SMED30008241
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008241 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11Note: Hover over icons to view figure legend
Alignments
SMED30008241 aligns in the following genomic locations:
Homology
BLAST of SMED30008241 vs. Planmine SMEST
Match: SMESG000005752.1 (SMESG000005752.1) HSP 1 Score: 245.358 bits (625), Expect = 1.691e-74 Identity = 197/209 (94.26%), Postives = 202/209 (96.65%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESV 629 EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKE+SIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSEN+EIVDEHE +TC EE YE L +K+E V Sbjct: 7534 EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEDSIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENIEIVDEHEAKTCGEELYEKSLTSKVEDV 7742 HSP 2 Score: 236.113 bits (601), Expect = 2.271e-71 Identity = 192/220 (87.27%), Postives = 211/220 (95.91%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQ 662 EHE +TC+EE Y+ PLE+K+E+V ET++IE+YPQ+LEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDK FETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEESIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENVEIVDEHE +TC+EE Y+ PLE+K+E+V ET++IE+YPQ Sbjct: 7348 EHETETCKEEFYEAPLENKMESVIETELIEEYPQSLEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKVFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEESIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENVEIVDEHEAKTCEEELYKKPLESKVENVFETKVIEQYPQ 7567 HSP 3 Score: 122.865 bits (307), Expect = 6.288e-32 Identity = 110/221 (49.77%), Postives = 139/221 (62.90%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVEN-VFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQ 662 E EA ++ L LE+K+EN V ET+VI++ + LE ++I EEE+N++E +KP E L E +E+V++ EI D EIKQ ++ T V EN E + + K++KPEIN+ E I+ E+ + E+ NVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQ Sbjct: 7185 EQEANIYQDLLDVVSLENKIENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKP--EELTTVLISESVEKVDESEIID-IAEIKQTDSTTTVVENFLEIQVIDEKADKPEINILTETNIQSEVEMETALEH---------------------TENVNVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQ 7381 HSP 4 Score: 103.99 bits (258), Expect = 2.137e-25 Identity = 110/210 (52.38%), Postives = 150/210 (71.43%), Query Frame = 3 Query: 48 LESKVE-NVFETKVIEQYPQALEA---SNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKM-ESVIETELIEEYPQ 662 LESK E NVF+T+VIE++PQ +EA N+ED V N++ LSE VEQ ++++++E+K+E EIK T++ +N EDK E KSE PE+NV EE+K E E+V+ E+V +E IEK+ESIE SFEIE+ELSD+E E V+ADF+TKDN+ELQ++SEN EI +EHETE CKEE YE LE+KM E+ +++IEE+PQ Sbjct: 8776 LESKAEENVFKTQVIEEHPQFVEALITDNIEDEVV--------------NDVAVLSEGVEQTQKLDENEVKEEIPEIKPITALTSLIKNILEDKVCEHKSEMPEVNVLEEVKTEMEVVETEDVIKEIIEKQESIEGSFEIEMELSDIEREIVKADFETKDNKELQVLSENAEIQEEHETEKCKEELYEVNLESKMEENASMSKVIEEHPQ 8971 HSP 5 Score: 78.1814 bits (191), Expect = 1.556e-16 Identity = 97/222 (43.69%), Postives = 138/222 (62.16%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVE-NVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKM-ESVIETELIEEYPQ 662 E +A+T E + + L S+VE N+FET+VIE+ PQ LE + + DIVEEE ++E TI+KP+ ++ LSEA+EQ + ++Q EI +E EIK V +N+ E+ K E E +++ V EV + ES+EDSFE+E+EL DVE NVEA+FKTKD+E+ +IVS +E VDEHE E KE E LENK+ E+V + + IE+ P Sbjct: 8051 EQKAETSGETMDEVCLASRVEQNLFETQVIEEIPQFLETTIISDIVEEETKSVEGTIVKPEVKMEILSEAIEQTQNLKQSEI-NEISEIKPNAASVLVIDNSPEENVLGLKPET-FDVPILEESNKQDEVTEMEVKGNIVVNAESLEDSFEMEMELLDVESANVEAEFKTKDSEDFKIVSGCIETVDEHEAEAFKEVLNEVSLENKIEENVFQIKAIEKQPH 8270 HSP 6 Score: 72.7886 bits (177), Expect = 1.381e-14 Identity = 81/221 (36.65%), Postives = 109/221 (49.32%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKME-SVIETELIEEYPQ 662 EHEAKTC EELY+K L SKVE+V +I IKP + LSE+VEQIE+VEQ EIKDE E+ Q++ F AV EN + K E K+ KP + E ++ E + E E EN A KT+ N +LQ VSE++E +++H T +E E LEN E +V +TE+I+E+PQ Sbjct: 7720 EHEAKTCGEELYEKSLTSKVEDVSTEAII---------------------------IKPDDMKNILSESVEQIEKVEQCEIKDEFAEVMQDKAFEAVLENVLDVKATEPKTTKP---------ERSILEIANVQVEIMKDESFMEESIEKEESLQVSFESENEYA--KTESNVDLQNVSEDIENLEQHATNIYEERLDEVQLENVTELNVFKTEIIQEHPQ 7902 HSP 7 Score: 67.3958 bits (163), Expect = 9.601e-13 Identity = 42/65 (64.62%), Postives = 55/65 (84.62%), Query Frame = 3 Query: 471 DVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKM-ESVIETELIEEYPQ 662 DVE+E+VEA+FKTKDN+ELQI+SENVE DEH+TET +EF E LE+K E+V +T++IEE+PQ Sbjct: 8732 DVENEDVEAEFKTKDNKELQILSENVENHDEHQTETF-QEFDEVNLESKAEENVFKTQVIEEHPQ 8795 HSP 8 Score: 66.2402 bits (160), Expect = 2.099e-12 Identity = 99/222 (44.59%), Postives = 141/222 (63.51%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVE-NVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKME-SVIETELIEEYPQ 662 +H EE L + LE+ E NVF+T++I+++PQ LE+ + + EE+ +EA I+ PK + LSEAVEQIE +EQ EI+D+ I+Q+ V N E + ETK E+ + + E KIE E E EETIEKEESI+DSFEIE+ELS+V++E+V+ +FK KD E SEN+EIV E + ET E E L +++E ++ ET++IEE PQ Sbjct: 7868 QHATNIYEERLDEVQLENVTELNVFKTEIIQEHPQFLESLTNDTMAGEEMRILEAIIVAPKEVIKVLSEAVEQIETIEQSEIQDDLANIEQD----IVLANVIEAQVLETKLEQADEYISMETKIEIETPNPEATTEETIEKEESIDDSFEIEMELSEVKNEDVKEEFKMKDKVEHHGASENIEIVHEQKAETSGETMDEVCLASRVEQNLFETQVIEEIPQ 8085 HSP 9 Score: 62.003 bits (149), Expect = 5.961e-11 Identity = 36/64 (56.25%), Postives = 49/64 (76.56%), Query Frame = 3 Query: 471 DVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKM-ESVIETELIEEYP 659 D+E+E+VEADFKTKDN+ELQ VSEN+EI DEHE E E +++K+ E+V +TE+I+E P Sbjct: 8564 DLENEDVEADFKTKDNKELQNVSENLEITDEHEANIHDEALEEVSIQHKLEENVFKTEIIKEQP 8627 HSP 10 Score: 57.3806 bits (137), Expect = 1.900e-9 Identity = 96/222 (43.24%), Postives = 135/222 (60.81%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVE-NVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVEHENVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKME-SVIETELIEEYPQ 662 EHEA+ +E L + LE+K+E NVF+ K IE+ P LE E+I EEEI TI+A ++ + LSEAVEQ ++ EQ EI + EI+Q +V E + E +SEKP + +++ ++ E EKEESIEDSFEIEIELSD E++ +EADFK K++ LQ VSENVE + ++ +EE E LEN++E +V +T I E+PQ Sbjct: 8236 EHEAEAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKP----------KLNLLEESKIETEITEKEESIEDSFEIEIELSDAENQELEADFKAKESLALQNVSENVENICDYAGHLSEEELNEVYLENEVEDNVYDTIAIVEHPQ 8447 HSP 11 Score: 55.4546 bits (132), Expect = 9.265e-9 Identity = 57/115 (49.57%), Postives = 80/115 (69.57%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVE-NVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKF--FETKSE 338 EHE + C+EELY+ LESK+E N +KVIE++PQ LE E+IVEEEINT+EATI++ + +L LSE +EQIE++E+D IKD LE ++++ + + E K FETK E Sbjct: 8937 EHETEKCKEELYEVNLESKMEENASMSKVIEEHPQILEKEINENIVEEEINTVEATIVRSEEQLPVLSEVIEQIEKLEKDVIKDIHLETEEDKAVLTMVDAMEETKAESFETKIE 9051 HSP 12 Score: 49.2914 bits (116), Expect = 1.232e-6 Identity = 49/106 (46.23%), Postives = 73/106 (68.87%), Query Frame = 3 Query: 3 EHEAKTCEEELYKKPLESKVE-NVFETKVIEQYPQALEASNVEDXXXXXXXXXXXXXXKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDK 317 ++ EEEL + LE++VE NV++T I ++PQ LEA E+I+E+EINTIEATI+K + +L LSEAVEQ ++EQ + ++ LEI + TA+ EN+ E+K Sbjct: 8413 DYAGHLSEEELNEVYLENEVEDNVYDTIAIVEHPQTLEAMATENIIEQEINTIEATIVKTEKKLSTLSEAVEQNVKIEQGGVNEDILEIIETRASTALIENSLENK 8518 The following BLAST results are available for this feature:
BLAST of SMED30008241 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30008241 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30008241 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30008241 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30008241 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008241 ID=SMED30008241|Name=SMED30008241|organism=Schmidtea mediterranea sexual|type=transcript|length=663bpback to top protein sequence of SMED30008241-orf-1 >SMED30008241-orf-1 ID=SMED30008241-orf-1|Name=SMED30008241-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=220bp EHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDIVEEEINback to top Annotated Terms
The following terms have been associated with this transcript:
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