SMED30008271

Overview
NameSMED30008271
Smed IDSMED30008271
Length (bp)540
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008271 (SMED30008271) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008271 (SMED30008271) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008271 (SMED30008271) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008271 (SMED30008271) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30008271

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008271SMESG000072142.1 SmedASXL_061545SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30008271SMESG000072142.1 dd_Smed_v4_1267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30008271SMESG000072142.1 dd_Smed_v6_1267_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30008271SMESG000072142.1 OX_Smed_1.0.02114ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008271 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006346:18002..18639 -2667
Homology
[Close]
BLAST of SMED30008271 vs. TrEMBL
Match: I1ZI82 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.294e-18
Identity = 46/117 (39.32%), Postives = 63/117 (53.85%), Query Frame = 2 
Query:  101 IQIGFFMTLALLNVECVS--KSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELY 445
            I     +  ++L V C S    +    C  +L +ID ECD KY  LD  CD  Y  +H+ CDI Y  LSGRC+  ++  CI +YG LD  CL +   +   C ++YN +D HCD LY
Sbjct:    1 ILFAAVLIQSMLVVHCASVKAVEKKGFCSGILHRIDTECDDKYRRLDRECDHSYESIHEVCDIKYTMLSGRCRVKLTEDCIKRYGNLDHSCLESLRNIQEKCQEKYNRVDDHCDALY 117
[Close]
BLAST of SMED30008271 vs. TrEMBL
Match: J7GX15 (PROG-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.357e-6
Identity = 32/99 (32.32%), Postives = 48/99 (48.48%), Query Frame = 2 
Query:  170 ADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCE 466
            ADC+ + ++ D+ CD  YL LD  CD RY          +D    +C +    +C  +   L+    G YD +D  CD  Y+ LD +CD  Y   ++CE
Sbjct:  115 ADCMKLYEETDKYCDPLYLKLDKDCDCRYVKALNDYTKRWDSYHSKCGSKYDKKCRTELRALENWFNGLYDSIDGHCDAEYDALDKYCDVEYNKVDQCE 213
[Close]
BLAST of SMED30008271 vs. Planmine SMEST
Match: SMESG000072142.1 (SMESG000072142.1)

HSP 1 Score: 260.766 bits (665), Expect = 1.191e-90
Identity = 129/130 (99.23%), Postives = 130/130 (100.00%), Query Frame = 2 
Query:   89 MKLMIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE 478
            MKLMIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYG+LDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE
Sbjct:    1 MKLMIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGRLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE 130
[Close]
BLAST of SMED30008271 vs. Planmine SMEST
Match: SMESG000072142.1 (SMESG000072142.1)

HSP 1 Score: 255.373 bits (651), Expect = 1.583e-88
Identity = 126/127 (99.21%), Postives = 127/127 (100.00%), Query Frame = 2 
Query:   98 MIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE 478
            MIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYG+LDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE
Sbjct:    1 MIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGRLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNSE 127
[Close]
BLAST of SMED30008271 vs. Planmine SMEST
Match: SMESG000024754.1 (SMESG000024754.1)

HSP 1 Score: 201.06 bits (510), Expect = 4.931e-67
Identity = 99/129 (76.74%), Postives = 110/129 (85.27%), Query Frame = 2 
Query:   89 MKLMIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNS 475
            MKLMIQIG  + LAL+NVECVSKSK + DCLTMLDKID ECD++YLVLD HCD  YA LHK CDI YD LSGRCK  +SMRCI KYGKLD KCL AYD+VD++CDKRYNVLDTHCD LYK+QEKCE+NS
Sbjct:    1 MKLMIQIGLLVVLALINVECVSKSKKAFDCLTMLDKIDNECDKRYLVLDGHCDRSYADLHKNCDIKYDLLSGRCKGKLSMRCIRKYGKLDGKCLRAYDKVDSYCDKRYNVLDTHCDGLYKLQEKCENNS 129
[Close]
BLAST of SMED30008271 vs. Planmine SMEST
Match: SMESG000024821.1 (SMESG000024821.1)

HSP 1 Score: 163.696 bits (413), Expect = 1.697e-52
Identity = 80/104 (76.92%), Postives = 90/104 (86.54%), Query Frame = 2 
Query:  164 NSADCLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCEDNS 475
            ++ DCLTMLDKID ECD++YLVLD HCD  YA LHK CDI YD LSGRCK  +SMRCI KYGKLD KCLGAYD+VD++CDKRYNVLDTHCD LYK+QEKCE+NS
Sbjct:    5 SAFDCLTMLDKIDNECDKRYLVLDGHCDRSYADLHKNCDIKYDLLSGRCKGKLSMRCIRKYGKLDGKCLGAYDKVDSYCDKRYNVLDTHCDGLYKLQEKCENNS 108
[Close]
BLAST of SMED30008271 vs. Planmine SMEST
Match: SMESG000021388.1 (SMESG000021388.1)

HSP 1 Score: 124.405 bits (311), Expect = 1.258e-36
Identity = 67/136 (49.26%), Postives = 82/136 (60.29%), Query Frame = 2 
Query:   95 LMIQIGFFMTLALLNVECVSKSKNSAD------------CLTMLDKIDRECDRKYLVLDNHCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRVDTFCDKRYNVLDTHCDELYKIQEKCE 466
            L I   F +TLA+L V C+   K   D            C   LDK+D ECD+KY +LDN C+  Y  L+KTCD+NY KLSGRC+  +S  CI KYGKLDR C+ AY  +D  CDKRY  LD +CD LY  +E CE
Sbjct:    2 LRIIYAFSVTLAVLRVNCLESPKVFKDEIVNNYEKKWMKCTKFLDKVDAECDKKYDLLDNDCNGSYKQLYKTCDVNYLKLSGRCQMRLSKNCIKKYGKLDRHCIKAYGEIDESCDKRYAKLDPYCDALYTKEENCE 137
The following BLAST results are available for this feature:
BLAST of SMED30008271 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
I1ZI822.294e-1839.32Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
J7GX152.357e-632.32PROG-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=... [more]
back to top
BLAST of SMED30008271 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30008271 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072142.11.191e-9099.23SMESG000072142.1[more]
SMESG000072142.11.583e-8899.21SMESG000072142.1[more]
SMESG000024754.14.931e-6776.74SMESG000024754.1[more]
SMESG000024821.11.697e-5276.92SMESG000024821.1[more]
SMESG000021388.11.258e-3649.26SMESG000021388.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008271 ID=SMED30008271|Name=SMED30008271|organism=Schmidtea mediterranea sexual|type=transcript|length=540bp
CGAAATTGATGAAAACTTGATTCCAGCCTACCCGATTTATGGGAGGTATA
AATTGCTCACAACACTCATTCAATAACAAATTTCAACCATGAAATTGATG
ATCCAAATCGGTTTCTTCATGACATTGGCATTGCTCAATGTCGAATGCGT
GAGCAAGTCAAAAAACAGTGCTGATTGCTTGACGATGCTGGATAAGATTG
ACAGAGAATGCGACAGGAAATATTTGGTACTCGACAACCATTGTGATAGC
CGTTACGCCCACTTGCACAAGACATGCGACATTAATTATGATAAACTAAG
TGGTCGGTGCAAAAATAATATGTCAATGCGTTGCATTCTTAAGTATGGGA
AGCTGGATAGGAAATGTTTGGGAGCATATGATAGGGTCGATACCTTTTGT
GACAAAAGGTACAATGTCTTGGACACTCATTGCGATGAATTGTACAAGAT
ACAAGAGAAATGTGAGGATAACAGTGAGTGAAAAGTGGTTAAATGGTTTT
GATCCGTAATAGTTTGCTTTTTCTCTAGAGTCTAGTTGAA
back to top

protein sequence of SMED30008271-orf-1

>SMED30008271-orf-1 ID=SMED30008271-orf-1|Name=SMED30008271-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=131bp
MKLMIQIGFFMTLALLNVECVSKSKNSADCLTMLDKIDRECDRKYLVLDN
HCDSRYAHLHKTCDINYDKLSGRCKNNMSMRCILKYGKLDRKCLGAYDRV
DTFCDKRYNVLDTHCDELYKIQEKCEDNSE*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.62