SMED30008321

Overview
NameSMED30008321
Smed IDSMED30008321
Length (bp)3250
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008321 (SMED30008321) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008321 (SMED30008321) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008321 (SMED30008321) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008321 (SMED30008321) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008321

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30008321h1SMcG0011114 SmedASXL_017458SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30008321 vs. Planmine SMEST
Match: SMESG000068172.1 (SMESG000068172.1)

HSP 1 Score: 1946.01 bits (5040), Expect = 0.000e+0
Identity = 982/986 (99.59%), Postives = 984/986 (99.80%), Query Frame = 3
Query:   78 MRSIFNXXXXXXXXXXXXKFSKTTLSLDIKPTKNVLKLCDHGNSSCVNELTQRFVIFNSEINQNITVKLGDLQSALPLNEKSESYNVKIFAERRGYLPPSNSNEIVINTICQFTYSLAIMSRLELDFCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYSQYLNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQKSTILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLKITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKIIPRNNKHKARLYDDATEVKIPLRTSNDLIILPVVKESRDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNPWSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQLKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNTHPLSSTLYTTISKHKTKSSLINAVAISSINLVHISIYICTGFVACIFIGCVLFKLICYRNGHESTVEDGDGSTNYQRSASGFLEDPNLKVQVRSSNNKNFSALSKCSTNCLRQNTNKTHNNEXXXXXXXXXXXIATNYDLTLSEPICNESAVDSGIELGKLSSYSSNTCPSNDLCCTIDLYPSCSGTDDSLMHQPYQSGHFVNPMIGFYPQKGLHESEIQYQESMMLSSMNSTKNISNLRHPYW 3035
            MRSIFNLIISLTILVIIIKFSKTTLSLDIKPTKNVLKLCDHGNSSCVNELTQRFVIFNSEINQNITVKLGDLQSALPLNEKSESYNVKIFAERRGYLPPSNSNEIVINTICQFTYSLAIMSRLELDFCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYSQYLNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQKSTILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLKITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKIIPRNNKHKARLYDDATEVKIPLRTSNDLIILPVVKESRDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNPWSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQ+KFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNT PLSSTLYTTISKHKTKSSLIN VAISSINLVHISIYICTGFVACIFIGCVLFKLICYRNGHESTVEDGDGSTNYQRSASGFLEDPNLKVQVRSSNNKNFSALSKCSTNCLRQNTNKTHNNEPYPPLLPPPGPIATNYDLTLSEP+CNESAVDSGIELGKLSSYSSNTCPSNDLCCTIDLYPSCSGTDDSLMHQPYQSGHFVNPMIGFYPQKGLHESEIQYQESMMLSSMNSTKNISNLRHPYW
Sbjct:    1 MRSIFNLIISLTILVIIIKFSKTTLSLDIKPTKNVLKLCDHGNSSCVNELTQRFVIFNSEINQNITVKLGDLQSALPLNEKSESYNVKIFAERRGYLPPSNSNEIVINTICQFTYSLAIMSRLELDFCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYSQYLNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQKSTILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLKITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKIIPRNNKHKARLYDDATEVKIPLRTSNDLIILPVVKESRDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNPWSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQIKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNTQPLSSTLYTTISKHKTKSSLINEVAISSINLVHISIYICTGFVACIFIGCVLFKLICYRNGHESTVEDGDGSTNYQRSASGFLEDPNLKVQVRSSNNKNFSALSKCSTNCLRQNTNKTHNNEPYPPLLPPPGPIATNYDLTLSEPVCNESAVDSGIELGKLSSYSSNTCPSNDLCCTIDLYPSCSGTDDSLMHQPYQSGHFVNPMIGFYPQKGLHESEIQYQESMMLSSMNSTKNISNLRHPYW 986          
BLAST of SMED30008321 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 128.257 bits (321), Expect = 1.317e-29
Identity = 185/758 (24.41%), Postives = 326/758 (43.01%), Query Frame = 3
Query:  231 QRFVIFNSEI-NQNITVKLGDLQSALPLNEKSES-YNVKIFAERRGYLPPSNSNEIVINTICQFTYSLAIMSRLEL--DFCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYS-----QYLNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQK--STILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLK-------------ITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKII--PRNNKHKARLYDDATEVKIPLRTSND-LIILPVVKESRDIFSIYSKPN--IDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVD------PIHLSVHSNNQSLMSMDM---NYQTYL--NPWSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEE----CVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQLKFYPED-IFSIEKSLNQEYFLITAKQADKSS-SIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVS-LTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPD--SDMFI--------YKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNTHPLSSTLYTT 2333
            + F   N  + N N+T KL D    +  N+  +S ++V I  ++  Y     +    INTIC+F Y   I  ++ L  + CCR+ +  ++ + +       C  SL R    CFI + F  S        +     L       +S  C    +   I  VSK   +SFS+ L V W          ++ IG P YS+     +  P++L+SD   + +Q+  + C +  N    SV  + EN+   +       N  I+ + L+             +   L   +M+  +T       KN L+     C+II      + K+  Y       + +  + D  II+P++K +R  FSI++  N  I  ++D + LY I +  D S   +++      +D      P+ +   S+       D    N +T +  N + + ++       L +P+ +W  + WKL ++ +++  I  W V    S      CV  ++ +RLK++ R+      GEK + G+    +N  P++D+T+    N ++F P   I S ++  ++   LI   Q+D +   ++L S  S+  L  +++      + + ++   C+ +L L Q K      +  S Y+ + V  +      K CYL + VK SD S  RL  I    Y+  +T  D  S  +I        Y +  NQL         N+SLYE  SC   +LIF+  +  + ++ S +I P T  +SST+ +T
Sbjct:   45 KSFAFINPAVHNFNLTTKLED---DIKDNQFIKSDFSVSIHTDKHQYYDLDKTESYTINTICKFDY---IPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMFQKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISD--KMFSQM-NLQCIISENLEIKSVFFD-ENIFDAQYKIAALPNRFIITILLENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQTVHIAINTDKFIIIPILK-TRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKVINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGST--SSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSE---LIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKS-----DIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQL---------NISLYESGSCRFSKLIFTGLVR-INIQHSEDI-PTT--ISSTMIST 768          
BLAST of SMED30008321 vs. Planmine SMEST
Match: SMESG000036221.1 (SMESG000036221.1)

HSP 1 Score: 105.145 bits (261), Expect = 1.363e-22
Identity = 170/744 (22.85%), Postives = 315/744 (42.34%), Query Frame = 3
Query:  255 EINQNITVKLGDL---QSALPLNEKSESYNVKIFAERRGYLPPSN--SNEIVINTICQFTYSLAIMSR-LELD-FCCRVELLIYSKSKSILNPFGVCDLSLRRDEVHCFIPIKFVYSQY----LNEKILPLIGIVHKKSSGSCHFQPEPKDIYKVSKGLGISFSSRLPVIWQPTHSQKS--TILIGIPLYSVKDYENLLIPVRLVSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIVDVKLKITPSLDQQLMFLNVTVK---LDSMTKNSLKFIKVKCKIIPRN---NKHKARLYDDATEVKIPLRTS-NDLIILPVVKES--RDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNP-WSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQLKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTN-------PDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIE-WVTVEPSNNIVPNTHPLSSTLYTTISKHKTKSSLINAVAISSIN 2393
            E   NI+V  G+L   ++  PLN     Y   +F ++  +   SN  ++   +  ICQFT+     SR  E +  CC + L     +  I +    C + + +D   C +  +  +  +      E+ L +   V     G C           +     ++    L V W+   S  S   +LIG+PL S++     + P+++ S  V      + I C    +    S+  +S   +       + +   +++    +  S  Q+++ L V ++   L+S+ K S  +I   CKII  +   NK K        E KI ++ +     I P++K S    I      P I+   + L++    N  D S   I+   +  PV P  L      ++  S +++ +    P W++ +       T+  P+ +W+ + W+L  +D  L  I  W  Y+  + +C P ++V R K++ RL       ++ Y G  + D  +IP +D+T   +   +K    + F+++  L  ++ L+ + Q   SS I +F  +S + +    I AD Q +S+VD+ V+CI  + + Q   S    N   ++K +     S+  ++ CY  + + F+D     L     T +QL L +        D + FIY  +       +   +    L E   C   RLIF K +  +   + +N I P+   L S + T      T++S I++++  S+N
Sbjct:   50 ENKMNISVLFGNLTKTKNLEPLN-----YITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHG---NSRPFEYNKVCCEMILKRLGTNSKIFDR-KYCQMDVNKDTSVCSVQFQIPHENFEFKTSTEEQLDISSRVFASQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAV---YNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKITDTKKEILIEESNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWIS--CKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGP-IEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKT--SNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWN-YKNSNYQCEPVFQVTRCKLLARLMKDTFDSKRSYYGQSELD--KIPLLDMTPYLRKQNIKLSTNN-FNVD--LRNDFMLLQSVQIG-SSEISIFDNHS-SKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINS----NDLPFHKILVSQRLSEDSTQACYFQILLTFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQ-----YKEPGKFIFQLLESTEC-LFRLIFQKDVNIYENTKSTNPIDPS---LQSPIITKSQSTSTETSKISSLSTKSLN 755          
BLAST of SMED30008321 vs. Planmine SMEST
Match: SMESG000016285.1 (SMESG000016285.1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.287e-17
Identity = 95/362 (26.24%), Postives = 162/362 (44.75%), Query Frame = 3
Query: 1410 YLNPWSIQVQFIDNLHTLISPV-RVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQL-KFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISA-DQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKI----EWVTVEPSNNIVPNT-HPLSSTLYTTISKHKTKSSLINAVAISSINLVHISIYICTGFVACIFIGCVLFKLI 2471
            + + W + V+ I++  T++  +  VW+PESWKL LSD  L PI +W VY+  ++ CV  Y+ A LKI  RL      G + Y+G    D    P++DV+  T ++ L    P ++ S  ++ N+   L+  K +++ + I L    +   L   +IS     + SVV     C+  L L +   S+   N    + +  + +   +  + C L + V F+D S   L  +    Y+L +          +  +  LV  R   R+N+ LY    C+    + +  I    +W+    ++  +P T  P+ +T   T S    KSS      I   N   I++Y   G VA   +  +L  LI
Sbjct:  425 FTDSWVLPVE-IEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYR--NKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAK--PWVDVSKETDISSLITTKPSEVVSFSRN-NRGSILVDVK-SNQRTVISLVDRRNNL-LADTEISVFSDHWASVVRFYGRCLSQLDLHE---SQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDG-------MEFRNGDLVNFRL--RINIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSS---ETFIEPRNSFSIAVYCIVGIVAMAVVILLLNCLI 763          
BLAST of SMED30008321 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.380e-10
Identity = 72/263 (27.38%), Postives = 125/263 (47.53%), Query Frame = 3
Query: 1584 YKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQLKFYPED-IFSIEKSLNQEYFLITAKQADKSS-SIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVS-LTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPD--SDMFI--------YKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNTHPLSSTLYTT 2333
            ++ +RLK++ R+      GEK + G+    +N  P++D+T+    N ++F P   I S ++  ++   LI   Q+D +   ++L S  S+  L  +++      + + ++   C+ +L L Q K      +  S Y+ + V  +      K CYL + VK SD S  RL  I    Y+  +T  D  S  +I        Y +  NQL         N+SLYE  SC   +LIF+  +  + ++ S +I P T  +SST+ +T
Sbjct:    2 FQRSRLKVLVRMVNRNTLGEKEFYGSTS--SNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSE---LIVDPQSDVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKS-----DIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQL---------NISLYESGSCRFSKLIFTGLVR-INIQHSEDI-PTT--ISSTMIST 241          
The following BLAST results are available for this feature:
BLAST of SMED30008321 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008321 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068172.10.000e+099.59SMESG000068172.1[more]
SMESG000014253.11.317e-2924.41SMESG000014253.1[more]
SMESG000036221.11.363e-2222.85SMESG000036221.1[more]
SMESG000016285.11.287e-1726.24SMESG000016285.1[more]
SMESG000014253.13.380e-1027.38SMESG000014253.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008321 ID=SMED30008321|Name=SMED30008321|organism=Schmidtea mediterranea sexual|type=transcript|length=3250bp
ATTTAAATTATAAATTTAATTTTATCATAAATTTATATTTTATATAATAA
AAATTAATAATTTCAAGCAATCTAAGAATGCGTTCGATTTTTAATTTAAT
TATATCCCTGACGATTTTAGTAATTATTATTAAATTCTCCAAAACGACAT
TGTCACTTGATATCAAGCCGACTAAGAATGTATTGAAGTTGTGTGATCAT
GGAAACAGTTCCTGTGTCAATGAATTGACCCAGAGATTTGTGATTTTCAA
CTCAGAAATCAATCAAAATATCACAGTGAAACTAGGTGATTTGCAGAGCG
CTTTACCGTTGAATGAAAAATCCGAATCGTATAACGTGAAGATCTTTGCC
GAACGTCGTGGATATTTACCGCCATCAAATTCAAATGAGATCGTAATTAA
TACAATTTGTCAATTTACTTATTCGCTAGCTATCATGTCTCGCCTGGAAC
TCGATTTCTGTTGCCGGGTCGAATTATTGATCTACAGTAAATCGAAATCG
ATTCTAAATCCGTTTGGCGTTTGTGATTTGAGCCTGAGACGCGATGAGGT
CCATTGTTTTATTCCAATTAAGTTTGTCTATTCTCAATATCTTAACGAAA
AAATTCTGCCTCTTATTGGAATTGTCCACAAGAAAAGTTCTGGCAGTTGC
CATTTTCAACCGGAACCCAAAGACATTTATAAAGTGTCCAAGGGCCTCGG
GATTTCATTCAGTAGCCGGTTGCCGGTCATTTGGCAACCGACACACTCAC
AGAAATCCACAATTCTAATAGGAATTCCTCTCTATTCGGTGAAAGATTAT
GAAAATCTGTTGATTCCAGTTCGACTTGTGTCGGATCTCGTGGATTTAAC
CAATCAAATCATTCGAATTAATTGTCAACTCGATCCGAATTTTGTGTTTC
ATTCAGTCATTGTGAATTCTGAAAACGTGTCTATAATAGAGGATCCAGAG
ATCGAAGATGAAAATTGGACTATCGTCGATGTGAAATTGAAAATTACTCC
CAGTTTGGATCAGCAATTGATGTTTCTCAATGTCACAGTTAAGTTGGACT
CGATGACGAAAAATTCTCTGAAATTCATTAAAGTAAAATGTAAAATCATT
CCGAGAAATAACAAGCATAAAGCTCGTTTATATGATGACGCAACTGAAGT
GAAAATTCCATTACGTACATCGAATGATTTAATAATACTACCAGTTGTTA
AGGAATCAAGGGATATTTTCTCGATATATTCAAAGCCAAATATTGACTTC
CAAAATGATCCATTACAATTATATATCATAACAAACTATAGAGATAGATC
AGTGAAAAAGATTTCATCCAAATTTCAAATGTATCCAGTTGATCCGATCC
ATCTATCAGTTCATAGTAATAACCAAAGCTTAATGTCTATGGATATGAAT
TATCAAACATATCTCAATCCGTGGTCCATTCAAGTGCAATTTATTGACAA
TCTCCATACCCTCATTTCCCCAGTAAGGGTCTGGCGGCCCGAATCTTGGA
AGCTAATGTTATCCGATGAAATTCTGAGACCAATTAAAAACTGGGCTGTT
TACCAACCGGACAGTGAGGAGTGCGTTCCTCGATACAAAGTTGCTCGGTT
GAAAATTATGTGCAGACTCTTTCTGATTGAAGAAACGGGAGAAAAGCGCT
ACATAGGCACTGAAGACGATGATACAAATCAAATCCCATACATTGATGTT
ACTGCAAATACAAAGTTAAATCAATTAAAGTTTTATCCTGAAGATATTTT
CTCAATAGAAAAATCTTTGAACCAGGAATATTTTCTCATAACAGCCAAGC
AAGCTGACAAATCTTCAAGCATTAAATTATTTTCGGAATATTCTCAAACG
CCTCTGACGTCTAAACAAATATCAGCTGATCAGCAATATATATCAGTTGT
TGATCTGATTGTCAACTGCATCGACGCATTGTCCCTAGTTCAACAAAAGC
GATCAAAGAAGTCACTCAACAGTCCATCCTATTACAAGTCAATCACTGTG
TCATTGACCTCAAAGTCACCTAGTAAACCTTGTTATCTCATTGTGTTTGT
AAAGTTCAGCGACGGCTCGATTGAACGGCTATCATTGATCAATATTACGC
AATATCAATTATCTCTAACAAATCCTGATTCTGATATGTTTATTTACAAA
ATAACAGACAATCAATTAGTAACCGTCAGGCATATGCCTCGTGTCAATAT
GTCTCTGTACGAAGACAACTCTTGTAGTCCACCTCGTTTGATTTTCAGCA
AGAAAATTGAATGGGTTACAGTTGAACCGTCGAATAACATCGTTCCAAAC
ACTCATCCATTGTCGTCGACACTATATACCACAATTTCAAAACATAAAAC
CAAATCTTCATTAATTAATGCAGTAGCAATATCTTCTATTAACCTTGTCC
ACATATCCATTTATATTTGTACTGGTTTTGTCGCGTGCATTTTCATTGGC
TGCGTGCTTTTCAAATTGATTTGTTATCGAAATGGCCACGAATCAACTGT
TGAAGATGGAGATGGATCAACAAACTATCAACGATCTGCGTCTGGATTCT
TGGAAGATCCAAATCTCAAAGTTCAAGTGAGATCATCAAACAATAAAAAC
TTCTCGGCATTAAGTAAATGCTCGACCAATTGCTTGAGACAAAATACCAA
CAAAACTCACAACAATGAACCATATCCTCCGTTATTACCACCTCCTGGTC
CGATTGCTACGAACTATGATTTGACTTTATCGGAACCGATATGCAATGAG
TCGGCAGTGGATTCTGGAATTGAGCTTGGAAAACTGTCAAGTTATTCGTC
AAATACTTGTCCGTCAAATGATTTGTGTTGTACGATTGATCTTTATCCTT
CGTGTTCAGGAACTGATGATTCACTGATGCATCAGCCATACCAAAGTGGG
CATTTTGTAAATCCCATGATTGGATTTTATCCACAGAAAGGACTTCATGA
ATCTGAAATTCAATATCAAGAATCAATGATGTTGTCGTCAATGAATTCTA
CGAAAAATATATCAAATCTCCGACATCCGTATTGGTAAAACCTCATTCCA
TAGCCAATCGTGTGCATTTATTTCCTTCTTCTTAAGAGTCTTTCAAGGTT
TTGAAAACTTGAAAATCAAATCATTTCACAACAAAAAATGGATTTATTTT
TTTATTTTGTGTACAAATTCTTCATTTTTAGAAAGTCCGATATTGATTTG
TATAGATTGTGTGAGAAATTGTTTTGTACAAATAAATAATTAATATTGTT
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protein sequence of SMED30008321-orf-1

>SMED30008321-orf-1 ID=SMED30008321-orf-1|Name=SMED30008321-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=987bp
MRSIFNLIISLTILVIIIKFSKTTLSLDIKPTKNVLKLCDHGNSSCVNEL
TQRFVIFNSEINQNITVKLGDLQSALPLNEKSESYNVKIFAERRGYLPPS
NSNEIVINTICQFTYSLAIMSRLELDFCCRVELLIYSKSKSILNPFGVCD
LSLRRDEVHCFIPIKFVYSQYLNEKILPLIGIVHKKSSGSCHFQPEPKDI
YKVSKGLGISFSSRLPVIWQPTHSQKSTILIGIPLYSVKDYENLLIPVRL
VSDLVDLTNQIIRINCQLDPNFVFHSVIVNSENVSIIEDPEIEDENWTIV
DVKLKITPSLDQQLMFLNVTVKLDSMTKNSLKFIKVKCKIIPRNNKHKAR
LYDDATEVKIPLRTSNDLIILPVVKESRDIFSIYSKPNIDFQNDPLQLYI
ITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNPWS
IQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECV
PRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQL
KFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISA
DQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSK
PCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTV
RHMPRVNMSLYEDNSCSPPRLIFSKKIEWVTVEPSNNIVPNTHPLSSTLY
TTISKHKTKSSLINAVAISSINLVHISIYICTGFVACIFIGCVLFKLICY
RNGHESTVEDGDGSTNYQRSASGFLEDPNLKVQVRSSNNKNFSALSKCST
NCLRQNTNKTHNNEPYPPLLPPPGPIATNYDLTLSEPICNESAVDSGIEL
GKLSSYSSNTCPSNDLCCTIDLYPSCSGTDDSLMHQPYQSGHFVNPMIGF
YPQKGLHESEIQYQESMMLSSMNSTKNISNLRHPYW*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR031437TMEM132_M
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031437Transmembrane protein family 132, middle domainPFAMPF16070TMEM132coord: 466..700
e-value: 1.3E-14
score: 54.3
NoneNo IPR availableTMHMMTMhelixcoord: 773..795