE C C + +C R C + K CDG YDC D SDE+ N C +++C + CI Sbjct: 51 SVCNENDFRCNDGKCIRTEWKCDGSGDCSDGEDEKDCPHPGCKSDQWQCDTYTWHSVSCIAEYQRCDNITDCADGSDEKDCPASTVDCSSQNVFMCADGRQCFDVSKKCDGKYDC--RDLSDEKDSCSRNHTACFQYQFRCADKTQCI 196 HSP 6 Score: 70.8626 bits (172), Expect = 1.177e-11 Identity = 48/123 (39.02%), Postives = 66/123 (53.66%), Query Frame = 3 Query: 4428 TTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC---NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKK 4775 T C QFRC D +CI+ S +CD DC++ DE + C +FQC N C P +CD +C DNSDE CG C P + C + CI++ K+CDG+ DC D +DE+ C + Sbjct: 180 TACFQYQFRCADKTQCIQKSWVCDGSKDCADGSDEPDTCEFKKCTANEFQC-KNKRCQPRKFRCDYYDDCGDNSDEDECGEYRCPPGKWNCPGTGHCIDQLKLCDGSKDCA--DGADEQQCSQ 299 HSP 7 Score: 66.2402 bits (160), Expect = 2.419e-10 Identity = 77/235 (32.77%), Postives = 112/235 (47.66%), Query Frame = 3 Query: 4377 QCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDL----QQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCN------ISRTCIEEKKICDGNYDCGVNDKSDEENCK------KCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKE--PEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKN 5027 QCV N +E +C+ P F+C DG CI CD DC+++ DE N N C+ + + +N CI KKC+ +C D SDE+ C P + C + CI + K+CDG+ DC SDE C+ + +C N+ ICI + CDG DDCGD +DE+ C+ ++ + C + +CI T QCDG DC +G+DE + Sbjct: 2778 QCVLRGSN-SEPAKECLP---PYNFQCGDGSCILLGATCDSKPDCADASDE-NPNYCNTRSCPEDYNLCTNRRCIDSAKKCNHIDDCGDGSDELDC---PSAVACAEGTFPCSNGHCINQTKVCDGHNDCHDEQVSDESLATCPGLPIDCRGVKVRCPNTNICIQPADLCDGYDDCGDKADENQLFCMNQQCAQHYVRCPSG----------RCIPETWQCDGDNDCSDGWDETH 2994 HSP 8 Score: 61.2326 bits (147), Expect = 9.902e-9 Identity = 75/226 (33.19%), Postives = 99/226 (43.81%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQ---------FQCISNGACIPMTKKCDGTQNCEDNSDEIS---CG----------CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC----KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHG-CECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 C RC G+CI + CD DCS+ DE + N D FQC N CI CDG +C D SDE S CG C + + CI +CDG+ C + E C K+C E++C+N Q CI +CDG +DC DGSDEH C +P+ C N +C+ + +CDG DC +G DEK+C Sbjct: 2961 CAQHYVRCPSGRCIPETWQCDGDNDCSDGWDETHTNCTDTAGKKICVGDYLFQC-DNLKCISRAFICDGEDDCGDGSDEHSRHGCGNRTCTDQEFHCTSNAKLAQPKYECIPRAWLCDGDVTCAGGEDESTELCKTEKKECNKGEFRCSN-QHCIHSTWECDGDNDCLDGSDEHANCTYSSCQPDFFQC----------ANHKCVPNSWKCDGNDDCEDGSDEKDC 3174 HSP 9 Score: 58.5362 bits (140), Expect = 5.873e-8 Identity = 66/204 (32.35%), Postives = 97/204 (47.55%), Query Frame = 3 Query: 4467 KCIESSKICDKVTDCSNS--EDEKNCNVCD-LQQFQCISNGACIPMTKKCDGTQNCEDNSDEI--SCG-----CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKE---PEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCE 5033 +CI + C+ DC ++ ED++ C CD + +F+C ++G CIP CD +C DNSDE+ SCG C + CN + CI K+CDG C +D DE C ++C +C++ CI + CD + DC + +DE CE + + P C N CI CDG DC + DE + E Sbjct: 3805 RCIPNWAFCNGQDDCRDNSDEDKQRCPTCDDVGEFRCATSGKCIPRRWMCDTENDCGDNSDELDASCGGTTRPCSESEFRCNDGK-CIPGSKVCDGTIQC--SDGLDESQCTLRRCLPGHRQCDDG-TCIAEHKWCDRKKDCPNAADELHCEDVSRRTCSPFEFECA----------NSVCIPRKFMCDGDNDCGDNSDETSSE 3994 HSP 10 Score: 55.4546 bits (132), Expect = 5.125e-7 Identity = 46/134 (34.33%), Postives = 60/134 (44.78%), Query Frame = 3 Query: 4449 FRCDDGK-CIESSKICDKVTDCSNSEDEK-----NCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC----GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICI 4820 F C DG+ C + SK CD DC + DEK N C QF+C CI + CDG+++C D SDE C ++ C R C K CD DCG N DE +C ++ C + CI Sbjct: 144 FMCADGRQCFDVSKKCDGKYDCRDLSDEKDSCSRNHTACFQYQFRCADKTQCIQKSWVCDGSKDCADGSDEPDTCEFKKCTANEFQCKNKR-CQPRKFRCDYYDDCGDNSDEDECGEYRCPPGKWNCPGTGHCI 276 HSP 11 Score: 53.5286 bits (127), Expect = 1.746e-6 Identity = 49/155 (31.61%), Postives = 70/155 (45.16%), Query Frame = 3 Query: 4392 KFNCTEDYSDCITTCTPSQFRC--DDGKCIESSKICDKVTDCSNSEDEKNCNVCD-----LQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC--GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC------KKCQMTEYKCNNSQ 4811 +F D C CT QF C DD KCI CD DC + DE ++C + +FQC +N C + CDG +C D+SDE +C C P C + CI + + D D+SDE + + C E++CNN++ Sbjct: 3613 QFVLLSDQKTCEPNCTERQFACGGDDAKCIPKLWYCDGEPDCRDGSDEPGESICGQRICPVGEFQC-TNHNCTRPFQICDGNDDCGDSSDEQNCDKACDPWMFKCAATGRCIPRRFT--CDGDDDCGDRSDEADTLCMSAERNCTAEEFRCNNNK 3764 HSP 12 Score: 52.7582 bits (125), Expect = 3.505e-6 Identity = 36/99 (36.36%), Postives = 48/99 (48.48%), Query Frame = 3 Query: 4557 QFQCISNGACIPMTKKCDGTQNCEDNSDEI---------SCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKK-----CQMTEYKCNNSQ 4811 QFQC NG CIP KCDG +C D SDEI C + I C ++ CI + CDG+ DCG D + CK C +++C+N + Sbjct: 1060 QFQC-KNGRCIPKEWKCDGENDCLDESDEIDEKGDKCFHETECAENTIKCRNTKKCIPAQYGCDGDNDCGDYSDEDVKYCKDGQKPVCAAKKFQCDNHR 1157
BLAST of SCO-spondin vs. Ensembl Celegans
Match: egg-2 (LDL receptor repeat-containing protein egg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q21629]) HSP 1 Score: 79.7221 bits (195), Expect = 1.058e-14 Identity = 81/222 (36.49%), Postives = 106/222 (47.75%), Query Frame = 3 Query: 4425 ITTCTPSQFRCDDG--KCIESSKICDKVTDCSNSEDEKNCNVCD---------------LQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC--GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC-ICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 ++TC+ F C I SSK CD + DCS+ DE+NC C + + C+ K C+G +NC D SDE SC C Q C+ S C+ CDG DC ++SDE NC KCQ +KC + CI + CDG DC DGSDEH C+C C E LC + CI ++ CDG +DC +G DE+NC Sbjct: 120 VSTCSGFGFACTGSIDMIIPSSKRCDGLKDCSDGSDEENCKECQSIYSCRAHIEEESEKKDKTSVLPTLICLTAEKLCNGVENCPDGSDEASCRSKCSKDQFKCSGSDACLPISVKCDGVSDC--ENESDESNCNKCQKGAHKCGKN--CIKASKVCDGIPDCDDGSDEHQCDCKTCSGSEKALC----------EDGTCIMRSQVCDGKHDCLDGIDEENC 327 HSP 2 Score: 64.3142 bits (155), Expect = 5.345e-10 Identity = 36/97 (37.11%), Postives = 54/97 (55.67%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLP------HQIVCNISRTCIEEKKIC 4718 QF CD+ KC+ESS+ CD V DC + DEK C+ C + F+C ++ C+P +C+G C D SDE C C +C+ S C++ ++C Sbjct: 424 QFTCDN-KCLESSRRCDGVWDCEDKSDEKGCDKCPSRSFKCSADKKCLPFHTRCNGVAECSDGSDEHKCSCQECLGTHHDTYMCSESNRCLKRGEVC 519 HSP 3 Score: 61.2326 bits (147), Expect = 4.650e-9 Identity = 66/208 (31.73%), Postives = 92/208 (44.23%), Query Frame = 3 Query: 4419 DCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC----------------------------------NVCDL--------QQFQCISNGA----CIPMTKKCDGTQNCEDNSDEISCGCLPHQIV-CNISRTC----IEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC 4889 DC T + C+DG CI S++CD DC + DE+NC C+L F C + G CI +K CDG +CED +DE C + +V +I TC +E + CDG +DC DKSDE+ C KC +KC+ + C+ + +C+G +C DGSDEH C C Sbjct: 288 DCKTCSGSEKALCEDGTCIMRSQVCDGKHDCLDGIDEENCPGSCSNERFSSKLKLLTCDDGNQYSEVEACSGLVEACELNCPKCDPKHTFTCPAVGGIHNKCIKRSKVCDGIFDCEDGADEKKCTPVKECVVESSIQFTCDNKCLESSRRCDGVWDC--EDKSDEKGCDKCPSRSFKCSADKKCLPFHTRCNGVAECSDGSDEHKCSC 493
BLAST of SCO-spondin vs. Ensembl Celegans
Match: egg-2 (LDL receptor repeat-containing protein egg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q21629]) HSP 1 Score: 79.7221 bits (195), Expect = 1.058e-14 Identity = 81/222 (36.49%), Postives = 106/222 (47.75%), Query Frame = 3 Query: 4425 ITTCTPSQFRCDDG--KCIESSKICDKVTDCSNSEDEKNCNVCD---------------LQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC--GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC-ICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 ++TC+ F C I SSK CD + DCS+ DE+NC C + + C+ K C+G +NC D SDE SC C Q C+ S C+ CDG DC ++SDE NC KCQ +KC + CI + CDG DC DGSDEH C+C C E LC + CI ++ CDG +DC +G DE+NC Sbjct: 120 VSTCSGFGFACTGSIDMIIPSSKRCDGLKDCSDGSDEENCKECQSIYSCRAHIEEESEKKDKTSVLPTLICLTAEKLCNGVENCPDGSDEASCRSKCSKDQFKCSGSDACLPISVKCDGVSDC--ENESDESNCNKCQKGAHKCGKN--CIKASKVCDGIPDCDDGSDEHQCDCKTCSGSEKALC----------EDGTCIMRSQVCDGKHDCLDGIDEENC 327 HSP 2 Score: 64.3142 bits (155), Expect = 5.345e-10 Identity = 36/97 (37.11%), Postives = 54/97 (55.67%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLP------HQIVCNISRTCIEEKKIC 4718 QF CD+ KC+ESS+ CD V DC + DEK C+ C + F+C ++ C+P +C+G C D SDE C C +C+ S C++ ++C Sbjct: 424 QFTCDN-KCLESSRRCDGVWDCEDKSDEKGCDKCPSRSFKCSADKKCLPFHTRCNGVAECSDGSDEHKCSCQECLGTHHDTYMCSESNRCLKRGEVC 519 HSP 3 Score: 61.2326 bits (147), Expect = 4.650e-9 Identity = 66/208 (31.73%), Postives = 92/208 (44.23%), Query Frame = 3 Query: 4419 DCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC----------------------------------NVCDL--------QQFQCISNGA----CIPMTKKCDGTQNCEDNSDEISCGCLPHQIV-CNISRTC----IEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC 4889 DC T + C+DG CI S++CD DC + DE+NC C+L F C + G CI +K CDG +CED +DE C + +V +I TC +E + CDG +DC DKSDE+ C KC +KC+ + C+ + +C+G +C DGSDEH C C Sbjct: 288 DCKTCSGSEKALCEDGTCIMRSQVCDGKHDCLDGIDEENCPGSCSNERFSSKLKLLTCDDGNQYSEVEACSGLVEACELNCPKCDPKHTFTCPAVGGIHNKCIKRSKVCDGIFDCEDGADEKKCTPVKECVVESSIQFTCDNKCLESSRRCDGVWDC--EDKSDEKGCDKCPSRSFKCSADKKCLPFHTRCNGVAECSDGSDEHKCSC 493
BLAST of SCO-spondin vs. Ensembl Celegans
Match: egg-1 (LDL receptor repeat-containing protein egg-1 [Source:UniProtKB/Swiss-Prot;Acc:Q09967]) HSP 1 Score: 68.9366 bits (167), Expect = 2.031e-11 Identity = 78/223 (34.98%), Postives = 97/223 (43.50%), Query Frame = 3 Query: 4425 ITTCTPSQFRCDDG--KCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGA----------------CIPMTKKCDGTQNCEDNSDEISC--GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCEC-ICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 ++TC+ F C I SSK CD DC + DE+NC C F C C+ CD +NC D SDE C C Q C S C+ CDG DC D SDE+NC KCQ +KC CI ++ CDG C DGSDE C+C C + LC+ CI T+ CDG DC +G DE++C Sbjct: 120 VSTCSGFGFACTGAVHMVIPSSKRCDGRRDCEDGSDEENCKECQ-SVFSCKLRPEEESKKKGRSTVQPTLICLTAQHLCDNVENCPDGSDEAVCKSSCSKDQFKCPGSNACLPLSAKCDGINDCA--DASDEKNCSKCQNNAHKCGKQ--CIKASHVCDGVAQCADGSDEQQCDCQRCSGTDKALCDDGT----------CIMRTQVCDGKKDCTDGMDEEDC 327 HSP 2 Score: 66.6254 bits (161), Expect = 1.144e-10 Identity = 35/98 (35.71%), Postives = 56/98 (57.14%), Query Frame = 3 Query: 4443 SQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCL------PHQIVCNISRTCIEEKKIC 4718 +QF+CD KC++SS+ CD V DC + DEK C+ C +C ++ C+P +C+G +C D SDE+ C C + +CN S C++ ++C Sbjct: 425 TQFKCDH-KCLDSSRRCDGVWDCEDKSDEKGCDKCPSGTIKCAADKKCLPAFTRCNGVADCSDGSDELKCSCQECLGVHSNTYMCNESNRCLKRDEVC 521 HSP 3 Score: 64.6994 bits (156), Expect = 4.805e-10 Identity = 54/164 (32.93%), Postives = 82/164 (50.00%), Query Frame = 3 Query: 4359 ENCKKCQCVQAKFNCT-----EDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG-CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEE--NCKKCQMTEYKCNNSQICIL 4823 ENCK+CQ V F+C E +T P+ C+ + +CD V +C + DE C + C QF+C + AC+P++ KCDG +C D SDE +C C + C + CI+ +CDG C D SDE+ +C++C T+ + CI+ Sbjct: 157 ENCKECQSV---FSCKLRPEEESKKKGRSTVQPTLI------CLTAQHLCDNVENCPDGSDEAVCKSSCSKDQFKCPGSNACLPLSAKCDGINDCADASDEKNCSKCQNNAHKCG--KQCIKASHVCDGVAQCA--DGSDEQQCDCQRCSGTDKALCDDGTCIM 307 HSP 4 Score: 56.225 bits (134), Expect = 1.830e-7 Identity = 43/129 (33.33%), Postives = 59/129 (45.74%), Query Frame = 3 Query: 4470 CIESSKICDKVTDCSNSEDE------KNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDE-----ENCKKCQMTEYKCNNSQICI 4820 CI K+CD DC + DE K C + QF+C + C+ +++CDG +CED SDE C C I C + C+ C+G DC +D SDE + C Y CN S C+ Sbjct: 391 CISRRKVCDGTPDCDDGADEINCTPIKECGIAKNTQFKC--DHKCLDSSRRCDGVWDCEDKSDEKGCDKCPSGTIKCAADKKCLPAFTRCNGVADC--SDGSDELKCSCQECLGVHSNTYMCNESNRCL 515 HSP 5 Score: 53.1434 bits (126), Expect = 1.829e-6 Identity = 60/229 (26.20%), Postives = 88/229 (38.43%), Query Frame = 3 Query: 4359 ENCKKCQ---------CVQAKFNCT----------EDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQ------CISNGA-----------------------------------------CIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISR--------TCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICI 4820 +NC KCQ C++A C E DC + CDDG CI +++CD DC++ DE++C C ++ F S+G CI K CDGT +C+D +DEI+C + C I++ C++ + CDG +DC DKSDE+ C KC KC + C+ Sbjct: 249 KNCSKCQNNAHKCGKQCIKASHVCDGVAQCADGSDEQQCDCQRCSGTDKALCDDGTCIMRTQVCDGKKDCTDGMDEEDCPGSCTIESFSPKLKMVTCSDGKQYTEAEACSGSFESCDHDCPNSKCHPKLAFTCPASKEARKMCISRRKVCDGTPDCDDGADEINCTPIKE---CGIAKNTQFKCDHKCLDSSRRCDGVWDC--EDKSDEKGCDKCPSGTIKCAADKKCL 472
BLAST of SCO-spondin vs. Ensembl Fly
Match: Hml (gene:FBgn0029167 transcript:FBtr0333020) HSP 1 Score: 334.339 bits (856), Expect = 4.596e-92 Identity = 329/1308 (25.15%), Postives = 547/1308 (41.82%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCT--SDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXX-----XXXXXXXXXXXXXVASFGNSFK----------------GFGC-----PDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ--WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEP---KIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG-SCDNTPQSIVDP---------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCS-AILNA-AIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC---WNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCP---------KNQVYMD----SVSGCNSCTNYDT-CTDTSSIANSCGCPNDTVSKIDG-TCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGG---QTYLLSRG-----NNDTIPLFPKYDIV---------IEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGG-KHYDACASPCP-QTCDTIGSN--APSHCSTTHCVEGCS---CPNGMIDLNG---ECVPPTTCP---CFYNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 C ++G +TFD ++ CS+ L V+ + +I+ + C S GC + I +LYT ++ N L+ + + M V+ + + LE+ +KL WD+ + + ++ G V GLCG + S V +F ++++ FG + +S C +++LAN+ K C N D C+ + C + + C ++ + C+ KG ++ WR+ +C C +++ C TC + P++ + + C C G V+Y CI E CPC G+E P + + +CNTCTC + W CTE C + CG +G + TFDG ++ F C Y ++ NT A + +ESM N A ++P K VT++ I+ T + Q I + KLP + + +R+ ++ + V+ + ++++DG +YI +L+ GLCG F+ N+++DF + + ++ A + T +C ++ P A C IL F C V+ E + C + + KD++S C IL+A C R G++ + PL G VF C C +C +L + C +C GC CP Y G+CV + C C RP E+ G C C +G +C+ + P Q Y + + +C N D D+S C C V CV P++C C + + Y + I C C W CE+ C +GD H+ T DG F FQG+C Y L D+ + +I++ N+ CG TC+K ++I + G ++ LLS + + P+ D V I + F + + L+V WD TRVY+ L ++ V GLCGNY+G+ ++ +M ++ + +A +++ P + AP D C +P+R++WA+ CG +K + +F C H +V R++ C++D C C++GGDCECLCT++A +A CA GI ++WR+Q+ CP+ C+ Y AC C +TCD A C+ +C+EGC C +G I LN +CVP C +G+++ G I ++C C+C + K C+ Sbjct: 484 CTTWGGINMKTFDGLVFKAPLSCSHTLI----TDKVSGTFDIILKACPYGSGYGCAHTLKI---------LWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMW-GKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKLQKAVSVC---ERLLANE-------KLGDCIKPFNYDALIRTCMADYCNCANREHP-------ESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCDSDLALPAS--KGACNEGCFCPEGTVQYKEACITRELCPCSLRGKEFKPESTVKKNCNTCTCKNGQWRCTEDKCGARCGAVGDPHYQTFDGKRYDFMG-KCSYHLLK--TQNTSVEAENVACSGAVSESM-----NFAAPDDPSCTKAVTIRF----ILRDGTPSVIKLDQGLTTIVNDKPIAKLPKMLGLGEVLIRRASSTFLTVEFADGIRVWWDGVSRVYIDAPPSLRGQTQGLCGTFNSNTQDDFLTPEGDVETAVEPFADKWRTKDTCQFKAETHQGPHPCTLNPEKKAQAEKFCDWILQD-IFQDC--HFLVEPEQFYEDCLYDTCACKDEMSKCFCPILSAYGTECMRQGVKTGWRMSVKECAVKCPL----GQVFDECGDGCALSCDDL--PSKGSCKRECVEGCRCPHGEYVNEDGECVPKKMCHCNFDGMSFRPGYKEVRPGEKFL---DLCTCTDGVWDCQDAEPGDKDKYPPSSELRSKCAKQPYAEFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSCHHAGKSYDDGEKIKEDCNLCECRAGNWKC---SKNGCESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDGF---SITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSM----GLETSPMLFGHA----WKLQPHCS-APVAPIDACKKHPERETWAQLKCGALK--SDLFKEC----HAEVPLERFWKRCIFDTCACDQGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRCSKRKEICS 1713 HSP 2 Score: 127.487 bits (319), Expect = 8.785e-29 Identity = 108/382 (28.27%), Postives = 163/382 (42.67%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNND---TIPL--FPK----YDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIA--NADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGC-NRGGDCECLCTSLATFATQCAFLGIPVK--WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 A+C +G + KT DG +F SC +TL+ D + I + G+ CA ++I+ Y N T P+ P + + + Q+ +S+GL++ WD V + P G V GLCG DGD + T K ++ FA+++ +++C E S E D C + +K A +C + N CI+ + D C+ D C C NR C C ++A A +CAF GI ++ WR +CPI C G+ Y AC TCD S+ S C EGC CP G + C+ CPC G+ + + +K +NC C C ++ CTED Sbjct: 482 ALCTTWGGINMKTFDGLVFKAPLSCSHTLIT---DKVSGTFDIILKACPYGSGYG---CAHTLKILWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFG----MDSCEQSKLQK--AVSVCERLL-ANEKLGDCIKPFNYDA--LIRTCMADYCNCANREHPESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCD---SDLALPASKGACNEGCFCPEGTVQYKEACITRELCPCSLRGKEFKPESTVK-KNCNTCTCKNGQWRCTED 844 HSP 3 Score: 78.1814 bits (191), Expect = 8.444e-14 Identity = 183/766 (23.89%), Postives = 293/766 (38.25%), Query Frame = 3 Query: 795 YRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTG--CVKVIVI---EAGDVVV-----KSSGNGILYTTDS--NNPLTPLSVTNTDSALIIPG-TPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK-FPGCADLVNSDTYFS-MCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ---WRSDTLCETQCPGNKIWSDC-ASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQ------SNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKV-SSHFKLYFDGSKSIYIKVSDALK--NNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY--------ITGSC--DNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGI--QYEVPFTSNILQACIPLCQAGGTVFQI--CSRTCQSTCRELNEATA-----------SKCDDDC-----FPGCECPPNYYKTGTGQCVSESECT 2900 + TFD ++ Y G CS+IL D+ + +WSI+ V++ C+ +T C KVI I EAG+ ++ K NG +T N+P+ S + PG T + V + F + + G +K+ +S + V GLCG D+ S D N FG+S+ P C + Q A Q C+ I F C VN + + C++ C +N + C L ++ C +Q WR+ CE CP + +DC C +C N+ ++ C C G V G C+ P S +C C CNS + +++ T+D F+FN C Y+ V V+V + T G+ S +I +NG +I PQ S V +DG + K+P+ D I +R++ + + SH +L I+ ++K + + GLCG +GN+ ND + + + +++ V++ +S+ + C ++ P+ P + D C N F C VD Y + C+++ Q C A+ A C + GI + P Q C C + V++ C++ C T + L+E A K D+ C F GC CPP G GQCV E CT Sbjct: 2709 FTTFDGTVFKY-GPCSHILA--RDIHSSSWSIS-VHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQ-----PGKTLLAVSTKYGFWVQLDDIGIVKV-----------GISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWYDKRIPKDQCGDLKCPREMQAKALQLCNIIHHPTFARCHKAVNYKQFLNNYCLEAACNCMMANNG-----DPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDACFPGCYCPEGTVRKGPNCV-PIS------------------ECKDCVCNS---------------LGASKYMTYDRKSFSFNG-NCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHI---------LNGDHVIHVQRVPQKPKS-LQVLVDGFE-VKKIPYK--DSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDL-QPNPAKKKAGVDVIQSWQADEPKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKC--PLAVDPIAYVSACQQDICKPGNTQQGVCVALAAYAKECNQHGICTNWRRP------QLCPYECPS-DMVYEPCGCAKNC-DTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDG-GQCVPEIACT 3389 HSP 4 Score: 75.8702 bits (185), Expect = 4.421e-13 Identity = 100/410 (24.39%), Postives = 158/410 (38.54%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTL---MDTPGDSSNNIPPITISVANIACGFSL--ATCAKFVQIIIGGQTYLLSRGNNDTIPLFPK-YDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRV-----YLFLKPSFQ--------------GNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGS-----------NAPSH-------CSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQC-VQAKFNCTE 4409 VC + G Y T D + FSF G+C Y L + PG + + + + +CAK + I+ G + R +P PK ++++ DS I LR + + + ++ L SF+ ++GLCG+ +G+ N+ K + + +S+ A+ P E ++ ++ C P K Q +F C A+ D Y S C D C G + +C +LA +A +C GI WR LCP C Y+ C C + CDTI + A H C ++ EGC CP G + G+CVP C +G ++ K + C +CQC + K C E Sbjct: 3033 VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQR-----VPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVDVIQSWQADE---PKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAE-----LFGKCPLAV--DPIAYVSACQQDIC--KPGNTQQGVCVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCG--CAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGL-HLPDEKWKKDKCTECQCDSKGKTTCVE 3422 HSP 5 Score: 57.3806 bits (137), Expect = 1.793e-7 Identity = 78/314 (24.84%), Postives = 137/314 (43.63%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENC-TSDTGCVKVIVI----EAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTCK--PDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQC-PGNKIWSDCASECPV-TCRNLF 1658 +TC +G ++ TFD + +QG C Y+L + +SI I C T C K + I A + ++ S+ + Y+TD N TP+ DS ++ + F+++ + +++KWD +++ + N V+GLCG + D+ + + FG+++K C + CK P+ + A +C ++ F C V + ++ C+ + C Q + + LCT ++AY+D+C+ KG I+WRS C QC P + C C V TC N Sbjct: 1335 STCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDGFSITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSA--YSTDPNK--TPIKKLR-DSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQ-KVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPIDACKKHPERETWAQLKCGALKSDLFKECHAEVPLERFWKRCIFDTCACDQGGDC--------ECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFL 1634 HSP 6 Score: 55.4546 bits (132), Expect = 6.704e-7 Identity = 83/347 (23.92%), Postives = 129/347 (37.18%), Query Frame = 3 Query: 3342 YKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFV---------QIIIGGQTYLLSRGNNDTIPLFP-----KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMH--QDVNRYYSNCLYDACGC---NRGGDCECLCTSLATFATQCAFLGIPVK---WRTQNLCPIMCEGGK-HYDACASPCPQTCDTI-GSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 + T DG +F + G C + L SS +ISV + C K + ++I+ L G T+ K V+ Q VS G V D V + + F V GLCG Y+G+ +++ + + +F +S+ + P + + ++ A Q+C ++ + FA C +A++ Q +N Y CL AC C N G C C L +F +C + V+ WR C I C H D C +CD + G + P C GC CP G + CVP + C Sbjct: 2709 FTTFDGTVFKY-GPCSHILARDIHSSS-----WSISVHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWY-------------DKRIPKDQCGDLKCPREMQAKALQLCNIIH--HPTFARCHKAVNYKQFLNNY---CLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCP--VLPDACFPGCYCPEGTVRKGPNCVPISEC 3029 HSP 7 Score: 55.0694 bits (131), Expect = 9.460e-7 Identity = 97/413 (23.49%), Postives = 155/413 (37.53%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAV-TWSINIVYENC--------TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYV-LRQ--DNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXG-DVTGLCGX--------XXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKF------PGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWS--DCASECPVTCRNLFQTPSAYCQNSKVGR----------------CICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSS-LWHCTEKIC 1868 C+S G Y T+D + +++ G C+Y+L+ + V T+ + + ++C C K + I GD V I P + + + IP ++ LRQ ++LSL +++L + IF SI G + GLCG+ NP+ + D + S+ G + LS P + + L+F C V+ Y S C Q+IC + NT + C L+AY+ C+ G C WR LC +CP + ++ CA C T + L + + +N V C C G+V G C+ +C DG P ++ C C C+S C EK C Sbjct: 3033 VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHV-------IHVQRVPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVDVIQSWQADEPKLGL--VEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDIC---KPGNTQQGV-------CVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCGCAKNCD-TIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGLHLPDEKWKKDKCTECQCDSKGKTTCVEKKC 3425
BLAST of SCO-spondin vs. Ensembl Fly
Match: Hml (gene:FBgn0029167 transcript:FBtr0075753) HSP 1 Score: 333.954 bits (855), Expect = 5.464e-92 Identity = 329/1308 (25.15%), Postives = 547/1308 (41.82%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCT--SDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXX-----XXXXXXXXXXXXXVASFGNSFK----------------GFGC-----PDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ--WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEP---KIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG-SCDNTPQSIVDP---------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCS-AILNA-AIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC---WNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCP---------KNQVYMD----SVSGCNSCTNYDT-CTDTSSIANSCGCPNDTVSKIDG-TCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGG---QTYLLSRG-----NNDTIPLFPKYDIV---------IEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGG-KHYDACASPCP-QTCDTIGSN--APSHCSTTHCVEGCS---CPNGMIDLNG---ECVPPTTCP---CFYNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 C ++G +TFD ++ CS+ L V+ + +I+ + C S GC + I +LYT ++ N L+ + + M V+ + + LE+ +KL WD+ + + ++ G V GLCG + S V +F ++++ FG + +S C +++LAN+ K C N D C+ + C + + C ++ + C+ KG ++ WR+ +C C +++ C TC + P++ + + C C G V+Y CI E CPC G+E P + + +CNTCTC + W CTE C + CG +G + TFDG ++ F C Y ++ NT A + +ESM N A ++P K VT++ I+ T + Q I + KLP + + +R+ ++ + V+ + ++++DG +YI +L+ GLCG F+ N+++DF + + ++ A + T +C ++ P A C IL F C V+ E + C + + KD++S C IL+A C R G++ + PL G VF C C +C +L + C +C GC CP Y G+CV + C C RP E+ G C C +G +C+ + P Q Y + + +C N D D+S C C V CV P++C C + + Y + I C C W CE+ C +GD H+ T DG F FQG+C Y L D+ + +I++ N+ CG TC+K ++I + G ++ LLS + + P+ D V I + F + + L+V WD TRVY+ L ++ V GLCGNY+G+ ++ +M ++ + +A +++ P + AP D C +P+R++WA+ CG +K + +F C H +V R++ C++D C C++GGDCECLCT++A +A CA GI ++WR+Q+ CP+ C+ Y AC C +TCD A C+ +C+EGC C +G I LN +CVP C +G+++ G I ++C C+C + K C+ Sbjct: 485 CTTWGGINMKTFDGLVFKAPLSCSHTLI----TDKVSGTFDIILKACPYGSGYGCAHTLKI---------LWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMW-GKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKLQKAVSVC---ERLLANE-------KLGDCIKPFNYDALIRTCMADYCNCANREHP-------ESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCDSDLALPAS--KGACNEGCFCPEGTVQYKEACITRELCPCSLRGKEFKPESTVKKNCNTCTCKNGQWRCTEDKCGARCGAVGDPHYQTFDGKRYDFMG-KCSYHLLK--TQNTSVEAENVACSGAVSESM-----NFAAPDDPSCTKAVTIRF----ILRDGTPSVIKLDQGLTTIVNDKPIAKLPKMLGLGEVLIRRASSTFLTVEFADGIRVWWDGVSRVYIDAPPSLRGQTQGLCGTFNSNTQDDFLTPEGDVETAVEPFADKWRTKDTCQFKAETHQGPHPCTLNPEKKAQAEKFCDWILQD-IFQDC--HFLVEPEQFYEDCLYDTCACKDEMSKCFCPILSAYGTECMRQGVKTGWRMSVKECAVKCPL----GQVFDECGDGCALSCDDL--PSKGSCKRECVEGCRCPHGEYVNEDGECVPKKMCHCNFDGMSFRPGYKEVRPGEKFL---DLCTCTDGVWDCQDAEPGDKDKYPPSSELRSKCAKQPYAEFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSCHHAGKSYDDGEKIKEDCNLCECRAGNWKC---SKNGCESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDGF---SITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSM----GLETSPMLFGHA----WKLQPHCS-APVAPIDACKKHPERETWAQLKCGALK--SDLFKEC----HAEVPLERFWKRCIFDTCACDQGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDSCATCRCSKRKEICS 1714 HSP 2 Score: 127.487 bits (319), Expect = 8.639e-29 Identity = 108/382 (28.27%), Postives = 163/382 (42.67%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNND---TIPL--FPK----YDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIA--NADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGC-NRGGDCECLCTSLATFATQCAFLGIPVK--WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 A+C +G + KT DG +F SC +TL+ D + I + G+ CA ++I+ Y N T P+ P + + + Q+ +S+GL++ WD V + P G V GLCG DGD + T K ++ FA+++ +++C E S E D C + +K A +C + N CI+ + D C+ D C C NR C C ++A A +CAF GI ++ WR +CPI C G+ Y AC TCD S+ S C EGC CP G + C+ CPC G+ + + +K +NC C C ++ CTED Sbjct: 483 ALCTTWGGINMKTFDGLVFKAPLSCSHTLIT---DKVSGTFDIILKACPYGSGYG---CAHTLKILWQSVLYTFENLNGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTSRTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFG----MDSCEQSKLQK--AVSVCERLL-ANEKLGDCIKPFNYDA--LIRTCMADYCNCANREHPESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCD---SDLALPASKGACNEGCFCPEGTVQYKEACITRELCPCSLRGKEFKPESTVK-KNCNTCTCKNGQWRCTED 845 HSP 3 Score: 77.7962 bits (190), Expect = 9.579e-14 Identity = 182/766 (23.76%), Postives = 291/766 (37.99%), Query Frame = 3 Query: 795 YRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTG--CVKVIVI---EAGDVVV-----KSSGNGILYTTDS--NNPLTPLSVTNTDSALIIPG-TPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK-FPGCADLVNSDTYFS-MCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ---WRSDTLCETQCPGNKIWSDC-ASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQ------SNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKV-SSHFKLYFDGSKSIYIKVSDALK--NNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY--------ITGSC--DNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGI--QYEVPFTSNILQACIPLCQAGGTVFQI--CSRTCQSTCRELNEATA-----------SKCDDDC-----FPGCECPPNYYKTGTGQCVSESECT 2900 + TFD ++ Y G CS+IL D+ + +WSI+ V++ C+ +T C KVI I EAG+ ++ K NG +T N+P+ S + PG T + V + F + + G +K+ + V GLCG D+ S D N FG+S+ P C + Q A Q C+ I F C VN + + C++ C +N + C L ++ C +Q WR+ CE CP + +DC C +C N+ ++ C C G V G C+ P S +C C CNS + +++ T+D F+FN C Y+ V V+V + T G+ S +I +NG +I PQ S V +DG + K+P+ D I +R++ + + SH +L I+ ++K + + GLCG +GN+ ND + + + +++ V++ +S+ + C ++ P+ P + D C N F C VD Y + C+++ Q C A+ A C + GI + P Q C C + V++ C++ C T + L+E A K D+ C F GC CPP G GQCV E CT Sbjct: 2710 FTTFDGTVFKY-GPCSHILA--RDIHSSSWSIS-VHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQ-----PGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRT-----------VDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWYDKRIPKDQCGDLKCPREMQAKALQLCNIIHHPTFARCHKAVNYKQFLNNYCLEAACNCMMANNG-----DPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCPVLPDACFPGCYCPEGTVRKGPNCV-PIS------------------ECKDCVCNS---------------LGASKYMTYDRKSFSFNG-NCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHI---------LNGDHVIHVQRVPQKPKS-LQVLVDGFE-VKKIPYK--DSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDL-QPNPAKKKAGVDVIQSWQADEPKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKC--PLAVDPIAYVSACQQDICKPGNTQQGVCVALAAYAKECNQHGICTNWRRP------QLCPYECPS-DMVYEPCGCAKNC-DTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDG-GQCVPEIACT 3390 HSP 4 Score: 75.8702 bits (185), Expect = 4.728e-13 Identity = 100/410 (24.39%), Postives = 158/410 (38.54%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTL---MDTPGDSSNNIPPITISVANIACGFSL--ATCAKFVQIIIGGQTYLLSRGNNDTIPLFPK-YDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRV-----YLFLKPSFQ--------------GNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGS-----------NAPSH-------CSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQC-VQAKFNCTE 4409 VC + G Y T D + FSF G+C Y L + PG + + + + +CAK + I+ G + R +P PK ++++ DS I LR + + + ++ L SF+ ++GLCG+ +G+ N+ K + + +S+ A+ P E ++ ++ C P K Q +F C A+ D Y S C D C G + +C +LA +A +C GI WR LCP C Y+ C C + CDTI + A H C ++ EGC CP G + G+CVP C +G ++ K + C +CQC + K C E Sbjct: 3034 VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVIHVQR-----VPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVDVIQSWQADE---PKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAE-----LFGKCPLAV--DPIAYVSACQQDIC--KPGNTQQGVCVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCG--CAKNCDTIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGL-HLPDEKWKKDKCTECQCDSKGKTTCVE 3423 HSP 5 Score: 57.3806 bits (137), Expect = 1.748e-7 Identity = 78/314 (24.84%), Postives = 137/314 (43.63%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENC-TSDTGCVKVIVI----EAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTCK--PDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQC-PGNKIWSDCASECPV-TCRNLF 1658 +TC +G ++ TFD + +QG C Y+L + +SI I C T C K + I A + ++ S+ + Y+TD N TP+ DS ++ + F+++ + +++KWD +++ + N V+GLCG + D+ + + FG+++K C + CK P+ + A +C ++ F C V + ++ C+ + C Q + + LCT ++AY+D+C+ KG I+WRS C QC P + C C V TC N Sbjct: 1336 STCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDGFSITIQNVLCGTMGVTCSKSLEIALTGHAEESLLLSADSA--YSTDPNK--TPIKKLR-DSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDEGTRVYVKLGNEWRQ-KVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPIDACKKHPERETWAQLKCGALKSDLFKECHAEVPLERFWKRCIFDTCACDQGGDC--------ECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKACTPACAVETCDNFL 1635 HSP 6 Score: 55.0694 bits (131), Expect = 7.478e-7 Identity = 83/347 (23.92%), Postives = 129/347 (37.18%), Query Frame = 3 Query: 3342 YKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFV---------QIIIGGQTYLLSRGNNDTIPLFP-----KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMH--QDVNRYYSNCLYDACGC---NRGGDCECLCTSLATFATQCAFLGIPVK---WRTQNLCPIMCEGGK-HYDACASPCPQTCDTI-GSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 + T DG +F + G C + L SS +ISV + C K + ++I+ L G T+ K V+ Q VS G V D V + + F V GLCG Y+G+ +++ + + +F +S+ + P + + ++ A Q+C ++ + FA C +A++ Q +N Y CL AC C N G C C L +F +C + V+ WR C I C H D C +CD + G + P C GC CP G + CVP + C Sbjct: 2710 FTTFDGTVFKY-GPCSHILARDIHSSS-----WSISVHQQCSDETRKVCHKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISSKFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWY-------------DKRIPKDQCGDLKCPREMQAKALQLCNIIH--HPTFARCHKAVNYKQFLNNY---CLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCEPSCDNVHGDDCP--VLPDACFPGCYCPEGTVRKGPNCVPISEC 3030 HSP 7 Score: 54.6842 bits (130), Expect = 1.029e-6 Identity = 97/413 (23.49%), Postives = 155/413 (37.53%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAV-TWSINIVYENC--------TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYV-LRQ--DNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXG-DVTGLCGX--------XXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKF------PGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWS--DCASECPVTCRNLFQTPSAYCQNSKVGR----------------CICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSS-LWHCTEKIC 1868 C+S G Y T+D + +++ G C+Y+L+ + V T+ + + ++C C K + I GD V I P + + + IP ++ LRQ ++LSL +++L + IF SI G + GLCG+ NP+ + D + S+ G + LS P + + L+F C V+ Y S C Q+IC + NT + C L+AY+ C+ G C WR LC +CP + ++ CA C T + L + + +N V C C G+V G C+ +C DG P ++ C C C+S C EK C Sbjct: 3034 VCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHV-------IHVQRVPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCGDCNGNAGNDLQPNPAKKKAGVDVIQSWQADEPKLGL--VEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKCPLAVDPIAYVSACQQDIC---KPGNTQQGV-------CVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCGCAKNCD-TIKALSEFDAVSLKNEAVVHTVKTDEMCLSSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGLHLPDEKWKKDKCTECQCDSKGKTTCVEKKC 3426
BLAST of SCO-spondin vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342805) HSP 1 Score: 95.1301 bits (235), Expect = 5.837e-19 Identity = 78/221 (35.29%), Postives = 111/221 (50.23%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-----------------NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-----GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 C SQ++C DG CI K C+ + DC+++ DE NC N CD+ +F+C C+P+ KKCDG +CED SDE+ C CL + C+ C+ ++C+G +C D SDE NC C+ Y CN + C+ N +C+G DC DGSDE C I P + C N C++ +CDG+ DC +G+DE C Sbjct: 525 CLESQYQCGDGSCISGYKRCNGIHDCADASDEYNCIYDYEDTYDTDPNNNPLNECDILEFEC-DYSQCLPLEKKCDGYADCEDMSDELECQSYTDHCLESEFECD--SYCLPRDQLCNGIPNC--QDGSDERNCTFCREDAYLCNTGE-CVADNQRCNGIADCADGSDERHCARIYCPPNKLAC-----------NGTCVSRRIKCDGIRDCLDGYDEMYC 728 HSP 2 Score: 78.5666 bits (192), Expect = 6.137e-14 Identity = 73/208 (35.10%), Postives = 108/208 (51.92%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGC----ECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 + C+P QF CD+ C S C+ DCS+ DE C++ Q QC S G C +++CD ++CED SDE +C C ++ +C + CI + CDG YDC D SDE+NC C +++CNN C+ + C+G +C D SDE C EC+ P C + QC++ + +C+G DC + DE+NC Sbjct: 803 SDCSPEQFYCDE-SCYNRSVRCNGHVDCSDGSDEVGCSL-PCPQHQCPS-GRCYTESERCDRHRHCEDGSDEANCTAILCKDNEFLCFDRQFCINATQQCDGYYDC--RDFSDEQNCIGCYANQFRCNNGD-CVSGSAPCNGYSECSDHSDELNCGGTQECL---PNQFRCNSG----------QCVSSSVRCNGRTDCQDSSDEQNC 991 HSP 3 Score: 72.4034 bits (176), Expect = 4.606e-12 Identity = 48/134 (35.82%), Postives = 73/134 (54.48%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKN--CNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-GCLPH-QIVCNISR------TCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNN 4805 C ++FRC++G CI++ K C+ V+DCS EDE C ++QC CI ++++CDG +C D DE C G +P + C + +CI CDG DC ND+SDEE C C +++C++ Sbjct: 73 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRDGTRCISVSQQCDGHSDCSDGDDEEHCDGIVPKLRYTCPKGKFTCRDLSCISIVHRCDGRADC-PNDRSDEEGC-PCLYDKWQCDD 204 HSP 4 Score: 68.5514 bits (166), Expect = 7.813e-11 Identity = 49/123 (39.84%), Postives = 64/123 (52.03%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNV---CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNI------SRTCIEEKKICDGNYDCGVNDKSDEENC 4769 + CT Q C +G+CI+ S ICD DCS++ DE++C++ C QF C SN C+ T +CDG +C DNSDE SC P C S CI + CD DC D +DE C Sbjct: 1209 SACTEYQATCMNGECIDKSSICDGNPDCSDASDEQSCSLGLKCQPNQFMC-SNSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSGHCIPKSFQCDNVPDC--TDGTDEVGC 1328 HSP 5 Score: 62.7734 bits (151), Expect = 4.118e-9 Identity = 59/214 (27.57%), Postives = 88/214 (41.12%), Query Frame = 3 Query: 4431 TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEK------------NCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKK--CQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 +C P +++C + +CI+SS C+++ DCS+ DE+ + C +QF C + +C + +C+G +C D SDE+ C Q C R C E + CD + C D SDE NC C+ E+ C + Q CIN T+QCDG YDC + DE+NC Sbjct: 755 SCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYC--DESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGR-CYTESERCDRHRHC--EDGSDEANCTAILCKDNEFLCFDRQF-------------------------------------------------CINATQQCDGYYDCRDFSDEQNC 914 HSP 6 Score: 59.3066 bits (142), Expect = 4.511e-8 Identity = 70/268 (26.12%), Postives = 110/268 (41.04%), Query Frame = 3 Query: 4347 IIKMENCKKCQCVQAKFNCTEDYSDC------------ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECI-----CKE---------PEMILCXXXXXXXXXXXNCQ---------CINGTKQCDGVYDCWNGFDEKNCEI 5036 I++ E C QC+ + C + Y+DC C S+F CD C+ ++C+ + +C + DE+NC C + C + G C+ ++C+G +C D SDE C C P+++ CN TC+ + CDG DC D DE C + N + I L + ECI C E E+ +C T+ + +C C N + +C+G DC +G DE C + Sbjct: 581 ILEFE-CDYSQCLPLEKKC-DGYADCEDMSDELECQSYTDHCLESEFECD-SYCLPRDQLCNGIPNCQDGSDERNCTFCREDAYLC-NTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACN--GTCVSRRIKCDGIRDC--LDGYDEMYCPETNNHYPTQNVNVIRPKLGPNPIPKSCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSL 840 HSP 7 Score: 58.5362 bits (140), Expect = 8.540e-8 Identity = 61/212 (28.77%), Postives = 84/212 (39.62%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-------------GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 +F C + CI +CD + DC +EDE + C +++C G CIP ++ CDG C D SDE +C CL Q C +CI K C+G +DC D SDE NC Y + + NN + EC E E QC+ K+CDG DC + DE C+ + Sbjct: 441 EFACHNRDCISIESVCDGIPDCGRNEDEDDALCKCSGDKYKCQRGGGCIPKSQVCDGKPQCHDRSDESACHLHGRLNKTRLGVKCLESQYQCG-DGSCISGYKRCNGIHDCA--DASDEYNCIYDYEDTYDTDPN------NNPLN--------------ECDILEFECDYS-------------QCLPLEKKCDGYADCEDMSDELECQSY 616 HSP 8 Score: 53.5286 bits (127), Expect = 2.317e-6 Identity = 37/112 (33.04%), Postives = 51/112 (45.54%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCN-------------------------------VCDLQQFQCISNGACIPMTKKCDGTQNCE-DNSDEISCGCLPHQI 4673 C P+QFRC+ G+C+ SS C+ TDC +S DE+NC +C F+C NG CI + KC+G +C D SDE CG + + I Sbjct: 957 CLPNQFRCNSGQCVSSSVRCNGRTDCQDSSDEQNCGHRHTEVSQGLETTGVFTTSTTSTTAMTPLRIICPPTSFKC-ENGPCISLGLKCNGRVDCPYDGSDEADCGQISNDI 1067
BLAST of SCO-spondin vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342810) HSP 1 Score: 93.5893 bits (231), Expect = 1.859e-18 Identity = 78/221 (35.29%), Postives = 111/221 (50.23%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-----------------NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-----GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 C SQ++C DG CI K C+ + DC+++ DE NC N CD+ +F+C C+P+ KKCDG +CED SDE+ C CL + C+ C+ ++C+G +C D SDE NC C+ Y CN + C+ N +C+G DC DGSDE C I P + C N C++ +CDG+ DC +G+DE C Sbjct: 488 CLESQYQCGDGSCISGYKRCNGIHDCADASDEYNCIYDYEDTYDTDPNNNPLNECDILEFEC-DYSQCLPLEKKCDGYADCEDMSDELECQSYTDHCLESEFECD--SYCLPRDQLCNGIPNC--QDGSDERNCTFCREDAYLCNTGE-CVADNQRCNGIADCADGSDERHCARIYCPPNKLAC-----------NGTCVSRRIKCDGIRDCLDGYDEMYC 691 HSP 2 Score: 72.0182 bits (175), Expect = 5.490e-12 Identity = 46/112 (41.07%), Postives = 63/112 (56.25%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG----CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 Q +C G+C S+ CD+ C + DE NC C QF+C +NG C+ + C+G C D+SDE++CG CLP+Q CN S C+ C+G DC D SDE+NC Sbjct: 807 QHQCPSGRCYTESERCDRHRHCEDGSDEANC--CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCN-SGQCVSSSVRCNGRTDC--QDSSDEQNC 912 HSP 3 Score: 70.8626 bits (172), Expect = 1.209e-11 Identity = 48/134 (35.82%), Postives = 73/134 (54.48%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKN--CNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-GCLPH-QIVCNISR------TCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNN 4805 C ++FRC++G CI++ K C+ V+DCS EDE C ++QC CI ++++CDG +C D DE C G +P + C + +CI CDG DC ND+SDEE C C +++C++ Sbjct: 36 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRDGTRCISVSQQCDGHSDCSDGDDEEHCDGIVPKLRYTCPKGKFTCRDLSCISIVHRCDGRADC-PNDRSDEEGC-PCLYDKWQCDD 167 HSP 4 Score: 68.1662 bits (165), Expect = 9.877e-11 Identity = 65/188 (34.57%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 4245 GCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC----ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNV---CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNI------SRTCIEEKKICDGNYDCGVNDKSDEENC 4769 G P G D NG P + +YV A+ + Q V N E Y+D + CT Q C +G+CI+ S ICD DCS++ DE++C++ C QF C SN C+ T +CDG +C DNSDE SC P C S CI + CD DC D +DE C Sbjct: 1039 GRPLPPGFTDRNGRLEIPNIR--VEDAGTYVCEAV-GYASYIPGQQVTVNLN-VERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQSCSLGLKCQPNQFMC-SNSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSGHCIPKSFQCDNVPDC--TDGTDEVGC 1219 HSP 5 Score: 60.4622 bits (145), Expect = 1.804e-8 Identity = 67/216 (31.02%), Postives = 106/216 (49.07%), Query Frame = 3 Query: 4431 TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEK------------NCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGC----ECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 +C P +++C + +CI+SS C+++ DCS+ DE+ + C +QF C + +C + +C+G +C D SDE+ C Q C R C E + CD + C D SDE NC C +++CNN C+ + C+G +C D SDE C EC+ P C + QC++ + +C+G DC + DE+NC Sbjct: 718 SCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYC--DESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGR-CYTESERCDRHRHC--EDGSDEANC--CYANQFRCNNGD-CVSGSAPCNGYSECSDHSDELNCGGTQECL---PNQFRC----------NSGQCVSSSVRCNGRTDCQDSSDEQNC 912 HSP 6 Score: 57.7658 bits (138), Expect = 1.377e-7 Identity = 70/268 (26.12%), Postives = 110/268 (41.04%), Query Frame = 3 Query: 4347 IIKMENCKKCQCVQAKFNCTEDYSDC------------ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECI-----CKE---------PEMILCXXXXXXXXXXXNCQ---------CINGTKQCDGVYDCWNGFDEKNCEI 5036 I++ E C QC+ + C + Y+DC C S+F CD C+ ++C+ + +C + DE+NC C + C + G C+ ++C+G +C D SDE C C P+++ CN TC+ + CDG DC D DE C + N + I L + ECI C E E+ +C T+ + +C C N + +C+G DC +G DE C + Sbjct: 544 ILEFE-CDYSQCLPLEKKC-DGYADCEDMSDELECQSYTDHCLESEFECD-SYCLPRDQLCNGIPNCQDGSDERNCTFCREDAYLC-NTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACN--GTCVSRRIKCDGIRDC--LDGYDEMYCPETNNHYPTQNVNVIRPKLGPNPIPKSCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSL 803 HSP 7 Score: 56.9954 bits (136), Expect = 2.079e-7 Identity = 62/212 (29.25%), Postives = 86/212 (40.57%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-------------GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 +F C + CI +CD + DC +EDE + C +++C G CIP ++ CDG C D SDE +C CL Q C +CI K C+G +DC D SDE NC Y + + NN + EC E E C+ QC+ K+CDG DC + DE C+ + Sbjct: 404 EFACHNRDCISIESVCDGIPDCGRNEDEDDALCKCSGDKYKCQRGGGCIPKSQVCDGKPQCHDRSDESACHLHGRLNKTRLGVKCLESQYQCG-DGSCISGYKRCNGIHDCA--DASDEYNCIYDYEDTYDTDPN------NNPLN--------------ECDILEFE---CDYS----------QCLPLEKKCDGYADCEDMSDELECQSY 579 HSP 8 Score: 53.1434 bits (126), Expect = 2.954e-6 Identity = 37/112 (33.04%), Postives = 51/112 (45.54%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCN-------------------------------VCDLQQFQCISNGACIPMTKKCDGTQNCE-DNSDEISCGCLPHQI 4673 C P+QFRC+ G+C+ SS C+ TDC +S DE+NC +C F+C NG CI + KC+G +C D SDE CG + + I Sbjct: 878 CLPNQFRCNSGQCVSSSVRCNGRTDCQDSSDEQNCGHRHTEVSQGLETTGVFTTSTTSTTAMTPLRIICPPTSFKC-ENGPCISLGLKCNGRVDCPYDGSDEADCGQISNDI 988
BLAST of SCO-spondin vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342819) HSP 1 Score: 93.5893 bits (231), Expect = 1.970e-18 Identity = 78/221 (35.29%), Postives = 111/221 (50.23%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-----------------NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-----GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 C SQ++C DG CI K C+ + DC+++ DE NC N CD+ +F+C C+P+ KKCDG +CED SDE+ C CL + C+ C+ ++C+G +C D SDE NC C+ Y CN + C+ N +C+G DC DGSDE C I P + C N C++ +CDG+ DC +G+DE C Sbjct: 902 CLESQYQCGDGSCISGYKRCNGIHDCADASDEYNCIYDYEDTYDTDPNNNPLNECDILEFEC-DYSQCLPLEKKCDGYADCEDMSDELECQSYTDHCLESEFECD--SYCLPRDQLCNGIPNC--QDGSDERNCTFCREDAYLCNTGE-CVADNQRCNGIADCADGSDERHCARIYCPPNKLAC-----------NGTCVSRRIKCDGIRDCLDGYDEMYC 1105 HSP 2 Score: 72.0182 bits (175), Expect = 5.756e-12 Identity = 46/112 (41.07%), Postives = 63/112 (56.25%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG----CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 Q +C G+C S+ CD+ C + DE NC C QF+C +NG C+ + C+G C D+SDE++CG CLP+Q CN S C+ C+G DC D SDE+NC Sbjct: 1221 QHQCPSGRCYTESERCDRHRHCEDGSDEANC--CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCN-SGQCVSSSVRCNGRTDC--QDSSDEQNC 1326 HSP 3 Score: 69.3218 bits (168), Expect = 3.940e-11 Identity = 45/122 (36.89%), Postives = 64/122 (52.46%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKN--CNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG--------CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 C ++FRC++G CI++ K C+ V+DCS EDE C ++QC CI ++++CDG +C D DE C C + C CI +++CD YDC ND SDE NC Sbjct: 532 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRDGTRCISVSQQCDGHSDCSDGDDEEHCDGSGYDSEECRFDEFHCGTGE-CIPMRQVCDNIYDC--NDYSDEVNC 650 HSP 4 Score: 68.1662 bits (165), Expect = 9.280e-11 Identity = 65/188 (34.57%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 4245 GCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC----ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNV---CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNI------SRTCIEEKKICDGNYDCGVNDKSDEENC 4769 G P G D NG P + +YV A+ + Q V N E Y+D + CT Q C +G+CI+ S ICD DCS++ DE++C++ C QF C SN C+ T +CDG +C DNSDE SC P C S CI + CD DC D +DE C Sbjct: 1376 GRPLPPGFTDRNGRLEIPNIR--VEDAGTYVCEAV-GYASYIPGQQVTVNLN-VERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQSCSLGLKCQPNQFMC-SNSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSGHCIPKSFQCDNVPDC--TDGTDEVGC 1556 HSP 5 Score: 60.4622 bits (145), Expect = 2.162e-8 Identity = 67/216 (31.02%), Postives = 106/216 (49.07%), Query Frame = 3 Query: 4431 TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEK------------NCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGC----ECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 +C P +++C + +CI+SS C+++ DCS+ DE+ + C +QF C + +C + +C+G +C D SDE+ C Q C R C E + CD + C D SDE NC C +++CNN C+ + C+G +C D SDE C EC+ P C + QC++ + +C+G DC + DE+NC Sbjct: 1132 SCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYC--DESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGR-CYTESERCDRHRHC--EDGSDEANC--CYANQFRCNNGD-CVSGSAPCNGYSECSDHSDELNCGGTQECL---PNQFRC----------NSGQCVSSSVRCNGRTDCQDSSDEQNC 1326 HSP 6 Score: 56.9954 bits (136), Expect = 1.935e-7 Identity = 71/271 (26.20%), Postives = 112/271 (41.33%), Query Frame = 3 Query: 4347 IIKMENCKKCQCVQAKFNCTEDYSDC------------ITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGC---ECI-----CKE---------PEMILCXXXXXXXXXXXNCQ---------CINGTKQCDGVYDCWNGFDEKNCEI 5036 I++ E C QC+ + C + Y+DC C S+F CD C+ ++C+ + +C + DE+NC C + C + G C+ ++C+G +C D SDE C C P+++ CN TC+ + CDG DC D DE C + Y N + ++ E C ECI C E E+ +C T+ + +C C N + +C+G DC +G DE C + Sbjct: 958 ILEFE-CDYSQCLPLEKKC-DGYADCEDMSDELECQSYTDHCLESEFECD-SYCLPRDQLCNGIPNCQDGSDERNCTFCREDAYLC-NTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACN--GTCVSRRIKCDGIRDC--LDGYDEMYCPETN-NHYPTQN--VNVIRPKLGPNPIPKSCRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSL 1217 HSP 7 Score: 56.9954 bits (136), Expect = 2.195e-7 Identity = 62/212 (29.25%), Postives = 86/212 (40.57%), Query Frame = 3 Query: 4446 QFRCDDGKCIESSKICDKVTDCSNSEDEKNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC-------------GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 +F C + CI +CD + DC +EDE + C +++C G CIP ++ CDG C D SDE +C CL Q C +CI K C+G +DC D SDE NC Y + + NN + EC E E C+ QC+ K+CDG DC + DE C+ + Sbjct: 818 EFACHNRDCISIESVCDGIPDCGRNEDEDDALCKCSGDKYKCQRGGGCIPKSQVCDGKPQCHDRSDESACHLHGRLNKTRLGVKCLESQYQCG-DGSCISGYKRCNGIHDCA--DASDEYNCIYDYEDTYDTDPN------NNPLN--------------ECDILEFE---CDYS----------QCLPLEKKCDGYADCEDMSDELECQSY 993 HSP 8 Score: 54.6842 bits (130), Expect = 1.207e-6 Identity = 54/198 (27.27%), Postives = 78/198 (39.39%), Query Frame = 3 Query: 4368 KKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDG-KCIESSKICDKVTDCSNSEDEKNCN-------VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDE-------------ISCG---------------------------------------CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQ 4781 K+C V ++ +C C+ +++C DG +CI S+ CD +DCS+ +DE++C+ C +F C G CIPM + CD +C D SDE I G C +Q C S CI CDG Y C ND SDE++C++ Q Sbjct: 549 KRCNNVSDCSEGEDENEECPAACSGMEYQCRDGTRCISVSQQCDGHSDCSDGDDEEHCDGSGYDSEECRFDEFHC-GTGECIPMRQVCDNIYDCNDYSDEVNCVEGEEEDRVGIPIGHQPWRPASKHDDWLHEMDTSEYQVYQPSNVYEKANSQNPCASNQFRCTTSNVCIPLHLRCDGFYHC--NDMSDEKSCEQYQ 743
BLAST of SCO-spondin vs. Ensembl Zebrafish
Match: scospondin (subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]) HSP 1 Score: 547.74 bits (1410), Expect = 7.192e-161 Identity = 439/1471 (29.84%), Postives = 679/1471 (46.16%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXX-----XXXXXXXXXXVASFGNSFK------GFGCPDIANTLSTCKPDDQIL----ANQQCSKIR-LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQ-CPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGD--------NDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS-YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDD---CFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVY-----MDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDT---IPL-FPKY----DIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPE-NNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDG--KCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECI-CKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 +TC S+ ++RTFD + + +QG C+Y+L + +D TW++ I C + C K + + G G++ N + L+V + L G ++ L +F+ + G ++LK+D N I++++ + GLCG +NN ++ S ASFGNS++ C D A +C + + A C ++ F C V+ Y C+ C+ L++ +C L++Y C+ + I WR C T+ CP +++SDC S CP TC + + C++ VG C C G + C++ + CPC + + G + CNTC C + W C+ + C + C ++G TFD ++ C++ AVE V+ + +II + + G + + E I + L T +V ++G H LP D + R +++ + + DG ++I + + + GLCG + +DFT + + S + A +I GSC P ++ P G +CS +++ F C + VD+E + C E S Q S +CS + A C + G S P+ +GG V+Q C R C ++C +L + + + D+ C GC+CP + GQCV C C + D PAG++I NC TCVC G NC CP + ++ + + S NS D CT+ + C CP TV D CV+P +CPC + R Y ND I+ C +C + W+ A C C+A GDPHY T DG+ +SF G C Y L+ S+ + ++S N+ CG + TC K V + IG L RG T IP+ PK +++E++ F L S +G+ +LWD RVY+ L P G V GLCGN+DGD +N++ + ++ N+ ++ISP +VA +D C NP R +WA++ C V+ +F+SC H +V +YY C++DACGC+ GGDCECLCT++A +A +C G+ V+WR+Q LCP+ CE G Y+AC + C C + S + S CS CVEGC CP G + + C+ P+ CPC ++G + GA++ ++C+ C C + C + C S+FRC G +CI + +CD DC + DE C + F+C S GAC+P+ +CDG +C D SDE C P + C R C+ K+CDG DCG D SDE C C + E +C Q C+ CDG DC G DE C C + E + QC+ +CDG DC +G DEK C Sbjct: 196 STCMSWSGTHFRTFDRKHFHFQGSCTYLLASSTDG---TWAVYI-STVCETSGRCRKDLRMMLG--------LGLVSIHKDNMTVNGLAVPQGE-PLFQNGVSVHWL--GDFVFVESGLG-VRLKFDMENTIYLTVTAEHLT-NTRGLCGVYNNNPDDDFTTSTGSVSQYAASFGNSWRVQDQQQSQVCSDAAELGHSCDLSNDVSLRRDAESACHLLKETPFSHCHHQVDPGPYIDTCLYLFCS--------LEVKDRDTAVCDTLASYVRECAQQHIIISWRRRGFCGTRVCPRGQVFSDCVSSCPATCASPRPPAAGQCRDECVGGCECPQGVYLHAGQCVRRDDCPCFHRRHSYSAGQTIRQRCNTCVCRAGQWECSTEKCEAQCSVLGAMHITTFDQKRYGLQTADCRFTAVEDFVDK-----KLSIIIGAGPCAG----GEAGCLREMSITALHTT-----LTVTD------AGSVLVNG--HRNPLPVVTGDLLVR-RASSSVLLIQAFGAQLMWHLDGPL-LFITLQPGFAHKVRGLCGTLSWSQHDDFTTPEGDVENSAASFAAKFIVGSCP-APSAMTSDPCGTLTQRRQLAERVCS-VIHSNVFQACHDV--VDREPFMRLCVSEVCSCGPQASCHCSVLTAYATHCAQEGA-----LLSWRNHTFCPVQCSGGQVYQECGRLCGTSCADLWDGWSCEPDEGSRVCVSGCQCPDGLAQDAQGQCVPVHMCPC----KSGDTLHPAGSSINNNCNTCVCARGVWNCTDLSCPELNQCPGSLIFSPRSCLRTCSSPNS--PLDACTEP---IHGCICPEGTVLLGD-QCVKPDECPCHHNGRLYFTNDTISKDCNTCVCREQRWHC---GQALCSGTCVATGDPHYITFDGRHYSFLGDCEYVLVQ----ESSGL--FSVSAENVPCGSTGVTCTKSVTLTIGNTAIHLLRGKAVTVNGIPVTLPKSYSGSGLILERVGLFVSL-SSRLGVTLLWDGGMRVYVRLMPHLHGRVGGLCGNFDGDAENDFT----TRQGIMESTPELFGNS----WKISPSCPDVADQDLRDPCFINPHRVTWAKKKCAVINQE--IFSSC----HSEVPYQQYYDWCVFDACGCDSGGDCECLCTAVAAYAEECNRRGVYVRWRSQELCPLQCENGLVYEACGAACSPACPSTPSLSDSLCSALSCVEGCFCPQGTVYHDDACISPSQCPCEWDGSVFPAGAMV-TQHCQNCSCSAGLWQCVGSPCPPPSPCKDSEFRCSGGSERCIPAVWVCDNEDDCGDGSDEVCPITCPPEHFRC-SGGACLPVELRCDGHPDCADQSDEDFC---PPKFRCANGR-CVPGHKVCDGRMDCGFADDSDEYGC-VCALGELQCPGDQ-CVSAERVCDGNRDCPSGIDELICPAKGCSQFEF-----------GCTSGQCVPLAWRCDGETDCLDGSDEKRC 1555 HSP 2 Score: 55.8398 bits (133), Expect = 6.537e-7 Identity = 41/106 (38.68%), Postives = 51/106 (48.11%), Query Frame = 3 Query: 2724 CQAGGTVFQ-ICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCP 3038 C+ G VF C+ C +C +L + C PGC CPP G CV EC C P + EI G NIT++C TCVC+NG L CT+ VCP Sbjct: 4224 CEDRGRVFDGSCANQCPRSCADLWDHVQCL-QGVCHPGCRCPPGQLLQ-DGSCVPVDECRCGLPMQNGTLEIRPGENITLSCNTCVCVNGSL------VCTDHVCP 4321
BLAST of SCO-spondin vs. Ensembl Zebrafish
Match: otog (otogelin [Source:ZFIN;Acc:ZDB-GENE-120228-1]) HSP 1 Score: 337.035 bits (863), Expect = 7.730e-93 Identity = 314/1194 (26.30%), Postives = 515/1194 (43.13%), Query Frame = 3 Query: 744 DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYEN----CTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGT--PMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXV-----ASFGNSFKGFGCPDIA----------NTLSTCKPDDQILANQQCSKI-RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSD-TLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKV---GRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQF-DTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSK-SIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQ-SIVDPPAGDNDICS------TILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPP-NYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD---SVSGCNS---------CTNYD---TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLS--RGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAI------QFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCE 4142 ++ E TC S+G Y TFD Y + G+CSY L + +A + S++I N +S C + I + + L SVT ++++P + + R ++I++S + L W + + + G GLCGN + + +D V A FGNS+ P++A + +T P + + CS + R F C + V+ +Y + C ++C N V+C + Y+ +C+ + WR+ +C CP ++ +C + CP+ C+ C +SK+ C C + C+K CPC + G +P G + Q DCN CTC +W+CTE CP C + G F +FDG + F CQYV + + + V IQ+ + G + A I +V + ++I + + SQ + LP++ D +++L++++ +VK+S L ++ + +Y+++ + ++N +GLCG F+GN ++DF I + ++ ++ +C P +DP S +L FS C V + CR + L CS + + A CRR G+ E F S+ L C C + + +C CQ C L + C D+C GC CP Y+ T CV +EC C P G I + +C +G+L +T + +CP Q Y D V G ++ C Y TC+ C CP + D C +P CPC++ +EY P D ++ C + +C + + E C ++C AYGD HY+T DG +F + G C L+ + D+ +T++ N+ C S C K + I +G + G + + + I Q ++T + L VLWDR T +++ P +QG + GLCGN+D NE M+ + I +F S+ A + +I + CN NP R+ +A++ CG++ + VF C + DV +Y NCL D CGCNRGGDCEC CTS+A +A +C GIP+ WR+ ++CP CE Sbjct: 124 NLGFEREMTCRSWGQYNYETFDGLYYYFPGKCSYTLLRDCEDNARS-SVHIQVHNDPECSSSPYSCTRSI--------------SLFLPWEGEIRLQHFSVTFKGQSVLLPHHIHDVELERISDYIVVS-QPHVFTLAWQGLTSSVYIKMSPEFVGHTCGLCGNFNADTQDDLKTSYGVFTQDLAMFGNSWAEEE-PELARCTVVPSLYPSPCATLDPHVLLKVEEVCSTLLREPFQSCHEFVSPYSYMASCSNDLCLSGPNGA----------VVCQVFTEYARACAHADHPLHDWRTHFPVCNVDCPDELLFRECITCCPMHCKI-----EQMCIDSKLQCLDGCYCPDDLIFEDGVCVKASDCPCEHHGMLYPSGHIIQEDCNNCTCFGGIWNCTEHNCPGECSVTGDMFFQSFDGRVYTF-PATCQYV-----LAKSRNSGKFTVTIQN---APCGANLDGACIQSVNLVVNEDPRTEITMSHSGEVFLASQFRI---------SLPYS--DDMFQIQELSSMFVQVKLSQGLWLQYNWKEFRLYLQLEEQWRDNTLGLCGTFNGNIQDDFLAPSGMIESTPFLFGNAWRVSSACVPRPSLPQLDPCDTHQQAASYAVEKCDVLTQEVFSACHE--YVSPVGFQLQCRAD-VCKCGALCLCSILAHYARTCRRHGVIIE--FRSH-LPECAVSCPSTME-YGVCVSACQRRCLFL--SLPQPCGDECEEGCVCPEGTYFNMHTHTCVPRNECPCSFLGADYSP----GDVIITSSGVQICQDGKLVSQT--SIIETLCPAGQFYEDCGNRVDGPSASKGLACEQTCETYLLNLTCSTHEPCVPGCVCPAGLLKHGD-ECFKPRACPCLWKGKEYYPGDKVSSPCHQCVCQHGTFQC---EFRPCPSMCTAYGDRHYRTFDGFLFDYIGVCKVYLVKSSLDAM-----LTVTAENVDCFESGVICRKSLHISVGRSFIVFDDDTGRPNPSSVIDRQRAYIWQAGYYTIVHLLGEDLTVLWDRKTTIHIQAGPRWQGRLTGLCGNFDQKTANE-----MRTPENIDSSTPQEFGNSWTAAECVNSPDI-----------RHPCNLNPLREPFAKRQCGIL--LSEVFQVCHPVV--DVTWFYMNCLVDTCGCNRGGDCECFCTSVAAYAHRCCQKGIPIDWRSPSICPYDCE 1221 HSP 2 Score: 139.813 bits (351), Expect = 2.120e-32 Identity = 124/462 (26.84%), Postives = 208/462 (45.02%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCN-SCTNYDTCTDTS-SIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSL-ATCAKFVQIII------------GGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQL-VSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQ-DMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNR--YYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQ-NLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGM-IDLNGE-CVPPTTCPCFYNGQSYVNGAII 4352 CP ++ + ++ C C C D+ + C CP+D + + DG CV+ SDCPC + Y +I C C +WN NC C GD +++ DG++++F +C Y L S N T+++ N CG +L C + V +++ G+ +L S+ ++P + E + F Q+ +S + L+ W + R+YL L+ ++ N GLCG ++G++Q++++ + I+ NA + +S PS Q D C+ + S+A + C V+ T VF++C H+ V+ + C D C C CLC+ LA +A C G+ +++R+ C + C Y C S C + C + + P C C EGC CP G +++ CVP CPC + G Y G +I Sbjct: 404 CPDELLFRECITCCPMHCKIEQMCIDSKLQCLDGCYCPDDLIFE-DGVCVKASDCPCEHHGMLYPSGHIIQEDCNNCTCFGGIWNCTEH---NCPGECSVTGDMFFQSFDGRVYTFPATCQYVLAK-----SRNSGKFTVTIQNAPCGANLDGACIQSVNLVVNEDPRTEITMSHSGEVFLASQFR-ISLPYSDDMFQIQELSSMFVQVKLSQGLWLQYNW-KEFRLYLQLEEQWRDNTLGLCGTFNGNIQDDFLAPS----GMIESTPFLFGNA----WRVSSACVPRPSLPQLDPCDTHQQAASYAVEKCDVL--TQEVFSAC----HEYVSPVGFQLQCRADVCKCG----ALCLCSILAHYARTCRRHGVIIEFRSHLPECAVSCPSTMEYGVCVSACQRRC--LFLSLPQPCGD-ECEEGCVCPEGTYFNMHTHTCVPRNECPCSFLGADYSPGDVI 833 HSP 3 Score: 106.301 bits (264), Expect = 3.034e-22 Identity = 118/449 (26.28%), Postives = 193/449 (42.98%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP----LFPKY--DIVIEQLTFFTQLVSDSIGLRVLWD-RSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPEN---NVAPSKEQDMC-NANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQ-NLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHC--STTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQ---FRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISN--GACI 4589 E C ++G +Y+T DG + F G C YTL+ D++ + + I V N C S +C + + + + + + + + T L P + D+ +E+++ + +VS + W ++ VY+ + P F G+ GLCGN++ D Q++ + +D F S+ E PE V PS C +P E++C + F SC E + Y ++C D C G +C +A CA P+ WRT +C + C + C + CP C C S C++GC CP+ +I +G CV + CPC ++G Y +G II+ E+C C C +NCTE +C C+ + F+ DG+ C V S + + + Q C +N GACI Sbjct: 130 EMTCRSWGQYNYETFDGLYYYFPGKCSYTLLRDCEDNARS--SVHIQVHNDPECSSSPYSCTRSISLFLPWEGEIRLQHFSVTFKGQSVLLPHHIHDVELERISDYI-VVSQPHVFTLAWQGLTSSVYIKMSPEFVGHTCGLCGNFNADTQDDLKTSYGVFTQDLAMFGNSWA--------EEEPELARCTVVPSLYPSPCATLDPHVLLKVEEVCSTL--LREPFQSCHEFVSP--YSYMASCSNDLCLSGPNG--AVVCQVFTEYARACAHADHPLHDWRTHFPVCNVDCPDELLFRECITCCPMHC-----KIEQMCIDSKLQCLDGCYCPDDLIFEDGVCVKASDCPCEHHGMLYPSGHIIQ-EDCNNCTCFGGIWNCTEH--NCPGECSVTGDMFFQSFDGRVYTFPATCQYVLAKSRNSGKFTVTI---QNAPCGANLDGACI 550 HSP 4 Score: 62.7734 bits (151), Expect = 4.593e-9 Identity = 92/395 (23.29%), Postives = 154/395 (38.99%), Query Frame = 3 Query: 3303 NCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIP-----PITISVANIA--CGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNV--QGLCGNYDGDLQNEYM--HGTM--KYEDAIQFAESF-IANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPC--PQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI---DLNGECVPPTTCPCFYN-GQSYVNGAIIK-------MENCKKCQCVQAKFNCT 4406 +C C + D + T DG+ + + +Y + P ++ + + P V N C +L K ++++ L + P F KY I T L+ GL++ W +T + + S+ + GLCG+ DG+ N+ +GT+ + ED F +S+ I N + V+ S+ +++ D C M + N F C + V + + D N C +LA +A C I ++WR+ + CP +C G Y AC C P + P C T EGC CP G + + C+PP+ C C + G G + K M C+ V ++NC+ Sbjct: 2304 DCPCRCSVFPDLNLITFDGKSVAIYKAALYVVTQLPNETVSILVQECSGPDDTLVWNFTNLCLAALNITHKSNEVLVNRLQRRLYVNSRYAKPRFKKYGFEILD-TGNMYLIRSPAGLKIQWFHTTGMMVIEMDSYNNRLPTMGLCGSCDGNPLNDLTLSNGTVVPEQEDPAVFIDSWQIPNTT---------SYVSNSRRREVNCTTSDCS-----QCFNMLN-NNTFTLCHAYVPPGV--FCEMWVRDVEYVNNP------CIALAAYAASCHKFNICMEWRSSDFCPFVCPGNLRYQACLPACTAPSCPNQEFEFVPEQC--TGLSEGCVCPEGTLLHRPYSALCIPPSKCACTDSFGAPRALGEVWKASLDGCCMYKCENDSIVPVEYNCS 2672 HSP 5 Score: 58.151 bits (139), Expect = 1.178e-7 Identity = 41/135 (30.37%), Postives = 58/135 (42.96%), Query Frame = 3 Query: 4134 MCEGGKHYDACAS-----------PCPQTCDTIGSNAPSHCSTTH-CVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTP---SQFRCDDGKCIESSKIC 4493 +C G+ Y+ C + C QTC+T N CST CV GC CP G++ EC P CPC + G+ Y G + C +C C F C ++ C + CT +R DG + +C Sbjct: 857 LCPAGQFYEDCGNRVDGPSASKGLACEQTCETYLLNL--TCSTHEPCVPGCVCPAGLLKHGDECFKPRACPCLWKGKEYYPGDKVS-SPCHQCVCQHGTFQC--EFRPCPSMCTAYGDRHYRTFDGFLFDYIGVC 986
BLAST of SCO-spondin vs. Ensembl Zebrafish
Match: CABZ01080550.1 (pep chromosome:GRCz11:25:8269956:8292650:1 gene:ENSDARG00000100621.2 transcript:ENSDART00000166938.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01080550.1) HSP 1 Score: 324.324 bits (830), Expect = 8.907e-90 Identity = 308/1206 (25.54%), Postives = 505/1206 (41.87%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGC-VKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAG-NIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXX----XXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQ----QCSKIRL---------KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKG-SCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYT---CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIM-GTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTK------LPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGS-CDNTPQSIVDPPAGDND-------ICSTILNGPE-FSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGI---QYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD-SVSG--------CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTP-GDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDS-IGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC----EGGK---HYDACASPCPQTC 4190 + C +G +++TFD +Y + G C Y L SD ++ +I + TG + + I D+ ++ LT V ++ + +P +L ++N I L + I + W+ ++ + + + + GLCG+ N FGN + + N C+ + + Q QC K R K+ C+ +++ + Y C ++C + T LC L+ YS CS G + WR+ C QCP N + S+ S C TC + + +A C+ + C C G V + CI E C C +D + + G V + C C W C P C + G+ F TFDG +F F+ C Y+ + D + F+I+ +IV + +D LK+ + + N D T + LP+ D + +I + ++ +YI V + + GLCG F+ +D + + V+ A ++ S C + + + DP + +D CS + + F+ C + V+ + Y C+ + K + C+ + A AC G+ + ++ C P Q+ Q C RTC S E C+ D P GC CP Y+ CV +C C++ + +I G ++TI + CVC+NG+ +C++ CP +V+ + S +G +C++ + + + C CP + G CVQP +CPC + + YAP VI C K CS W C +C YG HYKT D Q F F+G C Y G+ + N +T N+ CG + TC+K V I++G LLS G + +I + LV D+ IGL VLWDR T V + L+P +V GLCGN++G+ ++++ G + + ++F +S+ CP + P + D C+ P+R++WA+ C ++K+ F C + D N YY NC+ D+C C+ GGDCEC CT++A +A C G+ V WRT +CP+ C E G+ HY C +PC +TC Sbjct: 2 SICSMWGNFHFKTFDGDVYQFPGMCEYNLV--SDCQSLIRQFSIYVKRTERSTGPKISRVSITINDIAIE---------------LTENQVNVNEAKVTLPVHVSGILVEENTIYTRLYSKMGITVMWNKDDAVMVELDSKYS-NRTCGLCGDFNGIPVYNEFIQSGRTVGYTEFGNMHR------VPNPTHQCEDPFENVDEQNVVDQCEKYRADCADLLEDEKWSSCSWVLDPEAYIKACTNDLCNRQPEDEDTT-------ALCATLTEYSRQCSHAGGNPPAWRTAKFCNVQCPYNMVHSESGSPCMDTCSH--KDTNALCEEHNIDGCFCPPGTVFDDISDTGCIPAEKCQCKHD-RVYSSGEVLRKSEEECYCQEGSWVCMSIPGPGLCAVEEGSHFTTFDGKEFTFHG-DCNYLL------SKDCEESKFIILG-------------------QIVPCFTHETDTCLKSIVVLFDNDKKNPLFIKADGTVQHNAEVSLPYMTADFTVFRPSSFHIILQTVFGLQVQVQLVPLMQVYITVDQSFQGKTCGLCGNFNKVLADDLKTPQGVVEGTAVSFANAWKAQSNCPDRSERMDDPCSYSSDSEHFAEHWCSKMKDKESLFAQC--HATVNPDSYYKRCKYSSCTCEKSEDCLCTVFSSYARACAAKGVILEGWREVVCDKYIKDC-PASQSYSYELQSCQRTCVSLASE-----RQSCNVDFVPVDGCACPDGLYQDENNLCVPMEKCPCYHNGQ----KIHPGKSLTIRDEHCVCMNGKFHCRSWKT-QILGCPSPKVFFNCSTAGPEEYGLECAKTCSHQEVDCFSLDCQSGCQCPFGLLDDGRGHCVQPYNCPCKHDGQYYAPGSVIVKDCNK--CSICRWGNWLCSQKKCPGICTIYGSGHYKTFDQQRFGFRGDCSYIAAQNKCGNKTGNFHVVT---ENMPCGTTGTTCSKAVGILLGRTWLLLSDGTVTATDVGTGPNIKYNERNVGMYLVIDAAIGLTVLWDRKTTVRIILQPQHMDDVCGLCGNFNGNGKDDFTTQGNLPTTNIMEFVDSWKV-LSTCP-------DAQP--DFDPCSQTPNRETWAKIQCSIIKEV--TFKDCHSKV--DPNPYYENCVKDSCSCDTGGDCECFCTAVAAYAQACNEAGVCVVWRTPEICPVYCDYYNEPGECTWHYSPCHTPCYKTC 1115 HSP 2 Score: 91.2781 bits (225), Expect = 9.206e-18 Identity = 96/382 (25.13%), Postives = 164/382 (42.93%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN---NDTIPLFPKY--DIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDG-DLQNEYMH-----GTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNR---GGDCECLCTSLATFATQCAFLGI-PVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 ++C +G+ H+KT DG ++ F G C Y L+ D + I +I V S V I I L+ N+ P + I++E+ T +T+L S +G+ V+W++ V + L + GLCG+++G + NE++ G ++ + + D P+E E NV D C + + +++D ++SC + D Y C D CNR D LC +L ++ QC+ G P WRT C + C + SPC TC +NA H ++GC CP G + D++ C+P C C ++ + Y +G +++ ++C C + + C Sbjct: 2 SICSMWGNFHFKTFDGDVYQFPGMCEYNLV---SDCQSLIRQFSIYVKRTE--RSTGPKISRVSITINDIAIELTENQVNVNEAKVTLPVHVSGILVEENTIYTRLYS-KMGITVMWNKDDAVMVELDSKYSNRTCGLCGDFNGIPVYNEFIQSGRTVGYTEFGNMHRVPNPTHQCED--PFENVDEQNVV-----DQC------EKYRADCADLLEDEK--WSSCSWVL--DPEAYIKACTNDL--CNRQPEDEDTTALCATLTEYSRQCSHAGGNPPAWRTAKFCNVQCPYNMVHSESGSPCMDTCSHKDTNA---LCEEHNIDGCFCPPGTVFDDISDTGCIPAEKCQCKHD-RVYSSGEVLRKSE-EECYCQEGSWVC 353
BLAST of SCO-spondin vs. Ensembl Zebrafish
Match: otogl (otogelin-like [Source:ZFIN;Acc:ZDB-GENE-050419-93]) HSP 1 Score: 319.316 bits (817), Expect = 1.931e-87 Identity = 316/1229 (25.71%), Postives = 514/1229 (41.82%), Query Frame = 3 Query: 633 FLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDV-------NNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTP--MYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSF-----KGFGCPDIANTL-----STCKPDDQILANQQCSKIRLKFP--GCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWR-SDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDG-SKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSI--VDPPAGDND------ICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPN-YYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDN-CTNPVCPKNQVYMD-------SVSGCN-----SCTNYD---TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN---NDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCE 4142 FL + E C G W G C A F G D + C S+G ++ TFD Y Y G CSYIL S +++ + + C G V + + + ++ V L +P T +++ R ++IL+ G L WD + +++ + + G GLCG +D+ S+ D++A FGNS+ + CP++ + S DD I ++CS + L FP C + ++ + + CV ++C + C L Y+ +CS G + +WR S C +C + + DC S CP TC F + C C G + TCI CPC+Y G +P G V + C C C LW+CTE C + C ++G T TFDG + + CQYV V+ N F + T G + I+ +V + + S Q+ ++ + G LP++ D+ ++VR+LT+++ ++ + +D IY+++ + +GLCG +GN +DF I + ++ S +P +I +DP + C +L F C + V Y CR Q+ C+A+ + A C + + + F +++ + C +C GG ++ C+ C TC+ + ++A CD DC GC C +Y +CV S+C C+ P G + C+C NGR+ C ++ CP +VY SG +C N TC + CGCP V +G C P +CPC ++ EY P + + C + +C +N C AVC Y D HY T DG + + C L+ + GD+ ++I N C S C K++ I +G S + + + + Y+ + ++T + + L VLWDR T V++ P ++G + GLCGN+D N+ G M+ +A F +S+ E++ + C + R+ +A++ CG++ + +FA C + DV +Y NCL D C CNRGGDCECLCTS+A +A +C G+ V WR+ +CP CE Sbjct: 16 FLFRLFVLQEYCGCLNGGWCQDEGRCDC--AQFQGLGDRCQIIPNHGQDRDGICRSWGQHHFETFDGMYYYYPGSCSYILAKDCHSSEPQYTVWV-----HNSRSC-------RGSVYSCPRSLSLFFPNEEEIYISGYQVLKGGHRLSLPQTVRNVFIERLADYILVKSTFG-FSLAWDGGSGVYLKMTEAHK-GRPCGLCGNYNDDGSDDLSSSRGIVSDDIAEFGNSWAVDLPQERPCPEVDDDFPGPCSSESDMDDAI---EKCSAL-LFFPFISCHENIDPNPFVVSCVSDMCVSDDE-----------ETFCRTLVEYTRACSHVGYPVREWRDSFPTCADRCEDSFVHRDCISCCPPTC--TFDKECLGTNLHCLDGCYCPDGLILQNGTCIAVSQCPCVYHGTAYPLGHVLEQGCTVCVCMGGLWNCTENNCTAECTVVGDTHVTTFDG-RIYMHLGSCQYVLVKSRGNTK------FTVTLQYTAC--GESQEHSCIHSVALV---------VDEDVSRQVTLTRDGEVVIGASMAVNLPYS--DETVNVRRLTSLFVGLRSVFGLRFQYDWQGGRIYLQLDSTWMGSTLGLCGTLNGNLRDDFLSPAGMIEGTPQLHVNAWKVSSACISPVNIPIIDPCEMNQQNVFYAAQCDVLLE-EVFRPC--HAYVSPSMYHQQCRY-QACRCGSSCLCTALSHYAYICSKHHVT--INFRAHVSE-CGMVC-LGGMMYHPCTSACGRTCQSV--SSAEVCDGDCAEGCSCTEGTFYDHTRQRCVLLSQCHCYFMGSVFQP----GEVSFSSSGPCLCRNGRMECVPEEREPERGDCPNGKVYYSCRGQSGRGPSGVGLACELTCRNLMLNLTCPPMTPCVPGCGCPAGLVVH-NGECYYPENCPCAWLGLEYLPGETVDTPCYRCVCHRGFFNCTYYP---CPAVCTVYSDRHYHTFDGLEYDYVSDCQVNLVKSVGDTE-----VSIMAQNKDCYESGIVCMKYLLIYVGLTKIYFSDNSGKPSSSTVVGRGYEFQLWGAGYYTVVHFPAQDLTVLWDRKTTVHIRAGPQWKGQMSGLCGNFDMVTVNDMTTAGHMEVSNAQAFGDSWAVG--------QCESDFVVHRP---CEGDLSRQPYAKRECGLLY--SDIFAPCHNVV--DVTWFYKNCLTDTCNCNRGGDCECLCTSIAAYAHKCCQNGVTVNWRSPTVCPYDCE 1153 HSP 2 Score: 131.724 bits (330), Expect = 5.043e-30 Identity = 111/396 (28.03%), Postives = 189/396 (47.73%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKFV--------QIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGT-MKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDM---CNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WR-TQNLCPIMCEGGKHYDACASPCPQTC----DTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCT 4439 + +C ++G H++T DG + + GSC Y L D ++ P T+ V N +C S+ +C + + +I I G +L G+ ++P + ++ IE+L + LV + G + WD + VYL + + +G GLCGNY+ D ++ + +D +F S+ + P+ P + D C++ D E+ ++ F SC E + D N + +C+ D C + D E C +L + C+ +G PV+ WR + C CE + C S CP TC + +G+N HC++GC CP+G+I NG C+ + CPC Y+G +Y G +++ + C C C+ +NCTE+ +C CT Sbjct: 64 DGICRSWGQHHFETFDGMYYYYPGSCSYILAK---DCHSSEPQYTVWVHNSRSCRGSVYSCPRSLSLFFPNEEEIYISGYQ-VLKGGHRLSLPQTVR-NVFIERLADYI-LVKSTFGFSLAWDGGSGVYLKMTEAHKGRPCGLCGNYNDDGSDDLSSSRGIVSDDIAEFGNSWAVDL--------PQERPCPEVDDDFPGPCSSESDMDDAIEKCSALLFFP---FISCHENI--DPNPFVVSCVSDMCVSD---DEETFCRTLVEYTRACSHVGYPVREWRDSFPTCADRCEDSFVHRDCISCCPPTCTFDKECLGTNL-------HCLDGCYCPDGLILQNGTCIAVSQCPCVYHGTAYPLGHVLE-QGCTVCVCMGGLWNCTEN--NCTAECT 427 HSP 3 Score: 55.8398 bits (133), Expect = 7.093e-7 Identity = 30/70 (42.86%), Postives = 37/70 (52.86%), Query Frame = 3 Query: 4230 THCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCT 4439 T CV GC CP G++ NGEC P CPC + G Y+ G + C +C C + FNCT Y C CT Sbjct: 830 TPCVPGCGCPAGLVVHNGECYYPENCPCAWLGLEYLPGETVDTP-CYRCVCHRGFFNCT--YYPCPAVCT 896
BLAST of SCO-spondin vs. Ensembl Zebrafish
Match: vwf (von Willebrand factor [Source:ZFIN;Acc:ZDB-GENE-070103-1]) HSP 1 Score: 318.931 bits (816), Expect = 2.738e-87 Identity = 322/1284 (25.08%), Postives = 535/1284 (41.67%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVV-VKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLR----QDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG----CPDIANTLSTCKPDDQILANQQ--CSKIR--LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLY--FDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGD-------NDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKD-QLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPV------------------CPKNQVYMDSVSG-------CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIP-PITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN-NDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK---------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGA--IIKMENCKKCQ 4379 E C + + TFD +Y + G CSY+L D ++SI + + G K + + G++ + S +G L PL P + S+ + G + +R + F + NI+L + ++ GLCGN + +++S + + F NS+ G C + +C + +Q C +R + F CA LV+ + S+C ++C S + +C + YS +C+ +G + W + + C +CP +SDC C +C+N+ C+ V CIC +G+V G C+ C C + G+ +PP + DCNTC C W CT + CP C + G + + TFD F F+ + C Y+ + D ++N F + E+ + A ++ + D+ + + G V +DG D T P N +R + + V++ L+ +DG + +++ A + GLCG ++GN +DF + + S+ + CD+ DP + + + C+ +L+ +F C V+ E Y CR + + D Q C A+ + A+AC G+ C V+++C C TCR L+ + CD C GC CP + + G+CV C+C + +P G + +I CVC +G ++C + + + + CP + S +C N + + + C CP TV + C+ CPC + +R Y IT+ C +C R W SE C+ VC A GD HY + DG FSF G C Y L D N + + V N ACG + C+K + ++ G ++ G P+ + ++ ++ ++ L+ I + WD TR+ L + ++G V GLCGN+DG N+ + + + E DA F S +++ P A S C+ + + EQ C V+ T+ +F C + D Y+ C D C C GDC C C ++A +A +CA G+PV WR+ +LCP+ CE Y+ CAS CP TC P C + CVEGC CP G + ++ +CV P+ C C ++GQ +G I+ E+ + CQ Sbjct: 24 EGSARCSLFSRQHIHTFDGVIYEFPGDCSYMLAG--DCRQRSFSILGDFSH-----GKRKGVTVFLGEMFELHLSVDGTLSQGAERLPL-PYA-----SSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHS-----------NQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGADEPCKRVLPPSQSCNSTGESTKDQMSACESMRSSVLFMHCAHLVDPAAFVSVCESDVCHCSAGK----------ECVCQAMLEYSRACASRGITLSNWHTQSQCSPKCPVGMEYSDCTPTCSTSCQNV--NIQEVCKEECVDGCICPAGKVLDGDRCVDVSQCSCTHAGRRYPPASYISQDCNTCVCRHGSWECTNEGCPGECLVTGQSHYKTFDNKFFTFSGI-CHYLLAK------DCQSNSFSAV---IETAQCADDQDAICTR----SITLRFKDLANQTVHLKHGGV---VSVDGMDVQT------PLINGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYAGQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSWKMNADCDDVQMQNSDPCSLNPKRVRFAEEACAVLLSM-KFEPC--HYMVNPEPYVKNCRYDVCACADGQECLCDAVSSYAVACAARGVLVNWRSHGYCELTC-----PDAQVYEMCGSVCNQTCRSLS-LPDTGCDGVCEEGCFCPRGLFLSDAGECVPPQHCSCHHNGEIFEPNDMIGDHHSI----CVCESGSMHCTSNEATGSTLSDLFFDEQSPSRGRRSLSCPPPLQRFECESARDAGIECAKTCQNLELDCVSLGCVSGCMCPPGTV-RYRKDCIPQEQCPCYHNSRPYESGQSITVDCNTCVCEQRQWK-CSERV--CDGVCRAIGDTHYISFDGLKFSFPGPCQYVLAQ---DYCNGLDGTFRVLVENSACGIAGYRCSKSITVLYMGGLIVMEHGEVRMKRPVLKETEVEIVRSGLYYIVLLGKHIS--ITWDTGTRISLQISGQYRGKVCGLCGNFDGSQNNDLLSSSNQMEVDAADFGNS---------WKVRPSCADAVSV-VSQCSTDLVKLVTVEQSCRVL--TSAIFRDCNSVV--DPEPYWDICTQDTCSCPSVGDCACFCNTIAAYAHECAQKGLPVNWRSNDLCPLSCEDLNKESGVQCEWRYNTCASACPSTCQ---HPEPLSCPIS-CVEGCHAVCPPGHLLDEVLRKCVEPSQCQVCVHDGQRVSHGKQFILNHEDPRLCQ 1208 HSP 2 Score: 181.8 bits (460), Expect = 3.714e-45 Identity = 148/490 (30.20%), Postives = 215/490 (43.88%), Query Frame = 3 Query: 2997 NCKTKDNCTNPVCPKNQVYMDSVSGCN-SCTNY---DTCTDTSSIANSCGCPNDTVSKIDG-TCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQII---IGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLR------VLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNR--YYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGM-IDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 N T+ C+ P CP Y D C+ SC N + C + + C CP V +DG CV S C C + R Y P I+ C +C + W +E C C+ G HYKT D + F+F G C Y L D +N I A A A C + + + + QT L G ++ +I V S+ LR + WD RV L LKP++ G + GLCGNY+G+ ++++ E ++ F S+ NAD ++ + D C+ NP R +AE+ C V+ E H VN Y NC YD C C G ECLC +++++A CA G+ V WR+ C + C + Y+ C S C QTC ++ + P C EGC CP G+ + GECVPP C C +NG+ + +I ++ C C +CT + Sbjct: 277 NWHTQSQCS-PKCPVGMEYSDCTPTCSTSCQNVNIQEVCKE--ECVDGCICPAGKV--LDGDRCVDVSQCSCTHAGRRYPPASYISQDCNTCVCRHGSWECTNE---GCPGECLVTGQSHYKTFDNKFFTFSGICHYLLAK---DCQSNSFSAVIETAQCA-DDQDAICTRSITLRFKDLANQTVHLKHGGVVSVDGMDVQTPLINGPLRIQNTVLSSVRLRYGHDLHLDWDGRGRVLLRLKPAYAGQMCGLCGNYNGNQGDDFLTVGGLVETRVEAFGNSWKMNADCDDVQM---------QNSDPCSLNPKRVRFAEEACAVLLSMK------FEPCHYMVNPEPYVKNCRYDVCACADGQ--ECLCDAVSSYAVACAARGVLVNWRSHGYCELTCPDAQVYEMCGSVCNQTCRSL--SLPDTGCDGVCEEGCFCPRGLFLSDAGECVPPQHCSCHHNGEIFEPNDMIG-DHHSICVCESGSMHCTSN 734 HSP 3 Score: 113.62 bits (283), Expect = 1.782e-24 Identity = 131/495 (26.46%), Postives = 193/495 (38.99%), Query Frame = 3 Query: 1617 DCASECPVTCRNL-FQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIIL-----KATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGDNDICST------ILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNIL--QACIPLCQAGGT----VFQICSRTCQSTCRELNEATASKCDDDCFPGCE--CPPNYYKTGT-GQCVSESEC-TCWNPSRPKDPEIPAGTNITIN------CQTCVCINGRLNC 3002 D EC TC+NL S C V C+C G V Y CI E CPC ++ + + G DCNTC C W C+E++C C +G T + +FDG KF+F CQYV + N D V V S G G K +TV G I++ + P L +++ + G +Y V + H + +D I +++S + + GLCG FDG+ ND N + + S+ + SC + S+V + D T +L F C +S VD E Y C ++ S D +C+ I A C + G+ V + SN L +C L + G + C+ C STC+ + C C GC CPP + +CV S+C C + + + G +N CQ C C + L C Sbjct: 774 DAGIECAKTCQNLELDCVSLGC----VSGCMCPPGTVRYRKDCIPQEQCPCYHNSRPYESGQSITVDCNTCVCEQRQWKCSERVCDGVCRAIGDTHYISFDGLKFSFPG-PCQYVLAQDYCNGLDGTFRVLV-----ENSACGIAGYRC----SKSITVLYMGGLIVMEHGEVRMKRPVLKETEVEIVRSG-----------------------LYYIVLLGKHISITWDTGTRISLQISGQYRGKVCGLCGNFDGSQNNDLLSSSNQMEVDAADFGNSWKVRPSCADA-VSVVSQCSTDLVKLVTVEQSCRVLTSAIFRDC--NSVVDPEPYWDICTQDTCSCPSVGDCACFCNTIAAYAHECAQKGL--PVNWRSNDLCPLSCEDLNKESGVQCEWRYNTCASACPSTCQHPEPLS---CPISCVEGCHAVCPPGHLLDEVLRKCVEPSQCQVCVHDGQ----RVSHGKQFILNHEDPRLCQICHCESNTLTC 1219 HSP 4 Score: 98.9821 bits (245), Expect = 4.523e-20 Identity = 112/427 (26.23%), Postives = 168/427 (39.34%), Query Frame = 3 Query: 3264 SNRMW----NLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYL-------LSRGNNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESF-IANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED--YSDCITTCTPSQFRCDDGKCIESSKIC 4493 S MW NL A C + H T DG ++ F G C Y L GD +I FS K V + +G L LS+G + +PL + + + +L S+ G + D + L L+ GLCGNY+ ++EY +D FA S+ + AD P V P + CN+ + C M+ ++ +F C + D + S C D C C+ G EC+C ++ ++ CA GI + W TQ+ C C G Y C C +C + N C CV+GC CP G + CV + C C + G+ Y + I ++C C C + CT + +C+ T S ++ D K S IC Sbjct: 7 SRIMWIVILNLSDLLLVEGSARCSLFSRQHIHTFDGVIYEFPGDCSYMLA---GDCRQR-------SFSILGDFSHGK-RKGVTVFLGEMFELHLSVDGTLSQGA-ERLPLPYASSSVFAGYELGYVRLWSEEFGFSIRIDPLHNIQLTLEKRHSNQTCGLCGNYNYLAEDEYSAQEGFLTDDPYDFANSWAMKGAD------EPCKRVLPPSQS--CNSTGESTKDQMSACESMR-SSVLFMHCAHLV--DPAAFVSVCESDVCHCSAGK--ECVCQAMLEYSRACASRGITLSNWHTQSQCSPKCPVGMEYSDCTPTCSTSCQNV--NIQEVCK-EECVDGCICPAGKVLDGDRCVDVSQCSCTHAGRRYPPASYIS-QDCNTCVCRHGSWECTNEGCPGECLVT-GQSHYKTFDNKFFTFSGIC 403 HSP 5 Score: 74.3294 bits (181), Expect = 1.407e-12 Identity = 106/408 (25.98%), Postives = 166/408 (40.69%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCT---SDTGCVKVI-VIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGF-GCPDIANTLSTCKPD--DQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDV--LCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPG-NK--------IWSDCASECPVTCRNLFQTPSAY-CQNSKVGRC--ICGSGEV--EYGYTCIKPESCP-CLYDGQEHPPGTVRQFD--------CNTCTCNSSLWHCTEKICPST 1877 C + G +Y +FD +++ G C Y+L A + + + ++ EN + C K I V+ G ++V G P ++ T + ++R + ++ L +I + WD I + I G V GLCG ++ S+ + A FGNS+K C D + +S C D + Q C + F C +V+ + Y+ +C Q+ C+ PS+ D C ++AY+ C+ KG + WRS+ LC C NK ++ CAS CP TC Q P C S V C +C G + E C++P C C++DGQ G +QF C C C S+ C CP T Sbjct: 857 VCRAIGDTHYISFDGLKFSFPGPCQYVL-AQDYCNGLDGTFRVLVENSACGIAGYRCSKSITVLYMGGLIVMEHGE-----VRMKRP-------------VLKETEVEIVRSGLYYIVLL-GKHISITWDTGTRISLQISGQYR-GKVCGLCGNFDGSQNNDLLSSSNQMEVDAADFGNSWKVRPSCADAVSVVSQCSTDLVKLVTVEQSCRVLTSAIFRDCNSVVDPEPYWDICTQDTCS----------CPSVGDCACFCNTIAAYAHECAQKGLPVNWRSNDLCPLSCEDLNKESGVQCEWRYNTCASACPSTC----QHPEPLSCPISCVEGCHAVCPPGHLLDEVLRKCVEPSQCQVCVHDGQRVSHG--KQFILNHEDPRLCQICHCESNTLTCGP--CPVT 1225 HSP 6 Score: 65.4698 bits (158), Expect = 7.587e-10 Identity = 87/388 (22.42%), Postives = 143/388 (36.86%), Query Frame = 3 Query: 3297 TANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWD-RSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC--------DTIGSNA----PSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCP--CFYNGQSYVNGAIIKMEN--CKKCQCV-QAKFNCT 4406 T C C+ H + G + +C Y L+ + G S N C SL +++ + G +P + V + + QL S+ G + + S L + + + N GLCG+ D + D + +++ ++ ++ E +C A E+ C V+ ++ VF+ C + V Y C AC N LC + ++T C G+ V WRT +LCP+ C Y AC S C + C D +G S C T EGC CP G + + +CV C ++G+++ E+ C C C+ Q + NCT Sbjct: 1944 TWTCPCSCMGSSTSHVVSFSGAALKVKSACSYLLLRSGGAELVLHTTACQSSPNQICMKSLELREGGTSVVLQDDMKVTVGGVLTAVPFRTERVTVTQIGSILHQLRSEQHGFTLTFTPHSNEFMLNMDIAMKPNTTGLCGSCSDDQSGLLLSDGSVTTDPDVYLQAWALGSECVQHQ-----------EIQLCPAG------LEKACDVL--SSEVFSHCHALV--PVQDYIQICRSVACQPNA------LCDLVTAYSTVCRQQGVCVDWRTSSLCPMSCSDSMEYAACRSGCLEQCGQSQFTPGDMMGDGLLKANGSVCLNTP-TEGCFCPEGSVLMEDKCVDREACSQCVEHSGKTHQFMESWNPEDNPCVLCVCLDQQRINCT 2303
BLAST of SCO-spondin vs. Ensembl Xenopus
Match: ENSXETT00000006505.1 (SCO-spondin [Source:NCBI gene;Acc:100497129]) HSP 1 Score: 571.622 bits (1472), Expect = 1.563e-168 Identity = 460/1529 (30.09%), Postives = 702/1529 (45.91%), Query Frame = 3 Query: 648 VSYDECCADKTG-SWGS-QSGSCVSCK-----------ANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXX-----XXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQIL-----ANQQCSKIR--LKFPGCADL------VNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGDNDICSTILNGPEFS----------GCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDD--DCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTI--------PLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD-DGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC---GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSD-EENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKE---PEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 +S +ECC+ G SW + S C SC G + F+TC ++G ++RTFD + + + G C+Y L + D TW++ I E C T V + + + S NG+ P V + + + I L +F+ + G +++K+D NN+++++ G GLCG + + + + A F NS++ T S C+ +L + +C+ R L P L V+ Y+ C+ C S ++ + +C ++Y+ C+ + I WR CE +CP K +SDC S CP +C + C++ V C C +G C+K E CPC + Q + PG + CN C C WHCT+ C + C ++G + TFD +++F+ C Y+ V+ V+ +I N G++G+ + + + +TV + + I L ++ + ++G + T LPF PD + VR +++ + ++ + L+ + +I + N + GLCG F+ N +DFT + I S + A + ++ C + DP C T EF+ C + V+ E + C + + NK+ L CSA+ A C + G S Q P+ GG ++ C+ C+ TC +L A C + C GC CP +GQCV + C C + + P G+ I NC++CVC NG NC CP N VY + C+S TC+DT + C CP T + +CV P DCPC + + Y PND I C +C NR W+ C VC A GDPHY T DG+ F+F G C Y L + N T++ N+ CG S TC K V ++IG + RG TI ++ I +E+ FT +S +GL VLWD TRVY+ L P+F+G V GLCGN+DGD +N++ E + + +CP E++ ++ P C N R +WA + CGV+ + +FA+C HQ+V +YY C+YD CGC+ GGDCECLCT++AT+A QC G+ ++WRTQ+LCP+ C+ G Y+AC CPQTC +G HC++ CVEGC CP G + G C+ P+ CPC++ G + GA IK CK C C K+ C + + C ++F C +C+ ++ +CD DC + DE C + +C +NG C+P+ +CDG +C D+SDE SC C ++ C+ R CI +CDG DCG D SD +C T ++C+ + C+ + CDG DDCGD SDE GC C E P+ + CI K CDG DC G DE C Sbjct: 134 MSMEECCSHPWGHSWKNITSDFCFSCSYVSPTGEPYFSTALVGVSPQQRRLFSTCVTWGGFHFRTFDGKHFHFHGTCTYTLASSLDS---TWAVYISEELCPQPTLEVTLGLERVTVRRREVSVNGVT---------VPEGVPHLQNGINI-------LWLGDFLFIESGLG-VRVKFDGRNNVYVTVTLDQR-GRTRGLCGVYNEDPQDDFTEVGGNVSPHAAGFANSWRIQDSNTQVRTNSFCQFHFSMLIYHFMPSSRCATPRAHLAKPTICFLCLFSSQVDPSGYYETCMYTYCQHSGGASDR------AGAVCETFTSYARECAQQKIIIDWRRAGFCEKKCPNGKEYSDCMSSCPASCAAVGSYEDGQCRDECVSGCECPAGLYLEQGVCVKEEECPCFHRRQRYSPGDTIKQRCNQCVCRGGRWHCTQDKCAAQCSVVGDPHYVTFDRKRYSFHGT-CDYILVQDYVDGK------LLITAENV--ACGSKGSVSCL---RAITVTAHKTSIRLS--------TKGDYTVNGREVT--LPFLSPD--LSVRWVSSSFLLLQTFGAYVLWSLEFPAAHITLQPVFSNKVRGLCGTFNWNQNDDFTTPEGDIETSSFSFANKFKVSAECPDVGPFTFDP-------CGTYAQRREFAEEACAVILSPACHD--LVEWEPFHQLCLYDVCGCAPNKNCL--CSAVSAYARQCAQEGA-----IMSWRNQTFCPVQCTGGQMYMECTPPCRKTCADLRMAGTGSCQELEGCVAGCNCPEGLVLDESGQCVPPNMCQCHHGAETHQP----GSKIQQNCKSCVCENGVWNCTGSSCPEAAYCPGNMVYSPGSCLLTCDSLERNGTCSDT---LDGCVCPEGTA--LLDSCVLPEDCPCHHHGKLYHPNDTIVKDCNTCVCKNRHWSCTD---LQCSGVCTATGDPHYVTFDGRSFTFLGDCQYVL------ARENGGLFTVTAENVPCGTSGITCTKSVVVMIGNMIVHMLRGKEVTINGISVRPPKIYSGNGITLERAGLFTMFISQ-MGLTVLWDGGTRVYVKLDPAFRGRVSGLCGNFDGDTENDFTSRQGIVEPTADLFGNSWRMSLMCP-EVNADDFEHP------CTENSHRVTWARKKCGVLLQS--LFAAC----HQEVPCQQYYDWCVYDTCGCDSGGDCECLCTAIATYAEQCNQRGVYIRWRTQDLCPMQCDNGMVYEACGPACPQTCKNLGLAPDPHCNSLSCVEGCFCPAGKVLHEGRCLDPSDCPCYWGGMPFPTGAAIK-HGCKNCTCEAGKWQCPTEPCSSPSHCADNEFACHLSERCVPNAWVCDNEDDCGDGSDEICLLTCAPHEHRC-ANGQCVPLAHRCDGKADCVDHSDEWSCPAPSCGSNEFRCSNGR-CIPIAHVCDGELDCGFGDDSDESNCSSECSSTHFRCSRGR-CVPYIHHCDGHDDCGDFSDERGCYCDVGEFQCPDGL----------------CIPREKLCDGEKDCPRGTDELLC 1543 HSP 2 Score: 54.299 bits (129), Expect = 2.929e-6 Identity = 36/102 (35.29%), Postives = 52/102 (50.98%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-----------NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG-CL--PHQIVCNISRTC 4697 C+ QF C C++SS +CD DC + DE +C ++C +QF C S G C+ M ++CD ++C D SDE C C+ P + SRTC Sbjct: 2277 CSVGQFACSTFGCVDSSFVCDGQEDCIDGSDEYHCGRFITFLPESASLCSARQFACAS-GECLAMERRCDLHRDCADGSDEDKCADCILSPWTFWSDCSRTC 2377 HSP 3 Score: 53.1434 bits (126), Expect = 6.029e-6 Identity = 45/163 (27.61%), Postives = 65/163 (39.88%), Query Frame = 3 Query: 4443 SQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQN------------------------------------------CEDNSDEISCGC------LPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMT 4787 + FRC G+C+ CD DC + DE+ C CD+ +FQC +G CIP K CDG ++ C D SDE+ C P +C I+R C+ +CDG DC + DE +C++ +T Sbjct: 1476 THFRCSRGRCVPYIHHCDGHDDCGDFSDERGC-YCDVGEFQC-PDGLCIPREKLCDGEKDCPRGTDELLCTGNIGIRAYWVFGYSWTSLLSILLHLCDGEPDCGDGSDELQAQCSTQIPPCPGHFIC-INRMCVNISLLCDGAPDCPQGE--DEVSCERSPVT 1633
BLAST of SCO-spondin vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 370.163 bits (949), Expect = 3.134e-103 Identity = 339/1300 (26.08%), Postives = 552/1300 (42.46%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGC---------PDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVS--SHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP-------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFG--IQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKD--------NCTNP----VCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETAN-CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIII-GGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPY-EISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK----------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGAIIKM-----ENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD 4460 C +G ++ +TFD Y + G CSY+L D + ++S+ Y N + V + + E D+ + G + P + A G ++ F++ +GN+++ + S + GLCGN + +DF + + + F NS+ G P +S+ + + Q K F C LV+ D Y ++C ++C ++ N C Y+ +C+ G W + T+C+ CP +++C S C TC++L + CQ + C C G+V G CI C C++ G+ +PPG+ + DCN+CTC + W C+ + CP C + G + F +FD F F+ + CQY+ ++++T + + ++ + T S + + +T+K+ V +DG+D +P +R T + + V+++ ++ +DG + IK+S + GLCG ++GN +DF I S+ + S+ + G C + + DP ++C +++ F C N +V+ Y CR + S + + CSA+ A AC R G I + P + C P+ + G ++Q C C TCR L+ C + C GC CPP Y G+CV +S+C+C+ P+ + ++ C C NG ++C T D +C P +CP + + +C NY+ + + C CP D + + C+ P CPC + +EYAP + I C +C NR W T N C+A C A G HY T DG + F G C Y L D G + I V N CGFS C K + I+ G+ L + + PL + V++ +F L+ + + V WD+ RVY+ LK +++ V GLCGN+DG N+ + + E D + F S+ N + +SP +C N ++ AE C ++ +FA C + ++ + Y+ C YD C C GDC C C SLA +A CA GI V WR+ +LCP CE Y++C + CP TC P +C CVEGC CP G I +++ C+ P CP C G + +G I + + C+ C C C ITT P+ D Sbjct: 27 LARCSLFGENHIQTFDGTFYDFYGDCSYMLAG--DCNKHSFSLLADYRNGKKKS--VSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEA----GYYKLSSQEHGFMVKIDASGNVQV-----------VLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEELNCP----------CQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSL--NINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIRRDCNSCTCQNGAWECSNEDCPGECFVTGQSHFKSFDNKHFTFSGI-CQYLLATDAMDSTFS-VTIETVQCADDIDAVCTRSASLRLLDMHNITIKLKHGG---------------GVSLDGQD------ILLPLVQGFLRIQTTVLSAVRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTDLMKQDTDPCNLNPKRARFSEEVCFALMDS-AFQSCHN--EVNPAPYLKNCRYDVCSCSNGKECLCSALSTYAAACSRKGILIDWRTP------EFC-PIQCSEGKIYQQCGSPCNQTCRSLSHPDVD-CREFCMEGCYCPPGLYTNEDGECVPKSDCSCYYDGELFQPDDVFSNHYSM----CYCENGLMHCSTSDVPSVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGCISGCTCP-DGMVRHKSKCMFPQRCPCFHAGKEYAPGSSVEIDCNTCVCENRKWKC----TENICDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGT---FRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHIKKPLSDETSFDVLKSGNYFILLLGNQ--MSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSP---------LSLCKDNLMKQMAAENACNIL--MGDLFAECAKVVNPE--PYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQ---HPEPVNCP-LKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQCNACEISVITTVAPTTLPVD 1230 HSP 2 Score: 115.161 bits (287), Expect = 8.023e-25 Identity = 110/403 (27.30%), Postives = 167/403 (41.44%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKF-VQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIAN--ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC---TPSQFRCDDGKCIESSKIC 4493 A C +G+ H +T DG + F G C Y L GD + + + N SL F + + + G ++G + I +E + +L S G V D S V + L GLCGNY+ ++++ M + E++ FA S+ + A C P N S E A D Q C ++K T+ F C + D + Y + C D C C + C C + +A CA G I W T +C +C G Y+ C SPC +TC ++ N C++GCSCP+G + C+P C C ++G+ Y G+ I+ +C C C + C+ + DC C S F+ D K S IC Sbjct: 28 ARCSLFGENHIQTFDGTFYDFYGDCSYMLA---GDCNKHSFSLLADYRNGKKKSVSLYLGEYFDIHLFLDGTA---TQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSE----TAEKDFM----QRCQMLK-TSSAFMQCHHLV--DPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSLNIN---EVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIR-RDCNSCTCQNGAWECSNE--DCPGECFVTGQSHFKSFDNKHFTFSGIC 405 HSP 3 Score: 70.8626 bits (172), Expect = 2.377e-11 Identity = 92/376 (24.47%), Postives = 144/376 (38.30%), Query Frame = 3 Query: 3243 KCAKYICSNRMWNLLSEETANCEA----VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLSRG-----NNDTIPLFPKYDIVIEQLTFFTQLVSD----SIGLRVLWDRSTRVYLF-LKPS-FQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRK--SWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC-DTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 K K +C N + + EET C +C+ H T DG F G+C Y L + + + + + N C + TC K V++ + LLS N D +PL D ++T + ++V + +G + + ++ L P+ F GLCG D + N++ T+ + FI E V S + C PD+ C +M + EA H Y++ C +C G D +C +A + C G+ + WRT + CP+ C Y C C + C T+ S ST EGC CP G + +NG+CV C Sbjct: 1926 KIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSYVLF------HDEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSNMQVSVNGDIVPLPYANDNF--EVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDF---TLSDGSVTTDSSKFIK-----------EWTVVDSLGR-TCETKPDKACTQPISSECKIM------LSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLNGTLCSDSST----EGCFCPEGDVIVNGKCVNEKVC 2262 HSP 4 Score: 53.1434 bits (126), Expect = 6.246e-6 Identity = 87/389 (22.37%), Postives = 141/389 (36.25%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLR--QDNFILLSLEAGN-IKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNS--FKGFGCPDIANTLSTCKPDD---QILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYD--GQEH---PPGTVRQFDCNTCTC-NSSLWHCTEKICPS----TCG 1883 C + TFD + G CSY+L +V V ++ N T+ C+K + ++ + S N N + PL N + + + G ++ ++ Q F+ A N L+ + +GLCG D N NDF+ D + +S K + D KPD Q ++++ + F C + YF++C + C +C ++AY C G CI WR+ C +CP ++ C C C C +S C C G+V C+ + C D G+EH C+ C C + + +CT CP+ TCG Sbjct: 1955 CMGSSTRHIVTFDGLDFKLMGACSYVLFH-DEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSN---MQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLR----------TSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASCHGQD--------------ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLN--GTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCG 2313
BLAST of SCO-spondin vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 368.237 bits (944), Expect = 1.619e-102 Identity = 338/1311 (25.78%), Postives = 553/1311 (42.18%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG----CPDIANTLSTCKPDDQILANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVS--SHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP-------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFG--IQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCT---------------------------NPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETAN-CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIII-GGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK----------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGAIIKM-----ENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD 4460 C +G ++ +TFD Y + G CSY+L + S +++ + V + + E D+ + G + P + A G ++ F++ +GN+++ + S + GLCGN + +DF + + + F NS+ G C +TC + Q+C ++ F C LV+ D Y ++C ++C ++ N C Y+ +C+ G W + T+C+ CP +++C S C TC++L + CQ + C C G+V G CI C C++ G+ +PPG+ + DCN+CTC + W C+ + CP C + G + F +FD F F+ + CQY+ ++++T + + ++ + T S + + +T+K+ V +DG+D +P +R T + + V+++ ++ +DG + IK+S + GLCG ++GN +DF I S+ + S+ + G C + + DP ++C +++ F C N +V+ Y CR + S + + CSA+ A AC R G I + P + C P+ + G ++Q C C TCR L+ C + C GC CPP Y G+CV +S+C+C+ D E+ ++ N + C NG ++C T D+C +CP + + +C NY+ + + C CP D + + C+ P CPC + +EYAP + I C +C NR W T N C+A C A G HY T DG + F G C Y L D G + I V N CGFS C K + I+ G+ L + + PL + V++ +F L+ + + V WD+ RVY+ LK +++ V GLCGN+DG N+ + + E D + F S+ N +C +SP + +C N ++ AE C ++ +FA C + ++ + Y+ C YD C C GDC C C SLA +A CA GI V WR+ +LCP CE Y++C + CP TC P +C CVEGC CP G I +++ C+ P CP C G + +G I + + C+ C C C ITT P+ D Sbjct: 23 LARCSLFGENHIQTFDGTFYDFYGDCSYMLAGDCN----KHSFSLLGKVVNGKKKSVSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEA----GYYKLSSQEHGFMVKIDASGNVQV-----------VLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEELNCP----------CQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSL--NINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIRRDCNSCTCQNGAWECSNEDCPGECFVTGQSHFKSFDNKHFTFSGI-CQYLLATDAMDSTFS-VTIETVQCADDIDAVCTRSASLRLLDMHNITIKLKHGG---------------GVSLDGQD------ILLPLVQGFLRIQTTVLSAVRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTDLMKQDTDPCNLNPKRARFSEEVCFALMDS-AFQSCHN--EVNPAPYLKNCRYDVCSCSNGKECLCSALSTYAAACSRKGILIDWRTP------EFC-PIQCSEGKIYQQCGSPCNQTCRSLSHPDVD-CREFCMEGCYCPPGLYTNEDGECVPKSDCSCY-----YDGELFQPDDVFSNHYSIYCENGLMHCSTSDSCVLSIVLSYMYIFAFNTVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGCISGCTCP-DGMVRHKSKCMFPQRCPCFHAGKEYAPGSSVEIDCNTCVCENRKWKC----TENICDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGT---FRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHIKKPLSDETSFDVLKSGNYFILLLGNQ--MSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNP-LCFGAVSPLS---------LCKDNLMKQMAAENACNIL--MGDLFAECAKVVNPE--PYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQ---HPEPVNCP-LKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQCNACEISVITTVAPTTLPVD 1235 HSP 2 Score: 122.479 bits (306), Expect = 5.459e-27 Identity = 113/421 (26.84%), Postives = 176/421 (41.81%), Query Frame = 3 Query: 3270 RMWNLLSEETANCE----AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKF-VQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIAN--ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC---TPSQFRCDDGKCIESSKIC 4493 ++W L+++ T + A C +G+ H +T DG + F G C Y L GD + + + V N SL F + + + G ++G + I +E + +L S G V D S V + L GLCGNY+ ++++ M + E++ FA S+ + A C P N S E D Q C ++K T+ F C + D + Y + C D C C + C C + +A CA G I W T +C +C G Y+ C SPC +TC ++ N C++GCSCP+G + C+P C C ++G+ Y G+ I+ +C C C + C+ + DC C S F+ D K S IC Sbjct: 6 QIWMLVAQITGKLDWSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLA---GDCNKHSFSLLGKVVNGKKKSVSLYLGEYFDIHLFLDGTA---TQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETDF-----------MQRCQMLK-TSSAFMQCHHLV--DPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSLNIN---EVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIR-RDCNSCTCQNGAWECSNE--DCPGECFVTGQSHFKSFDNKHFTFSGIC 398 HSP 3 Score: 68.1662 bits (165), Expect = 1.535e-10 Identity = 90/374 (24.06%), Postives = 146/374 (39.04%), Query Frame = 3 Query: 3243 KCAKYICSNRMWNLLSEETANCEA----VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLS---RGNNDTIPLFPKYDIVIEQLTFFTQLVSD----SIGLRVLWDRSTRVYLF-LKPS-FQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRK--SWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC-DTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 K K +C N + + EET C +C+ H T DG F G+C Y L + + + + + N C + TC K V++ + LLS + +++ +PL D ++T + ++V + +G + + ++ L P+ F GLCG D + N++ T+ + FI E V S + C PD+ C +M + EA H Y++ C +C G D +C +A + C G+ + WRT + CP+ C Y C C + C T+ S ST EGC CP G + +NG+CV C Sbjct: 1888 KIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSYVLF------HDEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSNMQVSDNIVPLPYANDNF--EVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDF---TLSDGSVTTDSSKFIK-----------EWTVVDSLGR-TCETKPDKACTQPISSECKIM------LSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLNGTLCSDSST----EGCFCPEGDVIVNGKCVNEKVC 2222 HSP 4 Score: 55.4546 bits (132), Expect = 1.160e-6 Identity = 84/380 (22.11%), Postives = 141/380 (37.11%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLR--QDNFILLSLEAGN-IKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNS--FKGFGCPDIANTLSTCKPDD---QILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYD--GQEHPPGTVRQFDCNTCTC-NSSLWHCTEKICPST 1877 C + TFD + G CSY+L +V V ++ N T+ C+K + ++ + S N + +D+ + PL N + + + G ++ ++ Q F+ A N L+ + +GLCG D N NDF+ D + +S K + D KPD Q ++++ + F C + YF++C + C +C ++AY C G CI WR+ C +CP ++ C C C C +S C C G+V C+ + C D G+EH C+ C C + + +CT CP+ Sbjct: 1917 CMGSSTRHIVTFDGLDFKLMGACSYVLFH-DEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSN--MQVSDN---IVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLR----------TSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASCHGQD--------------ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLN--GTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQTWIPTNAPCSICMCLENRMINCTAMPCPTV 2264
BLAST of SCO-spondin vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 365.155 bits (936), Expect = 1.542e-101 Identity = 341/1317 (25.89%), Postives = 555/1317 (42.14%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGC---------PDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVS--SHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP-------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFG--IQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKD----------------------NCTNP----VCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETAN-CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIII-GGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIAN---ADICPY-EISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK----------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGAIIKM-----ENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD 4460 C +G ++ +TFD Y + G CSY+L D + ++S+ Y N + V + + E D+ + G + P + A G ++ F++ +GN+++ + S + GLCGN + +DF + + + F NS+ G P +S+ + + Q K F C LV+ D Y ++C ++C ++ N C Y+ +C+ G W + T+C+ CP +++C S C TC++L + CQ + C C G+V G CI C C++ G+ +PPG+ + DCN+CTC + W C+ + CP C + G + F +FD F F+ + CQY+ ++++T + + ++ + T S + + +T+K+ V +DG+D +P +R T + + V+++ ++ +DG + IK+S + GLCG ++GN +DF I S+ + S+ + G C + + DP ++C +++ F C N +V+ Y CR + S + + CSA+ A AC R G I + P + C P+ + G ++Q C C TCR L+ C + C GC CPP Y G+CV +S+C+C+ P+ + ++ C C NG ++C T D +C P +CP + + +C NY+ + + C CP D + + C+ P CPC + +EYAP + I C +C NR W T N C+A C A G HY T DG + F G C Y L D G + I V N CGFS C K + I+ G+ L + + PL + V++ +F L+ + + V WD+ RVY+ LK +++ V GLCGN+DG N+ + + E D + F S+ N AD + +SP + +C N ++ AE C ++ +FA C + ++ + Y+ C YD C C GDC C C SLA +A CA GI V WR+ +LCP CE Y++C + CP TC P +C CVEGC CP G I +++ C+ P CP C G + +G I + + C+ C C C ITT P+ D Sbjct: 38 LARCSLFGENHIQTFDGTFYDFYGDCSYMLAG--DCNKHSFSLLADYRNGKKKS--VSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEA----GYYKLSSQEHGFMVKIDASGNVQV-----------VLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEELNCP----------CQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSL--NINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIRRDCNSCTCQNGAWECSNEDCPGECFVTGQSHFKSFDNKHFTFSGI-CQYLLATDAMDSTFS-VTIETVQCADDIDAVCTRSASLRLLDMHNITIKLKHGG---------------GVSLDGQD------ILLPLVQGFLRIQTTVLSAVRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTDLMKQDTDPCNLNPKRARFSEEVCFALMDS-AFQSCHN--EVNPAPYLKNCRYDVCSCSNGKECLCSALSTYAAACSRKGILIDWRTP------EFC-PIQCSEGKIYQQCGSPCNQTCRSLSHPDVD-CREFCMEGCYCPPGLYTNEDGECVPKSDCSCYYDGELFQPDDVFSNHYSM----CYCENGLMHCSTSDVPSGYFPESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGCISGCTCP-DGMVRHKSKCMFPQRCPCFHAGKEYAPGSSVEIDCNTCVCENRKWKC----TENICDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGT---FRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHIKKPLSDETSFDVLKSGNYFILLLGNQ--MSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADAAKFGAVSPLS---------LCKDNLMKQMAAENACNIL--MGDLFAECAKVVNPE--PYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQ---HPEPVNCP-LKCVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQCNACEISVITTVAPTTLPVD 1258 HSP 2 Score: 115.931 bits (289), Expect = 5.670e-25 Identity = 115/430 (26.74%), Postives = 174/430 (40.47%), Query Frame = 3 Query: 3255 YICSNRMWNLLSEETANCE--------AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKF-VQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIAN--ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC---TPSQFRCDDGKCIESSKIC 4493 Y+ + W L T E A C +G+ H +T DG + F G C Y L GD + + + N SL F + + + G ++G + I +E + +L S G V D S V + L GLCGNY+ ++++ M + E++ FA S+ + A C P N S E A D Q C ++K T+ F C + D + Y + C D C C + C C + +A CA G I W T +C +C G Y+ C SPC +TC ++ N C++GCSCP+G + C+P C C ++G+ Y G+ I+ +C C C + C+ + DC C S F+ D K S IC Sbjct: 12 YLLTVLRWIFLLRTTLFWEGKLDWSVLARCSLFGENHIQTFDGTFYDFYGDCSYMLA---GDCNKHSFSLLADYRNGKKKSVSLYLGEYFDIHLFLDGTA---TQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSE----TAEKDFM----QRCQMLK-TSSAFMQCHHLV--DPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSLNIN---EVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIR-RDCNSCTCQNGAWECSNE--DCPGECFVTGQSHFKSFDNKHFTFSGIC 416 HSP 3 Score: 70.4774 bits (171), Expect = 3.556e-11 Identity = 92/376 (24.47%), Postives = 144/376 (38.30%), Query Frame = 3 Query: 3243 KCAKYICSNRMWNLLSEETANCEA----VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLSRG-----NNDTIPLFPKYDIVIEQLTFFTQLVSD----SIGLRVLWDRSTRVYLF-LKPS-FQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRK--SWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC-DTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 K K +C N + + EET C +C+ H T DG F G+C Y L + + + + + N C + TC K V++ + LLS N D +PL D ++T + ++V + +G + + ++ L P+ F GLCG D + N++ T+ + FI E V S + C PD+ C +M + EA H Y++ C +C G D +C +A + C G+ + WRT + CP+ C Y C C + C T+ S ST EGC CP G + +NG+CV C Sbjct: 1919 KIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSYVLF------HDEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSNMQVSVNGDIVPLPYANDNF--EVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDF---TLSDGSVTTDSSKFIK-----------EWTVVDSLGR-TCETKPDKACTQPISSECKIM------LSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLNGTLCSDSST----EGCFCPEGDVIVNGKCVNEKVC 2255 HSP 4 Score: 52.7582 bits (125), Expect = 8.377e-6 Identity = 87/389 (22.37%), Postives = 141/389 (36.25%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLR--QDNFILLSLEAGN-IKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNS--FKGFGCPDIANTLSTCKPDD---QILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYD--GQEH---PPGTVRQFDCNTCTC-NSSLWHCTEKICPS----TCG 1883 C + TFD + G CSY+L +V V ++ N T+ C+K + ++ + S N N + PL N + + + G ++ ++ Q F+ A N L+ + +GLCG D N NDF+ D + +S K + D KPD Q ++++ + F C + YF++C + C +C ++AY C G CI WR+ C +CP ++ C C C C +S C C G+V C+ + C D G+EH C+ C C + + +CT CP+ TCG Sbjct: 1948 CMGSSTRHIVTFDGLDFKLMGACSYVLFH-DEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSN---MQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLR----------TSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASCHGQD--------------ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLN--GTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCG 2306
BLAST of SCO-spondin vs. Ensembl Xenopus
Match: ndufs8 (NADH:ubiquinone oxidoreductase core subunit S8 [Source:Xenbase;Acc:XB-GENE-945877]) HSP 1 Score: 364.385 bits (934), Expect = 2.411e-101 Identity = 339/1314 (25.80%), Postives = 553/1314 (42.09%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGC---------PDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVS--SHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP-------PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFG--IQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKD----------------------NCTNP----VCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETAN-CEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIII-GGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPY-EISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK----------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGAIIKM-----ENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD 4460 C +G ++ +TFD Y + G CSY+L D + ++S+ Y N + V + + E D+ + G + P + A G ++ F++ +GN+++ + S + GLCGN + +DF + + + F NS+ G P +S+ + + Q K F C LV+ D Y ++C ++C ++ N C Y+ +C+ G W + T+C+ CP +++C S C TC++L + CQ + C C G+V G CI C C++ G+ +PPG+ + DCN+CTC + W C+ + CP C + G + F +FD F F+ + CQY+ ++++T + + ++ + T S + + +T+K+ V +DG+D +P +R T + + V+++ ++ +DG + IK+S + GLCG ++GN +DF I S+ + S+ + G C + + DP ++C +++ F C N +V+ Y CR + S + + CSA+ A AC R G I + P + C P+ + G ++Q C C TCR L+ C + C GC CPP Y G+CV +S+C+C+ P+ + ++ C C NG ++C T D +C P +CP + + +C NY+ + + C CP D + + C+ P CPC + +EYAP + I C +C NR W T N C+A C A G HY T DG + F G C Y L D G + I V N CGFS C K + I+ G+ L + + PL + V++ +F L+ + + V WD+ RVY+ LK +++ V GLCGN+DG N+ + + E D + F S+ N + +SP + +C N ++ AE C ++ +FA C + ++ + Y+ C YD C C GDC C C SLA +A CA GI V WR+ +LCP CE Y++C + CP TC P +C CVEGC CP G I +++ C+ P CP C G + +G I + + C+ C C C ITT P+ D Sbjct: 27 LARCSLFGENHIQTFDGTFYDFYGDCSYMLAG--DCNKHSFSLLADYRNGKKKS--VSLYLGEYFDIHLFLDGTATQGEKHISMPYASNGIFLESEA----GYYKLSSQEHGFMVKIDASGNVQV-----------VLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSETAEKDFMQRCQMLKTSSAFMQCHHLVDPDPYIAICEDDMCECAEELNCP----------CQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSL--NINEVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIRRDCNSCTCQNGAWECSNEDCPGECFVTGQSHFKSFDNKHFTFSGI-CQYLLATDAMDSTFS-VTIETVQCADDIDAVCTRSASLRLLDMHNITIKLKHGG---------------GVSLDGQD------ILLPLVQGFLRIQTTVLSAVRLTYKEDLQVDWDGHGKLLIKLSPIYSGLISGLCGNYNGNQGDDFLTPSGLIETSVEDFGNSWKLNGDCTDLMKQDTDPCNLNPKRARFSEEVCFALMDS-AFQSCHN--EVNPAPYLKNCRYDVCSCSNGKECLCSALSTYAAACSRKGILIDWRTP------EFC-PIQCSEGKIYQQCGSPCNQTCRSLSHPDVD-CREFCMEGCYCPPGLYTNEDGECVPKSDCSCYYDGELFQPDDVFSNHYSM----CYCENGLMHCSTSDVPSGYFPESFFNQPPARVKRSLDCRPPLNKIICPADNPKATGIECAKTCQNYELECVSHGCISGCTCP-DGMVRHKSKCMFPQRCPCFHAGKEYAPGSSVEIDCNTCVCENRKWKC----TENICDATCSAIGVSHYLTFDGFRYKFPGDCQYVLAQDYCGGVTGT---FRILVGNEGCGFSGEKCTKRITILFRNGEIELTNEQIHIKKPLSDETSFDVLKSGNYFILLLGNQ--MSVTWDKGMRVYVTLKETYRDKVCGLCGNFDGIENNDLISSNNQIEMDPVNFGNSWKVNPLCADFGAVSPLS---------LCKDNLMKQMAAENACNIL--MGDLFAECAKVVNPE--PYWEICTYDTCTCESAGDCACFCDSLAAYAHVCAQRGINVHWRSSHLCPQSCEEKNQRDLDYVCEWRYNSCTTACPVTCQ---HPEPVNCPLK-CVEGCQAYCPPGKILDEVSESCIEPKDCPVCTIEGHTVPHGRKIILNHDDPQRCQLCHCEGKNLQCNACEISVITTVAPTTLPVD 1244 HSP 2 Score: 115.161 bits (287), Expect = 9.384e-25 Identity = 110/403 (27.30%), Postives = 167/403 (41.44%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKF-VQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIAN--ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC---TPSQFRCDDGKCIESSKIC 4493 A C +G+ H +T DG + F G C Y L GD + + + N SL F + + + G ++G + I +E + +L S G V D S V + L GLCGNY+ ++++ M + E++ FA S+ + A C P N S E A D Q C ++K T+ F C + D + Y + C D C C + C C + +A CA G I W T +C +C G Y+ C SPC +TC ++ N C++GCSCP+G + C+P C C ++G+ Y G+ I+ +C C C + C+ + DC C S F+ D K S IC Sbjct: 28 ARCSLFGENHIQTFDGTFYDFYGDCSYMLA---GDCNKHSFSLLADYRNGKKKSVSLYLGEYFDIHLFLDGTA---TQGEKHISMPYASNGIFLESEAGYYKLSSQEHGFMVKIDASGNVQVVLPSKHFNKTCGLCGNYNQFAEDDFMMQEGILTENSYDFANSWALHGGARRCRRTYPPSNTCNISSE----TAEKDFM----QRCQMLK-TSSAFMQCHHLV--DPDPYIAICEDDMCEC--AEELNCPCQTFLEYARTCAQHGVITSNWPTHTICKPLCPFGMEYNECVSPCVKTCQSLNIN---EVCQEQCIDGCSCPDGKVLDGDRCIPVFECSCIHSGKRYPPGSAIR-RDCNSCTCQNGAWECSNE--DCPGECFVTGQSHFKSFDNKHFTFSGIC 405 HSP 3 Score: 70.4774 bits (171), Expect = 3.028e-11 Identity = 92/376 (24.47%), Postives = 144/376 (38.30%), Query Frame = 3 Query: 3243 KCAKYICSNRMWNLLSEETANCEA----VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS-LATCAKFVQIIIGGQTYLLSRG-----NNDTIPLFPKYDIVIEQLTFFTQLVSD----SIGLRVLWDRSTRVYLF-LKPS-FQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRK--SWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC-DTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTC 4307 K K +C N + + EET C +C+ H T DG F G+C Y L + + + + + N C + TC K V++ + LLS N D +PL D ++T + ++V + +G + + ++ L P+ F GLCG D + N++ T+ + FI E V S + C PD+ C +M + EA H Y++ C +C G D +C +A + C G+ + WRT + CP+ C Y C C + C T+ S ST EGC CP G + +NG+CV C Sbjct: 1903 KIPKPMCHNNLPAMKIEETCGCRWTCPCMCMGSSTRHIVTFDGLDFKLMGACSYVLF------HDEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSNMQVSVNGDIVPLPYANDNF--EVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLRTSGLCGICDQNTVNDF---TLSDGSVTTDSSKFIK-----------EWTVVDSLGR-TCETKPDKACTQPISSECKIM------LSKTFEACHTIFPPKPYFTVCEEASC---HGQD---ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLNGTLCSDSST----EGCFCPEGDVIVNGKCVNEKVC 2239 HSP 4 Score: 52.7582 bits (125), Expect = 7.137e-6 Identity = 87/389 (22.37%), Postives = 141/389 (36.25%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLR--QDNFILLSLEAGN-IKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNS--FKGFGCPDIANTLSTCKPDD---QILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYD--GQEH---PPGTVRQFDCNTCTC-NSSLWHCTEKICPS----TCG 1883 C + TFD + G CSY+L +V V ++ N T+ C+K + ++ + S N N + PL N + + + G ++ ++ Q F+ A N L+ + +GLCG D N NDF+ D + +S K + D KPD Q ++++ + F C + YF++C + C +C ++AY C G CI WR+ C +CP ++ C C C C +S C C G+V C+ + C D G+EH C+ C C + + +CT CP+ TCG Sbjct: 1932 CMGSSTRHIVTFDGLDFKLMGACSYVLFH-DEVHDVKVILHNAECNTTNHQTCMKSVEVKHNKYSILLSSN---MQVSVNGDIVPLPYANDNFEVTLYGEIVHEVKIPQLGFVFTFTPANNEFVLQLNPAAFSLR----------TSGLCGICDQNTVNDFTLSDGSVTTDSSKFIKEWTVVDSLGRTCETKPDKACTQPISSECKIMLSKTFEACHTIFPPKPYFTVCEEASCHGQD--------------ICEVIAAYVHVCRINGVCINWRTPDFCPMECPRLMVYHHCHMGCNKECGKTLN--GTLCSDSSTEGCFCPEGDVIVNGKCVNEKVCSQCTDRYGEEHQLLETWIPTNAPCSICMCLENRMINCTAMPCPTVKPLTCG 2290
BLAST of SCO-spondin vs. Ensembl Mouse
Match: Sspo (SCO-spondin [Source:MGI Symbol;Acc:MGI:2674311]) HSP 1 Score: 469.159 bits (1206), Expect = 7.486e-135 Identity = 403/1462 (27.56%), Postives = 601/1462 (41.11%), Query Frame = 3 Query: 660 ECCADKTGS--WGSQSGSCVSCKANFAGGN------------------DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYE-NCTSDTGCVKVIVIEAGDVVVKSSG----NGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXX-----XXXXXXXXVASFGNSFK--------------GFGCPDI-ANTLSTCKPDD-QILANQQCSKI-RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDV-GCQYVAVEPSVNNTDDRANVFVIIQS---NTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSC---DNTPQ--SIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITIN------CQTCVCI-NGRLNCKTKDNCTN--PVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVL----------WDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC---ITTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 ECCA G W S S C+SC GN TC ++ +Y+TFD Y + G+C+Y+L D TW++++ +C C V V+ + V+ G G L +S+ L++ G V+R D +S+ + + G GLCG + P A+FGNS+K +GC + TL+ + Q A C ++ F C V D Y C+ C + N + +C + Y+ +C+ + + WR CE CPG +++SDC S CP +C + Q C V C C +G G C+ CPC + Q + PG + CN C C WHC + +CP+ C + G + TFDG F F GC Y V+ SV + + V+++ +T S L ++V + + I L+ + V +DGED LP+ I + ++ ++ + + + L+ + YI + + GLCG F ++DF I S+ A + G C D +P S + IL+G F C VD+E + C + +D L C + C + G+ + + + L P+ GG V+Q C+ C C E + K C GC CPP GQCV S C C G TIN C C+C G NC T +C +CP+ +Y+ + C+S +C S+ + C CP TV +D CV P CPC + + Y PN I C +C + W+ + C C A G PHY T DG +F+F G+C Y L+ G ++SV N+ CG S TC K + + + + RG T+ I + ++ L GL +L WD TRV + L P F G V GLCGN+D D N+ E + +CP P C N R +WA C V+ +FA C H +V +YY C+YDACGC+ GGDCECLC+++AT+A +CA V+WR+Q LCP+ CEGG+ Y+ C S CP TC S HC CVEGC CP G + G C+ CPC + G + G +++ ++C C C ++++C + C C + C ++G C+ +CD DC + DE+ C +VC Q C S G C+P++ CDG +C D +DE C C + C R C+ +C+G+ DC D +DEE+C Sbjct: 138 ECCAQPWGHSWWNSSSQMCLSCSGQHRPGNASSEGLLQPLAGAVGQLWSQRQRPSATCATWSGFHYQTFDGHHYHFLGQCTYLLAGAMDS---TWAVHLRPSVHCPQHRHCWLVQVVMGPEEVLIQDGEVSVKGQPVPVGEPQLLHGMSLQWQGDWLVLSGGLGVVVRLDRSSSISISVDH------------------EFWGRTQGLCGLYNGRPEDDFVEPGGGLATLAATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGPFWQCHGQVQPDEYHETCLFAYCVGATAGNGPE---GQLEAVCATFANYAQACARQHIYVHWRKPGFCERVCPGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGALCVPAAHCPCYHRRQRYAPGDTVKQQCNPCVCQDGRWHCAQALCPAECAVGGDGHYFTFDGRSFFFRGTPGCHYSLVQDSV-----KGQLLVVLEHGACDTGSCL------------HALSVFLGNTHIQLRYSG--------AVLVDGED--VDLPW-IGVEGFNISWASSTFLLLHWPGAWVLWGVAEPAAYITLDPRHAYQVQGLCGTFTWKQQDDFLTPAGDIETSVTAFASKFQVSGDGRCPLVDKSPLFCSSYSQHLTFTEAACAILHGHAFQECHG--LVDREPFRLRCLEAVCGCAPGRDCL--CPVLSAYTRHCAQEGVLLQ--WRNETL---CPVSCPGGQVYQECAPVCGHHCGEPEDC---KELGICVAGCNCPPGLLWDLEGQCVPPSMCHCQF----------GGHRYTINTTTVRDCSHCICQERGLWNC-TAHHCPRQWALCPRELIYVPGACLLTCDSPRANHSCWAGST--DGCVCPPGTV-LLDKHCVSPDLCPCRHNGQWYPPNATIQEDCNICVCQGQRWHCTGQ---RCSGWCQASGAPHYVTFDGLVFTFPGACEYLLVREAGGR------FSVSVQNLPCGASGLTCTKALVVRLDSTVVHMLRGQAVTVN---GVSIRLPKVYTGPGLSLHHAGLFLLLTTRLGLTLLWDGGTRVLVQLSPHFHGRVAGLCGNFDSDASNDLRSRQGVLEPTAELTAHSWRLNPLCP---------EPGDLPHPCTVNAHRANWARARCEVILQP--IFAPC----HTEVPPQQYYEWCVYDACGCDTGGDCECLCSAIATYADECARHRHHVRWRSQELCPLQCEGGQVYEPCGSTCPPTCHDHHSELRWHCQVITCVEGCFCPEGTLLHGGACMKLAACPCEWQGSFFPPGTVLQ-KDCGNCTCQGSQWHCDRGGAPCEDMEPGCAEGETLCRENGHCVPLEWLCDNQDDCGDGSDEEGCATSVCGEGQMSCQS-GHCLPLSLICDGQDDCGDGTDEQGCLCPHGSLACADGR-CLPPALLCNGHPDC--LDAADEESC 1489 HSP 2 Score: 65.4698 bits (158), Expect = 9.099e-10 Identity = 58/215 (26.98%), Postives = 89/215 (41.40%), Query Frame = 3 Query: 4242 EGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCI--TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDE--KNCNV----------------------------------CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG--CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEE 4763 +GC CP+G + +G C+PP NG C+ A D C+ +C + C DG C+ + ++CD V DC + DE +C++ C L +FQC ++G C P +CD ++C D SDE+ CG C+ +Q+ C S C+ ++CDG C D SDE+ Sbjct: 1451 QGCLCPHGSLACADGRCLPPAL---LCNGHP---------------DCLDAA-----DEESCLGPVSCISGEVSCVDGTCVRTIQLCDGVWDCPDGADEGPSHCSLPSLPTPPGGIGQNPSTSSLDTAPSPVGSTSPASPCSLLEFQC-NSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQC--PDGSDED 1639 HSP 3 Score: 62.003 bits (149), Expect = 1.083e-8 Identity = 42/107 (39.25%), Postives = 48/107 (44.86%), Query Frame = 3 Query: 1581 CETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCG 1883 C QCPGN ++ C E CP C L Q P C S C C G + +C+ CPC GQ + PG V DCN CTC S CT K CP CG Sbjct: 2852 CTAQCPGNMVFRSAEQCLEEGGPCPQLC--LAQDPGVECTGSCAPSCNCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVAHLDCNNCTCISGEMVCTSKRCPVACG 2956 HSP 4 Score: 56.9954 bits (136), Expect = 3.114e-7 Identity = 40/132 (30.30%), Postives = 60/132 (45.45%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISR-------------TCIEEKKICDGNYDCGVNDKS----DEENC 4769 + C+ +F+C+ G+C CD+ DC++ DE +C C L Q C + C+ + CDG C D SDE C +R TCI+ +CDGN DC + D++ DE+ C Sbjct: 1566 SPCSLLEFQCNSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQCPDGSDEDPDVCEEQSASGGANRTGAPCPEFSCPDGTCIDFLLVCDGNPDCELADETEPSLDEQGC 1697 HSP 5 Score: 54.6842 bits (130), Expect = 1.800e-6 Identity = 38/123 (30.89%), Postives = 55/123 (44.72%), Query Frame = 3 Query: 2766 CQSTCRELNEATA-SKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSC 3131 C+ TC E+N A S C + PGC C Y+++ TG CV E C CW+ P P G+ C++C C++G+ C +C C + +V M C DT + SC Sbjct: 4925 CEKTCPEMNMTQAWSNCTEAQAPGCVCQLGYFRSQTGLCVPEDHCECWHHGSPHLP----GSEWQEACESCRCLHGKSVCIR--HCPELSCAQGEVIMQEPGSCCPICQQDTLSTEEEEPVSC 5041
BLAST of SCO-spondin vs. Ensembl Mouse
Match: Sspo (SCO-spondin [Source:MGI Symbol;Acc:MGI:2674311]) HSP 1 Score: 468.389 bits (1204), Expect = 1.105e-134 Identity = 403/1462 (27.56%), Postives = 601/1462 (41.11%), Query Frame = 3 Query: 660 ECCADKTGS--WGSQSGSCVSCKANFAGGN------------------DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYE-NCTSDTGCVKVIVIEAGDVVVKSSG----NGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXX-----XXXXXXXXVASFGNSFK--------------GFGCPDI-ANTLSTCKPDD-QILANQQCSKI-RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDV-GCQYVAVEPSVNNTDDRANVFVIIQS---NTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSC---DNTPQ--SIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITIN------CQTCVCI-NGRLNCKTKDNCTN--PVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVL----------WDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC---ITTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 ECCA G W S S C+SC GN TC ++ +Y+TFD Y + G+C+Y+L D TW++++ +C C V V+ + V+ G G L +S+ L++ G V+R D +S+ + + G GLCG + P A+FGNS+K +GC + TL+ + Q A C ++ F C V D Y C+ C + N + +C + Y+ +C+ + + WR CE CPG +++SDC S CP +C + Q C V C C +G G C+ CPC + Q + PG + CN C C WHC + +CP+ C + G + TFDG F F GC Y V+ SV + + V+++ +T S L ++V + + I L+ + V +DGED LP+ I + ++ ++ + + + L+ + YI + + GLCG F ++DF I S+ A + G C D +P S + IL+G F C VD+E + C + +D L C + C + G+ + + + L P+ GG V+Q C+ C C E + K C GC CPP GQCV S C C G TIN C C+C G NC T +C +CP+ +Y+ + C+S +C S+ + C CP TV +D CV P CPC + + Y PN I C +C + W+ + C C A G PHY T DG +F+F G+C Y L+ G ++SV N+ CG S TC K + + + + RG T+ I + ++ L GL +L WD TRV + L P F G V GLCGN+D D N+ E + +CP P C N R +WA C V+ +FA C H +V +YY C+YDACGC+ GGDCECLC+++AT+A +CA V+WR+Q LCP+ CEGG+ Y+ C S CP TC S HC CVEGC CP G + G C+ CPC + G + G +++ ++C C C ++++C + C C + C ++G C+ +CD DC + DE+ C +VC Q C S G C+P++ CDG +C D +DE C C + C R C+ +C+G+ DC D +DEE+C Sbjct: 138 ECCAQPWGHSWWNSSSQMCLSCSGQHRPGNASSEGLLQPLAGAVGQLWSQRQRPSATCATWSGFHYQTFDGHHYHFLGQCTYLLAGAMDS---TWAVHLRPSVHCPQHRHCWLVQVVMGPEEVLIQDGEVSVKGQPVPVGEPQLLHGMSLQWQGDWLVLSGGLGVVVRLDRSSSISISVDH------------------EFWGRTQGLCGLYNGRPEDDFVEPGGGLATLAATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGPFWQCHGQVQPDEYHETCLFAYCVGATAGNGPE---GQLEAVCATFANYAQACARQHIYVHWRKPGFCERVCPGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGALCVPAAHCPCYHRRQRYAPGDTVKQQCNPCVCQDGRWHCAQALCPAECAVGGDGHYFTFDGRSFFFRGTPGCHYSLVQDSV-----KGQLLVVLEHGACDTGSCL------------HALSVFLGNTHIQLRYSG--------AVLVDGED--VDLPW-IGVEGFNISWASSTFLLLHWPGAWVLWGVAEPAAYITLDPRHAYQVQGLCGTFTWKQQDDFLTPAGDIETSVTAFASKFQVSGDGRCPLVDKSPLFCSSYSQHLTFTEAACAILHGHAFQECHG--LVDREPFRLRCLEAVCGCAPGRDCL--CPVLSAYTRHCAQEGVLLQ--WRNETL---CPVSCPGGQVYQECAPVCGHHCGEPEDC---KELGICVAGCNCPPGLLWDLEGQCVPPSMCHCQF----------GGHRYTINTTTVRDCSHCICQERGLWNC-TAHHCPRQWALCPRELIYVPGACLLTCDSPRANHSCWAGST--DGCVCPPGTV-LLDKHCVSPDLCPCRHNGQWYPPNATIQEDCNICVCQGQRWHCTGQ---RCSGWCQASGAPHYVTFDGLVFTFPGACEYLLVREAGGR------FSVSVQNLPCGASGLTCTKALVVRLDSTVVHMLRGQAVTVN---GVSIRLPKVYTGPGLSLHHAGLFLLLTTRLGLTLLWDGGTRVLVQLSPHFHGRVAGLCGNFDSDASNDLRSRQGVLEPTAELTAHSWRLNPLCP---------EPGDLPHPCTVNAHRANWARARCEVILQP--IFAPC----HTEVPPQQYYEWCVYDACGCDTGGDCECLCSAIATYADECARHRHHVRWRSQELCPLQCEGGQVYEPCGSTCPPTCHDHHSELRWHCQVITCVEGCFCPEGTLLHGGACMKLAACPCEWQGSFFPPGTVLQ-KDCGNCTCQGSQWHCDRGGAPCEDMEPGCAEGETLCRENGHCVPLEWLCDNQDDCGDGSDEEGCATSVCGEGQMSCQS-GHCLPLSLICDGQDDCGDGTDEQGCLCPHGSLACADGR-CLPPALLCNGHPDC--LDAADEESC 1489 HSP 2 Score: 65.4698 bits (158), Expect = 9.651e-10 Identity = 58/215 (26.98%), Postives = 89/215 (41.40%), Query Frame = 3 Query: 4242 EGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCI--TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDE--KNCNV----------------------------------CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG--CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEE 4763 +GC CP+G + +G C+PP NG C+ A D C+ +C + C DG C+ + ++CD V DC + DE +C++ C L +FQC ++G C P +CD ++C D SDE+ CG C+ +Q+ C S C+ ++CDG C D SDE+ Sbjct: 1451 QGCLCPHGSLACADGRCLPPAL---LCNGHP---------------DCLDAA-----DEESCLGPVSCISGEVSCVDGTCVRTIQLCDGVWDCPDGADEGPSHCSLPSLPTPPGGIGQNPSTSSLDTAPSPVGSTSPASPCSLLEFQC-NSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQC--PDGSDED 1639 HSP 3 Score: 62.003 bits (149), Expect = 1.083e-8 Identity = 42/107 (39.25%), Postives = 48/107 (44.86%), Query Frame = 3 Query: 1581 CETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCG 1883 C QCPGN ++ C E CP C L Q P C S C C G + +C+ CPC GQ + PG V DCN CTC S CT K CP CG Sbjct: 2863 CTAQCPGNMVFRSAEQCLEEGGPCPQLC--LAQDPGVECTGSCAPSCNCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVAHLDCNNCTCISGEMVCTSKRCPVACG 2967 HSP 4 Score: 56.9954 bits (136), Expect = 3.037e-7 Identity = 40/132 (30.30%), Postives = 60/132 (45.45%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISR-------------TCIEEKKICDGNYDCGVNDKS----DEENC 4769 + C+ +F+C+ G+C CD+ DC++ DE +C C L Q C + C+ + CDG C D SDE C +R TCI+ +CDGN DC + D++ DE+ C Sbjct: 1566 SPCSLLEFQCNSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQCPDGSDEDPDVCEEQSASGGANRTGAPCPEFSCPDGTCIDFLLVCDGNPDCELADETEPSLDEQGC 1697 HSP 5 Score: 54.299 bits (129), Expect = 1.862e-6 Identity = 38/123 (30.89%), Postives = 55/123 (44.72%), Query Frame = 3 Query: 2766 CQSTCRELNEATA-SKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSC 3131 C+ TC E+N A S C + PGC C Y+++ TG CV E C CW+ P P G+ C++C C++G+ C +C C + +V M C DT + SC Sbjct: 4934 CEKTCPEMNMTQAWSNCTEAQAPGCVCQLGYFRSQTGLCVPEDHCECWHHGSPHLP----GSEWQEACESCRCLHGKSVCIR--HCPELSCAQGEVIMQEPGSCCPICQQDTLSTEEEEPVSC 5050
BLAST of SCO-spondin vs. Ensembl Mouse
Match: Sspo (SCO-spondin [Source:MGI Symbol;Acc:MGI:2674311]) HSP 1 Score: 436.802 bits (1122), Expect = 2.381e-124 Identity = 357/1233 (28.95%), Postives = 529/1233 (42.90%), Query Frame = 3 Query: 1260 VASFGNSFK--------------GFGCPDI-ANTLSTCKPDD-QILANQQCSKI-RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDV-GCQYVAVEPSVNNTDDRANVFVIIQS---NTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSC---DNTPQ--SIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITIN------CQTCVCI-NGRLNCKTKDNCTN--PVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVL----------WDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQF-AESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC---ITTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 A+FGNS+K +GC + TL+ + Q A C ++ F C V D Y C+ C + N + +C + Y+ +C+ + + WR CE CPG +++SDC S CP +C + Q C V C C +G G C+ CPC + Q + PG + CN C C WHC + +CP+ C + G + TFDG F F GC Y V+ SV + + V+++ +T S L ++V + + I L+ + V +DGED LP+ I + ++ ++ + + + L+ + YI + + GLCG F ++DF I S+ A + G C D +P S + IL+G F C VD+E + C + +D L C + C + G+ + + + L P+ GG V+Q C+ C C E + K C GC CPP GQCV S C C G TIN C C+C G NC T +C +CP+ +Y+ + C+S +C S+ + C CP TV +D CV P CPC + + Y PN I C +C + W+ + C C A G PHY T DG +F+F G+C Y L+ G ++SV N+ CG S TC K + + + + RG T+ I + ++ L GL +L WD TRV + L P F G V GLCGN+D D N+ E + A S+ N + PE P N R +WA C V+ +FA C H +V +YY C+YDACGC+ GGDCECLC+++AT+A +CA V+WR+Q LCP+ CEGG+ Y+ C S CP TC S HC CVEGC CP G + G C+ CPC + G + G +++ ++C C C ++++C + C C + C ++G C+ +CD DC + DE+ C +VC Q C S G C+P++ CDG +C D +DE C C + C R C+ +C+G+ DC D +DEE+C Sbjct: 224 AATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGPFWQCHGQVQPDEYHETCLFAYCVGATAGNGPE---GQLEAVCATFANYAQACARQHIYVHWRKPGFCERVCPGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGALCVPAAHCPCYHRRQRYAPGDTVKQQCNPCVCQDGRWHCAQALCPAECAVGGDGHYFTFDGRSFFFRGTPGCHYSLVQDSV-----KGQLLVVLEHGACDTGSCL------------HALSVFLGNTHIQLRYSG--------AVLVDGED--VDLPW-IGVEGFNISWASSTFLLLHWPGAWVLWGVAEPAAYITLDPRHAYQVQGLCGTFTWKQQDDFLTPAGDIETSVTAFASKFQVSGDGRCPLVDKSPLFCSSYSQHLTFTEAACAILHGHAFQECHG--LVDREPFRLRCLEAVCGCAPGRDCL--CPVLSAYTRHCAQEGVLLQ--WRNETL---CPVSCPGGQVYQECAPVCGHHCGEPEDC---KELGICVAGCNCPPGLLWDLEGQCVPPSMCHCQF----------GGHRYTINTTTVRDCSHCICQERGLWNC-TAHHCPRQWALCPRELIYVPGACLLTCDSPRANHSCWAGST--DGCVCPPGTV-LLDKHCVSPDLCPCRHNGQWYPPNATIQEDCNICVCQGQRWHCTGQ---RCSGWCQASGAPHYVTFDGLVFTFPGACEYLLVREAGGR------FSVSVQNLPCGASGLTCTKALVVRLDSTVVHMLRGQAVTVN---GVSIRLPKVYTGPGLSLHHAGLFLLLTTRLGLTLLWDGGTRVLVQLSPHFHGRVAGLCGNFDSDASNDLRSRQGVLEPTAELTAHSWRLNP------LCPEPGDLPHP------VNAHRANWARARCEVILQP--IFAPC----HTEVPPQQYYEWCVYDACGCDTGGDCECLCSAIATYADECARHRHHVRWRSQELCPLQCEGGQVYEPCGSTCPPTCHDHHSELRWHCQVITCVEGCFCPEGTLLHGGACMKLAACPCEWQGSFFPPGTVLQ-KDCGNCTCQGSQWHCDRGGAPCEDMEPGCAEGETLCRENGHCVPLEWLCDNQDDCGDGSDEEGCATSVCGEGQMSCQS-GHCLPLSLICDGQDDCGDGTDEQGCLCPHGSLACADGR-CLPPALLCNGHPDC--LDAADEESC 1364 HSP 2 Score: 76.6406 bits (187), Expect = 3.194e-13 Identity = 77/318 (24.21%), Postives = 115/318 (36.16%), Query Frame = 3 Query: 2139 DSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSH---FKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY--------------ITGSCDN------TPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYAT----YCRREQSSN--KDQL-SYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC 3002 +S D D P T+ D + + V S + D S SI I V GLCG ++G E+DF E + S+ + C++ T V A D+C +L GP F C + D E++ T YC + N + QL + C+ N A AC R I + C GG ++ C +C +C + + C +C GCECP + G CV + C C++ + P G + C CVC +GR +C Sbjct: 130 NSSAGSLEDPADELGVWPLTLNDPILFPGMSLQWQGDWLVLSGGLGVVVRLDRSSSISISVDHEFWGRTQGLCGLYNGRPEDDFVEPGGGLATLAATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGP-FWQCHGQVQPD-EYHETCLFAYCVGATAGNGPEGQLEAVCATFANYAQACARQHIYVHWRKPGFCERVC-----PGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGA-LCVPAAHCPCYHRRQRYAP----GDTVKQQCNPCVCQDGRWHC 435 HSP 3 Score: 65.4698 bits (158), Expect = 8.354e-10 Identity = 58/215 (26.98%), Postives = 89/215 (41.40%), Query Frame = 3 Query: 4242 EGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCI--TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDE--KNCNV----------------------------------CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG--CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEE 4763 +GC CP+G + +G C+PP NG C+ A D C+ +C + C DG C+ + ++CD V DC + DE +C++ C L +FQC ++G C P +CD ++C D SDE+ CG C+ +Q+ C S C+ ++CDG C D SDE+ Sbjct: 1326 QGCLCPHGSLACADGRCLPPAL---LCNGHP---------------DCLDAA-----DEESCLGPVSCISGEVSCVDGTCVRTIQLCDGVWDCPDGADEGPSHCSLPSLPTPPGGIGQNPSTSSLDTAPSPVGSTSPASPCSLLEFQC-NSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQC--PDGSDED 1514 HSP 4 Score: 62.003 bits (149), Expect = 9.781e-9 Identity = 42/107 (39.25%), Postives = 48/107 (44.86%), Query Frame = 3 Query: 1581 CETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCG 1883 C QCPGN ++ C E CP C L Q P C S C C G + +C+ CPC GQ + PG V DCN CTC S CT K CP CG Sbjct: 2718 CTAQCPGNMVFRSAEQCLEEGGPCPQLC--LAQDPGVECTGSCAPSCNCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVAHLDCNNCTCISGEMVCTSKRCPVACG 2822 HSP 5 Score: 56.9954 bits (136), Expect = 2.860e-7 Identity = 40/132 (30.30%), Postives = 60/132 (45.45%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISR-------------TCIEEKKICDGNYDCGVNDKS----DEENC 4769 + C+ +F+C+ G+C CD+ DC++ DE +C C L Q C + C+ + CDG C D SDE C +R TCI+ +CDGN DC + D++ DE+ C Sbjct: 1441 SPCSLLEFQCNSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQCPDGSDEDPDVCEEQSASGGANRTGAPCPEFSCPDGTCIDFLLVCDGNPDCELADETEPSLDEQGC 1572 HSP 6 Score: 54.299 bits (129), Expect = 2.074e-6 Identity = 36/112 (32.14%), Postives = 52/112 (46.43%), Query Frame = 3 Query: 2766 CQSTCRELNEATA-SKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDT 3098 C+ TC E+N A S C + PGC C Y+++ TG CV E C CW+ P P G+ C++C C++G+ C +C C + +V M C DT Sbjct: 4791 CEKTCPEMNMTQAWSNCTEAQAPGCVCQLGYFRSQTGLCVPEDHCECWHHGSPHLP----GSEWQEACESCRCLHGKSVCIR--HCPELSCAQGEVIMQEPGSCCPICQQDT 4896
BLAST of SCO-spondin vs. Ensembl Mouse
Match: Muc5ac (mucin 5, subtypes A and C, tracheobronchial/gastric [Source:MGI Symbol;Acc:MGI:104697]) HSP 1 Score: 398.282 bits (1022), Expect = 4.696e-112 Identity = 364/1291 (28.20%), Postives = 557/1291 (43.14%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA----SFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV-----EYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNG--SDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT-GSCDNTPQSIVDP-------PAGDNDICSTILN--GPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD--------SVSGC-NSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLM-DTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQL-----VSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-----EGG--KHYDACASPCPQTCDTIGSNAPSHC-STTHCVEGC--SCP-------NGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKME-NCKKCQCVQAKFNCTEDYSDCITTCTPSQFR 4454 C ++G +Y+TFD Q++ + G C+Y+ +A + ++I + S+T + + ++ +VV+ + + +L NN L + + + + + V+ + + + E ++ L+ D GLCG+ + +P ++ +NV FGN K G P P A ++ LK F GCA LV+ +Y C Q++C +LD SD +C L+ YS C+ G Q WR LC CP N +C S C TC N S C++ + C C G V + G C+ C CLY+G + PGT DC CTC+ W C + C TC +MG + TFDG ++ + C YV +P +N F ++ + L TE + K VT+ + G ++I++KAT VF++ T+LP + + I E + ++ +++ L+ GLCG F+ +DF + + ++ T +C N DP CS + N GP FS C + V+ + + C + + K + C+A+ + AC G+ + I C T +Q TCQ TCR LNE + C P GC CP + G+CV + C C+ K +P G ++ + C C G L C T PVC +Y D + +GC SC D +S C CPN V+ +G CV DCPC++ Y P + I + C C NRMW + C A C YGD HY T DGQ +SF G C YTL+ D G + ++ + NI CG + TC+K ++I +G LS + + + D+ E F Q+ V IGL +LWD+ T ++L L P F+G V GLCGN+D + N++ + D ++F S+ + CP + P +D C ANP RKSWA++ C ++ F++C H + +YY C+ DAC C+ GGDCEC CT++A +A C +G+ V WRT ++CP+ C EG HY C +PC +TC N C +EGC CP G + C T PC NG+ Y GA + + NC C C ++ CT + C+ T QF Sbjct: 80 CSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDAYEDFNIQLRRVQ-ESNTTTLSRVTMKLDGLVVELTKSSVLV----NNHPVQLPFSQSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYT-----------NKTCGLCGDFNGSPKSNEFLSNNVRLTPLEFGNLQKMDG-PTEQCQDPLPVPQKNCSARSGICEMILKGELFSGCAALVDISSYVEACRQDVCLCE-----SLDP---SDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPLNMQHQECGSPCVDTCSNPQH--SQVCEDHCIAGCFCPEGMVLDDINQMG--CVPVSQCACLYNGTLYAPGTNYSTDCTKCTCSGGQWSCQDIPCAGTCSVMGGSHMSTFDGRQYTVHG-DCTYVLSKPC------DSNAFTVLVELRKCGL-TESETCL----KTVTLNLGGGQTEIMVKATG--------EVFVN--QIYTQLPVSTANATFFRPSTFFIVGETNLGLQLEIQLSPIMQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNIFQDPCSLSVENEKYAQHWCSLLTNASGP-FSQCH--ATVNPSTFFSNCMYDTCNCEKSEDCMCAALSSYVRACAAKGVLLS-DWRDGICTKPTITCPKSMT-YQYHISTCQPTCRALNEKDVT-CHVSFIPVDGCTCPKGTFLDDLGKCVQATSCPCYY----KGSTVPNGESVQDSGAICTCTQGALTCIGGPAPT-PVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCYSSECVPGCVCPNGLVADGNGGCVVTEDCPCVHNEATYRPGETIQVGCNNCTCENRMWQCTDKP---CLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLSDSKMEVV----QKDVGQEPPYFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPS-CPDVLVP---------KDPCTANPYRKSWAQKQCSIINSE--TFSAC--HAHVEPAKYYEACVNDACACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQ----NPTGQCLQDLRGLEGCYPKCPPTAPIFDEGTMQCVSNCT--VTFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHNAGACVCTYNGQQFH 1281 HSP 2 Score: 125.561 bits (314), Expect = 4.362e-28 Identity = 113/408 (27.70%), Postives = 170/408 (41.67%), Query Frame = 3 Query: 3225 NDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRG----NNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQ-NEYMHGTMKYEDAIQFAESFIANADICPYEISPENNV-APSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFL-GIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCST--THCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 N V I K I R +N + VC +G+ HYKT DGQ+F F G C Y GD+ + I + + S T V + + G L++ NN + L F + ++IE + ++V+ +GL +W+ + L L + GLCG+++G + NE++ N + P E + P+++ P + A M +F+ C A D++ Y C D C C +C+C +LA ++ QCA G P WR NLC C + C SPC TC P H HC+ GC CP GM+ D+N CVP + C C YNG Y G + C KC C +++C + Sbjct: 54 NRVTIIPPLKTIPVVRAFN-----PGHTRRVCSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDAYED---FNIQLRRVQ--ESNTTTLSRVTMKLDGLVVELTKSSVLVNNHPVQLPFSQSGVLIELSNGYLKVVA-RLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKSNEFLSN----------------NVRLTPLEFGNLQKMDGPTEQCQDPLPVPQKNCSARSGICEMILKGELFSGC--AALVDISSYVEACRQDVCLCESLDPSDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPLNMQHQECGSPCVDTCSN-----PQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNGTLYAPGTNYSTD-CTKCTCSGGQWSCQD 426 HSP 3 Score: 88.9669 bits (219), Expect = 6.412e-17 Identity = 113/454 (24.89%), Postives = 172/454 (37.89%), Query Frame = 3 Query: 582 QLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAG----DVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXV-----ASFGNSFKG-FGCPDIANTLS---TCKPDDQILANQQCSKI---RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCET---QCPGNKIWSDCASECPVTCRNLFQTPSAYCQNS--KVGRCICGSGE-VEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPS 1874 Q+ C QC +Y C T S G S + C TC G + TFD + YT G C+Y+L+ P D +A T + + T C+K + + G +++VK++G + N T L V+ ++ P T V + + L ++ I G GLCGN ++ ++DF V A+F N+FK CP++ N + +++ A CS + F C VN T+FS C+ + C ++ D +C LS+Y +C+ KG + WR D +C CP + + S C TCR L + C S V C C G ++ C++ SCPC Y G P G Q CTC C P+ Sbjct: 384 QMGCVPVSQCACLYNGTLYAPGTNYSTDCTKCTCSGGQWSCQDIPCAG--------------TCSVMGGSHMSTFDGRQYTVHGDCTYVLSKPCDSNAFTVLVELRKCGLTESETCLKTVTLNLGGGQTEIMVKATGEVFV-----NQIYTQLPVSTANATFFRPSTFFIVGETNLGLQLEIQLSPI--------MQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNIFQDPCSLSVENEKYAQHWCSLLTNASGPFSQCHATVNPSTFFSNCMYDTCNCEKS----------EDCMCAALSSYVRACAAKGVLLSDWR-DGICTKPTITCPKSMTYQYHISTCQPTCRALNEK-DVTCHVSFIPVDGCTCPKGTFLDDLGKCVQATSCPCYYKGSTVPNGESVQDSGAICTCTQGALTCIGGPAPT 798 HSP 4 Score: 80.1073 bits (196), Expect = 3.226e-14 Identity = 107/416 (25.72%), Postives = 165/416 (39.66%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPS--DVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTC--KPDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLC---------ETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVG------RC-----ICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD--CNTCTCNSSLWHCTEKICPSTCGIMGTQF 1901 TC YG +Y TFD Q Y++ G C Y L + + + ++ EN T+ T C K I I G+ +K S DS + V D + P +V + N++++ + G + L WD + + G V GLCG ++ + S ++ FGNS+K CPD+ C P + A +QCS I + F C V Y+ CV + C D + CT ++AY+ +C + G C+ WR+ +C E QC + + C + C TC+N P+ C G +C I G ++ C + PC +G+ + PG D C++C C S CT C G QF Sbjct: 899 LATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLS--------DSK-----MEVVQKD---VGQEPPYFVHQMGNYLVVETDIG-LVLLWD-KKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPDVLVPKDPCTANPYRKSWAQKQCSIINSETFSACHAHVEPAKYYEACVNDAC--------ACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWH--YQPCGAPCMRTCQN----PTGQCLQDLRGLEGCYPKCPPTAPIFDEGTMQCVSNCTV--TFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHNAGACVCTYNGQQF 1280 HSP 5 Score: 70.8626 bits (172), Expect = 1.934e-11 Identity = 96/395 (24.30%), Postives = 161/395 (40.76%), Query Frame = 3 Query: 1854 TEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDD-RANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSE-NDFTEKDNSIRESIVNIAK-SYITGSCDNTPQSIVDPP---AGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQ--CVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCK 3005 T ++C ST G + TFDG F F + C YV + +D + + +SNT ++ VT+K++G + L ++++V ++ +H +LPF+ ++ + +L+N Y +V ++ S+ +++ N GLCG F+G+ + N+F N++R + + + G + + P + + IC IL G FSGC ++ VD Y CR++ S C + + C G Q + N+ PL Q C C TC N + C+D C GC CP Q CV S+C C P GTN + +C C C G+ +C+ Sbjct: 76 TRRVC-STWGNF--HYKTFDGQVFYFPGL-CNYVFSAHCGDAYEDFNIQLRRVQESNTTTL-------------SRVTMKLDGLVVEL---------TKSSVLVN--NHPVQLPFS---QSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKSNEFL--SNNVRLTPLEFGNLQKMDGPTEQCQDPLPVPQKNCSARSGICEMILKGELFSGC--AALVDISSYVEACRQDVCLCESLDPSDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPL----NMQHQECGSPCVDTCS--NPQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNGTLYAP----GTNYSTDCTKCTCSGGQWSCQ 425
BLAST of SCO-spondin vs. Ensembl Mouse
Match: Muc5ac (mucin 5, subtypes A and C, tracheobronchial/gastric [Source:MGI Symbol;Acc:MGI:104697]) HSP 1 Score: 394.045 bits (1011), Expect = 7.403e-111 Identity = 364/1291 (28.20%), Postives = 557/1291 (43.14%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA----SFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEV-----EYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNG--SDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT-GSCDNTPQSIVDP-------PAGDNDICSTILN--GPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD--------SVSGC-NSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLM-DTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQL-----VSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-----EGG--KHYDACASPCPQTCDTIGSNAPSHC-STTHCVEGC--SCP-------NGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKME-NCKKCQCVQAKFNCTEDYSDCITTCTPSQFR 4454 C ++G +Y+TFD Q++ + G C+Y+ +A + ++I + S+T + + ++ +VV+ + + +L NN L + + + + + V+ + + + E ++ L+ D GLCG+ + +P ++ +NV FGN K G P P A ++ LK F GCA LV+ +Y C Q++C +LD SD +C L+ YS C+ G Q WR LC CP N +C S C TC N S C++ + C C G V + G C+ C CLY+G + PGT DC T TC+ W C + C TC +MG + TFDG ++ + C YV +P +N F ++ + L TE + K VT+ + G ++I++KAT VF++ T+LP + + I E + ++ +++ L+ GLCG F+ +DF + + ++ T +C N DP CS + N GP FS C + V+ + + C + + K + C+A+ + AC G+ + I C T +Q TCQ TCR LNE + C P GC CP + G+CV + C C+ K +P G ++ + C C G L C T PVC +Y D + +GC SC D +S C CPN V+ +G CV DCPC++ Y P + I + C C NRMW + C A C YGD HY T DGQ +SF G C YTL+ D G + ++ + NI CG + TC+K ++I +G LS + + + D+ E F Q+ V IGL +LWD+ T ++L L P F+G V GLCGN+D + N++ + D ++F S+ + CP + P +D C ANP RKSWA++ C ++ F++C H + +YY C+ DAC C+ GGDCEC CT++A +A C +G+ V WRT ++CP+ C EG HY C +PC +TC N C +EGC CP G + C T PC NG+ Y GA + + NC C C ++ CT + C+ T QF Sbjct: 80 CSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDAYEDFNIQLRRVQ-ESNTTTLSRVTMKLDGLVVELTKSSVLV----NNHPVQLPFSQSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYT-----------NKTCGLCGDFNGSPKSNEFLSNNVRLTPLEFGNLQKMDG-PTEQCQDPLPVPQKNCSARSGICEMILKGELFSGCAALVDISSYVEACRQDVCLCE-----SLDP---SDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPLNMQHQECGSPCVDTCSNPQH--SQVCEDHCIAGCFCPEGMVLDDINQMG--CVPVSQCACLYNGTLYAPGTNYSTDC-TNTCSGGQWSCQDIPCAGTCSVMGGSHMSTFDGRQYTVHG-DCTYVLSKPC------DSNAFTVLVELRKCGL-TESETCL----KTVTLNLGGGQTEIMVKATG--------EVFVN--QIYTQLPVSTANATFFRPSTFFIVGETNLGLQLEIQLSPIMQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNIFQDPCSLSVENEKYAQHWCSLLTNASGP-FSQC--HATVNPSTFFSNCMYDTCNCEKSEDCMCAALSSYVRACAAKGVLLS-DWRDGICTKPTITCPKSMT-YQYHISTCQPTCRALNEKDVT-CHVSFIPVDGCTCPKGTFLDDLGKCVQATSCPCYY----KGSTVPNGESVQDSGAICTCTQGALTCIGGPAPT-PVCDAPMIYFDCHNATPGDTGAGCQKSCHTLDMTCYSSECVPGCVCPNGLVADGNGGCVVTEDCPCVHNEATYRPGETIQVGCNNCTCENRMWQCTDKP---CLATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLSDSKMEVV----QKDVGQEPPYFVHQMGNYLVVETDIGLVLLWDKKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPS-CPDVLVP---------KDPCTANPYRKSWAQKQCSIINSE--TFSAC--HAHVEPAKYYEACVNDACACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWHYQPCGAPCMRTCQ----NPTGQCLQDLRGLEGCYPKCPPTAPIFDEGTMQCVSNCT--VTFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHNAGACVCTYNGQQFH 1280 HSP 2 Score: 123.635 bits (309), Expect = 1.707e-27 Identity = 117/429 (27.27%), Postives = 172/429 (40.09%), Query Frame = 3 Query: 3225 NDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRG----NNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQ-NEYMHGTMKYEDAIQFAESFIANADICPYEISPENNV-APSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFL-GIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCST--THCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKC 4472 N V I K I R +N + VC +G+ HYKT DGQ+F F G C Y GD+ + I + + S T V + + G L++ NN + L F + ++IE + ++V+ +GL +W+ + L L + GLCG+++G + NE++ N + P E + P+++ P + A M +F+ C A D++ Y C D C C +C+C +LA ++ QCA G P WR NLC C + C SPC TC P H HC+ GC CP GM+ D+N CVP + C C YNG Y G T +DC TC+ Q+ C D C Sbjct: 54 NRVTIIPPLKTIPVVRAFN-----PGHTRRVCSTWGNFHYKTFDGQVFYFPGLCNYVFSAHCGDAYED---FNIQLRRVQ--ESNTTTLSRVTMKLDGLVVELTKSSVLVNNHPVQLPFSQSGVLIELSNGYLKVVA-RLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKSNEFLSN----------------NVRLTPLEFGNLQKMDGPTEQCQDPLPVPQKNCSARSGICEMILKGELFSGC--AALVDISSYVEACRQDVCLCESLDPSDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPLNMQHQECGSPCVDTCSN-----PQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNGTLYAPG--------------------TNYSTDCTNTCSGGQWSCQDIPC 428 HSP 3 Score: 89.3521 bits (220), Expect = 4.988e-17 Identity = 112/456 (24.56%), Postives = 171/456 (37.50%), Query Frame = 3 Query: 582 QLYCYQSDQCFAFKKSDFLGKAVSYDECCAD--KTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAG----DVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXV-----ASFGNSFKG-FGCPDIANTLS---TCKPDDQILANQQCSKI---RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLC---ETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNS--KVGRCICGSGE-VEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPS 1874 Q+ C QC +Y C + G W Q C TC G + TFD + YT G C+Y+L+ P D +A T + + T C+K + + G +++VK++G + N T L V+ ++ P T V + + L ++ I G GLCGN ++ ++DF V A+F N+FK CP++ N + +++ A CS + F C VN T+FS C+ + C ++ D +C LS+Y +C+ KG + WR D +C CP + + S C TCR L + C S V C C G ++ C++ SCPC Y G P G Q CTC C P+ Sbjct: 384 QMGCVPVSQCACLYNGTLYAPGTNYSTDCTNTCSGGQWSCQDIPCAG-----------------TCSVMGGSHMSTFDGRQYTVHGDCTYVLSKPCDSNAFTVLVELRKCGLTESETCLKTVTLNLGGGQTEIMVKATGEVFV-----NQIYTQLPVSTANATFFRPSTFFIVGETNLGLQLEIQLSPI--------MQTSVRLKPGLRGLTCGLCGNFNSMQADDFQTISGVVEGTAAAFFNTFKTQAACPNVKNIFQDPCSLSVENEKYAQHWCSLLTNASGPFSQCHATVNPSTFFSNCMYDTCNCEKS----------EDCMCAALSSYVRACAAKGVLLSDWR-DGICTKPTITCPKSMTYQYHISTCQPTCRALNEK-DVTCHVSFIPVDGCTCPKGTFLDDLGKCVQATSCPCYYKGSTVPNGESVQDSGAICTCTQGALTCIGGPAPT 797 HSP 4 Score: 80.8777 bits (198), Expect = 1.785e-14 Identity = 107/416 (25.72%), Postives = 165/416 (39.66%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPS--DVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTC--KPDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLC---------ETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVG------RC-----ICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD--CNTCTCNSSLWHCTEKICPSTCGIMGTQF 1901 TC YG +Y TFD Q Y++ G C Y L + + + ++ EN T+ T C K I I G+ +K S DS + V D + P +V + N++++ + G + L WD + + G V GLCG ++ + S ++ FGNS+K CPD+ C P + A +QCS I + F C V Y+ CV + C D + CT ++AY+ +C + G C+ WR+ +C E QC + + C + C TC+N P+ C G +C I G ++ C + PC +G+ + PG D C++C C S CT C G QF Sbjct: 898 LATCAVYGDGHYITFDGQRYSFNGDCEYTLLQDNCGGNGSSQDAFRVITENIPCGTTGTTCSKSIKIFLGNYELKLS--------DSK-----MEVVQKD---VGQEPPYFVHQMGNYLVVETDIG-LVLLWD-KKTSIFLRLSPEFKGRVCGLCGNFDDNAINDFTTRSQSVVSDMLEFGNSWKLSPSCPDVLVPKDPCTANPYRKSWAQKQCSIINSETFSACHAHVEPAKYYEACVNDAC--------ACDSGGDCECFCTTVAAYAQACHEVGVCVSWRTPDICPLFCDYYNPEGQCEWH--YQPCGAPCMRTCQN----PTGQCLQDLRGLEGCYPKCPPTAPIFDEGTMQCVSNCTV--TFPCRVNGKLYRPGASVPSDKNCDSCICTESGVRCTHNAGACVCTYNGQQF 1279 HSP 5 Score: 65.855 bits (159), Expect = 6.035e-10 Identity = 96/395 (24.30%), Postives = 161/395 (40.76%), Query Frame = 3 Query: 1854 TEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDD-RANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSE-NDFTEKDNSIRESIVNIAK-SYITGSCDNTPQSIVDPP---AGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQ--CVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCK 3005 T ++C ST G + TFDG F F + C YV + +D + + +SNT ++ VT+K++G + L ++++V ++ +H +LPF+ ++ + +L+N Y +V ++ S+ +++ N GLCG F+G+ + N+F N++R + + + G + + P + + IC IL G FSGC ++ VD Y CR++ S C + + C G Q + N+ PL Q C C TC N + C+D C GC CP Q CV S+C C P GTN + +C T C G+ +C+ Sbjct: 76 TRRVC-STWGNF--HYKTFDGQVFYFPGL-CNYVFSAHCGDAYEDFNIQLRRVQESNTTTL-------------SRVTMKLDGLVVEL---------TKSSVLVN--NHPVQLPFS---QSGVLIELSNGYLKVVARLGLLFVWNEDDSLLLELDTKYTNKTCGLCGDFNGSPKSNEFL--SNNVRLTPLEFGNLQKMDGPTEQCQDPLPVPQKNCSARSGICEMILKGELFSGC--AALVDISSYVEACRQDVCLCESLDPSDCICHTLAEYSRQCAHAGGQPQDWRGPNLCSQTCPL----NMQHQECGSPCVDTCS--NPQHSQVCEDHCIAGCFCPEGMVLDDINQMGCVPVSQCACLYNGTLYAP----GTNYSTDC-TNTCSGGQWSCQ 424 HSP 6 Score: 52.373 bits (124), Expect = 8.133e-6 Identity = 46/178 (25.84%), Postives = 70/178 (39.33%), Query Frame = 3 Query: 1383 FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDT--LCETQCPGNKIWSDCASECPVTC-RNLFQTPSAYCQNS--KVGRCICGSGEVEYGYT----------CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICP 1871 F C D++ ++ C+ + C LD+ +V+C+ L Y+ C+ +G CI WRS T C CP N+++ C P C R+ +PS Q + K C C + C+ P G+ PG DC C C + C +K CP Sbjct: 2253 FKLCHDVIPPLQFYQGCLFDYCH-------MLDL----EVVCSGLELYASLCAAQGVCIPWRSQTNNTCSFTCPDNQVYQPCGPSNPHYCYRDDSISPSLTLQEAGPKTEGCFCPDSTTLFSTNDSICVPSCQWCLGPR-------GEPVEPGHTISIDCQDCICKEATLTCQKKACP 2412
BLAST of SCO-spondin vs. UniProt/SwissProt
Match: sp|Q2PC93|SSPO_CHICK (SCO-spondin OS=Gallus gallus OX=9031 GN=SSPO PE=2 SV=1) HSP 1 Score: 559.681 bits (1441), Expect = 7.016e-164 Identity = 453/1539 (29.43%), Postives = 682/1539 (44.31%), Query Frame = 3 Query: 606 QCFA-FKKSDFLGK----AVSYDECCADKTG-SWGSQSGS-CVSCKANFAGGN-----------DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAG-NIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXX-----XXXXXXXXXVASFGNSFK------GFGCPDIANTLSTCKPDD--QILANQQCSKIRL-KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-------ITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKC---DDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYM--DSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTI--------PLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITT--CTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDE---ISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC-KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 QC A ++ D LG VS ECC G SW + S + C +C G+ + +C + YR+FD + + +QG C+Y L A +D TW+++I G V+ + G V + G+ I N P + + V +F+ ++E+G + LK D +++++ + G GLCG +++P + ASFGNS++ C D C Q A C + F C + V+ ++ C++ C + PS +C L+ Y C+ + + I+WR LCE QC + +SDC S CP +C +C++ C C G + CI +CPCL+ G + PG + CN CTC W CT+ C + C ++G + TFD +F+F C+Y V+ V T + I + E+ G + S + +++ V G+ L +T V +DG LPF + VR+ ++ + ++ L+ + + YI + A N + GLCG ++ + +DF + + A Y + P S P IL+G F C + VD+E + C + + + C A+ A C + G S C GG V+Q CS C TC +L AS C D+ C GC CP GQCV C C + S+ PAG+ I C C+C G +C CP + VY+ + C+S TCT IA+ C CP TV +D CV P +CPC + R Y PND I C +C + W SE +C C+A GDPHY T DG+ FSF G C Y L+ +N + T++ N+ CG S TC K V + +G + RG + T+ ++ + +++ F L+S L VLWD TRVY+ L+P +G V GLCGN+D D +N+ E + + + +CP V + Q C NP R +WA + C ++ T +FA C H +V +Y C++DACGC+ GGDCECLCT++AT+A +C+ GI ++WR+Q+LCP+ C+GG+ Y AC PCPQTC +G P HC T C+EGC CP G + G C+ P CPCF+ G ++ + A+++ + C+ C C + C C C S+F C G+C+ + +CD DC + DE C Q +C ++G C+P +CDG +C D SDE C P + C R CI +C+G DCG D SDE C C + E++C + C+ ++C+G DDCGD SDE GC C + + QC+ CDG+ DC +G DE C Sbjct: 121 QCHASWQCQDALGAHNLSTVSMAECCRQPWGHSWRNGSSALCFACSRQPLTGDVPLPTAPRGPAARHRGPTASCTVWAGSRYRSFDGRHFGFQGECAYSLAASTDS---TWAVSIT-------PGSPPVLHMTFGLDTVVAQGHNI----SVNGVAVPEGRQHLHGGI-------SVTWLGDFV--AVESGLGVHLKLDGRGTVYVTVSAELR-GSTKGLCGPYNDDPIDDFLRVEGDVAPLAASFGNSWRIPDANPELSCSDAVEPSPGCAMGSTAQRAAEAMCGMLLTDPFRQCHEAVDPHGFYEACLELHCREGGTG------PSPPPAVCDTLATYVRDCAQRRAYIEWRRPGLCEQQCGHGQRYSDCVSSCPASCMAAGTAEEGHCRDDCASGCECTPGLLLDRGACIPQSACPCLHRGHIYAPGQSIRQRCNQCTCRGGRWLCTQDRCAAECAVLGDLHYITFDRRRFSFPGA-CEYTLVQDFVEGT-------LRITAEQEACGGHQPLSCL----RALSITVPGASARLHSTG--------EVVVDGR--VVPLPFA--SAALTVRRASSSFLLLQTFGAHLLWGLETPAAYITLQPAFANKVRGLCGTYNWDQRDDFATPAGDVEVGVTAFANKYRVSTDCPVLSPVPFEPCSTYAPRRELAAAACAILHGASFQPCHH--LVDREPFHQLCLYDVCACPAGKHCLCPALAAYARECAQEGAALSWRNESFCGTQC-----RGGQVYQECSSPCGRTCADLRLDGASSCPSLDNICVSGCNCPEGPVLDDGGQCVPPGVCPCQHSSQ----LYPAGSKIRQGCNACMCTAGTWSCTDAPCPDAAFCPGDLVYVFGSCLRTCDSAEPNGTCT---GIADGCVCPPGTVF-LDERCVPPEECPCQHNGRLYHPNDTIVRDCNTCVCRQQRWQCSSE---DCMGTCVATGDPHYITFDGRAFSFLGDCEYVLVR----EANGL--FTVTAENVPCGTSGVTCTKSVVVEMGNTVVHMLRGRDVTVNGVSVRPPKVYSGNGLTLQRAGIFLLLLSRLG-LAVLWDGGTRVYIRLQPQHRGRVVGLCGNFDRDAENDLASQQGVLEPTAELFGNSWRVSLLCP-------EVDGTTAQHPCTDNPHRATWARKRCSIL--TQRLFAPC----HDEVPCQHFYDWCIFDACGCDSGGDCECLCTAIATYAEECSQRGIHIRWRSQDLCPMQCDGGQEYSACGPPCPQTCRNLGLELPEHCDTMSCLEGCFCPEGKVLHEGSCIDPAECPCFWQGIAFPDSAVVQ-QGCRNCSCTAGLWQCVPTAEPCPAQPHCPDSEFPCRSGGRCVPGAWLCDNEDDCGDGSDEVCALHCAPHQHRC-ADGQCVPWGARCDGLSDCGDGSDERGCPPPPCAPPEFRCASGR-CIPRAHVCNGELDCGFADDSDEAGCSPSCSVGEFQCAAGR-CVPYPHRCNGHDDCGDFSDERGCVC-------------PAGHFQCPDAQCLPPAALCDGMQDCGDGTDEAFC 1550 HSP 2 Score: 72.4034 bits (176), Expect = 5.452e-11 Identity = 60/229 (26.20%), Postives = 81/229 (35.37%), Query Frame = 3 Query: 4431 TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCN------------------------VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC--GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 TC P Q C DG C+ K+CD + DC + DE + VC +F C S G C+P CD +C DNSDE+ C C+ C + CI +CDG C + ++NC Q+ C +C NN+ C+N T+ CDG DC G DE CE + Sbjct: 1555 TCAPGQLPCPDGSCVSQVKLCDGIWDCRDGWDESSVRCMVSWAPPAPTQLPTVPANGTAAPVCGPYEFPCRS-GQCVPRGWVCDSEADCPDNSDELGCNRSCVLGHFPCALGAHCIHYDHLCDGIPHCPDHSDESDDNCGSTQIP--PCPGHFVC---NNRV-----------------------------------------CVNATRVCDGALDCPQGEDELACEGY 1736 HSP 3 Score: 56.6102 bits (135), Expect = 2.712e-6 Identity = 32/92 (34.78%), Postives = 44/92 (47.83%), Query Frame = 3 Query: 4176 CPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQF 4451 CP C +G+ T C GC CP G++ NG CVPP+ C C + G Y G I ++ C C CV + C+ + C CT S + Sbjct: 2980 CPWICQDLGAGV---ACTAQCQPGCHCPAGLLLQNGTCVPPSHCLCHHRGHLYQPGDINALDTCNNCTCVTGQMVCSTE--TCPVPCTWSNW 3066 HSP 4 Score: 55.4546 bits (132), Expect = 6.280e-6 Identity = 40/147 (27.21%), Postives = 62/147 (42.18%), Query Frame = 3 Query: 2766 CQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSI-----------ANSCGCPNDTVSKIDGTC 3173 C+ +CR++ + T + C PGC C P +Y+ +G CV + C C + + P G+ C C C++G+ NC D CT CP+ +V + C + + SS+ C PN VS G C Sbjct: 5049 CEQSCRDILDETPANCTPSPSPGCTCEPGHYRNSSGHCVPSTLCECLHQGQLHQP----GSEWQEQCARCRCVDGKANC--TDGCTPLSCPEGEVKVREPGRCCPVCRMEWPEEPSSMCRRFTELRNITKGPCSLPNVEVSFCSGRC 5189
BLAST of SCO-spondin vs. UniProt/SwissProt
Match: sp|Q700K0|SSPO_RAT (SCO-spondin OS=Rattus norvegicus OX=10116 GN=Sspo PE=2 SV=1) HSP 1 Score: 480.715 bits (1236), Expect = 7.537e-138 Identity = 408/1457 (28.00%), Postives = 615/1457 (42.21%), Query Frame = 3 Query: 663 CCADKTGS--WGSQSGSCVSCKANFAGGN------------------DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYE-NCTSDTGCVKVIVIEAGDVVVKSSG----NGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQD--NFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXX-----XXXXXXXXVASFGNSFK--GF--GCPDIANTLSTCKPD-DQILANQQCSKIRLK------------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFN-DVGCQYVAVEPSVNNTDDRANVFVIIQSNT-ESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSC---DNTPQS---IVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSS---NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITI-NCQTCVCI-NGRLNCKTKDNCTN--PVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVL----------WDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITT---CTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNCN--VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 CCA G W S S C+SC GN TC ++ +Y+TFD Q Y + G+C+Y+L D TW++++ +C C V VI + V+ G G + L +S+ L++ G V+R D + I++S++ + G GLCG + P A+FGNS++ G+ GC D C+ + L + K++ + F C V D Y C+ C + + + +C + Y+ +C+ + + WR CE CPG +++SDC S CP +C + Q C V C C +G G C+ CPC Y Q + PG + CN C C WHC + CP+ C + G + TFDG F+F + GCQY V+ SV + + V+++ E+ SA++ + I ++ +G+ V +DG+D LP+ I + +V ++ + ++ + L+ ++YI + + GLCG F ++DF I S+ A + G C DNTP S + L+G F C V++E + C S +D L CS + A C + G+ + N +P GG V+Q C+ C C E + K C GC CPP GQCV S C C + T T+ +C C+C G NC +C +CP+ +Y + C+S +C S+ + C CP+ TV +D CV P CPC + + Y PN I C +C N+ W+ + C C A G PHY T DG +F+F G+C Y L+ G ++S+ N+ CG S TC K + + + + RG T+ I + ++ L GL +L WD TRV + L P F G V GLCGN+DGD+ N+ E + +CP P C+ N R +WA C V+ +FA C H +V +YY C+YDACGC+ GGDCECLC+++AT+A +CA V+WR+Q LCP+ CEGG+ Y+ C S CP TC HC CVEGC CP G + G CV T CPC + G + GA+++ ++C C C +++++C + C C + C + G C+ +CD DC + DE+ C+ VC Q C S G C+P++ CDG +C D +DE C C + C R C+ +CDG+ DC D +DEE+C Sbjct: 139 CCAQPWGHSWWNSSSQMCLSCSGQHRPGNASSEGLLQPLAGAVGQLWSQRQRPSATCATWSGFHYQTFDGQHYHFLGQCTYLLAGAMDS---TWAVHLRPSVHCPQPRQCWLVQVIMGPEEVLIQDGEVSVKGQPVPVGESQLLHGMSLQWQGDWLVLSGGLGVVVRLDRSSSIIISVD--------------------HEFWGRTQGLCGLYNGRPEDDFVEPGGGLAMLAATFGNSWRLPGYEPGCLDTVEVARGCEGLLEGTLTGLEAGKLQAQAQDLCHQLLEDPFSQCHGQVPPDEYHETCLFAYCVGATAGSGPEEQ---VKAVCATFANYAQACARQHIYVHWRKPGFCERLCPGGQLYSDCISSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGALCVPAAHCPCYYRRQRYAPGDTVKQQCNPCVCQDGRWHCAQAPCPAECAVGGDGHYCTFDGRSFSFRGNPGCQYSLVQDSV-----KGQLLVVLEHGACETGSCLHALSAFLGKTHI-QLRYSGA-----------------VLVDGQD--VDLPW-IGAEGFNVSHASSTFLLLRWPGAWVLWGVADPAVYITLDPRHAYQVQGLCGTFTWKQQDDFLTPAGDIETSVTAFASKFQVSGDGRCPLVDNTPLSSCSTYSQRLAFAEAACAALHGHAFQECHG--LVEREPFRLRCLESMCSCAPGRDCL--CSVLSAYAHHCAQEGVLLQW---RNETLCSVPC--PGGQVYQECAPACGHYCGEPEDC---KELGSCVAGCNCPPGLLWDLEGQCVPPSMCPC----QLGGHRYAFNTTTTLKDCSHCICQERGLWNC-IAHHCPRQWALCPQELIYAPGACLLTCDSLGANHSCLAGST--DGCVCPSGTV-LLDKHCVSPDLCPCRHNGQWYPPNATIQEDCNICVCQNQRWHCTGQ---RCSGWCQASGAPHYVTFDGLVFTFPGACEYLLVREAGGR------FSVSIQNLPCGASGLTCTKALAVRLDSTVVHMLRGQAVTVN---GVSIKLPKVYTGPGLSLHHAGLFLLLTTRLGLTLLWDGGTRVLVQLSPHFHGRVTGLCGNFDGDVSNDLRSRQGVLEPTAELTAHSWRLNPLCP---------EPGDLPHPCSVNAHRVNWARAHCEVILQP--IFAPC----HTEVPPQQYYEWCVYDACGCDTGGDCECLCSAIATYADECARHRHHVRWRSQELCPLQCEGGQVYEPCGSTCPPTCHDHHPELRWHCQAITCVEGCFCPEGTLLHGGTCVELTDCPCEWQGSFFPPGAVLQ-KDCGNCTCQESQWHCNPSGAPCEEMEPGCAEGEALCRESGHCVPLEWLCDNQDDCGDGSDEEGCDTSVCGEGQMSCQS-GRCLPLSLICDGQDDCGDGTDEQGCLCPQGFLACADGR-CLPPALLCDGHPDC--LDAADEESC 1491 HSP 2 Score: 62.003 bits (149), Expect = 7.578e-8 Identity = 41/107 (38.32%), Postives = 50/107 (46.73%), Query Frame = 3 Query: 1581 CETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCG 1883 C QCPGN ++ C E CP C L Q P C S C C G + +C+ CPC GQ + PG V + DCN CTC+S CT + CP CG Sbjct: 2867 CTAQCPGNMVFRSAEKCLEEGGPCPQLC--LAQDPGVECTGSCAPGCSCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVARLDCNNCTCSSGEMVCTSERCPVACG 2971 HSP 3 Score: 57.3806 bits (137), Expect = 1.905e-6 Identity = 40/132 (30.30%), Postives = 61/132 (46.21%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGC-------------LPHQIVCNISRTCIEEKKICDGNYDCGVNDKS----DEENC 4769 + C+ S+F+C+ G+C CD+ DC++ DE +C C L Q C C+ + CDG C D SDE C +P + TCI+ +CDG+ DC + D++ DE+ C Sbjct: 1568 SPCSLSEFQCNSGECTPRGWRCDREEDCTDGSDELDCGGPCKLYQMPCAHGPHCLSPGQLCDGVAQCPDGSDEDPDVCEERSASGGPNGTAVPCPEFSCPNGTCIDFLLVCDGSPDCELADETEPSLDEQGC 1699 HSP 4 Score: 55.8398 bits (133), Expect = 4.677e-6 Identity = 37/123 (30.08%), Postives = 58/123 (47.15%), Query Frame = 3 Query: 2736 GTVFQICS-RTCQSTCRELNEATA-SKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDT 3098 G + Q+ R C+ TC E+N+ A S C + PGC C ++++ TG CV E C CW+ P P G+ C++C C++G+ C +C C + +V + C DT Sbjct: 4923 GEILQLGELRPCEKTCLEMNKTQAWSNCTEAQVPGCVCQLGHFRSHTGLCVPEDHCECWHHGSPHLP----GSEWQEACESCRCLHGKSVC--TQHCPELSCAQGEVVVQEPGSCCPICQQDT 5039
BLAST of SCO-spondin vs. UniProt/SwissProt
Match: sp|Q8CG65|SSPO_MOUSE (SCO-spondin OS=Mus musculus OX=10090 GN=Sspo PE=2 SV=2) HSP 1 Score: 436.802 bits (1122), Expect = 1.666e-123 Identity = 357/1233 (28.95%), Postives = 529/1233 (42.90%), Query Frame = 3 Query: 1260 VASFGNSFK--------------GFGCPDI-ANTLSTCKPDD-QILANQQCSKI-RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDV-GCQYVAVEPSVNNTDDRANVFVIIQS---NTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSC---DNTPQ--SIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITIN------CQTCVCI-NGRLNCKTKDNCTN--PVCPKNQVYMDS--VSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVL----------WDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQF-AESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC---ITTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 A+FGNS+K +GC + TL+ + Q A C ++ F C V D Y C+ C + N + +C + Y+ +C+ + + WR CE CPG +++SDC S CP +C + Q C V C C +G G C+ CPC + Q + PG + CN C C WHC + +CP+ C + G + TFDG F F GC Y V+ SV + + V+++ +T S L ++V + + I L+ + V +DGED LP+ I + ++ ++ + + + L+ + YI + + GLCG F ++DF I S+ A + G C D +P S + IL+G F C VD+E + C + +D L C + C + G+ + + + L P+ GG V+Q C+ C C E + K C GC CPP GQCV S C C G TIN C C+C G NC T +C +CP+ +Y+ + C+S +C S+ + C CP TV +D CV P CPC + + Y PN I C +C + W+ + C C A G PHY T DG +F+F G+C Y L+ G ++SV N+ CG S TC K + + + + RG T+ I + ++ L GL +L WD TRV + L P F G V GLCGN+D D N+ E + A S+ N + PE P N R +WA C V+ +FA C H +V +YY C+YDACGC+ GGDCECLC+++AT+A +CA V+WR+Q LCP+ CEGG+ Y+ C S CP TC S HC CVEGC CP G + G C+ CPC + G + G +++ ++C C C ++++C + C C + C ++G C+ +CD DC + DE+ C +VC Q C S G C+P++ CDG +C D +DE C C + C R C+ +C+G+ DC D +DEE+C Sbjct: 224 AATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGPFWQCHGQVQPDEYHETCLFAYCVGATAGNGPE---GQLEAVCATFANYAQACARQHIYVHWRKPGFCERVCPGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGALCVPAAHCPCYHRRQRYAPGDTVKQQCNPCVCQDGRWHCAQALCPAECAVGGDGHYFTFDGRSFFFRGTPGCHYSLVQDSV-----KGQLLVVLEHGACDTGSCL------------HALSVFLGNTHIQLRYSG--------AVLVDGED--VDLPW-IGVEGFNISWASSTFLLLHWPGAWVLWGVAEPAAYITLDPRHAYQVQGLCGTFTWKQQDDFLTPAGDIETSVTAFASKFQVSGDGRCPLVDKSPLFCSSYSQHLTFTEAACAILHGHAFQECHG--LVDREPFRLRCLEAVCGCAPGRDCL--CPVLSAYTRHCAQEGVLLQ--WRNETL---CPVSCPGGQVYQECAPVCGHHCGEPEDC---KELGICVAGCNCPPGLLWDLEGQCVPPSMCHCQF----------GGHRYTINTTTVRDCSHCICQERGLWNC-TAHHCPRQWALCPRELIYVPGACLLTCDSPRANHSCWAGST--DGCVCPPGTV-LLDKHCVSPDLCPCRHNGQWYPPNATIQEDCNICVCQGQRWHCTGQ---RCSGWCQASGAPHYVTFDGLVFTFPGACEYLLVREAGGR------FSVSVQNLPCGASGLTCTKALVVRLDSTVVHMLRGQAVTVN---GVSIRLPKVYTGPGLSLHHAGLFLLLTTRLGLTLLWDGGTRVLVQLSPHFHGRVAGLCGNFDSDASNDLRSRQGVLEPTAELTAHSWRLNP------LCPEPGDLPHP------VNAHRANWARARCEVILQP--IFAPC----HTEVPPQQYYEWCVYDACGCDTGGDCECLCSAIATYADECARHRHHVRWRSQELCPLQCEGGQVYEPCGSTCPPTCHDHHSELRWHCQVITCVEGCFCPEGTLLHGGACMKLAACPCEWQGSFFPPGTVLQ-KDCGNCTCQGSQWHCDRGGAPCEDMEPGCAEGETLCRENGHCVPLEWLCDNQDDCGDGSDEEGCATSVCGEGQMSCQS-GHCLPLSLICDGQDDCGDGTDEQGCLCPHGSLACADGR-CLPPALLCNGHPDC--LDAADEESC 1364 HSP 2 Score: 76.6406 bits (187), Expect = 2.234e-12 Identity = 77/318 (24.21%), Postives = 115/318 (36.16%), Query Frame = 3 Query: 2139 DSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSH---FKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY--------------ITGSCDN------TPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYAT----YCRREQSSN--KDQL-SYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC 3002 +S D D P T+ D + + V S + D S SI I V GLCG ++G E+DF E + S+ + C++ T V A D+C +L GP F C + D E++ T YC + N + QL + C+ N A AC R I + C GG ++ C +C +C + + C +C GCECP + G CV + C C++ + P G + C CVC +GR +C Sbjct: 130 NSSAGSLEDPADELGVWPLTLNDPILFPGMSLQWQGDWLVLSGGLGVVVRLDRSSSISISVDHEFWGRTQGLCGLYNGRPEDDFVEPGGGLATLAATFGNSWKLPGSEPGCLDAVEVAWGCESLLGGTLTDLEAVKLQAQAQDMCHQLLEGP-FWQCHGQVQPD-EYHETCLFAYCVGATAGNGPEGQLEAVCATFANYAQACARQHIYVHWRKPGFCERVC-----PGGQLYSDCVSSCPPSCSAVAQGEEGSCGKECVSGCECPTGLFWDGA-LCVPAAHCPCYHRRQRYAP----GDTVKQQCNPCVCQDGRWHC 435 HSP 3 Score: 65.4698 bits (158), Expect = 5.843e-9 Identity = 58/215 (26.98%), Postives = 89/215 (41.40%), Query Frame = 3 Query: 4242 EGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCI--TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDE--KNCNV----------------------------------CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG--CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEE 4763 +GC CP+G + +G C+PP NG C+ A D C+ +C + C DG C+ + ++CD V DC + DE +C++ C L +FQC ++G C P +CD ++C D SDE+ CG C+ +Q+ C S C+ ++CDG C D SDE+ Sbjct: 1326 QGCLCPHGSLACADGRCLPPAL---LCNGHP---------------DCLDAA-----DEESCLGPVSCISGEVSCVDGTCVRTIQLCDGVWDCPDGADEGPSHCSLPSLPTPPGGIGQNPSTSSLDTAPSPVGSTSPASPCSLLEFQC-NSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQC--PDGSDED 1514 HSP 4 Score: 62.003 bits (149), Expect = 6.842e-8 Identity = 42/107 (39.25%), Postives = 48/107 (44.86%), Query Frame = 3 Query: 1581 CETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCG 1883 C QCPGN ++ C E CP C L Q P C S C C G + +C+ CPC GQ + PG V DCN CTC S CT K CP CG Sbjct: 2718 CTAQCPGNMVFRSAEQCLEEGGPCPQLC--LAQDPGVECTGSCAPSCNCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVAHLDCNNCTCISGEMVCTSKRCPVACG 2822 HSP 5 Score: 56.9954 bits (136), Expect = 2.000e-6 Identity = 40/132 (30.30%), Postives = 60/132 (45.45%), Query Frame = 3 Query: 4428 TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISR-------------TCIEEKKICDGNYDCGVNDKS----DEENC 4769 + C+ +F+C+ G+C CD+ DC++ DE +C C L Q C + C+ + CDG C D SDE C +R TCI+ +CDGN DC + D++ DE+ C Sbjct: 1441 SPCSLLEFQCNSGECTPRGWRCDQEEDCTDGSDELDCGGPCMLYQVPCAHSPHCVSPGQLCDGVTQCPDGSDEDPDVCEEQSASGGANRTGAPCPEFSCPDGTCIDFLLVCDGNPDCELADETEPSLDEQGC 1572
BLAST of SCO-spondin vs. UniProt/SwissProt
Match: sp|A2VEC9|SSPO_HUMAN (SCO-spondin OS=Homo sapiens OX=9606 GN=SSPO PE=2 SV=2) HSP 1 Score: 427.172 bits (1097), Expect = 1.876e-120 Identity = 420/1500 (28.00%), Postives = 637/1500 (42.47%), Query Frame = 3 Query: 510 CQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGS--WGSQSGSCVSCKANFAGGN------------------DVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVY-ENCTSDTGCVKV------IVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXX-----XXXVASFGNSFK----GFGCPDIANTLSTC-KPDDQILANQQCSKIRLK------------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPG-TVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFDGAKFAF-NDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSCDNTPQSIVDPPAGDN------DICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCIN-GRLNCKTKDNCTNPVCPKNQVYMDSVS--GCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTI--------PLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDC---ITTCTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNCNV--CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 C + G C E ++ + +C+ QC + A S +ECCA G W S +C SC + G+ + TC S+ +YRTFD + Y + GRC+Y+L +D TW++++ + C C +V ++I+AG+V VK G L + L LS+ L++ G V+R D G+I + D+ G GLCG + P +DF A+FGNS++ GC D C P I A+ + +R + F C V+ Y C+ C + + +C ++Y +C+ + I+WR CE CPG +++SDC S CP +C + Q C+ V C C G G C+ CPC Y Q + PG TVRQ CN C C WHC + +CP+ C + G + TFDG ++F GC+Y V+ V + + ++++ + S ++ + L+ T QL DS V ++G+D LP+ I + + VR+ ++ + ++ L+ YI + + + GLCG F N ++DF + SI A + G C + +++ P + + IL+ F C VDKE + C D S C + +A A RR + P N Q P+ GG ++ C+ C C + + C GC CP GQCV S C C +R P G+ C C+C G NC + + CP+ VY C+S + +C S+ + C CP TV +D CV P CPC + + Y PN I C +C R W+ + + C A G PHY T DG F++ G+C Y L+ T+S N+ CG S TC K + + + G + RG T+ ++ + + + F L+S +GL +LWD TRV + L P F+G V GLCG++DGD N+ E + A + +CP P C N R WA CG + +F C H +V ++Y CLYDACGC+ GGDCECLC+++AT+A +CA G V+WR+Q LC + CEGG+ Y+AC CP TC HC CVEGC CP G + G C+ P +CPC + S+ G++++ ++C C C + +++C D C + C + C ++G C+ +CD DC + DE+ C C Q C S+G C+P+ CD +C D +DE S C + C R C+ +CDG+ DC D +DEE+C Sbjct: 94 CCPGWGGAHCTE-----ALAKASPEGHCFAMWQC-QLQAGSANASAGSLEECCARPWGQSWWDGSSQACRSCSSRHLPGSASSPALLQPLAGAVGQLWSQHQRPSATCASWSGFHYRTFDGRHYHFLGRCTYLLAGAADS---TWAVHLTPGDRCPQPGHCQRVTMGPEEVLIQAGNVSVK----GQLVPEGQSWLLHGLSLQWLGDWLVLSGGLGVVVRLD-------RTGSISISVDHELW-----------GQTQGLCGLYNGWPEDDFMEPGGGLAMLAATFGNSWRLPGSESGCLDAVEVAQGCDSPLGLIDADVEPGHLRAEAQDVCHQLLEGPFGQCHAQVSPAEYHEACLFAYCAGAMAGSGQ---EGRQQAVCATFASYVQACARRHIHIRWRKPGFCERLCPGGQLYSDCVSLCPPSCEAVGQGEEESCREECVSGCECPRGLFWNGTLCVPAAHCPCYYCRQRYVPGDTVRQL-CNPCVCRDGRWHCAQALCPAECAVGGDGHYLTFDGRSYSFWGGQGCRYSLVQDYV-----KGQLLILLEHG-----ACDAGSC-----------LHAISVSLEDTHIQLRDSGA-VLVNGQD--VGLPW-IGAEGLSVRRASSAFLLLRWPGAQVLWGLSDPVAYITLDPRHAHQVQGLCGTFTQNQQDDFLTPAGDVETSIAAFASKFQVAGKGRCPSEDSALLSPCTTHSQRHAFAEAACAILHSSVFQEC--HRLVDKEPFYLRCL-AAVCGCDPGSDCLCPVLSAYA-RRCAQEGASPPWRN--QTLCPVMCPGGQEYRECAPACGQHCGKPEDCGEL---GSCVAGCNCPLGLLWDPEGQCVPPSLCPCQLGARRYAP----GSATMKECNRCICQERGLWNCTARHCPSQAFCPRELVYAPGACLLTCDSPSANHSCPAGST--DGCVCPPGTVL-LDERCVPPDLCPCRHSGQWYLPNATIQEDCNVCVCRGRQWHCTGQRRS---GRCQASGAPHYVTFDGLAFTYPGACEYLLVREASGL------FTVSAQNLPCGASGLTCTKALAVRLEGTVVHMLRGRAVTVNGVSVTPPKVYTGPGLSLRRAGLFL-LLSTHLGLTLLWDGGTRVLVQLSPQFRGRVAGLCGDFDGDASNDLRSRQGVLEPTAELAAHSWRLSPLCP---------EPGDLPHPCTMNTHRAGWARARCGAL--LQPLFTLC----HAEVPPQQHYEWCLYDACGCDSGGDCECLCSAIATYADECARHGHHVRWRSQELCSLQCEGGQVYEACGPTCPPTCHEQHPEPGWHCQVVACVEGCFCPEGTLLHGGACLEPASCPCEWGRNSFPPGSVLQ-KDCGNCTCQEGQWHCGGDGGHCEELVPACAEGEALCQENGHCVPHGWLCDNQDDCGDGSDEEGCAAPGCGEGQMTC-SSGHCLPLALLCDRQDDCGDGTDEPSYPCPQGLLACADGR-CLPPALLCDGHPDC--LDAADEESC 1487 HSP 2 Score: 67.0106 bits (162), Expect = 1.884e-9 Identity = 32/80 (40.00%), Postives = 45/80 (56.25%), Query Frame = 3 Query: 4173 PCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 PCP+ C T G P T C GC+CP G+ N C+P + CPC +GQ Y +GA+ ++++C C CV K CT + Sbjct: 2905 PCPRLCLTQG---PGIECTGFCAPGCTCPPGLFLHNASCLPRSQCPCQLHGQLYASGAMARLDSCNNCTCVSGKMACTSE 2981 HSP 3 Score: 59.3066 bits (142), Expect = 4.726e-7 Identity = 35/108 (32.41%), Postives = 55/108 (50.93%), Query Frame = 3 Query: 2766 CQSTCRELNEA-TASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPK-NQVYMDSVSGCNSC 3083 C+ TC E+N T S C GC C P ++++ G CV E C CW+ RP P G+ C++C+C++GR C +C+ C + ++ ++ S C SC Sbjct: 4943 CEQTCLEMNATKTQSNCSSARASGCVCQPGHFRSQAGPCVPEDHCECWHLGRPHLP----GSEWQEACESCLCLSGRPVC--TQHCSPLTCAQGEEMVLEPGSCCPSC 5044 HSP 4 Score: 58.5362 bits (140), Expect = 8.022e-7 Identity = 42/131 (32.06%), Postives = 58/131 (44.27%), Query Frame = 3 Query: 4434 CTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC-NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDE--ISCGCLPH------------QIVCNISRTCIEEKKICDGNYDCG----VNDKSDEENC 4769 C P +FRC G+C CD+ DC++ DE+ C C C C+ + CDG + C D SDE +CG LP + C + TCI + +CDG DCG V +E+ C Sbjct: 1566 CGPFEFRCGSGECTPRGWRCDQEEDCADGSDERGCGGPCAPHHAPCARGPHCVSPEQLCDGVRQCPDGSDEGPDACGGLPALGGPNRTGLPCPEYTCP-NGTCIGFQLVCDGQPDCGRPGQVGPSPEEQGC 1695 HSP 5 Score: 57.7658 bits (138), Expect = 1.408e-6 Identity = 57/201 (28.36%), Postives = 73/201 (36.32%), Query Frame = 3 Query: 2703 LQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRP---------KDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDT-----SSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYIC 3263 LQ C P C G V C+ +C C L + A + C PGC CP G CV + C C S P + E+P GT +T NC CVC G +C D P QV + C T T S+ A+ C C G CV C C ++ R + P C +C Sbjct: 4815 LQPC-PECPPG-QVLSACATSCPCLCWHL-QPGAICVQEPCQPGCGCPGGQL-LHNGTCVPPTACPCTQHSLPWGLTLTLEEQAQELPPGTVLTRNCTRCVCHGGAFSCSLVDCQVPPGETWQQVAPGELGLCEQTCLEMNATKTQSNCSSARASGCVCQPGHFRSQAGPCVPEDHCECWHLGRPHLPGSEWQEACESCLC 5011 HSP 6 Score: 56.6102 bits (135), Expect = 2.710e-6 Identity = 41/133 (30.83%), Postives = 55/133 (41.35%), Query Frame = 3 Query: 1506 CTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIW---SDCASE---CPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD-CNTCTCNSSLWHCTEKICPSTCG 1883 C+ L+ +C S + + C QCP N ++ C E CP C L Q P C C C G + +C+ CPC GQ + G + + D CN CTC S CT + CP CG Sbjct: 2858 CSSLAPGDTTCPGPHSQTRDCNTQPCTAQCPENMLFRSAEQCHQEGGPCPRLC--LTQGPGIECTGFCAPGCTCPPGLFLHNASCLPRSQCPCQLHGQLYASGAMARLDSCNNCTCVSGKMACTSERCPVACG 2988 HSP 7 Score: 55.0694 bits (131), Expect = 8.072e-6 Identity = 59/251 (23.51%), Postives = 86/251 (34.26%), Query Frame = 3 Query: 2712 CIPLCQAGGTVFQI-CSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKD-----------NCTNPV--------------------------------------CPKNQVYMDSVSGCNSCTNYDTCTDTSSIAN----SCGCPNDTVSKIDGTCVQPSDCPCIYMN-------------REYAPNDVITIKCAKYIC 3263 C C+A TVF + C+ C +C +L + C PGC CPP G+CV S C C PS E+ + ++CQ C C+N L C ++ +C+ P CP QV + C C + + CGCP + +GTCV P+ CPC + +E P V+T C + +C Sbjct: 4666 CPESCEAQDTVFTLDCANQCPHSCADLWDRVQC-LQGPCRPGCRCPPGQL-VQDGRCVPISSCRCGLPSANASWELAPAQAVQLDCQNCTCVNESLVCPHQECPVLGPWSAWSSCSAPCGGGTMERHRTCEGGPGVAPCQAQDTEQRQECNLQPCPECPPGQVLSACATSC-PCLCWHLQPGAICVQEPCQPGCGCPGGQLLH-NGTCVPPTACPCTQHSLPWGLTLTLEEQAQELPPGTVLTRNCTRCVC 4912
BLAST of SCO-spondin vs. UniProt/SwissProt
Match: sp|P98167|SSPO_BOVIN (SCO-spondin OS=Bos taurus OX=9913 GN=SSPO PE=2 SV=2) HSP 1 Score: 398.282 bits (1022), Expect = 3.339e-111 Identity = 400/1453 (27.53%), Postives = 607/1453 (41.78%), Query Frame = 3 Query: 645 AVSYDECCADKTG-SW-GSQSGSCVSCKANFAGGNDVN------------------NENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAG--DVVVKSSG---NGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXX-----XXXVASFGNSFK----GFGCPDIANTLSTCK-PDDQILANQQCSKIRLK------------FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPG-TVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFDGAKFAFND-VGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT---GSCDNTPQSIVDPPAGDN------DICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNP--VCPKNQVYMDSVS--GCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNN---DTIPLFPKYDIVIEQLTFFTQ----LVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITT---CTPSQFRC-DDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC 4769 A S +ECCA G SW +S +C SC + G+ + TC ++ +YRTFD + + + GRC+Y+L +D TW++++ G ++ + G +V+++ NG L ++ L LS+ L++ G V+R D +S+ G GLCG + P +DF A+FGNS++ GC D C+ P + ++R + F C V Y C+ C + + + + +C L++Y+ C+ + ++WR CE CPG +++SDCAS CP +C + + C V C C G G C+ CPC + + PG TVRQ CN C C W C + C + C + G + TFDG F+F GC+++ V+ D +I+ + + G+ ++ + L T QL DS V +DG+D LP++ + V + ++ + ++ L+ + YI + + + GLCG F N ++DF + SI A + G+C + + P + ++ IL+GP F C VD+E + C +L A RR + +P N Q P+ GG +Q C+ C C E + D+C GC CP GQCV + C C + P G+ +C CVC L T +C P CP+ VY+ C+S TC S C CP TV ++ CV P CPC + + Y PN I C +C + W+ + C+ C A G PHY T DG +F G+C Y L+ +S N+ CG S TC K + + + G + RG + + + P L+ T L+S +GL +LWD TRV + L P +G V GLCG++DGD N+ E + A +CP P C N R WA CGV+ +FA C H +V ++Y C+YDACGC+ GGDCECLC+++AT+A +CA GI V+WR+Q LCP+ CE G+ Y+AC CP TC C CVEGC CP G + G C+ P CPC + G + G +++ + C C ++++ C +D C+ C + C + G C+ +CD DC + DE+ C VC Q C S G C+P+ CDG +C D DE C C + C C+ ++CDG+ DC D +DEE+C Sbjct: 125 AGSLEECCAQPWGHSWRDGRSQTCHSCSNHSRLGSTPSPAILQPLAGAVAQLWSQRQRPSATCATWSGFHYRTFDGRHFHFLGRCTYLLAGAADA---TWAVHLQPMGHCPQPGHCQLARVMMGPEEVLIRGENVTVNGRLVPEGASQLLPGLSLQWQGDWLVLSGGLGVVVRLDRSSSVSISVDQ------------------ELQGQTQGLCGVYNGQPEDDFLEPGRGLAALAATFGNSWRLPDSELGCLDAVEAAQGCEDPLRGTETGTEAGQLRAEAQDVCHQLLEGPFRECHTQVPPAEYHEACLFAYCAGAPAGSGRAER---LEAVCATLASYAQDCAARRIAVRWRKPGFCERLCPGGQLYSDCASACPPSCSAVGEGSEWSCGEECVSGCECPPGLFWDGALCVPAARCPCYRRRRRYEPGDTVRQL-CNPCECRDGRWLCAQAPCAAECAVGGDGHYVTFDGRSFSFRGRAGCRFILVQ------DFAKRQLLIVLEHGDCDAGS---------------CLHAISVSLGDTLVQLRDSGV-VLVDGQD--VALPWSAAG-GLSVSRASSSFLLLRWPGARILWGVSDPAAYITLDPHHAHQVQGLCGTFTRNQQDDFLTPAGDVETSITAFASKFQVAGGGTCSLEACTPLSPCSTHTERQVFAEVACAILHGPTFQEC--HGLVDREAFHLRCLAAVCGCTPGRDCLCPVL--AAYARRCAQEGALPSWRN--QTFCPVLCPGGQEYQECAPACDRNCGEPEDCGEL---DNCVAGCNCPLGLLWDPEGQCVPPNLCPCQLGAHRYAP----GSATMKDCNHCVCQERGLWNCTAHHCAPPRTFCPRELVYVPGACLLTCDSLDADRTCPPGS--PGGCVCPPGTV-LLEERCVPPELCPCRHGGQWYLPNAAIQEDCNLCVCQGQQWHCTGQR---CDGRCRASGAPHYVTFDGLALTFPGACEYLLVREASGQ------FMVSAQNLPCGASGLTCTKALTVRLQGTVVHMLRGRAVMVNGVSVTPPKVYSGPGLSLHTAGLFLLLSTRLGLTLLWDGGTRVPVQLSPQLRGRVAGLCGDFDGDASNDLRSRQGVLEPTAELAAHSWRLGPLCP---------EPGDLPHPCAVNAHRAGWARARCGVV--LQPLFARC----HVEVPPQQHYEQCVYDACGCDSGGDCECLCSAIATYADECARHGIHVRWRSQELCPLQCERGQVYEACGPTCPATCHDHRPEPGWPCRAVACVEGCFCPEGTLLHGGVCLEPAACPCEWGGSFFPPGTVLQKDCGNNCTCRESQWLCGDDGGRCVEPGPGCAEGETPCRESGHCVPHGWLCDNQDDCGDGSDEEGCATRVCGEGQVSCCS-GRCLPLVLLCDGQDDCGDGMDEQGCPCPQDSLTCADGH-CLPPARLCDGHPDC--PDGADEESC 1483 HSP 2 Score: 62.7734 bits (151), Expect = 3.471e-8 Identity = 29/80 (36.25%), Postives = 41/80 (51.25%), Query Frame = 3 Query: 4173 PCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 PCP C G P T C GC+CP G+ N C+PP+ CPC GQ Y GA+ ++++C C C+ + C + Sbjct: 2883 PCPGLCLARG---PGVECTGVCTAGCACPTGLFLHNSSCLPPSQCPCQLRGQLYAPGAVARLDSCSNCTCISGEMVCASE 2959 HSP 3 Score: 59.6918 bits (143), Expect = 3.674e-7 Identity = 35/108 (32.41%), Postives = 47/108 (43.52%), Query Frame = 3 Query: 1581 CETQCPGNKIWSDC------ASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD-CNTCTCNSSLWHCTEKICPSTCG 1883 C QCPG+ ++ CP C L + P C C C +G + +C+ P CPC GQ + PG V + D C+ CTC S C + CP CG Sbjct: 2861 CTAQCPGDMVFRSAEQCRWEGGPCPGLC--LARGPGVECTGVCTAGCACPTGLFLHNSSCLPPSQCPCQLRGQLYAPGAVARLDSCSNCTCISGEMVCASEPCPVACG 2966
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267FQZ0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032551g1 PE=4 SV=1) HSP 1 Score: 1132.86 bits (2929), Expect = 0.000e+0 Identity = 665/1654 (40.21%), Postives = 922/1654 (55.74%), Query Frame = 3 Query: 204 SCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYV---SSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNE--NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDV-----------VVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGD-VTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCK-PDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKI-CPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPA------GDNDICSTILNGPEFSGCMNSSKVD-KEFYATYCRREQS----SNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECP-PNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNN-DTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV-NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQ--AKFNCTEDYSDCI---TTCTPSQFRCD-DGKCIESSKICDKVTDCSNSEDEKNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKC-QMTEYKCNNSQI----CILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 SC + + ME +D+ SVPI + +++ LS+Q Y+ + P C F + + CCVGY+ C VC+ D GGC NGG C+ PN C+C NY G CDED I +P++ YCY D C KK+ F V++ CC+D TGS+G Q G C+SCK + N+ E F TC SYG + YRTFD + YQGRC+Y+L A S + W I NC C K I + D+ V K SG T +N L S N + + V R+ + L++ + +KLKWDN N++F+++ S++ + GLCG+ D + +NDF + N A+FGN +K C DIAN C+ P+DQ A C I F C ++ CVQ++C + T+ + S VLC+HLSAYS CS +G CI WR T+C CP WS+CAS CP TC N Q SA C++S V RC CGSG V Y TCI+P CPC Y+ E+ PG+ DC+TC C + W CT CP C I G+ TFDGAK FN +GC Y +EP D R V V ++ + E +L +S ++N PK++TVK G I LK+ + G ++ +V IDG D T LP+ I VR+LT+ Y VK+ +H +YFDG +++ +K + L + GLCG FD +END K + + V +A+SY++GSC T + P + G +D +LN F C +S KVD +Y +C++E++ N +L YC+A ++AA AC+ G Q + T + L C P C +GG F IC+RTC+S+ + ++ S C+ CFPGC+C N Y+ G CV +SECTC + +G I+ C C C NG C VCPK+QVY++++ C C NYD C S + CGCP D V K DG+CV CPCIY+ EY P ++ I C Y C +R W ++E+ CEAVC+AYGDPHY T D + +SFQGSC Y LM + +++ +PPI +S NI CG TC KFV++ +G TY+L RG D +P+ Y + E+ T FTQLVSD +GLR+LWDRSTRVY+F+KP F V GLCGN+DG+ N++ + + I+FA +F D C + +P +MC AN R+ +AE +CG +K GVF++C + + QDV +Y NC+YDACGC+ GGDCEC+CT L+ FAT CA G+ V+WR Q+ CPIMC GG+ Y C PC +TC +G HC + C+EGC+CPNG ++ + EC+P CPCFY G+ Y G++I NCT D S C TCT +Q+ C+ G CI S+ CD DC +DEKNCN C F+CI+ C+ M+ KCDG C D SDE++CGC P +I CN + C+ ++K CDG YDCG+ D +DE++CKKC +++E+KC + CI N CDG DDCGDGSDE GC+C+C E + ++C C+CIN T+ CDG DC +G DE++C Sbjct: 66 SCGNMVEMTMEGLTIDKSSVPILTDAALANRTTHEKRQLSLQTYIRDFAYSKATPNCTFYKLTNIEKTQCCVGYDG-SNCQTVVCFVDRGGCLNGGKCVKPNVCQCATNYLGTNCDEDLNAI--KNNPNEQYCYSDDACLT-KKTGFGSSRVAFGRCCSDGTGSFGYQKG-CMSCKTSTVARNNTGVEVVPFDTCVSYGPNSYRTFDGLTFEYQGRCTYLL-AGSTNPTMKWWCTITLLNCDKFETCRKQITMRFDDINQYVALGYNVQVFKLSGTSGATTLTANVTLEENSEPNEWNGV-------KVERKGVNVYLTVRSIGVKLKWDNENSVFVTVDRSLHKNRGLKGLCGDYDGDATNDFGSAPNPAAFGNQWKSESCQDIANVPIHCESPEDQAAAEGDCRPINNVFFQCHSKISPTQVVKRCVQDMCILKKKGETSDS--AKSSVLCSHLSAYSYQCSLEGICIDWRGSTMCPQSCPALMTWSECASACPTTCANYDQLKSATCESSMVPRCKCGSGYVHYKSTCIRPNECPCTYNSVEYMPGSNLVVDCSTCNCANGRWQCTYNPNCPKMCSITGSHIVTFDGAKMDFNPMGCAYTLLEPVSGANDSRLQVQVTMEPSPEYLLAVTADSQFVNLPKMITVKYRGVTIQLKSVTLAAG-TRPSVIIDGVDFTNSLPYENVRSQILVRQLTSFYAMVKIGNHLSVYFDGYRTVELKGAKLLSQKIEGLCGNFDSKTENDLHTKGSDMIAPPVQVAQSYVSGSCSKTAAELPSPGSTSQSLSGVDDCTQVLLNSDSFKACRDSKKVDILGYYYRFCQKEKACGGIPNIGEL-YCTAAMSAARACKTAGFQIDFS-TDSRLSKCAPSCMSGGGSFTICARTCRSS--CSDLSSTSICELGCFPGCDCQYDNQYRDAYGTCVRQSECTCLDRYNRNGKIYRSGAVISRQCTNCTCTNGDFACGNSSCADKVVCPKDQVYLETMPACQKCINYDKCQSNQSESGRCGCPGDLVKKADGSCVAKPHCPCIYLGTEYLPGHILKIGCKTYKCVSRTWTKIAEDATKCEAVCVAYGDPHYSTFDRKAYSFQGSCQYILMKSDANAALGLPPIEVSAQNIPCGSQQVTCTKFVRVKVGSDTYVLVRGREPDQLPVAMNYGMRREKFTLFTQLVSDQLGLRILWDRSTRVYVFVKPHFNSKVSGLCGNFDGNADNDFTIKNLPINNPIEFASNFRTGGDSC-ASEESSASKSPQSWIEMCTANSRRRDYAESVCGAIKSKTGVFSACQQVLEQDVIENFYENCMYDACGCDTGGDCECVCTVLSAFATTCAESGVSVQWRFQDQCPIMCTGGRVYQPCMPPCAKTCHNVGLEPEPHCVNSQCIEGCACPNGYVEHDNECIPSDNCPCFYQGKEYPKGSVIHNTTSCSTCSCTKGGVMNCTSDSSVCRPTKPTCTENQYACNVTGMCISYSQFCDGTLDCLYGDDEKNCNTTCSPSYFRCITTQQCVAMSLKCDGIPQCTDGSDELNCGCNPTEIQCNKTGKCLPKQKRCDGVYDCGLPDTTDEDDCKKCDKVSEFKCGAADTTETKCIPRVNHCDGHDDCGDGSDEIGCKCLCMEKDTVIC----------DQCKCINVTQLCDGNRDCEDGTDEQSC 1688
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267FNX5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000138g1 PE=4 SV=1) HSP 1 Score: 1130.55 bits (2923), Expect = 0.000e+0 Identity = 665/1654 (40.21%), Postives = 921/1654 (55.68%), Query Frame = 3 Query: 204 SCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYV---SSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNE--NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDV-----------VVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGD-VTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCK-PDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCT-EKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPA------GDNDICSTILNGPEFSGCMNSSKVD-KEFYATYCRREQS----SNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECP-PNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNN-DTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV-NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQ--AKFNCTEDYSDCI---TTCTPSQFRCD-DGKCIESSKICDKVTDCSNSEDEKNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKC-QMTEYKCNNSQI----CILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 SC + + ME +DE SVPI + + +++ LS+Q Y+ + P C F + + CCVGY+ C VC+ D GGC NGG C+ PN C+C NY G CDED I +P++ YCY D C KK+ F V++ CC+D TGS+G Q G C+SCK + N+ E F TC SYG + YRTFD + YQGRC+Y+L A S + W I NC C K I + D+ V K SG T +N L S N + + V R+ + L++ + +KLKWDN N++F+++ S++ + GLCG+ D + +NDF + N A+FGN +K C DIAN C+ P+DQ A C I F C ++ CVQ++C + T+ + S VLC+HLSAYS CS +G CI WR T+C CP WS+CAS CP TC N Q SA C++S V RC CGSG V Y TCI+P CPC Y+ E+ PG+ DC+TC C + W CT CP C I G+ TFDGAK FN +GC Y +EP D R V V ++ + E +L +S ++N PK++TVK G I LK+ + G ++ +V IDG D T LP+ I VR+LT+ Y VK+ +H +YFDG +++ +K + L + GLCG FD +END K + + V +A+SY++GSC T + P + G +D +LN F C +S KVD +Y +C++E++ N +L YC+A ++AA AC+ G Q + T + L C P C +GG F IC+RTC+S+ + ++ S C+ CFPGC+C N Y+ G CV +SECTC + +G I+ C C C NG C VCPK+QVY++++ C C NYD C S + CGCP D V K DG+CV CPCIY+ EY P ++ I C Y C +R W ++E+ CEAVC+AYGDPHY T D + +SFQGSC Y LM + +++ +PPI +S NI CG TC KFV++ +G TY+L RG D +P+ Y + E+ T FTQLVSD +GLR+LWDRSTRVY+F+KP F V GLCGN+DG+ N++ + + I+FA +F D + +P +MC AN R+ +AE +CG +K GVF++C + + QDV +Y NC+YDACGC+ GGDCEC+CT L+ FAT CA G+ V+WR Q+ CPIMC GG+ Y C PC +TC +G HC + C+EGC+CPNG ++ + EC+P CPCFY G+ Y G++I NCT D S C TCT +Q+ C+ G CI S+ CD DC +DEKNCN C F+CI+ C+ M+ KCDG C D SDE++CGC P +I CN + C+ ++K CDG YDCG+ D +DE +CKKC +++E+KC + CI N CDG DDCGDGSDE GC+C+C E + ++C C+CIN T+ CDG DC +G DE++C Sbjct: 66 SCGNMVEMTMEGLTIDESSVPILTDAALANRTMHEKRQLSLQTYIRDFAYSKATPNCTFYKLTNIEKTQCCVGYDG-SNCQTVVCFVDRGGCLNGGKCVKPNVCQCATNYLGTNCDEDLNAI--KNNPNEQYCYSDDACLT-KKTGFGSSRVAFGRCCSDGTGSFGYQKG-CMSCKTSTVARNNTGVEVVPFDTCVSYGPNSYRTFDGLTFEYQGRCTYLL-AGSTNPTMKWWCTITLLNCDKFETCRKQITMRFDDINQYVALGYNVQVFKLSGTTGATTLTANVTLEENSEPNEWNGV-------KVERKGVNVYLTVRSIGVKLKWDNENSVFVTVDRSLHKNRGLKGLCGDYDGDATNDFGSAPNPAAFGNQWKSESCQDIANVPIHCESPEDQAAAEGDCRPINNVFFQCHSKISPTQVVKRCVQDMCILKKKGETSDS--AKSSVLCSHLSAYSYQCSLEGICIDWRGSTMCPQSCPALMTWSECASACPTTCANYDQLKSATCESSMVPRCKCGSGYVHYKSTCIRPNECPCTYNSVEYMPGSNLVVDCSTCNCANGRWQCTYNPNCPKMCSITGSHIVTFDGAKMDFNPMGCAYTLLEPVSGANDSRLQVQVTMEPSPEYLLAVTADSQFVNLPKMITVKYRGVTIQLKSVTLAAG-TRPSVIIDGVDFTNSLPYENVRSQILVRQLTSFYAMVKIGNHLSVYFDGYRTVELKGAKLLSQKIEGLCGNFDSKTENDLHTKGSDMIAPPVQVAQSYVSGSCSKTAAELPSPGSTSQSLSGVDDCTQVLLNSDSFKACRDSKKVDILGYYYRFCQKEKACGGIPNIGEL-YCTAAMSAARACKTAGFQIDFS-TDSRLSKCAPSCMSGGGSFTICARTCRSS--CSDLSSTSICELGCFPGCDCQYDNQYRDAYGTCVRQSECTCLDRYNRNGKIYRSGAVISRQCTNCTCTNGDFACGNSSCADKVVCPKDQVYLETMPACQKCINYDKCQSNQSESGRCGCPGDLVKKADGSCVAKPHCPCIYLGTEYLPGHILKIGCKTYKCVSRTWTKIAEDATKCEAVCVAYGDPHYSTFDRKAYSFQGSCQYILMKSDANAALGLPPIEVSAQNIPCGSQQVTCTKFVRVKVGSDTYVLVRGREPDQLPVAMNYGMRREKFTLFTQLVSDQLGLRILWDRSTRVYVFVKPHFNSKVSGLCGNFDGNADNDFTIKNLPINNPIEFASNFRTGGDS-CASEESSASKSPQSWIEMCTANSRRRDYAESVCGAIKSKTGVFSACQQVLEQDVIENFYENCMYDACGCDTGGDCECVCTVLSAFATTCAESGVSVQWRFQDQCPIMCTGGRVYQPCMPPCAKTCHNVGLEPEPHCVNSQCIEGCACPNGYVEHDNECIPSDNCPCFYQGKEYPKGSVIHNTTSCSTCSCTKGGVMNCTSDSSVCRPTKPTCTENQYACNVTGMCISYSQFCDGTLDCLYGDDEKNCNTTCSPSYFRCITTQQCVAMSLKCDGIPQCTDGSDELNCGCNPTEIQCNKTGKCLPKQKRCDGVYDCGLPDTTDENDCKKCDEVSEFKCGAADTTETKCIPRVNHCDGHDDCGDGSDEIGCKCLCMEKDTVIC----------DQCKCINVTQLCDGNRDCEDGTDEQSC 1688
BLAST of SCO-spondin vs. TrEMBL
Match: A0A1I8IM20 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 1046.19 bits (2704), Expect = 0.000e+0 Identity = 633/1678 (37.72%), Postives = 919/1678 (54.77%), Query Frame = 3 Query: 150 SPNNCIYDYNEDVDTVTESCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYV---SSGMPEPQCNFTLQNIVSEENCCVGY--ENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQL-YCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNEN-------FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDV--VVKSSGNGILYTTDSNNPLTPLSVTNT-----DSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXG-DVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILAN--QQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPG-NKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKI-CPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTP-----QSIVDPPAGDNDICSTILNGPEFSGCMNSSKVD-KEFYATYCRREQSS---NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECP-PNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN-NDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQD-VNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNG-AIIKMENCKKCQC-VQAKFNCTEDYSDCITTCT---PSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC----NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQI--VCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKC-QMTEYKCNNS----QICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDE 5021 S N C YDY E +TV SC + + + + SVP++ +P + Q+S ++ Y+ ++G P PQC F+ + CC GY EN C VC+ +GGC NGG C+ P++C+CQ + G CDED + + Q ++L YC+ C K + F K + +CC+ S+G SG C +C ++ G + F TC S+G + TFD Y ++GRCSY+L + S W + + NC + C K + D+ V + +Y + L V ++ D A G ++ R D+ I+ S G ++L+WD+ ++ +++ +++ D+ GLCGN D + N+F F N ++FGN++K CPD AN C PD + A+ +C +I F C D + +F CVQ+ C + T S+V+C HLSAY+ CS G CI WRS LC T C N W +CASECP TC N Q S CQ + RC C V + C++ CPC Y+G+E P G C +C C W C ++ CP TC + G+ F TFDGA+ FN C+YV +EP+ N TD R NV + + TE++L T+ N N PK V + I L ATS G S+ +VFI+ ED T LPF+ D ++ V+++T++ T VK+ SH +YFDG ++I IK ++ +++N+ GLCG +DG++END +++ + S+ +A+SYITG C ++P + D +G + +L G F C S+KVD Y CRRE +S Q +CS+++ AAI C+ G Y+V F + + + LC GT F +CSRTC+STC +L + C++ CFPGC+C + Y+ G CV +SECTC + P AG + +C C C NG C+ + VCPKNQV+MD V C C +Y+ C ++ + CGCP V K DGTCV + CPC + +EY P + + C +++C ++ W + ++ CEAVC A GDPHY T D + F+FQG C YTL+ GD+S +P IS N+ CG TC KFV+IIIG + Y L RG D P F Y + +E LT +TQ+VSD +GLR+LWDR TRV ++L+ ++ V GLCGNYDG+ N++ + ++AE + A+ CP E N+ + +D+C NP RK+W+E CGV+K+ NG F C+ A+ D V +Y NC++DACGC+ GGDCEC+C+++A FA C+ +G+ VKWR+ + CP+MC+GGK Y C PCPQTC +G HC C EGC CP G +D NGECVP + CPC NG Y +G + C++C C AKF+C C + F+C +G CI ++CD+ DC + DEK+C C + FQC +G CIP +K+CDG +C D SDE C + +C +S CI +++CDG DCG NDKSDE +CK+C Q E++C ++ C+ L +CDG DDCGDGSDE+ C+CIC + +M LC C+CI+ K CD V DC +G DE Sbjct: 218 SENQCDYDYLEANETVA-SCGD-LSFTLAGFTISGDSVPVSPACANPRASIGDQRSACIRKYILDLATGKPTPQCKFSKPQKTTRRGCCPGYGGEN---CQQVVCFPKDGGCQNGGRCVKPDKCECQSGFQGAMCDED---LAELQKDTRLRYCFSDASC-KVKNTKFGRKTTPFSDCCSAGGNSFG-YSGVCTACTSS-TGTRSLGTPTGHSEIVPFDTCSSFGPNSISTFDGLTYEFEGRCSYLLAGSINPSR-RWFVKVAMVNCDTFKSCQKTLRFRLDDLHEFVAVGQSLQVYQISGLDGAQMLKVNDSFQGLFDDARDYSGV-RFIRRGDSIIMTSRSLG-LRLRWDSIGSVQLTLDRPVHSNQDLQGLCGNYDGDKKNEFGEFANPSAFGNAWKAQDCPDSANVPVHC-PDTKTTASAEMECRQIDSIFFPCHDRIPPQEFFQRCVQDACLAIRRGEPT------SEVVCRHLSAYAGLCSLGGICIDWRSSNLCPTNCSEPNMYWKECASECPTTCANYDQVKSELCQVTTSPRCECEPDYVHHKGKCVRKTDCPCRYNGKEFPAGGTFNTQCESCQCIQGRWSCYKRASCPKTCLVTGSSFRTFDGAQLDFNGRTCEYVLLEPAPNVTDSRLNVTITQRVRTENVLITDPNQ-LANLPKRVHISYAAYRIELVATSLNYG-SRASVFINDEDVTNSLPFSNQDDHLLVKQITSLTTMVKIGSHITVYFDGFRNIEIKANERVRSNVKGLCGNYDGDTENDLHPRNSDMFGSVTELAESYITGVCTDSPIPLRQAAFADQVSGSDHCQLALLTGDSFKACRESNKVDLNGHYLRSCRREVASGGVQGQQFLFCSSLVQAAIECKNQG--YDVDFYRD---SVLSLC---GT-FSLCSRTCRSTCSDLE--STEICEEGCFPGCDCQYDSQYRDHHGTCVRKSECTCRDRFNPDSAIYKAGDVLQRSCTNCTCSNGAFVCEDEACEDKVVCPKNQVFMDLVPSCQRCEDYNNCPRSAMLQKRCGCPGGQVKKSDGTCVPANQCPCKLLKKEYPPMHKLKMACKRFVCKDQTWKQIGKDR-KCEAVCKAQGDPHYFTFDKKKFNFQGKCQYTLVSFDGDASRGLPKFHISAQNVPCGSKQVTCTKFVRIIIGDERYTLVRGQEKDEFPAPFASYGVKLEHLTLYTQVVSDVLGLRILWDRKTRVLIYLRKDYRRQVSGLCGNYDGNAGNDFTFNNLPMASVDEWAEKYRTGAESCPAEDGSRNS---NGWRDICEQNPGRKTWSELTCGVIKNPNGPFKDCLLALDDDTVQTFYENCIFDACGCDSGGDCECVCSAMAGFADHCSDVGVHVKWRSMDNCPLMCDGGKRYMPCMEPCPQTCRNLGDEPAPHCQNAACTEGCGCPEGTVDQNGECVPISVCPCQRNGTEYQHGQSYFDEATCRECTCGANAKFSCQVKLDKCKVKPKCEDETNFQCTNGACIPREQVCDRKFDCLDRSDEKDCTPVNTTCSVAFFQCQRDGNCIPESKRCDGKAHCSDGSDEFDCQKCTDSLYYLCPVSGECIHRRQLCDGRIDCGFNDKSDESDCKECDQGAEFRCGSTIDKPGPCVPLRKRCDGHDDCGDGSDEYNCKCICTDDDMFLC----------DTCKCISSVKFCDKVRDCEDGTDE 1847
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267FR15 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011629g1 PE=4 SV=1) HSP 1 Score: 989.949 bits (2558), Expect = 0.000e+0 Identity = 562/1410 (39.86%), Postives = 781/1410 (55.39%), Query Frame = 3 Query: 204 SCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYV---SSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNE--NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDV-----------VVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGD-VTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCK-PDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKI-CPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPA------GDNDICSTILNGPEFSGCMNSSKVD-KEFYATYCRREQS----SNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECP-PNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNN-DTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV-NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSY 4334 SC + + ME +D+ SVPI + +++ LS+Q Y+ + P C F + + CCVGY+ C VC+ D GGC NGG C+ PN C+C NY G CDED I +P++ YCY D C KK+ F V++ CC+D TGS+G Q G C+SCK + N+ E F TC SYG + YRTFD + YQGRC+Y+L A S + W I NC C K I + D+ V K SG T +N L S N + + V R+ + L++ + +KLKWDN N++F+++ S++ + GLCG+ D + +NDF + N A+FGN +K C DIAN C+ P+DQ A C I F C ++ CVQ++C + T+ + S VLC+HLSAYS CS +G CI WR T+C CP WS+CAS CP TC N Q SA C++S V RC CGSG V Y TCI+P CPC Y+ E+ PG+ DC+TC C + W CT CP C I G+ TFDGAK FN +GC Y +EP D R V V ++ + E +L +S ++N PK++TVK G I LK+ + G ++ +V IDG D T LP+ I VR+LT+ Y VK+ +H +YFDG +++ +K + L + GLCG FD +END K + + V +A+SY++GSC T + P + G +D +LN F C +S KVD +Y +C++E++ N +L YC+A ++AA AC+ G Q + T + L C P C +GG F IC+RTC+S+ + ++ S C+ CFPGC+C N Y+ G CV +SECTC + +G I+ C C C NG C VCPK+QVY+D++ C C NYD C S + CGCP D V K DG+CV CPCIY+ EY P ++ I C Y C +R W ++E+ CEAVC+AYGDPHY T D + +SFQGSC Y LM + +++ +PPI +S NI CG TC KFV++ +G TY+L RG D +P+ Y + E+ T FTQLVSD +GLR+LWDRSTRVY+F+KP F V GLCGN+DG+ N++ + + I+FA +F D + +P +MC AN R+ +AE +CG +K GVF++C + + QDV +Y NC+YDACGC+ GGDCEC+CT L+ FAT CA G+ V+WR Q+ CPIMC GG+ Y C PC +TC +G HC + C+EGC+CPNG ++ + EC+P CPCFY G+ Y Sbjct: 66 SCGNMVEMTMEGLTIDKSSVPILTDAALANRTTHEKRQLSLQTYIRDFAYSKATPNCTFYKLTNIEKTQCCVGYDG-SNCQTVVCFVDRGGCLNGGKCVKPNVCQCATNYLGTNCDEDLNAI--KNNPNEQYCYSDDACLT-KKTGFGSSRVAFGRCCSDGTGSFGYQKG-CMSCKTSTVAKNNTGVEVVPFDTCVSYGPNSYRTFDGLTFEYQGRCTYLL-AGSTNPTMKWWCTITLLNCDKFETCRKQITMRFDDINQYVALGYNVQVFKLSGTTGATTLTANVTLEENSEPNEWNGV-------KVERKGVNVYLTVRSIGVKLKWDNENSVFVTVDRSLHKNRGLKGLCGDYDGDATNDFGSAPNPAAFGNQWKSESCQDIANVPIHCESPEDQAAAEGDCRPINNVFFQCHSKISPTQVVKRCVQDMCILKKKGETSDS--AKSSVLCSHLSAYSYQCSLEGICIDWRGSTMCPQSCPALMTWSECASACPTTCANYDQLKSATCESSMVPRCKCGSGYVHYKSTCIRPNECPCTYNSVEYMPGSNLVVDCSTCNCANGRWQCTYNPNCPKMCSITGSHIVTFDGAKMDFNPMGCAYTLLEPVSGANDSRLQVQVTMEPSPEYLLAVTADSQFVNLPKMITVKYRGVTIQLKSVTLAAG-TRPSVIIDGVDFTNSLPYENVRSQILVRQLTSFYAMVKIGNHLSVYFDGYRTVELKGAKLLSQKIEGLCGNFDSKTENDLHTKGSDMIAPPVQVAQSYVSGSCSKTAAELPSPGSTSQSLSGVDDCTQVLLNSDSFKACRDSKKVDILGYYYRFCQKEKACGGIPNIGEL-YCTAAMSAARACKTAGFQIDFS-TDSRLSKCAPSCMSGGGSFTICARTCRSS--CSDLSSTSICELGCFPGCDCQYDNQYRDAYGTCVRQSECTCLDRYNRNGKIYRSGAVISRQCTNCTCANGDFACGNSSCADKVVCPKDQVYLDTMPACQKCINYDKCQSNQSESGRCGCPGDLVKKADGSCVAKPHCPCIYLGTEYLPGHILKIGCKTYKCVSRTWTKIAEDATKCEAVCVAYGDPHYSTFDRKAYSFQGSCQYILMKSDANAALGLPPIEVSAQNIPCGSQQVTCTKFVRVKVGSDTYVLVRGREPDQLPVAMNYGMRREKFTLFTQLVSDQLGLRILWDRSTRVYVFVKPHFNSKVSGLCGNFDGNADNDFTIKNLPINNPIEFASNFRTGGDS-CASEESSASKSPQSWIEMCTANSRRRDYAESVCGAIKSKTGVFSACQQVLEQDVIENFYENCMYDACGCDTGGDCECVCTVLSAFATTCAESGVSVQWRFQDQCPIMCTGGRVYQPCMPPCAKTCHNVGLEPEPHCVNSQCIEGCACPNGYVEHDNECIPSDNCPCFYQGKEY 1454 HSP 2 Score: 114.775 bits (286), Expect = 1.656e-21 Identity = 98/400 (24.50%), Postives = 157/400 (39.25%), Query Frame = 3 Query: 3318 CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKY----------------------DIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGN--VQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNA-----NPDRKSWAEQMCGVMKDTNGVFASCIEAMH--QDVNRYYSNCLYDACGCNRGGDCE------CLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 C++YG Y+T DG F +QG C Y L S+N ++ + C TC K + + ++ G N + + +E+ L SIG+++ WD V++ + S N ++GLCG+YDGD N++ P + N QD+ N +P+ ++ AE C + N VF C + Q V R C+ D C + G+ LC+ L+ ++ QC+ GI + WR +CP C + CAS CP TC + C ++ V C C +G + C+ P CPC YN Y+ G+ + ++ C C C ++ CT Sbjct: 255 CVSYGPNSYRTFDGLTFEYQGRCTYLL----AGSTNPTMKWWCTITLLNCD-KFETCRKQITMRFDDINQYVALGYNVQVFKLSGTTGATTLTANVTLEENSEPNEWNGVKVERKGVNVYLTVRSIGVKLKWDNENSVFVTVDRSLHKNRGLKGLCGDYDGDATNDFGSA---------------------PNPAAFGNQWKSESCQDIANVPIHCESPEDQAAAEGDC---RPINNVFFQCHSKISPTQVVKR----CVQDMCILKKKGETSDSAKSSVLCSHLSAYSYQCSLEGICIDWRGSTMCPQSCPALMTWSECASACPTTCANYDQLKSATCESSM-VPRCKCGSGYVHYKSTCIRPNECPCTYNSVEYMPGSNLVVD-CSTCNCANGRWQCT 619 HSP 3 Score: 92.8189 bits (229), Expect = 6.769e-15 Identity = 90/372 (24.19%), Postives = 155/372 (41.67%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWS-INIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGN---IKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXX----XXXXXXXXXVASFGNSFKGFG------------CPDIANTLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDT---YFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSK-VGRCICGSGEVEYGYTCIKPESCPCLYDGQEHP 1790 C +YG +Y TFD + Y++QG C YIL +A+ I + +N + C K + ++ G S +L + L P Y +R++ F L + + +++ WD + +++ + + V+GLCGN D N + +N F ++F+ G P + T + A C I+ K F C ++ D ++ C+ + C D + +CT LSA++ +C++ G +QWR C C G +++ C C TC N+ P +C NS+ + C C +G VE+ CI ++CPC Y G+E+P Sbjct: 1113 AVCVAYGDPHYSTFDRKAYSFQGSCQYILMKSDANAALGLPPIEVSAQNIPCGSQQVTCTKFVRVKVG------SDTYVLVRGREPDQL--------------PVAMNYGMRREKFTLFTQLVSDQLGLRILWDRSTRVYVFVKPHFNS-KVSGLCGNFDGNADNDFTIKNLPINNPIEFASNFRTGGDSCASEESSASKSPQSWIEMCTANSRRRDYAESVCGAIKSKTGVFSACQQVLEQDVIENFYENCMYDACG--------CDTGGDCECVCTVLSAFATTCAESGVSVQWRFQDQCPIMCTGGRVYQPCMPPCAKTCHNVGLEPEPHCVNSQCIEGCACPNGYVEHDNECIPSDNCPCFYQGKEYP 1455 HSP 4 Score: 90.8929 bits (224), Expect = 2.868e-14 Identity = 145/604 (24.01%), Postives = 233/604 (38.58%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGC-NSCTNYD---TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQG-SCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCA-------------KFVQIIIGGQTYLLSRGNNDTIPL--------FP----KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNE-YMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV-NRYYSNCLYD-ACGC--NRGGDCECLCTSLATFATQCAFLGIPVKW----RTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCP--NGMIDLNGECVPPTTCPCF----YNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEIS-CGCLPHQIVCNISRTCIEE 4706 CP + + S C +C NYD + T SS+ C C + V TC++P++CPC Y + EY P + + C+ C+N W NC +C G H T DG F C YTL++ ++++ + +++ + + LA A K+ + I ++ L+ G ++ + P + I++ QLT F +V L V +D V L ++GLCGN+D +N+ + G+ +Q A+S+++ + C + + E S Q + S + V+ +++ F +C ++ D+ YY C + ACG N G E CT+ + A C G + + R P GG + CA C +C + S + C GC C N D G CV + C C NG+ Y +GA+I + C C C F C +S DKV VC Q + AC +KC C+ N E CGC P +V +C+ + Sbjct: 532 CPALMTWSECASACPTTCANYDQLKSATCESSMVPRCKCGSGYV-HYKSTCIRPNECPCTYNSVEYMPGSNLVVDCSTCNCANGRWQCTYN--PNCPKMCSITGS-HIVTFDGAKMDFNPMGCAYTLLEPVSGANDSRLQVQVTM-EPSPEYLLAVTADSQFVNLPKMITVKYRGVTIQLKSVTLAAGTRPSVIIDGVDFTNSLPYENVRSQILVRQLTSFYAMVKIGNHLSVYFDGYRTVELKGAKLLSQKIEGLCGNFDSKTENDLHTKGSDMIAPPVQVAQSYVSGS--CS-KTAAELPSPGSTSQSL--------SGVDDCTQVLLNSDS-FKACRDSKKVDILGYYYRFCQKEKACGGIPNIG---ELYCTAAMSAARACKTAGFQIDFSTDSRLSKCAPSCMSGGGSFTICARTCRSSCSDLSSTSICELG---CFPGCDCQYDNQYRDAYGTCVRQSECTCLDRYNRNGKIYRSGAVISRQ-CTNCTCANGDFACG------------------------NSSCADKV-------------VCPKDQVYLDTMPAC----QKCINYDKCQSNQSESGRCGC-PGDLVKKADGSCVAK 1069
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267FD04 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009912g1 PE=4 SV=1) HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0 Identity = 526/1417 (37.12%), Postives = 774/1417 (54.62%), Query Frame = 3 Query: 138 KSVTSPNNCIYDYNEDVDTVTESCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYV---SSGMPEPQCNFTLQNIVSEENCCVGY--ENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQL-YCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNEN-------FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDV--VVKSSGNGILYTTDSNNPLTPLSVTNT-----DSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXG-DVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILAN--QQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPG-NKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKI-CPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTP-----QSIVDPPAGDNDICSTILNGPEFSGCMNSSKVD-KEFYATYCRREQSS---NKDQLSYCSAILNAAIACRRFGIQYEVPF-TSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECP-PNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN-NDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQD-VNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI 4271 ++ S N C YDY E +TV SC + + + + SVP++ +P + Q+S ++ Y+ ++G P PQC F+ + CC GY EN C VC+ +GGC NGG C+ P++C+CQ + G CDED + + Q ++L YC+ C K + F K + +CC+ S+G SG C +C ++ G + F TC S+G + TFD Y ++GRCSY+L + S W + + NC + C K + D+ V + +Y + L V ++ D A G ++ R D+ I+ S G ++L+WD+ ++ +++ +++ D+ GLCGN D + N+F F N ++FGN++K CPD AN C PD + A+ +C +I F C D + +F CVQ+ C + T S+V+C HLSAY+ CS G CI WRS LC T C N W +CASECP TC N Q S CQ + RC C V + C++ CPC Y+G+E P G C +C C W C ++ CP TC + G+ F TFDGA+ FN C+YV +EP+ N TD R NV + + TE++L T+ N N PK V + I L ATS G S+ +VFI+ ED T LPF+ D ++ V+++T++ T VK+ SH +YFDG ++I IK ++ +++N+ GLCG +DG++END +++ + S+ +A+SYITG C ++P + D +G + +L G F C S+KVD Y CRRE +S Q +CS+++ AAI C+ G Y+V F ++L C P C+ GG F +CSRTC+STC +L + C++ CFPGC+C + Y+ G CV +SECTC + P AG + +C C C NG C+ + VCPKNQV+MD V C C +Y+ C ++ + CGCP V K DGTCV + CPC + +EY P + + C +++C ++ W + ++ CEAVC A GDPHY T D + F+FQG C YTL+ GD+S +P IS N+ CG TC KFV+IIIG + Y L RG D P F Y + +E LT +TQ+VSD +GLR+LWDR TRV ++L+ ++ V GLCGNYDG+ N++ + ++AE + A+ CP E N+ + +D+C NP RK+W+E CGV+K+ NG F C+ A+ D V +Y NC++DACGC+ GGDCEC+C+++A FA C+ +G+ VKWR+ + CP+MC+GGK Y C PCPQTC +G HC C EGC CP + Sbjct: 61 QASNSENQCDYDYLEANETVA-SCGD-LSFTLAGFTISGDSVPVSPACANPRASIGDQRSACIRKYILDLATGKPTPQCKFSKPQKTTRRGCCPGYGGEN---CQQVVCFPKDGGCQNGGRCVKPDKCECQSGFQGAMCDED---LAELQKDTRLRYCFSDASC-KVKNTKFGRKTTPFSDCCSAGGNSFG-YSGVCTACTSS-TGTRSLGTPTGHSEIVPFDTCSSFGPNSISTFDGLTYEFEGRCSYLLAGSINPSR-RWFVKVAMVNCDTFKSCQKTLRFRLDDLHEFVAVGQSLQVYQISGLDGAQMLKVNDSFQGLFDDARDYSGV-RFIRRGDSIIMTSRSLG-LRLRWDSIGSVQLTLDRPVHSNQDLQGLCGNYDGDKKNEFGEFANPSAFGNAWKAQDCPDSANVPVHC-PDTKTTASAEMECRQIDSIFFPCHDRIPPQEFFQRCVQDACLAIRRGEPT------SEVVCRHLSAYAGLCSLGGICIDWRSSNLCPTNCSEPNMYWKECASECPTTCANYDQVKSELCQVTTSPRCECEPDYVHHKGKCVRKTDCPCRYNGKEFPAGGTFNTQCESCQCIQGRWSCYKRASCPKTCLVTGSSFRTFDGAQLDFNGRTCEYVLLEPAPNVTDSRLNVTITQRVRTENVLITDPNQ-LANLPKRVHISYAAYRIELVATSLNYG-SRASVFINDEDVTNSLPFSNQDDHLLVKQITSLTTMVKIGSHITVYFDGFRNIEIKANERVRSNVKGLCGNYDGDTENDLHPRNSDMFGSVTELAESYITGVCTDSPIPLRQAAFADQVSGSDHCQLALLTGDSFKACRESNKVDLNGHYLRSCRREVASGGVQGQQFLFCSSLVQAAIECKNQG--YDVDFYRDSVLSLCAPPCRVGGGTFSLCSRTCRSTCSDLE--STEICEEGCFPGCDCQYDSQYRDHHGTCVRKSECTCRDRFNPDSAIYKAGDVLQRSCTNCTCSNGAFVCEDEACEDKVVCPKNQVFMDLVPSCQRCEDYNNCPRSAMLQKRCGCPGGQVKKSDGTCVPANQCPCKLLKKEYPPMHKLKMACKRFVCKDQTWKQIGKDR-KCEAVCKAQGDPHYFTFDKKKFNFQGKCQYTLVSFDGDASRGLPKFHISAQNVPCGSKQVTCTKFVRIIIGDERYTLVRGQEKDEFPAPFASYGVKLEHLTLYTQVVSDVLGLRILWDRKTRVLIYLRKDYRRQVSGLCGNYDGNAGNDFTFNNLPMASVDEWAEKYRTGAESCPAEDGSRNS---NGWRDICEQNPGRKTWSELTCGVIKNPNGPFKDCLLALDDDTVQTFYENCIFDACGCDSGGDCECVCSAMAGFADHCSDVGVHVKWRSMDNCPLMCDGGKRYMPCMEPCPQTCRNLGDEPAPHCQNAACTEGCGCPRERL 1446 HSP 2 Score: 130.183 bits (326), Expect = 3.378e-26 Identity = 120/428 (28.04%), Postives = 182/428 (42.52%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACG----------FSLATCAKFVQIIIGGQTYLLSRGN-------NDTIPLFPKYDIVIEQLTFFTQ-----LVSDSIGLRVLWDRSTRVYLFLKPSFQGN--VQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWA--EQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGD--CECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-EGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC--TPSQFRCDDGKCIE-SSKICDKV 4502 C ++G T DG + F+G C Y L + S + VA + C F L +FV + Q Y +S + ND+ + F + + S S+GLR+ WD V L L N +QGLCGNYDGD +NE+ ++ + F ++ A + P++ P PD K+ A E C + + +F C + + ++ C+ DAC R G+ E +C L+ +A C+ GI + WR+ NLCP C E ++ CAS CP TC C T C C + G+CV T CPC YNG+ + G + C+ CQC+Q +++C + S C TC T S FR DG ++ + + C+ V Sbjct: 273 TCSSFGPNSISTFDGLTYEFEGRCSYLLAGSINPSRRWF----VKVAMVNCDTFKSCQKTLRFRLDDLHEFVAVGQSLQVYQISGLDGAQMLKVNDSFQGLFDDARDYSGVRFIRRGDSIIMTSRSLGLRLRWDSIGSVQLTLDRPVHSNQDLQGLCGNYDGDKKNEFG----EFANPSAFGNAWKA-------QDCPDSANVPVH-------CPDTKTTASAEMEC---RQIDSIFFPCHDRIPP--QEFFQRCVQDACLAIRRGEPTSEVVCRHLSAYAGLCSLGGICIDWRSSNLCPTNCSEPNMYWKECASECPTTCANYDQVKSELCQVTTSPR-CECEPDYVHHKGKCVRKTDCPCRYNGKEFPAGGTFNTQ-CESCQCIQGRWSCYKRAS-CPKTCLVTGSSFRTFDGAQLDFNGRTCEYV 670 HSP 3 Score: 114.775 bits (286), Expect = 1.407e-21 Identity = 138/587 (23.51%), Postives = 231/587 (39.35%), Query Frame = 3 Query: 2976 VCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGC-NSCTNYDTCTD---TSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQG-SCMYTLMDTPGDSSNNIPPITIS---------------VANIACGFSLATCAKFVQIIIGGQTYLLSRGN--------NDTIPLFPKYD-IVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNE-YMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYY-SNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKW---RTQNLCPIMCE-GGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCP--NGMIDLNGECVPPTTCPC---FYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGT 4616 +CI+ R + NC+ P N + + S C +C NYD + + C C D V G CV+ +DCPC Y +E+ +C C W+ + A+C C+ G ++T DG F G +C Y L++ + +++ +TI+ +AN+ ++ A ++++ Y SR + +++P + D ++++Q+T T +V + V +D + + + NV+GLCGNYDGD +N+ + + + + AES+I +C P A + D+ S ++ C + T F +C E+ D+N +Y +C + G C+SL A +C G V + +LC C GG + C+ C TC + S + C GC C + D +G CV + C C F + + +C C C F C ++ + C +Q D V C ED NC + Q +C G KK DGT Sbjct: 534 ICIDWRSSNLCPTNCSEP----NMYWKECASECPTTCANYDQVKSELCQVTTSPRCECEPDYVHH-KGKCVRKTDCPCRYNGKEFPAGGTFNTQCESCQCIQGRWSCY--KRASCPKTCLVTGS-SFRTFDGAQLDFNGRTCEYVLLEPAPNVTDSRLNVTITQRVRTENVLITDPNQLANLPKRVHISYAAYRIELVATSLNYG-SRASVFINDEDVTNSLPFSNQDDHLLVKQITSLTTMVKIGSHITVYFDGFRNIEIKANERVRSNVKGLCGNYDGDTENDLHPRNSDMFGSVTELAESYITG--VCTDSPIPLRQAAFA----------DQVSGSDH-CQLALLTGDSFKACRESNKVDLNGHYLRSCRREVASGGVQGQQFLFCSSLVQAAIECKNQGYDVDFYRDSVLSLCAPPCRVGGGTFSLCSRTCRSTCSDLES---TEICEEGCFPGCDCQYDSQYRDHHGTCVRKSECTCRDRFNPDSAIYKAGDVLQRSCTNCTCSNGAFVCEDEACEDKVVCPKNQV------------FMDLVPSCQRCEDYNNCPRSAMLQKRCGCPGG---QVKKSDGT 1080
BLAST of SCO-spondin vs. Ensembl Cavefish
Match: scospondin (subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]) HSP 1 Score: 526.168 bits (1354), Expect = 9.635e-154 Identity = 421/1479 (28.47%), Postives = 670/1479 (45.30%), Query Frame = 3 Query: 510 CQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTG-SWGSQSG-SCVSC----------------KANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-----GFGCPDIANTLSTCKPDDQIL----ANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKI-VTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAG-------DNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDD-----CFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDT----CTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFP--------KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPE-NNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDG--KCIESSKICDKVTDCSNSEDE----KNCN-VCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDC 4736 C E +AG C + + + C+ + C F +S ++CC G SW + S +C+SC K + G + N + TC S+G +YR+FD + + +QG C+Y+L + +D TW++ I C C K + + G +V SN L L + + + G ++ L +F+ + G +++K+D +N ++++++ + GLCG D+ S ASFGNS++ GC D A +C + A C +++ F C V+ Y C+ C+ + +C L++Y+ C+ + + WR LCE CPG +++SDC S CP +C + S C+ VG C C G + C++ + CPC + + G + CNTC C W C+ + C + C I+G TFD + GCQ++ VE V+ + V I++ G G ++ + TV VN S+ +V ++G+ LP D + VR+ T+ + V+V L+ + I + + + GLCG N +DFT + + S+ + A + GSC + DP + +C+ +L+ F C + V++E Y C+ E S +L +C+ + A C + G+ S P+ +GG V+Q C R C +C +L ++ C+++ C PGC+CP + GQCV C C + P G+++ INC TCVC G NC CP + ++ S +C++ D+ CT+ C CP TV D CV P +CPC + R + ND I+ C +C R W+ + C C+A GDPHY T DG+ +SF G C Y L ++S N+ CG + TC K V + +G L RG + T+ P +V+E++ F L S +G+ +LWD RVY+ L+P QG V GLCGN+DGD +N++ + ++ N+ +++SP +VA +D C NP R +WA++ C V+ T +F+ C H +V +YY C++DACGC+ GGDCECLCT++A +A +C G+ + WR+Q LCP+ CE G Y+ C S C C + + SHCS CVEGC CP+G + C+ P+ CPC + G + GA++ ++C+ C C ++CT + C S+F+C G +CI S +CD DC + DE C VC ++F+C S+ CI +CDG +C D SDE C C + C + CI K++CDG+ DC Sbjct: 104 CCEGWAGPHCSQGVSV--------RGLCFSTWNCEGFPGVQNF-SLMSIEQCCGSLWGLSWKNASDQTCLSCSYTLLPDPQTSPLLLRKGHLGGLH--NPKASATCMSWGGVHYRSFDRKHFHFQGGCTYVLASSTDG---TWAVYI-STVCDGGGHCSKALQMMLGRDLVS--------VNKSNMSLNGLIIAQGE-PVFQNGVSIHWL--GDFVFVESGLG-VRIKFDLSNTVYLTVNTEHFAA-TRGLCGVYNNNAEDDFTGRSGSISQYAASFGNSWRVPDQQTEGCSDAAELGHSCDVTGDVSLRRGAESACHQLKESPFSHCHHQVDPGPYVDTCLYVYCSLGEKERV--------GAVCDTLASYARECAQQHIILSWRRPDLCERVCPGGQLFSDCVSSCPPSCSSALPPASGQCREECVGGCECPPGLYLHRGQCLRKDDCPCFHRRHTYQEGDTIKQKCNTCVCQGGQWQCSSERCAAQCSIIGAMHISTFDKKSYNLQAGGCQFIVVEDFVDK-----KLAVTIRAGECVTGGRTGCPQEMSVTALHTTVTVN---------------SKGSVILNGQPEA--LPVLTAD--LAVRRATSSFLLVQVFGARLLWHTDGSLVVITLEPGFAHKVRGLCGTLTWNQHDDFTTPEGDVENSVPSFAAKFNIGSCPLSSAVTSDPCSTYTQRRQYAESVCA-VLHSAVFQACHDV--VEREPYVRMCQTEVCSCATHRLCHCTVVTAYAQHCAQEGV-----LVSWRNHTFCPVQCSGGQVYQECGRACGGSCADLQQSWV--CEENAGSRTCVPGCQCPEGLMQDDQGQCVPVDMCPCVHGGAVHQP----GSSVQINCNTCVCGKGVWNCSDDPCPEVRECPGSLIFSPG-SCLRTCSSLDSPQALCTEP---IYGCVCPEGTVLLGD-QCVTPDECPCHHNGRLFFTNDTISKDCNTCVCKQRRWHCTQQL---CAGTCVATGDPHYVTFDGRCYSFLGDCEYMLAQEMSGL------FSVSAENVPCGSTGVTCTKSVTLTVGNTAIHLLRGKSVTVNGMPVTLPKSYSGSGLVLEKVGMFVSL-SSRLGVTLLWDGGMRVYVRLEPHLQGRVGGLCGNFDGDTENDFT----TRQGIVESTPELFGNS----WKVSPSCPDVAEQDLRDPCVINPHRVTWAKKKCAVI--TQELFSLC----HAEVPYQQYYDWCVFDACGCDSGGDCECLCTAIAAYAEECNRRGVYIHWRSQELCPLQCEDGLVYEPCGSTCSAVCPSSTPSVHSHCSPLSCVEGCFCPHGTVRHGDGCITPSECPCEWEGSLFPAGAVL-TQHCQNCTCSNGTWDCTGPACPPLVPCLESEFQCTRGSQRCIPSIWLCDNEDDCGDGSDEICPSTYCGVVCAEEEFRC-SSSRCILYLHRCDGHDDCGDFSDERGCVCSLGEFQCPGDQ-CIPAKRVCDGHRDC 1476 HSP 2 Score: 60.8474 bits (146), Expect = 1.516e-8 Identity = 70/257 (27.24%), Postives = 99/257 (38.52%), Query Frame = 3 Query: 2724 CQAGGTVFQI-CSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC------------------------------------------KTKDN--CTNPVCPKNQVYMDSVSGCN-SCTNYDTCTD--TSSIANSCGCPNDTVSKIDGTCVQPSDCPC-----------IYMNR---EYAPNDVITIKCAKYICSNRMWNLLSEETANC 3308 C+ G V++ C+ C +C +L E C PGC CP + G+CV + C C P+ E+ G N+T++C TCVCING L C +T+ N CT P CP QV+ C SC + ++ + + C CP TV +GTCV S+CPC I +N E P V+ C +C N ++N E C Sbjct: 4496 CEDRGRVYEANCANQCPRSCADLWEHVQCL-QGTCHPGCRCPDGWLLQ-EGECVRVTACRCGLPTENGTLELSPGQNVTVDCNTCVCINGSLVCTNQPCPTYGSWGSWSECSVSCGTGQRIRRRSCTHKPGGPPCSETEQNQTCTQP-CPAGQVFSACAGSCPFSCQDLWPESECVPGACSPGCACPPATV-MFNGTCVPHSECPCSPVSLLLTSPNITLNALPDEVPPGTVVPHLCNFCVCDNGVFNCTEESCDAC 4748 HSP 3 Score: 60.077 bits (144), Expect = 2.798e-8 Identity = 61/185 (32.97%), Postives = 79/185 (42.70%), Query Frame = 3 Query: 2706 QACIPLCQAGGTVFQICSRTCQSTCRELNEATASKC-DDDCFPGCECPPNYYKTGTGQCVSESECTCWNPS----------RPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSV--------SGCNSCTNYD--TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPC 3197 Q C C AG VF C+ +C +C++L S+C C PGC CPP G CV SEC C S E+P GT + C CVC NG NC T+++C CP+ + + SV GC + CT T I+ C C + DG CV P+ C C Sbjct: 4635 QTCTQPCPAG-QVFSACAGSCPFSCQDL--WPESECVPGACSPGCACPPATVMF-NGTCVPHSECPCSPVSLLLTSPNITLNALPDEVPPGTVVPHLCNFCVCDNGVFNC-TEESCD--ACPEGERWRRSVLEMDRVCERGCVDMYRAEPLNCTSTHGISEGCVCEDGRYRSTDGRCVIPALCQC 4812 HSP 4 Score: 51.9878 bits (123), Expect = 8.371e-6 Identity = 32/93 (34.41%), Postives = 45/93 (48.39%), Query Frame = 3 Query: 4173 PCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQF 4451 PCP TC + S +CS HC+ GC CP G+ +G CV + C C + G G + ++C C C +C D S CI+ C S + Sbjct: 2506 PCPLTCSDLHSE--RNCSH-HCLTGCRCPAGLFLQDGHCVNASKCLCLWEGSELQPGEEVTPDSCTTCMCQDGHVSC--DNSSCISKCDWSAW 2593
BLAST of SCO-spondin vs. Ensembl Cavefish
Match: vwf (von Willebrand factor [Source:NCBI gene;Acc:103041546]) HSP 1 Score: 328.176 bits (840), Expect = 3.594e-90 Identity = 330/1301 (25.37%), Postives = 539/1301 (41.43%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVV-VKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG----CPDIANTLSTCKPDDQILAN--QQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQ-----SNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVS--SHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGD-------NDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKD-QLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC---------------------KTKDNCTNPVCPKNQVYMDSVSG-----CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIP-PITISVANIACGFSLATCAKFVQII-IGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPE--NNV-APSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK---------HYDACASPCPQTCDTIGSNAPSHCS-TTHCVEGC--SCPNGMI--DLNGECVPPTTCP-CFYNGQSYVNGAIIKMEN-----CKKCQCVQAKFNCT 4406 E C +G + TFD +Y + G CSY L D T+SI + G K + + G+ + S G+L PL P + ++ + + ++ + F + A NI+L ++ GLCGN ++ P+++++ + + F NS+ G C + TC + +C +R F CA LV++D + ++C ++C + +C L Y+ +C+ G + W + + C +CP +S+C+ C +C++L C+ V C C G+V G C++ C C + G+ +PPG+ DCNTC C W CT + CP C + G + + TFD F F+ CQY+ + D + N+F I ++ + + T + +E TV++ + V +DG D +P N +R + + + V+++ +L +DG + +++S + GLCG ++GN +DF + + ++ + CDN + DP + + + C+ +L+ P+F C + +V+ Y CR + S D Q C+A+ N A AC G+ + Q L G V+++C C TCR S C C GC CP Y T TG+CV +C+C P + +I C C NG + C + + + + P P Q+ +SV +C N D + + C CP TV + CV P CPC + N Y+P I I C +C R W C+ VC GD HY + DG F+F G C Y L D N + I V N ACG C K + + +GG + P+ + D+ I + F+ +V + + WD TR+ L + ++G V GLCGN+DG N+ + + E D + F S +++ P N V PS+ C+ + ++ EQ C V+ + +F C + D Y+ C +D C C GDC C C ++A +A +CA G+ V WR+ +LCP+ CE ++AC + CP+TC P S C+EGC SCP G I ++ CV P+ C C +NG+ +G I + + C+ C C + C Sbjct: 23 EGGGRCSLFGRHHVHTFDGVIYEFPGDCSYKLAG--DCHHRTFSILGDLSH-----GRRKGVTVFLGETFELHLSVGGVLSQGAERLPL-PYASSSVFAGFELGSVRLWS-EEFGFSIRIDPAHNIQLTLSKHHA-----------NHTCGLCGNYNSVPTDEYTAQEGFLTEDPYDFANSWAMNGASEACKRVTPPAQTCNNTGASARDLMSRCETLRTSPVFLHCAHLVDTDAFAAVCESDVCHCVDGQ----------ECVCQALLEYARTCASHGLVLPNWHTQSQCTPKCPIGMQYSECSQACSTSCQSL--NIHEVCREECVDGCSCAVGKVLDGDRCVEVSQCSCQHTGKRYPPGSTISQDCNTCVCRHGSWECTNEECPGQCVVSGQSHYKTFDDKFFTFSGF-CQYLLAK------DCQTNLFSAIIDTVQCADDQDAVCTRSVTLKFHELANQTVRLKHGGV---------------VSVDGLD------VQMPLINGLLRVQSTVLSSVRLNYGDDLQLDWDGRGRVVLRLSPVYAGKMCGLCGNYNGNQGDDFLTAGGLVETRVEGFGNAWKMNAECDNIQKQPSDPCSLNPKRVRFAEEACALLLS-PKFEPCHH--EVNPYPYLKNCRYDVCSCADGQECLCAAVSNYAAACSARGV-----LINWRGQGYCELTCPGNQVYEVCGSVCNQTCRS-RSLPDSACRSMCEEGCFCPRGLYLTDTGECVPPDQCSCHYDGEIYQPNDMFADHHSI----CYCENGSMRCSSSELPASVLSDLFFDEPPLSRERRSVSCPA-PLQQLRCESVRDVGIECARTCQNLDLECVSQGCISGCVCPPGTV-RHRRECVPPDQCPCFHNNHAYSPGQSIKIDCNTCVCGGRQWKCTQRV---CDGVCHTVGDSHYISFDGLKFTFPGPCQYILAQ---DYCNGLDGTFRILVENSACGTIGHRCRKSISVFYMGGLIVMEQEEVKMKKPVMKETDVEIVRSGFY-YIVLLGKNISITWDTGTRIILQISGQYRGKVCGLCGNFDGSQNNDLLSSNNQIEVDPLDFGNS---------WKVQPSCANAVQVPSQ----CSTDMSKQVTVEQSCRVLN--SDLFRECNSVV--DPEPYWEICTHDTCSCASVGDCACFCDAIAAYAHECAQKGVVVDWRSNDLCPLSCEELNKDAGVECMWRFNACDTACPKTC-----QHPEQLSCPIACLEGCHASCPQGQILDEVLRRCVEPSECQVCMHNGERISHGKQIILNHGDPHLCQICHCEENTLTCA 1219 HSP 2 Score: 101.293 bits (251), Expect = 7.626e-21 Identity = 101/375 (26.93%), Postives = 154/375 (41.07%), Query Frame = 3 Query: 3318 CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKF-VQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFT-QLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIAN--ADICPYEISPE---NNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 C +G H T DG ++ F G C Y L GD + I +++ G ++ F + + +GG +LS+G + +PL V + +L S+ G + D + + L L + GLCGNY+ +EY ED FA S+ N ++ C P NN S M R T+ VF C + D + + + C D C C G EC+C +L +A CA G+ + W TQ+ C C G Y C+ C +C ++ N C CV+GCSC G + CV + C C + G+ Y G+ I ++C C C + CT + Sbjct: 28 CSLFGRHHVHTFDGVIYEFPGDCSYKLA---GDCHHRTFSILGDLSHGRRKGVTVFLGETFELHLSVGG---VLSQGA-ERLPLPYASSSVFAGFELGSVRLWSEEFGFSIRIDPAHNIQLTLSKHHANHTCGLCGNYNSVPTDEYTAQEGFLTEDPYDFANSWAMNGASEACKRVTPPAQTCNNTGASARDLMSRCETLR-------------TSPVFLHCAHLV--DTDAFAAVCESDVCHCVDGQ--ECVCQALLEYARTCASHGLVLPNWHTQSQCTPKCPIGMQYSECSQACSTSCQSL--NIHEVCR-EECVDGCSCAVGKVLDGDRCVEVSQCSCQHTGKRYPPGSTIS-QDCNTCVCRHGSWECTNE 374 HSP 3 Score: 92.4337 bits (228), Expect = 4.643e-18 Identity = 100/410 (24.39%), Postives = 167/410 (40.73%), Query Frame = 3 Query: 699 SGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVI---EAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLE---AGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLS---TCKPDDQILANQQCS-KIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSG-EVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPST 1877 S C +C N E C G +Y+TFD++ +T+ G C Y+L + + I+ V D C + + + E + V+ G++ + D + PL I G +LR + +L S+ +++L WD + + +Y G + GLCG D+ + V FGN++K C +I S + P A + C+ + KF C VN Y C ++C+ + + LC +S Y+ +CS +G I WR CE CPGN+++ C S C TCR+ P + C++ C C G + C+ P+ C C YDG+ + P + + C C + C+ P++ Sbjct: 356 SQDCNTCVCRHGSWECTNEECPGQCVVSGQSHYKTFDDKFFTFSGFCQYLLAKDCQTNLFSAIIDTVQCADDQDAVCTRSVTLKFHELANQTVRLKHGGVV-SVDGLDVQMPL----------ING----LLRVQSTVLSSVRLNYGDDLQLDWD-GRGRVVLRLSPVYAGKMCGLCGNYNGNQGDDFLTAGGLVETRVEGFGNAWKMNAECDNIQKQPSDPCSLNPKRVRFAEEACALLLSPKFEPCHHEVNPYPYLKNCRYDVCSCADG----------QECLCAAVSNYAAACSARGVLINWRGQGYCELTCPGNQVYEVCGSVCNQTCRSR-SLPDSACRSMCEEGCFCPRGLYLTDTGECVPPDQCSCHYDGEIYQPNDMFADHHSICYCENGSMRCSSSELPAS 738
BLAST of SCO-spondin vs. Ensembl Cavefish
Match: otog (otogelin [Source:ZFIN;Acc:ZDB-GENE-120228-1]) HSP 1 Score: 325.479 bits (833), Expect = 2.235e-89 Identity = 312/1192 (26.17%), Postives = 517/1192 (43.37%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYEN---CTSD----TGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGT--PMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXV-----ASFGNSF-----KGFGCPDIANTL-STCKPDDQ--ILANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDT-LCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKV---GRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQ-FDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSK-SIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS------------YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPP-NYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD--------SVS---GCN-SCTNYD---TCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP--LFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHG-TMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCE 4142 E TC S+G Y TFD Y + G+CSY L + + S+ I N CTS T V + + G++ ++ L+VT ++ +P + + + +++L+S + L W N + + G GLCGN + + ++D V A FGNS+ + C I S C D +L ++ LK F C + V+ +Y + C ++C N DV+C + Y+ +C+ + +WR+ +C +CP + +C CPV CR C +SK+ C C + C+K CPC + G +P G Q +CN CTC+ W+CTE+ CP C + G F +FDG + F CQYV + + + V IQ+ + G + A I +V + +++ L + SQ + LP++ D +++L++++ +VK S L ++ + +Y+++ + +++ +GLCG F+GN ++DF I + ++ ++ +C + P D C T ++ + +V + C S QL CSA+ + A CR+ + E F S++ + C C A + +C +CQ C L+ + C D+C GC CP YY T T CV +EC C P G I + +C +G++ ++ T+ +CP Q+Y D SVS C+ +C +Y TC+ C CP + D C +P CPC++ +EY P D ++ C +C + E C ++C AYGD HY+T DG +F + G+C L+ + D ++++ NI C S C K + I +G + P + + +++I +FT + L VLWDR T +++ P +QG + GLCGN+D NE M +F S+ A + +I + C+ NP R+++A++ CG++ + VF C + DV +Y NCL D CGC+RGGDCEC C+S+A +A +C G+ V WR+ +LCP CE Sbjct: 127 EREMTCRSWGQYNYETFDGLYYYFPGKCSYTLLKDCE-ENIQPSLLIQVHNDPECTSFPYSCTRSVSLFLPWEGEIRLQD-----------------LNVTFKGQSVKLPHNIHDLELEQIADYVLVS-QPQVFTLAWHGLNGSIYIKMSPEFVGRTCGLCGNFNADVNDDLKTSYGVFTEDLAMFGNSWVEEEPQKATCSMIPYLYPSPCSGQDSHVLLKVEEVCATLLKEPFKSCHEFVSPYSYMASCSNDLCLSGPNG----------DVVCQVFTEYARACAHADHPLNKWRTHVPVCGVECPAELQYRECIPCCPVHCRT-----EQMCIDSKLQCLDGCYCQDDLIYEDGLCVKASDCPCEHRGMLYPSGHTIQEECNNCTCSGGAWNCTEQKCPGECSVTGDMYFQSFDGRVYTFL-ATCQYV-----LAKSRNSGGFTVTIQN---APCGPNLDGACIQSVNVVLNEDPRTEVTLSHSGEVFMASQFRI---------SLPYS--DDVFQIQELSSLFVQVKTSQGLLLQYNWREFRLYLQLGELWRDDTVGLCGTFNGNIQDDFLAPSGMIESTPQLFGNAWRVSSAC------VPRPSLPQLDPCDTHQQAASYA--VEKCEVLMQEVFAVCHEHVSPVGFQLQCRADVCKCGTPCLCSALAHYARTCRKHNVIIE--FRSHVPE-CSVACPATME-YGLCVSSCQRRC--LSFSMPQSCGDECEEGCVCPQGTYYSTHTHSCVKRNECPCSFLGTDYSP----GDVIMTSSGVQICRDGQIVLQS--TITDKLCPPGQIYEDCGNMVDGPSVSKGLACDRTCESYLLNLTCSTHEPCVPGCVCPTGLLKHGD-ECFEPVACPCLWKGKEYYPGDKVSSTCHLCVCQQGTFQC---EFRPCPSMCTAYGDRHYRTFDGLLFDYVGACKVYLLKSSADVM-----VSVTTENIDCFESGVICRKALLISVGRSFIVFDDETGKPSPSSVIDRQNVLIWSAGYFTIVHLSQYELSVLWDRKTTIHIQAGPRWQGKLSGLCGNFDLKTVNEMRTPDNMDSATPQEFGNSWTAAECVNSPDI-----------RHPCSLNPLREAFAKKQCGIL--LSEVFQVCHPVV--DVTWFYMNCLADTCGCSRGGDCECFCSSVAAYAHRCCHQGVTVDWRSPSLCPYDCE 1220 HSP 2 Score: 130.568 bits (327), Expect = 9.936e-30 Identity = 135/516 (26.16%), Postives = 215/516 (41.67%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCN-SCTNYDTCTDTS-SIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSL-ATCAKFVQIII------------GGQTYLLSRGNNDTIPLFPKYDIV--IEQLTFFTQLVSDSIGLRVLWD-RSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQ-DMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQ-NLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCD-----DGKCIESSKICDK 4499 CP Y + + C C C D+ + C C +D + + DG CV+ SDCPC + Y I +C CS WN ++ C C GD ++++ DG++++F +C Y L S N T+++ N CG +L C + V +++ G+ ++ S+ P D V I++L+ V S GL + ++ R R+YL L ++ + GLCG ++G++Q++++ + E Q NA + +S PS Q D C+ + S+A + C V+ VFA C E H + C D C C CLC++LA +A C + +++R+ C + C Y C S C + C + + P C C EGC CP G CV CPC + G Y G +I M + C + +D + C P Q D DG + CD+ Sbjct: 403 CPAELQYRECIPCCPVHCRTEQMCIDSKLQCLDGCYCQDDLIYE-DGLCVKASDCPCEHRGMLYPSGHTIQEECNNCTCSGGAWNCTEQK---CPGECSVTGDMYFQSFDGRVYTFLATCQYVLAK-----SRNSGGFTVTIQNAPCGPNLDGACIQSVNVVLNEDPRTEVTLSHSGEVFMASQFRIS----LPYSDDVFQIQELSSLFVQVKTSQGLLLQYNWREFRLYLQLGELWRDDTVGLCGTFNGNIQDDFLAPSGMIESTPQL----FGNA----WRVSSACVPRPSLPQLDPCDTHQQAASYAVEKCEVLM--QEVFAVCHE--HVSPVGFQLQCRADVCKCGT----PCLCSALAHYARTCRKHNVIIEFRSHVPECSVACPATMEYGLCVSSCQRRC--LSFSMPQSCGD-ECEEGCVCPQGTYYSTHTHSCVKRNECPCSFLGTDYSPGDVI-MTSSGVQICRDGQIVLQSTITDKL--CPPGQIYEDCGNMVDGPSVSKGLACDR 883 HSP 3 Score: 103.219 bits (256), Expect = 2.004e-21 Identity = 109/387 (28.17%), Postives = 167/387 (43.15%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNI-PPITISVAN-IACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP----LFPK--YDIVIEQLTFFTQLVSDSIGLRVLWD-RSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-EDAIQFAESFI------ANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQ-NLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHC--STTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 E C ++G +Y+T DG + F G C YTL+ D NI P + I V N C +C + V + + + + + N T P +D+ +EQ+ + LVS + W + +Y+ + P F G GLCGN++ D+ ++ + ED F S++ A + PY + P +P QD ++ K E++C + F SC E + Y ++C D C GD +C +A CA P+ KWRT +C + C Y C CP C T C S C++GC C + +I +G CV + CPC + G Y +G I+ E C C C +NCTE Sbjct: 129 EMTCRSWGQYNYETFDGLYYYFPGKCSYTLLK---DCEENIQPSLLIQVHNDPECTSFPYSCTRSVSLFLPWEGEIRLQDLNVTFKGQSVKLPHNIHDLELEQIADYV-LVSQPQVFTLAWHGLNGSIYIKMSPEFVGRTCGLCGNFNADVNDDLKTSYGVFTEDLAMFGNSWVEEEPQKATCSMIPY-LYP----SPCSGQD---SHVLLK--VEEVCATL--LKEPFKSCHEFV--SPYSYMASCSNDLCLSGPNGDV--VCQVFTEYARACAHADHPLNKWRTHVPVCGVECPAELQYRECIPCCPVHCRT-----EQMCIDSKLQCLDGCYCQDDLIYEDGLCVKASDCPCEHRGMLYPSGHTIQ-EECNNCTCSGGAWNCTE 489 HSP 4 Score: 55.4546 bits (132), Expect = 7.736e-7 Identity = 44/146 (30.14%), Postives = 62/146 (42.47%), Query Frame = 3 Query: 4134 MCEGGKHYDACAS-----------PCPQTCDTIGSNAPSHCSTTH-CVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTP---SQFRCDDGKCIESSKICDKVTDCSNSED 4526 +C G+ Y+ C + C +TC++ N CST CV GC CP G++ EC P CPC + G+ Y G + C C C Q F C ++ C + CT +R DG + C KV +S D Sbjct: 856 LCPPGQIYEDCGNMVDGPSVSKGLACDRTCESYLLNL--TCSTHEPCVPGCVCPTGLLKHGDECFEPVACPCLWKGKEYYPGDKVS-STCHLCVCQQGTFQC--EFRPCPSMCTAYGDRHYRTFDGLLFDYVGAC-KVYLLKSSAD 995
BLAST of SCO-spondin vs. Ensembl Cavefish
Match: ENSAMXT00000041370.1 (pep primary_assembly:Astyanax_mexicanus-2.0:5:1809499:1822580:1 gene:ENSAMXG00000037407.1 transcript:ENSAMXT00000041370.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 318.546 bits (815), Expect = 1.785e-87 Identity = 328/1262 (25.99%), Postives = 532/1262 (42.16%), Query Frame = 3 Query: 750 NNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVV-VKSS--GNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG----XXXXXXXXXXXXXXXVASFGNSFKGFG----CPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGS-CIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYT---CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKL-----PFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSK--SIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYIT-GSCDNTPQSIVDP--PAGDND-----ICSTILNGPE-FSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDD--CFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC---KTKDNCTNPVCPKN---QVYMDSVSGC-NSCTNYD-TCTD-----TSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSI-GLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC-------EGGKHYDACASPCPQTCDTIGSNAPSHCST-THCVEGC--SCPNGMIDLNGE---CVPPTTCPCFYNGQSYVNGAII 4352 + N C ++G +++T+D ++ C++ILT+ S ++I + + + K+ + G VV VK+ G+G+ T S+ + +V T + +D+ +++ L A I GLCG N + +GN +K G C + ++ D+ N SK F C DLV+ + + +C++++C + ++ S LC L+ YS C G WR++ C +CP +K++ +C S C TC N + C + C C S V T CI +C C++ G+ + G C CTC+ W+C +K CP TC + G TFDG + F+ C YV + TD F I+ +V + S+ LKA + L + TNV + E + L T + +I + K ++ Y ++ + +L + +YI + + GLCG F+ +DFT + V+ A ++ T SC + ++ +P + +N+ CS + + FS C S+++ Y + K + C+A+ + AC GI + ++ L V+ +C +CR L++ S C GC C Y +CV S C C++ + P G ++ TC C G+LNC CT+P+ N V + S C SC D C T + C CP+ VS +G C+ CPC++ Y P + + C C +R W + + C A C YGD HY T D + + F G+C YTL SSN + NI CG + TC+K +++ IG +LS G+ + +I + T LV ++ G+ ++WDR T +Y+ L P F G V GLCGN+DG+ N++ T + ++ + A F + P PE V Q+ C NP R++WA++ C ++ + VF++C + D +YS C+ DAC C+ GGDCEC CT++A +A C +G+ V WRT +CP+ C E HY C +PC +TC N CS +EGC CP G+ + + CV C C+ + Y NG + Sbjct: 22 SEHNGQVCSTWGNYHFKTYDGDVFQLPSTCNHILTSQCRGSYEAFNIQLRRQVENGEPTISKITMKLDGTVVDVKAPHLGSGVFIETISSYIMVKATVGLTA-----------MWNEDDALMIELNAKFIN--------------------QTCGLCGDFNGVPTYNEFIKDGAQLSATDYGNLWKMDGPTETCEEQPERVNENCGDENFCQNLFLSK---AFSSCTDLVSVEPFTKVCIEDLCQCNSSAG--------SSCLCDTLAEYSRQCVHAGGQPANWRTEHFCPMKCPESKVYMECGSPCMDTCSNPER--GQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCIHGGKAYASGEGYSSSCRECTCSGGQWNCVDKQCPGTCSVEGGAHIITFDGKPYTFHG-DCSYV-LSKECTGTD-----FTILG-------------------DLVKCGITDSETCLKAVTLALSNG-TNVSLSCELNINLLFKKIFRLTQTESDISIFKPSSFYIVIQTTIGLRLEIQLTPIMQVYITADPKFQGQMCGLCGNFNNIQADDFTTMGGLTEGTAVDFANTWKTRASCLDVQRTFENPCSLSTENEKYAQYWCSLLSDASGVFSSCH--SEINPATYKNCIYDTCNCEKTEDCMCAALSSYVHACAAKGIY--IKGWRDVACKSYTLSCPNTMVYSYNMTSCGRSCRSLSQTDFS-CSSQFVAVDGCGCAEGTYLNEKEECVDPSGCPCYDSNTVIQP----GETVSREGTTCTCKQGQLNCIGSGKVQECTSPMTYLNCSAAVPGSTGSECAKSCKTLDMACVSYHIKYTRGCVSGCVCPSGLVSDDNGGCITEDLCPCVHNGASYKPGETVKEDCNTCTCKDRRWQCTTNQ---CSATCSIYGDGHYITFDDKRYVFNGNCEYTLAQDFCSSSNTNGTFRVITENIPCGTTGTTCSKAIKLFIGSNELVLSDGSYQVVQRDAGQEIPYQIRTMGNYLVIETNNGVVLIWDRKTSMYIKLTPDFNGRVCGLCGNFDGNANNDF---TTRSQELVVNALDFGNSWKFSP--TCPEAMVV----QEPCAHNPYREAWAQRQCSIIN--SDVFSACHSKV--DPASFYSACVRDACACDTGGDCECFCTAVAAYAEACNAVGVCVAWRTPKVCPLFCDYYNPPGECEWHYKPCGAPCMKTC----RNPAGTCSLQLPAMEGCYPECPAGLPFFDEDTMKCVALEECGCYVGMEHYANGQTV 1183 HSP 2 Score: 150.984 bits (380), Expect = 6.429e-36 Identity = 138/492 (28.05%), Postives = 220/492 (44.72%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCNSCTNYDTCTDTSS-------IANSCGCPNDTV--SKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMD--TPGDSSNNIPPITISVANIACGFSLA-TCAKFVQIIIGGQTYLLSRGNNDTIP-------LFPKY--------DIVIEQLTFFTQLVSDSIGLRVLWDRS--TRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVN-RYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQ--NLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGM-IDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 CP+++VYM+ S C DTC++ + C CP+DTV + C+ S+C CI+ + YA + + C + CS WN + ++ C C G H T DG+ ++F G C Y L T D TI + CG + + TC K V T LS G N ++ LF K DI I + + F ++ +IGLR+ + +VY+ P FQG + GLCGN++ +++ G + A+ FA ++ A + + EN C+ + + + +A+ C ++ D +GVF+SC H ++N Y NC+YD C C + DC +C +L+++ CA GI +K WR + C Y + C ++C ++ S S V+GC C G ++ ECV P+ CPC+ + G + E C C Q + NC Sbjct: 273 CPESKVYMECGSPC-----MDTCSNPERGQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCIHGGKAYASGEGYSSSCRECTCSGGQWNCVDKQ---CPGTCSVEGGAHIITFDGKPYTFHGDCSYVLSKECTGTD-------FTILGDLVKCGITDSETCLKAV-------TLALSNGTNVSLSCELNINLLFKKIFRLTQTESDISIFKPSSFYIVIQTTIGLRLEIQLTPIMQVYITADPKFQGQMCGLCGNFNNIQADDFTTMGGLTEGTAVDFANTWKTRASCLDVQRTFENP---------CSLSTENEKYAQYWCSLLSDASGVFSSC----HSEINPATYKNCIYDTCNCEKTEDC--MCAALSSYVHACAAKGIYIKGWRDVACKSYTLSCPNTMVYSYNMTSCGRSCRSLSQTDFSCSSQFVAVDGCGCAEGTYLNEKEECVDPSGCPCYDSNTVIQPGETVSREG-TTCTCKQGQLNC 726 HSP 3 Score: 109.383 bits (272), Expect = 2.831e-23 Identity = 95/378 (25.13%), Postives = 157/378 (41.53%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDG-DLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTN--------GVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFL-GIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI---DLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 VC +G+ H+KT DG +F +C + L S A L + + I T L D + IE ++ + +V ++GL +W+ + + L F GLCG+++G NE++ +D Q + + N +++ P+ + C P+R + CG D N F+SC + + V + C+ D C CN CLC +LA ++ QC G P WRT++ CP+ C K Y C SPC TC + HC++GC CP+ + N C+P + C C + G++Y +G +C++C C ++NC + Sbjct: 28 VCSTWGNYHFKTYDGDVFQLPSTCNHILTSQCRGSYE------------AFNIQLRRQVENGEPTISKITMKLDGTVVDVKAPHLGSGVFIETISSYI-MVKATVGLTAMWNEDDALMIELNAKFINQTCGLCGDFNGVPTYNEFI------KDGAQLSATDYGNL----WKMD-----GPT---ETCEEQPER---VNENCG---DENFCQNLFLSKAFSSCTDLV--SVEPFTKVCIEDLCQCNSSAGSSCLCDTLAEYSRQCVHAGGQPANWRTEHFCPMKCPESKVYMECGSPCMDTCS---NPERGQVCGEHCIDGCFCPSDTVFDDVTNTGCIPLSNCSCIHGGKAYASGEGYS-SSCRECTCSGGQWNCVD 362 HSP 4 Score: 65.4698 bits (158), Expect = 6.144e-10 Identity = 95/408 (23.28%), Postives = 159/408 (38.97%), Query Frame = 3 Query: 699 SGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSN-----NPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFG-CPDIANTLS---TCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCET---QCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEY---GYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHC 1853 S SC C + N V+ + TC G + TFD + YT+ G CSY+L+ + T ++V T C+K + + S+G + + + N + L+ T +D ++ P + V++ + L ++ I + + + G + GLCG AD+ + F N++K C D+ T + +++ A CS + F C +N TY C+ + C + D +C LS+Y +C+ KG I+ D C++ CP ++S + C +CR+L QT + C + V CG E Y C+ P CPC PG + TCTC +C Sbjct: 345 SSSCRECTCSGGQWNCVDKQCPGTCSVEGGAHIITFDGKPYTFHGDCSYVLSKECTGTDFTILGDLVKCGITDSETCLKAVTL------ALSNGTNVSLSCELNINLLFKKIFRLTQTESDISIFKPSSFYIVIQTTIGLRLEIQLTPIMQVY--------ITADPKFQGQMCGLCGNFNNIQADDFTTMGGLTEGTAVDFANTWKTRASCLDVQRTFENPCSLSTENEKYAQYWCSLLSDASGVFSSCHSEINPATY-KNCIYDTC----------NCEKTEDCMCAALSSYVHACAAKGIYIKGWRDVACKSYTLSCPNTMVYSYNMTSCGRSCRSLSQTDFS-CSSQFVAVDGCGCAEGTYLNEKEECVDPSGCPCYDSNTVIQPGETVSREGTTCTCKQGQLNC 726 HSP 5 Score: 53.9138 bits (128), Expect = 2.128e-6 Identity = 30/72 (41.67%), Postives = 41/72 (56.94%), Query Frame = 3 Query: 4227 TTHCVEGCSCPNGMI-DLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCT 4439 T CV GC CP+G++ D NG C+ CPC +NG SY G +K E+C C C ++ CT + C TC+ Sbjct: 775 TRGCVSGCVCPSGLVSDDNGGCITEDLCPCVHNGASYKPGETVK-EDCNTCTCKDRRWQCTTN--QCSATCS 843
BLAST of SCO-spondin vs. Ensembl Cavefish
Match: ENSAMXT00000019204.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001825.1:10421:34530:-1 gene:ENSAMXG00000018303.2 transcript:ENSAMXT00000019204.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 311.612 bits (797), Expect = 7.618e-86 Identity = 304/1192 (25.50%), Postives = 485/1192 (40.69%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAG-NIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIR--LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYT---CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIM-GTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTK----LPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFD--GSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGS-CDNTPQSIVDPPAGDND-------ICSTILN--GPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGI---QYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFP--GCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD-SVSG--------CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPL----FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM-HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK-------HYDACASPCPQTC 4190 C +G +++TFD +Y +QG C Y L + S +S+++ T D+ +V+V GD+ +K + N ++ D + +P VL + + L A + + W+ ++ + + + GLCG F G + +S + D CS++ + C +V+ + Y CV + C S+ + +P LCT LS YS CS G WR C +CP N +S+ S C TC + S C+ K+ C C G V + CI E C C ++ + + G V + C C+ W C P C + G+ F TFDG +F F+ C Y+ + Q ES G +++ + +D LK+ + + N + +D T K L NI V + ++ + V+ S ++ +YI + + + GLCG F+ ++ + + + A S+ S C + +S+ DP + + D CS +L GP F+ C +S V+ E Y C+ + K + C + AC GI + + C P Q Q C RTC S E C + P GC CP Y+ G CV+ +C+C++ I G +ITI + CVC NG+L+C + CP +V+ + S +G +C + + C CP+ + G CV +CPC + Y P IT +C C W E+ C C YG HY T D + F F G C Y + N + N+ CG + TC+K V+I++G L G L KY + + + + +D IGL VLWDR T V + L+P G V GLCGN++G+ ++++ G ++ D ++FA S+ N+ CP + + D C+ +R +WA+ C ++ T F C + D Y+ NC+ D+C C+ GGDCEC CT++A +A C G+ V WRT +CP+ CE HY C +PC +TC Sbjct: 3 VCSMWGNFHFKTFDGDVYQFQGICEYNLVSHCHGSVQDFSVHVKRSEFTGDSKINRVLVT-IGDMAIKLTKNLVMVN---------------DDVVKLPHYVAGVLLEQYATYIKLYAKLGLSVMWNKDDAVMVELEAKYT-NQTCGLCG---------------------DFNGMAI--LNEFISEAQACDYSSQRPGCSELLEDKAWKSCTSIVSPEPYIQACVVDRCW-SRPGDIEDSLP-----LCTTLSEYSRQCSHAGGTPPNWRKPDFCAVKCPYNMEYSESGSPCVDTCSH--TDTSLLCEEHKMDGCYCPPGTVFDDISDRGCIPQEQCQCKHN-KVYNSGDVLHMEDKECVCHHGSWTCESLPTPGLCAVEEGSHFTTFDGKEFTFHG-ECYYI-----------------LSQDCEESKFTVLG--------QLIPCVDHETDTCLKSVVLLPNNDKNNALVIKDDGTVKQNGDLTLPYASANIIVFRPSSFHIIVQTSFGLRMQVQLVPVMQLYITLDRSYQTKTCGLCGNFNMVLSDELKTPQGLVEGTASSFANSWKAQSNCRDRSESLDDPCSYNIDSESYAEHWCSKLLEKEGP-FAKCHSS--VNPESYYKRCKYSTCTCEKTENCLCGVFSSYVRACTAKGIFLQGWRDTVCKKYTEHC-PATQTFSYKLQGCQRTCDSLSLE-----RPACINTYLPVDGCACPDGLYENENGVCVALDKCSCYHNGE----RIKPGKSITIKNEHCVCTNGKLHCHSWKPSVMD-CPAPKVFFNCSEAGPEEHGLECAKTCAQKTVDCFLTGCTSGCQCPSGLLDDGRGHCVPQHNCPCKHNGHYYPPGSQITDQCNTCTCKQGKWKCTEEK---CPGTCTIYGSGHYNTFDNRRFGFSGDCGY--IAAQNKCGNKTGTFHVITENVPCGTTGTTCSKSVRIVLGKTQLDLIDGKVTNTDLESGTLIKYRV--RNVGLYLAIEAD-IGLTVLWDRKTTVRIILQPQHMGEVCGLCGNFNGNGKDDFTTQGQLQVSDVLEFANSWKVNSH-CPDAVP---------DFDPCSKAHNRHTWAKMQCSII--TGKTFKECHSMV--DPAPYFDNCVRDSCACDTGGDCECFCTAVAAYAQACNEAGVCVAWRTPEICPVFCEFYNDPLECLWHYSPCHTPCYKTC 1083 HSP 2 Score: 137.502 bits (345), Expect = 7.164e-32 Identity = 148/532 (27.82%), Postives = 228/532 (42.86%), Query Frame = 3 Query: 2901 CWNPSRPKDPE--IPAGTNITINCQTCVCINGRL-NCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTC--TDTSSI-----ANSCGCPNDTVSK--IDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIAC-GFSLATCAKFVQII---------IGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLR--VLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYED-AIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVN--RYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIM---CEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 CW SRP D E +P T ++ + C G N + D C CP N Y +S S C DTC TDTS + + C CP TV D C+ C C + N+ Y DV+ ++ + +C + W S T +C H+ T DG+ F+F G C Y L +S T+ I C TC K V ++ + + + + T+P + +I++ + + F +V S GLR V ++Y+ L S+Q GLCGN++ L +E E A FA S+ A ++ S + D C+ N D +S+AE C + + G FA C H VN YY C Y C C + +C LC +++ C GI ++ +C C + + C +TCD++ P+ +T V+GC+CP+G+ + NG CV C C++NG+ G I ++N + C C K +C Sbjct: 192 CW--SRPGDIEDSLPLCTTLSEYSRQCSHAGGTPPNWRKPDFCAVK-CPYNMEYSESGSPC-----VDTCSHTDTSLLCEEHKMDGCYCPPGTVFDDISDRGCIPQEQCQCKH-NKVYNSGDVLHMEDKECVCHHGSWTCESLPTP---GLCAVEEGSHFTTFDGKEFTFHGECYYILSQDCEESK-----FTVLGQLIPCVDHETDTCLKSVVLLPNNDKNNALVIKDDGTVKQNGDLTLP-YASANIIVFRPSSFHIIVQTSFGLRMQVQLVPVMQLYITLDRSYQTKTCGLCGNFNMVLSDELKTPQGLVEGTASSFANSWKAQSNCRDRSESLD---------DPCSYNIDSESYAEHWCSKLLEKEGPFAKC----HSSVNPESYYKRCKYSTCTCEKTENC--LCGVFSSYVRACTAKGIFLQGWRDTVCKKYTEHCPATQTFSYKLQGCQRTCDSLSLERPACINTYLPVDGCACPDGLYENENGVCVALDKCSCYHNGERIKPGKSITIKN-EHCVCTNGKLHC 689 HSP 3 Score: 109.768 bits (273), Expect = 1.711e-23 Identity = 100/380 (26.32%), Postives = 162/380 (42.63%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRG---NNDTIPLFPKY--DIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCE---CLCTSLATFATQCAFL-GIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTT-----HCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 VC +G+ H+KT DG ++ FQG C Y L+ S + SV F+ + V + IG L++ ND + P Y +++EQ + +L + +GL V+W++ V + L+ + GLCG+++G + FI+ A C Y S ++ C+ + K+W SC + + Y C+ D C +R GD E LCT+L+ ++ QC+ G P WR + C + C Y SPC TC SH T+ H ++GC CP G + D++ C+P C C +N + Y +G ++ ME+ K+C C + C Sbjct: 3 VCSMWGNFHFKTFDGDVYQFQGICEYNLVSHCHGSVQD-----FSVHVKRSEFTGDSKINRVLVTIGDMAIKLTKNLVMVNDDVVKLPHYVAGVLLEQYATYIKLYA-KLGLSVMWNKDDAVMVELEAKYTNQTCGLCGDFNG----------------MAILNEFISEAQACDY----------SSQRPGCSELLEDKAW---------------KSCTSIVSPE--PYIQACVVDRCW-SRPGDIEDSLPLCTTLSEYSRQCSHAGGTPPNWRKPDFCAVKCPYNMEYSESGSPCVDTC--------SHTDTSLLCEEHKMDGCYCPPGTVFDDISDRGCIPQEQCQCKHN-KVYNSGDVLHMED-KECVCHHGSWTC 322 HSP 4 Score: 80.8777 bits (198), Expect = 1.074e-14 Identity = 91/326 (27.91%), Postives = 136/326 (41.72%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAG-NIKLKWDXXXXXXXXXXXXXXXGDVTGLCGX-----XXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLSTCKP--DDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQC-----PGNKIW--SDCASECPVTCRNLFQTPSAYCQN 1685 TC YG +Y TFDN+ + + G C YI A + T + +++ EN T+ T C K + I G + L VTNTD + GT + ++ + L++EA + + WD I + G+V GLCGN D+ + +V F NS+K CPD C + A QCS I K F C +V+ YF CV++ C D + CT ++AY+ +C++ G C+ WR+ +C C P +W S C + C TC N P C+N Sbjct: 799 TCTIYGSGHYNTFDNRRFGFSGDCGYI-AAQNKCGNKTGTFHVITENVPCGTTGTTCSKSVRIVLGKTQLD---------------LIDGKVTNTD---LESGTLIKYRVRNVGLYLAIEADIGLTVLWD-RKTTVRIILQPQHMGEVCGLCGNFNGNGKDDFTTQGQLQVSDVLEFANSWKVNSHCPDAVPDFDPCSKAHNRHTWAKMQCSIITGKTFKECHSMVDPAPYFDNCVRDSC--------ACDTGGDCECFCTAVAAYAQACNEAGVCVAWRTPEICPVFCEFYNDPLECLWHYSPCHTPCYKTCLN----PDGICRN 1092 HSP 5 Score: 57.7658 bits (138), Expect = 1.140e-7 Identity = 33/74 (44.59%), Postives = 41/74 (55.41%), Query Frame = 3 Query: 4221 CSTTHCVEGCSCPNGMID-LNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCT 4439 C T C GC CP+G++D G CVP CPC +NG Y G+ I + C C C Q K+ CTE+ C TCT Sbjct: 731 CFLTGCTSGCQCPSGLLDDGRGHCVPQHNCPCKHNGHYYPPGSQI-TDQCNTCTCKQGKWKCTEE--KCPGTCT 801
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010519.1 (pep scaffold:Pmarinus_7.0:GL477612:23664:47127:1 gene:ENSPMAG00000009527.1 transcript:ENSPMAT00000010519.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 305.064 bits (780), Expect = 1.908e-85 Identity = 285/1020 (27.94%), Postives = 436/1020 (42.75%), Query Frame = 3 Query: 1506 CTHLSAYSDSCSDKG-SCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEY------GYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSA---------YINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIY--TEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKD--NSIRESIVNIAKSYITGSCDNTPQSIVDPPA---GDNDI----CSTILN--GPEFSGCMNSSKVDKEFYATYCRREQSS-NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNI----LQACIPLCQAGGTVFQICS----RTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD----SVSGCNSCTNYDTCTDTSSI------ANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMC------EGG--KHYDACASPCPQTCDTIGSNAPSHCST-THCVEGC--SCPNGMIDLNGECVP-PTTCPCFYNGQSY-VNGAIIKMENCK 4370 C ++ +S C+ S WRS C CP ++ +C S C TC N + S C ++ V C C G G CI +C C GQ + G C CTC W+C + C TC I+G + F TFD + F+ C Y TD N F ++ T+ EG + YI+ + V K +G ++ H K+P ++ + + ++ Y E ++ +YI + A N GLCG F+ + +DFT ++ + N+ K+ T C + + +DP + +N+ C +++ GP FS C + D+ Y T C+ + S + + C+A+ A AC G+ V + S+I + C P+ C RT + CR + GC C Y G CV +S+C C+ S I AG + ++ C+C G L+C + C Y + S G +C TC + SI + C CP + +G CV C C + R Y P D IT C K C N W+ SE C+ +C YG+ H+ T DG+ + F GSC Y L D+ G+SS + I+ N CG + TC+K V G +L+ G L FP + ++ +T +V S+G+ ++WD T V + + P +G V GLCG+YDG + N+++ T A +FA + + CP S+ D C N R+SWA + C V+KD GVF C + + YY+ C+ D+CGC+ GGDC CLCT+LA++A C G+ ++WRT +LCP+ C EG HY C C ++C +N + C+ +EGC +CP+ LN + C C NG +Y VN I + C+ Sbjct: 213 CATIAEFSRRCTQANESPGTWRSPEFCGVTCPRGQVHRECGSVCKDTCSN--PSASVNCPSTCVDGCFCPEGTAGTVWDDVSGSGCIPQSNCGCNQLGQSYASGESLITGCKNCTCEQGQWNCDSRPCYGTCKILGGSYFSTFDSKTYRFHG-DCFYTLT------TDCAGNKFSVLGELTK----CEGRDSKTCLNSVIFYIDGIRTVVFKASGDVLV---------------------HENKVPLPFVSASVTIVQESSFYLRAETNFGLRLRVQLKPLMQLYITAATAYHNKTCGLCGNFNEVTGDDFTHSGIVDATAATFCNVWKAQAT--CPDREEVTMDPCSVYITNNEYAKAHCGLLMDNDGP-FSTCHSVVSADE--YYTRCKYDTCSCSYPEPCLCAALSAYAYACSAHGVVL-VGWRSDICSLDAENC-PVSHEFSYAAPECGSASCRTSREVCRGVRPPPFELPSPSPVEGCVCGAGLYDDEHGVCVLKSDCPCY--SEMSGDLIRAGVTVDLHDTQCLCTGGELHCFGLSPISGNTCEGLMTYHNCSDGSSEGSAACQR--TCANPHSICYSPGCVSGCVCPGGLIVDHNGNCVSEEQCTCEHNGRHYDPGDTITQLCNKCTCMNARWSCTSEV---CQGLCFVYGEGHHTTFDGKQYVFDGSCQYILSQDSCGNSSGSF---RITTENEPCGTTGTTCSKKVHFYAGADELVLTDGTVKGSSLDFPGSPFHVRRMGIYT-VVETSLGVTIIWDGHTAVSVHVNPQLKGRVCGLCGDYDGKVANDFVTRTGSVAASAPEFAAGWKESGSGCP---------DLSRVSDPCTINHHRESWAHKQCSVLKD--GVFKQCHNMV--EYLPYYAACVRDSCGCDTGGDCHCLCTALASYAQACGSAGVCLRWRTPDLCPLGCDLYNKDEGQCEWHYRECGRGCQRSC----TNRNAICTANLPPLEGCYPTCPSDRPFLNESSMTCVERCDCVSNGTTYKVNDRIPSNDPCE 1163 HSP 2 Score: 86.2705 bits (212), Expect = 5.535e-17 Identity = 98/397 (24.69%), Postives = 163/397 (41.06%), Query Frame = 3 Query: 3315 VCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDS-SNNIPPITISVANIACGFSLATCAKF----VQIIIGGQTYLLSR------GNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLY-DACGCNRGGDCECL----CTSLATFATQCAFLG-IPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI-----DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC 4436 +C +G H T D + MD P S + N+ IT S +++ F + + V + + G + L+R G +P + + I IE + + + S +G +LWD++ + + L P F GLCG+++G + Y++ + ++ F SF+ D P ++ E ++ SC+ M + N L+ D C + G C ++A F+ +C P WR+ C + C G+ + C S C TC +A +C +T CV+GC CP G D++G C+P + C C GQSY +G + + CK C C Q ++NC D C TC Sbjct: 1 ICSVWGTSHLLTFD-----------HASMDLPLSSCALNLVSITDS-PSLSLSFQRSALDRMSVENVVVTMEGISLSLTREGQSISGQRVQLP-YKDHSIQIEMVGSYIK-CSSIMGFSLLWDQNNAITVKLDPKFNTKTTGLCGDFNGIADDFYLN--VMSTTSVLF--SFLLTCD------EPTQHIEHLFEDEL-------------------------LSCVSVMDHQLKSLLVNILWSDICRLDCNGTAGLYPCDGCATIAEFSRRCTQANESPGTWRSPEFCGVTCPRGQVHRECGSVCKDTCSN--PSASVNCPST-CVDGCFCPEGTAGTVWDDVSGSGCIPQSNCGCNQLGQSYASGESL-ITGCKNCTCEQGQWNC--DSRPCYGTC 342 HSP 3 Score: 81.6481 bits (200), Expect = 1.183e-15 Identity = 78/288 (27.08%), Postives = 130/288 (45.14%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPS-DVSAVTWSINIVYENC-TSDTGCVKVIVIEAG-DVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK--GFGCPDIANTLSTCKPDD--QILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQC 1595 C YG ++ TFD + Y + G C YIL+ S S+ ++ I E C T+ T C K + AG D +V + G T S+L PG+P +V R + ++ G + + WD + + + ++ + G V GLCG A++ + S + F +K G GCPD++ C + + A++QCS ++ F C ++V Y++ CV++ C D LCT L++Y+ +C G C++WR+ LC C Sbjct: 818 CFVYGEGHHTTFDGKQYVFDGSCQYILSQDSCGNSSGSFRITTENEPCGTTGTTCSKKVHFYAGADELVLTDG------------------TVKGSSLDFPGSPFHVRRMGIYTVVETSLG-VTIIWDGHTAVSVHVNPQLK-GRVCGLCGDYDGKVANDFVTRTGSVAASAPEFAAGWKESGSGCPDLSRVSDPCTINHHRESWAHKQCSVLKDGVFKQCHNMVEYLPYYAACVRDSCG--------CDTGGDCHCLCTALASYAQACGSAGVCLRWRTPDLCPLGC 1077 HSP 4 Score: 68.5514 bits (166), Expect = 1.284e-11 Identity = 115/437 (26.32%), Postives = 171/437 (39.13%), Query Frame = 3 Query: 762 FTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCT---SDTGCVKVIVIEAG--DVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXV----ASFGNSFKGFG-CPDIANTLSTCKPDDQILANQQCSKIRL--------KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSC-IQWRSD--TLCETQCPGNKIWSDCASECPV--------TCRNLFQTPSAYCQNSKVGRCICGSG--EVEYGYTCIKPESCPCLYD--GQEHPPGTVRQFDCNTCTCNSSLWHC--TEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEP 1967 + TC G Y+ TFD++ Y + G C Y LT +D + +S+ C S T VI G VV K+SG+ +++ P SVT I+ + Y+ + NF L ++LK + Y+ GLCGN + +DF++ V A+F N +K CPD T P + N + +K F C +V++D Y++ C + C+ S LC LSAY+ +CS G + WRSD +L CP + +S A EC CR + P S V C+CG+G + E+G C+ CPC + G G C C HC I +TC + T + DG+ + CQ P Sbjct: 339 YGTCKILGGSYFSTFDSKTYRFHGDCFYTLT--TDCAGNKFSVLGELTKCEGRDSKTCLNSVIFYIDGIRTVVFKASGDVLVHENKVPLPFVSASVT------IVQESSFYLRAETNFGLRL----RVQLK---PLMQLYITAATAYHNKTCGLCGNFNEVTGDDFTHSGIVDATAATFCNVWKAQATCPDREEV--TMDPCSVYITNNEYAKAHCGLLMDNDGPFSTCHSVVSADEYYTRCKYDTCSCSYP----------EPCLCAALSAYAYACSAHGVVLVGWRSDICSLDAENCPVSHEFSYAAPECGSASCRTSREVCRGVRPPPFELPSPSPVEGCVCGAGLYDDEHG-VCVLKSDCPCYSEMSGDLIRAGVTVDLHDTQCLCTGGELHCFGLSPISGNTCEGLMTYHNCSDGS--SEGSAACQRTCANP 745 HSP 5 Score: 55.8398 bits (133), Expect = 9.789e-8 Identity = 41/109 (37.61%), Postives = 55/109 (50.46%), Query Frame = 3 Query: 4119 NLCPI---MCEGGKHYDACA-------SPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI-DLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED 4412 L PI CEG Y C+ + C +TC +N S C + CV GC CP G+I D NG CV C C +NG+ Y G I + C KC C+ A+++CT + Sbjct: 709 GLSPISGNTCEGLMTYHNCSDGSSEGSAACQRTC----ANPHSICYSPGCVSGCVCPGGLIVDHNGNCVSEEQCTCEHNGRHYDPGDTIT-QLCNKCTCMNARWSCTSE 812 HSP 6 Score: 50.8322 bits (120), Expect = 3.095e-6 Identity = 73/351 (20.80%), Postives = 142/351 (40.46%), Query Frame = 3 Query: 1980 TDDRANVFVIIQSNTESMLG-TEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKT------GTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTK 3011 T D A++ + + S +++ T+ S ++ + +++ ++++ L ++ I G+ +LP+ D +I + ++ Y + F L +D + +I +K+ GLCG F+G +++ + S ++ S++ +CD Q I + C ++++ S +N ++ CR + + L C A RR E P T + C C G V + C C+ TC N + + C C GC CP T G+G C+ +S C C + +G ++ C+ C C G+ NC ++ Sbjct: 12 TFDHASMDLPLSSCALNLVSITDSPSLSLSFQRSALDRMSVENVVVTMEGISLSLTREGQSISGQ--RVQLPYK--DHSIQI-EMVGSYIKCSSIMGFSLLWDQNNAITVKLDPKFNTKTTGLCGDFNGIADDFYLNV-----MSTTSVLFSFLL-TCDEPTQHIEHLFEDELLSCVSVMDHQLKSLLVNI------LWSDICRLDCNGTAG-LYPCDGCATIAEFSRRCTQANESPGTWRSPEFCGVTCPR-GQVHRECGSVCKDTCS--NPSASVNCPSTCVDGCFCPEGTAGTVWDDVSGSG-CIPQSNCGCNQLGQ----SYASGESLITGCKNCTCEQGQWNCDSR 336
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008773.1 (pep scaffold:Pmarinus_7.0:GL476328:703641:749596:1 gene:ENSPMAG00000007891.1 transcript:ENSPMAT00000008773.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 298.901 bits (764), Expect = 6.082e-82 Identity = 268/1002 (26.75%), Postives = 425/1002 (42.42%), Query Frame = 3 Query: 1332 PDDQILANQQCSKI--RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSC-SDKGSCIQWRSDTL---CETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYT---CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFID--GE----DHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDP-------PAGDNDICSTIL--NGPEFSGCMNSSKVDKEFYATYCRREQSSNKD-QLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDD--CFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMD---SVSGCNSCTNYDTCTDTS----SIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIV----IEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGT-MKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDM-CNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEG-------GKHYDACASPCPQTC 4190 PDD + C + +F C ++ Y C+ + C+ T D P V YS C S G+ WR+ + +C I+ +C CP +C + S C V C C G + T CI E C C+++ + G + DC C C+ +W+C E+ C TC + G + TFD +F F+ ++A+ D R ++F ++ + + LK+ + LG + + I GE D LP+ I + I+ + + + +Y+ + + GLCG ++G +DF + I + + A S+ +C +S +P D+CS + +GP FS C + V E + C + S +D + C+A+ + A+AC + E + ++ C G V+ + C +TCR L E T S+C GC CP Y +G G C++ EC C+ + +P G ++ C C G + C T CP VY + S + NS + TC+ + C CP V DGTC+QP CPC + ++ AP + I + C C NR W + C + C YG HY+T DGQ F F+GSC Y + + NN I+ N+ CG + TC++ + + +G L + + L + + I+ + +V S + + WD++T V++ L P ++G V GLCGN+DG N+ T M ED ++F S+ + +C A +KE C R WA+Q CG++K + FASC + DV ++ C++DACGC+ GGDCECLC ++A +A C G +KWRT +LCP+ CE HY C CP+TC Sbjct: 175 PDDCSMHESLCELVLNTPEFADCKARLDVRPYIGACIVDQCS------CTSDCPDFGTV-----EEYSRECRSIGGTPGNWRNTSETCGAAPECLPGTIYQECGIPCPNSCSQ--PSLSNMCIEECVPGCFCPRGTIWDDLTDKGCIPLEECSCVFNKHTYNAGDILHQDCQDCVCHGGVWNCEEQPCTRTCALEGGSHIKTFDQNQFTFHGDCYYFMAM-------DCRTHLFNLM---------------------VKLKPCETKNTCLKSLALILGRNVLKIMISDSGEVSVNDVEVNLPYDSNAITIFYQSSFYIHLHTSIGVNLLVQLSPVMQLYLTLDPSFHTATCGLCGDYNGIQSDDFKTSNGLIEGTAIYFANSWKAQNTCPPPTESTEEPCMLSLNHKQYAEDMCSNMKTPDGP-FSACHGT--VSPEAFYKRCLYDTCSCQDSEACLCAALSSYALACASKHVVLE-GWREHLCAKYTENC-PGSQVYSYNALPCVNTCRVLQE-TGSQCFSSGVAVDGCTCPKGTYDSGGGFCMAPEECPCYY----RGNVLPLGNSVQQGKYICECRAGTMLCMNPQGPTPQPCPHPMVYFNCNQSTANGNSFPCHQTCSLQECYNPECNSGCVCPKGLVYSDDGTCIQPEACPCEHNGQKTAPGESIEVDCNTCTCVNRKWECTNN---TCLSTCSLYGQGHYRTFDGQRFLFEGSCTYVIAQDNCQNPNNEGTFNITTENVPCGTTGTTCSRSINVQVGNWVIKLFQKSYSIDFLGSGPETINPFQIKHRGLYL-VVETSNRITLTWDKNTGVFISLGPEYKGTVCGLCGNHDGRAANDLTTRTHMVVEDPLEFGNSWKLSG-LC----------ADAKETSSPCALMSHRLPWAQQRCGILK--SDTFASCHALV--DVLPFHEACVHDACGCDTGGDCECLCNAVAVYAKACNEAGQCIKWRTPDLCPLFCEYYDAPDDCTWHYRPCGQSCPKTC 1106 HSP 2 Score: 146.362 bits (368), Expect = 3.429e-35 Identity = 118/413 (28.57%), Postives = 178/413 (43.10%), Query Frame = 3 Query: 3129 CGCPNDTV--SKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATC--AKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRS--TRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYED-AIQFAESFIANADICPYEISPENNVAPSKE--QDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIM---CEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGE-CVPPTTCPCFYNG 4325 C CP T+ D C+ +C C++ Y D++ C +C +WN E C C G H KT D F+F G C Y + MD N + + C SLA ++I+I + +P + Q +F+ L + SIG+ +L S ++YL L PSF GLCG+Y+G +++ E AI FA S+ A +N P E ++ C + + K +AE MC MK +G F++C + + +Y CLYD C C CLC +L+++A CA + ++ ++LC C G + Y A PC TC + S+ V+GC+CP G D G C+ P CPC+Y G Sbjct: 291 CFCPRGTIWDDLTDKGCIPLEECSCVFNKHTYNAGDILHQDCQDCVCHGGVWNC---EEQPCTRTCALEGGSHIKTFDQNQFTFHGDCYYFMAMDCRTHLFNLMVKLKPCETKNTCLKSLALILGRNVLKIMISDSGEVSVNDVEVNLPYDSNAITIFYQSSFYIHLHT-SIGVNLLVQLSPVMQLYLTLDPSFHTATCGLCGDYNGIQSDDFKTSNGLIEGTAIYFANSWKA-----------QNTCPPPTESTEEPCMLSLNHKQYAEDMCSNMKTPDGPFSACHGTVSPEA--FYKRCLYDTCSCQDSE--ACLCAALSSYALACASKHVVLEGWREHLCAKYTENCPGSQVYSYNALPCVNTCRVLQETGSQCFSSGVAVDGCTCPKGTYDSGGGFCMAPEECPCYYRG 684 HSP 3 Score: 109.768 bits (273), Expect = 4.714e-24 Identity = 108/414 (26.09%), Postives = 174/414 (42.03%), Query Frame = 3 Query: 1701 CICGSGEV-EYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDN-----TPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFG--IQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCD--DDCFPGCECPPNYYKTGTGQCVSESEC 2897 C+C G V TCI+PE+CPC ++GQ+ PG + DCNTCTC + W CT C STC + G + TFDG +F F + C YV + + N ++ F I N GT G + + + V+V G+ +I +L ++ G T PF I K +Y V+ S+ L +D + ++I + K + GLCG DG + ND T + + + E + S+ ++G C + +P +++ IL F+ C + VD + C + + D C+A+ A AC G I++ P + ++ C ++C TCR+ + + C+P C Y+ CVS C Sbjct: 759 CVCPKGLVYSDDGTCIQPEACPCEHNGQKTAPGESIEVDCNTCTCVNRKWECTNNTCLSTCSLYGQGHYRTFDGQRFLF-EGSCTYVIAQDNCQNPNNEG-TFNITTENVP--CGTTGTTC----SRSINVQV-GNWVI------KLFQKSYSIDFLGSGPETINPFQI--------KHRGLYLVVETSNRITLTWDKNTGVFISLGPEYKGTVCGLCGNHDGRAANDLTTRTHMVVEDPLEFGNSWKLSGLCADAKETSSPCALMSHRLPWAQQRCGILKSDTFASC--HALVDVLPFHEACVHDACGCDTGGDCECLCNAVAVYAKACNEAGQCIKWRTPDLCPLFCEYYDAPDDCTWHYRPCGQSCPKTCRDPHAECREGVQRFEGCYPKCTESKPYFDEDNMLCVSWDSC 1147 HSP 4 Score: 104.76 bits (260), Expect = 1.612e-22 Identity = 106/381 (27.82%), Postives = 164/381 (43.04%), Query Frame = 3 Query: 3318 CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITI-SVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFL-GIPVKWR-TQNLCPIM--CEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC 4436 C +G H+KT DG+ F SC Y D++ N I SV N S+ + I + + +++ + D +P F +Y I I L ++ + +IG ++ W++ + L + S++ GLCG+Y+GD + G + + AE + N P + P+ PD S E +C ++ +T FA C + DV Y C+ D C C DC T + ++ +C + G P WR T C C G Y C PCP +C + S+ CV GC CP G I DL + C+P C C +N +Y G I+ ++C+ C C +NC E C TC Sbjct: 1 CSTWGQNHFKTFDGKFFDLGISCNYLFASNCKDATPNFNVIIRRSVNNPQIISSINISIIDINIDLSAEGAAVNKESQD-LP-FNQYGIQIIALGDDVKITATNIGFKLFWNKKDSLLLQVTESYRNKTCGLCGDYNGDAEYPEFKGGI-----LSVAEYALNNKYYDPMVQCDQIEQPPT---------PDDCSMHESLCELVLNTPE-FADCKARL--DVRPYIGACIVDQCSCT--SDCPDFGT-VEEYSRECRSIGGTPGNWRNTSETCGAAPECLPGTIYQECGIPCPNSC---SQPSLSNMCIEECVPGCFCPRGTIWDDLTDKGCIPLEECSCVFNKHTYNAGDILH-QDCQDCVCHGGVWNCEE--QPCTRTC 353 HSP 5 Score: 83.9593 bits (206), Expect = 3.171e-16 Identity = 120/478 (25.10%), Postives = 180/478 (37.66%), Query Frame = 3 Query: 477 GGICMSPNQCKC-------------QENYAGMQCDEDNPIIIQSQHPSQLYC------YQSDQCFAFKKSDFLGKAVSYDEC--------CADKTGSWGSQSGSCVSCKA--------NFAGGNDV-----------------NNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTA-----PSDVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVA-----SFGNSFKGFG-CPDIANTLSTCKPDDQIL--ANQQCSKIRL-KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQC-----PGNKIW--SDCASECPVTCRNLFQTPSAYCQ 1682 GG CM+P +C C Q+ +C + + Q P+ C + +Q A S + S EC C G S G+C+ +A A G + NN +TC YG +YRTFD Q + ++G C+Y++ P++ + NI EN T+ T C + I ++ G+ V+K + P T I P + ++ L+ + I L WD N Y G V GLCGN D +ND + ++ FGNS+K G C D T S C L A Q+C ++ F C LV+ + CV + C D + LC ++ Y+ +C++ G CI+WR+ LC C P + W C CP TCR+ P A C+ Sbjct: 669 GGFCMAPEECPCYYRGNVLPLGNSVQQGKYICECRAGTMLCMNPQGPTPQPCPHPMVYFNCNQSTANGNSFPCHQTCSLQECYNPECNSGCVCPKGLVYSDDGTCIQPEACPCEHNGQKTAPGESIEVDCNTCTCVNRKWECTNNTCLSTCSLYGQGHYRTFDGQRFLFEGSCTYVIAQDNCQNPNNEG----TFNITTENVPCGTTGTTCSRSINVQVGNWVIKLFQKSYSIDFLGSGPET-----------INP----FQIKHRGLYLVVETSNRITLTWD-KNTGVFISLGPEYKGTVCGLCGNHDGRAANDLTTRTHMVVEDPLEFGNSWKLSGLCADAKETSSPCALMSHRLPWAQQRCGILKSDTFASCHALVDVLPFHEACVHDAC--------GCDTGGDCECLCNAVAVYAKACNEAGQCIKWRTPDLCPLFCEYYDAPDDCTWHYRPCGQSCPKTCRD----PHAECR 1114 HSP 6 Score: 75.8702 bits (185), Expect = 8.950e-14 Identity = 93/387 (24.03%), Postives = 158/387 (40.83%), Query Frame = 3 Query: 1896 QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSS---HFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSE-NDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDN-----DICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQ----YEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPN--YYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTK 3011 F TFDG F + C Y+ N D N VII+ + +N P+I++ I S + ++ E LPF N + ++ + +VK+++ FKL+++ S+ ++V+++ +N GLCG ++G++E +F S+ E +N CD Q PP D+ +C +LN PEF+ C +++D Y C +Q S + + CR G T C+P GT++Q C C ++C + + ++ C ++C PGC CP + C+ EC+C + AG + +CQ CVC G NC+ + Sbjct: 8 HFKTFDGKFFDLG-ISCNYLFAS---NCKDATPNFNVIIRRS-------------VNNPQIISSINISIIDINIDLSAE------GAAVNKESQ--DLPF-----NQYGIQIIALGDDVKITATNIGFKLFWNKKDSLLLQVTESYRNKTCGLCGDYNGDAEYPEFKGGILSVAEYALNNKYYDPMVQCDQIEQ----PPTPDDCSMHESLCELVLNTPEFADC--KARLDVRPYIGACIVDQCSCTSDCPDFGTVEEYSRECRSIGGTPGNWRNTSETCGAAPECLP-----GTIYQECGIPCPNSCSQ--PSLSNMCIEECVPGCFCPRGTIWDDLTDKGCIPLEECSCVFNKHTYN----AGDILHQDCQDCVCHGGVWNCEEQ 347 HSP 7 Score: 72.4034 bits (176), Expect = 9.619e-13 Identity = 94/389 (24.16%), Postives = 160/389 (41.13%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVT-GLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG-CPDIANTLSTCKP-----DDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCET---QCPGNKIWSDCASECPVTCRNLFQTPSA-YCQNSKVGRCICGSGEVEYGYT-CIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPS 1874 TC G + +TFD +T+ G C Y + D +++ + + C + C+K + + G V+K + +DS +SV + + L + + Q +F + + + L + + + + T GLCG+ + S+DF + + F NS+K CP T ST +P + + A CS ++ F C V+ + ++ C+ + C+ Q+S LC LS+Y+ +C+ K ++ + LC CPG++++S A C TCR L +T S + V C C G + G C+ PE CPC Y G P G Q C C + C P+ Sbjct: 352 TCALEGGSHIKTFDQNQFTFHGDCYYFMAM--DCRTHLFNLMVKLKPCETKNTCLKSLALILGRNVLK------IMISDSGE----VSVNDVEVNLPYDSNAITIFYQSSFYIHLHTSIGVNLLVQLSPVMQLYLTLDPSFHTATCGLCGDYNGIQSDDFKTSNGLIEGTAIYFANSWKAQNTCP--PPTESTEEPCMLSLNHKQYAEDMCSNMKTPDGPFSACHGTVSPEAFYKRCLYDTCS-CQDSEA---------CLCAALSSYALACASKHVVLEGWREHLCAKYTENCPGSQVYSYNALPCVNTCRVLQETGSQCFSSGVAVDGCTCPKGTYDSGGGFCMAPEECPCYYRGNVLPLGNSVQQGKYICECRAGTMLCMNPQGPT 716
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008716.1 (pep scaffold:Pmarinus_7.0:GL476328:595486:663084:-1 gene:ENSPMAG00000007851.1 transcript:ENSPMAT00000008716.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 286.189 bits (731), Expect = 6.043e-78 Identity = 273/1016 (26.87%), Postives = 427/1016 (42.03%), Query Frame = 3 Query: 1506 CTHLSAYSDSCSDKGSCI-QWRSDTLCETQ-CPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGY---TCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGI-MGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKS--IYIKVSDALKNN-----LIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGDNDI-------CSTILNGPE-FSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGIQYE--VPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCF--PGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC--KTKDNCTNPVCPKNQVYMDSVSGC---NSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKY-----DIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNR--YYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACA---SPCPQT-CDTIGSNAPSHCSTTHCVEGC--SCPNGMIDLNGE---CVPPTTCPCFYNGQSY-VNGAIIKMENCKKCQCVQAKFNC 4403 C LSAYS CS + + WRS +C Q CPGN + +C S CP TC N+ PS CQ V C+C SG V TCI PE C C Y+G + DCN+CTC + W CT ++CP C + G F+TFD + F+ V Y+AV+ V +++ + + + N + + +T K + K+ N I + LP+ ++ + + ++++ +V S + S + +YI + D++ GLCG F+G + +DF + + A S+ + C++ I DP + + C+ + N F C VD Y + C+++ S Q L+ A+AC G+ +E T + L C V+ + C TCR L+ + C + GC+C + G CV C C+ P G +TI C C G+L C K++ +P P V + ++S N C C CP ++ +G CV S C ++ + + Y C + WN + C++ C YGD HY T DGQ F F G+C Y + S + I NI CG S++TC+K ++ + + L+ G + + ++ +V S G++++WDR T +++ P Q + GLCG Y+ D+ +++ M + F S+ A +D C ++V + E C NP RK+W+E+ C ++K VF +C H V+ +Y C+ D+C CN GGDCEC CTS+A +A C G V WRT +LCP+ C+ D+C PC T G+ A +EGC CP L+ CV C C Q NG ++ + C+ C CV C Sbjct: 230 CAALSAYSRECSLQYKPLPNWRSSQMCPPQDCPGNLEYQECGSTCPPTCANM--DPSK-CQEPCVAGCVCPSGTVLSAINEETCIAPEECRCNYNGITYDASDKITIDCNSCTCEAGSWTCTTRLCPKACSLESGAHFNTFDSRSYTFHGVCSYYLAVK----------QAHVALRALGQKLECSAKNPSDLGSISSLTDKKRIRSLEFKS----------NGKIISSQMSVSLPYI--SDSVKIFRKSSMFIQVDTSYGMMMLVQNSPTMQVYIGLIDSVMFRETVCTPTGLCGNFNGIASDDFMSSQGLVEGTAAAFANSWKVNPDCEDVGTVIRDPCQLNTAVAKQAELRCAILRNSTAFFKDCHY--LVDPYSYYSRCKQDACSCPSGQDCITETFLSYALACASKGLIFENWRKATDSQLVEC-----PNAEVYSYNANACNQTCRSLSSVDLA-CQESGLVAEGCQCAEGLFANDEGMCVPAVSCPCYVNGDILKP----GEKVTIADIPCYCREGQLLCSESKKEDHVDPY-PPPPVAIQNISTLVRINECVVLYFLQLGRQCQPGCICPEGLINNGNGDCVNNSPCSVCHLVMS-GRKEQCHCGASVYKCYSGSWNCTELQ---CDSTCKMYGDGHYTTFDGQRFQFDGNCEYVVAQDFCVSDPKVGTFQIITENIPCG-SMSTCSKSIRFFLQNKEIKLADGKYEIHDVSGSASKGSEEVFTVHTVGLYLIVKASNGIKLIWDRRTSLFIIAPPDKQNQLCGLCGKYNFDMSDDFTMRDHSLSASPLHFGNSWKA-SDAC-------SDV--TDEASPCEMNPYRKAWSERKCSILK--TDVFDAC----HSQVDHIPFYEACVRDSCACNIGGDCECFCTSVAAYAEACTSAGQCVAWRTPDLCPVFCDYYNSPDSCTWHYRPCGIVNTKTCGNPAGIDLGNFPQLEGCYPKCPAKTPYLDEHMMTCVSMDKCSCEMGSQIVPANGTVVNRDTCETCTCVMGNMEC 1186 HSP 2 Score: 90.1225 bits (222), Expect = 4.639e-18 Identity = 109/405 (26.91%), Postives = 163/405 (40.25%), Query Frame = 3 Query: 3297 TANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN--NDTIPLFPKYDIVIEQLTFFTQLVSDSIGLR--VLWDRSTRVYLFLKP-----SFQG--------------NVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANAD-ICPYEISPE-NNVAPSKEQDMCNANPDRKSW----AEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWR--TQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGE---------CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQA 4391 T C C +GDPHY+T DGQ ++F C YTL++ I SV F T A FV+ I + GN N ++P VI ++ F Q V+ + V+ + T++ + + SF G N QG CG + ++ M + EDA A + AD + P P AP+ ++ + P + + +C ++ +N FA C EA D++ ++ +C+YD C E C SL A QCA LG V WR T+ +C C G Y +CD G+ T+ EGC CP GM N + C+ P P + G S+ +CK+C C +A Sbjct: 1952 TWKCNCQCQGWGDPHYRTFDGQKYTFLEDCTYTLVE------EKINKYAFSVLVDNYYFYKQTPASFVRGIT-----VNYNGNSANISLPAAKGRQTVINKVIFNGQAVAFPFVGKGFVITNHETQILVEIPAIGASVSFTGMMFTVDLPYSLFGNNTQGQCGTCNNARGDDCMRQSGVVEDASCCA---VTAADWMVPDASKPHCRAKAPTGQKPCPSTTPTTRPYCTADTATICDIIT-SNRAFAGCAEAT--DLSEFFDSCVYDTCAVPTA--LEVGCASLQVAAEQCAALGFYVDWRSLTRGMCQYNCSSGFEYQPIGPKEQLSCDYSGATI----QTSILEEGCFCPKGMKMFNSQAQTCVVECGCMGPQGKPVMF-GNSW-------QSDCKECVCDKA 2325 HSP 3 Score: 81.6481 bits (200), Expect = 1.719e-15 Identity = 96/379 (25.33%), Postives = 149/379 (39.31%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNND--------TIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIM-CEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DLNGE-CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCT 4406 +AVC +G +KT D F F +C Y D ++ I +++ A +F +I+ T + N T+P F I+I+ +L + + + + F+ + GLCGN+DG + +K SF A YE+ V S+ P + + Q K T V +SC + + Y + C + C C G C C +L+ ++ +C+ P+ WR+ +CP C G Y C S CP TC + PS C CV GC CP+G + +N E C+ P C C YNG +Y I ++ C C C + CT Sbjct: 3 QAVCTTWGGGFFKTFDEAFFKFSSNCSYMFFQ---DCRSSYEEFNIKISHGAN-------EQFSEILFKIDTDVAQFANGKVSFNNRILTLP-FSNNKILIQTHGSGVKLAFRQLEISAYLNDDA-FTVVAGDEFRQQLCGLCGNFDGSSTVTTSYSRLK------MLLSFSA------YEV--LFFVKCSQLHLNLKTLPAIQQYLIQPKSSGKCTAVVTSSCKGSCGLNQEGYAAMCAIEKCQC--GSATTCGCAALSAYSRECSLQYKPLPNWRSSQMCPPQDCPGNLEYQECGSTCPPTCANMD---PSKCQEP-CVAGCVCPSGTVLSAINEETCIAPEECRCNYNGITYDASDKITID-CNSCTCEAGSWTCT 348 HSP 4 Score: 60.8474 bits (146), Expect = 3.740e-9 Identity = 91/376 (24.20%), Postives = 157/376 (41.76%), Query Frame = 3 Query: 792 YYRTFDNQLYTYQGRCSYILT---APSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIANTLS-TCKPDDQIL--ANQQCSKIR---LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCE-TQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGR--CICGSGEVEYGY-TCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTE 1859 ++ TFD++ YT+ G CSY L A + A+ + +N SD G + + + ++ NG + ++ + L +S DS I + M++ ++ ++ L + ++ + S+ TGLCG +D+ S+ A+F NS+K C D+ + C+ + + A +C+ +R F C LV+ +Y+S C Q+ C+ PS D + +Y+ +C+ KG + WR T + +CP +++S A+ C TCR+L A CQ S + C C G C+ SCPC +G PG C C C+E Sbjct: 363 HFNTFDSRSYTFHGVCSYYLAVKQAHVALRALGQKLECSAKN-PSDLGSISSLTDKKRIRSLEFKSNGKIISSQMSVSLPYIS----DSVKIFRKSSMFIQVDTSYGMMMLVQNSPTMQ--VYIGLIDSVMFRETVCTPTGLCGNFNGIASDDFMSSQGLVEGTAAAFANSWKVNPDCEDVGTVIRDPCQLNTAVAKQAELRCAILRNSTAFFKDCHYLVDPYSYYSRCKQDACS----------CPSGQDCITETFLSYALACASKGLIFENWRKATDSQLVECPNAEVYSYNANACNQTCRSLSSVDLA-CQESGLVAEGCQCAEGLFANDEGMCVPAVSCPCYVNGDILKPGEKVTIADIPCYCREGQLLCSE 720 HSP 5 Score: 60.077 bits (144), Expect = 5.989e-9 Identity = 78/331 (23.56%), Postives = 127/331 (38.37%), Query Frame = 3 Query: 2100 SDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKN------IHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEK------DNSIRESIVNIAKSYITGSCDNTP---QSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQ--CVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPK 3041 S+I+ K D+ F +G+ T+P N H + + ++++S++ + + D + L GLCG FDG+S + S E + + S + + P Q ++ P + C+ ++ S C S +++E YA C E+ + A L+A R +QY+ Q C P G +Q C TC TC ++ SKC + C GC CP + + C++ EC C D A ITI+C +C C G C T+ +CPK Sbjct: 56 SEILFKI------DTDVAQFANGKVSFNNRILTLPFSNNKILIQTHGSGVKLAFRQLEISAYLN-----DDAFTVVAGDEFRQQLCGLCGNFDGSSTVTTSYSRLKMLLSFSAYEVLFFVKCSQLHLNLKTLPAIQQYLIQPKSSGK--CTAVVT----SSCKGSCGLNQEGYAAMCAIEKCQCGSATTCGCAALSA--YSRECSLQYKPLPNWRSSQMCPPQDCPGNLEYQECGSTCPPTCANMD---PSKCQEPCVAGCVCPSGTVLSAINEETCIAPEECRCNYNGITYD----ASDKITIDCNSCTCEAGSWTCTTR------LCPK 354
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008652.1 (pep scaffold:Pmarinus_7.0:GL477625:577100:586386:1 gene:ENSPMAG00000007836.1 transcript:ENSPMAT00000008652.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 264.233 bits (674), Expect = 1.359e-75 Identity = 191/671 (28.46%), Postives = 314/671 (46.80%), Query Frame = 3 Query: 2247 EVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-----ITGSCDNTPQSIVDPP--------AGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTG-QCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNP--VCPKNQVYMDSVSGCN-SCTNYDTCTDTSSIANSC--GCPNDTVSKIDGT-CVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDT--------------PGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLF-------PKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ--FAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLC 4127 +V + + ++ +DG + +Y+ L+ + GLCG F + +DF + + + + T SC + P + + C+ ++N P F+ C ++V+ + + C E ++ + C + A C G+ V +L C C G V+Q C+ +C+STC L + C ++C GC CP G CV +S+C C S P G+ + + C+ C C G +C + + C + +CP+N + S C +C N + + +S I +C GC T DG+ CVQP++CPC + + + IT+ C +C+ ++W E C VC YGDPHYKT DG+ + FQG+C Y L T GD N+ ++V N+ CG S TC K IG + + G+ I L P + +I + + V IG+ + WD STR+Y+ L P + G V+GLCGN++GD ++++ + + F +S+ +A P + D C +P R +W+++ CGV++ + +FA C + V Y CLYDACGC+ GGDCECLCT++A +A +C+ G+P++WR+ LC Sbjct: 1 QVTLDNGMRIVWDGVRRLYVTAPPELEGTVAGLCGTFTRDQRDDFMTRAGDVEIEVWAFTARWKVAQGATNSCPEANSLALASPCNTYAQLNSAAQETCNKLMNDP-FNLC--HTRVNPDAFKKDCTYEVCTAGALTGAECKTFADYAYICASRGV---VLANWRLLLDCTVTC-PGDMVYQQCASSCRSTCASL--SNIEDCHEECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSSTNYAP----GSILQMGCKNCTCEYGGWSCWSSEGCVDDSGLCPENMQWSPCKSSCPVTCGNMNNPSLSSCITETCDAGCECSTGLVWDGSKCVQPANCPCYHGGQSFQTGQSITLDCNNCVCNGQVWEC---EHKRCPGVCSVYGDPHYKTFDGRAYDFQGNCQYVLARTIYSLIDYSSLNLTDMGDVDND--GFVLTVENVPCGSSGVTCTKN----IGLKYWRAHNGDIFKIDLVRGKNVVDPTGNFIIRRAGLYL-FVDTPIGVSIQWDYSTRLYIRLHPKYMGLVEGLCGNFNGDQNDDFVTPAAGPPEEMPNIFGDSWKVHAYCAPAVVI----------NDTCLTHPHRAAWSQRKCGVLRSS--LFADCQGVVA--VAPYVDRCLYDACGCDSGGDCECLCTAIAAYAYECSANGVPIEWRSDELC 634 HSP 2 Score: 114.39 bits (285), Expect = 5.263e-26 Identity = 106/405 (26.17%), Postives = 156/405 (38.52%), Query Frame = 3 Query: 1593 CPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKV-GRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESM--LGTEGNSAYINEPKIVTVKVNG----SDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICST-------------ILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAG 2735 CP N WS C S CPVTC N+ + C C C +G V G C++P +CPC + GQ G DCN C CN +W C K CP C + G + TFDG + F CQYV ++ +I ++ ++ +G N ++ + V +G +I LK GD + G++ P N +R+ +Y V + +D S +YI++ + GLCG F+G+ +DF E + NI P ++ ND C T +L F+ C V Y C + S D C+AI A C G+ E + S+ L PLC+ G Sbjct: 267 CPENMQWSPCKSSCPVTCGNMNNPSLSSCITETCDAGCECSTGLVWDGSKCVQPANCPCYHGGQSFQTGQSITLDCNNCVCNGQVWECEHKRCPGVCSVYGDPHYKTFDGRAYDFQG-NCQYVLAR----------TIYSLIDYSSLNLTDMGDVDNDGFVLTVENVPCGSSGVTCTKNIGLKYWRAHNGDIFKIDLVRGKN------VVDPTGNFIIRR-AGLYLFVDTPIGVSIQWDYSTRLYIRLHPKYMGLVEGLCGNFNGDQNDDFVTPAAGPPEEMPNIFGDSWKVHAYCAPAVVI------NDTCLTHPHRAAWSQRKCGVLRSSLFADCQGVVAVAP--YVDRCLYDACGCDSGGDCECLCTAIAAYAYECSANGVPIE--WRSDELCRKSPLCKKG 643 HSP 3 Score: 100.908 bits (250), Expect = 8.025e-22 Identity = 76/263 (28.90%), Postives = 122/263 (46.39%), Query Frame = 3 Query: 3630 GLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDAC--GCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNG-MIDLNG-ECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 G+R++WD R+Y+ P +G V GLCG + D ++++M E + + A N++A + CN S A++ C K N F C ++ D + +C Y+ C G G +C+ + A +A CA G+ + WR C + C G Y CAS C TC ++ + H C EGC+CP+G ++D +G CV + C C Y+ +Y G+I++M CK C C ++C Sbjct: 7 GMRIVWDGVRRLYVTAPPELEGTVAGLCGTFTRDQRDDFMTRAGDVEIEVWAFTARWKVAQGATNSCPEANSLALASP---CNTYAQLNSAAQETCN--KLMNDPFNLCHTRVNPDA--FKKDCTYEVCTAGALTGAECK----TFADYAYICASRGVVLANWRLLLDCTVTCPGDMVYQQCASSCRSTCASLSNIEDCH---EECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSSTNYAPGSILQM-GCKNCTCEYGGWSC 254 HSP 4 Score: 62.7734 bits (151), Expect = 4.991e-10 Identity = 52/195 (26.67%), Postives = 79/195 (40.51%), Query Frame = 3 Query: 1296 CPDIANTLSTCKPDDQIL-----ANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVE--YGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHC 1853 CP+ AN+L+ P + A + C+K+ F C VN D + C +CT +++ C + Y+ C+ +G + WR C CPG+ ++ CAS C TC +L C C C G + G +C+ C C Y + PG++ Q C CTC W C Sbjct: 73 CPE-ANSLALASPCNTYAQLNSAAQETCNKLMNDPFNLCHTRVNPDAFKKDCTYEVCTAG----------ALTGAECKTFADYAYICASRGVVLANWRLLLDCTVTCPGDMVYQQCASSCRSTCASLSNIED--CHEECAEGCNCPDGLLLDFDGLSCVDQSQCKCSYSSTNYAPGSILQMGCKNCTCEYGGWSC 254 HSP 5 Score: 62.003 bits (149), Expect = 1.104e-9 Identity = 71/286 (24.83%), Postives = 121/286 (42.31%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTW-SINIVYENCTSDTGCVKVIV-IEAGDVVVKSSGN-GILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS------FGNSFK--GFGCPDIA-NTLSTCKPDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCE 1586 C YG +Y+TFD + Y +QG C Y+L A + S + + S+N+ + G V + + G V + N G+ Y N + + + + ++ T +++R+ L + ++WD + + Y G V GLCGN + + ++DF FG+S+K + P + N P + ++C +R F C +V Y C+ + C D + LCT ++AY+ CS G I+WRSD LC Sbjct: 362 VCSVYGDPHYKTFDGRAYDFQGNCQYVL-ARTIYSLIDYSSLNLTDMGDVDNDGFVLTVENVPCGSSGVTCTKNIGLKYWRAHNGDIFKIDLVRGKN--VVDPTGNFIIRRAGLYLFVDTPIGVSIQWD-YSTRLYIRLHPKYMGLVEGLCGNFNGDQNDDFVTPAAGPPEEMPNIFGDSWKVHAYCAPAVVINDTCLTHPHRAAWSQRKCGVLRSSLFADCQGVVAVAPYVDRCLYDAC--------GCDSGGDCECLCTAIAAYAYECSANGVPIEWRSDELCR 635 HSP 6 Score: 57.7658 bits (138), Expect = 1.939e-8 Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 3 Query: 4134 MCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC 4403 +C + C S CP TC + + + S C T C GC C G++ +CV P CPC++ GQS+ G I ++ C C C + C Sbjct: 266 LCPENMQWSPCKSSCPVTCGNMNNPSLSSCITETCDAGCECSTGLVWDGSKCVQPANCPCYHGGQSFQTGQSITLD-CNNCVCNGQVWEC 354
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009153.1 (pep scaffold:Pmarinus_7.0:GL479660:8329:29413:-1 gene:ENSPMAG00000008271.1 transcript:ENSPMAT00000009153.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 152.91 bits (385), Expect = 8.296e-39 Identity = 145/491 (29.53%), Postives = 206/491 (41.96%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGC-NSCTNY---DTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPC-----IY--MNREYAPNDVIT---------IKCAKYICSNRMWNLLSEETANCEAV----CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANI-ACGFSLATCAKFVQI-IIGGQTYLLSR-------GNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIANA---DICPYEISPENNVAPSKEQD-MCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGI-PVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENC-KKCQCV 4385 C N Y D S C N+C + D C T + C C + V D CV DC C IY + + ++ T + C + C L C+ V C GDPHY+T DG ++F G+ Y L+ T GD+ N I PI + N G S + V + + G + SR G P+FP+ + I+Q + L +D GL V++DR + + S+ V GLCGNY+GD +EY + +I+ F ES+ N ++ P + +++ D C+ + CG++KD G F C E + D YY NCLYD C GD E LC S+A + C GI P WR + C C Y + CP +C + AP C +EGC C G + CVP C CF++ Y G C +CQCV Sbjct: 71 CLANSHYTDCASLCPNTCLHASTEDDCRSTETCTEGCVCDSGFVLS-DRLCVPQQDCGCWDNGKIYHTLGESWLTDNCSTQCRCMGPEEMNCTLHSCMAEESCALQNGQYKCQPVGFSTCTISGDPHYQTFDGLPYNFMGNNTYVLVQTSGDNPNLI-PINVRGKNANRHGLSDVSYLDEVHVNVFGHEIRFTSRCQLPYFDGVRMRPPMFPREGLSIQQSSHRIILQTD-FGLTVIYDRREHADVIISSSYMNEVSGLCGNYNGDPNDEYFGRNGQQIPSIKVFGESWAMNGASENLAPAVSQYKFSISEDSLNDPTCSGTQLIELQGISSCGMIKDMAGPFQPCQETVSPD--SYYENCLYDMCA--NFGDKETLCNSVAVYTAACQRKGITPSNWRNFSGCDAACPPNSIYTESMTACPASCGNLA--APEECGLPR-MEGCQCELGFVWSGFHCVPFRDCGCFHDDTYYEVGKEFYSPQCSSRCQCV 551 HSP 2 Score: 88.1965 bits (217), Expect = 5.676e-18 Identity = 131/559 (23.43%), Postives = 224/559 (40.07%), Query Frame = 3 Query: 1383 FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQ-EHPPGTVRQFD-CNT-CTC------NSSLWHC-TEKICP-------------STCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKL--YFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDN------------TPQSIVDPPAGDNDI--------CSTI--LNGPEFSGCMNSSKVDKEFYATYCRREQSSN-KDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWN 2909 F C ++N Y + C ++ S ++ +LC L AY+++C +G IQWR++T C C N ++DCAS CP TC + + C+C SG V C+ + C C +G+ H G D C+T C C N +L C E+ C STC I G + TFDG + F YV V+ S D N+ I + + +Y++E V V V G +I + QL + DG P P + + +++ ++ + + + F L +D + + +S + N + GLCG ++G+ +++ ++ SI +S+ + G+ +N + S+ DP + C I + GP F C + V + Y C + +N D+ + C+++ AC+R GI P C C +++ C ++C L A +C GC+C + +G CV +C C++ Sbjct: 11 FASCHHVINPQDYVTTCTFDMGQYS----------GMASILCDSLQAYAEACRVEGVNIQWRNETFCPLSCLANSHYTDCASLCPNTCLHASTEDDCRSTETCTEGCVCDSGFVLSDRLCVPQQDCGCWDNGKIYHTLGESWLTDNCSTQCRCMGPEEMNCTLHSCMAEESCALQNGQYKCQPVGFSTCTISGDPHYQTFDGLPYNFMGNN-TYVLVQTS----GDNPNLIPINVRGKNANRHGLSDVSYLDE---VHVNVFGHEIRFTSRC-QL------PYFDG---VRMRPPMFPREGLSIQQSSH---RIILQTDFGLTVIYDRREHADVIISSSYMNEVSGLCGNYNGDPNDEYFGRNGQQIPSIKVFGESWAMNGASENLAPAVSQYKFSISEDSLNDPTCSGTQLIELQGISSCGMIKDMAGP-FQPCQET--VSPDSYYENCLYDMCANFGDKETLCNSVAVYTAACQRKGI---TPSNWRNFSGCDAACPP-NSIYTESMTACPASCGNL--AAPEECGLPRMEGCQCELGFVWSGF-HCVPFRDCGCFH 528 HSP 3 Score: 81.6481 bits (200), Expect = 7.730e-16 Identity = 59/169 (34.91%), Postives = 83/169 (49.11%), Query Frame = 3 Query: 3915 CGVMKDTNGVFASCIEAMH-QDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHC-STTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVN-GAIIKMENCK-KCQCV-QAKFNCT 4406 C V+ G FASC ++ QD Y + C +D G LC SL +A C G+ ++WR + CP+ C HY CAS CP TC + ++ C ST C EGC C +G + + CVP C C+ NG+ Y G +NC +C+C+ + NCT Sbjct: 1 CQVLTSPTGPFASCHHVINPQD---YVTTCTFDMG--QYSGMASILCDSLQAYAEACRVEGVNIQWRNETFCPLSCLANSHYTDCASLCPNTC--LHASTEDDCRSTETCTEGCVCDSGFVLSDRLCVPQQDCGCWDNGKIYHTLGESWLTDNCSTQCRCMGPEEMNCT 162 HSP 4 Score: 63.5438 bits (153), Expect = 3.153e-10 Identity = 81/357 (22.69%), Postives = 148/357 (41.46%), Query Frame = 3 Query: 783 GLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXX-----XXXXXXXXXXXXVASFGNSFKGFGCPD-IANTLSTCK--PDDQILANQQCSKIRL-----------------KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKG-SCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYD 1775 G +Y+TFD Y + G +Y+L S + IN+ +N + G V ++ +V V G+ I +T+ P + P + + + + I+L + G + + +D + + I +S +V+GLCGN + + ++ FG S+ G + +A +S K + L + CS +L F C + V+ D+Y+ C+ ++C + T LC ++ Y+ +C KG + WR+ + C+ CP N I+++ + CP +C NL C ++ C C G V G+ C+ C C +D Sbjct: 193 GDPHYQTFDGLPYNFMGNNTYVLVQTSGDNPNLIPINVRGKN-ANRHGLSDVSYLD--EVHVNVFGHEIRFTSRCQLPYFDGVRMRPP---MFPREGLSIQQSSHRIILQTDFG-LTVIYDRREHADVIISSSYM-NEVSGLCGNYNGDPNDEYFGRNGQQIPSIKVFGESWAMNGASENLAPAVSQYKFSISEDSLNDPTCSGTQLIELQGISSCGMIKDMAGPFQPCQETVSPDSYYENCLYDMCANFGDKET----------LCNSVAVYTAACQRKGITPSNWRNFSGCDAACPPNSIYTESMTACPASCGNL--AAPEECGLPRMEGCQCELGFVWSGFHCVPFRDCGCFHD 529
BLAST of SCO-spondin vs. Ensembl Nematostella
Match: EDO44199 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWN6]) HSP 1 Score: 255.758 bits (652), Expect = 1.304e-68 Identity = 307/1209 (25.39%), Postives = 483/1209 (39.95%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSY-ILTAPSDVSAVTWSINIVYENCTSDTGCVKVI-------VIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPD-----------DQILANQQCSKI-----RLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCS-DKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEP--SVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIIL----KATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY------ITGSCDNTPQSIVDPPAGD--------NDICSTI--LNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIP--LCQAGGTVFQICSR-------TCQSTCRELNEATASKCDDDCFPGCECPPN-YYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGS-CMYTLMDTPGDSSNNIPPITISVANIAC--GFSLATCAKF--------VQIIIGGQTYLLSRGNNDTI-----------PLFPKYDIVIE--QLTFFT------QLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAI-QFAESF-IANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLC 4127 C+ G ++ TFD+Q Y++ G CSY L S+V + + +NC + C K + + G V G + N P T S N A G + V D ++ + + +K ++ S + GLCGN D N +NDF D ++ FG + + L T P D A Q K+ F C V+ + + C+ ++C N+T S SD +C LS YS +C+ + + WRS +LC CP S+C+S CP C N+ P+A C + V C C G+++ C+ P C C + G + G +R+ +C++C CN +W+CT+ C TC ++G + F TFD ++ + C YV E N+T + + + I S+ L A + V GS +L KATS L V +N+ V + + + G + YI V K+ GLCG F+ N +DF +D A+ + +C + + +D P C+ I LNGP F C V + C ++ KD L CS A AC GI + + + C P +C AG TV + C+R C STC +L T + C GC C +CV +++C C+ D G C C C G +C D C + VY D C + + + C CP+ + +GTCVQ C C + N+ Y + C++ +R W L+ A E C A+G HY+T DG +++F+ C YT M+ + + V N+ C F L C + V++++ +++ L R DT P Y +E Q+ F ++ + I + +D+ TRVY+ ++G VQGLCGN+DG N+ T +I +FA+S+ + +A + +SP C PDRK WA++ C ++ N F C +A++ Y C Y+ C + GGDC C+++A + C GI + WR + C Sbjct: 478 CYISGRGHFTTFDHQEYSFYGTCSYKALIDNSNVKQIFQVVLNNDKNCDINAPCTKSVDVNVDGKKVHLGQEVASRHGFVKVDGVTENLPYTK-SWPNIRQA----GKYVIVRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTR-----LKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLDDPLCTNPPKPPSGCYSEVAGDVFSAMQNAEKMCSVIQSDAFSACRSKVDFRDFKAQCIADVCA----CNST----SRSDCMCNVLSTYSKACAWNHNITLSWRSPSLCPVTCPSGMEHSECSSACPRDCSNMNSDPNA-CNSKCVDGCFCPEGKIQDRGKCVDPGQCSCYHSGIPYAHGAIRKSECSSCLCNGGMWNCTDTPCSGTCTMVGNSHFTTFDSRSYSMHG-QCDYVLSEHCHFSNDTTKQFTIHIKISSDGSRQLIVSLAGA----TPVTLGGVEGSPTVLNSNGKATSHILSVEHIGV-----------------QNVLVHAYAGL----------TILWTGYNA-YITVGPDFKSQTCGLCGTFNQNDADDFHTRDGDNEADAYAFAQEWQVVSSVAASNCHRSNWNQLDQPCSTYSSNEGYAKSGCNAIIDLNGP-FKACHR--YVPPQDMYQKCVKDGCMCKDCL--CSVAAAYAKACADKGIV--IDGWRDTISVCKPKFVCPAG-TVLKQCNRDPLTRKLRCPSTCDDLANVTDTCPSRRCVEGCYCEKEGELMNNEHKCVDKTQCPCYY----GDTMYKYGEIRKDRCNNCTCKGGVFDCTNVD-CEAMCLTRGLVYSDCGITCENL--HPINGGERPCVSGCYCPDGLIMHDNGTCVQSMQCQCKHNNKYYDAGAISPTDCSRKCRGDRYWEQLAGIQAQPEYECSAFGKGHYRTFDGMLYNFESEGCGYTFMEHED--------VKVVVKNMKCVNSFELQMCKEVTITLVKENVEVVMMQKSFTLKRNGIDTAYNPGSYPPPCKPFLNTYMNTLEIFQVGLFMIVRIVDRVNPNHIKFEIRFDQGTRVYVMANTDYKGTVQGLCGNFDGKDSNDKRTSTGSIATSIPEFADSWRVGSACLDTKTVSP------------CVLYPDRKPWADKGCEEIR-ANPNFTICHDAVNP--APYIEACKYETCMSHMGGDCGAFCSAIAAYVRACNRYGISILWRREGFC 1596 HSP 2 Score: 148.673 bits (374), Expect = 1.127e-35 Identity = 121/420 (28.81%), Postives = 196/420 (46.67%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQ-----------LVSDSIGLRVLWDRSTRVYLFLKPSFQG------NVQGLCGNYDGDLQNEY--MHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAF-LGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTP---SQFRCDDGKCIESSKICDKV 4502 +C G H+ T D Q +SF G+C Y + D+SN + + N A C K V + + G+ L + + F K D V E L + +V S + + +D S + + + S++ ++GLCGNYDG+ N++ + GT +D I + + +C P + D+ +A + AE+MC V++ + F++C + D + + C+ D C CN +C+C L+T++ CA+ I + WR+ +LCP+ C G + C+S CP+ C + S+ P+ C++ CV+GC CP G I G+CV P C C+++G Y +GAI K E C C C +NCT+ + C TCT S F D + CD V Sbjct: 476 GLCYISGRGHFTTFDHQEYSFYGTCSYKALI---DNSNVKQIFQVVLNNDKNCDINAPCTKSVDVNVDGKKVHLGQ-EVASRHGFVKVDGVTENLPYTKSWPNIRQAGKYVIVRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTRLKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLDDPLCTNPPKPPSGCYSEVAGDVFSAMQN----AEKMCSVIQ--SDAFSACRSKV--DFRDFKAQCIADVCACNSTSRSDCMCNVLSTYSKACAWNHNITLSWRSPSLCPVTCPSGMEHSECSSACPRDCSNMNSD-PNACNS-KCVDGCFCPEGKIQDRGKCVDPGQCSCYHSGIPYAHGAIRKSE-CSSCLCNGGMWNCTD--TPCSGTCTMVGNSHFTTFDSRSYSMHGQCDYV 878 HSP 3 Score: 123.635 bits (309), Expect = 4.267e-28 Identity = 134/505 (26.53%), Postives = 205/505 (40.59%), Query Frame = 3 Query: 3030 VCPKNQVYMDSVSGC-NSCTNYDTCTDT--SSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS--LATCAKFVQIIIGG----QTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMH-QDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WR-TQNLCP--IMCEGGKHYDACAS-------PCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGE--CVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE------------DYSDCITTC 4436 CP + + S C C+N ++ + S + C CP + G CV P C C + YA + +C+ +C+ MWN C C G+ H+ T D + +S G C Y L + S++ TI + + G + + A + +GG T L S G + + + +E + LV GL +LW Y+ + P F+ GLCG ++ + +++ E DA FA+ + + + N ++ C+ + +A+ C + D NG F +C + QD+ Y C+ D C C +CLC+ A +A CA GI + WR T ++C +C G C CP TCD + +N C + CVEGC C +N E CV T CPC+Y Y G I K + C C C F+CT YSDC TC Sbjct: 758 TCPSGMEHSECSSACPRDCSNMNSDPNACNSKCVDGCFCPEGKIQD-RGKCVDPGQCSCYHSGIPYAHGAIRKSECSSCLCNGGMWNCTDTP---CSGTCTMVGNSHFTTFDSRSYSMHGQCDYVLSEHCHFSNDTTKQFTIHIKISSDGSRQLIVSLAGATPVTLGGVEGSPTVLNSNGKATS------HILSVEHIGVQNVLVHAYAGLTILW-TGYNAYITVGPDFKSQTCGLCGTFNQNDADDFHTRDGDNEADAYAFAQEWQVVSSVAASNCHRSN---WNQLDQPCSTYSSNEGYAKSGCNAIIDLNGPFKACHRYVPPQDM---YQKCVKDGCMCK-----DCLCSVAAAYAKACADKGIVIDGWRDTISVCKPKFVCPAGTVLKQCNRDPLTRKLRCPSTCDDL-ANVTDTCPSRRCVEGCYCEKEGELMNNEHKCVDKTQCPCYYGDTMYKYGEIRK-DRCNNCTCKGGVFDCTNVDCEAMCLTRGLVYSDCGITC 1238 HSP 4 Score: 87.0409 bits (214), Expect = 5.378e-17 Identity = 111/409 (27.14%), Postives = 174/409 (42.54%), Query Frame = 3 Query: 1869 PSTCGIMGT-QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEP--KIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKN------NLIGLCGKFDGNSENDFTEKDNS--IRESIVNIAKSYITGS-CDNTPQSIVDPPAG-----DNDICSTILNGPE---------FSGCMNSSKVD-KEFYAT----YCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNC 3002 P C I G F TFD +++F C Y A+ ++N++ + +F ++ +N ++ IN P K V V V+G + L ++ V +DG T LP+T NI + Y V+ S +YFDGS+ + IKV D+ K L GLCG +DGN+ NDF D + +++I + S + C N P+ PP+G D+ S + N + FS C SKVD ++F A C +S D + C+ + + AC + + + C P+ G CS C C +N + + C+ C GC CP + G+CV +C+C++ P G C +C+C G NC Sbjct: 475 PGLCYISGRGHFTTFDHQEYSFYGT-CSYKAL---IDNSNVK-QIFQVVLNNDKN--------CDINAPCTKSVDVNVDGKKVHL---GQEVASRHGFVKVDGV--TENLPYTKSWPNI---RQAGKYVIVRTSDEVLIYFDGSEELMIKVPDSYKTVNSSDTRLKGLCGNYDGNNTNDFIGLDGTTYTKDNINDFGVSMLDDPLCTNPPK----PPSGCYSEVAGDVFSAMQNAEKMCSVIQSDAFSAC--RSKVDFRDFKAQCIADVCACNSTSRSDCM--CNVLSTYSKAC---AWNHNITLSWRSPSLC-PVTCPSGMEHSECSSACPRDCSNMN-SDPNACNSKCVDGCFCPEGKIQD-RGKCVDPGQCSCYHSGIP----YAHGAIRKSECSSCLCNGGMWNC 844
BLAST of SCO-spondin vs. Ensembl Nematostella
Match: EDO41877 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S313]) HSP 1 Score: 183.726 bits (465), Expect = 5.978e-51 Identity = 113/342 (33.04%), Postives = 165/342 (48.25%), Query Frame = 3 Query: 3123 NSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN--------NDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQ--GNVQGLCGNYDGDLQNEYMHGTMKYEDA-IQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWR 4112 + C CP +V G C+ ++CPC + + Y +DVI C C W + C A C A+GDPHYKT DG+++SFQG C Y + D S + I NI CG + TC K V + + + RG + P+ +Y++V L ++ GL V+WDR TR+Y+ L P Q V GLCGN+DGD+ N+YM E + F +++ A D C + P + P C N R+S + C ++K +G F C ++ D C+YD CGC+ GDCEC C ++A +A +C G+ VKWR Sbjct: 12 SGCVCPVGSVLDDFGRCILETECPCHHNGKSYKTDDVIRRDCNTCTCVRNTWECTKKR---CRATCTAFGDPHYKTFDGRLYSFQGGCNYVMSTDKCPGSKLTLQSFRIDAENIPCGSAGVTCTKSVTVTLHDTIIHMVRGKAVPSVTKVSSLSPVHARYELVYAGLYV---IIKAPFGLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTWRARGD-CTKTMEP---IHP------CKQNKHRESKSHAACAIIK--SGTFKPCHISVDPDPYYERCRCVYDTCGCDSVGDCECTCEAIAAYAYECLAAGVVVKWR 335 HSP 2 Score: 83.1889 bits (204), Expect = 9.031e-17 Identity = 73/263 (27.76%), Postives = 113/263 (42.97%), Query Frame = 3 Query: 1701 CICGSGEV--EYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKN--NLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSI 2471 C+C G V ++G CI CPC ++G+ + V + DCNTCTC + W CT+K C +TC G + TFDG ++F GC YV +TD + +QS SA + K VTV ++ D I+ + S TK+ P + +Y +K + +D +YI++S ++ + GLCG FDG+ ND+ K+ S ++ G C T + I Sbjct: 14 CVCPVGSVLDDFG-RCILETECPCHHNGKSYKTDDVIRRDCNTCTCVRNTWECTKKRCRATCTAFGDPHYKTFDGRLYSFQG-GCNYVM------STDKCPGSKLTLQSFRIDAENIPCGSAGVTCTKSVTVTLH--DTIIHMVRGKAVPS-----------VTKVSSLSPVHARYELVYAGLYVIIKAPFGLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTWRARGDCTKTMEPI 255 HSP 3 Score: 50.8322 bits (120), Expect = 2.712e-6 Identity = 31/87 (35.63%), Postives = 45/87 (51.72%), Query Frame = 3 Query: 4221 CSTTHCVEGCSCPNG-MIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTP---SQFRCDDGK 4469 C C GC CP G ++D G C+ T CPC +NG+SY +I+ +C C CV+ + CT+ C TCT ++ DG+ Sbjct: 5 CGQVFCKSGCVCPVGSVLDDFGRCILETECPCHHNGKSYKTDDVIR-RDCNTCTCVRNTWECTK--KRCRATCTAFGDPHYKTFDGR 88 HSP 4 Score: 50.447 bits (119), Expect = 3.854e-6 Identity = 64/286 (22.38%), Postives = 126/286 (44.06%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAP----SDVSAVTWSINIVYENC-TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXX-XXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG-CPDIANTLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCT--HLSAYSDSCSDKGSCIQWRSD 1574 TC ++G +Y+TFD +LY++QG C+Y+++ S ++ ++ I+ C ++ C K + + D ++ + + + L+P+ + L+ G +YV+ + F + + WD ++I + +S + V GLCGN D + +ND+ + + FG++++ G C + CK + + + +K F C V+ D Y+ C + T S+ D CT ++AY+ C G ++WR D Sbjct: 73 TCTAFGDPHYKTFDGRLYSFQGGCNYVMSTDKCPGSKLTLQSFRIDAENIPCGSAGVTCTKSVTVTLHDTIIHMVRGKAVPSVTKVSSLSPV---HARYELVYAG--LYVIIKAPF--------GLNVIWDRGTRLYIELSPDSQHWSKVCGLCGNFDGDMTNDYMAKNGITETSPYPFGDTWRARGDCTKTMEPIHPCKQNKHRESKSHAACAIIKSGTFKPCHISVDPDPYYERC--------RCVYDTCGCDSVGDCECTCEAIAAYAYECLAAGVVVKWRQD 337
BLAST of SCO-spondin vs. Ensembl Nematostella
Match: EDO41876 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S312]) HSP 1 Score: 137.117 bits (344), Expect = 1.473e-34 Identity = 105/389 (26.99%), Postives = 175/389 (44.99%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMD--TPGDSSNNIPPITISVAN-IACGFSLATCAKFVQIIIGGQTY-------LLS-----RGNNDTIPL-FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMK--YEDAIQFAESFIANA--DIC--PYEISPENNVAPSKEQDMC-NANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 C +G HY+T DG+ F G C YTL PG ++ V N CG + C + + + IG +TY L+S R N + + ++ + ++ +T + S L + +D ++ + + + ++ N GLCGN +G ++ + +FAES+ + C P E++ +D+C NA+ + A+++C V+ N F++C + D + C D C C G C C +L ++ CA I + WR+Q+LCP C K Y C C +TCD G H C++GC CP G + + C+P CPC +NG +YV G I++ C C+C + C++ Sbjct: 2 GACSTWGQRHYRTFDGRHIDFIGKCSYTLAQDCIPGSDQ-----FSLHVQNDKTCGSAGMDCERALLLYIGEKTYKVRWLVHLISSHGAIRVNGAKVSAPYKDTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYRNNTCGLCGNNNGIPDDDLLMAVTNKPATSVAEFAESWAMPEPNEKCESPLEVT----------RDVCSNASSVIRDAAKRICMVLLSEN--FSACHTKV--DPVPFVKRCEEDVCSCKFGEHSGCQCQALTEYSRACASREIMLDWRSQHLCPKQCIQPKVYSECGPACVKTCDADGLQPTCH---ETCIDGCHCPEGTVQTDNRCLPVNQCPCQHNGITYVTGTTIRV-GCNTCKCRGGSWTCSK 367 HSP 2 Score: 82.8037 bits (203), Expect = 1.996e-16 Identity = 83/383 (21.67%), Postives = 157/383 (40.99%), Query Frame = 3 Query: 783 GLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTG--CVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXX------XXXXXXXXXXXXVASFGNSFKGFGCPD--------IANTLSTCKPDDQIL--ANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICP 1871 G +YRTFD + + G+CSY L + +S+++ + G C + +++ G+ K +++ S+ + V + T + V + ++ ++ + + +K+D ++I +++ + + GLCGN + + +VA F S+ P+ + T C ++ A ++ + L F C V+ + C +++C+ S C L+ YS +C+ + + WRS LC QC K++S+C C TC P+ C + + C C G V+ C+ CPC ++G + GT + CNTC C W C++ CP Sbjct: 8 GQRHYRTFDGRHIDFIGKCSYTLAQDCIPGSDQFSLHVQNDKTCGSAGMDCERALLLYIGEKTYKV--RWLVHLISSHGAI---RVNGAKVSAPYKDTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYR-NNTCGLCGNNNGIPDDDLLMAVTNKPATSVAEFAESW---AMPEPNEKCESPLEVTRDVCSNASSVIRDAAKRICMVLLSENFSACHTKVDPVPFVKRCEEDVCS--------CKFGEHSGCQCQALTEYSRACASREIMLDWRSQHLCPKQCIQPKVYSECGPACVKTCDADGLQPT--CHETCIDGCHCPEGTVQTDNRCLPVNQCPCQHNGITYVTGTTIRVGCNTCKCRGGSWTCSKHRCP 371 HSP 3 Score: 58.151 bits (139), Expect = 1.869e-8 Identity = 74/315 (23.49%), Postives = 114/315 (36.19%), Query Frame = 3 Query: 2130 QLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEK-DNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICS--------------TILNGPEFSGCMNSSKVDKEFYATYCRREQSS---NKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNC 3020 L S + ++G K+ D N+ V K+ + + FDG+ SI + VS +NN GLCG +G ++D N S+ A+S+ + +S P D+CS +L FS C +KVD + C + S + C A+ + AC I + + CI V+ C C TC + C + C GC CP +T +C+ ++C C + GT I + C TC C G C +K C Sbjct: 78 HLISSHGAIRVNG----AKVSAPYKDTNVEVSKVHSYTIIRSQGDELGIKFDGASSIQVAVSHKYRNNTCGLCGNNNGIPDDDLLMAVTNKPATSVAEFAESWAMPEPNEKCES---PLEVTRDVCSNASSVIRDAAKRICMVLLSENFSAC--HTKVDPVPFVKRCEEDVCSCKFGEHSGCQCQALTEYSRACASREIMLDWRSQHLCPKQCIQ-----PKVYSECGPACVKTCDA--DGLQPTCHETCIDGCHCPEGTVQTDN-RCLPVNQCPCQH----NGITYVTGTTIRVGCNTCKCRGGSWTC-SKHRC 370
BLAST of SCO-spondin vs. Ensembl Nematostella
Match: EDO33061 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST99]) HSP 1 Score: 100.908 bits (250), Expect = 8.492e-23 Identity = 70/281 (24.91%), Postives = 131/281 (46.62%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPL----FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDA-IQFAESFIANADICPYEISPENNVAPS----KEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLC 4127 +C +YGDPHY+T DG+MF+F G+C Y L+ + ++ + N A G + + +I Q L+ + + + L P + I++ F L+S++ G++++WD + + + + ++GN GLCGN++G +++M +Y + +F +S+ + + P APS + C + A + C +K F++C + ++YY C+ + C C C C SL T+A C +G+ V W + C Sbjct: 55 GICTSYGDPHYQTFDGRMFNFHGNCRYRLVADCRQGNFSVRLRSERHHNQAVGEAKSLVVHIGSTVIHLQRGLVVKVDRKEVQLPYQRLP--SLQIDRKNFMITLLSET-GVKIIWDGESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSW----SLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWPIKSQ---FSNCHPVV--KPHKYYKACISEVCKCLVK---RCECESLVTYARACRRMGVNVNWGRKKAC 320 HSP 2 Score: 66.6254 bits (161), Expect = 2.316e-11 Identity = 65/297 (21.89%), Postives = 108/297 (36.36%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSD-TGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXX-----XXXXXXXXXXVASFGNSFKGFG-----------CPDIANTLSTCKPD--DQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCET 1589 E+ C SYG +Y+TFD +++ + G C Y L A D +S+ + E + G K +V+ G V+ G++ D P P + + NF++ L +K+ WD + + Y G+ GLCGN + + + FG S+ G P IA +S C+ A ++C I+ +F C +V Y+ C+ +C + C L Y+ +C G + W C T Sbjct: 52 EDMGICTSYGDPHYQTFDGRMFNFHGNCRYRLVA--DCRQGNFSVRLRSERHHNQAVGEAKSLVVHIGSTVIHLQ-RGLVVKVDRKEVQLPYQR-----------LPSLQIDRKNFMITLLSETGVKIIWD-GESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSWSLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWPIKSQFSNCHPVVKPHKYYKACISEVCK-----------CLVKRCECESLVTYARACRRMGVNVNWGRKKACGT 322 HSP 3 Score: 57.7658 bits (138), Expect = 1.759e-8 Identity = 64/277 (23.10%), Postives = 101/277 (36.46%), Query Frame = 3 Query: 1896 QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPF-TIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTP--QSIVDPPAGD---------------NDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGI 2672 + TFDG F F+ C+Y V D R F + + ++ + E K + V + + I L Q + + + +LP+ +P I + N + + K+ +DG I + VS + N GLCG F+G +DF K+ S KS+ G P Q PP + C I + +FS C K K + A C E + C +++ A ACRR G+ Sbjct: 64 HYQTFDGRMFNFHG-NCRYRLV------ADCRQGNFSV------RLRSERHHNQAVGEAKSLVVHIGSTVIHL----------QRGLVVKVDRKEVQLPYQRLPSLQIDRK---NFMITLLSETGVKIIWDGESYIEVHVSHRYRGNTCGLCGNFNGVPSDDFMMKNGRYARSDREFGKSWSLGGHRRRPCLQRAPSPPIAEPISKCRRSALHHWRARKRCWPIKS--QFSNCHPVVKPHKYYKA--CISEVCKCLVKRCECESLVTYARACRRMGV 310
BLAST of SCO-spondin vs. Ensembl Nematostella
Match: EDO49498 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGB0]) HSP 1 Score: 95.1301 bits (235), Expect = 9.552e-20 Identity = 59/137 (43.07%), Postives = 77/137 (56.20%), Query Frame = 3 Query: 4431 TCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--KKCQMTEYKCNNSQICI 4820 +CTP+QF C G+CI CD DCS+ DE+ C C QF C SNG CI + +CDG +C D SDE +C C P + C R CI+ K ICDG DCG D+SDE +C + C ++C+N+ CI Sbjct: 124 SCTPAQFSCPSGRCIPLRWRCDGDGDCSDGADERGCPPKNCTDSQFSC-SNGQCISLAWRCDGDHDCADKSDERNCTGKTCKPFEFACANGRHCIQRKWICDGENDCG--DRSDEVDCGLESCGNDRWRCSNTSRCI 257 HSP 2 Score: 86.6557 bits (213), Expect = 4.422e-17 Identity = 63/168 (37.50%), Postives = 84/168 (50.00%), Query Frame = 3 Query: 4377 QCVQAKFNCTEDYSDCI-------------TTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNC--NVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISC---GCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--KKCQMTEYKCNNSQICI 4820 +C+ + C DY DC+ T C+ S FRC +G+C +CD DC + DEK C + C QF C S G CIP+ +CDG +C D +DE C C Q C+ + CI CDG++DC DKSDE NC K C+ E+ C N + CI Sbjct: 55 KCIPLSWRCDGDY-DCLAPDLSDEANCSVATNCSASMFRCANGQCKPRDWVCDGFDDCGDGSDEKGCANHSCTPAQFSCPS-GRCIPLRWRCDGDGDCSDGADERGCPPKNCTDSQFSCSNGQ-CISLAWRCDGDHDCA--DKSDERNCTGKTCKPFEFACANGRHCI 217 HSP 3 Score: 65.855 bits (159), Expect = 1.199e-10 Identity = 72/245 (29.39%), Postives = 98/245 (40.00%), Query Frame = 3 Query: 4365 CKKCQCVQAKFNC-----------TEDYSDCI-TTCTPSQFRCDDG-KCIESSKICDKVTDC--SNSEDEKNCNV---CDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCG---CLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENC--KKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNC 5030 CK C+ ++ C ++Y+ C +C S+FRC KCI S CD DC + DE NC+V C F+C +NG C P CDG +C D SDE C C P Q C R CI + CDG+ DC +D +DE C K C +++ C+N QCI+ +CDG +DC + DE+NC Sbjct: 8 CKNGHCISLRWKCDGENDCVDNSDEDEYAGCAEWSCRASEFRCQASRKCIPLSWRCDGDYDCLAPDLSDEANCSVATNCSASMFRC-ANGQCKPRDWVCDGFDDCGDGSDEKGCANHSCTPAQFSCPSGR-CIPLRWRCDGDGDC--SDGADERGCPPKNCTDSQFSCSNG--------------------------------------------------QCISLAWRCDGDHDCADKSDERNC 198
BLAST of SCO-spondin vs. Ensembl Medaka
Match: scospondin (SCO-spondin [Source:NCBI gene;Acc:101172449]) HSP 1 Score: 380.563 bits (976), Expect = 1.469e-106 Identity = 280/937 (29.88%), Postives = 428/937 (45.68%), Query Frame = 3 Query: 2151 NVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICST-------------ILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS-YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCREL------NEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTN-PVCPKNQVY--MDSVSGCNSCTNYDTCTDTSSIA--------NSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFP--------KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPE-NNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNC-------------TEDYSDCITTCTPSQFRCD--------------------DGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDC 4736 V ++G T LP D +H + ++ + V+ L+ I + L + + GLCG + +DFT + + S+ A+ +I+ Q P A +D CST +++ P F C + V+KE Y C E + Q S +C+ + A C + G+Q Q P+ +GG VF+ C RTC +C + N+++ C PGC+CP + GQCV S C C + P GT + NC TCVC +G NC T+++C + CP + VY + C+S ++ +SS+ + C CP T D CV P +CPC + + Y N+ IT C +C R W+ C+ VC+A GDPHY T DG+ +SF G C Y L ++ +I+ N+ CG + TC K V +G L RG T+ P + +E++ F L S +G+ ++WD RVY+ L +G V GLCGN+D D +N++ ++ +++ N+ +++SP +V QD C NP R +WA++ C V+ T +F+ C H +V YY C++D CGC+ GGDCECLCT++A++A +C G+ V+WR+Q LCP+ CE G Y+ C C Q+C ++ ++ S C CVEGC CP G I C+ P CPC + G + G I + C+ C CV+ + C +++ C PSQ+ CD +GKC+ SS +CD DC ++ VC F C CI +CDG +C D SDE C C P + C + C+ ++CDG+ DC Sbjct: 464 TVTVNGVRQT--LPVVSSDLAVH--RASSSFILVQTFGALVLWHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPEGDVEMSVSAFAEKFIS-------QPCTLPKAAPSDPCSTYTQRISYAERVCSVIHNPVFQKCHDV--VEKEPYFRLCLSEVCACALQRSCHCTVLTAYAQHCAQEGVQVHW-----RNQTFCPVQCSGGQVFRECGRTCGFSCFDWQHGWLCNDSSGEIGQRVCVPGCQCPTGLVQDHLGQCVPVSLCPCVQDDKIYSP----GTILQNNCNTCVCKHGAFNC-TQEHCKSVQQCPGSLVYSPRSCMLTCSS-LDHQGQQQSSSVTKPSCREPLSGCVCPQGTFFHGD-RCVLPDECPCHHNGQVYYTNETITKDCNTCVCQKRRWHCTR---LVCDGVCVATGDPHYVTFDGRRYSFLGDCQYVL------ATETSGLFSITAENVPCGSTGVTCTKSVTFSLGSTIIHLLRGKAVTVNGMPVTLPKSYSGSGLTLEKVGLFVSL-SSRLGVTLMWDGGMRVYVHLAAHLRGQVGGLCGNFDEDTENDFT----THQGSVESTAEVFGNS----WKVSPSCPDVENQDLQDPC-LNPHRVTWAKKRCAVL--TQDLFSVC----HTEVPFQHYYDWCVFDTCGCDSGGDCECLCTAIASYAEECNRRGVYVRWRSQELCPLQCEKGMIYEPCGPACSQSCPSVQQHSYSRCEVLSCVEGCFCPAGTIHHGDGCILPLDCPCEWEGSMFPPGTSI-TQRCQNCSCVEGLWQCEGVACPASSPPCLEAEFACATGRCIPSQWLCDNEDDCGDGSDEICPSTCSCANGKCLASSHVCDGRPDCGFADGSDEQGVC----FLCPGR-RCILYLHRCDGHDDCGDFSDERGCMCAPGEFQCPVG-NCVPAHRVCDGHRDC 1343 HSP 2 Score: 161.384 bits (407), Expect = 5.003e-39 Identity = 134/485 (27.63%), Postives = 198/485 (40.82%), Query Frame = 3 Query: 3030 VCPKNQVYMDSVSGCN-SCTNYDTCTDTSSIAN-------SCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLF-LKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTC-----DTIGSNAPSHCSTTHCVEGCSCPNGMI-DLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTC 4436 VCP+ QV+ D VS C SC + +++A C CP GTC++ DCPC + Y P D I +C +C + W E C C G T D + +S QG GD + T++V G T+P+ D+ + + + LV + G VLW L L+P V GLCG +++ E ++ FAE FI+ P APS D C+ R S+AE++C V+ N VF C + + ++ Y+ CL + C C C CT L +A CA G+ V WR Q CP+ C GG+ + C C +C + +++ CV GC CP G++ D G+CVP + CPC + + Y G I++ NC C C FNCT+++ + C Sbjct: 333 VCPRGQVFSDCVSSCPPSCASPHPPGPAATMAQCREECVGGCECPPGLFLH-QGTCLKAEDCPCFHRRLTYQPGDSIQQRCNTCVCRSGQWQCTGER---CAGQCTLVGALQVTTFDKKKYSLQG----------GDFLFQLLKCTVTV----------------------------NGVRQTLPVV-SSDLAVHRASSSFILV-QTFGALVLWHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPEGDVEMSVSAFAEKFISQPCTLP-------KAAPS---DPCSTYTQRISYAERVCSVIH--NPVFQKCHDVVEKEP--YFRLCLSEVCACALQRSCH--CTVLTAYAQHCAQEGVQVHWRNQTFCPVQCSGGQVFRECGRTCGFSCFDWQHGWLCNDSSGEIGQRVCVPGCQCPTGLVQDHLGQCVPVSLCPCVQDDKIYSPGTILQ-NNCNTCVCKHGAFNCTQEHCKSVQQC 756 HSP 3 Score: 139.813 bits (351), Expect = 1.734e-32 Identity = 132/495 (26.67%), Postives = 210/495 (42.42%), Query Frame = 3 Query: 1590 QCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGR---------CICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDI-ILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSC-DNTPQSIVDPPAGDNDICS-----TILNGPEFSGCMNSSKVDKEFYATYCRREQ---SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDD-DCFPGCECPPNYYKTGTGQCVSESECTC-WNPSRPKDPEIPAGTNITINCQTCVCINGRLNCK 3005 QCPG+ ++S C +TC +L Q+S V + C+C G +G C+ P+ CPC ++GQ + DCNTC C WHCT +C C G + TFDG +++F CQYV + + +F I N S + K VT + + I +L+ + V ++G T LP + + + K+ ++ + L +DG +Y+ ++ L+ + GLCG FD ++ENDFT S+ + S+ ++ SC D Q + DP + + +L FS C ++V + Y +C + S D C+AI + A C R G+ + Q PL G +++ C C +C + + + S+C+ C GC CP G G C+ +C C W S P GT+IT CQ C C+ G C+ Sbjct: 755 QCPGSLVYS--PRSCMLTCSSLDHQGQQ--QSSSVTKPSCREPLSGCVCPQGTFFHGDRCVLPDECPCHHNGQVYYTNETITKDCNTCVCQKRRWHCTRLVCDGVCVATGDPHYVTFDGRRYSFLG-DCQYVLA-------TETSGLFSITAENVPC------GSTGVTCTKSVTFSLGSTIIHLLRGKA---------VTVNGMPVT--LPKSYSGSGLTLEKV-GLFVSLSSRLGVTLMWDGGMRVYVHLAAHLRGQVGGLCGNFDEDTENDFTTHQGSVESTAEVFGNSWKVSPSCPDVENQDLQDPCLNPHRVTWAKKRCAVLTQDLFSVCH--TEVPFQHYYDWCVFDTCGCDSGGDCECLCTAIASYAEECNRRGV-----YVRWRSQELCPLQCEKGMIYEPCGPACSQSCPSVQQHSYSRCEVLSCVEGCFCPAGTIHHGDG-CILPLDCPCEWEGSM-----FPPGTSITQRCQNCSCVEGLWQCE 1206 HSP 4 Score: 111.694 bits (278), Expect = 7.175e-24 Identity = 101/384 (26.30%), Postives = 168/384 (43.75%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENC---TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFK-GFGCPDIAN--TLSTC-KPDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQN-SKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPST 1877 C + G +Y TFD + Y++ G C Y+L ++ S + +I EN ++ C K + G ++ +L P+++ + S G+ + + + F+ LS G + L WD +++ + + G V GLCG ++ ++ S FGNS+K CPD+ N C P A ++C+ + F C V Y+ CV + C D + LCT +++Y++ C+ +G ++WRS LC QC I+ C C +C ++ Q + C+ S V C C +G + +G CI P CPC ++G PPGT C C+C LW C CP++ Sbjct: 855 VCVATGDPHYVTFDGRRYSFLGDCQYVLA--TETSGL---FSITAENVPCGSTGVTCTKSVTFSLGSTIIH-----LLRGKAVTVNGMPVTLPKSYS-----GSGLTLEKVGLFVSLSSRLG-VTLMWDGGMRVYVHLAAHLR-GQVGGLCGNFDEDTENDFTTHQGSVESTAEVFGNSWKVSPSCPDVENQDLQDPCLNPHRVTWAKKRCAVLTQDLFSVCHTEVPFQHYYDWCVFDTCG--------CDSGGDCECLCTAIASYAEECNRRGVYVRWRSQELCPLQCEKGMIYEPCGPACSQSCPSVQQHSYSRCEVLSCVEGCFCPAGTIHHGDGCILPLDCPCEWEGSMFPPGTSITQRCQNCSCVEGLWQCEGVACPAS 1213 HSP 5 Score: 93.9745 bits (232), Expect = 1.470e-18 Identity = 77/260 (29.62%), Postives = 116/260 (44.62%), Query Frame = 3 Query: 1506 CTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLF----QTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGT-QFDTFD-------GAKFAFNDVGC--------QYVAVEPSVNNTDDRANVFVIIQSNTESMLG-TEGNSAYINEPKIVTVKVNGSDIILKA------TSPQLGDSQTNVFIDGEDHTTKLPFTIP 2204 C L+ Y+ C+ + I WR+ +LC CP +++SDC S CP +C + A C+ VG C C G + TC+K E CPC + + PG Q CNTC C S W CT + C C ++G Q TFD G F F + C Q + V S ++ F+++Q+ +L +G A I+ + KV+G L T+P+ GD + +V E ++ P T+P Sbjct: 305 CDTLATYARECAQQHIIIMWRTPSLCGRVCPRGQVFSDCVSSCPPSCASPHPPGPAATMAQCREECVGGCECPPGLFLHQGTCLKAEDCPCFHRRLTYQPGDSIQQRCNTCVCRSGQWQCTGERCAGQCTLVGALQVTTFDKKKYSLQGGDFLFQLLKCTVTVNGVRQTLPVVSSDLAVHRASSSFILVQTFGALVLWHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPE-GDVEMSVSAFAEKFISQ-PCTLP 562 HSP 6 Score: 90.5077 bits (223), Expect = 1.724e-17 Identity = 98/391 (25.06%), Postives = 148/391 (37.85%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTL--MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKY-----EDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGC----NRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTT---HCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCT 4439 C + PH Q S G C T + PG ++++ PI + + G S V I T+ LFP + I + L F V +G+R+ +D VYL + +GLCG Y+ + + + T+ + DA + S D+ + AE +C + + F C D Y CLY C GG C+ +LAT+A +CA I + WRT +LC +C G+ + C S CP +C + P+ CV GC CP G+ G C+ CPCF+ +Y G I+ + C C C ++ CT + C CT Sbjct: 101 RGCCEGWAGPHCT----QGVSLYGRCFSTWNCEEFPGLHNSSLMPIEKCCSTLW-GLSWKNTWGLVHKI--------------TV-LFPLW-ISVHWLGDFV-FVESGLGVRLKFDLINTVYLTVTAEHLAATRGLCGVYNNNPEEASLTETVSWLQQDCSDAAELGHSCDIVEDL------------------------TLRRQAESVCHQLLENP--FTHC--HFRVDPAAYIDTCLYLYCSLPTRERDGGACD----TLATYARECAQQHIIIMWRTPSLCGRVCPRGQVFSDCVSSCPPSCASPHPPGPAATMAQCREECVGGCECPPGLFLHQGTCLKAEDCPCFHRRLTYQPGDSIQ-QRCNTCVCRSGQWQCTGER--CAGQCT 434 HSP 7 Score: 79.337 bits (194), Expect = 4.604e-14 Identity = 73/305 (23.93%), Postives = 126/305 (41.31%), Query Frame = 3 Query: 1026 LSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXX-----XXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQI--------LANQQCSKIR-LKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGR------CICGSGEVE-YGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPST 1877 ++V L + + + V R + +L G + L W + + V GLCG + + + +V++F F C TL P D A + CS I F C D+V + YF +C+ +C + + CT L+AY+ C+ +G + WR+ T C QC G +++ +C C +C + + ++G+ C C +G V+ + C+ CPC+ D + + PGT+ Q +CNTC C ++CT++ C S Sbjct: 465 VTVNGVRQTLPVVSSDLAVHRASSSFILVQTFGALVL-WHLDGPFALIHLQPGLAHKVHGLCGTLTWSQHDDFTTPEGDVEMSVSAFAEKFISQPC-----TLPKAAPSDPCSTYTQRISYAERVCSVIHNPVFQKCHDVVEKEPYFRLCLSEVCACALQRS----------CHCTVLTAYAQHCAQEGVQVHWRNQTFCPVQCSGGQVFRECGRTCGFSCFDWQHGWLCNDSSGEIGQRVCVPGCQCPTGLVQDHLGQCVPVSLCPCVQDDKIYSPGTILQNNCNTCVCKHGAFNCTQEHCKSV 753 HSP 8 Score: 61.2326 bits (147), Expect = 1.287e-8 Identity = 76/314 (24.20%), Postives = 121/314 (38.54%), Query Frame = 3 Query: 2109 ILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSEN-DFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRR---EQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELN----EATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTN 3026 I K S G S N + T P I ++H + V+ +L FD ++Y+ V+ GLCG ++ N E TE + +++ + A+ + SCD + A +C +L P F+ C +VD Y C + + C + A C Q + C +C G VF C +C +C + AT ++C ++C GCECPP + G C+ +C C++ P G +I C TCVC +G+ C T + C Sbjct: 142 IEKCCSTLWGLSWKNTWGLVHKITVLFPLWI---SVH---WLGDFVFVESGLGVRLKFDLINTVYLTVTAEHLAATRGLCGVYNNNPEEASLTETVSWLQQDCSDAAE--LGHSCDIVEDLTLRRQA--ESVCHQLLENP-FTHCH--FRVDPAAYIDTCLYLYCSLPTRERDGGACDTLATYAREC----AQQHIIIMWRTPSLCGRVCPRG-QVFSDCVSSCPPSCASPHPPGPAATMAQCREECVGGCECPPGLF-LHQGTCLKAEDCPCFHRRLTYQP----GDSIQQRCNTCVCRSGQWQC-TGERCAG 431
BLAST of SCO-spondin vs. Ensembl Medaka
Match: vwf (von Willebrand factor [Source:NCBI gene;Acc:101162365]) HSP 1 Score: 342.043 bits (876), Expect = 1.791e-94 Identity = 350/1309 (26.74%), Postives = 551/1309 (42.09%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVV-VKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPM--YVLRQDNF-ILLSLE-AGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFG----CPDIANTLSTC---KPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQSIVDPPAGD-------NDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKD-QLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATAS-KCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKD------------------NCTNPVCPKNQVYMDSVSG------CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTP--GDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIV--IEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYE-DAIQFAESFIAN---ADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGK-------------HYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCS--CPNGMI--DLNGECVPPTTCP-CFYNGQ--SYVNGAIIKMEN---CKKCQCVQAKFNCTEDYSDCITTCTPS 4445 C +G + TFD LY + G CSY+L D S ++++ + N TG + + GD + S +G L + L P + S + G+ + Y L + F ++++ A NI L ++ GLCGN + +++++ + + + F NS+ G C ++N +C K + IL++ K F CA LV + + +C C +Q + D C L Y+ +C G + W ++ C +CP +S+C C TC +L C++ + C C +G+V G C++ C C + G+ PPG+ DCNTC C W CT + CP C ++G + F +FD F F CQY+ + VNN F +I N + N+ + T V S L+ + +L V ++ D T + + ++H+++ + S +L +DG + +K++ GLCG ++GN +DF + +++ I G C++T + DP A + + C+ I++ FS C V+ + +CR + + D + CSA+ A +C G+ V + S +L C C +GG V++ C C TC L+ A A + + C GC CP Y + +GQCV+ C C + + P + +I C C NG + C + + + CP V + SG +C N D + S C CP DTV + C+ P CPC + NR YA IT C +C NR W + C+ VC + G+ HY T DG FSF G C Y L+ G+ + + V N ACG CAK V + G ++ G P+ V I F+T L+ I L WDR TR+ + + +++G V GLCGN+DG++ N++M + E D+ F S+ AD+ + C+ N + EQ C +M + +F C + D Y C+ AC C GDC C C +A +A C+ GI + WR+ +LCP+ CE Y+ C CP TC P CS T CVE C CP G + ++ CV P+ C C +NGQ S+ N I+ +N CK C C +C ED + TP+ Sbjct: 27 CSLFGRYHVHTFDGVLYEFPGDCSYLLAG--DCSHRSFTLLGDFHN-GKRTG----VTLFLGDAFELHLSVDGFLSQREKRLTL-PYA-----SHAVFAGSELGFYKLWSEEFGFTVTIDNAANIALTLSKHHA-----------NRTCGLCGNFNAVTADEYTAQEGLLTEDSYDFANSWAVKGADQSCRRVSNPSKSCNSTKGTEAILSSCSVLKTSGVFLHCAHLVPPEAFLLLCQAEACHCNQRD--------VEDCYCPILLEYARTCHAHGILLHGWLEESKCYPKCPIGMQYSECTKSCSTTCHSL--NIQEVCKDECMDGCTCPAGKVLDGNRCVEVSECSCTHMGRHFPPGSTVSQDCNTCVCRHGSWECTNEGCPGECLVVGQSHFKSFDNKFFTFTG-DCQYLLAKDCVNNE------FSVIIENVQ---------CADNQDAVCTRSVTLSLPSLENMTVKLKHGGV-VSVNNMDIQTPMHY----GHLHIQRAVQSSVRLVFSDDLRLDWDGQGRVLLKLTARWTGQTCGLCGNYNGNQGDDFMSDSGLVETKPQAFGQAWKINGDCESTHKYDPDPCAINPKRVRFAEEACAVIMSD-VFSPC--HILVNPSPFQRFCRYDVCACGDGEKCLCSALAAYAASCSAKGVL--VNWRSALL--CELEC-SGGQVYEACGTVCDRTCLSLSGAEAGCEGEKVCEEGCFCPVGKYLSPSGQCVTADLCPCLHDGQLYQPNDVYADHTSI----CYCENGSMRCSSSEVPSLLSDLFYDEDLGSTRERRSAHCPPPLVRTECGSGKKGLECARTCQNLDLPCVSLSCIPGCLCPPDTV-RHRRDCIAPEQCPCYHNNRPYAAGQTITTDCNTCVCENRKWRCTQKL---CDGVCRSVGEGHYITFDGLKFSFPGLCQYVLVQDMCLGEEGS----FRVLVENEACGIVGRRCAKAVTVFYQGGLIVMQHGEVTMKKPVPQSSQVEIIRSGQFYTLLLGKHISLS--WDRGTRLLVHISAAYRGRVCGLCGNFDGNVNNDFMSSNNQLEVDSSHFGNSWKVIPSCADVTQIPLP-------------CSDNIMKLVTVEQSCRLM--SGPLFKECNTQV--DAEPYVEMCVEAACSCPSVGDCACFCDVVAAYAQACSERGISISWRSNDLCPMSCEELNPKISGVGEELCQWRYNTCGPACPITCQ---HPEPLQCSFT-CVEACHAYCPPGQLLDEVAMRCVNPSQCQMCVHNGQRISHGNKVILNHDNPALCKICHCENNSLSC-EDCTSLSMLSTPT 1236 HSP 2 Score: 107.071 bits (266), Expect = 1.720e-22 Identity = 110/417 (26.38%), Postives = 172/417 (41.25%), Query Frame = 3 Query: 3297 TANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESF-IANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTED--YSDCITTCTPSQFRCDDGKCIESSKICDKV--TDCSNSE 4523 A C +G H T DG ++ F G C Y L GD S+ + N G +L F ++ + +L R T+P +L F+ +L S+ G V D + + L L GLCGN++ +EY + ED+ FA S+ + AD +S PSK CN+ ++ C V+K T+GVF C + + + C +AC CN+ +C C L +A C GI + W ++ C C G Y C C TC ++ N C C++GC+CP G + CV + C C + G+ + G+ + ++C C C + CT + +C+ S F+ D K + C + DC N+E Sbjct: 20 VAGVTGRCSLFGRYHVHTFDGVLYEFPGDCSYLLA---GDCSHRSFTLLGDFHNGKRTGVTLFLGDAF-ELHLSVDGFLSQREKRLTLPYASHAVFAGSELGFY-KLWSEEFGFTVTIDNAANIALTLSKHHANRTCGLCGNFNAVTADEYTAQEGLLTEDSYDFANSWAVKGADQSCRRVS-----NPSKS---CNSTKGTEAILSS-CSVLK-TSGVFLHCAHLVPPEA--FLLLCQAEACHCNQRDVEDCYCPILLEYARTCHAHGILLHGWLEESKCYPKCPIGMQYSECTKSCSTTCHSL--NIQEVCK-DECMDGCTCPAGKVLDGNRCVEVSECSCTHMGRHFPPGSTVS-QDCNTCVCRHGSWECTNEGCPGECLVV-GQSHFKSFDNKFFTFTGDCQYLLAKDCVNNE 414 HSP 3 Score: 71.2478 bits (173), Expect = 1.084e-11 Identity = 88/367 (23.98%), Postives = 148/367 (40.33%), Query Frame = 3 Query: 768 TCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVI-EAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIP-GTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGF-GCPDIANTLSTCKPD--DQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISD--VLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCP---------GNKI----WSDCASECPVTCRNLFQTPSAY-CQNSKVGRCICGSGEVEYGYTCIKPESCP-CLYDGQE 1784 C S G +Y TFD +++ G C Y+L V + C + G +V+V EA +V + + T L + +P + + ++R F L L +I L WD + ++ Y G V GLCG ++ S++ + + FGNS+K C D+ C + + Q C + F C V+++ Y MCV+ C+ PS+ D C ++AY+ +CS++G I WRS+ LC C G ++ ++ C CP+TC++ ++ C + C G E C+ P C C+++GQ Sbjct: 857 VCRSVGEGHYITFDGLKFSFPGLCQYVL---------------VQDMCLGEEGSFRVLVENEACGIVGRRCAKAV--TVFYQGGLIVMQHGEVTMKKPVPQSSQVEIIRSGQFYTLLL-GKHISLSWD-RGTRLLVHISAAYRGRVCGLCGNFDGNVNNDFMSSNNQLEVDSSHFGNSWKVIPSCADVTQIPLPCSDNIMKLVTVEQSCRLMSGPLFKECNTQVDAEPYVEMCVEAACS----------CPSVGDCACFCDVVAAYAQACSERGISISWRSNDLCPMSCEELNPKISGVGEELCQWRYNTCGPACPITCQHPEPLQCSFTCVEACHAYCPPGQLLDEVAMRCVNPSQCQMCVHNGQR 1194 HSP 4 Score: 63.929 bits (154), Expect = 2.147e-9 Identity = 98/438 (22.37%), Postives = 170/438 (38.81%), Query Frame = 3 Query: 3222 PNDVITIKCAKY--------ICSNRMWNLLSEETANCE----AVCIAYGDPHYKTLDGQMFSF--QGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLA---TCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLF--------LKPSFQGNV-----QGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHC--------VEGCSCPNGMIDLNGECVPPTTC-PCF-YNGQSYVN-GAIIKMEN-CKKCQCV-QAKFNCT 4406 P+ +T++ K C N M + ++T +C +C+ H D +G C YTL+ DS + + C + + C K +++ G +L +D + + ++ + + +L+ G +L +S + Y+ +FQG+ GLCG + N +++ + +FI++ + + E+ V K Q +C S A C + VF C + V + + C AC + C ++ +A C G+ V WR+ +LCP+ C YDAC + C + CD+I + PS S EGC C G + +G CV P C C +G +Y + + + EN C C C+ Q + NCT Sbjct: 1924 PSGAVTVQSHKVSCDKLEPPACRNNMAPVKVQDTCSCHWECPCMCMGSSTNHVVRFDSLALRLDGEGLCSYTLLTVNSDSGEGS---EVKLHTGPCQETTSYNQICMKAMEVTHGPHKLIL---RDDMRAMIDREELALPWRFYGMELMR--YGGVMLQLKSNQGYMLTFTPQSNEFTITFQGSTNSAQTAGLCGACGEEKVNIL---SLRNGSSTTNQPAFISD-----WTAAGEDGVCLPKRQSVC------LSGAAMGCQALHSE--VFLPCHAFI--PVQIFLTKCEEQACEESDA------CEVISAYARLCRQRGVCVDWRSPHLCPLKCPSSMEYDACRTGCVEDCDSIQA-LPSDWSVARSNESCMDTPTEGCFCAGGTVLHHGRCVSPEACGQCVDQHGHTYTHMQSWVPDENPCLICMCLDQQRINCT 2328 HSP 5 Score: 55.4546 bits (132), Expect = 6.469e-7 Identity = 30/102 (29.41%), Postives = 46/102 (45.10%), Query Frame = 3 Query: 1506 CTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAY--------CQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEH 1787 C +SAY+ C +G C+ WRS LC +CP + + C + C C ++ PS + C ++ C C G V + C+ PE+C D H Sbjct: 2198 CEVISAYARLCRQRGVCVDWRSPHLCPLKCPSSMEYDACRTGCVEDCDSIQALPSDWSVARSNESCMDTPTEGCFCAGGTVLHHGRCVSPEACGQCVDQHGH 2299
BLAST of SCO-spondin vs. Ensembl Medaka
Match: otog (otogelin [Source:NCBI gene;Acc:101158818]) HSP 1 Score: 287.345 bits (734), Expect = 1.716e-77 Identity = 243/890 (27.30%), Postives = 405/890 (45.51%), Query Frame = 3 Query: 1593 CPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVG---RCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQF-DTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSK-SIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGS-------------CDNTPQSIVDPPAGDNDICSTILNGPEFSGC---MNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPP-NYYKTGTGQCVSESECTCWNPSRPKDP-EIPAGTNITINCQTCVCINGRLNCKTKDN-CTNPVCPKNQVYMDSVSG---------------CNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP-----LFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHG-TMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLC 4127 CP + +C S CP TC +L +T C +S++ C C G + C++ CPC + G +P G + Q +CN CTC +W+CT+ CP C + G F +FDG F F CQYV V+ ++ V IQ+ + G + + A I +V + +++ L +G+ VF+ G+ + LP+ D H+++L++++ +V+ + +L + ++ +Y++ + K++ +GLCG F+GN ++DF I + S+ S CD+ Q++ + + +C +LN F+ C ++ + ++ A CR CS + + A CR+F + V F S+I C C A + C +CQ C L A +C ++C GC CP ++Y T CV S+C C +P ++PA Q + G L C +CP Q+Y + G C S C+ S + C CP+ + D C +P+ CPC++ +EY P D ++ C + C + + C ++C AYGD HY+T DG +F + G+C L+ + D+ +++ N+ C + C K++ I +G LLS ++ P + + I Q FT + + VLWD T V++ + P +QG + GLCGN+D NE M +F S+ A + +I + C+ NP R+ +A+ C V+ + VF +C + DV+ +Y NCL D C C+RGGDCEC C+S+A +A +C G+PV WR+ +LC Sbjct: 413 CPAGLQYRECISCCPATC-SLERT----CIDSRLACLDGCYCPDGLIFQDGGCVEASDCPCEHHGVFYPAGQMLQEECNNCTCVGGVWNCTDHSCPGECSVTGDMFFQSFDGRIFTF-PASCQYVLVKSRLS-----GKFTVTIQN---APCGPDLDGACIQSVSLVVDEDPRTEMTLS----HMGE----VFMAGQ-YRISLPYY--DDVFHIQELSSMFLQVRTAFGLQLQYSWAEFRLYLQAEERWKDDTVGLCGTFNGNIQDDFLSPSGMIESTPQLFGNSWKVSSACSSSLSVSPIDPCDSHQQAV----SYASSMCD-VLNQDLFAACHDYLSPAPFHQQCRANTCR------CGTPCLCSTLAHYARHCRKFSVI--VDFRSHIPD-CAVSCPAT-MEYSSCVSSCQRRCSSL--AALQRCGEECEEGCACPQGSFYNHRTHTCVHRSQCPCSLFGADYEPGDLPA--------QLSWSLTGFLKCSGFSFFLPERLCPAGQLYQNCSDGDDAFLPGRGVACERTCESHLLNIPCSSHDSCVSGCVCPHGLLKHGD-ECFEPAACPCLWKGKEYFPGDRVSSPCYQCACQDGTFQCAFRP---CPSMCTAYGDRHYRTFDGLLFDYVGACKVYLVKSSADAV-----FSVTAENVDCFDTGVICKKYLLINVGAS--LLSLDDHMGKPNPSSVIDRNQRLFIWQAGLFTVVHFPEEDITVLWDHKTTVHVQVGPHWQGRLSGLCGNFDLKTVNEMRTPENMDSPTPQEFGNSWTATECVNSPDI-----------RHPCSLNPLREPYAKHQCAVL--LSEVFQTCHPVV--DVSWFYENCLADTCACSRGGDCECFCSSVAAYAQRCCHEGVPVDWRSPSLC 1226 HSP 2 Score: 129.798 bits (325), Expect = 2.041e-29 Identity = 115/463 (24.84%), Postives = 204/463 (44.06%), Query Frame = 3 Query: 3027 PVCPKNQVYMDSVSGC-NSCTNYDTCTDTS-SIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSL-ATCAKFVQIIIG------------GQTYLLSRGNNDTIPLFPKYDIV--IEQLTFFTQLVSDSIGLRVLWDRST-RVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQ-FAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNL-CPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMI--DLNGECVPPTTCPCFYNGQSYVNGAI 4349 P CP Y + +S C +C+ TC D+ + + C CP+ + + DG CV+ SDCPC + Y ++ +C C +WN +C C GD +++ DG++F+F SC Y L+ + T+++ N CG L C + V +++ G+ ++ + P YD V I++L+ V + GL++ + + R+YL + ++ + GLCG ++G++Q++++ + E Q F S+ ++ ++ D C+++ S+A MC V+ +FA+C + + ++ C + C C CLC++LA +A C + V +R+ C + C Y +C S C + C ++ A C C EGC+CP G CV + CPC G Y G + Sbjct: 411 PPCPAGLQYRECISCCPATCSLERTCIDSRLACLDGCYCPDGLIFQ-DGGCVEASDCPCEHHGVFYPAGQMLQEECNNCTCVGGVWNCTDH---SCPGECSVTGDMFFQSFDGRIFTFPASCQYVLVKSRLSGK-----FTVTIQNAPCGPDLDGACIQSVSLVVDEDPRTEMTLSHMGEVFMAGQYRIS----LPYYDDVFHIQELSSMFLQVRTAFGLQLQYSWAEFRLYLQAEERWKDDTVGLCGTFNGNIQDDFLSPSGMIESTPQLFGNSWKVSSA--------CSSSLSVSPIDPCDSHQQAVSYASSMCDVLN--QDLFAACHDYL--SPAPFHQQCRANTCRCGT----PCLCSTLAHYARHCRKFSVIVDFRSHIPDCAVSCPATMEYSSCVSSCQRRCSSLA--ALQRCGE-ECEEGCACPQGSFYNHRTHTCVHRSQCPCSLFGADYEPGDL 841 HSP 3 Score: 97.4413 bits (241), Expect = 1.333e-19 Identity = 96/383 (25.07%), Postives = 161/383 (42.04%), Query Frame = 3 Query: 3309 EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVAN-IACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP------LFPKYDIVIEQLTFFTQLVSDSIGLRVLWD-RSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM--HGTMKYEDAIQFAESFIANADICPYEISPENNVAP---SKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASC---IEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTE 4409 E C +G +++T DG + F G C YTL+ ++I V N C + TC + V I + + + +N T + + +EQ++ + LV+ G + W+ R+ VY+ L P F G GLCGN++ ++Q++ +G + + D F S++ E+ P N P S P E++C ++ + F SC + + + Y+ Y F C F + +++ P C G Y C S CP TC + S + C++GC CP+G+I +G CV + CPC ++G Y G +++ E C C CV +NCT+ Sbjct: 158 EMTCRTWGQYNFETFDGLYYYFPGRCSYTLLK-----DSHISFFVFKVHNDPECSSAPYTCQRSVSIFLPWEGEIRLHASNVTFKGRRLQLPHHLHHLQLEQISQYV-LVTQQQGFTLAWEGRTGSVYIKLSPDFVGGTCGLCGNFNAEVQDDMTTSYGLLTH-DVEMFGNSWM--------EMDPHQNTCPPVGSSFPSPYRPFPVSVQRVEEVCSML--LSPPFQSCHDFVSPLPYMASYYWKKDYYR------------------IFFGTCFFCDASLFLPPRSVPP--CPAGLQYRECISCCPATCSLERTCIDSRLA---CLDGCYCPDGLIFQDGGCVEASDCPCEHHGVFYPAGQMLQ-EECNNCTCVGGVWNCTD 499 HSP 4 Score: 64.6994 bits (156), Expect = 9.851e-10 Identity = 74/283 (26.15%), Postives = 120/283 (42.40%), Query Frame = 3 Query: 771 CHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCT-SDTG--CVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGC---PDIANTLSTCKPDDQILANQQCSKIRLK-FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLC 1583 C +YG +YRTFD L+ Y G C L S AV ++ EN DTG C K ++I G +L D P SV + + L ++ + F ++ +I + WD + + G ++GLCGN D N+ +N+ S FGNS+ C PDI + S P + A QC+ + + F C +V+ ++ C+ + C S+ + + C+ ++AY+ C +G + WRS +LC Sbjct: 973 CTAYGDRHYRTFDGLLFDYVGACKVYLVK-SSADAV---FSVTAENVDCFDTGVICKKYLLINV--------GASLLSLDDHMGKPNPSSVIDRNQRL-------FIWQAGLFTVVHFPEEDITVLWD-HKTTVHVQVGPHWQGRLSGLCGNFDLKTVNEMRTPENMDSPTPQEFGNSWTATECVNSPDIRHPCSL-NPLREPYAKHQCAVLLSEVFQTCHPVVDVSWFYENCLADTCACSRGGD--------CECFCSSVAAYAQRCCHEGVPVDWRSPSLC 1226 HSP 5 Score: 55.8398 bits (133), Expect = 5.813e-7 Identity = 88/375 (23.47%), Postives = 145/375 (38.67%), Query Frame = 3 Query: 3303 NCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDS---------SNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGD------LQNEYMHGTMKYEDAIQFAESF-IANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDV--NRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPC-PQTCDTIGSNA-PSHCSTTHCVEGCSCPNGMI---DLNGECVPPTTCPCFYN-GQSYVNGAIIK 4355 +C C + D + T DG + + +Y + P ++ +++ + + N+ C SL + ++I L F KY I T L+ GL++ W ST + + ++ + GLCG DGD L N + G ED F +S+ ++N + ++ S+ +D+ + D + C M + N F +C H V + + + D+ N C +LA + C I ++WR N CP +C Y AC C Q+C +A P CS EGC CP G + + C+PP C C + G NG + K Sbjct: 2114 DCPCRCSMFPDLNVITFDGNSVAIYKAALYIVTQLPNETVTVMVQECPADSESLLLWNFTNL-CLVSLNITHQSNHVLINRLQRRLYVNTRYAKARFKKYGFEIYD-TGNMYLIRSPAGLKLQWYHSTGM-MVIETDSKVPTMGLCGVCDGDPTDDLTLPNGSVLGAG--EDPALFIDSWQVSNTT---------SYISQSRRRDLNCSTSDCST-----CLSMLE-NATFTAC----HAFVPPSTFCEVWVRDSEYVNNQ------CVALAAYVASCHKFNICIEWRRPNYCPFICAATLRYQACLPACTSQSCPNHDFDADPEQCSGL--TEGCVCPEGTLLHRQYSALCIPPEKCACTDSAGVPRANGEVWK 2456 HSP 6 Score: 55.0694 bits (131), Expect = 1.012e-6 Identity = 44/147 (29.93%), Postives = 62/147 (42.18%), Query Frame = 3 Query: 4134 MCEGGKHYDACAS-----------PCPQTCDTIGSNAP--SHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTP---SQFRCDDGKCIESSKICDKVTDCSNSED 4526 +C G+ Y C+ C +TC++ N P SH S CV GC CP+G++ EC P CPC + G+ Y G + C +C C F C + C + CT +R DG + C KV +S D Sbjct: 866 LCPAGQLYQNCSDGDDAFLPGRGVACERTCESHLLNIPCSSHDS---CVSGCVCPHGLLKHGDECFEPAACPCLWKGKEYFPGDRVS-SPCYQCACQDGTFQCA--FRPCPSMCTAYGDRHYRTFDGLLFDYVGAC-KVYLVKSSAD 1005
BLAST of SCO-spondin vs. Ensembl Medaka
Match: FCGBP (Fc fragment of IgG binding protein [Source:NCBI gene;Acc:101155957]) HSP 1 Score: 234.958 bits (598), Expect = 1.836e-61 Identity = 302/1279 (23.61%), Postives = 495/1279 (38.70%), Query Frame = 3 Query: 759 NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRL---------KFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKG-SCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPS--AYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEH-------PPG--------------TVRQFDCN---TCTCNSSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTE--SMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLY--FDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG----------------SCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDP-EIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTC-VQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIP-----LFPKYDIVIEQLTFF----TQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMK-YEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNAN------PDRKSWAEQMC--GVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNG 4343 +++TC + G +Y TFDN+ Y +QG C Y L A ++ +N V + V +K IL N+ L L V+ + + + + + + +F L + W + + + + G V GLCG D+ + + FG S++ P P I Q K L F C V+ +F CV ++C DVLC ++AY+ +C G + WR+ C +CP + + CAS CP +C L P A+C+ C C G + G C+ C CLY+ + P G T ++F C C + + C + C G + +FDG KF F C YV + S D F + N + M G + ++ ++V V+V G +I+K+ V ++G +P + D + V + Y ++++F L +D + + V ++ + GLCG F+G+ ++DF D + + I KS+ CD + + PA C IL P+ + SK+D + Y C + +SN D C ++ A C G+ T + P+ + +++C+ TC +C L++ +C + C GC C + G CV E+EC C++ + P E+ + ++ C N T C CP++ M G C N D C D + ++ TC V+ + CI KY + C A+GDPHY T DG F FQG+C Y + T G+ N + P +I+ N G + + + V + I + + + L + + +++ F + +V GL V +D + + + L S+ +V GLCGN++GD +E + + K I++ +S ++ ++ P D C N +R+ + Q G+ TN VF C + D + +NC+YD C N+ GD LC +L ++ QC GI +K WR + CP+ C+ HY+ CASPC +C CS CVE C C G + G C+P TC C Y G+ Y G Sbjct: 436 SYSTCKATGDPHYVTFDNKKYDFQGTCVYQLAALCSKDTELTPFEVLVQNEHRGRNVVSYTKL----VQIKVYSLNIL-----NDQLVNLPVSLVEGKVSVYKSGWFAVVTTDFGL------KVSFNWHSAAFVTLPSNYM---GAVCGLCGNYNGNMQDDLTPKNGNTPVKPTDFGTSWRVAEIPGCIEGCQGVCPSCDITQKVQYEKEDLCGIIRDTKGPFRDCHAKVDPAGFFEDCVYDVCMYEGRK----------DVLCQAVTAYTSACQGVGVNVYSWRTSNFCAVKCPIHSHYDICASACPASCEGLAPPPGCRAHCEEG----CSCDEGYILSGDQCVPFSQCGCLYNDLYYRINEVFYPNGQCEEECKCTQDGEVTCKKFKCGPNEKCKIENGIQKC-HPVGKGVCRASGDPHYTSFDGQKFDFQGT-CTYV-LSKSCGVEDTHLEAFSVQVENVQWDRMKGKKK----VSVTRLVAVEVFGFTLIMKS-------KMFGVLVNGV--LNNIPLNLNDGEVQVYQEGTNYL---IATNFGLLVTYDLVYHVTVTVPGNYRDKVCGLCGNFNGDRKDDFQMPDQILAKDINVFGKSWKVAIPNVVCENGCQGNECADCDPAHKDVFSKPA----YCG-ILTAPKGPFAVCHSKLDPQSYFDDCVFDVCASNGDGKVLCDSVAAYAYNCHMAGVNVNDWRTPSFC----PMKCPANSHYEVCAETCTYSCPGLSDMV--QCPEACTEGCVCDAGFLYDGQ-TCVKETECGCYDRGKTYKPGEVTYEEDCSLACS--------CNPATGLICEEYSCPEDTQCMIK-KGVKECYNKDPCKDANC-------------RVQETCRVEGGEAVCI----------------PKYT-----------------STCWAWGDPHYHTFDGFDFDFQGTCKYIISKTCGN-LNGLVPFSITERNDNRGNAAVSFVREVDVFIYDYNITIRKNQVGQVTVNGELLNLPFQLGDNEVSVFQSGSSAVVETKFGLIVSYDWNWHLVIKLPSSYYSSVCGLCGNFNGDTGDELQNPSGKAVASVIEWGKS---------WQTPDQDKGHPC--WDTCEKNCPTCDDDERELYKTQAFCGGLAAKTNSVFKICNSKL--DPQAFMNNCVYDMC-LNK-GDKRMLCQALTSYNKQCRDEGIIIKDWRKEFNCPMNCQRFSHYEHCASPCQPSCPF--PEQKQTCSGA-CVEACVCDEGYVLSAGVCIPAKTCGCSYQGRYYTPG 1577 HSP 2 Score: 162.155 bits (409), Expect = 2.669e-39 Identity = 126/444 (28.38%), Postives = 188/444 (42.34%), Query Frame = 3 Query: 3165 GTCVQPSDCPCIYMNREYAPNDVI-----------------TIKCAKYICSNRMWNLLSEETANCE----AVCIAYGDPHYKTLDGQMFSFQGSCMYTLM----DTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN----NDTIPLFP--KYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGD-LQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKS---WAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMEN--CKKCQC 4382 G C+ C C Y R Y P + C + CS ++ + C+ A C A GDPHY T DG+ F+FQG+C+Y L PG + P ++V N G + K V I G T +S+ N N + P D + L+ ++ + G+ + +D + V + + ++ G + GLCGNY+G L + M D + ES ++++ + + + C A D + A Q CG++ D G F C + + D Y +C+YD C G +C ++ + + C GIP+ WRTQ CP+ C HY+ CAS CP TC +I S H HC E C C G + CVP C C YNGQ Y G + E+ +KC C Sbjct: 1557 GVCIPAKTCGCSYQGRYYTPGQQFWADEACSRLCVCDTTLGMVTCREASCSVNEKCIIQDGERVCQPISYATCTASGDPHYHTFDGRRFNFQGTCVYQLAGLCSQHPG-----LVPFNVTVENENRGSKAVSFTKTVTFSIYGSTLTISKENPYKLNGQLAALPLKSNDELAVFLSGSQAVIETAAGITITYDWWSTVRVTVPSNYHGAMCGLCGNYNGKALDDMTMQNGKTTPDEAKLGES---------WQVASVPGCSSACQGPWCQACSDSQKKVYQASQFCGIIADETGPFRECHKFV--DPAAYVEDCVYDVC--QFHGHHGSVCKAVEVYLSACQSQGIPIHPWRTQAFCPMECPANSHYNLCASGCPTTCASINSLTTCH---MHCSEACECDQGHLMSGDTCVPVRDCGCSYNGQYYQKGDVFYPEDTCMEKCIC 1979 HSP 3 Score: 160.229 bits (404), Expect = 1.129e-38 Identity = 131/477 (27.46%), Postives = 205/477 (42.98%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGC-NSCTNYDTCTDTSSIANSCGCPNDTVSKIDG-TCVQPSDCPCIYMNREYAPNDVI----------------TIKCAKYICSNRMWNLLSEETANC----EAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQM--CGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCK-KCQC 4382 CP N Y SGC +C + ++ T T + S C D + G TCV DC C Y + Y DV + C K C L C E C+A GDPHY + DGQ F FQG+C+Y L D+ N + P T++ N G + K V + + + +PL + V GL+VL+D V + + ++QG + GLCGNY+ ++E+ + G ++ +F +S++ + PE ++ E+D E++ CG+MK +G F +C + D Y S+C++D C GG E LC S+ + C G+ ++ WR+ + CP C HY+ CA C TC + CS + C EGC C G + +CVP C C ++G+ ++ ++C+ +C C Sbjct: 1899 CPANSHYNLCASGCPTTCASINSLT-TCHMHCSEACECDQGHLMSGDTCVPVRDCGCSYNGQYYQKGDVFYPEDTCMEKCICGENGAVSCQKEKCRRGETCKLVNGVKGCHPEEEGKCVASGDPHYTSFDGQRFDFQGTCVYVLAKVCDDNDNQLTPFTVTQGNAKYGNGKVSVTKSVTVTVNNEEL--------NLPLSLDKGRLTVTREGHNTFVRTDFGLKVLYDTVYYVEVIVPSTYQGKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSWVVDL--------PE-HICGGCEEDCPVCEKANAVLYEKLDSCGIMKAPDGPFQACHSKI--DPAAYVSDCVFDVCA--AGGSKEILCNSVQAYVLACQNAGVQIQPWRSSSFCPASCPPNSHYEVCADTCRGTCASFIQQLT--CSES-CFEGCECDAGFVFDGAQCVPLENCGCVHDGRYLTVNQVVVDKDCQSRCVC 2350 HSP 4 Score: 151.369 bits (381), Expect = 5.560e-36 Identity = 115/378 (30.42%), Postives = 182/378 (48.15%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPL---FPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYM--HGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSY-VNGAIIKMENC-KKCQCVQ------AKFNC 4403 + C A GDPHY T D + + FQG+C+Y L + + P + V N G ++ + K VQI + Y L+ N+ + L + + + + +F + +D GL+V ++ + ++ L ++ G V GLCGNY+G++Q++ +G + F S+ A+I P I V PS C+ + E +CG+++DT G F C + D ++ +C+YD C G + LC ++ + + C +G+ V WRT N C + C HYD CAS CP +C+ + AP HC EGCSC G I +CVP + C C YN Y +N C ++C+C Q KF C Sbjct: 438 STCKATGDPHYVTFDNKKYDFQGTCVYQLAALCSKDTE-LTPFEVLVQNEHRGRNVVSYTKLVQI----KVYSLNILNDQLVNLPVSLVEGKVSVYKSGWFAVVTTD-FGLKVSFNWHSAAFVTLPSNYMGAVCGLCGNYNGNMQDDLTPKNGNTPVK-PTDFGTSWRV-AEI-PGCIEGCQGVCPS-----CDITQKVQYEKEDLCGIIRDTKGPFRDCHAKV--DPAGFFEDCVYDVC--MYEGRKDVLCQAVTAYTSACQGVGVNVYSWRTSNFCAVKCPIHSHYDICASACPASCEGL---APPPGCRAHCEEGCSCDEGYILSGDQCVPFSQCGCLYNDLYYRINEVFYPNGQCEEECKCTQDGEVTCKKFKC 794 HSP 5 Score: 149.828 bits (377), Expect = 1.406e-35 Identity = 126/451 (27.94%), Postives = 197/451 (43.68%), Query Frame = 3 Query: 3171 CVQPSDCPCIYMNREYAPNDVI----------------TIKCAKYICSN----RMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIAC----GFSLATCAKFVQIIIGGQTYL--------LSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEY-MHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVK-WRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCK-KCQCVQAKFNCTEDYS 4418 CV S C C+Y + Y N+V + C K+ C ++ N + + + VC A GDPHY + DGQ F FQG+C Y L + G ++ ++ V N+ G + + V + + G T + L G + IPL V L++ + GL V +D V + + +++ V GLCGN++GD ++++ M + +D F +S+ + + EN ++ D A+ D S CG++ G FA C + D Y+ +C++D C N GD + LC S+A +A C G+ V WRT + CP+ C HY+ CA C +C G + C C EGC C G + CV T C C+ G++Y G + E+C C C A E+YS Sbjct: 748 CVPFSQCGCLYNDLYYRINEVFYPNGQCEEECKCTQDGEVTCKKFKCGPNEKCKIENGIQKCHPVGKGVCRASGDPHYTSFDGQKFDFQGTCTYVLSKSCGVEDTHLEAFSVQVENVQWDRMKGKKKVSVTRLVAVEVFGFTLIMKSKMFGVLVNGVLNNIPLNLNDGEVQVYQEGTNYLIATNFGLLVTYDLVYHVTVTVPGNYRDKVCGLCGNFNGDRKDDFQMPDQILAKDINVFGKSW----KVAIPNVVCENGCQGNECADCDPAHKDVFS-KPAYCGILTAPKGPFAVCHSKL--DPQSYFDDCVFDVCASN--GDGKVLCDSVAAYAYNCHMAGVNVNDWRTPSFCPMKCPANSHYEVCAETCTYSCP--GLSDMVQCPEA-CTEGCVCDAGFLYDGQTCVKETECGCYDRGKTYKPGEVTYEEDCSLACSCNPATGLICEEYS 1186 HSP 6 Score: 124.405 bits (311), Expect = 9.096e-28 Identity = 104/378 (27.51%), Postives = 160/378 (42.33%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPL---FPKYDIVIE--QLTFF----TQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGT-MKYEDAIQFAESFIANADICPYEISPENNVAPSKEQD------MCNANPDRKSWAEQMCGVM-KDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKM-ENCKK-CQCV 4385 C A GDPHY+T DGQ F F G+C Y + G+ S + + N G + V + + T R + + I++ +L F + ++ GL V +D + + + L ++ G GLCGN++G+ ++++ + + A+ F S+ P P D C + K ++ CG++ + NG F +C + D Y NC YD C G + LC +L T+A C G+ + WR C C Y+ C S CP TC +APS C CVE C+C G + ECVP T C C Y+G+ G +NCK+ C+CV Sbjct: 45 GTCWAMGDPHYRTFDGQRFDFMGTCTYVIAKNCGEDSR-LTAFEVHAQNENRGSHRVSYVGLVTVQVYDTTITAVRSETGRVRVDNSLWSLPIILNNGKLKIFQSGRSVVIEADFGLMVRYDWTHNLVVTLPDTYSGKTCGLCGNFNGNPKDDFTTPSGTQASGAVAFGSSWKV----------PGLVADPQCRNDCVGGCGSCTESQLNKWDSDLSCGIITRKENGPFKNCHAII--DPKAYLENCKYDL--CMGKGLRQFLCNALETYAEACQAAGVQIGDWRGLAKCSPRCPANSKYELCGSACPATCSD--PDAPSKCQRP-CVETCTCKEGFVLSGSECVPATKCGCTYDGRYIPAGQTFWADQNCKQLCKCV 404 HSP 7 Score: 120.939 bits (302), Expect = 8.061e-27 Identity = 180/788 (22.84%), Postives = 287/788 (36.42%), Query Frame = 3 Query: 747 VNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTA--PSDVSAVTWSINIVYENCTSD----TGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGX-----XXXXXXXXXXXXXXVASFGNSFKGFGCP-------DIANTLSTCKPD--DQILANQQCSKIRLK----FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCN-----TCTCNSSLWHCTEKICP-------------------STCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY--------------ITGSCDNTPQSIVDPPAGDNDICSTILN--GPEFSGCMNSSKVDKEFYATYCRRE---QSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC 2903 V TC + G +YRTFD Q + + G C+Y++ D + ++ EN S G V V V + V+S + +N L L + + L I + V+ + +F L+ ++ W + + Y+G GLCGN D+ + + +FG+S+K G D +C ++ ++ C I K F C +++ Y C ++C + LC L Y+++C G I WR C +CP N + C S CP TC + PS CQ V C C G V G C+ C C YDG+ P G D N C + C +K C STC G + TFD K+ F +A ++ + D F ++ N ++ K+V +KV +I+ D LP ++ + + V K + + V K+ F+ + ++ + + GLCG ++GN ++D T K+ + + S+ + SCD T + + D+C I + GP F C +KVD + C + KD L C A+ AC+ G+ TSN P+ + + IC+ C ++C L A C C GC C Y +G QCV S+C C Sbjct: 39 VPQSKMGTCWAMGDPHYRTFDGQRFDFMGTCTYVIAKNCGEDSRLTAFEVHAQNENRGSHRVSYVGLVTVQVYDTTITAVRSETGRVRV----DNSLWSLPIILNNGKLKIFQSGRSVVIEADFGLM------VRYDW---THNLVVTLPDTYSGKTCGLCGNFNGNPKDDFTTPSGTQASGAVAFGSSWKVPGLVADPQCRNDCVGGCGSCTESQLNKWDSDLSCGIITRKENGPFKNCHAIIDPKAYLENCKYDLCMGK----------GLRQFLCNALETYAEACQAAGVQIGDWRGLAKCSPRCPANSKYELCGSACPATCSDP-DAPSK-CQRPCVETCTCKEGFVLSGSECVPATKCGCTYDGRYIPAGQTFWADQNCKQLCKCVAGTRQVACQDKGCGVGQQCKVVDGIRKCQALSYSTCKATGDPHYVTFDNKKYDFQGTCVYQLA---ALCSKDTELTPFEVLVQNEHR------GRNVVSYTKLVQIKVYSLNIL-------------------NDQLVNLPVSLVEGKVSVYK-SGWFAVVTTDFGLKVSFNWHSAAFVTLPSNYMGAVCGLCGNYNGNMQDDLTPKNGNTPVKPTDFGTSWRVAEIPGCIEGCQGVCPSCDITQKVQYE----KEDLCGIIRDTKGP-FRDC--HAKVDPAGFFEDCVYDVCMYEGRKDVL--CQAVTAYTSACQGVGVNVYSWRTSNFCAVKCPI----HSHYDICASACPASCEGL--APPPGCRAHCEEGCSCDEGYILSGD-QCVPFSQCGC 756 HSP 8 Score: 113.62 bits (283), Expect = 1.666e-24 Identity = 172/782 (21.99%), Postives = 292/782 (37.34%), Query Frame = 3 Query: 759 NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNP------LTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLS-----TCKPDDQIL--ANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD---CNTCTC--NSSLWHCTEK-----ICPSTCGIMG--------------TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICST-------------ILNGPE--FSGCMNSSKVDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSR 2918 ++ TC + G +Y TFD + + +QG C Y L N+ EN + V V S G T NP L L + + D + V+ A I + +D + + +++ ++ + G + GLCG D+ + + A G S++ P ++ C + + A+Q C I + F C V+ Y CV ++C + + +C + Y +C +G I WR+ C +CP N ++ CAS CP TC ++ + + S+ C C G + G TC+ C C Y+GQ + G V + C C N ++ EK C G+ G + +FDG +F F C YV + +N D++ F + Q N + G ++ K VTV VN ++ L L + + + E H T F D + V T Y EV V S + + + GLCG ++ ++F +++ KS++ + P+ I D +C I+ P+ F C SK+D Y + C + ++ + C+++ +AC+ G+Q + +S+ A P + +++C+ TC+ TC + C + CF GCEC + G QCV C C + R Sbjct: 1625 SYATCTASGDPHYHTFDGRRFNFQGTCVYQLAGLCSQHPGLVPFNVTVENENRGSKAVSFTK------TVTFSIYGSTLTISKENPYKLNGQLAALPLKSNDELAVFLSGSQAVIET---------AAGITITYDWWSTVRVTVPSNYH-GAMCGLCGNYNGKALDDMTMQNGKTTPDEAKLGESWQVASVPGCSSACQGPWCQACSDSQKKVYQASQFCGIIADETGPFRECHKFVDPAAYVEDCVYDVCQFHGHHGS----------VCKAVEVYLSACQSQGIPIHPWRTQAFCPMECPANSHYNLCASGCPTTCASINSLTTCHMHCSEA--CECDQGHLMSGDTCVPVRDCGCSYNGQYYQKGDVFYPEDTCMEKCICGENGAVSCQKEKCRRGETCKLVNGVKGCHPEEEGKCVASGDPHYTSFDGQRFDFQGT-CVYVLAKVCDDN-DNQLTPFTVTQGNAKYGNGK------VSVTKSVTVTVNNEELNLP-----LSLDKGRLTVTREGHNT---FVRTDFGLKVLYDTVYYVEVIVPSTY------------------QGKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSWVV----DLPEHICGGCEEDCPVCEKANAVLYEKLDSCGIMKAPDGPFQAC--HSKIDPAAYVSDCVFDVCAAGGSKEILCNSVQAYVLACQNAGVQIQPWRSSSFCPASCP----PNSHYEVCADTCRGTCASFIQQLT--CSESCFEGCECDAGFVFDG-AQCVPLENCGCVHDGR 2331 HSP 9 Score: 92.8189 bits (229), Expect = 3.105e-18 Identity = 167/783 (21.33%), Postives = 285/783 (36.40%), Query Frame = 3 Query: 735 GGNDVNNENFT-TCHSYGLDYYRTFDNQLYTYQGRCSYIL--TAPSDVSAVTWSINIVYEN-CTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLT-PLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSF------KGFGCPDI-ANTLSTCKPDDQILANQQ--CSKIRLK----FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFD---CNTCTCNSSLWHCT-----------------EKICP----STCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGD-NDICSTILNGPEFSGCMNSSK---------VDKEFYATYCRREQ-SSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC 2903 GG V +T TC ++G +Y TFD + +QG C YI+ T + V +SI +N + V+ + + D + N + T + L P + + + ++ G+ V + I+ ++ +K ++ V GLCG D + +V +G S+ KG C D TC D++ L Q C + K F C ++ + + CV ++C + +LC L++Y+ C D+G I+ WR + C C + CAS C +C F C + V C+C G V CI ++C C Y G+ + PG D C C+++L T E++C +TC G + TFDG +F F +A S + NV V ++ S ++ K VT + GS + + +P + Q LP D+ L+ ++ ++ + +D ++ + V + GLCG ++G + +D T ++ + +S+ S + P +D + +F G + VD Y C + + S C A+ AC+ GI P QA P+ + + +C+ C +TC +N T C C CEC + +G CV +C C Sbjct: 1223 GGEAVCIPKYTSTCWAWGDPHYHTFDGFDFDFQGTCKYIISKTCGNLNGLVPFSITERNDNRGNAAVSFVREVDVFIYDYNITIRKNQVGQVTVNGELLNLPFQLGDNEVSVFQSGSSAVVETKFGLIVSYDWNWHLVIKLPSSYYS-----------SVCGLCGNFNGDTGDELQNPSGKAVASVIEWGKSWQTPDQDKGHPCWDTCEKNCPTCDDDERELYKTQAFCGGLAAKTNSVFKICNSKLDPQAFMNNCVYDMCLNKGDKR----------MLCQALTSYNKQCRDEGIIIKDWRKEFNCPMNCQRFSHYEHCASPCQPSCP--FPEQKQTCSGACVEACVCDEGYVLSAGVCIPAKTCGCSYQGRYYTPGQQFWADEACSRLCVCDTTLGMVTCREASCSVNEKCIIQDGERVCQPISYATCTASGDPHYHTFDGRRFNFQGTCVYQLAGLCSQHPGLVPFNVTVENENR---------GSKAVSFTKTVTFSIYGSTLTISKENPYKLNGQ----------LAALPLKSNDE--LAVFLSGSQAVIETAAGITITYDWWSTVRVTVPSNYHGAMCGLCGNYNGKALDDMTMQNGKTTPDEAKLGESWQVASVPGCSSACQGPWCQACSDSQKKVYQASQFCGIIADETGPFRECHKFVDPAAYVEDCVYDVCQFHGHHGSVCKAVEVYLSACQSQGI----PIHPWRTQAFCPMECPANSHYNLCASGCPTTCASINSLTT--CHMHCSEACECDQGHLMSGD-TCVPVRDCGC 1954 HSP 10 Score: 86.2705 bits (212), Expect = 3.486e-16 Identity = 132/602 (21.93%), Postives = 234/602 (38.87%), Query Frame = 3 Query: 756 ENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGT----PMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXX-----XXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSIS---DVLCTHLSAYSDSCSDKGSCIQ-WRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQF----DCNT-CTCNSS-LWHCTEKICPST--CGIMGT-----------------QFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQS----NTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY 2435 E C + G +Y +FD Q + +QG C Y+L + C + + + G+ GNG + T S ++VT + L +P + + V R+ + + + G +K+ +D + + + ++ G + GLCGN + ++F NV FG S+ D+ + +D + + + + K C + D F C I + S+ D+ + ++LC + AY +C + G IQ WRS + C CP N + CA C TC + Q + C S C C +G V G C+ E+C C++DG+ TV Q DC + C C +S L C C ST C + G Q +FDG A G AVE + + N F ++ + +S + NE + +G + K SP + T +L + D+++ +++ S ++ + S+ + + V ++L + + G CG ++ SE+D D + + + S+ Sbjct: 2010 EEEGKCVASGDPHYTSFDGQRFDFQGTCVYVLA----------------KVCDDNDNQLTPFTVTQGNA---KYGNGKVSVTKS------VTVTVNNEELNLPLSLDKGRLTVTREGHNTFVRTDFG-LKVLYDTVYYVEVIVPSTYQ-GKMCGLCGNYNKKDRDEFRLPGGKGTKNVNEFGKSW----VVDLPEHICGGCEEDCPVCEKANAVLYEKLDSCGIMKAPDGPFQACHSKIDPAAYVSDCVFDVCAAGGSKEILCNSVQAYVLACQNAGVQIQPWRSSSFCPASCPPNSHYEVCADTCRGTCASFIQQLT--CSESCFEGCECDAGFVFDGAQCVPLENCGCVHDGRYL---TVNQVVVDKDCQSRCVCEASGLVKCQRLSCSSTEVCDVRGGVRSCHIKQAHCTISHAGQLSSFDGMTAAVEAQG----AVEVASLCDETSENWFRVVVDVRVCHKKSAFAPATVHVFFNEATVTVNSQHGIWVNGKKVSPP------------NNLTNQLSVQVLDRSVVIQR----------KSAVRVTYSVSQEVTVTVDNSLASKVCGACGNYNSESEDDLKTADGKVTTDMSVVIGSW 2549 HSP 11 Score: 77.0258 bits (188), Expect = 1.951e-13 Identity = 100/404 (24.75%), Postives = 158/404 (39.11%), Query Frame = 3 Query: 1875 TCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLY--FDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAG------------DNDICSTIL----NGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSY-CSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITI--NC-QTCVCINGRLNCKTKDN 3017 TC MG + TFDG +F F C YV + D R F + N S ++ +VTV+V D + A + G + ++ LP + + + K+ V + + F L +D + ++ + + D GLCG F+GN ++DFT + V S+ PQ D G D+D+ I+ NGP F C + +D + Y C+ + K + C+A+ A AC+ G+Q L C P C A + +++C C +TC + + SKC C C C + +G+ +CV ++C C R IPAG NC Q C C+ G +D Sbjct: 46 TCWAMGDPHYRTFDGQRFDFMGT-CTYVIAKNC--GEDSRLTAFEVHAQNENR------GSHRVSYVGLVTVQV--YDTTITAVRSETGRVRV------DNSLWSLPIILNNGKL---KIFQSGRSVVIEADFGLMVRYDWTHNLVVTLPDTYSGKTCGLCGNFNGNPKDDFTTPSGTQASGAVAFGSSWKVPGLVADPQCRNDCVGGCGSCTESQLNKWDSDLSCGIITRKENGP-FKNC--HAIIDPKAYLENCKYDLCMGKGLRQFLCNALETYAEACQAAGVQIG---DWRGLAKCSPRCPA-NSKYELCGSACPATCSDPD--APSKCQRPCVETCTCKEGFVLSGS-ECVPATKCGCTYDGR----YIPAGQTFWADQNCKQLCKCVAGTRQVACQDK 415
BLAST of SCO-spondin vs. Ensembl Medaka
Match: zanl (zonadhesin [Source:NCBI gene;Acc:101162078]) HSP 1 Score: 221.861 bits (564), Expect = 2.278e-57 Identity = 320/1308 (24.46%), Postives = 507/1308 (38.76%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTW-SINIVYENC--TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSN-----NPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGCPDIAN-TLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLY-DGQ--EHPPGTVRQFDCN-TCTCNSSLWHCTEKICPST------------------CGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG------SCDNTPQSIVDPPAGDN----DICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS-YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREY-APNDVI---------------TIKCAKYICSNRMWNLLSEETANC---EAVCIAYGDPHYKTLDGQMFSFQGSCMYTL-----------MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMH--GTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFY-NGQSYVNGAIIKMENCK-KCQCVQAKFNCTEDYSDC 4424 +TC + G +Y TFD + Y + G CSY+++ + +++ + ++ EN + +K + + V + G++ +N NP +SV + + T +R D + ++ + K + GLCG+ + N +DF D + FG+S+ C N T+ C+P +Q L C + K F C VN + YF C+ ++C ++ +LC + AY + C D+G I WR++T C +CP N + CA C TC PS+ C + C+C G V C+K +SC C + +GQ E P DC C C+S C CP C + G ++TFD ++F C Y N++ F + N E G G S Y+ K + V VN + L + L + + F L L+ Y ++ + ++ +DG+ I V + + + GLCG +DGN+ NDFT+ D + ++ + S+ T S TP DP D C I + C+ VD E + C + +L C + AC+ G + C + + +C +C TC L + C+D C GCEC P + + +CV +C C + S GT +P CP N Y++ C + + S + C C V K CV C C+ + Y P +V+ T++C C +C + CIA GDPHY T D + F G+C Y + + D+ N TIS ++ V+I I + G N +PL P ++ V + +T VS + G+ V +D + + + + +Q + GLCG+Y+G+ ++++ GTM DA +F S+ N D C N P+ C P + E CG++ D NG FA C ++ N Y+ +CL+D C G LC ++ + +C G+ + WR + C + C HY+ CA PC TC S PS C C EGC C G + G+CV +C C + NGQ Y G +E+CK KC+C C SDC Sbjct: 1413 STCIASGDPHYTTFDKRKYNFMGNCSYVMSGICNATSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPVNPTPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKVIKYQDK-------------------LCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGILLDKNSPFAVCHGKVNPNNYFQDCLFDLC----------ELEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPSNSHYEPCADPCQDTCSG---KPSS-CGGACSEGCVCDPGYVLSAGKCVKSDSCGCKHTNGQYYEQPGEEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSIQDCIVSGDPHYNTFDKKFYSFMGT-CTYTLARTCRNDSP----WFSVEGKNEER--GASGVS-YL---KKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSL------------SLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVIKPDKCGKIEDPSGPFECIGV--VDPEPFFQSCVYDMCQFNGELHVLCDQLQAYTDACQSAGATVHHWRAPDFCLDC-----PANSSYSLCVSSCPETC--LGVIGSPGCEDVCVEGCECDPGFILSDD-KCVPLKDCGCVDTS---------GT------------------------YHPPCPPNAEYIECGPACIPTCQEPSTNCSGSCISGCFCKPGFVFK-GPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPSCYPKTSTCIASGDPHYTTFDKHRYDFMGNCSYVMSGICNTTSLPFFEVHADNENRYNSPTISYLK-----AVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYT--VSMTFGVTVRYDGNHFMDIKVI-KYQDKLCGLCGDYNGNSKDDFQKPDGTMT-NDANEFGHSW--NTDPC--------NKQPNATIPGC--EPGEQELYEGYCGILLDKNGPFAVCHGKVNP--NNYFQDCLFDL--CELEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPPNSHYEPCADPCQDTC----SGKPSSCGGA-CSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVA--SDC 2585 HSP 2 Score: 140.198 bits (352), Expect = 1.524e-32 Identity = 131/467 (28.05%), Postives = 189/467 (40.47%), Query Frame = 3 Query: 3033 CPKNQVYMDSVSGCNSCTNYDTCTDTSSI---------ANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFS--------LATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMH--GTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKK 4373 CP N Y D + C TC+D I C C N DG CV C C+ + EY D T+ GDPHY+T DG +QG Y L + ++ P+ + N+ G + + V + + +L G T PL P + I + QL +D GL V +D +R + L ++ +V+GLCGNYDG +NEYM GT+ D +F +S+ + D E P++ E C+ + + CG + D G F++C A+ + Y +C++D C G E C S +AT C G+ + WR Q C + C Y AC SPCP +C + APS C T CVEGC C G + G CVP C C + + Y E+C + Sbjct: 3474 CPPNSHYSDCTAPCPP-----TCSDLFPIFCHLPPTACVEGCEC-NAGYVLSDGKCVSLDKCGCVGSDGEYHDFDRCTVS----------------------------GDPHYRTFDGFTHHYQGPYTYVLTQD-HNLQGSLKPLMVRGKNLRRGGNKRVSFLDEMYVNVYGVDVRFLQKKTVLVNGERVTPPLSPVRGLTITMNSKQMQLTTD-FGLTVRFDGKSRG-VILPRTYSNSVRGLCGNYDGITRNEYMKPDGTV-VRDLNEFGDSWRVSRDT---ETDPDSGF----ETSDCSQSELDDYTSVTKCGALSDPEGPFSACHAALPPQI---YQDCVFDLCA--EDGSEELRCASYEAYATICQEAGVKLGSWRQQLGC-LPCPANSTYTACMSPCPSSCADLA--APSECDATACVEGCQCAAGFVMSEGACVPYPECGCTFLDRYYPLNENFVTEDCSQ 3887 HSP 3 Score: 132.109 bits (331), Expect = 3.650e-30 Identity = 117/388 (30.15%), Postives = 175/388 (45.10%), Query Frame = 3 Query: 3312 AVCIAYGDPHYKTLDGQMFSFQGSCMYTL-----------MDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDI-VIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMH--GTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFY-NGQSYVNGAIIKMENCK-KCQCVQAKFNCTEDYSDC 4424 + CIA GDPHY T D + F G+C Y + + D+ N TIS ++ V+I I + G N +PL P ++ V + +T VS + G+ V +D + + + + +Q + GLCG+Y+G+ ++++ GTM DA +F S+ N D C N P+ C P + E CG++ D NG FA C ++ N Y+ +CL+D C G LC ++ + +C G+ + WR + C + C HY+ CA PC TC S PS C C EGC C G + G+CV +C C + NGQ Y G +E+CK KC+C C SDC Sbjct: 433 STCIASGDPHYTTFDKHRYDFMGNCSYLMSGICNATSLPFFEVHADNENRYNSPTISYLK-----AVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYT--VSMTFGVTVRYDGNHFMDIKVI-KYQDKLCGLCGDYNGNSKDDFQKPDGTMT-NDANEFGHSW--NTDPC--------NKQPNATIPGC--EPGEQELYEGYCGILLDKNGPFAVCHGKVNP--NNYFQDCLFDL--CELEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPPNSHYEPCADPCQDTC----SGKPSSCGGA-CSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPGEEFYVEDCKLKCRCDSPFVTCVA--SDC 787 HSP 4 Score: 122.479 bits (306), Expect = 3.773e-27 Identity = 100/348 (28.74%), Postives = 149/348 (42.82%), Query Frame = 3 Query: 3318 CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN----NDTIPLFPKYDIVIEQLTFFTQLV--SDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAI-QFAESFIANADICPYEISPENNVAPSKEQDM-CNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSY 4334 CI GDPHY T D ++F G+C YTL T N+ P ++ N G S + K + + + T L + N FP + L+ Q V + GLRV WD + + + PS+ + GLCG+YDG+ N++ + F S+ D E+ +P D+ C+ + + CG ++D +G F CI + D ++ +C+YD C N G+ LC L + C G+ V WR + C + C Y C S CP+TC +G C CVEGC C G I + +CVP C C +Y Sbjct: 809 CIVSGDPHYNTFDKNYYTFMGTCTYTLART---CRNDSPWFSVEGKNEERGVSGVSYLKKLYVTVNNITVTLMKSRTTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISV-PSYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEED--------EDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPF-ECIGVV--DPEPFFQSCVYDMCQFN--GELHVLCDQLQAYTDACQSAGVTVHHWRAPDFC-LDCPANSSYSLCVSSCPETC--LGVVGSPGCEDV-CVEGCECDPGFILSDDKCVPLKDCGCVDTSGTY 1135 HSP 5 Score: 121.709 bits (304), Expect = 6.355e-27 Identity = 191/798 (23.93%), Postives = 311/798 (38.97%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTW-SINIVYENC--TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSN-----NPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGCPDIAN-TLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLY-DGQEHPPGTVRQF---DCN-TCTCNSSLWHCTEKICPST------------------CGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG------SCDNTPQSIVDP----PAGDNDICSTI--LNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS-YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINC-QTCVCING 2990 +TC + G +Y TFD Y + G CSY+++ + +++ + ++ EN + +K + + V + G++ +N NP +SV + + T +R D + ++ + K + GLCG+ + N +DF D + FG+S+ C N T+ C+P +Q L C + K F C VN + YF C+ ++C ++ +LC + AY + C D+G I WR++T C +CP N + CA C TC PS+ C + C+C G V C+K ESC C + +GQ + PG +F DC C C+S C CP C + G ++TFD ++F C Y N++ F + N E G G S Y+ K + V VN + L + L + + F L L+ Y ++ + ++ +DG+ I V + + + GLCG +DGN+ NDFT+ D + ++ + S+ T S TP DP D C I L+GP C+ VD E + C + +L C + AC+ G + C + + +C +C TC L + C+D C GCEC P + + +CVS +C C + S P + C Q+C C++G Sbjct: 2231 STCIASGDPHYTTFDKHRYDFMGNCSYVMSGICNTTSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKVIKYQDK-------------------LCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGILLDKNGPFAVCHGKVNPNNYFQDCLFDLC----------ELEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPPNSHYEPCADPCQDTCSG---KPSS-CGGACSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPG--EEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKLQDGELGCYPSSQDCIVSGDPHYNTFDKKYYSFMGT-CTYTLARTCRNDSP----WFSVEGKNEER--GASGVS-YL---KKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSL------------SLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGP--FECIGV--VDPEPFFQSCVYDMCQFNGELHVLCDQLQAYTDACQSAGATVHHWRAPDFCLDC-----PANSSYSLCVSSCPETC--LGVVGSPGCEDVCVEGCECDPGFILS-DDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSG 2956 HSP 6 Score: 120.553 bits (301), Expect = 1.215e-26 Identity = 185/783 (23.63%), Postives = 301/783 (38.44%), Query Frame = 3 Query: 765 TTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTW-SINIVYENC--TSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSN-----NPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVAS-----FGNSFKGFGCPDIAN-TLSTCKPDDQILANQQCSKIRLK---FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLY-DGQEHPPGTVRQF---DCN-TCTCNSSLWHCTEKICPST------------------CGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITG------SCDNTPQSIVDP----PAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLS-YCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPS-----RPKDPEIP 2939 +TC + G +Y TFD Y + G CSY+++ + +++ + ++ EN + +K + + V + G++ +N NP +SV + + T +R D + ++ + K + GLCG+ + N +DF D + FG+S+ C N T+ C+P +Q L C + K F C VN + YF C+ ++C ++ +LC + AY + C D+G I WR++T C +CP N + CA C TC PS+ C + C+C G V C+K ESC C + +GQ + PG +F DC C C+S C CP C + G ++TFD + F C Y N++ F + N E G G S Y+ K + V VN + L + L + + F L L+ Y ++ + ++ +DG+ I V + + + GLCG +DGN+ NDFT+ D + ++ + S+ T S TP DP D C I + C+ VD E + C + +L C + AC+ G+ + C + + +C +C TC L + C+D C GCEC P + + +CV +C C + S RP P P Sbjct: 433 STCIASGDPHYTTFDKHRYDFMGNCSYLMSGICNATSLPFFEVHADNENRYNSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLNPAPEVSVFQSGKHYTVSMTFGVTVRYDGNHFMDIKVIKYQDK-------------------LCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGILLDKNGPFAVCHGKVNPNNYFQDCLFDLC----------ELEGAKPILCEAIEAYVNECQDRGVDIGPWRNETFC-LKCPPNSHYEPCADPCQDTCSG---KPSS-CGGACSEGCVCDPGYVLSAGKCVKRESCGCKHTNGQYYEPG--EEFYVEDCKLKCRCDSPFVTCVASDCPPMHECKMQDGKLGCYPSFQDCIVSGDPHYNTFDKNYYTFMGT-CTYTLARTCRNDSP----WFSVEGKNEER--GVSGVS-YL---KKLYVTVNNITVTLMKSRTTLVNGKRVAFPHNPSPLMSL------------SLSGQYVILQTTFGLRVRWDGNHHAQISVP-SYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPFECIGV--VDPEPFFQSCVYDMCQFNGELHVLCDQLQAYTDACQSAGVTVHHWRAPDFCLDC-----PANSSYSLCVSSCPETC--LGVVGSPGCEDVCVEGCECDPGFILS-DDKCVPLKDCGCVDTSGTYHPRPSTPNPP 1145 HSP 7 Score: 118.627 bits (296), Expect = 5.075e-26 Identity = 104/371 (28.03%), Postives = 158/371 (42.59%), Query Frame = 3 Query: 3318 CIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGN----NDTIPLFPKYDIVIEQLTFFTQLV--SDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAI-QFAESFIANADICPYEISPENNVAPSKEQDM-CNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPV-KWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAI---IKMENCKK-CQCVQA 4391 CI GDPHY T D + +SF G+C YTL T N+ P ++ N G S + K + + + T L + N FP + L+ Q V + GLRV WD + + + PS+ + GLCG+YDG+ N++ + F S+ D E+ +P D+ C+ + + CG ++D +G F CI + D ++ +C+YD C N G+ LC L + C G V WR + C + C Y C S CP+TC +G C CVEGC C G I + +CV C C +Y + +E C++ C+C+ Sbjct: 2607 CIVSGDPHYNTFDKKYYSFMGTCTYTLART---CRNDSPWFSVEGKNEERGASGVSYLKKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFGLRVRWDGNHHAQISV-PSYHDQMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEED--------EDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGPF-ECIGVV--DPEPFFQSCVYDMCQFN--GELHVLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCPANSSYSLCVSSCPETC--LGVVGSPGCEDV-CVEGCECDPGFILSDDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSG 2956 HSP 8 Score: 96.6709 bits (239), Expect = 2.324e-19 Identity = 130/571 (22.77%), Postives = 224/571 (39.23%), Query Frame = 3 Query: 1260 VASFGNSFKGFGCPD------IANTLSTCKPDDQILANQQCSKIRLK--FPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCL-YDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKL--YFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY-ITGSCDNTPQS---IVDPPAGDNDICSTI-----LNGPE--FSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCD-DDCFPGCECPPNYYKTGTGQCVSESECTC 2903 V GNS+K G D + C +++ QC ++ L F C +V+ D + C+ ++C + + LC ++ AY+ +C G I WR+ T C CP N +SDC + CP TC +LF + V C C +G V C+ + C C+ DG+ H D + CT + + TFDG + Y V +N ++ N G ++++E + V V G D+ + V ++GE T P + +T ++++++ F L FDG KS + + N++ GLCG +DG + N++ + D ++ + S+ ++ + P S D + D +++ L+ PE FS C + + + Y + + + C++ A C+ G++ + C+P C A T + C C S+C +L A S+CD C GC+C + + G CV EC C Sbjct: 3366 VIELGNSWKSEGDSDPCQPDNRPDVHPNCTAEEEEQFKAQCEQVILSSSFRPCHSVVSPDAFLGNCIYDMCEYN----------GMQATLCDNVEAYAQACQSAGVTITWRNSTFC-LPCPPNSHYSDCTAPCPPTCSDLFPIFCHLPPTACVEGCECNAGYVLSDGKCVSLDKCGCVGSDGEYH--------DFDRCTVSGD-----------------PHYRTFDGFTHHYQG---PYTYVLTQDHNLQGSLKPLMVRGKNLRR--GGNKRVSFLDE---MYVNVYGVDVRFL--------QKKTVLVNGER------VTPPLSPVRGLTITMNSKQMQLTTDFGLTVRFDG-KSRGVILPRTYSNSVRGLCGNYDGITRNEYMKPDGTVVRDLNEFGDSWRVSRDTETDPDSGFETSDCSQSELDDYTSVTKCGALSDPEGPFSAC--HAALPPQIYQDCVFDLCAEDGSEELRCASYEAYATICQEAGVKLG---SWRQQLGCLP-CPANST-YTACMSPCPSSCADL--AAPSECDATACVEGCQCAAGFVMS-EGACVPYPECGC 3867 HSP 9 Score: 83.5741 bits (205), Expect = 2.367e-15 Identity = 85/351 (24.22%), Postives = 142/351 (40.46%), Query Frame = 3 Query: 3162 DGTCVQPSDCPCIYMNREYAPNDVIT-------------IKCAKYICSNRMWNLLSE---------ETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGF-SLATCAKFVQIIIGGQTYLLSRGN----NDTIPLFPKYDIVIEQLTFFTQLV--SDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLC 4127 D CV DC C+ + Y P V + + C N N ++E E + +C GDPHY T D + + G+C YTL +SS+++P T+ N G + + + V I + G T + +G N T+ + P I ++ F + V S GLRV +D + + L S+ G + G+C D + ++ + +S+ + P N Q+ C+ + ++ CG++ D N +F C + + Y+ NC+YD C GG LC +L ++A CA G+ + WR C Sbjct: 2915 DDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSGGQIQCHNTSCNPVTEVCQLQDGLYECSPLNGICSVSGDPHYTTFDKRTHHYMGACSYTLTKL-CNSSSDLPHFTVDTQNEHRGSNTKVSYVRAVVINVYGMTVVFGKGRSVQVNGTVVVPPVSSISGVKIYFSGKFVVLEASFGLRVRFDGNHHADVTLPSSYSGLLCGMCNFNDNPTDDNLKPDNTPAANSNELGDSWAVP------DPRPCTN---GGGQEECDEKVEEEAQKPTSCGMITDPN-IFKPCHAVVPPE--PYFENCVYDMCA--TGGQTVALCQALESYADICAAAGVNIPWRNSTFC 3250 HSP 10 Score: 80.4925 bits (197), Expect = 1.706e-14 Identity = 125/471 (26.54%), Postives = 184/471 (39.07%), Query Frame = 3 Query: 1593 CPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCL-YDGQEHPPGTVRQFD--CNTCTC-NSSLWHCTEKIC------------------PSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDI-ILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDP-PAGDNDI----CSTIL--NGPEFSGCMNSSKVDKEFYATYCRR---EQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC 2903 CP N + +C C TC Q PS C S + C C G V G C+ E C CL DG + PG V D C C + CT+ C STC G + TFD ++ F C Y+ + N T + F + ++ E+ NS I+ K V V V+ I ILK Q+ + N+ ++ P + V + YT V ++ + +DG+ + IKV ++ L GLCG ++GNS++DF + D ++ S+ T C+ P + + G+ ++ C +L NGP F+ C KV+ Y C E K L C AI C+ G+ C P + ++ C+ CQ TC S C C GC C P Y + G+CV C C Sbjct: 322 CPPNAEYIECGPACIPTC----QEPSTNCSGSCISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCVGNYTLECTDNSCDPSEECREVNGVPGCYPKTSTCIASGDPHYTTFDKHRYDFMG-NCSYL-MSGICNAT---SLPFFEVHADNENRY----NSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPLN------------PAPEVSVFQSGKHYT-VSMTFGVTVRYDGNHFMDIKVI-KYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGILLDKNGP-FAVC--HGKVNPNNYFQDCLFDLCELEGAKPIL--CEAIEAYVNECQDRGVDIGPWRNETFCLKCPP-----NSHYEPCADPCQDTC----SGKPSSCGGACSEGCVCDPGYVLS-AGKCVKRESCGC 750 HSP 11 Score: 80.1073 bits (196), Expect = 2.140e-14 Identity = 63/242 (26.03%), Postives = 106/242 (43.80%), Query Frame = 3 Query: 3612 LVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMK-YEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSY 4334 L+ + GL+V +D + + + + + G+CGNY+ D ++ + + +D I+ S+ + D P + +V P+ C A + + A+ ++ + F C + D + NC+YD C N G LC ++ +A C G+ + WR C + C HY C +PCP TC + H T CVEGC C G + +G+CV C C + Y Sbjct: 3311 LLDTNFGLQVKFDGVHHLEITVPGEYFNKLCGMCGNYNHDSTDDNLKPDKQPAKDVIELGNSWKSEGDSDPCQPDNRPDVHPN-----CTAEEEEQFKAQCEQVILSSS---FRPCHSVVSPDA--FLGNCIYDMCEYN--GMQATLCDNVEAYAQACQSAGVTITWRNSTFC-LPCPPNSHYSDCTAPCPPTCSDLFPIF-CHLPPTACVEGCECNAGYVLSDGKCVSLDKCGCVGSDGEY 3538 HSP 12 Score: 75.485 bits (184), Expect = 6.653e-13 Identity = 130/602 (21.59%), Postives = 234/602 (38.87%), Query Frame = 3 Query: 783 GLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIV-YENCTSDTGCVKVIVIEAGDVVV------KSSGNGILYTTDSN-NPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLST----CKP--DDQILANQQCSKIR-LKFP-GCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVR------------------QFDCNTCTCN---------SSLWHCTEKICPSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIIL-KATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSY 2435 G +Y TFD + Y++ G C+Y L + +S+ E S +K + + D+ V ++ NG N +PL LS++ YV+ Q F ++++WD N++ IS+ + + GLCG ++ D + NV FGNS++ D + +T C P + ++ +C KI L P C +V+ + +F CV ++C + + VLC L AY+D+C G+ + WR+ C CP N +S C S CP TC + +P C++ V C C G + C+ + C C+ + P V Q C+ +CN L+ C+ C + G + TFD + C Y + N++ D + V Q+ G++ ++ + V + V G ++ K S Q+ + T +P + + + + ++ S ++ FDG+ + + + L G+C F+ N +D + DN+ + + S+ Sbjct: 2611 GDPHYNTFDKKYYSFMGTCTYTLARTCRNDSPWFSVEGKNEERGASGVSYLKKLYVTVNDITVTLMKSKRTLVNGKRVAFPHNPSPLMSLSLSG-----------QYVILQTTF--------GLRVRWDGNHHAQISVPSYHD--QMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDLSGPFECIGVVDPEPFFQSCVYDMCQFNGELH----------VLCDQLQAYTDACQSAGATVHHWRAPDFC-LDCPANSSYSLCVSSCPETCLGVVGSPG--CEDVCVEGCECDPGFILSDDKCVSLKDCGCVDTSGTYHPFQVDDEWLLEGCEQSCECLSGGQIQCHNTSCNPVTEVCQLQDGLYECSPLN--GICSVSGDPHYTTFDKRTHHYMGA-CSYTLTK-LCNSSSDLPHFTVDTQNE------HRGSNTKVSYVRAVVINVYGMTVVFGKGRSVQV------------NGTVVVPPVSSISGVKIY-FSGKFVVLEASFGLRVRFDGNHHADVTLPSSYSGLLCGMC-NFNDNPTDDNLKPDNTPAANSNELGDSW 3154 HSP 13 Score: 73.559 bits (179), Expect = 2.269e-12 Identity = 123/468 (26.28%), Postives = 180/468 (38.46%), Query Frame = 3 Query: 1602 NKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCL-YDGQEHPPGTVRQFD--CNTCTCNSSL-WHCTEKIC------------------PSTCGIMG-TQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDI-ILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDP-PAGDNDI----CSTIL--NGPEFSGCMNSSKVDKEFYATYCRR---EQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTC 2903 N + +C C TC Q PS C S + C C G V G C+ E C CL DG + PG V D C C + CT+ C STC G + TFD K+ F C YV + N T + F + ++ E+ NS I+ K V V V+ I ILK Q+ + N+ ++ P + V + YT V ++ + +DG+ + IKV ++ L GLCG ++GNS++DF + D ++ S+ T C+ P + + G+ ++ C +L N P F+ C KV+ Y C E K L C AI C+ G+ C + ++ C+ CQ TC S C C GC C P Y + G+CV C C Sbjct: 1305 NAEYIECGPACIPTC----QEPSTNCSGSCISGCFCKPGFVFKGPRCVPIEKCGCLDEDGNYYEPGEVVLGDGCSKLCRCAGNYTLECTDNSCDPSEECREVNGIPGCYPKTSTCIASGDPHYTTFDKRKYNFMG-NCSYV-MSGICNAT---SLPFFEVHADNENRY----NSPTISYLKAVHVYVHMVKISILKGGVVQVNGTNVNLPVN------------PTPEVSVFQSGKHYT-VSMTFGVTVRYDGNHFMDIKVI-KYQDKLCGLCGDYNGNSKDDFQKPDGTMTNDANEFGHSWNTDPCNKQPNATIPGCEPGEQELYEGYCGILLDKNSP-FAVC--HGKVNPNNYFQDCLFDLCELEGAKPIL--CEAIEAYVNECQDRGVDIGPWRNETFCLKC-----PSNSHYEPCADPCQDTC----SGKPSSCGGACSEGCVCDPGYVLS-AGKCVKSDSCGC 1730 HSP 14 Score: 67.781 bits (164), Expect = 1.197e-10 Identity = 91/365 (24.93%), Postives = 150/365 (41.10%), Query Frame = 3 Query: 759 NFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNT---DSALIIPGTP---MYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCG-----XXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLST----CKP--DDQILANQQCSKIRLKFPG--CADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCI-QWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCL-YDGQEHP 1790 +F C G +Y TFD YT+ G C+Y L + +S+ E + G V ++ LY T +N +T + T + P P M + +++L G ++++WD N++ IS+ + + GLCG ++ D + NV FGNS++ D + +T C P + ++ +C KI C +V+ + +F CV ++C + + VLC L AY+D+C G + WR+ C CP N +S C S CP TC + +P C++ V C C G + C+ + C C+ G HP Sbjct: 805 SFQDCIVSGDPHYNTFDKNYYTFMGTCTYTLARTCRNDSPWFSV----EGKNEERGVSGVSYLKK------------LYVTVNNITVTLMKSRTTLVNGKRVAFPHNPSPLMSLSLSGQYVILQTTFG-LRVRWDGNHHAQISVPSYHD--QMCGLCGDYDGNAGNDFTKPDGTPAGNVNDFGNSWQTEEDEDECSPGTTPDLDCDPELEAEVTKPDKCGKIEDPSGPFECIGVVDPEPFFQSCVYDMCQFNGELH----------VLCDQLQAYTDACQSAGVTVHHWRAPDFC-LDCPANSSYSLCVSSCPETCLGVVGSPG--CEDVCVEGCECDPGFILSDDKCVPLKDCGCVDTSGTYHP 1137
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 3425.18 bits (8880), Expect = 0.000e+0 Identity = 1676/1676 (100.00%), Postives = 1676/1676 (100.00%), Query Frame = 3 Query: 12 MGTKTHTIWQMNFNQXXXXXXXXXXXTSQSVESDKVLIGVKPKSVTSPNNCIYDYNEDVDTVTESCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYVSSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 MGTKTHTIWQMNFNQLLLSILLYAILTSQSVESDKVLIGVKPKSVTSPNNCIYDYNEDVDTVTESCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYVSSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF Sbjct: 1 MGTKTHTIWQMNFNQLLLSILLYAILTSQSVESDKVLIGVKPKSVTSPNNCIYDYNEDVDTVTESCNKYIGLMMENYNLDEKSVPIAEQYQDPSIPVSKQQSLSMQLYVSSGMPEPQCNFTLQNIVSEENCCVGYENFPYCNISVCYSDNGGCYNGGICMSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1676
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 3129.35 bits (8112), Expect = 0.000e+0 Identity = 1517/1517 (100.00%), Postives = 1517/1517 (100.00%), Query Frame = 3 Query: 489 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF Sbjct: 1 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 3128.19 bits (8109), Expect = 0.000e+0 Identity = 1517/1517 (100.00%), Postives = 1517/1517 (100.00%), Query Frame = 3 Query: 489 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF Sbjct: 1 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 3127.81 bits (8108), Expect = 0.000e+0 Identity = 1517/1517 (100.00%), Postives = 1517/1517 (100.00%), Query Frame = 3 Query: 489 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF Sbjct: 1 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 3127.42 bits (8107), Expect = 0.000e+0 Identity = 1517/1517 (100.00%), Postives = 1517/1517 (100.00%), Query Frame = 3 Query: 489 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDXXXXXXXXXXXXXXXGDVTGLCGXXXXXXXXXXXXXXXVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKXXXXXXXXXXXXEHGCECICKEPEMILCXXXXXXXXXXXNCQCINGTKQCDGVYDCWNGFDEKNCEIF 5039 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF Sbjct: 1 MSPNQCKCQENYAGMQCDEDNPIIIQSQHPSQLYCYQSDQCFAFKKSDFLGKAVSYDECCADKTGSWGSQSGSCVSCKANFAGGNDVNNENFTTCHSYGLDYYRTFDNQLYTYQGRCSYILTAPSDVSAVTWSINIVYENCTSDTGCVKVIVIEAGDVVVKSSGNGILYTTDSNNPLTPLSVTNTDSALIIPGTPMYVLRQDNFILLSLEAGNIKLKWDNNNNIFISIHNSIYNGDVTGLCGNADNNPSNDFSNFDNVASFGNSFKGFGCPDIANTLSTCKPDDQILANQQCSKIRLKFPGCADLVNSDTYFSMCVQNICTKSQNSNTTLDIPSISDVLCTHLSAYSDSCSDKGSCIQWRSDTLCETQCPGNKIWSDCASECPVTCRNLFQTPSAYCQNSKVGRCICGSGEVEYGYTCIKPESCPCLYDGQEHPPGTVRQFDCNTCTCNSSLWHCTEKICPSTCGIMGTQFDTFDGAKFAFNDVGCQYVAVEPSVNNTDDRANVFVIIQSNTESMLGTEGNSAYINEPKIVTVKVNGSDIILKATSPQLGDSQTNVFIDGEDHTTKLPFTIPDKNIHVRKLTNIYTEVKVSSHFKLYFDGSKSIYIKVSDALKNNLIGLCGKFDGNSENDFTEKDNSIRESIVNIAKSYITGSCDNTPQSIVDPPAGDNDICSTILNGPEFSGCMNSSKVDKEFYATYCRREQSSNKDQLSYCSAILNAAIACRRFGIQYEVPFTSNILQACIPLCQAGGTVFQICSRTCQSTCRELNEATASKCDDDCFPGCECPPNYYKTGTGQCVSESECTCWNPSRPKDPEIPAGTNITINCQTCVCINGRLNCKTKDNCTNPVCPKNQVYMDSVSGCNSCTNYDTCTDTSSIANSCGCPNDTVSKIDGTCVQPSDCPCIYMNREYAPNDVITIKCAKYICSNRMWNLLSEETANCEAVCIAYGDPHYKTLDGQMFSFQGSCMYTLMDTPGDSSNNIPPITISVANIACGFSLATCAKFVQIIIGGQTYLLSRGNNDTIPLFPKYDIVIEQLTFFTQLVSDSIGLRVLWDRSTRVYLFLKPSFQGNVQGLCGNYDGDLQNEYMHGTMKYEDAIQFAESFIANADICPYEISPENNVAPSKEQDMCNANPDRKSWAEQMCGVMKDTNGVFASCIEAMHQDVNRYYSNCLYDACGCNRGGDCECLCTSLATFATQCAFLGIPVKWRTQNLCPIMCEGGKHYDACASPCPQTCDTIGSNAPSHCSTTHCVEGCSCPNGMIDLNGECVPPTTCPCFYNGQSYVNGAIIKMENCKKCQCVQAKFNCTEDYSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNSDEISCGCLPHQIVCNISRTCIEEKKICDGNYDCGVNDKSDEENCKKCQMTEYKCNNSQICILLNNKCDGQDDCGDGSDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517 The following BLAST results are available for this feature:
BLAST of SCO-spondin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SCO-spondin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SCO-spondin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SCO-spondin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SCO-spondin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SCO-spondin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008350 ID=SMED30008350|Name=SCO-spondin|organism=Schmidtea mediterranea sexual|type=transcript|length=5142bpback to top protein sequence of SMED30008350-orf-1 >SMED30008350-orf-1 ID=SMED30008350-orf-1|Name=SMED30008350-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1713bp IIEMGTKTHTIWQMNFNQLLLSILLYAILTSQSVESDKVLIGVKPKSVTSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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