Slc15a-7

Overview
NameSlc15a-7
Smed IDSMED30008199
Length (bp)1566
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Slc15a-7 (SMED30008199) t-SNE clustered cells

Violin plots show distribution of expression levels for Slc15a-7 (SMED30008199) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Slc15a-7 (SMED30008199) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Slc15a-7 (SMED30008199) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008199

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30008199SMESG000023324.1 dd_Smed_v4_12097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008199SMESG000023324.1 dd_Smed_v4_12097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008199SMESG000023324.1 SMESG000023323.1 Contig54531newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008199SMESG000023324.1 SMESG000023323.1 Contig54531uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Slc15a-7 vs. TrEMBL
Match: A0A0H3YK61 (Slc15a-7 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc15a-7 PE=2 SV=1)

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 340/484 (70.25%), Postives = 400/484 (82.64%), Query Frame = 3 
Query:   66 RIDKKMSSIDLNKRVVFLFFIIYLCINSSFLYAFQFIKLNINTMSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTK-TYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPH-GNRYFTPDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFT 1505
            RIDKKMSSIDLNKRVVFLFF+IYLCINSSFLYAFQFIKLNINTMSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDR+YIKFIVAFFLFT+  CYLIVYTFT N  + E      Y R  LA  G ILVVIG FLK FE+++EVIELYGII+F RQR+     ++ ++NN   V ++KVIISISKSVIVF LLIPYGICDSQ+ YAY +QSNSL   +    +F  +FW NTI++  V+ TLLIL + RF NE  FD +YS +K KIILGN  MIISTLICGALET+RLS  WT NNS++T NYKN+ ID  TY  +++SV  M+PQ+ILLSFSE+L++PTCIEYVI +SEKSTKY ++ L+ S VNIGGISD +++ M+FK +  ++FDS  K   RLD+++YIISS+SV+SLV+S++LM RF++YF+
Sbjct:    1 RIDKKMSSIDLNKRVVFLFFMIYLCINSSFLYAFQFIKLNINTMSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRRYIKFIVAFFLFTESICYLIVYTFTPNTDTVEKIWNLNYARLALAGFGAILVVIGCFLKLFEKDTEVIELYGIIQFFRQRRFAGNLDHSILNNTETVDFRKVIISISKSVIVFFLLIPYGICDSQEFYAYEFQSNSLNQSNLLFNHFKSNFWCNTISAFTVMATLLILFAVRFCNENKFDAIYSNIKTKIILGNSLMIISTLICGALETYRLSMLWTDNNSTLTPNYKNVIIDNTTYHVVDISVFWMVPQNILLSFSEVLIVPTCIEYVIDKSEKSTKYLSIVLVFSAVNIGGISDLISMSMDFKGLFRTEFDSPGKFIHRLDHVLYIISSISVVSLVVSVMLMTRFERYFS 484
[Close]
BLAST of Slc15a-7 vs. TrEMBL
Match: A0A0H3YJ38 (Slc15a-8 OS=Schmidtea mediterranea OX=79327 GN=slc15a-8 PE=2 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 8.253e-42
Identity = 153/498 (30.72%), Postives = 263/498 (52.81%), Query Frame = 3 
Query:   90 IDLNKRVVFLFF-IIYLCINSSFLYAFQFIKLNINTMSYP-YTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPIL-----LFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTKTYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPHGNRYFTP-----DFWSNTITSMIVIMTLLILLSARFLNEKAF-DKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTAN-NSSVTSNYK-NMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFTLDEFNKMEE 1532
            ++  + + F+F  ++Y  I ++FL+    I+      S+P YT ++LV ++  +++L G+LATF+    +L  +S+   +IG+        L  T      L     ++ G+ +G F+ + L  ++DQ    +IK  +  +L    F +++VY   S     ++          +++G IL+  G F K +  +S ++ ++ ++K       F  SE  +I+       ++ +ISISKS+I F LLIPYGICDSQ  +AY YQSN ++ P+ N   +      +FW  T+  + VI+   +L+  + +N+  F   L+S +K +II GNI MI+ST I G LE FRL+  W+ +  + + S Y  N TI   TY   +++V  M+PQ +L   SE+++  TCIE++  +S+KS KY  +AL ++    GG SD    I  F +    +   N      LDY+ + ++ V+  S +ISI+L+ RF   FT+ +  K  E
Sbjct:    1 MNFQRNISFVFLLVVYFFIYTAFLHVQVNIQAGSLVSSFPKYTNVLLVAIESSSVLLIGLLATFVFPVYFLLIISLSSLMIGISFFTAVAFLNNTKDIQLRLNLGGIIMFGIFYGTFRLLLLCMILDQEQSHFIKRKIIGWLGVINFSFILVYMIPS---PIQHPNLMLFPVSFSIIGIILLFFGKFRKIYAVDSNIVNVFQLMK------RFNWSEELLISEADINDSERNLISISKSIIFFFLLIPYGICDSQTRFAYQYQSNHMKIPNWNGSLSSLTRKSNFWPITLNCLTVILAYFVLILLKEMNKSQFIQMLFSSIKLQIITGNILMILSTTIGGILELFRLNIYWSYDPKARIPSIYSFNQTIGNITYKAADLTVFAMVPQIVLDGISEVIITTTCIEFMYKQSKKSIKYLGIALFLAADGSGGFSDLFFTIYTFYH----ESLENDLNNCHLDYIYFGLAGVAFFSWIISIILINRFPHRFTIVQSQKNYE 485
[Close]
BLAST of Slc15a-7 vs. Planmine SMEST
Match: SMESG000023324.1 (SMESG000023324.1)

HSP 1 Score: 799.275 bits (2063), Expect = 0.000e+0
Identity = 431/488 (88.32%), Postives = 453/488 (92.83%), Query Frame = 3 
Query:   81 MSSIDLNKRVVFLFFIIYLCINSSFLYAFQFIKLNINTMSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTKTYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPHGNRYFTPDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTYINMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFTLDEFNKMEEVNEI 1544
            M   DL+KR + LFF+IYLCINS FLY  QF+   +NT+   Y +++   V C TL+LGG+LATFLIRA YLFYLS GCF++G+ I  +K PFTD   YW   +LIG+S GLFKF+Y+VALIDQNDRKYIKF VAFFLF+DCFCYLIVYTFTSNKASDENNTKTYVRRVLAVLGTILVVIGIF+KRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYKKVIISISKSVIVFLLLIPYGICDSQQLYAYAYQSNSLRPPHGNRYFT DFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTYINMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKAT+RLDYLMYIISSVSVISLVISILLMVRFKKYFTLDEFNKMEEVNEI
Sbjct:    1 MPLADLDKRALVLFFMIYLCINSVFLYYLQFLDSELNTIQISYDSIVFDSVCCSTLLLGGILATFLIRAHYLFYLSFGCFLLGIAIFEWKAPFTDGGFYWTVELLIGISLGLFKFLYIVALIDQNDRKYIKFNVAFFLFSDCFCYLIVYTFTSNKASDENNTKTYVRRVLAVLGTILVVIGIFVKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYKKVIISISKSVIVFLLLIPYGICDSQQLYAYAYQSNSLRPPHGNRYFTSDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTYINMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATIRLDYLMYIISSVSVISLVISILLMVRFKKYFTLDEFNKMEEVNEI 488
[Close]
BLAST of Slc15a-7 vs. Planmine SMEST
Match: SMESG000023325.1 (SMESG000023325.1)

HSP 1 Score: 526.554 bits (1355), Expect = 0.000e+0
Identity = 296/441 (67.12%), Postives = 356/441 (80.73%), Query Frame = 3 
Query:  195 MSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTK-TYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPH-GNRYFTPDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFT 1505
            MSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDR+YIKFIVAFFLFT+  CYLIVYTFT N  + E      Y R  LA  G ILVVIG FLK FE+++EVIE+YGII+F RQR+     ++ ++NN   V ++KVIISISKSVIVF LLIPYGICDSQ+ YAY +QSNSL   +    +F  +FW NTI++  V+  LLIL + RF NE  FD +YS +K KIILGN  MIISTLICGALET+RLS  WT NNS++T NYKN+ ID  TY  +++SV  M+PQ+ILLSFSE+L++PTCIEYVI +SEKSTKY ++ L+ S VNIGGISD +++ M+FK +  ++FDS  K   RLD+++YIISS+SV+SLV+S++LM RF++YF+
Sbjct:    1 MSYPYTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRRYIKFIVAFFLFTESICYLIVYTFTPNTDTVEKIWNLNYARLALAGFGAILVVIGCFLKLFEKDTEVIEIYGIIQFFRQRRFAANLDHSILNNTETVDFRKVIISISKSVIVFFLLIPYGICDSQEFYAYEFQSNSLNQSNLLFNHFKSNFWCNTISAFTVMAALLILFAVRFCNENKFDAIYSNIKTKIILGNSLMIISTLICGALETYRLSMLWTDNNSTLTPNYKNVIIDNTTYHVVDISVFWMVPQNILLSFSEVLIVPTCIEYVIDKSEKSTKYLSIVLVFSAVNIGGISDLISMSMDFKGLFRTEFDSPGKFIHRLDHVLYIISSISVVSLVVSVMLMTRFERYFS 441
[Close]
BLAST of Slc15a-7 vs. Planmine SMEST
Match: SMESG000023325.1 (SMESG000023325.1)

HSP 1 Score: 519.62 bits (1337), Expect = 0.000e+0
Identity = 295/444 (66.44%), Postives = 355/444 (79.95%), Query Frame = 3 
Query:  195 MSYPYTAMILVLVDCGTLILGGMLA---TFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTK-TYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPH-GNRYFTPDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFT 1505
            MSYPYTAMILVLVDCGTLILGGMLA    FLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDR+YIKFIVAFFLFT+  CYLIVYTFT N  + E      Y R  LA  G ILVVIG FLK FE+++EVIE+YGII+F RQR+     ++ ++NN   V ++KVIISISKSVIVF LLIPYGICDSQ+ YAY +QSNSL   +    +F  +FW NTI++  V+  LLIL + RF NE  FD +YS +K KIILGN  MIISTLICGALET+RLS  WT NNS++T NYKN+ ID  TY  +++SV  M+PQ+ILLSFSE+L++PTCIEYVI +SEKSTKY ++ L+ S VNIGGISD +++ M+FK +  ++FDS  K   RLD+++YIISS+SV+SLV+S++LM RF++YF+
Sbjct:    1 MSYPYTAMILVLVDCGTLILGGMLAIVIAFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRRYIKFIVAFFLFTESICYLIVYTFTPNTDTVEKIWNLNYARLALAGFGAILVVIGCFLKLFEKDTEVIEIYGIIQFFRQRRFAANLDHSILNNTETVDFRKVIISISKSVIVFFLLIPYGICDSQEFYAYEFQSNSLNQSNLLFNHFKSNFWCNTISAFTVMAALLILFAVRFCNENKFDAIYSNIKTKIILGNSLMIISTLICGALETYRLSMLWTDNNSTLTPNYKNVIIDNTTYHVVDISVFWMVPQNILLSFSEVLIVPTCIEYVIDKSEKSTKYLSIVLVFSAVNIGGISDLISMSMDFKGLFRTEFDSPGKFIHRLDHVLYIISSISVVSLVVSVMLMTRFERYFS 444
[Close]
BLAST of Slc15a-7 vs. Planmine SMEST
Match: SMESG000023325.1 (SMESG000023325.1)

HSP 1 Score: 504.597 bits (1298), Expect = 1.921e-176
Identity = 288/437 (65.90%), Postives = 348/437 (79.63%), Query Frame = 3 
Query:  216 MILVLVDCGTLILGGMLA---TFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTK-TYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPH-GNRYFTPDFWSNTITSMIVIMTLLILLSARFLNEKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTSNYKNMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFT 1505
            MILVLVDCGTLILGGMLA    FLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDR+YIKFIVAFFLFT+  CYLIVYTFT N  + E      Y R  LA  G ILVVIG FLK FE+++EVIE+YGII+F RQR+     ++ ++NN   V ++KVIISISKSVIVF LLIPYGICDSQ+ YAY +QSNSL   +    +F  +FW NTI++  V+  LLIL + RF NE  FD +YS +K KIILGN  MIISTLICGALET+RLS  WT NNS++T NYKN+ ID  TY  +++SV  M+PQ+ILLSFSE+L++PTCIEYVI +SEKSTKY ++ L+ S VNIGGISD +++ M+FK +  ++FDS  K   RLD+++YIISS+SV+SLV+S++LM RF++YF+
Sbjct:    1 MILVLVDCGTLILGGMLAIVIAFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRRYIKFIVAFFLFTESICYLIVYTFTPNTDTVEKIWNLNYARLALAGFGAILVVIGCFLKLFEKDTEVIEIYGIIQFFRQRRFAANLDHSILNNTETVDFRKVIISISKSVIVFFLLIPYGICDSQEFYAYEFQSNSLNQSNLLFNHFKSNFWCNTISAFTVMAALLILFAVRFCNENKFDAIYSNIKTKIILGNSLMIISTLICGALETYRLSMLWTDNNSTLTPNYKNVIIDNTTYHVVDISVFWMVPQNILLSFSEVLIVPTCIEYVIDKSEKSTKYLSIVLVFSAVNIGGISDLISMSMDFKGLFRTEFDSPGKFIHRLDHVLYIISSISVVSLVVSVMLMTRFERYFS 437
[Close]
BLAST of Slc15a-7 vs. Planmine SMEST
Match: SMESG000009956.1 (SMESG000009956.1)

HSP 1 Score: 171.4 bits (433), Expect = 3.356e-47
Identity = 155/498 (31.12%), Postives = 264/498 (53.01%), Query Frame = 3 
Query:   90 IDLNKRVVFLFF-IIYLCINSSFLYAFQFIKLNINTMSYP-YTAMILVLVDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPIL-----LFKTPFTDPTLYWVTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYTFTSNKASDENNTKTYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGIIKFIRQRKSFYESEYEMINNVGAVHYXXXXXXXXXXXXXXXXXXPYGICDSQQLYAYAYQSNSLRPPHGNRYFTP-----DFWSNTITSMIVIMTLLILLSARFLNEKAF-DKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTAN-NSSVTSNYK-NMTIDKKTY--INMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKSTKYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMYXXXXXXXXXXXXXXXXMVRFKKYFTLDEFNKMEE 1532
            ++  + + F+F  ++YL I ++FLY    I+ +    S+P YT ++LV ++  +++L G+LATF+    +L  +S+   +IG+        L        TL     ++ G+ +G F+ + L  ++DQ    +IK  +  +L    F +++VY   S     ++          +++G IL+V G F K +  +S ++ ++ ++K       F  SE  +I+       ++ +ISISKS+I F LLIPYGICDSQ  +AY YQSN ++ P+ N   +      +FW  T+  + VI+   + +  + +N+  F   L+S +K +II GNI MI+ST I G LE FRL+  W  +  + + S Y  N TI   TY   +++V  M+PQ +L   SE+++  TCIE++  +S+KS KY  +AL ++    GG SD    I  F +    +   N      LDY+ + ++ V+  S +ISI+L+ RF   FT+ +  K  E
Sbjct:    1 MNFQRNISFVFLLVVYLFIYTAFLYVQVNIQADSMVSSFPKYTNVLLVAIESSSVLLIGLLATFVFPVFFLLIISLSSLMIGISFFTAVAFLNNKEDIQLTLNLCGIIMFGIFYGTFRLLLLCMILDQEQSHFIKRKIIGWLGVINFSFILVYMIPS---PIQHPNLMLFPVSFSIIGIILLVFGKFRKIYAVDSNIVNVFQLMK------RFNWSEELLIDEADINDSERNLISISKSIIFFFLLIPYGICDSQTRFAYQYQSNHMKIPNWNGSLSSLTRKSNFWPITLNCLTVILAYFVFILLKEMNKSQFIQMLFSSIKLQIITGNILMILSTTIGGILELFRLNIYWRNDPKAGIQSIYSFNQTIGNITYKAADLTVFAMVPQIVLDGISEVIITTTCIEFMYKQSKKSIKYLGIALFLAADGSGGFSDLFFTIYTFYH----ESLENDLNNCHLDYIYFGLAGVAFFSWIISIILINRFPHRFTIVQSQKNYE 485
The following BLAST results are available for this feature:
BLAST of Slc15a-7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A0H3YK610.000e+070.25Slc15a-7 (Fragment) OS=Schmidtea mediterranea OX=7... [more]
A0A0H3YJ388.253e-4230.72Slc15a-8 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
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BLAST of Slc15a-7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc15a-7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023324.10.000e+088.32SMESG000023324.1[more]
SMESG000023325.10.000e+067.12SMESG000023325.1[more]
SMESG000023325.10.000e+066.44SMESG000023325.1[more]
SMESG000023325.11.921e-17665.90SMESG000023325.1[more]
SMESG000009956.13.356e-4731.12SMESG000009956.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008199 ID=SMED30008199|Name=Slc15a-7|organism=Schmidtea mediterranea sexual|type=transcript|length=1566bp
CAAATATTTGTTTAAACTAATTAAATTAATCAAATCCTCACAGTAATTAA
TTTCAAACTTACTAAAGAATTGATAAAAAAATGTCATCAATTGATTTAAA
TAAGCGAGTTGTTTTTTTATTTTTTATCATTTATTTATGCATAAATTCAT
CATTTTTATATGCATTTCAATTTATCAAATTAAATATAAATACAATGTCA
TATCCATATACAGCTATGATTCTTGTGCTCGTTGATTGTGGTACATTGAT
CCTAGGAGGAATGCTTGCCACATTTCTAATTCGGGCGCAATATTTATTCT
ATTTATCAATTGGATGTTTTGTCATTGGAGTTCCAATTCTGTTATTCAAA
ACTCCATTTACGGATCCAACCTTATATTGGGTAACTCATGTTCTGATTGG
ATTATCATTCGGATTGTTTAAATTCATCTATCTTGTTGCGCTAATTGACC
AAAATGACAGGAAGTATATCAAATTTATTGTGGCCTTCTTTCTATTTACC
GATTGCTTCTGTTATTTGATTGTCTACACTTTCACTTCTAATAAAGCGAG
TGACGAAAATAATACGAAGACATATGTGCGTCGAGTACTTGCTGTACTTG
GGACCATTCTGGTGGTGATAGGAATTTTTCTGAAAAGATTTGAAAGGAAT
TCCGAAGTGATCGAATTATATGGAATCATAAAATTTATTAGACAGCGAAA
GTCGTTTTATGAAAGCGAATACGAAATGATAAATAATGTTGGGGCGGTTC
ACTACAAAAAAGTTATCATTTCTATTTCGAAATCTGTGATTGTATTCTTG
CTTCTCATTCCATATGGGATTTGTGATTCTCAGCAACTTTATGCTTATGC
CTACCAATCAAATAGCCTCAGACCGCCACATGGAAATCGCTATTTTACAC
CTGATTTCTGGAGTAACACGATCACCTCTATGATCGTGATCATGACATTA
TTGATATTGCTCAGTGCCAGATTTTTGAACGAAAAAGCATTCGACAAACT
TTATTCAAAAATGAAAGCTAAAATTATTTTGGGAAATATTTTTATGATAA
TTTCCACTTTGATCTGTGGAGCACTTGAGACTTTCCGCTTGAGCACCAGA
TGGACAGCTAACAATTCTTCGGTTACATCAAATTACAAAAATATGACAAT
TGATAAAAAAACGTACATAAATATGTCTGTTTTATGTATGATCCCACAAA
GCATCCTGTTGAGCTTCAGTGAGATTTTAGTAATTCCAACCTGTATTGAA
TACGTTATCCTGGAATCTGAGAAAAGCACAAAGTATTTCGCAGTGGCCCT
CATAGTCAGCGTGGTGAATATAGGCGGCATCTCTGACTTCCTAACAGTAA
TAATGGAATTCAAAAATATAACTCCTTCAGACTTTGATTCAAACAAAAAA
GCCACCCTTAGACTTGATTATTTAATGTATATAATAAGCTCGGTTTCTGT
CATTTCCCTTGTGATCAGCATCCTTTTGATGGTTCGGTTTAAAAAATATT
TCACCCTGGATGAATTTAATAAAATGGAAGAAGTAAATGAAATATAACAA
TACAACTTTTTAAGTA
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protein sequence of SMED30008199-orf-1

>SMED30008199-orf-1 ID=SMED30008199-orf-1|Name=SMED30008199-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=489bp
MSSIDLNKRVVFLFFIIYLCINSSFLYAFQFIKLNINTMSYPYTAMILVL
VDCGTLILGGMLATFLIRAQYLFYLSIGCFVIGVPILLFKTPFTDPTLYW
VTHVLIGLSFGLFKFIYLVALIDQNDRKYIKFIVAFFLFTDCFCYLIVYT
FTSNKASDENNTKTYVRRVLAVLGTILVVIGIFLKRFERNSEVIELYGII
KFIRQRKSFYESEYEMINNVGAVHYKKVIISISKSVIVFLLLIPYGICDS
QQLYAYAYQSNSLRPPHGNRYFTPDFWSNTITSMIVIMTLLILLSARFLN
EKAFDKLYSKMKAKIILGNIFMIISTLICGALETFRLSTRWTANNSSVTS
NYKNMTIDKKTYINMSVLCMIPQSILLSFSEILVIPTCIEYVILESEKST
KYFAVALIVSVVNIGGISDFLTVIMEFKNITPSDFDSNKKATLRLDYLMY
IISSVSVISLVISILLMVRFKKYFTLDEFNKMEEVNEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
GO:0005215transporter activity
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
GO:0006810transport
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000109POT_fam
IPR036259MFS_trans_sf
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 19..362
e-value: 4.6E-20
score: 73.5
NoneNo IPR availableCDDcd06175MFS_POTcoord: 107..345
e-value: 1.3604E-10
score: 60.1419
NoneNo IPR availableTMHMMTMhelixcoord: 5..27
NoneNo IPR availableTMHMMTMhelixcoord: 321..343
NoneNo IPR availableTMHMMTMhelixcoord: 42..60
NoneNo IPR availableTMHMMTMhelixcoord: 279..301
NoneNo IPR availableTMHMMTMhelixcoord: 237..259
NoneNo IPR availableTMHMMTMhelixcoord: 191..213
NoneNo IPR availableTMHMMTMhelixcoord: 152..171
NoneNo IPR availableTMHMMTMhelixcoord: 104..123
NoneNo IPR availableTMHMMTMhelixcoord: 44..66
NoneNo IPR availableTMHMMTMhelixcoord: 12..34
NoneNo IPR availableTMHMMTMhelixcoord: 71..89
NoneNo IPR availableTMHMMTMhelixcoord: 99..121
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 42..344
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 44..348