SMED30008236

Overview
NameSMED30008236
Smed IDSMED30008236
Length (bp)827
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30008236 (SMED30008236) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30008236 (SMED30008236) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30008236 (SMED30008236) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30008236 (SMED30008236) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008236

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30008236h1SMcG0008814 dd_Smed_v4_28015_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008236h1SMcG0008814 dd_Smed_v4_28015_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30008236h1SMcG0008814 dd_Smed_v4_28015_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30008236h1SMcG0008814 dd_28015dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
neuronSMED30008236h1SMcG0008814 dd_28015dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30008236 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005024:30529..31355 -4099
v31.036328:1..672 +3288
Homology
[Close]
BLAST of SMED30008236 vs. TrEMBL
Match: A0A1I8GLP2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 9.093e-9
Identity = 44/124 (35.48%), Postives = 67/124 (54.03%), Query Frame = 1 
Query:  301 FRSVILDEWS-ALRPSGMNVTIRAVNNA--IVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSA 651
            +RS +LD    A++ SG    +  +     +V  + F VS      N  +WFT   ++ SFP++   LK + + FFS  G +   R+FFI+ RY GC GDVG  ++ D +D CS++ K W  SA
Sbjct:  900 YRSKLLDTLPFAVKRSGYGKIVAEMRTGSKVVAQVVFDVSTNTKKLNFQNWFTRTRMVSSFPYE---LKKTGFNFFSFAGHQAHKRTFFINNRYRGCGGDVGFWVVSDRKDVCSWDNKGWHGSA 1020

HSP 2 Score: 66.6254 bits (161), Expect = 1.456e-8
Identity = 37/107 (34.58%), Postives = 61/107 (57.01%), Query Frame = 1 
Query:  382 IVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNG 690
            +V  L F VS      N+++WF+   L+ SFP++I     + + +FS+ G +   R+FF++  Y GC GD G  M+ D +D CS+  K W  SA   P++ Y+ ++G
Sbjct:  725 VVAQLVFDVSTNKKTLNLMNWFSPTRLVSSFPYEIG---KTGFNYFSMAGHKALKRTFFVNYVYRGCPGDTGFWMIADKKDVCSWAKKGWKGSA---PELVYSQVSG 825

HSP 3 Score: 65.0846 bits (157), Expect = 5.002e-8
Identity = 34/93 (36.56%), Postives = 52/93 (55.91%), Query Frame = 1 
Query:  421 NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNGSP 696
            N  +WFT   ++ SFP+    LK + + +FS  G +   R+FFIS RY GC GD+G  ++ D +D C ++ K W +S    P + Y  + G P
Sbjct: 1151 NFQNWFTPARMVSSFPYK---LKKTGFNYFSFAGHQAHKRTFFISNRYRGCGGDIGFWVISDRKDVCKWSNKGWHSSG---PVLVYTQVPGVP 1237

HSP 4 Score: 62.003 bits (149), Expect = 4.493e-7
Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 1 
Query:  424 IISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSY-NLKWITSA 651
            + +WFT   L+ S+P+    L  + Y +FSV G R   RSFFI+  Y GC GD+G  M+ D RD C + N  W  SA
Sbjct: 1542 MTNWFTPTRLVSSYPYH---LGKTGYNYFSVAGHRAHKRSFFINKVYAGCAGDLGFWMVSDRRDPCKWANKGWSGSA 1615
[Close]
BLAST of SMED30008236 vs. TrEMBL
Match: A0A1I8GIN4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.425e-8
Identity = 39/115 (33.91%), Postives = 63/115 (54.78%), Query Frame = 1 
Query:  373 NNAIVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNGSPAIF 705
            +  +V  L F VS      N+++WF+   L+ SFP++I     + + +FS+ G +   R+FF++  Y GC GD G  M+ D +D CS+  K W  SA   P++ Y+VL  S   +
Sbjct:  288 HGRVVAQLVFDVSTNKKTLNLMNWFSPTRLVSSFPYEIG---KTGFNYFSMAGHKALKRTFFVNYVYRGCPGDTGFWMIADKKDVCSWAKKGWKGSA---PELVYSVLKYSQPFY 396

HSP 2 Score: 63.929 bits (154), Expect = 1.062e-7
Identity = 46/139 (33.09%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:  301 FRSVILDEWS-ALRPSGMN--VTIRAVNNAIVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSY-NLKWITSAINYPQIFYAVLNGSP 696
            +RS  LD  + A+  SG    V      + +V  + F VS      N  +WFT   ++ SFP+    LK + + +FS  G +   R+FFIS RY GC GD+G  ++ D +D C + N  W +S    P + Y  + G P
Sbjct:  564 YRSDQLDTLANAVERSGFKHIVAEMRAGSRVVAQVVFDVSINTKSLNFQNWFTPARMVSSFPYK---LKKTGFNYFSFAGHQAHKRTFFISNRYRGCGGDIGFWVISDRKDVCKWSNKGWHSSG---PVLVYTQVPGVP 696

HSP 3 Score: 58.5362 bits (140), Expect = 5.832e-6
Identity = 35/92 (38.04%), Postives = 51/92 (55.43%), Query Frame = 1 
Query:  421 NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYN-GCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNG 690
            N+++WF+   L+ S+P+    L  S Y +FSV G +   R+FFI+  Y  GC GD+G  ++ D RD C +  K W  SA   P + Y  L G
Sbjct:  815 NMVNWFSKSRLVSSYPYQ---LGKSGYNYFSVAGHQAYKRTFFINSVYKAGCAGDIGFWVVSDRRDPCKWAYKGWHGSA---PALIYTRLPG 900
[Close]
BLAST of SMED30008236 vs. TrEMBL
Match: A0A1I8GLC5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.525e-8
Identity = 44/124 (35.48%), Postives = 67/124 (54.03%), Query Frame = 1 
Query:  301 FRSVILDEWS-ALRPSGMNVTIRAVNNA--IVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSA 651
            +RS +LD    A++ SG    +  +     +V  + F VS      N  +WFT   ++ SFP++   LK + + FFS  G +   R+FFI+ RY GC GDVG  ++ D +D CS++ K W  SA
Sbjct:  466 YRSKLLDTLPFAVKRSGYGKIVAEMRTGSKVVAQVVFDVSTNTKKLNFQNWFTRTRMVSSFPYE---LKKTGFNFFSFAGHQAHKRTFFINNRYRGCGGDVGFWVVSDRKDVCSWDNKGWHGSA 586

HSP 2 Score: 65.0846 bits (157), Expect = 5.101e-8
Identity = 37/110 (33.64%), Postives = 62/110 (56.36%), Query Frame = 1 
Query:  373 NNAIVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNG 690
            +  +V  L F VS      N+++WF+   L+ SFP++I     + + +FS+ G +   R+FF++  Y GC GD G  M+ D +D CS+  K W  SA   P++ Y+ ++G
Sbjct:  288 HGRVVAQLVFDVSTNKKTLNLMNWFSPTRLVSSFPYEIG---KTGFNYFSMAGHKALKRTFFVNYVYRGCPGDTGFWMIADKKDVCSWAKKGWKGSA---PELVYSQVSG 391

HSP 3 Score: 63.5438 bits (153), Expect = 1.456e-7
Identity = 46/139 (33.09%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:  301 FRSVILDEWS-ALRPSGMN--VTIRAVNNAIVGWLNFRVSAQD---NIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSY-NLKWITSAINYPQIFYAVLNGSP 696
            +RS  LD  + A+  SG    V      + +V  + F VS      N  +WFT   ++ SFP+    LK + + +FS  G +   R+FFIS RY GC GD+G  ++ D +D C + N  W +S    P + Y  + G P
Sbjct:  671 YRSDQLDTLANAVERSGFKHIVAEMRAGSRVVAQVVFDVSINTKSLNFQNWFTPARMVSSFPYK---LKKTGFNYFSFAGHQAHKRTFFISNRYRGCGGDIGFWVISDRKDVCKWSNKGWHSSG---PVLVYTQVPGVP 803

HSP 4 Score: 60.4622 bits (145), Expect = 1.441e-6
Identity = 33/84 (39.29%), Postives = 46/84 (54.76%), Query Frame = 1 
Query:  403 RVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSY-NLKWITSA 651
            R +    + +WFT   L+ S+P+    L  + Y +FSV G R   R+FFI+  Y GC GD+G  M+ D RD C + N  W  SA
Sbjct: 1089 RHTKSVGMTNWFTPTRLVSSYPYH---LGKTGYNYFSVAGHRAHKRTFFINKVYAGCAGDLGFWMVSDRRDPCKWANKGWSGSA 1169
[Close]
BLAST of SMED30008236 vs. TrEMBL
Match: A0A0L8H6T8 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22021012mg PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.631e-8
Identity = 53/197 (26.90%), Postives = 88/197 (44.67%), Query Frame = 1 
Query:  106 YIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENF-RSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNII-SWFTLGNLLDSFPW-DINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLN 687
            Y PK +    + +   +I + R    I V  +  G+G    D  +   +PS++ V+     +  NF R  ILD WS L      + I     A+V   N     ++  + SWF+L  L  S PW D++    ++++ F        +R F++S  Y GC GD G +++ + R  C Y         +YPQ+ Y+  N
Sbjct:   89 YSPKNLMTVVSTNDSNEISFYRNTQWIKVYALSMGAGSKVYDSFANKGSPSSWNVEKCIGTFCPNFFRHPILDVWSHLPIDEAKLVIYENQTAVV---NMVFDGRNTTLESWFSLETLKSS-PWPDLHQQPVNIFSLFGA-----AIRRFYVSSEYGGCVGDWGWMLIAEARHMCYYE-----QLSSYPQLLYSKRN 271
[Close]
BLAST of SMED30008236 vs. TrEMBL
Match: A0A1I8G660 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.844e-8
Identity = 43/140 (30.71%), Postives = 73/140 (52.14%), Query Frame = 1 
Query:  301 FRSVILDEW-SALRPSGMN---VTIRAVNNAIVGWLNFRVSAQDNII---SWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLK-WITSAINYPQIFYAVLNGSP 696
            +RS ++DE   A+R SGM    V +R  +N +V  + F VS     +   +WF+   L  S+P+ ++      + +FS+ G+R   R F+I+  ++GC  + G +++ D R  C Y  + W  SA   P + Y+ L G P
Sbjct:  474 YRSDLIDELPRAVRQSGMTKMVVEMRKDDNQVVSQVVFDVSTDTEKLDKENWFSELRLESSYPYSVD---RKEFNYFSLEGERSSKRRFYINNWHHGCHRETGFILVSDARGHCDYVTRGWRGSA---PTLIYSRLPGKP 607

HSP 2 Score: 63.1586 bits (152), Expect = 2.169e-7
Identity = 49/164 (29.88%), Postives = 79/164 (48.17%), Query Frame = 1 
Query:  301 FRSVILDEW-SALRPSGMNVTIRAV--NNAIVGWLNFRVSAQDNII---SWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            +RS ++DE   A+R SGM   +  +  +N +V  + F VS   + +   +WF+L  L  S+P+ I+      + +FS+ GD    R F+IS  Y GCT D G +M+ D R +C     W+T                P++ YN  +     E +G Y   ++IY
Sbjct:  884 YRSDLIDELPRAVRQSGMTKMVVEMRKDNKVVSQVAFDVSTDTDKLDKENWFSLLRLYSSYPYPIS---RKEFNYFSLEGD--ASRKFYISNHYGGCTRDSGFIMVSDSRGSCD----WVTRGWR---------GSPPSLAYNREQGRSFHESVG-YADRLIIY 1028

HSP 3 Score: 63.1586 bits (152), Expect = 2.169e-7
Identity = 49/164 (29.88%), Postives = 79/164 (48.17%), Query Frame = 1 
Query:  301 FRSVILDEW-SALRPSGMNVTIRAV--NNAIVGWLNFRVSAQDNII---SWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            +RS ++DE   A+R SGM   +  +  +N +V  + F VS   + +   +WF+L  L  S+P+ I+      + +FS+ GD    R F+IS  Y GCT D G +M+ D R +C     W+T                P++ YN  +     E +G Y   ++IY
Sbjct: 1291 YRSDLIDELPRAVRQSGMTKMVVEMRKDNKVVSQVAFDVSTDTDKLDKENWFSLLRLYSSYPYPIS---RKEFNYFSLEGD--ASRKFYISNHYGGCTRDSGFIMVSDSRGSCD----WVTRGWR---------GSPPSLAYNREQGRSFHESVG-YADRLIIY 1435

HSP 4 Score: 63.1586 bits (152), Expect = 2.169e-7
Identity = 49/164 (29.88%), Postives = 79/164 (48.17%), Query Frame = 1 
Query:  301 FRSVILDEW-SALRPSGMNVTIRAV--NNAIVGWLNFRVSAQDNII---SWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            +RS ++DE   A+R SGM   +  +  +N +V  + F VS   + +   +WF+L  L  S+P+ I+      + +FS+ GD    R F+IS  Y GCT D G +M+ D R +C     W+T                P++ YN  +     E +G Y   ++IY
Sbjct: 1698 YRSDLIDELPRAVRQSGMTKMVVEMRKDNKVVSQVAFDVSTDTDKLDKENWFSLLRLYSSYPYPIS---RKEFNYFSLEGD--ASRKFYISNHYGGCTRDSGFIMVSDSRGSCD----WVTRGWR---------GSPPSLAYNREQGRSFHESVG-YADRLIIY 1842
[Close]
BLAST of SMED30008236 vs. Planmine SMEST
Match: SMESG000076267.1 (SMESG000076267.1)

HSP 1 Score: 518.079 bits (1333), Expect = 0.000e+0
Identity = 254/259 (98.07%), Postives = 254/259 (98.07%), Query Frame = 1 
Query:    1 VWFSGKKGDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIYG 777
            VWFSGKKGDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWS LRPSGMNVTIRAVN AIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLY FFSVTGDRKGLRSFFISIRYNGCTGD GVLMMFDG DACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIYG
Sbjct:   11 VWFSGKKGDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSTLRPSGMNVTIRAVNKAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYKFFSVTGDRKGLRSFFISIRYNGCTGDAGVLMMFDGSDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIYG 269
[Close]
BLAST of SMED30008236 vs. Planmine SMEST
Match: SMESG000076443.1 (SMESG000076443.1)

HSP 1 Score: 282.722 bits (722), Expect = 2.132e-95
Identity = 138/271 (50.92%), Postives = 186/271 (68.63%), Query Frame = 1 
Query:   22 GDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSPT--------------------KIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            GD ++   C L+ F + + YF++V+  C+ P     QPTQ + +                    K +Y+++ NT+ +LRIVNGSGVYPPD+L+  +NPS Y VKD + N+SENFRS++LD+W   +PS +NVTIR V++  VGWL F V  +DNI+SWF   NL+DSFPW+INVLKNS Y +FS+ GD+   RSF ++ +Y GC  DVGVLMMFDG + C+Y L W+TSA  YPQI YA LNGSP+ + NY+R AKSLEIIG+YPS+I I+
Sbjct:   17 GDSVEFADCILQTFQKKLTYFSIVDNLCFTP----TQPTQPTKSTQPTQSTQTTQSTQSTFTTTKAVYRKMGNTLILLRIVNGSGVYPPDYLANTTNPSPYGVKDYKRNFSENFRSILLDDWKRYQPSNLNVTIRDVSDVEVGWLYFSVFPKDNILSWFASTNLIDSFPWNINVLKNSTYNYFSINGDKTYNRSFLVNSQYGGCPNDVGVLMMFDGHNVCNYRLTWLTSATEYPQIIYAELNGSPSNYNNYVRVAKSLEIIGTYPSNIGIF 283
[Close]
BLAST of SMED30008236 vs. Planmine SMEST
Match: SMESG000034377.1 (SMESG000034377.1)

HSP 1 Score: 280.796 bits (717), Expect = 1.181e-94
Identity = 137/271 (50.55%), Postives = 186/271 (68.63%), Query Frame = 1 
Query:   22 GDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSPT--------------------KIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            GD ++   C L+ F + + YF++V+  C+ P     QPTQ + +                    K +Y+++ NT+ +LRIVNGSGVYPPD+L+  +NPS Y VKD + N+SENFRS++LD+W   +PS +NVTIR V++  VGWL F V  +DNI+SWF   NL+DSFPW+INVLKNS Y +FS+ GD+   RSF ++ +Y GC  DVGVLM+FDG + C+Y L W+TSA  YPQI YA LNGSP+ + NY+R AKSLEIIG+YPS+I I+
Sbjct:   17 GDSVEFADCILQTFQKKLTYFSIVDNLCFTP----TQPTQPTKSTQPTQSTQTTQSTQSTFTTTKAVYRKMGNTLILLRIVNGSGVYPPDYLANTTNPSPYGVKDYKRNFSENFRSILLDDWKRYQPSNLNVTIRDVSDVEVGWLYFSVFPKDNILSWFASTNLIDSFPWNINVLKNSTYNYFSINGDKTYNRSFLVNSQYGGCPNDVGVLMVFDGHNVCNYQLMWLTSATEYPQIIYAELNGSPSNYNNYVRVAKSLEIIGTYPSNIGIF 283
[Close]
BLAST of SMED30008236 vs. Planmine SMEST
Match: SMESG000034379.1 (SMESG000034379.1)

HSP 1 Score: 273.863 bits (699), Expect = 8.436e-93
Identity = 137/229 (59.83%), Postives = 163/229 (71.18%), Query Frame = 1 
Query:   88 MVNKSCYIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            MVN  CY P A SAQ T  S  + IY+R+ N +T+LRIVNGSGVYPP +LS +  P  Y+VK+ + NYS NFRS++LDEWS LRPS MNVTI+ V++ IVGWL F V+A DNI SWF LGNLL+SFPWD N+LK   Y FFS+ GDR   RSFFI   Y GC  DVGVLM+FD  DAC Y L WI     YPQI YAV NG PA++ + +R AKSLEIIG Y  ++ IY
Sbjct:    1 MVNNICYTPTAASAQSTLIS-NENIYRRVSNILTLLRIVNGSGVYPPGYLSNMIYPPQYEVKERKGNYSMNFRSILLDEWSTLRPSYMNVTIKDVDDVIVGWLYFVVNASDNISSWFILGNLLNSFPWDTNMLKTKTYNFFSIVGDRTLFRSFFIHSYYGGCDADVGVLMIFDAPDACEYGLAWIVQLNKYPQIIYAVKNGMPALYNDNVRQAKSLEIIGVYSQNVEIY 228
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BLAST of SMED30008236 vs. Planmine SMEST
Match: SMESG000034462.1 (SMESG000034462.1)

HSP 1 Score: 266.929 bits (681), Expect = 4.591e-90
Identity = 134/229 (58.52%), Postives = 161/229 (70.31%), Query Frame = 1 
Query:   88 MVNKSCYIPKAISAQPTQSSPTKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSENFRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGNLLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMMFDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGSYPSDILIY 774
            MVN  CY P A SAQ T  S  + IY+R+ N +T+LRIVNGSGVYPP +LS +  P  Y+VK+ + NYS NFRS++LDEWS LRPS MNVTI+ V++ IVGWL F V++ DNI SWF L NLL+SFPWD N+LK   Y FFS+ GDR   RSFFI   Y GC  DVGVLM+FD  D C Y L WI     YPQI YAV NG PA++ + +R AKSLEIIG Y  ++ IY
Sbjct:    1 MVNNICYTPTAASAQSTLIS-NENIYRRVSNILTLLRIVNGSGVYPPGYLSNMIYPPQYEVKERKGNYSMNFRSILLDEWSTLRPSYMNVTIKDVDDVIVGWLYFVVNSSDNISSWFILDNLLNSFPWDTNMLKTKTYNFFSIVGDRTLFRSFFIHSYYGGCHIDVGVLMIFDAPDVCEYGLAWIVQLNKYPQIIYAVKNGMPALYNDNVRQAKSLEIIGVYSQNVEIY 228
The following BLAST results are available for this feature:
BLAST of SMED30008236 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GLP29.093e-935.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GIN41.425e-833.91Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GLC51.525e-835.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A0L8H6T81.631e-826.90Uncharacterized protein OS=Octopus bimaculoides OX... [more]
A0A1I8G6602.844e-830.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30008236 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30008236 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076267.10.000e+098.07SMESG000076267.1[more]
SMESG000076443.12.132e-9550.92SMESG000076443.1[more]
SMESG000034377.11.181e-9450.55SMESG000034377.1[more]
SMESG000034379.18.436e-9359.83SMESG000034379.1[more]
SMESG000034462.14.591e-9058.52SMESG000034462.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008236 ID=SMED30008236|Name=SMED30008236|organism=Schmidtea mediterranea sexual|type=transcript|length=827bp
GTCTGGTTTTCCGGGAAAAAGGGAGACCCACTTGATGAAATGAGATGCGG
ATTAAAAGCCTTTGACAGAAATATAATATACTTTGCAATGGTCAATAAAT
CTTGCTACATTCCAAAGGCAATATCCGCACAACCAACGCAATCATCGCCA
ACCAAAATCATTTATCAGAGAATAGAAAATACAATAACAGTTTTGAGAAT
TGTCAATGGTAGCGGAGTTTACCCCCCTGATTTTCTATCAAAATTGTCAA
ATCCGTCTACATATAAAGTGAAAGACATACAGAATAACTATTCAGAGAAC
TTTAGAAGCGTCATACTTGATGAATGGAGTGCCTTGCGACCAAGCGGTAT
GAATGTCACGATTAGGGCTGTTAATAATGCTATTGTCGGATGGTTAAATT
TTAGAGTGTCAGCTCAAGACAACATAATATCATGGTTTACGCTTGGCAAT
TTACTGGATTCTTTTCCCTGGGACATAAATGTCTTGAAAAACAGTCTTTA
TACATTCTTTAGTGTTACTGGAGACCGTAAAGGGCTTCGATCATTTTTTA
TTAGTATACGCTATAACGGATGTACCGGTGATGTAGGCGTTTTAATGATG
TTTGACGGGCGTGATGCTTGCAGCTATAATTTGAAATGGATTACCTCAGC
TATTAATTATCCGCAAATATTTTATGCTGTACTGAATGGGAGTCCGGCAA
TATTTTATAATTATATCCGATCCGCGAAGTCATTGGAAATTATTGGGTCT
TATCCGTCTGACATCCTCATTTACGGATGATTTGAACGTTTTTATAACAT
TTTATAACATTATTTATTATATTAGAC
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protein sequence of SMED30008236-orf-1

>SMED30008236-orf-1 ID=SMED30008236-orf-1|Name=SMED30008236-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=260bp
VWFSGKKGDPLDEMRCGLKAFDRNIIYFAMVNKSCYIPKAISAQPTQSSP
TKIIYQRIENTITVLRIVNGSGVYPPDFLSKLSNPSTYKVKDIQNNYSEN
FRSVILDEWSALRPSGMNVTIRAVNNAIVGWLNFRVSAQDNIISWFTLGN
LLDSFPWDINVLKNSLYTFFSVTGDRKGLRSFFISIRYNGCTGDVGVLMM
FDGRDACSYNLKWITSAINYPQIFYAVLNGSPAIFYNYIRSAKSLEIIGS
YPSDILIYG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000099neuron