GCR160

Overview
NameGCR160
Smed IDSMED30008281
Length (bp)989
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR160 (SMED30008281) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR160 (SMED30008281) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30008281

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30008281 dd_Smed_v4_13742_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008281 dd_Smed_v4_13742_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008281 dd_Smed_v4_13742_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008281 dd_Smed_v6_13742_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR160 vs. TrEMBL
Match: A0A193KUV5 (GCR160 OS=Schmidtea mediterranea OX=79327 GN=gcr160 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.224e-35
Identity = 113/266 (42.48%), Postives = 163/266 (61.28%), Query Frame = 1 
Query:  151 KSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQL-EIFIYVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCS-ESNLKIIFD---YFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIVXXXXXXXXXXXXATFRN 933
            KSLPS  TT +L NQA ID  +C L  ++V +PEQ+    ++ +   C   N   I+W ++ AG+ NL+FTA DRYWA+I PL+Y  ++K+     ++I Y  S  I IP  F+ S  N + ES      D    F+K  SI+W LSIY IP+ +MIY+Y  I+F  ++ +  K+  + KT+TKA ++ITI+FL TI ++ +  +L  +KV+ Y F   G  ++   L G+FL SFNSC+NPVVLF+IV+  R R  KLL   FRN
Sbjct:   33 KSLPSQYTTVVLRNQAFIDCFVCFLTFLFVVLPEQIITKNLFFDLFFCHIWNGQAIYWWMVLAGVFNLIFTAFDRYWAIITPLSYGNNLKKKVKLSYLIIYLVSGIITIPAMFQTSLKNHTCESTYAFEGDAVTAFYKGFSIVWFLSIYGIPATIMIYLYSRIVFNIRRNVNAKRKFTFKTITKAAIIITIVFLVTIAYDAIYYMLGYFKVISYEF---GTPIQ---LLGVFLTSFNSCINPVVLFIIVRNIRIRCYKLLGIIFRN 292
[Close]
BLAST of GCR160 vs. TrEMBL
Match: A0A193KUF5 (GCR145 OS=Schmidtea mediterranea OX=79327 GN=gcr145 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.725e-16
Identity = 80/249 (32.13%), Postives = 129/249 (51.81%), Query Frame = 1 
Query:  151 KSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQLEIFI-YVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVN--CSE--SNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKTL--TKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFL 876
            +SL S  T  +L NQ++IDS +CL+ L+ + +P+     I +++   C       + +  I AG  NLVFTAVDR+WAV+ P  Y ++I R  I  FV  Y +      P++ E   +N  C+   S+L      FFK +S +W+L  Y IP  ++I++Y  +     K     + +   T+  TKA +++ IIF+ T  F+    +L     ++Y F+   +      L G+ L + NS VNPVV F+
Sbjct:   39 RSLKSKYTNCILRNQSIIDSSLCLITLLALNLPKTSSSGILFIDQISCSIWTGQYLEYCFILAGEYNLVFTAVDRFWAVVTPSFYQKNINRKIILSFVFIYTFPVIFNWPQFIEFRLINGTCNSDYSDLGPNIVMFFKVNSTMWVLFQYIIPVAIIIFLYWKVYKAMNKLKSNNEETKKATMKFTKATMIVVIIFMLTCFFDPFYYMLGYLGAVEYVFNSPLQ------LTGISLITINSAVNPVVYFI 281
[Close]
BLAST of GCR160 vs. TrEMBL
Match: A0A1I8HGG3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.885e-13
Identity = 54/207 (26.09%), Postives = 98/207 (47.34%), Query Frame = 1 
Query:  289 CISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNY----FRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKT-----LTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIV 882
            C  IN   ++W  + + + NLV  AVDRY+AV  P+ Y    FR    + + L   ++A +   +    F++   +CS      +F   FK +  +W   +Y +P   ++ +Y      ++   Q   NS  +      LTK+ V+IT+IF+    ++ +  +L    +++Y +    +      L G+ L + NSC NPVV  +I+
Sbjct:  345 CHVINSQLLYWYFVFSSVQNLVLIAVDRYFAVFRPVRYKTLSFRSAALHLVGLHCYSFAVNIIDIFSVEFDRDSNHCSSEITHQVFKKVFKVYGFLWFFLVYLLPVAAVVCLY--SAIVYRLSNQGLHNSRFRQRAATELTKSAVIITVIFIVCFSYDCIYYLLGHLDLVEYIYSSPTQ------LIGVALVTINSCANPVVYCIIM 543
[Close]
BLAST of GCR160 vs. TrEMBL
Match: A0A3S5A5E5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS938 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.361e-7
Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 1 
Query:  313 IFWMVIQAGILNLVFTAVDRYWAVILPLNY--FRDIKRN-TIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDY--FFKTHSIIWLLSIYAIPSIVMIYIY------LTXXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLW-GMFLHSFNSCVNPVVLFLIV 882
            ++W  +   + NL+  AVDRY+AV+ P+ Y   +  + N  IFL  +T A   ++ I   F+ ++ N  E   +   DY  FF  + I W   +Y IP + +  +Y      L+   +        +  +   LTK+ VVIT+IF+ T  ++ +  +L    V+ Y F+       TF    G+FL   NSC NP+V  LI+
Sbjct:    2 VYWFFVFLSVYNLMLIAVDRYFAVLQPVRYKSLKMCQANLCIFLLHVTSAVVNSVDI---FQMNY-NPVEKACESQVDYIAFFNVYGIAWFFIVYLIPVLTVFCLYGRVICHLSRGSEAAASTAKCQQRAATELTKSAVVITVIFVITFSYDSIYYLLGSLDVISYSFN-------TFEQTIGVFLVGLNSCANPLVYCLIM 192
[Close]
BLAST of GCR160 vs. TrEMBL
Match: A0A1I8FSX6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 6.022e-7
Identity = 50/201 (24.88%), Postives = 90/201 (44.78%), Query Frame = 1 
Query:  289 CISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLT---XXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIV 882
            C  IN   ++W  + + + NLV  AVDRY+AV  PL   R        L   ++A +   +    F++   +CS      +F   FK +  +W   +Y +P   ++ +Y      +          +  +   LTK+ V+IT+IF+    ++ +  +L    +++Y +           L G+ L + NSC NPVV  +I+
Sbjct:  331 CHVINSQLLYWYFVFSSVQNLVLIAVDRYFAVFRPLPERRSASGRPA-LHCYSFAVNIIDIFSVEFDRDSNHCSSEITHQVFKKVFKVYGFLWFFLVYLLPVAAVVCLYSAIVYRLSNQGLHNSRFRQRAATELTKSAVIITVIFIVCFSYDCIYYLLGHLDLVEYIYSS------PTQLIGVALVTINSCANPVVYCIIM 524
[Close]
BLAST of GCR160 vs. Planmine SMEST
Match: SMESG000008854.1 (SMESG000008854.1)

HSP 1 Score: 479.945 bits (1234), Expect = 3.693e-172
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 1 
Query:   58 MFSHKLCFSFIGGCQXXXXXXXXXXXXXXTTKSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQLEIFIYVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIVXXXXXXXXXXXXATFRNF 936
            MFSHKLCFSFIGGCQIFGLLMNILLLIVLTTKSLPSLRTTALLFNQALIDSCICLLLLIYVTIPEQLEIFIYVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTIIFKFQKKIQLKKNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIVKKFRSRFRKLLKATFRNF
Sbjct:    1 MFSHKLCFSFIGGCQIFGLLMNILLLIVLTTKSLPSLRTTALLFNQALIDSCICLLLLIYVTIPEQLEIFIYVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTIIFKFQKKIQLKKNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIVKKFRSRFRKLLKATFRNF 293
[Close]
BLAST of GCR160 vs. Planmine SMEST
Match: SMESG000005776.1 (SMESG000005776.1)

HSP 1 Score: 114.775 bits (286), Expect = 2.586e-30
Identity = 90/207 (43.48%), Postives = 130/207 (62.80%), Query Frame = 1 
Query:  325 VIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCS-ESNLKIIFD---YFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIVXXXXXXXXXXXXATFRN 933
            ++ AG+ NL+FTA DRYWA+I PL+Y  ++K+     ++I Y  S  I IP  F+ S  N + ES      D    F+K  SI+W LSIY IP+ +MIY+Y  I+F  ++ +  K+  + KT+TKA ++ITI+FL TI ++ +  +L  +KV+ Y F   G  ++   L G+FL SFNSC+NPVVLF+IV+  R R  KLL   FRN
Sbjct:    1 MVLAGVFNLIFTAFDRYWAIITPLSYGNNLKKKVKLSYLIIYLVSGIITIPAMFQTSLKNHTCESTYAFEGDAVTAFYKGFSIVWFLSIYGIPATIMIYLYSRIVFNIRRNVNAKRKFTFKTITKAAIIITIVFLVTIAYDAIYYMLGYFKVISYEF---GTPIQ---LLGVFLTSFNSCINPVVLFIIVRNIRIRCYKLLGIIFRN 201
[Close]
BLAST of GCR160 vs. Planmine SMEST
Match: SMESG000037926.1 (SMESG000037926.1)

HSP 1 Score: 107.842 bits (268), Expect = 8.401e-27
Identity = 82/258 (31.78%), Postives = 134/258 (51.94%), Query Frame = 1 
Query:  151 KSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQLEIFIYVNAEKCISINVLKI------FWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCS-ESNLK---IIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKT----LTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIV 882
            + L S  TT ++ +QA+ID  IC + ++ V  P  L      +    + I + +I      FW ++ + + NL+FTA+DRYWAV+ P  Y   + R  +  + I       I +P Y +  + N S  S L+   II    FK +S+IW LSIY IP+ ++IYIY  +I +    +Q    S++KT     TK  +V T+IF+ TI ++ +  +L    +  Y F+   + +      G+FL + NS VNP++ F +V
Sbjct:   41 RGLDSKFTTFIVRSQAVIDMMICFMTMMLVIFPNPLN-----SGNLVLDIIICRIWTSQATFWWLVLSSVYNLLFTAIDRYWAVLKPQTYKHHMNRKIVLAYSIIIGMCGFITLPSYLQSKYYNGSCTSELQYSGIIVSNLFKAYSVIWFLSIYGIPTPILIYIYTKVILQLTSSLQGDSGSNLKTAMRSFTKGTMVTTLIFILTISYDAIYYMLGYLGIAVYIFNSPPQVV------GVFLTAVNSSVNPIIFFALV 287
[Close]
BLAST of GCR160 vs. Planmine SMEST
Match: SMESG000037908.1 (SMESG000037908.1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.914e-22
Identity = 80/253 (31.62%), Postives = 143/253 (56.52%), Query Frame = 1 
Query:  151 KSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQLEIF-IYVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNCSE-SNLKIIFD---YFFKTHSIIWLLSIYAIPSIVMIYIYL----TXXXXXXXXXXXXXNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFLIV 882
            ++L S  T  LL +Q+LIDS +C++ +I V  P+ +    + V++  C       IFW  + A I NL+F AVDRY AVI+P +Y   IK+   F + I +  +   VIP YF+ +F N +  + +  + +   + FK+ SI W +++Y  P+ ++I++Y      +   F++  ++   +S+K+ TK+ ++ TIIFL TI ++ +  +L    V++Y  +   +++      G+FL S NS  NP+V F++V
Sbjct:   39 RALESKFTNYLLRSQSLIDSVVCIVTIIIVIFPDPMTSGNVNVDSVFCHIWTSQAIFWWTVLASIYNLIFLAVDRYSAVIIPQSYKNKIKKKIFFAYSIIFGCTGLSVIPAYFQYTFQNGTCLTQMDFVGESAKFAFKSFSIYWFIAVYGFPAPLLIFLYSKIIWKLYESFKRDKRISMQASMKSFTKSTLINTIIFLITIAYDAMYYMLGYLGVVEYVLYSPTQRV------GVFLTSVNSSTNPLVFFILV 285
[Close]
BLAST of GCR160 vs. Planmine SMEST
Match: SMESG000003474.1 (SMESG000003474.1)

HSP 1 Score: 88.9669 bits (219), Expect = 4.796e-20
Identity = 80/249 (32.13%), Postives = 129/249 (51.81%), Query Frame = 1 
Query:  151 KSLPSLRTTALLFNQALIDSXXXXXXXXYVTIPEQLEIFI-YVNAEKCISINVLKIFWMVIQAGILNLVFTAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVN--CSE--SNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTXXXXXXXXXXXXXNSSIKTL--TKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLETFYLWGMFLHSFNSCVNPVVLFL 876
            +SL S  T  +L NQ++IDS +CL+ L+ + +P+     I +++   C       + +  I AG  NLVFTAVDR+WAV+ P  Y ++I R  I  FV  Y +      P++ E   +N  C+   S+L      FFK +S +W+L  Y IP  ++I++Y  +     K     + +   T+  TKA +++ IIF+ T  F+    +L     ++Y F+   +      L G+ L + NS VNPVV F+
Sbjct:   39 RSLKSKYTNCILRNQSIIDSSLCLITLLALNLPKTSSSGILFIDQISCSIWTGQYLEYCFILAGEYNLVFTAVDRFWAVVTPSFYQKNINRKIILSFVFIYTFPVIFNWPQFIEFRLINGTCNSDYSDLGPNIVMFFKVNSTMWVLFQYIIPVAIIIFLYWKVYKAMNKLKSNNEETKKATMKFTKATMIVVIIFMLTCFFDPFYYMLGYLGAVEYVFNSPLQ------LTGISLITINSAVNPVVYFI 281
The following BLAST results are available for this feature:
BLAST of GCR160 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUV51.224e-3542.48GCR160 OS=Schmidtea mediterranea OX=79327 GN=gcr16... [more]
A0A193KUF51.725e-1632.13GCR145 OS=Schmidtea mediterranea OX=79327 GN=gcr14... [more]
A0A1I8HGG34.885e-1326.09Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A3S5A5E51.361e-729.70G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A1I8FSX66.022e-724.88Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of GCR160 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GCR160 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000008854.13.693e-172100.00SMESG000008854.1[more]
SMESG000005776.12.586e-3043.48SMESG000005776.1[more]
SMESG000037926.18.401e-2731.78SMESG000037926.1[more]
SMESG000037908.11.914e-2231.62SMESG000037908.1[more]
SMESG000003474.14.796e-2032.13SMESG000003474.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008281 ID=SMED30008281|Name=GCR160|organism=Schmidtea mediterranea sexual|type=transcript|length=989bp
CCCTGTACAAGATTCTGTCGTTCGACAAACATACAATAATCAAATGCAAA
AATACAAATGTTTAGCCATAAACTTTGTTTTTCTTTTATTGGAGGATGTC
AAATATTTGGGTTGTTAATGAATATTCTTTTACTGATTGTTTTAACCACA
AAAAGTTTGCCATCCTTGAGGACAACCGCTTTATTGTTCAACCAAGCCTT
GATAGATTCCTGTATTTGTTTATTATTATTGATTTACGTCACCATTCCAG
AGCAATTGGAAATATTTATATATGTCAATGCAGAAAAATGTATTTCAATA
AACGTTTTAAAAATATTTTGGATGGTCATCCAAGCAGGAATACTCAATCT
GGTGTTTACGGCAGTCGACCGATATTGGGCTGTAATATTGCCTTTGAATT
ATTTCAGAGATATAAAAAGAAACACTATATTCCTATTTGTCATAACGTAC
GCATATAGCGCAACTATTGTCATTCCGAAATATTTTGAAAAGTCATTTGT
CAACTGCAGTGAAAGTAATTTAAAAATTATTTTTGATTATTTTTTTAAAA
CTCATTCGATTATTTGGCTCCTATCAATTTATGCCATCCCGTCTATAGTT
ATGATCTATATTTATTTGACAATTATTTTCAAATTTCAAAAGAAAATCCA
ATTGAAAAAAAATTCATCAATAAAAACTTTAACAAAAGCTTTGGTAGTTA
TAACCATAATATTTCTTTCAACCATTCCTTTTAATGTTGTCTGTGAAATT
TTAGACAAATGGAAAGTCATGGATTATCGATTTCATAAAGATGGCAGAAA
ATTAGAAACGTTTTATCTCTGGGGAATGTTTCTTCACTCCTTCAATAGCT
GTGTCAATCCAGTCGTTTTATTCCTCATTGTCAAGAAATTCCGCTCTAGA
TTTCGTAAACTTCTTAAAGCTACTTTTCGAAATTTTTAATTCAATTATTT
TGTGAACACTATTTTTTAAATACTGATTAAATTTAACGG
back to top

protein sequence of SMED30008281-orf-1

>SMED30008281-orf-1 ID=SMED30008281-orf-1|Name=SMED30008281-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=294bp
MFSHKLCFSFIGGCQIFGLLMNILLLIVLTTKSLPSLRTTALLFNQALID
SCICLLLLIYVTIPEQLEIFIYVNAEKCISINVLKIFWMVIQAGILNLVF
TAVDRYWAVILPLNYFRDIKRNTIFLFVITYAYSATIVIPKYFEKSFVNC
SESNLKIIFDYFFKTHSIIWLLSIYAIPSIVMIYIYLTIIFKFQKKIQLK
KNSSIKTLTKALVVITIIFLSTIPFNVVCEILDKWKVMDYRFHKDGRKLE
TFYLWGMFLHSFNSCVNPVVLFLIVKKFRSRFRKLLKATFRNF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0004871signal transducer activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 167..190
score: 20.76
coord: 207..231
score: 16.33
coord: 253..279
score: 26.31
coord: 39..60
score: 21.66
coord: 87..109
score: 29.49
coord: 6..30
score: 18.52
IPR000276G protein-coupled receptor, rhodopsin-likeSMARTSM013817TM_GPCR_Srsx_2coord: 15..286
e-value: 9.4E-4
score: -54.2
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 22..270
e-value: 1.6E-13
score: 50.6
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 93..109
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 1..292
e-value: 1.2E-29
score: 105.3
NoneNo IPR availablePANTHERPTHR45698FAMILY NOT NAMEDcoord: 14..287
NoneNo IPR availableCDDcd006377tm_classA_rhodopsin-likecoord: 16..270
e-value: 1.92714E-15
score: 72.6954
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 3..289
NoneNo IPR availableTMHMMTMhelixcoord: 122..139
NoneNo IPR availableTMHMMTMhelixcoord: 211..233
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 10..29
NoneNo IPR availableTMHMMTMhelixcoord: 168..190
NoneNo IPR availableTMHMMTMhelixcoord: 253..275
NoneNo IPR availableTMHMMTMhelixcoord: 42..64
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 21..271
score: 19.589