28S ribosomal protein S18c, mitochondrial

Overview
Name28S ribosomal protein S18c, mitochondrial
Smed IDSMED30008248
Length (bp)416
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 28S ribosomal protein S18c, mitochondrial (SMED30008248) t-SNE clustered cells

Violin plots show distribution of expression levels for 28S ribosomal protein S18c, mitochondrial (SMED30008248) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 28S ribosomal protein S18c, mitochondrial (SMED30008248) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 28S ribosomal protein S18c, mitochondrial (SMED30008248) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008248

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008248SMESG000018393.1 SmedASXL_019344SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Match: MRPS18C (mitochondrial ribosomal protein S18C [Source:HGNC Symbol;Acc:HGNC:16633])

HSP 1 Score: 79.337 bits (194), Expect = 3.318e-19
Identity = 39/95 (41.05%), Postives = 59/95 (62.11%), Query Frame = 1 
Query:   73 RNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            R  + + +   DLP+ +M++P+K+P+ +CI+C   +  DYKNV+LLSQF+SP +G  Y  HITGLC    ++I   I RA+  G M    K+  Y
Sbjct:   38 RGCSQQVSSNEDLPI-SMENPYKEPLKKCILCGKHV--DYKNVQLLSQFVSPFTGCIYGRHITGLCGKKQKEITKAIKRAQIMGFMPVTYKDPAY 129
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Match: MRPS18C (mitochondrial ribosomal protein S18C [Source:HGNC Symbol;Acc:HGNC:16633])

HSP 1 Score: 45.0542 bits (105), Expect = 2.236e-6
Identity = 23/51 (45.10%), Postives = 29/51 (56.86%), Query Frame = 1 
Query:  205 LLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            LLSQF+SP +G  Y  HITGLC    ++I   I RA+  G M    K+  Y
Sbjct:   51 LLSQFVSPFTGCIYGRHITGLCGKKQKEITKAIKRAQIMGFMPVTYKDPAY 101
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Match: MRPS18C (mitochondrial ribosomal protein S18C [Source:HGNC Symbol;Acc:HGNC:16633])

HSP 1 Score: 45.0542 bits (105), Expect = 2.290e-6
Identity = 23/51 (45.10%), Postives = 29/51 (56.86%), Query Frame = 1 
Query:  205 LLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            LLSQF+SP +G  Y  HITGLC    ++I   I RA+  G M    K+  Y
Sbjct:   50 LLSQFVSPFTGCIYGRHITGLCGKKQKEITKAIKRAQIMGFMPVTYKDPAY 100
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Match: MRPS18B (mitochondrial ribosomal protein S18B [Source:HGNC Symbol;Acc:HGNC:14516])

HSP 1 Score: 46.2098 bits (108), Expect = 3.784e-6
Identity = 23/67 (34.33%), Postives = 41/67 (61.19%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            N C +C +  + +D++NV+LL QF+   +G  + A  TG+C   H+++   I +ARD GL+ + I +
Sbjct:  103 NPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFYAPYTGVCVKQHKRLTQAIQKARDHGLLIYHIPQ 169
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Match: MRPS18B (mitochondrial ribosomal protein S18B [Source:HGNC Symbol;Acc:HGNC:14516])

HSP 1 Score: 46.2098 bits (108), Expect = 3.784e-6
Identity = 23/67 (34.33%), Postives = 41/67 (61.19%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            N C +C +  + +D++NV+LL QF+   +G  + A  TG+C   H+++   I +ARD GL+ + I +
Sbjct:  103 NPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFYAPYTGVCVKQHKRLTQAIQKARDHGLLIYHIPQ 169
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Celegans
Match: mrps-18.C (Mitochondrial Ribosomal Protein, Small [Source:UniProtKB/TrEMBL;Acc:Q22483])

HSP 1 Score: 77.411 bits (189), Expect = 5.370e-19
Identity = 36/85 (42.35%), Postives = 52/85 (61.18%), Query Frame = 1 
Query:  103 NDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            +D PV   ++P+ K   +C++C   + LDYKN RLL QF+S  SGR YD HITGLC+   +K+   I ++R  G M   +K+  Y
Sbjct:   36 DDEPVILENNPYTKEPRKCLLCSTGVELDYKNSRLLQQFVSTFSGRVYDRHITGLCDENKKKLIEAIAKSRRAGFMPIFVKDPKY 120
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Fly
Match: mRpS18C (gene:FBgn0039765 transcript:FBtr0085625)

HSP 1 Score: 73.559 bits (179), Expect = 2.756e-17
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1 
Query:  106 DLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLM 330
            DLP+ ++ +P++K   +CI+C + I   YKNV+LLSQF SP +GR Y  HITGLC    E+++  I RA+ C LM
Sbjct:   40 DLPI-DIKNPYEKDPQQCILCKHSIEPHYKNVKLLSQFQSPYTGRIYGRHITGLCKRRQEQVEQAILRAQQCLLM 113
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Fly
Match: mRpS18B (gene:FBgn0032849 transcript:FBtr0081338)

HSP 1 Score: 54.6842 bits (130), Expect = 1.128e-9
Identity = 25/65 (38.46%), Postives = 39/65 (60.00%), Query Frame = 1 
Query:  151 NRCIVCVND-IPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C +C ++ + LDY+N  LL QFISP SG       TGLC   H ++ + + +ARD G + +++
Sbjct:  121 NPCPICRDEYLVLDYRNTELLEQFISPHSGDVLSYSKTGLCQKSHLRLMVAVQQARDSGYLTYDV 185
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Fly
Match: mRpS18B (gene:FBgn0032849 transcript:FBtr0081339)

HSP 1 Score: 54.6842 bits (130), Expect = 1.128e-9
Identity = 25/65 (38.46%), Postives = 39/65 (60.00%), Query Frame = 1 
Query:  151 NRCIVCVND-IPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C +C ++ + LDY+N  LL QFISP SG       TGLC   H ++ + + +ARD G + +++
Sbjct:  121 NPCPICRDEYLVLDYRNTELLEQFISPHSGDVLSYSKTGLCQKSHLRLMVAVQQARDSGYLTYDV 185
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Zebrafish
Match: mrps18c (mitochondrial ribosomal protein S18C [Source:ZFIN;Acc:ZDB-GENE-060503-609])

HSP 1 Score: 82.8037 bits (203), Expect = 7.551e-21
Identity = 42/113 (37.17%), Postives = 69/113 (61.06%), Query Frame = 1 
Query:   28 LFRSKFIFNVIKKNVRNFTSETN---IENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            L +   I     + VR+ T+       ++D+P+  MD+P+K+P   CI+C  ++ +D+KNV+LLSQFISP +GR Y  HITGLC    +++   I ++R  G M+  +K+ L+
Sbjct:   14 LPKQPVIPRATAQCVRSLTTSQQDPASKDDMPI-KMDNPYKQPAKTCILC--NVTVDFKNVQLLSQFISPHTGRIYGRHITGLCGRKQKEVTKAIKKSRSMGFMSVTLKDPLF 123
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Zebrafish
Match: mrps18c (mitochondrial ribosomal protein S18C [Source:ZFIN;Acc:ZDB-GENE-060503-609])

HSP 1 Score: 82.8037 bits (203), Expect = 7.551e-21
Identity = 42/113 (37.17%), Postives = 69/113 (61.06%), Query Frame = 1 
Query:   28 LFRSKFIFNVIKKNVRNFTSETN---IENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            L +   I     + VR+ T+       ++D+P+  MD+P+K+P   CI+C  ++ +D+KNV+LLSQFISP +GR Y  HITGLC    +++   I ++R  G M+  +K+ L+
Sbjct:   14 LPKQPVIPRATAQCVRSLTTSQQDPASKDDMPI-KMDNPYKQPAKTCILC--NVTVDFKNVQLLSQFISPHTGRIYGRHITGLCGRKQKEVTKAIKKSRSMGFMSVTLKDPLF 123
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Zebrafish
Match: mrps18b (mitochondrial ribosomal protein S18B [Source:ZFIN;Acc:ZDB-GENE-050417-278])

HSP 1 Score: 44.669 bits (104), Expect = 6.879e-6
Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = 1 
Query:  139 KKPMNRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLM 330
            KK  N C +C + +I + YKNV LL QF  P +G  +D  +TG+C    + +   I  A+D GL+
Sbjct:  113 KKCANPCPICRDPNIIIHYKNVNLLQQFFCPETGVVHDPTVTGVCMKQQKLLSKAIETAKDFGLL 177
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Xenopus
Match: mrps18c (mitochondrial ribosomal protein S18C [Source:NCBI gene;Acc:549846])

HSP 1 Score: 68.5514 bits (166), Expect = 2.988e-15
Identity = 34/88 (38.64%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:   94 NIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
             +  D+P   M++P+K P  +CI+C   + +DYKN +LLSQFISP +G  +  HITGLC      +   I RA   G M    K+  +
Sbjct:   38 QVHEDMPQ-QMENPYKDPPKKCILC--GVTVDYKNTQLLSQFISPHTGHIFGRHITGLCGRKQRAVSKAIKRAHIMGFMPVTYKDPAF 122
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Xenopus
Match: ENSXETT00000017678.1 (mitochondrial ribosomal protein S18B [Source:NCBI gene;Acc:100489063])

HSP 1 Score: 47.3654 bits (111), Expect = 7.862e-7
Identity = 24/65 (36.92%), Postives = 36/65 (55.38%), Query Frame = 1 
Query:  151 NRCIVCVND-IPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C +C +  + L+Y+NV+LL QFI P +G  YD   TG+C    + +   I  A D GL+   +
Sbjct:   45 NPCPICRDQKLGLNYRNVKLLQQFICPHTGTVYDPTKTGVCMKQQKALVKAITEATDHGLLPAHV 109
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Mouse
Match: Mrps18c (mitochondrial ribosomal protein S18C [Source:MGI Symbol;Acc:MGI:1915985])

HSP 1 Score: 77.7962 bits (190), Expect = 8.863e-19
Identity = 42/108 (38.89%), Postives = 60/108 (55.56%), Query Frame = 1 
Query:   34 RSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            R     +VI +   +   +     DLPV  M++P+K+P+ +C++C   +  DYKNV+LLSQFISP +G  Y  HITGLC     +I   I RA+  G M    K+  Y
Sbjct:   26 RGTQAVSVIWRRCFSQFEQVTSNEDLPV-PMENPYKEPLKKCVLCEKRV--DYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQKMGFMPVTYKDPAY 130
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Mouse
Match: Mrps18c (mitochondrial ribosomal protein S18C [Source:MGI Symbol;Acc:MGI:1915985])

HSP 1 Score: 73.9442 bits (180), Expect = 2.147e-17
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 1 
Query:  103 NDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCG 324
             DLPV  M++P+K+P+ +C++C   +  DYKNV+LLSQFISP +G  Y  HITGLC     +I   I RA+  G
Sbjct:   49 EDLPV-PMENPYKEPLKKCVLCEKRV--DYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQKMG 119
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Mouse
Match: Mrps18b (mitochondrial ribosomal protein S18B [Source:MGI Symbol;Acc:MGI:1914223])

HSP 1 Score: 51.2174 bits (121), Expect = 4.711e-8
Identity = 23/67 (34.33%), Postives = 43/67 (64.18%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            N C +C +  + +D++NV+LL QF+   +G  + A  TG+C   H+K+   I +AR+CGL+++ + +
Sbjct:  101 NPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARECGLLSYYVPQ 167
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Mouse
Match: Mrps18b (mitochondrial ribosomal protein S18B [Source:MGI Symbol;Acc:MGI:1914223])

HSP 1 Score: 45.0542 bits (105), Expect = 3.003e-6
Identity = 19/52 (36.54%), Postives = 34/52 (65.38%), Query Frame = 1 
Query:  193 KNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            +NV+LL QF+   +G  + A  TG+C   H+K+   I +AR+CGL+++ + +
Sbjct:   24 QNVKLLEQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARECGLLSYYVPQ 75
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Match: sp|Q9Y3D5|RT18C_HUMAN (28S ribosomal protein S18c, mitochondrial OS=Homo sapiens OX=9606 GN=MRPS18C PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.593e-18
Identity = 39/95 (41.05%), Postives = 59/95 (62.11%), Query Frame = 1 
Query:   73 RNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            R  + + +   DLP+ +M++P+K+P+ +CI+C   +  DYKNV+LLSQF+SP +G  Y  HITGLC    ++I   I RA+  G M    K+  Y
Sbjct:   38 RGCSQQVSSNEDLPI-SMENPYKEPLKKCILCGKHV--DYKNVQLLSQFVSPFTGCIYGRHITGLCGKKQKEITKAIKRAQIMGFMPVTYKDPAY 129
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Match: sp|Q8R2L5|RT18C_MOUSE (28S ribosomal protein S18c, mitochondrial OS=Mus musculus OX=10090 GN=Mrps18c PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.200e-18
Identity = 42/108 (38.89%), Postives = 60/108 (55.56%), Query Frame = 1 
Query:   34 RSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            R     +VI +   +   +     DLPV  M++P+K+P+ +C++C   +  DYKNV+LLSQFISP +G  Y  HITGLC     +I   I RA+  G M    K+  Y
Sbjct:   26 RGTQAVSVIWRRCFSQFEQVTSNEDLPV-PMENPYKEPLKKCVLCEKRV--DYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQKMGFMPVTYKDPAY 130
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Match: sp|P82917|RT18C_BOVIN (28S ribosomal protein S18c, mitochondrial OS=Bos taurus OX=9913 GN=MRPS18C PE=1 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 1.108e-17
Identity = 39/90 (43.33%), Postives = 54/90 (60.00%), Query Frame = 1 
Query:   88 ETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            +     DLP+  M++P+K+P+ +CI+C   +  DYKNV+LLSQFISP +G  Y  HITGLC     +I   I RA+  G M    K+  Y
Sbjct:   44 QVTSSEDLPI-PMENPYKEPLKKCILCEKRV--DYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQILGFMPVTYKDPAY 130
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Match: sp|Q99N84|RT18B_MOUSE (28S ribosomal protein S18b, mitochondrial OS=Mus musculus OX=10090 GN=Mrps18b PE=1 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 3.295e-7
Identity = 23/67 (34.33%), Postives = 43/67 (64.18%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            N C +C +  + +D++NV+LL QF+   +G  + A  TG+C   H+K+   I +AR+CGL+++ + +
Sbjct:  101 NPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARECGLLSYYVPQ 167
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Match: sp|P82918|RT18B_BOVIN (28S ribosomal protein S18b, mitochondrial OS=Bos taurus OX=9913 GN=MRPS18B PE=1 SV=2)

HSP 1 Score: 49.6766 bits (117), Expect = 1.018e-6
Identity = 24/67 (35.82%), Postives = 42/67 (62.69%), Query Frame = 1 
Query:  151 NRCIVCVND-IPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKE 348
            N C +C +  + +D++NV+LL QF+   +G  + A  TG+C   H+K+   I +ARD GL+++ I +
Sbjct:  103 NPCPICRDQKLHVDFRNVKLLKQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARDHGLLSYHIPQ 169
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Match: A0A5K3FHT4 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.047e-27
Identity = 51/110 (46.36%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:   28 LFRSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            L RS  +F+ + +  R  +S  +  ND+P+ ++++P+K    +C +C N+I LDYKNV+LLSQF+SP +GR Y  HITGLC  M +++  LI RAR  G MAHE+KET++
Sbjct:    5 LPRSSLMFSPLVRCTRLVSSAASQSNDMPI-HIENPYKPTKRQCFICKNNIELDYKNVQLLSQFVSPYTGRVYGRHITGLCIPMQKRVATLIERARKFGFMAHEMKETIF 113
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Match: A0A267EB88 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024874g1 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.447e-27
Identity = 44/114 (38.60%), Postives = 71/114 (62.28%), Query Frame = 1 
Query:   28 LFRSKFIFNVIKKNVRNFTSET----NIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            + R + +   + +     ++        + D+P+ +M DP+KKP+ +C++C  D+PLDYKN RLLSQF+SP +GR Y  HITGLC  M E++  L+ +++  G M+  IKET +
Sbjct:    1 MLRCRLVLTAVGRGKLGLSTGAAPAGQTDPDMPLADMPDPYKKPIKKCVLCRQDVPLDYKNARLLSQFVSPYTGRIYGRHITGLCQPMQERVAELVKKSQKFGYMSQNIKETFF 114
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Match: A0A267DXB5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024874g2 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.253e-27
Identity = 44/112 (39.29%), Postives = 70/112 (62.50%), Query Frame = 1 
Query:   34 RSKFIFNVIKKNVRNFTSETNIEN----DLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            R + +   + +     ++   +      D+P+ +M DP+KKP+ +C++C  D+PLDYKN RLLSQF+SP +GR Y  HITGLC  M E++  L+ +++  G M+  IKET +
Sbjct:    1 RCRLVLTAVGRGKLGLSTGAALAGQTDPDMPLADMPDPYKKPIKKCVLCRQDVPLDYKNARLLSQFVSPYTGRIYGRHITGLCQPMQERVAELVKKSQKFGYMSQNIKETFF 112
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Match: A0A267GE83 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024874g3 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.254e-27
Identity = 43/88 (48.86%), Postives = 63/88 (71.59%), Query Frame = 1 
Query:   94 NIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
              + D+P+ +M DP+KKP+ +C++C  D+PLDYKN RLLSQF+SP +GR Y  HITGLC  M E++  L+ +++  G M+  IKET +
Sbjct:   24 QTDPDMPLADMPDPYKKPIKKCVLCRQDVPLDYKNARLLSQFVSPYTGRIYGRHITGLCQPMQERVAELVKKSQKFGYMSQNIKETFF 111
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Match: C1LJJ7 (28S ribosomal protein S18c isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_002640 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.858e-25
Identity = 49/97 (50.52%), Postives = 67/97 (69.07%), Query Frame = 1 
Query:   67 NVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            +V+  T + + END P+ +M DP+ KP  +C +C  +IPLDYKNVRLLSQF+SP +GR Y  HITG+C  M ++I  LI R+R  G M  E KET++
Sbjct:   34 SVQFSTGKYDSENDKPI-DMPDPYLKPKKKCFICEKNIPLDYKNVRLLSQFVSPYTGRIYGRHITGMCIPMQKRISQLITRSRQFGFMPFESKETVF 129
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Cavefish
Match: mrps18c (mitochondrial ribosomal protein S18C [Source:ZFIN;Acc:ZDB-GENE-060503-609])

HSP 1 Score: 78.5666 bits (192), Expect = 1.174e-19
Identity = 36/78 (46.15%), Postives = 52/78 (66.67%), Query Frame = 1 
Query:  124 MDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            MD+P+K+P   CI+C  ++ +D+KNV+LLSQFISP +GR Y  HITGLC     ++   I RAR  G M+  +K+  +
Sbjct:   11 MDNPYKQPQKGCILC--NVSVDFKNVQLLSQFISPHTGRIYGRHITGLCGQKQREVTKAIKRARSLGFMSVTLKDPQF 86
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Cavefish
Match: mrps18b (mitochondrial ribosomal protein S18B [Source:NCBI gene;Acc:103044234])

HSP 1 Score: 45.4394 bits (106), Expect = 3.260e-6
Identity = 24/65 (36.92%), Postives = 38/65 (58.46%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C +C + +I + Y+NV LL QFISP +G  YD   TG+C    +++   I  A+  GL+  ++
Sbjct:  124 NPCPICRDQNIIIHYQNVSLLQQFISPYTGFVYDPTRTGVCMRQQKRLNTAIETAKLHGLIPTQL 188
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Sea Lamprey
Match: mrps18b (mitochondrial ribosomal protein S18B [Source:ZFIN;Acc:ZDB-GENE-050417-278])

HSP 1 Score: 50.447 bits (119), Expect = 1.117e-8
Identity = 25/59 (42.37%), Postives = 35/59 (59.32%), Query Frame = 1 
Query:  151 NRCIVCVND-IPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCG 324
            N C  C +  I +D+KN+RLL QFI P  G  +D  +TGLC   H+ +   I +A+D G
Sbjct:  104 NPCPACRDPKIIMDFKNIRLLQQFICPHQGIVFDPSLTGLCQRQHKNLLAAIKKAQDYG 162
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Nematostella
Match: EDO47606 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLH4])

HSP 1 Score: 63.929 bits (154), Expect = 6.774e-14
Identity = 33/65 (50.77%), Postives = 43/65 (66.15%), Query Frame = 1 
Query:  157 CIVCV--NDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIK 345
            C++C    D+ +DYKNVRLLSQFIS  SGR Y+  +TGLC+   ++I   I RAR  GLM   +K
Sbjct:   83 CLLCPKRTDVTIDYKNVRLLSQFISSHSGRIYNRRVTGLCDKKQKEITNAIKRARAIGLMPVTMK 147
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Medaka
Match: mrps18b (mitochondrial ribosomal protein S18B [Source:NCBI gene;Acc:101172345])

HSP 1 Score: 44.669 bits (104), Expect = 5.774e-6
Identity = 24/65 (36.92%), Postives = 38/65 (58.46%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C VC + ++ +  +NV+LL QF+SP +G  YD   TG+C    +K+   I  A+D G +  +I
Sbjct:  101 NPCPVCRDSNLIVHPQNVKLLQQFMSPHTGAVYDPTRTGVCMKQQKKLNEAINTAQDQGTLPFQI 165
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Medaka
Match: mrps18b (mitochondrial ribosomal protein S18B [Source:NCBI gene;Acc:101172345])

HSP 1 Score: 44.669 bits (104), Expect = 6.320e-6
Identity = 24/65 (36.92%), Postives = 38/65 (58.46%), Query Frame = 1 
Query:  151 NRCIVCVN-DIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEI 342
            N C VC + ++ +  +NV+LL QF+SP +G  YD   TG+C    +K+   I  A+D G +  +I
Sbjct:  116 NPCPVCRDSNLIVHPQNVKLLQQFMSPHTGAVYDPTRTGVCMKQQKKLNEAINTAQDQGTLPFQI 180
[Close]
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Planmine SMEST
Match: SMESG000018393.1 (SMESG000018393.1)

HSP 1 Score: 233.032 bits (593), Expect = 1.980e-80
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1 
Query:   22 MNLFRSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 357
            MNLFRSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY
Sbjct:    1 MNLFRSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPMNRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKILIGRARDCGLMAHEIKETLY 112
The following BLAST results are available for this feature:
BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MRPS18C3.318e-1941.05mitochondrial ribosomal protein S18C [Source:HGNC ... [more]
MRPS18C2.236e-645.10mitochondrial ribosomal protein S18C [Source:HGNC ... [more]
MRPS18C2.290e-645.10mitochondrial ribosomal protein S18C [Source:HGNC ... [more]
MRPS18B3.784e-634.33mitochondrial ribosomal protein S18B [Source:HGNC ... [more]
MRPS18B3.784e-634.33mitochondrial ribosomal protein S18B [Source:HGNC ... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps-18.C5.370e-1942.35Mitochondrial Ribosomal Protein, Small [Source:Un... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mRpS18C2.756e-1748.00gene:FBgn0039765 transcript:FBtr0085625[more]
mRpS18B1.128e-938.46gene:FBgn0032849 transcript:FBtr0081338[more]
mRpS18B1.128e-938.46gene:FBgn0032849 transcript:FBtr0081339[more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mrps18c7.551e-2137.17mitochondrial ribosomal protein S18C [Source:ZFIN;... [more]
mrps18c7.551e-2137.17mitochondrial ribosomal protein S18C [Source:ZFIN;... [more]
mrps18b6.879e-638.46mitochondrial ribosomal protein S18B [Source:ZFIN;... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps18c2.988e-1538.64mitochondrial ribosomal protein S18C [Source:NCBI ... [more]
ENSXETT00000017678.17.862e-736.92mitochondrial ribosomal protein S18B [Source:NCBI ... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Mrps18c8.863e-1938.89mitochondrial ribosomal protein S18C [Source:MGI S... [more]
Mrps18c2.147e-1748.65mitochondrial ribosomal protein S18C [Source:MGI S... [more]
Mrps18b4.711e-834.33mitochondrial ribosomal protein S18B [Source:MGI S... [more]
Mrps18b3.003e-636.54mitochondrial ribosomal protein S18B [Source:MGI S... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9Y3D5|RT18C_HUMAN1.593e-1841.0528S ribosomal protein S18c, mitochondrial OS=Homo ... [more]
sp|Q8R2L5|RT18C_MOUSE6.200e-1838.8928S ribosomal protein S18c, mitochondrial OS=Mus m... [more]
sp|P82917|RT18C_BOVIN1.108e-1743.3328S ribosomal protein S18c, mitochondrial OS=Bos t... [more]
sp|Q99N84|RT18B_MOUSE3.295e-734.3328S ribosomal protein S18b, mitochondrial OS=Mus m... [more]
sp|P82918|RT18B_BOVIN1.018e-635.8228S ribosomal protein S18b, mitochondrial OS=Bos t... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3FHT41.047e-2746.36Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A267EB882.447e-2738.60Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DXB54.253e-2739.29Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267GE835.254e-2748.86Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
C1LJJ71.858e-2550.5228S ribosomal protein S18c isoform 1 OS=Schistosom... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps18c1.174e-1946.15mitochondrial ribosomal protein S18C [Source:ZFIN;... [more]
mrps18b3.260e-636.92mitochondrial ribosomal protein S18B [Source:NCBI ... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps18b1.117e-842.37mitochondrial ribosomal protein S18B [Source:ZFIN;... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO476066.774e-1450.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps18b5.774e-636.92mitochondrial ribosomal protein S18B [Source:NCBI ... [more]
mrps18b6.320e-636.92mitochondrial ribosomal protein S18B [Source:NCBI ... [more]
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BLAST of 28S ribosomal protein S18c, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000018393.11.980e-80100.00SMESG000018393.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008248 ID=SMED30008248|Name=28S ribosomal protein S18c, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=416bp
ATTTGTCATATTTCAAAAACAATGAATCTATTTCGAAGCAAATTTATTTT
CAATGTTATTAAAAAAAACGTCAGAAATTTTACTTCAGAAACTAATATTG
AGAATGATTTGCCTGTATTTAATATGGACGATCCTTTTAAAAAGCCCATG
AATAGATGCATAGTTTGTGTGAATGATATTCCTTTGGATTATAAAAATGT
TCGATTGCTGTCACAATTTATTTCTCCAATGTCTGGACGACAATATGATG
CTCATATAACTGGGTTATGCAATAATATGCATGAAAAAATCAAAATTTTA
ATTGGAAGGGCCCGAGATTGTGGTTTAATGGCACATGAAATTAAAGAAAC
TTTATATATAGACGATATTAAAATAATCTCAAAAAATATAAGTTAATAAA
TATATTGCTGCAATGA
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protein sequence of SMED30008248-orf-1

>SMED30008248-orf-1 ID=SMED30008248-orf-1|Name=SMED30008248-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
ICHISKTMNLFRSKFIFNVIKKNVRNFTSETNIENDLPVFNMDDPFKKPM
NRCIVCVNDIPLDYKNVRLLSQFISPMSGRQYDAHITGLCNNMHEKIKIL
IGRARDCGLMAHEIKETLYIDDIKIISKNIS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036870Ribosomal_S18_sf
IPR001648Ribosomal_S18
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001648Ribosomal protein S18PFAMPF01084Ribosomal_S18coord: 60..110
e-value: 4.0E-14
score: 52.5
IPR036870Ribosomal protein S18 superfamilyGENE3DG3DSA:4.10.640.10coord: 53..116
e-value: 3.3E-10
score: 41.4
IPR036870Ribosomal protein S18 superfamilySUPERFAMILYSSF46911Ribosomal protein S18coord: 46..113
NoneNo IPR availablePANTHERPTHR1347930S RIBOSOMAL PROTEIN S18coord: 32..126
NoneNo IPR availablePANTHERPTHR13479:SF5728S RIBOSOMAL PROTEIN S18C, MITOCHONDRIALcoord: 32..126
NoneNo IPR availablePRODOMPD002239coord: 63..110
e-value: 1.0E-9
score: 145.0