Calponin-homology (CH) domain-containing protein

Overview
NameCalponin-homology (CH) domain-containing protein
Smed IDSMED30008231
Length (bp)828
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calponin-homology (CH) domain-containing protein (SMED30008231) t-SNE clustered cells

Violin plots show distribution of expression levels for Calponin-homology (CH) domain-containing protein (SMED30008231) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calponin-homology (CH) domain-containing protein (SMED30008231) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calponin-homology (CH) domain-containing protein (SMED30008231) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008231

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30008231SMESG000036289.1 SmedASXL_008069SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008231SMESG000036289.1 Contig49397newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008231SMESG000036289.1 Contig49397uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30008231SMESG000036289.1 dd_Smed_v6_5824_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30008231SMESG000036289.1 dd_Smed_v4_5824_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Human
Match: SPATA4 (spermatogenesis associated 4 [Source:HGNC Symbol;Acc:HGNC:17333])

HSP 1 Score: 148.673 bits (374), Expect = 2.027e-42
Identity = 90/244 (36.89%), Postives = 140/244 (57.38%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLI-RDVSVNQ 773
            K   L R V++WLQ LDLS   +N   D SNG L+ EIF  YY  EL+L+SF NG S+++KL NW  ++ F+ + K  +P+ LIHGTI+ K G    L+ ++Y +LT++      +SI + D   F D  YQ  LPL +RST + +IK+N+ + E+ + P++   + KA+ LI  H ++R+L R   PE F V   +GE  + H PA+ +  +    +   + +  L  I + G   R++ V Q
Sbjct:   47 KSSRLSRSVLRWLQGLDLSFFPRNINRDFSNGFLIAEIFCIYYPWELELSSFENGTSLKVKLDNWAQLEKFLARKKFKLPKELIHGTIHCKAGVPEILIEEVYTLLTHREI----KSIQD-DFVNFTDYSYQMRLPLVSRSTVSKSIKDNIRLSELLSNPNMLTNELKAEFLILLHMLQRKLGRKLNPEWFDVKPTVGEVTLNHLPAQASGRRYNLKVKRGRVVPVLPNIGSGGSSHREIHVKQ 285          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Human
Match: SPATA4 (spermatogenesis associated 4 [Source:HGNC Symbol;Acc:HGNC:17333])

HSP 1 Score: 51.9878 bits (123), Expect = 6.101e-8
Identity = 34/110 (30.91%), Postives = 60/110 (54.55%), Query Frame = 3
Query:  447 LPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLI-RDVSVNQ 773
            LPL +RST + +IK+N+ + E+ + P++   + KA+ LI  H ++R+L R   PE F V   +GE  + H PA+ +  +    +   + +  L  I + G   R++ V Q
Sbjct:    3 LPLVSRSTVSKSIKDNIRLSELLSNPNMLTNELKAEFLILLHMLQRKLGRKLNPEWFDVKPTVGEVTLNHLPAQASGRRYNLKVKRGRVVPVLPNIGSGGSSHREIHVKQ 112          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Human
Match: SPEF1 (sperm flagellar 1 [Source:HGNC Symbol;Acc:HGNC:15874])

HSP 1 Score: 48.521 bits (114), Expect = 3.878e-6
Identity = 24/89 (26.97%), Postives = 49/89 (55.06%), Query Frame = 3
Query:   78 WLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLL 341
            W+ ++ LS+  +N   D S+GVLV E+   Y+ K +++ ++    S++ KL NW +L +  +K+   S+P++++        G    +L
Sbjct:   16 WVDNIPLSRPKRNLSRDFSDGVLVAEVIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLKRLNFSVPDDVMRKIAQCAPGVVELVL 104          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Zebrafish
Match: spata4 (spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-GENE-050417-153])

HSP 1 Score: 157.532 bits (397), Expect = 4.852e-47
Identity = 85/203 (41.87%), Postives = 129/203 (63.55%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIR 653
            K   LPREV+KWLQSLDLS   +N + D SNG LV EIF+ Y+ ++  + SF+NG S+  K  NW  I+ F  K  +S+ + +I GTI+ K GAA  L+++IY ILTN+      ++I  ++   F D  YQ  LP+ AR+TA+V+IK+NL++ E+ AEP++   + K   +I  H  +R+ +R+  P+RF V   +GE+ +R
Sbjct:    8 KKAGLPREVLKWLQSLDLSFFPKNVRRDFSNGYLVAEIFSWYFPRDFQMHSFDNGTSLAAKQSNWSQIEKFFVKQNISLVKEMIDGTIHCKPGAAELLVQEIYTILTNRS----IQAIQRVEQG-FTDKPYQDQLPMVARATASVSIKSNLSLTEVKAEPNIITNQRKVLAIIHRHLEQRKEERVQDPKRFNVKPTLGEQAVR 205          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Zebrafish
Match: spata4 (spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-GENE-050417-153])

HSP 1 Score: 157.532 bits (397), Expect = 4.852e-47
Identity = 85/203 (41.87%), Postives = 129/203 (63.55%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIR 653
            K   LPREV+KWLQSLDLS   +N + D SNG LV EIF+ Y+ ++  + SF+NG S+  K  NW  I+ F  K  +S+ + +I GTI+ K GAA  L+++IY ILTN+      ++I  ++   F D  YQ  LP+ AR+TA+V+IK+NL++ E+ AEP++   + K   +I  H  +R+ +R+  P+RF V   +GE+ +R
Sbjct:    8 KKAGLPREVLKWLQSLDLSFFPKNVRRDFSNGYLVAEIFSWYFPRDFQMHSFDNGTSLAAKQSNWSQIEKFFVKQNISLVKEMIDGTIHCKPGAAELLVQEIYTILTNRS----IQAIQRVEQG-FTDKPYQDQLPMVARATASVSIKSNLSLTEVKAEPNIITNQRKVLAIIHRHLEQRKEERVQDPKRFNVKPTLGEQAVR 205          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Zebrafish
Match: spata4 (spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-GENE-050417-153])

HSP 1 Score: 157.532 bits (397), Expect = 4.852e-47
Identity = 85/203 (41.87%), Postives = 129/203 (63.55%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIR 653
            K   LPREV+KWLQSLDLS   +N + D SNG LV EIF+ Y+ ++  + SF+NG S+  K  NW  I+ F  K  +S+ + +I GTI+ K GAA  L+++IY ILTN+      ++I  ++   F D  YQ  LP+ AR+TA+V+IK+NL++ E+ AEP++   + K   +I  H  +R+ +R+  P+RF V   +GE+ +R
Sbjct:    8 KKAGLPREVLKWLQSLDLSFFPKNVRRDFSNGYLVAEIFSWYFPRDFQMHSFDNGTSLAAKQSNWSQIEKFFVKQNISLVKEMIDGTIHCKPGAAELLVQEIYTILTNRS----IQAIQRVEQG-FTDKPYQDQLPMVARATASVSIKSNLSLTEVKAEPNIITNQRKVLAIIHRHLEQRKEERVQDPKRFNVKPTLGEQAVR 205          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Xenopus
Match: tbc1d19 (TBC1 domain family member 19 [Source:Xenbase;Acc:XB-GENE-5905231])

HSP 1 Score: 160.229 bits (404), Expect = 5.468e-47
Identity = 85/204 (41.67%), Postives = 130/204 (63.73%), Query Frame = 3
Query:   54 NLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPA 665
             +PREV+KWLQSLDLS   +N + D +NG L+ EIF  Y+ +++ L S+ NG S+  +L NW  ++ F  K  L +P+ LI GTI+ K  AA  +++++Y +LTN+   ++++  +E+D   F D  YQ  LP+ ARSTA  T+K+N+T+ EI AEP + + K K Q L+  H  +R+ +R+  P+RF V   +GE  IR PP 
Sbjct:   37 GVPREVLKWLQSLDLSFSPKNFRRDFANGYLIAEIFYWYFPEDIQLHSYENGTSLANRLSNWSQLEKFFMKRNLKVPKALIDGTIHCKPEAAELIIQEMYVMLTNRRIKMIQD--NEVD---FTDCHYQNKLPMVARSTATKTVKSNVTLTEILAEPDIIINKQKVQALMGLHLQQRQQERIEDPKRFNVKPSLGELAIRVPPT 235          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Xenopus
Match: SPEF2 (sperm flagellar 2 [Source:NCBI gene;Acc:100491905])

HSP 1 Score: 48.521 bits (114), Expect = 7.381e-6
Identity = 31/105 (29.52%), Postives = 58/105 (55.24%), Query Frame = 3
Query:   69 VVKWL-QSLDLSKKIQNPKW---DISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNK 371
            + +WL + L LSK+++ PK+   + S+G L+GE+ NK+ ++E D   F+  +    KL N+  I+  ++   +   +N+ H  +  + G A  LL Q++  L  K
Sbjct:    5 ICQWLNEDLKLSKRVE-PKYLAKEFSSGYLIGEVLNKFQLQE-DFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKK 107          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Xenopus
Match: SPEF2 (sperm flagellar 2 [Source:NCBI gene;Acc:100491905])

HSP 1 Score: 48.1358 bits (113), Expect = 9.886e-6
Identity = 31/105 (29.52%), Postives = 57/105 (54.29%), Query Frame = 3
Query:   69 VVKWLQS-LDLSKKIQNPKW---DISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNK 371
            + +WL   L LSK+++ PK+   + S+G L+GE+ NK+ ++E D   F+  +    KL N+  I+  ++   +   +N+ H  +  + G A  LL Q++  L  K
Sbjct:    5 ICQWLNGDLKLSKRVE-PKYLAKEFSSGYLIGEVLNKFQLQE-DFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKK 107          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Mouse
Match: Spata4 (spermatogenesis associated 4 [Source:MGI Symbol;Acc:MGI:1916531])

HSP 1 Score: 131.339 bits (329), Expect = 4.043e-36
Identity = 90/235 (38.30%), Postives = 133/235 (56.60%), Query Frame = 3
Query:   57 LPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKN--KAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIR 755
            L R V++WLQ LDLS   +N   D SNG LV EIF  YY  +L L+SF NG S+++KL NW  I+ F+ K K  +P+ LIHGTI+ K G    L+++IY +LT++    +++     DL+ F D +YQ  LPL  R+T + +IKNN+ + E+ + P  N++ N  K + LI    ++R+L R   P  F V   +GE  I   PA     + +   + EK    L + +N G  R
Sbjct:   50 LSRSVLRWLQGLDLSFFPRNVTRDFSNGYLVAEIFCIYYPWDLRLSSFENGTSLKVKLDNWAQIEKFLAKKKFKLPKELIHGTIHCKAGVPEILIQEIYTLLTHQEIRSIQD-----DLANFTDYIYQMRLPLVPRNTVSKSIKNNIRLSELLSNP--NVLSNELKIEFLILLQMLQRKLSRKLNPGWFDVKPTVGEITIDRLPAHSYKRRYKSRGSKEKAAQPLSKSDNDGNAR 277          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Mouse
Match: Spef1 (sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:3513546])

HSP 1 Score: 47.3654 bits (111), Expect = 6.125e-6
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 3
Query:   78 WLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLL 341
            W+ ++ LS+  +N   D S+GVLV E+   Y+ K +++ ++    S++ KL NW +L +  + K   S+P++++        G    +L
Sbjct:   16 WVDNIPLSRPKRNLSRDFSDGVLVAELIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVELVL 104          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Mouse
Match: Spef1 (sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:3513546])

HSP 1 Score: 47.3654 bits (111), Expect = 6.125e-6
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 3
Query:   78 WLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLL 341
            W+ ++ LS+  +N   D S+GVLV E+   Y+ K +++ ++    S++ KL NW +L +  + K   S+P++++        G    +L
Sbjct:   16 WVDNIPLSRPKRNLSRDFSDGVLVAELIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVELVL 104          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Mouse
Match: Spef1 (sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:3513546])

HSP 1 Score: 46.2098 bits (108), Expect = 8.875e-6
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 3
Query:   78 WLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLL 341
            W+ ++ LS+  +N   D S+GVLV E+   Y+ K +++ ++    S++ KL NW +L +  + K   S+P++++        G    +L
Sbjct:   10 WVDNIPLSRPKRNLSRDFSDGVLVAELIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVELVL 98          
BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6DMN8|SPAT4_PANTR (Spermatogenesis-associated protein 4 OS=Pan troglodytes OX=9598 GN=SPATA4 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 7.867e-42
Identity = 90/244 (36.89%), Postives = 140/244 (57.38%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLI-RDVSVNQ 773
            K   L R V++WLQ LDLS   +N   D SNG L+ EIF  YY  EL+L+SF NG S+++KL NW  ++ F+ + K  +P+ LIHGTI+ K G    L+ ++Y +LT++      +SI + D   F D  YQ  LPL +RST + +IK+N+ + E+ + P++   + KA+ LI  H ++R+L R   PE F V   +GE  + H PA+ +  +    +   + +  L  I + G   R++ V Q
Sbjct:   47 KSSRLSRSVLRWLQGLDLSFFPRNINRDFSNGFLIAEIFCIYYPWELELSSFENGTSLKVKLGNWAQLEKFLARKKFKLPKELIHGTIHCKAGVPEILIEEVYTLLTHREI----KSIQD-DFVNFTDYSYQMRLPLVSRSTVSKSIKDNIRLSELLSNPNMLTNELKAEFLILLHMLQRKLGRKLNPEWFDVKPTVGEVTLNHLPAQASGRRYNLKVKRGRVVPVLPNIGSGGSSHREIHVKQ 285          
BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8NEY3|SPAT4_HUMAN (Spermatogenesis-associated protein 4 OS=Homo sapiens OX=9606 GN=SPATA4 PE=1 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 9.734e-42
Identity = 90/244 (36.89%), Postives = 140/244 (57.38%), Query Frame = 3
Query:   45 KMFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLI-RDVSVNQ 773
            K   L R V++WLQ LDLS   +N   D SNG L+ EIF  YY  EL+L+SF NG S+++KL NW  ++ F+ + K  +P+ LIHGTI+ K G    L+ ++Y +LT++      +SI + D   F D  YQ  LPL +RST + +IK+N+ + E+ + P++   + KA+ LI  H ++R+L R   PE F V   +GE  + H PA+ +  +    +   + +  L  I + G   R++ V Q
Sbjct:   47 KSSRLSRSVLRWLQGLDLSFFPRNINRDFSNGFLIAEIFCIYYPWELELSSFENGTSLKVKLDNWAQLEKFLARKKFKLPKELIHGTIHCKAGVPEILIEEVYTLLTHREI----KSIQD-DFVNFTDYSYQMRLPLVSRSTVSKSIKDNIRLSELLSNPNMLTNELKAEFLILLHMLQRKLGRKLNPEWFDVKPTVGEVTLNHLPAQASGRRYNLKVKRGRVVPVLPNIGSGGSSHREIHVKQ 285          
BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8K3V1|SPAT4_MOUSE (Spermatogenesis-associated protein 4 OS=Mus musculus OX=10090 GN=Spata4 PE=1 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 2.828e-35
Identity = 90/235 (38.30%), Postives = 133/235 (56.60%), Query Frame = 3
Query:   57 LPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKN--KAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIR 755
            L R V++WLQ LDLS   +N   D SNG LV EIF  YY  +L L+SF NG S+++KL NW  I+ F+ K K  +P+ LIHGTI+ K G    L+++IY +LT++    +++     DL+ F D +YQ  LPL  R+T + +IKNN+ + E+ + P  N++ N  K + LI    ++R+L R   P  F V   +GE  I   PA     + +   + EK    L + +N G  R
Sbjct:   50 LSRSVLRWLQGLDLSFFPRNVTRDFSNGYLVAEIFCIYYPWDLRLSSFENGTSLKVKLDNWAQIEKFLAKKKFKLPKELIHGTIHCKAGVPEILIQEIYTLLTHQEIRSIQD-----DLANFTDYIYQMRLPLVPRNTVSKSIKNNIRLSELLSNP--NVLSNELKIEFLILLQMLQRKLSRKLNPGWFDVKPTVGEITIDRLPAHSYKRRYKSRGSKEKAAQPLSKSDNDGNAR 277          
BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Match: sp|Q0IH24|SPEF1_XENLA (Sperm flagellar protein 1 OS=Xenopus laevis OX=8355 GN=spef1 PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.993e-6
Identity = 27/107 (25.23%), Postives = 55/107 (51.40%), Query Frame = 3
Query:   63 REVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLI-QNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQL 380
            +E+  W+ ++ LS+  +N   D S+GVL  E+   Y+ K +++ ++    S   KL NW ++ +  + K   S+P+++I   +    G    +L  + Q +  K +L
Sbjct:   11 QELYTWVDTIPLSRPKRNIARDFSDGVLTAELVKFYFPKLVEMHNYVPANSTTQKLSNWTILNRKVLSKLSFSVPDDVIRKIVQCSPGVVELVLNTLRQKIEEKQRL 117          
BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Match: sp|Q58DA1|SPEF1_BOVIN (Sperm flagellar protein 1 OS=Bos taurus OX=9913 GN=SPEF1 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 7.821e-6
Identity = 25/89 (28.09%), Postives = 48/89 (53.93%), Query Frame = 3
Query:   78 WLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLL 341
            W+ ++ LS+  +N   D S+GVLV E+   Y+ K +++ ++    S++ KL NW +L +  + K   S+PE+++        G    +L
Sbjct:   16 WVDNIPLSRPKRNLSRDFSDGVLVAEVIKFYFPKMVEMHNYVPANSLQQKLSNWSHLNRKVLNKLNFSVPEDVMRKIAQCAPGVVELVL 104          
BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Match: K1QF98 (Spermatogenesis-associated protein 4 OS=Crassostrea gigas OX=29159 GN=CGI_10006510 PE=4 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 1.341e-60
Identity = 102/203 (50.25%), Postives = 141/203 (69.46%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLR--ESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCI 650
            M  LPREV+KW+QSLDL+ +I+  KWD++NG L+ EIF+ Y+ +++ + S+NNG S++ KLKNW L++ FIK++KL IPE LI GTI+ KEGAA  L  +IY++LTN+  +  R  +  SE +   F DS YQ  LP+HARSTA+  +KNNL I E  A+P+L L   KAQ +I DH   R L+R   PERF V   +GE+C+
Sbjct:    1 MSGLPREVLKWIQSLDLTWQIKQAKWDLTNGYLIAEIFSWYFPQDIQMHSYNNGTSLDSKLKNWSLLKLFIKRHKLEIPEELIEGTIHCKEGAAPALCERIYELLTNRKWVSARVKKQPSEFEAD-FTDSAYQLQLPMHARSTASKAVKNNLRITETMADPNLILNAQKAQKIINDHIQHRNLERFENPERFDVKPTLGEKCL 202          
BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Match: A0A1S3HMP1 (spermatogenesis-associated protein 4 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106156196 PE=4 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 6.168e-59
Identity = 99/200 (49.50%), Postives = 137/200 (68.50%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERC 647
            M  L REVV+WLQSLDL+ +++ PKWD+SNG L+ EIF+ YY +E+ + SFNNG S+++KL NW++++NF K++K +IPE  + GTI+ KEGAA  L+  IY+ILTN+ Q+    S  EID   F D  YQ+ LP+HARSTA+  +KNNL I E+ A+ S  L + KAQ +I  H   R  +RL  P RF +   +GER 
Sbjct:    1 MAGLAREVVRWLQSLDLTWQVKTPKWDLSNGYLIAEIFSWYYPQEITMHSFNNGTSLDMKLLNWHVLKNFFKRHKFAIPEEYVEGTIHCKEGAAKLLIESIYEILTNR-QVRRLPSEYEID---FTDRQYQEKLPMHARSTASQAVKNNLRITELMADNSFILGQQKAQEIIGSHVEHRRQERLEDPRRFGIKPTLGERS 196          
BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Match: A0A210QYL8 (Spermatogenesis-associated protein 4 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT12200 PE=4 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 9.067e-59
Identity = 100/207 (48.31%), Postives = 140/207 (67.63%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAE 668
            M  LPREV+KW+QSLDL+ +I+ PKWD++NG LV E+F+ Y+ +E+ + S+NNG S++ KLKNW L++ FIK++ L I E L+ GTI+ KEGA+  L  +IY+ILTN+ ++  +    EID   F D  YQ  LP+HARSTA+  +KNNL I E  A+ +L L   K+Q +I DH   R  +R   P RF V   +GER +R PPA+
Sbjct:    1 MSGLPREVLKWIQSLDLTWQIKTPKWDLTNGYLVAEVFSWYFPQEIQMHSYNNGTSLDSKLKNWSLLRLFIKRHNLDIAEELLEGTIHCKEGASTLLTERIYEILTNR-KVRKQTKDDEID---FTDRAYQNRLPMHARSTASKAVKNNLRITETMADQNLILSAQKSQKIINDHIEHRRQERYENPVRFNVKPTLGERSLRRPPAQ 203          
BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Match: A0A2T7PPC0 (CH_2 domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_06531 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.234e-57
Identity = 95/207 (45.89%), Postives = 135/207 (65.22%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAE 668
            M  LPRE++KWLQSLDL+ K++ PKWD++NG L+ EIF+ Y+ +E+ + SF N  S+ +K KNW L++NFIK  +L IPE  I GTI+ KEGAA  LL  +Y++LT++    ++  + E     + D  YQ  LP+HARSTA+  +KNNL   EI A+P L +   K   +I DH   R L+R+  P RF +   +GERC+R P +E
Sbjct:    1 MSGLPREILKWLQSLDLTWKVKTPKWDLTNGYLLAEIFSWYFPQEIQMHSFYNCNSLPIKQKNWSLLKNFIKSKELKIPEQYIEGTIHCKEGAAALLLESMYELLTHRKVTKMKSEMPED----YTDYAYQVKLPMHARSTASKAVKNNLRNTEILADPCLIVNAQKCHKIISDHVDHRHLERILDPVRFGIKPSVGERCLRKPLSE 203          
BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Match: A0A3M7QI75 (Spermatogenesis-associated 4-like isoform X2 OS=Brachionus plicatilis OX=10195 GN=BpHYR1_009950 PE=4 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 9.016e-57
Identity = 102/211 (48.34%), Postives = 136/211 (64.45%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTN----KGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAE 668
            M  L R VVKW+QSLDL+ +I+NPKWD++NG LV EIF+ YY  E+ + +FNNG+S++ KL NW LI+ FI K ++ I   LI GTI+ KEGAA+ LL  +YQ LTN    K QL       EIDL+   D  YQ  LP HARSTAA +IK N+   E+  +P+L L   K + LI +H   R+ DR + P+RF V + I E+C++ P  E
Sbjct:    1 MSGLSRSVVKWIQSLDLTFQIKNPKWDLTNGFLVAEIFSWYYPDEIKMFTFNNGKSLDSKLSNWSLIKKFITKNEMDISNELIDGTIHCKEGAAVLLLENLYQFLTNRPIQKAQLEY-----EIDLT---DKGYQMRLPYHARSTAAKSIKTNIKNTELLTDPNLKLSAQKVEILISNHVENRKSDRENEPQRFDVKKTIAEKCVQKPVPE 203          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Cavefish
Match: spata4 (spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-GENE-050417-153])

HSP 1 Score: 134.035 bits (336), Expect = 9.342e-38
Identity = 68/186 (36.56%), Postives = 114/186 (61.29%), Query Frame = 3
Query:  108 IQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPA 665
            ++ P  D SNG LV EIF+++Y ++  + S+ NG S+  K  NW  I+  + K +LS+ +  + GTI+ K GAA  L+++IY ++TN+    ++E   +     F D  YQ+ LP+ AR+TA+  IKNNL + E  A+P++   + K Q++I  H  +R+ +R+ +P+RF V   +GE+ +R PP 
Sbjct:   15 LKTPSQDFSNGFLVAEIFSRFYPEDFPMHSYGNGTSLPTKQSNWSQIEKGLMKLQLSLVKEAVDGTIHCKPGAAEALVQEIYTLMTNRRISSVQEGALD-----FTDLSYQEQLPIVARATASKAIKNNLRLSEEIADPNITATQQKIQNIIHRHVERRKDERIRHPKRFGVKPTLGEQAVRLPPT 195          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Cavefish
Match: zgc:66426 (sperm flagellar 1 [Source:NCBI gene;Acc:103038874])

HSP 1 Score: 49.6766 bits (117), Expect = 7.390e-7
Identity = 24/79 (30.38%), Postives = 43/79 (54.43%), Query Frame = 3
Query:   63 REVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNW-YLIQNFIKKYKLSIPENLI 296
            +E+  W+  ++LS+  +N   D S+GV++ E+   +Y K +DL ++    S + KL NW  L +    K    IPE+ +
Sbjct:   14 QELYAWIDQINLSRPKRNIARDFSDGVMIAEVVKHFYPKLVDLHNYTPAHSTQQKLSNWNTLNRKVFSKLNFHIPEDTV 92          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Sea Lamprey
Match: spata4 (spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-GENE-050417-153])

HSP 1 Score: 159.844 bits (403), Expect = 1.946e-48
Identity = 81/203 (39.90%), Postives = 124/203 (61.08%), Query Frame = 3
Query:   54 NLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPP 662
             L REV +WLQSLDL+ + +N + D+SNG+++ EIF+ YY  +L + SF  G+S+  KL NW L+  F  K  L+  + L  GT++ K GAA  LL+++Y  LTN+     R   S  +   F D+ YQ+ LP+HARSTA+  +K+N+ + ++ AEPS    + +A+ ++ DH  +R  +R   P RF V   +GE  +R PP
Sbjct:    5 GLSREVFRWLQSLDLTVQFRNARRDLSNGLVLAEIFSWYYPGDLLMHSFQPGESLASKLANWSLLHRFFAKRGLAFSKELTDGTLHRKPGAAEQLLQRVYSTLTNR-----RAQESNSEAPRFTDARYQRALPMHARSTASQALKSNVRLTQLLAEPSEVATRQRAEVVLRDHLAQRRREREQDPCRFDVRPTLGELAVRLPP 202          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Nematostella
Match: EDO40759 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S652])

HSP 1 Score: 179.874 bits (455), Expect = 9.083e-56
Identity = 98/246 (39.84%), Postives = 145/246 (58.94%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFG----LIRDVSVNQ 773
            M  LPRE++KW+QSLDLS  ++N K D SNG LV EIF+ YY +E+ + S++NG S+  K+ NW  +Q F ++    IP+ +I GTI+ K GAA  L+ +IY +LTN+    +R +  E+D   F D+ YQ  LP HARSTA++ IKNN+   E++ +P   L + KAQ +I  H   R  +R   P RF +   +GE C R  P   + + R    N +++  + QE++  G       +V VNQ
Sbjct:    1 MSGLPREILKWIQSLDLSHPVKNTKRDFSNGCLVAEIFSWYYPQEIQMHSYDNGASLATKMGNWSQLQTFFQRQGFGIPKEVIDGTIHCKPGAAALLVEKIYTLLTNRVVKKVR-TDDELD---FTDTGYQAQLPAHARSTASMAIKNNIANTELSTDPDRILCQQKAQSIINSHVEHRRKERSDDPHRFGIKPTLGELCPRSAPRPSHHLSRNTEENFQQKQTSEQELSFSGEPPAHYTEVQVNQ 242          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Nematostella
Match: EDO30183 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1I3])

HSP 1 Score: 170.244 bits (430), Expect = 7.087e-53
Identity = 87/202 (43.07%), Postives = 124/202 (61.39%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIR 653
            M  LPRE++KW+QSLDLS  ++N K D SNG LV EIF+ YY +E+ + S++NG S+  K+ NW  +Q F ++    I + +I GTI+ K GAA  L+ +IY +LTN+    +R    E+D   F D+ YQ  LP HARSTA++ IKNN+   E++ +P   L + KAQ +I  H   R  +R   P RF +   +GE C R
Sbjct:    1 MSGLPREILKWIQSLDLSHPVKNTKRDFSNGCLVAEIFSWYYPQEIQMHSYDNGASLATKMGNWSQLQTFFQRQGFGISKEVIDGTIHCKPGAAALLVEKIYTLLTNRVVKKVRTD-DELD---FTDTGYQAQLPAHARSTASMAIKNNIANTELSTDPDRILCQQKAQSIINSHVEHRRKERSDDPHRFGIKPTLGELCPR 198          
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Nematostella
Match: EDO34469 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPA4])

HSP 1 Score: 48.521 bits (114), Expect = 6.715e-7
Identity = 28/99 (28.28%), Postives = 51/99 (51.52%), Query Frame = 3
Query:   63 REVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQN--FIKKYKLSIPENLIHGTIYMKEGA---AIFLLR 344
            +E+  W+  + LS+  ++   D S+GVLV EI   +  K +D+ +++   S+  KL NW  +      K    ++ +N I+  +  K GA    +F+LR
Sbjct:   19 QELFTWIDEIPLSRPKKSITRDFSDGVLVAEIVKNFLPKLVDIHNYSPANSLTQKLTNWSTLNRKVLTKHLNYTVSDNTINNIVNCKAGAIEHVLFVLR 117          
BLAST of Calponin-homology (CH) domain-containing protein vs. Planmine SMEST
Match: SMESG000036289.1 (SMESG000036289.1)

HSP 1 Score: 508.064 bits (1307), Expect = 0.000e+0
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS 794
            MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS
Sbjct:    1 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS 249          
BLAST of Calponin-homology (CH) domain-containing protein vs. Planmine SMEST
Match: SMESG000036289.1 (SMESG000036289.1)

HSP 1 Score: 491.886 bits (1265), Expect = 1.063e-178
Identity = 244/249 (97.99%), Postives = 244/249 (97.99%), Query Frame = 3
Query:   48 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS 794
            MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKG     ESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS
Sbjct:    1 MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQIYQILTNKG-----ESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNLTIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERCIRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS 244          
BLAST of Calponin-homology (CH) domain-containing protein vs. Planmine SMEST
Match: SMESG000014677.1 (SMESG000014677.1)

HSP 1 Score: 47.7506 bits (112), Expect = 2.438e-6
Identity = 26/89 (29.21%), Postives = 48/89 (53.93%), Query Frame = 3
Query:   63 REVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLASFNNGQSIELKLKNWYLIQ-NFIKKYKLSIPENLIHGTIYMKEGA 326
            +++  W+ ++ LS+  +N   D S+GVL+ EI   Y+ K +++ S+    S+  K KNW+L+     KK    + +++I   I  K G 
Sbjct:   10 QDLYAWVDTIPLSRPKKNISRDFSDGVLMAEIVKHYFPKLIEIHSYPACNSLTQKKKNWHLLNWKAFKKLNFELSDDVITNLIDAKAGT 98          
The following BLAST results are available for this feature:
BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SPATA42.027e-4236.89spermatogenesis associated 4 [Source:HGNC Symbol;A... [more]
SPATA46.101e-830.91spermatogenesis associated 4 [Source:HGNC Symbol;A... [more]
SPEF13.878e-626.97sperm flagellar 1 [Source:HGNC Symbol;Acc:HGNC:158... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
spata44.852e-4741.87spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-... [more]
spata44.852e-4741.87spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-... [more]
spata44.852e-4741.87spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tbc1d195.468e-4741.67TBC1 domain family member 19 [Source:Xenbase;Acc:X... [more]
SPEF27.381e-629.52sperm flagellar 2 [Source:NCBI gene;Acc:100491905][more]
SPEF29.886e-629.52sperm flagellar 2 [Source:NCBI gene;Acc:100491905][more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Spata44.043e-3638.30spermatogenesis associated 4 [Source:MGI Symbol;Ac... [more]
Spef16.125e-626.97sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:35135... [more]
Spef16.125e-626.97sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:35135... [more]
Spef18.875e-626.97sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:35135... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6DMN8|SPAT4_PANTR7.867e-4236.89Spermatogenesis-associated protein 4 OS=Pan troglo... [more]
sp|Q8NEY3|SPAT4_HUMAN9.734e-4236.89Spermatogenesis-associated protein 4 OS=Homo sapie... [more]
sp|Q8K3V1|SPAT4_MOUSE2.828e-3538.30Spermatogenesis-associated protein 4 OS=Mus muscul... [more]
sp|Q0IH24|SPEF1_XENLA1.993e-625.23Sperm flagellar protein 1 OS=Xenopus laevis OX=835... [more]
sp|Q58DA1|SPEF1_BOVIN7.821e-628.09Sperm flagellar protein 1 OS=Bos taurus OX=9913 GN... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1QF981.341e-6050.25Spermatogenesis-associated protein 4 OS=Crassostre... [more]
A0A1S3HMP16.168e-5949.50spermatogenesis-associated protein 4 isoform X1 OS... [more]
A0A210QYL89.067e-5948.31Spermatogenesis-associated protein 4 OS=Mizuhopect... [more]
A0A2T7PPC05.234e-5745.89CH_2 domain-containing protein OS=Pomacea canalicu... [more]
A0A3M7QI759.016e-5748.34Spermatogenesis-associated 4-like isoform X2 OS=Br... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
spata49.342e-3836.56spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-... [more]
zgc:664267.390e-730.38sperm flagellar 1 [Source:NCBI gene;Acc:103038874][more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spata41.946e-4839.90spermatogenesis associated 4 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO407599.083e-5639.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO301837.087e-5343.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO344696.715e-728.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calponin-homology (CH) domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calponin-homology (CH) domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000036289.10.000e+0100.00SMESG000036289.1[more]
SMESG000036289.11.063e-17897.99SMESG000036289.1[more]
SMESG000014677.12.438e-629.21SMESG000014677.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008231 ID=SMED30008231|Name=Calponin-homology (CH) domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=828bp
TGACGAGTTTGTTTTACTTATTGTTACCTAGTTAAAAAAATTGTAAAATG
TTTAATTTACCCAGAGAAGTAGTGAAATGGTTGCAGAGTCTTGACCTTTC
AAAAAAAATTCAAAATCCCAAATGGGATATTTCAAATGGAGTTTTGGTCG
GAGAGATTTTTAACAAATATTATATAAAAGAATTAGATTTAGCTTCATTT
AACAATGGACAATCTATTGAACTGAAATTGAAAAATTGGTATTTAATCCA
AAATTTTATTAAAAAATACAAATTATCCATTCCAGAAAATTTGATTCATG
GAACTATTTACATGAAAGAAGGGGCCGCTATTTTTCTATTAAGACAGATT
TATCAAATACTAACTAATAAAGGTCAACTTTTATTAAGAGAATCTATCTC
AGAAATAGATCTCTCACTGTTCAATGACTCGATGTACCAACAAGGATTAC
CACTCCATGCCAGGTCTACAGCAGCGGTGACAATTAAAAATAATCTAACC
ATCCATGAGATTGCTGCTGAACCGTCTCTGAATTTAATCAAAAATAAAGC
GCAGCATTTAATATTTGACCATCGCATGAAACGTGAATTAGACCGCTTGA
GTTACCCTGAACGATTCCAGGTCCACAGGAAGATAGGAGAGAGATGCATT
CGACACCCACCGGCTGAAGACAATGCAATCCAGAGAGAGAAAAGTATAAA
TAATGAAAAAGAAATTTACAACTTACAGGAAATTAACAATTTCGGACTGA
TTCGGGATGTTTCCGTGAACCAGCCATCGAAAGTCGAAGCAAGTTGACAA
ATGCTGCTTGAGCCGTGAGATCGGAAGA
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protein sequence of SMED30008231-orf-1

>SMED30008231-orf-1 ID=SMED30008231-orf-1|Name=SMED30008231-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
MFNLPREVVKWLQSLDLSKKIQNPKWDISNGVLVGEIFNKYYIKELDLAS
FNNGQSIELKLKNWYLIQNFIKKYKLSIPENLIHGTIYMKEGAAIFLLRQ
IYQILTNKGQLLLRESISEIDLSLFNDSMYQQGLPLHARSTAAVTIKNNL
TIHEIAAEPSLNLIKNKAQHLIFDHRMKRELDRLSYPERFQVHRKIGERC
IRHPPAEDNAIQREKSINNEKEIYNLQEINNFGLIRDVSVNQPSKVEAS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007034vacuolar transport
GO:0030001metal ion transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005575cellular_component
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036872CH_dom_sf
IPR010441CH_2
IPR001715CH-domain
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003674molecular_function
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010441CH-like domain in sperm proteinPFAMPF06294CH_2coord: 8..103
e-value: 7.1E-27
score: 93.4
IPR036872CH domain superfamilyGENE3DG3DSA:1.10.418.10coord: 4..111
e-value: 2.1E-30
score: 106.8
IPR036872CH domain superfamilySUPERFAMILYSSF47576Calponin-homology domain, CH-domaincoord: 6..92
NoneNo IPR availablePANTHERPTHR12509:SF8SPERMATOGENESIS-ASSOCIATED PROTEIN 4coord: 4..166
NoneNo IPR availablePANTHERPTHR12509SPERMATOGENESIS-ASSOCIATED 4-RELATEDcoord: 4..166
IPR001715Calponin homology domainPROSITEPS50021CHcoord: 2..106
score: 10.619