SMED30008229
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008229 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30008229 aligns in the following genomic locations:
Homology
BLAST of SMED30008229 vs. Ensembl Zebrafish
Match: fbxl13 (F-box and leucine-rich repeat protein 13 [Source:ZFIN;Acc:ZDB-GENE-140106-120]) HSP 1 Score: 63.5438 bits (153), Expect = 4.782e-11 Identity = 45/152 (29.61%), Postives = 80/152 (52.63%), Query Frame = -1 Query: 239 YVDKGRLTQIYESLICGLCVMKPKKPQLWILDK-LRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSNDEYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNE-SKLEEIALFHYQVKWISRIFIAWKLWTVHRLQR 688 Y+ K L QIY++L+ GLCV P+ P ++ +K L +++ D++ + W+TFID+ + + I+++IF ++ +L AY+ Y+ ++ CF W Y KK + ++EE ++H Q + I FI W W + QR Sbjct: 14 YIIKHSLPQIYKALLSGLCVSCPEDPLHFMEEKILSILQDQDFE-IVWHTFIDK--DKQISSLAGGIVNYIFGYPEDSLNLSHLLEKAYTCYHTNLTKMCFTGWKSYIWQKKMKAARLLERMEEAEMYHTQ-RRIKLAFIKWTAWVQFQKQR 161
BLAST of SMED30008229 vs. TrEMBL
Match: A0A4Z2DTX9 (F-box/LRR-repeat protein (Fragment) OS=Schistosoma japonicum OX=6182 GN=EWB00_005971 PE=4 SV=1) HSP 1 Score: 109.383 bits (272), Expect = 2.729e-23 Identity = 56/158 (35.44%), Postives = 92/158 (58.23%), Query Frame = -1 Query: 257 PLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSND---------EYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWT 703 P L+KY K L +++E+L+ GL VM P P+ WI +KL+L+ + + L W+TFID M+P HP+V ++ +F ++ +YQPT EM++ AY+A +SI+ +C +AW +Y + K+ R + K+ IA + + IF WK +T Sbjct: 12 PNLIKYTRKHHLYEVFEALLGGLSVMLPTNPRRWIAEKLQLLYDIGFISLNWDTFIDPDMKPTHPYVDHELMHSLFGTSKLEFLLPEELQYQPTPEMIAKAYAAQKQSILKKCLSAWKLYFIVKRARTTHICKINFIAEMTRKCRLKKTIFQTWKEYT 169
BLAST of SMED30008229 vs. TrEMBL
Match: A0A3R7F818 (F-box/LRR-repeat protein 13 OS=Clonorchis sinensis OX=79923 GN=Fbxl13 PE=4 SV=1) HSP 1 Score: 106.301 bits (264), Expect = 3.760e-22 Identity = 57/166 (34.34%), Postives = 94/166 (56.63%), Query Frame = -1 Query: 236 PLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIF--------KSNDEYQPTIEMLSIAYSAYNRSIISRCFNAW-VIYHVSKKKRHGNESKLEEIALFHYQVKWISR-IFIAWKLWTVHRLQRQ 703 PLL KYVD L ++E+LI GL VM P+ P++WI++KL + + ++++W++FID MRP+HPFV + + F +D YQPT EM A +N++++ +CF AW + H+ +R ++ + + F + W R F AW WT + +R+ Sbjct: 8 PLLSKYVDDNYLYNVFEALISGLAVMLPEAPRVWIVEKLGKVYDIGNENVRWDSFIDPRMRPQHPFVAQDLFRGWFWRARIHSVSPSDRYQPTSEMFVKACHTFNQNLLKKCFRAWKQLVHIKAARRIQKQTMILKANKF--RKLWALRYAFDAWSNWTSNISKRR 171
BLAST of SMED30008229 vs. TrEMBL
Match: A0A1S3JMT0 (F-box/LRR-repeat protein 13-like isoform X2 OS=Lingula unguis OX=7574 GN=LOC106174596 PE=4 SV=1) HSP 1 Score: 102.834 bits (255), Expect = 4.210e-21 Identity = 59/154 (38.31%), Postives = 85/154 (55.19%), Query Frame = -1 Query: 248 PLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSND-EYQPTIEMLSIAYSAYNRSIISRCFNAWVIYH-VSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHR 703 P L KY+ RL +YE+L+ GL VM P P+ +I+ LR I+ LQW+ F++E MRP+ + E+ L +IF +D QPT EM + A+S YN + S CF+ W+ +H V K+KR E K E A+ HY + + W W R Sbjct: 10 PELKKYLRINRLPDVYEALLTGLAVMCPDDPKAFIVKCLRHIQKYGLRCLQWDMFVEEEMRPRRRVITESNLDYIFNLDDYSLQPTPEMYAKAFSFYNSRLRSMCFHGWMQFHLVKKQKRMDMERKWCE-AVTHYAHRLMRLALNEWTQWMNFR 162
BLAST of SMED30008229 vs. TrEMBL
Match: A0A1S3JNE8 (F-box/LRR-repeat protein 13-like isoform X1 OS=Lingula unguis OX=7574 GN=LOC106174596 PE=4 SV=1) HSP 1 Score: 102.834 bits (255), Expect = 4.464e-21 Identity = 59/154 (38.31%), Postives = 85/154 (55.19%), Query Frame = -1 Query: 248 PLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSND-EYQPTIEMLSIAYSAYNRSIISRCFNAWVIYH-VSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHR 703 P L KY+ RL +YE+L+ GL VM P P+ +I+ LR I+ LQW+ F++E MRP+ + E+ L +IF +D QPT EM + A+S YN + S CF+ W+ +H V K+KR E K E A+ HY + + W W R Sbjct: 10 PELKKYLRINRLPDVYEALLTGLAVMCPDDPKAFIVKCLRHIQKYGLRCLQWDMFVEEEMRPRRRVITESNLDYIFNLDDYSLQPTPEMYAKAFSFYNSRLRSMCFHGWMQFHLVKKQKRMDMERKWCE-AVTHYAHRLMRLALNEWTQWMNFR 162
BLAST of SMED30008229 vs. TrEMBL
Match: A0A1S3JMS7 (F-box/LRR-repeat protein 13-like isoform X3 OS=Lingula unguis OX=7574 GN=LOC106174596 PE=4 SV=1) HSP 1 Score: 102.834 bits (255), Expect = 5.044e-21 Identity = 59/154 (38.31%), Postives = 85/154 (55.19%), Query Frame = -1 Query: 248 PLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSND-EYQPTIEMLSIAYSAYNRSIISRCFNAWVIYH-VSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHR 703 P L KY+ RL +YE+L+ GL VM P P+ +I+ LR I+ LQW+ F++E MRP+ + E+ L +IF +D QPT EM + A+S YN + S CF+ W+ +H V K+KR E K E A+ HY + + W W R Sbjct: 10 PELKKYLRINRLPDVYEALLTGLAVMCPDDPKAFIVKCLRHIQKYGLRCLQWDMFVEEEMRPRRRVITESNLDYIFNLDDYSLQPTPEMYAKAFSFYNSRLRSMCFHGWMQFHLVKKQKRMDMERKWCE-AVTHYAHRLMRLALNEWTQWMNFR 162
BLAST of SMED30008229 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001587.1 (pep scaffold:Pmarinus_7.0:GL477300:34863:67547:1 gene:ENSPMAG00000001430.1 transcript:ENSPMAT00000001587.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 80.1073 bits (196), Expect = 1.991e-17 Identity = 44/147 (29.93%), Postives = 71/147 (48.30%), Query Frame = -1 Query: 221 IYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSNDEYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHRLQRQKGKVL 661 + +L+ LCV+ P+ P ++L+K+R I+ L W FIDE +RPK V L+FIF+ +D+ PT + AYS R + F W +Y ++ R ++ A HY K + ++W W R +R G + Sbjct: 23 LCHALLTALCVLCPENPLEFLLNKMREIQEFGLHTLDWEMFIDESLRPKLKRVTAGYLTFIFRFDDD-APTPQQFEKAYSFLRRKLQREYFVRWAMYGARRRARREQLTRALHRACDHYMRKKLGAALLSWSQWLHARQERMAGAAI 168
BLAST of SMED30008229 vs. Ensembl Nematostella
Match: EDO48070 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK01]) HSP 1 Score: 73.9442 bits (180), Expect = 4.834e-15 Identity = 47/147 (31.97%), Postives = 78/147 (53.06%), Query Frame = -1 Query: 236 QIYESLICGLCVMKPKKPQLWILDKLRLIRTMD--YDDLQWNTFIDEWMRPKHPFVIETILSFIFKSNDE-YQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVS-KKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHRLQRQ 664 ++E+++ L + P PQ +IL KL L+ + +DL+W++FIDE +PK+ + L F++ DE QPT EM AY+ YN + CF+AW+ +H ++K+ + KL +H Q + + W W R +RQ Sbjct: 22 DVFEAILTALAINCPDDPQEFILSKLTLVLGDEQVLEDLRWDSFIDEMYKPKNRIIKTAALEFLWSYEDENSQPTPEMYQKAYNLYNGRLKVMCFSAWLRFHRHMRRKKMILQRKLNNARTWHRQ-RILRLQLQKWIEWVAERKERQ 167
BLAST of SMED30008229 vs. Ensembl Nematostella
Match: EDO28748 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5L3]) HSP 1 Score: 59.3066 bits (142), Expect = 1.262e-11 Identity = 34/94 (36.17%), Postives = 53/94 (56.38%), Query Frame = -1 Query: 389 IYESLICGLCVMKPKKPQLWILDKLRLIRTMD--YDDLQWNTFIDEWMRPKHPFVIETILSFIFKSNDE-YQPTIEMLSIAYSAYNRSIISRCF 661 I ++++ L + P PQ +IL KL L+ + +DL+W++FIDE +PK+ + L F++ DE QPT EM AY+ YN + CF Sbjct: 12 IPQAILTALAINCPDDPQEFILSKLTLVLGDEQVLEDLRWDSFIDEMYKPKNRIIKTAALEFLWSYEDENSQPTPEMYQKAYNLYNGKLKVMCF 105
BLAST of SMED30008229 vs. Planmine SMEST
Match: SMESG000077869.1 (SMESG000077869.1) HSP 1 Score: 347.821 bits (891), Expect = 4.685e-115 Identity = 164/166 (98.80%), Postives = 165/166 (99.40%), Query Frame = -1 Query: 233 MNLQIVPYKPLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWNTFIDEWMRPKHPFVIETILSFIFKSNDEYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHRLQRQK 730 MNLQIVP KPLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQW+TFIDEWMRPKHPFVIETILSFIFKSNDEYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHRLQRQK Sbjct: 1 MNLQIVPCKPLLLKYVDKGRLTQIYESLICGLCVMKPKKPQLWILDKLRLIRTMDYDDLQWDTFIDEWMRPKHPFVIETILSFIFKSNDEYQPTIEMLSIAYSAYNRSIISRCFNAWVIYHVSKKKRHGNESKLEEIALFHYQVKWISRIFIAWKLWTVHRLQRQK 166 The following BLAST results are available for this feature:
BLAST of SMED30008229 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of SMED30008229 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30008229 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30008229 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30008229 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of SMED30008229 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008229 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of SMED30008229 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30008229 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008229 ID=SMED30008229|Name=SMED30008229|organism=Schmidtea mediterranea sexual|type=transcript|length=778bpback to top protein sequence of SMED30008229-orf-1 >SMED30008229-orf-1 ID=SMED30008229-orf-1|Name=SMED30008229-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=194bp FKIIIIFLNFQINYCNMNLQIVPYKPLLLKYVDKGRLTQIYESLICGLCVback to top Annotated Terms
The following terms have been associated with this transcript:
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